LOCUS       DACEKH010000001        73249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEKH010000001 DACEKH010000000
VERSION     DACEKH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.001"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            305..1519
                     /locus_tag="IW662_000001"
     CDS             305..1519
                     /locus_tag="IW662_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0864860.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1550..1768
                     /locus_tag="IW662_000002"
                     /pseudo
     CDS             1550..1768
                     /locus_tag="IW662_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2187..2660
                     /gene="dfrF"
                     /locus_tag="IW662_000003"
     CDS             2187..2660
                     /gene="dfrF"
                     /locus_tag="IW662_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0864861.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2787..3443
                     /locus_tag="IW662_000004"
     CDS             2787..3443
                     /locus_tag="IW662_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0864862.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            3625..4155
                     /locus_tag="IW662_000005"
     CDS             3625..4155
                     /locus_tag="IW662_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0864863.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            4701..4943
                     /locus_tag="IW662_000006"
     CDS             4701..4943
                     /locus_tag="IW662_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ0864864.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            4940..5410
                     /locus_tag="IW662_000007"
     CDS             4940..5410
                     /locus_tag="IW662_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0864865.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            5538..6752
                     /locus_tag="IW662_000008"
     CDS             5538..6752
                     /locus_tag="IW662_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0864866.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(7462..7650)
                     /locus_tag="IW662_000009"
     CDS             complement(7462..7650)
                     /locus_tag="IW662_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0864867.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            7741..7950
                     /locus_tag="IW662_000010"
     CDS             7741..7950
                     /locus_tag="IW662_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ0864868.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            8062..9675
                     /locus_tag="IW662_000011"
     CDS             8062..9675
                     /locus_tag="IW662_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ0864869.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            9710..10921
                     /locus_tag="IW662_000012"
                     /pseudo
     CDS             9710..10921
                     /locus_tag="IW662_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            10944..11870
                     /gene="glsA"
                     /locus_tag="IW662_000013"
     CDS             10944..11870
                     /gene="glsA"
                     /locus_tag="IW662_000013"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0864870.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            12078..13538
                     /locus_tag="IW662_000014"
     CDS             12078..13538
                     /locus_tag="IW662_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0864871.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            13759..15522
                     /locus_tag="IW662_000015"
     CDS             13759..15522
                     /locus_tag="IW662_000015"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0864872.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            15606..16064
                     /locus_tag="IW662_000016"
     CDS             15606..16064
                     /locus_tag="IW662_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0864873.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            16173..16856
                     /locus_tag="IW662_000017"
     CDS             16173..16856
                     /locus_tag="IW662_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0864874.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            17003..17617
                     /locus_tag="IW662_000018"
     CDS             17003..17617
                     /locus_tag="IW662_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0864875.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            17633..17839
                     /locus_tag="IW662_000019"
     CDS             17633..17839
                     /locus_tag="IW662_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0864876.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            18380..19510
                     /locus_tag="IW662_000020"
     CDS             18380..19510
                     /locus_tag="IW662_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0864877.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            19602..20357
                     /locus_tag="IW662_000021"
     CDS             19602..20357
                     /locus_tag="IW662_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0864878.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            20415..22454
                     /locus_tag="IW662_000022"
     CDS             20415..22454
                     /locus_tag="IW662_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0864879.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(22563..23714)
                     /locus_tag="IW662_000023"
     CDS             complement(22563..23714)
                     /locus_tag="IW662_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0864880.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(23868..24398)
                     /locus_tag="IW662_000024"
     CDS             complement(23868..24398)
                     /locus_tag="IW662_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0864881.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(24497..24847)
                     /locus_tag="IW662_000025"
     CDS             complement(24497..24847)
                     /locus_tag="IW662_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0864882.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(25004..26533)
                     /gene="ahpF"
                     /locus_tag="IW662_000026"
     CDS             complement(25004..26533)
                     /gene="ahpF"
                     /locus_tag="IW662_000026"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ0864883.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(26543..27106)
                     /gene="ahpC"
                     /locus_tag="IW662_000027"
     CDS             complement(26543..27106)
                     /gene="ahpC"
                     /locus_tag="IW662_000027"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ0864884.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(27250..27912)
                     /locus_tag="IW662_000028"
     CDS             complement(27250..27912)
                     /locus_tag="IW662_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0864885.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            28096..29742
                     /locus_tag="IW662_000029"
     CDS             28096..29742
                     /locus_tag="IW662_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0864886.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            29733..30815
                     /gene="uxuA"
                     /locus_tag="IW662_000030"
     CDS             29733..30815
                     /gene="uxuA"
                     /locus_tag="IW662_000030"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0864887.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(30923..32476)
                     /locus_tag="IW662_000031"
                     /pseudo
     CDS             complement(30923..32476)
                     /locus_tag="IW662_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            32624..33765
                     /locus_tag="IW662_000032"
                     /pseudo
     CDS             32624..33765
                     /locus_tag="IW662_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(33891..34970)
                     /locus_tag="IW662_000033"
     CDS             complement(33891..34970)
                     /locus_tag="IW662_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0864888.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(35073..35513)
                     /locus_tag="IW662_000034"
     CDS             complement(35073..35513)
                     /locus_tag="IW662_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0864889.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(35570..35980)
                     /locus_tag="IW662_000035"
     CDS             complement(35570..35980)
                     /locus_tag="IW662_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0864890.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(36160..36594)
                     /locus_tag="IW662_000036"
     CDS             complement(36160..36594)
                     /locus_tag="IW662_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0864891.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(36694..37542)
                     /locus_tag="IW662_000037"
     CDS             complement(36694..37542)
                     /locus_tag="IW662_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0864892.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            37931..39697
                     /locus_tag="IW662_000038"
     CDS             37931..39697
                     /locus_tag="IW662_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ0864893.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            39799..41058
                     /locus_tag="IW662_000039"
     CDS             39799..41058
                     /locus_tag="IW662_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0864894.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            41205..42506
                     /locus_tag="IW662_000040"
     CDS             41205..42506
                     /locus_tag="IW662_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0864895.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            42509..43360
                     /locus_tag="IW662_000041"
     CDS             42509..43360
                     /locus_tag="IW662_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0864896.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            43373..44188
                     /locus_tag="IW662_000042"
     CDS             43373..44188
                     /locus_tag="IW662_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ0864897.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            44339..45505
                     /locus_tag="IW662_000043"
     CDS             44339..45505
                     /locus_tag="IW662_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0864898.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            45735..46382
                     /locus_tag="IW662_000044"
     CDS             45735..46382
                     /locus_tag="IW662_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ0864899.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            46466..47410
                     /locus_tag="IW662_000045"
     CDS             46466..47410
                     /locus_tag="IW662_000045"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ0864900.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            47545..48996
                     /locus_tag="IW662_000046"
     CDS             47545..48996
                     /locus_tag="IW662_000046"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0864901.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            49017..49853
                     /locus_tag="IW662_000047"
     CDS             49017..49853
                     /locus_tag="IW662_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0864902.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            49853..50512
                     /locus_tag="IW662_000048"
     CDS             49853..50512
                     /locus_tag="IW662_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0864903.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            50638..51327
                     /locus_tag="IW662_000049"
     CDS             50638..51327
                     /locus_tag="IW662_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0864904.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            51333..52289
                     /locus_tag="IW662_000050"
     CDS             51333..52289
                     /locus_tag="IW662_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ0864905.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            52333..52845
                     /locus_tag="IW662_000051"
     CDS             52333..52845
                     /locus_tag="IW662_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0864906.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(53101..53649)
                     /locus_tag="IW662_000052"
     CDS             complement(53101..53649)
                     /locus_tag="IW662_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0864907.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(53738..54919)
                     /locus_tag="IW662_000053"
     CDS             complement(53738..54919)
                     /locus_tag="IW662_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ0864908.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(55155..56663)
                     /locus_tag="IW662_000054"
     CDS             complement(55155..56663)
                     /locus_tag="IW662_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ0864909.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            56822..57013
                     /locus_tag="IW662_000055"
     CDS             56822..57013
                     /locus_tag="IW662_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0864910.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            57216..58133
                     /locus_tag="IW662_000056"
     CDS             57216..58133
                     /locus_tag="IW662_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ0864911.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            58126..59199
                     /locus_tag="IW662_000057"
     CDS             58126..59199
                     /locus_tag="IW662_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0864912.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            59347..59865
                     /locus_tag="IW662_000058"
     CDS             59347..59865
                     /locus_tag="IW662_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0864913.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            59846..60238
                     /locus_tag="IW662_000059"
     CDS             59846..60238
                     /locus_tag="IW662_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ0864914.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            60314..61249
                     /locus_tag="IW662_000060"
     CDS             60314..61249
                     /locus_tag="IW662_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ0864915.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            61382..62161
                     /locus_tag="IW662_000061"
     CDS             61382..62161
                     /locus_tag="IW662_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ0864916.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            62154..62471
                     /locus_tag="IW662_000062"
     CDS             62154..62471
                     /locus_tag="IW662_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0864917.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            62715..64325
                     /locus_tag="IW662_000063"
     CDS             62715..64325
                     /locus_tag="IW662_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0864918.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            64482..65330
                     /locus_tag="IW662_000064"
     CDS             64482..65330
                     /locus_tag="IW662_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0864919.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            65561..66040
                     /locus_tag="IW662_000065"
     CDS             65561..66040
                     /locus_tag="IW662_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0864920.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            66073..68301
                     /locus_tag="IW662_000066"
     CDS             66073..68301
                     /locus_tag="IW662_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0864921.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            68430..70073
                     /locus_tag="IW662_000067"
     CDS             68430..70073
                     /locus_tag="IW662_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0864922.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(70146..70691)
                     /locus_tag="IW662_000068"
     CDS             complement(70146..70691)
                     /locus_tag="IW662_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ0864923.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(70714..71199)
                     /locus_tag="IW662_000069"
     CDS             complement(70714..71199)
                     /locus_tag="IW662_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0864924.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(71189..72001)
                     /locus_tag="IW662_000070"
     CDS             complement(71189..72001)
                     /locus_tag="IW662_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ0864925.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(72140..>73249)
                     /locus_tag="IW662_000071"
     CDS             complement(72140..>73249)
                     /locus_tag="IW662_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0864926.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        24509 a        12806 c        14461 g        21473 t
ORIGIN      
        1 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
       61 ttctttattc cttatgaaaa aatatcttta aaaataaaaa aagacgaaaa taaacggttt
      121 tctataaaaa gaagaccgtt tattttttgt ctttatttgt gtgaaaaacg aaaaagagca
      181 caacacatca agcttttatt aattgtctgt cttttataaa tgcaagtatt aattcaaaaa
      241 aacagaagaa aaaaacaact acaccttctt ttatactcgt aatcgtagaa aggaggcgaa
      301 aaaaatgaat acattaccta tcaatacagg agatacagca ttcatgattt tatgtacagc
      361 aatggtttgc ctgatgactc ctggtctagc ttttttttac ggcggattag cacggaagaa
      421 taatatacta acgatcatgt ctcaatcttt tctctccgtt gggataacaa cgattatgtg
      481 ggtatttggt ggattcggat tggcctttgg acgggatatc ggcggtgtta ttggaaaccc
      541 ggcagatttc tttttgatga gacatgttac aagcttcccc aacatgtttc atggtgcaac
      601 gattccattt ttaatgttct ttctttacca actgatgttt atcgtgatta ccgttccttt
      661 gatgacagga gcttttgcag ggagattgaa tttaaaaggc tatattatac ttgttatttt
      721 ttggaatctg ctgatttatt tccctgtttg ccactggata tggggtggcg gatttttgga
      781 tcagatggga tttagagatt tcgctggtgg agcagtcatc cacacgacag ctggattcgg
      841 ctctttagca tgtatcctca cacttggtca aagaaagatt cctttaggag aaagaagtca
      901 gcatagcagt cttatggcag ctgctatcgg tacaggactg ctttggtttg gctggttcgg
      961 ttttaattcc ggtggtgcac ttcgagcaga tgaacaagca gtcaatgcat ttggttcaac
     1021 attcatcgct cttgcatttg cgatgattac ttggctgatc attgcaaaag taaaaggaaa
     1081 tggctttgat tttgtcgacg tactaacagg atccgttgct ggattggcag ccattacgcc
     1141 ttgtgccggt tatgtagaag caaaaagtgc catgctgatt gggatcattg cgggtatcgt
     1201 ctgtcatgct gctgttgatt ttcgtaaaaa gaaacaatgg gatgatgcac ttgatgtgtg
     1261 gggtgttcat ggcatgggcg gattcacagg tacgattctg atagggatct ttgcatctaa
     1321 cagccattta ctaacgaatc caagcagctg gtacttctta gggattcaag tgattggtgt
     1381 catcattact gccgtgtatg cctatgtgct gacaacagtg attttaaaat cggcgatgca
     1441 ctttacaacg atcactacta caaaagaaga acaagaagaa ggattggact ttttcttctt
     1501 tggcaacaaa ctcaattgaa acaaattcaa atagaaaaga ggaagattca tgcaacaaag
     1561 caaaacgaaa attcctgcaa aaaaattaac gttctttggc tttttggcta tgaccatctc
     1621 aatggtcgtt tcattatatg aatatcccac attcgcgacc tcaggcttca gcttagtatt
     1681 tttcttgtta ttaggcggtt tgctatggtt tatccctgtg gcattgtgcg cagcagagat
     1741 ggcaacggtg aaaggatggg aaaaaggtgt ttcccaaaca aaaagatatt aagaaacagc
     1801 ccatgtttac ctgcttgcaa acaaaagaat attgctacta gactaagaga tactacataa
     1861 gtgtgttggt atctcttttt tgttgccgtg aaacagcaga ctatttcatt tctgcttgat
     1921 taccttaaaa cttattagtc tatggtttgt caagggcttg ttgcgtgaga aatgctgaat
     1981 atcacccttt acaaaacata ggctgataag taacttttca aaagcagaaa agaaatggca
     2041 atgcttataa caatatgata taatgcagat gaagacaaaa aaggaatttg tcgagctggt
     2101 tagttcgaga taattttgag tttgttaaga tgtggtgcac ttgatggaat cacaggtacg
     2161 acatcatatt atggaggatg aaatagatga taggtttgat tgttgcgagg tcaaagaata
     2221 atgttatagg caagaatggt aatataccat ggaaaataaa gggagaacaa aagcaattta
     2281 gagagttaac aacgggtaat gtggttatta tggggcgaaa gtcttatgaa gaaatcggtc
     2341 atccgttgcc taatagaatg aatattgttg tttccaccac aacagagtat caaggagata
     2401 atttagtttc agttaaatca ttagaagatg cattattatt ggctaaagga cgagatgtat
     2461 acatatctgg tggatatgga ctatttaagg aagctttgca aatagtagat aaaatgtata
     2521 tcacagaagt agatttaaat attgaagatg gagatacatt ctttccagaa tttgatatca
     2581 atgattttga agttttgata ggggaaacac ttggtgagga agtgaaatat acgagaacat
     2641 tttatgtaag gaaaaaatga attgagtaaa ttttggattt agggtgtttt cataaatata
     2701 ttgcttttta tgcatatata aattgttgta agacaaattc cagtttgtat ggggcgataa
     2761 aatcagaatt tgagagagga ttgaatatga ataaggaatg gtctgaactt aataaaacaa
     2821 tgcaggcaca aataaaaaag aaagatactt ataagagggg tattgatact ttacttactt
     2881 tacgaagtca gttaatacaa accttagtat ctttcaaaga agaattatgc agagaagatt
     2941 ttaactcaat ccccttcata aacgctgatg gttatcatag taagacgatt gcttattcta
     3001 tttggcatat tttccgcatt gaagatatag ttgtgcatac agtgataaat gaagatgaac
     3061 aagtattttt tgcaggcaat tatcaagagc gtatcaattc acctatcatt acaacaggaa
     3121 atgaactcat gaaacagcag attgcagact tttcaaaaca gcttaatctg gaagaattat
     3181 atttatatat tttcgaagta tgggaaagca ctgagaaaat gcttgaacgg ttatcttatg
     3241 atgaattgaa aagaaaaata ccgaaagaaa gaaaaggata cttagaatcg ttgaatgtag
     3301 taaacgacaa tgaaaaagca atttggctga ttgattattg gtgtaataaa gatatttgtg
     3361 gactaattca aatgccgttt tcaagacatt ggattatgca cacagaagcc tgtttgcgta
     3421 taaagaacaa gatacattca taggcaaaaa tttcagtttg tagaactaag gaaattggaa
     3481 tttaaggagg aataaagtat ggttagtgat tttataagag cagcatttcc atggatttta
     3541 atgggtttat ttgtagcaat cagttgttcc cttatgagta aaaaagagaa ataaattcct
     3601 gtttgtattt ataaaagaga aaaaatgaga aaagtagttt tatttattgc catgagcctt
     3661 gacggatata ttgcggacgg taatggtgga gtagcttggc taaatggtca tggaaatgac
     3721 aatgaaaaca tcgacactta tacagaattt accaaagata tagatactgt cttaatgggg
     3781 tggaatactt ctcatcaggt tgttactgaa ctttctccgc aagaatgggt atataataaa
     3841 tttacaactt atgtattaac acataaagag tgcaattctt cccaacaaat tcattttaca
     3901 agtgaaaatc ctgttttatt attggaacgg ctaaaacaag aagcaggaaa ggatatttgg
     3961 atttgtggcg gagcaaatct tgttcagcag ttggtaagca aaaatatgat tgataaatat
     4021 tacatttctg ttattcctac tctgttagga aatggggttc gtcttttggg caacatagac
     4081 agggaaataa agttaaggct ttgtaaaaca cagacttata acggaataac cgatttgatt
     4141 tatatgcgta gataactaga gagaaacaga aaagctgtta aaccgacgga gcgatccgac
     4201 cacgttggcg ggaaaatcct taagattttt ccgccatgct tgcaaggggg cggttcgctt
     4261 cggcggttcg gcgagcttgc gagccgttca aagcccccgt tatctaacaa ggggggcaca
     4321 aaaaacaaga aacactatac actaacaagc ggaaaacgct gtatttataa ggcaaaaccc
     4381 catttttaca gtagtgatat aaattgtacc tatatcacta tgaaaattga atagtaaata
     4441 tgagaaagac agttgatttc aaaaaataag aaattgattg tcgttttctt ttgcagaaat
     4501 ttaagtgcag gcaattatgg tagttgtcgt aattgctgtc agctcagtgt ttatcactga
     4561 tggtgtgaaa gatttacggt tggagatgtt caataagatt attggtgctt aattattttt
     4621 attgtagtgc tgatatgaga atgttataat tggggtataa aacatacaaa taaatttgct
     4681 gaaaaatgag gtatcaaaat atggaaaatg aattactgga aaacttagga agacttcaca
     4741 caactgaact tggcgttatt agaattaaaa agaacttatc tttgaatgta gaaaatgtaa
     4801 ttgaatggtg caaagaaaaa attagccttt ctaatgcgga aatcacaaga aaaggaaaaa
     4861 actggtatat aacgattgat aattgtatta taacggtaaa tgcgtacagt tataccgtta
     4921 tcactgctca caaggtaaaa tgataaattt ttctataaag gaattaaacg catttgcagt
     4981 tattgggcag gaagtagaac tgactaatta tcaaaaaaag aatattcaga ttagtacaca
     5041 gttttggaga aagtttaaca gtagtttgaa aaaagcgtat ctttcacaat caggaaattg
     5101 ggtaaaatat gcttttatgg aaagaagaaa tggaaaactt tattatttct gttctattcc
     5161 ccaaagaacc attaccccag agggctttct gtataaagaa ataccgtctt ataaatattt
     5221 ggttgtggag catattggtg ctatggataa aatatatgaa acttatggca agatttatga
     5281 agaaattatt cctagtacat cgtatattcc tataaaagat attatcctac actttgaaaa
     5341 atacgattat cgttttcatt ggaatagtga taattcagtt attgaaattt ggatacctat
     5401 tcaagattag aagcaatttt atatccatac acacaaagca gttagtctta ggattgactg
     5461 ctttttctat acagattttt aatgacaggc aattatcaat tatggtagtt gccttttttg
     5521 attggaggaa ttgaagaatg aatgataaaa actttattga agaactaaga caaaagcgtg
     5581 aggaatacgg agtaacacaa acaaggcttg ctgttgcctg tggtatcagc cgtgaatatt
     5641 acaaccgcat tgaaaaagga aaacagccat tgaatgacga attaagagaa atcatagaaa
     5701 aacagattga gcgtttcaat ccgcaagaac cactattctt attgattgat tactttcgtg
     5761 tccgctttcc tactacggac gcattggcga ttatccgtga tgtattacaa ctgaaatcta
     5821 attacatgct ttatgaagat tatggaaaat acggatatga aagcaaatat gtacttggcg
     5881 acatcaatat catgtgttcc atgcaggagc atttaggtgt tttattggaa ctgaaaggca
     5941 aaggctgtcg gcaaatggaa tgttatctgc tggcacagga acgctcatgg tatgacttca
     6001 tgctggattg tatgacggct ggtggtgtga tgaaacggct tgatttggct atcaatgata
     6061 aagcaggaat attggatatt ccaaagttaa aggaaaaata caaggctggc gaatgtatgt
     6121 cctacttccg tatgcagaaa gattacagcg gtacagaaaa atgcggtagc gatttaccaa
     6181 agaatacagg agaaacctta tatctcggtt caacaagtag cgaattatat atgtgtgctt
     6241 atcagaaaaa ttatgagcag tatgtcaaga atggcacaga aattgaagat acggagatta
     6301 agaaccgttt tgaaatacgc atgaagaacg aacgagccta ttatgcggtt gtagatttac
     6361 tgacctatcg ggacgctgaa cgcaccgctt tttctatcat caatcattat gtccgctttg
     6421 ttgacagaga ggacgacaag ccaaaaagtc aatggataac aaatgatgat tgggcatggt
     6481 ttgtagggga aaacagagag ccgatacgct taaccacaaa gccagagcct tacactttac
     6541 aaaaagcgtt gcattggcta caaagacaag tcgcaccaac cataaaaatg gtacaggcat
     6601 tagacagaga aaatcgtaca acgatactga aagatatgat tgagcaggca gaactaaaag
     6661 acaagcataa acatttatta caattagaga aatcaaccat agaagaacgt atagataccg
     6721 ctgttccaca agagaatgac ggtatttttt aatgtaattg aaaaatttca gatttagtca
     6781 gcaaagagct gttttcattc cctatgtgaa gtgaaagaag aaaaataaat tttttattaa
     6841 atctgcaaca aaatggctac ttgcgtacag atatggtgcg agaaaaggaa atctaaactt
     6901 tttttgaaaa taggtcatta aatcggggct tgctgtccct atatgaagtg aaagaagaaa
     6961 aaagttttga aaaaatgacg gaaattcgtg attagtggac agtcttctgt gaaaagagga
     7021 aaaacatcat cattttgtat tcaaacaggc attagcagtt gagtgttgat gtgaaaagat
     7081 gaaaatactt tgttctcaac ttaacaattt aaggtttccg ttccctatat ggtgcaaaga
     7141 aaatatttca ttctattgtt ggcagtaacg gtatggtttg ggtagttagg gtgtgaaaag
     7201 gaaaatattc ttttttcatc atcaacttag caaaatgaca ttttctatcc ctatatggtg
     7261 catgaaaaga agttaaaatt tttccattta aacttagcaa atggacgttt gctgtacaga
     7321 tagtagtgaa aaaggagaaa atcaaccgtt gattgccaaa atgaaccgtt ccatttgtac
     7381 taataatgga aaaagttttg gattttggtt acgtttctcc tcttttccaa cgcgatatat
     7441 aggaaagacg atttttttct ttcaatcgtt ctttgacaac tgaataaagc tattcaatac
     7501 gtttgacaga cagctagccg ttgaaacaaa tacgccacga cctttcccct gcaagagcga
     7561 gcgaaaacct atttgtgttc ttgtaaaaga tttgtcctct tttatcgcca tgacctgtac
     7621 tgtcagataa tgatactccc gtacagccac agcttgagca ttaagagcgt cgcacgcaat
     7681 gggtacggct acataagaac tatgcagagg tggaactcct gtgggctatg acaaaagccg
     7741 ttgaattgct tagaaagatt ttacagaaag ggggtatgtg ttattgataa tgctgtaaaa
     7801 acaattcaaa agaaaaatgg caagtgtggc attggcaaca gaggaaagac aaagattgta
     7861 gtaaaagagc atttttccga aaagggcaaa acaatggaag aacttctaac tgatgtcatg
     7921 ctggaaaaag caaagcagac aatcgcataa aatcggtgca gttgtaagaa atagattgaa
     7981 tgacaaaaag tacccgtgtt atactttact tgcaagcagg tattgtaaac acgggttagt
     8041 tataaaggag gataactgac aatgaaacaa cagatttaca atactgcact ttatcttagg
     8101 ttaagccgag atgatgaatt acagggcgaa agttccagca ttaccacaca gagaagtatg
     8161 ttgcgtttat atgcaaaaga acatcatttg aatgtcattg atgaatatat tgatgacggc
     8221 tggtcgggaa caaattttga cagaccgagt tttcaaagaa tgattgagga tatagaggca
     8281 ggaaaaatca actgtgttgt aacgaaagac ctttcccgtc ttggcagaaa ttatattatg
     8341 acaggacaat atacagaatt gtatttccct agtcataatg tccgctacat agcgattgat
     8401 gacggtgtag acagcgaaaa aggcgaaagt gagattgcac catttaagaa catcatcaat
     8461 gaatgggtgg caagagatac aagccgtaaa gtcaaatcag catttaagac aaagtttgcg
     8521 gagggggctc attacggtgc ttatgctccg ttaggatata agaaacaccc tgacatcaaa
     8581 ggaaaactgt tggttgatga tgaaacaaaa tggattattg aaaaaatctt ttccctagcc
     8641 tatcaaggtt acggaagtgc aaaaatcaca aagcagttaa gagcagaaaa agttccgaca
     8701 gcgtcatggc taaattttac aagatacggt acttttgcac atatctttga gggaaaaccc
     8761 gaaagtaagc gttatgagtg gacgattgct catgtcaagg cgatattgaa aagtgaggtt
     8821 tatatcggaa acagtgttca caatatgcag tctacggtat cgttcaagag caaaaagaaa
     8881 gtgcgtaagc ccgaaagtga atggtttcga gtagaaaaca ctcacgagcc gattattgac
     8941 aaggaagtgt tctatcgtgt gcaggagcag ataaaatcaa gacgcagaca gacaaaggaa
     9001 aaggcaacgc cgatttttgc agggcttgtc aagtgtgcgg attgtggttg gtctatgaga
     9061 tttggaacga ataagacaaa taaaacgcct tacagttatt atgcttgcag ttactacggg
     9121 cagtttggca aaggtaattg ttctatgcac tacattcgtt atgatgtgct gtatcaagcc
     9181 gtattggaac gcttgcagta ttgggctaag gcagtacagc aggacgaaga aaaggtattg
     9241 aacaagatac agaaagctgg caatgcagag agaatacggg aaaagaagaa aaaggcaagt
     9301 acattgaaga aagccgagaa cagacaaaat gagattgacc gtttatttgc gaaaatgtat
     9361 gaagatagag cctgtgagaa gataacagag cgaaattttg tgatgttgtc cagcaagtac
     9421 caaaaagaac agatagaact ggaacagcag ataacaagcc taagagaaga actaagtaaa
     9481 atggaacagg atatgatagg tgctgaaaag tggattgagt taatcaagga atattccgta
     9541 ccaaaggaac tgacagcacc attattaaat gccatgatag aaaaaatcct catacatgaa
     9601 gcaacaacga atgaggataa cgaaagaata caggaaattg agatatatta ccgatttatt
     9661 ggaagagttg agtaatcaac aagagtaata tttttaacta agggaaaccg gcggtgtcta
     9721 tacttgggtc agtcgcacat tgggaaaacg attcggtttt gccgcaatct ttttccaatg
     9781 gtttgagata acagttggct acttgaccat gctgtatttc ttaactggtg cattatccta
     9841 tgcaacgggt attggtgcta tacaaaacaa taaatatctg aaattggcaa tcctattgat
     9901 tattttctgg gctattttac tctcacagtt aaaaggaacc aaatacactt cattgattgc
     9961 tcggatcggt tttgtagctg gtatcttgtt gccagcatta gtcttgtttg gcttagggat
    10021 ccattatgtc gcttcaggcg ctccactaca aacgatactg tctatgaaaa cattgattcc
    10081 tgatttttcg aaattgccta cgctggttgt ttttgtttcc tttattttag catatatggg
    10141 cgtagaaaca tctgccagtc atgcaaatga aatggaaaac cctaaaaagg attatccatt
    10201 agcaatgttt gtattggtta ttctggcaat cattttagat acttttggcg gattgaccgt
    10261 cgctactaca gtacctcaac atggtttgtc tcttaataca ggggtgattc aatcgttaga
    10321 attcctatta agacacattg atccaaatct tgcatggatt gcgaaaattc ttgcgattct
    10381 tgtttgttta ggtgtcatcg gagaaattgc ttcttgggta acctctcctt caaaagcact
    10441 tcacgtagca gcagaagaag gattacttcc tgaatatttt gcaaaagaaa acacacacgg
    10501 tgttcctgct catttgatga tcgcaaatgg gatcgttgca acaatctggg cagccgtctt
    10561 tacattaagc ggcgggggga ataatctttc atttcttgca gcgatgtcat tgactgttgt
    10621 aatctacctg atgttgtact ttatgttttt cgcgggctat ttgaagctgg tttttaaaga
    10681 tgacagtctt gaaagagcct atcaggttcc aggcggaaaa gttgtaaaag taatcgttgc
    10741 ttgtttaggt cttttgactt cactttttgc tttttgtatc tcatttgttc cacctgctag
    10801 tatcgcagct ggacagcatg ggatttatga aacgattctg attgtttctt atcttatcgt
    10861 cttagttatt ccatttacga tctacgcatg ccgtcataaa ttccagcatg cagaaaaata
    10921 aaaaatgaaa gctggttatt caaatggaag taaaagaatt agaacaattg attggaaaaa
    10981 atttccacta ttatcaaaca ggacaagtgg cggattacat tccagcatta gctgaagtgg
    11041 atcccaaaca attaggtatg gcgatttatg atttggatac aaaacaactg attgagggag
    11101 gagacgccga tgtgcgcttt gccatcgaaa gcatgtcaaa agttcccgta ttgttattag
    11161 caatcaaaga caacggaata gaaaaagtct ttcaaacaat caacacggaa ccaaccggtt
    11221 ttgccttcaa ttctatcatg aatatggaaa tcaatcatcg caaacatcca atgaatcctt
    11281 ttgttaatgc aggagcgatt gcaacaacct cgttgattgc cggtaaagat gcagatgaaa
    11341 aatttgagcg aatcttgtca tttatgaaag agatctgtga tgatcctaat atcacattga
    11401 atgaagaaat ctatcattct gaatcaagaa caggtgatat caatcgctct cttgcttact
    11461 atatgaaagg taatcaaatg atcgaaggag acgtaccgga gatactcgat gcttatttca
    11521 gacaatgctc cctcaatgtc accgctacag gcatagcaaa attagctgct gtgctggcaa
    11581 ataaaggaat tgctccttgg aatggcaaac aaatcattac tgaagaaagt gctacaatcg
    11641 tcaaatcaat tatgacaact gctggtttgt atgatgaatc aggagagttt tccgtccatg
    11701 ttggtgtacc tgcaaaaagc ggtgtaggcg gcgggttgat ggcagctgtg ccaaatcgtt
    11761 acggcatagg tgtcttcagc ccggcactag atccatttgg taacagcgca gctggtatcc
    11821 aattgttgaa agatgtagtc aaagaactag acgctgacat ctttgaataa ccattcttga
    11881 aagaggctgg ggtaaaagtc caaacggctc aaagctacct acaatgaggc tgaaattgtg
    11941 agcttgtttg acttttgtcc cagtttcttt ttttattggt ttttgaaagg gcttttctaa
    12001 ataaaaagga aacaaaaata cgatttacag atatgattaa ataaaatcat aagaaaaact
    12061 ggaggcgtgt atcaggaatg ctggaaaatt acatcgagcg aaatatattt agaaaagtgt
    12121 atctgtgtga acaattattt gaattccaag aaatagacat cgaacaaaca gctatttctc
    12181 taagggtgac tacaccaacg atccttcacg atctagaaag tctagccgag tgtctagaat
    12241 attgtataaa agaacaagta agagagaagc ataagtataa actcgtattc aaacatggta
    12301 ttgctctttc tgagctcaca cagtttctgt acggtcaatc ttatttttta aagtttttaa
    12361 gttattattt taatggtata ttcacgagta cagaactggc tgatcttgaa tttatttctt
    12421 tgagtaaagt atatacaatc aaaaaaatcg tattggattt ttttaaagaa aatgattatt
    12481 tgaaaaataa agaaatcatt atcccagaat ttgactcgcg gaacatcttg ctagctttag
    12541 tacgttatgt caattgggaa ggctatgaaa ataaaaatcc gtatgtagaa gaagcttgta
    12601 acgagttgat tgattatgta gagactcatt tttttaaaag aagatattcg gaagatgaaa
    12661 agtcctttat catacgagaa atagagatcg caatcggaag aaaggcattt ccccttggac
    12721 tgaaagaaga agataagaaa agcgcagaaa cgaagccgtt atttcaatta gtctgtcaag
    12781 gattggaaga gcaaaaagga attctagact tacaagaaga agatgtctat tatatttttt
    12841 ctttgttcaa cagtagaaat tataccaatg agaacatgga gctgttaaga aaagatttcg
    12901 aagttgttta taaaaatttt gttctcaatg acctatcact gaatgaattg attttacaga
    12961 ttattagtat cgctgaaatc gacgatgcag aaaattttat tttcaagcaa tcctttttac
    13021 aatttgtccg tacgttgtgg gcagatggac aagtattttt accggaaagg atctatcttc
    13081 tatcagaaaa agaacaactt ctgtacggaa aaattgtaga aatattggaa gagtggaaaa
    13141 agaaaaatca aatccaacta agatggaatg aaaatttcat tcgaaaattc gtcaaaagtc
    13201 tttctttgat caacagtgaa caagcgggga gacaggaaat agaaattttt gttgtttcag
    13261 aaagttcagt caaacaattt ttttatcgaa cccaatttca tttgtatgcg gaagaaggca
    13321 tcgctgaaat aaatcctatt ttatttcgtt cattagaaga attgcctgat gaatgtctat
    13381 gtgcgaaaaa aagagtggtt ttgtgcgatg ctgctagcta tcaaaatgga ttagaaactg
    13441 aaaagacaaa gatctatcca atctcattaa aaggtgcaaa catccatttg tatcatattg
    13501 ctcaggaact tcacctatta aaaaaacata tttcttgatg agaccttaat taggagacct
    13561 tctacaaagc tttttaattg tctgtagaag gtttttattt gagtcctgat tcgtgcgaag
    13621 atctacttat ataacaagct ttctctgaaa tttttttaaa atgctattga cttagagtca
    13681 actctaagta gtagaataga taaagtgtaa caaatctatt ttcattaaaa gaagatagta
    13741 cggaggagag aatgtattat gagtacagta ttacatgtat tggatgcaat caatagcccc
    13801 gcagatgtca aaaaattatc tgttgaggaa atgaaacagt tggcagaaga gatgcgccaa
    13861 aaaatcttgc aaaaggacag cgatgtaggt gggcacgttg gtccgaatct aggtgtagta
    13921 gaattaacga ttgcgtttca ttatgtattt gattcaccaa aggataaaat cgtttgggat
    13981 gtttcccatc agtcttatgg tcataagctt ttaactggtc gaaaaaatgg ttttgaaaaa
    14041 ggacattacc gtgaagtgag cggatattcc aaccaacatg aaagtaaaca tgactatttt
    14101 acagtggggc acacatctac atctatcagc ttagcaacag gtttagctaa agcgagagat
    14161 ctgaagaaag aatcaggaaa tgtggtagca tttatcggtg acggctccct aagtggtggc
    14221 ttagcttttg aaggattgaa caatggtggt gttttacaat ccaatctaat cgtattagta
    14281 aacgataatg agatgtctat tgatgagaat catggcagtt tatataaaaa cttagctgag
    14341 cttcgtcaaa ccaacggtaa aagcagcaat aacctattta aagcaatggg atttgattat
    14401 cgttacgtgg ataatggaaa cgatctggaa acggtgatcc gtttatttga agaagtaaaa
    14461 gatattgatc atccgattgt acttcacgtc catacggaaa aaggtcgcgg ttatcagccg
    14521 gcaatcgata ataaaatgaa atatcattgg catgtaccat ttgatctgtc caccggggag
    14581 gcaaagcaag taacttctgg agaaacattt aatatgatca tcatggatta tttggataag
    14641 aaaatgcaaa caagtacagc tcctattgcc gcagtcaatg cagcgatacc aggcgccttt
    14701 ggactgaaaa cattccaaga aaaacatcca gatcattatt ttgacgtggg gatcgctgaa
    14761 cagcactcta tcactttcgc ttctgctttg gcttctcagg gagtacgtcc agtcgtcttc
    14821 caatcaagta ctttcttgca acgggcatat gatcaattat cacatgattt agcgatcaac
    14881 gagaatccag tgattatttt agtcggtggc ggaacgatct cagctagtga ccgaacacac
    14941 caaggaacat ttgacattcc ttacttatca agtatcccga acatcaaata tcttgctcca
    15001 gcaacaaaag aggatatgat ctctatgcta gattgggcgt tcgaacaaac agcgcagcct
    15061 gtagcaatcc gaattcctac acatggtgta gaacatggta cagctatcgc aaatgattat
    15121 agtattccag cctatcaaac agtaaataat ggaggaaaag tagcaatttt agctttaggc
    15181 ggttttcttg aattaggaaa agaaacagta aaagctttta gaaaaaaagg aatcgaagca
    15241 acgttgatca atccaggaac gatcacagaa aatgacgtag aaacattaga taaattaaaa
    15301 gaaacacacg aattagtcat cacattagaa gatggtagtt tgtcaggagg atttggcgaa
    15361 aatatcagtc gttattatgg tgattcttca atgaacgtat taaattttgg tgcagagaaa
    15421 gaattcacgg atagcgttcc tgtagggcag ctttatgaac gttatcattt aacaccaaaa
    15481 caaatcgttg aagatagtct gcgtgtcttg aatattcaat aattcattat gctcaccatc
    15541 cagataattg tttatactag tatctggatg gttttttcat tataaaaagc aaaggggtgg
    15601 atgttatgta tcagcaagta gaagaatatg tcaaaaagtt ccaagaaaaa tatcctagtt
    15661 atcaatcaga aaaattaggt ttcttttcat ttggggatac agaaaaaatg gcagacgagt
    15721 tgagcgaact agttgtaaaa aagataaaga ctggtacatc aagtggttac gatctgtata
    15781 aacaagagaa tgagccttta ccaaaagttg gtcaattaga cgtggtcctg aatggaaaaa
    15841 atgaaccagt ttgtatcatc aaaaatataa atgtgaccat cttgcctttt agtgaagtca
    15901 cgaaagagca tgcttggaaa gaaggagaag gggatcggac attagcttat tggcggaaag
    15961 tccatgctga gttttttaaa catgcttata aagaagcgtt gaacatagaa tttacagaag
    16021 agaagctggt cgtatacgaa gaatttgaag taattttctt ttagtaagaa aggtctgctg
    16081 aaagaaatag ctcattattg tctcctgaat agaaaagggt tacattttta tatgaagctg
    16141 tatttttata taacattact ggaggagata ttgtggatac actggaacaa atcaaaggcg
    16201 gagtcgtcgt ttcttgtcaa gctttggaaa acgagccgct acacagttct tttattatgg
    16261 ggagaatggc tttggctgca aaagaaggcg gagcagttgg tattcgagca aatacgacag
    16321 aagatatcaa tgaaatcaag cgaatcgttg atttaccggt aatagggatt atcaaaagag
    16381 attacgatgg gtcagaagtg tttattactg cgacagaaaa agaagtggaa gaacttttgg
    16441 agacgcaagc agaaatcatt gcactggatg caacttctcg taaacgtccg acagaaaaaa
    16501 caactgctga acttgtccaa atgattcacg aacacggtcg gttagctatg gcagatattt
    16561 cgacttttga ggaagcgctt caagcgcaag aagatggttt tgatcttttg tctacgactt
    16621 tagcaggcta tacagaatat tcacgaaaga cgcagacacc tgattttgat ttattgaatc
    16681 aaatcataga acaagtcgat atcccagtaa ttatggaagg acatactgac tctcctgaac
    16741 aagtagagaa agcatatgaa ttaggtgctt tttcagtcgt agttggatct attatcaccc
    16801 gtccgcaatt gatcactaaa agatatgtcg aagccgcaag taaagcaaat aactaaaaaa
    16861 cacgcacggc acagattctt gacaaaaaag aaaaattgat agatagttta aagcagattc
    16921 taagaagatg aaaaaagcat tacttttcat tttgtcttca agatctgctt tttaacgaat
    16981 tttatagaaa gaaggcgaaa ccatgtttga acggagtcct tcagaattga cagaacgtga
    17041 aaattacaag ttgctgattg gttcagtcat ccctagacca gtagcagtag taacgactaa
    17101 atcagcaaaa gatatagtga atatcgcacc cttcagttac ttcaatatcg tcagttcgaa
    17161 tcgacccatc ttatccttgg cgatccagag aaaacaagat gcagtaaaag acacagcaaa
    17221 gaatattctt gagacaagag aagctgttat ccatatcctt gatgaggaca acgtagaaga
    17281 agtaaataaa acagcagcca atctgcctaa tgaggaaagc gaactgtacc tgactgactt
    17341 gactttaact gattcagtaa tcatttcagt accagcattg acagaagcaa aagttcgttt
    17401 tgaaacatcc ttatatgaac atgtcgagat acggaataaa aataaagtaa cagctgattt
    17461 atttttgcta gaagtacaga aatatcattt aaatgaaaca gtctatgatg ataagacagg
    17521 aaggatcgat ccaagaaaat tgaatgctgt aagccgtttg gcaggaaatg attatgcagc
    17581 aataggagaa attttcacga ttgcacgtcc cgaataaaaa ggagcactag ttatgttaaa
    17641 aataagctat aaaccatcga cagattcaaa agaaatgaaa aaagaatatg aaacagtgaa
    17701 tgattttctg caagggcagt acttagaagt tccgccattg caagaccatt ttgtcgttac
    17761 tactgttact ttagatggta aggaaataga aatgccagat caaacgatta gcggattgtt
    17821 taattacttt aacaaataaa tatttattcc gaaaagtgaa aaaacatata aaaaaacaca
    17881 taaaactaaa aaattatcaa agaatcctgg tgcaactgaa agaatttgca acgggattct
    17941 ttcaacttat ttgttatttt tttctaaaaa atattaaaga ttgttaaatt tcactatttc
    18001 atttcaaatt ccattgccta atcattagga aaagtctatc atgaactcgt aaatagaata
    18061 tagtaataac cttgttaggt gaggctccta tacaaacata ggctgttgcc cagaaacgtc
    18121 gaaagtcgcc aatgggtaaa acagaaactg ttggattaag gctttttcca atagctaaga
    18181 atgttttctt tacgttgtat agtgctaaaa ctcggacgat gagatgatgc taggtcagaa
    18241 ttcttgttcg aaaaggtttc tgaccttttc tttatatttt tgtgactgac ttatttgatg
    18301 agacaaactc gctcctcacc tgacaagtgg attgctgtgt gaggaggaga tgcggtgaaa
    18361 aaagatcgat tggtggattt tgggtcctta tttgactttg tccatgatcg gtcttttaga
    18421 agtatacagt gccagttctt atcgtttatt gcaagccgat gaaaatacaa aaagtctatt
    18481 attgcgtcaa ctaattttca tatttttgag ttggagcgtg atcttcttag ctcgttcagt
    18541 caaactacac tatttacttc accctaaaat agcaggatac ggtttagcct tatcgatttt
    18601 ctttttagta ttagtaagaa tagggatatt cggtgtcact gtcaacggcg cacaacgttg
    18661 gatctctctg tttggcattc aattccagcc ttctgaactg gcaaatcttt ttttgatttt
    18721 ttatttaagc tggttttttc gtgacggaaa tagtagccca aaagatctaa aaaaaccatt
    18781 cctgattaca gtaggtataa cttttctgat tttatttcag ccaaagattg ctggagcatt
    18841 gatgatcctt tcgattgcgt gggtcatatt ttgggcagcg gcggttccat ttaaaaaagg
    18901 gatctatcta atcgttactt tttctgcatt gctgattgga gcagcaggcg gggtattata
    18961 tttaggaaat aaaggttggc ttccacagat gtttaatcat gcttatgaaa ggatcgcgac
    19021 tttaagagat tcttttatcg atagtcatgg tgcgggctat caaatgaccc attcgtttta
    19081 tgcactatat aatggtggca tatttgggcg gggattagga aatagtatca caaaaaaagg
    19141 gtatcttcca gagacagaaa cagattttat tttttcgatt attacagaag agcttgggct
    19201 aatcggtgca ttgtgtgtgc ttttcttatt gttctcatta tgcatgagaa tcttttgttt
    19261 aagcagtcgc tgtaaaaatc agcaagctgg attgtttctt ttaggattcg gaactttgct
    19321 attcgttcag acgataatga acgtgggaag tatcgcgggg ctgatgccaa tgactggtgt
    19381 gccattgcca tttgttagct acggaggaac aagttatctg atcctttctt taggaatcgg
    19441 aatcacgttg aatatttctt caaagataca ggccgaagag ttggcgctct atcgacctga
    19501 aaaacaataa agagaaactg agaaactctc agtttgaatt atcataaagt acaatctttt
    19561 atttttcaag cgagaggttc ttttagaaag aggaattcgc aatgaaagta tctcaaaaaa
    19621 tcatattatc tattttgtta ctgcttacat tagtagttgg ctatttttcg attgagttta
    19681 tccatggatt ttcgtctgca aaacagagtt caactgtgaa aaaggtagag ccgaaaagta
    19741 tccctaccac actaaatgtg gctttgattg gttcagatgc tcgttcgaaa gacgaaaacg
    19801 gtcgctcgga ttcacttctg gttgcacaat acaaccagaa aacacaacaa gcaaaactga
    19861 tctctatcat gagagattca tatgtcgata ttccaggtta cggaatgaat aagataaatg
    19921 cagcttactc ttatggggga gttgatttat taaatcaaac attaaaggaa aatttcaaat
    19981 ttgaagctcc ctattatgca agtatcacat ttcaagattt tatcgattgc gttaatgaac
    20041 tgtttcctgg tggagtcaag atcgatgcag aaaaatctct agatttagat ggcgtatata
    20101 taaacaaagg agagcaagtg atggatggta atactttact ccagtatgct cgatttcggg
    20161 aagacaaaga aggagacttc gggaggatca gaaggcagca gcaagtgatc aaagctatct
    20221 ctcagcagct gaaagacgta acttcaatat tcaagctacc caaagcggtt gggaaattac
    20281 ttggtagtat ccaaacgaac cttccagaaa gtgtgcttct ggattgtggt atggatttcc
    20341 tcaaagacat atgttagaat aaacaggtat aaatagtgaa ataaaggaat gacaagcaag
    20401 agaaggagga aaaaatgaaa agaagtgaca agcacggcaa aaatcgaaca ggcgcttata
    20461 ttgccggcgc agtgatctta atagcagctg cgggtggcgg ttatttttac taccagcact
    20521 atcaagaaac ccaagcagta gaagctggag aaaagacagt tgagcaattt gtccaagctt
    20581 tgaacaaggg agattataac aaagctgcag aaatgacatc aaaaaaggcg gcaaataaaa
    20641 gtgcattatc tgaaaaagag atcttagata aataccagaa tatatacggt gctgctgatg
    20701 tcaaaggact tcagatatcg aatctaaaag tagataaaaa agacgattct acttacagtt
    20761 tttcatataa agcaaagatg aatacctcat taggtgaatt gaaagatctt tcttataaag
    20821 gaacattaga cagaaatgat ggtcaaacca cgatcaactg gcaacccaac ttggtttttc
    20881 cagaaatgga aggaaatgac aaagtaagcc tgaccacaca agaagcagca agagggaata
    20941 ttatagatcg aaatggtgaa ccattagcaa caacaggcaa actaaaacaa ttaggagtcg
    21001 ttccaagtaa acttggagat ggaggcgaaa aaacagccaa tatcaaagcg attgcttcct
    21061 ccttcgactt aacagaagat gctatcaatc aggcgatttc acaaagctgg gtacaacccg
    21121 attactttgt cccattgaaa atcattgatg gagcaacgcc agaacttcca gctggagcta
    21181 ccattcaaga agtagacggc agatattatc ctttgggtga agcagctgct cagctgattg
    21241 gttacgtggg agatatcaca gcagaagata ttgataagaa tccagaatta agcagtaatg
    21301 gaaaaatcgg tcgctcaggt ttggagatgg cttttgataa ggatcttcgt gggacaacag
    21361 gtgggaaatt aagcattaca gatgcagacg gtgtagagaa aaaagttctg atcgaacatg
    21421 aagttcaaaa tggcaaagat atcaaattga caatcgatgc gaaggcacaa aaaacagctt
    21481 ttgacagtct aggaggaaaa gctggatcaa ctgttgcgac aacgccaaaa accggtgatc
    21541 ttcttgcgct tgctagctct ccaagctatg atccaaacaa aatgacaaac gggatctcac
    21601 aagaagatta caaagcttat gaagaaaatc ctgaacatcc attcatcagc cgatttgcga
    21661 caggttatgc tcctggatct acgtttaaaa tgattacagc agcaatcggt ctcgacaacg
    21721 gcactatcga tccaaatgaa gtgttgacga tcaacgggct taaatggcaa aaagacagtt
    21781 cttggggatc ttatcaagca acgcgtgtca gtagtgatgt atcacaagta gacttaaaaa
    21841 ctgctttgat ctattccgat aatatatatg cggcacaaga aacgttgaaa atgggtgaga
    21901 aaaaatttcg tacaggctta gataaattca tttttggtga agaccttgat ttgccaatta
    21961 gtatgaatcc agcacaaatt tctaatgaag atagctttaa ctcagatatc ttgctagctg
    22021 atactggata tggacagggc gaacttctaa ttactcctat ccagcaagca gcaatgtatt
    22081 ctgtttttac caacaatggc acacttgtct atcctaaatt gattgcagat aaagagacaa
    22141 aagataagaa gaatgtcatc agcgaaacag cagtacaaac gatcgtgcca gatctgagag
    22201 aagttgtgca agatgtaaat ggtacagcac attctctttc tgctttaggg attccgttgg
    22261 cagcgaaaac tggtacagcg gaaatcaaag aaaaacagga tgtaaagggg caagagaaca
    22321 gtttcttgtt tgctttcaat cttgataacc aaggttatat gatggtcagc atgttggaaa
    22381 ataaagaaga tgatgattca gcaaccaaac gagcatcgga actattgcaa tacctcaacc
    22441 aaaattatca ataaaaaatt gctttttaag tacagtaaaa ggactgtggc gttatcgtca
    22501 cagtcctttt ttcaaataaa cggttttcac gatgtctctt tcacaacttc tttttgattt
    22561 actcacactt cataaacttt ttttgtaaaa tatttgagaa caagtggaga aataagtgta
    22621 cttaataaaa tcacgataac taaaggtgaa tagtactgtg gttcgatcaa ttgactttgc
    22681 tgtccaattt gtaaaatgat caaagccatt tctccacgcg aaatcatacc agcaccgacc
    22741 atcaatgaac tatgttgaga aaaaccggca atctgagcac caaaataacc tccaagcaat
    22801 ttggtcaaaa tcgctacaag cgtcaatata gaaataaaga gtagttgttc actaaagtca
    22861 gaaaaatcaa cttctaatcc tacactgaca aaaaatactg gtataaatac tgcatatcct
    22921 aaagcctcta cattgtggta tacttcttct ttaacctttg tctgactgac agctatccca
    22981 gcaaaaaaag caccaatgac agagctaagc ccaacaagat ccgctacgta agacattcct
    23041 aaacaaataa ctagcgatac gatgataata gaagaatttg cgtacacttt ttctgcaaat
    23101 gaaagtagga aaggcgcaat ccatttgact aataagaaaa taaaaacaaa ataaattagc
    23161 tgttcaatca aaacaagcgg taaagaagca ttgctagaag cttcccccgt tagaaaagat
    23221 aggctgaaac ttagaactaa aacgactaaa atgtcatcta ccacagaagc acccaaaatt
    23281 gttgatcctt cttttgtatt cactacgttt agttctttta atacttctac tgaaatactg
    23341 acagaagttg ccgcaagaat aatcccaaag aaaatcgctt catttcccga aacttggaat
    23401 gctttgcctg tcaaccagcc aaaaacaacc gggaagatga tcccaagcaa agcaacgaac
    23461 ataccaggtc ggaaatattt tttcaaaaga gataaatcac tttccagacc agctaaaaac
    23521 attaataaga tcacaccaat ctccgaaaag tcatggacta gaatatcagg tttgacccaa
    23581 ttcaatccag cttggcctag caaaacacca acaagtaatt gtccaatcac tgctgggata
    23641 cctaatctcc gcgagatatg tgagccaatc gttgttgaaa tcaatatcag gcataaaata
    23701 ccaataaatt ccattctcat cattcctttg tataaaatat agcccgaact tagcataaca
    23761 tacattattg aaaaaatagg aaaatatact cacaaaaaaa ccaacaatcc aaaagatttt
    23821 tttggattgt tggtttttat acggtctatt ttaataacta aaaatgatta acgtatagtc
    23881 atttttctta ttctgatgaa attgataatc tcctggatgc ccatgaaaat caaaataaag
    23941 ccgaatattt ggaatagaag catcaaactt ttgaatggat tgaacaataa gacgaatccg
    24001 ccaatcaaca acagaatact actataaaca ctccaacttt tagcttttgt attcatgctg
    24061 aagaacaact ggaacaagct gttgatgata acagctaatc caagaagaat cggcaggata
    24121 gaaacgattg caggtgcaaa agcatagacg atcagagcta agatcaggaa aaaaagacca
    24181 ctgaacagac cgatactcca agaacctgtt ttccttttaa aacggtaagc ctcaaaaaga
    24241 ttcaacaacc caagcaaaac aagataggcg gtgatcagat aaccgataaa atgaaaaaca
    24301 gcagaagggt ttagcacaat cgcaatccca gaaataatat aaacagcagc tcttaataag
    24361 gcgtattttt gaatattttg tttaatcgtc tcaaacatat aagaaaacct cctttgactt
    24421 attttaacgt gaactagtca ttttaccaaa ttaaatgtta caaaatgagc gaaggagaag
    24481 aatatctctg gaaaaatcaa cttctgagga tcttttccat ttttttgcct tttgctagct
    24541 catcgatcat tttatctaaa taacgaattt ctttcatcaa gttatcctcg atgtcttcta
    24601 ctcgtacacc acaaacaaca cctgtaatca aggaacgtga aggattgggg acaggggctt
    24661 ccgcaaaaaa ttgttcgaaa gtcacttgtt tttgtagttg atattctagt tctgtttggg
    24721 aataacccgt cagccaacaa atgatttcat ctacttcctc ttttatacga tcttttctct
    24781 ccgcttttgc tacatacata ggataaacgc ttgcaaaact tgtcgtaaaa atacgatttt
    24841 gcgccataat cagtcacctt tctttttttc aaattattta ctacttatag tatataagaa
    24901 aaagagaaga aaaagcgatt attcaaaaaa acaaaaagga ttgtgacaaa attcttgtca
    24961 ccatcctttt ttccgctctc tgacttttta caatcttcat agctcaagtg cgcatcagat
    25021 aatcgaatgc tcctaaagct gcagtagctc ctgaacccat tgaaacaatg atttgtttgt
    25081 atggactatc tgtacaatca ccagctgcga aaacgccggg gatattcgtt gcgccatttt
    25141 tctcggtcac tatttcattt cttggattca tttcgatcgt tccatctagc caatccgtat
    25201 tagggagtaa gccgactaga atgaatactc ctgaaacagc tagtgtatgc atctccatcg
    25261 tacggcgatc gatataggag agagattcta cctgattcgt accgctgatt tctttcgtcg
    25321 ctgcatgttt gataacagtg acattcgcaa gagagactag tttatcctgt aagatttgat
    25381 ctgcttttaa ttctggtaag aattctagaa catatacatg ttttgctaac ccagccaaat
    25441 cgattgctgc ttcaacacca gaattaccac caccgataac agcaatctct tttcctttaa
    25501 ataaagggcc gtcacagtgt gggcaataag caattccttt atttttaaac tctttttcgc
    25561 ctggtacatt gatagaacgc cagtgcgcac ctgtcgcgat gatggcggtt tttgctttaa
    25621 gtgatatacc gttagctaag ccaatattaa taaaatgttg ctctttattt aaggaaacgg
    25681 cctgttgatt catcataatg tcgatagggt aactacgcac atgactttct acttgctgca
    25741 tcaattttgg accttctgtg taagaggtac caatcatatt ttcaatccct agtgtttcaa
    25801 cgacttgtcc gccgaaagaa tcagcgacaa gtcctgtacg gattccttta cgtgctgcat
    25861 agatagctgc actactagct gctggtccac cgccaatcac taatacatca taaggttctt
    25921 tttcaaacaa ctcttcttca atcaaagggc cagtgatttt ttcaacaatt tgttcgatcg
    25981 tcatgcgtcc gctactaaat tcttctccat ttaaaaacac agttggcact gccatgattt
    26041 ttttatcatc gatttctttt tggaacatcc caccttcaat catcgtatga gtaatatttg
    26101 gatttaatac agctaatata ttcaatgcct gtacaacgtc ggggcaatta tgacaagtca
    26161 agctaacata actgtcaaaa tgcaatgttt gtgttatttt tttgatttta agactcaaag
    26221 attcactgat tttcggtgcc cgtccactaa tttgtaacat agccagtaaa aaagaggaaa
    26281 attcatgacc gagggggata ccagcaaaaa cgattccact ttctttgcca actggattga
    26341 tagaaaaact aggtgtgcgc atcgaatctc ttttttccca agagatttta ggagacattt
    26401 ctgctatttc cttcgtaaat tcagctagtt tttgactatt ttcactatta tccaaactaa
    26461 cagaaaaaac aataggagat tcaatcagtg ctaaatattg ttcgagattt tgtttagttt
    26521 ctttgtctaa catatccacc acctaaatct ttccaacaag atcaaaactt ggtttcaatg
    26581 tttcgccact ttctttccat tttgccggac aaacttcacc tgggtgggta cgtacatatt
    26641 gagcagcgcg aatcttatca atcagtgcac tagcgtcacg accgattcca tctgcattga
    26701 tttccatagc ttgaacgatt ccgtctggat caataataaa tgttcctcgc tgagctaatc
    26761 cttgctcttc atccaaaaca tcaaaaatac gagaaatctg atgagacgga tcaccgatca
    26821 tcacgtattc gattttaccg atagcatctg atgtatcatg ccatgctttg tgagtaaagt
    26881 gggtatcggt agagcaagaa aagacttcaa cacctaaaga tttcaatgta ctgtattgtt
    26941 cttgtaagtc ttctaattca gtaggacaaa caaaagtaaa atcagctgga tagaagcaaa
    27001 gaatactcca gttccccata agatcttttt cactgacttc taaaaattct ccttgatgat
    27061 aagcatgtgc attaaattct tcgatttttt tcccaatcaa tgacatagta aaacctccaa
    27121 taataattat ttatttataa ttattgtaat ttagttggag ttgcaaagtc aatggatatt
    27181 ttgttattta ataaagatta ctcaaaaacg aaggttaatt ataaaaatag aagaggtcgc
    27241 aagaaaatgc tactgctgga aatactcggg ataacgttga gtaacagttt cctgttcttt
    27301 cagcatcatg tgtaagtgca atcccgtttg aaatcgagct tcttctggtt ttttatcaac
    27361 taaagccttg agtaaatcat aatgttgttc catgatcgtt tcccatggaa gcccctgaat
    27421 ggtcactcgt aaccagcgga aacgttctaa atgggtatta tggtcatcta accagttcca
    27481 aacttctttt cttcctgcaa tttcgaaaca agcttcatga aataaattat ctgcacggaa
    27541 aaaagcgcgt tcgtcattta ttttgactgc tttttcttgt tcttctaaaa tcgtttctag
    27601 cactattcgt gtttgtgtgt ccaatttgga acaacattca atcatgattt gctcttccaa
    27661 ctgttctcgg acaaagcgag cattttctgc agaaacaaga ttaattttgg aaacatacgt
    27721 accactttga ggtatcgtat acacgagttc ttgctgacgt agttgaatca atgcttcgcg
    27781 aatgggagtc cggccgatag caagatgttc ctccagtgcc ttttcagata ttttccttcc
    27841 aggttctaag tcagcataga taatttgttt acgaatcgct tgataagctt gtgattgcaa
    27901 gctgcttttc atctttattc ctccaaaatc atttctatga acgtagtata acagaagagg
    27961 tctgaaaaac aatctggtat gtcacattaa ctagtaaatt tgtataaata gtaaaatttt
    28021 ggttgacata gcgattttta ttgcataaaa ttcacttgaa tacaaaaact aatatgttag
    28081 tttaggaggg gaaaaatgat tgatttaaac aactggagga actttatccc tgaattatct
    28141 caaaaagaag tagaaatacc tcattttgac cgtgcatcat taagagaaaa tggacaaaaa
    28201 aatcctgtct ggatccattt cggtggcgga aatctttatc gaggatttca tgcagaaatc
    28261 gctcaacgtt tagcggatct aggcgaattg tccactggtg tcattgtttg tgaaacattc
    28321 gatgaagaaa taattgaaag ggcttaccaa gattttgatg atttgattct tgaagtggtc
    28381 atgccaagca gtggagagtt cgtcaaacgt atattagctg cgacagctga cagtctgtat
    28441 tgtcatccag attgttctga acactttgat aagatgattc gacactttga aaatcctagt
    28501 ttacaattag ttacttttgc tattactgaa aaaggttatc gattgaaaga tacaaataat
    28561 aaatatatag gaccgattgc agtcgatata aaaaatggtc caaagaagcc atcacataca
    28621 atgagtattc ttgctgtgct actttataaa cgttttttat ctggagcttg gccgattgca
    28681 cttgtctcta cagataattt ttcacaaaac ggtagtcaat tacgtacttc tttgctaacg
    28741 atcgcaaaag aatggcaaaa ggaaggttat gtacaaaata catttatcca atacttatcg
    28801 aatgaagaaa ttgttgcgtt tccatggacg atgattgatc gtatcacccc gaatccttcc
    28861 ttgtcagtta gcaaaatgtt ggcaaaagag ggcttttcaa atatgtcgat tttccaaacc
    28921 gcaaaaggaa ataatattgc tccatttgtc aatacagagg acatccatta tttaatcatg
    28981 gaagatcatt ttcccaatgg acgtccgaaa ttcgaaaaag ctggggtatt cttgactgat
    29041 cgtgcaacgg tagataaagc agatacgatg aaggtgacga cttgccttaa tcctttgcat
    29101 acgtctttag ctattatggg ttgtttactg gggtatagga gcatagctga ggagatgaaa
    29161 gatccagatt tatcgctgct agtttctgaa atcgggtaca atgaaggcct cccagttgtg
    29221 gagtctccag gtatcattga tccaaagatc tttctggaag aagtaattac tgtgcgcttt
    29281 tctaatccaa atattcctaa tatgcctcag cgaatcgcca cggatacctc tcaaaaggtg
    29341 gcgattcgtt atggtgagac gattaaaaaa tatatggaaa tagaagttaa agacccgcag
    29401 acacttgtat tcataccatt gactctcgct gcttggcttc gataccttat tggcattgat
    29461 gatcaaggaa aaccattcat cccgagtcca gacccactat tgacagacct tcagcataag
    29521 ctttccgata ttcaactggg tacgcaagat acatcacata ttcatgagaa aatggctcct
    29581 attttgtcaa ataaagaaat ttttggtgta gatctttacc aagcagagct tggcagaaaa
    29641 atagaagaaa tgttcatcaa gatggtagca ggaccaaatg ccgtacgaaa aacattacag
    29701 gattacttac aagaacatgg aggaaattta tcatggaaat gacttttaga tggtacggat
    29761 cacaggaaga aaagattacc ttagatgaaa tcagacagat tcctgcggtc aaagggattg
    29821 taggcacatt gatggatata ccagtaggag aggtatggcc tagggagagg atcaaagaac
    29881 taaaagaaga aattgagtct cgtgggttaa cactaaaagt aattgaaagc gtcaatgtgc
    29941 atgatgccat taaaattgga ttaccttccc gtgagcagta tatcgaaaac tataagcaga
    30001 caatccgaaa cttggcagaa tatggaatag aagtcatctg ctataatttt atgcctgtat
    30061 ttgactgggt gaaatctgac cttgactacc gcttagaaga cggctcatcc acgttagctt
    30121 ttatatctga tgatattcca gatgatccaa gagaaatcgt cgcgcgcatc gaacattcca
    30181 gcaatgaatt tggattgccg ggatgggaac ctgagcgctt agctcctatc aaatccttgc
    30241 ttgaagccta tgcatcagta gacgaagaaa aacttcgaga aaattttgcc tattttctcc
    30301 aatcaatcat cccaacatgt gaagaggtag gggtcaaaat ggctgtacat ccagatgatc
    30361 cgccatactc gatttttgga ttagctcgaa tcgtaaagaa tcgagaagat ttagattggt
    30421 tgtgtcgcat agtagacagc ccttataacg gaatcactct ttgtacaggg agtattgcag
    30481 aagatccaga aaatgatgta tatgggattt tagcagaatt ttgccaaaga gatcgaatcc
    30541 catttgcaca tgttcgaaat attaaatttt tagctaatcg agatttctat gaggcaccac
    30601 ataaatccga atacggttca ttagacatgt accgcatact aaaaacaatg tatgacaacg
    30661 gatttaacgg atacatccgt cctgatcatg gaagaatgat ctggaatgag acaggaagac
    30721 caggatacgg cttatatgat cgagcattag gtgctgttta tctaaatgga ttatgggaag
    30781 caatcgaaaa aggaaacaaa agtaaagata attagtgaaa aaaactgtaa ttttattatg
    30841 taaataagaa ggatgttcaa attcttgata cataaatccg gaggttgtta ataagcattc
    30901 ggatttatgt atcttatttg gtctaggaag taacttgatt ttcttttttc gattgatcca
    30961 tgagtgcttc ttcttttaat tcaaagatat attctgagat cctcataatc acttttttcc
    31021 agtcttctgg tgtgatgatt ccttttaaaa tatagatttt tttgatatga gaaggcaatt
    31081 gtttcaaagg gcagtcgctg ataacgatgt tgcagttctc cataaaatca ttttgtattt
    31141 tgatcgattc atttgaaaaa atttgttcta tcttaacgat cagatcttta ctgatataaa
    31201 aatcacgcgt atagttcaca ccaatcacaa ttcgcggctg ttttttgtag cgatcataca
    31261 aatgtactag gtcttcaacc atccattgca cattttcctt ttctaaaata actggtaaca
    31321 agtgttgatt ttgttctctg aaatccatat aaaattgttc aatggcggtt tggacttctt
    31381 tcaatacgct atgttcttga taaagcatga aagtaggaag gggcgtattt ggttgattga
    31441 agttaagaaa tccattataa agtttatttc gtgcgaatgc caatgagtaa agaatctttt
    31501 ctttttcagg tataaacaaa tcaaatacag tacaaaggga ctccaccatt tctcttgaat
    31561 aaagaacatg aggaacgttt gcttttttta aaagtttgta ttgctccaag cttgtttctt
    31621 ccgattcgat tttcgtgata gaaagtcgag ctaaaatatt cactaagatg cgttgttcgc
    31681 gacaaattgg cagattaggt cgcaaaatat ctacagagag atcaataaaa aagtcaatat
    31741 attcatactc tgtgaattct ttttgtacgt ctttcacaga agttgcagga aaattctctt
    31801 tacatatttt taatagaaaa tataatttat ctatcatacc gctatttagt cggtcaagta
    31861 cttctttttt aaaaaaagta ttcatcataa aagccactac ttgcggatct tcatcataaa
    31921 attctgtagc agtacttgaa aagatgttcc aataaatatc taatgctgca tattgaattt
    31981 gtacctcttt tccagaaaaa gactttctgc cattattaga aaaggagata gacaaaccgt
    32041 actatgctaa aaatgcatta atctttttca ttttcgtata caaataagag gcactatagt
    32101 ttgtttgctg actgaaactg ataatcgaat ttgtctgaca gctgaacaaa gaagtcaaga
    32161 tctgataact gactgattcg ttgcagtatc ttccaaatag acgataatag cagtaagccg
    32221 gatcaattcc ttcaaaattc aactttaaaa aaggtttttg cttatcaata tgcagttctg
    32281 ttagattgag acgctcaaag tcttgttcta attctttgat gtatagtaaa aaatttgatt
    32341 cagacatatg gaaatgatct gctaaatatt gtgtagtaat aaagaaacga tgttcagata
    32401 ggaaaagaaa aatatctaat tttgtattgc tttttttctt caagataaac gttttaatcg
    32461 cttcttgagt ttgcatgaaa gaggctcctt tttacttaaa ttgttaaaac ttgaaaatat
    32521 tactgttttt ttcataaatt tagacaaaaa aacagtaaac atataggtag aatctctact
    32581 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
    32641 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
    32701 agaggagaca gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
    32761 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
    32821 agcgaccatc aaagcaaaaa ttgactattc gggagactta gcacctatac tacaatcagc
    32881 tgttacattt gagaatgttg atcccacttt gtcgattggc tatgacgaga acactttgac
    32941 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
    33001 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atttaacgcc
    33061 gtatagtcag cggcagacaa ttgagcaaga ttcagccatc gactcaggag gagatcttgt
    33121 tgaagaacca acagataaac cagaaaatga aaataagcca gaagtaccgc ctacagaaaa
    33181 tccagacgga gaacagaaac cggaaataga acctggagaa gaacccgata cagaaactca
    33241 accagagcca gacaataaat caaaacctga aataacccct ggagaaaaac cagatgtaac
    33301 tccagaacca gatacggatt ctggaaacca aacagtaccg gaaacaaatc cagacacaga
    33361 taatgaaact gaaaatccag aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac
    33421 tccagaacca gatacggacg caagagacca aggaatacca gaaaaaataa acaaaaaaac
    33481 aattcaagag gatggaaaaa aagagagtaa aaaatcaaat ctagcaattc taaaaataaa
    33541 tgaagagcag ctgaataaaa aatcacgaat atttgatagc gcccaatcag ctgaaacctt
    33601 aaaaagtagc aaggatacaa cctttgctgt cctgaaacga aaaataaaca gctaccaaaa
    33661 tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca
    33721 acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc
    33781 tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa tgaccccaaa ataccagatt
    33841 gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt ttaagggata
    33901 tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc taaaaagaag
    33961 gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc gctaacaagt
    34021 aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa cttcggattc
    34081 agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata aacaacatct
    34141 cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat gataacacct
    34201 gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag ctgatgaaag
    34261 ccaaatacag ccaaggcgat tgtagtgagc aacgtattta aaagagcgac aacaaattga
    34321 gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta aatatcttga
    34381 aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt ttttttcttt
    34441 tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc catttttcct
    34501 atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg
    34561 tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt tggctgatgc
    34621 tcgtaaaaat taataacgga ctggatcatg tcaaatgttt gatgaataag catcgggata
    34681 tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat gatagctaaa
    34741 acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat catcaaataa
    34801 gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa aagaacaaaa
    34861 acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa acgctgataa
    34921 acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat tatatgatgg
    34981 aatatgaaaa taagaaagag caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg
    35041 gttttcctca taaaatcgtt catctagcag tttcagccct ttttctggtc ttgatgaaaa
    35101 ttatctagca tttttcgggt cagatgttgc agtgtatcca attcttcctg ttcaagtgga
    35161 agataagact tgatcttttg tggaatacct tctgcttgtt tttgcaacgc agcaccttga
    35221 gcagtcagcg taacatttac gattctttca tcacccgttt cacgctgacg attaatataa
    35281 tttttttctg ctaacttttt tagtaaggga gtcagcgtac cagaatccag acacaaatca
    35341 tttcctagct ctttcactga agtctttttc ttttcccata aaactaacat cacaagatac
    35401 tgggtatagg taagattcag cggcttgagg aggggagtgt agtgtctaat gatttcttta
    35461 gaggcggcat ataaaggaaa acatacttga ttaactaaat atagatcttc catttttcca
    35521 cctccgctgt tttactatag tgtatcatag cggatttaca caaaaaagtc tattcactaa
    35581 ctgctttcag agtaacttct atatttccag ctgtagcttt tgaatacgga cacactttat
    35641 gcgctgcctc taataattct tgcgtttctt ccagagaaag accttcgata gatccttcaa
    35701 tctctacacc taagacaaag ctcgtttcac tttctgaata aagggaaact tgtgcactga
    35761 cagtagaagc cccagagata tttttttgct gtttgatcaa ttctaatgca ctgttaaaac
    35821 atgcactgaa accagctgca aacaattgtt cagggttggt tgcattttcg acgcgtttac
    35881 caggagctgc aatattcaat tcgaatgtac gatcgggatt atgcacttct ccagagcgtc
    35941 cgcctgtgtt gatcattttc gtagaataca tttttttcat aaaattacca cctttaatta
    36001 tattttatac aattgaattt tacacaatat aattgtggaa agcaaagaaa tggattggca
    36061 taagataaat aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc aaacatatac
    36121 aaaaaatatc agagacaaaa aataatctca tcataaacat tacatatatt tctcaaaaag
    36181 ttgttttctt tcttcaaaaa ttacttcacg atggacctga tgaagcatcc ataaataacc
    36241 aaaaggatct ctgaacattg agttagtcac accaaagtcc atcatttctg tcatttcttg
    36301 aatctctaca cagccatttc gtaatgcttt attatgtgtg tcctcgatat cagagacgat
    36361 gatattgaac cagatcgtat ttggatgttc agcagtagga gcagtcaaac caagagacgt
    36421 gttttcatcc agcatatgga atctcgtacc atacaaggta aaaatgactt cattattgcc
    36481 tctagggtaa tctgttgcct caatcagttg tatttcatca aagattgttt gatacagttc
    36541 tagtgctttc aaactatcat ttaatacaaa atcgatttcc gttcctttat gcataaagat
    36601 ctcctctcct tttcagtcga acagttacta gattctttta ttataccagc taaagaaaac
    36661 gcattcaaaa aaatagcgga tttacttacc aactcattcg attggatgat aagtaaatcc
    36721 gcgtcccatt acttctgcta cgctgctgac aatgacaaag gcacttggat cgatttcttc
    36781 tatcaatggt ttcattcggg aaaattcatg atcagtcaca acgatcaata aaacctcttt
    36841 tgactttcga ttatacccgc cttttgcctc tagcagggta aggccacgtc caatctcagc
    36901 acttaagcga aggcggatgt cttccaaata atgttcactc atgaccataa cagatttctt
    36961 tcgattgata cctgtttcga tataagtcat gacaattgaa gtgattgcaa tagataaaat
    37021 agcataaaga aactcttcta ttccaaaaat aaacagatta agagaaacaa cgaatgcgtc
    37081 tgtaaaaaat aagccaatcg aagtattcaa gtggaaatat tttttaaaaa tgaggggagg
    37141 gatcgtcgta ccgccgctag aagagttgtt tttatataaa atcgcaactc ctaaagcaaa
    37201 aatcgcactt ccaaaaatga cagacaacaa ccgatctgaa gtaatcattg tttctggaac
    37261 tattgcaatg attaaaggaa aaaccaagct tccgaaaagt actttaaaaa agggtttttt
    37321 acctaaaaat agaagagcta gtaccaacat aacgatattc aacactaaaa taactatagc
    37381 tcggtcaaag cctaaagcag cttccagtaa tattcctaga ccgctgacac ctccagcagc
    37441 aatatgatga ggccctaaaa acatattgat agcgactcct aatatgaaga gggcaaaaat
    37501 aatcttaatg tatgtccata taaattgtct cgtttctttc atttattcac ttccttaatt
    37561 cattaataat actcttatct tattataact tacttttcac cttaagttaa ctttaattaa
    37621 taaaaaaatt aggctagtct aaattattat gtttcattct tctagaatat acttctacgc
    37681 caaaaactgt taaaaaactt ttactttaga aaattggaga aattgatcgc ttaagataaa
    37741 ttcaggacac taaatagtac aggaagtaga ctaaaacttt taaaagtttt aagtctatct
    37801 atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa ctgttaccga taacattttt
    37861 gaaagcgttg tcttttgctt ctctatcact tatagtgatg acgtcatatt aaaacacaag
    37921 gaggaaatca atggatacag ctgcaattta tcatcgaccg gaaagtgaat atgcatttct
    37981 atatacgaat acaagatttc gtattcggct tcgtactagg aaaaatgata tcaaaaaagt
    38041 atatgtgcta tgtggagatc catataccat aacaactgaa aaatggtatc aaaaacaacg
    38101 accaatgaaa aaatgtctaa gtaccaatgt tcatgattat tgggaaattg aagtgacgag
    38161 cgaaacaagt cgcttacagt atgcgttcca tgttgttggg gaagataaca cggactgttt
    38221 ttacggtgat caaggaattt ttccttatcg agaaaatgtg attactgaac caaattttta
    38281 ttttcgaata ccttattttc atcaaatcga tcgattcact attccagaat gggtaaaaga
    38341 aacagtatgg tatcaaattt ttcccgaaag atttgcaaac ggggacaaag caaatgaccc
    38401 cgaacacact ttaccttggg gaagtaaaga tccagaccga gaagactttt tcgggggaga
    38461 tttgcaaggt gtgatggatc atttggatta cttgacggac cttggcatca atggcatcta
    38521 tttttgtccc atatttaaag ccacatccaa tcataaatac gatacgattg attattatga
    38581 ggtggatccc gcttttggcg ataaaaaact tttaaaaaac ttgattgatg aagcacataa
    38641 aagagggata cgtatcatgt tcgatgctgt attcaatcat atgggtgtcc attcaccaca
    38701 gtggcaagac gttctcaaaa acggagaaaa atcgatctat aaagactggt tccacatccg
    38761 ttttttccca gtagattctt atcagatgac tgacttacca gaaactgctg aaaatatccc
    38821 atatgatact tttgctttta ctccattcat gccgaaacta aatacagcaa atccagaagt
    38881 gcaaaaatat ttgctggata tcgcaactta ttggataaaa gaattcgata ttgatggatg
    38941 gcgtcttgat gttgctaatg aagtcgatca tcatttttgg aaaaaattca gagaagcagt
    39001 tacagaaatc aaaccagaca tctatatcct tggagagatt tggcattcct ctcaagcttg
    39061 gctgcaaggg gatgaattcc atgcagtgat gaattatgca tttacagatt cgattaaaga
    39121 ttattttgca aaaaagaaaa tcacagcttc acaaatggtc agtggaatga atcatcagca
    39181 aatgctttac agagatcagg tgaacgaagg aacattcaac ttattagatt ctcatgatac
    39241 tgccaggatc ttgacccttt gccaaggtaa caaagaattg atgaagtctg tgatggcctt
    39301 tatgtttctt caaaaagggt ctccttgtat ctattacggg acagaaatag ggatgactgg
    39361 tgaagatgac ccggattgcc gcaaatgtat gatatgggag aaggaggaac aagatctaga
    39421 gctattcggt tttataaaag aacttgtttc tctaaggaaa cagttgtcta agattatttc
    39481 tgaaggttct actcagtggc taatagtaaa tgatcgtgaa gataagctct atttcacacg
    39541 gaaactagag ggacagatca tctatgttta ctttaatcag tcaaaagaac cttgggtagt
    39601 agaacaggaa aatgaggtga ttcttagtca aaattgtcag ttgttagaag acgggaaagc
    39661 agagatacga caatacggat ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag
    39721 aaagcgtttg caaatgaaga tttgtgccca ttataataag ggtgtaacag cacgttgaat
    39781 acaaaggagg aaatgaagat gaaaaaaaga agtgtgaaac agttgggttt agggttaatc
    39841 tctttaggaa tcttaggcgg attagcggct tgtggcaatg gtaactcttc agcagaaggg
    39901 gaagccgatg aaggaaaaac gttaacagtt tccgttgatt ctggatataa agattatata
    39961 aacgaaataa aaaacacttt cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag
    40021 gacatgttcg atcagttgga atcattagcg ttagatgggc ctgctggaaa gggacctgac
    40081 gttatgatgg cagcatatga ccggatcggt gcgttaggac agcaaggaca tctggcggaa
    40141 gtaaaactgg gaaatgaagc agcttacgat gaaacagaca aagcccaagt gacttatgat
    40201 ggaaaaatct acggagaacc tgcagtaatc gagacccttg ttttgtacta taacaaagat
    40261 ttagtcgata cagcaccatc aacgtttaaa gatttggaga atctatccaa agacagtcgt
    40321 tttgcttttg agtcagaagc aggaaaaaat acaggatttt tagctaaatg gactgacttt
    40381 tattattcct atggtttaat tgcaggttac ggtggctatg tatttggtga tgatggcaca
    40441 aatccttcag atatcggttt gaataatgca ggtgctgtag aaggaatctc ttatgcaaca
    40501 gattggttca aaaatgtttg gccaaaggga atgcaagaca taaaaagtgc aggagacttc
    40561 gccagccagc aattcatgtc caataaaact gcagcaatca ttgacggtcc atggcaagcg
    40621 caaacataca aagaaaacaa tatcaattat ggtgtagcca aaattcctac tctaaataat
    40681 ggacagcctt atcaaccgtt tggcggagga aaaggatggg tagtcagcaa ttatgctaaa
    40741 aacaaagacc tctctcaaaa atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa
    40801 ttctttgaaa tggtaaatga aatccctgca aaccaacaag ctagggaaac agcaaaggct
    40861 aaaaacgatg aattaacaac agctgtgatt gaacaatatg aaacagctca agcgatgcca
    40921 aacatcccag aaatgggcga agtttggaca ggtgcagaaa acttgatgtt cgatgcagca
    40981 tcaggttcaa aaacaccaaa agaatccgca gatgaagcag taaaaacaat cagtgaagcc
    41041 atagaacaaa aatactgatt aaaatgataa ccgtccattt ttataaaaaa tggaacaggt
    41101 gcttcagata aaagtggcaa gagcgagatg gaaaattatc ttgctcttgt tgccattatc
    41161 ttaaaagcag tttgcggttt agcaagttta gagggggcat tcagatgcag caagataaaa
    41221 caagcaatat caacgaaaaa aaaatcacgc tttgttcact tattccaggg atggggcagt
    41281 ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc catattcgtc ctattcattg
    41341 gtcagatgct tctgggcgga tttcatgcat tggtaggatt ggttactttg ggaagtatac
    41401 caatggaaga tcattcatta tttattttga ttgaaggcac tttgcaattc atcattacaa
    41461 tcattttctt gattttttac ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc
    41521 gtcaagaggg caaaaaaatc aatcggacga tgaaggaagt catacataat ataggtgatg
    41581 aaggttttcc ttatctattg acacttcccg cttatcttct catgattttt acgatcattt
    41641 tcccagtcct agttacattg ttcactgcat ttactaatta tgacttcaat catattccac
    41701 cagccagttt aattgattgg gtaggggcta aaaacttttt tagtatcttc tttttaagct
    41761 catatcgtaa tacttttttt gcagtctttt cgtggaccgt gatttggact ttatgtgcaa
    41821 caactttgca aatcaccctt ggtattctca cagcggttgt tgctcaccag aaatttatta
    41881 aattcaaacg tattttcggt atcattttct tattaccatg ggcggtccca gcttttatta
    41941 ctatcatgag tttttcgaat atattcaacg atagcgtagg tgccatcaat actcaagtga
    42001 ttcctttcat aaaccagctt ccttttgtag atatcagtgc aatctcttgg aaaacggacc
    42061 cgaactggac gaaagtagca attatcttag tgcagggctg gttaggattt ccctatatct
    42121 atgtgatggt tacaggaatc ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa
    42181 tcgacggagc aaatgcatgg cagcgctttt caaacatcac gcttcctgct gtcttcctag
    42241 tggcagcgcc aacatttatt acacagtata cggggaattt caataatttc tccatgatct
    42301 atctgtttaa caatggcggt cccggaagcg taggcggagg agctggttct actgatattc
    42361 ttatttcctg gatctataaa cttacgactg gaacttctcc gcagtattct atggcttcag
    42421 caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga
    42481 caaatgcgtt caatatgggg gattaacaat gaaaaaattt cattcgagtg ctcgattcca
    42541 aaaacgactt tctcactttt ttacttactt ttttctttta atactgtcag tcattatcat
    42601 ctatccgctt ttgatcacag caagtactgc cttcaagtca ggaaatattg cagcttttag
    42661 tttagatctt tcaagtgact ggactttagg aaactttact cggctattca gtgaaacact
    42721 ttacgggact tggtataaaa atacattgat catttctgtc ctaacaatga tcgtacaagt
    42781 gactgtagta actttggcgg gctatactta tagtcgctat cgttttatcg gccgcaaatc
    42841 aagtttgatg ttctttttag tgattcagat ggtaccgact atggcagcat tgactgcctt
    42901 ttatgtcatg gctttcttat tagaagcgct cgataagtac tggttcttga caatgatcta
    42961 tataggcggt ggaattccaa tgaatacttg gctgatgaaa ggatactttg acactgtacc
    43021 aatagattta gatgaatctg ctaaacttga tggggcaggg catttcagga tttttttcca
    43081 gattgtatta ccgcttgtca aaccgatgct cgccgttcaa gcattatggg cttttatggg
    43141 accttttggg gatttcatgt tagctaagtt cctgcttcga acacctgaaa atcagactgt
    43201 tgctgtcggc ttacaaacat ttatttctga tgtgacaaac caaagagtct ctctttttgc
    43261 tgctggggca attttgattg cactaccgat ttgtctatta ttcttctttt tacaaaagaa
    43321 ctttgttacc ggcctgacat ctggcggaac aaaaggatag gagcagagta tcatgcaaac
    43381 atttccgtta aactattttt ccgcattacg tacgccaact caagtgtttg cgggacgcaa
    43441 actattatca tggcctaaat ttttcttgat atttgtattt ttagtttcct tgatggtcat
    43501 gcctgtgact cttttttatg ccaatcagat ccaagcgatt ccattagaac aatttctatc
    43561 cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt tctgaacttg aattatctga
    43621 aactgggtta caagcatcgc aacaaacgat agctgtgacc cctgaaattt tggtaggtgt
    43681 gtctttatct gagaaacaac aatccgatca tggaactttt attgattttg agaaggaaca
    43741 atgggtgatc caacaaaagg ataaatctgg tatacgccgc tatacgatga attatagccc
    43801 atctttccaa ccagattctg tacgtacgcc cgaagatttc caacgatttt tagaaagaga
    43861 attttatgcc agtaatcgtc caacaatcat tttatcttat agtttaagtc ttggtcttgt
    43921 cctatttgtc atgacgagtc tcattttatt cggggcttcc tttttcttgt ggatgacaag
    43981 aaaaagccaa ctttcatcca ttcatacatt caaagagagt gcaaatttga tgctgaatgt
    44041 tatgggaata ggttccatgg cagcagcagt tgtagggttt atccactttg actttatctt
    44101 gatgctaagt gtccaaacaa cgataactgt cttgctgctt ttatgggttt ttgtcaaaac
    44161 aagattcaaa gataagcgtg tcgaatgaca agtttaagcg atttatgcga caatgaatgt
    44221 gaaaaaaatg aaaaataaag tagtttttga aaaatttctg aaaaaattaa acttcttagt
    44281 tgattctttt cttttttagt attaacgtaa tagggtagtt tttttaagga ggaaaatcat
    44341 gttagagaaa actttataca atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt
    44401 aaattattgg gacggtacaa cgaaacaata cggagatacg gacactccta cccaaatcaa
    44461 aatcaatatc catgaagaaa tcccaatgaa agagattaca agtaacgcat ctttggcact
    44521 aggagaagcc tatatggatc atcgaatcga gatcgaggga agtatccaag cattgatcaa
    44581 cgatgcttat cagcaagcaa acagcttttt acgaggaaaa gattatctaa aatggctgcc
    44641 aaagaaagaa aaacatacga aacaaaaaaa caaagaagat atccatgcgc attatgactt
    44701 aggtaacgac ttttataaac tttggctaga tccaactttg acttattctt gtgcttattt
    44761 caatacacct gaagatacgt tggaacaagc acaaatcaat aaagtccatc atatcttaga
    44821 caaactcttt atcaaagaag gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt
    44881 gatgttcact gctaccaaag agtataatgt caaggcaact gggatcacac taagtgaaga
    44941 acaatacgat ttgattacca aaagaataaa agaagaacat ttagaagata aatgccgtgt
    45001 attattaatg gattaccgcg aactaaaagg cgaaacgttt gatcatatca caagtgtagg
    45061 tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt
    45121 attgaaacca aaaggaacag ctttgatcca tggaatcagc cgacaacaag gcggtgcgac
    45181 aaatgcttgg atcaataaat atattttccc tggcggttat gtccctggtg ttactgaatt
    45241 agttgataat attacaaaaa acgatttgca actgattgat ttggaaagct tacgtcgtga
    45301 ctaccaattg acattagaac attggacaaa gaattttcat caagtccaag atcaagtcac
    45361 gaaagaaatg ggtgattcct tctatcgaat gtgggatcta tatttacaag cttgtgcagc
    45421 ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa
    45481 tgacatccca atgcatcgtg cataattgag gcaaaagtac tgtttaaaga ctatttaaaa
    45541 agaagctaag ttttatgatg ctccagaagt gaactttttt gtctaacttc taggattgtc
    45601 tctaaagctt agtttctttt tctttatatt ttcataataa aagtttggtt ctttaatttt
    45661 taaacatgac agaaaatttt ttcttcgtta tacttgtaat agaatactat tttataacaa
    45721 aggggatcga atcaatgaaa aaaactgtta tttatactag tttatttgca gctttgtttt
    45781 tagctgcttg tcaatcaaat accaatcaag gcacatctgg gaatacgtct tccagtagcg
    45841 aacagcgctc ttcacaggta tcggactcct caacaaaagc aagcacatct tcgtctagcc
    45901 agacaaaaag ttctagtgaa gcaagtactc agtcagatat caaagtgtct gcagctcgtg
    45961 cgatcgaatt gttccaagat aaatacccag atactgcaat tactagtttg gaattagata
    46021 gtgattgggg atcttacttc tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag
    46081 caagaattga tgctcaaaat gaaaaaatgg agacagagaa tccagaaact ctagatcgag
    46141 atgaacaaaa tggtcaaaag aaaaaagaag atggtctgga tgttgataaa ttgatcacga
    46201 ttgaggaagc agcaaaaatc gccgtcgaca aagtaggaag cggtacggca acagattggg
    46261 atttggataa agatttaggt actacttatt gggaagtcaa agtaaaagac ggcaatcaaa
    46321 gtacatcagt caagatcaat agccaaacag gcgaagtact gtcaactgaa caagatgatt
    46381 aagaatttct tcggaaaaca agagttgatt tttcttttag tcatgataca ctagacaaaa
    46441 taacttgttt gaaggagcga tttacttgag tttagaaacg acctttgcca atcatatctt
    46501 tgccaatcct ttgatgaatg catctggtgt ccactgcatg actacccagg aattagatga
    46561 actggctcat tcagaagcag gagcatttat cactaagagc tgtacgatca atgaacgaaa
    46621 aggaaatccc gaaccacgct attttgatgt tcctttaggg agtatcaatt ctatgggact
    46681 tccaaactta ggtttttctt attatctaga gtatgctctt gcgtatgaaa aagtgcagga
    46741 aaaccaaaat caaccgttat ttttttccat tgccgggatg agtgtccagg aaaacttgga
    46801 aatgcttgag aaaatagaaa aaagtgggtt taatgggatc actgaattaa atttatcttg
    46861 tcccaatgtg cctggaaaac cacaactagc ttacgatttt gaggccactt atgaaacact
    46921 gaaagaagta ttcagcatct tttctaagcc gctaggaatc aaattgcctc cttattttga
    46981 ttttgctcat tttgatcaga tggctgatat cttgaatcag tttccattga cctatgtcaa
    47041 tgctatcaac agtgtgggca atggtttgta tatcgataca gaacaagaag cagtagtcat
    47101 caaaccaaaa gaagggttcg gcggcattgg cggagagtat atcaagccga ctgctttggc
    47161 gaatgttcgc gctttttata cacgtttgaa accagaaatc caaatcattg gtacaggtgg
    47221 gattcggaca ggacaagatg catttgaaca tctgctatgt ggcgcaagca tgttacagat
    47281 tggtaccgag cttcataaag aagggccgga aattttttct agaatcataa aagaattaac
    47341 gcagattatg tcagaaaaag gatatacgtc aattgacgaa tttaaaggca aattacgcac
    47401 catttcatag caaagagacg aaggtcatga ttgatcttcg tcttttttta taaaaaacac
    47461 tcgtctgttc attcctgtgc aagcgtactc attgtatgtt agacgaactt gtgggaaaat
    47521 aacagaggca aaaaggagga taatatgaat gatgcaacag aattagcaaa agagcttaaa
    47581 gaaaaagaat ttagttttca agaactagtt acggatatac agaaaaaaat aaagaagcaa
    47641 aatcatgaat tgaatgcgtt tgtcgcgctt gaaccattcg agagtaattc ccacgagcta
    47701 ttgattcccc agtcagaaaa agggccattg gctggtatcc catttccttt aaaaatgctc
    47761 ggtcaagaaa aaaagggatg gctagctact tcaggatctc gtttatttga aacacaccga
    47821 gcttctcata catctaatta tgtgcggcaa gcagaagcga taggtctagt tccttttgga
    47881 caaaccaatg cgccagagtt tggtttcaaa aatattacag acccagtcat ctatggccca
    47941 gcaagaaacc cttggaacct tgaatgtaca ccgggcggat caagtggagg agctgctgct
    48001 gcagtttcat caggaatcgt tcctttagct ggtgctagtg atggcggtgg ttctattaga
    48061 atacccgctt cgttttgcgg attgattggg ctgaaacctt cgcgagggac gatgccagtt
    48121 ggtccatatg cttggagagg atggcaagga gctgctattg atttcggctt gacagtttct
    48181 atgagagaca cggaggcttt attttatggg atgagaagta tccattccgg tgcgccatat
    48241 caagtatcgc cagttgagtg gcaaacacat ccaagaaaaa aacggttgaa gatagctttt
    48301 tgtgttgatt caccgatcca ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa
    48361 gctgtagatt tccttggaaa agaagggcat gatttgacag agattcctta cccactagat
    48421 ggtcgtaaac tgatcacgtc ttattatcag atgaatggcg cagaaacagc agcaatgatc
    48481 cgctcgattg aaacaggcat ccagcgagct gtcagaaaag aagaacttga acccatttct
    48541 tggactttgt tccaatatgg tcagaagctc tctgccgcta cttacatcga ttctctgcat
    48601 gtatgggatg aagcagcaat tgtcatggaa aacctacatc aagaatacga tctattcctt
    48661 tcaccaacga ctgcatttac cgcccctaaa atcagcgaag atttgcaaag tgatgcgata
    48721 agagcacaga tggaacacat cagtgattgt tctgaagtag aaggtttaga cgttgtttct
    48781 tccatgtttg aggagagctt gaagatcaca ccttatacac agttggctaa tctgactgga
    48841 caaccagcaa tcagcttgcc tactcatttg acaaaagaaa acatgcctct aggaatccaa
    48901 tttatggctg ccagaggaag agaagatttg ctatttcaaa tcggctatct atttgaagca
    48961 aatcatcagt tccatttacc aaatccatgg aaatagcaga aacaaggaga agaagaatga
    49021 agaaaaaaag attgtttgct ctaataacta gtgccttttt tattttaagc gcttgcggta
    49081 cagctgccgc taatcaggaa gataacagct ggagtaatgt agaaaaaaag aaagaattag
    49141 tagtagcgac ttctggcaca ctgtttcccg catcttatta taatgatcaa aatgaattag
    49201 ttggttatga tgtagatttg gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct
    49261 ttaaagaata caacgtggat ggacaaatca cttctgtttc aaaaagagaa tcagattttg
    49321 ctgcgaacga ttttggtatc acaaaagaaa gagaaaaaaa attctccctt tcagagactt
    49381 tcaaatattc atttgacagt atgatcgtac gaaaaaaaga tgattcagga atccactcac
    49441 ttgaagatct taaagggaaa aaagcagctg gcgaacccaa tactagctat atgcgtttag
    49501 cggaaaaaca cggagctaaa ttagtcacat atgacaatgc cacgaatgat cagtatttaa
    49561 gcgatgtagc aaatggtcga acagatgtta ttctaaatga ttattatctg cagaaaatgg
    49621 ccgtatctgc tttaccagat atccctgtaa aaatactgga gaacgtttat ttcaatgaaa
    49681 acgaaagtgg ttttttattg aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg
    49741 tattaaaaga gatgaaagaa gatgggacga caaagaaaat atctgaaaaa tattttcaaa
    49801 ctgatgtttc agtcaaaccg aaagaaacga tggctgagtc ggtgagtgag tgatgtactt
    49861 tcctctaatt gatttttccc tcatgtggac ttcacttcct ttcgtactgc aaggcttggt
    49921 atacacatta gggattggct tcgtgtcgtt tcttttagga aatctggtag ggttgctttt
    49981 gacagtttta ggtcttttgg attggctgcc tctgaatgta tttattcgtt tttatttatc
    50041 gttctttaga ggcattcccg cactagtcct gctgtttctg ttatattttg ggttacctta
    50101 tcaattaagt gccttaacgg cttcagtcat ctgctttacg attaccagca gtgcatttat
    50161 tggtgaaatc tatcgaggat cactagcagg cgtttcttca ggtcagtggg acgctggttt
    50221 agcttctggt ttttctttct ttggggtatt gaaatatatc gttttgccgc aagcttttcg
    50281 aatctcagta ccggcactca gtaacgtagc tatggatcta gtgaaaggta cttcgttagc
    50341 agcaacaatc acagtgccag aaatatttca aaaagcaaag atcataggcg gtcggacatt
    50401 cgattatttt tctctctata ttttagttgc actgatttat tgggtcattt gtatcgggat
    50461 cgaaaaagtg caacagtatt tagaaaaacg ttctatgcat cagtcaatct gaaaaataga
    50521 aatggtgcta gcgtggctgg caccatttct attttttgtt tcttgtatat tctgcctagt
    50581 attttaaaac catttcatgt aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg
    50641 aagaccccgc taaccatcga aatagaatcc tcgttatacc aatattgcat cgagcaaggc
    50701 ggcttggttg tagaagaagt cacgatgcct gatgaccaag gaattgtaga tacgcttagc
    50761 tgcttgacaa aatttgatgg aagccgagaa tggcgatgct atgagctgaa agtgacaaaa
    50821 gcagattttc gctctacagc caaactgtca tttgttggac attataatta ttttgtttta
    50881 ccaagtaaac tatatgaaga agtaaaaact gaaatcccta caggaatagg tgttttagtg
    50941 tatcgtccgt acacggttga agaggagatg cctgcctctg gtacatttct gattgcgaaa
    51001 aaagcagcaa agaaagagct tgctgtaccg gaagaagctt tgacaaaccg cttcatggct
    51061 tctttattca gggaagtaag aaaagcaaag caaatggagc gaggcgtaaa atattttcca
    51121 acagatcaat tgtacaaaga attgaaaaga cgttcagaaa atcaagatcc attttctaac
    51181 gaacgatatt atgaacggtt tttagaggaa atagctagtc aggcaatcaa tgagttgcaa
    51241 gaagaagtta tcgcgcttca gcatgattat gaatatttga ggcaacaaca gcagatccgt
    51301 cggttgccta cagagccatt ggagtgaaaa atatgtacta tccttattta agaggaaaac
    51361 aatttgacct attagcatta aaagaagcac tgtcgcgagg attgctctcc aataaaatcc
    51421 aaccagtgat cgaaccagta cgtgattccg ctacgcttaa aaatgtgatc gaattatttc
    51481 aaaaaaagca ccatcgaatc gctgtgatcc aaaatcccca agtaggacaa ttcaaattat
    51541 tcgatcagca tgtgcacagt tggacagtca aagaaaatag ctctgtcgtc cctgcacaga
    51601 tcttaacgcc agaaaatctc agtgaagtac tggattcacc tccagcgttt cttgttttcg
    51661 atggacagca ctacccaaaa gatacagagg tgtggaaaca gttggcagga gttgattcga
    51721 aatttttgat accagatact agtcgctttc gcatctggct gcccgaaaac aaaattgtga
    51781 tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta tgcagataaa acagatgact
    51841 tttttagtga tgactatctt tttttccacg cagacggata tattggattt tctgatttca
    51901 cgatcgaagg tagccgttat ttcgataaag ggttccctag ccgagcgctt gctatccatc
    51961 taacgtatat tgatgcttac ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg
    52021 actctgctaa agaccaagca ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga
    52081 tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat gattgaatta ttggctcttt
    52141 accagcaaca aaaattccct ggactgggag tgttgaaaaa gtggtcactt atgcatcatt
    52201 tggaacttat tagtcacctg ctagatcatc caaaagattg gcttaaatat tattcaaaag
    52261 ttcaggaact atatgaagtg atccaatgat tagataatgt aaatgaaaaa ggaatagaaa
    52321 agaggaaact ggatgtatat tcggcaaaca aggaaagaag aagtagaaga agtggtagaa
    52381 atcattaatg ccgcagcgga tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg
    52441 cagaaaccaa caaaagaaac gattttgaac gatattgaaa gaaaagaaag ctatgtatta
    52501 atagtggaag atcaaattgc tggaacagct gcattagtct caagcgtgga tcccgtttac
    52561 acaaaaatag atggtagttg gaaagaatca actggtcctt atttatcgat tcatcgtgtc
    52621 gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg
    52681 acgattgcag ccagcaaaaa tatccaagat atccgtatcg atacttatcc agctaatgaa
    52741 ccgatggaaa agaccatcta ttctgctgga ttcacttatc aagggatgat tgaatttcct
    52801 tttgaacacg gcgaaagaaa agcatatcaa ataatcatca attgactata aaatcatctg
    52861 agcaaagata aagacattaa cactactgaa aaaaacaatc atatgatagc caagaggttg
    52921 taacagaagt ggccagcttc aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt
    52981 ttgtggaatt ttagcgtatt ttacgaaata gctgctttct gaaactccct tcatcgagag
    53041 gtcgtgacat agcttttgtc acgacctctc ggcttatcct acgagtatta ttcgatacat
    53101 ttatatcaat aatccttttt ttattttatc tgcagcttct tggaacgctc catcgtttga
    53161 cacgactaaa agtgagggga tagctttcaa acgtttcaac tgtgctaaat cttctacata
    53221 aaccgccagt ctggctgaaa tctgttcaga tgaatctcca cgttttttca ggcgcgattg
    53281 caatgtttct ttagagatcg taataaagat aggacggact accgtcccga aatactcata
    53341 taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt
    53401 tttaatttct gctttactgg aaccatattt atgttgagca tattggtccc actctgcaaa
    53461 ctcatttccc ttgatcatct gattgaatgt attgtcagaa acaaaataat aatcctcttg
    53521 atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta atgatctttt gttccggctg
    53581 aaaaatagct tctgcgagtg aagtttttcc agaaccagaa ggaccgataa atacatagca
    53641 ataattcatt gtcatttctc ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt
    53701 tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta taccagcaag ccagtcacaa
    53761 tagcacttaa aaagctgact aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga
    53821 caagatttcc tttttcttca tttaatccct tcatcgcacc agaaatgatc ccgatagaag
    53881 agaagttggc aaatgaaacg aggaagacag atataattgc tgttgtacgc tcagtaaaat
    53941 ggaaattacc atttgctaat tctgtcatag caacaaattc atttgtaacc agtttagtcg
    54001 ccatgatact accggcatcc actgcttctt tccatgggat tccgctgatg aaggcaagag
    54061 gggcaaagat gtaacctagg atctcttgga aagtgatgcc gaatatcccg tggaagatag
    54121 cattgatcat ggcaatcaaa gcaacaaaac cgataagcat agcagcaacc gtgatcgcca
    54181 catggaaacc atcaagaatg tattctccca acatttggaa aaatgtctgt tctttgtttt
    54241 cttcaatgat cagctcatct tctttttctt ctaatacata gggatttaca attgaagcca
    54301 gaatataacc gccaaataag ttcaaaacaa gggcagtgat cacatattta gaatcgatca
    54361 gtgccatata agatccgacg atcgacatag aaactgtaga catagccgat acactcatgg
    54421 tgaacaaacg tttctcagat aggaagggta attccttttt tatggaaata aaaacttccg
    54481 attgtcctaa aatagcagaa gctactccat tataagattc caatttgccc atcccgttga
    54541 ttttacttaa tcctagacca acagctttca taaataatgg aagaatcttg atatagcgta
    54601 gaataccgat aatagcagaa ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg
    54661 cacttccttt atttgctaca ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa
    54721 gaagatgatc aaacattttt gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta
    54781 ataatacgaa accaagaaca agctgtaaaa cgaacatcac aattagtggt ttcaatttga
    54841 ttgctttttt gttactgctg atcaaaaaag caagccctaa tgtgacaaat aaaccaataa
    54901 tacctaataa ataactcaaa taatcactcc tattccaaat tccgttttat tttacctgtt
    54961 ttgcaaataa aatactaatc tttagtgaaa ttttcattac ttttttagag tgaaacgcat
    55021 tcattttcca ttagaaaata aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta
    55081 gtaaagtcta taaaaaaacg aaactatcga ttaagctaat agtttcgttt tcaacattta
    55141 taagtaaact ttttctattg gtaatataga ttttcggaag ggtagacagg tgcgcaggta
    55201 atatcgattc ctagtttttt caatgtttgt tcatcatttt tattgaccat cacagtagag
    55261 tgggcttgtg ctccctcaag ttcagctagt ttatcataag caacttgagc gattggattc
    55321 gttactgcac tgattgataa agcgatcaag acttcattga tgcttagtgc atccaaagag
    55381 gaatgaagtg tctttgattt caatccttgt attgtttcaa gaactaatgg tgacaataaa
    55441 ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat ttaagataac tgccgctgac
    55501 gcatccatca atgaacttgt acgcccggta atgatgactt catctggtaa ttcgattgca
    55561 atcacagcag aaggatcttt tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg
    55621 gcagggacta ccacgtttcg gtcttcagga cgtaaaccga cttcttccat aatcagtttg
    55681 attcgattaa ctgcttcttc gtcgatcaat ccttttttga agtcgcattc tgtttggaaa
    55741 taacgacgga tgatttcttg tttagaggct tcttggacaa cggtatcatc cacgatccca
    55801 aatcctacac gattcacacc catgtcagta ggagattgat aaacagattc tttaccagta
    55861 attttttcaa taatacgttt gattacagga aatgtctcaa catcccgatt atagttcaca
    55921 gctactttat tgtaggcatc aaagtggaag gaatcgatca tattgacatc ttttagatcg
    55981 actgtggctg cttcataagc aatgttcagc ggatgtttca acggaacatt ccatactggg
    56041 aaagtttcga acttggagta accagctgca tgtccttttt ggctttcatg atacagttgg
    56101 ttcaaggaag tagcgagctt tccacttcct gggccaggag ctgtcactac aacgattggt
    56161 ttagttgttt caatatactc attttttcca aaaccatctt ctgagacgat tttttctacg
    56221 ttgactggat agtcttcgat ttctgagtgt ttatagacct tgatgtttcg tctttctagt
    56281 ttattgataa agactttcgt ggcaggctgt ccactatagc gagtgataac gacactgttg
    56341 attaataagc cataaccacg taattcatcg attaaccgca agatatccat atcataagta
    56401 atcccataat caccacggat cttattacgt tcaatatctc cagcatagac gcaaatcagt
    56461 atttcagctt gatctttcaa tttttgcagc aatttgattt tcgcatcttc ttcaaatcct
    56521 ggaagtacac gtttcgcatg cttgtcgtca actagcttcc caccaaattc taaatataat
    56581 ttgtcatagt ggttcactcg ttccaaaata tacgctgact gctcttcaat atatttttct
    56641 gaatcaaaac caattttttt cactaggttc cctccttaag tactaaagca ttttttcatt
    56701 tttttgagga ttcgtcaact ggaagagcga aattcctcaa aaaaacaaag aaaatggttt
    56761 aaaccaactt gtctagatag ttattcattg gtatgatagt ttttgataag gaaggtgcta
    56821 catgaaaaaa agacagaaaa agaaaaacgc atataaacac tatatccgtt cgatttttac
    56881 cggttatgaa agaatgcttg aagatcccga attggaacaa ttgacgttta cttatctaaa
    56941 cgaagaaacg cagctaactc gtgatgagca tcagcgcatt catttcacaa caagagatct
    57001 gcctagtaaa taaaaaataa gataaaggtc atcggattcg attctgtcct atcagatgga
    57061 aagactagtt gatcgaaaaa aaggctgtac caaattttgg tgcagcctgt tttatggagc
    57121 attgcccgct cttttttata acaatttttt acagaaaatg gtactattga agtattaata
    57181 agtgaaaaac agatgagtaa gaggaggcaa gtagtatgaa agagccagat acaaatgcaa
    57241 attatgagcc agacgaacta gaggagacga atgcggatca ggatacagga caagaatcgt
    57301 cttcttttta cgatatcaaa gaaaaagcaa gcatgaatct ttcccaatta ttagaagact
    57361 tacaggcttt tgaaaaagct gtccgcgaag aagatatccc tgagatctac cgtttataca
    57421 atggccgatt gaatgaagaa ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat
    57481 tgctgatgcg taagatccat gatcgttttt tacaggaatt tccttttatg gaacaagtag
    57541 aaaacatctc gccaacgatg agctattaca aaatcggcac ttattaccat gatcggccca
    57601 cggtcggtat tgatgcaagt ctgccagaaa tttttgtttt gcctaagatc gatgaagagt
    57661 gggagaaata ttcccaaccg gagtcaacgg actatgacaa aaaaatcaat gagctagacg
    57721 caaaggtgat cactgctcag acagaaattg aacgtttaga tgaacaaata aaagaaatca
    57781 atcgacaaat gactgatttg aacgacaata aaggattctt gaatcgtaaa aaaatcgagg
    57841 aagaaattca agaattagag aagaagaagc aagtactatc taatgaaaaa ttaggctggc
    57901 ttccttatat tgagacgcct gaaacaatcc agcagcaaaa agaagcattg aagcaagaag
    57961 caagagcaga ccagcttcgt gcagccatcg tagaaaaaga acaaaggcaa atcagacgct
    58021 atttcggtag caaagaggga ttcggtcaag caatccatga attcttgatg aattatcttg
    58081 gcaatgagaa tcctaataat caatctaatg aaggaggttc cgagtatgaa tgaacacgat
    58141 gaagcagaat ggacaaaaaa tgaagaacaa acagaagatc atgaagaaac gaacacttca
    58201 ttggaagaac aaacaagtaa tcctctggat gacacagaag aaatccctat gctagaggaa
    58261 acaaaaatac acgaaaaaga aaaagtaact ccgacgaata cgaatactgc agaaaacacc
    58321 catgcttacg aaataactga aaatcccggg tatttagaag gaaaagagca agatcatcaa
    58381 gatatcgtca gtttgattga aacagcggaa gcagaattag cggatttacg tatgagaaaa
    58441 gatttgatcg aatcagattt tccacaatta ttagaatcat atgatcgttt tatattgaat
    58501 gaagaaccaa tctccacgat cggaaagaca agcctgatcg aatacttcac gtatgaatta
    58561 ctgaaaccgt tgaatgatat tggtctggaa cggtcaaatt cagcttatga cacatggaaa
    58621 acaagacatt tttcgatcaa ttatacgttg aatggacaaa acgaattagt cttttccttt
    58681 gtattaccga ccatcgatca acgataccaa gtggaatcga tgagtctcct aaacgtatca
    58741 ccagagacaa tggaaatcaa tgtccaagat gaccgggtgc tttctttgat ccgacactgg
    58801 tcagtggatc gagtattctc tgcagggcag atcacgatat tcaatcacaa attgaatcaa
    58861 ttattagccc atgcaagaac acttggtttt accgttaatc agacattact agacaatacg
    58921 aagccacttc gtttgaatct tcagagcgaa tttgaattga cgaatgaagt attggatgat
    58981 atttttatcg tggcaatgaa aaatccatcc tacgatatgg aaaagatcaa cgaaactact
    59041 tatcaagtac gattagataa aggacagtct ttaacgataa caacaaacga aaaagatcaa
    59101 acagtcttgt cgatctcatc tggagactac ccacgatctg tcattgattt ctttatcaat
    59161 tacgaattct tggtcccatt aatggtgaga aagtcctgat tggataagtt ggattaccag
    59221 atgtcattca gataattctg ttccaaagga cttgcggcag ttatccagaa taattgtcac
    59281 aagtcttttc tgtatgtact aaatggacgt gaataacttg ctgatgaagg agggatttca
    59341 ttaggaatgt ccacaaataa aatagcacag cttagcttat tgtctgctgc ttgtgtagca
    59401 ggtaggatcg cttttcaatt tattccgaat ttccagccaa tgactgctat ttttttattt
    59461 atagtacttt acttaagctt aaaagatgcg ttaattgtca tgagtctttc tattgcgatc
    59521 agcagttttt attttggtgt tggaccatgg gtcatcggcc aatggatcag ctatactgtt
    59581 gttctgagtt tgttttcaat cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta
    59641 aagggagtct gtttttttct ggcgggcatg gtttacggaa taggtatgac cgtttttgat
    59701 acgctgttat atcgtttgcc tcaaccttgg atctattacc ttcaaggggt gtcgtttgat
    59761 ttgatgcaca gtataggcaa tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt
    59821 ttttacaatc attcaggagg aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag
    59881 tgtcagtata ctcattttga caagttgcag tacaacctat acacaatcgg cttccaacca
    59941 aaaggaggaa gtaaaaacat cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa
    60001 cttttcggaa aaagaagtgt tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg
    60061 aaattttgaa gtcaaagaag ataacggctt catcacttcg cttgaagggc actcacagga
    60121 tgagaaaaat caactctatt ggatgtttac tgtggacggg aaaagtgcta ctactggtgc
    60181 caaagagatc aaattgaatg atggacaatc ggtcattttc aatttgtcta aactataagt
    60241 ttcataaaaa actgttaaag ggcgaatatt gccataattc agtgagttca gttgatgaaa
    60301 gaaggaaaat aacatggaaa attttaaaaa aagcggaatg ttttccgtaa ttgctgctct
    60361 aggggctaat attctagttg ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc
    60421 cgccatgcta aacgaaagca tccacagtat cgttgattgc agcaatcaga ttttgctgtt
    60481 attcggtgat aaacaagcca ataaaggaca gagcgagttg catcaatttg gtgaaggacg
    60541 agcaaaatac tttttcagta caatcgtcgc aatgatgctt ttcttcggcg gtggggcact
    60601 tggggtgatg gaagcttttg aaaagttgac gcatccatcc catgaagtgg gtaatccgat
    60661 cattgttata gcaatactgt tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc
    60721 aatgaaagaa atcaaagaat tgaatactga aaaactttcc acgatgcgct ttttgcgcga
    60781 aagtcgacac agtgaaatac tgattatttt tacggaagac ttgtgtgctg taatcggttt
    60841 ggtcttggct ttagcaggaa caatattgac ttatgcaacc gggattgctg catttgatgc
    60901 gatcagcgga ttgctgatcg gtttcttgct gatgggagcc gccattttcc tagcaaaaga
    60961 attttatagt ctgatcatcg gtgaaagcgt gacagcaagc gatttatcaa aaatcaagtt
    61021 agcttttgaa cgtcctgaga tcagtcggct gatcgatata aaaacagttc atttaagtcc
    61081 aacggagatt ttagttgcag caaaagcaga tatcgttggt aacaaagaga atgaagctta
    61141 ctcggtcatc aatgatattg aaaaaaatat gcgtcagtct cttcctgaca agaagatgta
    61201 tatatacatt gaaacagata aatatgaccc aaattattct cgtaaataaa acgtttattc
    61261 atgaattgaa gcaaaaatga gaaaaatata atcttgtttt taaataaaac ggctacaatt
    61321 tagcgaattt atgttacaat gagtccgctg tttatttgtt gtaattaaag gagatatttt
    61381 aatgaacaaa aagaaaagtg cgccatggct tcaagctttg catgcagtga tgccactttg
    61441 tataagctat ataccagtag gattagcttg cggagtattg ttgcaaaaag ttggatttga
    61501 tcctctctta tcaggactgt tatcgattct ggtcttttcc ggtggcgcgc agtttttagt
    61561 tgcatctatg cttacgacgc aagcatcatt tgcaacgaca ttattgatgg ttttcttttt
    61621 agaattacgc tatacattat taggagcaag tcttgccggt tttatgaaaa aagaaaagcg
    61681 gacgtttttg gctgtctttt ctcaatcact gaatgatgaa aactatgcgg tgaactactt
    61741 aaagttttca actgaccctt cctggaataa aagaaaggct ttatatgtca attggttctc
    61801 aatgactgca tgggccggta gcaatatgat cggtaatttt ttcggctcgg tgattcgagt
    61861 agatgctgac cttgtccatt ttgctttgac ggccatgttc attttcatgt ttatcatgca
    61921 gatgaaaagc gcactactga tctttacagg tttattctca ggcgtattag gggtcgtatt
    61981 catggtatta ttccaaaata ctttcggatt gattgcagca acagtcattg cttcatttgc
    62041 cggatttatg ctggagaagg cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg
    62101 taaagatgtc aatgagccgc tcttcaactt cccggaacag gaggccaatc accatgacta
    62161 aactatattt gctactttta gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt
    62221 tcccgatgct ttattttact catcgaaaag tgccatcctg gttcagtgac tggatgaaat
    62281 atgtaccggt ggcattattt gcggcgttag cttttaaaga tgttttcatt actcatgaac
    62341 atctggatat tgcttggaat atcaaaatag ctgcaatgat tctagtagca ggcgtggctt
    62401 acaaaacacg ttcgatggcg ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt
    62461 ctatgcttta aactgtttta ttttattaaa aacagagatt gcgacataag ttgttcaaac
    62521 tgatctatcc gaactgcatt tgcaacagtc aattctggat gcaagtttat tcgtagggat
    62581 aaaaaagatt agtgttggct tatgatgcaa gctctttttt attttaggaa ttagagaggg
    62641 atttgtcctt ctatgatttt ttgctataat agggaagatg tttattttat gaaagaatag
    62701 ttgagggaat ttaaatgaat aaaaaattga tgcaaggaac attttggcta acctttgcta
    62761 atcttctatg taaagtttta ggggtagtct atttgattcc ctggctaagt atgatgggga
    62821 acaatcaaga tggtatgctt gctacgacat tgtataatgt tggctatctt ccttatggat
    62881 tgttcctgat gcttggtaca gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag
    62941 ccacaaaaga aggagataac gctgcttgtc ggctgatttt tagaagtacg atcaatatca
    63001 tgtttgtcat cggtatcgcc tctgctgcac tgatgtattt atttgctcct ttactagccg
    63061 gaatcagtcc gattgctaac gttaataatg ggatactagc tatcagaagt ttatgcccat
    63121 ctttgattgc tatcccaatt cttagtgcaa tgagaggata tttccaagga aaaaatgatc
    63181 tgcggcctta tggaacttct ttgattatcg aacaggctgt gcgagtgatc gttatcttgg
    63241 ctggtactta ttacttgcgc gtattgactg acggaaccat attagaagct gtattgatca
    63301 gtacagtcgc ttctttcttc ggtgggttag ctgcgattat ccatatgtat atcgtcggac
    63361 gcagaaaaga cttcttcgaa cttaaagatt ttttgatttc cagccgttat ttcgaaagag
    63421 aaaaccgatc tgcctctgtc tctatcattc gggaaacact accgtttatt tttgtcggct
    63481 cagtcattac tatcgttcaa atgatcgacc aagtaacgat gaaaccactg cttcactttt
    63541 tccgtccaga gatcgcagat cagcaactgg aattcttatt cagccgtgct tctgtcaatc
    63601 caaataaatt gacattgatc ctgatctcca tggtaggaac agtagctata agcagcttgc
    63661 caatattaag cacgatgaag aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata
    63721 gtttttcgat tgcgttattg attcttcttc cttcattgac tggaatgtct ttattagctg
    63781 gaccactata cacattgttt tttggctatg atcctgaaag tgtcggatat ttccaaatgg
    63841 ctttgctagc ttctttgttt ttctctttat tcacgatcct atctacgatg cttcaatctt
    63901 tgaatcacca tcgttttgcg atccgattaa ctgtagaagc aattatcctg aaagtcatct
    63961 tccaagtaat cggattagga ttagttggcg gatacgggat gagcttatca aatggcttag
    64021 cattcggtat tgtctttgtt cgcggctatc actttatgtg caaagaatac cgaatagcac
    64081 ctttagcaaa gatcagcaat tttttcttaa aaactttccg ctccaccctg attatgcttg
    64141 cagtatgttt tgccgttttc tttttgctga atcagcgatt gtcaatggaa tcaaaaagct
    64201 atgcaatgat ttattgtgtg gctgtaggaa gtattggcgg cttagtattt ttattttctc
    64261 aattcggaaa aaatgggttt cgaatgttga aaaatgttaa acatcatagc agaagcaaag
    64321 aataggttaa ctaatcgttt tttgctgtca cttgaaaaac ggcaactcgt atgattacgc
    64381 ttgtcgtttt tctattttcc aattttagtt tcatatctaa tcttacttat gatatagtta
    64441 taaggtctgg aaaaattctt tgaaacgagg aaattaaaca catgaaactt gcagtagtta
    64501 cagatagttc ggcatatctg ccggaacgta tcaagaacca tcccgactta ttcgttattc
    64561 caatcccttt gattatggat gggaaaattt ataacgaagg aatcgatatc gaagcggatg
    64621 aatattatgg acttttaaaa aacagtaaag attttcctac tacttcacaa cctgtcgtag
    64681 gggaagtttt gggtttatat gaggaattga aagagaaagg gtatgacacg attttaagta
    64741 tccatctttc ttcagggatt tctggctttg tcaacacatt atttgcgatg aaagatgaca
    64801 tcagcggagt aacggtcatt ccttatgact ctaagatcac tagtatgccg atgggacaca
    64861 tggtagaagc tgccttagac ttaaatgatg aaggtcagtc ccttgatgaa atacttgcac
    64921 atatcgatcg aatccgagat aatacctacg cttatctgat tgtagatgat ttgaacaatt
    64981 tagtacgagg aggacgtttg acaaatggtg cagcgctgat tggcagctta ttaaaaatca
    65041 aaccgatcct gacattttct gaagggaaaa ttgttttatt cgaaaaaatc cgttcaagta
    65101 aaaaagcatt tgctcgagct gaagaaatta ttggtagaag aaataacgaa atacaaccgc
    65161 cagtcaaact ttacgtgata catgcaaata atttagaagt agcgattgcg gagaaagaga
    65221 aattgcagaa aaaatatcca aatgcagaaa tcgaaattgg tcatttcgga ccagtcattg
    65281 gtacacattt aggtgaaaaa gccatcggca ttgccatttc agcgcaataa ttccttttat
    65341 tcaaaaaaca taccaaaaat attccaacat catcttggac tttctacatg ttactaatga
    65401 gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaat gaagctcttt ttctttgcaa
    65461 tcctcacttc aatcctctat aatgtcgcat atagtttagt aaatgagtaa actactaatt
    65521 tacttattta ctaaaaataa aaggatgagg agttaaaata atggaaatac caacaaattt
    65581 aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt
    65641 aaatacggat gcaaaagggc tttcactggg ccttgctcag tggaatgatc gtggaaaagt
    65701 cgatatctca ggcaaggtat ggcgacatac tggagaaaaa tggtcacgac agtcggaaga
    65761 attaccaatc acacgtattt tagatttagc gatcttagct gcacaaggaa gtttatactt
    65821 ccaagatgct tatcgttatg aaaaatttta tgatccagaa aatccagtcg tagacatcat
    65881 tggattacaa gggaaccgaa tgacggtaga agtagatacc aaaaatccaa agatcgatga
    65941 agacatcatt ttgtattacg atacactaca aaaagacgga gaactgattg gggaacgatt
    66001 ccgtatttta aaacgtcttt tagatgagct aggatattaa tagacagaga tgatcaaaag
    66061 gggaagagaa gcatggagaa tttctcacta acagaaaacc gaaccttttc acggctagaa
    66121 aagcaaatga tctggaagaa accgaaaagc catcggacaa gtgcagaaga atggcggatc
    66181 gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga aaatcagtaa cgtacttttg
    66241 gaaggagatg ccgggtcggg gaagacacaa ttagccaaag cactatctgc tgatctgcaa
    66301 cttccctata caaaagtgac ctgttttgca gatatggaca aatcagatgt tttcggagcc
    66361 ttattacctg tagtagattc taataaggaa caagaccaag aattactaga agctatttac
    66421 caaacagata cacttgaagc tgtactgaca ctgatccaga accattacgg cattgatcct
    66481 ttttctgcta aagaaaaatt agcagagcta gttcaaagga tcgaagctga ttatccagat
    66541 gcaatccatt acagctatta cccttctgaa atcgttcgag cgattgaaaa aggatattta
    66601 ttagagattc aagagccgac tgtcatccga gatgcctctg ttcttgtggc acttaactca
    66661 gcgctcgaac caaatgggat gttgaattta ccgacaggaa tcattcgtcg ccatccagat
    66721 tgcgtcattg tgatcactac aaatcgaaat taccaaggaa atcgcccatt aaatgaatct
    66781 ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc ctgccttaga agtcatggca
    66841 gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac ttctcttaaa gatggcagag
    66901 attatccgtt tgttggatga aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg
    66961 cgttcttatt tttattgggc aaatacctat aagcaaggac aagatttact ccagtcgatt
    67021 tatccaaaag ttttgtataa attatccact gattcagatg aattggcaat tcttgaacaa
    67081 gctttgtctg acagtggatt gctactggaa cttcaggaca tattgagaaa agaaaaatgg
    67141 ggtcatcctc aatctgagca tacaaaagga agaaccatta gcgccgaaga agcaaatgaa
    67201 cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct
    67261 gctgaagaac caaatgaggg agaagagaaa caagaagaaa caatagaaaa aaacgagatt
    67321 cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa
    67381 caaacaggag atgattctca agaaatgtct gcttctgaca tggaagcaca tgataaggaa
    67441 atcaagaagc aactgaataa agaagcgcga caaatcatga aggaaacgat tcatgaaaaa
    67501 gaaggtctca tcgttcaccg accagctttc caaggaaaaa cgccggaagc acgtgagcta
    67561 cacatgcaag taatgccaat agtagaatca cttagccggc aaattttaga gttactggat
    67621 aatgaacaaa cagagacgta ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc
    67681 aaagtagcat atggtgattt gagaaatttt gacaaaaaga acccgccaca tgagcaacct
    67741 tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa tgatacggga tgatcggatc
    67801 gaagcggcaa aaaaagcagc gattgccatt gccgaatttt ctaagaaagt cgatatccca
    67861 ttgttgattt acggagatac agcagaccgt tcgtcaagag aaaagacttc tttgttttct
    67921 tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa aattagtcac tatgaaacca
    67981 aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag cagaaaaatt aaacagacaa
    68041 tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc agccaaaagc gttgccggat
    68101 tatacaggca aaaaagcaaa agaagatatc cagtctgttt tgaaagaata tgaacgtcaa
    68161 ggaatcctat ttgtctccgc agctattggc caagacaaag aagaaatcaa gtcgatctac
    68221 ggtgaaagtc gttttgtaga tattactgac ttgaatgaat ttcccaagca aatgattcaa
    68281 ctgattgccc gctatctatg ataaaataaa gctcaacgaa cgcacttagc agattcgttg
    68341 agcttttcgt ttctagttac cggtaacaag gatacttcta aaatagaatc ggttataatc
    68401 aagagggacc gttaaaggag tgaaacgata tgatacaaga acaaaaatgg tggaaagaag
    68461 cagtaggcta tcaaatctat cctgctagtt tcaaagatag caatggcgat ggtcaaggag
    68521 acatcaatgg gatccgtgaa aaacttaatt atttgaaaga tcttggtatc ggctttatct
    68581 ggatcaatcc gatctatcaa tctccatttg tcgataatgg ttatgacgta agtgactatc
    68641 aagatataga agagagattc ggaacgatgg aagattttga tttattgcta aaagaagcac
    68701 atgaattagg gatcaaagtg attatggatc ttgtcatcaa ccatagttcg gatcagcatc
    68761 aatggtttga agaatcgaaa aaatctaaag aaagcccgta tcgcgattat tacatttggg
    68821 tcgacggggt agatggcaaa gaaccgaata attggacatc gattttcggc ggttctgctt
    68881 gggagtattc tcatgaaact ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag
    68941 acttaaattg ggaaagcgag aaattgaaag aagaactatt caatatgatc cgatggtggt
    69001 tggataaagg gatagatgga tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat
    69061 attcagtaaa agccacagag aaccctttct caccctttca aaatgtctca ggaatcgaag
    69121 aacatttgac cgatttgaaa catgtttttg aagagtacga tatcatgact gtcggagaag
    69181 caagtggtgt cactgcagaa gaaggaccac aatgggtagg gaaagacggt tatttcgata
    69241 tgatattcga atttgatcat atccatatct ggcagcaaga aaaagaaggg caattagatg
    69301 tccttaaatt gaaacttgca ttaagcgctt ggcaaacttc attggatggt atcggatgga
    69361 acgctttata tatggaaaac catgatgttc ctcgtgccgt ttcagtgttt ggagatactc
    69421 gtcccgattt ttgggctatg tctgcaaaag caatcgctat gatgtatttc tttttgcaag
    69481 gtacgccatt tatttatcaa ggacaggaaa tcggaatgac gaatatgccc tttgaatcga
    69541 tcgatcaagt tgatgcagta gattcgaaaa gactttataa aaggctgctt gcggaaggta
    69601 agacaagaga agaagcattg gatatcattc gtgaaacaac gagagacaac agcaggacac
    69661 ccatgcaatg gacttcggaa caatatgctg gtttttctac acatgaacct tggctgatca
    69721 ctaatccaaa taccaaaacg atcaatgtag aacaacaaga atatgaacca gagtcggtcc
    69781 tgcaattcta taagaacatg atccgcatta gacaaaccaa taaaggtctg atatacggtt
    69841 cttataaaga atatcttcat gaacatccac aactttatgt ctatgaaaga tatttagaag
    69901 atgaacaata tcttattatg gtaaacttaa cagaatcact tgctgactat gaacttccaa
    69961 aagaagcaga tcagtcctgg acattactct tgtcaaattc ctctagcgga gaatttgaag
    70021 ctaaaggcat actagcacct tatgaagctc ggttatataa aacaaacaag taaaacttcc
    70081 tcgttaaagt tcattagttg gactttgcga ggaagttttt cagattgctc tgtcaattac
    70141 tcatttcaat aattcgctct aggaccaccg atatatttag gacgagagcg aagggctgtc
    70201 acgtgagctt gtccaagggt tttcaaacga ggtctgacag gtacttggac atgtttgata
    70261 tgcataccga ttgatgtgtc accgatatcg ataccggctt gtccaactac gtgttccact
    70321 tcgacaggat cattgaactg gtcaaaagca gcaacagaac aagcacctcc agcatgtagt
    70381 gcaggaacaa cagagacaat ttcgaaattt tttgcttctg ccactgttcg ttctactact
    70441 aatgctcggt tcaaatgttc acaaccttgg acagctagcg caatttcttt aggatccaac
    70501 aactctttta acgtacggat gatccattga ccaacttccg cactggaatt ttttccaata
    70561 tgtcctccaa cgacttcgct tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag
    70621 tgtgcctcag ccaatatgtc attaactatt tcagtcaatt ggtcttttaa ttctttttct
    70681 gacaacttca ttttctaaaa ccttcttttt caattaatat tgcttccgtg cttgaatacg
    70741 ttgaagactt gcagttatag gaaaggcaag tcccattcca atcagatttt ggaagatgtt
    70801 tcctggtata gaagccactc cagccggcca atcatataaa atccaaccgg cgataccata
    70861 gccgatgatc ataactaagc ttcctaataa aagccctaat ccccacatac gagtagaaga
    70921 tttgctgaaa gaaccagcaa catatccttg taacccgtgg atcactaatg aaaaaactgc
    70981 ccactgcgga tatcctgaaa tcaaatcgat caatagacca ctggaagcac caacaactaa
    71041 tccacccgtt ggaccataaa gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac
    71101 aaatccattc gtagccggta cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc
    71161 aatcagcata gcatagatcg ttaattgctt agttttcatt tctcattcct cctttttgaa
    71221 taccgccaga ccagacactt ccggtatgat cgttcatcag cacgccatta gaaatacaat
    71281 gggcaacaaa ttttttactt tctgctactg ctttggtaaa atctgcccct aacacaagat
    71341 tcgctgttat tgcagaagaa aaggcacaac cggccccgtt tactgttgta gtgtcgattt
    71401 ttttatgaga aaatacctca taatgatcaa ttgaatagag aagatccaca gcttcatctc
    71461 cagccatcct tgtgccgcct ttgatcacaa tgttttttgt tcctgaagca gtgagttctt
    71521 tagcgagatg gatcatttct gttaacgaag agggaagagc ttgaccagaa agtaaagcag
    71581 cttctgccaa attaggcgta atcatcgtaa caagtggaaa aagttcttcg atgagctgcg
    71641 aaatatacaa ttgattgatc acagcttcag tttctttgaa tgccaaaaca gggtctaaaa
    71701 tgattgggat attttgtttt gtttttatga agtctcgaac aaccatgatt gcttcacttg
    71761 aattcaataa cccaattttg ataccatcta atgaagtcat tttatcaatc gaaactaact
    71821 gtcgtttaag caattccaaa gatatagatt ccagcataaa tcgctcatta atatctagtg
    71881 cccctataga agtcaaggcg cttagtccga atagaccaaa attctcaaaa gtttttaaat
    71941 ctgtttggat tcctccacca gcaaatggat cagaaccgcc aattgtcaag attcttttca
    72001 taacattttc ctccatgaat aagagtataa cgaaattttc aaaaaagaaa aacagccaat
    72061 tggcatattt ttatctcatc caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga
    72121 tctttctttc cttaaatagt taggactttt gtgattttaa ttctttctcg atttgaagcc
    72181 gttgcacttt cattgtagct gttcgcggga ttgcatcaaa atcacggatg atagggacgt
    72241 tcaagcgagg aagttcatgg acttgctccc accatcgatt ccaatccatt tctttatcag
    72301 gagctaaagc aataatcggt tgtggagaat gatttttatc ccttacaatc actacttctg
    72361 ccaaaaaatc taacgaatct aataaaaagt cttcaatagc taatgtacta ttgatcgttt
    72421 cgatcaaatc cacttgccga tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt
    72481 aatctccgct atcccaccaa tcgccataaa cattttcctg gaaccgtgca tcttccttgt
    72541 aataagtcaa agcccgtcct ttagacagta attgtatatg accatctgtg ttttcaggta
    72601 ggacattccc aactgaatct gtaatccgag cgcttgtcat gtcttccagt ccaacgccca
    72661 tatctcttgc atcacttgtt ttcaaactct ctagtgtatg agccttaaga atcattggtc
    72721 cgcattcact ttggccatat acttgaagaa ataccgcatc ctgtgccatc gacgttcgca
    72781 aaaatgtctt cattgtctgg ttgtttatag catcaaaagt ggagtgatag tatcggatac
    72841 ccgaaaaagc ttctggatgc tccttagcag taaaacgcca ctgaacaaag ttattcggat
    72901 gggtctctag tgcaattggc cggtgagctt ccaacatatg aacaactttt gaactttgtg
    72961 cgtttgctaa aggcatcata ggaaatccca tggacatcaa agaagagata ccaatattga
    73021 atctggaatg cactggagag atgtgaaaag cgattaattt cttttccgaa atttttgtga
    73081 aaactgtttt ttgccatttc gtccgccatc ccatagaatg agcagaatga caaataagtt
    73141 tagggatacc agttgtacca gaagtatggg tcatataaga aatttcattt ttgtctaaaa
    73201 atgattgaga aacagacaaa gcaggttgtt ccattaaata aagaatagg
//
LOCUS       DACEKH010000002        68419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEKH010000002 DACEKH010000000
VERSION     DACEKH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.002"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(7..963)
                     /locus_tag="IW662_000072"
     CDS             complement(7..963)
                     /locus_tag="IW662_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0864927.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            1137..1697
                     /locus_tag="IW662_000073"
     CDS             1137..1697
                     /locus_tag="IW662_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0864928.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            1690..2514
                     /locus_tag="IW662_000074"
     CDS             1690..2514
                     /locus_tag="IW662_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ0864929.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            2524..3141
                     /locus_tag="IW662_000075"
     CDS             2524..3141
                     /locus_tag="IW662_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0864930.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            3159..3842
                     /locus_tag="IW662_000076"
     CDS             3159..3842
                     /locus_tag="IW662_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0864931.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            4525..5808
                     /locus_tag="IW662_000077"
     CDS             4525..5808
                     /locus_tag="IW662_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0864932.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            5824..6711
                     /locus_tag="IW662_000078"
     CDS             5824..6711
                     /locus_tag="IW662_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0864933.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            6728..7564
                     /locus_tag="IW662_000079"
     CDS             6728..7564
                     /locus_tag="IW662_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0864934.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            7567..8190
                     /locus_tag="IW662_000080"
     CDS             7567..8190
                     /locus_tag="IW662_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0864935.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            8187..9479
                     /locus_tag="IW662_000081"
     CDS             8187..9479
                     /locus_tag="IW662_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0864936.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      9838..9935
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10051..10632
                     /locus_tag="IW662_000082"
     CDS             10051..10632
                     /locus_tag="IW662_000082"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ0864937.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            10635..11942
                     /locus_tag="IW662_000083"
     CDS             10635..11942
                     /locus_tag="IW662_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ0864938.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            12358..12846
                     /gene="purE"
                     /locus_tag="IW662_000084"
     CDS             12358..12846
                     /gene="purE"
                     /locus_tag="IW662_000084"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ0864939.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            12830..13963
                     /gene="purK"
                     /locus_tag="IW662_000085"
     CDS             12830..13963
                     /gene="purK"
                     /locus_tag="IW662_000085"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ0864940.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            13976..15271
                     /gene="purB"
                     /locus_tag="IW662_000086"
     CDS             13976..15271
                     /gene="purB"
                     /locus_tag="IW662_000086"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ0864941.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(15451..15828)
                     /locus_tag="IW662_000087"
     CDS             complement(15451..15828)
                     /locus_tag="IW662_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0864942.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            16084..16812
                     /locus_tag="IW662_000088"
     CDS             16084..16812
                     /locus_tag="IW662_000088"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ0864943.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            16812..17066
                     /gene="purS"
                     /locus_tag="IW662_000089"
     CDS             16812..17066
                     /gene="purS"
                     /locus_tag="IW662_000089"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ0864944.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            17068..17739
                     /gene="purQ"
                     /locus_tag="IW662_000090"
     CDS             17068..17739
                     /gene="purQ"
                     /locus_tag="IW662_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ0864945.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            17740..19962
                     /gene="purL"
                     /locus_tag="IW662_000091"
     CDS             17740..19962
                     /gene="purL"
                     /locus_tag="IW662_000091"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ0864946.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            19947..21386
                     /locus_tag="IW662_000092"
     CDS             19947..21386
                     /locus_tag="IW662_000092"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ0864947.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            21418..22461
                     /locus_tag="IW662_000093"
     CDS             21418..22461
                     /locus_tag="IW662_000093"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ0864948.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            22458..23036
                     /gene="purN"
                     /locus_tag="IW662_000094"
     CDS             22458..23036
                     /gene="purN"
                     /locus_tag="IW662_000094"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ0864949.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            23033..24568
                     /gene="purH"
                     /locus_tag="IW662_000095"
     CDS             23033..24568
                     /gene="purH"
                     /locus_tag="IW662_000095"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ0864950.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            24584..25858
                     /gene="purD"
                     /locus_tag="IW662_000096"
     CDS             24584..25858
                     /gene="purD"
                     /locus_tag="IW662_000096"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ0864951.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            25980..26894
                     /locus_tag="IW662_000097"
     CDS             25980..26894
                     /locus_tag="IW662_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ0864952.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(27027..28592)
                     /locus_tag="IW662_000098"
     CDS             complement(27027..28592)
                     /locus_tag="IW662_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0864953.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(28585..30036)
                     /locus_tag="IW662_000099"
     CDS             complement(28585..30036)
                     /locus_tag="IW662_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0864954.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(30050..31066)
                     /locus_tag="IW662_000100"
     CDS             complement(30050..31066)
                     /locus_tag="IW662_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0864955.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            31286..32374
                     /locus_tag="IW662_000101"
     CDS             31286..32374
                     /locus_tag="IW662_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0864956.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            32428..33954
                     /locus_tag="IW662_000102"
     CDS             32428..33954
                     /locus_tag="IW662_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ0864957.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            33956..35161
                     /locus_tag="IW662_000103"
     CDS             33956..35161
                     /locus_tag="IW662_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0864958.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            35418..36395
                     /gene="guaC"
                     /locus_tag="IW662_000104"
     CDS             35418..36395
                     /gene="guaC"
                     /locus_tag="IW662_000104"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ0864959.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            36659..38083
                     /locus_tag="IW662_000105"
     CDS             36659..38083
                     /locus_tag="IW662_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0864960.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            38544..39653
                     /gene="pdhA"
                     /locus_tag="IW662_000106"
     CDS             38544..39653
                     /gene="pdhA"
                     /locus_tag="IW662_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ0864961.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            39656..40633
                     /locus_tag="IW662_000107"
     CDS             39656..40633
                     /locus_tag="IW662_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ0864962.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            40658..41953
                     /locus_tag="IW662_000108"
     CDS             40658..41953
                     /locus_tag="IW662_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAQ0864963.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            41961..43367
                     /gene="lpdA"
                     /locus_tag="IW662_000109"
     CDS             41961..43367
                     /gene="lpdA"
                     /locus_tag="IW662_000109"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ0864964.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            43529..43804
                     /locus_tag="IW662_000110"
     CDS             43529..43804
                     /locus_tag="IW662_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ0864965.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            43810..44577
                     /locus_tag="IW662_000111"
     CDS             43810..44577
                     /locus_tag="IW662_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ0864966.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            44745..46574
                     /gene="typA"
                     /locus_tag="IW662_000112"
     CDS             44745..46574
                     /gene="typA"
                     /locus_tag="IW662_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ0864967.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            46835..48607
                     /gene="recQ"
                     /locus_tag="IW662_000113"
     CDS             46835..48607
                     /gene="recQ"
                     /locus_tag="IW662_000113"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ0864968.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            48721..49023
                     /locus_tag="IW662_000114"
     CDS             48721..49023
                     /locus_tag="IW662_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ0864969.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            49026..50189
                     /locus_tag="IW662_000115"
     CDS             49026..50189
                     /locus_tag="IW662_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0864970.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            50215..53643
                     /locus_tag="IW662_000116"
     CDS             50215..53643
                     /locus_tag="IW662_000116"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ0864971.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            53664..54788
                     /locus_tag="IW662_000117"
     CDS             53664..54788
                     /locus_tag="IW662_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0864972.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            54778..55215
                     /locus_tag="IW662_000118"
     CDS             54778..55215
                     /locus_tag="IW662_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0864973.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            55199..55537
                     /locus_tag="IW662_000119"
     CDS             55199..55537
                     /locus_tag="IW662_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ0864974.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            55656..56216
                     /gene="rsmD"
                     /locus_tag="IW662_000120"
     CDS             55656..56216
                     /gene="rsmD"
                     /locus_tag="IW662_000120"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ0864975.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            56213..56704
                     /gene="coaD"
                     /locus_tag="IW662_000121"
     CDS             56213..56704
                     /gene="coaD"
                     /locus_tag="IW662_000121"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ0864976.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            56694..57737
                     /locus_tag="IW662_000122"
     CDS             56694..57737
                     /locus_tag="IW662_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0864977.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            57896..58624
                     /locus_tag="IW662_000123"
     CDS             57896..58624
                     /locus_tag="IW662_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ0864978.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            58740..58985
                     /locus_tag="IW662_000124"
     CDS             58740..58985
                     /locus_tag="IW662_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0864979.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            58982..59200
                     /locus_tag="IW662_000125"
     CDS             58982..59200
                     /locus_tag="IW662_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ0864980.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            59336..59875
                     /locus_tag="IW662_000126"
     CDS             59336..59875
                     /locus_tag="IW662_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ0864981.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            59868..62141
                     /locus_tag="IW662_000127"
     CDS             59868..62141
                     /locus_tag="IW662_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ0864982.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            62179..63225
                     /gene="holA"
                     /locus_tag="IW662_000128"
     CDS             62179..63225
                     /gene="holA"
                     /locus_tag="IW662_000128"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ0864983.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(63314..63568)
                     /locus_tag="IW662_000129"
     CDS             complement(63314..63568)
                     /locus_tag="IW662_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ0864984.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(63968..64237)
                     /locus_tag="IW662_000130"
     CDS             complement(63968..64237)
                     /locus_tag="IW662_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0864985.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(64707..65162)
                     /locus_tag="IW662_000131"
     CDS             complement(64707..65162)
                     /locus_tag="IW662_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ0864986.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(65300..67228)
                     /locus_tag="IW662_000132"
     CDS             complement(65300..67228)
                     /locus_tag="IW662_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0864987.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(67425..68258)
                     /locus_tag="IW662_000133"
     CDS             complement(67425..68258)
                     /locus_tag="IW662_000133"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0864988.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT        22257 a        11763 c        14939 g        19460 t
ORIGIN      
        1 ttaaaattac atatttttcc atagaacttg catttgatcc aagatttcga tgacatcttt
       61 tgtcaagaag agagagcgat tgggcaagtc accttcaatc atgtgtacca tattctttat
      121 ttcgtaattc attgcttcag atgtattccc gctttcgatg aattcctttg taccatctgt
      181 aaataagatc tccgcacgat cagcacgcgg gtaatcagca atcgtcacat aaccgttctc
      241 aaaggcaacg ataccgactt ttggcatttt tgcctgaaaa gtcaagctga cagcagcaag
      301 ttcattttct ttgttacgca agatagtgac ggattgttcg tccacgcctg tttcaaaagg
      361 aaccattgta gaggcaacta cttcaggttg agagctcaaa aagaaacgag cgaaagatac
      421 agcatacgta ccgatatcca acagagctcc tccagctaat tctggattga agaaccggtt
      481 tttagggtct ggttctttgt aactgccgaa aggtgcttgg atcattttta aagcccccag
      541 ttttcctgta tccattattg agcgtaactg ctgataaagt ggcatgttga agattgtcat
      601 cgcttctgct aagatcaaat tcttttcttc tgctaagcgc atagcgtcag ccagttcttt
      661 tttattcatc gtgatggctt tttcacatag aacgtgtttg cctgcttcca atgctgccaa
      721 gatatggtcg atatgctgtc tatttggtac agcaatgtac acgatgtcga tttcagcatc
      781 gtctaacatt tcttgatagc ttccgtaagc ttttggaata ttgtaacggt gagcgaagtt
      841 ttccgctttt tctgaagttc tggaagcaac tgctgttagt tcgctcgtct cttgattgaa
      901 gctttcggca aactcatgtg cgatggtacc aagaccaacg attccccatt gatatttttt
      961 catctgaaaa cgctccttca tagttggatt gagtttcttg tctgtctatt attataacaa
     1021 aaatcacgaa gaataaagag gagaggactt gaaacattaa aaaaaatgaa agcagcgatt
     1081 taagaggtgc tttttgatca aaacttaagt agaatagtta aaaggagggg ataaacgtgc
     1141 atcaagtata tattgatctt attatgagtg tattattaga acagtttgaa actgaaaaaa
     1201 attttttaga tgaatacttg ctgatcagtc cagaacaatg gcaaaaatgg aaagatggcg
     1261 agaaaaatct gccatctgaa gcgatgcaga aagtaaaaaa tcttttcact gattatgaat
     1321 ggatgctaac gcaaaagatt ttgcgtcaaa cgattatttt tcccgaaaaa cgaaacgttg
     1381 ccgttgcaga gtacaagcaa atcaaaacac gcatcgcgca aaaatggctt agcagcaaag
     1441 cagcaaaagt cgagctgatc cctttgaaaa ataatcagca ggctggtaga taccttgact
     1501 tgaaagtaag tattcaatat gatgaatggg gctatgatga tattttgaat tttcgtctgc
     1561 cagcagatgt tcaaaaacag atcgaaggag aaaaaatcga acttctcgat tgggtgaatg
     1621 aaaatctgga agaaacttac gttcatgagg cgaatgggga acgtgaagaa caacgaaagg
     1681 atttttcaag tgaataaatt tttaggattt ggagcagttg tgattatgat cgcagcaatg
     1741 ggctatgtta tttttagcga tgatgattcg catgttcaag ccggcaatca agcagtcgaa
     1801 cgaacaagca ccaaaacaac tgctagtaaa gaaaagaaca gtcaaacaac cagcagcaag
     1861 gtaaaggaca aagaaacaaa agatcgtacg tccaaacaat tgccagatgt taaaacagac
     1921 gattggaagc tagttttggt tggtccagat cacaaattag aaaaagaaat tgatgagtct
     1981 actcaattgg catcattaag taacggatac ttgatcgaca aacgcattgc tccagaatat
     2041 gaagcatttg ccaaggctgc tgaagcagct ggatttccat tagtgatggt ctcagcttac
     2101 cggtctgtat catcccaaca acaagttttc gaacaaaatg tccaagatgt gatgagtcgt
     2161 caaggtgtat cagaagagga ggcgacaaag attactaaag aaacgattac ggagccagga
     2221 tacagcgaac accacacagg tctggcagtc gatgtcgtcg atcaaaactg gtacaacagt
     2281 tatccttcta ctgtattgga tgcaagttac ggagatcagc ctggagctaa atggatcgca
     2341 gataatgctg cgcagtatgg atttatcgtt cgttatccaa aaggacacga agatataact
     2401 aagatcacat atgaaccttg gcacttacgt tatgtcggta aagaaaacgc tgagtatatc
     2461 accaaacacc aattgacgct agaagaatat ctgaatgaat taaatgaaaa gtaggaattg
     2521 atcatggcga aaaaaaagtg gaaaaagcaa aagcgcaaga atatccgttg gccagcagtg
     2581 gttgccggtg tagcagtgat actcttagcc tttgtttttt ccttgaagaa tctgtcttct
     2641 ccttttactg ataatcagca agaaaatgaa gcgcaatccc atcagcaatt tattgaacgg
     2701 cttgttccgc atgcccaaga actacaagat ggttatggag tgcttcctag tatcatttta
     2761 ggacaagcta tattagaatc aaattgggga aaaagtacct tggctagcaa atacaataat
     2821 cttttcggga tcaaggctta tggggatcag aagaaagtaa gcctggaaac aaaggaattt
     2881 gttaacgaag aatggataac tatccaagga gacttcaaag tgtatgattc atgggaacag
     2941 tccatggatg accatacaca actgttcgtc caaggagtag attggaatcc tgcgctgtat
     3001 gaaaaagtga ttacggccac caactatcag gaagccgcac aagcattgca agatgcaggt
     3061 tatgcgacag accccggtta tgcgcaaaaa atcatccagg tcatcgagac gtatcaattg
     3121 aatcagtatg accggagata gcaaaggaga agtggctgat ggcagaacaa tttacgccag
     3181 aaatcatgac ggaattgaga aaagatatcg taagagtacc ggaaatcatt ttagaagcaa
     3241 gcgggatcaa gatatttgga aaattgatcc gttcgatcat ctttactacg gatattgcga
     3301 tcatacgtaa tacaaatgcg aatgcagtaa ttgctgtgta tccttttaca cctcatccag
     3361 cgattactaa aagcattatt gaggcagctg acattccagt tttctccggt gtaggtggtg
     3421 gtttgacaca aggtgctcgt tcttcctaca tgagcatgtt cgcagaagcg caaggttcgt
     3481 taggggtcgt tttgaacggt cctacgcctt tagagacagt aaaagaagtt tgtcgagtag
     3541 ttgatattcc agttatcagt acggtaacgt cgaaatatac agaaatagat gaaaagttag
     3601 cagctggtat ttcgattatc aatatcagtg caggtaaaga aactgcagaa gcagtacgcc
     3661 attttcgtaa aaaatatcct gaacttccta tcattgcgac aggtggtcca acggaagaaa
     3721 gtattgctga gaccattcaa gcaggagcaa atgccatcac atatacgcca ccaagtagcg
     3781 gagagctttt tagtcgtaaa atggaaaaat atcgggaaac tgagaaaaag aatcatgaat
     3841 aacaggtgag aatcaaaaga cgaggaagag cagatagtgg ctcttcctcg tcttttgtac
     3901 tccagtacta tttgttcaga attcaaaaaa aagagaaatt tttttgtttt tttacctgta
     3961 atgtacataa gaagagttaa aaccttttgt tattattagc attctaacaa aaaaatgtct
     4021 tgatttattg aagcgctttc tatagaatga aaaacgaata aaaactaaaa aaggcacccg
     4081 acaatttagt agcagctaaa ttgtcggggc ttgaaaagtc agcgggaact gaccaatcaa
     4141 gttgccatag tcaatgcttg tgcatcgacg tgtatattgt acctaatttc atacagaatt
     4201 actagttatt tttctgtttt ttagggaaga gcgcacacga agaagcatgc ttatttagct
     4261 atgtcaagga tattgggcag ttgtctgata tccttttttc tttgcgtaat tatcctccca
     4321 tcattgaagc aagaagtgtt tttagcgatg aaagaagaat cagcactatc taatttttct
     4381 tgaaagtatt gagttgttca tcctaggcag aacgataagg caagtcttgg tttttattcc
     4441 ccttgaaaaa gatcgtttaa taaatatgtt ctgtataaaa atacagtata ttgaaaaata
     4501 acaatggaag tagaggtgca gagaatgaag aaaagaatga gaatcatctt agctttaagt
     4561 gttatgttat tcttcttgac ggcttgtggg caagaagatg acaaaaaaat cacgctgaac
     4621 atggcttggt ggggttcgca agtccgacac gatgcaacag taaaagtaat cgagctttat
     4681 gaagaaaaaa atccccatgt gacgatcgat tatgaatttt atgattatga aggatattac
     4741 acaaaattag gtacactagc agcatcagat gatatctggg acattttcca actgaacaat
     4801 caatttcctc aatacatcaa tcagattgaa ccattagatg aatatgtgga gcaagggata
     4861 attgatattt ctaatgcaga tgaatcctat ttagcaacga cgacttacga cgggcaatta
     4921 gttggactgt ctaacggggt gaattcgttt gcgattgctt acaataaaaa tatatttgaa
     4981 caattaaata tccctgaacc agcttcaaat tggacatggg atgaatttga acaaatcagc
     5041 tatcaaatca ctgaagaatt aggaatctat gctatttcaa gattcgaaga ttttatagct
     5101 ggctgtatta ttcaaatacc acaagttgag aaaggattga atttttataa tcgggaagat
     5161 ctatctaaca gcttgggatt tgaaaatcca gactatctaa cagatttttt ccagctgcgt
     5221 aaaaatctgg tagacaaagg ggcccatcca gaaccaggtg gtggagctgc aagtacgacg
     5281 gatctgtctt ttcaggcggt tagggataga gaagcggcaa tggtgtttct atcaagtaac
     5341 cagttgacag aaatattgtc aggggctcct gaagatatgg agattcagtt gattaatcca
     5401 cctcgcagaa aagcagatgg agaatctggt atccctttac gttcttcgca aatgctgagt
     5461 atggcaaaaa gctcagccca taaagaagaa gctgctaagt ttattgattt ctttcaaaac
     5521 agtgaagaag ccaatgaaat tttaaaagga gaacgtggtg taccaatcat gtcgaatatt
     5581 cgagaacagc tcgctcgtgc agatgatgca actttgcaag cgacatttga ttatttagaa
     5641 atgatttccg atatggacaa cggcgaagtg aatgtctttg atagtgcttt gaatccggaa
     5701 attcaagacc aatacaatct tttagttgaa aaagtcattt atgaagaagt aacagcagaa
     5761 gaagcagcag agagtcttta tgagtttgcg tcaaacattc taggatagca gcaggaggaa
     5821 ggcatgaaaa aagaaaaact aaaacggttc ttggatcaag acaatgtggt cggttacgta
     5881 tttgcttctc cattcatcct aggatttcta ttgataacag tgataccgat gatcttgtca
     5941 ctttattatt cgttcaccga ttatcgtttg ggaaatccag tctcatggat cggcttggat
     6001 aattatataa agatgctgag tgatgcccgt tttttgaact ccatccgagt cacttttctc
     6061 tatgtatttg catctgtacc tgtgaaactg atctttggtc tattgattgc ttatctttta
     6121 acgaggaata tcaaaggaac gactttcttt cgttctttgt attatgtgcc tagtttgatc
     6181 ggtggcagta tttctatcgc tctagtttgg cgggagttgt tttctaaaaa cgggttagtg
     6241 aatattttcc tggaacgatt aggcgcacct gcgctttcct ggtttagcga tcaaaaactg
     6301 gtcatgatcc ctcttgtttt gatgtctgct tggcaattcg gttcggcaat gatcatcttt
     6361 gctgcaggta tcaaagagat cccaggctcg tattatgaag cggcagagat cgatggagcg
     6421 aacaaatggc agacgtttta caagatcacg ttacctcttc tttcgccgat cattttgtat
     6481 aacttggtta tgcagacaat cgcggcgttt atgactttta cgcaggcatt tatcattacc
     6541 caaggtggtc cgaatgatgc cacgaacttt tatgctcttt atgtgtataa ccaagctttt
     6601 tcttttaatt ctatggggta tgctagtgct atttcgtgga tcatgctgtt gattatggga
     6661 gctgtcacct ttctgatttt caaatcatca aaaaaatggg tcgttgtgtg aatgagagga
     6721 ggaagaaatg aataatacag taacgaaatg gtttcaatac ggttttattt tgctattagg
     6781 tctagtcatg atttatccgg tcttatggat gatcgcaggt tcttttaaaa cggatcaaga
     6841 gattatgagt ggttcgctga atttgattcc agagactttt caatgggaaa attacgcaaa
     6901 aggatgggcg gggtttgctg gaatttcatt ttttactttc tttaggaatt cgtttgtcat
     6961 ctctgcagtt tcgacgatag ggacagttct gagctcgact tgtattgctt acgcattatc
     7021 gagaatcgat ttcaaaggaa aaagattctg gttcgtcgtc atgttagtaa cgatgatgat
     7081 tcctgcacaa gtgattttga ttccgcagtt tattatttac aatcggctga atttggttgg
     7141 gacgtatgtg ccgctgattt taccacattt ttttggacaa gcatttttca tttaccagat
     7201 catgcaattc atggtcagca tccccaaaga gttagatgaa tcagcgatta ttgacggatg
     7261 cagcaagtat agtgtcttta ccaaaattat ttttcctttg ctgaaacctt cgatcatcac
     7321 gacgatcatc atccagtttt attggaaatg ggacgacttc atgggaccat tgatttattt
     7381 aaacaagccg agatcttaca cggtatcgat tgccatcaaa ttatttgcag acgctggatc
     7441 tagtacgact tacgcgagta tgtttgcgat gtctacgttg tcgctgctac cggtttttct
     7501 gattttctta ttcttcaata aatatcttgt ccaggggatc agtacaagcg gtctgaaggg
     7561 ctgaatatga aaaagccaat agaagagatg tttctttaca agctcaacta ctatgtctgt
     7621 cggatttttt taaccagtct tatcagtaat gctcttttcg ctttagttct aggatgtttg
     7681 ttcactttag gattagtgct gtctgctcgt gttgtctatc tgatttattt tttgatgatg
     7741 tatccttctt attatctatc tgtccattac ttggcagaaa aaaaggtatg ttggaggaaa
     7801 ggaaaaatca aacggatgtg tttcatcatt acgttgtttc agctgtttgt cattattttt
     7861 gcagttgatt ggtatttatt agtgggaaag attggagctt ggtatctgat tccttttcta
     7921 ttgtgtctgt tgctttttgt atcccatagt ttttttagta cgctaattga tatctgcaaa
     7981 gaagataaag cagatagtct tgtttatttg atcaaaagga atatctggct gttgtcaaaa
     8041 aacgtcaagg ggtttctgtg gtatttatgc cttttgtatg gactgttctg gtgtatacag
     8101 catgattttt tactcactgc attactgtat ccagggctgc catttgtcac agtattttgc
     8161 ttaaaagaaa aggagaatga ctttaaatga agaaaaaata tgtacaagtc ggacttggtg
     8221 ggagagcgcg ttttttttat gaggcaattg cctctacgta tcatcaaact tgtgaactag
     8281 tcggtttttg tgatgtatct caaacaagga tggattatgc aaatcggata cttgtgaaca
     8341 agtatggaca gaaaaaagta aagacatgta tgccatcaca atttgagcaa ctactggatg
     8401 acgtacggcc ggctgaagtc attattacgt cagtagatcg aacgcatcat gattatatca
     8461 ttcgtgcaat gaaaaaagga tatgatgtta tttgcgaaaa acctctgact atcgatgagg
     8521 aaaaagctca agaaataata acggtccaaa aacagacggg aagaagcctg cgagtcactt
     8581 ttaattatcg ttatgcaccg tatcacacga agataaagga actgatagaa agtgatgtga
     8641 ttggtgatgt gttttctatc cacttcgaat ggctgttaaa tacgcaacac ggggcagatt
     8701 atttcagaag atggcatcga aataagcaca atagcggcgg gcttcttgtt cataaatcta
     8761 cccatcattt tgatctagtg aatttttggt tgaacagccg tccccaaaag gtatttgcat
     8821 tcggtggatt aaacttttat gggatgcata atgcggagaa acgcggagta gaaatatttt
     8881 acagtcgatg ctatggaagc gaactagcta aagctgatcc atttgctata gacctgtcag
     8941 ccaatgagga attgcgagaa atgtatttag aagcagaaaa agacagtgga tatattcgtg
     9001 accgcagtgt attttccgat gatatctcta ttgaagatac actaggcgta acggtcaaat
     9061 atgaaactgg tgcgattttg acgtattcgc taaacgcgta tatgccgtgg gaaggattca
     9121 atgtttcttt caatggcagc aaaggacgga tcgaaatgaa agtgatcgaa aagtcttaca
     9181 tcaatgcagg tggagataaa aaggatgaag gagcagccgt tcttcagtcc atccatgtct
     9241 atccgatgtt cggtgagcca tatgaggtaa tcgtcgaaca agaagcgggc ggacatggcg
     9301 gaggagatcc gcgtttatta gatgatctat ttggtgaaga aaaagaagaa gatccgtgga
     9361 atcgggcagc aactcacgta gatggtattt tgtcgatttt gacaggaatc gcagcaaatc
     9421 attctattgc ttcaggaaaa gcagtagtga ttgacgagct tgtcagctta aaagaataag
     9481 aatagattta cggaaattac ttcccgtagt ttataaaatc ttgccttatt gccgataggc
     9541 tgtcttttga acactattta ttcggaaaaa ggggctatga catgcttttt gtcatgcctt
     9601 ctttttatgt gggctgcttt tttagatagg agaaaataaa aataactatt taaacccgaa
     9661 caaaaaggaa taatgcatta aaaatcatta gattttacaa aaaaataagt aaaccacgtt
     9721 gtcagtttca gaaagtagtg ttagactgtt ttcaatattt gaaacaacat tttttattca
     9781 aatatggcaa actgccttca cgtgcaaaac agttgcaaga aaatgaattt acactattat
     9841 tagttttaca tacctactta tatatcgtca taatatggat gacagtttct agccagtacc
     9901 gtaaatgctg gactataagt aaaagattgg ctatttcacc cttcttttct tatgataaag
     9961 tggagggtgt ttttctttaa cagaaacata gtaggttttt gcacaaaagc ggttatttcg
    10021 gtattaataa atgataaagg agcaaataaa gtgaaagaat tagttgaacg tattcaaaaa
    10081 gacggacgta tattaggcga gggcgtatta aaagtcgaca gcttcgtcac tcaccaagta
    10141 gaccctgtat tgatggaaca aatgggaaaa cggtttgcag aagtatttaa agaacaagga
    10201 atcacgaaag ttgtgaccat tgaagcttct gggatcgcac cagctttatt tgctgcaaga
    10261 gaattagatg ttccaatgat cttcgctaga aaagcaaaaa gtttgacgat ggatgaagaa
    10321 ttattgacag cttctgttta ttcttttacg aaacaagtga caagtacgat ttctatttct
    10381 cgtaaatttt tatcttcaga agacaaagta ttgattatcg acgatttctt ggcaaacgga
    10441 caagcggcaa aaggattgat cgaattatgc cagcaagctg gagcgcaagt agaaggaatc
    10501 gggatcgtca tcgaaaagtc tttccaagat ggacgtcagt tacttgaaga aatgggattg
    10561 agagtcgttt ctcttgcgcg tattgcttcc ttgacgaatg gacaagttga gtttcttgag
    10621 gaggacgcat aatcgtggaa aaagaaacac aaaatggaaa agctgcggtt ttagggctgc
    10681 agcatctgct agcgatgtat gcaggagcag tagcggtgcc tcttttgatc ggtacaggac
    10741 taggctttaa tcaagaacag atgacttatt tgatttcaat tgatatcttt atgtgtggat
    10801 tagcgacatt attgcaattg acagttaacc gatttttcgg aattggcttg cctgtcgttt
    10861 taggatgtgc gatccaagca gtagctccac tgatcttgat cggaaccaat gaaggagtag
    10921 gagctatcta cggatctatt atcgcttctg ggatctttgt tgtgttagtt tctggtgtct
    10981 tttctaaaat taaaaaacta ttccctccta tagtaacagg aacggttatc accgttattg
    11041 gtttgacttt gattcctgta gcaatcgaga aaatgggtgg aggtaatgca acagcagctg
    11101 attttggtga caaaacaaat ctgctacttg catttgtgac gattctccta attgtaggcg
    11161 tgcaaatgct ggcaaaaggg ttcattcgtt caatcgcggt attgattggt ttagtcggag
    11221 gaagcatctt agctgctttc atgggaatga ttgatctgag cgcgattgga gatgcgccgt
    11281 tgttccatgt tccacaaccc ttttattttg gtaaaccaac tttcgacgta tggtcgattt
    11341 tactaatgat catcatttcc atggtcagca tggtagaatc aacaggtgta tattttgcac
    11401 ttggtgacat tactgggaaa aatgttggag aagaagaatt gaaaaaggga tatcgtgcag
    11461 aaggacttgc cgttatctta ggcggtattt tcaatacatt cccttatact ggcttttctc
    11521 aaaatgtcgg attggttcaa ctttcgggaa tcaaaacaag aaaacccatc tatttctctg
    11581 cattcttctt gattgtttta ggattattcc ctaaaatcgg tgcgttagca caaatcatcc
    11641 ctgagccggt acttggtgga ggaatgttag tcatgttcgg gatggtggca gtccaaggaa
    11701 tccgtatgct gttggaagtt gatttcagca atgataaaaa tctattgatt gttgctgttt
    11761 ctgttggatt tgggctgggt ttcaatgtta cgccgacatt attcagtcaa atgccgcaaa
    11821 cattgcaaat gttcactgga aatggcatcg taatgagtag cattacggct atcatattga
    11881 atttgatctt taatggattg aaaaaagatg aagaaatatt ggaagaagcc ttcaagaagt
    11941 aatagagcaa aagaaaggtt gtgaaatcag cgttttgacc tgattgttag agaaacaaac
    12001 ggaaaaatag ttcctcgtat tttgccgttt gtttgagcta ataccgaggg tcagctgatt
    12061 gaacaccgtt gaaggaagag gtcgtgagcc tgccgttcat ctccgagtca caaggaacaa
    12121 tttcaaaaaa aagcttctca tgtttgttga atattgttcc ttgtgaccga ggagttggca
    12181 ggagaacacc gtttatccat aaaaaggaat gtgacctaac ttttgtcaca ttcctttttt
    12241 ttatctttaa taaacacgaa caataaaatg tttttctttg ttaacattcg aaatttcttt
    12301 gacatcaaaa aaagtgattg ttaaaatatg aatgtagaaa aactgggagg aaaaacaatg
    12361 acagcaaaaa tctctgtgat tatggggagt acttcagatt gggaaacaat gaaagaagca
    12421 tgtcaaatat tggatgaatt cggtgtgtct tatgaaaaaa aggtcgtttc tgctcatcga
    12481 accccagatt tgatgtttga atatgcacaa aacgctcgaa aaaaaggaat caaaatcatt
    12541 attgcaggag cagggggagc cgcgcatctg ccgggtatgg tcgcatcaaa aacaactttg
    12601 ccggttatcg gtgtaccggt ccaatcacga acattaaatg gtttggattc gcttctttcg
    12661 attgtccaaa tgcctggagg agtacctgta gctactacag caatcggaaa agcgggagca
    12721 attaatgctg gactacttgc gatacagatg ctttccatgt atgatttaga attggaggct
    12781 aaattggctg aaagacgttc aatgttggct aaaactgtta tagaaagtag tgatcaactt
    12841 ggttaaacct ttgttgcctg gttcaacgat cgggatcgtc ggtggcggtc aattaggtcg
    12901 tatgatggcg attagtgcaa aagaaatggg ctttaaagta ggtgtgctgg atcctgtgag
    12961 cgattgtcca gccgcacaag ttgcagattg gcacattgaa agtgtctatg atgacacctt
    13021 tgcgctgaaa gagcttgcca gaagaacaga tgtcattacc tatgaattcg aaaacgtcag
    13081 tatagaggcg ttaaatgcga ttttacccat gtcatttatt cctcaaggga cagacttgct
    13141 tgcgattacg caagaccgcc tgttagaaaa atcttttttg gaaacgaata acatcgtgat
    13201 tgctccttat gcaacgatcg tcagtccgac ggatatccaa gatgcgattg acgggatcgg
    13261 ctatccttgt gttttgaaaa ccactcgagg cggctatgat ggcaaggggc agtatgtttt
    13321 gaaaagtcgg gcggatctag ctcctgccat ggacctttta agagaaggaa cctgtgtttt
    13381 agaagcatgg atcccgtttg aaaaagagat ttcgatcatg gtggcaggaa atggtcaagg
    13441 agatttcacg acttttccgg tcgtggaaaa catccaccac aacaatattc ttcatgaaac
    13501 gattgctcca gccgcgattg atcaggatgt gatcgaggag gctgagcgta ttgcccgtgt
    13561 gattgcagaa gcagtgagcc tttcaggtgt tttaggggta gaaatgtttt taacaaaaac
    13621 aggcgggcta tatgtgaatg aattagctcc tcgtcctcac aattctggac attattctat
    13681 tgaagcttgt tctatgagtc agttcgatgc ccatattcga ggtatttgtg gttggccatt
    13741 aggagaaaaa gtacgtttga taagcaatgc agtaatggtc aatgttttgg gagaacaact
    13801 gctggaaagc tatcgcttga ttgagaataa gccagactgg caattccatt tctatggaaa
    13861 aaaacaagca aaaaaaggtc gcaagatggg acatatcact atcttgacag aagacatcca
    13921 gcaaacgctg gaagaaatca aacaaacgaa tatttgggat tagaggagaa aaaacatgtt
    13981 agaacgttat acacgccctg aaatggggaa tatctggacg gaagagaaca aataccgtgc
    14041 gtggctcgaa gtcgagattt tagcagatga agcttgggca gaacttggtg aaatcccgca
    14101 agaagatgtg aaaaaaattc gtgagcatgc atcatttgat atcgatcgga tcttggaaat
    14161 cgaaaaatta acacgccatg atgtcgttgc gtttacacga gcggtttctg aaacattggg
    14221 agaagaaaga aaatgggtcc attatggtct aacaagtacg gatgtcgtag atactgctta
    14281 tggctatctc atcaagcaag caaatgatat tttacgggaa gacttgaaac gttttacgga
    14341 aatcattggc gaaaaagcaa aagaacacaa atatacagtg atgatgggtc ggacacacgg
    14401 tgtccatgct gaaccaacta cttttggatt gaaacttgcc acatggtatt cagaaatgaa
    14461 acggaacatc gaacgatttg aacatgcggc aaaaggagta gaagcaggaa aaatctcagg
    14521 agcagtggga acgttcgcaa atatcccgcc atttattgaa gagtatgtat gtaaacaatt
    14581 ggggatccgt ccacaagaaa tttctactca agttctccct cgggatctac atgctgaata
    14641 tttctcagcg atggcgctta ttgctacaag tattgaacgt tttgcaacag agatacgcgg
    14701 attgcaaaaa tccgaaacac gtgaagtaga agaatttttt gcgaaaggtc aaaaaggttc
    14761 ttctgctatg ccgcacaaaa ggaatccgat cggttcagaa aatatggctg gtctagccag
    14821 agtggtgcgc ggacacatgg tcactgccta tgaaaatgtc acattatggc atgaaagaga
    14881 tatttctcat tcttcagcag aacgaatcat cttgcctgat accacgattt tattgaatta
    14941 catgttaaat cgttttggca atatcgttaa aaatctaacg gtctttcctg aaaacatgaa
    15001 gcggaatatg tctgcaacat ttggtttgat ttatagccag cgcgttatgc tgaaattgat
    15061 cgataaggga atgacaagag aagaagctta tgatcttgtt cagccaaaaa ctgcgtacgc
    15121 ttgggacaac caagtgcagt tccgcccgct gttagaagaa gatccaacca tcacatcggt
    15181 attatctaaa gaagaaatag acgatgcctt tgattatcat catcatttga aaaacgttga
    15241 cgtgatattc aaacgagtag gtttggaata aagaagagtg agttgcaaaa ttcaattaaa
    15301 atagaatagc aaaaataacc aaaaaagaag cagatccgtt agcggttctg cttcttttag
    15361 tttattatat tttcttatca ataataggaa cctgatttta aaagcggcta cctctttacc
    15421 gtgcctcttt acatacaaaa aatagaggaa ttaagcgtaa atggcttggc tattataact
    15481 aaaatgctgg ttatggagat gttgcagcaa gttttcgaga aaagcgtatt ccatctcttg
    15541 tccattgaat aagatctgga tacgataatg atttttgaaa cgcttctgtt catgaatctg
    15601 actgattgaa agttcgcctt tgtgttctgt ctgataaaga aaagtccttt tacggcattc
    15661 agcacagcgc ttggttgctc ggcggatttt tcggaatgta taaaaactag tatctttatt
    15721 gcataaataa acgtctttga tcgacagcaa atcattatat agacaggaac gttcttcagc
    15781 tgttaattct gataaaaaaa gctgaccttg ttttattgca tcttccatcg gcaagtgcct
    15841 cctcgaaccc attatccaaa tttttagtta attctaatat accatcatta agtataaatg
    15901 taaacgcttg ttgatggtat aagattttcg tttctgataa aacacgaaca ttaaaagaat
    15961 ctaatgcctt aaatgtacat atttttgttg atttataaaa agatgattgc taaaatttaa
    16021 agtgaaaaaa cgaacaaaaa acgatgtatt tgcttttaat gtaaataaaa aggagagacg
    16081 tacatggaga agaaagaact tttgtatgaa ggaaaagcca aaaaaatata tcttacttct
    16141 gatgagaact tgatttggat agaatattta gatcaagcaa ctgcgttgaa tggcgcgagg
    16201 aaagaccaga taagtggaaa aggagaattg aataatcaaa tcacaagtgc aattttccgt
    16261 tatctgactc aaagaggaat aaaaaatcat tttattgaac aattgtctaa aaccgaacaa
    16321 ttagcaaaaa aagtacaaat tatccctctg gaagtggtta tccgaaatat tgcagcagga
    16381 agtttctcaa aacgtctagg tgttcccgaa ggtaaagaat tcatcaaacc aatcattgaa
    16441 ttctactata aggatgacca gttggatgat ccatttatca atgaagagca tatctatttc
    16501 ttacaacttg caaagccgga agaaatcagt gagctgaaag cccaagcgtt ggctattaat
    16561 gaaattttga ccgaattatt tgctaaggtc ggtttggttt tagtagattt caagctagaa
    16621 tttggccggt cgaatggaca aattttgctt gcggatgaag taacaccgga tacttgccga
    16681 ttatgggatg aacgtacgaa tgaacatatg gacaaagacg tttaccgcag agatctaggt
    16741 gatttgatcc cagtctatca agaagtatat aaccgtctat cggaactaac tgaagaaaag
    16801 gagcgagtat gatgtatttc gtaaaagtat atgtaaccta taaagattca gtgttagatc
    16861 ctcaagggga ggcagtaaaa aaagcagtcc atcgtatggg atatgaaaca atcgaagata
    16921 tccgaatagg aaaatacttt gagatacaac ttgctgatgc agaagaacca atcgatacag
    16981 tgattgaaac gatttgtgac aagctgcttg caaatgtaaa tatggaaaca taccgatatg
    17041 aaatcaaaaa aatggaggaa gtataacatg aaatttgcgg tcatcgtttt tccaggttca
    17101 aattgtgacg cagatatgtt atgggcagtt cgcgagatca tgggcgctga tgccgaattt
    17161 gtacggcatg atgagtcctc tttagaagga tttgatgggg ttttactgcc gggaggattc
    17221 tcctatggtg actatttgag atgcggtgcg attgctcggt tttctacaat catggaagaa
    17281 gtgattcgtt ttgctgaaga agggaaacca gtattcggta catgcaatgg attccagata
    17341 ttgacagaag caggactttt gccaggtgta ctgagacgaa atgagtcttt gcattttatt
    17401 tgcaaaactg taccattgaa agtcaacccc aagacggcct ttacttcaga gtatgaagcc
    17461 gacgaaataa tctatcttcc agttgctcat ggggaaggaa attatttctg tgacgaagaa
    17521 acacttcaag aactaaaaaa aaataatcag attgccttta cttataatga caacatcaac
    17581 ggcagcgtgg aaaacattgc agggattacg aacaaagcag ggaatgtgtt aggaatgatg
    17641 cctcatccag aacgtgcagt agaagacttg ctaggatcag aagatgggaa acggttcttt
    17701 gcttccattt taaaaaactt cggaaaggtg accgtataga tgaaaacatt gaaagaaccg
    17761 acagctgaag agatcaagaa aaaaagaatc tatgccgaat ggggattgac tgatgaagaa
    17821 tatcgaatga tcgaagaaga tatcttagga agaatgccta attatacgga aactggactg
    17881 ttttctgtta tgtggagcga acattgttcc tataaaaatt ctaaaccggt tctacgcaaa
    17941 tttcctacta gtggacctca tgtgttgcaa ggccctggag aaggcgcagg catcgtcgac
    18001 atcggtgatg gactagcagt tgtcttcaaa gcagaaagtc acaatcatcc ttcagccgtg
    18061 gagccttatg aaggagctgc gacaggagtt ggcggaatca tcagagatat tttcagcatg
    18121 ggggctcgtc caattgcgtt gctagattca ttgaggttcg gcgaattaga taatccaaga
    18181 acgaaatatt tactagaaga agtagttgca ggtatcggcg gatacggcaa ttgtatcggc
    18241 ataccgactg taggcggcga aattgcattt gatccttgtt atgaaggaaa cccattagtc
    18301 aatgcaatgt gcgtaggttt gattgaacac aaacatatcc agaaagggca agccgctggc
    18361 gtcggtaatt cgatcatgta tgtaggtgca aaaacaggtc gcgacggtat ccacggagca
    18421 acttttgcct cagaagaatt taatcaagaa gaagaacagc aacgttctgc tgtacaagta
    18481 ggcgatcctt ttatggaaaa gctgctgctt gaagcgtgtt tggaattaat cttgcatcat
    18541 tcggattctc taataggtat acaggatatg ggagcagcag gacttgtttc atctagtgcg
    18601 gaaatggctt caaaagctgg ctcaggactt atattgacat tagatgaagt cccacaaaga
    18661 gaaactggga tgacacctta tgaaatgatg ttatcggaat cacaagaacg gatgttgatt
    18721 tgtgttaaat caggagagga agaaaaaatc caagaactat ttcagaagta tgatctagat
    18781 gcagtcacta tcggtaaagt aacagacgac gggcaatatc ggctatatca tcaaggagaa
    18841 gaagtagcaa atctgccagt cgatgcatta gctgaagatg caccagttta tcataaagaa
    18901 atgaaagaac cagctcgaat cgtagaattt cagcagttag ctccttatca gccagttatc
    18961 gaagaacctg gacaagtatt gcttgatctg ctgcagcaac cgacgattgc ttcaaaacgt
    19021 tcaatctacg aaacttatga ttcacaagta caaaccaata cagttgtccg tccaggaagc
    19081 gatgctgctg tgatgagggt tagagggacg aataaagcgt tagctatgac cacagactgc
    19141 aacgcgcgct atctgtattt ggatccaaaa atcggaggac aaatagcagt ggcagaagct
    19201 gctagaaata ttattgctag cggtggtaag cccttggcga tcaccgactg cctgaactat
    19261 ggttctcctg ataaaccaga agttttttgg gaattatcga cttcagctga cggaattgct
    19321 gctgcgtgtg aaacgttagg aactcctgtt atttcaggaa atgtctcttt atataatgaa
    19381 acggatggtc aagcaatcta tccaacacca atgattggta tggtcggatt gattgaggat
    19441 cataaacata tcactactca ggaattcaaa aaatcagggg atctaatcta tatattaggt
    19501 aagacgtttg cagattttga tggcagtgaa ttacaaaaga tgcagcttgg aagaatcgaa
    19561 ggtgtgatcc gaaactttga tctatccatt gaaaagagaa accaagagtt agtattgaca
    19621 gccatccaaa acggattgat tgaaagtgct catgactgtt cagaaggtgg cttggcaatt
    19681 gcactggcag aatcagcttt caaacatcag ctagggatat ctgtacagtt tgaattgtcc
    19741 tctgctcaat tatttgcaga aactcaatca agatttgtgt tgacagtcgc accggaaaat
    19801 aagacacgat tcgaagaaat gatgggagat gctgctgttt tagcaggtaa ggtgacggat
    19861 gaagcgataa tcgaaatttc agcaactgac ggtcagatca aaatagaaac tgcagtagca
    19921 agaaagtgct gggaggatgc aatcgtatgt cttatgaagt gaagagttta aatgaagaat
    19981 gcgggatctt cggtgtttgg ggtcatccag acgccgctag tgtggcatac ttcgggctcc
    20041 atagtcttca gcatcgagga caagaaggtg caggtatcgt gtccaatgat cgaggaaagc
    20101 ttaaggggca tcgagatttg gggctgcttt ctgaggtatt caaagatcaa agaaaattgc
    20161 agcagttaac aggagaagct gcaatcggac atgtacgtta tgcaacagca ggcaatggaa
    20221 gtgtggacaa tattcagcct tttctattca aatttcatga tcagtcgatc ggcttagcgc
    20281 ataatggtaa cttgatcaat gctcgttccc ttagaaaaga gttagaggaa aatggggcga
    20341 tcttccattc taactccgat acggagatat tgatgcattt gatccgacga agtgaagaaa
    20401 cagatttttt aaataaatta aaaagtgctt tgaatgaagt caaaggtggc tttgcctatt
    20461 tattaatgac agaaacggca atgattgccg ctttggaccc aaatggtttt cgtccattag
    20521 caatcggaca aatgaaaaac ggagcatacg tgattgcctc ggaaacttgt gcattagaag
    20581 ttattggggc acgttttata agagatgtcc agcctggtga gatagtgatc atcaatgacg
    20641 aaggaatcca gattgattgc tttacgaagg acacgcagct ttctatttgt tcgatggaat
    20701 atatctattt tgccagacca gattcaaaca tcgcagggat caatgtgcat actgcacgga
    20761 aaaatatggg acggaattta gcgaaagaat cgccagtaaa agcagatatg gtcgttggcg
    20821 tacctaattc ttctttgtca gccgcaagcg gttatgcgga agctagtgga atcccttatg
    20881 aaatcggatt agtaaaaaat caatacattg cccgaacatt tatccagcca acgcctgagc
    20941 taagagagca aggtgttcga atgaaattat ctgctgtacg aggagtagtg gaaggtaaaa
    21001 aggtgattat ggtcgatgat tcaattgttc ggggtacgac aagcagacgg atcgtccatc
    21061 ttttgaaaga agcaggggca aaagaagtcc atgtgaggat tgcttctcct cctttgaaat
    21121 atccatgttt ttacgggatt gatattcaaa cgcgtaaaga actcattgcg gcaaatcaca
    21181 cgattgatga aatcaaagaa tgtatcggtg cagattcttt aggtttttta agtgaagagg
    21241 gtctgatcga atcgatcggt ctggaaaaag atgctccgta ttcaggattg tgtatggctt
    21301 atttcaacgg agattatccg acgccgttat atgactatga agaaaaatat caggaatcac
    21361 taaaagaaaa agtatcgttc ttttaaaagc agaccaagaa gggagtatat gaattcaatg
    21421 gcaaatgcat acgcaaaagc tggcgtagat gtcgaagctg gatatgaagt agtagaacgg
    21481 atcaagaagc atgtcaaaaa aacggaacgt ttaggtatga tgggcgctat aggaggattc
    21541 ggcggttgtt ttgatttatc ggtctttcaa ttaaaagagc cagtccttgt ctcggggacc
    21601 gatggcgtgg ggacgaaatt agcagtggca atcgaagcag acaaacacga tacaatcggg
    21661 atcgactgcg tagcgatgtg tgtcaatgat atcttagcac aaggcgcaga gccgttatat
    21721 ttcttagact acttagcaac ggggaaaaat gatccgttac gtttggaaaa tgtagtaaaa
    21781 ggtgtagcag atggatgtgt tcaaggaaat atggcgctga ttggcggaga aacagctgaa
    21841 atgcctggta tgtactcagg taatgattat gatctagccg gatttgctgt agggatagct
    21901 gaaaaatctc aattattgac aggagaaacg atcaacccag gagatgtgtt gcttggaata
    21961 gcatctagcg ggattcattc aaatgggtac tctcttgttc gcaaaatttt ctttgagcag
    22021 cagcagtttt ctttatcatc aaaactgcca gaattgaaaa aaacacttgg cgaagaattg
    22081 cttgagccaa cgaaaatcta tgtttcttcc ttgcttccgc tgatcaaaga aaagaaaata
    22141 aaaggagctg cacatatcac tggtggaggg tttgtagaaa atatcccgcg aatgttacca
    22201 gaatctatgg ctgcacaaat caaacttggc tcatggccga ttcttccaat attcggctgt
    22261 ttacaaaaat atggtgaaat tcctgagatg gagatgtatg aaatcttcaa tatgggcatc
    22321 ggtatggtcc tggcagtcga agaatcgaaa gtaaaagaaa tagtacacga aattgagcaa
    22381 gctggcgaaa aagtttatga aatcgggaaa gtgatcgcca aagaaaaaca tcgtgtggtg
    22441 tttataggag aagaacgatg agaatcgctg tttttgcttc tggaaacgga agcaactttc
    22501 aagcgctagc cgattatcta tcgaaaaaag gattggaatc ttcgattgac tggttgtttt
    22561 gtgatcagcc tgaagcatat gttttgaaac gtgcaactgc gttgagtgta ccggcagatt
    22621 gtttttcacc taaagaattt gattcaaaaa aagagtatga agaagcaatt ttgcataaat
    22681 tgaaagaaaa aaagatcgat ttgatcgtat tagctggata tatgcgaatc attggaccgg
    22741 tattgctgaa aaattatgat aaaagaatca tcaatatcca tccgtctctt ttacctgcct
    22801 tccctggttt gcatggcata cgtgatgcct ttgaagctgg agtaaaagaa acgggtgtca
    22861 caattcatta tatcgaccaa ggtgtagata ctggaccaat catccggcag gaaaaagtga
    22921 gaattgaaca ggaggacacg ttcgattctt tagaagaaaa aatccataga gtagagcatc
    22981 gaatctatcc agaagtgatt agtgaaatta ttgaaaatgg aggctatctg aaatgacaag
    23041 agcattgatc agtgtatcag ataaaactgg gattgtagca ttcgcccaag gtttgattga
    23101 acaaggagta gaaatcattt caacaggagg aacaaagcat gcgttggata aagctgggat
    23161 caaaacgctt tcgatcgaag aagtgacagg gtttcctgaa atgatggatg gccgtgtcaa
    23221 aacacttcat cctaaaatcc atggtggtct tttaggaaga cgcgatctcc ctagccatat
    23281 ggaggcaatg gaaacggaaa atatccaacc aatcgacttt gtttgtgtca atttatatcc
    23341 tttcaaagaa acgattatga aaccagatgt cgaaatagca gaagctattg aaaacattga
    23401 tatcggtggt cctagtatgc tccgtagtgc agcaaaaaac catgaatttg tcacctctgt
    23461 tgttgatcca tctgactatg aagaagtact ttcagaatta cgtcagtatg ggcatacttc
    23521 actttctttc agacaaaaac tagcggcaaa agtctttcgt catactgcgg catatgatgc
    23581 actgatcgct caatacttaa cggattgggt ggaagaaaaa gaaccggaaa aattgacact
    23641 tacgtatgaa cgaaaacaaa cgcttcgtta cggtgaaaat agccatcaag ccgctgcctt
    23701 ttatcagtcc gctcttctgg aaaaatattc gatcgcagca gcaaaacaac tccatgggaa
    23761 agaactttct tataataata tccgagatgc agatgctgcg ctacggatca tgaaggaata
    23821 tgacgagcca acggtagtcg ccttgaaaca catgaatcct tgtggtattg ggacagcatc
    23881 tacgatttta gaagcgtggg aagcagcgta tgaagcagat cctgtttcaa tctttggtgg
    23941 gatcatcgtt ttgaatcgag aagtagatct gccaacagca gaacagatgc ataagctgtt
    24001 tctagaaatc atcattgccc cttcttatac acaagaagct ttggaattac tgcaaaaaaa
    24061 gaaaaacatc cgattactga cagtagactt tgcgcaggaa gaaaatcaaa atcgagaaga
    24121 aacggtctca gtcttaggtg gacttcttgt acaagatcaa gatctggcaa tagaaaatga
    24181 agaagagtgg aaagtcgtga caaaacgtaa gccgactcca gcagaagcaa aagcattagt
    24241 gtttgcttgg agagctgtca aacatgtgaa atctaatgcg atcgtactgg caaacgaacg
    24301 tcagacagta ggaatcggag ccgggcagat gaatcgagtc ggttcagtaa aaattgcgat
    24361 tgaacaagcg caagagaaaa tggaaggtgc agttttagct agcgatgctt attttccaat
    24421 ggatgattcg gttgaatatg cagccaaaca tggtataaaa gcaatcattc agccaggagg
    24481 aagcatcaaa gaccaagatt ctattgatat ggcgaataaa tacgatctag caatggtctt
    24541 tactgatgtt cggcatttta gacattagag aggagtcgga taaatgaaca ttttggtgat
    24601 cggtgcaggt ggtcgtgaac atgcaataag taaaaaactt ttagccagtc cgaaggtgga
    24661 tacggtgtat tgtgcgccag gaaatccggg gatgacaaaa gatggtatca attgtgtcgc
    24721 tatcagtgag cgagatcatt cagcgttaat ccattttgtt aagaaagagg ctattgattg
    24781 gagtatcgtt ggtcctgaag ttcctttgtt aaatgggatc gtcgatgatt ttatggcgga
    24841 aggattgaaa atcttcggtc cgacaaaagc agcagctctg atcgaaggtt cgaaaagttt
    24901 tgctaaacaa ttaatggata cttatcaaat cccgacagct aaatccagaa gttttgaatg
    24961 ttacgaaaaa gcagcagcgt atatcaaaga aaatggtgcg ccaatcgtag tgaaagcgga
    25021 cggcttagca gctgggaaag gtgtagtcgt ggctaccaca gtgaaggaag cgctggatgc
    25081 ggcagaacaa atgttgaacc agcatcgatt tggggatagt agcaaaaaaa tcgtcgtaga
    25141 agagtttttg gcaggagaag aattctccct tttggcgttt gtccgtaata ccaaggtgta
    25201 tccgatggtc atttcgcaag atcataagcg ggcatttgat aacgataaag ggccaaatac
    25261 aggcggaatg ggtgcttatt caccagtacc gcaaatttca aaaaagatga ttgaaaaagc
    25321 tgtggaagaa gttttaaaac cagcggcttt gggaatgaaa gaactaggac gctctttcac
    25381 gggtgtttta tatgcgggtt tgattgctac gaaggaagga ccaaaagtga tcgaattcaa
    25441 tgcgcgtttc ggtgatcctg aaacgcaagt cgttctttct cggttgaaga gtgactttgc
    25501 gcaagtaatc gatgatttat tagagaatcg tacagtagag ttaaagtggc agcaagaagg
    25561 atacaatgta ggcgttgtgg ttgcagctgc cggttacccc gaagaatatg agacaggaat
    25621 ggttctacca gattttctgg aagaagagct ttctgtttac tatgctggag tttcctcaaa
    25681 taacggcaaa ctagttggag caggaggacg tctttattta gtagaagcat acggtgagac
    25741 gatcaaagaa gcacagaaaa aaatttacac cgcgttaatg gataaaacga cagatggaac
    25801 attttatcgt acagatattg gagaaaaagc tgccaatagc gaactgatcg tgaagtaaat
    25861 cagctgcttc catttataaa aaatacaaca aacataatcg gaagcgccga ttttagtgaa
    25921 gccttttcat aaagagtgtg gtacactttt gttgtgtata taaataagga ggagttcaga
    25981 tgaaaaaaat tcaattaggt acttctgatc agaaagcagc tgcagtgatc ttaggctgta
    26041 tgcgtatcaa caatgcggaa gatccagtaa aagtgattga gacagcttat gaaaacggca
    26101 ttgatttttt tgaccatgca gatatttatg gaaacggtga atgtgaagaa atctttgcag
    26161 atgctctagc aaaaacatca atcaaacgag aagatctttt tattcaaaca aaatgtggaa
    26221 tcgttccagg tgtgatgttt gatttttcta aaaaacatat cattgaatct gtagaaaaaa
    26281 gtttgacccg tctgaaaatg gattatgtgg atgctttact tcttcatcgt ccagatacat
    26341 tggtagaacc agaggaagta gctgaagcgt ttgatgaatt ggaacggtca ggaaaagttc
    26401 gttattttgg cgtcagcaat caaaaaccaa tgcaaatcga attgctgaaa aaatatgttc
    26461 gtcaaccatt agtagctaat cagctgcaat ttggcttgaa acatactgga atgatcgatc
    26521 aagggattca tgtcaatatg acagatgaag caagtatcga tcatgacggt agtgtacttg
    26581 attattcccg attgcatgat atgacgatcc aagcatggtc accataccag tatggatttt
    26641 ttgaaggtgt atttatcggg aacgaaaaat tccctgaatt aaaccaaaca ttagaagaac
    26701 tagcagctaa atatcaaacg acaccaactg gcttggcttc tgcttggatt ttacgccatc
    26761 cagccaatat gcaagtgatc gccggtacga tgaacgtagg gcgaatcaag gaaatcgctc
    26821 aagcttcaga aattaatatg tctcgggaag attggtacga attgtaccgt gctgctggaa
    26881 acattttacc ttaaagattg tcataagcag gtcaaaaggg ctataaatta aaaagacaga
    26941 ataacttgaa agctgttttc agcttcgaga tattctgtct ttttttattg caaatggaaa
    27001 gagtatcatt gtacggattt cgtttattag ttatttttgt ttttaaggaa aagctgtgtc
    27061 cgatattctt ttggagaaat cccttgtttt ttcttaaata aataactgaa atagttaggg
    27121 tcattataac caatttcaaa agcaatatct gaaatacgat agtctgattg ctgcaataat
    27181 ttttttgcta attcgattct ttgttctgtc agataatcga tgaatgtttg gttaaacgat
    27241 tgagaaaaaa tcgtactgaa atgagaggga cttaatgcaa cttgttgagc gacccaagtt
    27301 aaggacatat ctggatcggt aaaattttct ttgataaaat ccaatgcatg gttcaaaatt
    27361 ggctgatatt tgttccttga aggctgtgag ggcagagcag ttaagaaaga aatgaacata
    27421 taaactgtcg aacggtaaag cgagtcgtcg gcgctgattg ctaagcgttg actggtggat
    27481 gtgcaatgaa ccatttcttg cgggattttc ttttctttca ggcagacatg ctggagtagc
    27541 ttagtcaaga caaataaacg atataatcgt gttcgagttg gtgtgccttg tggtttcgtc
    27601 aagtgttcga tgtacttaga tacttcgttt ttcgataatg aagacaaatc aagtgagagg
    27661 tcaaagtaaa agacagaatc aactggaagt ttcgcttctt gaatcaaatc ctcatagctg
    27721 ataatttgtt ctttaaggga actggcggtt tcaagtatca tctgcgtgat ctcaaaagat
    27781 gcaggtaatt cgctgattct ttctgcgaca ggaccaatgg cgacagaaaa attatttttt
    27841 tgtccctttt ctaattcatg gaccaacgtt tgggctacct gataactggt ctcgatcaca
    27901 ttttcttcaa gagaatcaac tattaaaaat ttgatgaatc gagaggaaac agcagagaag
    27961 atcacttgtt gattctccct aaataaaaac ttcaaataat cactgagatg agcgtaatct
    28021 tcaaagtctg tactgacttg gtttgctgct aataatacgg tcattttttt ccctgctaaa
    28081 gagcgattga aagattcagc aagtttcaat gtttcactga tcgtaagtga gcctttgaac
    28141 aaaccgttca agaaatgatt ttttttcaat tcgaaaacaa gatcagaaga agtgtgcgtt
    28201 tctttttgct gcaaatctaa agcagttact gctttttcta ttgcttgttt caattcgcta
    28261 cttttaatcg gtttaagcag ataatcatct gcctgtacct ggattgctcc tttcatataa
    28321 tcgaaatcgt cgaatccact aataaatatg agacgagtcc aaggaagtag tttccttgct
    28381 tcttttgaaa aagtcaaccc gtccatgaaa ggcatttgga tatcagtcag aatgatgtcg
    28441 ggctgtaagt cgataattga agacagtgcc atctcaccat ctccagcttc gccgacataa
    28501 gtgattggat aataagaacc aaggacagtt atttgttccc gcaagttatt gcgaatgagg
    28561 tattcgtctt cgatgataaa aactttatac atgtttgttt cctccaattg ttttgggaga
    28621 tagagggatc atcagtgtga cagttgttcc ttctcggtat ttgctgtcaa tcgtgatagg
    28681 tttttgatca tttccgtaat aaagaaggag acgcttgttt acgttgtaaa gtccgtagcc
    28741 gatactgaaa tctgaatcga tccctttggc taactcttta cgtatttctg ccagtttttc
    28801 tgcagggatg ccaatcccgt tgtctataac tttgaattgg atcatctcat caattttgca
    28861 tgtggtaata cgaataaatc ctttgtgacg gacgtacttc gttccatggt atacagcatt
    28921 ttcaatcaat ggctgcaaga tcatttttaa aatagaaaca ggaaaaagtg tttctggtat
    28981 atcgatctta tagtcaagca tatggccgta tcttattttt aagatgacta gataatcatt
    29041 gatatggcta atttcttttt caagaggaat ccagtcttgt cctttgctta aagacgtacg
    29101 aaaaaagccg gaaagtgcat gggtcatagt cagagcttct tcaaagcagt cttgttcgat
    29161 caaagaaata atggcatcca aactgttata aataaaatgg ggagtaatct gagcttgcaa
    29221 tacacgaact tcactttgtg ctaaatggta ttgtttcaaa gcgttctccc gtaaagactg
    29281 gtttagatct tccgccattt tattcatgct agcggtaagt gtttgtaatt ccagtgtttc
    29341 cggcagggtt aaacgaaagc caaggttccc tgaagaaagt tgttctgaca tctcgatcag
    29401 ctgattgatc ggttcttgga cctttctctc taaaaagcga cgattctttt tcgcatagaa
    29461 aaaaatggta ctaagaactc caagttccac tattgttaat agaatgagcg agcggacaag
    29521 tgtcgtattt tttttgcttg ctaagtcgat ctccacacga acaaaatctt gtaagatatc
    29581 tgacagcagt tgagtgacag aatctacctg attgatgatc tcttcattgg tatccataaa
    29641 atcttttgcc aagatattat tgatcagctc gttttgataa tcttccagtg tatcgatcgt
    29701 acgtagcgat acatctaacg tactaatttc tttaagtgta tttgtattat ttttgatctc
    29761 tcgaatatca ttcctaagtt cttctaggat attttcctca ttgaactcat ctacggttac
    29821 ttggccagaa atcaagtccc acatactttc caaaactttt tgatcaacct tcaaagcaat
    29881 tgcatctgct ttttggatat ttgcgatcgt ctgttgataa atcaaaagct gttgcgaata
    29941 taacagtatg ctcagtagca aagggataac agcaaataga agcatggctt tgtttgcttg
    30001 ggcaaaaatc tgttttatgc ttaaattttt tgtcatgata cttctccagt caataatttc
    30061 tggttgggat gttagctata ttctgctggg aaaagacagt ctcggaaata aaaatatctt
    30121 taggtaatgt ttttcctgtt tgaatatttc caaaataacg agtaacggta ttggctacat
    30181 accagccgac atcaggttta cactcgacta cacaattgac gattcctgag cgtagcttct
    30241 cgatcatatc ttcttgtcca tcaatagtta caatgatcaa atcttctcca ggtttgattg
    30301 atgtttcttt tatcgcagct aaagcaccgt gagtcatttc atcactatgg ctgtataata
    30361 catcgatttc ggataaatca tttgtttcta aatatcgttt catctggtct tttcctttca
    30421 tccgaataaa atcaccgaca attcggtgaa caatagtcat cggaggttta tggtcgatgg
    30481 ttccatcttt ttttagggtt ggtttacgat tgattgattc caaaaatcct tcggagcgta
    30541 gcttggtcgg tgtggagttc tctgaaccag caagttctag gattctgatt tcctgtttct
    30601 ttgaatttgt gaagtgattg ctgacaaata aacccgcccg atttccttct gctttgaaac
    30661 tggggccaat gtgggtgacg aaaagctcgg gatcattcgt ttgaatatgt ctgtcgacaa
    30721 gtatgacagg gatccctgct cgctttgctt cttttaatac agagtcccac ccggattctt
    30781 gcaaaggagt aaaaatgatc atatccactt tataggcaat gaacgtccgt atatcttgga
    30841 tttgccgttc ctgattcatg tagccatttc ggtagagaac ttggtaatct tctttttcaa
    30901 gcgcagtgat aattgattca gtatgctttt tccgccagct actctccgtt cctgattgcg
    30961 aaaaaccaac gactagtggg tcaggtgctt cctttgtatt aggtgtacac cccatcattt
    31021 ggtaagcgat tacaacaagg gctaaaaaaa ataaaacacg tttcacttta aatcactcca
    31081 tctggatttg ttagtactat cttatcagat aaaagggaaa atcaaagtca aaaggaatca
    31141 atcgaaaaaa atccaagaaa cttaaaaaaa tccaagacaa aaaaagtaca cctaaaaaat
    31201 ttaaagtaaa tcccataaaa aaatcaatgt taacgctttc tttattagct atactcggtg
    31261 tatcaattga aatgggggat aaaaaatgaa aaacaaattt agaaagattg ttgggactat
    31321 cgctattttg accgcaagca ttctattaag tgcgtgtggc aattcaggat ctgcagatga
    31381 tagtacgggg tatgtaggaa tcgctatgcc gaccaaatca gcagaaagat ggatcgctga
    31441 tgggaacaac atggtctcgg aattagaaaa actgggttat aaaacagatc ttcaatatgg
    31501 agaagataaa gtagaaaatc aagtagcaca aatagaaaat atgattacaa aaggggtaga
    31561 tacacttgtg attgcttcta tcgatggttc ggctttaact gatgtgttag ctaaagcaaa
    31621 agaagcagat atcaaggtga tcgcctatga tcgtttactg atgaattcag aaaatgtcga
    31681 ttattacgcg acttttgata attttggagt aggtgtatcg caagcagctt atattgaaga
    31741 gcacttagga ttgaaagaag gaaaaggacc attcacgatt gaattatttg gtggctcgcc
    31801 tgacgacaac aacgcactaa tcaattacaa cggagtgatg tctgttttac aaccatatat
    31861 ggacaacggt cagttggtcg tgccatctgg acagacgagt ttcagtcaaa tcgctacgct
    31921 tcgctgggat ggttctacgg cgcaagcaag gatggataat ctgttaagcg caaattatac
    31981 tgaccaaaca ttggatgctg ttttgtcgcc atatgatcca ataagtttag gaatcatttc
    32041 gtcattaaaa ggcgtggggt atgggagtga aagtaaacct ttacctgtga ttactggaca
    32101 agatgcgaca gttgctggcg ttaaatcgat cattgccggg gaacaaacac aaacgatctt
    32161 taaagacaca cgtatcttag caaaaaatac gattgaaatg atcaaagcaa tcagtgatgg
    32221 agaagaggtt ccggtcaatg atactgaaac ttatgataat ggggtgaaaa ctgtccctac
    32281 gtatttagca aatacagtat ctgtcgataa agataattat caagcagagc tgatcgacac
    32341 cgattattat aaagaatccg atctgaaaaa ttaagaggaa ccaaacaagg aactaaagag
    32401 atctaagtca gagagaggat aagaattatg gcggattgta ttttaaaaat ggcagatatc
    32461 accaaaaaat tttctggaat ccgagcgtta gattgcgtga atctgatgat tgaacgtggg
    32521 gaaatccatg cattgtgcgg agaaaacgga gcgggaaaat caacattgat gaatgtcttg
    32581 agcggcttgt atccatatgg ctcctatgac ggggaaatct tttatgatgg aaagctgtgt
    32641 caatttaaag atttgaaaga tagtgaagaa aaaggcatcg tcatcattca tcaagaatta
    32701 gcactaagtc cttatctttc aataaaagag aatatttttc tcggaaatga acaagcagaa
    32761 agaggcgtga ttgattggga actgacagaa aagaaaacat atgaactgct gaataaagtc
    32821 ggtttgtcta tcgacccgaa tcttctagta tcccagattg gcgtagggca acagcaacta
    32881 gttgaaatag ctaaagcatt atcaaaatcc gtccgtctgt tgattttgga tgagcctacc
    32941 gctgcattga atgaagaaga aagcgcgaat ttgctgagat tgatccgaga gttgaaagag
    33001 caaggtgtaa cctccatcat tatttcacat aagctgaatg aaatcgtgga tatcgcagat
    33061 cgaatcacca ttttacggga tggacagacg attgagacac tagaaaatga aatgatcagc
    33121 gaagagcata ttattcgcgg aatggtgggc agagatttag ttgatcgtta tcctgaaaga
    33181 aaaccggata ttggagaagt ttattttaaa gtagaagact ggacggtcta tcaccctctt
    33241 gatcacgagc gaatcataaa cgaccacatc aacttgcatt tgaaaaaagg agagattgta
    33301 ggaatagctg gactgatggg agcgggcaga actgaattcg ctatgagtct atttggacat
    33361 tcttatggaa gcaagatatc cggacgtata ttaaaggaag gggcagagat atcagttaag
    33421 accgttcctt cagccattgc aaacggactc gcttatgtct cggaggaccg aaaatcttta
    33481 ggactgaatc tgctgatgga tattcgggaa aacgtgacgc tgagcagttt ggataaaata
    33541 agtcaaaaag gagtattaga taaagaaaaa gaagtaactg aggcagaaaa tttccggaaa
    33601 aagatgcgta taaaaacaaa ctccatccat caaattgtca gtagtcttag tggtgggaat
    33661 caacaaaaag tcgttttatc caagtggctg atgacacaac ccgatattct ttttttggat
    33721 gagccaaccc gtgggataga tgttggggca aaatatgaga tctacacgat tatcgaagaa
    33781 atggcagcaa atggcaaagc tgtctgtctt atctcttctg aacttcctga aatattaggg
    33841 atgtgtgatc gaatctatac gatgaacgaa ggacgtatta caggagaagt ctcgcgagaa
    33901 gatgcagatc aggaagtctt gatgaaatta atgacgaaag aagaggtggg ttaagttgga
    33961 acaggcaaaa aatcttacgg ataaaaaaga gaaaaaacta aatttgaaaa gtagagcagc
    34021 tgatcttttt ggtcgctaca gcatggtgat cattttagcg ggactactta ttgcgtttca
    34081 attgatgacg gatggtattt tctggcgtcc attgaatata actaaccttg ttctgcaaaa
    34141 tagccatata ttagtactag ctgcagggat gttattagtt gtgctcttag gtcatgtgga
    34201 tttgtcggta gggtcagtga tggcgtttgt tggtgcaatt gcgggcatga tgatggtcaa
    34261 tcgccaaatg aacccgtgga tgacagttat cgcatgtctt gcagtaggcg ggctgatcgg
    34321 cgcatggcaa ggtttttggg tagcttatgc agggataccg gcgtttatcg tgactttagc
    34381 aggattgctg atgtttcgtg gactgacaca agttgttcta ggaggccaat ctctagcacc
    34441 ttttccagct gttttccaaa aaatgtctac gggttattta ccagacttaa gcgaaggaag
    34501 cagccttcac ttcttgacat tgattttagg aatgatcatc acgatcgtgt tagttgttgg
    34561 tcaatggcga acaagaaatc gtcggaaaaa tcatttattt gaagtggaat ctccaacagc
    34621 tttcttggta aaggcggtat ttacagcagt cgttgtacta ggaataactt atatctttgc
    34681 ctcttatcaa gggtatccga ttattttgat cattcttggt ttgatcgttg cagcatatgg
    34741 gtttttgaca aataaaacag tagcaggtcg acagatttat gcgacgggcg gaaatcgaaa
    34801 agcggctgaa ctatcaggga tcaaaacaaa gcgaatcacg ttctgggtat tcgtcaacat
    34861 gggagtcatg gcagctttag ctggattagt tttagctgct cgattgaatg cggcaacacc
    34921 tcaagcaggt acatcgatgg aactggatgc aatggcggca gtatattttg gtggtgcttc
    34981 gacttccggc ggcattggaa caatcatggg tgcgattgtc ggtggcttgg tcatgggtgt
    35041 attgaacaat ggcatgtcga ttttaggcgt cggggtagac tggcagcagg caatcaaagg
    35101 attgatcttg cttttagccg ttgtgctaga cttgtataat aagaaaaaac gaacaacgta
    35161 ggagaacgcc ggttattcag aaaaaagact gtgacaagac atttgtcaca gtcttttttt
    35221 cgctaaaagc gaacattcat tgtttcgttt gtatagacta aaaataatat atgttttttt
    35281 accattcgta taaattctct ttttgctctt gatttttttg cttaattgag ttaaattaca
    35341 tagggcgcta aaaaaagcct tgattaaatt aataatgttc gtgttttgct ggatgtgaac
    35401 atcaggagga taatgtaatg aaagtatttg attacgaaga tattcaatta attcctaata
    35461 aatgtatcgt aaatagccgt tctgaatgtg atacaacggt cacattaggt aaacatacat
    35521 tcaaaatgcc agtagtgcct gccaacatgc agacgattat tgatgaaaca atcgcagaat
    35581 tcttagcgga aaatggttat ttctatatca tgcatcggtt tgatgaagca gctcgaattc
    35641 cgtttatcaa aaaaatgaaa aagcgtggat tgattacctc gatcagtgtt ggcgtcaaga
    35701 aagaggaata ttcctttata gagaagttgg cagaagaatc gctaaatcct gattatatca
    35761 ctatcgacat cgcgcatgga catgctaact cagttattga tatgattcag catattaaaa
    35821 aatatttgcc tgaaactttt gtgattgccg gtaatgttgg tacaccggaa gccgtccgtg
    35881 aattggaaaa tgcgggcgca gatgcaacaa aagtcgggat tggtccagga aaagtatgta
    35941 ttacaaaaat caagacaggc tttgggactg gaggctggca actggcagct cttagatggt
    36001 gtgcgaaagc agctcgcaag ccgatcatcg ctgacggtgg gatcagaaca catggcgata
    36061 ttgcaaaatc tgtccgtttt ggtgcgacaa tggtcatgat tggttcattg tttgccggtc
    36121 acgaggaatc tcctggcgaa acaaaagtcg aaaatggaat cgtgtataaa gaatactttg
    36181 gttctgcttc agaattccaa aaaggcgaaa aacgaaatgt tgaaggaaag aaaatctgga
    36241 tccaacacaa aggatctttg aaagatacac tagtggaaat gcaacaagac cttcaatcat
    36301 ctatttctta tgctggagga agagatctgg aagccattcg aaaagttgac tatgtcatcg
    36361 tgaaaaattc gatttttaat ggagacacaa tctaaaaaat aaataaaata ttaggatgtt
    36421 gtgaaaaagt tgtcctgcat taagtaataa gaacagccaa acaaaaatag ttctttatat
    36481 gttttgtttg gctggaatta ttatcgcaga tacaagcttt tgaacatcat tattcagaat
    36541 aagggactgt gataagattt ttgtcacagt cccttatgtt ttgcaaaaaa agtttttctt
    36601 ttttctcgta aattcggtaa aatcaaaaga aaaaatagac aatagaaaag agaagcttat
    36661 gtgggaaaaa gtagagaaaa aacaaggacc gatttatcgt caaattatgg aacagattat
    36721 gagagaaatc gaaaacggaa ggatcaatcc tggaagtcaa cttgaatcag aacgaaaact
    36781 agggcaactt tttggtgtga atcgttccac cgttgtccat gcattggaag agcttaggga
    36841 gcttggggta ctaacgagca aacgtggcag cggatggttt gttaatcaga ctgaatgggg
    36901 gaaattcgct gttccaagaa tcgattggcg gcaaatgatc tctcctcgtt atgagcagac
    36961 ggactggtat gagcaaaaaa taagagaagc aaagcagatc caaaaaacat cgtttcttga
    37021 tttatacgct agcgagatgc cagatgagct tttgccgaat tttgagtttc cttcttattc
    37081 tcttgaagaa atcttagcgg aagaaaaaga aatggatctt ttaggctata ctccgttacg
    37141 taacaaaatc aaacggtatt tggaaagtaa atttgaattg gcattttccc ccaaccagct
    37201 tcttgtgact ggtggcggtc agcaagcaat ctttttgatt ttacaaacga ttctttcagc
    37261 tggagagggg atagctgtag agtcaccttc ttttttttac cgattacctc ttttcaaagc
    37321 ttcaggaacg cgtttatttg ggataccgat ggatgaagaa gggatcgact tagaaaaact
    37381 gaaagaatcg atccataaaa ataaattgaa agcagtgctt atcaacccga attttcaaaa
    37441 ccctacagga aaagtgatga gtcaaaaacg acgagaaaag ctagtagaat tgtgtcgaca
    37501 gtatcggctt ccaattattg aagacgatgt atttacggat ctttcatttc taaaccaaga
    37561 agaagtcctt cctatacgaa cgattgatcc ggataatgtg ttatacgtcg gttcgttatc
    37621 tagggtttta ggaaaaacaa cgaaaatcgg ttgggtgata ggacctgaga cttttatctc
    37681 gcaactagct caagcgcaag aaatgatgga gttttcgatg aatatcctta cacaaatcac
    37741 agtggctaat gtatttaatg aagcaattga tttgaaaatg caagatgtaa gaaaaaagtt
    37801 gaaagaaaaa agccgactga taagcgcttg gggagattca caatcgtttt tcaagcttca
    37861 ttcaatttta ggtggttact atgcctggat tacttgggat gggaaaaaac tagaccccga
    37921 actagcagaa aaagtagtag atatgggtct tggaattgca ccaagtctac tttttgggca
    37981 agatataaat ggaatcagaa tcaattacag taggctggat gaaaaccaaa taccgttgtt
    38041 ttattcaaag atgaaaattc tagaaaagtg gttaacagaa taatttcaca aagtaaatag
    38101 attaaagaat ataacagctt ggatttaaat ttcaagctgt ttcttttttt tcggaaaaag
    38161 aatacgatat aaagaaacta agaaaaaacc attatttgta cgtccgttca cgatgtgtaa
    38221 ataaaataaa tgtttgcaac aaaactgtat aacaccaacg tttttaaata gaaacagatg
    38281 gttgtaagtt tgattagaaa atagaaaaaa tgcagatgaa atataaaacg ctttccttat
    38341 tgtgatttta ttcactaatg ctgttaatat gcggattggc ataatttcct ttcatcgtga
    38401 aaatttgtgc ttttagcttg tatttctcat aaatccatga taatttatta atgaagacgg
    38461 ttaaatagtg atacagtttt attcactgtt tcaatcgcag aatgaagaga ttttcttata
    38521 gatgagagag gaatgtgtga caaatggcaa acaaaaaagc accgatcgac ttccaagcac
    38581 tgctagatga agtcaatgca gatttcccag tatatcaagc acttgatcaa gatggaaacg
    38641 tagttaacaa agatttagta cctgatttat ccgatgatga actcgttgaa ttaatgactc
    38701 atatggtatg gtctcgtgta ttagaccaac gttcaacagc gctgaaccgc caaggacgcc
    38761 taggcttttt tgcaccaact gctggacaag aggcaagtca gctggctagt gcttacgcat
    38821 ttgataaaga agatgttctt cttccaggtt atcgggatgt acctcaacta gtaaaacatg
    38881 gtttaccttt atctcaagct tttttatggt ctcgaggaca tgctgcaggt aattcttatc
    38941 cggaagaatt gaaagctcta ccaccacaaa ttatcatcgg tgcacaatat gtacaagcag
    39001 ccggtgttgc attaggtttg aagaaacgaa acaagaaaaa tgtcgtcttc acttatactg
    39061 gtgatggcgg ttcatcacaa ggtgattttt acgaagcaat caatttcgct ggcgcttacc
    39121 atgcaaatgc cgtattttat atccaaaaca acgggtatgc gatctctaca cctcgtgaaa
    39181 aacaaactgc agcaaaaaca cttgcacaaa aagctgtcgc agctgggatt cctggtattc
    39241 aagtcgatgg aatggatccg cttgcagtat atactgtatc taaaatggca cgggaatggg
    39301 cgatcagtgg aaatggacca gtcctaatcg aaacactgac ttatcgttat ggaccacata
    39361 cactatctgg tgacgatcca acccgctacc gtacaaaaga cacagatgac gattggcaga
    39421 aaaaagatcc attgatccgc ttccgtaaat atttgacgga aaaaggactt tggtctgaag
    39481 aaaaagaaga acaagtcatc gaagcaacta aagaagaaat caaagcagct attgctgaag
    39541 ccgacaaagt accaaaacaa aaggtttcag atttcttgaa aaacatgttc gaagtatctc
    39601 cacaatcaat caaagaacaa attgcaattt atgaagcgaa ggagtcgaaa taatcatggc
    39661 acaaaaaaca atgatccaag caatcacaga tgccttagca cttgaactag aaaatgacga
    39721 gaatgtcgtt gtttttggtg aagatgttgg gaaaaacgga ggagttttcc gggcaactga
    39781 aggattgcag gaaaagttcg gagaagaccg agtattcgat actcctttag ctgaatctgg
    39841 aatcgcagga ttgagtttcg gtcttgcgtt agaaggattt cgtccagttc ctgaaatcca
    39901 attctttggt tttattttcg aagcaatgga cgaagtagta gctcaaatgg ctcgtacaag
    39961 atatcgaatg agcggcacta gaaatctacc catcacaatt cgttcgccat ttggtggcgg
    40021 tgttcataca ccagaattgc attcggataa tttagaaggt ttgatcgctc agtctcctgg
    40081 tattcgagta gtgattcctt ccaaccctta tgatgcaaaa ggactattga tttcttctat
    40141 tagaagtaac gatccggttg ttttcctaga acacatgaaa ctatatcgct cattccgaga
    40201 agaagtgccg gatgaagctt acgaagttcc tttggataaa gcagcagtta ctcgtgaggg
    40261 aacagatgta tctattatca catacggagc aatggtgcgt gaagcaatca aagcagcaga
    40321 caatcttgca aaagaaaata tatctgttga aatcattgat cttcgtacag ttgcgccatt
    40381 agatgtagaa acgatcattc aatccgtgga aaaaactggt cgcgtagttg ttgtccaaga
    40441 agcacaacga caagcgggcg ttgccgcgca agtagtttct gagatctcag aacgcgctat
    40501 cctttcatta gaagccccaa tcggacgagt ttctgcacct gatacagtct tcccatttgg
    40561 acaagcagaa aatgtctggt tgccaaatgc aaaagatatc gaagacaaag taaaagaaat
    40621 cgcggaattt taagaaaaga agggacgaaa caaagtcatg gcttatcaat ttaaattgcc
    40681 ggatatcggt gaaggaatcg cagaaggtga aatcgtcaaa tggttcgtca aaccaggaga
    40741 tacgatcaat gaagatgata cattactaga agttcaaaat gacaaatcag tagaagaaat
    40801 tccgtcacct gtcacaggga cagttaaaaa cgtgattgtt ccagaaggca ctgtagcaaa
    40861 tgttggggat gtattagtag aaatcgatgc tcctggacac aatagtgcac cgtcaactag
    40921 tgcgccatca gctgaagcac caaaagaaaa agtggaaaca tcaggttcag caagtgtcgt
    40981 tgaagctgcc gatccaaata aacgtgtatt ggctatgcca tctgttcgtc aatttgcccg
    41041 tgaaaaagat gtagatatct cacaagtaac tgcaactgga aaaggtggca gagtaacaaa
    41101 agaggacatc gaaaacttct tggcaggtgg tccaagcagt gcaccagcga aatcagaagc
    41161 gccagaagcg gcagcaccga aagaagcagc tcctgcagca gaaagcaaac cagctgcacc
    41221 agcaaaacca ttcaagtcca acttaggaga cttggaagaa cgtgtagcaa tgacgccaac
    41281 acgtaaagct atcgctaaag caatggtcaa cagtaagcat acagctccac acgtgacatt
    41341 gcatgatgaa gtagaagtgt ctaaactatg ggataatcgg aaacgattta aagaagttgc
    41401 tgctgcaaat ggtacgaaac ttactttctt gccatatgtc gtgaaagctt taacagcgac
    41461 tgttaagaaa tacccagtat tgaatgcatc tatcgacgat gcaaaccaag aaatcgttta
    41521 taaacattac tacaatatcg gtattgcaac tgatacggat cacggactat atgtaccaaa
    41581 tgtgaaagat gcagaccgca aaggaatgtt tgctattgca gacgaaatca atgagaaagc
    41641 aaaattagct catgacggca aactatctgc agaagatatg cgcaatggaa caattacgat
    41701 cagcaatatc ggatctgtag gtggcggttg gtttacacca gtcatcaact atccagaagt
    41761 agctatttta ggtgtaggaa cgattgcaca acaaccaatc gtaaatgctg aaggcgaaat
    41821 cgttgtcggt cgtgtgatga aactgtcatt aagtttcgat caccgtatcg tagatggagc
    41881 aactgcgcaa caagcaatga ataatatcaa acgtctttta gctgatccag agctattaat
    41941 gatggaagga tgataaacaa atggtagttg gagattttgc tgtagaatta gatacagttg
    42001 taattggtgc gggacctggt ggctatgtag ctgccatccg cgctgctgaa atgggacaaa
    42061 aagtagcaat cattgaaaga gagtatattg gcggtgtttg tttgaatgtc ggctgtattc
    42121 cttcaaaagc actgatagct gcaggacacc attatcaaga gtcactagat tctgaaatgt
    42181 ttggagtaac tagtgaaaac gtcaaactag attttgcaaa aacacaagaa tggaaagaaa
    42241 acaaagttgt ccatacgttg acttctggtg ttggtttcct attaaaaaaa cataaggttg
    42301 aaacaatcga aggagaagcg ttcttcgtag atgatcacac actacgcgtc attcatccag
    42361 attcagctca aacttattcg ttcaataacg caatcatcgc aacaggttcc cgtccaatcg
    42421 agattccagg atttaaattc ggaggacgtg tgttagattc tactggcgga ttagcattga
    42481 aagaagtacc taagaaattt gtgatcatcg gtggcggtgt gattggtgcc gaattgggcg
    42541 gagcgtacgc aaacttagga gcagaagtaa cgattcttga aggctcacca caaattttac
    42601 caacttacga aaaagatcta gtaaaattag tagaagatga tttcaagaaa aaaggcgtaa
    42661 cagtcgtaac gaatgcaatg gcgaaagaag cagtcgataa cggtgacagc gtgacagtga
    42721 aatatgcagt ggacgggaaa gaagaatccg ttactgctga ttatgtgatg gtcactgtag
    42781 gtcgtcgtcc gaatacagat gatatgggct tagaacaagc gggtgtcgaa gtaggagaac
    42841 gaggattgat cacggtggat aaacaaggac gtacgaacgt acctaacatc tatgccatcg
    42901 gtgatatcgt tccaggggcg gcattagcac acaaagccag ttatgaagca aaaatcgctg
    42961 ctgaagcaat ctctggcaaa aaagtagctg tagattataa agcaatgccg gcagtagcct
    43021 tcactgatcc tgaattggct tctgtaggta tgacgatcaa agaagcaaaa gatgctggta
    43081 tcgaagcaaa agcatataaa ttcccatttt cagggaacgg tcgggcatta tcgcttggta
    43141 aaacagaagg attcatccgt ctagttacga cgatcgaaga taacgtgctt attggcgcgc
    43201 aaatcggtgg agtcggcgca agtgatatgg tctctgaatt agctttagca atcgaatcag
    43261 gaatgaatgc tgaagatatc gccttgacga ttcatcctca tccatctctt ggcgaaatcg
    43321 tcatggatgc ttcggaatta gcattaggtt taccaatcca tatttgattt gaataataca
    43381 ggcgaaacgc aactgaaaaa tcagttgcgt tttgtttttt agaattatca ataatattat
    43441 tatgtaaact taaaaatatg acaacttctt ggtttattac cacttcctat gctacactga
    43501 agaggaatat gaaataggag agttgaagat gcaagaatat cagtatccac ttgatttaga
    43561 ttggacaaca gacgaaatgg tcattgtaat gaagatgtgg gaagatctgg aacgtgcgaa
    43621 tgaaaaaggg ataaacaatg aagctttttt gaagacttat cagcagttta aaactgttgt
    43681 caaatccatt ggtgaggaaa aacgattggg acgggaattt gaaaaagctt ccggttattc
    43741 tttatatcgt acagtgcagc aagcaaaaaa aaatccaaac aaaactttga gaatgaacgg
    43801 gtgagataaa tggaacagat gattgatcat atcaagtcct ggcttgaaga agctggtgag
    43861 aaaatcaaat cttctcaaca aacattaaca atctcgcaaa aatcaaatcg aacggatcta
    43921 gtaacaaatg tcgatcgaaa catccagaat tttctaatcg aaaaaatcca ttcctttgat
    43981 ccagaggcga aaatattggc agaagaagaa gggtgtaatc aattatcgga tctcgatgga
    44041 agagtcttta tcatcgatcc tattgatggc accttgaatt ttgtgattga aggggaaaat
    44101 ttttgtatta tgctcgctgt ttatgaatcc ggcgttggaa agcttggttt catttatgac
    44161 gtgatgagaa atgaactttt ttggggtgga aaaggaatag gcgtgtataa aaatgatgag
    44221 ccattgccta gaccagcaaa taaatcctta tcggaaggct tacttggtgt gaatagttat
    44281 cttttcggtc ataataaata tggaatccga gaaattgcgg aaagcagcat ggggacacgt
    44341 atgtacggat gcgctggtct tgaactagtg gcaatcttga aaggaaacca cattgcttat
    44401 atttcaaacc ttagcccttg ggattatgca gcgggaaatg tcttattgga agagtttggt
    44461 atggtaacaa gcggtctttt tggtactcct ttgacatttg aaaaaaggga atactattta
    44521 gcagggactt cttgtgcata tgcagaaatc gcagaccttt tgaaaaaaga aggctgaaaa
    44581 aaaggtagaa atcattcaca aaaatgataa aaacgagcaa aagattaatt ttctcgaaaa
    44641 aatgaaaaca ttttactata tttcatgaaa aaagtctgct ataattagca gtcgagtaaa
    44701 atttgccctg tcataaagaa ccaataaaaa aggagttagt tacattgaat tatagaaacg
    44761 atatccgtaa cgtcgcaatt attgcccacg ttgaccacgg aaaaacaaca ttagttgatg
    44821 aactattaaa acaatcagat acattagacg cacatacgca attgcaagaa cgtgcgatgg
    44881 actcaaatgc gctagaaaaa gaacgtggta tcacgatttt agccaaaaac acagctgtag
    44941 actataaagg tattcgcgta aacatcatgg atactccagg acacgcggac ttcggtggcg
    45001 aagtagaacg gatcatgaaa atggttgacg gcgttgtgtt ggtcgtagat gcgtatgaag
    45061 gaacaatgcc tcaaacacgt tttgtattga aaaaagcact ggaacaacat atcacaccaa
    45121 tcgttgtcgt aaacaagatt gacaaacctt ctgctcgtcc tgagcatgtc gtagatgaag
    45181 ttcttgaact attcatcgaa ttaggagcag atgatgatca gttagacttt cctgtgattt
    45241 atgcatctgc actaaatggt acgtcaagct tgtcagatga tccagcagat caagaaccaa
    45301 ccatggcacc tatttttgat acaattattg aaaaaatccc tgctcctgtc gataacagcg
    45361 acgaaccatt acaattccaa gtttcattac ttgactacaa cgattatgta ggtcgtatcg
    45421 gaatcggacg tgtatttaga ggaacaatca aagtaggaga ccaagtagca ttaattaaat
    45481 tagacggaac agttaagaaa ttccgtgtaa cgaaattatt tggtttcttt ggactaaaac
    45541 gtctagagat tcaagaagca aaagctggag acttgattgc tgtctcaggt atggaagata
    45601 tctttgtcgg tgaaacagtt actcctgtgg atcatcaaga tgcgttacct atccttcaca
    45661 ttgacgaacc aacattgcaa atgactttct tagttaataa ttcaccattt gccggtcgag
    45721 aaggtaaatt cgtaaccgct cgtaagattg aagaacgttt gatggcagaa cttcaaacag
    45781 atgtatcctt acgagttgaa ccaacaaact ctccagatgc atggacagtt tctggtcgtg
    45841 gagaattgca tttgtctatc ttgattgaaa acatgcgccg tgagggatac gaattgcaag
    45901 tatctcgtcc agaagttatt gaaaaagaaa tcgacggtgt aaaatgcgaa ccgtttgaac
    45961 gcgtgcaaat cgatacacca gaagaatata tgggtagtgt tatcgagtcc ttgagcttac
    46021 gtaaaggtga aatgcaagat atggtccata caggaaacgg acaaatccgt ttgacattct
    46081 taacacctgc ccgcggtttg attggttatt caactgaatt cctttcaatg acacgtggtt
    46141 acggaatcat gaaccatact tttgaccaat atctgccaat gcttcctggt caaattggcg
    46201 gacgtcacca aggggcgctt gtttcgatcg atacaggaaa agcaacgact tattctatca
    46261 tgagtatcga agaacgcggt acagtcttcg tagaaccagg tacagaagta tatgaaggaa
    46321 tgattatcgg ggaaaacagc cgtgataatg atttgactgt caacatcacg aaagcaaaac
    46381 aaatgacaaa cgtacggtct gctactaaag accaaacatc tgtcatcaaa aaaccaaaac
    46441 aattgacgtt ggaagaatct ttggaattct tgaatgatga tgaatattgc gaagtaacac
    46501 cagaaagcat tcgcttgaga aaacaaattt tagagaaaaa tgctcgtgaa aaagcatcta
    46561 aaaagaaaaa ataatctgta aaaggatgaa gagagaaacc agcttgttat aaaaaatagc
    46621 tggtttcttt ttttgtccaa ctcttttctg tggagaagtc atctttgtca atggacataa
    46681 attcagaaaa tacgccgaaa ggcgtttaaa agacttttaa aaaacttatt tctagagagt
    46741 ttttgtttca taataagata atgtttcacg aaaggagatc aacttgtcct tcgaagaaaa
    46801 tcgaagtaaa atggcaagag aaaggagcgg gaagatgaac gacttattaa aacaatactt
    46861 tggctatgat gagtttagac ctggtcaaaa agaaattatc cagaaagtca ttgatcagga
    46921 aaatgtatta ggaatcatgc ctaccggaag tgggaaatca atctgttacc agcttccagc
    46981 tttactatta gatgggctta ctgtggttgt gtccccttta atctctttga tgaaggatca
    47041 ggtggacgcg gcgaatcaat taggtatacc cgccactttt atcaatagct cgttagatgg
    47101 ttacgaaaca gctagacgct tccaagaaat cgatagacag cagtaccgtt tactttatat
    47161 cgctcctgaa cgttttatca tgccggattt tatccaagca atgaagcgat ggaacgtccg
    47221 tatgatcgct attgacgagg cacattgtat ctctcagtgg gggcatgatt tccgcccaag
    47281 ttatttacag atggcgaacc agctagatca actgccaaat cgtcctgtaa ttgttgcttt
    47341 gacagctaca gcgactgtgc aagtagctgc tgacataaaa agacttttaa aaattccaga
    47401 aaacaaccat attcagactg gttttgaacg agaaaatcta cgatttcaag taattaaaga
    47461 tcaaaaaaaa gaacaatatc taatcgaata tttaaagatc aacaaaaatc aatctggtat
    47521 catttatgca gcaacgagaa aagaagtaga ccgtctctat catttgctta aaaaattcga
    47581 tttttccgtg ggtagatatc atggtggtct aaatgagaat gagcgaacag aaatgcaaga
    47641 agccttttta tacgatcgct tgcagctgat tgtagcgaca aatgcatttg gtatgggtat
    47701 aaataaaagc aatgtccgtt ttgttatcca ttatcaaatt cctggctctc tagaagcata
    47761 ctatcaggaa gctggtcggg cgggaagaga tggtctttcc agtgaagcca tcctgctttt
    47821 ctctcctcaa gatatccaag tccaaaagtt ttttatccag cagtcccaaa gagaagaagg
    47881 gcaaaagcaa aaagaatatg aaaaattaaa agcgatgaca gaatacgtgt atattgagtc
    47941 ctgtcttcag caatacatcc taaactattt tggcgagaca agcagtccgt gcaaccgttg
    48001 tggtaactgt ttagatgaca gagaaatagt agaagtaacg acagaagcac agatggttct
    48061 ttcttgctta aaacgtatgg gtgagaacta tggtaaacaa atgctgatga aagtcttagc
    48121 aggatcgaaa gaacaaaagc tgcaagcttt aggttttgga catttatcta cctatggact
    48181 gatgaaaaac caatcgcaaa aagaaacgat gcagttaatt gaatatttga tatcgaatgg
    48241 ctatcttctg accatcaatg gtgagtatcc tgttttgaaa gtaacagaaa gagggataca
    48301 agtattgaaa ggacaagaat ctgtttatcg aaaggaaccc aagaaagtcc agcagctatc
    48361 ggatgaagaa accgatacac tatttgaagt gctgcgggaa ctgcgaacgg atctagcatc
    48421 agaagcaggc gttcccccgt atgtcgtatt ttcagattcc acattgaaag aaatgagccg
    48481 aatacgtcca tcaagcagat tggagatgct tcagataaaa ggagttggcc aaagcaagct
    48541 tgataaatat ggcgaagcct ttttatctcg gataaaaaat gcggactcta acgttctgga
    48601 taagtagaaa gaacgaatac gtaaaaatga ataaaaacaa ctggttaaat ctgttttttt
    48661 tgctccttat gaaaatcaaa tatgttataa ttaaggaatt agaatgggag gtaagcacag
    48721 atggattctt tgtcgaaaaa acttgcgatc caagtattaa atgatgaagc agaacggatc
    48781 aaacgattaa tccgaaatca aaacaacagt ctctgtatct cacaatgcaa agccttcgaa
    48841 gaagtggtag atacacagat gtacggcttt tcccggcaag tcggttttgc tgtccgtata
    48901 ggagtaatcg gaaagaacga gggccagctg atgttaagtg agctagaacg agaattgaat
    48961 cagttatata gtgaggttta ccaagaaatt tatgataaaa aagaagttgg caaggaggaa
    49021 tagacttgcc taagaaagtg aaaaaaaggc atctattaga ctatagcatc ttgattccct
    49081 atttaatttt atgtgtattg ggattgatca tggtatatag ttctacgtcg tatctattat
    49141 tagaaaacgg acaaaatccg tccgcatcag tcattaacca gagtattttt tgggtgctta
    49201 gcttgattgc tatcgcacta ctttataaaa tgaaaacaga tgttttgaaa aaccagcgtt
    49261 tgatcatggc agccatcgca gttttgacga ttttgctgct gattgttgtg ttttttggta
    49321 aggagatcaa tggggcgaaa ggttggctgc aaattgcagg attctcgatc caacctgccg
    49381 aatacctaaa aatcatttct atctggtacc tgtctctgac tttatctaaa agacaaaaca
    49441 gtgtacaaaa agattttctt gggactgtga aacgtccact tgcaatggtt atcggactta
    49501 ctgcattagt cgctattctt cccgattttg gtaatgcggc agttatcttt ttgattattt
    49561 tggttctgct tttagccagc ggagtcaatt atgtgtatac tttgattgta ggagtaggag
    49621 gcttttgtct aagtactttt acgatctggc tgatcaatat cacaaacgga aagatcttac
    49681 ccggacgttt acagtatatc tataatcgat ttgctattta ccaaaatcct ttctcagacg
    49741 aattaaataa agggcatcaa ctagtcaacg gctactatgc catgttcaac ggaggactat
    49801 ttggcagggg attaggtaac agtatccaga aaaaaggatt tttacaagaa gcacaaaccg
    49861 attttattta tgcaatcgta gtggaagaac tgggagtgat tatggggatt ttgatattag
    49921 cgttgctatt ctttatgatt gtgcgcattg tccttgtagg tatccgttca aaagatccgt
    49981 tcaattcgct tttgtgcatt gggatcggcg caatgttttt gatccaagta tttgtcaatc
    50041 ttggaggaat tactggcgtt attcctttga cagggatcac gttcccattt ttaagtcaag
    50101 gaggctccag tttgctgatg ctttcgattt gtgtcggctt tgtcctcaac atcagtgcgg
    50161 atgaaaaacg caagagctta ggtttataat aaaaaaagat gtaggagtgg ttcgatgaaa
    50221 aaagttttag ttgctaacag gggtgaaatc gctgtacgtg tatttcgtgc ttgtacagaa
    50281 ttaggaatta aaactgttgg aatctatgca gaagaagatg aatattccgt acaccgtttc
    50341 aaagcggatg aagcatattt agtaggacaa ggaaaaaaac cgattgatgc gtatctagat
    50401 attgaaggga tcatttctat cgcgaaagaa tgcggagctg acgctattca tccgggttac
    50461 ggtcttttgt cggaaaactt gaattttgcg caacggtgcg aagaagaagg catcattttt
    50521 gttggaccca aactgcatca tttagatatt tttggagata aaatcaaagc aaaagctgca
    50581 gctatcgaag cggggattgc ttcgattcca ggcacagatg gaccaatcgc ttctatagac
    50641 gatgccttgg agtttgcgaa acaatacggt tatccgatta tgatcaaggc agctctaggc
    50701 ggcggcggac gcggcatgcg tgtagctcac gatgaaaaga gtgcaagaga gggctacgaa
    50761 cgtgcgaaaa gcgaagcaaa agcagccttt ggtagtgatg aagtgtatgt tgaaaaatac
    50821 atcgctaatc ctaagcatat cgaagtacag attctcgggg atacgcatgg aaacgtcatc
    50881 catctttttg aaagagattg ttctgttcag cgccgtcacc aaaaagtagt agaagtagcg
    50941 ccttgtgtct ctatgaatga acagcaacgt caaaaaatct gccaggcagc tgtccaattg
    51001 atgaaatatg tcgggtatgt caacgcaggt acagtagaat ttcttgttga aggagatgat
    51061 ttttacttta tcgaagtcaa ccctcgcgtc caagtggaac atacgatcac agaaatgatt
    51121 acagatattg atatcgtaac aacgcaactg ttgattgctc aaggtttgga tctgcataaa
    51181 gaaatcggat tgcctcagca agaaggaatt aaattaaatg gttcagccat tcaatgccga
    51241 atcactacag aagacccgtt gaataacttt ttaccagaca cagggaaaat cgatacgtat
    51301 cgttcacctg gtggttttgg cgtacgtctg gatgtcggaa atgcttacgc gggctatgta
    51361 gtgacaccgt attttgattc attgctagta aaagtatgta cgcatggtgc tacctttgaa
    51421 acagccattc agaaaatgga acgttgcttg aaagaattta gaatccgtgg agtgaaaaca
    51481 aacattccat ttatgctcaa tgtcatcaca catcctgaat tccaatcagg aaatgcaaaa
    51541 acaaccttca ttgatagtac agcaacatta tttgaatttc ctcgactacg agaccgcgga
    51601 aacaaaacga tgaaatatat cggtgaaatt actgtgaatg gttttccagg tatcgaaagt
    51661 ggcgaaaaac cattttatga agaaccacgt atgcctaaag atctaatcac aagaactgat
    51721 tacgtaacgg ctaagaatgt attagatgcg aagggtgccg atgctttggt cgaatggatc
    51781 aaaggacagg aaaatttgtt gttgacggat acgacattta gagacgccca tcaaagtctg
    51841 ctagcaactc gtgttcggac aaaggatttc aagcagattg cacgtttgac tggagaaggt
    51901 cttcctgaac tcttttcaag tgagatgtgg gggggagcaa catttgatgt ggcttaccgc
    51961 tttttgaatg aagatccttg gcaacggttg agaaaaattc gttctttaat gcctaatacg
    52021 ttgctgcaaa tgcttttcag aggttcaaat gccgttgggt attctaatta tcctgacaat
    52081 gtattggtgg aatttgtcaa agaagcagca gcgcaaggaa tcgatgtatt tcgaatcttt
    52141 gatagtttga actggactcc acaaatggaa aaaagtatcc aagctgtacg ggatactggt
    52201 aaaatcgcag aggcagctat ttgctatact ggcgatatca acgatccaag tcgggcaaaa
    52261 tacaatgttc agtattataa agacatggca aaggaactag aacaactggg tgcgcatatc
    52321 attgcaatca aagatatggc tgggctgctt aaaccgcagg cagcttatcg tttgattagt
    52381 gaattgaaag aaacgacaga tctgccaatc catctgcata ctcatgatac cagcgggaat
    52441 ggaatcatca cttattctgc tgcttcaaaa gcaggagtag acatcgttga cgtggcaatg
    52501 agtgcaatga gtgggaatac tagtcagcct agcatgagca gcttgtatta tgcactggtc
    52561 aatggtccac ggctgccaga aatcacgatt gaaaatgcac agaaattaaa ccattattgg
    52621 gaagatgtgc gcatgtatta caaaccgttt gaaaacggct tgaatgctcc tgaaacagaa
    52681 gtatatatgc atgaaatgcc aggaggacag tattcgaatc tacagcaaca ggcaaaagct
    52741 gtcggattag gtcatcgctg ggatgaaatc aaacaaatgt atcatactgt caacttgatg
    52801 tttggcgata tcgttaaagt aactccatca tctaaagtag ttggagatat ggcactattt
    52861 atggtccaaa atgatcttac agaagaagat atttatgaaa aaggcgaaac actaagtttc
    52921 cctgaatctg ttgtgacttt cttccaagga gaactagggc aacccgttgg cggatttcct
    52981 aaaaaattgc aaaagattat tcttaaaggc agacctgcat tgaatgaacg tccaggtctt
    53041 ttggctgaat cagtcgactt caacgaagta aaaaaagaat tggcagaaaa aattggctat
    53101 gaaccaaaac aagacgaagt attaagctat ttgatgtatc cgcaagtctt tttggactat
    53161 caaaaagcat ataatcaatt tggcgatgtt acattactgg acacaccaac atttttccaa
    53221 ggtatacgtt tgggtgaaac aatcaatgta caaatcgaac gcggaaaaac gcttatcatt
    53281 cgtttagatg aaatcggaga acccgatatc gaagggaatc gcgtactgtt cttcaatttg
    53341 aatggacaaa gacgtgaaat cactatcaaa gataattcga tcatcagtac ggtccaaacc
    53401 cgattgaaag ctgaaccgac aaaccgagaa caaattggag caacgatgtc tggttctgtt
    53461 ttggatgtat tagtgaaaaa aggcgataac gttaaaaaag gtgatacgtt gatgattact
    53521 gaagcgatga aaatggaaac ggccatcgaa gcacgttttg acggagaagt cgcacatgta
    53581 tatgtgtctt caggagacac tatttcgtct ggtgaccttt tgattgaagt gactgagaaa
    53641 taataagcga aaggtgggga aacatgaaaa gactgggttt gttttggctg attctgctat
    53701 tagttattgt ggcattctac atggaaccag tcttgtttcc gccaaaactt gaagtgaagg
    53761 aaaactctga tcaacaaata tcaaaccaaa tgacaacgtg gaaataccag gagcttaaag
    53821 caactggttt ttctacttat atcggaaaac cgatcgatca gctggaaaag gattttggcc
    53881 aagcctatga tcgattaact agcggatttg gttttgaaac aagatattat gaaaataaag
    53941 ctagtcatct cacatttgaa gcgaacatcc aagacagcag agtcacaact gttaaagtac
    54001 tgcaaacagg gcctgctgat atcgcgccgt tttatttagg gatgacgatg caagatctta
    54061 cgaaaatcac tactatctat actaatttca cttttgagta ccatgataca gaagttggaa
    54121 tcgaactaat ggaagaggat atgaattatc gtccgctaat tgcttttgat aacgacactt
    54181 ttgctatttt attttttgat caaacaacaa acggcctatt ttcagttgtg tatttagata
    54241 aagatagtct tttgaaacta ttgccatatc aagtgtttgg tgaaggcctt ccacattatc
    54301 aagcagataa atcggctgat tgggaatcaa tcaatcgcgt taaagaacga aaaagtacga
    54361 ctttattaaa tatgcttaga agagaagacg aacttcccat atacaatcaa acacttgcct
    54421 ttcaaaacca cagtcagctg ctactacatg attttttgac aaggcctgag gaaatattgt
    54481 cagaggaacg tatggctgat tggcaaaaat cgctaggcag tgctcaaacg acagtgaaat
    54541 tctcactgac gaatgatgaa ttggagaagt taatcagtaa acaaaaatta gaaaatacta
    54601 gtgggctatt tattcatccg gcgattgacg ctactttttc gtttttatat tggtacagcg
    54661 atccttacaa ccatgaccgt ttcatggacc cgggaacgga ttctgtaggc attgcttttt
    54721 ctaaagaaaa tatgttagtc ttattgcaag aagaaaacgg agaaagtagt gatagcagtg
    54781 atcgttaacg aagagctgtt tcaactggag gatcaatgcc tccaagtcgc tgaaatgata
    54841 aaaaagagta aaacgatggc ttcttatctt gaacataaac agtcaatgga agaatctgca
    54901 aaagtttgcc agttgaaaga agcgttcttg tctaagaaag aagcatttga aaatgtagcg
    54961 gcatatggtg agtatgcacc tgattatcgt gaaaaaaaac gagctgttcg aaaagcaaaa
    55021 agacagcttg atatggatga gcatgttgca gctttccgat atagtgaaac acagctgcaa
    55081 agtctgttgg atcaaattgt ccaagtactt gccgaagaag tttctaatga aatcaaagta
    55141 gatgcaggta gcccgttttt tgaaacgaaa aaacactttg gatgcggagg taattgtcat
    55201 gcaattagac aatagtgaat ttgaaataac aaaacgacgc gggctgatcg tatgggtcta
    55261 cagcttaaaa caattaaaga atctgaaaaa gttcggcttg ttgcattatg tctcaagaag
    55321 aatgaaatat gtcgtgcttt acttaaatga agagacattg gaagaaacag aagaacgaat
    55381 ccgtaaatta cattttgttc gaaacgtaga acgttcttac cgaccggata tcgaaatgaa
    55441 ttttgccgaa aaaataggaa acaaaacagt attaaaagac gaaggtttcg aaattgaaga
    55501 gctaaataca gaaatccgat tagctgaaca tctttaaaaa tctcttatga gcagttttta
    55561 aataaaaaat aacttggata acgatcgatt tttgatcatt gtccaagtat ttttttggta
    55621 tactagtacc agtaccattt ctaaggagtg agtagatgcg gataatatca ggaaattatg
    55681 gcgggcgccg tttaaaaagt ttagcagggg cgaacaccag accaactaca gataaagtaa
    55741 aagaatccat attcaatatg attggaccat attttgatgg agagacagta ttggatctgt
    55801 ttgcaggaag cggtggttta gctatcgaag cagtatctag aggctgcagt catgcggtat
    55861 gcgtagacaa aaattaccaa gcgctaaaaa tcatcaaaga aaacatcgaa attacgaaag
    55921 aaccagagaa atttaccaca ataaaacttg atgcggacaa agcaatcgaa cggctagcag
    55981 cggagaaaca acaattcgac tacttgtttc ttgatccgcc atatgcaaaa caaaaaataa
    56041 tcgatcaaat ccataaaatg gaagaactgg gacttttttc taaaaccgca gttatcgtat
    56101 gcgaaacgga taaaacagtc gaattgccag aaactgtggc tggattccat caaataagaa
    56161 aacagattta tggcataacg gccgtaacta tctatagaaa agagggaaat caatgaaacg
    56221 tgcattattc ccaggaagtt ttgatccttt tacaaaaggt cacttagata cggtggaacg
    56281 tgcggctaaa ttattcgatg aggtagtaat tggtgttttt atcaatactt caaagaaaag
    56341 tttgtttccc ccagaagaaa gaatgacatt gatcacaaaa gctgtttctc atcttcctaa
    56401 tgtaaaagtc atgcatcaag aaaatcaatt gacggtagaa acagcaaaag agattggtgc
    56461 agatgcgttg atcagaggca tacgaagtat caaagacttt gaatatgaac gagagattgc
    56521 tcagatgaat catcatctat atgatgaatt ggaaactgtc ttcttattag caaaaccaga
    56581 atatagtcac gtgagttcca gtattttaaa agaagtactg cactttggcg gagatgtgag
    56641 cagctactta ccgccagtga tcaacgaagc tttagctgga aagcgtgaat caaatgaaag
    56701 ctaaacaatg gttgaaaaca gtactgatta tcatatttgg aatgttgatc attggatttg
    56761 tagtagttcc cttgccgtat tatgtagaat caccgggagc cacgatcgat ctgaaagaac
    56821 taattacggt caatgataag gaagataaag gttctgggtc tttctctctt acctctgttg
    56881 gtatcaggca agctacaggt ttcttagctt taaaagctaa aatgacccct tttgaagaat
    56941 tgatcagtga agaagaattg actggtgggg cgacaaatga agaatataat caaatgcaac
    57001 agtattatat ggattcttcc caaaatgcag caatcgaaca agcgttaaaa cttgcagaca
    57061 agccttataa aatggaattc aaaggcgtat atgtcatgag tgtagagaaa acatcgaatt
    57121 tctttggcaa gctttcagtt ggagatacag taacaaaagt agacgggaaa agctttcaaa
    57181 gtacagaagc atttatggat tatgtaaaaa gccaaaaagt tggacaaaca atcgaaatca
    57241 gttatttgca taatggtgaa gaaaaaacag cttctggtga tctgatcgag ttaccaacag
    57301 ataaaaaggc aggaataggt atcacgctta ctgaccatac cgagatcgaa tcagatacag
    57361 atgttcgtat taattctggc tctatcggtg ggccatcagc gggattgatg ttcactttag
    57421 aaatctatga acaagtcact ggtaaaaact tgcgacatgg taagcaaatt gcaggaactg
    57481 gaacgatcaa cagtgaagga gaagttggcc gaatcggagg aatcgataaa aaagtggcaa
    57541 gtgcggataa agctggcgta gagattttct ttgcaccaga tgatacgatt tcagacgatg
    57601 ttaagaaaga atatcctgaa ataaaaacca actacgaaga agcaaaagct gccgctaaaa
    57661 aattgaattc ctcaatgaaa atcgtaccag taaaaacagt acaagaagcg cttgactatc
    57721 tagagaaaat gaaatgaaac taaataatta aagaggggtt gtgacacgag ttctgtcaca
    57781 acccctcttt ttcgtcaaaa atcttcacga taaaaataaa aacaaaagaa aaaattgacg
    57841 taattagatg ttgaagggac gattttgcca gagctcactg tttcttcaat tttaaatgaa
    57901 aggagtaatg aatgattttt actggtcatt ctttgaaaaa agaacaatga tagaaaaaat
    57961 cagcaagaaa atactagctt cacccaaatt gatcatttgg atgtctatga tagttcttat
    58021 cttttgtatc gcaagtggtt ttttattatc aaatttgcta ttttatagag aaacagaaga
    58081 gactgctgca aaagaaatac tttcttcgtt tagttccagt ggagaagatc catcagctga
    58141 aggaaacgat gtagaagagg atgctcgtcc aaaggtcatg tacaccgata tcaaaggaag
    58201 tgtgaaggag cctggtatct attccttttc ttcagaagaa cgggtctatg atgtactaaa
    58261 gcgtgcaggt ggattattag aagaagcgga tagcgatcga atcaactttt ctgcgaagat
    58321 agaagatcag caagtcctat atatccctgc tgtaggtgaa gagctgccag aacatcttga
    58381 tcagttggca tcctcggaag gaaaacagcc tacagcagat tccgagcctc ccaaaataaa
    58441 tatcaataca gccactccat cagaacttca gcagattcct gggatcggat cagtcaaagc
    58501 gcaagaaatc attcgattta gagaagaaaa cggatccttc cagaaagtgg aagacctgca
    58561 ggagatttct ggtatcggtg aaaagacagt tgaaaaacta aaaaacttcg ttacaattaa
    58621 atagaatgat tcaaaatcga ctggcacgag caataaatgg acgctaatgg atactaagaa
    58681 taatccaatt ctaagagctt ggcaggacga ggaggtagta tttttggata ggaaatagct
    58741 tgattgttcg caaagaaaca ttaaaaaaac caatgctgaa tgtctatctc caaaataaaa
    58801 tatctgggat tcatattatg aatacggctg tcagcggcaa taacagtcaa gctctacgag
    58861 aacgctttgc caaagatgta cttagctata cagcagataa agtatttatt ctcattggga
    58921 cgaatgatct agcagagcat aaacaacttt ctaaagaaac gtaccaaaaa atctgcagtg
    58981 gatgatagtt cagctgaaaa aagactatag ttctgatcat gctttcttta tcacgccacc
    59041 agcagtcgat gagacaaaac aaaaatatcg gaataacaag ttgatcaaag aatatatctc
    59101 tgttctcaca gaagtagcaa aacgccatga ttgtgaagta tcgattttta tacggaattt
    59161 cttgaacaaa tggattcgaa gaatttttat aatgggctaa tggatgacgg acttcatttc
    59221 ggcagtgaag ggtaggatct tttgtcagac aggatgattc gttatattta aagaatattt
    59281 tgtttaagca atgagtactt tttaatcaac ttgaaaaaac ggaggaatat aaaacatgtt
    59341 agaaaaatct tcaaaagcat gcgatacgaa taaagaaaaa gttactgata agtcacataa
    59401 tgaacgcatt ccttgggacc agtactttat ggctcaggca gtactgttat ctttacgtag
    59461 tacgtgtaca cgtttagaag taggagcgac actcgttcga gaaaaaagga tcattgctgg
    59521 cggctataat ggagcagtca gtggagatgt ccactgcata gatgaagggt gttacgtcgt
    59581 ggatggacac tgcttgcgaa cgatccatgc agagatgaat gcactcctac agtgtgcaaa
    59641 acttggtatc ccgacggaag gttcagaaat ctatgtcaca cattttccat gtttggcctg
    59701 tacaaaggct ttgttgcaag ctggtgtgaa agggatcaat tatctccatg actatcgaaa
    59761 tgatccttat gcccaagcgt tgatcgaaca attaggtgta tctgtacacc aagttgcttt
    59821 agactcacag catttttcaa aacttcaaaa tgaattaaca ggaaaacatg tctagcgttt
    59881 tagaaaggca tgttcgaaat cggttgattt ttccggcaat tacgtcgatg attatctgtt
    59941 tgttgtatta cactgacaat tggggttatc gtttgcttct tgccggaatt tttttatggt
    60001 tttcgctaaa agaaggacgt tttatcgcgt acttgtgtat tttgtgtagc ataacggtat
    60061 ccatttctta taggctacaa gagtattcat ttttagagga aaaaacaaca tctgcgcaaa
    60121 ttactgtatt tacagatacg attcgtgcaa atggtaattt tttgacaatg gaaggaaggt
    60181 tatcagagaa taaacaaaag ctccagcttt cttatcaagt aaacacagaa gcagaattga
    60241 aaacatggct taacttccga ggaaagggaa tagaattgac tgctgaaggc gagttcatct
    60301 ctccagcaaa aaagcggaat ctttataatt ttgaccaaga agcttacttc aggaaccata
    60361 gaatttctgg acggttccaa attcaaaaga ttgaacagat caccatgcaa aatcactggc
    60421 gaaactggct tcagagaaaa agaggatctt ttatctcctt tgtccaatct gaatttccgc
    60481 aaaaaacaag tgtatatatt aattctttat tatggggcta taaagatgca ggattcagag
    60541 aaccagaaga aatttttagg gagagtggtt tgctgcatct gttcagtcta tcgggtctac
    60601 acatccaatt ttatttaggg tggctgtatt atttatttcg acgtatcgga tggccactac
    60661 acgtcagtat tattccgttg agtctactaa ctatctgtta tgtttctatt gcaggaagtt
    60721 ctatttctgt tttgcgtgct agtttgctgt ttttattgcg attgctagtc aaattgctga
    60781 ataagcatta ttccaccatg gacttatttg cagttgtctt ttggcttctt ttactatttg
    60841 atcctcgatt tctttttcat gcgggtggac agcttagttt tttcatgagt tttttgttta
    60901 tcctgatttc ttttgatgga gattggttga aaaaaacggc ctctcacgtc ctgtttacta
    60961 tgatcacgat gccgttgatt gtatggcatt tttatgagtg gcctatttta ggtagtcttt
    61021 ttacattact gattgcaccg gtattcaagt ttctttttct tcctggtgtg ttatttctca
    61081 cactcttcaa tcgctggctg ccggaaactt tacttttgtt gatagaagag ggattaatct
    61141 tatttgagcg aataatcgat ttttcttcag ggtcgtctct cattatagga cgtttgcccc
    61201 ttgtgctatc tgggatgtat attttcggat tactgatttg tatggatagg ttgaaagaac
    61261 aacctgtgaa acacttgttt tatgttggtc ttttttcttt tatttttttg actttgccat
    61321 ttttacgctt ttcttcaacg atcgctttta tcgatgtcgg tcaaggagac agtatctttt
    61381 tgcaaagtcc ttttcgaaaa gaaacgattt tgatcgatac tggaggtcag ttacactttg
    61441 aaagagaaaa gtgggcgcaa ggtatgatac gtcctcctgc tgaaagaaat atcgttccat
    61501 atctaaaagg tcaggggata agtgtaatcg ataaactcat cttgacacac gacgatacgg
    61561 atcatgtggg agaattaccc acgttatctc agcattttac tatcaaaaaa atttatatcg
    61621 gttggggcgc tggacgaaat gagccattac aaaggcattt atctgaagct caaaaaaatg
    61681 gtactgagat cattgaaatc aaacatgggg acaggatcag cggttattat gatctttatg
    61741 tattgactcc ttttgaaaag ggagagggaa gaaatgaaga ttcgattggt ctatggatgg
    61801 aatataacaa taggcgcttt ttatttttag gagatttgaa tcaagcaatg gaaaaacaat
    61861 tgcttttgat ttatcctaat ctgaaagccg atgtagtcaa attaggtcat cacggtagcc
    61921 gtacgtcaag taacacagat tttctttccc atattgaagc aaaacatggg attatatcct
    61981 gcggcgtaaa taatcgttat ggtcacccgc acagcgaagt attggagaca ctagaagaaa
    62041 atcagataaa aacatggcga acggatcaac aaggaatgat cacctatcgc tggcatccag
    62101 tttttcatcc tcatgggcta gtggaaacaa tgatagacta gtaattctca tctgctcatg
    62161 gtaggataat ttttgaaagt ggtgagtaat ttgagtatac aagcagagtt acaaaaaata
    62221 cgtaaagaaa aacttgcatc ttgttacttg attttaggaa cagaaaagtt tctccaagat
    62281 caagttcgga cagaaattgt caaaaaaatc caattagagg gcgaagatga cttaaatttt
    62341 ttaagttttg acatggaaaa tgcaagttta gatgaggtag tagctgaagc agagacgttg
    62401 ccgtttttcg gagatcaacg gttagtattc gtagagaatc cttatttttt aacaggtgaa
    62461 aaagtaaaca atggaatcga acaaaatact gatttattga cggcttattt gaaagaacca
    62521 ttagaatcga ctgtattagt attctttgcg ccatatgaaa agttggatga acggaaaaaa
    62581 gtaacgaaac aattgaaaaa aacagcaatt atcatcaatg tccaacagtt gaatgaaaaa
    62641 gaagtacgtc aatatctgat gaatacctta gagaattcct caatcaaaat ggaacgaagt
    62701 gcaattgatt tattcttgag gttgaccgac ttagatttat ccaaaatgat gagagagtta
    62761 cagaaattgg tcctttatgg acaaaatcaa cagcaaatca cggtaagaga agtagaagaa
    62821 ttagttccta aaacgttaga acataatatt tttgacatga cccaatatat tttaaaaggg
    62881 aagacggaac aagctttgcg tttatacgaa gatttagtta tgcaagggga agaaacaatc
    62941 aaaattaatg cgattttgct ttctcaatta cgtttatttt tacagacaaa atttcttgta
    63001 aagattggtt atcagcaggc aaatattgct gaaacattga aaatccatcc atatcgtgta
    63061 aaacttgcca tgcaggaagt aaaaaaattc gatgaacatt tgctggtaca gttgtttgat
    63121 caattagttg agatggatta tcaaatcaaa acgggacaaa tcgaaaaaga attaagtttt
    63181 caactattcg ttttgcgcac agggcagttg ttaagaaatc gataagaaga aagacgaggg
    63241 caaagcatgc gggaaggtcg tttgtttttg tccttttttt gacaaataaa aaaatcggcc
    63301 gaagccgatt cttttattta gcgatttttt tgcttaaacg agatttgtcg cggttagctt
    63361 tgtttttgtg gattagacct tttgattcag ccatatcaat agctttaaca gcttctttat
    63421 ataacgcatc tacgttgtca gcacctgaag ctacagcatc ttcaaatttt ttgattgcag
    63481 tacgcatagc gcttgtttga gatgaatttt tcacattagc gttttcgctt gtacgaacac
    63541 gtttgattgc agattcaatg tttggcatta gtttcacctc cgataactta agttcttacg
    63601 cgtgttaccg cataattcaa cattcattat tatacctaaa ggcagcgtgc tttgcaatat
    63661 gaacatgaca agtttttttc cttaacagtc ttttattagg attttttttc agtaaaaacg
    63721 gatagtacaa gctgaaaaaa atcaaaaaga cgctattgtg ggaggcaata gcgtcaaacc
    63781 aaactagatt tttatttatc tatttaaaca aagtttgaat gggaatgttt ggtactaagt
    63841 tattatataa caaagagaac cgtttgcaaa gttttttttg aaaagggata cgattccctg
    63901 ttgttctttg tttttctatt aagaaactgc agttatttta taaccagtat cctttgatca
    63961 gtaaaggcta ttctggtgta ccgattattt taatgtattc atcgatattt tttatcaaca
    64021 ttttttttat gaccaactgg ttccgacggt tccatttgcc acttacttcc agcacatatt
    64081 tatttagggg caaacgtaag atatattttg ccaaatagcg atctttaagc aggccattga
    64141 tcacacggtc ctcaagctgc aagttgatcc cagtcagcat cattggctgt tcttgtaaaa
    64201 cgtacgtatc aattaaaaga ccttcaaaag tttccacaag cgtcttcctt tcttttcaaa
    64261 gtaacctgaa aacaggcact aattcttcca ttttgatatt cgttgaagtg gatgcgatca
    64321 ttctttcttt tgtccactgt aatctagtat aacaaaaaag agcagaagag ccaaaaagaa
    64381 aaaagagcat cgaattctat aggaagtctc attggcacgg cctaaattat tacaaaacat
    64441 tgattaaaaa aagcccctgc atcgcaggag catcccctat atcgttttaa taggtcaatc
    64501 aaaaagcagt atcaaacaca ttttgtatca aatcaggaaa taatagataa gctaagccca
    64561 gaatcaaaag taccgctaga agaaaccagc cgattgttcc tatgaaatac tttaattttt
    64621 cgaacattgt atcacctcgt tgtcagtata tcacggatca gtaaattggg ggtgttaaga
    64681 atctcctaaa atagacaaaa tttgttctat tttcctaaag caaatacttg taagatacag
    64741 taagtgatac cagaagcaat gaccggtccg gcggcgattc ctcttaagaa gacaacacct
    64801 aaaatcgtgc cgaaaacaag tgcaacagtg atttgtggat cagaagcaat caatcctaca
    64861 cccttggaag ataagacagc cactagccca ccgcaaaaaa cagctaccca gccaataggg
    64921 gatttaaagg cttctaatag atcacgtaga ccgatttctc ctgttgcgat agggattaga
    64981 atagccgctg tgatgatcgt caccccaata ttgattcctt tgccttgaag ccaagggaaa
    65041 aaggaacggg aggctggtat gagcttcata atcaaaacaa aagctgccgc tatcatcaga
    65101 ctttgatttt tagctaaaat tgctacaagt aaaataattc ctaaaaaaag ccaactttcc
    65161 atgaaatata ttcctcactt tcttcgaaac aatcataaca tgtttttaga aagttacaaa
    65221 agtaggaatt catccttcaa gacctattca taagctgttt ttttcataaa ataaacaaaa
    65281 tcataaaaaa acactttgct tatttgacag aagacagccg cgctttgtat agtggacaag
    65341 acttacattt ttttgaagaa tcagaaagat caggtaaagg tcttagtaaa cgcaagccat
    65401 tcagaataac caatattgtg cttccttcgt gtcctacaac tccaagaggt agatttaaaa
    65461 attgcaggaa gttagaagca atcaacaaga gaataacaag aatcgaaaaa ataatatttt
    65521 gtttgatgat ccgccgcatt tttaaagata gccgatgact gtagacaaat ttatttaagt
    65581 cgcttttcat taagacgatg tctgcgatat ccatagcggc atctgtccct tgccccatag
    65641 cgacacctaa agaagcggct gctaaagcag gcgcatcatt gactccatca ccaaccatcg
    65701 catttacttg gtaaagttca ttctcttttt tgactagttg tgttttttct tccggagtac
    65761 agcttgcata gtaatgttca atccctactt gttcagcaat ggcctttgct gtgccagaat
    65821 ggtcgccggt tatcatactt gtatggatgt tttgggattt aaagtaggaa attacttgtt
    65881 gggtatttgc ttttggaata tctagcagac ctaaaacagc aattaactgc tgatctttag
    65941 aaagataaat cacggttttg ccttgatttt ccaaacgttc gatcatttcc gcaatctctt
    66001 tgctgattat catcgtctca ggatcatatg cgtgcttgcc aactttccat tgcgcaccta
    66061 aataggtagt ttgtaatcca aaaccagtaa tttcttctat tggtaagtat tggatttctt
    66121 caggtattgt gcaatcgaaa tattgcaaaa tcgcatgagc aagtggatgc gtcgtttttt
    66181 gttccatagc aacaagaagt tgttgtgcca aatccttttc atcaaagaaa aagctgtcag
    66241 ttacaactgg ttttccttta gtgagcgttc ctgttttatc aaaggcaatc gcttttaatt
    66301 cagaaagctt ttctaaatgg ataccgcctt tgattaaaat cccatttttt gcagaatttg
    66361 aaagagcagc tagagaagct ggtgtagcag atgcgacaag cgcacaagga gaagccacaa
    66421 ctaataatac cattcctcga taaaagcttt catcccaact ccaacctagg aaaaagtggg
    66481 ggagaaggat cattagagga acagcaagca aaatcaattt tacataatta ttttcgattc
    66541 gttcgataaa tgaagctgtt tgagttggcg tattctgcgc ttcttctact aattggataa
    66601 tcttagcaaa gacagtttcg gaactcgttt ttgttactcg aatcgtgatg ggatttcctt
    66661 gattcagcgt tcctgcatac atctcatcgc ctaaatattt ttctacaggc acagattcac
    66721 cgcttaatgc agactcatcg atcgttgcct gtgtgctttc taaaaagcca tctatcggaa
    66781 cagctgctcc tttagcgaca aataccaagt tatccacctt tagttgagaa acatctactt
    66841 cttgcatttg tccattttct tttaataatt gggctttttg tggttgaaga ttcatcaaag
    66901 cagtaatttc tttttgactt ttattagttg tgtattcctc taatgcaccg cttaaacaaa
    66961 agatgaatgt caacattgct ccttcaaagt aattaccgat caaacaggca ccaatagcag
    67021 ctaaagccat caataaatcc acattcagtg tccgatcatt ccacaattct ttcaagcctt
    67081 ccgatgtttg tttatatcca ccgatgataa tggcactgat ataaaaaagt ggggtgatgg
    67141 taagagagaa tcggtcgaag ataaaaccga agatcataaa taaaaggcag ccgattgttg
    67201 ctgcaaaagc ttttttctga ttagtcatcg ttattagacc tccttgtcaa aacaaggata
    67261 gcataataaa aatgtaattg ataataataa cagctaaatg agaacgataa tggttatcat
    67321 ttggtgatta agcttgatgt tgtttttatt atttataatg attattatta aagaaaataa
    67381 aaaaccagaa catttctcaa ataagagaag tgttctggtt ttttctacat tgcaataaac
    67441 caataaccaa tcaaaatgat accaaggata atacgatacc agccaaaagc ggtaaagtcg
    67501 tttttcttca aatagcttag taagaatttg atagctaaga cggaaaccac aaaagatacg
    67561 aatgttccta caagaagaac aacagtttct tccattttga atgaattccc ttcagctaaa
    67621 tatttgacga tcttcaagcc gcttgcgcca aacatgacag gaatccctaa gaaaaatgaa
    67681 aactctgtag cgacaaaacg cgaagcacca atcaagatag atcctaggat cgttgcgcca
    67741 gaacgggatg ttccgggaac aagtgccaag acttggaata acccaacaag aagagctgct
    67801 tggtacgtaa agtcattcag agaagtccaa cgaggtgttt tgtttttgtt gcgtttttcg
    67861 acaataataa aggcaatacc gtaaacgatc aaaacaatgg caactggtaa gaacttgtgg
    67921 aaatttgctt ctaaccaatc atccaaaggc aaaccaatga tgatggctgg tatgcaagca
    67981 acgatcactt taaaccaaag tgtccacgta tctttttttt ctaccgttga tttacttgga
    68041 gaaaatgggt ttaacttgtg aaaataaaga acgacgacag ccaagatcgc ccctaattgg
    68101 ataacgacat tgaacatcga cataaattgt gaactggcat ccagtttgat gaattcatct
    68161 gctaggatca aatgaccagt actactgatg ggcagccatt ccgttattcc ttcaataata
    68221 ccgagaatca ctgctttgat aatatttgca aaaaacataa attccttcct tcccaattaa
    68281 gatacatctt atagtatacc gttatttttt gagaaaccca atctgttttt taatattaag
    68341 aaaaacttga gaaataaagc agataagtac acgaagagaa aaaagaagac aaagccctcg
    68401 ttcagacttt gccttttat
//
LOCUS       DACEKH010000003        63422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEKH010000003 DACEKH010000000
VERSION     DACEKH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.003"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            83..970
                     /locus_tag="IW662_000134"
     CDS             83..970
                     /locus_tag="IW662_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0864989.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IW662_000135"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IW662_000135"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ0864990.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IW662_000136"
     CDS             2272..2718
                     /locus_tag="IW662_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0864991.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IW662_000137"
     CDS             2991..4343
                     /locus_tag="IW662_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ0864992.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IW662_000138"
     CDS             4514..4801
                     /locus_tag="IW662_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0864993.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="IW662_000139"
     CDS             5027..5470
                     /locus_tag="IW662_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0864994.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="IW662_000140"
     CDS             complement(5476..6660)
                     /locus_tag="IW662_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ0864995.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="IW662_000141"
     CDS             6928..7626
                     /locus_tag="IW662_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ0864996.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="IW662_000142"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="IW662_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ0864997.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="IW662_000143"
     CDS             complement(8436..8969)
                     /locus_tag="IW662_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0864998.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="IW662_000144"
     CDS             complement(9266..11647)
                     /locus_tag="IW662_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0864999.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IW662_000145"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IW662_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ0865000.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="IW662_000146"
     CDS             12348..12887
                     /locus_tag="IW662_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ0865001.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="IW662_000147"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="IW662_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ0865002.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="IW662_000148"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IW662_000148"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="IW662_000149"
     CDS             13994..15160
                     /locus_tag="IW662_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ0865003.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="IW662_000150"
     CDS             15206..16702
                     /locus_tag="IW662_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ0865004.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="IW662_000151"
     CDS             16783..17466
                     /locus_tag="IW662_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0865005.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18111..19301
                     /locus_tag="IW662_000152"
     CDS             18111..19301
                     /locus_tag="IW662_000152"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ0865006.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19403..20356
                     /locus_tag="IW662_000153"
     CDS             19403..20356
                     /locus_tag="IW662_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ0865007.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20425..21774
                     /locus_tag="IW662_000154"
     CDS             20425..21774
                     /locus_tag="IW662_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0865008.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21957..22280)
                     /locus_tag="IW662_000155"
     CDS             complement(21957..22280)
                     /locus_tag="IW662_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865009.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22409..23572
                     /locus_tag="IW662_000156"
     CDS             22409..23572
                     /locus_tag="IW662_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865010.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23642..24991
                     /locus_tag="IW662_000157"
     CDS             23642..24991
                     /locus_tag="IW662_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0865011.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25036..25467)
                     /locus_tag="IW662_000158"
     CDS             complement(25036..25467)
                     /locus_tag="IW662_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0865012.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25855..28497
                     /gene="alaS"
                     /locus_tag="IW662_000159"
     CDS             25855..28497
                     /gene="alaS"
                     /locus_tag="IW662_000159"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ0865013.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28697..29293
                     /locus_tag="IW662_000160"
     CDS             28697..29293
                     /locus_tag="IW662_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0865014.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29415..30563
                     /locus_tag="IW662_000161"
     CDS             29415..30563
                     /locus_tag="IW662_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0865015.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30601..31014)
                     /locus_tag="IW662_000162"
     CDS             complement(30601..31014)
                     /locus_tag="IW662_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ0865016.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31093..31524
                     /locus_tag="IW662_000163"
     CDS             31093..31524
                     /locus_tag="IW662_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ0865017.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31623..31856)
                     /locus_tag="IW662_000164"
     CDS             complement(31623..31856)
                     /locus_tag="IW662_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ0865018.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32039..33709
                     /locus_tag="IW662_000165"
     CDS             32039..33709
                     /locus_tag="IW662_000165"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ0865019.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33856..34242
                     /locus_tag="IW662_000166"
     CDS             33856..34242
                     /locus_tag="IW662_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865020.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34229..34927
                     /locus_tag="IW662_000167"
     CDS             34229..34927
                     /locus_tag="IW662_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0865021.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34932..35396
                     /locus_tag="IW662_000168"
     CDS             34932..35396
                     /locus_tag="IW662_000168"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ0865022.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35393..36304
                     /locus_tag="IW662_000169"
     CDS             35393..36304
                     /locus_tag="IW662_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0865023.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36539..37078
                     /gene="pyrR"
                     /locus_tag="IW662_000170"
     CDS             36539..37078
                     /gene="pyrR"
                     /locus_tag="IW662_000170"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ0865024.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37092..38375
                     /locus_tag="IW662_000171"
     CDS             37092..38375
                     /locus_tag="IW662_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ0865025.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38388..39314
                     /locus_tag="IW662_000172"
     CDS             38388..39314
                     /locus_tag="IW662_000172"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ0865026.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39333..40613
                     /locus_tag="IW662_000173"
     CDS             39333..40613
                     /locus_tag="IW662_000173"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ0865027.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40618..41694
                     /gene="carA"
                     /locus_tag="IW662_000174"
     CDS             40618..41694
                     /gene="carA"
                     /locus_tag="IW662_000174"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ0865028.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41699..44884
                     /gene="carB"
                     /locus_tag="IW662_000175"
     CDS             41699..44884
                     /gene="carB"
                     /locus_tag="IW662_000175"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ0865029.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44938..45714
                     /locus_tag="IW662_000176"
     CDS             44938..45714
                     /locus_tag="IW662_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ0865030.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45711..46643
                     /locus_tag="IW662_000177"
     CDS             45711..46643
                     /locus_tag="IW662_000177"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ0865031.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46640..47356
                     /gene="pyrF"
                     /locus_tag="IW662_000178"
     CDS             46640..47356
                     /gene="pyrF"
                     /locus_tag="IW662_000178"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ0865032.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47363..47992
                     /locus_tag="IW662_000179"
     CDS             47363..47992
                     /locus_tag="IW662_000179"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ0865033.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47980..48294
                     /locus_tag="IW662_000180"
     CDS             47980..48294
                     /locus_tag="IW662_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865034.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48332..48973
                     /locus_tag="IW662_000181"
     CDS             48332..48973
                     /locus_tag="IW662_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0865035.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49004..49708
                     /locus_tag="IW662_000182"
     CDS             49004..49708
                     /locus_tag="IW662_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ0865036.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49705..50607
                     /locus_tag="IW662_000183"
     CDS             49705..50607
                     /locus_tag="IW662_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0865037.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50765..52471)
                     /locus_tag="IW662_000184"
     CDS             complement(50765..52471)
                     /locus_tag="IW662_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ0865038.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52661..53284
                     /locus_tag="IW662_000185"
     CDS             52661..53284
                     /locus_tag="IW662_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865039.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53734..54783
                     /locus_tag="IW662_000186"
     CDS             53734..54783
                     /locus_tag="IW662_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0865040.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54780..55661
                     /locus_tag="IW662_000187"
     CDS             54780..55661
                     /locus_tag="IW662_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0865041.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55664..56434
                     /locus_tag="IW662_000188"
     CDS             55664..56434
                     /locus_tag="IW662_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0865042.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56713..57567
                     /gene="aroE"
                     /locus_tag="IW662_000189"
     CDS             56713..57567
                     /gene="aroE"
                     /locus_tag="IW662_000189"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ0865043.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57745..58908
                     /locus_tag="IW662_000190"
     CDS             57745..58908
                     /locus_tag="IW662_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0865044.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59025..59435
                     /locus_tag="IW662_000191"
     CDS             59025..59435
                     /locus_tag="IW662_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0865045.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59609..61837
                     /gene="topB"
                     /locus_tag="IW662_000192"
     CDS             59609..61837
                     /gene="topB"
                     /locus_tag="IW662_000192"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0865046.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            61975..62292
                     /locus_tag="IW662_000193"
     CDS             61975..62292
                     /locus_tag="IW662_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865047.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            62452..62688
                     /locus_tag="IW662_000194"
     CDS             62452..62688
                     /locus_tag="IW662_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0865048.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62685..63083
                     /locus_tag="IW662_000195"
     CDS             62685..63083
                     /locus_tag="IW662_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0865049.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT        20995 a        10951 c        13567 g        17909 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaat cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
    17581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
    17641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
    17701 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
    17761 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
    17821 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
    17881 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
    17941 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
    18001 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
    18061 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
    18121 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
    18181 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
    18241 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
    18301 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
    18361 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
    18421 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
    18481 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
    18541 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
    18601 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
    18661 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
    18721 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
    18781 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga
    18841 aagctatagg tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga
    18901 aagctagaag tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg
    18961 cattggcttc gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa
    19021 atgctaatta ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa
    19081 cacctattac tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc
    19141 tgatcgaaca tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag
    19201 gcaggatcag aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag
    19261 cagtttatca aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt
    19321 ctatttaaaa ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta
    19381 aaaaagatca gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat
    19441 tggttcagag ttgatcacac ggctacgtca agaactaggc agtgaaaatg tcattgcgac
    19501 agatatacgc atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat
    19561 ggattacgaa aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt
    19621 ggctgctctt ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt
    19681 gactgggctg gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc
    19741 aagttcaatc ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct
    19801 tcagcgtcct acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta
    19861 ttattatgtg aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta
    19921 tgcaaccttg ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat
    19981 caaagaagga aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat
    20041 gccagatgcg atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca
    20101 tcgaaatgcc ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat
    20161 tcaaaaaatc ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat
    20221 agcagaatct tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc
    20281 gccgaaatac gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt
    20341 ggtagagacg gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg
    20401 ctatggtatg aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta
    20461 aaaatagaaa atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg
    20521 ggcacagtga tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga
    20581 tccgccagtt tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga
    20641 ctaacaggag ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc
    20701 gaacggattt acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat
    20761 tttccagcaa atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt
    20821 ggtctttccc agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg
    20881 atcaattttt taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag
    20941 cttattcctt tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc
    21001 caactgtttc cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc
    21061 ttgctagcaa cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa
    21121 ttgaaaaagc cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg
    21181 atcgtttatt tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca
    21241 ggaaacagca atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga
    21301 ctggtcacag ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact
    21361 ggaatgcgtc tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt
    21421 gctaaacttc atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc
    21481 gctattggca tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc
    21541 atttggattt tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct
    21601 gatttgtttc gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt
    21661 ggagggactt ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg
    21721 atagtattga cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa
    21781 cattcgaatt ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg
    21841 agtacgtgga agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt
    21901 ctacgatgaa atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac
    21961 tttgatttgc gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag
    22021 caaccagaaa tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac
    22081 aaagcatgtg gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg
    22141 aagactagca tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt
    22201 ctttttctta cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa
    22261 acaaagtaca tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa
    22321 gaaatgagaa ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga
    22381 gagatacaaa gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc
    22441 gctgattgtt tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata
    22501 tcgaaagtat ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta
    22561 tttattgtgt gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca
    22621 aatgacaggg ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa
    22681 cacggtattg gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt
    22741 tttggaacca gcttttaatg ttttattact cataccattt ggcatttatt taagatatta
    22801 tttccggctt tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga
    22861 attgacgcag ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt
    22921 gaatgattta gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt
    22981 tacatttatg cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac
    23041 tgtctcattg actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat
    23101 taatttggta gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca
    23161 tatctggtgg cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa
    23221 tggatggaca cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg
    23281 aatttcttta aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt
    23341 aagctggttg gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca
    23401 gatgagtgca gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa
    23461 tatcttgcta tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac
    23521 atatactgtt agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa
    23581 aaacgctttg tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg
    23641 aatgaataat tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa
    23701 gggcttcaaa cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa
    23761 aaatatcatt gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt
    23821 gtttgaaaaa atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag
    23881 ggaactagct gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga
    23941 gatccgtgtc tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca
    24001 tcaccaacag ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga
    24061 acaagcgctg aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt
    24121 ggatatgggt tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca
    24181 gtttttagtt ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat
    24241 ggaaaatcca atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa
    24301 ttggctgatt tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat
    24361 tggtcatcct tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc
    24421 cgattacttg aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag
    24481 agagagaaaa cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac
    24541 tgatttagct gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat
    24601 tccagaagat ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa
    24661 aggaacggcc attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa
    24721 attgggcgtc tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga
    24781 tcgtaataga cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg
    24841 tcttgtgaag aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc
    24901 aattgcagaa aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc
    24961 tcgtaaaaat aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt
    25021 cacaatctct tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc
    25081 aataaaaggt tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat
    25141 ttcagctact acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc
    25201 aagcaactga ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg
    25261 cttgaagcaa gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc
    25321 aagaaataga taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg
    25381 aatcccttgt tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca
    25441 ttcatccgct ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt
    25501 attgattcct ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa
    25561 ataaaaattg agatattgac agtgatcaat agaatgcgta tactaaataa gtacgaaaag
    25621 gatatgattt gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc
    25681 actaatcaaa ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat
    25741 cgagtttaag aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt
    25801 ccttggctgt gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa
    25861 caattaagta gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaaggacat
    25921 acgattgaac ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac
    25981 tctggtgttg caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga
    26041 atcacaaatg cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca
    26101 cggcaccata cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat
    26161 gaagccatcc actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca
    26221 gaaaaacttt atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa
    26281 gaagttggcc tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt
    26341 gcaggcccat gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat
    26401 ttagcagaag acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc
    26461 tggaacttag tattttcaga attcaaccat aagcctgata acacatttga accacttcct
    26521 cataaaaaca ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg
    26581 tcaacgaact ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc
    26641 aaacaagtaa aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat
    26701 catattcgcg ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga
    26761 gggtatgtct tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt
    26821 gaacaagcat tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat
    26881 cctgaagtac ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt
    26941 ttcaatgaaa caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa
    27001 aacgaaaacg ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc
    27061 ttcccggtag aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct
    27121 ggttttgaaa aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa
    27181 aaatcaatgg gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt
    27241 ggatacacag aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt
    27301 ttgaatgaag tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca
    27361 gaaatgggtg ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta
    27421 gcagttgtta cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta
    27481 aaagcaccaa tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat
    27541 cggatcatca aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt
    27601 ggcgagcatg caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt
    27661 acccactttg gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa
    27721 aaaatctggg aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac
    27781 atgggtgcaa tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt
    27841 ggcggctggt caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga
    27901 atcttcaaaa tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta
    27961 acaagtaaag aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca
    28021 acaattgtta aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag
    28081 caagtaagag agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa
    28141 gctggtgatg tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa
    28201 gtaaaagtga aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat
    28261 atttcagata ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca
    28321 atgacgaaag aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca
    28381 ccgaaagtag gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat
    28441 cctgctggta ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc
    28501 gtttacgttt gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg
    28561 aagacaaaca agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg
    28621 tcggattcgg atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat
    28681 aaaaaggggt gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat
    28741 cttgttagca atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg
    28801 attcattagt ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc
    28861 aaaagatggc atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc
    28921 catgccaaca acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc
    28981 cagagtactt gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa
    29041 atcaataacg atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt
    29101 agtgttagga ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga
    29161 ctctgctctt ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc
    29221 aataggtgcg ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt
    29281 gaaaccagaa taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga
    29341 agagaggaga ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag
    29401 aaggggtaaa aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc
    29461 ggacaaaaaa aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg
    29521 tctctaagct aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga
    29581 acagtctatc agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa
    29641 agccagcaga tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat
    29701 ttgtttcgat catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa
    29761 tcatccatcc agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca
    29821 tctttttgaa atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg
    29881 aaacattgag agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat
    29941 tgctttcagc agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg
    30001 ctttagcatt atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac
    30061 tattaggaag tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt
    30121 gcgatgatat acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct
    30181 ttgcatcagt ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta
    30241 ttgatctgat agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg
    30301 atccaatgcc tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc
    30361 ttcttgaaac aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa
    30421 cgctgcaatt tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa
    30481 gaatcttacg tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg
    30541 atcacaatta tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat
    30601 ttaaagagtc tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc
    30661 tcctttattt ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag
    30721 ttcttggatt ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac
    30781 cgtagaaact agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt
    30841 tttctttaga aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc
    30901 tatagaaagc gggatagata gctgttcttg tttaccttga tagacaatca agattccacc
    30961 tccactagga ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag
    31021 agattctcct ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg
    31081 agaggttaga gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
    31141 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
    31201 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt
    31261 ttgcggcgtt cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg
    31321 aaagaccgag aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg
    31381 caaatgatgt tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa
    31441 gtagtccttt ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct
    31501 tatacgaaaa aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac
    31561 aggaaaagaa aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt
    31621 atctattcat gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct
    31681 tgtgttcctc gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt
    31741 ttgaatccct cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa
    31801 ctgataaagc cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca
    31861 ctcacgacct ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt
    31921 caaataaatt aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg
    31981 gcatatctta acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat
    32041 gaagaatgat ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag
    32101 taaattagat cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa
    32161 tttttctgga atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc
    32221 catcaatcca acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc
    32281 gctcaataaa atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt
    32341 gatgggaatc aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga
    32401 tatcaacttg cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc
    32461 tgctctttta gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt
    32521 gatttggaaa cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt
    32581 gggtgggcca atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga
    32641 aatcatggct atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag
    32701 gatcgttgtc ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga
    32761 aggcgctctg gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta
    32821 cggaacacca gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag
    32881 tattttagct accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt
    32941 tggtgcagat cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc
    33001 gccggatgct gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa
    33061 aaaagatgaa cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa
    33121 gcgccatata cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt
    33181 cgtaacagat acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat
    33241 tcttgccaag agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa
    33301 agctgtagta cgattgattg atagaaaaga agctaattac aagccattat accgcctaga
    33361 agaaacgata caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt
    33421 tgttttcagc aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa
    33481 actgccgatt tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg
    33541 tgcaccggaa gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt
    33601 tttagtagcc ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc
    33661 attgaatatg gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata
    33721 atctaccatc gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt
    33781 aattttgttt acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca
    33841 taataggagg gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt
    33901 agctcgattc agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag
    33961 ataacgcttg atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc
    34021 cccgtttata cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt
    34081 cttcaaatct atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat
    34141 ttatttttca gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac
    34201 cgggcatatt tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt
    34261 caatcgaatc gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac
    34321 cgaacttgtc agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga
    34381 tcttttacag cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt
    34441 tgtcattgca gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact
    34501 gattcagcct gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga
    34561 tcttccgtta cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt
    34621 atacaataaa ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc
    34681 aaaagaaagc caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat
    34741 ccaaaacgga aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt
    34801 tgaagcgatg tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa
    34861 tcccgatggt aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa
    34921 ggattgattt tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc
    34981 agttggtcaa atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac
    35041 caggtatttt gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag
    35101 ggaagatgtg gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga
    35161 tgattaaaaa tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag
    35221 ctggtgcgat tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc
    35281 agacagattt tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag
    35341 tgatttgtat gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga
    35401 acttaaaacg acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt
    35461 caccgattac agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa
    35521 tggacagcca gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat
    35581 ccccgaacca gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga
    35641 agatgaagat gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg
    35701 tcatacgcat ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat
    35761 caacggcgtt attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct
    35821 aatggttgcc aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac
    35881 ctctttgcgc aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat
    35941 caatgcgcca atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg
    36001 gaagtcggct gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga
    36061 attaaaacta gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta
    36121 tccgttagct ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt
    36181 tctccatgcg caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga
    36241 agcacctctt ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt
    36301 ttaatttaga taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt
    36361 taagtttagt cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa
    36421 gtagattgct cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca
    36481 ttcctcctgt cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat
    36541 gcaagcaaaa gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta
    36601 tgagattatc gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg
    36661 aggaatctac attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat
    36721 tcctgtagga gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga
    36781 accggaactt cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat
    36841 tgatgatgtt ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt
    36901 tggcagacca agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc
    36961 aatccgtgca gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt
    37021 agagatgcaa gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag
    37081 gagagagaaa catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc
    37141 acgatcgccc aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt
    37201 tcggagcaac cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca
    37261 gttcaggact aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact
    37321 taggaagcag cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc
    37381 cagcgattgc acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca
    37441 tcaaaaagat cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag
    37501 tcatcatggt tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc
    37561 agggcacgta tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt
    37621 tcttcaacat gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta
    37681 gcggatacat cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg
    37741 cctggtttgc tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc
    37801 ttggagcaat cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc
    37861 atcttatggt attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata
    37921 agacgttgac aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg
    37981 taacaagtta cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg
    38041 tgatcggtgg agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt
    38101 tgattttaag tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa
    38161 ttgccgcaag tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa
    38221 atctgttgat tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag
    38281 gcacatttac attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta
    38341 tcttaccaga aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat
    38401 cagaacgtat cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta
    38461 tgggcttgat ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc
    38521 gccagtactt tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg
    38581 atgtagcgga gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg
    38641 tccaaaaagg ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg
    38701 tagcggttat ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa
    38761 tccagtgttc gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc
    38821 ttgatttgat gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg
    38881 taggtgatat cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag
    38941 gcgcacaagt attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg
    39001 gacattatat gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg
    39061 tgcaacacga acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac
    39121 atgggttaac agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag
    39181 caccagttaa ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga
    39241 tcgttcagca aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa
    39301 atggaaaagc gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga
    39361 tcaatacacg aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca
    39421 aagcaatcgg tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac
    39481 aattgatcac acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat
    39541 acaaggagac gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat
    39601 gcgcgatgcc gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg
    39661 acatcattag aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc
    39721 tgcgaagtga gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca
    39781 caaatgacgg tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg
    39841 caaaaaacaa taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg
    39901 ttatgcacgc tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg
    39961 agtcttctca aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg
    40021 tttgccatgt ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa
    40081 tccacgtaac agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg
    40141 aagatttcgg cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt
    40201 tgatcgaagg cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg
    40261 gattagaaga aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa
    40321 cagcgtttca actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag
    40381 tgatcaattg gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa
    40441 cggttggtgc ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata
    40501 aagaagactt tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg
    40561 aaacacaaat gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg
    40621 gaacgtttgc ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat
    40681 gctagtgtct tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc
    40741 actgatcaaa gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat
    40801 ggggtaaata gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa
    40861 gaacatgcac gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa
    40921 caaaaaggaa ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt
    40981 gaagtcggaa cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt
    41041 gatcagctga aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa
    41101 ccttatccaa gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat
    41161 agtattttac gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca
    41221 gatgccgaga cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt
    41281 gatccaaaag atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg
    41341 atttttggta tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat
    41401 aaaatgaaat tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga
    41461 attgatttta cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa
    41521 aaactcatcg taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga
    41581 gattatcccg ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca
    41641 ctacatttat ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat
    41701 gccgaaacgt acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg
    41761 tcaagccgca gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg
    41821 atatgaagtg gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc
    41881 agatcaagta tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga
    41941 gcgtccagat gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga
    42001 gctggcagca tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc
    42061 tgcgatcgat caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca
    42121 gccaatcccc gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa
    42181 aatcggctat cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat
    42241 gtgtgaaaat gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc
    42301 tacccaatgt ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat
    42361 gagagattcg gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg
    42421 tatccataca ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata
    42481 tcaaatgctt cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg
    42541 taatgtccaa ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc
    42601 gcgtgtttct cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct
    42661 agctgctaaa atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac
    42721 gacttttgca gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc
    42781 gtttgataag tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga
    42841 agtgatggct atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga
    42901 aatcggtaca caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat
    42961 ccaaaaagtg gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag
    43021 agggtacacc atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa
    43081 actgttgcat atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat
    43141 attgaaaaca gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat
    43201 aaacgaagaa gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat
    43261 ggttgacact tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga
    43321 atatgaaaat gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc
    43381 tatccgtatc ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat
    43441 ccagcaggca ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga
    43501 cttttctatt tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt
    43561 gatcgagcta gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa
    43621 cttagccgaa cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt
    43681 ggatcgtgcg gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca
    43741 acctttaggg gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg
    43801 ttatccagtt cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga
    43861 aaaccagcga gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc
    43921 agtactagtg gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg
    43981 tgaaacagta ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg
    44041 ggattcaatg gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga
    44101 agattacacg atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt
    44161 tgtcatccat gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt
    44221 tccattctta agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt
    44281 gggagagaaa ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt
    44341 tcatgttaaa gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg
    44401 gcctgaaatg aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt
    44461 gtacaaggca ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac
    44521 gattgcggac gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg
    44581 ctatagtctg ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt
    44641 tgaaacagta gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag
    44701 agatggtaaa acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa
    44761 agacggtttc atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt
    44821 agacacagca gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat
    44881 ttaacggcga gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg
    44941 aaacaagaaa tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg
    45001 acattgaaag gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc
    45061 gtgccgcgtg cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa
    45121 acaaatactt gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact
    45181 ctttctgccg gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc
    45241 aatcttcata caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta
    45301 tatgagttat caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt
    45361 gccacacatg aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc
    45421 gctactgacg atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg
    45481 aagacggaac ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa
    45541 cagctgtttt cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg
    45601 ataggggctt gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt
    45661 gtgaaagttt gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga
    45721 atcctttagc ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg
    45781 gctgtttcgg ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga
    45841 tcatggtcaa ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg
    45901 aaacaccatc tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta
    45961 tgcataaact tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg
    46021 ctggtgcttg tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg
    46081 tgaaagcaat cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg
    46141 gaacagattc tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc
    46201 ctgtctatgt caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg
    46261 aagctggagg ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc
    46321 ttaagacaag aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca
    46381 agcctgtcgc tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg
    46441 ggatgggcgg cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg
    46501 ctgtagctat agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag
    46561 agctccctgt gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag
    46621 tgagagagga acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt
    46681 ttcaactagg caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt
    46741 tgtgaaaatc ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa
    46801 agaggctggt cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga
    46861 aaaagcaatg cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg
    46921 cggtattcgt atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg
    46981 aaaacgtccg ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca
    47041 tgccgatcaa ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc
    47101 caaaaaagca ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga
    47161 aatggcagga gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc
    47221 aggagatcag acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat
    47281 cgttataggt cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca
    47341 aacagaatgg agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga
    47401 aattgaagca gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag
    47461 cccaatttac tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc
    47521 aaaaggatta gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac
    47581 tgcaacagca ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt
    47641 ctatattcga agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt
    47701 tgaaggtcag aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga
    47761 agcagctgaa gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac
    47821 gtatgagttg ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt
    47881 gaccaattat tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga
    47941 attaactctg ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt
    48001 gatgaagata gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc
    48061 caaaaactga ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat
    48121 cagtttttga tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata
    48181 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
    48241 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
    48301 gaaaaagaaa tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt
    48361 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
    48421 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
    48481 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
    48541 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
    48601 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
    48661 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
    48721 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
    48781 caaaaataac ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa
    48841 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
    48901 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
    48961 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
    49021 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
    49081 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
    49141 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
    49201 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
    49261 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
    49321 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
    49381 agttgtgggt gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa
    49441 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
    49501 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
    49561 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt
    49621 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
    49681 ttattatttc gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta
    49741 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
    49801 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat
    49861 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
    49921 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
    49981 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
    50041 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
    50101 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
    50161 atcagaagat atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg
    50221 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
    50281 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
    50341 gaagacctta tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact
    50401 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
    50461 gggattcgcg agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
    50521 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
    50581 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
    50641 cgttgatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
    50701 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
    50761 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
    50821 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
    50881 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
    50941 ctaactcggc agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt
    51001 gcgaaccggg tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg
    51061 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
    51121 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
    51181 ggtatccttc tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt
    51241 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
    51301 cgccaatcag cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat
    51361 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
    51421 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
    51481 gttcgccatt tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
    51541 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
    51601 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
    51661 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
    51721 gtgtgaaaaa atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
    51781 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
    51841 tcagctcttg agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat
    51901 ctagttcagt ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca
    51961 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
    52021 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
    52081 agacgatcgt actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac
    52141 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
    52201 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
    52261 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
    52321 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
    52381 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
    52441 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
    52501 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc
    52561 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
    52621 ttaggtataa taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt
    52681 agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg cagaaaatcc
    52741 aagtaagtat tttcgacgtc ttgtttttct gctcatctta tcattgctgt taacagttgc
    52801 aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg tttattcgtc
    52861 cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga aaatcaaaca
    52921 aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc aggacatgct
    52981 tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct tttattatcc
    53041 gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg atgaagtgac
    53101 atttattttc tttgatggag gaaaagaagc aaatcggata actgcgggta agggaggaat
    53161 aactgatttt gataaggagt tgatccctga actgaatcga cttccgttag ccaatatcaa
    53221 acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg agattgctaa
    53281 ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa ttatcagaac
    53341 aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt tgacgaaatt
    53401 caaaaattcg attagactta aatcaattct taaggaaagg gtgacctgta atgaacttat
    53461 caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga taagttgtat
    53521 taatggtgag actttcccat agatacaact ttttagagtg catttctaaa gcttgtatct
    53581 atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt tttgtgtaaa
    53641 gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc tgattataat
    53701 aaataaaaag attgactttg caggaggaaa aaaatgaaaa acaaacgatt aataacagtt
    53761 gtagtaatta ttttagttta tttagcaggt gcatttattt atggaaaagc ctcaggcgaa
    53821 aaagaaagta cttcgacgaa ccagacaaaa aaaacaacag ttgttggggt attgcaatac
    53881 gtgagccatc cggcattaga tgagatttat cgaggaatca aagatggtct gaaagaagaa
    53941 ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa atggacaagc cgatcagagc
    54001 aagctagcca caatgagtca acagctagta caatctgatc cagatgcctt gattggaatc
    54061 gcaacacctg ccgctcaatc attagcaaac acgacttcat cgatcccagt tgtgttaggt
    54121 gctgtaacgg atcctgtggg ggctggatta gtcgcttctt tagagaaacc tggtggtaat
    54181 gtaacaggtg tttctgatca gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg
    54241 ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt cagaggataa ttcaaaatac
    54301 caagtagcac aagcagaaga agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg
    54361 gtgccttcca ctaatgagat tgcacaaacg gttcaagtga tgagccgaca agtcgatttt
    54421 atctatgttc cattagataa taccattgcc aatgcgatgc aaacagtggt aaaagaagca
    54481 aacaaagcaa atatccccgt cattccatct gtggatacaa tggtggaaca aggtggacta
    54541 gcaacaatcg ggatcaatca atatcaatta gggcttcaaa gcggtaagat ggcggcagaa
    54601 cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt atacatttga tcaaggggat
    54661 acagtgatca atcaatctca agctgatcgc ttaggaatca ttgttcccca atcgatgaag
    54721 gaaaaagcca aaatcattac agatgactcg cagcaagaaa catctaagga ggatgacaaa
    54781 tgatcgtatc agctatttca caaggcatgc tttgggcaat attaggatta gggattttca
    54841 tgacttatcg gatcttagac tttcctgata tgacgacaga agggtccttc ccattaggtg
    54901 ctgctgtctg tgtgacagct attactcatg gggtagcgcc gattgtagct acattattag
    54961 gcgtaggtgc tggcatgata gcaggactcg ttacggggtt actttataca aaaggaaaga
    55021 taccggttat tttagcaggg attttagtga tgtcaggatt gaactcagtt attttgtttg
    55081 tgatgcgaac accaaatctt tctttattga atcatccgat tctacaagac agcttccatc
    55141 gctttgggtt acctgattac tttgacacag tccttcttgg cttcatctct ttgggcatct
    55201 tgattgctgt actgctattc ttcttcaata cagatttggg tcaaggctat attgcgacag
    55261 gagacaacga aacgatggct cggtcgttag ggataaaaac cgatcgtatg aaaattttag
    55321 gtttaacttt gtcgaacggt gtcattgcat tatccggtgc gctgattgcc cagaacgacg
    55381 gatatgcaga tgtaaataaa gggattggcg taatcgtcat cggtcttgct tccatcatca
    55441 tcggtgaagt gatcttccaa gaattgacgc tagcagaaag attgattgct attgttgtcg
    55501 gcagtatcat ttatcagcta ttgattttag tagtaatcaa actagggttt gacacgactt
    55561 atctgaagtt gttctccgct atcatcttag ctatttgtct gatgatcccg caattgaaga
    55621 aagcactgaa cttaaaaacg ggttttgaga aggaggcata gtaatgactg atatattgac
    55681 aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat gaagagaaag tactattgga
    55741 tgaaattaac cttacaatca gagaagggga cttcatcact gtccttggcg ggaatggtgc
    55801 tggaaaaagc acactgttca atacgatcgc tggtacgctt caactcagtc aaggatcgat
    55861 ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa aaaagagcac gattcctttc
    55921 tcgagttttc caagacccca agatgggaac ggctcctcgg atgacagtag cagaaaattt
    55981 gctacttgca caaaaaagag gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca
    56041 aaaagaagat tttttcagac tatgtcaaga agtcggtaat ggcctagagc aacacttgga
    56101 tactccagcg ggtgaattat ctggtggtca acgccaagca ttaagcttgc tgatggcgac
    56161 gatcacagct cctgagttac tattattaga tgaacatact gcagctttag atcctaaaac
    56221 agctaaagca ttaatgcgat taacaaatca gcggatcaat gaacaaaaat tgacttgtct
    56281 catgatcacg catcgaatgg aagacgcctt gaaatacgga aatcgtctga tcgtactcca
    56341 aaaagggaaa atcgttcaag atatttctga aaaagaaaag caacatttga caatgggcga
    56401 ccttttgcag ttctttgaag aagtagtgga ttgatttcga cagaaaaatt cctttcagta
    56461 gaaggacttg aaaactcatt gacatcttcc tttgattcat cctatactta aaacaataaa
    56521 caattaaaca tatttgggtg ttttgtagga tcacgtagtc aatgatttta acaattaaat
    56581 agaatattct ttgctctgac aagtaaacga tgagaaaaga atttgctact ttgccaccta
    56641 gacgtttcgt actttttcgt atcaaacgct aagtggcttt ttttattgaa gtaagaacaa
    56701 aggaggaata agatgatttc tggacaaacc aaattagctg ggctctttgc tgatccggct
    56761 tctcacagtt tatccccatt gatgcacaat actgcttttg aagcaaacaa catcgatgcg
    56821 gtgtatttag ctttccaagt aaatccaatg aatttgaagc aagcagtcga aagtattcga
    56881 acttttgata tgttaggtgt aaatctttcg atgccgaata aaacagcggt tattccttat
    56941 ctggatgagg taagccagga agcgcaattg atcggagcag tgaatacgat cgtgcacgaa
    57001 aagggtcgtt tgattggcta taatactgat ggtgcgggct tcatgcaatc cgtgcaagaa
    57061 gctgggattt cgatcaaaaa gaaaaaaatg actctactgg gagctggagg tgcagcaaag
    57121 gcagtcactg ttcaagcagc gttagacggc gctgaggaaa tcgtagttta taaacgaaac
    57181 aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt
    57241 aagataaaag ttcgtgactt tgccgatacg catcaattaa gaaaagactt gaaagaaagc
    57301 tgtcttctag tcaatgcaac agatatcggg atgggaaaga aaatcaatca gtcaccgctc
    57361 ttagatataa gcatgatgca tgagaatcta gcggtttttg atttgattta tgcacctaga
    57421 gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc
    57481 atgttgatct accaaggagc aatcgcgttt gaattatgga caaaggaaaa gatgccgatc
    57541 catgtgattg aaaagctgtt tgattaaaaa aggataatag ataaagatct tttattctcg
    57601 cataaacggt ctcaagaaaa gtaatccttt atgtatcgaa gcatttattt cgttattttt
    57661 tattaaatta tcaaaaaatg ataggctttt gtcgattggt tatcgtatcc tttatttatc
    57721 agtaagaaac gaggaggaac cacaatgagt cggatggatc gctataagga catccataag
    57781 aaggcgaaac ctctgaagcg ggatacgggg agtatttttg ctagaagaga acgaaaacga
    57841 acgcgtatgg aaaatgagga agatgctacc cgtatttatg aaagagagca gcattcattt
    57901 gaacatttga accaatacga gcaacagata tacagagatg caccaagagc agaagaacaa
    57961 caaaaaaata aaaaattttt caatcggcaa aagaagaaca aaccgttaaa acctcgaaga
    58021 aagcgatcgt ggaaaaaaat cattctatcg attttgcttg ttttgatcct ttattcaggt
    58081 atctcatttt ttattggtaa atatgtagct gaacatgaca caagcttaga ggcagcgact
    58141 tcagaaacct ttcatgggca aaaagcgacg aatggtgcgc ataatatttt gatccttgga
    58201 agtgatacac gcggagaaga tgccgggcga gccgatacga tcatggtctt acagttggat
    58261 ggtccttccc ataagccgaa actgatttct tttatgcgtg acagcttcgt tacgatccca
    58321 ggggtaggac aaaataagat caattcagct tatgcatacg gaggagccga tctagtgcgt
    58381 caaactttag tagaaaattt tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa
    58441 tcgtttgaaa aagtgatcga tgccttattc atgaacggag tgaagatcga tgcagaaaaa
    58501 gatctgaatc tggatggtgt agatattaaa aaagggggcc agaagatgga tggacatgtt
    58561 cttttgcagt acgcacgttt ccgaatggat gaacaaggag actttggccg tgtcagacgc
    58621 caacagcaag taatgaacgc tattttcagc caattgaaga atccattgaa tctgatccgt
    58681 gcaccttatg cagctggtaa agctttagga tatacatcca cagatgtcac tgcttggttc
    58741 ttgacgaaaa acctcttttc gattgcacga ggagctggtg gcattgatcg aataagtgtg
    58801 ccagtagacg gttcgtggaa ttatgggcaa agtgattatg ctggcagtat tcttgaaatt
    58861 gataatcaaa tgaatcaatc tgctattaca gattttttga ataaatgatc ataaaaaaga
    58921 tcaagaagaa gctgcgcaag gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa
    58981 acgctttctt atggtagatt taaccaagag aggagctgtt tacattggat atctatctga
    59041 acttcaaaaa tgagtcgcgt gaagccatcg ctttttatga aaaagttttt cagactgaag
    59101 catcaggtat caccacatac ggtgaaatgc ctggaataga acaaatgaac ttgccagaag
    59161 aagcaaaaaa tctaatcacg aatgcacggc tgaacattta tgatacatta gttatgtttg
    59221 cagatacgcc agaaggaatt ggtcctgaat tagttgtggg aaataatgtt tctttagtca
    59281 tccagacaag aaatgaagaa gaaacgaagc gcctatttga aagcttagct gagggtggta
    59341 agattgagat gccactggat gctgttccgt gggctcagtt gtatgggatg gtaactgatc
    59401 gatttggcat caactggcag attaaccaag agtgacagct aaagatgaat attttctgga
    59461 aacatcaagc agcaaacgga tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac
    59521 tatacggtat ctttttttta ttgccagatg cgaaaatatg ttccataata gagaagtccg
    59581 caaaaaatta agtaacggag gtcagaagat gaagacggtg atactagctg aaaaaccttc
    59641 acaagcaaaa gcctatgccg aaaccttcca acagcaaaag cggcatgaag ggtattatga
    59701 aatcagcgat ccattgtttg ctggtgaagt aaccatcact tatggttttg gacatttggt
    59761 agatatggtt gctccaggag cttatgaaga gcgttgggca aaatggtctt tagaaaattt
    59821 gcctattttc ccagaaacct tccgttacga agttccaatg gacaaacgtg cacaattttc
    59881 gatcgtaaaa cgagaattgc agtcagccga taccatcatt attgcaacag atggtgatcg
    59941 agaaggtgaa gccatcgcgt ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa
    60001 gaactattta cgtttatgga tcaattctct tgaaaaagaa gcaatctatg aaggttttaa
    60061 gaatctccaa cctggaaaga actattttcc aaaatataaa gaagcgcaag caaggcagaa
    60121 cgctgattgg ctgattggaa tgaatggaag tccattatat agcttgctac ttcaacgaaa
    60181 agggattgat ggcagttttt ctcttggtcg tgtacaaaca ccgacactct atatgattta
    60241 tcagctgcaa gaagaaatca aacacttcaa aaaagaaacg tactttgaag gagaaggttt
    60301 aattacgaca aacaaagggc agttttctgg aaaaatcgta ccaaaaaaag catttaaaac
    60361 ccaagaagag ttaacgaagg aaatagaaaa acttggagcg catttaggta agcaaaacgg
    60421 acggattctt gaagttacga aaaaggaaaa gaggttgcat agtccacgtt tattttcgct
    60481 atctagtttg cagacaaaga tgaatcagct aatgaaagca agtgccaaag ctacacttga
    60541 tgctgctcaa ggattgtacg aaagcaagtt tctaagctat ccaagaacgg attcctttta
    60601 cattacagaa aatgagcatc agtatctggt taaacatctg caaaattata aaaaattttt
    60661 aggaatagaa gaagtacaga caactgagat caaagcaaag aaacgatatg tcgatgcgaa
    60721 aaaagttcaa gaacatcatg caattattcc gacaaagacg attccgactc ctgcacgctt
    60781 tgcgaagctt tccaaattac agcaagcaat ctatttgcaa gttttgcgca ctactgtggc
    60841 tatgtttgct gcagattata tctacgaaga gacgaccgta atgacgggcg tacagcaatt
    60901 aaagctaaaa agcatcggaa agatcccatt acagcaaggt tggcaggaaa tcttacgcat
    60961 caagtcaaaa aagaaagaag ctcagactct tcctcctatc gtagaaaatg aagaagcaga
    61021 agtagaactg aaaagtatgg agaaagaaac acaacctcca aaaccctata ccgaaggaac
    61081 gttgatcaca gcaatgaaaa ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat
    61141 tctcaaagaa gtagaaggaa tagggacaga agctacacga gccaatatca ttgaaaatct
    61201 taagcagaag cattacatcg aagtcaataa aaacgaaata actgttacgc caaaaggaat
    61261 cactctttgt aaggcagtag ccaatgaacc attactgacg agcgcagaaa tgactgcgcg
    61321 ctgggaaggg tacttgagaa aaattggtaa acaagagggg acaccatctg tttttttaga
    61381 gaatatcaaa aaattcatcc tacatttatt ggcagaagtt cctaatcaaa tcgacaaggt
    61441 agacttttca gatgagatta gcgggacacg tcagataaaa gcgatggaga agaaaaacga
    61501 tcaattagga agatgtccaa aatgtaaaaa gggaatagtc atgctttatc ctaaaatcgc
    61561 tacatgcttg aatccggaat gcgacttcaa attgtggcca acagtagcta aaaagaagct
    61621 gactaaaacg aatctcagag atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg
    61681 ttttactggt aaaaaaggga aattcgatgc agttttagta ttaaaagaag atatgacaat
    61741 cggtttttct tttccatgga tcgaaaacag tggaacaaaa gcagttgatg gaaacaaaga
    61801 aacatcaaga aaaaataatg aaaacgaagt agagtaaatg aaaacaatga aatagcggaa
    61861 tatttattcc gaaaagtgag aatataaata ccaataaaac taaaaaatat cagtgaatca
    61921 agcaaaaacg ttggggtagg ctgattttat atagatagca aggagttttt ttatttgaaa
    61981 aaagtgcatt catttttaaa ttatcttatg ggagcaatca cgattgtggc tgtgtaccaa
    62041 gcatttctaa agtatcctga ttacgggacg aagttgttgt cattgacgca caatcaaaca
    62101 gcatattcac caacgttgat tttatcgact ttgactgcag gagctaaaag cgctacttat
    62161 ttttttcata aaacgacaag catgctaaag cgaaagaaac agcgaaaaca attgttggtg
    62221 gtggtagcgg ctatgatcat tatttatttt ctaccagtta ttacacaagt atttcatcaa
    62281 tattttggtt aagaatacga gagtttgtga caaaagtcat gtcacaaact tttttctttg
    62341 tcattttttc gtaagaaaaa gcaaaataaa agtatataca ttgattaaaa gtatatactt
    62401 ggatataatg tacttactat agaatgagaa tggtatagag ggtggcgaaa tatgaaaaaa
    62461 atgaaaaagt ataaaattag aaaatctggc aatagcgatg taacaactat cccaccagaa
    62521 gtaaaagaat tcatgggtgt gcagacaggt gacgcaatca gttatgtttt tcaatctgat
    62581 ggttctgtac gaatgatcaa agcacaagaa gaacctgata ttgattcatt agttgattcg
    62641 atcatgaatc aatatgaaga cgcgttaaaa gatttggttg atttatgagg tatctttcga
    62701 ctaaagaaat tatcaaaatc aacgcgaaag ttattttacg tcactcgcca ggcgaaatga
    62761 tcggtgtaaa agatgcaaat gcattagata tggccgtcaa acagccttca caagcagtat
    62821 tcaatcaaga actctatcct gaaatctatg aaaaagcggc gattttagca attaatttag
    62881 ctaaaaaaca tccttttcat aatggcaata aacgaactgc tcttgttgcc atgttgttgt
    62941 ttcttcaatt gaataattgt ccagttgctt tttctcgtca agaagctgtt gattttatca
    63001 taatgattac cactagttca ttggatttcg attcactaaa gtccaagata accagttatt
    63061 tacgacaaac cgcaataaaa tgaacgaatt aaaaacttta tttctcaatt agatcagcat
    63121 gtcattaagg aatatgctga tttttttatg tttaatcata caaaatagtt ttttcataaa
    63181 aatatggaaa atatggatta attttgaaat aattgattta aaaggaatac aaaagagtga
    63241 atagtaggaa atagtggttt agaactagtg ggctgatttt ttatatattt tagtttacat
    63301 aatatatatt atacaaagta gatttaaaaa atgataaacc tctctcgaac ggttttttat
    63361 caaaaatcgt catttttcca tcaaaatctg cttatgcaaa attataaaaa aattttgcag
    63421 tt
//
LOCUS       DACEKH010000004        62071 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEKH010000004 DACEKH010000000
VERSION     DACEKH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.004"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(28..636)
                     /locus_tag="IW662_000196"
     CDS             complement(28..636)
                     /locus_tag="IW662_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865050.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(795..2192)
                     /locus_tag="IW662_000197"
     CDS             complement(795..2192)
                     /locus_tag="IW662_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0865051.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASVLAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2224..3195)
                     /locus_tag="IW662_000198"
     CDS             complement(2224..3195)
                     /locus_tag="IW662_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0865052.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3569..3994
                     /locus_tag="IW662_000199"
     CDS             3569..3994
                     /locus_tag="IW662_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865053.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4482..5183)
                     /locus_tag="IW662_000200"
     CDS             complement(4482..5183)
                     /locus_tag="IW662_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0865054.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5199..5639)
                     /locus_tag="IW662_000201"
     CDS             complement(5199..5639)
                     /locus_tag="IW662_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0865055.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5639..6214)
                     /locus_tag="IW662_000202"
     CDS             complement(5639..6214)
                     /locus_tag="IW662_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ0865056.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7062..7541)
                     /locus_tag="IW662_000203"
                     /pseudo
     CDS             complement(7062..7541)
                     /locus_tag="IW662_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(7700..8401)
                     /locus_tag="IW662_000204"
     CDS             complement(7700..8401)
                     /locus_tag="IW662_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0865057.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(8509..9918)
                     /locus_tag="IW662_000205"
     CDS             complement(8509..9918)
                     /locus_tag="IW662_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ0865058.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(9966..11831)
                     /locus_tag="IW662_000206"
     CDS             complement(9966..11831)
                     /locus_tag="IW662_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0865059.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(11952..12803)
                     /locus_tag="IW662_000207"
     CDS             complement(11952..12803)
                     /locus_tag="IW662_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0865060.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(13113..13826)
                     /locus_tag="IW662_000208"
     CDS             complement(13113..13826)
                     /locus_tag="IW662_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0865061.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(13894..14220)
                     /locus_tag="IW662_000209"
     CDS             complement(13894..14220)
                     /locus_tag="IW662_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0865062.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(14343..14570)
                     /locus_tag="IW662_000210"
     CDS             complement(14343..14570)
                     /locus_tag="IW662_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0865063.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(14892..16145)
                     /locus_tag="IW662_000211"
     CDS             complement(14892..16145)
                     /locus_tag="IW662_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0865064.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            16206..16946
                     /locus_tag="IW662_000212"
     CDS             16206..16946
                     /locus_tag="IW662_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0865065.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(17032..17790)
                     /locus_tag="IW662_000213"
     CDS             complement(17032..17790)
                     /locus_tag="IW662_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0865066.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(17787..18815)
                     /locus_tag="IW662_000214"
                     /pseudo
     CDS             complement(17787..18815)
                     /locus_tag="IW662_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(18835..22002)
                     /locus_tag="IW662_000215"
     CDS             complement(18835..22002)
                     /locus_tag="IW662_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865067.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(22226..23110)
                     /locus_tag="IW662_000216"
     CDS             complement(22226..23110)
                     /locus_tag="IW662_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0865068.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(23205..23642)
                     /locus_tag="IW662_000217"
     CDS             complement(23205..23642)
                     /locus_tag="IW662_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865069.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(23859..24770)
                     /locus_tag="IW662_000218"
     CDS             complement(23859..24770)
                     /locus_tag="IW662_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0865070.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(25227..25655)
                     /locus_tag="IW662_000219"
     CDS             complement(25227..25655)
                     /locus_tag="IW662_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865071.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(25683..26321)
                     /locus_tag="IW662_000220"
     CDS             complement(25683..26321)
                     /locus_tag="IW662_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865072.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(26325..28361)
                     /locus_tag="IW662_000221"
     CDS             complement(26325..28361)
                     /locus_tag="IW662_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0865073.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            28487..29152
                     /locus_tag="IW662_000222"
     CDS             28487..29152
                     /locus_tag="IW662_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0865074.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(31087..34236)
                     /locus_tag="IW662_000223"
     CDS             complement(31087..34236)
                     /locus_tag="IW662_000223"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ0865075.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(34249..35448)
                     /locus_tag="IW662_000224"
     CDS             complement(34249..35448)
                     /locus_tag="IW662_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0865076.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(35438..37033)
                     /locus_tag="IW662_000225"
     CDS             complement(35438..37033)
                     /locus_tag="IW662_000225"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0865077.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(37617..38324)
                     /locus_tag="IW662_000226"
     CDS             complement(37617..38324)
                     /locus_tag="IW662_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865078.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(38389..39225)
                     /locus_tag="IW662_000227"
     CDS             complement(38389..39225)
                     /locus_tag="IW662_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0865079.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(39243..40652)
                     /locus_tag="IW662_000228"
     CDS             complement(39243..40652)
                     /locus_tag="IW662_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0865080.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(40666..42489)
                     /locus_tag="IW662_000229"
     CDS             complement(40666..42489)
                     /locus_tag="IW662_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0865081.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(43332..43658)
                     /locus_tag="IW662_000230"
     CDS             complement(43332..43658)
                     /locus_tag="IW662_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865082.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(43704..44066)
                     /locus_tag="IW662_000231"
     CDS             complement(43704..44066)
                     /locus_tag="IW662_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ0865083.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(44103..44318)
                     /locus_tag="IW662_000232"
     CDS             complement(44103..44318)
                     /locus_tag="IW662_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0865084.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            44445..44780
                     /locus_tag="IW662_000233"
     CDS             44445..44780
                     /locus_tag="IW662_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0865085.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(44856..46853)
                     /locus_tag="IW662_000234"
     CDS             complement(44856..46853)
                     /locus_tag="IW662_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865086.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(46951..47226)
                     /locus_tag="IW662_000235"
     CDS             complement(46951..47226)
                     /locus_tag="IW662_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0865087.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            47458..48192
                     /locus_tag="IW662_000236"
     CDS             47458..48192
                     /locus_tag="IW662_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0865088.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            48231..49373
                     /locus_tag="IW662_000237"
     CDS             48231..49373
                     /locus_tag="IW662_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0865089.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(49468..50859)
                     /gene="sufB"
                     /locus_tag="IW662_000238"
     CDS             complement(49468..50859)
                     /gene="sufB"
                     /locus_tag="IW662_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ0865090.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(50864..51334)
                     /locus_tag="IW662_000239"
     CDS             complement(50864..51334)
                     /locus_tag="IW662_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ0865091.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(51321..52556)
                     /locus_tag="IW662_000240"
     CDS             complement(51321..52556)
                     /locus_tag="IW662_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0865092.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(52553..53839)
                     /gene="sufD"
                     /locus_tag="IW662_000241"
     CDS             complement(52553..53839)
                     /gene="sufD"
                     /locus_tag="IW662_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ0865093.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(53852..54625)
                     /gene="sufC"
                     /locus_tag="IW662_000242"
     CDS             complement(53852..54625)
                     /gene="sufC"
                     /locus_tag="IW662_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ0865094.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(54822..55670)
                     /locus_tag="IW662_000243"
     CDS             complement(54822..55670)
                     /locus_tag="IW662_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0865095.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(55694..56380)
                     /locus_tag="IW662_000244"
     CDS             complement(55694..56380)
                     /locus_tag="IW662_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0865096.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(56373..57407)
                     /locus_tag="IW662_000245"
     CDS             complement(56373..57407)
                     /locus_tag="IW662_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0865097.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(57802..58140)
                     /locus_tag="IW662_000246"
     CDS             complement(57802..58140)
                     /locus_tag="IW662_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ0865098.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(58142..58498)
                     /locus_tag="IW662_000247"
     CDS             complement(58142..58498)
                     /locus_tag="IW662_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ0865099.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(58504..59691)
                     /locus_tag="IW662_000248"
     CDS             complement(58504..59691)
                     /locus_tag="IW662_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0865100.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(59882..60727)
                     /locus_tag="IW662_000249"
     CDS             complement(59882..60727)
                     /locus_tag="IW662_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0865101.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            60961..61926
                     /locus_tag="IW662_000250"
     CDS             60961..61926
                     /locus_tag="IW662_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ0865102.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        18071 a        12886 c        10258 g        20856 t
ORIGIN      
        1 aaacgctgat ttcacaacct ctgtttttca ttgtccatag gattgatatt gtccttgtga
       61 tgactcattc gtaataattg aggcttgatt ttcttcactt cccgttatcg tttgtccact
      121 gccagaatct gaaggacttg ttcctgtcga ctcgtttttt tgagaagaga cagttgtttt
      181 cttgacttgt tcatttgtct ttttccattc cgctaaacga tcggcgacca cttttttgtc
      241 tactttttca gaataagcgg tgatcaaacg ggctactttg tcgccatcat gagtcaaaag
      301 caaaacggtc aaatgcttgt ctttaaattg atattgtctg agagaaatcg cactaagtac
      361 gtcatcgata ttaataaatt gattttcttc ttttcctttt ggcaaataaa tgtccaattc
      421 gcctttttcg taattggcag ttattttata ctccgcccac caataagttg agaattcttt
      481 tttgaaatta tctactgatt ctttgcttgc tacgattttt tctgtttctt tctgagcaag
      541 atctgctgtc atttttcctg aaaatgctgc ggataaaata agcagtaaga aaaaaccgaa
      601 taaccaaaag agaagaaacg gtcttttttt cctcatgctt tcctccactc ctatttaatt
      661 ttgactattc tattatatac aaaaaatgaa atagaaagac aaaaaagttg aaaaagatcg
      721 taaataattg aacttctttt tcacaaaatg gtaagaatgg agaatttcac gcatttgctc
      781 tcaactattc tttcttaagc ctcaaatccg tttttatctg atacgtcttt gaaccaatga
      841 cccgattttt tgattgttct ttttccatct tgtgctaagt ctactgaaat aaagccatat
      901 cgattcttat atgcattggt ccacgaccag ttatccatac atgtccacat atgatagcct
      961 aaacaattgg caccttcttg aatggcttga tggacatatt tcagatgaga ttggacaaat
     1021 tcgatccgat aatcatcgtg gatcatgcca tcttctttga taaatcgtgc ttcgttttct
     1081 acacccatac cgttttcaga aataaagcag tcgatatttc cataatgatc tcgtacattg
     1141 atcaaaatat catagattcc ttgttcatag atctcccatc cgcgataagg attcatttta
     1201 cgaccaggca tttcataatt gtcgaaataa tcctcaggca ttggaccgtt tttagtatcg
     1261 attggtgttt cttttgcttt tacccggcgt ggctggtaat aattgatacc taaaagatct
     1321 actgtatttt ctttgataac cgtcaaatcc tctgcatgga tcacaggcat gagatcaagt
     1381 tcttttacta tctcaatcaa ttcacttgga aactcgcctt ttacagctgg atcaaggaat
     1441 gaacgattga agaacgcatc tgctatttgc gctgctttaa catcttcagg gtttgattcg
     1501 tctcttggat agcttggtgt cagattcaag atgataccaa taacaccacc ttgctccatt
     1561 tcatggtaag atttgatggc taacactgaa gccaacgctt catgatagcc aacttggatc
     1621 gcttttttca tatctgactc ttctggataa tgccatcgat ataaataacc tgcttcgact
     1681 ggaacgattg gttcattatg agtaaaccat ttttttactt tcgaaccaaa caattcaaaa
     1741 caacttttgg catatttttc ataggcttca accgtttctc ggttcaacca tccgcctttt
     1801 tcttgcagat acatcggcat atcgaaatga tacagattca caaagggttc gataccgttt
     1861 tcaatcaact catcaaaata acgatgatag aaacgaacgg cttcttcgtt gattgttcca
     1921 gattcatcag gtatcaaacg gctccattgg atagatgttc gaaatgaatt atgcccagtc
     1981 tgtttcatca actgtacatc ctcttgatac ttcgtataaa cttgcgaggt tttttcaggt
     2041 cctacttgat caaagaactt ttgcggttct tttttgtacc aataatcaaa aatgttctct
     2101 ccttttccat cgcctgaaat ccttccttct gtttgtgggc cacttgctgc agaaccccac
     2161 caaaaatttg ctggaaattg atattttttc atcaaagcac ctctttttct tttttagttc
     2221 gttttagttg attccatgta tgatattttc catcacaatc actttttcat tttcttcctc
     2281 ttcttttttg attcgtttta acagctgttc tgctgctcgt tccccgattt tatctgttgg
     2341 tggcgtgatc gtgattttcg gaaggatact cagatcgttt ttatctaaat aatcatagtg
     2401 actgaacgca atatccttcc cgattctgac accttcttga ttgaaacatt cgatggcacc
     2461 aatcgacata ttgtaattgc tgacaaaaat agcagttgcc cctttttcta ataatgcttt
     2521 tgccccgtaa tagccgcttt ttctagaata gtctcctgta aagacctcta attggatcga
     2581 tggatcattt tggaccgctt ctttggctcc tgctaaacgt tctcttgcta cgtagtctgt
     2641 ttgagtggca gcaataaatc cgattttttt atgtccttgt tcaccatgat gagcgatcac
     2701 ttttgctaca ctttctcgat cattcactaa tattgtatca atacctgctg gtccttctgg
     2761 acaattcaat gcgataactg gaatactcat ttttgtgagc atttcaaatc cagaccactc
     2821 ttcttcggat aagaagacaa tcagcccatc gatcacatgc ttcatcaggt attcaatttt
     2881 ttgttcaatt ttttctggct gatcggcaaa cccgcttaat aataagctat acccattttc
     2941 ttcagctact ctttcgatcc ctgagacaac ttccgaccca aaacggctgg aaatcccgtt
     3001 tatcaaaagt ccaatagaga agcttcgatt attcttcaac cctttagcaa tgatgttttc
     3061 tttatagtcc aacgctgaaa tcgcttctgc tatttttttc atgttctttt ccttcagttg
     3121 ctgaccattc aagtaacggg aaactgttcc cactgaaaca ccagccatct tagccacatc
     3181 acggatcgat gccatacatt ctcactcctg ccagttacat ttgatttctc tttcttgttg
     3241 ttcgattatg atgaccgcat tctttgcata gtgttgcttg tcatcaatat ccatataact
     3301 aaagtcttca gtagagcaac ttcctctcac tattaattat acttaaacaa gtatttgaac
     3361 cggttcattt ttaatatagc atttttttaa aagattgcaa cactttttta aaacgctttc
     3421 tgaaagtggc ggattttttg cctttttaaa attttattct aaaatttact ctatttgtgg
     3481 gtgttttgtg cgtttttctc ttccatccgt ttgaagtttt acattccaat ggtataattt
     3541 ctttatccta tcaaatgggg gaagtatttt gaagaaatta atcgtactat ttattttgtc
     3601 tgcgggactt ttgacattga ctggctgtga tttgtttggc ggctctgacc aatcctcgga
     3661 cgaaacaacc gattcttctg tcttaaaagc ggaaaaagca gataccacca acagtgtata
     3721 tacgctaaaa gtacgtaaag atatggattt atccgcggga cagttgacat ttacagataa
     3781 aaaaatggaa tggacacgca cgtatcagac acaaggaact gattcttctg aatcatctga
     3841 agaaaaaaca gtgctgacag atatcaagat cactaccaaa gatgacgaat atattatttc
     3901 aggaaaagaa aataaaaaag aaacaacagt catatttaca aaaatcggta attaccgttt
     3961 gaaggatgag gatggcaaca tctacagtct atagtgccgt ctgttggatc ttcttttgtt
     4021 tctccccttc ttttctgcta tagtaaagaa gcaagatgat tgtaaaggag acgaaatgga
     4081 tgatcgatag tcctgattat tgtacatgta ataacaacaa aaatatccat acagaattta
     4141 atgaccaagg aagcttgagc atatgcgaca actgtcacaa actagtcaaa gattcattta
     4201 aaacatttga atcttatcct gttcaaaatt atgtaaaaag tagaagaaga taaacaaatc
     4261 caagcaaaac tcactatggc tgatttttat gaacttcaaa ctcagcttct tcctttgatc
     4321 aagcttcatc agtaaaacaa acagtggatc atttcagttt acaaatcaaa gagtaaagat
     4381 ttatttattt caaatatttg aatataaaat aaccaaacga tagaaaggct tattctacat
     4441 cacgtagtaa tttttctttc ccgtcgtttg gttttttatt tttagcctaa ttcttgacct
     4501 tttgcatgct ggttatccgt aaaatatttt ccagcaggct ttccttccaa ttcagctaat
     4561 ttttcttgga tctgacggat cacctttttt gcatcttctg gattttctac aaaatcgaat
     4621 ttatccccat ccacttgtat tttaggacat acatcaaaat cttcatacca ttgattatag
     4681 cgggtgttca acgtttcata ataggtatat aacgtctcat cgctttctag ctgttcatag
     4741 tcacgcccac gttttttgat acgttctagc atcgtgtcca aagaaacttt gatatggacc
     4801 atcaaatcag gccgtttttt tggagcaaca tcatctaact cgtttagcat cgtatctaat
     4861 aagctatcgt actgctgtac ttcgatatcc gttacccgac ctaaatcagc gtttagatgg
     4921 aacaacaggc tatcttcata gatggatcgg tctagcacat tgtcatcgtt cactaaagca
     4981 tttttcattg ctaaaaatcg cttgttcaaa aagaagattt gcaataaaaa agcgtactgc
     5041 tcaggatttg aataaaagag cggcaatact tcgttatctt cgacattttc ataaaatggt
     5101 ttagaatcaa tctcttttgc aagcatatcc gtcaaactgc tcttacctgc accaatcgtt
     5161 cctgaaatca aaatcactga catacaaccc ttcctcttct ataagtactc gcctacgtaa
     5221 gtttcaacag gcaatgtttc aaaatcatat tcacgtacag cttggtctga ttttaaatac
     5281 gtatggagac tttgtgtaat catcaagttc gtaggtaccg tctcttcttg tacaatgatc
     5341 atcggttttt tcaacatagt tgccacacct aattcgtaag ccgttcctgg atcgattgtt
     5401 tgatgctcaa agtcaaggat agcaacaaca aattctgcat ttgtcagttc ttccatatct
     5461 ttttcgtata cttcctgcgc ccatccagcg ctgaatagtt cataattgct ttcttggtga
     5521 tgacgtggag agtagaaact tgtgactgtt ttgttttcct ccaacgcttt ttcgatacga
     5581 cttactcgat cgatttgctc ctctgaaaaa aatggtcctg ctaaatatat ttgactcatt
     5641 attttgcctc cttcaataat aactgatttt taatagatac tgctcgtaat tcattgacca
     5701 gttccaatgg attgttggaa aagattactt gatccaacca ttttttatcc ataaaatcct
     5761 cttttgcaaa agtttctaat aattgttgca agccgctgta ataaccgcca atattcaata
     5821 ttccgattgg cttgtcgggc gtcagaccga ttgctatcca agaaagaagt tgtgagaatt
     5881 cctccaatgt acctagacct ccagggaaaa cgaggacagc atctcccttt tcaaataata
     5941 atacctttct ttcatcgatc gtttctgtag gaataaatgt agtaacatct tttccaggca
     6001 attcagtttc aaaaagccca ttcggataga tacctgtgac ttttccgtta ttttccagta
     6061 ctgttttact aatcactccc ataatcccgg agtatgaacc accgtaaacc agttcgattt
     6121 cttcttgtgc catgtatttt cctaacgttt gcgcaataaa tttgtaactt tctttattac
     6181 caaaacggga accgcaaaaa acagtaagtt tcataaaaaa acactcccta actcaaaagt
     6241 ctatcttcat tctacacgtg tttttttaaa ataaaagact agattttgta gttttttagt
     6301 ggtatatagg atagacgcca ctatatatag tatttagaaa aaaatcattt ttcagccatt
     6361 ttatagataa aacaaaaata aataacgtgt tttcatcgat atgaaatgac ttacttatag
     6421 aagatactgg gatttcgtcc tatatgccaa taacgtgtat tattttaaac aacaaatact
     6481 tgtttctttt attaacgaaa aaatcatttt gttctcgtgt tatgtttcac tgataaatga
     6541 tgtttctcta aataaaaaag ccgacgaact aaacgatcaa aatttgatca tttttgttca
     6601 tcagcttctc ctaaaaatct ttttgttaac gataaatagc tggatttgta aaaatatcac
     6661 ttgaatcgtc actgctggaa gaaaattcag tgataaccgc acctgttgcg cctgcctgga
     6721 actagtgttt gatattgggc tcgaacgtgt actgatctct agcatgaaga ataatttctt
     6781 tttttactgt ataataactt tctttccttt ttggaatagt acaggaaagt ttttttgttt
     6841 cctttccttc gacataaggt ataccagacc ctttcgacaa cgaaaggttc tcatttttcc
     6901 ttcatcgtca tttcaaggag ggtgccgatg ctctggacag tttcgatcgc tcctaaacca
     6961 aaatctgtat attcgattct ttctaactct gattctttca taccattcct gtctcaaggt
     7021 acatctattg tacttttttc ttgtaatcca ttctgcctct cctaactaat tgtcaaatca
     7081 tcccaagata tgtcttcttc ctctttttct tcttggatga tttctgcatt cggatctact
     7141 ggttttttca ataataaaat ggttattcct gtgacaaaag aaccagctaa caatgcacca
     7201 atgaatgccc atggtctggt catcgtagcg actgcaaaca ttcccgatgc cggaatacgg
     7261 ctatctgcgc cccaaaacat gcttaatccg ccacctactg tcccaccgac tcccgttgct
     7321 atgacattgc gtgccaagtc gttcaatacg atagggaaac atccctctgt gatctgacag
     7381 atccccatag gaaaagcggt cttcaatgta tcgatttctt gacgattgag tatattttta
     7441 cgaatgattt tccctaagaa ataagcgatc gtcaagccga atggggttac catcgaagct
     7501 aaaatcaata cagtgattgg ttcatagatt ccttctgaaa atcgtgccac acccccttat
     7561 ccagaataaa tggtaatcca gaaacagccg tgtatctgcg gtaataagcc taaccaaacg
     7621 aaaaatatga gaagctattt ttgtttggct gttcttatta cttgatgcag ggcagctttt
     7681 tcacaacctc tttttttatt tatgtttcat aatcaatatg gtaaggatgc agctccgtat
     7741 ggtagaccgc ctccgaatat tcaatcattt ccccaatttg cgtagacgat ttccgtatcc
     7801 gtttgatgcc gatctgtgcg tctgttttca gaattccttg ttcttcttct gttaaaaata
     7861 ctgccgagaa atcatcagaa aatgagtcaa tagtatatcc ttttttcgtc agcaaacgat
     7921 acaaagattc tttgccaaat tcttctctgc tgaattgcat catttcttgt ggaagataat
     7981 gaacaaaata aatataaggc tgattgttca aataatataa gcgcttaaaa caaacagctt
     8041 gctcaccgaa aaaggaaaac aaaggatctt ccttctttat atggaccaat tgcaaatcca
     8101 gcgtttcttt ccggacttca tatcgggagt tctccaagat ttgcgtaaag cttgccgctt
     8161 tggataatcg attgtaagga cgattgctta gaaccgtcgt cccacgacca cttttctttg
     8221 cgacatattc atctgatgcc agcagttcga ttgcctttcg gacagtgatt ttgctgactt
     8281 gaaacagttc ttctaattca ctttctgtcg gcaacatcga accgactgga tattttcctg
     8341 agaaaatatc ttctttaatt ttatctgcga cttccaagta tagtacacgt gtccttttca
     8401 taacgtcacc ctttgcttta ctattactaa aagaatagca cataaaaaat aaaaaaagaa
     8461 cacaaatcac atgtgagaag tgtgttcttt ttcattcagc tgctttaatt acaagtttac
     8521 gccatttgag gcaattactt ctttatacca atcaaaagat tttttctttg atcgattcag
     8581 tgtcccattt ccttcatcgt ctagatccac atagataaag ccgtaacgtt tactcatttc
     8641 tcctgttgat gcactgacaa gatcgataca tccccaaggc gtatagccca tcaaatctac
     8701 accgtctgct actgcattag ccatcgcttc gatatgacga cgtaaataat cgatccgata
     8761 atcatcaata atcgtgccat cctcttccat atgatcaatg gctccaagac cgttttcaac
     8821 aatgaataat ggtttctggt aacgattgta taattcattc aatgcgattc tcagcccttc
     8881 tggatcgatc tgccagcccc attcacttgc ctcaagaaac ggatttttta ctcctcccag
     8941 caagttccct tctgaagttg ctgcttgtgg atcagtctca ttgattgccg tagacatata
     9001 ataactaaat ccgatgtagt ccactggata ttcttttagc aactggaagt ctttttccgt
     9061 ttgctctaat ttttccggat cgactccata ttttttatgg gtacgcgctg tgtatgcagg
     9121 gtattctcca cgtacttgga catccgtgca gaaaaaattg aattcttggt tttggatcat
     9181 tgttgctgcc tgattgactg gattggcatc gatgctgtaa gttgttgcat agataatcat
     9241 gcagccgatt tggatatccg aacgcaattc gtggccgatt tttacggctt tgctacttgc
     9301 aacgaattga ttatgccatg cttggaaaat attttggtac tctcctgctc cattgctttt
     9361 tactaatcct tgacttaaag ctggaaagtg aaaggccgaa ttgatttcat tgaaagtcat
     9421 ccaatatttg actttactgc tgtatctctc taatactgtc tgagcaaatc gttcatagaa
     9481 gtcgatcagt tttcgatttt tccagccacc gtattctttt gccaaatgaa gaggcatttc
     9541 gtaatgagaa atcgtgatca caggttcaat atcatatttt aagcactcat caatcagctg
     9601 atcataaaat tctaatcccg cttcgttagg agtaccttcg tctccatttg gaaaaattcg
     9661 tgtccaggca atcgaaaaac gataacattt gaatcccatt tccgcaaaca aggcaagatc
     9721 ttctttgaaa cgatcgtaat gatcgattcc tcgatgattc gggtaaatat atttttcttg
     9781 atcaatcgtc cagtcaaatt cttcactgcc gataatcgca aagcgctgct tacctccagg
     9841 catagcatct gcaacggata gtccttttcc gtctcgatca taagcccctt ctaattgatt
     9901 tggcagcagt tgctccaccc cataaaaatt tttctgaaaa acgtgattgc atcctgattt
     9961 cctccttatt ttacaattgc cagaacatct tcgtttgcaa taatctcttt ttggtctaat
    10021 tcaagtatat cggcaaatgc atcagtattc gtaatgacaa ctggagtgat tacgctatag
    10081 ccagctgctc taattgcttc gatgtcaaaa cgaatcaata aatctcctgc tttgacgaga
    10141 tccccttgtt tgactaagat ctcaaagcca ttcccattta attctactgt atccatcccg
    10201 atatgaatca aaatctccgc tccgtcagtc gttgtgatcc ctacagcatg tccagttgga
    10261 aaaatagtcg tgatctctcc gtctgcaggt gcaactagtt ctcctacttc tggatcaata
    10321 gcgatacctt ttcccatcac gccagaagaa aaaacttggt ctggtacttc actcaatggc
    10381 agtactttac ccgttaaagg actttttaag gtatgccgtg ctgaagtaat cggttctcct
    10441 gcattcgcat gtttgttttc taattgattg tcttgtgttt gttcgtcaaa tcctaaaatc
    10501 aatgtaccta cgaatgcaag aacaaaagca attcctgtag ctattactgc tactgtcaca
    10561 tttgactcta caccatccac cgtattaatg aaagtcggaa tcgtcaataa gctgaccaaa
    10621 ctgctggaga aagcttttac tccggaaaat ccgataatag ctccaccaat acctccagaa
    10681 atacaagcgg caatgaatgg tttcttcaaa ggtaatgtca caccatacac agttggttca
    10741 gtgattccaa ataacgaagt cactgtagaa gaaatcccta atgctcgaag tttcgtatct
    10801 tttgttcgca atgctacagc taaagcagcc ccaccttgtg ccaagatggc tggaagaagc
    10861 attggcatca atacatcaaa cccatattgt tcgatattca aaaacatgat cggtacgaat
    10921 ccccgatgca tgccgaacat cacgaatact tgccataaac ttcccatgat tagacctgct
    10981 acgattggac taaatccata aatcgagttg aagagaccgc ctaaagcatt accggcaacc
    11041 gtcccgattg gtcctaaagc aagaaatgtt aatggaacca tcactactaa aacgacaagc
    11101 ggaactaaaa tcatttgcag aaattgcgga atcacttttt ttacgaatgg ctctaattta
    11161 ctttgaaccc atacggctag aatgattggt atgacagaag aagtataacc agatggtgaa
    11221 agaatcactg ggatcccaaa gaaactgata gcttctcccg caccagcaac tgctgtgata
    11281 gctggataca caagtgccat agcaattgct actgctaaaa actggttggc tttgaacttt
    11341 ttagctgctg taaaagcaag catgacaggt aagaaggtaa agacaccatc tgcttcagca
    11401 aacaatattt gataagtacc gctatcagct gtcagccagc ccatcaccgt tgccaatgat
    11461 aagaatcctt tcaatacccc ggcggctgcc atcgccccta gaaaaggtgt aaatatacta
    11521 gagataatat cgatcagacg attcaaaata tttttggact cttcttttcc atcctccgaa
    11581 actgcaagac cttcctcatc cacgatcgat tgataaacat ctgctacatt actgccaatc
    11641 actacttgat actgaccgcc actttgtacg acttggatca cgtccgggtc ttcttttaac
    11701 ttttgcgtat ctgctaaaga ttcatctttt aatctgaatc gcaaccgcgt agcacaatgt
    11761 actaaacttt tgacattctt ttgtcctcct accgcttccc atacacgacg accaactgct
    11821 tgattatcca ttgttttttc cttctttctt ttttatttaa aaaataaaaa cccagattga
    11881 aaaaaacagt cacaaacatt cgtgaaccta ttttttcaat ccaggttttg cctgccatac
    11941 agtaacaatc cttatgattg gatgcttttt ttctcactta ctactctttg aatatgaatc
    12001 gttaaataaa tctgctctgc gatccccatt tcctgttttt gcgtttgtcg aagatactca
    12061 ttgatccgtt cgacacaatg aaacgctttt ggatactttc cttgaactaa tgcgtagaga
    12121 aattcatctt gctcgtcaga tccttcccct ttgagataac gctgggcaaa aaactgcaga
    12181 tgagtaatga tacgctgata attcaaagat tcttcatcga acacgatgcc gaagaattta
    12241 ctgatgatac tcagtatatc tcttaccatt gtggtagcgg tcatcgtcac ttgcatgtct
    12301 tcgtttcctt gttggctatt cacaaaatga aaggcaataa aaccggcttc atcctcagaa
    12361 agttccacac ccgtcttttg tttcacgaac aaaagagcct ctttcgctgc ttgatattct
    12421 ttcggataaa atttttttgt ttcaaataat aatggattag gcagttggat attttctttt
    12481 gctcgtgtga tcgcataatg aagatgatcg gtcaatgtca gataaatatt aggcgaaaat
    12541 tcaactttta gtttatcttc agccagttcg actacttgtt tgacccattc gatctcctct
    12601 gccggaatat cagccatcag ctgctggaat tggtttttag caaaagaatc tcttaaaaca
    12661 aattcttttt ggaccgcttc tggcgaaatc tcgtccccaa tctttttttg aaaagctagt
    12721 ccttttccca tacaaacgac ttcttccccg aatccatttt tcgaaatgac gacattgtta
    12781 ttcaaaattt tatcaatttt cattttgctt cgccctctca agaaaaataa aaaaacctag
    12841 aattcgccta ctgtttctta gctgaattct ggttttgcct gccgtacagt aacaatccat
    12901 ttcttattca aaatcatcat accatcgaat ttttatttat gcaagccttt tcatataaaa
    12961 aataaaaaaa ctggaacaca agtctatcta gaaaacaacc aaatcagaga tgcacctctt
    13021 tgcgtttctc tttttttcag caaattggtt tttccttatc gattcatatc ctttggctgt
    13081 ttttaatctg taacgactta tgtcccagta tcctaaaatg tttttgtctc tagattgaaa
    13141 gcagatctca tatcatctgt cgcatctttt atcgatgtaa cactttccag ttctccttgt
    13201 acaactagtc gcgtgacggc gtccatgtcg ccacaagtaa ttagtgtgac aatttctttt
    13261 ccctcgactt cattcagtaa ttgggtatct gtaggagcaa ctttttcttt aaaaaatgtt
    13321 ttgtatgtat acacattttc taaatctgtc aaatagatct tggcacccat ctctaaattt
    13381 tctagcggtg taaacagcaa ttcagggttc gttgcacgat ggcttgctaa ggcataattt
    13441 ccttttccca tttcttgatc tggagataat gttcctgctc cgtaaagtag tgacgtatta
    13501 tccaagcctt tgaaaaccgg taaattgatt gaaactgaag ggactgcgat actagcaatc
    13561 actggtagat ctgttcctct catttgcgac ctcatgaccc cttctgtcgt cattggttct
    13621 accgcatcaa agtcaaaact gacatcttta tcgttattct ttttcagatc ttcttttgtt
    13681 acattcgcta tagcatattt atctcctgtc tctctgacaa aataatcctt gatctgttca
    13741 ttgaaaatca aagctagtcc tactaataac aagaggaaca agaggatatt gactagccat
    13801 ctttttgctt ttcctttttt tttcattttc tctccctcca gactcatttt actgtttttc
    13861 ttttgctctt tccaacagcc gaatgacaca tttctacaac tctgtgtatt tttttcgtac
    13921 acctgcgctt ttttctacag gatatttcaa attatttttg atcaattttt cttcaatgat
    13981 ctcatctaga tccaggtgaa ggttgtctgc catcatgtat gaatagatca atacatctgc
    14041 cagttcttct ttgattcgtt ctaattgtgt ttcttttact tcttccggcg ttttccattg
    14101 aaaaagttct aacaattcag atgcttccaa agaaatagag atagccaaat ctttttcgtt
    14161 atgaaattgt cgccaattac gttcatctct aaactggttg actttttcca tactattcat
    14221 ttgcttgcct tcttccaatc acttcttact tgattaatca taccattttt ttacataaaa
    14281 aatagatgat gcttttaaaa aaactcctgt tttcttttat ttaaaataaa tgtcatagaa
    14341 aatcaataat ttaggtagcc accatgtgta tgcagtagcg tgtcttcttc ccttgaggct
    14401 ttttgttttt cgatacgttc ttcttcccaa acagacaacg ctactagaat ctctcttaat
    14461 gattttcctt cttcggtgag cgagtactct actttaggcg ggacttggtt ataggctttt
    14521 cgatggatac tttcatcttg ttctaactct cttaactgct gggtcagcat tttttttgtg
    14581 atcgtaggga tttgcttgct tctccacgcc taaaataagc gatattgctc ttccctttat
    14641 ctggtgtaag caaagcagtt agcattttga cgatcgtggg ttccgctttt cacttcttat
    14701 ctacttagta tcatgaagta tactttcgta ctaatactaa gtagcggaag tgtcaactac
    14761 tttgtttaac tacatagcaa aagaaacaga agggtgacaa cggtcatcac gcttctgttt
    14821 cttttgaata tgctgtgttg ttgtctctat ctccgtgtag actttcctca aacttatcct
    14881 ttgacagtaa gtcagtaggc tgatttgctc tttctagatg ccagatacat ttctacaaaa
    14941 aaatagtgat tctgttttga aaattcgatt tttagttctg cattttcgta aacatccaac
    15001 atttttttcg cactgtatag acctaacccg ccattttttt tagaggtttt tcctcttttg
    15061 accatctccg atagcgtcaa attcgttgga tttgccttgt tcgctatact aaaacgtgat
    15121 ccctcttttt cttccagcag attgatcgta atcggactct gatcggctac acgatgatcg
    15181 aatgcgttag aaataaaaat agataaacag cgaataaaaa caaccagatc aatcgggatt
    15241 gttttcactt cgacaggaat aactacttgg atcgggattt tctccgattc tgctttttct
    15301 agaaatttta aaaagacacc cctgacagct ggggatacaa tatttgttag ctgttgtatt
    15361 tgcggttttt caaaaatagc tgcagaatga tcttctaata tttctaaaaa ttccaacgtt
    15421 tcttttttat tttcctgaag agtaattttt aatgaaaaca gtagactttt ataatcatgt
    15481 ttgaattcct ctagatgatg gtaatattct tcatttattt gatacagttg aagcgattcc
    15541 tgatgttgtc tttcttttac ttggaaactg atcaacataa tggaaaaaaa caaagaaaaa
    15601 aaactgatga ctatcgacaa aagagataga agcagataat tagatgagcg gaagtgaaca
    15661 cttcccaata ccaagtaaat cacgccccag cttaataaaa tacaaatacc tccaaaatag
    15721 tgttcagttg gataagtaaa aagcaagttg agaaatctcg tctgtttgtc tatacttttt
    15781 actaatagat agaaaataaa tgcgataagt agtgcaaaaa aacttgctgt tccaagatgt
    15841 ctctttccta aataatccat cagatcaaaa accagaaaaa agctgagtcc agtgatagca
    15901 aaaaaaagat tactgatcaa acagctgata aacaggtttt tcgtagtaaa ataaaaaaga
    15961 aacatcaata ataagccaac taggtaagga aagctcactt tagggatagg aagtaaaagt
    16021 gtcaccacca tcaaagctat acaaccaaag ctgtttttta ctagcagttg tttcgaaaag
    16081 aataaataga tacctaaatc taaaatcaaa gtaaaggtca aataaagtgt cactgttgcg
    16141 accatcttat tcctccattg ttttgtttca aaatgtacac atattttttt aataggagcc
    16201 tgcatatgaa aaagtatttt atcatagaag ataaccagtt tcacagcgaa tggatcatca
    16261 gagaattggt caatcatcat atcgagaata tcgtgcagct cacttctatc tctcaaatca
    16321 atcagttgat ccagttgggc actattgata atcacgatgt tttctttatc gatatcgact
    16381 tgaagcaatc actcacaggt attgatgtcg ccgaacaaat acgtaaaaaa aatgcacata
    16441 gtaatattgt ttttttcacc gcttatacga attttgcagt agagatcatc aatcggaaaa
    16501 tccttccgat tggatatatc agaaaagaca ggaatttatc tgaacaaatt cgtcaagtgc
    16561 tagaacagat caattggaaa gaacagcaga tcgtaaatgt cgataaaatc tttattgatc
    16621 aaaaaggtgg aagtctcgtc ctgtatcctc gggaaatttt atatattgaa agtatcaaag
    16681 gtttgaaaaa ccaagttatg gtcaaaacaa ctacagaaga taaattagtg aatacttctt
    16741 taaaacaatt gaagacagag ttagaaaagt atgcttactt tttactttta aaatcttact
    16801 gtatcaactt atcgcaattg cagaaaattg attctgctca ttatgtgtta gaatttttca
    16861 acggtgactc cttactcgtt ggtcgaaaga tctttgaaaa gacaaaggaa cgtttccatg
    16921 acttccaaaa aactgcttcg agttgatgaa taagagctga gacgtaagca tagcagattt
    16981 cagggagtgg gccttgaatc ggctccactc cctgaaatcg ttgatatgat tttacgcttc
    17041 ctcttctgta ggagcatctg ttaacgacca tgtgaatgtt gaaacgtagt tgtctacagc
    17101 ataattggat acaggaacaa ataattccac tgattgtctc ccttgttcca cattctctcc
    17161 taatgaataa atccatgtgc caatcccttc atttgctcct gcagttacta gatctactgc
    17221 ctgatctgct gggatttcga ttccttcagc gccagttcca gaaacggtag atggcattcc
    17281 actgctatta gttgcgctat ttcgaatcac ggcgtttttg aatttcacct ttgaaccagt
    17341 tagtttattc gtatcagatg ttgtaccaat aaattctgtt cgtttgactg ataatttcca
    17401 accggcataa tttccgcttt tatccgttac ttgtacgaaa ttctgtactt gtgtttgtgc
    17461 tccagtactg tccgttccgc tttgtaatgc agcatatact gtttttgctg tacctaactt
    17521 gattgttcca aaatcaagat ctggagcttg gtcaatcgtc agtaatccgg atgttccctg
    17581 atttggttcg tcaggtgtaa aaggagtatc aggatcattt ggatcaacag gaggaactgg
    17641 aacaacgtct tcaggatttg cagcatctgg tttggtcaca gaaatcgttc cttctgtagt
    17701 ggctgagctc ttattctcag caaaaacact ttgtgtacat aataatagac ttccagctag
    17761 aacggtactt aatgcaattg tttttttcat ttctttttcc ttctttcttt taaaataaat
    17821 aatccagcaa ataagccaat aagaatgact ccaacaagta tcagccatcg ccagtcaaat
    17881 gtttctggct gattttttaa atcaatatcc ttttcattca attggtccgc tgcatcatca
    17941 gttatttcaa aatcggtatg gagattccag cgtttttctt tccaagcacc cttcacaacc
    18001 gcttgataat gtcccggagc taattttttt ccttctaatg ggacaagtaa atcaaaattt
    18061 gtattgggtg ctacatctat tttttctttt tttattgaaa ggtaaggctc ttttgtttct
    18121 tttttgtaga tttcaaccga tgtagtcaag ccgaacatat aagaagcatt tggattttga
    18181 agattcgcct taatcacatt tcttccatcg ctttgatccg cataagcttt ttttaatttc
    18241 aaatctgggg aagactcttt ttcgctattt caaatcaaga ttgctttcac ttgagaaaac
    18301 tgattgacga caccaactgt acttttgtta gaaacgttct cttttttttc ttgaaacgtg
    18361 atccctcctg cgaccacacc agccagctct ttttctggcg ctttcagtga aagtgtctca
    18421 gtgaccgcac tttgaggagg aacagttatt tctcccactg tttttgctaa gtttttcata
    18481 tcataactta atgtttcatc aaatttactt tttgattctc catattcgat cacaccgttt
    18541 gaattcgtcg tagcggagtg gatcaatgtc tcaactgtta ctgatttatc tgttcggtta
    18601 aagaccgtgt aggggatttc ctgtgttttc ccaggctcca tccttacatt tatatatgtt
    18661 ttgttcttat caatctgatt agccggtaat tcaaaatcta ccgcaaaatt gaattcggag
    18721 gcatggacag gtgtctgcca aaggatgaat ccaaggagaa ggatcataag taaagagcag
    18781 ttgtttcgat gacttttatt ccatattcga ttcatcaatc atcctccttg atctttattc
    18841 tgtcggcgca tctgctaatg tccatgttat ctctgctgag taattaccag ctatctgatt
    18901 ttctttggtt aagacaagtt taaacgcccg accgttattt aaatattctg aataatctgc
    18961 ttgcacattc gttgaatttt gtcctctgac aatttccagt tcttcttcat tcactaattg
    19021 atactcctct cctttcttga aatataggac atcctgcata gaaaatcctg catcatttgt
    19081 gaaaggctga gataatttta tcgttaagat ccacggattt ttcgtttctc gctgatcata
    19141 aatccataat ttttcctgta tatccatttg gccaaatgct tcttgtgtca aaccagttgc
    19201 tttgatctga ccaaaatcga gtttggaagg ggcaagaaat gagagaccac cttctactac
    19261 gatcttcgta gcaggtgcaa aaggcggggc atcatgataa acaactggcg tatcacttgg
    19321 attgtgattc ccaatttcat cgttggtact tggcttgttc tgcacaccct tagcagtagc
    19381 ttctcctgct tggtcgttta atacaatttc taatacatct cctgcaaaaa gttgattttc
    19441 tgggatagaa agaacaaatt cccctgtatc atcaatagtt gtttctgaca aaacagcttg
    19501 attttttttc aatgaaacca aggctcctgc ttcacctgta cctgtgatcg tgcgagaagc
    19561 tgcattgatc aatgtggtac ttaaattagc aggttcagga ggaatcacat cgactgtcgt
    19621 tttggcagag tccagttctg tcacttcttg gccacctctt acttttttag cagcaacaac
    19681 ttgcacttga tcctccgttt ttaatctctc atcattgggt acttgaactt cgaacaagcc
    19741 gccttgttcg ccttctcccc atgggttata tcctgcccct tcattttcat ctgtttgtcc
    19801 ttgcgtgaga gcagtaattt ctacagatga tttcgtcgta tctgcgtaag taatacgtaa
    19861 agtcacttct acttcgcctg tccaagcatc tcttggcttt gcctcgacac cttcgggaac
    19921 aacgacatgc ccatagattt ttttatctgc attcgttggc actcttaagg attcaatcat
    19981 cgggagctga ttattggcac tgattcgatt ataatttttc attccggcac ctgtctgagc
    20041 aaattcattt gttaaacctg cactagggct tgatacgaaa ttgcctaagt cggtcccatt
    20101 gatcgataag tctgtcatat cagaaaaata atccgggtct ttttcaaagt tatcgaatgt
    20161 tgcgcctgtt tttcgccata aagcgatttc tgaatgggcg atctttaaag aagaatctcc
    20221 atttccttgg aatacccagc cgcctatgcc attggtctcg cttccaatcc gtttgttttg
    20281 gaaatcaaaa taggtaggag attctaagtc cacattcaac acatccccac tgatgatgcc
    20341 ggtcttatct tcccctgttt tcccgaccat gactaaataa gttttttcac ctgccgtaaa
    20401 atttaaatca atatctccag ttgtatctac agttgctccg gaatctgcgc gtatatcgat
    20461 atttgatttt tctccagtga ctgtaaaatc agctgaacct ggactttcta gaaaaccaga
    20521 agcgtcataa aattggatcg cttgatttcc ttgattgagt ctgttcacgt aagatttgcc
    20581 atcacctgta tttcttaccc tcaatgagcc tccattggtt actgtcagct tttgcgtacc
    20641 attctcaaaa cgaactgcgc tggaacgccc actctgctta tcgatgtcga taactgatcc
    20701 ggctccttct acgatcaaag aaaggccttt tcctacaaaa cgcatcccat tgttcccggg
    20761 ttgatcgtca tctccagcca ttttgaccga gatttgtcca ccacttgata catggatttg
    20821 actagaatct ccttgcaagc gaaatccgcc cgtagttgct gtattgatgg tgattttccc
    20881 accattcgat acagtgactt tcgatcctga acctctcatt tggatgattc cgcgattgtt
    20941 gtcattggta atattgatct ttgtatttac cgaaaattcg cttcccgaac cactgacttc
    21001 aaacatgaca ttaccaggta gcgtatcgac tgcctctcct tcatcgctaa cgccatctgc
    21061 ccgatcataa tcaagagaga cctttgaacc gcttaagata tggattcctg gcgctgtttt
    21121 tccactacca gtctcatctc ctgaatctag aaaaccgtag aatttcccta gatacggtcc
    21181 ataaagttga ctaccattga tcacattcaa ttgatagctt ctacaaaaat aggctttacc
    21241 cggagcggca ttcagtcctt ccttcactgg ttggacatcc aacacaccgg aaacaccatc
    21301 taatgtgaca gttgcacgag gagcgtaaat cagtcctaat ttggacccat catacaaatc
    21361 aagtgtcccg gtaaatttta tttctcccgt aaaatgatat ctgcttcccc aaaaatcggt
    21421 catataagca atgataatgg catttccatc gatatttggt gtcaatccac ctggatgtgc
    21481 agctgttcct ttaaaattct ggaactcgat cgactgaggt atctcattga tataaatatg
    21541 gtgtgtgccg aaattgtgag cgaatccacc aaaatcaata acgatatctc gtttgatctc
    21601 taattccctg tctaaagtca aaggctgaat cagtcggatc ttagttactg attcgtctgc
    21661 gagaagctgc gccagttctt ctgttgtggc agcttcgctg tacccatccg ctgaaacagc
    21721 tcttgccagt gccgcttcac tgctatcaga cgtttgttca gctacttctg aggtctcact
    21781 tgtcttattt tcttcttgcc ttttattagt agaagcctca ctttgagatt ctgtagtttc
    21841 agaagtaatc gattcttctg aaaaagtagt cttgtcaaaa ctttgattct ctgattgcgt
    21901 tgattcgttt gttttctcta catcggcaaa aacggatgtc accatcaaga gagggaagac
    21961 aaacaaaatc gacgcaatca gcttttttac acattgattc attctccacc atcctctctg
    22021 acgttttgac aattttattt tatcgtttta atctatttcc aattgtactt tcatatttaa
    22081 aatatgtatt tttatggaaa aatttttcta ttaggtgtaa atttcttttt tgtacaaaaa
    22141 aaaggctgtg acaaaagtct tgtcacagtc ccttatcccg aacaagcggt gttctcccgc
    22201 caactcctcg gtcacaacct ccattttaac aataaacctg taaatgtgag ggcaatactt
    22261 tgattgtcac aggtaattta tccccttcat ctccatcaac attcgtcgtc aattcttcct
    22321 catcagctaa tgacacaact aattgctcac aagtaaggta ggctagattt ttcgtagatt
    22381 catctactcc ttttaatagg tctggcaatg ttttaagcgt atcgatcaaa gaagaatctt
    22441 ttaaataaac caaatggagc ttaccatcat ccacttttgc atttggaagt aatgtttcaa
    22501 aaccgccgac tgagttagtt agtcctatca gcaaggtact gctctcaatt tcttccttct
    22561 ttccatcaac ttctacgtga aacgagtagg attgtgtgga agctaactgt ttgattcctg
    22621 acatgaaata agctagtttt ccaaatttcg tcttcttctc cgcatctaca tcattgattg
    22681 attcagggat agagccaatt gcgacgacat tcataaaata gtcgtcattt atttttccaa
    22741 tatccaatgg cttgactgat tcaatcgaaa aatgattgat cgcttcctca ggttctagcg
    22801 gtatccctaa tgctcgagcc aagtcattca ccgtacccaa tgggaaaaat ccgaaatttg
    22861 gccgatgttc ttgttcagcg attccactga ttccttcgtt gactgttcca tctccgccca
    22921 tgacaaacac actatgatac ccttccgtag ccgcttcgcg agtaaagttt tttgcatccc
    22981 ctgctttttt tgtatgcaag acgaccactt catcaaacac cgattccaat tttgctatcg
    23041 ctaattgctc aaattccttt gcttgttcac cgcctgaact ggggtttacc actaataaaa
    23101 cttttttcat cactttcttt cccttctgat tgatttcttt aatataacga tagcataaag
    23161 aaaaaaatta ggctgtgatg aagtactgtt catgaaacat tcatttattt tttgacaaga
    23221 taaaacatcc atactatgaa actgaggaaa attaaaacaa tgatatagga aataaaagaa
    23281 aatgataggc attgtatacc taataaagta aaaatcttag gtggcagata aaaacacaaa
    23341 aatagcaaga gaatgtaata gcgtttcttc cactttttgt tatcagacct tatagcgatc
    23401 agaggaatac caaatgcata actgaaaaac tcttgatgta ctgttaatat gtcacggcct
    23461 tgaataataa atgcgacaat ccccgctata attagtgtgc ctaacaccaa aacggaaaat
    23521 tgatgaatat attttatata caaatgatcc attgtacttc ctgccttttt atttgtgaga
    23581 aaaacaaatc cgtatacgtg aataacttct agaaataaaa gaaatttgca aaaccactcc
    23641 atttatctac ttctacgtgc attttttgat attgttttct ttatttaaga ttatacagaa
    23701 aaatatctag ggtcgctgga ttctaagtaa aaaaaatttt taaaataaaa agaactaaat
    23761 caagtagtaa ttaaactact gttgatttag ttctaataaa aagatttaga tgaacagttt
    23821 tattgattta aatagttcat attttttatt tataccgctt atttactact tgctgtacta
    23881 gaatctgaag tgaatgtact caaaacattt tcaaagtctt tgtctttgat ttttacatta
    23941 gcgtctttca atacttcgcc aattacttta ttctggaagg tactgtcact taattttgtg
    24001 tctgttgcaa tttctttcaa ttcttttttg tatttatcca tgtcgttgcc tttgttttgg
    24061 tttttcacca ttttaaccac ataatattcc gtagtataag ttgaagcatt ggtagaagtg
    24121 atcacatcag aaacttgacc atctttcaac ttaaaggctg cttctttgac ttctgccgga
    24181 accgttgtag tagtggaatc aaatttcact tttccgccat cttcttttgt cgtagtatca
    24241 gttgacttgt ctttggctaa tttagagaaa tcgtctccct tatcagcagc tttcttcaca
    24301 tcttttgcat catcttcact tgctacttgg ataatttgag cttccacttc tggatggaaa
    24361 gattcccaag ccgtttttaa atcttcatcg gtcaatttga tatgtttctt tagaccttct
    24421 tgaaaggcta gactttgttt gatactgtct ttgaatgttt gttctgtgta acctgcagat
    24481 ttcaattgtg aatcaaatga atcacccaat tgtttctttg tttgatcata ttgagaatcg
    24541 atttctttgg ttgatacgtc atctccatat ttatcttcaa atacttgata aacgatcaac
    24601 tgttgcacag cggaatctgc tccttgcatt tttttcaagc tatccattac ttcttgttcg
    24661 ctgattgtac cgcctttata actaacggct gttttttcac cggcattcgt acagcctgac
    24721 aataagccag tgcctagtac gatggcaagt gctaaaaatc tttttttcat ttatcaattc
    24781 tcctactttt cgttttttgc gaattgctaa aaacaaaggt gttcaagatg aaaatcatca
    24841 agaacacctt tactaatctg ctcgatacgt cctgaataga tataaggcta cacaatccag
    24901 aaagttcgct cagcagaata aatgtcaagt agcacatcac accttaatat actacttctt
    24961 ttgttaccgt ttttcaagtc tatcttgcgt tccgtattta acgtaactaa ttcttagaac
    25021 aaatgttatc gattttgttt ttcttcttcc gctgctggga tattcaaatc agacactttt
    25081 atgttcgatt ttccagacat ttttttgttg tttttattat gatatatttt tatacaacta
    25141 aaaatgaata tattaaaact accaaaaata acttttaacg ctgtttcatt atatgttttt
    25201 tctattttat ataaactttt ggatacttaa taccaaccgg ttggattgta ccaacatgag
    25261 aatgtgtcag ttctatttcc tgtcgcactt gcataggccc atactccttt tgttgcattt
    25321 gctgatcgaa tagagtttcc aactttcgct tttgcaccgt gacgattata gacacgacta
    25381 tagtggttag aatgacctgt ttttctcgta caagttgtat cactataata aacccatctt
    25441 ccagagctat gattaattgc tgttctagaa atttcccggt ctactggtaa atttgactta
    25501 ataatattac ctttagcatc aaaatgaatc tctcctggct ttaaagattt gtaatagatt
    25561 tcaccgtgaa ctgaatttgc ttccacttca gatgacatag cgtttcctcc aacaaaacct
    25621 atccctagtg cacaaataat aactgctttt ttcataaaac gacatccttt cctttttgat
    25681 ttttaaagca tgatcttctt gtttcctatt ttggtcagat atggatcatg cgtgactacg
    25741 ataactaacg taccttggga agcttgttgc gataagattt caactacttt atccttattt
    25801 cttttatcca atgacccagt cggttcatcg gctaaaatga ttttgctatt cttcaataag
    25861 actctcgcac aggcgattct ttgcttctca ccaccagata atgtgtagat aggcgtactt
    25921 agcttcttgt ttatatttac ttttttcaat gcctcatact ttgctgttcg cttatctgaa
    25981 acatttaaat tcgaaaactt caatccaata tctaaattaa aatcgattgt ttcatattca
    26041 atcaacccaa aatcttgaaa aagaaaagat atggtatttc tttttatcaa gcgcttctct
    26101 ctcccagtta attgtttcgt ctcttgatcg aacaaataga attgtcctga actgaactga
    26161 tcattcaatc ctaatatatt caataacgta cttttacctg ctccactttc tccaacaaca
    26221 gtcacaagat ccccagcatc cgcagtgaaa cgatccaaat gaagaatcgt tttgtttcga
    26281 atgatttttt tcgcaccttt tatcactatc aacggctcca attgttaacc tccattcatc
    26341 agcaataact gacttttctt ttctgtagac aaaataaaaa caactgaaag aataaaatca
    26401 atgaagctaa agaaaaaaat attaaaaaga tatttaggct ctctttctat aagaaaaagg
    26461 aagattacac ttgtcgttcc ccatttgatg aggttcatca tgaagaaatc tttatacgtt
    26521 tcaaagagcg aataaccact tgaacgaaag atagcgattt tccgacaatt gtagacaaaa
    26581 tatatgcata acatgacata gctcaacaag aagaaagaaa agaaagtgag taaaaatatc
    26641 tgtatcaagt aatgaaatga tctgcttaac actttgacag ctatctcttc agtctgagta
    26701 tacggaaatg ataactgcaa gttatcacgt aactgacttt cttcaatcag ctcaaataac
    26761 ctttctttcg acaaagagcc tatcttgatc tttaacggtg gatagatttc tccgctgaga
    26821 atatttcttt ccaaatacat actgtttttc aatgtcagta tttcaacaac cggataatgt
    26881 tcgatccagg ggatagaagg aataaatgta taaatgggtt gattgttttt tataaataga
    26941 aatttcggtt ctttttcact gatttcttga taatattctg ttatttgcgc caggtcattt
    27001 tttaaatctg tatgtctata tcgttcaggg atcaatataa cagggtgcgt ttctttttcg
    27061 gttatttgga tcaaccttcc agagacatca atcaaaggaa atttcttaag ataattcaga
    27121 tttattttta tccctctgcc aaactgttca ttttcttcat tcaagtaatc tgatacattg
    27181 actaatatac ttccgttttt attcaaagtt tcatataatt cctgatctgc tgctgatgca
    27241 aaacgattgg aataaataaa atccacgtgg tttttcccga tggttactgg ataaaataca
    27301 tgatagtcct caccagcgat ttcttttggt ggttcatggg aaacagctaa ctgtgaagaa
    27361 gaaaaaacga tagttgctaa ttcgacattg gcaacggtca aaagaagaga aaacaacaat
    27421 ccaaatgtaa aaagaatgta ttggatatag ttgaacgctt ctcttaacga agcgattttt
    27481 tcaactaata aaagagaaat caaagagata ccatatattc cgctgaacaa gaaaacgtac
    27541 atctggaaca aataaagaaa ttgctcctta taatacataa aaataaaacc accaatgatt
    27601 cctgcaatta tgaatgtaat aatgattttc tttttcccca ataaacacca caatacttga
    27661 tgtaatgaaa agtcattatt caacaataaa ctgatttctt tattcatcaa taagaaaaag
    27721 gatgataaca aaaggaataa aagaatcaat ccaatagtta aatacactcg ataattatat
    27781 acgtctgtat ttgaaataac cgaatacgaa tagctatttt caaagtcttc aaaaatatta
    27841 gtggcataat cagtgtgaaa tgcttgattg aatcgatcag tgaatagtga aatgacccgt
    27901 tcataggctt ctttttctct cgtcatcaag aaaaattcac cttcaaaatc ttttattttg
    27961 atcgattctt caatggacgt tacgcgaaag gaggtattag aatagggtac cttctcgtga
    28021 gaagacttga catccccttg ataatacaat gtcatttcaa gctttggctg tatcttgaag
    28081 ttccaagatg tatctacttc attggattca tttactgttt ttttcatata agaaagctca
    28141 gtttctgatg agacattttc aaataccatg attagctgct catattcttc ttgcccaata
    28201 aaatgttgtg gcaatcgaat cggagtataa gtatctgttt gatggaaaaa ttcatttgtc
    28261 cgatagagta cctgtttatt tatactaacc aaggcaaaac cactcatgat accggtcact
    28321 atgattgtga gtaaaactgt gaactttttt ttgattatca ccactctccc ttacttaaat
    28381 gattctttga taaaatcata ccgtttttca tcaaaaaaac gtatctatca agtgtatagg
    28441 tcatgtagaa taaatctata tactgtagaa tcgaaaggag aagaagatga acaccggaga
    28501 aaaaatacga acttaccgca gaaattgtaa gcttacgcaa aaagaattag gcgaaaaaat
    28561 tggtgtttcc gataaaacga tttctagttg ggaaaattct cgtacaatgc cagactttga
    28621 aatgctgtca cttttacatc atactttagg tctgccgatt gattttcctt ctattgaatc
    28681 agctaccgaa tcaatagaac caacattgcc agatgaacct cttgttaaca agaaatctta
    28741 ttcatcaaaa caacccttct cactcaaaaa gacactatct ttctttctgc tcgtcttttg
    28801 tttggtctat ctttttctta tcaattatca atacaaaaat gaaacgatcg atcgattcaa
    28861 tccttttttg aaaccacaaa tcggctatac cacactccct gtaggatacg tggacaattc
    28921 catcgaacgt tgggtatgta atgatatctt tggccatggt acatggctgt cttttcacgc
    28981 tggagaggta gaaaaagaga aaaaccatgc tgtaatcaaa cacaaaggaa aacacgtcat
    29041 gaacgttcgc ctgctcactt gggaagccat tccctcagat taccaagaaa tcctcagcca
    29101 agactatgac gacgccttaa aaagaagccc tcaggagcca gataggcatt aagtaggaga
    29161 tgaacgtaga gatagaaaaa atcaatgcac atgatcttat ccaaaaaaca ggggtacaaa
    29221 ttaattgaaa aagccttgag atgaaagttc ctaaataaaa aagttcagaa tgataggtat
    29281 ttctggctta tagtataata tttacgccac agcctcccaa tgattctttt tggaatcata
    29341 atcaaggatg gacattcaca aaaatatttt gctcattcga tccgaataga ttaacgacca
    29401 atctagtaag taatatagat tggtacgtag tcaagctgca cataatttag atcaatttta
    29461 gttctttttt atatttctat gatctgctca tttactttta tatgatggct tacagtaaca
    29521 taaataaata tgataatcgc tggtagttta tccaagagta ctcaataaaa tacaaaatgg
    29581 attgttcaag cgcctatcat aatgataaaa tgaaacaaat aaacgataca aggaggaaga
    29641 gactttgagt aatggcagaa caattttttc attgtaaaaa ttgttcagtt gttaatctat
    29701 gggatttcgg tagcatgggc atttttttat agaggaatca caccaacctg gccgttttat
    29761 ctcttgttag tgggaatatt agtaggaaat tttgcccctc ttcactatcg tacgaacttt
    29821 tttccaactg attctggcct ttttcttaaa acaaagacgc gaaaattaga aaacattatt
    29881 gaaatgacaa ttacctgtat aatcattttc ttagttatat tcctaaacgt cttctaataa
    29941 gaatctctta aaattagctt gttacagtta gttacctagt ttatataaca cagttatctt
    30001 atcttcctta tcaacatctt ttgccaactc tttgtcttaa attcgaaaca tataagcaga
    30061 gctacgaaca ccaagactgt ttcggctctg ttttttgcat caaattcact cttatatagc
    30121 tgataatctc ggctctttaa aagaaattat tttcgcttca ttttattata aaaaggctag
    30181 gaaccttgat gttatcaggt tcctagcctt tatttaagta ttaccccaca gacccttcca
    30241 tatagatttt taaattacac actagtttaa actaaaacaa acaaggaaaa acttaccatt
    30301 taatcaaaaa attagctcat atgaatatat tagaatgtaa attagactgg tcaaaatctg
    30361 gtcaaaaaag aggacgaata caggtagtcc tcttttttgt tattttgata ttctaaatca
    30421 ttttttattg gattattccc ttgttcgata tagacccaaa ttgatctatc aataattatc
    30481 aaactgagtc atatagagac aaaaatttca aaaatgacct gttaaatggt catcgaatat
    30541 catttaaaat caatacgacg gagtcagaca cggagtcaaa acaagcccgc tgagagtaaa
    30601 aaatatacag tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac
    30661 ctgaaatgat tccatttaat ttttatctca ttttttccta ctagttagta tcaaatactc
    30721 tcctattttc tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt
    30781 cattatagca aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga
    30841 ttccaaaaaa acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc
    30901 tcaaaacacg gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca
    30961 ttattcaata gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat
    31021 ccctattttc tacacttcca cttgactacc atctttgaat cgtacaattt tcttaactct
    31081 tctcgtttac ctcaattcat tctcaagtgg aatcaactca tcatctaaca ttttacgcca
    31141 tgctcgcttc atttcttgtg ggtatttaaa tggcttcgct tctggatgtt tggctttata
    31201 tccttcgtaa tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg
    31261 tagatattgc agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa
    31321 gacatgaatc aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc
    31381 ttcattacta tctaaagtcc ccacatcaaa tgcatttaaa atttgtcggt aaacttcttt
    31441 gactccttct gttttattca atttagccaa agctttttca atttcttctc gtgcatctga
    31501 actattcgca gcataagttc ctagtaattg atcaatataa gttgaatcaa tatcatacaa
    31561 cttagtggta ttttcactat aaaattcgat cgttgaaaaa tcttctcctt ctttttctgg
    31621 ttcttcctct attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag
    31681 ttgaatttga ttgttcaaag ttggagattt caccatgtca tcattgtaat cgtcataagt
    31741 cactaacgat tcataagcac gattcaattc ttgaaatgct ttgacgtact cgatacggat
    31801 ttcaagtggc gtatgttcat caatggattc catggatgaa acctctttta acttaccata
    31861 tgcttgtttg aatctttttt tggattcctg ataacttgga taaatcaaac ctgattcttc
    31921 ttttgctttt gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tcgctggctt
    31981 acgaaacgtc acaatcaatc cttttgtctt atcaggatac gtacgattcg tacgtgaaaa
    32041 agcttggatc aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg
    32101 ggcgtcaaaa cctgttaata gacgatccac tacaatcgct aaatcgattt gtttcccaaa
    32161 ttgtttgaat tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc
    32221 aatgtctgct aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg
    32281 catttcttct tgttgggcac ttgaatctcg gctattttct tctaatgaat aggtaatagc
    32341 gattcgtgga aaatcaggat cctccatcgt tcgtccttca cgaatgagct gattgggaaa
    32401 ctcttttgtt aaccaatcag gctctttcgt catctctttg atggcccgat aatacttttt
    32461 ggccatgtca atggaactcg tggttaagat tgcagatttc gttggcctgc catttcgaaa
    32521 atcaaatttt gtataggcat tatctggacg gaacatttta tgaatgactt tttggatatg
    32581 ttcatctgct tcaaaaacag ctgggtccaa ataactttct tttttgacag gttccatttg
    32641 atcaatcata cgattgatct gttctgatga atactctgca tatgtttcta cttcttttaa
    32701 tttccgatag attttattct ctagtgaagt cggctccacg gtgttttcat gctctacttg
    32761 aaatcctaag acagagccat cttctagcgc attcttaatg gtataggtat gcaagacctc
    32821 tccatactga tcatgggttg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg
    32881 aaaaatcggt gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc
    32941 ttccatattt tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg
    33001 gcccattaat ttttggaagc gattggttcc ttttttctct tcttgtttct ttgcatattt
    33061 caacgccgca tctagttttt gacgggttgt aatgattaca acatttgcat tggcatctgc
    33121 caacagggtt tcacttaatt ctttggcact tcccgttcca acgatcaagc tattcgcttt
    33181 ggcgttcccg gaagaaatcc cagtattaaa ttccgaagca aattttgtaa actctgtggt
    33241 ggtttgatta tctaaatctt tgcggtccac taacatgatc gttcgatcga tccccgattt
    33301 tcgtgctaaa agcttagtag atacaaaact agtcaacgtc tttcctgaac cagtcgcatg
    33361 ccacacatac cctgattgat gtttatttgc ggcaataaac aaggcttgaa tcgcatgtac
    33421 ttgataagga tgtaacacca tgagggtttt attatcttga tcttcactga cgattgtata
    33481 atccgcaatc aaacgatgag catccgggat atttaacact tgtttacaaa actcatataa
    33541 gttttctact ttttcattgt ctttcgttcg ccaactaaat aaaaacttag ggtgcaagtc
    33601 tttcggtaag gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg
    33661 taaggtcgaa aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt
    33721 aaatgcctga taaacaccat cttttgcagt tacttgtttc agctcgattt ggacaatcgg
    33781 taatccattg attaataagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc
    33841 acgttttgca atttgatgga ccacttcata agtagagatt cctccaccaa tgtcttgatt
    33901 ggaataaagg ataagtgaga cagaacccaa ttgcgaatct tcccgttcaa tcgtgatccg
    33961 agccatccca ttttcacctt ttaaccattt cgcagcatca aatggggttt tcgttcgcag
    34021 cagtaattcc gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc
    34081 tgcttgatta tttgagataa ttttttggcg taaattgacc caaaggtctt cttctgattt
    34141 tagatccgga cgatacgtcc actgattatg cccttctcca agtacacgaa tcaactgttc
    34201 ttcgacttct gcttcctcac ggtgactaat tttcattctt cttccctcct agacaaacat
    34261 cttttgtaag aagctttttt tcgtttcttt tagtaaatct aacttacgtt gatgaagagc
    34321 gatcgtatca tctagctgtt tgaagaaagt accgattttt tgttgttcat cctttacagg
    34381 aaccttaaca ctgatattct caatatcaga actgttataa gaaggcaatg cccctacttg
    34441 ggagtatctt ggcaaattta tagtttcaaa caatatttta ccaaaataga tatcccaagt
    34501 attattaaat aaataagcca tggtattgtt atctattaag aaagggcttg tgaccaaacg
    34561 ttttctatta agcattattg ctgcgccaac tttagcaaat ataatcgctg gcacatcttt
    34621 tattggcttc cattttttac gctcaatttg ctcattagat acataatgat ttgcatttct
    34681 catttcatac tcattaccaa tgttattcat gtcggaaact ttaaaaaatg gagtaccttc
    34741 agaaccacct tgctcggcat caggaaatcc aattcccgat tgagttttac ctaattctcc
    34801 caacttacgc tcttcccaat cttccgtaaa accaggaaac cggatttctg gcacttttgc
    34861 tccgttttca gggaacattt tttgtaagaa tccttttttc gtttccttca gcaaatctaa
    34921 cttacgttga tgaagagcga tagtgtcgtc gagttttttg aagaatatgc cgattttttg
    34981 ttgttctttt aaatcattgg gaatatagaa tactgtatct gaaacttttt tccagtctga
    35041 acgaggcatt tttgttccag atgtatcatt tgcagccttt tgatatctat cagcttgaat
    35101 aagagaataa ataaagtccg agtcgctctt cttagctcta aaaacccaga aatccccaat
    35161 cgctacgcct gtaaaagttg ccttgagcca attttttaaa tatggacgta actttccata
    35221 tagaatatca tttggtaaaa attcaatccc ttttctatta tcaaatttct ggctcacatc
    35281 tttattcagt ctaccttctc ctgccacgat atcttcaaat tcaacttttg gaagtctact
    35341 ggagtttgta cttttattaa gtctaacaac taaatcaatt aacttacgct cttcccaatc
    35401 ttccgtaaat cctgggaaac ggatttctgg tcgtgtatca ttgctcatgt ttgaacacct
    35461 ccagcgcacc ttgtaaccac atttcgtttt caggtgttgt ttgcagggaa gagatagctt
    35521 ctaaaagttc tttttctagc acttgctgtt cttgtcgtat cttcttgatg tcatttccga
    35581 catgaaccat atcaattggt tcttcttctt cgaaagtatc tacatagcgt gggatattta
    35641 agttatagtc attttctttg atttcatcaa aggttgccaa atgagcgtat ttctcgacat
    35701 cttttctttc agcgtaggtt tcgaggattt tttgaatgtt ttcttccgat agtttatttt
    35761 gatttttccc tttaacaaat tcacgactag catcaatgaa taatacatct cgagtttgac
    35821 gattcttctt caacacaatc accgtcgttg gaatcgatgt cccaaagaac aagttcgcag
    35881 gcataccgat cacagcataa atactgccat cttctagtaa tttttgacgg atgacccctt
    35941 ctgcagcgcc acggaataaa actccgtgtg gtaagacgat ggccatggta cctgtttcct
    36001 tcaaatgata gaacccatga agaagaaacg caaaatctgc tttggatttt ggcgctaatt
    36061 tcccataacg attaaaccga gaatcatcta agaaagtcgt atctgctgac caattagccg
    36121 aataaggtgg gttcattact actgcatcaa atgtgtaagg ttcatctgtt ggccagtcct
    36181 tattcaacgt atccccattg cggagattca tttcttcggc gtcgacacca tgtaaaatca
    36241 agttcatttt tgctagattg tatgtcgttg tatttagttc ttgcccatga tatttaacgt
    36301 tatctgggtg tgttaagtag ttacgtacgt ttaacatcaa tgaacctgaa cccatcgttg
    36361 gatcaaaaac actaaagaat cgtcgttcct tttgatcgag cgttacgatt tgcgccatca
    36421 tatcagaaac catatgaggt gtgtaaaatt ctccggcttt cttccctgct tctgaagcaa
    36481 attgactgat cagaaactca tacgcatccc cgatcacgtc cccatcgtgt tctaaaacat
    36541 ctacatcatt caatttcttg atgacttcgg taatcgtcac atttcgttgt tgctcatctg
    36601 ttcctagttt ttttgattgg agatccacat catcaaataa cccattaaat tgattgtagg
    36661 tagatgccaa ttgcacaaag gctttgttta gatcgttcaa ttggaacgtt gcttgttttg
    36721 cttgatccgc taagacgttg aaaagatact ctggtgcaat tgcatatccc aaaatatcaa
    36781 cgattgtggc aatcagatct tcttttgatt cttcatctga aagtaattca cgatacaaca
    36841 ttgtttgttt gcttactgtg tcatattcct ctaaggattc atctgctaaa agaaccactt
    36901 gttccaacaa tcgatcggat aaatatttat agaaaatcag tcccaataaa taatttttat
    36961 attctgacgc atccatttta ctacggagat tatctgccgc actaaataat ttttgattta
    37021 attctgcacc caaaaagata cactcctatc tcttaattaa agttgctata taccctctta
    37081 ttttagcata ccccctttta taaaaagcaa aaaaaaaaga aagaaactta ttcttgctaa
    37141 taaactacgc gaaaggagtc taacgttgtt tgatataact tagactgatc gttaacttat
    37201 tttttatatt tctacatagt tatctttaga accatttcag atacatatga aacaattaca
    37261 tatgattttt tcataaaaaa acagtactgt ctagtagtat aaaatataca aatttcttaa
    37321 aaattttttc actaaaatca ctacctgaaa cagtctaaaa cacttatata tcaacgttta
    37381 atgaaactat ctccgtttca tttcagatac aaataaatat agtcgggctc caaatccgaa
    37441 aaaaaatacc cccagtagct tatcctaagc tactggagat gtataagtac ggtcatataa
    37501 caacggggta gggtaaataa atgaaaaaga tggaagaatc attattcaat aactttttta
    37561 aatacttcgt tgtaatcagt tacctgtccg ttcttaaata ccacagaata atctttttac
    37621 ccattttcaa gtaaccgaag tggaatgtct ttaccaactt gctctgtcac tgcattactg
    37681 atacctgtta gattagctaa taaatctgat aaagattgag catctgtaga aatctctgta
    37741 tcagtattta tcgtgattcc tttatcttca tttacaagct tccaagtagg tggtatagct
    37801 gtgtatgttt cgatgaatga atcaattctt tcatctatcg tcttcccagt atctacttct
    37861 gtctcattac tcttagatga atcgattctt tcatccatcg tctgctccgt aactgcttct
    37921 gtctcattat tctcaccgga aactctcttc acacccaaac ttatatattg tccatcttta
    37981 gtgtgcatag ttaaaattcc cacgctttta ctaataaatt cactataacc agttctttca
    38041 atagagactt caatgtattt cacactatca tactcaaaag aaaaatagac atgtaaaagc
    38101 attttgttgt ttacatctac ataataatcc tttgactcta cttgagacct tcctatacgg
    38161 aatacagctt tactgtctga tatctctata ccagcatcct tattattaat ctgccaatct
    38221 ccaagtagct cttctggcaa gctagtagtt tcttgtgaac actttaccac ttcactggta
    38281 gaggtcgaaa cattcacctc cgcactgtta gaagaatttt ccaaggaatc aggagcctta
    38341 gcacaaccaa caagtcttta tatattgtgc agccgatatt ttcttcattc agtacaccct
    38401 tgaaatatgc agtgttagat aaagcagttc gtcctgattt gctgaggcac ccatctcttc
    38461 ttgaacgtac tccttgattt tcaaggccgc ccgatactct ttcggaaaac gctgcttcac
    38521 tgaagccagc aaaaaatcat ccttctccgg caactcttcc ttcttaatca ttcgggcaat
    38581 aaaaaactgt aaatgcgtca caaatcttaa gtaaagcatc gaatctttat caaccttttt
    38641 ttcgggaaac agatatccta gcatagccaa gatatccttt agtgctctta ccgagagcat
    38701 ggtttcttcc atatttgctt gttcactttg ggcatttaca atgtgaaacg caatgtttcc
    38761 agcctcctct tctggcaact gaacattcat ttttttgttg atcaattgga tagcatagca
    38821 gccgatctca aattcttttg ggtaatactt ttgaatttca atcagcaggc gattttgaaa
    38881 aacaatgcct ttttgactgc gttcaatggc atgcttcaag tgatcaacca aggtaaaaaa
    38941 tagactttta ttcagcttaa agtcaaactt ggcgttggca taatcgataa ttcgcttgac
    39001 gcaaataatt agatcatccg gagcagcctc aaacagttta atgtaatcct tttttagagc
    39061 atcagaaagt gaatctaatt taaatacttt ttggattttg tcagccggaa ccaaatcgcc
    39121 tttctttagt tgaaatccaa tccctttccc catcaaaatt acttctttgc cttgcttatc
    39181 taagcaaaga attaaactgt tgtttaacgt tttaattact ctcattagat tcaccgttct
    39241 ctctaagcta agccatttct tgcgattact ttttgatacc aaaagaagct ttcttttttt
    39301 attcgtttta attctttaag attctcatcg gttcggtcaa catatataaa accataacgc
    39361 ttattgaagc cttgatgggt actaacaaca tcaatcgctg accaaggtaa ataaccaaaa
    39421 acatctacac catctgttaa agctgcttga atagccagaa tatgtttggc tagataatca
    39481 attcgatcct ggtcattaat tcgtctatct tcctcaacta tgtcgggtgc cccatagcca
    39541 ttttcagtaa ttaaaatagg taaatcatag cgatcgtaca atttcctcaa tgttaatcgt
    39601 agtccagtag ggtcaactac ccagccatat ggcgttttat ctacataagg attttcagct
    39661 gcccggtaga caccttcttc tcccaacatg atctgttgat cccccgctct agcggaaaca
    39721 tctgatccgt catttttgct ggcagcgatg gtggcagttg aataataatt aatcgcaata
    39781 aaatcgggtt taccggagga taaaattgcc tgatcctctg cctcaatttt aggatataat
    39841 ccacgatcat tcaaatagtt ttcaaacaaa taattgtatt ttccccgaac tgctacatct
    39901 aagaaagacc agcctctcaa tgtttcccaa ttatgggctg caattgcatc ttctggactg
    39961 gaggtagctt gatacatagc cgttaaattg attgctggac cgatttttcc tttaagattc
    40021 aactgatgat acaagtgaat tactctagct tgagctaata aaacatggtg attaatttga
    40081 aagagttctt tttttgaagg cagctctcca cttggtggca aaccaatcgc tccaggatga
    40141 aggatcatcg tgttttgctc attaattgtc agccaatagt tcaccttgtc accatactca
    40201 gtaagcaata cctgtacata gtccacaaaa taatcgatag ttgctcgatt taaccaaccg
    40261 ccctgctcct ctagaaaata aggtaaatcg aaatggtaca tggtgacaat cggttcgatt
    40321 ccctgattca ggcattcatc aattacttga tgataaaaag aaattccctt ttgattggtt
    40381 ttattatttt caatatccat aattcgcgtc caagcgatgg aaaagcgaaa agcttttaag
    40441 cctaattctt taaacagctg tatatcttct ttatagtgat ggtaaaaatc cgaggcaact
    40501 tcaaaattag ctaccccttc tgtatgctga tacttgtcaa taatagacgg ttgtttccca
    40561 tcctcctgat aggcaccctc tgcctggtag gcactaatgg aggcacccca taaaaacttc
    40621 tctggaaatg ctgaaagctg tttgtattgc acaaaaatcc tctccttaac taatttgcat
    40681 caatggttcg tgttccttta agcttccgct tgcttcacta attcctgcgt aattttcact
    40741 gttggtcact accacaatca ctgtatcatc gtagccagcg tttctaatgg cagcgaaatc
    40801 ggcgttgatt aataattgtc ctgctgacac ctgatctcct gcttttacaa atacttcaaa
    40861 gggttcccct tttaaattca cggtgtcgat ccccacatgg atcaataatt caataccttc
    40921 tttagaacgt agaccaatcg catgtttaga ttctaaaata gtaatgactt ctccctcaac
    40981 aggtgaataa atgctgtttt cttctggatg aatggctacg ccctttccca atagtccctt
    41041 agaaaagaca gcatcgttca cttcagaaat aggacacacg gttcctttta ccggattttc
    41101 aacaatgact gcttcggcta ttgccgtttg ctctgattca tcagaagtat cgtcaaatcc
    41161 aattagataa gcaatgataa aggttgcaac aaaggcgatt acacacgtaa taattgcgtg
    41221 aacaatattc attggatttt ctccaataaa ggctggtaag gctgctaagc caggggaaac
    41281 aaaggcatat ctgactaaac cgctcaagcc agcatacaaa ccagcaattg caccaccaat
    41341 catggaagca actaatggtt ttttcagctt caaggttact ccatagagtg aaggctcagt
    41401 aattcccatt aaagcagtga tcccagtgga cacagctagc tgcttcaact ctttgttttt
    41461 agttttcgcg gctacagcaa aggtagcacc tgattgagca atattagaag ccagcattcc
    41521 tggcccattg atcatttcag aacccgtact gctcaattgc atagtagcga ttggcgtcat
    41581 tgcccaagct gtacccgtaa ccaccaaaaa tggctgcaaa ccacccatca aaaatggaat
    41641 taaccaagct gctttgccgt taatccattc agcccccatt gctataagat cgttcagcaa
    41701 cgttccaaac ggtccaatga caaccaacgc taaaggcccc ataattaaaa ataccagcat
    41761 cggcttagta aagaatttaa taaacgaagg cgaaattcga tccgcaaagg gctctacata
    41821 ggacatgatc caaacagtaa agataatcgg taaaactgat cccgcataat ctgcagctaa
    41881 taccggaatt cctagaaaag aaacggcatc gccagtagaa aaaagctgtg aaatatttgg
    41941 atgaagcaaa atgccagcaa cagtaatcgc caaaattggg ttgacatcaa actttatggc
    42001 tgccccatag gctaatagta ctggcataaa ataaaaggct gcatcagcaa tagtattcaa
    42061 aatgatgtaa gtttgactac tgtctgatag tacaccagtc aaagtcaaga cggccaaaat
    42121 ggcttttacc atcccggcac ctgttatagc tggaatcatc ggtgtaaagg tggtagaaat
    42181 gacactaatc aatcttgaaa taatactttc ttgattgtct gaagaggggc cctttttaat
    42241 tgaagttccc acttcctttt gaatttcctt gaaggctgtt tggacttcat tgccaatcac
    42301 cacttggtat tgtccaccgc tatccaccac actaattaca ccaagtgttt tttctaaggc
    42361 tttcgtatcg gctttccttt tatctttgag ttcaaaacgc agtctggtgg cacaatgggt
    42421 taagctgacc acattttctt ttcccccaac ctgttgcaat acatttcttc ctacttctct
    42481 gtaatccatt attctctctc cttaaagcag taaaaaagac caagtcaaac cacgccccaa
    42541 tcaacattga agtgttagtt tgtcttggtc ttgcctgcat aatcagtaac atgccaataa
    42601 cgttattttg ctgtaatcca ataatagcac attatgatag cgcaatcaac agggaattta
    42661 attttttatc ttttcgtttg accgtatagg gtaatataac agttagatca catcaactag
    42721 ggtatatctg cgtatactta ctgctaaaat aagtatatct aatattaact ataccagtaa
    42781 aaccattcct acataaccag cgggaaattc ccaaaatcgt gaaagcatca aaaatgcata
    42841 taacggatca cacaacttca acaattctgc tcttgggaaa aattgatgaa tttggtgagt
    42901 aaaggatgga ataaacagca ttccaacaat ataggcaaca atagtgatac ttgttttttt
    42961 ggtaaaaatc agtaagcaat agccaatttc aatcattccg agatataaag gtatcaatag
    43021 aatgatttgg gctagtattc ttaaaaatta actagcaaat gtttaaactg atttatttct
    43081 aatctacatg tagtaagtat ttcaattcca ccaattatca ataaaaatac caaacatatc
    43141 cataataatt ttttcttttt catttctttt atattgttat aacagtgaat aacataaaaa
    43201 ttcccgacaa tcattgtaat atttttttgt cacctgtatt agtatttaat ttataggaaa
    43261 ctataaaaac tccctagtga atttacacct ttgggagttc ctgtttggac ggtatgcata
    43321 atacatttaa atcaatttaa cattaacacc tcagcatatg gtatctccac atatatacgg
    43381 tctttctttg gatagtactc aatatttatg ttgatagcac ttccattgtc taatatagct
    43441 ttagcccggt atgtgttatc tccattttca tgagtaatcg tcacatcatc acatgatcga
    43501 tttaagttgt actgttcttg taaaatctgt gttaccaatg gttttgatga cttctctaat
    43561 ccagatgtac ctgtcatttt tgaaaaaata atacctataa tagccaagac aacaatacct
    43621 ataatgacaa acttcttgtt catatttact ttattcaact ttttatctcc tatctccaaa
    43681 ataatttgtt cttatccctc ttatcagtac attgacatcc ctaaaagtac accaaagatt
    43741 gctccagcta cccctgctat catcaaaatc gtacccattt tctcatcttt ttctcgatat
    43801 aagtagccca taataacagc aacagcacca aatacaacag gaataaatgc taacgatact
    43861 accatagaaa tccaaccaat aatcaaatac agcttccctg tctcttgtcc atgtacgatt
    43921 tgttccttca tttcattaat tgtctgctca gtacttttct ttactgtgtc tgttccggtt
    43981 gtctcatctg tttcttgtag tgctccacaa aactggcaat acttactttc atctgttatt
    44041 tctttgccac attttctaca atacatgtct tctcctccta aaatcattca attgcttatt
    44101 agttaactgt ttactttatt aaatacatca gaaaacagct cattcattgg tacacttaat
    44161 atctcagaaa gcatcttcat ttgatagggt ttaggaatcg ttctccctgt ttgccacgaa
    44221 cttacacatt gctgtgtaac attcattttt tttgctaact ccgtttgaga aagtcctttt
    44281 tcttttctgt atcggctgag attacttact tttttcatga ttctcccctc ctgttttatt
    44341 atatacaaca atatgatgta tttcaactca taaaatataa aaaacttcaa ataccattac
    44401 aaatacaaca acttgttgta ctatactgag atgggggtgt attaatgaaa cagaaacaat
    44461 atgcaaaggt cattagagaa aagcgaaaag ctctcgggtg gacacaaaaa gaattagctg
    44521 aaaaaatctt ctctacacaa caggcagtag caagatggga aaattctgta acagagccta
    44581 acctcgatag cttaacagct ctttctagag ccctaggaac ccctgtaagc cattttctag
    44641 acaatgttgt cgtagattat gaggaagagt ttttggctct ctatcgttct cttagcactg
    44701 aagatgcagt tcgaacaatt gactatatga agctattaaa aaggcaagaa aatgaacgca
    44761 atcagctatt gaaagattga aaaataaata acccaccgaa ctttaacggt gggtgtattg
    44821 agatgaaata agattaagag ctatggataa cctgtctagt ttaatacaat tgggtctttt
    44881 ctcttcctta ctctccgtcc atcatctctt gttacacttg taaattgata cagaggaact
    44941 atcccagctc ctgataatat caattctttt attgtttcat ctatccctgc ccctttcgct
    45001 aaatatatta aaaaactact acttgctttc acattgtata gtttcttaat atccttagaa
    45061 gctcgttcaa gagtgaatcc ttgtttaaca aagtagcctc tgttttttat taaagcattt
    45121 ttcacgctgt tatccccaac attacgtagg cgttcacaaa cagtgttaat aagttctttc
    45181 tctgtcatta gatatgtccc ttgccacgat aagccctgta aaacttcatg tgcttcatct
    45241 aaaatcaatt gtttttcctt gctgagttct gatcgggaat tttgaggaaa tactctttta
    45301 gcctcttttt cgttaaaaca tatcttaacc cattcataag aaaaatattg atatgtaaaa
    45361 tgaatttttt ccagaagtgg aaggatatct tcattcaatt tttgaatcac agaagttgca
    45421 ttaaaaacgt ctaatcgata gacattgctt ctcttatgat taaagtttcc taacgctttc
    45481 tcttccttta acctatttag gtattcgagc atcttcaaag gaacatcttc atcatctaat
    45541 ttttttatca atcctataaa agtcataagt gatagtaact tattacattt atctatgtca
    45601 taagttttac catagtaaga actcattttt ttagctaatt gttcccccga caaaatacaa
    45661 gtcacacttt ccttactatc atcaaaaaaa ttaacatcag aaagcaagac atcatagaca
    45721 cgtcgcattt cctctagata attccttatt gtcctgaaaa gtttgtcttt cattgattca
    45781 agagtgtcaa aaagtacttt tgattcctct ttcaattttc gaaaatgtgt aggatacaag
    45841 tctaggttct ctaatagcca attaaatgtt tctacccgac tgtctttctt taaaattagc
    45901 atcacaactc caataaaatt ttcactgata tcacaattac tggaatgaca ataaaaaatg
    45961 gaatgcccag ttttactggt aaaaatacta gcagagggat tatgatcgtt gtgaaacaga
    46021 catcttaagt ttttacttga cttaagtaat ttattcatat caatggttaa caatctttca
    46081 taaatctcat taaaagacat gtcggaggaa tcaaacaggc agtctttaaa ccactgtttc
    46141 atttcttctt tgtcgtcatt ttttatcaac ttaacaaaat cggtttcatt gactagttca
    46201 tactctctcc ctgaatcact ttgacaattt ttagaacatg tcatggtaaa atcaatcgga
    46261 agctcagtaa tatctaacat gttgtcttca ttaattatta tcactgcatc tgacttatat
    46321 cctccaaaaa acattctatt cgaagcattt acattgtcat cacacccggg gatgttcttt
    46381 ttcaagtatt cgtaaacagc aaccatttta ccatagtcag taattgcttc ttttaataga
    46441 aagacaatct tgtatttttc tagattttca gagtaccgaa tacttttttg tatcgcaaac
    46501 gcagaattat gaactaaatt gatagataaa accttttcta tgcttaagta tctactatca
    46561 tctttactca aatctactac cttccccttc tcatctttaa cagtgttatc gacatccaga
    46621 ataattactc tggtttccac aaaactctcc ttgtcattgc ctttattctt tgtcttgctt
    46681 ggaataaatg attgtgatga atttagtatc tctacaaact catctcttgt aacttctcta
    46741 tagttatcta aacacctatc tcttacatca gttttttctt tttttgtaac tggcttctct
    46801 aataattttt cttgatctac ataaattgga atattaatca ttgatgtttc catttgtgtt
    46861 tcctccttat aaaattctgt taaagactaa aacatacaga taataaaaac aaactcttta
    46921 ttactatatg ttttagtctt atttgtttac tcaaccttta taccgttcca tgaaactatc
    46981 aatatcagat tttttaaatc taatgaccgt atcaattttt ataacaggta acccgtattg
    47041 agaaacatat tttctaatcg ttcctactga tacacctaga tattcacttg cctgctgata
    47101 gtccatatac tctagagtga ctccagaaat aatgtcttct agcataggct ttagcactgt
    47161 aattatctct ttcacttgtt gttcaacttg tatagaaaca atgctatcaa tgatttgtag
    47221 agacatattt ccccctcctt tcgtgattgc ttgaacaaat tttctaagaa ttatcttcgt
    47281 attttttgct caaaataaaa gtattttgtt attaaccgat taatcaaaca aaaaatgatt
    47341 accagttcat cgacatacac ataatacctc gataaaaaag gggtgtcaac agctttgtaa
    47401 aatttattta caacacccct tttttgaggt atactaattt taggaggtgt ggtcaatatg
    47461 ataaaacgtc caccaataaa ttatttagaa cgaaaaaaga tactcggaac aaaaataaaa
    47521 gctataagaa agagtaaaaa attaacacaa ccagcatttg gtcttatgat taacaacggt
    47581 caattaattg acaaaaaaac tatctacgaa tgggagaagg gaacctattt accaatacca
    47641 gagcgattat cccgaattgc tgatttggga aatatgagta tagaggagtt agtttgtggc
    47701 aatgtagaag aatatatttt gggaattatt ctttatagag atagcattgt actagatggt
    47761 ataacatttc cagataaaaa cctgttccag catcttcgtc aacagttccc accagtacac
    47821 tctaatttag atacatggct ggatagatat tccaaattag aaccagagat gcaagaattt
    47881 attgctaaca agacttgtaa taaagttaaa aatgaaaaaa ttagtctttt caatatctta
    47941 aaaatagaag aattattcat caatgctatt gtagaagaat ttgacaataa cattcttttc
    48001 ctcactagca gtatagaaga actactagaa agaatggtag atgaatggct acctattcaa
    48061 ttgaaagaca tgtcttatcc cgaagaagct gtgagagaaa ttactgataa tataaacaag
    48121 ctagaacaaa cgatttcatc aattgggaaa aaatacacta agaaaaaaat gaagggaggt
    48181 gataccatct agtggtaagt actccttgta aactaagagg agatgattta atggcaaaaa
    48241 tatcaaagta caaaaagaaa aacggtcaag tggcgtggat gtataaagga cacattgcca
    48301 ccgatccacg aactggtgaa aaaataaata ctacgagaag aggttttagt agcaaacaag
    48361 aagctcaaaa agattatgac gagtacagac atcaaatatt atacggtgtt aaaaagaaag
    48421 cacctgatat gacatttgaa gacctgtata atgaatggat aactcatcag cgtaccagtg
    48481 ttaaagcttc aacaatcgca atctcagtaa gatacgcaaa taatcaaata cttccagcat
    48541 ttggtaagct taaactatcg aatatctctg tcccatattg tcaaaaagtg gtagatgaat
    48601 ggcacagtaa atatgaaagt tacgattata tgagaaaaca gactgcacaa attttacgct
    48661 acggggttgc aatgcaatat attgacaata atcctatgga aaaaacactt cttccacgaa
    48721 aaaaagagta tgagaaaaat cgtaaatttt actcaaaaga ggagcttaat aatttactgg
    48781 atgcttttaa agattttggt aacatgaaac aatacgcctt cttcagatta ttggcataca
    48841 caggtatgag aaagagtgaa gtactcgctt tacagtggaa agacatagac acgtttaata
    48901 aagaactaca tgtcaataaa actcttgctg tagatgaatt tggtaaagtt ataattcaat
    48961 ctccgaaaac aagagcttct agacgagtta tctctttaga tactgagact ctttctatcc
    49021 tcaacaactg gaaattacag caaaaagaag agtatttaaa acttggatac aatactagct
    49081 ctaaagagca acatgttttt acaacagtta aaaacactct atacattcca aacactgtta
    49141 atgattggtt aagatatatt ttgaaaaaat ataacttgcc tcgtattaca cctcatgggt
    49201 ttagacatac acatgctagt ttattattag aagcaggaga atctgtcaag gtagttcaac
    49261 aacgattagg gcatgaaaat agtaaagtga cattagacat ctatgcacat attacaaata
    49321 atgcacctaa gaaaacaggt caagactttg ctgatatgat ggctcatcag taaaagagct
    49381 ggtcaaaatc tggtcatttc tagtatacag gcaaaacaaa aaagctagaa cccttatata
    49441 tcaagggttc tagctcctat aacaggttta cccaacactg ccttccatct cataactgat
    49501 caatcgattg agttcgactg catattccat tggaagttct ttggtgaatg gttcaacaaa
    49561 gcccatgacg atcatttcag ttgcttcaga ttcggtcagg cctcggctca tcagatagta
    49621 gagttgttct tctgagattt ttgagacttt ggcttcgtgt tctaaagaga cttggctgtt
    49681 gtggatctca ttgaatggga tcgtatcgga tttcgaccat tcgtccatga tgatcgtgtc
    49741 acattcgata tgagagatcg aacccgcact gtttttggca aaggttactt gtcctcgata
    49801 gtttacttct ccgccgtctt ttgaaattga tttagagacg atagagctag atgtatttgg
    49861 tgcattgtgg atcatttttg cacccgtatc ttggatttgg tttgctcctg caaaggcgat
    49921 ggagagcatg gtgcctcgtg caccttttcc atctaagtaa acacttgggt atttcatcgt
    49981 tgtttttgca cctaggtttc cgtcgatcca ttcaacggtt gcgccttcgt atgcttttgc
    50041 tcgtttggtt actaagttgt acacattgtc tgaccagttt tggatcgttg tgtaacggca
    50101 atacgcatct tttcttgtaa agatttctac gatggcggca tgcaggctgt tacttgaata
    50161 agtaggtgcc gtacaacctt cgacatagtg gatgcttgct ccttcgtcaa caatgatcag
    50221 cgtacgttcg aattgccccg tgttttccgc attgatccgg aagtacgttt gtaatggaac
    50281 atcgactttg acgccttttg gcacgtaaat aaaggtccca cctgaccata cggctgaatt
    50341 cagcgctgct aatttgttgt cggttggcgg tacaagtttt gaaaagtact ctttgaataa
    50401 ttcagggtat tctttcaaag cagagtcagt gtcagtaaag acgattccta acttttcgaa
    50461 ttcatctttc atattatggt acacgacttc tgattcatat tgtgcggaag caccggctag
    50521 ataagctcgt tcagcttctg gtataccaat cttttcaaat gtatctttga ttttttctgg
    50581 tacgtcttcc cagtcacgag cgggtttgtc acttggtttt tggtaatatt tgatggcatc
    50641 gaaatcgatg tctgaaaggt cggggcccca tgtttgcatt ggcattttgt taaatgtttc
    50701 tagagatttc agacggaatt caagcatcca ttctggttcg cctttgattc ttgacatctc
    50761 acggatcact tcttctgtca agccttctcc tgtactgaaa accggttcga catcatcgtg
    50821 aaacccgaat ttatattctt ctaattctgg tacgcccatg ttctcactcc tcttccttgt
    50881 ggtaatgatg gaaagctgcg gtaccatcgt tttccattgc ttttcctagt gctttccagg
    50941 caagtgtcgc acatttgatc cgagcgggaa atttagctac accaccaagc atagcagcat
    51001 cgcctaattg gtcgggatct ttgacttctt ttccttggac aagctgtaga aagtctacag
    51061 ttaaatgttc tgcttcttct attgattttc cgatgacggc gtctgtcatc atcgaggcac
    51121 ttgccgtact gatggagcag ccactgccgt caaaggcgat atttttgatg attccgtctt
    51181 ttacatcgac ttgcaattcg atcacatctc cacatgtggg gttgttcaat tcgatcttgc
    51241 ttgtcgagtc agtcaagctt ccacgatgat gcggatggga agaatgatcc aagatcactt
    51301 gacggtataa attgtctaat ttagatagtc ccatgttgga aaaactcctt tgttgccatg
    51361 attgcttcaa ctaaacggtc ggcgtcttgc gttgtgttgt agaaataaaa gctcgcgcga
    51421 gcagtcgctg atacttcaag gtacttcaat agcggttgcg cacaatgatg acctgctctg
    51481 acggctaccc cttccatgtc taaagctgtc gcgacatcat ggggatgtaa cccatccaaa
    51541 ttgaatgcaa cgactcctgt ccgaaaattc gggtcttgtg gaccataaac agtcaatcca
    51601 tcaattgctt gtaattttgg caacacataa gcggctagtt cttcttcata tcgatggata
    51661 ttgtccatac cgagtttttg caaataatcg attgcatggc ctaatgcgat agcgccggcg
    51721 atatttggtg tccctgcttc gaatttccaa ggtagttctt tccatgtact gtcgtataat
    51781 tcgacaaaat cgatcatttc gccaccaaat tcaacaggtt ccatttcttc taaccatttt
    51841 cgtttaccat ataaaacacc aatacctgtt ggtccgcaca ttttgtgtcc gctgaaagca
    51901 aagaaatcac agtctagtgc ttggacatca atggccatgt gcggggcaga ttgagcacca
    51961 tctactacga tgaccccgcc gttttcatgg acaagttcag ctaattcttg aacaggattg
    52021 aagacaccta agacattgga tacatgagca atggagacga ttttggtttt gtccgtaatc
    52081 tttttcgctg catcctccat atccaggaag ccttcttctg ttagttcgat atatttcaag
    52141 acagcacctg ttcgttttgc caactgctgc cacgggataa cattagaatg atgttccatg
    52201 taagagagca ctatctcgtc tcctgctttg atatagcggt cgccgaaggt tctggctacc
    52261 caattcaagc ttgtcgtcgt accacgagta aacagcactt ctgctgtttc ttttgcatgg
    52321 atgaacgccc gtactttttc tcttgcttgt tcataatcat gggtcgcgcg ttctgctaac
    52381 gtatggacac cgcgatgaac attggcgtta tcgtgttcgt aatagtacgc taatgcctgg
    52441 agtacttgtt ttggcttttg ggtcgtcgct gcattatcca gataaactaa aggttcatca
    52501 ttgacgtctt gaaataagat aggaaaatca ttgcgtagct ttttagcatc gatcatccat
    52561 tcaacttccc ttcgattact tcgactaatt ctttccgaac ttgttctaca gggattgctg
    52621 ttaagacaga accgaggaat cctcgaatca ctaagcgctc tgcttcttct ttatgcaatc
    52681 ctctactcat cagatagtac atttcttctg gatcaactcg tcctacactg gcagcatgtc
    52741 cagcagttac ttcgttttca tcgatcaaaa gaataggatt cgcatcacca cgtgctttat
    52801 cagaaagcat caagacacga ctttcttgtt gggcatctgc accttttgcc cctttcaaga
    52861 tgtggccgat accattaaag gtcaatgttc ctttttctcg gatcactccg tgttgcaaaa
    52921 tatgcccgat cgtatgaggg gctttattgg ttactcgtgt atcgattcct tggatttgtc
    52981 tgcccgagct gattgccacg attttgactt ctgcgtgtgc accttctcct gctaaatctg
    53041 agtcaaagtc agcgatcaca tgaccgtcat tcatgacacc aattgcccag tcgacagaag
    53101 catctcgtaa aatgtgccca cggcgattca tataagaagt aatattttct cctaattgat
    53161 ctacggctga atatttgatt tttgaaccag ctttagcgat gacttccacg atgatattac
    53221 ctgatgattt tgctacttgt tctttggttg tctggaaacg ttccagataa gaaaattcac
    53281 tatgttcatc tgccacgatc aacacatgct tgaagaagtg ctcatcggat gcgccatctt
    53341 gaatgaataa ggactcgagt ggttcatcga tcacgacatt tttaggcaca tacaggaaaa
    53401 taccgctgtt cataaaagct acatggagtg cagtcaattg gtcttcgttc atgtttactg
    53461 cttttttcat ataatattct tggactagtt ctggatattc gatcattgca gtgaacaagt
    53521 cggtaaaaat gactcctttt tctgctaatt ccacaggcag ttgttcaaaa atcgtccatg
    53581 aaccttgttg gacaagcaca ggattgtctt tcattgcatc aaatgccgga atcgtgcctg
    53641 tttcactgat ggtttgtgtt tctttcacat caaataacga ccagcgatgg aatttcaccc
    53701 gatcgatatg cggcaatggc agttcatctg ctttcgctag tgcgtcttga cggaaggttc
    53761 tcatccaatc aggttcgcct ttacttaaag aaaaatcatt taccgcgtca agataatcga
    53821 gccagttttt ttctttcatt cttgctcccc cttacgcttc ttcttcttta tagtcgattc
    53881 ctaattcttt actgatacca gcgtagcctt ctgcttccag acgtttagct aggtcagcat
    53941 tccctgtcat gactactcgg ccttccatca tgatgtgaac gacatcaggt gtgatatagt
    54001 tcaataatcg ttgatagtgg gtgatgatca atgcaccgaa attgtctccg cgcatttcat
    54061 ttactccttt agcaacgact ttcaaagcat cgatatccag acctgaatcg atttcgtcta
    54121 aaattgcaaa tgtaggttct aacatcaaca attgcaggat ctcattccgt ttcttttctc
    54181 cgccagaaaa accttcgttc agataacgtt cagccatttc ttctggcata tttagtaaag
    54241 ccattttttc atctaacttt ttcaaaaatg ccataacaga aattttgtcg ttttcatcac
    54301 gtttcgcatt gatcgctgca cgcatgaatt ccgcattcgt gatacctggt atctcacttg
    54361 gatattgcat agctaaaaat aagccaagtc gcgcacgttc atctacttcc aagtctaaaa
    54421 catttttccc gtcaaacaaa atttcgcctt ctgttacttc atagtttgga ttgcccatga
    54481 tagcggcaga taaggtagat tttcctgtgc cgttaggtcc catgatggca tggatttctc
    54541 cggttttcat tgttaggttt acgcctttaa ggatttcttt ttcctcgatc gacacatgta
    54601 gattttttat ttctaagaca gacatgcatt atacccccaa attctctttc gttctcactc
    54661 attttagact aattgagaat gaagcgcaat gaccttcctt actaaaatca aatatctttt
    54721 atttattatt aaacaatttt gatttctaaa ttagaatagg tataaaaaag atgagaaata
    54781 agccattaca gacttatttc tcatcttttt tgttccatac cttattttgc atcagccttt
    54841 actggtttaa cagagccgcc ccatttgtct gtgatccatt cttggacttc tttgcggtga
    54901 agcacttcaa tcagtttttt gattttgtca ctgtcttcat cacctttacg agtcgcaata
    54961 atattcacgt aaggagagtt gtccttttca agcaatacgg catcttctaa tggattcaac
    55021 cctgcgccgt aagcgaagtt tgcgttgatt gcaactaggt caccttcatc attttggtac
    55081 gtagaagcta aaagtgatgg gtcgatcgta tggttgaact tcaagttctt agggttttct
    55141 tcaatatcat cgaatgttgc tgtttccaga tctactccat ctttgacttt gactaagcca
    55201 gcatcttgta gaattgtgat gatgcgtccc cagtctgatt cagaatttga agtaatgatt
    55261 gttgcaccat cttttaattc ggaaacatct ttgacttttt tagagtacaa gcccattggt
    55321 tcgatgtgga cagcaccggc attgacaaag tcatatcctt tttctttgat ttctttgttc
    55381 aaataaggaa tatgctggaa gtaattggca tcaatgtcgc cttcttccaa agattggttc
    55441 ggtaatacat agtcatcgaa tttgacgatt tcaagatcga ttccttcatc tttcaataat
    55501 ggtttcacat gttccaaaat ctctgcgtgc ggtgtcggtg aagcgccgac tttcaatgtc
    55561 gtgtcgtctg ctttatttgc tgaatctttg ctgtcgctgt tgttaccgca tgctgctaat
    55621 cctacagtca gcaaaagcgc tgctgcaaat ccaaaaattt tctttttcat atcctacatc
    55681 cccctatatc ctattatctt ttgtctgttt tcttagttaa gaaatcaccg attccttgaa
    55741 cgataaatac gattagtaaa ataattaaag tcgcaacgaa tgtcactgtt agattaccac
    55801 gctggtagcc ttcctgccaa gcaagacctc caagtccacc tgcgccgatc gcgccggcca
    55861 tcgcagtaaa tccgatcatc gagatcgttg taactgttaa tccagaaatc aatgcgggaa
    55921 tactttcagg tatcaagacc ttccagatga tttcccagta acttgctccc attgcatctg
    55981 ctgcttccag tacaccaggg ctgacttcac gaaaagcaat ctctactaga cgggcataaa
    56041 atggtgctgc ggataaaaca agagctggaa tggctgcttg cgcacctagc atagtgccta
    56101 cgattgcttt agtaaatgga atgatcaaaa ccatcaggat cataaatggc gttgaccgga
    56161 aaatattact gatgatcgat acgattccat aaatgattcg agcatacggt ttgtttttgc
    56221 gtccaataga gtaaaggatc aatcccaaaa ctaatccgat gatcgtcact aagaccattg
    56281 agattaatgt catatatagt gtatcaagga tagcctgatt catcgtttcc gggttgattt
    56341 gtttaaaatc tagatatgtt tctgaaaagc ttttatccac gatggatcac ctcaattcct
    56401 actccttgtt ctttgatgaa cacttctgct tgatctaatt ctttggtttc accagtcatc
    56461 aagacagtca atgaaccaaa ggaaccttct ttcacttgtt tgatctttcc atacacaacg
    56521 ttgacatcga tgttatagcg tcgaatcgct tgggaaatga ctggctcgtt ggcatttctt
    56581 tcgctaaagg tcaaagttgc caaacggccg cttggatttt ctttgatgag ttcagctaat
    56641 agttctgtcg aatcagcttc tggttcgatg tcttgttgta caaagcgttt ggttacttct
    56701 tttttcggtt gtctgaagac agtcaatacg tctccttctt ctacgacttt ccccaactcc
    56761 atcacagcga ctttgttgca gattttgcgg atcacattca tttcatgtgt gatcaagacg
    56821 atggtcaaat tcaatttctt attgatcgct aacaataagt ctaatacttc ttctgttgtt
    56881 tgcgggtcca atgcgctagt agcctcatca caaagcagga tctcaggatc atttgccaat
    56941 gctcgtgcga taccgacgcg ttgtttctgt ccgccggaaa gttgtgaagg atatgcttgt
    57001 ccgcgtcctt ctaacccgac taaacgcaac agttcttctg cccgttcttt acgtttgctc
    57061 tttggtacgc cagctaattc cagcggcagc tggatatttt caagtacagt tcttgaccat
    57121 aacaggttga aatgctggaa gatcataccg attttctttc ggaatgtacg taattctttg
    57181 ttttttaatt ttgtaatatc ttgatcgttt acgatcactt cgccgctcgt gggaagctca
    57241 agtccattca ggagacggac taatgtactt tttcctgcgc cagaatagcc gacgattcca
    57301 taaatatccc ctttttcaac gtttagtgaa acgtcattga cggcttggat gtttccgttt
    57361 ttcccattga atgtcttttt gacattcgtt agttggatta atgacatgcc attcacatct
    57421 ttctcttatg atttttcaaa caaaaaagcc ttcgccctct tgccttaagc aaaaggacga
    57481 aagcttgaac ttccgtgtta ccaccttgat ttaccgttac ttcgcaataa cagcctcttc
    57541 cagtacttgg acttagcttc tttccgtata ctgtcgcgct gtaacgggca ctcccgtagt
    57601 aagtttgctt acgctcgctt actctgctct aagaccatct tccatctcat tttcgttacc
    57661 cattttcagc aaacagggct ctcttcaaac gtccgtgaca tgtactcttc ttttcaaagc
    57721 tttctcgttt catataggat tgcttatgat tatagcaact ggaagaatca gtgtcaactt
    57781 ttttcaagtg attttctgat tttataattc gtcaaattct tttggatcaa catctttgaa
    57841 acttaagatc cacgcattca tttcatctga atcattcaag atattgatat cttcaccgat
    57901 tttatcatta acagaagaaa ctgtacccgt tagcgggctg gcaaattcat tgaccgcttt
    57961 ttccgcttca acttcgatca gaggttcatc tttttttagc gattgtcctg cttttggcag
    58021 attggcaaac gtgatgtctc caagttcttc ttgcgcttca tttgtcaaac caacacagta
    58081 ttcttttcca ttaaataaga tccaaaggtt gtcctttttc ttcaaatgct tgcctgccat
    58141 tttatatcag ttctccttca taaatttttt cattgaatcc aattgcaaca agctggtcct
    58201 ctcgtaagat cagtggtcgc ttgatcagca taccatctgt cgataagact tggctcgctt
    58261 ttgaagccgt aaactcgcct acttgatctt tcagtccaag ttcccgatat ttcatcccgc
    58321 ttgtgttgaa caggcgacgg attggtactt gactcgtttc tagccaagtc tcaatcgttg
    58381 aggccttcgg tacttctttg atcatatcaa cggtttgata actaacgcca tgctggtcta
    58441 accacgcttt ggcttttttg catgttgaac atttcgggta ccagtaaaag gtgtacaatt
    58501 ttcttacctc cttgaagatc ttgcaggtgc ttcattttgg tatctcatcg ataatatcaa
    58561 cccgataccg atcatgttcc ctaaaatcga ggatcctccc tgactaatga aaggcaatgg
    58621 gatccctgtt aaaggaagta atccgatatt tgctccaata ttctcaaaga catggaacag
    58681 cagcatcatg atcaaaccgc ttgcgatata ggcgtaaaat tcattattcg tatcgaaaca
    58741 cacccgaatc atacgataga ttaaaataaa atacaagaaa atgacgaatg caccaccgat
    58801 aaagccgaag ttttcaccga taacggtaaa aatcatatcg gactcacgaa caggcacata
    58861 gatattgctt acgttgaatc ctttaccaaa caagccaccg gtaccaattg ccaaaagtcc
    58921 ttgggccggc tgataactca ttcctgacgt atcatggaaa gggtccagcc atgaatcaat
    58981 acgtgcaaac tgataagatt tgaatccaac tttataaagc aagtctcttc cgccttcagt
    59041 cgtaaccaag aagatcgttc ctgctccaac gactactgcc aagattacta caggtgcgat
    59101 gatgcgccaa gagattccag acatcaggaa aatcccgcca aagatcgcaa gaaagacaag
    59161 catcgttcca aagtcatctt gggccatgat caatgctaaa accggtattg tcacgatcaa
    59221 gagcttccca atcagccaaa agtccgattt catcgtccgt tcttttactt gcgtattatg
    59281 tttagttacg atcagtgcca tcatcaagat gtacgcaatc ttcatcaact ctgctggctg
    59341 gaaagtaaac gcaccgaatc gaaaccagtt tttagaccct gttgaagcag ctaaattctg
    59401 atcatagaat ttgagcaaga gtcccattac cactaacccc aaaccataaa ggtaaggagt
    59461 cagtttccag atccatttgg aattcaaatg catgatgaca acgattgctg ccgctccaac
    59521 taaataccag acaacttgca tccctacgcc tttcaagact gctggattgt tcggatcatg
    59581 gctaagtgct acatataatg agagcagacc aatcaaacat aacagaaaga ctggtaaaat
    59641 cacgccataa tcaatacgat tatcgttcga agcttttaat tttttttcca tgtttcatcc
    59701 cttttcttaa aaaccatact gctgattatt atagcggtcc agttagataa tggaaagtct
    59761 ttccatagac atttgggaaa aattaacttt ccgtttgtgc agttgcttga gcaaatgcag
    59821 tacaaaagcc tactgcttat ctttagtgta tcatagaact ttttttgata catgcaatta
    59881 tctaccagct gagattctcc tcgctgtgag aagtttctgt gctttcattg tatcgaagga
    59941 tctcttcggc aatctctagc agctgatcag aaaccagatt ggttcgttgg atgaaataaa
    60001 aatatcgatg gaaattcaac ggagtcattg gcacattttc tggaacagag cgcttagaca
    60061 aaatcgagcg accgattcct tccttcagca acgcaacaat tacatcgtta cttttcacga
    60121 tcatcttctt atcttggata ttcatctctt caaaataacg ctgcgtgtaa tgaaaaacac
    60181 cagagtttgc ttctcttaca agccacaatt tgctttttgg gtccccagcg atgactagct
    60241 ggtcttccat caaagagtat ctttgtacgc cggaagtctc taatggcttt tcgataaaac
    60301 caaaatcgga ttcatgtttc gctacttgtt ctaatgattc atgagaattc gtgatttcga
    60361 tctcaaaatg cactttagga aaccttttga tcagatgttt catcaactga ggcaaaagat
    60421 agacagcaaa ggtgtgggaa gcaacgatcc gacatacttc ttcttcattt tgttcattga
    60481 gcaatttttg ctgagtttct tcccattctt ctaacaaaac cagtgtccgt tcatataata
    60541 agtcggcttg cttagttgga agcatctctt ttcttccgtt acgttgaaaa agatcgatat
    60601 tcaattcccg ctccaactgt ttgatttgat tcgagactgc tggttgagat aaaaacagta
    60661 cttctgctgc tctggaaaaa tttcttgttt catatactgc tcgaaaagtc gttaataact
    60721 taaacatggc accacttcca taacttttta tgataattac tataaaaact atttgattta
    60781 aatatacaaa aaaccatcta taatagaaat gtacaacaaa acagttgctg atccaactag
    60841 taacttagat gaagacaatc agctgtctgc ttgctcgtaa tcatacattc ctattcattt
    60901 tacaatgtat tcgttcatct tggtactaaa atctattaaa taaaaaggag aatattatat
    60961 atgaagaact ttttgaaaat tattccgatc ccgatctgcg ggttgatcct tgggttagct
    61021 tcccttggaa atctgtttaa agactatcac catgttgctt taggcaatct aactggcggt
    61081 atctctatga ttcttatgat attcattttt gttaaattgt ttatcttatt cgaacacacg
    61141 aaacaaacgt tgcatgatcc aatcatcgcc tccgtttcac caacttttac gatgtcactc
    61201 atggttattt gtacttattt tgtttcatat gaaagtattg caccaattgt caaattcatt
    61261 tggctgtgcg ctgttatctt ccaagtgatc cttgtctttt atttcaatta tcaccatgtt
    61321 atcaaagctg atctttcaat tgaagcaatt tatccaagct ggtttatcat ttacgttgga
    61381 tttggggtca tcactgtaac tgccgggaac ttcttcccac ttattggaaa aattttcttt
    61441 tggatttcat tgatctgcta tgcgttctta ttgccgatca tcatcaaacg tgtctttttc
    61501 gtaagaaata tggctcatcc gacacgtccg ctgattacga tcgttgcagc acctggttct
    61561 ctttgcctaa caggttatct taaaaacttt gctgaaccta atttttatct tgtcgtagca
    61621 ttgttcgctt tatctcaagt attgtatttc gttgtgctgt ctatgcttcc acgtttgttc
    61681 aagcttcgtt tttatccaag ctatgcagcc tttacattcc cgcttgttat ctctgcaaca
    61741 gctatattta ctactgttca ttacttcaac agtttaggta tctatagtag cgtattggat
    61801 ttcttaaaag ttgcggaatg tctgtttgca agtgtagttg tcttctacgt tcttgctcat
    61861 tacgtacgtt tcttaattaa agaacatagc aaacatggaa aatttgtttc taaaaaaatc
    61921 gcttaataat cgattcaata ggttgtgaag aagctgttct gcatcaagta ataagaacgg
    61981 ccaaacaaaa acagctgttc atgtttttgt actggttggg cttattaccg cagatgcaag
    62041 cttttgaaca ccgtttacca agaggtcgta a
//
LOCUS       DACEKH010000005        53719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEKH010000005 DACEKH010000000
VERSION     DACEKH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.005"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            160..840
                     /locus_tag="IW662_000251"
     CDS             160..840
                     /locus_tag="IW662_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0865103.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="IW662_000252"
     CDS             930..1964
                     /locus_tag="IW662_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0865104.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="IW662_000253"
     CDS             1978..2313
                     /locus_tag="IW662_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865105.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="IW662_000254"
     CDS             2297..4777
                     /locus_tag="IW662_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865106.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="IW662_000255"
     CDS             4833..6392
                     /locus_tag="IW662_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0865107.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="IW662_000256"
     CDS             6389..7753
                     /locus_tag="IW662_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0865108.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="IW662_000257"
     CDS             7924..8994
                     /locus_tag="IW662_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0865109.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="IW662_000258"
     CDS             9009..9677
                     /locus_tag="IW662_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865110.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="IW662_000259"
     CDS             9697..10275
                     /locus_tag="IW662_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0865111.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="IW662_000260"
     CDS             10676..11587
                     /locus_tag="IW662_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0865112.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSEKKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="IW662_000261"
     CDS             complement(11965..12882)
                     /locus_tag="IW662_000261"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ0865113.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="IW662_000262"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="IW662_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ0865114.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="IW662_000263"
     CDS             13579..14127
                     /locus_tag="IW662_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ0865115.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="IW662_000264"
     CDS             14242..16602
                     /locus_tag="IW662_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0865116.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="IW662_000265"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="IW662_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0865117.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="IW662_000266"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="IW662_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ0865118.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="IW662_000267"
     CDS             19108..20007
                     /locus_tag="IW662_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865119.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="IW662_000268"
     CDS             20000..21262
                     /locus_tag="IW662_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0865120.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="IW662_000269"
     CDS             21423..23321
                     /locus_tag="IW662_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ0865121.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="IW662_000270"
     CDS             complement(23651..24370)
                     /locus_tag="IW662_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ0865122.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24707..25039
                     /locus_tag="IW662_000271"
     CDS             24707..25039
                     /locus_tag="IW662_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865123.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="IW662_000272"
     CDS             25026..27029
                     /locus_tag="IW662_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ0865124.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="IW662_000273"
     CDS             27048..27518
                     /locus_tag="IW662_000273"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ0865125.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="IW662_000274"
     CDS             27539..28123
                     /locus_tag="IW662_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ0865126.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="IW662_000275"
     CDS             28138..29124
                     /locus_tag="IW662_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ0865127.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="IW662_000276"
     CDS             29114..29425
                     /locus_tag="IW662_000276"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ0865128.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="IW662_000277"
     CDS             29447..31228
                     /locus_tag="IW662_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ0865129.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="IW662_000278"
     CDS             31221..32597
                     /locus_tag="IW662_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ0865130.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="IW662_000279"
     CDS             32604..33239
                     /locus_tag="IW662_000279"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ0865131.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="IW662_000280"
     CDS             33269..34624
                     /locus_tag="IW662_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ0865132.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="IW662_000281"
     CDS             complement(34816..36210)
                     /locus_tag="IW662_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0865133.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="IW662_000282"
     CDS             36302..37273
                     /locus_tag="IW662_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0865134.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="IW662_000283"
     CDS             37270..38052
                     /locus_tag="IW662_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ0865135.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="IW662_000284"
     CDS             complement(38221..38592)
                     /locus_tag="IW662_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0865136.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="IW662_000285"
     CDS             38726..39169
                     /locus_tag="IW662_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0865137.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="IW662_000286"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="IW662_000286"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ0865138.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="IW662_000287"
     CDS             complement(40040..41407)
                     /locus_tag="IW662_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0865139.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="IW662_000288"
     CDS             complement(41391..42221)
                     /locus_tag="IW662_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ0865140.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="IW662_000289"
     CDS             42384..42821
                     /locus_tag="IW662_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ0865141.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /locus_tag="IW662_000290"
     CDS             42897..43517
                     /locus_tag="IW662_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ0865142.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43706..44212
                     /locus_tag="IW662_000291"
     CDS             43706..44212
                     /locus_tag="IW662_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ0865143.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44229..45128
                     /locus_tag="IW662_000292"
     CDS             44229..45128
                     /locus_tag="IW662_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ0865144.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45215..46099
                     /locus_tag="IW662_000293"
     CDS             45215..46099
                     /locus_tag="IW662_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0865145.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46291..46362)
                     /locus_tag="IW662_000294"
     tRNA            complement(46291..46362)
                     /locus_tag="IW662_000294"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46327..46329),aa:Arg,seq:ccg)
     gene            46422..47756
                     /gene="rpoN"
                     /locus_tag="IW662_000295"
     CDS             46422..47756
                     /gene="rpoN"
                     /locus_tag="IW662_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ0865146.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47959..48996
                     /locus_tag="IW662_000296"
     CDS             47959..48996
                     /locus_tag="IW662_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ0865147.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            49037..50038
                     /gene="gap"
                     /locus_tag="IW662_000297"
     CDS             49037..50038
                     /gene="gap"
                     /locus_tag="IW662_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0865148.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            50148..51338
                     /locus_tag="IW662_000298"
     CDS             50148..51338
                     /locus_tag="IW662_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ0865149.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51442..52197
                     /locus_tag="IW662_000299"
     CDS             51442..52197
                     /locus_tag="IW662_000299"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ0865150.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52313..53611
                     /gene="eno"
                     /locus_tag="IW662_000300"
     CDS             52313..53611
                     /gene="eno"
                     /locus_tag="IW662_000300"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ0865151.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        17827 a         9355 c        11351 g        15186 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaaaaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgactc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacgattttt gtcacacctt cttttttgta tataaaaatc
    43621 ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg tacgtcatat
    43681 taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa aacggcagat
    43741 cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa aaatgttccg
    43801 tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc aagatagttt
    43861 tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt
    43921 ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa ggacgcctgt
    43981 ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt ccgttgcaga
    44041 gctccatatg attttagata aaatagacag tccggaaaaa gagattctga tcctctactt
    44101 ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg aaaacacagt
    44161 taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat gaaggagaga
    44221 agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact tataaagaac
    44281 aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc gaagagcaat
    44341 catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta tctttggcta
    44401 tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc tttgcagaag
    44461 atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt actgatcgat
    44521 ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa
    44581 caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt gaaaaagctg
    44641 aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat tatcagaaaa
    44701 tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca gcagcagata
    44761 gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag ctctcacttc
    44821 cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc aaacggcaga
    44881 tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa gatattcaag
    44941 aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat ttgaacaaag
    45001 agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat atgggtacac
    45061 cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa gattatttgg
    45121 atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa
    45181 taataagaag agaaaaagag aaagaagaga tatagtgaaa agaaattatt tagttatcag
    45241 tattattgct ttatccgtgc ttttgttttt cgaaattact tcggtggcct ccttgatcac
    45301 cgaagtaaaa ggacaaacac actcttcgga gcattcgtca caatctttca aacaatcaga
    45361 acaatcaacc attaaaagcc aatcaactga aacagctaac gtcaaagaag ccattcaaga
    45421 atcagcggaa aaggtacaaa gtcagtcatc acaatcaaca caagacagta caaaaacgag
    45481 ccgagatccg gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag gagcgaccac
    45541 aacaccgcag cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt
    45601 aggagaaaat acggcgcagc atcctgaaat cgtgaagcag acagctgaag cagggcacga
    45661 gattggcaat cacacatacg atcatcagaa tctggccact ctttctgccc agtcgatgac
    45721 ggaggaagtg acgaaagcag acactgagat caaaaaagtc actggcaaaa cccccacttt
    45781 tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat
    45841 catcgaatgg tctgttgatt cagaagattg gaagacaaga aatcctgatt tgattttgca
    45901 gaagatccaa gcaacagtat acgatggggc aattatttta ttccatgata tctatcctga
    45961 aacgattcgt gccgttcctc aagtgatcga ttatctgaaa gagcaagggt atcgcatcac
    46021 gacagttggg gatttattag ggcatccaac ggctgtcgag aactattatg gcagaaacga
    46081 tcatcggcca gttcagtaag tgagacaagt atctttaata aataacacat tttaataagt
    46141 agttgaacac ataactacct tacttgcaaa ggcttaacaa tatctataca ggtacaacaa
    46201 aaaactgtcc accaattttc tttggcggac agttttcact taaaaataaa gaagcaacta
    46261 ttcgtaaatt atgctaaaat agcagtagaa cgcgtcctga gggaatcgaa cccccgtctc
    46321 aagaaccgga atcttacgtg atatccacta cactaaggac gcaagcactg ggataattgt
    46381 aactaaacag ataaaaaatt acaaggggat tggacaaaaa aatgaaattt gagcaaaatt
    46441 tttcacaaaa acagcagcag acacagaaac tagccatgac gcaacagttg caacagtcca
    46501 tccaagtttt acaatataac agtgaggagc tgttggcatt cgttgagaat caagcaatgg
    46561 aaaatccatt ggtggaagtt gttgagccgg aatggcagcc agattacata aaagcttctt
    46621 catcttctta tgaaggagaa gagacaaatt acctaaatca gattcctgat acaaaagggt
    46681 ctttatttga ttcattgatc gaacaagtcc atttgaatta ccgagatact tttttgagga
    46741 aaatcgttct ttatcttgta gaatacatcg atttgaacgg atttttgacg attagtttag
    46801 aagaagccgg aaaagagatt ggagctacgc ctatccaaat gctcgatgcg ttgactctga
    46861 ttcagcaatt agaaccagct ggagtaggtg cacgtagttt gcaagaatgt ctcatgcttc
    46921 aaacagaacg agatgattat gcgccggaat tagcttatat cgtgttggaa gaatgttttg
    46981 aggaactggt ggagcgaaaa tggaaagaaa tcgcacaacg tttttctgtt gatctacatg
    47041 ctgttcagca gatattcgac tatttacaaa cgttgagtcc aagccctggc cggattttcg
    47101 accgtagcag cgaattgtac attattcctg atgtccgggt attagtggac gacgataaaa
    47161 atgtacaagt tatctcaaac cgaaaaaatc agccaaatat acgctttcaa gagagttact
    47221 tcaaacaaat gtcacaacaa gcagataaag aaactgaaaa ttatttaaaa gaacggaaac
    47281 aggaattcga atggttgaag aaaacgatcc tacaacgtgg ggatacaatt ttgcgggttg
    47341 ctcaagtcat tgtttcccgg caaaaagctt tctttatcga caaggaacgt ccaatcaagc
    47401 ctcttacgtt aaaggaagtt gcaactgaga tagatgtgca tgaatccacg gtcagtcgtg
    47461 cagtcaacgg gaaatatttg gaaacagact tcggtgtatt tgaattaaaa aaatttttca
    47521 ctacacgaat tcctacgaat agtacagaac agacagaaga cctttcagca gatacagcga
    47581 agaaaaagct gcaggaatta gtcgatcaag aagacaaaaa caagccgttg tctgaccaga
    47641 aattggttga attgctgaaa aaagatgaga tagcgatctc aagacgaaca gtagcaaaat
    47701 atcgtgattt attaggaatc ccgagttcat caaaaagaaa acgttatgat aactaatcag
    47761 tcaaaagact agaaaaagta tttcactttt tctagtcttt ttagttgaaa aacgagagca
    47821 aaactgttag acttacagtc gtgaggttga gatttccctt tattgatgtg aaattcgaat
    47881 agttaatttc tttttttatc gtatatgggt cgttttaagt ctacattgga cgtaaagtgt
    47941 ccaactaagg agatcgctat gctggatgaa ataaaaatga ttgaatctgt tgcaccagat
    48001 attatcgaag tgttgcaaga acgctataaa attttacgaa atatctattg gatgcagccg
    48061 attggacgtc gaagtttatc ggaaagtatg gggatcacag aacgagtatt gcgaacagaa
    48121 acagatcttt tgaaaaatct gaatttgatc gacacctcaa aaagcggtat gacgctgaca
    48181 actaagggcg aagaagttta tcaaagcctt gaaaacttca tggatcagct tttaggtacg
    48241 catcaaaccg aacaacagtt ggcagaatat tttggtaacc aacgctgtat cgttgtttca
    48301 ggaaacagcg aagagcagac caaagtggct gatgcattcg gcgaagcgct gagcgaagcc
    48361 ctagaccgac tacttccaga aggcgagaat atcatcgctg tgatgggagg aactactatg
    48421 gctgtggtag ccgaacaatt gtctaatctg gaaaataaga aacggcataa cctgtttgtc
    48481 cctgcaagag gcgggattgg tgaagcgata actgtacagg caaattctgt cagtgcaaga
    48541 atggctgcta aagcaaacgg taaccaccgt gcactttatg ttccggaaca attaagtctg
    48601 gcaacttaca attctttact caatgagcca tctatccaag aagtcttaaa cttgattagt
    48661 gaagcgaatt gtgttattca tagtattggg cgtgcattac acatggcagc acgtcgtaag
    48721 atgactgaaa aagaattagt catgctgaaa caagcaaatg ctgttgcgga atcttttggc
    48781 tactttttca atgaaaaagg tgaagtcgtt tataaggtcc ctagaatcgg catagaattg
    48841 caagatttgg aaaaagtccc gatcatcatg gcaatcgcgg gcggaaaatc aaaagctaaa
    48901 gcaattcgag cttatatgaa aaatgcacca aaacaaacgt ggctcatcac tgatgaagct
    48961 gctgcaaatg agattttaaa aggggtaacc ctttaaaata aatatttttt tgattttcat
    49021 aaggaggaaa tcttaaatga cagttaaagt aggtattaat ggttttggac gtattggacg
    49081 tctagcattc cgtcgtatcc aagatgtaga tggaatcgaa gtagtagcaa tcaacgactt
    49141 gacagatgct aaaatgttgg cacacttgtt aaaatacgac acaactcaag gacgtttcaa
    49201 cggaacagtt gaagttcatg aaggttcatt caacgtaaac ggaaaagaag ttaaagtatt
    49261 agctaaccgc aacccagaag aattaccatg gggcgaacta ggcgtagata tcgttctaga
    49321 atgtactggt ttcttcactt ctaaatcagc agctgaaaaa catttaacag ctggtgctaa
    49381 acgcgttgtt atctctgctc ctggcggaaa cgatgttcca acaatcgttt ataacacaaa
    49441 ccacgaaaca ttaactggta aagaaacagt tatctcaggt gcttcatgta ctactaactg
    49501 tttagctcct atggctaaaa cattgaacga caaatttggt gttgttgaag gattaatgac
    49561 aactatccac gcttacacag gtgaccaaat gactctagac ggacctcatc ctaaaggtga
    49621 cttccgccgt gcacgcgctg ctgctgcaaa catcgttcct aactcaacag gtgctgctaa
    49681 agctatcggt ttagtaattc ctgaattgaa cggtaaatta gacggagctg ctcaacgtgt
    49741 tcctgtacca acaggttcat taacagaatt agtaacagtt cttgaaaaag aagtaactgt
    49801 tgacgaaatc aatgcagcaa tgaaagaagc ttcaaacgaa tcttacggat acaacacaga
    49861 cgaaatcgtt tcttcagata tcgttggtat gacttacggt tcattatttg atgctacaca
    49921 aactaaagta atgacagttg gcgacaaaca attagttaaa actgttgctt ggtacgacaa
    49981 cgaaatgtca tacactgcac aattagtacg tactttagaa tacttcgcta acttataaga
    50041 tagacaaacg tacaagtgta aattatagac atgtacattt aaaagcgggg aagcaatcgc
    50101 gcttcttccg ctttttttga taataagttt attcaggagg aaataaaatg gctaagaaaa
    50161 cagtgaaaga tatcgattta aaagacaaaa aagttcttgt ccgtgttgac ttcaacgttc
    50221 ctttgaaaga cggcgtgatc actgacgaca ctcgtatcaa agcagcattg ccaacaatca
    50281 actacgttct agaacaaggc ggaaaagcaa tccttttctc tcaccttgga cgtgtaaaaa
    50341 cagaagaaga taaagaagga aaatctttag ctccagttgc aaaacgtcta ggcgaattat
    50401 taggtaaaga agtaacattc gtacctgaaa ctcgcggaga acaattagaa gaagcaatcc
    50461 gcaacatgaa tgacggcgac gttgtcgtat tcgaaaatac acgatttgaa gatattgacg
    50521 gtaaaaaaga aagcaaaaat gatacagaac taggtaaata ctgggcttca ttaggcgatg
    50581 tatttgttaa cgatgctttc ggtacagctc accgtgcaca tgcttctaac gtaggtatcg
    50641 cttcaactgg tatcccaaca gttgctggat tcttgatgga aaaagaaatc aagttcatcg
    50701 gtgaagcagt agaagaacca aaacgaccaa tgattgcaat ccttggtggc gcaaaagttt
    50761 ctgataaaat cggcgtaatc gaaaacttga ttcctaaagc agataaaatc ttgatcggtg
    50821 gcggaatgac ttacacattc tacgaagcaa aaggaatcaa gatcggtaac tctctagtag
    50881 aagctgataa agtggaacta gcaaaagaat taatcgaaaa agcaggcgac aaacttgtct
    50941 tgccaatcga caatgtctgt gcaccagaat tttcaaatga tgttgaaaca caagtaatcg
    51001 aaggagacat tcctgatggt ttgatggctt tagacatcgg cccagcatca gtaaaattat
    51061 ttgctgatac attacaaggt gcaaaaacag ttgtttggaa cggacctatg ggtgtatttg
    51121 aaatgagtaa ctttgctaaa ggtacaatcg gtgtttgtga agcaatcgct aacttagaag
    51181 gcgcaacaac aatcatcggt ggcggagact cagctgcagc agcagaacaa ttaggatttg
    51241 ctgacaaatt cacacacatc tcaacaggtg gtggcgcaag cttggaattg cttgaaggta
    51301 aagaattacc aggactagca gcaatcaacg ataaataaac gaccaagttt gctcaattct
    51361 gacgagtaat cgatcaaaag gtgaagggag ttttcatgac tccctagcca tttttcaata
    51421 aattaaggag tgtgttcctc aatgcgtaaa cctattatcg caggtaactg gaaaatgaac
    51481 aaaactgcat cagaagcaaa agaatttgct gaagcagtaa aaaacaaaat cccttcaaat
    51541 gacaaagttg attcagtcat cggatctcct gcattattct tacaagaatt aatgacatca
    51601 gctgaaggta cagaactaaa aatctcagca caaaactgct actgggaaaa ctcaggtgcg
    51661 ttcactggtg aaacttcacc agcagctctt gctgatctag gtgttcatta tgtgatcatc
    51721 ggacattcag aacgtcgtga atatttccat gaaacagacg aagacatcaa taaaaaagca
    51781 aaagctatct ttgcaaatgg tatgacacca atcctatgct gcggcgaatc tttagaaacc
    51841 tatgaagcag gcaaaactgc tgaatggatc gaaggacaaa ttacagctgg tttagctgat
    51901 ctttcagctg aacaagtgtc aaacatggtt attgcttatg aaccaatctg ggcaatcggt
    51961 actggtaaat cagctgacgc acaaatcgct gacgaaattt gtggagttgt acgtcaaaca
    52021 gttgaaaaac tttatggaaa agaagtatca gaagcagttc gtatccaata cggcggttct
    52081 gtaaaaccag aaaatattgc tgaatacatg gcaaaagaaa atgttgacgg tgctttagtc
    52141 ggtggcgcaa gcttgcaagc agattcattt ttgtcattgt tagacgctgt aaaataattg
    52201 ttgaaaattt gcgaaaaatg caagaaatgt ttgcactaat gagcaaaatt aactaaaatc
    52261 gtatatgagg gaaatccctt aggaaaaaac aaactcaaag gagagacaaa acatgtcaat
    52321 tattactgat gtttacgctc gcgagatctt agactcacgt ggtaacccaa caatcgaagt
    52381 agaagtatac acagaaagcg gagcttttgg ccgcggaatg gttccttcag gtgcttcaac
    52441 tggtgaatac gaagcagttg aattacgtga cggagacaaa gctcgttatg gcggtaaagg
    52501 tgtaactaaa gctgtcgaca acgtaaacaa catcattgct gaagcaatca ttggctacga
    52561 tgttcgtgac caaatggcta tcgacaaagc tatgatcgct ttagacggaa ctcctaacaa
    52621 aggtaaatta ggtgcgaacg ctattttagg tgtttctatc gctgttgcac gtgcagctgc
    52681 tgactaccta gaagtacctt tataccacta cctaggcgga ttcaacacaa aagtattgcc
    52741 aacaccaatg atgaacatca tcaacggcgg atcacatgct gataactcaa tcgacttcca
    52801 agaattcatg atcatgcctg ttggcgctcc aacattcaaa gaagctctac gtatgggtgc
    52861 tgaagtattc cacgcattag cttcaatctt gaaagctcgc ggattagcaa cttctgttgg
    52921 tgacgaaggt ggatttgctc caaaccttgg ttcaaacgaa gaaggttttg aagtaatcat
    52981 cgaagcaatc gaaaaagctg gctatgtacc tggtaaagac gttgttcttg ctatggatgc
    53041 tgcttcttca gaattctacg acaaagaaaa aggtgtttac gtactagctg actcaggcga
    53101 aggcgaaaaa acaactgacg aaatgatcaa attctacgaa gaattagttt ctaaatatcc
    53161 aatcatctca atcgaagatg gattagacga aaacgactgg gacggattca agaaattaac
    53221 tgacgtatta ggcgacaaag ttcaattagt tggtgacgac ttgttcgtaa caaacactca
    53281 aaaattatct gaaggtatcg aaaaaggaat tgctaactca atccttatca aagtgaacca
    53341 aatcggtaca ttaacagaaa ctttcgaagc tatcgaaatg gctaaagaag ctggctacac
    53401 agcagttgta tctcaccgtt ctggtgaaac agaagattca acaatctctg atatcgctgt
    53461 tgcaacaaat gctggccaaa tcaaaactgg ttctctatca cgtactgacc gtattgctaa
    53521 atacaaccaa ttactacgta tcgaagacca acttggtgaa gttgcagaat acaaaggttt
    53581 gaaatctttc tacaacttaa aaaacaaata attcttttct aatgaagata catgatcgac
    53641 tattgaaaga tagtcgagta tgatcactta gttaagtaat cgaactctct acttgctgaa
    53701 atatcagcag gtagagagt
//
LOCUS       DACEKH010000006        53490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEKH010000006 DACEKH010000000
VERSION     DACEKH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.006"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..340
                     /locus_tag="IW662_000301"
     CDS             <1..340
                     /locus_tag="IW662_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ0865152.1"
                     /translation="LVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELSRKSVQTL
                     FKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWILLKQGGL
                     ALLALLSIFL"
     gene            463..2094
                     /locus_tag="IW662_000302"
     CDS             463..2094
                     /locus_tag="IW662_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ0865153.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(2156..2797)
                     /locus_tag="IW662_000303"
     CDS             complement(2156..2797)
                     /locus_tag="IW662_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0865154.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            3079..3387
                     /gene="rplU"
                     /locus_tag="IW662_000304"
     CDS             3079..3387
                     /gene="rplU"
                     /locus_tag="IW662_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ0865155.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            3414..3758
                     /locus_tag="IW662_000305"
     CDS             3414..3758
                     /locus_tag="IW662_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ0865156.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3771..4064
                     /gene="rpmA"
                     /locus_tag="IW662_000306"
     CDS             3771..4064
                     /gene="rpmA"
                     /locus_tag="IW662_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ0865157.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4471..7305
                     /locus_tag="IW662_000307"
     CDS             4471..7305
                     /locus_tag="IW662_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0865158.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            7496..7987
                     /locus_tag="IW662_000308"
     CDS             7496..7987
                     /locus_tag="IW662_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ0865159.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            8090..9079
                     /gene="manX"
                     /locus_tag="IW662_000309"
     CDS             8090..9079
                     /gene="manX"
                     /locus_tag="IW662_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ0865160.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            9112..9915
                     /locus_tag="IW662_000310"
     CDS             9112..9915
                     /locus_tag="IW662_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ0865161.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9936..10850
                     /locus_tag="IW662_000311"
     CDS             9936..10850
                     /locus_tag="IW662_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ0865162.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10933..11307
                     /locus_tag="IW662_000312"
     CDS             10933..11307
                     /locus_tag="IW662_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ0865163.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11446..11691
                     /locus_tag="IW662_000313"
     CDS             11446..11691
                     /locus_tag="IW662_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0865164.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11863..12927
                     /locus_tag="IW662_000314"
     CDS             11863..12927
                     /locus_tag="IW662_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0865165.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            13173..13598
                     /locus_tag="IW662_000315"
     CDS             13173..13598
                     /locus_tag="IW662_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0865166.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13591..14040
                     /gene="nusB"
                     /locus_tag="IW662_000316"
     CDS             13591..14040
                     /gene="nusB"
                     /locus_tag="IW662_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ0865167.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            14137..14991
                     /locus_tag="IW662_000317"
     CDS             14137..14991
                     /locus_tag="IW662_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ0865168.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14992..16329
                     /locus_tag="IW662_000318"
     CDS             14992..16329
                     /locus_tag="IW662_000318"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ0865169.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            16334..16558
                     /locus_tag="IW662_000319"
     CDS             16334..16558
                     /locus_tag="IW662_000319"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ0865170.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16560..17450
                     /locus_tag="IW662_000320"
     CDS             16560..17450
                     /locus_tag="IW662_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0865171.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17459..18277
                     /locus_tag="IW662_000321"
     CDS             17459..18277
                     /locus_tag="IW662_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ0865172.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            18384..18833
                     /locus_tag="IW662_000322"
     CDS             18384..18833
                     /locus_tag="IW662_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0865173.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18851..20533
                     /gene="recN"
                     /locus_tag="IW662_000323"
     CDS             18851..20533
                     /gene="recN"
                     /locus_tag="IW662_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ0865174.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20588..20704)
                     /locus_tag="IW662_000324"
     CDS             complement(20588..20704)
                     /locus_tag="IW662_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ0865175.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20777..21733)
                     /locus_tag="IW662_000325"
     CDS             complement(20777..21733)
                     /locus_tag="IW662_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0865176.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21904..22272
                     /locus_tag="IW662_000326"
     CDS             21904..22272
                     /locus_tag="IW662_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ0865177.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22442..22873
                     /gene="mraZ"
                     /locus_tag="IW662_000327"
     CDS             22442..22873
                     /gene="mraZ"
                     /locus_tag="IW662_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ0865178.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22894..23853
                     /gene="rsmH"
                     /locus_tag="IW662_000328"
     CDS             22894..23853
                     /gene="rsmH"
                     /locus_tag="IW662_000328"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ0865179.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23858..24259
                     /gene="ftsL"
                     /locus_tag="IW662_000329"
     CDS             23858..24259
                     /gene="ftsL"
                     /locus_tag="IW662_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0865180.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            24260..26452
                     /locus_tag="IW662_000330"
     CDS             24260..26452
                     /locus_tag="IW662_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0865181.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26478..27440
                     /locus_tag="IW662_000331"
     CDS             26478..27440
                     /locus_tag="IW662_000331"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ0865182.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27454..28815
                     /locus_tag="IW662_000332"
     CDS             27454..28815
                     /locus_tag="IW662_000332"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ0865183.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28841..29929
                     /gene="murG"
                     /locus_tag="IW662_000333"
     CDS             28841..29929
                     /gene="murG"
                     /locus_tag="IW662_000333"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0865184.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29944..31164
                     /locus_tag="IW662_000334"
     CDS             29944..31164
                     /locus_tag="IW662_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ0865185.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            31320..32645
                     /gene="ftsA"
                     /locus_tag="IW662_000335"
     CDS             31320..32645
                     /gene="ftsA"
                     /locus_tag="IW662_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ0865186.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32668..33909
                     /gene="ftsZ"
                     /locus_tag="IW662_000336"
     CDS             32668..33909
                     /gene="ftsZ"
                     /locus_tag="IW662_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ0865187.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33926..34603
                     /locus_tag="IW662_000337"
     CDS             33926..34603
                     /locus_tag="IW662_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ0865188.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34617..35216
                     /locus_tag="IW662_000338"
     CDS             34617..35216
                     /locus_tag="IW662_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ0865189.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            35231..35518
                     /locus_tag="IW662_000339"
     CDS             35231..35518
                     /locus_tag="IW662_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ0865190.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35567..36349
                     /locus_tag="IW662_000340"
     CDS             35567..36349
                     /locus_tag="IW662_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ0865191.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            36370..37077
                     /locus_tag="IW662_000341"
     CDS             36370..37077
                     /locus_tag="IW662_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ0865192.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            37339..40137
                     /gene="ileS"
                     /locus_tag="IW662_000342"
     CDS             37339..40137
                     /gene="ileS"
                     /locus_tag="IW662_000342"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ0865193.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(40186..41712)
                     /locus_tag="IW662_000343"
     CDS             complement(40186..41712)
                     /locus_tag="IW662_000343"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ0865194.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41727..42374)
                     /locus_tag="IW662_000344"
     CDS             complement(41727..42374)
                     /locus_tag="IW662_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ0865195.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42558..42887)
                     /locus_tag="IW662_000345"
     CDS             complement(42558..42887)
                     /locus_tag="IW662_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ0865196.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            43064..43792
                     /gene="sfsA"
                     /locus_tag="IW662_000346"
     CDS             43064..43792
                     /gene="sfsA"
                     /locus_tag="IW662_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ0865197.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43808..44821
                     /locus_tag="IW662_000347"
     CDS             43808..44821
                     /locus_tag="IW662_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0865198.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALIDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44821..46098
                     /locus_tag="IW662_000348"
     CDS             44821..46098
                     /locus_tag="IW662_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0865199.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            46161..48863
                     /locus_tag="IW662_000349"
     CDS             46161..48863
                     /locus_tag="IW662_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0865200.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            49015..49332
                     /locus_tag="IW662_000350"
     CDS             49015..49332
                     /locus_tag="IW662_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0865201.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            49362..49682
                     /locus_tag="IW662_000351"
     CDS             49362..49682
                     /locus_tag="IW662_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0865202.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49790..51250
                     /locus_tag="IW662_000352"
     CDS             49790..51250
                     /locus_tag="IW662_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0865203.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            51318..51539
                     /locus_tag="IW662_000353"
     CDS             51318..51539
                     /locus_tag="IW662_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865204.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51570..51752
                     /locus_tag="IW662_000354"
     CDS             51570..51752
                     /locus_tag="IW662_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ0865205.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51752..52165
                     /locus_tag="IW662_000355"
     CDS             51752..52165
                     /locus_tag="IW662_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ0865206.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            52288..53469
                     /locus_tag="IW662_000356"
     CDS             52288..53469
                     /locus_tag="IW662_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0865207.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
BASE COUNT        17702 a         9060 c        11587 g        15141 t
ORIGIN      
        1 tttagtgact ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac
       61 tgattctgac acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc
      121 tgttcaaaca ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact
      181 ttatggagct tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat
      241 tttttttgtt gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg
      301 tgggctagct ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt
      361 tatctaacag gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat
      421 gaatggtatt ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt
      481 tcctcactaa aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg
      541 gctgggatca cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc
      601 ggagctgatg cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt
      661 ttatcaggcg gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg
      721 tctattgcgg caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta
      781 tttttacttg cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc
      841 gtaattacgg ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat
      901 ttatttggtg ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg
      961 ataggatttc cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg
     1021 tttatctatc caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg
     1081 gcaacagcag ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa
     1141 acattgatca gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa
     1201 gtggcagttc cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt
     1261 gcttcggctg gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa
     1321 ggaattggaa atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc
     1381 cgtacaagcg tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca
     1441 gttgtcttac tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca
     1501 gcacttgcag gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag
     1561 gagctattta ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt
     1621 acagttatct ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt
     1681 tttgttgtca aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg
     1741 gggctgccag aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg
     1801 ttttttatgt cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt
     1861 gtcattttct ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat
     1921 gaatttcaag aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca
     1981 gaagtagaaa aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca
     2041 gttgctcatg cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat
     2101 gaaaaagaac agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat
     2161 attgctgtaa aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc
     2221 gattgtagaa aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc
     2281 attcgtaggc atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat
     2341 gatttttggt aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg
     2401 tcgcaattaa ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa
     2461 atacttcgga acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt
     2521 tattgatccg atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct
     2581 cttctcgtgt taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc
     2641 taaaaggaac atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt
     2701 taggatgaag aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta
     2761 aagaagcttc ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta
     2821 ttataagaat aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa
     2881 aagcagaagt attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac
     2941 ggttgctgtt ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc
     3001 agaacaagta ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag
     3061 aaggaggtgc tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt
     3121 gaagtgggac aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt
     3181 tttgacgaag ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca
     3241 ggtgcaactg ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc
     3301 aaatacaaac ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa
     3361 gtggttatcg acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca
     3421 aaggaacatt taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg
     3481 ctgatgctgg tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca
     3541 gcactgtcaa cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag
     3601 aagaaggcgg ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata
     3661 ttgctgagat tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact
     3721 cagaatatat ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat
     3781 ctatgaatct acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg
     3841 actcagaatc aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat
     3901 caattcttta ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg
     3961 gagacgacac tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta
     4021 acaaaaaaca agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta
     4081 aagaggttgt gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag
     4141 tggaggctac gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat
     4201 caggatgttt tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg
     4261 aaataattat atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg
     4321 atttttcgtt cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa
     4381 tcaggtaaat agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt
     4441 acaatagtct atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg
     4501 tatgtcaaag aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca
     4561 caggaaattg ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag
     4621 ctagtgcgcg aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa
     4681 acactcgtta cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg
     4741 gtctctgaag aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt
     4801 ggagctaacg gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg
     4861 ccaagaggat taaattgttt gattacagga cctaccggat caggaaagac ttattttgca
     4921 cacgcgatgt atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt
     4981 gttttcaatt gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg
     5041 tacgcaaaag gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg
     5101 gatggcggta tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg
     5161 atcttttact ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat
     5221 gaagcaaatg tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa
     5281 acttttgttc ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca
     5341 aatgagaaag tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga
     5401 aagatttctt tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat
     5461 ataggacaat tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg
     5521 caatcacccg aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg
     5581 attcaattag caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag
     5641 ataactgtag caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct
     5701 tataatctat atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat
     5761 caagaagcaa tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat
     5821 aaagaccatg gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa
     5881 atcatagaag tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt
     5941 cagcaaaact ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac
     6001 ttaggagaag aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat
     6061 gaatatgctg ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt
     6121 ccagaaagtg aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca
     6181 agcggcagaa tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc
     6241 caagttgtcc aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat
     6301 atggatccga aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga
     6361 agcggggtca tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa
     6421 agagacactg gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa
     6481 actgtacgaa aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa
     6541 aattttcgag gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt
     6601 cataagaaaa aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc
     6661 aaagaattga tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct
     6721 ttgtcgattg tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata
     6781 gctactactg ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt
     6841 attgatcaaa atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa
     6901 actgaattca tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct
     6961 gataacttca tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca
     7021 caaatctctc agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg
     7081 atgcacacag caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa
     7141 aacgaattga caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt
     7201 ctgttggaag atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt
     7261 ctagtccata atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat
     7321 acacttttat agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg
     7381 tatcgtaaaa aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt
     7441 cacacagaaa tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga
     7501 tattcgattg gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc
     7561 gaagcaaaca ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt
     7621 gagaaagttt ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt
     7681 gcagaaaatg attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt
     7741 attcaccaat ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt
     7801 gaatatcggc ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt
     7861 aaatcaacaa gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat
     7921 tcgaaaagta cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa
     7981 ggtgtagcaa gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt
     8041 attaatattt ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat
     8101 tatcctagca agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt
     8161 tggcgaacaa gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt
     8221 gagagccaaa ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt
     8281 agttgattta tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa
     8341 agataaatgg gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc
     8401 acgcttttca atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga
     8461 cggtgtaaaa gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga
     8521 agacgcacaa cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa
     8581 atatgtatta gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac
     8641 aaaagcagtg caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct
     8701 gcgtaagaga ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat
     8761 tagcaaaatg atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt
     8821 atttgaaaat cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt
     8881 aaatgttggg tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat
     8941 gggacaagaa gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt
     9001 acgtaaagta cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc
     9061 tgaattagca aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt
     9121 atttcaataa ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat
     9181 gaatttcaat ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac
     9241 ctcgaagctg gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat
     9301 atcggtgccg ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta
     9361 gttttaggtg gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg
     9421 gctgttgcag ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat
     9481 ttgatggatg cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt
     9541 gctgtatgtt tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca
     9601 gcacaaactg tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct
     9661 atcggtgggg gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca
     9721 aaagaagtat ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca
     9781 ttgattgctc taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa
     9841 atgggtggtt catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt
     9901 ttgaatgact attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat
     9961 caaaaaaaga tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact
    10021 acgaacgtat gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat
    10081 acaaaacaaa agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac
    10141 acccatacat tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga
    10201 atggcgcgcc tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt
    10261 tagctggtgt tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag
    10321 gtgcttcatt agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga
    10381 acattattcg ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta
    10441 aaattactga tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt
    10501 tagggatgtt cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag
    10561 ttgtatcaac tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag
    10621 gtggtgaagg aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg
    10681 aaaaggtaac aacattacaa gacaacttag accaattgat tcctggttta gctgcattag
    10741 gattaacatt gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc
    10801 ttggtttatt cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa
    10861 tatagctcgg gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc
    10921 tggagggaga aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact
    10981 tcgtttacgg gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt
    11041 taccatgcgc gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta
    11101 ctagcatcag tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa
    11161 aatggtacat acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa
    11221 tatgtagatg aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt
    11281 attcgtagta tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct
    11341 ttattttttt actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt
    11401 aaatcccata tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg
    11461 acattgattg ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat
    11521 tcgatgggct gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg
    11581 attttcggcg aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc
    11641 gcagtgattt tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca
    11701 aaatagtaaa ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt
    11761 aaaataaaaa gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg
    11821 ctataatgga aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa
    11881 aaattgcgca aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac
    11941 aatcttcgtt acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa
    12001 aaagcatttt ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg
    12061 tttgaaatta ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa
    12121 gaagaactgc actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt
    12181 ttagaagaaa tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga
    12241 gaagtgaaag atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg
    12301 gcatatgatc acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat
    12361 caactagact tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt
    12421 gctagcggat tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa
    12481 ggtgacttga tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca
    12541 cgtacattcg ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta
    12601 gatgctcaat tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat
    12661 gcagttgcta gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca
    12721 ggacatggga tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag
    12781 caatttgtac caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt
    12841 ggagtacgta ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt
    12901 tcaccaaaag aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca
    12961 gtgttcacag actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt
    13021 ttaagctgta agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg
    13081 atcggcggca gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta
    13141 tctcgtgctt aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa
    13201 acgcaaacca agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg
    13261 ggattgcagc ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg
    13321 tcactgaatt attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag
    13381 atggcttgaa agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag
    13441 caatggaaat gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat
    13501 ctgaagtcaa tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg
    13561 aagaactttt tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt
    13621 cgggaaatgg cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa
    13681 gatgcgattt tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca
    13741 gagtttgttc ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa
    13801 ttggataaag tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa
    13861 atggatttgg tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca
    13921 gcggctgttg ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca
    13981 cgtaaattcg tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa
    14041 gtcctagctt ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt
    14101 agaatgaaaa cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa
    14161 gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat
    14221 ggtatccatc ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat
    14281 gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat
    14341 cctgagacga tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct
    14401 agttttcatg gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc
    14461 ctgttagcta tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga
    14521 ctccttgcgg gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt
    14581 gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc
    14641 gtcggtaaac cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat
    14701 tctaaaaccg aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata
    14761 ggacgtggac attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta
    14821 gggatgaatc gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag
    14881 cctattgcag gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg
    14941 ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa
    15001 gaatatttaa ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct
    15061 tatttagaac gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc
    15121 catcaatatt tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga
    15181 gcattccaaa aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt
    15241 atttcattat atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat
    15301 ggaataggtg ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc
    15361 ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc
    15421 agtccaagcg gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt
    15481 gtccaattgg ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc
    15541 aggaatatcc agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg
    15601 ggtggttcga ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat
    15661 caagctaata cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat
    15721 ttagtagcag atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt
    15781 ttgtcagaag agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta
    15841 cgccagatcc agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg
    15901 cagcctgaaa gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta
    15961 tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac
    16021 caattggaac aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat
    16081 ctagaaaaac gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt
    16141 gctcaagctg ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac
    16201 agctatacga cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat
    16261 ttgctgacga tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa
    16321 ggagaataaa aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat
    16381 tgtcatgcag ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg
    16441 aggaatggaa ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac
    16501 gaaaatgatg acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga
    16561 tgagattaac tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt
    16621 ttttagatga gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag
    16681 caggcgggaa aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc
    16741 ctattgatgt tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct
    16801 tgatccacga tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca
    16861 atcataaagt attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag
    16921 cattccagct aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc
    16981 aattagctgt ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg
    17041 gcgaatcgaa aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc
    17101 atttgattcg ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct
    17161 tattgctact tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc
    17221 tactcgatgt gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta
    17281 tggaaaaaaa tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga
    17341 ttgagctgtt atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg
    17401 atggcgggct gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat
    17461 ggaaaaagaa cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca
    17521 agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa
    17581 accgggagaa aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta
    17641 tgtttctcga gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga
    17701 aggaaaaaca ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca
    17761 aaatggcgca aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat
    17821 aagacaagat cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga
    17881 agactttacc ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa
    17941 actgatgctg cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat
    18001 caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc
    18061 tcagacccat caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga
    18121 tgtgctggaa ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc
    18181 tcacttgagg aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt
    18241 ggtttcaaat gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg
    18301 agaagtatgc ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga
    18361 atatgctaag gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat
    18421 tgttgagtga gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag
    18481 gaatatctgt tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg
    18541 taccggcttc agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa
    18601 atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat
    18661 ttgttatttt gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac
    18721 gttacaagaa agaattattt tcgattatca atgatgacga taatgttttg atgattacac
    18781 gaactgaaga ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg
    18841 agcgaatgtg atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg
    18901 gctttctttt cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat
    18961 tattatcgat gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca
    19021 aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc
    19081 tgaactgatg gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga
    19141 tatgtcctta actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa
    19201 tctgagaaga atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact
    19261 gctccagcct gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca
    19321 gaagaaaaag agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg
    19381 aaaagtgcag caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca
    19441 agaagaaata gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag
    19501 agaaaaactt agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact
    19561 gacgaataat gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg
    19621 gatcgcccat ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta
    19681 tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga
    19741 gagtcgttta gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata
    19801 cggcgaatcc attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc
    19861 ttctgatttt tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct
    19921 tctacttcag caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt
    19981 ggaaaaatcc attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt
    20041 acgtttctta aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt
    20101 ttacattacg accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg
    20161 cgaactgtct cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac
    20221 aagtattatt tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc
    20281 agataaaatt tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca
    20341 agttgctgca gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac
    20401 gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct
    20461 ggctggaagt gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc
    20521 aaaaaggacc tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat
    20581 tttattttta catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca
    20641 gcattgtcca aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac
    20701 gcattgtttt caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt
    20761 ccacaagagg gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac
    20821 aaataaataa tgttaataga actagccata taaaggcatc atccctttca gcaataggta
    20881 acttaatgtt cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta
    20941 aagaagttaa aattttcata atattattca gattattcga tacgattgct gaaaaagtct
    21001 gattcatttg ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt
    21061 ccaccaatac atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt
    21121 gcgctagttc tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc
    21181 ttttttggat atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg
    21241 cttccaaagt atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga
    21301 cctgccaaat gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg
    21361 tctggctaaa gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct
    21421 ttttgggcgt caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac
    21481 ttgttttagc aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat
    21541 tgaattcttc tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg
    21601 gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat
    21661 accagatcgt ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa
    21721 aatatttgat catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat
    21781 tggaatacat ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat
    21841 gaaacagttg tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg
    21901 ttcatgggca cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg
    21961 tttggttgcc aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc
    22021 cctgatttgg cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg
    22081 acaatttcta tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct
    22141 gttttccaag catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg
    22201 cgtttggttt ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt
    22261 tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct
    22321 tgatttttaa aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt
    22381 ggtagaaagt gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc
    22441 aatgttgatg ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc
    22501 aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct
    22561 tttcggctat ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt
    22621 ggctaaaaaa gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga
    22681 acttgataaa caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac
    22741 aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg
    22801 gcaagatttt tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga
    22861 tttcggacta taagaatagg aagaggctga ctaatgactg aaacatttca acactataca
    22921 gtcatgttga aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac
    22981 tgtacactcg gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg
    23041 catctgtatg cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct
    23101 tcttatgtcg aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa
    23161 cgcttgagtg agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct
    23221 cctcagctag atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg
    23281 cgcatggatc agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat
    23341 gaattagtaa agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt
    23401 gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc
    23461 attaaacaag tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt
    23521 tttcaagctg tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa
    23581 caggcaatcc ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg
    23641 gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg
    23701 ggacttcctg tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca
    23761 atccttcctt cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt
    23821 attgcagaaa aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca
    23881 agattacgaa tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc
    23941 ttcttcgaat caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc
    24001 tgctttggaa aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt
    24061 aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac
    24121 agaaatcaat gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc
    24181 tcgtactgaa cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa
    24241 tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa
    24301 aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat
    24361 tggattgttc tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc
    24421 tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc
    24481 aaaaagagga acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta
    24541 ttcaattaaa gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga
    24601 agaaaagaat tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa
    24661 agatgcactg aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg
    24721 tagctatggg aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa
    24781 agaaggtgtc gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt
    24841 cttttcttct cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg
    24901 taaactaggt ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat
    24961 ttatcaaaaa gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc
    25021 tgtcgacgga caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac
    25081 actgatggat caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa
    25141 agcaaaaacc ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat
    25201 ggaaggattg actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga
    25261 accaggatca accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa
    25321 cgagaatgaa accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca
    25381 tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt
    25441 cgggatggtc atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact
    25501 gggatttgga caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac
    25561 attaaatatt gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt
    25621 ccaaatgatg aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata
    25681 tatcagcaaa gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct
    25741 agggcaacct ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt
    25801 cgaaagcgaa aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc
    25861 ggcaaaaaac ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac
    25921 agcctacctg tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta
    25981 tctaacaatg aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc
    26041 attgatgaaa cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa
    26101 aacagaaaaa gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga
    26161 tgctcaaaaa agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca
    26221 atctactgcc aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg
    26281 tgataagctg atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa
    26341 aatcctcggt attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc
    26401 tccatatgaa aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa
    26461 ataggagaga aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg
    26521 caatgacaat tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc
    26581 aggcgattcg tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg
    26641 gcggcttagt gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa
    26701 aacagctaac tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg
    26761 gctttcttga tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc
    26821 agaaattgat tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag
    26881 gttatccagg tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta
    26941 ttttcgctct cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg
    27001 atggattagt tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc
    27061 atcagcatca gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt
    27121 tcttcgtcta caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt
    27181 tgggcggact tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga
    27241 ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc
    27301 ttacaggaaa acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat
    27361 ggtcggagtg gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca
    27421 cattatggat cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat
    27481 aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat
    27541 gaattgggcg cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca
    27601 caagagctgc tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg
    27661 gatgaagaat tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta
    27721 gcaaaagccc aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta
    27781 gcggaatgcc caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg
    27841 acaggactac tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt
    27901 gggtatccag caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa
    27961 ttgtctagct tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc
    28021 aatatctatg aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa
    28081 tggaaccttc aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca
    28141 gaactacaag aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa
    28201 gtgttggaag acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata
    28261 atggaaatat ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct
    28321 atttctgtag caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt
    28381 ttccatggcg taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat
    28441 aatgattcaa aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg
    28501 tctaaggtcg tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt
    28561 ccttcattaa aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg
    28621 gatgctggaa aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca
    28681 gttcctttag cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat
    28741 gccagctggg atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg
    28801 aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg
    28861 cggaggaaca gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca
    28921 agaaccgaat gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt
    28981 ccctgataca ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc
    29041 catgcataac atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat
    29101 cctcagtgat tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt
    29161 agtgtacgct gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc
    29221 aggtgttacc aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga
    29281 tgcggcttcc ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga
    29341 agtagcagat actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa
    29401 aacagtcttg atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt
    29461 ttttttaagt gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata
    29521 ttatgaagag atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta
    29581 tatcaaaaat atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc
    29641 aacttcgatc gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata
    29701 tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa
    29761 aatgattgca gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat
    29821 gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga
    29881 tgcttcaaaa cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc
    29941 ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat
    30001 ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa
    30061 gagccaaaaa aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa
    30121 acggttcaag aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa
    30181 gagatcaagc aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc
    30241 aatttaaaaa gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta
    30301 ttgggtatac cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca
    30361 gttgcagtat caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg
    30421 tcattaggag aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt
    30481 aaaaaagcac agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc
    30541 gatttggatg taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac
    30601 ccagtgattg aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc
    30661 cctatcttgg aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat
    30721 cagttatcat cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc
    30781 aataaaaatc tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat
    30841 aatcttgcga accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt
    30901 gtgatcgaca tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa
    30961 gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa
    31021 tcaagtgaag aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga
    31081 tcatctgatt caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact
    31141 ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa
    31201 aaaccagtaa tttatggtag aattggaagc agtgaatata tctattaata agtatatttt
    31261 aaaacggacg ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca
    31321 tggcaaaaac aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg
    31381 tggctgaata tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag
    31441 ggattaaccg aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag
    31501 tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag
    31561 caaacatgct tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg
    31621 aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc
    31681 ctgaacgtca aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa
    31741 tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag
    31801 gacctaaaac gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca
    31861 acgaaatggt gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag
    31921 attttggtac gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg
    31981 ataagcaatt gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata
    32041 tctctattgt attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg
    32101 atgcataccc agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt
    32161 cagaaccagt gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac
    32221 agattttcaa caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg
    32281 gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa
    32341 tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat
    32401 ttacaaatgt aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg
    32461 caaaaattgc tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa
    32521 caacaaacac atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac
    32581 caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg
    32641 attaattcat cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac
    32701 ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg
    32761 attgaagaaa acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta
    32821 aaaaattcaa aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt
    32881 gctggttctc agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc
    32941 gaagctttag acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt
    33001 acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt
    33061 gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt
    33121 atcgctcgtt tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta
    33181 gaagttgtag ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta
    33241 cgacaaggtg tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac
    33301 tttgctgacg tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg
    33361 gctagtggag aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt
    33421 gaaacatcta ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg
    33481 acattgttcg aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta
    33541 aatatcattc taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc
    33601 atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa
    33661 gcacaaatgc aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact
    33721 tcttcagaag aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt
    33781 cctagagttg atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac
    33841 cgcgaagaaa caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt
    33901 aaaaaataaa ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga
    33961 aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt
    34021 aacaaaatct gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg
    34081 tgcagaaaac cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga
    34141 aataaagtgg catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga
    34201 aattgattac tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt
    34261 agagaaagaa ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg
    34321 aatttcacca gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa
    34381 ggttgttggg ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt
    34441 gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc
    34501 atgtagtgaa ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc
    34561 aacatttatc cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc
    34621 cgcttttaaa cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg
    34681 aataccaaga ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa
    34741 cacagaatca atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg
    34801 cgacttcacg tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg
    34861 aactacatca atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta
    34921 cacaacctaa agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg
    34981 aagctatggc gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt
    35041 tagtagaaga aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac
    35101 tagatggaga tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg
    35161 agattgacag ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg
    35221 aggaactacg atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt
    35281 ctatagcatt atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc
    35341 agggctagga cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct
    35401 gcctttgagg attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt
    35461 agccgtacaa ggtttagcac agatcgttat atggataact gtccatttag tttattgatt
    35521 gaagcaacaa aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt
    35581 ataccagcac tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga
    35641 acaggttcaa ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta
    35701 tattttagaa acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata
    35761 tgaacaggca gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga
    35821 agattttgaa acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca
    35881 tgggaaaatc cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat
    35941 catctcggac ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc
    36001 tgcacaagta acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga
    36061 aatgattatg ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact
    36121 agacagtgtg atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga
    36181 agctggtaaa gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact
    36241 attagatatt atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa
    36301 gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt
    36361 ggtttactca tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg
    36421 cggggatata atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag
    36481 gaattgactc aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt
    36541 gagtacttca atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg
    36601 gctgataaag taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa
    36661 aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat
    36721 gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg
    36781 acagaaactg aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg
    36841 cttgagtctc aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc
    36901 tccagctacg tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac
    36961 aatgaaaatg atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca
    37021 tttgatacac aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac
    37081 agaaaagcac tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact
    37141 gcatggtgaa agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg
    37201 gctgacctcg ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg
    37261 gtaccacggc atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat
    37321 aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc
    37381 cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat
    37441 cgaatctacc aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat
    37501 ggacctccat atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa
    37561 gacattatca ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg
    37621 gatacacatg gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa
    37681 atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag
    37741 caacgagcag atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca
    37801 ttagatcctt cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc
    37861 tatatttaca aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg
    37921 gaagctgaaa ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc
    37981 aaagacggca aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca
    38041 tggacattgc cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg
    38101 gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa
    38161 atcggttggg aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg
    38221 actgcaagtc atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact
    38281 ttagacgctg gtactggatt agtccatact gctccaggac atggggaaga tgactatatc
    38341 gctggtagca aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca
    38401 gaagaagcac ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat
    38461 ctattaaaag aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac
    38521 gattggcgga cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc
    38581 gataaattcc gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg
    38641 ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag
    38701 cgtgcatggg gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca
    38761 ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt
    38821 gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc
    38881 gaatttacaa aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa
    38941 gctgttttac gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca
    39001 gaccaatatc gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta
    39061 gcaccataca aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg
    39121 agtaaatctt taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat
    39181 atccttcgct tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat
    39241 atcttaagtc aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta
    39301 gcgaatacag aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct
    39361 gtagacaaat atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg
    39421 tacgacaaat ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat
    39481 ctatcgtcat tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat
    39541 tatcaacgtc gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta
    39601 ttgactccta tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag
    39661 gagtacgttc agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg
    39721 atggatacct ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa
    39781 gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa
    39841 caacaagttc gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt
    39901 tcaccagact actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta
    39961 tttgatgatg ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc
    40021 caagttcgta aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca
    40081 aaaatcgttg aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag
    40141 ggaaaagggg gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt
    40201 tccacgttca cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag
    40261 tagttcgaaa gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg
    40321 tgtttggtcc caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc
    40381 ccaatgagag aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc
    40441 ttctgggcta ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt
    40501 ttcaaatcct tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg
    40561 aatatagatc gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga
    40621 tgttttgaat acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc
    40681 agtcaaacgt ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat
    40741 agtgaatttg cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc
    40801 gcgatagccc ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa
    40861 gttttctaac acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc
    40921 tgtacggatt tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac
    40981 ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc
    41041 gcgatcttct acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt
    41101 gttccattga gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat
    41161 ttcttttcct aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat
    41221 ttgctggttc aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa
    41281 acgattgaag ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc
    41341 aaaaaattga ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc
    41401 atctaatcgg tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg
    41461 ggattgatag taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc
    41521 acggattgtt tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt
    41581 tccgattaca gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg
    41641 gtacaattta cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt
    41701 ttcattagac atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc
    41761 ttaaagctga cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct
    41821 gcgatgtcaa cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc
    41881 aacaattctt tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac
    41941 tccaatgttt gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca
    42001 tgaggacaat gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa
    42061 gtcacatgtt ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt
    42121 tcaactaaaa aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt
    42181 ccgcttgcag tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca
    42241 agtttagaaa tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt
    42301 ttattgttga ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct
    42361 cgatttggtg tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa
    42421 caaaaaaaac gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt
    42481 tacaacaaaa aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata
    42541 tatacattgg gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg
    42601 tcataaagag tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg
    42661 actaaacgat attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa
    42721 aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat
    42781 gtaccttgat actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa
    42841 ataaaacttg agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc
    42901 aaatcactcc tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg
    42961 taccgaataa aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta
    43021 caaattatga taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt
    43081 ggaaatagct ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga
    43141 cggagaaata attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc
    43201 aggcgctgaa gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct
    43261 gattgctgta aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct
    43321 ggcaattgat ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac
    43381 ggtatacaag cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt
    43441 ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg
    43501 tgcttttcct gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc
    43561 cgcacaagat ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct
    43621 ggcaacgatc catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca
    43681 aagcggagtt caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa
    43741 gcaggccatt ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg
    43801 taaaataatg atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca
    43861 agcagcttta agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa
    43921 agcacaggaa tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac
    43981 ttttttcaat attactcata tggacaccat ttatattgct tctccgaata gtcttcactt
    44041 tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt
    44101 ttcgacgcca aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt
    44161 ttttgaagca gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc
    44221 attaaaagac gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga
    44281 ccaagtatta gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt
    44341 gatagattta ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga
    44401 agtccattat ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt
    44461 acgttatgag aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc
    44521 gtcagagatt tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca
    44581 agcacaattt catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga
    44641 aaatccaatg gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc
    44701 agcttgggga ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat
    44761 ttatgattta agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta
    44821 atgaacgaat ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg
    44881 tttatctcac cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt
    44941 atagtagcgg tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta
    45001 ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta
    45061 gctattcaag tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa
    45121 tctttggtgg gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa
    45181 atcttgattg gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca
    45241 catcaagtga aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc
    45301 caaatcattg atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca
    45361 gcgacagcag atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca
    45421 attgacgtga cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta
    45481 ccgatacgaa agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt
    45541 ctgattttct ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga
    45601 ggtgtaccgg taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta
    45661 gaacaattca aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt
    45721 ttagatatta caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct
    45781 tatttgcatc gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc
    45841 gtacaagata ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt
    45901 gaagagattt ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca
    45961 gctcctgcag taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa
    46021 tataacaaag aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa
    46081 ggagcaaggc gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg
    46141 gttgccagag gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag
    46201 aaaaccaaca aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca
    46261 atgcaagccg atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc
    46321 gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact
    46381 cttctacgca agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca
    46441 tacagcaggc aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg
    46501 gagaaactgg tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt
    46561 cagagatgct ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa
    46621 atccgcaact gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag
    46681 ctcaagatag tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga
    46741 tccaccgttt accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt
    46801 accgtccgct tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga
    46861 cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca
    46921 ccttgccgaa tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta
    46981 tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg
    47041 ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag
    47101 tctgtgcaaa atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa
    47161 aagaagaact ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac
    47221 gtctcgaccg attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg
    47281 atgttgtctg gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa
    47341 aagtcacttc tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa
    47401 aagatgtgga tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca
    47461 aagatctttt gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa
    47521 aacggttaga ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa
    47581 gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac
    47641 ggaaaaattt taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag
    47701 aagaacaagt tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca
    47761 atctaggaga aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg
    47821 gcagacagac tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag
    47881 ggaaagcttt taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac
    47941 aatatgtcaa aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg
    48001 gatcattaaa caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta
    48061 tcacgatgac aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa
    48121 gcttagaaga tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt
    48181 tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta
    48241 ctggtgaagg agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata
    48301 aggtagaact gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg
    48361 ccttattgga agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa
    48421 atatcccatt tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc
    48481 gtatagctga tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa
    48541 gcgatgtctt ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc
    48601 agaccgattt gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag
    48661 ccttcatgtt agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta
    48721 ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag
    48781 aacgaaaata tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc
    48841 ttgaaaataa aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt
    48901 gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat
    48961 tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca
    49021 aaaaaaacaa tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa
    49081 atgcaaaaag cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca
    49141 gatgcagata ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta
    49201 cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc
    49261 atcaatatgc aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat
    49321 ctaatgaaat aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc
    49381 tagaagccat catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag
    49441 cgattgcggc tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag
    49501 aagaatcact tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag
    49561 gaaatcatat gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta
    49621 ttgcgtttac tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat
    49681 aaatacaact tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt
    49741 tataaggaga actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact
    49801 atcaagttgg atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca
    49861 tttagttgct ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg cgggatcagt
    49921 cgctgtaatg atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc
    49981 tgacagttta gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa
    50041 tggttttgtc tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg
    50101 gtataatctt gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc
    50161 taccttgatt caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt
    50221 tccttcaaat gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg
    50281 cacactgtca gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta
    50341 ttttacagtt atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc
    50401 caacattacg atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc
    50461 cattttacca gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg
    50521 tgtagctgat gggcaactga tcattgattt ggtgcaaaaa tatattgcag aaccattcct
    50581 aggattatct caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg
    50641 gttctttggt attcatggac caaatgtatt ggcaccagtg ctagagggaa tctggggaca
    50701 agcacaacta atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc
    50761 agccgtgtta gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga
    50821 tgcttttgca tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat
    50881 tttctctaaa cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt
    50941 caatatcaac gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat
    51001 cccattcttg attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg
    51061 cctagtcgct ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt
    51121 cttagcaaca ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac
    51181 tttcttgatt tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga
    51241 acaacaataa gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt
    51301 ttcgaggagg aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag
    51361 aattgttaga aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa
    51421 tcaagctgtt ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg
    51481 aactaatcaa agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga
    51541 aaatggttta aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta
    51601 ttggttcttt gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata
    51661 gtatggtcac aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa
    51721 aaagagaaga agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc
    51781 agcatcgttt ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac
    51841 cggcaaatca gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca
    51901 aaataaccgg gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg
    51961 tacatcgaca acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc
    52021 gacctgtgag gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa
    52081 tgatatgctt ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct
    52141 agatatgatc gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa
    52201 atgaagtaag ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact
    52261 agcttttttg taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct
    52321 tcgattgagg aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa
    52381 aggcgagaac gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag
    52441 gaggatcggc taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa
    52501 gagtacctaa tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc
    52561 gggatcaatc agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta
    52621 tcttatctgg cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt
    52681 gataaaatca gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga
    52741 aaaataaagc gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag
    52801 atgccggaaa accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc
    52861 aagcaaaaag gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga
    52921 tatatcgttt atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat
    52981 catgatgctt tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca
    53041 tttgaatatt catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt
    53101 gcttctgtaa tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg
    53161 gaaaattatc catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat
    53221 tatgcgaaat ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc
    53281 tcaaaagtag aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag
    53341 ctattattag ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca
    53401 aaagaatatg aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac
    53461 tatttttgat aaaaaacggg tgatgacaat
//
LOCUS       DACEKH010000007        52801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEKH010000007 DACEKH010000000
VERSION     DACEKH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.007"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(128..976)
                     /locus_tag="IW662_000357"
     CDS             complement(128..976)
                     /locus_tag="IW662_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0865208.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1200..3140)
                     /gene="thrS"
                     /locus_tag="IW662_000358"
     CDS             complement(1200..3140)
                     /gene="thrS"
                     /locus_tag="IW662_000358"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ0865209.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3477..3656)
                     /locus_tag="IW662_000359"
     CDS             complement(3477..3656)
                     /locus_tag="IW662_000359"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ0865210.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3703..5526)
                     /gene="cadA"
                     /locus_tag="IW662_000360"
     CDS             complement(3703..5526)
                     /gene="cadA"
                     /locus_tag="IW662_000360"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0865211.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5804..8083
                     /locus_tag="IW662_000361"
     CDS             5804..8083
                     /locus_tag="IW662_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0865212.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8176..8823)
                     /locus_tag="IW662_000362"
     CDS             complement(8176..8823)
                     /locus_tag="IW662_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865213.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9002..9721)
                     /locus_tag="IW662_000363"
     CDS             complement(9002..9721)
                     /locus_tag="IW662_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0865214.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9852..11003)
                     /locus_tag="IW662_000364"
     CDS             complement(9852..11003)
                     /locus_tag="IW662_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0865215.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11220..11960)
                     /locus_tag="IW662_000365"
     CDS             complement(11220..11960)
                     /locus_tag="IW662_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0865216.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11964..12287)
                     /gene="rsfS"
                     /locus_tag="IW662_000366"
     CDS             complement(11964..12287)
                     /gene="rsfS"
                     /locus_tag="IW662_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ0865217.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12344..12937)
                     /locus_tag="IW662_000367"
     CDS             complement(12344..12937)
                     /locus_tag="IW662_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0865218.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12921..13568)
                     /locus_tag="IW662_000368"
     CDS             complement(12921..13568)
                     /locus_tag="IW662_000368"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ0865219.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13581..13892)
                     /gene="yhbY"
                     /locus_tag="IW662_000369"
     CDS             complement(13581..13892)
                     /gene="yhbY"
                     /locus_tag="IW662_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ0865220.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13908..15017)
                     /gene="yqeH"
                     /locus_tag="IW662_000370"
     CDS             complement(13908..15017)
                     /gene="yqeH"
                     /locus_tag="IW662_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ0865221.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15014..15541)
                     /locus_tag="IW662_000371"
     CDS             complement(15014..15541)
                     /locus_tag="IW662_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ0865222.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15721..16506)
                     /locus_tag="IW662_000372"
     CDS             complement(15721..16506)
                     /locus_tag="IW662_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ0865223.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16499..17368)
                     /locus_tag="IW662_000373"
     CDS             complement(16499..17368)
                     /locus_tag="IW662_000373"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ0865224.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17369..18748)
                     /locus_tag="IW662_000374"
     CDS             complement(17369..18748)
                     /locus_tag="IW662_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ0865225.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18756..19175)
                     /gene="fabZ"
                     /locus_tag="IW662_000375"
     CDS             complement(18756..19175)
                     /gene="fabZ"
                     /locus_tag="IW662_000375"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0865226.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKTSAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19200..19676)
                     /gene="accB"
                     /locus_tag="IW662_000376"
     CDS             complement(19200..19676)
                     /gene="accB"
                     /locus_tag="IW662_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ0865227.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19679..20914)
                     /gene="fabF"
                     /locus_tag="IW662_000377"
     CDS             complement(19679..20914)
                     /gene="fabF"
                     /locus_tag="IW662_000377"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ0865228.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20933..21670)
                     /gene="fabG"
                     /locus_tag="IW662_000378"
     CDS             complement(20933..21670)
                     /gene="fabG"
                     /locus_tag="IW662_000378"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ0865229.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21682..22599)
                     /gene="fabD"
                     /locus_tag="IW662_000379"
     CDS             complement(21682..22599)
                     /gene="fabD"
                     /locus_tag="IW662_000379"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0865230.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22706..22930)
                     /locus_tag="IW662_000380"
     CDS             complement(22706..22930)
                     /locus_tag="IW662_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0865231.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22983..23945)
                     /locus_tag="IW662_000381"
     CDS             complement(22983..23945)
                     /locus_tag="IW662_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ0865232.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23955..24395)
                     /locus_tag="IW662_000382"
     CDS             complement(23955..24395)
                     /locus_tag="IW662_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0865233.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24701..25666
                     /locus_tag="IW662_000383"
     CDS             24701..25666
                     /locus_tag="IW662_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0865234.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25723..25953)
                     /locus_tag="IW662_000384"
     CDS             complement(25723..25953)
                     /locus_tag="IW662_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ0865235.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26039..26926)
                     /locus_tag="IW662_000385"
     CDS             complement(26039..26926)
                     /locus_tag="IW662_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0865236.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27015..28238)
                     /locus_tag="IW662_000386"
     CDS             complement(27015..28238)
                     /locus_tag="IW662_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0865237.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28255..29334)
                     /locus_tag="IW662_000387"
     CDS             complement(28255..29334)
                     /locus_tag="IW662_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0865238.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29465..31192)
                     /gene="ptsP"
                     /locus_tag="IW662_000388"
     CDS             complement(29465..31192)
                     /gene="ptsP"
                     /locus_tag="IW662_000388"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ0865239.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31192..31458)
                     /locus_tag="IW662_000389"
     CDS             complement(31192..31458)
                     /locus_tag="IW662_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ0865240.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31937..34171
                     /locus_tag="IW662_000390"
     CDS             31937..34171
                     /locus_tag="IW662_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0865241.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34319..34639
                     /locus_tag="IW662_000391"
     CDS             34319..34639
                     /locus_tag="IW662_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0865242.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34844..35068)
                     /locus_tag="IW662_000392"
     CDS             complement(34844..35068)
                     /locus_tag="IW662_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ0865243.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35266..36846)
                     /locus_tag="IW662_000393"
     CDS             complement(35266..36846)
                     /locus_tag="IW662_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ0865244.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37247..38623)
                     /locus_tag="IW662_000394"
     CDS             complement(37247..38623)
                     /locus_tag="IW662_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0865245.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38751..39869)
                     /locus_tag="IW662_000395"
     CDS             complement(38751..39869)
                     /locus_tag="IW662_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0865246.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            40011..40445
                     /locus_tag="IW662_000396"
     CDS             40011..40445
                     /locus_tag="IW662_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0865247.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40523..41056)
                     /locus_tag="IW662_000397"
     CDS             complement(40523..41056)
                     /locus_tag="IW662_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ0865248.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(41150..42328)
                     /gene="mutY"
                     /locus_tag="IW662_000398"
     CDS             complement(41150..42328)
                     /gene="mutY"
                     /locus_tag="IW662_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ0865249.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42321..43118)
                     /gene="recX"
                     /locus_tag="IW662_000399"
     CDS             complement(42321..43118)
                     /gene="recX"
                     /locus_tag="IW662_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ0865250.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43225..44607
                     /gene="rlmD"
                     /locus_tag="IW662_000400"
     CDS             43225..44607
                     /gene="rlmD"
                     /locus_tag="IW662_000400"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0865251.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44781..45359)
                     /locus_tag="IW662_000401"
     CDS             complement(44781..45359)
                     /locus_tag="IW662_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ0865252.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45681..46151
                     /locus_tag="IW662_000402"
     CDS             45681..46151
                     /locus_tag="IW662_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ0865253.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46259..47485)
                     /locus_tag="IW662_000403"
     CDS             complement(46259..47485)
                     /locus_tag="IW662_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ0865254.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48239..48439)
                     /locus_tag="IW662_000404"
     CDS             complement(48239..48439)
                     /locus_tag="IW662_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0865255.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48573..49181)
                     /locus_tag="IW662_000405"
     CDS             complement(48573..49181)
                     /locus_tag="IW662_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ0865256.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49266..49736)
                     /locus_tag="IW662_000406"
     CDS             complement(49266..49736)
                     /locus_tag="IW662_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ0865257.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49784..50698)
                     /locus_tag="IW662_000407"
     CDS             complement(49784..50698)
                     /locus_tag="IW662_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0865258.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50844..51647
                     /locus_tag="IW662_000408"
     CDS             50844..51647
                     /locus_tag="IW662_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ0865259.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51825..52772
                     /locus_tag="IW662_000409"
     CDS             51825..52772
                     /locus_tag="IW662_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0865260.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT        15246 a        11125 c         9016 g        17414 t
ORIGIN      
        1 attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa cactttgatg
       61 gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat aaaaaatagt
      121 aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt tttttcactt
      181 tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg atcgtagcaa
      241 attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt ggtctagatg
      301 tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc gtcaacgcaa
      361 tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct gcacttctac
      421 ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt tgcgggagca
      481 agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat ttttgcagaa
      541 tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc tctgccgatt
      601 ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact gtttctttta
      661 tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac tcatttgctt
      721 ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc gtgattttga
      781 agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc ggcgcactca
      841 acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca atattaccga
      901 taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata ctttcaatac
      961 gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta agtataccat
     1021 atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat ccgcccaaca
     1081 gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt tgaggaatac
     1141 ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa atttacctat
     1201 tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa gatattcaaa
     1261 tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc atcttccaat
     1321 tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc acgtatctta
     1381 tagcccattt tttcattacg atcatcaact tctacacgca accccttcat ttgcaaacgt
     1441 tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat gattgttgct
     1501 tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa ataagcgaca
     1561 aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg cgtattttcc
     1621 ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc taattgtata
     1681 gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag ttttggtccg
     1741 taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc catagcggct
     1801 ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt atctgtattg
     1861 tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc agcatatact
     1921 gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg acgaacaaat
     1981 gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa agcacctgat
     2041 ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc acgataagaa
     2101 tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg aagaacaagc
     2161 atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg atcccagtgt
     2221 ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg ttggtaacct
     2281 agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt tgcccctttt
     2341 ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa gtcaagttct
     2401 tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat atattctttc
     2461 aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg attgtttgag
     2521 tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat acggccagtg
     2581 gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata agcagtaatc
     2641 acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact tgcaaaaatt
     2701 tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc tttgacgatt
     2761 ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt ttgtccatta
     2821 tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg gatatttgga
     2881 tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac taagccaagt
     2941 gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat tggacgagtc
     3001 aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa gctttttgaa
     3061 atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac tgcaccatct
     3121 ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg atcagatcat
     3181 caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg acgaaaagtt
     3241 cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa cggtccgacc
     3301 gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc tggaaaagtt
     3361 tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt tctcatcaat
     3421 caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa ctaaacttat
     3481 tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat gatgacatgg
     3541 atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc tttcatcata
     3601 ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa tggcataatg
     3661 ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta tgtttgtctt
     3721 gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac tcaaaataga
     3781 gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta gtcccgtact
     3841 tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact gtttcgctat
     3901 tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat catctttcaa
     3961 gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca ttgcaatacc
     4021 gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca tcgcaacagg
     4081 tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca agctacgggc
     4141 atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag atccatcccc
     4201 ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg tctttgctgc
     4261 ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat caattgttac
     4321 atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg cacgtccaat
     4381 ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt ctttcagttg
     4441 actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag ctaagatatg
     4501 gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt ttattccatt
     4561 gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg ttttatcaaa
     4621 agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta caacgatccc
     4681 gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta gaatcagtgg
     4741 acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg agaaccacca
     4801 agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac gatcagctaa
     4861 acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa tcatttgata
     4921 ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc catttaaaga
     4981 ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac ctgtcaaaga
     5041 agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta caagttcgcc
     5101 aggcttgact agtaactggt catcaatatt tatttcatcg attactatat cctgcaattt
     5161 tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact gcagttcttt
     5221 acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca tgatcaatat
     5281 catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg taatcgctaa
     5341 tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa acatcaagaa
     5401 ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct ggtgaaaaac
     5461 aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt ttttgaaatg
     5521 tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa atttccagct
     5581 gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata ggccttaatt
     5641 gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac aaaaatattc
     5701 agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa aaactatgct
     5761 atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg agaaacaact
     5821 agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa atatgctgga
     5881 tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca cagctaatca
     5941 caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat atcgccaaca
     6001 tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac gcctgcaaac
     6061 gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag aagaccgtga
     6121 aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag aaacaatcgt
     6181 tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg gtcccgcatt
     6241 ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac aatttaaaat
     6301 ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg atgacttttt
     6361 gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct ctacgataca
     6421 aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga ttgaaggaat
     6481 cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt tgtatcataa
     6541 tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt tgttttctga
     6601 ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac agacattcaa
     6661 aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc aacaagaaag
     6721 tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat tgaccttagt
     6781 cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt tccgtgatat
     6841 gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt ataaagaaac
     6901 aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc tcctgaaaaa
     6961 attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc tggcggaaga
     7021 acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac aaaaggagcg
     7081 ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc gaatccatcg
     7141 agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact ttctacaaac
     7201 tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc aggaacattt
     7261 gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg ctgtactatt
     7321 ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc gctatatctt
     7381 tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag ccttgttccc
     7441 aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa atgaaacgat
     7501 cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc aattaacgac
     7561 aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg tgaaagaaca
     7621 tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag gaacaacaga
     7681 tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa aagcccggta
     7741 ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt atgctcaact
     7801 gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact ttatgaagcg
     7861 ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc gagttttctt
     7921 gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc tttacaccat
     7981 gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa gtcctttgtt
     8041 aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa gacaactgct
     8101 atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa aagtaattca
     8161 atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat attttaattt
     8221 gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg ggtcaaatac
     8281 cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat tccattcaat
     8341 gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca aaatctctga
     8401 tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt ctatcttctc
     8461 atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta atagtagaaa
     8521 gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag aacgacgaaa
     8581 aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt ccaccataca
     8641 ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat agccttcaac
     8701 gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca cagctaactc
     8761 ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga tttttgcttc
     8821 catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat acttatgctc
     8881 tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag caagacaatc
     8941 tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta aaactggttg
     9001 cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga tcttccagtg
     9061 catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc tcgttttgag
     9121 ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt aatgcttctt
     9181 gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct tcttccatta
     9241 catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca gcgtcttctc
     9301 ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg gaaacaccca
     9361 tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga ttcacattgt
     9421 tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca tagcgcaatt
     9481 cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca ataatatgtt
     9541 ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg tttgcatgcg
     9601 gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt gcataaatcc
     9661 ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat ttacgtccca
     9721 tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc tgttccatta
     9781 tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc ttctcatttt
     9841 tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg aatattgcct
     9901 cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc ctcttttttt
     9961 ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct tccagcatcg
    10021 gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc ttgctttttg
    10081 atattcagca agacataaca agataatcgt tggatccgtg tccatgtata tctttttgtt
    10141 tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt tttcatgcgc
    10201 gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt agaagataac
    10261 aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca agttgaaaga
    10321 tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc taacgcatgg
    10381 cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg atagcctgca
    10441 cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt ttcctttgca
    10501 taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc gggatacaga
    10561 ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga cagttcttga
    10621 aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact gctgtaatca
    10681 aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt ttgcaagatt
    10741 tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac gggaagctcc
    10801 actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt atcgatgata
    10861 gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac ctctgctcct
    10921 gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg attgtactct
    10981 acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata aaaaagaaat
    11041 agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata aactattctc
    11101 gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct atatcgttcg
    11161 gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag gcaaaaccgc
    11221 tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc ttcgtctgta
    11281 aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct taaataactt
    11341 tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg accagaccct
    11401 tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc gcttcctgga
    11461 taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata ttctggaaaa
    11521 acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt ttcttctaac
    11581 gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat atagcacagc
    11641 gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc gagcatattg
    11701 ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc aatcatcaac
    11761 atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa ggcaaccgct
    11821 aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg taaatgacgt
    11881 ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc gtaaatgaaa
    11941 gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac tagcggagca
    12001 tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa aacatgtacg
    12061 atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc aatacgtttt
    12121 acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc gttgatttga
    12181 cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac atttcgaacg
    12241 tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc aatttttaat
    12301 tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt tttgctaccc
    12361 agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca agcaaatggg
    12421 ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat gcaatggctc
    12481 gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct acataaatga
    12541 ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt gcatgaagaa
    12601 tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca agaccatgcc
    12661 agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg attaccattt
    12721 tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca atactagctt
    12781 tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct actccaagca
    12841 catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg ttttctctag
    12901 aataagcagt atattcatta ctatagtcca tattcataca acccttcatt ttgaatataa
    12961 tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat cttttgacgt
    13021 attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg atatggagta
    13081 tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt cagctcatcg
    13141 attttatacc attttggcaa atattctacc atatccccac cgataataaa gtaataatct
    13201 gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact tttcccacca
    13261 cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc taattccaac
    13321 atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg tggctgataa
    13381 gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc agccatcact
    13441 aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg ctttttcttc
    13501 tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt cttagcttgc
    13561 gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct ttgatacttt
    13621 tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac gatttcgcaa
    13681 tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt attttgaagt
    13741 aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac gatcattgct
    13801 gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc tttgctacgt
    13861 aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta aatcaatgct
    13921 ttgcgtttca atacatctac accctttggc gcccagcccg caattactac tggcgctgtt
    13981 acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac agaaaactca
    14041 aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg taataatcca
    14101 cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg aatatttaca
    14161 tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc aaatcttgca
    14221 agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg tttgatttct
    14281 tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat ttgatggcga
    14341 tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat ctcgatttta
    14401 tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc cgctgtattt
    14461 ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac gatatagaca
    14521 tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc atgagatttc
    14581 ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc acgttccttc
    14641 atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac tttgttccca
    14701 actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag tgaaccattg
    14761 aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa ttcattcagt
    14821 aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata atggcgtaat
    14881 ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa agccgtttgc
    14941 ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca gccgatacat
    15001 tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt tttgctaata
    15061 gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa ctatctgttg
    15121 cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca cgtatatcag
    15181 tcataatctg atcgcctacc attactatct catcatctgc taaatccaat tgtttctttg
    15241 ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa agatcaaatt
    15301 tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca acagggattc
    15361 ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct ggattattcc
    15421 atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga ttctttaatt
    15481 gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa tttgaaaaca
    15541 tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca accccgtatt
    15601 atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag aaaaagcatt
    15661 tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa caaaaaacaa
    15721 ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat ctaatcgaac
    15781 aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt tatatcgtgg
    15841 caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta actcgcgtag
    15901 ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac cgtctttcca
    15961 caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt ccagcatcca
    16021 gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac caatgatgat
    16081 ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa tagcctctcc
    16141 ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga tcaaggtaat
    16201 caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc gatagccttc
    16261 aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc ctttttgaat
    16321 accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa tcgcttcgtc
    16381 atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct cttcaaaaaa
    16441 atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat gtgctgtctt
    16501 atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg attttctgtt
    16561 tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag aattcagcct
    16621 tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt cttccagcaa
    16681 aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca aaactggcag
    16741 ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct tcattgctat
    16801 gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt ccttcttgca
    16861 tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt gttgctcgtt
    16921 cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg ataaaattag
    16981 gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca gtgaggactg
    17041 cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat tcgggaaatg
    17101 ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt tcatcaataa
    17161 tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga gggcatactt
    17221 tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac tttgcccaca
    17281 tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga ttgatccgaa
    17341 tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt tctggtgtcc
    17401 aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct atgactgcag
    17461 gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct gtgataaatt
    17521 cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca tggacgatta
    17581 tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa actgcactgt
    17641 ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc cctggagaag
    17701 gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg tgtccttgga
    17761 atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc acctgttttt
    17821 ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg attcgagtat
    17881 tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc gttccggcat
    17941 tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct tctctttttg
    18001 tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg cgttgtaaag
    18061 aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag atctgaactt
    18121 ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt ccaaatgccg
    18181 ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt gattgaactt
    18241 tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga taaccaattc
    18301 tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt cctggaatga
    18361 ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc attgcatcga
    18421 tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg gaggcaaatc
    18481 ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt gtcacgattg
    18541 cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc gggccaatgc
    18601 agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct gaatatatcg
    18661 ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg atttcaccgc
    18721 ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa ggttaattcc
    18781 gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc ggctgaagtt
    18841 ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac tttcttacgg
    18901 aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg ttcaagagaa
    18961 agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc tggcataaca
    19021 ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt tttcttagca
    19081 acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat tggataacga
    19141 tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc ctgctttgct
    19201 tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc gttttctaca
    19261 aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag tttcatcgct
    19321 tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt gaaagctggt
    19381 ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac ctcgattcca
    19441 tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc ttgttcaggt
    19501 atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat attcatgcct
    19561 cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa atcgaattcc
    19621 gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat atccaaaatt
    19681 attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct aatgaattac
    19741 tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc aagtcaatct
    19801 caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct aatgccagca
    19861 aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc atcgatttcg
    19921 tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt gcttttgctt
    19981 ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg acttgttccg
    20041 gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca gaaccatctg
    20101 gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg atttctccat
    20161 agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga acgcctgctc
    20221 cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca cgattaggat
    20281 cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc tcacaaatcg
    20341 ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg ttacggaatg
    20401 cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta caaatacctt
    20461 ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc attggtacga
    20521 acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg ttttcgattg
    20581 ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga ttcatttttt
    20641 ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca tagatcgaaa
    20701 acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg aaatctttga
    20761 cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc ggtgcgatgc
    20821 cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca ataggtgaag
    20881 tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt tcttatccgt
    20941 gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga ttttgtgcta
    21001 agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc ggaatttgct
    21061 tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt gtgatgaatc
    21121 ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc acagattttg
    21181 ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata tttccaatca
    21241 acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc atttttttca
    21301 atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat tttttgaaat
    21361 cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg tttactaaca
    21421 catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct ttctcataat
    21481 cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt tcttctatct
    21541 tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct tttgcaaaag
    21601 ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca ttttttcctt
    21661 ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg tctgcttatt
    21721 ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc ttaatacttt
    21781 tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat ggatactctc
    21841 atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg tttctttctt
    21901 cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga attgaatctg
    21961 acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat gaaaaggccc
    22021 gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta aaacagcttc
    22081 gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat aatttgcagg
    22141 agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt caatcggcgc
    22201 attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca tataagcgcc
    22261 tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag ctactaatgc
    22321 cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat gctccttcaa
    22381 taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt gcgtctgatc
    22441 caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa tctcacttgc
    22501 ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca taccttgata
    22561 ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca atatatccag
    22621 aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc aggaaatgtc
    22681 ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa gtcactaaat
    22741 cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca tcttcaattt
    22801 cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg atgtcagtag
    22861 tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct tgaatttttt
    22921 cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta taatttaaca
    22981 gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc tgtaaatatt
    23041 actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc caataagata
    23101 gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa cttttcttgc
    23161 ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg atgcaaaagc
    23221 agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac gtctcgtacg
    23281 gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc tgaatcttct
    23341 gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact tctggtaccg
    23401 tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa gagaatccca
    23461 cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat tagcttagat
    23521 agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat tttttcagcc
    23581 atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc gatagcatga
    23641 gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta atcgggcgac
    23701 attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg ttttatcaat
    23761 ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca tcttcgttca
    23821 cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt ggaaagatca
    23881 ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat tccaaattgt
    23941 tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt cagtgcatcc
    24001 agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac catttcacga
    24061 tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc caactttaca
    24121 acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac taacttgtta
    24181 atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc tgaagttgtt
    24241 tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga cagatctttg
    24301 aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa gacactaaca
    24361 agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact aattagtttg
    24421 attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg caaccttaaa
    24481 agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa acaactgata
    24541 aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat tttgattatc
    24601 aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt atttcaaaat
    24661 ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag aaaaaatgaa
    24721 aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta aggtcatcta
    24781 tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc agaataaaca
    24841 gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt tcgtcgtagt
    24901 agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt accgtccttt
    24961 ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta ccaataaggg
    25021 atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc tctataaaaa
    25081 aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt tgtctgaaat
    25141 tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt ttgaacagaa
    25201 aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga cttacttgaa
    25261 aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta tgcttcaaaa
    25321 agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc agtttttaag
    25381 aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga aaaagcacta
    25441 cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg tttctcctga
    25501 cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt caaaagaaga
    25561 aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag aacagtttga
    25621 aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc ttctcggctt
    25681 cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga ttaagtcata
    25741 gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag tatctgtttc
    25801 agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct ctacgccttc
    25861 tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag cctccagtcg
    25921 ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc ttatttctgt
    25981 tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa ctcgtttttc
    26041 atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa gcttgggttc
    26101 cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct tgggtagttg
    26161 ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa tctttgataa
    26221 aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga gaataattgc
    26281 ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc tttaaatcaa
    26341 gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta cctgcaatca
    26401 tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc aatgtaaatg
    26461 tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct tctccaccga
    26521 ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc ggtgcatcta
    26581 acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg cttggtgtac
    26641 ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc cccgactgtc
    26701 catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga atattacttt
    26761 gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact aaaggatcag
    26821 cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt agatggcgtg
    26881 cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt tcgacctcct
    26941 aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat tttttcataa
    27001 aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg ttcaatcgag
    27061 tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc ttgataaaat
    27121 tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc ttctaaaact
    27181 tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc aaagtcttcg
    27241 tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa ataagcattt
    27301 ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag cccttgtgtt
    27361 tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc tacatcattg
    27421 ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga gatcaattcc
    27481 tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt aggataagcg
    27541 gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata agaaatcctg
    27601 cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct taatttgctg
    27661 atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc agtaggaatc
    27721 acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg ttcacttgga
    27781 caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac atgggattgc
    27841 ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt atggtaagta
    27901 tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc tgctccgaat
    27961 tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata agcataccac
    28021 atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact tggcattcga
    28081 atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat atagacttca
    28141 tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac tccgctgact
    28201 tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc caaactagtc
    28261 cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta cactttcgta
    28321 tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg tttgataacc
    28381 ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc ggataaattc
    28441 ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat tatacactgg
    28501 aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa cgatcccttc
    28561 tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac cttctatgat
    28621 ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt ttacaatttt
    28681 ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa atgtgtattc
    28741 gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga aaaacaaacc
    28801 gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga acttttgttc
    28861 tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag cttgaatcgg
    28921 cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg tgatcagata
    28981 gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa gctggttcga
    29041 tccgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga caactttttt
    29101 tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg tattgatgtg
    29161 caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt ccgtcagagc
    29221 acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct tttcactttc
    29281 aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata acatagccta
    29341 caaactgtca ctcaaactta agccctattt ttgtctattc ttactgacaa aaacgttttc
    29401 ttaaaaaaga acaaaaaaac tggcgtagct cttttacaag ccacaccagc ttttgatacg
    29461 ttgtttattt tacatattca tgaacaagtt caacgacttc ttccatcata tcgcattctt
    29521 tcaatgcacg atcagcaagt tcagccattt tcgctgtgtc caagcgtttc attaaactac
    29581 gcgttttaag aatagatgtc gcactcattg agaactcatc tagacccata cctactagca
    29641 atggaacagc tgtttgatct ccagccattt ctccacacat acctgcccat tttccttcag
    29701 cgtgtgctgc atcaattaca tttttgatca aacgtaaaat tgatgggtta tatggttggt
    29761 acaagtaaga aacgcgttcg ttcatacggt ctgctgccat tgtgtattga atcaaatcgt
    29821 tcgtacctac agagaagaaa tctacttctt ttgcaaactt gtctgctaag actgctgctg
    29881 ctggaatctc gatcatgatg ccgacttgga tcgtatcaga tacttccttg ccttcactta
    29941 tcaatttttg tttttcttct tcaaagatcg cttttgctgc gcggaattct ttgagtgtag
    30001 ctaccatagg gaacatgata cgtaaattac catgaacaga tgcacgaagc aatgcacgca
    30061 tttgtgtacg gaacatttcg tcgccttgtt cagataaact gatacgcaat gcacgatatc
    30121 ctaagaatgg attcatttca tgaggtaatt gcagatatgg tagttcttta tctccaccga
    30181 tgtccattgt acgaacaact acaggttttc cttccatacc ttctaataca gctttgtaag
    30241 cttcatattg gtcatcttct gttgggaagt ctggagaatc catatacagg aattcagtac
    30301 ggtacagacc aactgcttct ccaccattat tatggacacc aactagatct tttggtgttc
    30361 cgatgtttgc agctaattca aaatgcttgc cgtcagctgt gacagtttct gcatttttta
    30421 atttttccca ttctgctttt tgtgctgcgt aatctgcacc tgctttttca aattcagctt
    30481 tttgttcatc tgtaggatcg atgattacat cgccttcgat cccgttgaca gccaaaatat
    30541 cgccttcttt gactttagca gtaatttctt ttgtcccaac gattgctgga atttctaaag
    30601 aacgtgccat gatagcagaa tgtgaagtac gtccaccaat gtctgttaca aacgctttta
    30661 caaaattgcg atctaattgt gctgtatcac ttggagtcaa gtcatgtgca acaacgacca
    30721 cttcttcgtt gatcattgat ggatttggta aagtaacgcc taataagtgc gctaaaatac
    30781 gtttagcaac gtcacgaata tctgcagcac gctcttgcat ataagcatta tcttccattg
    30841 cttcgaacat accgatatac atatccgtta cttcttttag cgctgattca gcatttacgc
    30901 tgttgtcttt aatattttgc ttgatttgac ctaccatttc tggatctgaa agaaccatca
    30961 aatgggcatc aaatacttgc gcttctgctt cacctaagct ttgcgctgct ttttcacgaa
    31021 tttgctgcaa ttcttcagta gatttagcta atgcagcatc caaacgagct tcttcagccg
    31081 cagtatcttc tactgttgcc ttactgaatg ttaaatccgg ttgaactagc aggtaagctt
    31141 ttgcaacggc tacaccgtca ctagcggcaa tcccttttag catttcaacc attattcaga
    31201 taagccttct ttcttcattg tttcaacgat tgctgccatt gcatcagctt cgtcagcacc
    31261 ttctgcagta atagtaacat cagaaccttg acctacacct aaagacataa cgcccatgat
    31321 tgatttcaag tttactgatt tacctttata ctctaagttt acatcagagt taaatttgct
    31381 tgctgtttgt actaatagtg tagctggacg agcgtggatc ccagtttctg ctactacgtg
    31441 aaattctttc ttttccatat taatcggtct cctttaagtg agtgttgttt ttatgtgagg
    31501 gttttctttt ttagtaattc agagaatcct aacaaatgcc accctttcat ctgtaaagat
    31561 taacattttt tttcgttaga tacaacagtt ttcattcaat ttcagataaa agtattcaaa
    31621 ttattccgga ttctttttca tccttttctt cacattttat aacaaacgtg tttatttcct
    31681 ttttcttgtc gctttctctt ctcctgacgt ttatcattct ttcctttgac cctctttgtt
    31741 ttcatatgaa acgaattact tgcttccctt tttattttta gtataataag agtcaaaggt
    31801 caggaatggt caatagagat ttcacctttt ctacctcata aatctcttaa aaaactaggg
    31861 gttattcttt tacctcatga aaaacgagat gtaaaatatg gatgtaccca tttttgtgaa
    31921 aggacgtgaa tgattcatgc tttgtcaaaa ctgcggcaag aatgaggcaa cgattcactt
    31981 gtatgccact gtcaatggtc agcggacaca actcgattac tgtcagagct gttatcaaaa
    32041 aatcaaaaat caacaaaatg gaggtctgac taatatggcc caaaatgatc catttggatt
    32101 tggcagtttg gacgatcttt tccgttccat gtctcgccaa atgcaacagc aaggaaatta
    32161 tgagcaaaca ccaccaacac aatttggcgg aaacaacaac ttcaatggtg gtcagccacc
    32221 tcaaggcaat acagaaggtt tattgggaga atacggaatc aatatcacag aacaagcacg
    32281 acaaggtgat atcgatcctg tgatcggacg cgatgacgaa atcaaacgag tcattgaaat
    32341 tttaaatcgc cgaaccaaga acaatcctgt cttgattgga gaaccaggtg ttggtaaaac
    32401 agcagttgtt gaaggtttag cacaaaaaat cgtagatggt gatgtaccgc aaaaattaat
    32461 ggacaaagaa gtcattcgtt tagatgtcgt atctttagtc caaggtactg gtatacgagg
    32521 acaatttgaa gaacgtatgc aaaaattgat tgaagagatc cgtcaagcag aaaatgtgat
    32581 cctattcata gatgaagttc acgaaatcgt cggtgctggt tctgctggtg atggaaatat
    32641 ggatgctggt aatattttaa aaccagcttt agctcgagga gaattgcaaa tggttggtgc
    32701 tactacctta aatgagtacc gcatcatcga aaaagacgct gctttagaac gtcgtatgca
    32761 acctgtacgg gtagatgaac caactgtcga agaaacgatc agtattttga aaggtttgca
    32821 aaaacgttat gaagactatc atcatgtaaa atatactgat gaggccatcg aagcagccgc
    32881 aactttatcc aatcgttacg tccaagatcg cttcttgccg gataaagcca tcgatttgct
    32941 agatgaaaca ggttctaaga aaaacttgac gatccaaatc gttgatccta aaacaatcga
    33001 gaaaaaactg caagaagcag aagaacaaaa agttttagct tctcgagaag aagatttcga
    33061 aaaagcagct tattatcgcg atcagatcaa taaacttcaa aaaatgaaag aacgtcagct
    33121 cactgaagag gaaacacctg tgattaccga aaaagatatg gaaaaaatcg ttgaacaacg
    33181 gacaggtatt ccagtgggtg aattaaaaga aaaagaacag acacaactga aaaacttagc
    33241 agatgacttg aaagctcatg tcatcggaca agataacgct gtagatcgag tagcgaaagc
    33301 gattcgccga aaccgtgttg gattaaacaa acagaatcgt ccaatcggct ccttcttatt
    33361 tgttggacca actggtgtag gtaaaacaga gcttgcgaaa caacttgcct acgaactatt
    33421 cggttcacaa gattcaatga ttcgtttcga tatgtctgaa tatatggaaa aacatagtgt
    33481 ctctaaattg attggttctc ctccaggtta tgtcggatac gaagaagctg gtcaattaac
    33541 agaaaaagta cggagaaatc cttacagtct tgtcttgtta gatgaagtag aaaaagctca
    33601 tccagatgtt ttgcatatgt tcttgcaaat cttagatgat ggtcgattga cagatgcaca
    33661 aggaagaacg gtcagcttta aagatacgat catcattatg acaagtaatg ccggaactgg
    33721 taaagtagaa gcaaacgttg gttttggtgc ggcacgcgaa ggtgttactc gttctgtatt
    33781 gaaccaatta aacaactact ttacaccaga atttctgaac cgttttgacg ggatcatcga
    33841 attcagtgca ttatcaaaag aaaacttgat gacaatcgtc acactaatgc tagacgatgt
    33901 caaccaaatg ctggcagcac agcaacttca catcgaagtt cctacgaatg tcaaagaaaa
    33961 attagttgac ttaggctatg atccatctat gggtgctcgt cctcttcgac gcacgatcca
    34021 agaacaaatc gaagatggta tcgctgaatt ttatcttgat cacccttcta tccatgagtt
    34081 gaaagcaaaa ctggataaag atggaaaaat catcgttact tctaaaccag aacgtttggc
    34141 taaagaatca gccgaagaaa cggctgaata aatccagttt tatagcgtga gacggtattt
    34201 ctttgagaaa tgccgtctca ttcttttttc agaaaagtca aaatactgtt cattttataa
    34261 aaaagaaatt ataataagag aaacaagcaa ttaaaaatac ataaaagagg tgagcgccat
    34321 gaaattggta aatgtaacaa acagccattc ccgtcttgta ttaaaccagc ttgaaaacac
    34381 tgatgcccat ctagtcaagg tctacacagc cggaaatacc actattgtct acactgaagc
    34441 ccctgaacat aatgaaattc tgctgatcaa tgacaaacgt aaaatccagc cgaaagaaat
    34501 cgaagatgct aaaaattatt tcttagataa aatcaaagac gccgtttacc acgaggatga
    34561 aatcaaagta atcgaattgg caggccttgt tgaaatctcg attccaaaac atgaattagg
    34621 cgctacatta gccgtgtaaa caaattttga acataataat taaagagcgt gggacaaaag
    34681 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
    34741 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
    34801 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
    34861 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
    34921 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
    34981 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
    35041 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
    35101 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
    35161 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
    35221 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
    35281 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
    35341 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
    35401 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
    35461 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
    35521 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
    35581 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
    35641 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
    35701 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
    35761 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
    35821 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
    35881 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
    35941 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
    36001 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
    36061 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
    36121 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
    36181 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
    36241 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
    36301 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
    36361 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
    36421 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
    36481 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
    36541 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
    36601 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
    36661 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
    36721 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
    36781 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
    36841 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
    36901 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
    36961 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
    37021 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
    37081 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
    37141 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
    37201 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
    37261 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
    37321 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
    37381 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
    37441 ctaatgccgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
    37501 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
    37561 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
    37621 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
    37681 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
    37741 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
    37801 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
    37861 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
    37921 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
    37981 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
    38041 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
    38101 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
    38161 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
    38221 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
    38281 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
    38341 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
    38401 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
    38461 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
    38521 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
    38581 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
    38641 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
    38701 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
    38761 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
    38821 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
    38881 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
    38941 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
    39001 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
    39061 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
    39121 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
    39181 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
    39241 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
    39301 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
    39361 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
    39421 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
    39481 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
    39541 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
    39601 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
    39661 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
    39721 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
    39781 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
    39841 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
    39901 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
    39961 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
    40021 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
    40081 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
    40141 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
    40201 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
    40261 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
    40321 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
    40381 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
    40441 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
    40501 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
    40561 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
    40621 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
    40681 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
    40741 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
    40801 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
    40861 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
    40921 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
    40981 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
    41041 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
    41101 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
    41161 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
    41221 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
    41281 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
    41341 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
    41401 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
    41461 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
    41521 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
    41581 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
    41641 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
    41701 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
    41761 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
    41821 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
    41881 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
    41941 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
    42001 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
    42061 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
    42121 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
    42181 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
    42241 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
    42301 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
    42361 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
    42421 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
    42481 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
    42541 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
    42601 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
    42661 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
    42721 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
    42781 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
    42841 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
    42901 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
    42961 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
    43021 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
    43081 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
    43141 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
    43201 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
    43261 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
    43321 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
    43381 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
    43441 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
    43501 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
    43561 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
    43621 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
    43681 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
    43741 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
    43801 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
    43861 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
    43921 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
    43981 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
    44041 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
    44101 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
    44161 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
    44221 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
    44281 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
    44341 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
    44401 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
    44461 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
    44521 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
    44581 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
    44641 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    44701 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    44761 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    44821 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    44881 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    44941 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    45001 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    45061 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    45121 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    45181 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    45241 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    45301 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    45361 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    45421 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    45481 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    45541 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    45601 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    45661 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    45721 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    45781 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    45841 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    45901 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    45961 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    46021 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    46081 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    46141 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    46201 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    46261 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    46321 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    46381 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    46441 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    46501 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    46561 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    46621 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    46681 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    46741 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    46801 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    46861 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    46921 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    46981 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    47041 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    47101 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    47161 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    47221 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    47281 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    47341 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    47401 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    47461 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    47521 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    47581 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    47641 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    47701 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    47761 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    47821 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    47881 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    47941 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    48001 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    48061 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    48121 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    48181 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    48241 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    48301 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    48361 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    48421 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    48481 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    48541 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    48601 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    48661 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    48721 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    48781 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    48841 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    48901 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    48961 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    49021 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    49081 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    49141 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    49201 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    49261 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    49321 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    49381 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    49441 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    49501 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    49561 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    49621 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    49681 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    49741 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    49801 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    49861 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    49921 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    49981 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    50041 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    50101 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    50161 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    50221 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    50281 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    50341 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    50401 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    50461 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    50521 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    50581 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    50641 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    50701 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    50761 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    50821 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    50881 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    50941 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    51001 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    51061 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    51121 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    51181 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    51241 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    51301 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    51361 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    51421 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    51481 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    51541 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    51601 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    51661 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    51721 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    51781 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    51841 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    51901 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    51961 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    52021 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    52081 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    52141 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    52201 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    52261 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    52321 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    52381 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    52441 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    52501 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    52561 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    52621 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    52681 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    52741 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    52801 a
//
LOCUS       DACEKH010000008        51804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEKH010000008 DACEKH010000000
VERSION     DACEKH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.008"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..100)
                     /locus_tag="IW662_000410"
     CDS             complement(<1..100)
                     /locus_tag="IW662_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAQ0865261.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVF"
     gene            complement(115..1362)
                     /locus_tag="IW662_000411"
     CDS             complement(115..1362)
                     /locus_tag="IW662_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0865262.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(1438..1584)
                     /locus_tag="IW662_000412"
     CDS             complement(1438..1584)
                     /locus_tag="IW662_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ0865263.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(1688..1999)
                     /locus_tag="IW662_000413"
     CDS             complement(1688..1999)
                     /locus_tag="IW662_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0865264.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(2001..2198)
                     /locus_tag="IW662_000414"
     CDS             complement(2001..2198)
                     /locus_tag="IW662_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ0865265.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(2592..4319)
                     /locus_tag="IW662_000415"
     CDS             complement(2592..4319)
                     /locus_tag="IW662_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0865266.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(4312..5505)
                     /locus_tag="IW662_000416"
     CDS             complement(4312..5505)
                     /locus_tag="IW662_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0865267.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(5692..6336)
                     /locus_tag="IW662_000417"
     CDS             complement(5692..6336)
                     /locus_tag="IW662_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0865268.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(6430..6564)
                     /locus_tag="IW662_000418"
     CDS             complement(6430..6564)
                     /locus_tag="IW662_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ0865269.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(6566..8698)
                     /gene="feoB"
                     /locus_tag="IW662_000419"
     CDS             complement(6566..8698)
                     /gene="feoB"
                     /locus_tag="IW662_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ0865270.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(8695..9168)
                     /locus_tag="IW662_000420"
     CDS             complement(8695..9168)
                     /locus_tag="IW662_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ0865271.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            9569..9793
                     /gene="nrdH"
                     /locus_tag="IW662_000421"
     CDS             9569..9793
                     /gene="nrdH"
                     /locus_tag="IW662_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ0865272.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            9952..12111
                     /gene="nrdE"
                     /locus_tag="IW662_000422"
     CDS             9952..12111
                     /gene="nrdE"
                     /locus_tag="IW662_000422"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ0865273.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKAIYYVRTFTDDAEEIGSNQCESCVI"
     gene            12161..13126
                     /gene="nrdF"
                     /locus_tag="IW662_000423"
     CDS             12161..13126
                     /gene="nrdF"
                     /locus_tag="IW662_000423"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ0865274.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(13215..14354)
                     /locus_tag="IW662_000424"
     CDS             complement(13215..14354)
                     /locus_tag="IW662_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0865275.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(14663..15376)
                     /locus_tag="IW662_000425"
     CDS             complement(14663..15376)
                     /locus_tag="IW662_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ0865276.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(15630..16331)
                     /gene="nagB"
                     /locus_tag="IW662_000426"
     CDS             complement(15630..16331)
                     /gene="nagB"
                     /locus_tag="IW662_000426"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ0865277.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            16565..18097
                     /locus_tag="IW662_000427"
     CDS             16565..18097
                     /locus_tag="IW662_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0865278.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(18288..19490)
                     /locus_tag="IW662_000428"
     CDS             complement(18288..19490)
                     /locus_tag="IW662_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0865279.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(19572..20261)
                     /locus_tag="IW662_000429"
     CDS             complement(19572..20261)
                     /locus_tag="IW662_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0865280.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(20367..20735)
                     /gene="rplL"
                     /locus_tag="IW662_000430"
     CDS             complement(20367..20735)
                     /gene="rplL"
                     /locus_tag="IW662_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ0865281.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(20788..21291)
                     /locus_tag="IW662_000431"
     CDS             complement(20788..21291)
                     /locus_tag="IW662_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ0865282.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(21701..22390)
                     /gene="rplA"
                     /locus_tag="IW662_000432"
     CDS             complement(21701..22390)
                     /gene="rplA"
                     /locus_tag="IW662_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ0865283.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(22494..22916)
                     /gene="rplK"
                     /locus_tag="IW662_000433"
     CDS             complement(22494..22916)
                     /gene="rplK"
                     /locus_tag="IW662_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ0865284.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            23259..23927
                     /gene="sdaAB"
                     /locus_tag="IW662_000434"
     CDS             23259..23927
                     /gene="sdaAB"
                     /locus_tag="IW662_000434"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ0865285.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            23940..24812
                     /gene="sdaAA"
                     /locus_tag="IW662_000435"
     CDS             23940..24812
                     /gene="sdaAA"
                     /locus_tag="IW662_000435"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ0865286.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(24903..25922)
                     /locus_tag="IW662_000436"
     CDS             complement(24903..25922)
                     /locus_tag="IW662_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0865287.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(26095..26982)
                     /locus_tag="IW662_000437"
     CDS             complement(26095..26982)
                     /locus_tag="IW662_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0865288.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(27059..27604)
                     /gene="nusG"
                     /locus_tag="IW662_000438"
     CDS             complement(27059..27604)
                     /gene="nusG"
                     /locus_tag="IW662_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ0865289.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(27705..27875)
                     /gene="secE"
                     /locus_tag="IW662_000439"
     CDS             complement(27705..27875)
                     /gene="secE"
                     /locus_tag="IW662_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ0865290.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(27899..28051)
                     /gene="rpmG"
                     /locus_tag="IW662_000440"
     CDS             complement(27899..28051)
                     /gene="rpmG"
                     /locus_tag="IW662_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0865291.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            28213..28857
                     /locus_tag="IW662_000441"
     CDS             28213..28857
                     /locus_tag="IW662_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0865292.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(28913..29416)
                     /locus_tag="IW662_000442"
     CDS             complement(28913..29416)
                     /locus_tag="IW662_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0865293.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(29429..29475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            30013..30978
                     /locus_tag="IW662_000443"
     CDS             30013..30978
                     /locus_tag="IW662_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0865294.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(31077..32249)
                     /locus_tag="IW662_000444"
     CDS             complement(31077..32249)
                     /locus_tag="IW662_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0865295.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(32236..33381)
                     /locus_tag="IW662_000445"
     CDS             complement(32236..33381)
                     /locus_tag="IW662_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0865296.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(33395..34319)
                     /locus_tag="IW662_000446"
                     /pseudo
     CDS             complement(33395..34319)
                     /locus_tag="IW662_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            34436..35515
                     /locus_tag="IW662_000447"
     CDS             34436..35515
                     /locus_tag="IW662_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0865297.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            35547..36140
                     /locus_tag="IW662_000448"
     CDS             35547..36140
                     /locus_tag="IW662_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0865298.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(36250..36477)
                     /locus_tag="IW662_000449"
     CDS             complement(36250..36477)
                     /locus_tag="IW662_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0865299.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(36580..39168)
                     /locus_tag="IW662_000450"
     CDS             complement(36580..39168)
                     /locus_tag="IW662_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ0865300.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(39188..39847)
                     /locus_tag="IW662_000451"
     CDS             complement(39188..39847)
                     /locus_tag="IW662_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0865301.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(39896..40399)
                     /gene="trmL"
                     /locus_tag="IW662_000452"
     CDS             complement(39896..40399)
                     /gene="trmL"
                     /locus_tag="IW662_000452"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ0865302.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(40620..41486)
                     /locus_tag="IW662_000453"
     CDS             complement(40620..41486)
                     /locus_tag="IW662_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0865303.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            41595..42236
                     /locus_tag="IW662_000454"
     CDS             41595..42236
                     /locus_tag="IW662_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ0865304.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(42298..43659)
                     /gene="mgtE"
                     /locus_tag="IW662_000455"
     CDS             complement(42298..43659)
                     /gene="mgtE"
                     /locus_tag="IW662_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ0865305.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(43677..44576)
                     /locus_tag="IW662_000456"
     CDS             complement(43677..44576)
                     /locus_tag="IW662_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0865306.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(44578..45375)
                     /locus_tag="IW662_000457"
     CDS             complement(44578..45375)
                     /locus_tag="IW662_000457"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ0865307.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(45353..46057)
                     /locus_tag="IW662_000458"
     CDS             complement(45353..46057)
                     /locus_tag="IW662_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ0865308.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            46188..46763
                     /locus_tag="IW662_000459"
     CDS             46188..46763
                     /locus_tag="IW662_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ0865309.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            46881..47558
                     /locus_tag="IW662_000460"
     CDS             46881..47558
                     /locus_tag="IW662_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0865310.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(47637..49445)
                     /gene="pepF"
                     /locus_tag="IW662_000461"
     CDS             complement(47637..49445)
                     /gene="pepF"
                     /locus_tag="IW662_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ0865311.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(49621..50619)
                     /locus_tag="IW662_000462"
     CDS             complement(49621..50619)
                     /locus_tag="IW662_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ0865312.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(50845..51498)
                     /locus_tag="IW662_000463"
     CDS             complement(50845..51498)
                     /locus_tag="IW662_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ0865313.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        15618 a        10778 c         8971 g        16437 t
ORIGIN      
        1 taaatacttt atcgtgaaaa agaatgaaat tatcaataat tcgttctaac tgatttaatt
       61 gtataatttg atcttcacat aatattattg gataactcat aaatcctcct tccctcaaaa
      121 caacaaaata acttgagtag taaattgata cggatcgttt tgatagttca caaacatatt
      181 gggattcttt ttattgattt cctctatact agataaacct aacccttgat gtcccttttt
      241 tgttgttatt ccttttttca ttagagtatc tatagagagt agtatctctc tacaactatt
      301 cgatatcgaa atttctatct gtttatcatc tcggtaaata gctagagaca gttctctatc
      361 ttgactctct atagatgctt caattgcatt atcgagaata attccaagca ccctaataca
      421 gtcaaatatt ggaacaggaa cttcaataat agtacttgga cattcgaatt tacaacaaat
      481 cttattgtta tttgcttggt atagcttcgc tatcaacaaa cttttcaaat attcattttt
      541 tatatttttt aaataacgat attcaaactt attggttaga taagaattag aatagatctc
      601 taattctttt atttcttttt ccaatatcga atcatggttt tgattagcga tttcttttaa
      661 actcagcaat aaatttttat agtcatgtct aaatctggca aatttttctt gatctttttc
      721 cagtgaatca gtatattttt ttagataaac caattctttt cttttcaatt gctgttcata
      781 ttgttctcgt tgcctaatag ttattctaat aaataaaaat agaacaacaa acatttgaat
      841 gattaaaaat attaagacac caaacacaaa atgatcaaat acttgatatt gatgagcaac
      901 ataagaaaca aataaaccaa ttgtaagtaa ataaatcatg aatatagcag taagtttaga
      961 actggactgt tcaacaatcc tatttatagc taattttcta tataaccaaa taaacatcag
     1021 caaaaacaaa gaatcaagca ttatctggaa tactacataa ccataccctt ttatcccttc
     1081 aaatgaaaac atatacgtca taaccatgga agtaataata gatctgaaat catcaataag
     1141 catacataat agtaatttat ttaaaagcac tatattagtt ttcttctttt cctttaatat
     1201 aaaataactt ccgattatag gtatagctgc ggaaacatca gtaaataatt caacaaaaaa
     1261 agtgagtggt atcaataaac atacaaaaag atattttctt ttgttaagat cagtattagt
     1321 catataaaat aagagaaaaa agaaaaagtt tcccacgaac attcttaata gataagcttt
     1381 tgtatcgata gtaatcaaga gatcccctcc tgattttagg atatcataaa aaaaatttta
     1441 gttacacttt ttaaataatg aaaaaacaca ttccactaaa tttgatgccg gttttccttg
     1501 aggttttgtt ccagcacctc ctttaatttt tttaagctct tgatctgaac attgtttagc
     1561 atttagtcta tttttttctt ccattacgat tccccctaca aactataaat acttttatgt
     1621 cactgattca atatacctat ttttctaatc aaaaaatttt gagacatatt taatattata
     1681 agtatcatta aaattgagat ttatctccat aatctgctcg atataaacca tttattccag
     1741 cagttcttcc aatttcactc aaatttctta ttaattcctc ttgatattca gtgaatctta
     1801 ttttattagt ataagcagta aaatatagat aattcaccaa tctatttatt aaaggaccgg
     1861 gatctaattc atatttttgt ttttctaatt ttaaatatac cttttgtaaa acctcaagaa
     1921 tatcagaata tttaggatct ttacttatag atatatcatt atataattca tgaacaattt
     1981 gcttagcatt ttttttcatt ttagcacttc cctggaattg ctccacctaa aaaaccacct
     2041 atagacattc ctgcaataca agtagttgcc ttggcccaat cgaccgtaca tttattttta
     2101 gtgcaataca ctccatttcc ataatatttt ccactatgag tggtaccccc atagataagt
     2161 tgtgtctctt taatagacaa aatttttaaa tgtttcataa tctaatcacc cctaaattaa
     2221 tgtttttaga ctattattct aacattcatt aaatttcaaa aaacaattcc ttcttgaaac
     2281 gtagcaaaat aattcctgaa taagaattta atcactttta agtatataaa ctatgtatat
     2341 tctttaatat tttttgagaa tgcacttcgt acataataaa ctttaatcac attagtcttt
     2401 catacctacg atcgatctct ttgactcgca cccataaatt cataatagtt tgtcggcgtc
     2461 tttggacgtt tcgaagatga gctacgcatt ctcttttttt atagacaaaa aagatagaga
     2521 cgaacatgga gcttccaaat ttggatattt gatccaattt tggaagctcc atttatttcg
     2581 tgtctgtctt tttatttttt ccagctttcg actaattctt ggttctcttt gatccagtct
     2641 tttgctgctt gctgtggatc ttttccattg ttgatttcca acataacttt ttccatatct
     2701 ttttctgacc aatggaaatt gtctaatact ttgtaagctt ctggctggtc ttctttcaat
     2761 ccttttcgaa ccatcgtatg gatctgctct tcgcttccca ttccgttttc aggatcttct
     2821 aagtatttca gatcatattt ggcaaacatc caatgaggtg tccatcctgt aactacaatt
     2881 ggttcgtgtt ttttgatcgc ttgacctagt gcaacggtca ttgctcctga agaagatgtc
     2941 tctactttcc agtctttcaa gttgtcatat ttttggatcg tgttttcagc agccgtaact
     3001 acaccagctc ctggctcgat tcctatgatt ttcttacctg cctgatcggt taactcatcg
     3061 atcgaattta catccatgta ggctggcact gctaaaccaa cttttgctcc tgtcaaattg
     3121 gcacctaggt cttcgacttg atctttatat tgtgcatatt gtgaaccatg agttttaggt
     3181 agccaagctg aaaccatggc atctgactcg ccatttgaaa cagatttcca catgattgaa
     3241 ttatctaatg gcgtcatcgt tacatcatag cccatttgtt tcaatacttc accgactaca
     3301 tttgttgaag caacttctgt atcccattca acataagata agttgatacg ttttgtttct
     3361 ttggcagatg aaaaactccc tataccaatc gcacctaaga tcactactac tacagcacca
     3421 atgatcagtc cttgacgttt tttacttttc tgtgttgttg ctcctgcagc ttttttattg
     3481 tttggctggt tcaaatgctg tgtaaaacgg tcaatgatga ttgctaagat aactaatgca
     3541 accccgttga caaatccatt cccgacttgt gctctttgca atgctgataa tacgccgcgt
     3601 cctaagccgg gtgcaccgat catagaggca ataacgacca ttgaaagtga cagcattgtt
     3661 gtttgattga tccctgccat gatcgtactt ttagctaaag gcaattctag tttgaatagt
     3721 ttttgacgac ttgtgctccc gaatgaatca gaagcttcta ctaattcttt aggaacttgt
     3781 cgaatcccta aattcgtaaa acgaacggtt ggtggcagag caaagatgac tgaggcaaat
     3841 acccctggaa ccatcccgat accgaaaaat gcaactgctg gaatcaaata aacgaatcct
     3901 ggcattgtct gcataaagtc aaggatcggt ttgataatac tttgggcttt actgcttttt
     3961 gccatcaaga tccctaatgg taccccaatg atgatcgaga tcacactaga taaaagaaca
     4021 agtgtgacag tattcatcaa atcattccat agaccttggt tataaataaa ccatagaccg
     4081 attaatgtga acaacgtaag tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc
     4141 aataacaaga taaacaaagg agctggaatc actaacagta aatttgtaat ccccgacatc
     4201 actgcctgtc caatcgtttg taaaaatgaa aataggccag aaaaagttgt cgtaaaccat
     4261 tctgtgatat tttctaccca gtttgccact ggtaattgat agttcaatag attattcatg
     4321 ttcactcacc tcgcttgttt ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc
     4381 ttttaatttt ccatcctcta caactgcaag tggagtcggt gagtcataaa taatattgaa
     4441 aatatcactt accaccatgt ctttcccaat caccgtcaca ttttcatcta ggaagtcgat
     4501 caatggacgt ttttgcttcc tagcttctaa cgcttgatct gcagtgatga tccctttcag
     4561 gtgtctcttt ctgtcaacag ccatcagcat gctgacttct tcattacgca tccttgtcaa
     4621 tgcaacgttt ggtccatcag attcgatatt cgtcgtcaat gcaggtacca tgatgttttg
     4681 agccgtaagc actttggaac gatcgacttc ttccacaaat tcacgtacat aatcatttgc
     4741 tggattggtc aatatttctt cacctgttcc gatctgcatg atttcgccgt ctttcatcaa
     4801 ggcgattctg tccccaatcc gtaaagcttc attcaaatca tgggtaatga aaataatcgt
     4861 tttttgaaca ttggcttgca ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg
     4921 atcaagagcc gagaacgctt cgtccatcag caagatctca ggatcatttg ctaaagctct
     4981 ggctaggcca acccgttgtt gcatcccacc agataactga ctaggatatt ggtctttaaa
     5041 tgaaagcaaa ctagaatttt ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg
     5101 tacaccacgg acttctaacc cgtattctgt attttccaag atcgttcgat gtggaaacag
     5161 accgaagttt tggaaaacca tattgatttt atgacgacgg acttctcgca attcttcttt
     5221 gcttaacttc gatacatctt ctccatcaat atagatactg cccgaagttg gctcaatcag
     5281 acgattcaat agacggatca atgtggattt cccactcccg gaaagcccca tgatcacgaa
     5341 gatttctcct tcttttacgt caaaattcac gtcatacaca ccaaccgttg caccggtttt
     5401 ctttaaaatc tccgttttcg gctggtgatc attcatcata tctaaagcgg cttgtgtctt
     5461 tttaccaaat atctttgtta aatgttttac ttctactttt accaaacaaa ttctcctttt
     5521 ttatgtaaat tcatttttct ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt
     5581 cactttgtca aaaatgaaag aaatttgctt gtccaaactg aaaaaaactt ggctaaatct
     5641 ttgtaaataa aaggtttagc caagttaata tttttttaat atttcacaca atcattctgc
     5701 aagctgatgc tccgggtaaa aaaagtttgt catacgctgt agtgtatatt catttccaat
     5761 aaaatgaacg atgtcccctg ccgtaaattt agcatagggg ccaggtgaaa caagcagttc
     5821 ttcattatga gaaattgcga taactgtggc gccagtcgcc tgccagatat tcaactcgct
     5881 aattgaacgg tccagatgca ttgctttttc tgataaagat aattcaaacg gaagtaaagg
     5941 attcatctta tgtattcgct tcgtttgatt gaccagctga tccaataatt gagaaaaatg
     6001 attcagctca tcttgttgct tctccacact tgccatgatt tctgcttttg tatcttggat
     6061 ggattgtaca tcttgatatt gcaataggaa atcttttgct ttttccttcg aagcaacaaa
     6121 tgccccactt ccatgccgta cttccatgat tccaagatct accagcacat tgatagcctt
     6181 gcgggtagtc tcaggagaag cattgtacgt actggcaagt gttgagcggg catggatctt
     6241 ctcacccact ttatatcggt tttcaacgat acgttcggct aaatcaacag caatctgctg
     6301 gtagcgcggg cggacgattt ttttctttcc agacattttc tttcctcttt ctttttttac
     6361 tttccctcat cttactatgc tctagcaaaa taagcaaaaa aggggagaat agcctcttgc
     6421 aacgactgat catcgcttga tctgttcctt ttttactggg caagctgttt gacagtcatc
     6481 acaattctta ccggatttta aacgagtata gacaattgcg cctgcacttc caaagattaa
     6541 cacacttaat aagaatgtcg ccatcctatt cctctccttc taatgacaat atagtataaa
     6601 actggttctt ggattttggc tgacggaaca aaacataaac gagtcctatc atcagtaaaa
     6661 tagcaagata cgtccctaac gctacctgtc caccctccaa taagtgtccc aactgataaa
     6721 tcacaaaact aactaaataa gctaaaccgc attgatagcc tatggcgatc caagtccatt
     6781 tcttggcatt catttcccga tggatagccc caattgccgc aaaacaaggc gcacaaagta
     6841 aattgaaggc taacaatgaa taagctgctg cgggagtata tgtgtgttgt agcacttgcc
     6901 agatttctct gccattttcg gatacttccg catgaccaaa caaaatacca aatgtgccaa
     6961 taatattttc tttagcgacc aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc
     7021 aaccaagcgg ctgaaaaaca actgccagga ttcgcccgaa aaaggctaag atactttggt
     7081 cttcagaaac aggttgagca taaaaattat aagaagagct gaaccagatc acgatactag
     7141 aaacaaagat aatcgttcct gcttttttaa caaaagactt gctgcggtca taggtttgtc
     7201 ttaggaccgc tgaccattga ggaaaatgat atgcgggcaa ttccatgata aaaggagctg
     7261 tatctccagc aaacaaacgt gtttttttca agctgattcc agagaaaata attgcagtca
     7321 taccaagaaa ataggcagaa ggagctaccc aagattgatg aggaaaaaag gcccctgcta
     7381 ctaaggcaat aatcggcaac tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca
     7441 aacgacgatc ttgctcgttt tcaatcgtgc gactggccat gacgcctggc actccacatc
     7501 ctgtagcaat cagcatagga atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc
     7561 gatccatgac aaaagcgatc ctcgccatat atccgcagtc ttccagaaag ctcaagcata
     7621 aaaacaatat aatcaattgc ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc
     7681 cgttcaagat cagctcctgc atccatcccg ctacttgcca ttcagctaac aatctgccaa
     7741 catgttctgg taaccatgta ccaaacaatg tgtcattgat ccaatctgtc cccattgttc
     7801 ctactgtttg aatagccaga taatagatca gccacatcac aaatgcgaag atcggcaagg
     7861 ctaaccaacg attcgttgca atctgatcga ttttgtcact catcgataat tggaaagaat
     7921 catttgacac aacacagagt gtacatagtt gggtaataaa ttcataacgt tcattgacca
     7981 gaatcgtctc actatcgtcg tccaatagtt tctctgttaa ctggatcagt tgctcgatct
     8041 ctttttgctg agaaactgct atctcgtaat cctctcttac cttctgatct cgttcaaaat
     8101 atttcattgc ataccagcgt ttcttgccgc tttgttctcc tagtacatct tcaatttcat
     8161 gtaaagctgc ttctaaacgt ggatcatatg tacagggaac aggaatagaa ggccgctttt
     8221 ttgcagactc ctgaatcttt tcgattagtt tttcgattcc ttgctttttc aatacgctga
     8281 ttggtaaaac tggtacacca agaccatagg aaagcttttc tagattgatt ttttttcctt
     8341 cttttttgat gacatccatc atattcagac caatgatcat aggaatgtct aaatccaata
     8401 actgcgtagt taaataaaga tttcgttcta aattggtagc atcaataatg ttgatgatca
     8461 gttcaggctg ttcctgaacc aaataatctc ttgcaatcac ttcttctggt gtataaggag
     8521 acaaagaata gatgccgggt aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc
     8581 cttctttttt ttctactgtt acgcccggcc aattccctac atattgattc gaaccagtca
     8641 agagattgaa caagctagtt ttcccgctgt ttggatttcc gactaacgcg atattcattc
     8701 tttttcctcc ctttgtatca agattagttc ggcctctgac tttcgtaacg tcagttcata
     8761 ccctcgcact gtcagttcga ttggatctcc cagaggggct agttttcgga taactatttt
     8821 tacatttcgc gtcaatccca tatccattaa ccgtcgcttc actgcgcctt gtccgtggat
     8881 tgctacgacg atcccagtct ctcctgcttt taattgatca agagaagtcc agctttcaga
     8941 ggaagcagtc tttttattga caaagatttg acctagtatt tcccgactta ctgccactcg
     9001 atgacttttt accagtaaaa tggcattgtt gcctgataaa ttgacgacga cgattttact
     9061 tccggcaacg atcccgatat tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg
     9121 ttcgacttga taaacgaaat tctgctttgc ctgcgctaag gtctgcatat tgattcctct
     9181 cttgtgaaat aaatcatgta ttaaaatcat tcattatatg ttctaatttt cttacgtgtt
     9241 acgcaaataa atgagaatca ttctcacata gaatactctc ggtaattgag aatgtcaatt
     9301 atacttgtga tattatgagc gaatctttag tattgataga taaaaatgag aaaaaaatat
     9361 ttcttcatag ttttccacaa ttttttctct attaaatttg ctattttccc acacgatact
     9421 tgttttcagc tcttttttca aaaacacaaa acttagtatt ccacagtttc cacaacacaa
     9481 tatgtagtgt ttgttgtttg acttttttac taagagtttt atactatcta ttatagataa
     9541 taagaatgtg ttagaggagg cactcaccat gaacgtaaaa atcttttcta aaaataattg
     9601 tatgcaatgt aagatggtca aacggttttt aacagaaaat caaattgctt ttgaagaagt
     9661 caatatcgat gaacagcctg aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt
     9721 tcctgtcatt acttctgatg cagaaacagt tatcggattc cgtcctgatc agttgcgtaa
     9781 acttgccagc taacatgact aaaagaatca aaacgtgata catggatttg aagggcttga
     9841 gacagcacca gtttttgtcg caaacttcca aatccttttt taaagcttaa aaacaatgca
     9901 atgatttgca atcaagcatc atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt
     9961 aaaaatttac aagatgtcag ttatttcaag ctgaataatg aaatcaatcg gccagtcaat
    10021 ggtcaaatcc ctttgaataa ggataaagaa gctttagttg ccttttttga ggaaaatgtc
    10081 aaaccaaata cgatgacttt tctatctatt atggaaaaaa tcaattactt gatcgatcaa
    10141 gaatatatag aagcagaatt cattcaattg tattcaccat catttattga aaagctatat
    10201 caatttttgg acgagcaaca tttttctttc aaatctttta tggctgctta caaattttat
    10261 tctcaatatg cgttgaaaac caacgacggt tccgcttatc tggaaactta tgaagatcga
    10321 gtagccttca atgcgcttta ctttgcgaac ggggatgaag aattagcact tgctcttgca
    10381 gacgaaatga tccatcaacg atatcagcca gctacacctt ctttcttgaa tgctggacgt
    10441 aaacgtcggg gcgaattggt gtcttgtttc ttgactcaag tcactgacga tatgaactct
    10501 atcgggcgct caatcaactc tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt
    10561 actttatcaa atcttcgtga agccggcgct cctatcaaag gatatgaggg tgctgcaagt
    10621 ggtgtcgttc cagtcatgaa attattcgaa gatagtttca gctattctaa tcaattaggt
    10681 cagcgtcaag gagctggcgt ggtttatttg aacgttttcc atccagacat tattgctttc
    10741 ctttcagcaa aaaaagaaaa tgcagatgaa aagattcgcg taaaaactct ttctcttggc
    10801 gtgatcgtac ctgataaatt ttatgaattg acacgtaaaa acgaagatat gtatttattt
    10861 agtccatata gtgtcgaaaa agaatatggt gtcccattct cttatgtaaa tattacagct
    10921 gagtatgatc atctagttgc aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat
    10981 cttgaaaatg aaatctcaaa attacaacaa gaatctggct atccttacgt aatcaatatt
    11041 gatacagcga accgcgagaa tccaatcgat ggcaagatca tcatgagtaa tttatgttca
    11101 gagattttac aagttcaaac gccttctgtg attaacggca aacaggaata tgaagtatta
    11161 ggaacggata tctcatgtaa cttaggctcg acaaatatcg tgaatttgat gcaaagccct
    11221 aactttggtc aatctgtgcg tacgatgact cgtgcattga catttattac agatgcatct
    11281 gatatcgatg tcgtaccacc gatccaaaac ggaaataagc tgaatcatac gattggatta
    11341 ggtgccatgg gcttacacac ttatcttgca aaagaacaaa tggaatatgg ctcagaggat
    11401 tcattagact ttacaaatat ttacttcatg ctgttgaatt attggacact tgttgaaagc
    11461 aacaatatcg ctcgtgaaag aaaacaagtt ttccataatt ttgaaaaatc agcttatgct
    11521 gacggcactt atttcgaaaa atacgtaact ggagaattcc agccaaaatc agaaaaagtc
    11581 aaagcattgt ttgaaggcat ttttatccca actgctgaag attggaatgc tttgaaacaa
    11641 gcagtaatga aagatggact gtatcatcaa aatcgcttgg cagtagcacc aaatggctca
    11701 atttcatata tcaacgatac gagtgcaagt attcatccta tcactcgcat gatcgaagaa
    11761 cgccaagaga agaaaatcgg taaaatttat tacccagcgc cttatctatc caatgagacg
    11821 attccgtatt acacttctgc ttatgatatg gacatgcgta aagtaatcga tgtttatgca
    11881 atggcacaac aacacgtcga tcaaggcatg agtttgactt tattcatgcg ttctgaaatc
    11941 ccagaaggca tttatgaatg gaagacaaca acgaagcaaa cgacacgtga tttgaatatt
    12001 ttaagacatt acgctttcca caaaggaatc aaagccattt attacgtacg tacctttact
    12061 gatgatgcag aagaaatcgg aagcaatcaa tgtgaaagct gcgtgatcta atatcataga
    12121 tagattcgct tattcatatt ttaattaaag gagcattctc atgtcagaaa catattatga
    12181 agcaataaac tggaatgaaa ttgaagatat cattgataaa tccacttggg agaagctgac
    12241 agagcaattt tggctagata cacgtatccc actttcaaat gacctagatg attggcgtac
    12301 attgtctcaa ttggaaaaag atacagtagg acatgtattt ggcggtttga cactgctaga
    12361 tactgtccaa tctgaaagtg gcatggatca gttgcgtaaa gatgtccgca ctccccatga
    12421 agaagccgtt ttgaacaata ttcaattcat ggaatccgtt catgccaaaa gttattcttc
    12481 tatttttagt acactgaata cgaaaaaaga aatcgaagaa atttttgact ggacaaatac
    12541 gaatccttat ctgcaaaaaa aagctgagcg tatcaatgaa atctacaaac acggtacacc
    12601 gctagaaaag aaaattgcca gcgtcttttt agaaacattc ttgttctatt caggttttta
    12661 tactcctctt tattatttag gaaacaacaa attggctaac gtagcagaga tcatcaaact
    12721 aatcatccga gatgaatctg tacatggaac ctacatcggt tacaaattcc aattaggatt
    12781 caacgagtta cctgaagcag aacaagaaac gttaaaagac tggatgtaca atcttcttta
    12841 tgagctttac gaaaacgaag aacgttatac agaagaatta tacgatccaa tcggctggac
    12901 agaagaagtc aagacattcc tacgctataa tgccaacaaa gcattgatga acctagggat
    12961 ggatccactc tttccagata ctgccaatga tgtcaaccca atcgtaatga acgggatttc
    13021 aacaggcaca agcaaccatg acttcttctc acaagtcgga aacggctatt tgttaggtca
    13081 tgtagaagcg atgaaagacg acgattattt gattggattg gaataaggat ttattttaat
    13141 tcaaaaattt ttcgaaaaat aaaaagagtt tagcgtacag atattttaaa tgtacactga
    13201 actctttttt atgcttattc acttgtttct tccagcactt ttggactgtc taacttttcc
    13261 gaaacatgtt ccattcttct caataaatag attttccgga tatttaatat tatcgtctta
    13321 atcactgcat agacaggtat gcaaatcagc atgcctaata ttcctgcgac acttcctgct
    13381 ccaatcaata ccaagatgat cgtcaaagga tggatattca ttgatttccc aaataacaac
    13441 ggcttgatca aatttccatc taattgttgg atcactaaaa tcgacaacgc catatacaaa
    13501 gcttgaaatg gagagataaa caaaccgaca atgattgctg gtaccgctcc aataaaaggg
    13561 ccgacatacg ggataatatt cgtgattccg caaatgaccg caagcaacaa gctataaggc
    13621 tgatcgaata ttctcattgc caaaaagctc attacaccaa taatcaaagc atctaagatc
    13681 gtactgctga tataggcaga taatgtttcg tttaattctt ttaccgtttg gcgtaactct
    13741 ttacgaatcg ctttcggaaa gaagtgagac aacgcatcaa taaatttgtg cccatccttg
    13801 aacataaata gcaaaataaa tggaacggta aataacaaaa taaaaaattg catcataaat
    13861 gaaatgattt ttgagacact tgcagataaa ctgactaata tgatattgaa caaattacta
    13921 agagataaat ttgctgatgc taattgttct tccaaattga aatttttaaa ctcttcccgc
    13981 tgcgccaatt catttaacca tttgcttgtt tcatctgcat acattggcaa gctctgggtc
    14041 aattgaattg tctgttccat taactgaggc actacattca tgacagttaa aacaactaaa
    14101 atgattacga ccaaaaacgt caataaaaaa ccgaacactc tcgggatccg atgcttttcg
    14161 agaaaaacaa cgattggatc gaacatataa tagataaaac cagcaacaag caatggtaaa
    14221 aaaactgccg aaagcatttt caatattgga ctaaaaatat tcggcatttg taaaataaag
    14281 tacacgccga tgatcgtgag tactagctcc actgtccaga aaaataattt tgattgttta
    14341 aaacgtgatt gcatacatct cctccaaggt cctttgatac ttctattcta tcatttaaca
    14401 aagagttacg cgctgagatt ttcatttgct taaaaaaaac aaaaaaaata aacatatttt
    14461 aatgagtaga cgagtactta aacaaaaaca cggtttggag tagtgacaaa aattatactg
    14521 cagtcccttt ttccaaataa acggtgttca aaagcttgca tcttcgtaat aagcccagcc
    14581 aaacgaaaaa tataaggaac tatttttatt tggctgggct tattacttga tgtaggacat
    14641 ctattttaca acctcagatc atttattgcg caatactgac taaatgcaac tttgtgatat
    14701 ttttgtgtac ggacaactcc tcgatcacat cggcataagt gagccctttt ggaagatcaa
    14761 tcgtatagat atttttgtac actcggtagt catcaacgaa ttttacgtca aaattcacat
    14821 cttcgattac gatatttttc tcttcaaaat aatcggtaag aaaatctttg gtttcttttc
    14881 tatggataaa ttggatttct aactttttag tcgtaggtac atggatcacg cgtttcacta
    14941 ctgtgagtgc aatagcaatc ccgataaaac tgatgatcgc aatggaatag taacccatgc
    15001 cgatggcgat ccctaatcca gcaattgccc ataaagaagc ggcagtcgta agtcccgtca
    15061 ccgattgttt cgtgacgatg atcgttcccg cgcctaagaa accgacacca ctcaccactt
    15121 gagcaatcag ccttgcttca tctgaacgaa tcgttccagc aagctgtgga tactctaaag
    15181 catctcttaa tgcaccacta gcgatctcaa cttggatcaa tgcaatgata catgctccca
    15241 tacaaactaa aatatgcgta cgcatacctg ctggacgatt tttatattgt cgttcaaaac
    15301 cgattgctcc gccaaatagc atagcgatcc ctaaacgtaa aataatctct ggaattgtca
    15361 ataccatatc attcatctaa acaccctcca gtccaatata tcatgtctga aaggagcaga
    15421 caaaaaaagt gcaattttta cctcttacga aaaaaggact gtgacaaaat ttttgtcata
    15481 gtccttttta tgaataaacg gtgttcaatc agctgaccct cggtattact caacaaacgg
    15541 caaaatatgg ggagctattt ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt
    15601 tcacaacctc tttagcctac tattgaaaat tacaacttac ttgctgcttc ttcatcgatg
    15661 atcacaacaa cattgtcgtg attttgcaat acacttgctg gcaaatgatt cgttacaggt
    15721 ccttcaatca tccctttgat tgcatcagct ttgctttcgc caaatgccat caagacgatt
    15781 tctttacttt tcataattga accgattccc attgaaacag cttttgttgg tacatcttct
    15841 gctttatcga agaatcgttt gtttgcattg attgttgatt ctgttaagtc aactactgaa
    15901 gtcgttccgt caaatgaagc accaggttca ttgaaaccga tatgtccatt ttgcccgatt
    15961 cctaagattt ggatatcgat tggatgtgca tcgatgattt gatcatactt tttgcatgct
    16021 gcttccagat cttctgcttt tccatctggt acaaacgttt ctttgaatgg ttttttatca
    16081 aataattgga catccatgaa atgacggtag ctttgttcat ctgaaccgcc tagtccaacg
    16141 tactcatcta agtttacaga agtcatttca ctgaaatcca aatcgcttga agtcatttct
    16201 ttatataaag taactggtgt gcttcctgtt gccaatccta atacttttgc acctttgttc
    16261 ataccttctt tgatgatttc aaatgctttt tttccgcctt cttcagcgtt ttttacacga
    16321 atgatttcca tagtgattgc tcctttatat tttggtatag cccaattata gcattctttt
    16381 tttcttttta caataattag tatagaccaa attgcatttt tttcattttc ttgacaataa
    16441 ataggaatcc gtgttaggtt tcataagatg gttcaatcaa acaatccatg ttgcatgaaa
    16501 accaagcaag tgattcactg tggacgcttt atgtctgcag tttttttatg aaaaaggagt
    16561 tattatgagt atactgacat taaacaacat tacccagcaa tttggcgaca aaatcctcta
    16621 tgaaggtgtc gatctgcaat taaatgctgg cgaacatctt gggctgattg gacaaaacgg
    16681 agccggtaaa agtacattga ttaaaatcat cactggtgag atcttaccag atgacggaca
    16741 gatccattgg caaaaaaata ttcacaaagg ttacctcgat caatatgtgg aagtcgatga
    16801 aacattaaca atcgaagaat ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga
    16861 aaaaattgct tttctttacc aaacatatag cgagaccttc gatgattcct tgctggaaaa
    16921 agctgggaaa ctccaaacgg aattagacca aggagtgttt tatcaaatcg acaccttagt
    16981 tgctgagatg agcagcgggt tggggatcga tgtcttaggt ttagattcac tgttgaaaaa
    17041 tctaagtggt ggacaacgtt caaaggtgat tttagcaaaa ctacttttag aaaacccaga
    17101 tgttctgatc ttagatgaac caaccaatca cttagatgat cagcatatcc aatggctgac
    17161 gcagtttctt caggattttg acggtgcgta tatcgtgatt tcccatgacc aagaatttct
    17221 tgatcagatc accacccata tcgcagacat cgagtttgga aagattacta agtatactgg
    17281 ccatctaaaa caagcattga aacaaaagga acaaaatcgc gaaagctatt tgcgccaata
    17341 tcatgctcaa caaaaacaaa tcgaaaagac cgaagcttat attcgaaaat acaaagccgg
    17401 ttcccgatca acgatggcaa aaagccgaca aaaacaatta gataaaatcg aacggctgac
    17461 gcctccagct tcagcaagta aagctgtctt tgattttcca ttctcgccta ttgtcacgac
    17521 attagcagtg gaaacaactg atttagtcat cggatatgat cgaccgctac ttgcacccat
    17581 caatttgacc atccgctttg gtgaaaatat tgccattcga ggcttcaacg ggattggaaa
    17641 atctacttta ttaaaaacac tgatcggtga gattgaaaaa ataagtggcg atttccactt
    17701 cccagataac acaaaaatca attacttttc acaagatctg tactgggaaa atccattaga
    17761 aacaccacta caatatttaa gcagtcaatt tccaaaagcg actatcaaag aattacgtcg
    17821 ccaactagct aaagcagggt tagttaatca attagcaagt gaacctctca ctacattaag
    17881 tggtggggaa caaactaagg tgaaacttgc acagttgaca atgaaccaag gtaatttact
    17941 gattctcgat gaaccgacga accatatcga tcaagaaacg aaagaaagct tacaagacgg
    18001 gatacagaaa tttccaggaa cagtaatcat cgtctctcat gaacaagaat tttatcaaga
    18061 tcttgtgcaa cgagtcattg agattgaacc aaaataaacg tttgattgaa tagttgaaaa
    18121 aaaggagctg tgacaaaagt tttgtcacaa ctccttcttc tgaataaatg gtggccaatt
    18181 agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggagctatt tttatttggc
    18241 tattcttatt acttgatgca gaacagcttt tcacaacctc ttttcgtcta ttttttattt
    18301 tcgagatttc tctttccttt aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa
    18361 acaagcccgc taaccgttac acctaaccag ccatactgct gccacattgt cgtccctagc
    18421 aatgaaccag ccgcacctcc aataaagtac ataaacataa atacagtatt atttcggttg
    18481 cttgctgctt cacctagccc ttgtactcgt gtttgattgg cgacttgacc aaattgattc
    18541 ccgacgtcta ataaaataat agctaaaagc aaaatggcca aatggcttcc gccaaccaat
    18601 aaagcaataa agcttattgt ttgcataaca agtcccatat acacgatttt tctttcagaa
    18661 taagaatctg ccagtcgtcc cacgataggt gcagcaactg ctccagacaa accaaaaatc
    18721 gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg gactgctgat ataaaaaatc
    18781 aacgtagacc aaaagatcga aaatgttcca aacatgaaaa atccactgac tgccgcttct
    18841 cttaatagac gttgagaagc aactaaatgc ggcaaacttt taagtgaatc aaggtatgaa
    18901 acagctgtac tactttgctg gacagaacgc ggcattttcc agtaaagcag aagtgtaaga
    18961 attccgactg cacataaagc aaataaatag atcatccgcc aagaaaaaag actggcaaca
    19021 ataccagata ccgtccgaga aagcaaaatc cccacgagta aaccgctaag caatgtaccc
    19081 atgaccttcc ctcttgcttt tggtcccgct agtaccgcac cgtaaggaat gatgatttgc
    19141 ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg caaataacgt aaacgaaggc
    19201 gcaaaaaaag cggccaacag agagacggca gataacgctg ccattcgaat gatcaatttt
    19261 ggacgatcca ccacgtcccc taaaggaact aaaaacagta accctaacgc atagcctaat
    19321 tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa cagataaact agctgctact
    19381 tttgttccaa tcggctggat gtaatacata ttggcaacaa caacgccaca agtgattgat
    19441 aatagtaaaa taagcgaagt tgttaaaggt tttttttcat tactcttcat tgatttcccc
    19501 catttcctat cttagtttac aacttatgga aaagattgga aatagaaaaa tacaactatt
    19561 cgccacactt attatttgtg cttttgtttt ccttgttttt tcttgcgatc tttataaatg
    19621 aagacaacta accctactag cacagtaaca cttgcaacca acgtgactgc agttttgtta
    19681 tatccgtgaa acagagcttc tccaccacac gcatacaaca cagacgaagg gatgactcca
    19741 atcacgatcg acaatatgat ttgctgtaga ggcaatttca tcgcatctgc agcaaaattg
    19801 accatagaag atggaataac cggtatcata tagcctaaca ttacacctat ttttggatgt
    19861 ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg ttttcttatc taaaaatttt
    19921 aagcgatgca taaggaaaat tgcagtgatg ttaccaagag cattacccaa aatgttgatg
    19981 atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga ctccaactac tgaagtagga
    20041 atacctggaa cagcacacat caccgctgtc aatacgatca agagtatcga tgcaaaagtc
    20101 ccatgcgaac gaatatgtgc gagtagctct gccttactcg ctctcggatt taaaaataat
    20161 tggatgtctg tctgatattc taaatacaac cgatagccca ctaccagtat cagaatgatt
    20221 cccaacgaga tcaatatcaa tcggattatt ttctttttca tatttcctcc tgaaaaaaat
    20281 ctatattcat taaataatag tttaacataa aaacaaaaaa gcaccgaaat ccaacatagg
    20341 atctcagtgc ttttaaatag taaaaattat ttaacagtta cagatgcgcc aacttcttca
    20401 agtttagctt tcaattcttc agcttcttct ttagatacgc cttctttaac tggtgcagga
    20461 gcgccatcaa ctacagcttt agcttctttc aagcctaaac cagttgcttc acgaactgct
    20521 ttgataactt taactttttg gtctccagct gcagttaatt ctacagtgaa ttctgtttgt
    20581 tcttcagcag cagcagcgcc accagcagca gctacagcta caggagcagc agcagatacg
    20641 ccaaattctt cttcaatagc tttaactagg tcgttaagtt ctaggatagt tgcttctttt
    20701 aactcagcaa cgatgttttc aatgttcaat gccattataa tttcctccgt ttaattgttc
    20761 ttttttattt aatgctttat gcggcaatta agctgcatct ccatcttctt tgtctgccac
    20821 tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt agtacagata gcaacataga
    20881 tagaagtcct tcgcgatttg gcaattttgc caatgctgtg atttcttcca atgaagagac
    20941 tttaccttcg atgataccgc ctttgatttc taatgctttt gcttctttag caaattcgtc
    21001 catgattttc gctggtgcga ctacatcttc gttactgaat gcaacagctg taggtccagt
    21061 gaatacttcg tccatgcctt caaggccagc ttttttagct gcacgtgaaa gaatagaatt
    21121 tttgataact ttcatttcta cgttagcatc acgaagttgt ttacgtaaac gtgttacttc
    21181 atcaactgtt aatccgcggt agtcaacgac tactactgat gcagctgctg taaatttttc
    21241 gcttacttca tcaacgattg ctgctttttt tgcgataatt gcttcactca tttttgagtt
    21301 tcacctcctg aattttgatg gaagagtttt ttgcactgaa aaggcaagtc acttttatag
    21361 ttactttgct caaccagtat tattcatcac ctattcactt cgttcatagt gattctactc
    21421 gctttgctcg aaaggaaact cctttcgctt tgctcaacca gtattgttca tcatctcttc
    21481 acttcgttca tagtgattca caataaaaaa ctctatgcca ccgcagacat agagggacta
    21541 ttgaatatat tcaataaacg tcctcggtag gaaattaagg cttgatgcca cctactgtct
    21601 tcggtacaag ttaattatta acctcaacta gcttagcatg ctaaactagt tgagtcaaga
    21661 attatttttc agttggatca atcaacattc taatatttaa ttagaaagaa gcttggtcaa
    21721 catggatacc aggtccaaat gttgttgtga tcgttacatt tttcatgtat tgacctttag
    21781 ctgctgatgg tttagctttt agcaatacat catggattgc tgcaaagttt tcaactaatt
    21841 tttcatcgtc aaatgaaact tttccgattg gtacatggat atttccagct ttgtccacac
    21901 ggtaagttac tttaccagct ttcacttcgt ttactgcttt tgttacgtcc attgtaactg
    21961 taccagtttt agggtttggc attaagcctt ttggtcctaa tactcgtcct agacgaccta
    22021 cagtagccat catatcaggt gttgcaacaa ctacatcaaa gtcaaaccat ccgttttgga
    22081 ttttttgaac catgtcgtcg tcacctacga aatctgcacc agctgcttca gcttcttttg
    22141 ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt accagttccg tttggtaata
    22201 caacagcacc acggatttgt tggtctgctt ttttagggtc tacgttcaaa cggtatgcaa
    22261 cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt tgctaaagct actgcttcgg
    22321 caacaggata aactttactt gaatcaactt tttttaatgc atcttgcatt tttttgctct
    22381 ttttagccat tttggttcct ccttgattgt ggttataacg gttgtacctc ccacgtgttc
    22441 tatatctttc aatatagagc caactgagaa gctgctgtct tagggtgtat agattattct
    22501 acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc gcattgctgc ttcaacgtta
    22561 gctgcgttta ggtcttccat ttttaattca gcgatttctc ttacttgatc gctagaaact
    22621 ttcgcaactt tatttttgtt tggttcgcct gaaccttttt cgattttagc tgcttttttc
    22681 aatagaacag cagctggcgg tgtttttgta acgaatgtga atgaacggtc ttcgtataca
    22741 gaaataacaa ccggaatgat caaacctgct tgatcagctg tacgagcgtt gaattctttt
    22801 gtgaatccca tgatattgat acccgcttga cctagtgcag gacctactgg tggagctgga
    22861 gttgctttac ctgcaggaat ttgcaattta acgatttttt ctactttctt tgccacgaga
    22921 catacctcct taagtccgtg atgtggttaa ttggagatgc atatttctcc tcccacgttt
    22981 tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac aatgcacact ggtacagtat
    23041 agcaaattta tttgtttttt caagattttt ttctgctatt tttacatttt gcttgaataa
    23101 acgctaaatt tattctaaaa aaacaacaat ttttcgttct catttcttct tttatgttac
    23161 acttgcgcta gcatggttca acagatgaaa gcaaacaata acacggtttt ttagaccctt
    23221 gtacaaagat tcctacataa caaaatgagg tggagaaaat gaaagattta aggtatagaa
    23281 gtgtctttga tattattgga cctgtgatga ttggtccaag tagctctcac actgccggag
    23341 ctgctagaat cgggaaaatc atacgtagta tatttggcga acagcctgat tctgtagata
    23401 tttatctata tgaatcattt gcaaaaacct atcgggggca cggaacagat attgcattag
    23461 ttggcggcct gttagggatg gaacccgatg atgaacgttt agctgattct ttgaaattgg
    23521 catatgaagc cggtatggaa gtcttgtttg ttcctaagag tgaaaaagcc gaacatccaa
    23581 actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct ttcagtcact ggcatttcga
    23641 tcggcggtgg caatatccag atttctgaat tgaatggatt caaaatttct ttaagcatgg
    23701 gtactcctac cttgatcgtt gtccaccaag atgttccagg tatgattgct aaagtgacta
    23761 gtatactttc tgagaccaat acgaatatcg gcactatgac agtgacacgg gaatcaaaag
    23821 gtgaaaatgc cattatgata attgaagtag atgatcctca agtagaagaa acggtaaaaa
    23881 gactcaaaca gctgccaaat atcgacagcg taaattattt cgattaagga gctaaaaaaa
    23941 tgtttttatc gattgaagaa ttagtagatc aagcaaaagc ttatccaagt gtagcagaat
    24001 tgatgattgc agttgaaatg gaaatgagtg gccgttcccg tagtcacatc atcgagatca
    24061 tggaaaaaaa cctctcagta atggaacaat caatcaacga gggaaaagaa ggtgttacct
    24121 ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga tgattattta aatgaggcag
    24181 actttttaag tggagaaaca atcttaaaag ctgtacgcaa tgctgtagct gttaatgaag
    24241 tcaatgcaaa aatggggttg atttgtgcta caccgactgc cggaagcgcg ggtgttgttc
    24301 ctggtgtgct gatggctgtc cgagatcggc ttgatttgac acatgaacag caattggaat
    24361 tcttatttac cgctggcgct ttcgggttag tcatcgcaaa caacgcttct atcagtggtg
    24421 cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc aatggcagca gcagctttgg
    24481 tttgtgcaaa aaatgggact cctcatcaag cagcacaagc agtagccatt acattgaaaa
    24541 atatgatggg actgatttgc gatcccgttg caggcttagt tgaagtcccg tgtgtcaaac
    24601 gtaatgcttt aggctcttct caagctttta tctctgctga tatggcatta gcgggtatcg
    24661 aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta ccaagtcgga cgtcaaatgc
    24721 cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt tacgccaact gctaaacgac
    24781 ttgcggaaga gattttaaat aagagggcgt gataaagctc cttaatttcg agtattaaga
    24841 gattagttat cttcataaca aaaaacgaag agaggactaa ttgcccttct cttcgttttt
    24901 tattaatcca tcaatttcgt attttttact tcatcgatcg tttttgttcc tgcaagttgc
    24961 atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag atgttactcc ttctgcccca
    25021 cctagattca acccataaat cactggtcgg ccaatcgcaa ctaagtcagc accacttgcc
    25081 agagctttga agacatgctc tcctcgacgg acaccagagt cgaaaatcac tggtacacgt
    25141 ttgttgacta cttcggcaat ttttggcaat acttcaaagg acgcaggacc tccatctagt
    25201 tgacgtccac catgattgga tacccagata ccatctgccc ctgcagagat agcgattact
    25261 gcatcttctg gcgattgtat tccttttacg ataactggaa gatgggtgat ttctttgatt
    25321 gttttgatgt catctggtgt caagccttgt tttgcagctg cataaatttc tgcgattcct
    25381 tttccttcgc cattaccact tgcactttgt tcgctatacg cagctaagtt aggcattggt
    25441 aatggaaatt ggaattgatt gatgacgtct tcttcacggt aaccaccgag cgttgaatct
    25501 gccgttaaaa tgatagcttt agcccctgct tctactgctt tgttcaaaat gaactcattg
    25561 aatctgtcgt ctttactcat atataattgg aaaaactgcg gtgcgcctgg cgctgcatcg
    25621 gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg aaaagattga tccagcagca
    25681 gctactcctt tagctgtatc agcttcccct ttttcatgtg ccaatccttg ggccgctgat
    25741 ggcgcttgga taatcggtgt atctaattca attccgaaaa cagatgtatg caaatcggct
    25801 gagtcgattc cgcgtaatat ccgtggcatg attttctttg tattaaaaga agtcgtattt
    25861 tctttcatcg tccactcatc ttcagaacca ccgcgaatat agccaaaagc tcctgcttcc
    25921 atgcgctctt ttacacgacc ttctagttag gcaatgttta caatatcgat tggatgttct
    25981 tcattacttg tatgataagt catagtagat ccttctttca tcttcttgat tacatataaa
    26041 tcatactaaa cttacaaaaa gtaagcaaac atttactctc agatactttt atcgctattc
    26101 tttccaaata aatcgactta caagctgatc tacttcttgg ttttcatgaa gcatactatg
    26161 agaggcattt tcacctgtca ctactttttc ctctatttca tttccgcgtt gcttcaacaa
    26221 cgcatagacc gctaatgcac tgtttaacgg tactgttcca tcactcaagt ctgtttcatc
    26281 tagttgacca gcaatcaaga aaaatcgagt ggttatagga atattaccga tcatttgctg
    26341 gtaatcttga tagcggttag aaacgacagc aggccccttt gctaactcat ctgtcagaga
    26401 ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca attgctgtta atgtctgaac
    26461 ttgtggcaat gacgtatcct gtccatacgt tcctaaatag cgtagtgcac taactcctcc
    26521 catggaatgc cccactatgt tgactttatc tatgccgtag ttttctttca gatacaaaag
    26581 acaagttttg atccattctg cttgattcca ctcgttattt ttattatctt caaacaatac
    26641 ttggaccatc ggattgtctg cttttccaga caaatctcct gttgcttgta tttgtccatc
    26701 tgttcctact gttaaaacca attcttttac tgcttgctga tttttttcca aacgccgcaa
    26761 catcccttta aaggaatttt ctgtcccgct gtagccgtgg aaaaaaagtg ttggaatagt
    26821 ggttttttct atcactttac tggattctgc cgttttttct ttatcagaaa cttcagtagt
    26881 atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa ctgataagga ctaaagctgc
    26941 tgcagttgca cgtttgcata aatttttgat tggttctgtc atacacctct tcctttccat
    27001 atagaaaaaa gaaagacttg aaaggccaat catatgatcg acatttcaag tctctttatt
    27061 aatcgatttt atctacttga tcgaaatcaa gttctgtact ggtttcacgt ccaaacatat
    27121 cgatgttgac tttcaatttt tctttttctc cgtcaacttc tgttacttgt ccttctaatc
    27181 cagcaaatgc gccttcgatg attttcacga cttcgccaac ttctacttct agatcatttt
    27241 gtcgcaggct cataccgata gaacgcaaga tatgattgac ttcttctgga agtaatggtg
    27301 ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt cacaccaggt gtgttgcgga
    27361 caacatacca tgaagcgtct gtcatgatca tttcaacaag tacgtatcct gggaatgttt
    27421 tatgaacgat ttctttttct ttcccatttt tgacttcagt ttctttttct tcaggaacaa
    27481 cgacacggaa aataaagtcg gccattttca tactttgcgc acgagattcg atatttgctt
    27541 ttactttgtt ttcatatcct gaatatgtat gcaagacata ccagtttttt tcaaaagatt
    27601 ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc tcagtacacc cgaagttttt
    27661 ctttcgcttc attatagcac taaaagtacg ttgtggctag cgagttattt taaaatccag
    27721 ccaaataagc tttggatggc tgtatccatt acgtaaaaca tcaatccgaa aataatagaa
    27781 gtttcgatga ctactaacgt atctttacgg agttgttttc tgcttggcca agttacttgt
    27841 ttgatttcat cttttacgct ttttagaaat ttcatgagac aacctccctt gctactgttt
    27901 atttcgtttc tttatgtaat gtatattgat tacaatattt acaaaactta ttgatttcca
    27961 aacgttcgcc gctttttcct tcactgactg attttgtgta gttgcgagac ccacaaacgg
    28021 aacaagcgag tgctgctttt ttctttgcca tttacttctg cccccttgct gagaatcaga
    28081 cttatacttc ataacagtat cacgtaactt tttacttgtc aatttgtgaa ataaactatc
    28141 tattgttttg gataaaatta tgatatgatt aatttgaaat taagctaaaa tgagacaatt
    28201 ggaggtatag cgatgctatt aaaaacactt gtatataaga aaaaagatct gacaaccgtt
    28261 acagagacta gtacattaga agaagcatta aaaattttag aaacttccgg ataccgttgt
    28321 gtgccgatct tagattcttc aggtaatatt ttccgtggaa acatttacaa aatgcatatc
    28381 tatcgtcata aagcaaatgg cggtgatatg acgcttcctg tcactcatct tttgaaaaac
    28441 gctacaaagt tcatttacct ggatacttct ttcttcaaag tattctttac gatcaaagag
    28501 cttccttata ttgctgtcct tgacagtgaa aatcattttt atggtatttt gacacatagt
    28561 acgttgctta atatgctctc tcaatcttgg agcgtagaac aaggaagcta tgtactgaca
    28621 attgcttcca caggaaagca aggagatttg gctgctattg caaaaatcat ttcaaaacac
    28681 agcagcattg ccagctgtat tactcttgat atcgaaaaag acgaatacat tcgacgtaca
    28741 ttgatcacgc taccggctaa cactgaagcg aaagtatgtg aaacgatcgt caaccactta
    28801 gagaaaaaga attataaagt ggtagaaatt gaaaatttgc aaaaccaaga agcataatca
    28861 aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt cgctttttat aattagacac
    28921 gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa tggtgctgaa acagccatga
    28981 cgataaactc acttaatgca agcgtgccat aggttgtcca aaatgcttgg ccacctgttg
    29041 gaactaacat ataagcaatc agtcccatgc tgatggtaaa gaagatcgtg ttcaatacta
    29101 aacgcatctt tacatttttc actttgtttt gcaataaagc tgtcaggcct aaagaaagca
    29161 atgtttggcc gccaccaaaa accacatcca tgataccaaa accaaagaag gcattataga
    29221 cgatgacccc gcctaaaact ccccaaagca atttacggtt aaagacaact aaatgattca
    29281 aactttctga aatacgaaac tggatcggcc ctgaggatac aggcgcaact aaaatagtta
    29341 acgctagata aagagccatg atgatgccat ttacaacgat tacggataac cgttgtctgc
    29401 ttttcgtttt ttgcattttc tctcctcttt tctcctagtt tttttaacgc gggatggttg
    29461 atgaaccgcg gttatgtgaa acaaacaata attataacag acttttcatt tatttcaatc
    29521 cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg taaacgtttg ctaaaaatcg
    29581 tgggatatgg tataaagtaa gtgtaagggg tcataagaat tcccagtaaa gcagacgaag
    29641 taggatctgc aagaacagaa ttgttgtgaa acttaaataa ataaaaaagg atgtgggaat
    29701 ttgagcaacc tcttactaga gaacggtcta gcaagcagct gaagatctaa gtggacatgc
    29761 cgaaccttgg aacttctcct tacgtaagta atggatcatg cgcttgctag gcttttttgt
    29821 gtttttcggt cttcggacct ttttcatttt cctagacttc tgctataata accaagaatt
    29881 aattttgtaa cagatttttt tgaaaaaaca agtttctttc attcaaaaaa tagaaaaagc
    29941 ttgctaatta ttggttaacc agcaaacagg agtattaaaa tatcttgttt tattgaatag
    30001 caggaggaaa tcatgaaatt ccattatcat atattgatca atccatcggc aggcagcgga
    30061 aatggctata aagtagcaga aagaatttta ccggtattaa aaaataagca tattgactat
    30121 acacttcatt atagtgaata caaaggacac gatgctgaga ttgctgaaac tttggcaaaa
    30181 gaaacattga ttccttggat agaagaagaa cagaaaacaa tcgatacttt cccccttttg
    30241 atcatcgtag gaggtgacgg caccttacat caggtacttg atactttcta tcagatggaa
    30301 gtagaattcc cagttgctta tattccagca ggttctggag atgattttgc tcgaggggca
    30361 ggccttccaa aaaatcccaa aaaaggactt cagctgattt tagcagctca atcaccagaa
    30421 aaagtccaca taatggctta tgaagaaaaa ataagtgaaa aaaaaggaat agctgttaat
    30481 aatttcggta taggattaga tgcagctatc gtccatgcga caaatcattc ttccacaaaa
    30541 aagcgattga acaaatacaa cttaggatca ttttcgtatt tattctctat tctccgtgct
    30601 ttattcactc aaaaaggatt tccaattctg gtagatgcag gaggaaaaca attaagtttt
    30661 tcaaacgcat ttttatgtac agcaacgaag catccttttt ttggcggagg aatcgcgatt
    30721 gttccaactg ctgatgcgtc taaaccagtc attgattttg tcgtggtcga acgaatcaat
    30781 ttctttaaaa tcttgtggct cattctttta cttatacaga aaaaacaatt gaaatcgaaa
    30841 tatttccatc actacacaac aaacagatta cgtatcgttt caacaacacc gcaacatggt
    30901 caagaagatg gagaagaaat ggaaaaacga ccttttgata tcacaataac cacaactaaa
    30961 cagttgtttt ggtgttaata aaagaggttt tgtcagaagc tacatctgga acaccgttta
    31021 ttcggaaaaa aggactgtga cacgactttt gtcacagtcc tttttttact atgcagttaa
    31081 ttctttgccc aattgggttg ccatcgatag tttctttctt tacccaaaat aaattggatc
    31141 attgaaaaca ccgcagcaaa agcaactaag acacaaccat tgaaccaaaa agattctgca
    31201 aatgtttgat tgtaactgag tgtttcactg atcaagttat tattttgaac aaaccaatat
    31261 gttggtgtga atgccgctat tttgctaacc atttctggca agtattctgc tggtacaaac
    31321 accccaccga taaagcaact gcctgtgata aaaatatctt tgatcccacc gctggtttct
    31381 gagtttttta ccaatgaagt gatacaagcc gaaaagctaa tgatcggcaa taaaaacaag
    31441 aaagtattta aaatatgata ccataccgtt atatcccacg aggtgtggat cactaacaga
    31501 accattagaa gtaagaagcc actgaacaac agcagcgagt aactaatcaa actaaaggtt
    31561 actttgcgat tcaagtttcg tttggtaatg ggcgcactct ggtttcgttt actgatctct
    31621 ttgcgattaa atgctagatt gatgacgctg aaaccgctaa aaatggatag aaacaaccca
    31681 taagacaaga aattaaaggt tgtgcccgtc aacagctttt tcatccgctg actatatccc
    31741 gcacttagct caacttctcc agtaaccgag agtgccattt gtgtctgttc catagctgca
    31801 tcgattgttt tatcttcttc ttgaaacaga atcagtgttt gcaaaaaggt gttcacttgc
    31861 tgtgtaacaa ctgttttaga aaaggcatct gggcgactct gtatcttgat cgtcggcatc
    31921 tttcctactt cgacttgttc ggtaaagtct gcaggcagat gtaagataat gtttacacga
    31981 ttaaaataaa gagcatcatc gatttctttt tgtgaggttc catccaatgt aacgactgtt
    32041 tccttgtcat tcaaatattt acgaaggcca tcgactacag gcgactcttc ttcactaatc
    32101 aaggcgattt tcgcatcttg cagagaggta ttttcaacat caatattttg cgcaaaaaag
    32161 aatgtggcaa caacagtgat aattacacct atcagtaaat tcgatttatt tgctttaagc
    32221 aatgtccaaa tcgttttata aagcgtcata acgcacccct ctttccatcc aagcatttcc
    32281 taacgcaaaa aggaaaataa atccgatcaa ccatagaaga ttggtataaa aactagccaa
    32341 atcagtgtag aaataaagtt tatacaaact ttcgctgatc aagttgacga gatttaattg
    32401 accaacaatc ggaatatgaa gatcaatcca atacttcact tgttccgacc ccatcatacc
    32461 agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg ctatcctttt gcaccagatt
    32521 catttttggt atcaaattac caaccaatgt acctaacaaa atcgcacaca acgaccccag
    32581 agcggtaaca atcatcaacc attgccaacg ttcaccaaag tctacttgat agataaaacg
    32641 aaagacagct aacataatat aaatctctgt caaaacaatc gtaaagcttg ctgccaaatt
    32701 agcgattgaa actaacgatt gctttctagg aatcaaggac aagcgaatac catttgacga
    32761 ttgatttgct tgctgatcct gtacattgcg gaccccccac atcgtcccgt agagaatcgc
    32821 cattccaatc agagtgaaga aataaaaact tttgaaactc atattacgac ctgcatgaac
    32881 ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg ggcgcgagat caacgttctc
    32941 cacttctgcc actttactcg aaattatacc aatacgctgt aaaaattgat ccatgacgtt
    33001 tttcatcaac gtctgactca ttcctgcttg acccacatgg agcgaaacag atgaaccatc
    33061 aatgagataa aaaccagcca aatcatctgc atttagcttt tcattcgctt ctacttccga
    33121 tagttcctgt acttcaaata atggtttatc attgacttcg acttgttcta agactgtggt
    33181 aaaattttcc gaaagcgctg tttgatccgc tttgattaca attccagtat taatcgtttc
    33241 gagattgcca ccttgatcaa tctcgccaaa ggccgccata aacatcaatc ctaaaagaat
    33301 tggaaaggcc aatgtccaaa acaacatgga tttatttcgc agcagtattt ttgtgcgata
    33361 tttataaaaa tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc ttgccagtaa
    33421 tctctaaaaa gacatcattc aaggacgctt ctttggttgc tatttttccg taactgatct
    33481 tttcttcttg taagattgta agaatcggca acaatactgt atttagttca gtagttgtta
    33541 cttggagctg acctttttta taagcgattc catcagaagc tattgttcgc tgacaacacg
    33601 ttttctggga gtaatgggat gtcgatcgtc aaacgatccg ccgtccgtac catatctttt
    33661 agagattctt tggttccttt agcaatcacc tgtccttgat ccatgatcac gatctggtcg
    33721 caaagaattt ctacttcttc catgtaatgt gttgtataga caatggttgc tccttgtcga
    33781 ttcagctcct tgatactttc taaaatttta ttccgacttt gggggtctac agctactgtc
    33841 ggctcatcta aaaaaattaa ccgcggcttg tgggcaatcc cacatgcgat gttcaaccga
    33901 cgtttcaagc caccactaag ttctcctgga cgaaacgaaa catatttttc taatcccact
    33961 aattgaatca cctcttcaat caaggctttc cttttctctt tttcggaaac atataaaccg
    34021 caaaaataat cgatattttc ttgcacactt agttcgtcaa agaccgcaac gtcttgagga
    34081 acgaccccaa tcattgcctt gacagcataa ttttcaggag tcattgttgt cccgaacaat
    34141 ttaatggttc ctttgtcata atttaacaaa gaaagcatac agttgatggc agtcgatttc
    34201 ccactgccgt ttggtcctaa cagacctaag acttctcctt cttgtacttg cagatcaaaa
    34261 tgattcaatg ctacaaaatc tccatagcgt ttgactaatt tatcaatttc gataatcatc
    34321 atgaaaaact ccttccaaat ttcttgttga tctgactata ataaagtgca agaaaagaaa
    34381 ctagtgacga ttggctaaac cacacatgac atttgtcaca atgaaggagt ctgctatgca
    34441 taaatctcgg ctactcaacg aaactagctt tctactggtt atctcattgc ttttgctggt
    34501 aagtcaagga ttcgttgcta tgcaagttat ctatttattg atcgccgttt tattcactgg
    34561 tgtcagttat ttactgccta caaagccaaa ttggattttc caagttattc tatttctttt
    34621 atgtgccttt ttacctgatc ttgtcctgtt tctgccagcg acgatgcgag tgatcgcccc
    34681 tgaagaaagc caccttgatt acttgttttt gttaggtttt ttgttgattt cgacggttgg
    34741 taatcatccc cccatcaagg tcgcctttgt cgtaattttg ctgttttttt ccttttactt
    34801 acgaaaaaaa gatcaacgaa cgaccgatct tgagaaaaag tacattgtct ccaaagatac
    34861 tcattgggaa aaacaacaag agttatcgga acaaaataaa gcattggccc tgtcacaaga
    34921 aacacaatta gccttgcaaa ttgtggaaga acggaaccga attgcccgag acatccatga
    34981 caatgtcggt catctgctgt caagtgcgat tttacaaatt ggtgcgcttg aaatgatcaa
    35041 tcaagaacca aaactaaaag aaccattaga aaatttaagt gccaccgttc acactgggat
    35101 ggatcggatt cgtcaaagtg tccatgacct ccatgagaca tcgatctctt ttcaacactc
    35161 cttagagttt ctgatcgctg attttccttc ttctgttacg attgaaggaa acttgtttga
    35221 aggattgaat gaaacgcagc aaaactgctt cttaatggtc atcaaagaag ccttaaccaa
    35281 cagtatcaaa catagtcatg ccaaaaccgt tgttttacat tttcgaacgt tgccggcctt
    35341 ttatcgcttg caaatcacga atgatggcac cagcccacaa aaaaattctg ctcccggtat
    35401 tggtttgaaa acgatgcgtc aacgtgttca agaacaaaag ggacaagtcc atattttcca
    35461 agaaaaccag caatttcagt taacgatcat tttaccaagg gagggacacc aatgataaca
    35521 gtagtcatta ttgagatgat ccatttgtga cgaaatcgtt acaaaccatt ttagaaagca
    35581 cgaaagagat ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcta gtcttatacg
    35641 aaacccatcg tcccgatgtt ttgctgacgg acatccgcat gcctaaacaa acgggcatcg
    35701 atgctgccaa agaaattttg gctaaatttc ccaaagccat ccttttgctt ttgacgacat
    35761 tcaaagatga agaatacatc cgcgaagcat tttccatcgg tgtcaaaggg tatttaatca
    35821 aacagaactt gcaagcgatt attccttctg tcaaatccgc ttataatgga caggccgtat
    35881 ttggtaatga aatcatcgag actttcactc aaatgatgaa gaataaaccg acgattgata
    35941 gtcatgctta tgcttctttt agtgaacgtg agcttgcaat catcaaagaa gtagctgccg
    36001 ggaaaaacaa taaagaaatt gcagatgcct tgtacttgag tgacggcact gttcgcaatt
    36061 acatcagcca attactagaa aagctagatt tacgggatcg cacgcaattg gctatctatt
    36121 tttatcagca tttgcaataa gtcttatcta acacaaaaaa acctgaatga tgctccttcg
    36181 catcattcag gtttttttca catcttctat tcaattgtcc cgcaaaccat ttcctcacga
    36241 ttgaaagaat caaggattcg cagggctgcc aggattcgtt ggattgttcg gcgtgttagg
    36301 cggaaaaata ggcgccttgt ctaacgtttt ttctagtttt gattcttcac gatcgtctcg
    36361 ttctacatca ggactttcat tcaatttgaa ttctttttcc atctcatcta actttttaaa
    36421 gtcattgcca gattcttttg ctaatttcgc taatttatct tcggtttctt tagccataaa
    36481 gtaacgcccc ccttttgatt tcagcttagc atatcacact gaaaaaacaa aagaaaacgc
    36541 taaccaacga tcatttttag ctatttctca tctttaaaat caagcatttt ttgccggtat
    36601 aaaatcgtag ggagaactgt tttccatacc aatcatcgga tctactaact ggttatcgat
    36661 cacttctgct gtaagaacga aatctttaga aacagcaggt tctacctctt ctttcttttc
    36721 ttctccaaca aatgaagaaa acagatctgg ttccactgtt tccattactt ggtttttcgt
    36781 ttcattcgtc ccactgtcat cttctacagg tagcttttct tttacaaccc gtggctcttt
    36841 tgtatagttc ccatcttcaa atggatcatt tccgaccaaa gaatcttcat aagctgctag
    36901 tttggtccgg agagtgatat tcattttatc attatccatg atccttgatt tcaacgtagt
    36961 catccggctt gctgattcat tcgctacaca agtgacatag gcttgctctt cccctaacaa
    37021 aatcaacagt tccttactgc gagtcactgc cgtgtagagc aaattacgct gaagcatccg
    37081 ctgatattga tggaccatgg gcaaaataac catcttgaat tcactgcctt gtgatttatg
    37141 gattgagcaa caataagaaa gagtgatttt attccattcg tttcttttat aagtgacctc
    37201 atttgcatca aactggataa ctaattcatc cactttatct tcagaatctt ttgccaagat
    37261 gatacccgtg atttctccca tatctccatt gaatacattc aattcgggag tattgactaa
    37321 ttggaggact ttgtctccta tccgataaac ggtgtcgttc caatgtactt ccttttttcc
    37381 aggtttccct ggattgaata tctcctgcat cattttattt aacgcatcga tccctgcagg
    37441 tccccgatac attggtgcta atacttggat atcttgtgca cggaatccct tatttttcgc
    37501 tttttccaca attttagaga tcaagggttc aatttgataa gcatcacttc ggaaatagga
    37561 acgatctttt tgattctttg tgaaatcttt tggcagacgt ccttctttga tctcatgggc
    37621 aagaggaata atactagaac catcaccttg ccgataaatc tgattgagtt ccattttggg
    37681 aatttgttcg atttccaaaa gatcatggag tacttgacca ggaccgacag agggaagctg
    37741 atctttgtct cctactaaga taacttgcat attcgtcgga atcgctttga ataatgtgtt
    37801 agccagccat gtatcgacca tagacatctc atcaacaatc aaaagtccgc cttctaattc
    37861 tttcgtactg actgaaggtg ttttttcccg tccattcagc cccagaagac gatggatcgt
    37921 actggcaggc agacctgtcg tttcgttcat tcgtttagct gctcggccag ttggtgctgc
    37981 aagtagaata ggaaaaattt cttgggtata ttttgaagga tccaattcca gtccgttcaa
    38041 ctctgcaaaa agattgacga ttccgttgat cacagtagtt ttccccgtac ctggtcctcc
    38101 tgttaaaata aatagagggg aacggattgc ttcagcgata gcttgttctt gcgaaggtcc
    38161 atactgtatg ccgaatattt tttcgatacg ccgaatattc ttctgcactt ctttttcagg
    38221 ataatggatt tcttttttac gactgagaag gcgctggatc gaattaccga tcccccattc
    38281 agaaaagtac aaactgtttt cgtaaagatt agtctcttct tgttgtatct ttccttcttc
    38341 aacaagttgg ataattacag tagcaacctg atcaggctgg atttcaattg ggcgacttgt
    38401 ttctaaaata cggatcgttt gctgaagcaa tgtctccgcc ggtacgtatg tatctcctgt
    38461 ttgaactgca tgctgaaaaa tctgatgcaa gatagctgct cgaattcttc tcggtgaatc
    38521 tgctgcaata ccgatttgtt cggctagatt atctgcacgt ttgaacccga ttccttctat
    38581 gtcttcgact agctggtacg gattttcttc gatgatatcc aaagactcgt tcttataggc
    38641 ctggtagata ctgaaagcta actgactacc gaacccgtaa cgattcagtc ccatgatcac
    38701 ttgatccatc ccgtggttta gccgaatcgt ctcagcaagc atttctcttt ttggcttcgt
    38761 caaaccggag atttgtttta ataccgaagg atcagcaatg attttttcga tagcatcttc
    38821 tcccagcaat tctacaattt tttctgctgt tttcttcccg attcctggga atttttcgct
    38881 ggaaagatac gctacgatcc catttgcagt tgttggcttt tcttgctcat atctttcgag
    38941 tttaagctgc cttccgtatt taggatgatc cactaattct ccataaaaac gataagtctc
    39001 attttcttga atatcgccga aacttccggt gactacaatc tctttttcac ggtaatcagc
    39061 tgtggtttca ctgatgtgta ccaaaagcac tttgtaaaaa ttactaggat tttgaaaaaa
    39121 aatcgcggca actgtaccaa cgaaataagg catttcttct ggctgcatcg gaagccgcct
    39181 ccttcttcta tagtaaaggt tctggtgctg tttcagttcc taaaaaatct gtttggttcc
    39241 atactttcag ttcatatggc aataatctgc aaggctcttc agtctctaaa atcgtcaaac
    39301 tactattgaa caatccaccc atttcacgca actcacttag ctcttttcct gtcatccatt
    39361 gaatggctgc agttaaagaa gctccatgtc ctacgaataa aagtgggcca tctttatgtt
    39421 gggcaaccgc atctgctaca acagtttcga tacgtgtgat tgccgattca atcgtttctc
    39481 cgtgaaaagg agaaggatca tacagatcta aatgattacg caaattcggc agattatccg
    39541 gatatttctg gtacatttca tcgatcgact gaccttctaa agtacccagc cctagttctc
    39601 ttagcttatc tgtataaaaa atctctaccg gacgagcaag ctctttgttg ataccttctg
    39661 ctgtatcacg tgctcgcttt gctgtactgg aataaattgc tttaaaagga acatatttta
    39721 cctttttccc aaatgctttg atttcttcgt aactttgtgg caaaagcgga gaatcgccat
    39781 tgctcccttg aaaacgtctt tcctgattcc attgtgtctt cccatgtcgg gtaaaatata
    39841 gttccatacg cagcaaactc ctgtctttct tttattttac tctataataa actgtctaat
    39901 ccagcttgtc attttcatag tgatgcgtca attctaaatt agggaatcct tgctggcgaa
    39961 gtgcttcata cactatcatt gcagctgtat tcgataaatt caatgagcgg acatgctcat
    40021 cattcatagg gatgcgtaaa cacttttctt cgttttcacg cataaatgtt tctggcaaac
    40081 cggttgtttc ttttccaaat aaaaagaagt gctcttctcc atcagcaaaa ttttcgtcac
    40141 tatatacttt atgggcaaat tttgagatca aatgtagttt acgattacct aaaaatgtta
    40201 aaaaagcttc taaattactg tggtaagtaa tatttacatc attccaataa tccaaccctg
    40261 cccgttttag atgtttgtca tcagttgaaa aacctagtgg ctcgattaaa tgaagcggag
    40321 tattcgtggc tgcacacgta cgggcaatat ttccagtgtt tgcagggatt aatggttcaa
    40381 ataaaacgat atgattcatc aggatcattc ctctcatatt tatgttcgtt tctattttct
    40441 gacaatttga tttttgcata aaaaaaaacg tatcaactcg gtgtcagtca ctgcgagtcg
    40501 ttacgttagt gaagctcttc tcactaactt ttatcagaac aggaacctga taaaaaccaa
    40561 tgaaaaaaca acttgttgct aatataacac cctcaacttt tttttgcaac ctctactcat
    40621 taaaaactgc tagaaaaacg ttttctctta gttcctttta ataattctat gtcaattgta
    40681 atcttcttca aaggattctt atataagtct gctttgattc gctcttctac ctgccattct
    40741 aaaggtgaca tggccaaaag atctttttga ttttcctctg gtatctcaaa ttcataggtt
    40801 acacgctgac gttcagttaa tttcattgct tcagcaaact tggctactac acgttcattg
    40861 gaataactct gtttttcagg ttgattcgga taaaaagcct gacgtagttc ctttaaataa
    40921 ttttcttgtg gcaccacctt caacacttct ccatcttctg agagtacacg ttggaattct
    40981 tggtaatgag aaggcgaaaa gatatttaaa accgcgttga atgtttgatc agcaaacggc
    41041 aaattggtca aatcagctac acaccaaaat gcttcggcag attgttctgt agctgcgtag
    41101 actccttctt ttgaaatatc aaatccaaca gcattttgaa tctttttcaa ttttgaaagt
    41161 tcattcaaga agctaccttc tccgcagcca acatctaata gcgagacttc ttcaggtaaa
    41221 tgctgcgcaa tagtctcaat aacgggctga tacatgccag attggatcat tctacgcctt
    41281 ggaacgaaca ttccttgatc gtattctgtt ttaaccgcat gggataaaaa ataaagtgtt
    41341 ccttttttgg aaaggtcaaa acggtgatta tttttgcata tcaagccatt atcagacttt
    41401 tttacttctt cttggcaaac cggacagcgg aacagctgat gattttctgc caaaaactgg
    41461 cgtcctcggt caattttttt taacatagtt ccccctcatt tctgttttat cttaccgtta
    41521 gatgctcact ttggcaactg ctaaaaatat gatacaatgc ctagaagaaa gtgaaagata
    41581 aaggagaaga ttagatgatc aaagaatttt gcgctgaaaa ctacacatct attcctctag
    41641 caattgctag aggagccgat cggatcgaat tgtgtgacaa cttagcggtc ggtggcacaa
    41701 ccccgagtac tggtgtgatc gaagaaacgt taagttatgc tggggaaaaa gatatccccg
    41761 taatgacgat tattcgtcct agagggggcg acttcgtcta taacgatatc gaattgaaaa
    41821 tcatgcatac tgatttgatt gaagcaaaga aattagggac ggatggcgta gtatttggtt
    41881 gtcttactcc ttcaggctgg ctagacgaag aggccttaga actattgatc gaaacagcag
    41941 aagggttgca gatcaccttc catatggctt ttgacagtat tcctgaagat cggcaattcg
    42001 aagcaatcga ttggctggta gaacatggtg ttgaccgtat cttgactcat ggcggtcctg
    42061 ctgacaaaac tattgtagaa aatttcccac ggcttaaaga attgattgcc tatacagatg
    42121 gccgcttaat cattttgcct ggtggtggtg tcactgcaga gaatgcagca catgtagcta
    42181 aagaattgaa cgtaagcgaa gtacacggaa ccaaaatcgt ggatcttcag ttataatctt
    42241 ataaacaaaa aaacatttgc aacaggcata atgcttattg caaatgtttt tgttttttta
    42301 cataaaatag cccataaata aagaagcaat attgaaataa atcagtacac tcgtcaaatc
    42361 gctcaaagtc gtgataaagg gaccgcttgc tactgctgga tcaaatccta gacgatccat
    42421 tagcatcggg attaaactac ctgcaagatt cgccactgtg attgcaaata gcatagccat
    42481 ccctatgaca aaccccaaga taaaattatg tttccaaaaa cctacgatta gaaaaatcgt
    42541 gatccccgta acagctcctg tgacaacgcc ggtcaagact tcagccaaaa caagtcgaac
    42601 aaatccatga tctttctcat cagaaatcgc aagtcgacga acagccacag ctagcgactg
    42661 ggttccagca ttaccggcag ttcctgtaat cagcgaaatg aagactgcta aaatactcgc
    42721 ttcacttacc aagtcttcat agtgactgat taaagaagcg gtcgacatac ctaaaaacaa
    42781 cagagtgatc agccaaggca aacgtcgaga cgctgccttt actggatttt cattaatttc
    42841 ttccacattg accccggcaa gtcctgaata atcgctagca gcttcatcat caataacgtc
    42901 gatgatatca tcgactgtga cgattcctaa caaatgatca tcataatcgg taacaggaag
    42961 agctaagaag tcataatctc taaatgtttg tgccacatct tcttggtcgt cgcctacatg
    43021 aactgaaagc actcgttcgc tcatcagatc agaaatcatt gcgtcgtcat cattcacgat
    43081 taggtcgcgc agagaaataa cccctactag ctgaccttct tgattaacga catagatgta
    43141 atagattgtt tcagctacat ctgcttcgtt cttcagcacg tacatcgcag agcgaacagt
    43201 ttgattggct acgatcgaaa caaattcagt tgtcatgatc gcgccagccg tttcatcctc
    43261 gtaatggaga agttccttga tttcactggc atcgtctgcc gacatcaaac ttaaatattt
    43321 agcaatttgc tttttatcca gcgtattaag caagtcaaca gcattatctg tatacatctc
    43381 agaaagcata tcagctgcat agctaggacg catttcatct aggtattctt tcatatgttc
    43441 gttgtcttct tcaatcacat cgaacatatc cgctaattct ttaggcgaca gataggaata
    43501 gatcagctgc ctgttctctt ctgtaagaga ttggtagaat tgcccctgct catagatatg
    43561 catctctaaa aaactgtttc tgaatgcctg aatattattt tcccttaaat cttgagtcaa
    43621 ctgggcaaat ctttcctcta attcttgatt ttcatccaaa ccaatctcac ctcgccttat
    43681 tcttcaaacc gtctcacaac tgcctccatg tctgcggcta aaggctgctt gaactccagc
    43741 aactcttctg taaatggatg gtaaaaacgc agatcataac aatgaagggc ttgtcttttt
    43801 attcccagat ccattcgccc gccatacata tcatcaccga gcagggcaca gccaattgcg
    43861 gaaaaatgaa ctcttatttg atgtgtcctt cctgtatgca gctggatatc aactaacgcg
    43921 atttctgtta gtcgcttctc tatccagtac tcagtctgtg ccgaacgtcc ttcatcagag
    43981 accattcgct tcaatagcga agaataatct cgggcgatcg gcaaatcgat atgtccatgt
    44041 gccgataaat ctgaaagcct tccactgact aaggcctgat attttttcac aaacttcttt
    44101 tcacgaagtt gcatatctaa ttttgcgtgc gcaaatccat gctttgcaaa aagcatcaaa
    44161 ccagatgtat cccgatctag tcgcgtcacc acatggatta cttggttttc gtaaccttgt
    44221 tttttataat aagcctttac acgattagcc attgtcccat ttggatggta ttgggcagga
    44281 atagacgaaa cgcctgctgg tttatttact accagaacgt gttcatcttc atacacgata
    44341 tcgatcggtg tttcatccaa aagtacggtt tcatgttctc cttcagcagg aatcacaatg
    44401 gttaccttat catccttagc tagagaaaac aagacatttt ctacttgatc attaactttg
    44461 atttgaccgc cttgaaattt tattttcgct aacagaccct ttgagatccc tttttctttt
    44521 aaaaaatatt ttacttgctg agaatgatct ttctcataat cccatgtaaa ctgcatatca
    44581 atcctcacta ataaaggcat ctttcacacg atgccaaaaa tgcatatgtc gataagaagc
    44641 aaaatggatc cgttcttctg ctattcgata ataaattgag cggatatttg cttttgcgac
    44701 attcaattga tcaaccgtta caagataatc ttctgtattt tccaatttta tttctagcca
    44761 ttcatcatgg gcaatcacga tcggagaacc caaagtacga aacactcgat tattcagtga
    44821 agcaatctct gccaactgaa aggcattgat actaggatgg atcacagcac cgccgacact
    44881 tttattatat gctgtcgaac cagtaggcgt agaaatcgtc aatccatctc ctctaaaact
    44941 ttcaaacagc tcgtttttga tgtatacatc ggctaccatc gtacgattgg cccgtttaat
    45001 cgttgattca ttcagtgcta aaaaatgctg gtccggtgtt tcatccaaat aactgatccg
    45061 aacatccagc aagggataac taacactttg ttcccggttc gttcgtaagc tgtctactag
    45121 ttcttccagc tcgtaatccc gccaatccgt gtaaaaccct aaatgacctg tatgcacgcc
    45181 caagaaacgc acatcattca gacaatgact gtagtgatgg aacgccgaca ataatgtgcc
    45241 gtctccgccg actgagatga ccaattctgg ctcatgatca tctcgttgaa tccctgcttg
    45301 ttctaaaagt gcgttcaaac gttctgtcac ttctatagtc tttgcttctt tattatgaac
    45361 gatcgctact ttcatcccct gcctcctctc tttcgctttc tttcttcaaa agcgttcgat
    45421 agtagacatc atctgatttt cctcttccat gggagaataa ccgctgtgct tcttggattt
    45481 cttcacggat actggacatc tcttcatcca actgataggc agcttctgcc gcacgctgaa
    45541 gtcgttcact catttcttct ggaaaaacgc cttgatattt ataattcagc gaatgctcaa
    45601 tcgttgccca gaaattcatc gcaagagtcc gaatctggat ttccgccaaa attttcttct
    45661 caccgctgat cagctgtaca ggatactcga tcaccagatg ataagaacgg tagccacttg
    45721 cttttttatt tgtgatgtaa tctcgttctt ggagaacttt catgtctttt cttttacgga
    45781 tgatctcaac aacttgatgg atatcttcta caaactgaca catgatcctt aacccggcaa
    45841 tatcagacat ttcttcttct aaacgtccca ttggaatatg ccgtaattgt gattttgtta
    45901 caatactatc gactggcttg actctcccag tgacaaattc aatcgggaca tgtcgatttt
    45961 gttcacggaa ctgtttgcgg atcccccgca gtttcacttt caattctgat acagtttgtt
    46021 catatggtgc taaaaatgct tcccaatctc gttccatagc tgatacctct acttcattaa
    46081 cattcattgt ttattctacc atatatgcaa agttttttcg tactcttgat ttatgcattg
    46141 accaaaaaaa gaaaattagg caaaatagac aaaaaggagt cgatcaaatg acacaaatgc
    46201 ttgaaatcga atataaatct atgctgacaa agcaagagta cgagatattc atcgctcact
    46261 atcaattaac agataaagat ttccgtgtac agactaatat ttatttcgat accgttgatg
    46321 aacagctgaa gaaacaaaat tgtggcctac ggatccgttt cgttggtcat caagctgaat
    46381 atacattaaa aacacctgct gaaaaagggc gtctcgaaac aacggatacc tttataacag
    46441 aagaagcaga agcatttatc ttcgaaaaac gtctgcctcg ttcaggcgca gtttttcaaa
    46501 aactttctga tttgaatatc gatcctgctt cattaatcca aaccggaaag cttacaacaa
    46561 aacgggctga attccctatt gaagaaggac ttttagctat tgatgaaagc tggggagaca
    46621 atctacacga ttttgaactg gaactagagg taggggatgc atctgtggga aaaattgctt
    46681 ttataaattt tttaaaacgt ttttctttac cttatcgtcc tgctaagaac aagatccaac
    46741 gtatgcttga agctaaaaat tagaaacctt tcattttcac aaggcacttg agttcatttt
    46801 tttacttttt tctgttaagt tataggatgt gaaagaatct agtcactagt tgtatagaat
    46861 ctattttggg ggagattcat atgatcgaaa tctatttatt tgttaatcct ttaggggaat
    46921 actgtttcga aatggaacaa cagttattac aatttatcga agaagagtac gggacaccat
    46981 caaaagaaaa gatgcaattt cgctttcttc ctttggtcaa tttgcagaca attggggatg
    47041 tcatgcaaca aaaaggaatt tctcagaaca atcttgagga acgtaatcac ttattctcaa
    47101 tgacttattc tgcagcattg gactgcaaag cggcgcagtt tcaaggaaag aaaaaaggcc
    47161 gccgtttttt gatcaaattg caagaagcag tcggctgtaa tgacatacca tattctcaag
    47221 aacttgctga atccatattt gaagaaatcg gcggcgattt gaatatgttt aaagaagatc
    47281 gtcagtctga cttcgttaaa gaagtattct tttctgatca aaaaattgct cgggaaatgg
    47341 gcatcacaaa acacccttct gcagtcgttt acaattatgc ctgcgaccgt gattttggtg
    47401 ttttagtaga agtaaaaggt acaagtagcc tttggagaat cacagagctg tgtaaaacaa
    47461 cagataactc atatcaagtt tttaatggtg aacatttaca tacaaatgat tctcagttcc
    47521 attcaagaac tagccattta cacttgcttt ctaattaaaa aaatacgtcg actcccttac
    47581 aagagagccg acgtattttt ttcgtttcta ttcgagaaaa tcaaaaatag aaagccttat
    47641 aaacgatcga tcaattcttc taattcattt agacgttgtt caaacatcga cattgcatct
    47701 tcaatatatg cagcctgtgt catatcaact cctgcttttt tcattacttc cacaggataa
    47761 tcgctgttac ctgcttttag gtaagccaag tagttctcta gtgcctctgg ttcctggttt
    47821 agtattttct tcgccagtgc tgaagcagca gaaaagccag tcgagtattg gaaaacatag
    47881 taattataat aaaaatgcgg tatccgtgac cattcgaatt taatttctgg gtcttcttcg
    47941 actgctggac cataatattt tgcattcaat ttaccataac tatcacttag gtattcacta
    48001 gtcaatggca cacctttttc atcttccgta tgcatgaaat gctcgaattc agcgaattgt
    48061 gtctggcgga aaactgttcc tttaaaccca tccaagtaat ggttgagtac ataagcccgt
    48121 acacgagggt ctttttctgt ttccaataaa tactccgtta ggatattttc attcgttgtc
    48181 gaagcaattt ctgctaaaaa gatggagtag tcgccgtaca cataaggctg gttcgaacga
    48241 gtgaaataac tatgaacact atgtcccatt tcgtggacaa gcgtaaatag ctgatccagc
    48301 gtatcatgcc aattcaataa aatatatgga tttgtgtcat agcttcccga agaataagca
    48361 ccgcttcgtt tccctttatt ttcgacaaca tcgatccaac gttcagagaa tgctttttct
    48421 acgatggcca tgtattcttc acccattggt ttcagtgctt ctaaagcttt ttcttttgct
    48481 tcttcgtacg taaaggtaat tggagcttcc cctaagaccg gtgtataaag atcatacatg
    48541 tgcagttttt ccacttctaa taaacgtttc cgtaattcca tgtatcgatg taacaaaggc
    48601 aaatgcttgt ttaccacgtc taccaaagta tcgtatacac tttcaggaat atgattattg
    48661 ctcaaagacg cttctctggc agagctgtaa ttacggactt tcgctttaaa attatgtcct
    48721 tttatatgtg tgcctaaagt agaagcaaat gtatttctaa attgttcgta aacactgtac
    48781 aatcccttaa atgctgcttc gcgcaccctt cgatccgtgc tttctagcaa ctgaccatac
    48841 acgccatgag ataattggac tatttcacca ttttctcctt caatcgttgg aaaaactaga
    48901 tctgcattat tcaaaacagc aaatgtatca cttgaagcat caaagatttc acctgctcca
    48961 gcaagaagag attcctgctc agcagataag acatgggctc gattatctac tatttgctgg
    49021 atataatggc gatagacttc caattttggt tcttctttga aatattgcca aatctggtca
    49081 tctgacaatt gcaatatttc tggttcaaac caggaaacgg cttcactaac tttcgaaaac
    49141 agactgcttg ctcttgcata aagcgcttgg taatctgtat ttccagtgtc ttgatcgttt
    49201 ttaaggtgcg catatacata aatgacttca gtttggcgat aaaccctcaa tacgaattca
    49261 atcgcattta aaaattgaga agcgccttga tcaagtgttc ctttgtgttt ttcagattgt
    49321 tttaactctt ctgataattc caaatatttc tcatcaaact cttggtcgct tgaaaagatc
    49381 ttggtcaagt cccaagttaa attttcaggc aattcttctc gttttggcaa ctgctttact
    49441 tccatgaaat ccactccttt tcttccctta gctctatcta tcaaaataga tcatagtcta
    49501 acatccacat gtcacagcat ctaacttttc tactcattca tgtgtctgct tccatcaatt
    49561 ttagcataac taaatcttgt tttacataga aaaccgattc ctagcctatt tttatactta
    49621 ctattttttc aaagaagaca agtgtatgca ttcgttaaaa aactgctgat agaccatctc
    49681 ttcatcaatc ataggaaaaa gccattccct tttatgttct tttatttggc aaagccatga
    49741 gttgaaatca ggaactttct ggttttccaa ggcttcttcg tacaacattc gataaagaaa
    49801 aacctgttct tgtaaataaa agaaaaaccg acttttttga tagatccacg atgatagatg
    49861 caaaagatgt ttatgacgca agtagcattg ttcttgtatg gatactgtat tcttttgctt
    49921 gtgaataagt tggcgtgtca gccatttctt cccatcttca ctcacttttt ctattttttt
    49981 catttggttc attttaattt cgttactagt gaatatttct ttaaggtccc tactagaaaa
    50041 agtccagcag ctactgcaaa aggaaatatg ccctgataca gtctctgtaa tatgatgata
    50101 caaataaagc ttttgtttct tgagatcagc tttccagcaa tgcactcctc tttttctatt
    50161 atagtagcag caactttttt ctatcaatga gaattgcgat tgtccaagaa aagaattacc
    50221 aagtatccac catacttggt aactgttatt caaataattt tgggttcttt caataaatct
    50281 ctggtgagac agtctagaac attggatttc tattgctaga ttcgccaaca aaatatccgg
    50341 cctttgccgc aacctcggca aataggcttc caaaaatact ggttctactg actgtcccag
    50401 ccaatccatg aagcattcct ttaactgtaa gtgctctttt gattctcttt cactaaagac
    50461 cgaacagtta tctgattttt gatgggcaaa atgagcacat tttttctttc cctttttcaa
    50521 aatcactctt tttcgacagc cacgacaata atatttcttt cctttttctg catgtttagc
    50581 aaaacagcac ttattatatt cattcattgc aagcagcatt tttctccctc tgaacttatt
    50641 tacgtctaag aatttattta cacgaaaaaa gagggcgtga aaaagctatt ctgcatcaag
    50701 taataagaac agctaaacaa aaatagttcc caatattttt cgtttggttg ggcttgttac
    50761 cgcagatgca agcttttgaa cacatttata cgaaaaaaag actgcgacac aatttttgtc
    50821 acagtctctt actataatgc tgatttatac aaaataaaat ttcgttcgct cgatagcatt
    50881 acgcgccatc agacattgtc catattcatt caatgtttct ggtgtacgat cgctcttttc
    50941 agcaaactcc aaaattcgag caatctgatt ctctacatcc gcttggttga gattttccat
    51001 ccagaagtga acgactaggt aatagcgctc ttcgaataaa tacaaatcag tccaagcaga
    51061 ctctagttcg acatctgttg caagttggat cacttgttca aaatcttcaa aagcaaaagt
    51121 tgcttgtctt tcctgttctt ctagggcgct tccagataat tcttcctccg gatcgctgcc
    51181 aataatctgc tgacgaagaa agtcactcac atcttctgaa tcaacagtag aatcttctga
    51241 ttgagaaatt tcttcatccg gaagattttt actgataaac agttctaatc cgtctccttt
    51301 tggcaaaact tggaaagtga cagcttcgct tcctcggaac tcctcttcga tatcaacttc
    51361 ttccaaaata ctataaaaga aattttcgat ttctttgtga ttgccaagta aatccaaaaa
    51421 tgaaacgccc cgttcctcta aatcttcatg accaattagt acacggatcg tattttcatt
    51481 aatatgttcc atttccattt tgctacacct cactttgcca caatgtttaa tatagattca
    51541 ttgtagcgaa atcagcttaa atgtaaagaa aaaaaatctc gctgttaagc gaatttaata
    51601 aaaccagcgg atttttcaac ctttcaagct atcttattta actataacat ttttggcaga
    51661 cgaatactat atagccgact tttcagctaa ttttccctct ctatttttcg tgcaatcatc
    51721 atcttctact acctttttat acatctaatc aagaatactt tttgtaaaaa caaaaaaact
    51781 tggaaaaact atctttgtaa tagt
//
LOCUS       DACEKH010000009        51287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEKH010000009 DACEKH010000000
VERSION     DACEKH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.009"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            330..1175
                     /locus_tag="IW662_000464"
     CDS             330..1175
                     /locus_tag="IW662_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0865314.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="IW662_000465"
     CDS             1456..2676
                     /locus_tag="IW662_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ0865315.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHISQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="IW662_000466"
     CDS             2673..5393
                     /locus_tag="IW662_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0865316.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="IW662_000467"
     CDS             5377..6321
                     /locus_tag="IW662_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0865317.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="IW662_000468"
     CDS             complement(6390..7400)
                     /locus_tag="IW662_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0865318.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="IW662_000469"
     CDS             complement(7580..7924)
                     /locus_tag="IW662_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0865319.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="IW662_000470"
     CDS             complement(7917..8351)
                     /locus_tag="IW662_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ0865320.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="IW662_000471"
     CDS             8489..9226
                     /locus_tag="IW662_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865321.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="IW662_000472"
     CDS             9219..10430
                     /locus_tag="IW662_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0865322.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="IW662_000473"
     CDS             10637..11416
                     /locus_tag="IW662_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ0865323.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="IW662_000474"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="IW662_000474"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ0865324.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IW662_000475"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IW662_000475"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ0865325.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="IW662_000476"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="IW662_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0865326.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="IW662_000477"
     CDS             15018..16067
                     /locus_tag="IW662_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ0865327.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="IW662_000478"
     CDS             16064..16342
                     /locus_tag="IW662_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ0865328.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="IW662_000479"
     CDS             16317..16787
                     /locus_tag="IW662_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ0865329.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="IW662_000480"
     CDS             16804..17106
                     /locus_tag="IW662_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865330.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="IW662_000481"
     CDS             17123..17494
                     /locus_tag="IW662_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ0865331.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="IW662_000482"
     CDS             17466..17825
                     /locus_tag="IW662_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865332.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="IW662_000483"
     CDS             17997..19004
                     /locus_tag="IW662_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0865333.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="IW662_000484"
     CDS             19029..20213
                     /locus_tag="IW662_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ0865334.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="IW662_000485"
     CDS             complement(20650..22233)
                     /locus_tag="IW662_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0865335.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="IW662_000486"
     CDS             complement(22811..22954)
                     /locus_tag="IW662_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ0865336.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="IW662_000487"
     CDS             complement(23176..23655)
                     /locus_tag="IW662_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0865337.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="IW662_000488"
     CDS             complement(23728..24012)
                     /locus_tag="IW662_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865338.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="IW662_000489"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IW662_000489"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="IW662_000490"
     CDS             complement(24356..25642)
                     /locus_tag="IW662_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ0865339.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="IW662_000491"
     CDS             25780..26451
                     /locus_tag="IW662_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ0865340.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="IW662_000492"
     CDS             26417..26905
                     /locus_tag="IW662_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ0865341.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="IW662_000493"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="IW662_000493"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ0865342.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="IW662_000494"
     CDS             27866..28630
                     /locus_tag="IW662_000494"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ0865343.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="IW662_000495"
     CDS             28863..31076
                     /locus_tag="IW662_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ0865344.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /locus_tag="IW662_000496"
     CDS             31087..31533
                     /locus_tag="IW662_000496"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ0865345.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="IW662_000497"
     CDS             31704..32963
                     /locus_tag="IW662_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ0865346.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="IW662_000498"
     CDS             complement(33052..33999)
                     /locus_tag="IW662_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0865347.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /locus_tag="IW662_000499"
     CDS             34393..35685
                     /locus_tag="IW662_000499"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ0865348.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="IW662_000500"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="IW662_000500"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ0865349.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="IW662_000501"
     CDS             37578..38453
                     /locus_tag="IW662_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0865350.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="IW662_000502"
     CDS             38719..40602
                     /locus_tag="IW662_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0865351.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="IW662_000503"
     CDS             complement(40677..40961)
                     /locus_tag="IW662_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0865352.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="IW662_000504"
     CDS             complement(40951..41685)
                     /locus_tag="IW662_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ0865353.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="IW662_000505"
     CDS             41814..42437
                     /locus_tag="IW662_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ0865354.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="IW662_000506"
     CDS             42629..43750
                     /locus_tag="IW662_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ0865355.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="IW662_000507"
     CDS             43757..46885
                     /locus_tag="IW662_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ0865356.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="IW662_000508"
     CDS             46904..50098
                     /locus_tag="IW662_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0865357.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="IW662_000509"
     CDS             complement(50244..50951)
                     /locus_tag="IW662_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0865358.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16790 a         8953 c        10642 g        14902 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattagtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgcaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttccgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    51181 taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac
    51241 tattcaaaca taaaataacg ttatttgatt tatttgttta tagtaaa
//
LOCUS       DACEKH010000010        43679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEKH010000010 DACEKH010000000
VERSION     DACEKH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.010"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(105..629)
                     /locus_tag="IW662_000510"
     CDS             complement(105..629)
                     /locus_tag="IW662_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0865359.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IW662_000511"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IW662_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ0865360.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IW662_000512"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IW662_000512"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ0865361.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IW662_000513"
     CDS             complement(2351..3031)
                     /locus_tag="IW662_000513"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0865362.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IW662_000514"
     CDS             3157..4026
                     /locus_tag="IW662_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0865363.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IW662_000515"
     CDS             4052..4567
                     /locus_tag="IW662_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865364.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IW662_000516"
     CDS             complement(4614..5177)
                     /locus_tag="IW662_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0865365.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IW662_000517"
     CDS             complement(5232..5411)
                     /locus_tag="IW662_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865366.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IW662_000518"
     CDS             complement(5488..5997)
                     /locus_tag="IW662_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865367.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IW662_000519"
     CDS             complement(6093..6473)
                     /locus_tag="IW662_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0865368.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="IW662_000520"
     CDS             6803..7552
                     /locus_tag="IW662_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ0865369.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="IW662_000521"
     CDS             complement(7736..9013)
                     /locus_tag="IW662_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865370.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IW662_000522"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IW662_000522"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0865371.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="IW662_000523"
     CDS             complement(9935..10879)
                     /locus_tag="IW662_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0865372.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="IW662_000524"
     CDS             complement(10881..11567)
                     /locus_tag="IW662_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0865373.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="IW662_000525"
     CDS             12041..12361
                     /locus_tag="IW662_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0865374.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="IW662_000526"
     CDS             12515..13420
                     /locus_tag="IW662_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0865375.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="IW662_000527"
     CDS             13417..14190
                     /locus_tag="IW662_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0865376.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="IW662_000528"
     CDS             complement(14371..15795)
                     /locus_tag="IW662_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0865377.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="IW662_000529"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="IW662_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0865378.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="IW662_000530"
     CDS             complement(16517..18499)
                     /locus_tag="IW662_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0865379.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IW662_000531"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IW662_000531"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0865380.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="IW662_000532"
     CDS             complement(19412..20473)
                     /locus_tag="IW662_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0865381.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21211)
                     /locus_tag="IW662_000533"
     CDS             complement(20684..21211)
                     /locus_tag="IW662_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0865382.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /locus_tag="IW662_000534"
     CDS             complement(21278..22780)
                     /locus_tag="IW662_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0865383.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="IW662_000535"
     CDS             complement(23038..24159)
                     /locus_tag="IW662_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0865384.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..25226
                     /locus_tag="IW662_000536"
     CDS             24285..25226
                     /locus_tag="IW662_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0865385.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25376..26272)
                     /locus_tag="IW662_000537"
     CDS             complement(25376..26272)
                     /locus_tag="IW662_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0865386.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26541..27881
                     /locus_tag="IW662_000538"
     CDS             26541..27881
                     /locus_tag="IW662_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0865387.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28099..28542
                     /locus_tag="IW662_000539"
     CDS             28099..28542
                     /locus_tag="IW662_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0865388.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="IW662_000540"
     CDS             complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="IW662_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0865389.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30867..32090)
                     /locus_tag="IW662_000541"
     CDS             complement(30867..32090)
                     /locus_tag="IW662_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ0865390.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32121..32960)
                     /locus_tag="IW662_000542"
     CDS             complement(32121..32960)
                     /locus_tag="IW662_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ0865391.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32977..33657)
                     /locus_tag="IW662_000543"
     CDS             complement(32977..33657)
                     /locus_tag="IW662_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0865392.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33659..34732)
                     /locus_tag="IW662_000544"
     CDS             complement(33659..34732)
                     /locus_tag="IW662_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0865393.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(35379..36050)
                     /locus_tag="IW662_000545"
     CDS             complement(35379..36050)
                     /locus_tag="IW662_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0865394.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="IW662_000546"
     CDS             complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="IW662_000546"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ0865395.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39474..39911
                     /gene="ndk"
                     /locus_tag="IW662_000547"
     CDS             39474..39911
                     /gene="ndk"
                     /locus_tag="IW662_000547"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ0865396.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39933..40799)
                     /locus_tag="IW662_000548"
     CDS             complement(39933..40799)
                     /locus_tag="IW662_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0865397.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40959..43052)
                     /locus_tag="IW662_000549"
     CDS             complement(40959..43052)
                     /locus_tag="IW662_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0865398.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        12612 a         9150 c         7717 g        14200 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgttttt
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    25081 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    25141 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    25201 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    25261 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    25321 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    25381 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    25441 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    25501 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    25561 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    25621 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    25681 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    25741 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    25801 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    25861 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    25921 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    25981 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    26041 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    26101 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    26161 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    26221 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    26281 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    26341 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    26401 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    26461 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    26521 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    26581 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    26641 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    26701 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    26761 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    26821 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    26881 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    26941 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    27001 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    27061 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    27121 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    27181 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    27241 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    27301 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    27361 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    27421 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    27481 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    27541 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    27601 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    27661 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    27721 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    27781 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    27841 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    27901 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    27961 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    28021 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    28081 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    28141 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    28201 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    28261 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    28321 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    28381 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    28441 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    28501 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    28561 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    28621 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    28681 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    28741 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    28801 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    28861 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    28921 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    28981 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    29041 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    29101 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    29161 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    29221 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    29281 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    29341 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    29401 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    29461 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    29521 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
    29581 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    29641 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
    29701 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    29761 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    29821 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
    29881 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    29941 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    30001 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
    30061 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    30121 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    30181 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
    30241 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    30301 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    30361 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    30421 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    30481 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    30541 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    30601 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    30661 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    30721 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
    30781 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    30841 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
    30901 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    30961 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    31021 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    31081 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    31141 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    31201 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    31261 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    31321 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    31381 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    31441 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    31501 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    31561 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    31621 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    31681 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    31741 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    31801 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
    31861 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    31921 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    31981 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    32041 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    32101 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    32161 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    32221 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    32281 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    32341 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    32401 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    32461 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    32521 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    32581 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    32641 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    32701 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
    32761 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    32821 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
    32881 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    32941 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    33001 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    33061 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    33121 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    33181 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
    33241 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    33301 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    33361 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    33421 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    33481 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
    33541 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    33601 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    33661 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
    33721 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
    33781 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    33841 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    33901 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    33961 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    34021 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
    34081 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    34141 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    34201 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    34261 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    34321 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    34381 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    34441 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    34501 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
    34561 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    34621 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    34681 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    34741 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    34801 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    34861 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    34921 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    34981 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    35041 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
    35101 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
    35161 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
    35221 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
    35281 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
    35341 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
    35401 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
    35461 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
    35521 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
    35581 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
    35641 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
    35701 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
    35761 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
    35821 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
    35881 gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
    35941 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
    36001 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
    36061 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
    36121 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
    36181 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
    36241 gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa
    36301 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
    36361 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
    36421 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    36481 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    36541 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    36601 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    36661 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    36721 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    36781 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    36841 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    36901 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    36961 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    37021 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    37081 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    37141 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    37201 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    37261 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    37321 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    37381 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    37441 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    37501 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    37561 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    37621 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    37681 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    37741 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    37801 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    37861 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    37921 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    37981 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    38041 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    38101 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag acaagatttt
    38161 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    38221 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    38281 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    38341 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    38401 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    38461 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    38521 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    38581 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    38641 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    38701 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    38761 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    38821 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    38881 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    38941 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    39001 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    39061 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    39121 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    39181 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    39241 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    39301 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    39361 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    39421 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    39481 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    39541 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    39601 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    39661 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    39721 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    39781 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    39841 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    39901 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    39961 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    40021 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    40081 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt
    40141 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    40201 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    40261 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    40321 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    40381 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    40441 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    40501 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    40561 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    40621 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    40681 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    40741 tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata
    40801 atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga
    40861 aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat
    40921 tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt
    40981 tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa
    41041 aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca
    41101 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    41161 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    41221 tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    41281 ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga
    41341 aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct
    41401 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat
    41461 tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc
    41521 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa
    41581 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    41641 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    41701 tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    41761 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    41821 aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat
    41881 atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca
    41941 taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa
    42001 acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg
    42061 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    42121 atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg
    42181 atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc
    42241 ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct
    42301 ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa
    42361 acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    42421 attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca
    42481 aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa
    42541 ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg
    42601 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc
    42661 cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac
    42721 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    42781 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac
    42841 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt
    42901 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    42961 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    43021 cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt
    43081 ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt
    43141 ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag
    43201 cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa
    43261 aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct
    43321 ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg
    43381 ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt
    43441 gttccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat
    43501 cagctgaccc tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg
    43561 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta
    43621 ttttttgaaa ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgata
//
LOCUS       DACEKH010000011        42230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEKH010000011 DACEKH010000000
VERSION     DACEKH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.011"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            546..2156
                     /locus_tag="IW662_000550"
     CDS             546..2156
                     /locus_tag="IW662_000550"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ0865399.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2448..3317
                     /locus_tag="IW662_000551"
     CDS             2448..3317
                     /locus_tag="IW662_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ0865400.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3482..4753
                     /locus_tag="IW662_000552"
     CDS             3482..4753
                     /locus_tag="IW662_000552"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0865401.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4750..6045
                     /locus_tag="IW662_000553"
     CDS             4750..6045
                     /locus_tag="IW662_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ0865402.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            6144..6407
                     /locus_tag="IW662_000554"
     CDS             6144..6407
                     /locus_tag="IW662_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ0865403.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6852..7301
                     /locus_tag="IW662_000555"
     CDS             6852..7301
                     /locus_tag="IW662_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0865404.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7358..8776
                     /locus_tag="IW662_000556"
     CDS             7358..8776
                     /locus_tag="IW662_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0865405.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRMIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8776..9636
                     /locus_tag="IW662_000557"
     CDS             8776..9636
                     /locus_tag="IW662_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ0865406.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9810..10583
                     /locus_tag="IW662_000558"
     CDS             9810..10583
                     /locus_tag="IW662_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0865407.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10730..11095
                     /locus_tag="IW662_000559"
     CDS             10730..11095
                     /locus_tag="IW662_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ0865408.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11243..12451)
                     /locus_tag="IW662_000560"
     CDS             complement(11243..12451)
                     /locus_tag="IW662_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0865409.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12470..12748)
                     /locus_tag="IW662_000561"
     CDS             complement(12470..12748)
                     /locus_tag="IW662_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865410.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            12941..13597
                     /locus_tag="IW662_000562"
     CDS             12941..13597
                     /locus_tag="IW662_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0865411.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(14039..14836)
                     /locus_tag="IW662_000563"
     CDS             complement(14039..14836)
                     /locus_tag="IW662_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ0865412.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14833..16017)
                     /locus_tag="IW662_000564"
     CDS             complement(14833..16017)
                     /locus_tag="IW662_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0865413.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(16042..17289)
                     /locus_tag="IW662_000565"
     CDS             complement(16042..17289)
                     /locus_tag="IW662_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ0865414.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17504..19249
                     /gene="dnaX"
                     /locus_tag="IW662_000566"
     CDS             17504..19249
                     /gene="dnaX"
                     /locus_tag="IW662_000566"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ0865415.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19269..19583
                     /locus_tag="IW662_000567"
     CDS             19269..19583
                     /locus_tag="IW662_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ0865416.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19608..20204
                     /gene="recR"
                     /locus_tag="IW662_000568"
     CDS             19608..20204
                     /gene="recR"
                     /locus_tag="IW662_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ0865417.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20268..20912
                     /locus_tag="IW662_000569"
     CDS             20268..20912
                     /locus_tag="IW662_000569"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ0865418.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            20927..21256
                     /locus_tag="IW662_000570"
     CDS             20927..21256
                     /locus_tag="IW662_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865419.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21270..22208
                     /gene="holB"
                     /locus_tag="IW662_000571"
     CDS             21270..22208
                     /gene="holB"
                     /locus_tag="IW662_000571"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ0865420.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22244..23068
                     /locus_tag="IW662_000572"
     CDS             22244..23068
                     /locus_tag="IW662_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ0865421.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            23061..23408
                     /locus_tag="IW662_000573"
     CDS             23061..23408
                     /locus_tag="IW662_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ0865422.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23477..24349
                     /gene="rsmI"
                     /locus_tag="IW662_000574"
     CDS             23477..24349
                     /gene="rsmI"
                     /locus_tag="IW662_000574"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ0865423.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24458..25579
                     /gene="dinB"
                     /locus_tag="IW662_000575"
     CDS             24458..25579
                     /gene="dinB"
                     /locus_tag="IW662_000575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ0865424.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25633..26235)
                     /gene="nrdG"
                     /locus_tag="IW662_000576"
     CDS             complement(25633..26235)
                     /gene="nrdG"
                     /locus_tag="IW662_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ0865425.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26550..28739)
                     /gene="nrdD"
                     /locus_tag="IW662_000577"
     CDS             complement(26550..28739)
                     /gene="nrdD"
                     /locus_tag="IW662_000577"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ0865426.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            29065..29832
                     /locus_tag="IW662_000578"
     CDS             29065..29832
                     /locus_tag="IW662_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865427.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            29822..31864
                     /locus_tag="IW662_000579"
     CDS             29822..31864
                     /locus_tag="IW662_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0865428.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            32145..32294
                     /locus_tag="IW662_000580"
     CDS             32145..32294
                     /locus_tag="IW662_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ0865429.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            32305..33819
                     /gene="dltA"
                     /locus_tag="IW662_000581"
     CDS             32305..33819
                     /gene="dltA"
                     /locus_tag="IW662_000581"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ0865430.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            33816..35021
                     /gene="dltB"
                     /locus_tag="IW662_000582"
     CDS             33816..35021
                     /gene="dltB"
                     /locus_tag="IW662_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ0865431.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            35066..35299
                     /gene="dltC"
                     /locus_tag="IW662_000583"
     CDS             35066..35299
                     /gene="dltC"
                     /locus_tag="IW662_000583"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ0865432.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            35302..36570
                     /gene="dltD"
                     /locus_tag="IW662_000584"
     CDS             35302..36570
                     /gene="dltD"
                     /locus_tag="IW662_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0865433.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            36711..37793
                     /locus_tag="IW662_000585"
     CDS             36711..37793
                     /locus_tag="IW662_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ0865434.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            37793..38596
                     /locus_tag="IW662_000586"
     CDS             37793..38596
                     /locus_tag="IW662_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0865435.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            38666..39406
                     /locus_tag="IW662_000587"
     CDS             38666..39406
                     /locus_tag="IW662_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0865436.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            39417..40421
                     /locus_tag="IW662_000588"
     CDS             39417..40421
                     /locus_tag="IW662_000588"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ0865437.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(40466..42229)
                     /locus_tag="IW662_000589"
     CDS             complement(40466..42229)
                     /locus_tag="IW662_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865438.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT        13575 a         7390 c         8771 g        12494 t
ORIGIN      
        1 atattattta atagaaaaga tatgccaaaa atccgagaac tgtggactac gttttttacg
       61 taaaggaggt aaataagggt gaaagaaaaa gattgtttct acgataagct atataaactg
      121 ttgctgcaag taggaaaaag tagttctttg ttaggtggga tattatctat cataatgttt
      181 gttttatttt tttatctatt atttcactta aaatttttca gtaacgattt tctgatttgc
      241 ttaggtttaa cgttagctat gtttatatca agcttattat gtgggttaaa ttttttttgc
      301 actcataaag gacttaagaa ataatttctc aaaggatatc gaaaaagaaa aaattgtaag
      361 cggatagtat tcatttgagt acttgacttt cccttagtca gcttgtatta tacaccttgg
      421 gcaccctttt agagggtcag aagtttacag aaatttcggc tccctattct tattgaagaa
      481 tagggagctt tgtttattgt ctgagacttt tcctgcacat aaattaaaaa ggagtttttg
      541 aacttatgac aaaatatatt tttgtgaccg gcggtgtcgt ttcatcaatc ggaaaaggga
      601 ttgttgcagc ttctttagga cgcttattga aaaaccgcgg cttaaaagta actatccaaa
      661 aatttgatcc atacatcaac gtggatccgg ggacgatgag cccatatcag cacggagaag
      721 tattcgtaac agatgatggt gctgaaacag atttggatct gggccactac gaacgtttta
      781 ttgacatcaa tttgaacaaa tactccaacg tgacgacagg aaaaatctat tctgaagttt
      841 tgcgtaaaga acgtaagggc gaatacttgg gagcaactgt tcaagttatt cctcatatta
      901 caaatgagat caaagaaaaa atcatgcgag cagctaaaat gactgattca gatgtgatca
      961 ttacagaagt agggggaaca gtaggggata tagaatcttt gccattcctt gaagcacttc
     1021 gtcaaatgaa agcagatgtc ggtacagata atgttatgta tatccataca acattgattc
     1081 cttacttgaa agctgctgga gaaatgaaaa ctaaaccaac gcagcacagt gtaaaagagc
     1141 tacgtggttt aggtatccaa ccgaatattt tggtggtacg tacagagcaa cctgtttctc
     1201 aaagcgtgaa aaacaaattg gcacaatttt gtgatgtcga accagaagca gttatcgagt
     1261 cgccagatgt agatacactt tactccatcc cattagtttt acaagcacaa ggaatggatc
     1321 aaattgtttg cgaccattta aaactagata caccagaagc agacatgaca gaatggaaag
     1381 aattagaaga acgtgtcttg aacttgaaga aaaaagtacg gattgcgctt gtcggtaaat
     1441 atgtcgagtt accagatgca tatatctcag tagttgaagc attgaaacat tctggtttcg
     1501 cctataattc agacatcgag atcgattgga tcaaagcaca agaattaacg agagaaaatg
     1561 tagaagaacg tttgaaagac gcagatggaa tcttggttcc tggtggattt ggtgatcgcg
     1621 gagtagaagg gaaaatcgaa gcgattcgtt atgctcgcga aaatgacgtt ccgtttttag
     1681 gtatttgtct tggtatgcag atggcttgtg tcgaatttgc acgtaacgtc gttggattag
     1741 aagatgcgtc atcagctgaa acgaatccag atacagcaaa caatattatt gatttaatgg
     1801 ctgatcagga aaacatcgag aatttgggtg gaactttacg ccttggatta tacccatgta
     1861 aaatcaaaaa aggaacgaag acggctgaag catatgatgg agccgatgtt gtgcaagaac
     1921 gtcaccgtca ccgctatgag ttcaataaca aatatcgtca attgtttgaa gaacaagggc
     1981 tagtcttttc aggtgtctca ccagataacc gtttagtaga aatcgtagag ctgccagaga
     2041 aaaaattctt tgtgggatgc caattccacc cagaattgat ttctcgtcca aatcgcccgc
     2101 aaaaattaat caaagcattt gtcggagcat cattagcaga tcacgaaaca aaataatttt
     2161 tgaacttgat agaaagagaa aagtagcaag agaaggggat aagcaatttt cttgtcactt
     2221 ttctcttttt ctgatttgtt attatttgta ggaaaaaaga tagaaagcaa aagacttttt
     2281 tcgattaaag cgctattgga tattttattt tttgcaaaaa acttcttgaa aattttcatt
     2341 ttcattgatt ttagtagaaa actaccgaaa tgtttggtaa aatagggtcg ttggttaaga
     2401 attgtcttaa caaatttagt tttacaaaac ttaggaggaa ttttattatg ccagtagtat
     2461 caggagctga atttttaaaa gctgctcgta aaggcggtta cgctgtcggc ggattcaaca
     2521 caaacaactt ggaatggact caagcaatcc ttgaagcggc tgaagcaaaa caagcaccag
     2581 tacttatcca aacatcaatg ggtgcagcta aatacatggg tggttacaaa atctgtaaag
     2641 atttaatcac tgatttagtt gaagcaatga acatcactgt tccagttgct atccacttag
     2701 accatggtga ttacgaagct gctttggaat gtatcgaatt aggctatact tcagtaatgt
     2761 tcgatggttc acatctacca tttgaagaaa acttgaaatt agctaaagac gttgttgaaa
     2821 aagcacatgc aaaaggtatc tcagttgaat gtgaagttgg ttcaatcggc ggagaagaag
     2881 acggaatcat cggtaacggc gaattagctg atattgaaga atgcaaacaa atggttgcta
     2941 caggtatcga ctacttagct tgcggtatcg gtaacatcca tggtcaatat ccagaaaact
     3001 ggaaaggatt agcttttgac cacttacaag caattgctga tgctgtaggt ccagacatgc
     3061 ctttagtatt acatggcgga tcaggtattc ctcaagatca aatcgaaaaa gctatctcaa
     3121 tgggtatctc aaaagtaaat gttaacactg aattccaatt atcatttgct gcagctactc
     3181 gtaaatatat cgaagctggt aaagataaag aaggtaaagg attcgaccct cgtaaattat
     3241 tagctccagg taaagcagca atcgttgaag atgctaaatc tcatatcgac tggttcggtt
     3301 cagctaacaa agcgtaagca tccacataac agatacgata tataggaatg gagaacgact
     3361 tttgaagtcg ctctccattc ttttttactt aaaacggcag accatgctcg tttctttttt
     3421 ttatctatga taaaatacaa gcatatccat gaaaaaagat taataaacaa ggtggaaaca
     3481 gatgaaaaaa atcgtaatca aaggaaaccg acctttagct ggtgaagtaa cgatcagcgg
     3541 tgcaaaaaat agtgcagtcg cattgattcc agctgcaatc ttagctgatt ccccagtgat
     3601 cttggaaggt gtgccggaca tacaagatgt tcattcatta attgaaattt tagagatcat
     3661 gggcgcaact gtccattttt ccaacaacat actagaaatc gatcctcgag aagtcgtatc
     3721 gattccgatg ccgcacggaa aaatcaatag tttgcgtgct tcttattatt ttatgggaac
     3781 actgcttggt aaatttggcg aagcggtagt aggcctgcca ggtggctgtt atttaggccc
     3841 tagaccgatt gacctacacg tcaaaggatt tgaagctcta ggtgctgaag tgacaaatga
     3901 acatggtgcc atgtatttac ggacagaaaa taaaggattg cgtggcaatc ggattttcat
     3961 ggacgtggtt tcagtcggag caacgatcaa tgtgatgtta gctgctgtaa aagcaaaagg
     4021 acagacagta attgaaaacg ctgcacgaga accggaaatc atagatgtgg caactttact
     4081 gaataatatg ggggcaaaag ttcgaggagc gggtacagat gtgatccgta tcgaaggagt
     4141 cgaaacatta catggctgtc ggcatttcat gattcctgac cgcatcgaag caggaaccta
     4201 tctagcatta gcagctgcag taggaaatgg tatcaaagtg aaaaatgtga tctttgaaca
     4261 tttggaaagc tttatagcaa aattgcagga aattggtgtc catatgaaaa tcagcgaaga
     4321 tgaaatcgaa gtttacccct cgaaagaatt aaaacccgca aacatcatga cctatcctta
     4381 tccaggattt gccacagatc tgcagcagcc acttactgcg ttactattga tgactacagg
     4441 cacttcagaa atcatcgata cgatctatgc gaaacgtgtg aaccatgtgc cggaactagc
     4501 acgaatgggc gctgatatta ccgtggaagg aaatatgatc atcgtgaatg gtccaaataa
     4561 acttcacggc acagaagtcg tggcttctga tcttcgggca ggtgcttgtt tagtgattgc
     4621 tggactattg gcagaaggga caacgacgat ttacaatgta gattatatct tacgcggtta
     4681 tgatcatatt atcgagaaac taacagcctt aggggcatca attgaaatga tagaggaagt
     4741 gggagctgaa tgagcgatta tttgacaatg gcagaactag agaacaaaac gttaaaagaa
     4801 gtttatagtt atgccaaaga atttaaaatt ccttattaca gtaagatgaa taaaaaggaa
     4861 ttatctctag ctgtgattcg tgcacaagca gagaaacaag gattttatta tatggaagga
     4921 atattggata tcgttggtca agatggttac ggttttttac ggccaatcaa ttatggacca
     4981 agtgcagaag atatctatat ttctgcctca caaatcagac gattcagttt gagaaatggg
     5041 gacaaagtgg caggaaaagc ccgtccacct aaagaatcgg aacgttatta tggtttgatg
     5101 cacgtggaaa gcgttaatgg aaaagatccg gaagaagcaa aagaacgtcc gcattttcca
     5161 gccttaacac ctctttatcc gcaaagacag cttcaactgg aaacaacacc gcaaagacta
     5221 tctacccgaa tgatcgatat cttttctcca atcggttttg gtcagcgtgg attgatcgtc
     5281 gcaccgccaa aagcagggaa aacttctgta ttaaaagaaa tcgcaaatgg tatcaccgaa
     5341 aactatccgg aagtagaatt aatcgttcta ctgattgatg aacgaccgga agaagtaacg
     5401 gatctagaac gaagcgtgaa aggcgacgtg gtatcttcta catttgatct gcagccgcaa
     5461 aaccatactc gagtttctga attagtattg gaacgcgcaa tgcgtttagt cgaagataaa
     5521 cgagatgtcg ttatcttgat ggatagtatt actagacttg ctcgggccta taacttagtc
     5581 gttccaccaa gcggacgtac attgagtggc gggattgacc cagccgcatt gtataaacct
     5641 aagaaatttt tcggggcagc aagaaacatc gaagaaggtg gcagtttgac gattttagcg
     5701 accgctttag tcgatacagg aagccgtatg gatgacgtga tctatgaaga attcaaagga
     5761 actggaaatc tagaattaca actttcacgt gaattatctg aacgtcggat cttcccagcc
     5821 atcgatatca aaaaatcagg tacccgaaaa gaagaattat tgatgccaag tgaagaatta
     5881 gaagaaattt ggaaacttcg aaaaaatatg actggagatt cattagaata tacggaacaa
     5941 ctgatccgct ttttgcgtaa aacaaaaaac aatcagcaat ttttcaaaga atttcaagat
     6001 gtctcctttg gtaaaaaaaa tccgaccaga aatctgaaaa gataattgca accttgactg
     6061 aaacatgtta agatgttaat gttgtaaatc gacaaatcaa ctctggttca gcaaatggag
     6121 cagggcaaag gagcgaaaaa atcatgaaag aaaacatcca tccagattac cacccagtag
     6181 tattcatgga ttcaacaact ggtttcaaat tcttgtcagg ttcaacaaaa acttcacaag
     6241 aaacagttga atgggaagac ggtaatacat acccagtcat ccgtgtcgaa gtaacttctg
     6301 actcacatcc attctacact ggacgtcaaa aattcacaca agcagacgga cgtgtggacc
     6361 gtttcaacaa aaaatacggt ctcaaagacg caaacgctgc ggaataataa aaacgctgat
     6421 aactcaacgt ttagcagact atctgatagg gatagtctgt ttttttatgt tttttgccaa
     6481 tttaaaactt cgtttgatgc tgaaaaagtc gtaggatcat aatgcacaaa acaatgaaaa
     6541 actagtatat agttttaatt atccacttga taaaaacacc ttatattaac gaaatgaaga
     6601 ggggttattt cgaagtaaaa aaccatttac ctaaaattag aagctaagct ttcctttcaa
     6661 ggaaggcttt ttgttttgtt atagacttgc gattgtttgt gagctagatc gttcatttta
     6721 atgacttata cacgcggatg atgggtcttt ttcctctgtt cattcataat attgaacggt
     6781 acagctagcg atgctatact gacctcagtt ccaagaaaac gttaacattt tttgtggggg
     6841 agaggttttt tatgaggttt gatcaattga tcgcagaaga actgattgtt ttcgatccat
     6901 cgatagattc aaaaggatca ttatttgagc gaatagggga tgtgttgaaa aaaacaaata
     6961 aggtaagaaa tgttcaaaaa gtcgttaagg cactattcaa acgagaggag gagatctcca
     7021 caggaataga agatggattt gggattcccc atgcaaaaag caaatatgtg acaaaaccag
     7081 cactgtgttt ttttcataca gccaaattaa cggactatag cgggatagat gaaatgccaa
     7141 ttgaatatat ttttgccatc gtagttcctg aaaaatcagc agatatgcat ctagagattc
     7201 tcagcacact ctccagacga ttgatgaatg cagattttag aaagggaata aaagaagcca
     7261 ctacgccagc agatatcatc agacagatta gtcaagaatg aaaatagtag agtctttgtc
     7321 cgagtatcga tagtgaagaa aaaggaggaa aataatcatg aaaatcgttg gtgtaactgc
     7381 ttgcccgact ggaattgctc atacttatat gtcggcagaa aaattagaag taacagcaag
     7441 aaatctaaac catcaagcaa aattcgaaac acaaggtgta aaaacagaaa atcagttatc
     7501 tgaacaagaa atcaaagagg cagatgcaat cattcttgca gtagataaag aaatcgaatt
     7561 ggaccgattt gccgggaaaa aagtaaagcg tgtctctaca agccgagcaa tcaaagaacc
     7621 gcaagtagta atagacgaag cattgagaga tattggcgta ttcgttgtgt ctaatgaaaa
     7681 agaaccagct gcaaatgaga aaaagccgac aatctataat catttcatga atggtgtgaa
     7741 ttacatgctt ccatttgtga tcgcaggcgg aatcatcatc gcactgagtt ttgcctttgg
     7801 gattacagcg gctgatccca actcagcaga ctataatgtg ttagcagctg cattttcaag
     7861 gatcggagga gatacagcgt ttgccatgat ggttcctgcg ttaggcgcgg ggattgctac
     7921 atcgattgct ggaaaagcag gttttgcccc aggaattgtt gccggattac tggcctctac
     7981 aggtggttca ggttttcttg gcggaatgat tggtggcgtg ctagcagggt atatctgtga
     8041 ttttctggca aataaaatta gcgtaaaaaa agaattttct gctatttatc agctgattgt
     8101 cgtccccttt ttatcgatct taattattgg gcttttgatg gtatttgtga ttgaacaacc
     8161 gatcacatgg gtgttggatg ctttgacaaa ttggctgaat agcttgggaa atacatccgg
     8221 gctattgttc ggattactta tcgggatcat gatggcagct gatatgggtg ggccgatcaa
     8281 taaatcgact tcgactttct cgatcggttt gatgtcagct ggagtaaatg caccgattgc
     8341 tgcttgtatg gctgcaggaa tgacacctcc cctaggtatt gctttagcca ctttattatt
     8401 caaacataaa tttaccaaag aagaacggac ttcaggtcaa tcatgctgga ttttaggcgc
     8461 ttcgtatatt acagaaggtg ctataccgtt tgcggtatct gacccgatcc gcatgatacc
     8521 tagtttgatg acaggttcag cagtcgctgc agggatttcc atggcagtag gagttacttc
     8581 aatggcacct catggaggga tttgggtgat gctaattcca aatgttatga acaatttacc
     8641 tatgtatgtc ttggcaattg catcaggtac agtcactaca gcctttatgc ttggactatt
     8701 gaaaaaaaca gtgaaagaga aaaaacaaga caagtggacg aaaaatgaaa atctaacaaa
     8761 tgaggtggaa gcgtaatgtt atatacaatg aaagagttat tgaaagtggc taaagagaat
     8821 caatttgcag taccagcctt caatatttgc agctatgata tgatgaaagc aatcatcgaa
     8881 gaagcagaga gattagaagc accagtaatc ttagaaattc atcctgatga aatcgcctat
     8941 ttaagtgatg agtttgttgc ggcagtaaga gcctatgctt atacaagtaa actgccgatc
     9001 gttatccact tggatcatgg cggtacgata gaagatgtct tacgggcgat tcgaaacggc
     9061 tattcttccg tgatgatcga tgcgtctttg aaatcattcg acgacaatgt acagattacg
     9121 aaaaaagtgg ttgcgttagc tcatgcggta aatgtctcag tggaggctga gcttggaacg
     9181 attgggaata atggttctgc tgaaggaggt gctgaaacaa tcatttatac agatcctgat
     9241 caagcgaaga tttttgtgaa agaaacagga atcgatacac ttgctgtagc aattggaaca
     9301 gcgcatggct tgtatccgaa aaacagaact ccaaaattaa atatcgactt actgaaagaa
     9361 ttgaaccaac aaatcgatct tccatttgtt ttacatggcg gttcaggtaa tccggacgca
     9421 gaagtcagcg aagcagtaaa atacggagtt gccaaagtga atttgagctc agatcttaaa
     9481 agcgtctttt tcaatgcttt gagagaaata ttgagtgaaa atccatcgat gtacgagccg
     9541 aatgcaattt atccagcagc taataaaaag gtacaagaag tggtcaaaca taaaatggaa
     9601 attttgaaca cagtaggcaa ggctagttta tactaaaaat aaaaatgtgt ggtcaagagg
     9661 gtatgacaat agtcttgtca taccctctta accacacacg atgttcagaa gtagtttttt
     9721 cgaaaaattt cttcttattt ctcgaagcca atcacttctg tcacaacctc ttttttcctg
     9781 ttactatata aagtaaatgg ggtgatttta tgttgaatga agtggagaat agacagcagg
     9841 agattgtgaa ttatctaaaa aacaaccaat ttgcaagagt caccgatttg atcgagttag
     9901 taaattatag cgaagccacg attaaacgtg accttgtttt tctggaaaaa agtggattga
     9961 tccgtagagt tcgcggtggc gccatgctta ttgacaatcg aaaaattgac gtgccttacc
    10021 tgatgaagat caccaaatta gaagaagaaa cagaaaaacg taagattgca gatacagctt
    10081 cttctttgat tcgagatgat atggtcttat tcttggattc cagtacaacc tctttacact
    10141 tagtcagaaa cctagcaaga tttgatggtt tgcagatcat tacgaatggc atcgtcacag
    10201 ctgctatgtt gagtgaatat acaacggcaa aagtatctgt attgggaggg gcaattgttc
    10261 cgaaacgagc aacaatcaac ggttcaaaag cctataacga tgcattgtca tattatgcgg
    10321 atttagcgat aatgagttgt cgcgggctag atttcagcca aggagtaaca gaaactcatg
    10381 aaggagaggc aatggtcaaa aaagctttta gaaaacaatc agaccggctt gccgttttag
    10441 ttacacagca aaaaattgca cataaattca tgcatcaagc cattgctttt catgaaatcg
    10501 attatctggt gacaagtaaa gaactgacaa aagaacagta tgcgaaagta aaggaacacc
    10561 atatcgaatg tctgtacgga taaattatta cgtgaatgtt ctaagtggct tcctcatttt
    10621 tagataggac cttatctttg ctcgcttctc tcagccgatg gtataataat gaaaaggtta
    10681 tcttgttaga taatcttact tttttatcca ggagaaagga gtgcagaaaa tgaaacatgt
    10741 gaaggcgttg gtagtgaagg caatcatgat ctgggcgatt ctgtgggtcg tgctgacagg
    10801 tttatacggt gttagcttta tggattcaac gattgttggg gtaattattg tagtcatgat
    10861 ctatgtgcta ggggatctgt tgatcttacg taaagtaggg aacatcgcag cgacgatcgc
    10921 agatgctggt tcagctgcag ttatcttgtg gctctatcta tattttatga atgatacagt
    10981 cgatatttgg atgaaagtat tgattcctgc tctattgatc ggtgtggcag aatggttctt
    11041 ccacaagtgg ttgctaagtc aaggcatcgt accagatgaa cggaaaatgg aataaatcaa
    11101 cagttaaact attcttatcg ataaatgaaa gctttaaaaa taaatagatg tttaattaag
    11161 aataatcata aactattttt actcaaaaaa ccgctcagat tattttcaga tctgaacggt
    11221 tttttttatc tattcatcaa gatcaaattt catttttcaa tgattcaatc aattcgccct
    11281 ctgcttcatg actatgtttt cggccattaa caagatagag tgctgtacat atcaatggaa
    11341 tgactccggc aatcacatac aatcctctaa atgaagtaag agattttagc atccccatca
    11401 aataaggacc aacgcctaag cctaggtcta aaccaataaa gtaggtagat aaagcaatac
    11461 tgattcgatg ttcgcggacg atttttaaac aaacagcctg tccatttgac ataaacgtac
    11521 cataacctag gccgactaat gctccagaga taagcaatgt cacactattc gtcgttacac
    11581 tcagtaaaaa taatcctgct gttaagaata aatagctagg atacatcacg tattgttctc
    11641 ctttgacatc gaaaatcctt ccagtcaatg gacgtgtaaa tgtgatgacc aaagcatata
    11701 cgacgaagaa aaatgaccca gcagcaacta agtggattac ttttgtataa gaagataaaa
    11761 atgataaaac acttgaataa gaaagaccca tcaaaaaggc aattcctgta atgaaaagtg
    11821 ctttcttttc aataaaacta ttgatattcc atgagcgaag agatattttt tgttcttcag
    11881 ataatggaat attttttaca ggaaagacaa ggcaggcaat cgtaacaaag aagaccagta
    11941 caattgaaaa ccagataatg aaacgaaaat cagtcatatt cagcaaaatc attcctaaaa
    12001 atggcccaac agcggcagct aaactagtac ttaatccata ataattgatg ccttcccctt
    12061 ttttagatgc agggatatat gcagtaacga tagcatttgt agctgtagaa actgtaccgt
    12121 agccgaatcc attcaagaat cgaactaaat acaatgcccc gatagttggc gtataaagat
    12181 atgctaatgt tgaaaggaga tagaagatcg cgccgcctct taaggtaaac ttccgaccaa
    12241 atagttctaa ctgtttcccc atgaataagc gtgccagcaa tgttccgatg atatagatcc
    12301 cagaagcaaa acctgcttgg ctcaagctgg cgtgtaaggt gtcttgcgca atgactgcca
    12361 tgatgaccat caacagataa taaatcaggt agacaataaa attgatcaat gtgattccaa
    12421 taaaatattt attaaataat ttcttttcca tgtatgttat acattcccct taagctgaat
    12481 atgcttttaa tttttgtatt tgaacttcta catcgtgata attggatgga tagcgatcaa
    12541 caagttggcg aataaataaa tctcttgtag acataaacag atttaaaaat tcttcgcatt
    12601 cgtttgttgg attctcatcc actgctttga tcaattcgat cgagttttga tacgcatcat
    12661 ttaaaaagac taaagaagct ttcatatcaa atcttgtttg atatgctgtt tctaatcgat
    12721 taatgattgt gttcatccag taattcatgt tttttccctc tattcttaaa aaattttact
    12781 tctgcagtat aacgaataaa aaaaaggaaa aaaatcgcat aagtgatgga aaaaggtact
    12841 taacaaatgt taatatcatt agtaaaatac ctttggactt taaaaagttt taaagtactg
    12901 tatgatcttt ttagatgaaa gtttggatgg gggagcatca ttggatagta gagtcttaaa
    12961 ggaatatttg gataaaaatg attttccgat tatacgcaaa ggatatcata aatatttgac
    13021 attcgaaggg atggaagatt cccatacgta tatcttgaaa gagggaacgg taaaagcaag
    13081 tgttatttca aatgatggga gagaatttaa ccttcgctat atcaaagatc ttgaaatcgt
    13141 ctctctttta aaagacgaat attcacaatt catcgattcg ccgtataata ttcgagttga
    13201 atcagaatat gcagagtttt atcgaatcaa tcgtgtgcgc ttttggcaaa atatcaacca
    13261 agacccaaag ctacaacact atgtaaaaga gtattaccgc tatcgtctgc tttattcgat
    13321 gaaaaaaatg cagcaaatgc tattaaacgg aaagctggga gctgtttgta cgcaacttta
    13381 cgaattatat gatttattcg gtgtgcggac agaagaaggg atgctgatcg atttttcgat
    13441 cacgaatgaa gaaatcgcca aattttgtgg catctcaact gctagcagtg taaacagaat
    13501 tttaaaacaa ctgaaaaaag atggtattat caccgttaaa gaccatcata tcgttatttt
    13561 gaatatggac ttgttagaag acaatattat cttttgatga acaaagaggt tgtgaaatca
    13621 gcgttttgac ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    13681 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct
    13741 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    13801 tattgtgact tgtgaccgag gagttggtag gagaacaccg tttaccaaga ggttgtgaaa
    13861 aagctgttct gcatcaagta ataagaagag ccaagtaaaa acagtctctt atgtttttaa
    13921 cttggctgga cttatcaccg tagatgtaag cttttgaacg ccgtttattt gcaacaaaag
    13981 gggctgtgac acgatttttg tcacagccct ttttttgcga acattcattt agcaattttt
    14041 agaaacttct gccgccaccg ccaaaagtcc cgccaccgct agaatgggta gtacttccgc
    14101 caccgccgct tccaggaggt gggcttttgg ggattcttct tgtcgtgaca aaagaatttg
    14161 tcagacgatc ggttttttca gttaatttga tactggattt ttctcggaat ggatatttgt
    14221 agatgcctga tttcaattga taacgcgaaa cagtcacaaa ataaaagaca agactaagga
    14281 taagagccaa aacaacagca atgatgattt ctactgtcgt caaaacctta tagcgtgtaa
    14341 ttttgccagt ttcttcgtct acacgataat gtcctcctgg aactccgtct tccacatatg
    14401 ttgctgattt agacaggtaa gcttttgcag cattgtaata ggcagcattt gtcatctgat
    14461 cataaacatc atctaagatc gaatcgattc tgctatcagt caaataatcg atcatattac
    14521 cagaagtcga aatgtagatt tcacgctgtc ccatatctaa tagcaatact gagccatttt
    14581 gatcatttcc gactgcgtct cttaagtaat tatcagcaaa gtctcttggt tcttctgaat
    14641 tagatgtagt cgttacgatc aagactcgtc ctttgatttt ttcattcagc ttttctgcct
    14701 gttgttccaa agattggatt tgatctgttg tgaataaccc agcctcatca tttaccgtag
    14761 ctgtatctgc atgagtagcc accggccagc caacacttac tagcaaaaga aagaataaaa
    14821 aaaatttttt catcatatca agtaacctcc gatcagaaag aggatcgcta gcccagcgaa
    14881 aatcccaaga gcggtcagtc ccaatttttt ctggctgatc ggcaggacac cgctgacctt
    14941 tcctgtttgt ccattcattg cgtagtagta gactttttta cttgagtcat tgcttcgata
    15001 agtaacaagc cataccggca gtaatacgta attatttact tcgtttgtga ttgaagcaga
    15061 agttcgcaca ccggtcaatg ttgtatatcc atttgcggta tcttttaaca gcttctctga
    15121 atagtctttt aattcagatt gaatctgtga tttgattgcc tcatattcga tatctctttt
    15181 ttctgcttgg aacccggcta aatactggct tttaaaatca attgctttat ctaacgggaa
    15241 aggttgcacg ccttcgacca tcttttgttg cgtatttttt gataaggcat tttttactag
    15301 ttctttaaaa gaaagcttgc ctgctcttga aacagcaaat tgcttcgttt ctgtgtattc
    15361 gatgtcacct atccgccata tgcggataac tgtaccattc gcctgcatgg caccatctac
    15421 ttcagcatct gtagcccaat aagggaaata cacacctgtc aacttatcga tttgatcctt
    15481 attgaaaaaa gctttgggaa caaaccattt tttcttggtc caagcgagaa atctgttgat
    15541 tgcttcttct ttctcgacag caaaaggaag tactttattt ggcaagaatt ctccgcttaa
    15601 acgtcccgat agtacgacgg gattatggca gtagtaacaa taagtcgcag cagttgtcgc
    15661 atcagtcacg atctctgcac cacagttggg acaaagaaaa agatctatcg ctgcggtatt
    15721 atctgcagat tcgtccgctg tatctgaaaa accgcctgcc tcgtcaaccg ctttgcgctc
    15781 ggcttcattc atatcggcaa gctgttcttg ggctgtaaaa gtaaaatctt cggtaggtga
    15841 aggttcttct ttgctttctt cgccagccat acgcgcttct ttttgctttt gctcatattg
    15901 gctgacttct tcttctgtat agatattcag gcagtattcg caatgaaatt tttggtcttt
    15961 gggatcaaaa gtcaacggac cgccgcaatt aggacattta tgtgtaagaa catccatcag
    16021 ctgcacacct ttctaaataa attaattaac ggggattcct ttaaacggtt taccacagtt
    16081 tggacagaat ttagggatgc cgttggaaag atccacgatc tcattgcatt cactgcatcg
    16141 catttgtagc ttaggctgaa cgttttctac aggtttggca gctccgcagt tggaacagaa
    16201 ttttccggaa ttttctgtcc cgcaaactgg acatgtccaa gtatccgcat ttccttgtgc
    16261 agtttgttga ttttgttttt ctgcttgttg tttgatttgt tcttggttat tttgagctgc
    16321 ttgagaaaga taagaaccat ttgcattcat tcccatacct acacccataa agccggtcat
    16381 tgctcctgct tcattttttc cggctgcttc cataccacgt gcaatggaac cttgcacgta
    16441 tccttcacgt acgctaggat cacctagcat cgccccttcg ttacgcatat tgatcagttt
    16501 cacagaatca tcggtgtaag aaatgctggc tacagccaca gaaacaatct ccatcccacg
    16561 taattcacgc caagaatcat ccaatgcagt atccatgtat ttgcttaatt ccaaactctt
    16621 agatggtaca taagaaatcc gttgaccatc tgcagacatc tgattgattg ctgattgtag
    16681 cgctgttagg aattcagcta aatactgctc attgatatca ttgatttcca cttgtgtttt
    16741 gtttttaggg attgcgtttg tataaaataa aatcgggtca acgacatgaa tagaataagt
    16801 gccatgtgcc cgtaagaata attctgcgtt atagaaattg tcaaagtagt tcaacggcgc
    16861 gcttgtaccg aatttgattc cctttatttc ttggagattg atataaaaaa cttgttgttt
    16921 ctgcggtgtg acgccgccaa atttgaatcg gtcaaaagtt tcagcaattg cttctttcag
    16981 tgttccattg aacatggaag gagctgcgtc atttttaatg gtgtaataac cttcctcagc
    17041 ggtataatcg atgatttttc caccatcgac caaaagcatc atcatgtttg gatagacatg
    17101 gacgactgtt ccgtctgtta aaacatcttc tgttccttta cgattggagc cacgtttgtc
    17161 atctttgcgg actttgacac cttttgtcat gaccgttgta tcactcatat tgtctggttc
    17221 gatgacttct agccattgat ctgccagacc gccgccaacc atacttgttg ctgctttgat
    17281 aagacccata aaaaattcct cctctgattc agctagtgcc aaacaattat tgtttattta
    17341 ttataccata ggaagatgtg aaacttctcg gactagagac ggaattgaaa tggaaaattg
    17401 cgtattgttt aaatacctga aaaagccgag aaggatagta gtttttttac ctaatatcac
    17461 gtataataag agatagtgaa ttttttaaaa aagggggatc cttatggcat atcaagcact
    17521 ttatcgagtc tggcgttcgc agcgtttcga agacatcgtt ggacaaaaag ctgtgacaca
    17581 gacactaaaa aatgctattg tttcccacaa aacctcacat gcctacttgt ttacaggacc
    17641 cagaggaacc ggaaagacga gtgcagcaaa gattttcgca aaagcaatca actgtccgaa
    17701 cagtaaagac ggtgagccat gcaacgaatg cgaaatgtgt cgatcaatta ctgctggtac
    17761 gcaagaagat gtgatcgaaa tcgatgctgc cagcaataac ggagtcgaag aaatccgatt
    17821 tatccgtgat cgggcaaatt atgcgcctac aaaggcagcg tacaaagtgt acattattga
    17881 tgaggtacat atgctttcaa caggagcttt caatgccttg ttgaaaacat tggaagagcc
    17941 aaaagaaagt gtcatattca tcttagcaac gaccgaacct cacaaaatac cagctacgat
    18001 catttcacgg actcagcgat ttgattttaa acggatcaat acggcagata ttgtcgaaca
    18061 tcttgaattt atcttgaaag aatcgactat cagctatgaa ccagctgcat tgaatgtgat
    18121 tgcccgttct gcagaaggcg ggatgcggga tgcattgagt attcttgatc aggcaatctc
    18181 gtttagtgaa ggaacaatta ctttagatga tgccatgcaa gtgacaggta gcttaactga
    18241 cgaaatgatg gacagttact tgagtgcttg cgtgaaaaaa gaagtaccag aagcattgga
    18301 aacattgaac caaattctcg cagccggcaa agaatcacgt cgctttctag aggatatgtt
    18361 acaatactgc cgtgacttgc tgatgtatca gcaagcacct aacctagttg aggaacaatc
    18421 gagtacgatc agaagtacat ttacagaatt ggcacaaact gtttcagcgg atcagctgtt
    18481 ttcaatgata aagattttga gcgatactca aaatgaaatt cgttttacca atagtgccgc
    18541 tatttatttg gaagtcgcta cagtcaaact tgcacagcta ccgtctgctt ctgttccttc
    18601 agcagatgta gcatttgcag atgatcagat agtaaaacag ctacaacaag agattaagga
    18661 actaagaaac gaaatcaagg aactacgcag taatacagga attgagaata caaaagtaca
    18721 gccggtacag caaacaaatc ataaaaaaca gacaagtacc tatcgtgtac cgacggaacg
    18781 agtatataaa gttctgcggg aagcgacaag acagcatctg aaccaagtaa aagaagtatg
    18841 ggaagacttg ctgatgagtc tatctgtcac tcagcgagca atgcttaaag caagcgaacc
    18901 agttgcagct ggacctacag gactagtgat tgcgtttgat tatgagattg tttgtcaaag
    18961 agcagcaaat gacgaagaat tgcagctttc ggtacacaac aacttaagcc gaatgatcaa
    19021 agattatgcg cctgatcctg tttatattac aagagaaagc tggccagaac ttcgaagaaa
    19081 ttatttgacg caggcacaag aagagggaac agaaaactct tttgcttctg aagactcaga
    19141 aggagaaatc gagttgcttc ctcaagaaca agaagtacca gaagaaacac ttgtagtaaa
    19201 agcagaggaa ctgtttggct ccgaagctgt tacagtggta gatgattaat aaaaaggagt
    19261 agataattat gatgcgtgga atgggcaata tgcaaggcat gatgaaacaa gtacaaaaaa
    19321 tgcaaaaaga aatggcagaa gcacaagagc tgctaaataa aacagagttt acaggtaaag
    19381 ccaccaatga attggtcaaa gcggttttta caggcgatcg tcgcatgaaa gatatccaaa
    19441 tcaatgaaga agtcgttgat cctgatgata tcgagatgct tcaggatctt gtcgtaatgg
    19501 cagtcaatga tgcgatcgca caaattgatg tggaaactga gaaaaaatta gggaaatacg
    19561 caaaaggttt gccaggattt tagttttaaa ctgaaggagt aatgaatatg caatatccag
    19621 aaccaattgc gaaactgatc gacagctaca tgaaacttcc aggaatcggt cagaaaacag
    19681 cgacaagact cgccttttat acgatcgata tgaaagatga agtagtaaat gaatttgcta
    19741 aatctttgtt aagtgtaaaa cgcgatttgc atttttgtag tatctgcgga aacatcacag
    19801 aagaagatcc ctgcgaaata tgtagaaatc cttcacgtga tcgaagcgtt atcctagtgg
    19861 tcgaagagcc gaaagatgta atggcaatgg aaaaaatgcg agaatatcac ggactttatc
    19921 atgttctcca tggtgtgtta tcaccgatgg aagggacagg tccagaagac atcaatattt
    19981 cttctctgtt gcagcggctg catgatgcag atgtaaaaga agtgattatc gcaacaaatg
    20041 caacaacaga gggagaagca acagctatgt atctctcacg tctgatcaaa cccgcaggta
    20101 tcaaagtgac tcgtctagca catggtctgt ctgtaggctc tgatatcgag tatgcagatg
    20161 aagttacttt attgaaagct gtcgaaggaa gacgtgagtt ataaatttct aattaagtgt
    20221 tttttcagat acaatataaa agcagcacaa gagatggggg caacaatgtg aacggactat
    20281 ttattacaat cgaggggcct gatggtgcag gaaaaaccag catcttgaat gaactttacc
    20341 ctcttttgaa agaagtagca aaaacagaaa tcattcaaac aagagaacca ggaggcattc
    20401 cgattgctga agagatccga gcagtgatcc ttgatccaaa aaatgatcgg atggatgaac
    20461 gtacggaagc actgctttat gcagcagcaa gaagacaaca tctagtcgaa aaggttctgc
    20521 ctgctctggc aaaagggaaa atcgttttat gcgatagatt tgtcgacagt tctcttgctt
    20581 atcaaggtgc aggccgacgc atcggcgtta ccgagattgc tcggttgaat gagtttgcta
    20641 cagaaggtac tacgccggat tttacccttt atcttgacgt agattcagac accggattaa
    20701 gaagaatcaa gaaaaacaga caaaatcaaa tcgaccgttt agattcggaa ggtttggaat
    20761 tccaccagcg tgtacgtcat gcttacttaa agctggcaga agagaatccg gaaagaatcc
    20821 acaaagttga tgcaagaaaa agctttgaag aagtgctgca aacaagttat catacaatta
    20881 ttgaacagta tccgcaattt tttgaaaact aggaaggtga acgtttatga aaattatttt
    20941 agcgattatc caagacaaag acagcaaccg tttatcaaat gaattgattg atgcaaatat
    21001 ccgtgccaca aaactatcat cgacaggcgg cttccttaag gctggaaaca gtacgtttat
    21061 cgtgggtatt gaagatgacc gtgtagaaga agcattggaa atcatcaaaa aaacgtgtga
    21121 atcaagaaag caatttgtat ctacacctgt tacactggat atttcgatgg atgggggcgt
    21181 cccttatcca gtagaagtag aagtaggcgg tgccacagtc ttcgtcttac ctgttgaagg
    21241 attccatcaa ttttaggagg agctgagcta tgaatcaaga aataaacttg gctcaaatgc
    21301 aaccgatcgt gtatcagcag cttcaacgga gttttgagca tgggcgtatc gctcatgctt
    21361 atctttttga aggggagaaa ggaacaggaa aacatgaagt aggtatatgg cttgcacagc
    21421 acctgttctg tacccaaatg aaagacaatc tgccttgtgg aaaatgcaac aattgtcagc
    21481 ggatcgaaaa gaaagaacat ccagatgttt tggttattga accggaagga caaaccatca
    21541 aagtcgatca gatcagacgt ttgcagacgg aattcagtcg cagcggttac gaatcaagaa
    21601 aaaaagtttt tttgatcaaa gaagctgaaa aaatgaatag cagtgcggca aacagtttgt
    21661 tgaaattctt ggaagagccc cctggagatt ttttagcggt gttagaaaca gatgcagtag
    21721 gaagaattct ccctactatt caatctcgtt gtcaagttct gcattttcaa gaactttcga
    21781 aagaagcact gatccatcga ttgcaagaag agcagattcc attggaaaaa gcaaaacttt
    21841 tggctttctt aacaaacagt ttaggaaaag cagttgaaat ctctcaagat gaatggttta
    21901 atgatgctaa ggacttgatt tatcaatggt tcgtttactt gcaaaagcaa gatactcaag
    21961 cctttatcta tgtccaaaaa aaattggtaa aatcatttaa agaaaaaaca caacagttta
    22021 caggattatc tgtcttgatg ttttattatc aggaagcaca gcgtcaagca gtagcaaacg
    22081 gcgaatatgg caaagtgaaa cagataaacc agataattga acggatattg atcgcagagc
    22141 agaaactacg cagcaatgtc agttttcagg ctgttgcgga acagttcgtt ttgcatacga
    22201 ttttttagct tgccgcaaag gatgatgatc aaggaggatg gaaatggtag aagtagtagg
    22261 tgtccgatat agagatgcag gtcatatcta ttattttgca ccaggagact ctgaatatgt
    22321 ttatagtgac aaggtattag tcgaatctca gcaatcctta catattgcta ctgttgtcct
    22381 tcctaaaaaa gagattgatc ccaatgacct tcctgaagct atccgtccga tcttgcataa
    22441 agcgtcacaa gctgaattag aaaaagacaa aaaaaacaaa aaagatgctt atgatgcatt
    22501 gaaaatcgca aaagaaaaag tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga
    22561 atacacattt gatcgaaata aattgatttt ttctttcaca gcggacgggc gaattgactt
    22621 tagagaattg gtaaaagatc tggccgctgt atttcgtact cgtattgagc ttcgacaaat
    22681 tggtgtccgc gatgaagcaa agataatagg cggaattggt ccatgtgggc gtccattatg
    22741 ctgttcttct tttttaggcg atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag
    22801 tttatctttg aatccaacaa aaatttccgg tttatgcgga cgcttgatgt gctgtctgca
    22861 atatgagagc gaagaatacg agacagcgaa aaaagaactc cctgactttg gaaaagtaat
    22921 tgctacacct gatggtaaag ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag
    22981 tattcggctg actggacggg aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt
    23041 tatccaagta ggtgagatga atggataaga gatcattata cgacggattg aatcaattag
    23101 aatcagagtt gagaaataca ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga
    23161 tcgtagaaaa aaatacaact ttggagatgg aaaatcaacg tctgcgggaa cacctgcggg
    23221 aattgaacca agttgcacaa gctccagctg aaaatgtgaa gcaggaactt tctaagtctc
    23281 gtatgaatct tgaaaaaatt tatgaagaag gattccatgt ctgctacgat ttatatggat
    23341 caagaaggga caatgacgag ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg
    23401 gtcgatgacc aataacaaga ggagactgga gcggagaaat actgctccag tctttttatt
    23461 aaggagattg tgacagatgt ataatcaaaa aagtttcaag ggaaatccgg tatatgggga
    23521 tttatatctt gtaccgacgc caattggaaa tttagaagat atgagctaca gaagtgttcg
    23581 tatgctgcaa gaatccgatc ttatcgcaag tgaggatacg cgtaatacac aaaagttatt
    23641 gaatcatttt gaaatccata caccccaaaa aagtttacat gaacataatt ataaagaacg
    23701 tgtccctcaa ttgattgctt tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc
    23761 aggaatgcct tccatcagcg atcctggaca tgagctagtt ttagcctgca taaaagaagg
    23821 gatttctgtg atctctatcc caggacctac agctgggatg acggctttga ttgcttcagg
    23881 gctagtgcct caaccatttt tattttatgg cttccttcca cgtaagaaaa aagaacagaa
    23941 agaaacattg aaaaagctaa aagaacagac agttacgctg attttttatg agtcacctta
    24001 tcggatcact gctacattga cgaatttctt agaaattttt ggaaatcgcc aagtcgtttt
    24061 gtgtcgggaa ttgacaaagg tccatgaaga ataccttaga ggaagtacgg aagagctact
    24121 cgcttatatc gaagaacatc cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt
    24181 gttcgaaatg ccagtgacgg aagttgatga agagttggga acgttaaagg aacaagtaga
    24241 agcaaagatc gcttcaggcg aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa
    24301 tgggctgaaa aaacaagaag tctaccgtca gtaccatgaa ttagactgag atagtataag
    24361 ttgtctataa tgaaagacta atgaggaaat cagctactaa cttttcaaat ctcttgaacg
    24421 cgaacgaatg ttcttgtata atgaataaga ggtgaggatg atggagctgc aatttccttt
    24481 acataatgat acatcaagaa aaatcattca tattgatatg gatgcttttt tcgcttcggt
    24541 tgaagaacga gatcatcctg agtttcgcgg acatccatta gtaattgcca gacaccccag
    24601 tgacactggt gggcgtggag tagtgactac agcaaattat gaagcaagaa aattcggaat
    24661 tcattcagca atgagtgcac aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc
    24721 tggcgaccat cataaataca gcgatatttc caaacaagta cgggaagtat tctatcgtta
    24781 tacagatatt gtggaaccag ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa
    24841 aatcaattgc aagtcagcca tcaaaatcgc ccgaatgatc caagcagata tttgggagag
    24901 tgtccagcta acatgttctg cgggagtaag ttacaataag tttttggcca aattagcatc
    24961 agactatcaa aaacctagag gaatcacggt agttgcccct caagacgctg tgccattttt
    25021 aaaagctttg ccaattgaaa aatttcatgg ggtagggaag aaaactgtac ctagaatgca
    25081 tgagctaggg atttatacag gaaaagacct atatgaatgc acagaaatga tgttgattcg
    25141 aaattttgga aaaatgggct attctcttta tcgtaaagtc agaggaatcc atgattctcc
    25201 agtaaacgtt acaagagagc gaaaatctgt gggaaaagag catacttatg ggcagccgtt
    25261 gcaaacggaa gaagcagtgc tgactcagtt gcgccaacta gctgaaaaag tagaagaagc
    25321 actgagaaga gtccaaaaac atgggaaaac agtagttttg aaggtcaggt acacggacta
    25381 ttctactgtg accaaaagag tgacattgcc agaatatatc tataaaaaag aagcactctt
    25441 ttatcaagca agccttatat gggaggaaat cttgggagta gaaaaaggaa tccgattgtt
    25501 agggattact ttgacgaatc tggacccgat gacttatgaa aacatcgtcc ttccattatg
    25561 ggaaaatcaa gaaatataaa aacagctttg agagttgaaa ccggaaatcc gttttctctc
    25621 ccaaagctgt ttttatatca agtcttgctt tttgatttgt tcataggttt ctgttgcatc
    25681 cgtacatttt tcccaaatca caacttgatt ggattctaaa gatttttgga cgtcaatgat
    25741 ccgctggttg ctacttccgc gaaattgtag ttttaggtca cgtttgctta attcaaagcg
    25801 tccgtcaaca aggatatcga tttgcgaaag gagttctaat ttatcttctg aatcttgtag
    25861 caattcatca aaggtatagc ctgtccatga ccagatatct tttgtcgtac cgaactcttt
    25921 tcggatacgt cgtacgactt gcagacaaac atctgtattc aaaaaaggtt cgccaccaag
    25981 taaagttaat ccttgcacgt aatcgttgcg caagtcagcg atgatttgtt cttctaattc
    26041 aggtgtgaat ggtttgccat atcggaaatt ttgtgcagct ttgttgaagc agccttcgca
    26101 ggcaaataag cagccactaa cgtaaagact gttgcggaca ccttcaccat cgacaaaatt
    26161 aaacggtttg tagtcagcaa tatagttttg gctgtagtct tttgctaacc attcttttgg
    26221 ttttggattt ctcataaaaa aacttctttc atttaaggtt caaagatata gctattgaag
    26281 ataaaatcta ctcgaccaca taaataagaa caaactgacc taaatccatt gttcaagaaa
    26341 gcttgttctt attttgatag agggcgttca aagatacaaa gccatcgaaa gagggtggtg
    26401 aaaaaagact tgtcatagcc tcttattctg aataaacggt gttcaaaagc ttgcatctgc
    26461 agtaataagc ccaactaaac gaaaaatatg aggaactatt tttgtttagc tattcttatt
    26521 acttgatgca ggacagcttt ttcacaacct catttcatgt gtttcacacg tgaggaaatt
    26581 tctttatggc ggccatgtac catcggacga gcttgcggat ttcctaaata gccacaagta
    26641 cgtttgacta catcacatgt ttttggatca tgatttccac attctggaca ttcgaaccct
    26701 cgttctgtcg gtttgaaatc accttcgaaa ccacattgat agcaatgatc gattggcgta
    26761 ttcgtaccaa gatacccgat tttgtcatat gcaaaatccc atacagcttc taaagctttc
    26821 ggattttgtt ggagcatagg gtattcacag tagtggatga atccacctga acaatatttt
    26881 ggataatcct tctcaaattc caatttttca aatggcgttg ggtttttacg cacatcatag
    26941 tggaaactgt ttgtataata ttctttatct gtgatatttt cgatgatgcc gaatttttct
    27001 gtatccaaac ggcagaaacg gtcagttaga ctttcacttg gagttgaata gacactgaaa
    27061 tgataaccat attcatcccc ccatgcatct gcattagctt ttaattcttt gacgatatca
    27121 agtgtgaaag cttttgcttc tgggtttgtt tcccattcgc caccgaagaa agctgaagca
    27181 acttcataca atccaatgta gcctaacgat acagtagctc gacggttttt gaataattca
    27241 ttgacagaat cggtacgtga aagacgtttc ccgaaagcac catacatata taaaattggc
    27301 gcatttgctg gaatggcttc tttacaacgt tctacacgat agagaagcgc atctttcatg
    27361 atttctaagc ggtcagacag taactgccag aatttattct gatctccttt tgattcgatc
    27421 gcgattcttg gcaaattcaa tgtaacaaca cctaaattca ttcgtccgac atttacttct
    27481 tgaccatttt cgtctttcca cccttgcaag aatgaacgac agcccatagg tactttgaaa
    27541 cttccagtta attcaacgat tttatcgtaa ttcagaatat caggatacat tcttttcgtt
    27601 gcacattcca atgctagttg tttgatgtcg taattaggat cttcagggtt taagttcaca
    27661 ccttttttca aagtgaagat cagtttaggg aagatagctg tccgcttttc actccctaat
    27721 ccattgattc gaatctgtaa aattgctttt tgaatttctc tttcaaacca gttttctcct
    27781 agtccaaatc ctaatgaagt gaaaggagtt tgtccatttg aagtaaataa ggtattgatt
    27841 tcatattcca aactttgcat agcatcgaag atatcttttt tggtttttgc ttttgcatat
    27901 tctttttggc gttctggcga atcgatccat tcttccgcgt cttttagatg ttttttatag
    27961 ttcaattctg caaaaggagc aagcaattca tctgttcggt cggcagaaca gccaccatat
    28021 tgacttgacg ctacattggc aataatctgt gagatttgtg ctgtggcggt ctggatagat
    28081 tttggcgatt cgacttcggc attgccgatt ttgaacccat tgttcagcat gcctttgaaa
    28141 tcaatcaagc agcaattcgt catcggtgta taaggatgat aatccaaatc atggtaatgg
    28201 atatcgcctt tttgatgcgc atttgctata tgtggtggca acattttcag tccgattgat
    28261 tttccaacga tcccggctgt cagatctcgc tgtgtattga aaacattact gtctttattt
    28321 gcgttttcgt tcacgacagt ttggtccttg ttgattagct tttcaatcga aaagttgata
    28381 tctgttgctt tactgcgagc aaaatctcgt ttggtacgat aattgatgta ctcttctgct
    28441 aattcatatt cgtttttttc tagtaatata tgttccacga tgttttgaat ctcatagatt
    28501 ttgacatttt cagcaaaccg gcttttgatt tcttggacaa ccaagtctgc aatcatttga
    28561 attttttcat aagtaagtgg tgataatgag ccgtgaattt tcttttctgc tttgatcagt
    28621 gcttcattga tcttttgttc atcgaaatca acatggcggc catcgcgttt gatgactttc
    28681 atctgggatg tctctggctg ctgagtaacg acagtttttt cttcataatt tactttcatt
    28741 atcttcaact cctgctaaat tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa
    28801 ctaaatatcg gctatatata gtattcaatg tgctgtataa ttggatttct atactatatg
    28861 tagtgtatat tttattgtga aaaaaggtga aaacttgtag ctggtggatt tgttttttgc
    28921 atatttaatt ttgctgaata aaataattga ataaaatttt ttattcagtc caagtaaaag
    28981 tgacaaaaac ataagagcaa attattcgta gatatgctac tatgcaaata aatagaaatg
    29041 aaaaatgaaa gaggaggaga agaattggaa aagatgatcg aagtgaaaca tctaagtaaa
    29101 agctacggca gcaaactttc tcccgtcaaa gccttagata atatctcatt cacagtagaa
    29161 gaaggcgagt ttgttgggat tatgggccca agtggtgcag gaaagacaac gctgatgaat
    29221 atcttgtcca cgatcaattt gcctacgatg ggcagtgtct cgatccaagg aaaagaaatc
    29281 acaaaaatga aaaacagtga gctcagtgac tttcgtcgaa aaaaactagg attcattttt
    29341 caagagttca atttgatcga taccctaaat gcaaaagaca atatcctatt gcccctagca
    29401 gttgaacgga tgactaaaga ggaaatggaa aagagagtaa gacatgtagc tcaattgttg
    29461 aatatagaag aactgctcaa acggtatccc gatgaacttt ctgtcggaca gagacaacga
    29521 attgcagctg ctagagcact tgttgttcag ccacaagtga tttttgcaga tgaaccaact
    29581 ggctctttgg actcgaagtc ggctacagaa ttgttgaact acttgaagac catgaatcaa
    29641 aaagaaaaag caacgatact gttagtcacc catgatccat atacagcaag ctactgcgat
    29701 agaattttat ttattaaaga tggtgtgatt ttttctgaag ttgtcagaag agggacaaga
    29761 agagagttct ttgagaaagt aatagacatg caggcgacga ttggcggagg aggaaaaatg
    29821 aatgctgtat aaactagccg ttaaaagttt ttttcggcag tcacgaggct atcttgttta
    29881 ttttttcagt ttgactttat ccgtaatgat ctattattcc ttcagcgcaa tgacctacga
    29941 tcaatcttta gtcagaagag caagtcaaga tacttcgata gatggtggat tgagttttgg
    30001 tggatttatc attactgttg ttttgctttt tttcgtattc agtgccaacc gatttttctt
    30061 gaatagaagg caaaaggaaa taggcatcta tcagcttttt ggtatgaata aactgcagat
    30121 atcaggtata tatgtgttag aaatcatgat aattggattg ttcgcctgta tttctgggat
    30181 attactcggt attatttttt ccaaactctt ttcaatgatt ttggttcgaa tgatggatat
    30241 ggatcttact agtccatttt ttatctccat tccatcagta gtagatactt tatttgcttt
    30301 tttcatcatc cttttagcag tatctgtata ttcaatatgg aaaatatggc gctatccaat
    30361 gattcgaagt tttggagaaa aagaacaagc agatagttca acaatgcgct tcaggacaag
    30421 gcaccgatta ctcgcagtca tcgggttact acttttaacg agcggatatt ttggagctag
    30481 tcattttagg gaaatcactt catggatgat cagttatcat tatcttgata cattagtttt
    30541 cttttttcct tttttaattt ttttcgtttg tgtggtcggt acttatttgt ttttctgtta
    30601 tacatttcgg ttatttttga tcatattgag taaatggaag atttattact atggtatcaa
    30661 ttttttgata atgggtaaca cgcaagttca tttgctcaaa agttggcgta caaattctct
    30721 gattacagtc attctcggaa tttctttaat gacaattggc ggcacggcta gtatctctac
    30781 tattttatcc taccaggaga aagtttcctc cccgatggac tatcagtttg atgttgagac
    30841 agagaaaaaa atcaagccga ttctagcaga ggaagggcag aagataacta aagaaatcac
    30901 cttgcattat aaagtcattg ggagtatcta tagtcaaaat ctcgaagaac ctatgtttca
    30961 aactgctaat ctgatcacgg aaacacaata tcaagccttc agaaaagtta atccagattt
    31021 gccgaacatt tctttgaaat cacctggtca tactgttttg cttgatcaaa tgcaaactct
    31081 ttttcggaat atttcattat atggatctag tattggactg ccagataatc aagaattgaa
    31141 gattcaacaa atgcttccaa gtgtattagg tgacgaaaca atggtatatc taagtcctgt
    31201 actgattgta gatgataccg tttttgaaaa agcaaaaggt gtggattatc aagtagtcaa
    31261 tgtagatgta tcaggtggaa ataatgaaaa aatagatgaa cgactgagca atgacctgtc
    31321 gataaagtgg ctcaatgctg tttattattc ttatgatatc gtaaatggaa gtctacaagg
    31381 agaaattagc acaaagaaac ttccggatga ccaaatgaag gaagcacaat ttagccaaga
    31441 aagctctcgt ttgaatttct cttccagcta ttcgaaattg cgtgcagtta atcggcgaat
    31501 cggtatcaat atttttgtca cactatttgt cgggatgatc gtgattattg ctactggtag
    31561 tattttgaca gtccggcaat tagcagaagc agaggaagaa aaagaaaatt atcacctatt
    31621 gactaagctg gggattccac aaagaaggat cagacggatg atcttcgagc aaaacgcact
    31681 taccttcttt ccacccatga tattagggat cactcacgca gtatttgcaa tcaatgtctt
    31741 cactcagtat ataaaaactg ccgattactg gttggcttat cttgtgagtg gcttattgat
    31801 ccttatctat cttttgcttt atgtcattac gagtcagtta tattgtcgga ttatcgagga
    31861 atgatatacg gaataagaca agataataaa tagaactaaa ttttgaatac catttatttg
    31921 gaataagagg ttgtgacacg atttttgtca caacctcttt attttttata ttttttaaaa
    31981 gtacgagaaa ttataaagaa aaaagcagtg atttgatgtg ctatttttat aaaagctatg
    32041 tgacaaaact gttagtaaaa aaacggtttc tgtaagctag acacatgttt ctttttgtca
    32101 aaattagtta cactttgtgt gaggttatat gaggagggaa tccaatgaac aaatggcgtt
    32161 ctaatccaaa attgaattat tggggaaaat tcatcattcg aacaattttt tatagtgcag
    32221 tactgctgtt cctgatttat ctgtatcact ataaaaatat tcaaggagga acttttatct
    32281 ataacgaatt ttaggaggaa gagtatggaa atcaaaacga ttattgaagc aatcgatgat
    32341 tggggaataa atgaacctga tcgagttgct tatcaggcgg atgagacgca tacttttggt
    32401 gaattgaaac aagcatcaga cgcacttgct tattatttag aaaaaaaggt agaaggcgat
    32461 ggaccaattg tcgtttttgg caatttggag tttgaaatga ttgtctcatt tctaggggtt
    32521 gtcaaatctg gccatgccta tttgccgatt gaagagcata cacccaagga gcgtatccta
    32581 tcgatctttc gggtggctaa accatctatg gtcatcagta tcggggactg gatagaagat
    32641 attccttcgg ttcctgtcat aacaaaaaaa gaatttcaag aaatcaagaa gataccagta
    32701 ggattttatc ccagaaatag cgtaaaaggt acagaaaatt tttatattat ttttacttct
    32761 ggtactactg gagaaccaaa aggcgtacaa atcagtcatg ataatctagt cagctttgtt
    32821 caatggacga tggaagactt tgggatcaag cctggtatgc atttcttagc ccaagcgcca
    32881 ttttcttttg atttatcggt attttctatt tatcctgcat tagtttctgg cggaatgctg
    32941 aagccgttaa ataaagcagt tgttcaagac tttcgtcaat tattcgcgac attgccagcc
    33001 ttgaagttga atgtgtgggt ttctactcca tcatttatgg atatttgctt gatggaacct
    33061 acattcaatg gagaaaatgt gccggaattg gacatgtttt tattttgcgg agaagaattg
    33121 acgaaaaaaa ctgcagaaag tctacttgat cgattcccaa atgcacggat atataatact
    33181 tacggtccaa cagaagcaac tgtagctatt tcaagtattc agattgacca gcatgtacta
    33241 gatagttatg accgactacc gattggctac gtaaaaaaag acacgaaagt ttctattgtc
    33301 caagaaggta aacctgtttc aaaaggagaa acgggtgaaa tcattattgc aggaccaagc
    33361 gtatcaaaag gttacctgaa caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat
    33421 gacttagcaa gttaccatac aggggatgca ggtagactag atgaatatgg gcttttattt
    33481 tatgaaggac gtatggatca gcaggtcaaa ttacatggat accggattga actcggggat
    33541 atcgaacatt atctgctcca agacaaccga atcaaacaag ctgtcgtcgt accaaaatac
    33601 caaggaacaa aagtacagca gctggttgct tttgtcgtat tagaaaacaa aacgaaacag
    33661 ccggattttc agttgactaa atctatcaaa gaacaattac tagaagttgt aatggattat
    33721 atgattcctc agaagttcca ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc
    33781 gatcgaaaaa aattaacagc tgaggtgaat cctgaatgat gttacctcat atgatccctt
    33841 atgcagagcc gttttatttt tttcttttag ccattgcgct tgtaccgatc gttctatctt
    33901 tattgatatg ggaaaaaaga ttaccagttt atcaatcagc agtgacactt ttctttttat
    33961 ttgtcagctt tggaggtaat tattggcaac agggagcggc gctgattggg tatgttttgt
    34021 ggcagacgat acttgtatgg ggctattttg tttatcggca aaggaaaaat cagacaggat
    34081 ggttttttgc cgccgttatt ttagcgataa ttccactgtt tttaacgaaa gtgactcctt
    34141 tttttgaaca tggaaaagca tcgttacttg gttttttagg aatctcttat ttaaccttta
    34201 aatctgtaca aatgatcatg gaaataagag atggcatgat caaaacatat aagccatttc
    34261 gttatatcca gtttctgctg ttctttccaa caatctcttc tggaccaatc gatcgctata
    34321 ggagatttga aaaagatcag ctccatccgc ctgttcgtga taaatatgtt gatcttttgg
    34381 gaaaaggcgt ccaaaatatt tttgtcggtt ttttatataa attcattatc ggttattatt
    34441 ttggtcaagt actgcttcct gtagtgggac gcattgcctt acagcatggt ggtatttcat
    34501 gggcattagt aggttatatg tacgtgtaca gtatgtattt gttttttgat tttgctggct
    34561 atagcttatt tgcagtcggt acaagctaca tgatgggata tcagacacca atcaatttca
    34621 ataaaccttt tttatcatgg aatatcaaag aattctggaa tcgttggcat atgacgctgt
    34681 ccttttggtt ccgtgattat gtatatatgc gattgatgtt cttcatgatg aagaaaaaat
    34741 tttttaaaag caagatcgta atgtcaaata tcggttattt tgctttgttt cttctaatgg
    34801 gtatatggca tggattgacg tggtactaca tcgtatatgg actgtatcat gcttgtctga
    34861 tctgtctgac agatgcatgg atccgattta agaagaagca taaagagcag ttgccttcta
    34921 acaaatttac gcatggctta gccgtatttt tgacatttca agcagtatgt tttagcttcc
    34981 tgattttttc aggattttta gataaacttt tttttagcta acgaatagtg acatattaat
    35041 ttatataaca aaggagaaaa ggaaaatgga agaacaaatt ttatcaatct tagaagaaat
    35101 aacaggaaca gatgaagtaa gaaaaaatcg tgatattgat ctatttgatg aagggttgct
    35161 tgattcttta gcatctgttc aattattagt cgagctagac ggagagctgg atatccaagt
    35221 ccctgtatct gaattcgagc gagaggattg gagcacaccg aataaaatca ttcaacaagc
    35281 aactgcattg aagggttgag tatgaaaaag aaactttttg ctattttcgg cccggttgtt
    35341 ttagctgttg gcttactgtt tgtgtttttt acctcctcct atcaagtaga tatctctaat
    35401 ccagaaacga ttagacgtgc ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg
    35461 ataaaaaatt atgcattatc tgaaaaacaa tatattcctt tttttggctc aagtgaatta
    35521 agcagaatca gtccatttca tccgtctgtt ctggcagaaa aataccagcg aaactatcgt
    35581 ccatttttgc tgggagcacc cgggacacaa tcgttaagcc aatatatgat gatgcgttct
    35641 gcaggagatg ctatgaaaaa caagaaagtc gtctttatta tttctcctca gtggttcgtt
    35701 aaaaatggtg taaaaactga ttactttaat acgtattatt ctgaacttca aacatacgac
    35761 tggttgtttt caatgaagaa agtaacgcct gctgatcgct atttagctag aagattatta
    35821 actttctcaa aagtaaaaga gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa
    35881 gggaagctac ctttacctga atctttgaat cagctgcgca gccaatggaa catgctgaag
    35941 agagaggatg aagtattcag caatattggg ttgaaagatc gacagcaaaa aatcgatcat
    36001 gagtcaaaac gattaccaaa acaataccaa gaaacagaat tatccatttt agcaaatcaa
    36061 attggagaac gagaaacgac gaataatcca ttcgggttga aaaacgattt ttatacgcat
    36121 cgtatccgag cccacgagcc agagctcaaa cagtcacaaa aaaattggga ttatcgtttt
    36181 tcgccagaat tttctgattt tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa
    36241 gtattgttca tcatcccacc ggttaacgag aaatggagcg attataccgg actatctcaa
    36301 gaaatgttac aaggatttgc caaaaagatc aaatttcaat taaatagcca aggattcaat
    36361 cgaatagctg attttgtcaa tcaagcgggt accaactatt ttatggaaga cacgatccat
    36421 ctaggctgga aaggctggtt ggctgcagat caacagatca gaccgttttt agaagaaaat
    36481 catattactg ctagtaagta tcatcttgac gatgcttttt ttagcaaatc ttggcaacat
    36541 caaataccag ataaactaca attaaaataa agaaaacagg cgagtgttgc tcgcttgttt
    36601 tctttatttt tgaatattct gaataaaaga taaaaatacc aagtttgcct gaacaaaaat
    36661 cataagcaat ggtataatca caatatgaat atagaaatgg gggaaaacat ttggatacaa
    36721 aagaagaaca gctacttatt gatttaactt ctgcaaaagg agtacctgga aatgaagaag
    36781 aggtaagaga agtattcaga gaatatgcca aaccattcgc tgatgatatt ttttatgacg
    36841 gtttaggcag tgtgattgcc aaacatggtg ctggcggagg ccccaaggtc tttatttctg
    36901 gacatatgga cgaagttggt tttatggtaa caaaaataac cgaaaaagga ttcttggaat
    36961 tccaaactct tggaggttgg tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa
    37021 cacgtgaagg gaaaatcgtc cacggtgtga ttggttccaa accaccacat gttttaacac
    37081 ctcaagtaag aaataaacct tatgaaataa aggatatgtt cattgatatt ggcgcatcaa
    37141 gccaagaaga agccaaagag tggggcatac gtcctggaga tatggtcact ccatatattg
    37201 aatacaaacg aatgaatggc agtaaatatt tactggcaaa agcatgggat aatcgtatcg
    37261 ggacagctgt ttctctgcga gtattagaga atctatctaa agaagcacac ccaaatgttt
    37321 tattcgcagg aagcgatgtg caagaagaag taggcttgcg aggtgctcga acaagtacac
    37381 accttgtaaa tcccgatatt gcttttgctt tagatactgg tacagcagga gacactccag
    37441 gaatgacacc aaaagaagca gattcgattt taggaaaagg cccacagatt ttgatttttg
    37501 atgcatctat gatcccgcat aaaaaattat tgaattttgt tattgatgta gcagaggaaa
    37561 tggatattcc attccaatat acagtgattt ctggcggagg aactgatgca ggacaaatgc
    37621 atttgacaag agatggtgtt ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt
    37681 cccatacatc agtgattcat gaagatgatt atttgaatac agtgaaacta gtcacagaat
    37741 tagtgaaacg gcttgatagt gaaacagttg aaaaaattcg gagttatgaa taatgaataa
    37801 tagaatacct atttctgaat atcggaaaaa agcacgagtt tctttggaaa atcaatgggg
    37861 aatcaatact tggcttgttt tcttatctat aatatttacg agcgtcatcc agaacttttt
    37921 tgacggtttc agtccttttc cggaagaaag catgcagcag cacttattga gttttctagt
    37981 cgataattta cttttattcg cattcaacta tgcattgttt tacattgcgc tagtagttgt
    38041 acggggtggc agaccaaaaa gtaacctttt attttcagtt ttccaaagaa agtattttgg
    38101 ttcgatactt ggcatcaatt tgatcaattc agtcatcaac tggctactca acagtttaat
    38161 ctttctgccc atgtttttaa tcagcggagt gagtacttat atgcagctgc ttttcagttc
    38221 aaatgcggta cctacgggtg tcatgccaga agattttctt gatatcagct ttgttttatc
    38281 gatgataggg atgatttttg tttcagcact catcatgagt attgttggcg gtttattcca
    38341 atttgctgca tggacaaagt tcgactatcc tgatttgtcg gttattcaat gtttgaaata
    38401 tggctggttt ttattgaaag atagaattgg tacgtatatt ttgttgcagc tgtcttttat
    38461 cggctggtat ttgctaggag caatcgcttt gttcatcggt cttttgtggg tcattgttta
    38521 tgtgaatgtg tcagttgcag aattttatga acaagcacgg attgaaaaag gaggtccaga
    38581 cgcctatttt acttagcatg taagcagttt attcatcaaa aaaataactt ttgtggtagg
    38641 ataaatcttt gagggggaat gtacgatgaa attaactgtt ttaggatgct taggagcgta
    38701 tccttataaa aatcaaggga caacaggtta tttgcttcaa tctgacgggt tcaatttgtt
    38761 gatcgatgca ggtagtgcta cgctgatcaa attacaagaa caccttgatc cacttgatct
    38821 agatgctgtg atccttagcc attatcatca tgatcatatt gctgaccttg gtgtattaca
    38881 gtattactgg caacttcatc ctaagagaaa tccggagaca ttgctttcaa tttatggaca
    38941 cacaaaagat tccattcact ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc
    39001 ttattttgaa gctgaagaat taaaaattgg tccttttctg attactttta tggaaacaat
    39061 ccatccagta gtatgttacg ccatgcgtat cgtagaagaa acaacgggga aagtattcgt
    39121 atttacaggt gattcaggtt atttggaaag tttcgctgat tttgcgaaag atgctgattt
    39181 gtttctagca gatacctatc tttttgaagg aaatgaacat catcatgccc atttcacttc
    39241 aaaagaagct ggagaacttg cgaaatcagc taacgtaaag aaacttgtac ttacacacct
    39301 acctcagttt ggtgatttag aacaattgaa aaatgaagcg caaaaggcag caggcaaagg
    39361 gattgatata gaacttgctg cagttgacaa agtgtttaac atttaggagg aagaacatga
    39421 tttttgtacc aaatgaaaac cacgacccaa gaatcaattt agcaattgaa caatatttgc
    39481 tgcaagaaat gcctttagat gaacctattt tgctctttta tatcaatgaa ccttcaatca
    39541 tcatcggcag aaaccaaaat acgcttgaag aaatcaatcg agattatgtg gaagaaaatg
    39601 gcattcatgt tgtccgtcgc ttaagtggag gcggagctgt ctaccatgat tttggaaacc
    39661 tgaactttag ttttatcatg cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa
    39721 cacagccaat catccaagca cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc
    39781 gaaatgatct agtcattgat ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg
    39841 gtcggatgtt tgctcatgga acaattatgt tcgactctga tatcaatgaa gtagtcaatg
    39901 cattaaaagt aaaaaaagac aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg
    39961 taacgaatat caaaccattt cttccagaag aaaagcaaaa aatgacaaca gaagaatttc
    40021 gtcaagaaat cttgttgaaa attttcggcg tagagtcagt agaagaagta aaaacgtacg
    40081 aattgactga tgaagattgg aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt
    40141 gggattggaa ttacgggaag tctccagatt tcaatttctc tcgccaaaaa cgtttttcta
    40201 tcggttcgat tgaagtccat ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct
    40261 ttggcgattt cttcggacta ggagatatct ctgacgtgga aaaaaaattg gttggacaga
    40321 aatatgataa agcatctcta tcaaaaatag tcagccaaat tgatgtgaag aaatattttg
    40381 gtgcgataga accagaagaa ttgcttacct tactttattg aaaataaaaa atagcggaat
    40441 ccacatatat ggattccgct attttttata caggattcgg gtaatttcga gagagttgaa
    40501 cagtgatccg atcaatgaaa gtatcttttt cctgcgttag tgttcctgtg taacgatccg
    40561 ctacgtcttt catcatcatt aagtcaaaac gttcctgatc atctaattct tctgtgatgc
    40621 ctcgactaaa taggtttgta tcgtaatgtg cactatgttc gcaacaaatg acgaaatgtt
    40681 cctcttgttc gtgtatcgac aaataaattt ttcgatacag tggattttcg atggcatgag
    40741 tttctctaaa ggaatgctct aaagcgtgga tcaaaagttg agccagatcg tcatcgttga
    40801 taggaagttc ttcgggaaga tctgcttgga aggttatctg aatattccgt ttggcagcta
    40861 acttctggta acgcgctaag ataagatttg tcacctggtg gttgcaaaaa gaacacagca
    40921 tttcgaaatt ttttgcttct tctaatatct tttccacata gtttttagct tttgtttggt
    40981 ccgtatgcat taaatcttgc agattctcca tctcttggac aaattccagg cgaagtgtat
    41041 ttacttcttg gatatgctgt cgaagctgct cataatgttg ctccaacaaa tcatttttga
    41101 tttttaagaa atctacttgt ctccgatctt tgtcggtatt taagatgtga tcgattagat
    41161 tgtagctgat aataacgatt agaatgataa tatataaaat cgtagaccaa ttgatcgttg
    41221 tttgatagat acctaatgcc gtttggatgt aaatcaagca atggataatg gctaccaaga
    41281 cgatccaagg tgtacaagct acataaaaac ggttattctt atatgcttcg ccaatgcatg
    41341 caagtgaagt gactaaagtg cttataacac tagcaatatt agcaatcggc attaattcag
    41401 gaagttctgc aggagtaaaa gttgcataaa gcagaacgcc gataaaaatc aaaagttgga
    41461 acgtacaaaa aattccgtac cattttttat agtaaaacat tcggctccaa tagtagcaga
    41521 tgatgaaagc tgacgttaag ataataaata aatgagataa aaaagaatga acgaatggtt
    41581 caaaaagaat accacttaaa atatcttctg atacagcttc aaatcctaat gccaaagcaa
    41641 agcaacttaa gagaataagt gagtattcaa gacgtttttg tttatacatc aagtagatcg
    41701 tatagaaaaa ggtccctata gcgataaaaa cagcgattat catcgtcaat acttgcggca
    41761 ctgattgtga ataaataaaa ctaatcaatg gtccagttgt tcctagataa actttcggtg
    41821 gcagtccagc atagttctga taaggagatg agactgtaag ggtcaatttt tttccgatat
    41881 aatttgctgg taaatctatc acagccaatg agagtccggg attgctttct tcctgataat
    41941 ctttccgttg gtataaaatc ttttcgccta gatatacagt aatccattga tggtttcctt
    42001 tgattagtaa atttgcttcg tccaacggtt tttcaagtgt cgtttccata atgaatgttt
    42061 cgtctttatc cacggtagga atcgtacgag tgtacttgct gtcaaactta aaatcaggat
    42121 catcttttgt atagaaaacc cacgaatctt ttatttcttt catttgattg ctgtccgcag
    42181 gagaaaaaat aaaataaaag aaataagcaa ttcctgttaa aaataacaat
//
LOCUS       DACEKH010000012        41427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEKH010000012 DACEKH010000000
VERSION     DACEKH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.012"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(174..1565)
                     /locus_tag="IW662_000590"
     CDS             complement(174..1565)
                     /locus_tag="IW662_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ0865439.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1778..2164)
                     /locus_tag="IW662_000591"
     CDS             complement(1778..2164)
                     /locus_tag="IW662_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ0865440.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2220..2843)
                     /locus_tag="IW662_000592"
     CDS             complement(2220..2843)
                     /locus_tag="IW662_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ0865441.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2907..3575)
                     /locus_tag="IW662_000593"
     CDS             complement(2907..3575)
                     /locus_tag="IW662_000593"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0865442.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3587..4027)
                     /locus_tag="IW662_000594"
     CDS             complement(3587..4027)
                     /locus_tag="IW662_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865443.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4086..4757)
                     /locus_tag="IW662_000595"
     CDS             complement(4086..4757)
                     /locus_tag="IW662_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0865444.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4942..5340)
                     /locus_tag="IW662_000596"
     CDS             complement(4942..5340)
                     /locus_tag="IW662_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ0865445.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5337..6110)
                     /locus_tag="IW662_000597"
     CDS             complement(5337..6110)
                     /locus_tag="IW662_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0865446.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6107..6790)
                     /locus_tag="IW662_000598"
     CDS             complement(6107..6790)
                     /locus_tag="IW662_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0865447.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6957..7844)
                     /locus_tag="IW662_000599"
     CDS             complement(6957..7844)
                     /locus_tag="IW662_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0865448.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7841..8710)
                     /locus_tag="IW662_000600"
     CDS             complement(7841..8710)
                     /locus_tag="IW662_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0865449.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8847..10133)
                     /locus_tag="IW662_000601"
     CDS             complement(8847..10133)
                     /locus_tag="IW662_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0865450.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10406..10984
                     /locus_tag="IW662_000602"
     CDS             10406..10984
                     /locus_tag="IW662_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0865451.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10984..11913
                     /locus_tag="IW662_000603"
     CDS             10984..11913
                     /locus_tag="IW662_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0865452.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11910..13646
                     /locus_tag="IW662_000604"
     CDS             11910..13646
                     /locus_tag="IW662_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0865453.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13709..14545)
                     /gene="pheA"
                     /locus_tag="IW662_000605"
     CDS             complement(13709..14545)
                     /gene="pheA"
                     /locus_tag="IW662_000605"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ0865454.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14542..15051)
                     /locus_tag="IW662_000606"
     CDS             complement(14542..15051)
                     /locus_tag="IW662_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ0865455.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15044..16339)
                     /gene="aroA"
                     /locus_tag="IW662_000607"
     CDS             complement(15044..16339)
                     /gene="aroA"
                     /locus_tag="IW662_000607"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0865456.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16352..17443)
                     /locus_tag="IW662_000608"
     CDS             complement(16352..17443)
                     /locus_tag="IW662_000608"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ0865457.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17460..18626)
                     /gene="aroC"
                     /locus_tag="IW662_000609"
     CDS             complement(17460..18626)
                     /gene="aroC"
                     /locus_tag="IW662_000609"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ0865458.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18628..19692)
                     /gene="aroB"
                     /locus_tag="IW662_000610"
     CDS             complement(18628..19692)
                     /gene="aroB"
                     /locus_tag="IW662_000610"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ0865459.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19725..20747)
                     /gene="aroF"
                     /locus_tag="IW662_000611"
     CDS             complement(19725..20747)
                     /gene="aroF"
                     /locus_tag="IW662_000611"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ0865460.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21406..22380)
                     /locus_tag="IW662_000612"
     CDS             complement(21406..22380)
                     /locus_tag="IW662_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ0865461.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22361..24244)
                     /locus_tag="IW662_000613"
     CDS             complement(22361..24244)
                     /locus_tag="IW662_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ0865462.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24262..26487)
                     /locus_tag="IW662_000614"
     CDS             complement(24262..26487)
                     /locus_tag="IW662_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0865463.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26602..27636
                     /locus_tag="IW662_000615"
     CDS             26602..27636
                     /locus_tag="IW662_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0865464.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27703..28716)
                     /locus_tag="IW662_000616"
     CDS             complement(27703..28716)
                     /locus_tag="IW662_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0865465.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28794..28982)
                     /locus_tag="IW662_000617"
     CDS             complement(28794..28982)
                     /locus_tag="IW662_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ0865466.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29422..29550)
                     /locus_tag="IW662_000618"
     CDS             complement(29422..29550)
                     /locus_tag="IW662_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0865467.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29553..30926)
                     /gene="rlmD"
                     /locus_tag="IW662_000619"
     CDS             complement(29553..30926)
                     /gene="rlmD"
                     /locus_tag="IW662_000619"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0865468.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31037..32092)
                     /locus_tag="IW662_000620"
     CDS             complement(31037..32092)
                     /locus_tag="IW662_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0865469.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32102..33532)
                     /gene="gatB"
                     /locus_tag="IW662_000621"
     CDS             complement(32102..33532)
                     /gene="gatB"
                     /locus_tag="IW662_000621"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ0865470.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33529..35001)
                     /gene="gatA"
                     /locus_tag="IW662_000622"
     CDS             complement(33529..35001)
                     /gene="gatA"
                     /locus_tag="IW662_000622"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ0865471.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(35001..35306)
                     /gene="gatC"
                     /locus_tag="IW662_000623"
     CDS             complement(35001..35306)
                     /gene="gatC"
                     /locus_tag="IW662_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ0865472.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35412..37448)
                     /gene="ligA"
                     /locus_tag="IW662_000624"
     CDS             complement(35412..37448)
                     /gene="ligA"
                     /locus_tag="IW662_000624"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ0865473.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37465..39699)
                     /gene="pcrA"
                     /locus_tag="IW662_000625"
     CDS             complement(37465..39699)
                     /gene="pcrA"
                     /locus_tag="IW662_000625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ0865474.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39854..41413
                     /locus_tag="IW662_000626"
     CDS             39854..41413
                     /locus_tag="IW662_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ0865475.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT        11484 a         8978 c         7419 g        13546 t
ORIGIN      
        1 ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttattc acggtgtttc
       61 agaagtagct tctcggaaat aagccgaaat ttcacaaaaa tttgagaaac tatttttagg
      121 aaacttcttc ttatttctta aagctgacca cttctatcac aacctctttt tgactatgca
      181 ttcacatcga tcactttaga atcatcattg atgacatcgc gatctaaatc tttgatgcgt
      241 gcacttgtca cgataccagc taccatgcta ccgctgacgt ttactgctgt acgtgccata
      301 tcgattaatg gttcaacaga gataactaac ccaacgattg ctactggcaa gttcattgcc
      361 cctaaaacga tcagtgaagc aaatgttgcg cccccaccga ctccagctac accaaaagaa
      421 ctgattgtta caattgccac aagcatcaaa ataaattcca aactaaagac attcactcca
      481 gctgctggtg cgacgatcgt tgccaacata gctggataaa cgccagcaca gccgttttgt
      541 ccaatagaca aaccaaaact tgctgagaaa ttagctgttg catcatctac tcctaaagct
      601 tttgtctgag tctcgatatt caatggcaaa gctccagcac ttgaacgtga agtaaacgca
      661 aagctcaata ccggaaaggc ttttttgaaa tagttgattg gattgacttt tgcaccaatc
      721 aaaatcagtg agtggacaat caaaacagca agcaatgcta cgtaagaagc aacgacaaat
      781 ttacctaaat tgacgatagc tgcaaaatca cttgttgcca aaacgtttgt catcaaagca
      841 aatactccat aaggtgtcaa acgcaaaaca agcgtcacga tccgcataat gattttatac
      901 agactgtcga ctaaatctga aaagaatgct gcttcttttg gcgcttttct tttcacgcct
      961 agataagcga ttccgacaaa tgccgcgaaa ataacgactc caatcgtact tgttgcacga
     1021 ctacctgcta aatcagcaaa aacgttttta gggataaagg ataaaatctg ctgtggaatc
     1081 gataagtttt caactgtttc ctgacgtgtt gccagttcag agattcgtgc tgtttctgca
     1141 gctccttgtg tgaatttagc tccttggagt ccgaacagca ttgttgaacc aattccgatc
     1201 aatgctgcaa tagctgttgt tccaagtaaa gaagtcaata cagtcgcact aattttccct
     1261 agcttttcag aaactttcat ttttgtgaat gcaccaatga tcgaaataaa cactaaaggc
     1321 ataactagca tttgcaagaa tgatacatat ccttgaccta caatcccgat ccattccatt
     1381 gattgagtaa cgactttgtc gtctacacca aagatcaatt gaagcagacc gccaaagacg
     1441 ataccaactc ctaatgctgt aaagacgcgt gttgaaaatt ttgtatggcg cttttgcata
     1501 cgatagaaca catataataa tgccgcaaaa actagaacca ctaaaactgt gatgagtgtt
     1561 gtcatttttc tcctcctcat attattaatg acaagtccat cgaaggaagt aaaggttccc
     1621 gagaatgatt cttcaaccat cctcctgaaa aaattttttt cagtgacttt tatattttag
     1681 acgattttta tcgaaatgac attctttttg cttacaatta gtaataaaaa aacatacctg
     1741 tgatagcttt aatctatcgc aggtatgttt ttactgtcta ttcttgtttt tctgattgaa
     1801 aattcaattt taccccaggc tgatctaagg cgatctctgt cacctcataa gacattcggc
     1861 gtacgatatc gaataacggt gctactagct cgtccacttc ttcttgtgtg acatcatttt
     1921 gaccttcaat tttccgattg aggatatgat tgatctgact cacacttcca gataaaacat
     1981 atgtatcaaa cactaaacgg aactctaagc gtgctcctac gatgctatgt tctttcggat
     2041 agttttcgat cgtttctttt aaaggcgaga agcctacttt caaatctgtt tctacttctt
     2101 ggttcggcaa tctcatatca taatgaaatg cttctacaac ttctttttgt cttctgattt
     2161 ccatataatt ttatcactcc tttatgaaaa ttataacata tctcctttga aaggggatac
     2221 tactttttaa tgatgctctg caaggtaagg atttcattca gatttcctac cacataatct
     2281 gccgggatct cacccagaaa atgatcgatg tcgtaaagac aagtagcgac tcctgcattt
     2341 ttccctgctt cgatatctaa acgacgatcg ccgatcatca tcgtgtctgt tctttctaaa
     2401 tgaaaagtat cgatcaaata atttaaagat gctggatcag gtttacgagg aaagtcctga
     2461 tcgatcccta ctacttcctc aatcagatgt gccaatcgat gttcttttaa caggccccat
     2521 gttgattctg tcaaacggtg ggtcaggatg aaatgacgac cgccgttgtc ttttagcatt
     2581 tccagtactt gtttcgtttc tggaaatgga cgggaaactt ccgtactttc tttttcatat
     2641 cggtgaaata attcattaaa ctctctggca ttcaagtttg attcattgat caaataacta
     2701 gtagaatact ttttcatttt gaagtagatt tcttttttgt ccatactgat accaaaatct
     2761 tttagtgctt gccatgcacc atctaccatt gcgggatacg tatcaaacaa tgtaccatca
     2821 aagtcccata tataattttt catgttcctt ttgttccatt cctttgcttc ttttttctat
     2881 ctctcacttt tagataaagg tgttctttaa agtatttttt tgtccagaat tgtcaaatca
     2941 tctgctaatg tatactggat tggttgtgca tttgggatat cgatcgtatc catttgatct
     3001 tcaggaacat cttctaaata ttttgtcagt gctcggatgc tattaccatg acccacaact
     3061 agtaaatctt gccgatcttt tagttttggt gctagatgat cttgccataa gggaatgatc
     3121 cgattcactg tcatttccag actttctccg tatgggatca aatgcggatc caagtgatca
     3181 tatctgcggt caaatctatt ttctgcggtt aacggcggaa cttccgtata tcctctgcgc
     3241 catgttttta cttgatcttt tccgtatatt tctttcatct tatctttgtt tttacccaca
     3301 agatccccat aatgccgttc atttagacgc catgttttta ttataggaag ccataactga
     3361 cccatttctt ctagtgcgac ttgtccggta agaatcgcac gtttcaaaac agaggtataa
     3421 acggcatcga attgaagtcc ggcttcaagc attttttgac cggcaactgc tgcttgttct
     3481 tttccttttt ctgttaaggc gacatctagc catcctgtcc agtaattttc aaaatttgct
     3541 tcgctttcgc catgacgtaa taatactaat ttcattcttt tctcccttaa acattcgttt
     3601 cgaattttat accacatgtt ttgcatgtca cacgtattcg tccttttcct ttaggtgcac
     3661 gctgcttttg atgacaattc gggcactcaa aaaaagtata actttttcga tccattttga
     3721 cttgcttttt gttttttatc tgatgatatt tttctttgac tctatcaaga tatttcaaat
     3781 aacgttgatt ctcatttagt ctggggtaga ttttttttga taggaaacga aatatcatcc
     3841 atccgaaaaa gacgatgaac aacaattgtc cgattctata gggcaaccaa cgacttagca
     3901 aactaaagac aagcgcagcg atcaatagac cgcgattcaa ctgatcaaat ttcgcatatc
     3961 ttccctgcat aagtttgtga tagttctgct gaaatcgtat tagcctttct aaccatgttt
     4021 gcatcatatt atctcctcca tcatctaata tttattatga caaaaaaatc caaaaagaaa
     4081 aagccttaat tggccttttc cattgcaact tggtgaagga cattccgatt tctggtaaag
     4141 tcaatcacaa tcactttctt tttatcttct ctgttgacta ctcgaaatgt gtccgtcaat
     4201 ggaagaaaca gcttcttaga tggtagctgt tgcatacgta taaaacgatg gatcatcgta
     4261 atcaattgag gaaattgaat agacgtgtga agcagacctg gatatttttt caacagctgt
     4321 gtcgttttga tcagattccg ttttttactg atcactttta aaaagctttt tagaatcttt
     4381 tgctgacgtc taaaaataga aaaagaattt tcgtcatcta tttgataacg catttgtttt
     4441 tgcagttctg ctgcagtcag atggatcttc ccatcgtttg tatcgatcat taaaccttct
     4501 ggaaacagtt caaccactgc tcgatcccaa gattcaagag aaagctcgat ataatctttt
     4561 actggtaatt ccaattctac cttaaaaaag tgcaaaacag ccgacagccc ttctgcttga
     4621 tagatttctc ctaatgttag cccctctaga tggaactctg gtgaaaaatg cagcaatgtt
     4681 ttattttcct gcaattgaac atatagaaat gtagatcgct caatcagagt atcttttaaa
     4741 acaactaaaa aattcatttt tctccccctt ttttcatcat ttttaaaata gcatattttg
     4801 aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc ataagtattc
     4861 acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt gtttgtgttt
     4921 atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat tttcatctaa
     4981 aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac caatgacttt
     5041 gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga tgccttgacc
     5101 aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca caatatcttt
     5161 ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag aacctcttcc
     5221 atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa catcttcgcc
     5281 cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa gatctttcat
     5341 ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc atcttttcgt
     5401 ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat cattgcaacc
     5461 acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat tgccacgctc
     5521 attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt atcaaaagcc
     5581 attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc gtatcctggg
     5641 atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa tcctactgct
     5701 gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct cccctcgctc
     5761 aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc tactaagaac
     5821 cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc aatagcactg
     5881 cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa aaggatcaac
     5941 ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt gcgcatacga
     6001 aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc caaaatgact
     6061 tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat tggaagatca
     6121 cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc attccaaaac
     6181 tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca agggccaaga
     6241 taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca atcggcataa
     6301 attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata gccatcaatt
     6361 gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat actccagtgc
     6421 cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat atatcatata
     6481 cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc ccgatagcaa
     6541 atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc cctactagat
     6601 ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt ccgatcatca
     6661 tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt gcaaaaacaa
     6721 acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat
     6781 tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc
     6841 gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca
     6901 aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat
     6961 tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca
     7021 gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa
     7081 gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact
     7141 aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag
     7201 gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct
     7261 aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag
     7321 atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa
     7381 accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga
     7441 accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc
     7501 tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg
     7561 atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga
     7621 aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta
     7681 ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt
     7741 aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg
     7801 acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta
     7861 ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg
     7921 ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg
     7981 tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca
     8041 atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg
     8101 tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct
     8161 ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc
     8221 aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg
     8281 gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata
     8341 gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg
     8401 ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg
     8461 ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt
     8521 ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat
     8581 tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca
     8641 tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt
     8701 tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa
     8761 gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc
     8821 gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt
     8881 gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct
     8941 acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc
     9001 catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa
     9061 gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg
     9121 cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct
     9181 ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt
     9241 tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct
     9301 gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat
     9361 tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa
     9421 agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc
     9481 tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca
     9541 aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct
     9601 acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct
     9661 gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat
     9721 attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca
     9781 atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa
     9841 tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc
     9901 agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc
     9961 tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag
    10021 acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa
    10081 cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc
    10141 cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa
    10201 aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca
    10261 aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt
    10321 ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa
    10381 gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca
    10441 gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat
    10501 agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat
    10561 cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa
    10621 acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt
    10681 taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga
    10741 tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag
    10801 cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca
    10861 aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc
    10921 gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca
    10981 ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc
    11041 ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt
    11101 caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc
    11161 aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga
    11221 aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca
    11281 agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag
    11341 aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca
    11401 atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa
    11461 ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat
    11521 ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa
    11581 aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa
    11641 gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc
    11701 atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt
    11761 aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc
    11821 ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct
    11881 tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct
    11941 ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt
    12001 tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg
    12061 tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat
    12121 tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac
    12181 atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa
    12241 tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg
    12301 gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga
    12361 gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc
    12421 gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg
    12481 tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg
    12541 tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat
    12601 caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg
    12661 aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta
    12721 cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga
    12781 ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg
    12841 atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg
    12901 gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga
    12961 tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga
    13021 agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata
    13081 atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag
    13141 gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc
    13201 cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg
    13261 gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga
    13321 atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc
    13381 aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg
    13441 ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa
    13501 tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa
    13561 cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta
    13621 aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt
    13681 taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg
    13741 gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct
    13801 ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc
    13861 tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg
    13921 ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt
    13981 ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct
    14041 ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg
    14101 tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg
    14161 cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact
    14221 gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat
    14281 tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat
    14341 gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa
    14401 cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat
    14461 acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt
    14521 ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat
    14581 cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga
    14641 aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg
    14701 aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta
    14761 aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc
    14821 accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt
    14881 tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc
    14941 ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc
    15001 gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc
    15061 ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt
    15121 cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac
    15181 cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga
    15241 tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca
    15301 ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga
    15361 ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa
    15421 tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg
    15481 attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat
    15541 ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg
    15601 gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta
    15661 tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga
    15721 tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa
    15781 cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg
    15841 cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga
    15901 ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt
    15961 ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa
    16021 aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt
    16081 caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct
    16141 cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat
    16201 cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga
    16261 tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta
    16321 tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat
    16381 tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca
    16441 gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg
    16501 cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa
    16561 ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt
    16621 cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg
    16681 ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc
    16741 tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa
    16801 agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat
    16861 tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat
    16921 gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt
    16981 tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata
    17041 gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga
    17101 caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat
    17161 ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg
    17221 ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat
    17281 cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac
    17341 tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag
    17401 caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt
    17461 tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa
    17521 tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca
    17581 acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt
    17641 tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg
    17701 accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta
    17761 ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag
    17821 cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata
    17881 ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct
    17941 aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt
    18001 ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt
    18061 acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct
    18121 gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga
    18181 attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt
    18241 tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt
    18301 cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca
    18361 gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa
    18421 ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc
    18481 atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct
    18541 tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg
    18601 gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc
    18661 tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg
    18721 gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag
    18781 atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc
    18841 agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac
    18901 tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt
    18961 atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt
    19021 tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc
    19081 gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat
    19141 gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc
    19201 aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt
    19261 gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg
    19321 aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat
    19381 tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc
    19441 gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag
    19501 gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc
    19561 aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga
    19621 tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg
    19681 tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga
    19741 atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt
    19801 tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg
    19861 atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa
    19921 attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat
    19981 gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt
    20041 tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat
    20101 tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca
    20161 gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac
    20221 tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat
    20281 tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat
    20341 ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc
    20401 attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc
    20461 acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg
    20521 aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca
    20581 tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact
    20641 cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact
    20701 ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct
    20761 ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca
    20821 ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt
    20881 atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca
    20941 tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc
    21001 tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt
    21061 gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt
    21121 cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta
    21181 cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt
    21241 ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca
    21301 tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag
    21361 ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa
    21421 gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca
    21481 gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc
    21541 tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc
    21601 ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt
    21661 atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat
    21721 gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc
    21781 ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa
    21841 aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc
    21901 tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga tttggtagga
    21961 aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt
    22021 ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt
    22081 tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca
    22141 cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa
    22201 aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc
    22261 gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc
    22321 gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat
    22381 aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat
    22441 tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt
    22501 ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc
    22561 ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg
    22621 aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa
    22681 ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat
    22741 cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt
    22801 ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag
    22861 accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa
    22921 gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc
    22981 ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg
    23041 cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt
    23101 tgatctttgc attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc
    23161 gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat
    23221 tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat
    23281 actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct
    23341 gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt
    23401 ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca
    23461 gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac
    23521 atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa
    23581 ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg
    23641 cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg
    23701 ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca
    23761 accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta
    23821 gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg
    23881 aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat
    23941 gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa
    24001 tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg
    24061 tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact
    24121 gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat
    24181 gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt
    24241 tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc
    24301 accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg
    24361 cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg
    24421 gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt
    24481 aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata
    24541 atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg
    24601 cttttcttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat
    24661 agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc
    24721 tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt
    24781 catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa
    24841 cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc
    24901 agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc
    24961 ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca
    25021 tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg
    25081 ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct
    25141 acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat
    25201 catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat
    25261 ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt
    25321 gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc
    25381 ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc
    25441 ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc
    25501 acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag
    25561 gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct
    25621 gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg
    25681 gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg
    25741 atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa
    25801 tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc
    25861 tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc
    25921 cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat
    25981 cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc
    26041 cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa
    26101 gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa
    26161 gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg
    26221 agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac
    26281 atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac
    26341 tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga
    26401 aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt
    26461 atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac
    26521 ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa aattttttat
    26581 ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa ggcaaatgtc
    26641 tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt ggctcagact
    26701 actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag tactcgtaaa
    26761 tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa
    26821 gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc tcgacgtctg
    26881 catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg ggaagatcta
    26941 gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat taccgccttg
    27001 ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca agaggtcgat
    27061 atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt cttagaaaat
    27121 ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga attcggtggg
    27181 aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat gatcgatcat
    27241 cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca aggtgaggta
    27301 cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct tgtcgggagc
    27361 gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca aattggagaa
    27421 gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc ccctggcttg
    27481 accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat ggcattggag
    27541 cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg attgattgtt
    27601 agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga aaagccgctt
    27661 cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat acttattcac
    27721 aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat caaattggta
    27781 ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg gtacctttaa
    27841 ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg catttccgtc
    27901 agttatatac gttggcggaa attccttaga aacatggtca gccacactag cttctttcaa
    27961 ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg ttttgacgaa
    28021 aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat caacaggtcc
    28081 gcaataagaa atggttcctt tcagactatc ttttggaagt agatgtttga ttttcatttg
    28141 tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt gtgaacctgc
    28201 actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt tttcttcagc
    28261 taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt ctgaatcagg
    28321 cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta attttgtcgc
    28381 aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta cccaaaatag
    28441 aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat tctctttata
    28501 cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt gatcgaacgt
    28561 ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga atgctccagg
    28621 tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa tgattccgaa
    28681 gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt agttagtatt
    28741 aagcattctg attataccat agtgttattt cctatcaagc tctcctagat gatttaatga
    28801 ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca ttgttcttca
    28861 aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa gcacaaacga
    28921 tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg gtgctcttcc
    28981 atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt cagagacttt
    29041 ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat aggcacggac
    29101 attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg ccgccaaaat
    29161 cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg agaatatgaa
    29221 aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa tctgagacag
    29281 aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct tattggcaaa
    29341 tcgccaaaat aatgtagact aactaaaaag actaagaaac aataacaaaa gttaacactt
    29401 cttagtcttt ctttagcacg tctatttatt aaagatttta gggccttttc tcatgttttt
    29461 acgatcattc ttaggtgttt ccatttgttt ggatcggcct ccaccttgtt tctttttgat
    29521 caattctttc attttttctt tttggtccat ttttattctc ctttaacgaa tgaagcgact
    29581 aattcgatat gatgggtttg cgggaaaaga tctacaggct gaactttctt cacggcataa
    29641 cctgcttctg caaataactt agcatctctt gcaaaagttg ccggattaca tgagatatag
    29701 acgattcgtt ctggtgacat ttcgattgct gattcaataa aatgatgatc taatcctttt
    29761 cgtggagggt caactacgat cacagatggt ttgatccctt tttcttgcca ttttttcata
    29821 actttttctg cttttccgac ttcataatga acattttcga ttttattatt taaagcattg
    29881 aactgtgcat cttcaattgc ttctggaata acttccatcc catacacctt atccactctg
    29941 tctgctaaag acaagccaat cgtacctatt cctgaataag cgtcgatcac aatgtctttt
    30001 ttatctaact cggcaaactc gatggctgtt tggtacagat actctgcttg ttgagtattt
    30061 acttgataaa atgactttgc tgagattcga taggtttttc ctaatagctg atcttcgata
    30121 tagtctctgc catataaaac tttttctttg tctcctaaaa tcacatttgt tttttcttca
    30181 ttgatatttt ggatgaccga aaccacttct ggcaattctt catgaatagc ttcagcaatt
    30241 tccttggatt taaagaattt ttctttacga gtaactaaaa cgaccatcat ttcatgggaa
    30301 taatgaccac gacggataat gatgtgacga agctgtcctt catttttttc ttcattatat
    30361 cctcgtactt ggaaccgttg caagatatct ctgactactt tgattgcttg atcgatgaca
    30421 ggatcttgga tataaaaatc ttcgataggg actagatcat ggctattctt tttgtaaaaa
    30481 ccggtggcta actgtccatc gatacggcga acagggatct gtgctttatt acggtagccg
    30541 aatggttctg ccattccgac cgttggaagt acaggaactt ctggcatctt agcaatcttc
    30601 gccattacat gctctacttg tttttgtttg aagacaagct gttgttcata cttcaaatgg
    30661 gctaaaggtg cgatacctat gcgaagtaat gcattgtttt ctacaggctg acgatcaggt
    30721 gattgtgtaa taaatgtttc gacttttcca tatccaaact tattgcctgc ttttactact
    30781 agtatttcta cttcttctcc agggagtgca ttttcgataa ataatggata accatcgatt
    30841 tttgctaccc ccattccttc ataactcaag tcgacgattt cgactgtata tcgttcgttt
    30901 ttctttacta tttgctgttc tgtcatattt tgctccttta agcctatttt tcaatcctac
    30961 tgaactctac tataaatcag ctaattacac aactaaaacc ggcactgacc ttactacgtt
    31021 cagcaccggc ttttgattaa ttcttttgat taggtgtttc gtctgctatt tttccgtcac
    31081 cgtcgatatc ttcatccgtt aaacgttcga cttcttttac gaattctttg ctgacaacct
    31141 ctaattcttc ttcatctgta cctgtgattg cttcatcagg aatctgatcg gtattggcaa
    31201 agaactcaat gtgttgatga agattttgga aatgcatcgg tgcttccccg ccgtattcac
    31261 catctaagtt gatcattact ggtcgattga tatcatcttc tgtttcaacg aacaaactgc
    31321 ttgttttggt atagattatt cttggatcat cgacatgtcg accgccattc agcatgagtg
    31381 ctgcaatatg caagatctca aagatatttg ctgtcttcac aatgattaaa gaaaacttcc
    31441 catcatccaa tttggcatct ggcgcgatct gttcgaatcc tccaacagag ttcgtcagcc
    31501 ctaggaaaaa catcgaagca tttccgatat attccccttc atcatacttc aagcgcattt
    31561 tgattggctt tactcgaggg agcatctctg ctcctttcgc aagataagca agataaccaa
    31621 aaatactctt tagttctgat ggaacttcat aagtcaattc tgtcaaatag ccacctgcag
    31681 cgatattgat gaagtaactg ttatcagaaa ggccgatgtc catttttacg gtttggtgtt
    31741 tttttatcac ttctgctgct gctttaatat tatctctagg aatttttaat gctcgagcat
    31801 agtcgttcgt tgtaccagct gggatgatgg ccattttagg acggtgttca agcggagcga
    31861 ttccattaac cacttcgttg attgtgccat cgccaccagc tgccactact agatcaaaac
    31921 ctagttctgc gactctacat gcttcattct tggctgaatc tggttctggt gtagttgcat
    31981 aggcgctggc ttcaaatcct gcttcttcta atgcctgtag gatatccgcc aaattttttt
    32041 tcaataattc ttttcccgag gtggggttat aaattacgcg tgcttttttc attttcagcc
    32101 tctatcgttt cgctaattca tctttaagta gcttattcac gacaccagga tttgcttgtc
    32161 ctttagttgc tttcatgatt tgaccgacta ggaagccaac cgcacgatct tttccatttt
    32221 tgaaatcatc gacagattgt tggttgttat ccaaaatttc attaatgatt ggcagtagtt
    32281 tgttcggatc agataattgc acccaaccat ttttttcaac tacttcttgg gcttctccgc
    32341 catttgtgat caattcacgg aagacttttt tggcgatttt tgagctgatc gttccatcgg
    32401 cgatcaaacg aatcattcct gctaagttct caggtgtcaa ttttgtctca gctaattcca
    32461 gtttctcact gttcaagtaa gcagaaactt cacccatcaa ccagttagat gcttgcttgg
    32521 catctgcacc attttccaaa acagcttcaa aaaaatcaga catttctttt gtaagtgtca
    32581 ataccattgc gtcatactct ggaagtccaa actcacggat atagcgttcg cgacgagaag
    32641 ctggcatctc aggcaaacta gcttttactt tttgaatcca ttcgtcatcg atcacgaatt
    32701 tcgggatatc aggctctggg aagtaacgat aatcactaga cccttctttg acacgcatca
    32761 aaactgtttt attcgttgtt tcgtcaaaac ggcgtgtttc ttgttggatt tctcctcctg
    32821 aaagaagaac ttttgcttga cgtttttctt caaaagctaa accttttttc acgaaagata
    32881 aagagttcaa gtttttcaat tctgctttcg taccgaactc ttcttgtcca taagggcgaa
    32941 gagaaatatt ggcatcacag cgcatagagc cttcttccat tttcacatcg ctgacttctg
    33001 taaattggat aattgatcgc aatgcttcta gataagcgta tgcttcttct ggtgaacgca
    33061 tatcagcttc agaaacgatt tcgatcaaag gcgtgccttg tcggttcaaa tcaacgtaag
    33121 aataaccgcc aataccatgc atgttttttc cagcatcttc ttctaaatgg acacgttcga
    33181 tccggatttt tttcttttta ccttctactt cgatttcgat ccatccgtca tgaccgattg
    33241 gttgatcaaa ttgggaaatt tgatatgctt ttggattatc tgggtaaaag taatttttcc
    33301 ggtcaaaatg agtatcttta gagatttcac agtttaatgc caaagctgct ttcatcccga
    33361 attccagcgc agctttattc attactggca gcacgcctgg ataaccccag tcgatcacgt
    33421 ttgtattgct gtttggttct gcaccaaaat gagctggcgc tggagagaaa atttttgagt
    33481 tggtttttaa ctcgacgtgg acttctagtc caatgactgt ttcaaaattc attcgtcttt
    33541 ccctcctaaa atagctggtt gttgtttatg gaattctgtt gcttgttcaa acgcatatgc
    33601 cgcttgatac atcgtttttt catcaaaata attaccgatg atctgcaatc caactggtaa
    33661 tccttctgat aaacctgccg gtacagacat acctggaagt ccagccaagt tgacagggat
    33721 cgttaagatg tcactcatgt acattgtgat tggatcgtta atgttctcac cgattccgaa
    33781 tgcaactgtt ggtgaagatg gcccgatgat caagtcataa tctgcaaaga ctttttcaaa
    33841 atctcgtttg atcaatgtac ggacttgtcc tgcttttttg aagtaagcgt cataataacc
    33901 cgcgcttaag gagaatgtac ctaacatgat acggcgtttt acttcttcac cgaatccttc
    33961 tgaacgtgag ttgacatata catcatctaa tgatttcaca ttttcagagc gataaccgta
    34021 gcggatacca tcgaatcgtt gcaaattgga acttgcttct gatgaggcaa tgatataata
    34081 aacagccaca ccgtattttg aatgaggcaa gctgacttct tccacaacag cacctaatga
    34141 acggaatgtt tctgctgctt tcaatacagc ttcttttacg ccaggggcca caccttcgcc
    34201 taagtattct ttaggtaaag caatcttcat tcctttgatg tcgcctgtca gtccctctgc
    34261 aaaatcagga actgaaacgc ctgaagaagt accgtctttt tcgtcataac cgctgatagc
    34321 tgtcaatgct aatgcattat ctttcaccgt acgggtcatt ggtccgattt ggtcaagaga
    34381 tgaagcaaag gcaatcaaac caaaacgtga gacacggcca taagtaggtt tcatccctac
    34441 gatgccgtta aaagctgctg gttggcggat acttccccca gtatcactac ctaaagaaac
    34501 tggaatttgt ccagctgcaa ctgctgctgc agaaccacca gaagagccac caggtacttt
    34561 tgttgggtcc caagcatttt ttgttttctt gaaataagaa gtttctgtac ttccacccat
    34621 agcaaattca tccatgttta gcttaccaac agggatcata tctgcttgat agattttatc
    34681 catgaccgtt gcatcataaa ttggttcaaa attgtaaagg atcttagatg cagcggttgt
    34741 caagatatct tttgttacga tattatcctt gataccaatt ggaataccag caaggacgtt
    34801 gctttcatca atcccttttt catctaaagc ttttgcctgt tccaatgctt tttcttcgtt
    34861 caatgtaatg aatgcctcga cctcaggctc tgtttctttg attcgttcaa atgttgtttt
    34921 tgtcaaatct tgtacagtga tttctttcga tacaagcata tgatgcagtt cttctagaga
    34981 acgattgtgt aattctgtca ttatgcgcca gcctccccat tatcgatgat tgcgggtact
    35041 ttgataaagc cgtcttctgt ttccggcaca tttttcaata attcatcgcg gttccatcca
    35101 gcttgcgccc gatcttgtcg catcacgttt actgtttcaa ccacattcga cgtaaatgga
    35161 acaccttccg tatctacttc ttccagcatt tcaaccatat cgatgatttt acctaattgg
    35221 tcggtaaaac ttgcaagttc ttcatctgcg aacgcaagtt ttgctaattt ggcaacgtgt
    35281 ttcacttgtt cttcactaat cgccatacta ccccttcttt ctaaacgagt taaaaacttt
    35341 gctctctatt ttaacacaag agtgccgtgt ctgaaattgt ttattgaaat ttgtctgaaa
    35401 aaactcattt ttcattcttc tactgagcga cttttagcca atgcatctgc catttgatct
    35461 tctgtccaaa cttcgattcc taattcttgt gctttggtca gcttgctgcc agcatcttct
    35521 cctgctacca cgatatccgt ttttttcgat acgctaccag tcactttccc gccaagattt
    35581 tcgatcgtct cttttgcttc ttcacgagta aatcgtgtta gtttaccagt aaggactact
    35641 gttttttctt taaaaggaga ctcgacttct tgcaattggg catttcgaag ccctttgtat
    35701 tcgaaattga cgcctgcttg ttttaattca ttcatcaatt catggacttc ttcgttactg
    35761 aaataagtga ctacactgtc ggcaatgata tcaccgatcg tatcaagcgc aataatcgac
    35821 tcatagtcac ttttactcaa tgtttctaga tcaccgaaat gttctgctaa aatttttgct
    35881 gctttcgctc cgacatgtcg gattcctaac ccgaaaatca accgctcgac cgaattatct
    35941 ttgctgttgt cgattgctgt caaaatatta tttgctgatt tttcttttat tttgtccaac
    36001 gtcaataact cttcttctgt caatttgtaa agatcagcaa catctgctac tagtttttta
    36061 tcatacatct gttctagtac gcgcggcccc aacccatcga tattcatcgc atttcttgaa
    36121 acaaagtggt tcaaaccttc tttcatttga gcagggcatt taggattgat gcaccgtaag
    36181 gctacttctt catccaaatg gacaagttca cttccacaaa ctggacaatg tgtaggcaat
    36241 tggtattctt cgctgtcttt aggtcgttta tctaacacga cttgcgaaac ttctgggatg
    36301 atgtctcccg ctttataaat cagaactgta tctttcaagc ggatatcctt caacttaata
    36361 taatcaccgt tatgcaggct ggcacggctt actgtcgttc ctgctactcg gactggtgtc
    36421 atgatagctg ttggcgttac tacccctgtt cgaccgactg tccattcgat gttttcgatt
    36481 agcgtttcaa cttcttctgg cggaaacttg taagctgctg cccatctcgg tgctttgata
    36541 gtaaaaccaa gttgatcttg tagcgagaat tcattcactt tgatgacgat tccatcgatt
    36601 tcatagggca aatccacacg tttgtcatga tattcttcaa tgtatgacca cacttcatca
    36661 atcgaatgac atagacgttt ttcccgattc gtgtggaatc caattttttc tagctcttct
    36721 agcgcatcat actgggtttt tgcttgcata ggaccgaaat cagctactgt gtataaaaaa
    36781 gtgtccagat ttcttttcgc tgtgatttta gaatctaact gacgcaaaga accagctgcg
    36841 gcgttccgag gattcgcaaa gatatctttt ccctctgctt ctctgtcttg gttcaattgg
    36901 acaaatgacc gttttggcat gaaacattcg cctcttactt cgatgttcat tggttctttt
    36961 aatttgattg ggaccgaccg gacagttttt aggttttcag tgatgttttc tcctacagtt
    37021 ccatccccgc gagtcgcacc tctcacgaaa tttccatctt cataacgcaa agaaacggaa
    37081 agtccatcta tttttagttc acagcaatag tcaactacac gtcccactgc tttttgtaca
    37141 cgttgatcaa aagctatcag ctcttctttg ctgaaaacgt cgttcaaact gtaaagcgga
    37201 atatcatgag tgactttttc aaaaccttct aatacttttc cgcctactcg ttgagtggga
    37261 gaatcagacg tgatcaaatc aggatattgt tcctcgattg ctactaattc tgcgtattct
    37321 ttatcataaa catagtcttc cactgttggt ttatcttcta cgtagtattc gcgagaccat
    37381 tggttcagtc ttgtacgaag ttcttctgct cgtttagcag cttcgtcaat cgtcatttca
    37441 tgttccatca tttaccctcc actactaagc tttttcaatt ggcgcaaagg cagccaaaag
    37501 acgttttact ccttgacttg ggaaagccac atctagttct agatccttcg acgtcccgct
    37561 tactcggacg actgttccta gaccccattt tttgtgtttg actttatctc caggtttcca
    37621 gctgttattt tcccctccag tagcactttt agagggaaca accgctgtct ctggctgttt
    37681 atagctatat gctgtttttt tagcgaatac aggcgttttt ttaggcgtag gacgcatgcc
    37741 ttcgatttcc agtaactcct gatcgatttc ttctacaaaa cgagaaggtc ggttatattg
    37801 cgtccgtcca tataatgttc tggagaaagc atttgttaaa tacaacgctt cttctgctcg
    37861 cgtgatcccc acatatgcga gacggcgttc ttcctccagt tcgctttctt ccatcaatgc
    37921 tcgagataaa ggaaaaaccc cctcttctag accaatcagg aatacaactg gaaattctaa
    37981 tcctttggct gcatgcaaag tcatcaatgt cacttgggaa gcatcttctt ctaaattgtc
    38041 aatgtcggaa acaagtgcca gatcatttaa gaagaccgtt agtttttctt ctggtgcatc
    38101 agcgtcttct tcattttgct gttcgaattg tttgtcgaat tcttgtgtga ccgttaaaaa
    38161 ttcctccaag ttttcgatgc gtgcttgtgc ttctaatgta ttttggattt ttaagtcttc
    38221 tagatatccg ctacgatcca aaacctcttt cgtcaattct gtcactgtca agtatgggat
    38281 catctgagtg acttcttgga tcatctctcc aaatgcacct aattgctgtc ctgcttttcc
    38341 accgatattt gccagatcca cattttgcgc tgcttcaagc agtggccatt catgaagaga
    38401 agcaaaactg cgtagttttt cgatagatcc tggtccgatg cctcgtttgg gcgaattaac
    38461 gatacgctca aaactgattg aatcttgagg atttaccagc acattcaaat acgctaaaat
    38521 atctttgatt tctttacgat cgtagaactt gtgccctcca accattttat aaggaatatt
    38581 tgctttcaac agagtttctt ccattacacg agattgtgcg ttcgtccggt acaagatggc
    38641 aaagtcgccg taattccgat gattgcttcg aatctcttct tgcatccggt ctacgataaa
    38701 acgggtttcg tctcgttcat tatctgcacg gtagtaagtg atcttgtttc cttctttgtt
    38761 ttctgtccac aagttttttg gttttcgatt gctattgttt tcaatgactt ggtttgctgc
    38821 gctaaggatg tttttcgtgg agcgatagtt ttgttctagc aaaattactg cagcatctgg
    38881 ataatctttt tcaaaatcca agatgttctg catatctgca ccgcgccagc cgtaaatact
    38941 ttgatctgca tctcccacga cgcacaggtt cctaaatcta cctgccaata aattgactag
    39001 cgtatactgt gcgtgatttg tatcttggta ctcatctaca tgaatataat ggaatttgtt
    39061 ttgataatac gtgagtgaat caggatgttc ttcaaataat cgaatcgtat tcatgatcag
    39121 gtcgtcaaag tccatacatt gattatattg aagctctttt tggtaagcag cataacattt
    39181 tgccgcaatc tcttcgtaat aactgccttg catttttgca aaatcctgag gggtctgcag
    39241 gctgtttttt gcttgagaga ttgttcctaa tatacttcga ggatcatatt ttttcggatc
    39301 aatgttcagt tcttttagta tccgtttcat caaggtcagc tgttcagaag aatcgatgat
    39361 tgtaaagttc cggttatacc cgataaaatc aacatctctt cttaaaatac gcacacacat
    39421 cgagtgaaaa gtcgataccc agacatcttc gcctccagat gcgagaatcg cattgacacg
    39481 ttctttcatt tcttttgcag ccttattggt aaaagtaatc gctaggatgt tccaaggatt
    39541 gacttctttt tcctcaatca aataggcaat gcggtgtgtc agcactctcg tctttccact
    39601 tcctgcacct gccattagca gtaatggtcc gtcagtgtga agaacagctt ctttctgacg
    39661 ggggttcatc ccgtttaata attcagcttt ggatgacatt aacaacatcc tttcttttat
    39721 caatcttgtt aattatagca ttttcttggc tttcattaaa ctagctagtt caaaatcatt
    39781 tgtttctttt tatggatttt tccttgtcaa ttcgttgatt tcgcgctaaa ttaaaagaaa
    39841 gcgaggcgtt tgaatggaaa aaaaaggaaa aatcaagaaa ttagataaga ctcagatata
    39901 ttttattcta aaaggaatat ttgttggact gctgacagga atcgtcgtta gcctgtttcg
    39961 attatctatc gaaaagctaa gcgatttagt tcgaagtatc tatgaaatga gtcgggaaca
    40021 gccgatttac ttaattggcc tagctatttg ctgcatcctt gcagcatttt tcgtgggtta
    40081 tctggtcaaa aatgaaccgg atatcaaggg aagcgggatt ccacaagtag tgggacagct
    40141 tcgcggggaa ttatcaatga actggttctc tgttttatgg aaaaagttca ttggcggtgt
    40201 actttcagtt ggtgcgggcc tttttctagg tagagaagga ccttctatac agcttggcgc
    40261 atccattgga caaggagctg gacaattgtt tcggagtcca tcttcagaag aaaagatttt
    40321 aatttctagc ggtgcaagta gcggacttgc agcagcattc aatgctccta tcgcaggatt
    40381 actctttgtt ttagaagaaa tccatcactc cttttctcca cttgtttggc tgacatcatt
    40441 tgcttcagca atcactgcta acttcgtctc tctttacttt ttcggcttga gacctgtttt
    40501 atatctaggt gaccttcctt ctttgccgtt gaaatattat ggttccttag tgatcttagg
    40561 agtacttcta ggcatcttag gttttattta tcagaatgtc ttgctggctt tgccaaaatt
    40621 ttacaaccga ttgcctttac cagcttattt tcacgggttc gttcctttct tgctgatttt
    40681 gccaattggt tatttatggc ctagtactct aggcggaggc aatcaaatcg ttttagcttt
    40741 tggacatgct tccttgccaa tcattatgat tatcggtttg tttgttcttc gattcgtttt
    40801 ttccatgatt tcttacggtg ggggtcttcc aggggggatt ttccttccga tattgacatt
    40861 gggggcttta atcggtagct tttatggaaa cattatggtt gatttcggga tggatccaat
    40921 ctacatcaag gattttgtcg ttattgccat ggctggttat ttcacagcga ttggaaaagc
    40981 accgctgaca gcaattattt tagtgacaga aatggttggc tctcttaatc atctgatgcc
    41041 tttaggattg gctgctcttg ttgcttatat cgtcaatgac cttctcggcg gtaatccaat
    41101 ttacgaatca ttattggaac gtctacttgg aaatactcag accgcctcac taaaaggtgc
    41161 aaggacaact tttgattttc cagtaacggc agagagttct ttagacggat taatggtacg
    41221 agacttcaat tggcctaaaa atatgctgct aatctctata cgcagaggaa atcaggaaat
    41281 cttgactcac ggtgatacga tcatgaatgt aggagatatt ttggttatct tgactgatga
    41341 agcaaacttg ccaagaatca agcaggaaat cgaggctaaa tcttctatca caaatttgaa
    41401 gcaatcattt taacgaacat ggaaaaa
//
LOCUS       DACEKH010000013        41253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEKH010000013 DACEKH010000000
VERSION     DACEKH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.013"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            161..445
                     /locus_tag="IW662_000627"
     CDS             161..445
                     /locus_tag="IW662_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ0865476.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(726..1145)
                     /locus_tag="IW662_000628"
     CDS             complement(726..1145)
                     /locus_tag="IW662_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ0865477.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1147..1731)
                     /locus_tag="IW662_000629"
     CDS             complement(1147..1731)
                     /locus_tag="IW662_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ0865478.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1778..3253)
                     /locus_tag="IW662_000630"
     CDS             complement(1778..3253)
                     /locus_tag="IW662_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ0865479.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3434..3745
                     /locus_tag="IW662_000631"
     CDS             3434..3745
                     /locus_tag="IW662_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865480.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3860..6196)
                     /locus_tag="IW662_000632"
     CDS             complement(3860..6196)
                     /locus_tag="IW662_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0865481.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6338..7006
                     /locus_tag="IW662_000633"
     CDS             6338..7006
                     /locus_tag="IW662_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0865482.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7009..8052
                     /locus_tag="IW662_000634"
     CDS             7009..8052
                     /locus_tag="IW662_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0865483.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8289..9170)
                     /locus_tag="IW662_000635"
     CDS             complement(8289..9170)
                     /locus_tag="IW662_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0865484.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9329..10057
                     /locus_tag="IW662_000636"
     CDS             9329..10057
                     /locus_tag="IW662_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ0865485.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10213..10530
                     /locus_tag="IW662_000637"
     CDS             10213..10530
                     /locus_tag="IW662_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0865486.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10523..11131
                     /locus_tag="IW662_000638"
     CDS             10523..11131
                     /locus_tag="IW662_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ0865487.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11186..11977
                     /locus_tag="IW662_000639"
     CDS             11186..11977
                     /locus_tag="IW662_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ0865488.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12115..12603
                     /locus_tag="IW662_000640"
     CDS             12115..12603
                     /locus_tag="IW662_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0865489.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12654..13526
                     /locus_tag="IW662_000641"
     CDS             12654..13526
                     /locus_tag="IW662_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ0865490.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13702..14064
                     /locus_tag="IW662_000642"
     CDS             13702..14064
                     /locus_tag="IW662_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0865491.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14073..14825
                     /locus_tag="IW662_000643"
     CDS             14073..14825
                     /locus_tag="IW662_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865492.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14839..16641
                     /locus_tag="IW662_000644"
     CDS             14839..16641
                     /locus_tag="IW662_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0865493.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16918..17959
                     /locus_tag="IW662_000645"
                     /pseudo
     CDS             16918..17959
                     /locus_tag="IW662_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(18011..20155)
                     /locus_tag="IW662_000646"
     CDS             complement(18011..20155)
                     /locus_tag="IW662_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0865494.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20512..21885)
                     /locus_tag="IW662_000647"
     CDS             complement(20512..21885)
                     /locus_tag="IW662_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0865495.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21885..22280)
                     /gene="rbsD"
                     /locus_tag="IW662_000648"
     CDS             complement(21885..22280)
                     /gene="rbsD"
                     /locus_tag="IW662_000648"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ0865496.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22308..23225)
                     /gene="rbsK"
                     /locus_tag="IW662_000649"
     CDS             complement(22308..23225)
                     /gene="rbsK"
                     /locus_tag="IW662_000649"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ0865497.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23333..24346)
                     /locus_tag="IW662_000650"
     CDS             complement(23333..24346)
                     /locus_tag="IW662_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0865498.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24427..25026)
                     /locus_tag="IW662_000651"
     CDS             complement(24427..25026)
                     /locus_tag="IW662_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0865499.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25212..25913
                     /locus_tag="IW662_000652"
     CDS             25212..25913
                     /locus_tag="IW662_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865500.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25923..28634
                     /locus_tag="IW662_000653"
     CDS             25923..28634
                     /locus_tag="IW662_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0865501.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPETGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29085..30713
                     /gene="treC"
                     /locus_tag="IW662_000654"
     CDS             29085..30713
                     /gene="treC"
                     /locus_tag="IW662_000654"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ0865502.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30773..31954)
                     /locus_tag="IW662_000655"
     CDS             complement(30773..31954)
                     /locus_tag="IW662_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0865503.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32206..33729
                     /locus_tag="IW662_000656"
     CDS             32206..33729
                     /locus_tag="IW662_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0865504.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33798..34526)
                     /locus_tag="IW662_000657"
     CDS             complement(33798..34526)
                     /locus_tag="IW662_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0865505.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34539..35820)
                     /locus_tag="IW662_000658"
                     /pseudo
     CDS             complement(34539..35820)
                     /locus_tag="IW662_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            complement(35961..36563)
                     /locus_tag="IW662_000659"
     CDS             complement(35961..36563)
                     /locus_tag="IW662_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ0865506.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36808..37014
                     /locus_tag="IW662_000660"
     CDS             36808..37014
                     /locus_tag="IW662_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865507.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37326..38045
                     /locus_tag="IW662_000661"
     CDS             37326..38045
                     /locus_tag="IW662_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0865508.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38126..38809)
                     /locus_tag="IW662_000662"
     CDS             complement(38126..38809)
                     /locus_tag="IW662_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ0865509.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39027..41213
                     /locus_tag="IW662_000663"
     CDS             39027..41213
                     /locus_tag="IW662_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0865510.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        13388 a         7315 c         8270 g        12280 t
ORIGIN      
        1 aaaaacagct tctcatgttt gttgactatt gtgacttgtg accgaggagt tggcaggaga
       61 acaccgttta ttcgaaaaaa ggaactgcga caagactttt gtcacagttc cttctttttt
      121 ttgctatgct atactagatg taaaaaatag gagaaataaa atgagggaaa tttattcttt
      181 tgaatcaaaa atctatgctt ccgaagtcgg caagggcggg gcatatatta tttttccgta
      241 tgatataaga aaagaattcg gtgctggaag agttaaagtc caagcaactt ttgacaatgt
      301 gccatatgaa ggcagtatcg tgaatatggg cgtgaaaaat ccagatggtt cagtctgcta
      361 catccttgga ctacgaaaag atattcgtaa aaacatcgga aaagaaatcg gtgatattgt
      421 agcagttaca gtgaagcaaa aataaaagag gttgtgacaa aagacgtgtc acaacctctt
      481 gataaacggt gttcaaaagc cagtttctcg gtagtaagtc aaatttatgc aaaaatgtgg
      541 aaacccatgt ttacaaaatt tttcttacta ctcgaaacga agcggcttta tcacaacctc
      601 ttgataaacg gcgttcaatc agctgtccct cggtattagc tcaaaaacgg caaaatatga
      661 gaagctattt ttccattttt ttctctcata ctcaggtcat aacgctgatt tcacaacctc
      721 ttctttcata atggtttttc accatcttta taataaactg gttctttgat atggaaatct
      781 attttttcca accaatctaa aatcttgtaa cctaactctt ctgacatcgg aaaagaggcg
      841 ttgatttgtg cattggagat atggtcaaca tggataccgg aagtgatcgt acaacttccc
      901 ggaacggcag ttttgatgat cacagctatt ttttcggcta agacatcgtc tttatggaac
      961 cgaccatcgt ggctagggaa acgtacagtt tgcatctctg tatcacgaga aaatgtagtg
     1021 actgttccga tatgtggtgt atctccgcct gttaaagtga tcaataagtc tttaccgatt
     1081 actttaacag cagcttggat cgtgaaacct agtttggtta cttcaactat cgtttctttt
     1141 tccatattaa tcaccctgcc aacgttttga atgctcattt acaatttcaa attgatccaa
     1201 aagtttcatt gtatggtgat taacaagatc ttggatcgtt tgaggctgat tataaaaagc
     1261 aggaattggt ggtatgattt gcacacctag tcgtgctaat ttagtcaagt tttccaagtg
     1321 aatggcattt aatggcgttt cacgaggaac aatgactaac tttcgttgct ccttcattgt
     1381 tacatcagca gcacgtgcaa tcaggttgtc gcctaaacca acagagatac ctgcgactgt
     1441 tttcatactt gccggtacga tgatcattcc atcggtcaaa aatgatccgc tggcgacagc
     1501 agcacctaaa tctttgtttg aataatggta atcaaacagg ctttcgaatt cagttagtga
     1561 taagtcggat tccagcttta agttctgttt tgcccatgga ctgatgattc cgtgtgtttc
     1621 tatcatcgga tattctttta gtttcttcac taggttgatg gcataaatgg tgcctgaagc
     1681 gccacttaca ccaatcacga tttttttctt ccgttgtgcc aattgttcca ttttccgcac
     1741 tcctttttat gatgtcaaaa cagttgcaga tcattcatta tttcaaatat ttttcccaat
     1801 cttctacttc ttggaatttt gcacgtacaa aatgttcttt catatcgaaa ggaactgtac
     1861 cgtcaaagat agtttttgca cttatcccac ggaaacgaat agagccagga ttatattctg
     1921 gtaattctga tgggtccaat ggatggttcc tcattcctgg gatcacgatg atatctttat
     1981 ctccttggaa acgagtgttg aaagtccaaa ctacatcgtt catgtcgaaa atatcaacat
     2041 cttcatcgac taaaatgact gtcttcaatt ctttgaaggc tgataaagca agaatagctg
     2101 cttgacgctg aataccttca tccgcttcgt tttctttacg gatttgcatg atggtcatca
     2161 atttaccacc acctgcagga ggattgtaga catttactac ttttcctgga atagctttat
     2221 cgaccaaggc aagaatactt gcttctgtag gaataccggc catagatacg tgttcttcac
     2281 tgggaccaat cgttgtttgc atgattggat tgtcttttcg gtgagtaact gctttgactt
     2341 tcactacttg tacagctgga ttagcatccc cagtatagcc aggaaattct ggcattgctt
     2401 ttcctgagtt cgtgttgata tcttcttgca ttgtttcatt tggcatgatt tctgcttcaa
     2461 tgatgaattc agcacgagca atacccactt catcgactgc tataccatca acgatttgaa
     2521 taggttcgtt acgcaaagct cctgcacacc atagttcatt gtagccaagt ggagtggtag
     2581 gtggttcgaa agtcgtgcca atggcaattg ctggatctaa accaatattg atagtgatag
     2641 gcattggttt attcaaggct tcgaattgtt tttggaagtg accaatgtgg cgtccgccag
     2701 gcatgatgta cataccgatc gtatctttat cttctagaac catacgatgg atcgttacat
     2761 cgctcattgt tttttctgga tctgatccta agactacacc cattgtgatg tatggaccag
     2821 catcttcagg agtatttgta ggtgcaggca aaatgttgcg aatatcgaag tctgcatcgg
     2881 tagctagatg aacgacttct tgtgctggag cgtctgcttt gtctactttc acaggtttga
     2941 ttggattttc tacagcgtct ttcaagaaac gaccaagtgt tcgataatca tggtgcaaaa
     3001 tttcacctac acgtttacga ctagccattg ttccgatgtt cacacgtgtg ccggggaaac
     3061 ctttgatatt gttgaaagtc atagttggtc cttcttgagt cggacgtttt actgtaccgc
     3121 cagcaccgat ataacggtaa acaccagata attctgcttc aggatcgatt tcaatatctg
     3181 tttcgtgata ttgacctgga agtgttttta gttcttcgat gacttttctt aaatcatacg
     3241 gttgatctgt catgagagat tcctccttag ttgtctttac ctatctatag taaaaggtga
     3301 aaaagaggaa aacaattcaa cttgttcggt aaagtattct acaatggaat ggatgcgttt
     3361 ttttgataat agaataaact atgatatact tatcactaaa agaaagcgca tacttagtgt
     3421 tggaggaagg aaagtgaaaa aaatggagga gtcatttcct aaagcagtaa aagtggaaaa
     3481 tatagcgaat attttgaaag ttacatttga aaacggagaa gtaaaatacg tgaaatctca
     3541 ttggacagaa gagataaccg atgccttgca atttggaaaa aaaggaagag gaaaaagaaa
     3601 aaatcttctc gcactttctc gtaatatgtg gattgggact gaagtaacga ttgaagcaga
     3661 tggaacagta ttcataaatg gaaaagacag gtatacgcca gaagagttgt ggtacaaagg
     3721 aaagaaaagt ataccagaat tataaaaaat aattaaaaaa aacagcgcga aacaaggaac
     3781 atattacttc ctcgtttctt gctgttttct actatttcag cgctcttatg actcaaatct
     3841 aaatgtggtt gtcacaacct tattctgcac gcaaagcgat agcggcgtct tttttcgctg
     3901 ccattcgtgc aggaagatga ccgccaagca tcgtcaatat agtagagacg atcactagaa
     3961 taatcgcatg gacaggattt agttgagcaa cattttttag atccgtcatg ttgtataaga
     4021 tgctattgat agggaacgta gctagccatg caataaacac acctaatatc ccagaagaaa
     4081 tacctaaaat acacgtttct gcgtcaaata cgcgagtgat gtctttttta cgtgcaccta
     4141 aagcttttag tacaccgatt tcttttgtcc gttcaatcac agatgtataa gtaatgatac
     4201 tgatcatgat catacttgtt actaaagaga tccctgcaaa agcaataagg acataagtga
     4261 tagcatccat caatccgcca gtcagttcag tcatcgttcc agctaaatca gtataaatga
     4321 ttttatcttc atctgatttg cctttattgt aatcatctag ataatccaag atcttttctt
     4381 tgtcttcaaa attgttcgga taaatcatga tacttgaagg caagctgtct ccacctaaat
     4441 aactaagcaa tgtttgtttg gcattttcgt caactttttc agtagttaag acatttacac
     4501 cactgtctct ttgagctttg acgatttctg attctttgtt ttcgttgacg atttgagtcg
     4561 ttaattgatc gctataagcg attcctggag ataagagatt cattgtagaa gaagatttca
     4621 cgcgtaggat ccccgagatc ttcaattcat cagaagcatt ctgatacatc gcatcataat
     4681 ctgtattagg gataaaattc ccagttggaa gttttgtata gtaagtattg ttgtttacta
     4741 atttgaatgt tgtaccaacg atatcatcaa aatcaagttt ttcgtcttct ttcacatcaa
     4801 atccaagatt cttcaatgcg ttgatattag tattattatt gccgtctacg atcagcacga
     4861 catctgttgc tgaggctgga taacttccgg caagtaaaga ataattgtct tttaaaaagt
     4921 tttctttgct agtgtcaagc tgtgtaggga aacttgacac tcccacacct gtcattgctg
     4981 acatcgtact agataaagaa agagattccg tatcaggatt ttgattcgag aagctgacag
     5041 gctgtacttt gccatttaca tcacgtaaaa gattgattcc tgtagtacgg gtaaatccga
     5101 tattattact taagttagga tcgatgtctg ttacgtaatc gacgtatgtt tgatcaatct
     5161 tattcgtgtg ctgtgcttta tcttcttcgc tgacttttgc agtaatcgtt ttgctgtctg
     5221 gataatccgc tttgcttgca cccaatccgg catcgtcacg agtctggtta gtcgttacct
     5281 tagaaatagt aatcggaaat ttcgccatcg tttcagcttg cgtgttatcg atttgttttt
     5341 ggaatccaga agaaagggac agaacgatcg cgattccgat gatcccaata ctggaagcaa
     5401 aggaggtcaa gaaagtacgt cctttttttg ttcggatatt gttgaaggaa agctttaacg
     5461 ctgtccaaaa actcatcttt gttctgcgta agttaaattg atcatctttt ggtcgttcga
     5521 tgtgaggatg ggaatctgtt aagatcttac catcagaaaa ttcaatgatt cgatctgcgt
     5581 attgatgggc cagttcagga ttatgtgtga ccatgatcac taatttttct ttcgataact
     5641 cttggatcaa ttccatgatc tggatacttg tttctgtatc taacgctccg gttggttcat
     5701 cgcacaaaag gatatctggg tcattggcta gtgctcgagc gatggccact cgctgcattt
     5761 gtccaccaga taactgattt ggctttttat gcatatgatt tgtcaagccg actcggtgca
     5821 atgcatcttc tgcccgtttt cgtttttcgt ctttagaaac accacttaat gtcatcccaa
     5881 gttcaacgtt ttcaataatg cctagatgac caatcaagtt gtaactttga aaaatgaaac
     5941 cgatagagtt gtttcggtag gcatcccagt cactgtcctt aaagtcttta gtagactttc
     6001 cgttgattac catatcaccg gaatcataat tatctagtcc accaatcaca ttcagcatag
     6061 tcgttttacc agaaccacta ggacctaaaa tcgctacgaa ttctttttgt ctgaaagcga
     6121 cagacacacc atccaatgct ttggtggtgg tttcgccgac tttataatat ttttttatat
     6181 cttttaattg aagcataatc acccttctcc cgtattgaaa tattcggtat actaataagt
     6241 atatcagaac aatgatcgta gtatacgaaa taaatgttaa ctgaagatga actacaagaa
     6301 aaaatctaga aatttttcaa aaaacagagg gagatcaatg aatcgtattt tagtagtaga
     6361 agatgatccg caattacagt tgctttaccg ttctgtgttg gaacgagcgg gcttttcagt
     6421 catcaccgca ttgaatggag aggatgcgct gaaaaaacta gaagcgacac gtatagaatt
     6481 aatcattaca gatatcatga tgccgaaaat ggatggctat gacttgctag actcattacg
     6541 tagcaccaga tttgacacac ctgttctaat gattacggca aaggccgatt ttgaggataa
     6601 gaaacgcggg ttcaaattag gagcagatga ttatatgaca aaacccatcg atgtcaatga
     6661 gatgatccta cgtgtggaag cattgcttcg acgttcaaag atcaattaca gcagtagctt
     6721 gataatcgga caaacaaaat tagagctaga tgcttataca gtgttacaag atcagcattc
     6781 gttgattctg cctcaaaaag aatttctgct tctgtataaa ttactgtctt atcccaacaa
     6841 gatcttcact cgccaacagt tgatggatga tatttgggga ttagatacga atacagaaga
     6901 acgaacgatc gatgtacata tcaaacgttt aaggacccgt tttgaaaaga cggaagattt
     6961 tcaaatcatc acagtacggg gactaggcta taaggcggtt atttaaagat gaaaaaaaag
     7021 ctattttcac aattatggat ctattttgct agtattgtct tcatttcgat cttagtgaca
     7081 ctgctgtgtt ttctcggttt tattttctta ttgtctcgac attctatctc gcctgctgaa
     7141 tcggctggaa aaccaacgtt atacccgtta ttcgtctttg cggggttcag tatggtcgtg
     7201 ggtacaggaa tatcgatttt tgttgggcgg cgtatcctgc atccaatcag tgcgctaggg
     7261 acaaatatga gtctggtagc aacgggagat ttttcgattc gaatggatga acagcagaaa
     7321 gtagcagaag tacagcaact gtataaagat ttcaacgtta tggtccaaga actcaatagt
     7381 atcgagacac tgaggaatga ctttgtgtcc agtgtatccc atgaattcaa aacaccacta
     7441 gcaacaatcc aaggatatgt tcaattgtta caagccccaa atctctctga cgaagaacgc
     7501 caaatatttt tatatcggat tatcgaaagt atcacgcagc tttcacaatt aacagagaac
     7561 atcttaaaat tgaacaaact ggaaaaccag cgtatccaac tggaaaagaa agagtatcga
     7621 ttagatgaac aaatcagaga agtaatcgtt ttttttcagc ctaaatggga gaaagaacag
     7681 ctagaattgg atatcgaact tgctgcagta aattacacag gcaatgaaga gtttttgtac
     7741 caagtgtggc tgaatatcat ggataacgcc ataaagtaca atcaaatcaa tggacagatt
     7801 catatcaaac tatttgagac agccacagag attgtactgg aagtgaccga ttcaggtgta
     7861 ggaatgaatg aagagaccag agatcgaatg tttgaaaaat tttatcaagg agatacaagt
     7921 cgtcaaattt ctgggaatgg gctaggactt tcactggtta aaaaaattct agaactgcat
     7981 gatggacgta ttgattatag cagtatcgaa ggagtaggaa cgacagcaat gatacggtta
     8041 agtaaacaat aggaaaaaga ataaaacccc taaaaattct attgaaggtt tttaggggtt
     8101 tttagtcgtt tttttgtgat tagggaaatg ttcttacggt tcttttggca taaaaatcgc
     8161 tgctttctta ttgaactacg agaaatgtta gaggtgtcat tgaaacaagg acatgatgca
     8221 agaaagagag cgaaagtcat gcgtcgctct ctttttttgg gtggatagat ggacagaaaa
     8281 tgtagggctt agttttcttc ttgatttaaa tactcccaaa atgtatctga agccaaagaa
     8341 tgctcaccga ctttacgagt aaatgcaaga taactctctg ttgttggaga aatagggaga
     8401 atcacagtat tttcttttaa tagattcttg actgtttttt ccataacaat agcaatcccc
     8461 atgtcatttg caatcatatt gatgatcaag tcgattcttg ctcctttata agagattttt
     8521 ggttcaaatc cagcttcgcg acacagatct agtacaggtt gatacaagtt cgtagtgggg
     8581 ccaaggagca aaaagttctc ctttttcagt tcgcttagat ccaatttctt tttactagct
     8641 aaaggatgat ttttaggtag aactgctaca aaatcgtctg tttcagtagg caaccactca
     8701 atcgaacttg gtggggcctg gaaacttcta gcaaagtgga tcgtgtcact atgttcattg
     8761 gcgtcattgt aagttaattc gacactttcc acttctttca actggactgt aaattcagga
     8821 tggcgtttca aaaactcggt gattttggaa aaacctttat aattgatcat tgtcggtatc
     8881 gttaagatat ttaatgaaag attgttgtct ttcgtatagg tttcaagcac ttcttgcata
     8941 gcatggtatt gttctacgac attttttaca tatgggagca ataagcttcc agcttcggtc
     9001 aattcgattt ttctattggc gcgttcgaat aatttgacgt ctaattctgt ttctaacgaa
     9061 agaatctgtt tagaaatatt tccttgggtg gtgaattgtc gatcagcagt tttggtgaaa
     9121 ttcattgttt cggctaaatc aagaaaaact tcgaatttat ggatatccat catattcctc
     9181 ctacttattc caataaagaa tactttaaca caaaaaatga attggtgtaa tcttctctgt
     9241 ctgttacaat ctttcttgca gcgaaagcaa taattacaga aatttttctg atttttttcg
     9301 cttcgtagaa aaagagagga gttttttgat gccaactgga gttttgatca atgtaggttc
     9361 cgttttgttt ggcggactaa ttggcggatt agtaggaaat aaattaagtg aacattttaa
     9421 agcacagctg acgatggttt ttggtgtgtg ttcgatgggg atgggaatct attccattgc
     9481 gccgatgaaa aatatgccgg ctgttatttt tgctttagtg ataggaacag cgattggttt
     9541 gattgttcat ttaggtaatg ggataaataa gggggctgcg ctgatgcaag tacctatttc
     9601 taaaatcttc cctagtgaaa agcttggtat gacccacgat gaatttatca gcacattagt
     9661 gacggtcatt gttctatttt gtgcaagtgg tacggggatc tatggaagtt tagattctgg
     9721 gatgacagga gacagtacga ttttgatttc taaatctgtc cttgattttt tcacagcagc
     9781 tatttttgcg tgtaatttag ggtatgtcgt ttctgtgata gcaattcccc aattcattat
     9841 tttttatatt ttgttcttat tggcaaaatt tatttatcca ttgactacac cagacatgat
     9901 cttagatttt aaagcttgcg gcggattctt gatggtcgct accggtttta gaatgatcaa
     9961 cgtgaaaatg ttcccagtag ctgacatgat acctgcaatg atcgttatca tgccactgag
    10021 ctggatgtgg acaaattgga ttatgccact gttataataa aaatcaaaga gaagccattt
    10081 tttgctgact aaaaaggatc aaatctttta gtcagttttt ttgtatttga caaactgatt
    10141 ttgaaactat attatatgtt gtataatata gattgaaata aaatattaca cttgagaata
    10201 gaaggtgacg taatgcacat ccagattcca actgttcttc ttgatggtac tgtgctagca
    10261 gcattaagca aggaagatat gtatgggtat gccttaacaa aaatcgtaca agaaagtctc
    10321 tctgtcagtg aatctacact atatccggtt ttgagaagat tgaaaaaaga aggatatctt
    10381 gagacctatg atgaaccttt tgaaggcaga aaccgtcgat attactcgtt gacaccaatg
    10441 ggaagtcgtc atttagaaga aattattgat gaatggattg tttttcgaga ttctttagac
    10501 cggttattgg aggagagcac aaatgaatga atatttgaat gaagttgtgg atcatcttca
    10561 agaaattcct gaagacgatc gttttgattt gatccagtat tatgaggaat attttttaga
    10621 ttctggaaaa acattagatc agatcattga tgaatatggt acaccaaagc agtttgcctt
    10681 aaaattaaaa atcagttact tttcagaaga agaacaagca gaaaacgagt tagtgaaaaa
    10741 tccaaaaaga caaattcgcc tgatttggtt aatcattatc ggtttatttg cttctcctgt
    10801 cctcattcca ctagcactgg ggtttatatt ggttattggg ggactattgg ttgctttact
    10861 agcgctcatt tttagtatct atgttttatg tatcagtatc ttaggtgttg gcctattcat
    10921 gatcatctca ggaatcagtg tgtttggaca atcgattctg agtggattat tctttatcgg
    10981 tgtcggtctg ttcgccacag gaagcgcagt atttatttcg ccactgttgc tgaaaggaac
    11041 aaaagggctt ttttatgtca tgatgaactt tgttaaatgg atgggacgac gttttattac
    11101 aaaaagagat ctgcattcag cgggcgtttg aaggaggaca cataaattga gattgaaaca
    11161 ttttttgatt accggtattg gtctgatgct tactggtggc ctcctagcag gtgcaactta
    11221 tacattaggc gctcaaaaga gtctgacttg ggataatggt cctcggttga tcgatatggt
    11281 tactgagaca aaaaaaatca aggatgcaaa caaaatcgtc atccatgcag aaaatcaaac
    11341 agtttcggtc atacgcggat tggattttga gataaaaacg acttatgata agacagaaaa
    11401 gccagaaata aaggagaaaa atggcgtgtt gactgtgaat gcagtcaaaa aatcaacagg
    11461 tgcaatcatt agtttagaag acacaagtgc acaaatcact attgttgtcc ctgcagatca
    11521 tgaattatct gaattgacaa tcgaagggaa taatagtgca attgatatca acgaagtatc
    11581 aatcaaaaaa gtgaatacta agacacatga tggctgggta tcgttcaatg aatttacagg
    11641 aggaacaata actgcgaaag atgaattagg ctcacttcga ttagatgagg ccaatgtgga
    11701 taacttgatc gtcacaggtg aaaacagcaa cttccatcta gaagacagtt tgatcagcaa
    11761 agaaggaaaa gtcatcctaa acggtggcaa tttatctttg catgagatgg aaaacagtgg
    11821 ctatactatc caaacgtctg gtaaatctta tattgaaaaa gactaccagc caataacaaa
    11881 tactttgtta gaacaaggac agaaaaagct aaagatcact gctaaagaca catcgatcaa
    11941 tattacgacg gatcaagagg acaatgaaac agattgatcc tgtactcaac tggatatttt
    12001 aagaaaaaac agcaaagatc cttccaatct ttgctgtttt tcatcgctta acgcacataa
    12061 ctatagtaaa ataagaaaga aaatatctaa aaagaaaaca gggtgaaaga gtatatgaag
    12121 gtagaagaag gtcaaagaga aaagttaaaa acagaaatgg aaagattaca tacttatatc
    12181 actcaactaa gccaaacctt ctatgatcca gacaaagaaa aagtgatggt taattacccg
    12241 aacaattcag aaggtcgcca attagagcaa gtgtaccatg aagttttcaa acatttattg
    12301 acggtgaaga aagagctgga ttattattct ttaccgatta tcgatacagg aatattgaag
    12361 tatgatggga agaaggaacg atttatcttt aaatctgttc gggaagattt gcctctttca
    12421 gcagggatgg atctagaggt cttggtagaa gactatttta cagaagaaaa gcactgggtt
    12481 cgaacaaaat tggattattt accgcaggca gccggaggga cacaggcaag cggttggtac
    12541 atcactgaag acaaagagtt agaactagaa ggtgtaatgg cgcgtattcg aaaaaaaaat
    12601 taaaaaagta acattagata tttattgaag gataaaggag cgcaggaagt agcatgagag
    12661 aaaaaagagc attttatatc aatggttatg cagggattgt cggattggtc gtattagctt
    12721 tagttggatt gtttttcttc tatttaggta tgtggcaagc gaaagtttgg gcactttttt
    12781 taagtatttt tctttggttg atcacgttat tgttgttaag ttcggcaact gtagtcagtc
    12841 ctaatcaagc aaaagtaatt ttgttttttg gacaatattt aggtacgatt cgagaaaatg
    12901 gctttttcct tacgatacct ttggcacaaa aaatgactgt ttcgttgaaa gttcggaatt
    12961 tcaatagttc tgttttaaaa gtcaatgatt tggatggaaa ccccatcgaa atctcagcag
    13021 tagtagtctt taaagtcgtt gatacagcaa aggcattatt cgatgtagcg tattatcaag
    13081 attttgtaga aatccaaagt gagacagcca ttcggcatat cgctagccaa tacccgtatg
    13141 atacattcaa tgagaatgat ttgacgcttc gaggaaacac gacggcagtg tctgacgagt
    13201 tgcaaaaaga gctgcaagag cgtttagcag tagcaggtgt cgaagtaatc gaaacacgct
    13261 tgaaccattt agcctatgca acagaaatcg ctagtgccat gctccaacgt caacaagcaa
    13321 aagccatttt gtctgctcga caaaccattg ttgaaggagc tgtcacaatc actcaaatgg
    13381 ccttagagca aatcgaagat ggacaagaaa tcaatttcac cgatgatcgg aaagtccaat
    13441 tgatcaataa tttacttgtc tcaatcatta cagacaaagg aacgcaacct gttatcaata
    13501 caggcgacat gaaagaccgt aagtgacata ctttttgtaa gataggaaga aggagtttag
    13561 gacggattat tgtcttgaac tcttttttta gttgttttaa agaaaatgac aaaattgaaa
    13621 gaagaatgat agttgattcg aaggcagaat tgttcgcttt tttctataat cagtatataa
    13681 agtaagagag aaggggaaaa agtgaaaaaa cttctgatcg gtctacttgt tttcatcgtt
    13741 gtggtacttg caggggtgaa aatcgcagat tacgtagtta tgggtggaga agaatactat
    13801 gtacaaatta cgacagatgg tacaaaaaca gtttcaaaat cagactctgg acaagaaaca
    13861 gtcaactata gatatatcct gcctggctat acgaaagagg gtaaggaaaa agaactggac
    13921 ttcaagggac aattgactcg accgcttcgt aaaggagcat atttaaaaat cacctggaac
    13981 aaaaataaag gtgtcacttc gtatgaagaa gtcgaaaaaa aagacgtgcc gaaaacagca
    14041 ctggaaaaac taattaaagg gtagggaaag aaatgaacaa aatagtcgaa gtacagaatg
    14101 taacaaaagt atacggaaaa aacaatgaga aaaagacgca agcattaagc ggcatcagct
    14161 tcgatgtaga aaaaggcgaa tttattggga tcatgggcgc ttctggttct ggtaagtcga
    14221 cacttttgaa tattttatcc acattagata aaccaacaga tgggcatatc cgaatcaatc
    14281 aaaaagatgt gaccacatta aaaggaaatc aactagcaga ttttcgtgcg aatgagattg
    14341 gctttatttt ccaagatttc aatttattag aaagcttgac agcacaagaa aatatcgctg
    14401 ttcctctttc tttacaaggc gtcagaccaa aagaaatcaa gcaacgtgtc caaaaaattg
    14461 cagaacgttt atccatctct catattttgg aaagctatcc ttctgagatt tccggtggac
    14521 aaaaacaacg ggtcgccgcc gcacgtgcat tgatcacaca accaacgatt ctattagggg
    14581 atgagcctac aggtgcgctg gattccaaaa gtgcccggga tcttttagat acgatggacg
    14641 aattgaatac aaaagatcat gtatcgattc ttcttgtgac gcatgatccg ttttctgcaa
    14701 gttattgcca acgtatttta ttcatcaagg atggcggtat ccatcaagaa gtgaaacgtg
    14761 gagatcaaag ccgtgattcc ttctatcgag aaatactatc tattcttgga aacttggaac
    14821 agtaggggga gattgactat gctttggaaa ttatcactta ctgggatcaa gagccgttta
    14881 agagactact tggtgttatt ctcaggatta gttatggcat ctgctatttt ttacatgttt
    14941 gaatcaatgg caagcaacga agcttttttg aaaagtaata cgatcgttgc ggctacagtt
    15001 atcattttcc gattcggttc tgttttacta gggatcatca cattcgttta tattttatat
    15061 gcaaattcct ttttgatgac gatgcggcaa aaagactatg caatgtttat gatgctggga
    15121 gcaaaaggca gaaaaatcgc acagatgatt ttttctgaaa cattcttagt agggatcgca
    15181 gctactttag tgggttcagc tgtcggtgta ggattgacaa gtatcgtcca ccgattatta
    15241 gtggatcagt tagatctcaa tatttcgcat tttacacctt ttagtattca cgggttgttg
    15301 atcactgttt tgttttttgc tgttttgttt ttattagctg ctgttgtcaa tgctttttcg
    15361 attgtcaaaa aaccgatttt agcattgatt cgtgcggatc agacaccaac aagaatcaag
    15421 cagcataaat tcttgttttt ccttgaagta gtactgggaa ttgcttttct aggctgcggt
    15481 tactatatga tgaaacacgt ggggacctat caagtctttg gtataggggt agccttagtt
    15541 acgatcgtat taggaactta tttcattttc cattctgtga tcattttctt tttatcatta
    15601 ctaaagaaat cagaacagat ttcattgaaa aaattgaaca atttcacatt gtcacagtta
    15661 aactttcgta ttcgtgaata tacacaaatg ctctcaatgg tcgctatgct gtttgccttg
    15721 gcactgggcg ctttgacggt aggattaggt tttaaaaatg aaattgcaga cttgacagaa
    15781 aacaattctg gatatgatct agtgatgaat aatcctcggg cagaagatca gcaaaaaatc
    15841 aaagaacttt caccaacact ttctgcttct tatactcaaa aagaagatgc gggaacgatt
    15901 tactataatg caagtgaatt tgcggctcaa ccattgatca tgataaaaca tgaagatgga
    15961 acaccgccga agatcacgaa agaaaaagtt cctgctaaaa aaatgaatga actgactgta
    16021 caagaggaac ttcgaagcta tgaattgccg gaacagcaag aaaaagaaat caaggtagtc
    16081 agtcaatcag aatttgatca actgaacttg ccacaatcca ctttacagct agttcaggta
    16141 aaagacttcc aagcaaacct gaaaccaatc gaaacacttg ttgctcaaaa taaaatgaac
    16201 aatccatctt tgcaaaaagt agagtacagc aaccagaaat atgaaatgta tgaagtgtat
    16261 aatggtctat tctctggttt tgaatttatg ggattcttcc taggtttagc tttcttgacc
    16321 atgcttgcta gctgtttgat gttcaagatc ttgtcaggaa gcaagagtga cattgctcgt
    16381 tatgaaatgc ttgaaaagat aggaacacga cgcgggttgc ttaaacaatc gatccgtaga
    16441 gaagtaggcg tgttattctt agcaccagga atcttaggtg tagtccatgt cttattcgga
    16501 ttacaaatgt ttggtctatt catgcaaaat ccatacaaag atatttgggt tccgtttacg
    16561 atattcttcg tattgtattt catctactat gtactgacga catggttata tactggaatc
    16621 gtattgaaaa agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaagca
    16681 aaaatagctc ctcatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga
    16741 acaccgtttg ttcggaaaaa gaggttgtga caatattttt gtcacaacct ttttttcacg
    16801 gtcttttatt agaatatagt aaattttttc tcgttactca cggaataagc tgttaagaaa
    16861 acggtttcca tgtatgctgg aatcagtgca aatgaaagaa aagagggtgc aaggaacatg
    16921 gaaatcaatg aacaaaattt tggcgaaaac tatcacttga ttacgatcac gaatcaaaat
    16981 gggacatctt tgtctgtcag tgatcttggg gcacgggttg ttcattttca aacaataatc
    17041 gataatgaaa aacgagagtt gatcttaggt tttgatgaag caaaagaata tctagaaaaa
    17101 gacccttata tcggtgcttc aatcggacgt atggctggac ggattacgaa tggcacattc
    17161 aaattagatg atgaaacgtt cgaagtaaaa acagacccgg acactggtca ttggtttaca
    17221 tggtggtgca cctagctttg aagccaagca gtggcattat acagtcatca atggtgaaaa
    17281 cgaagcatcg gtcattttct atcagactag cccagatatg gaaaacggat ttcctggaac
    17341 tttagatgta gaagtacggt atacgctgac gaacgatgat acatggcgag tgacggtcca
    17401 agggatcagt gaccgcaaaa cagtattcaa tcctacaaat catgtctatt tcaatttaac
    17461 cggtgatcca gcacagtcaa tcgacgaaca tatactttgg ttgaatagcc gattatttgc
    17521 tacgctcaat aaagatacaa cgcctacagg agaagtagtt tcagtaagtg gcacagcatt
    17581 tgacttccaa cgtcctaaaa aattaggtga ggtctttgcc agtgattttc gccaatcaat
    17641 gatagtgggc ggcatcgatc atccattttt gttgaaaaaa caatctggtt tactaaatgc
    17701 tgcggaattg acaagcccag atgaaaaagt atcgatccaa gtaaaaacag atgcttcagg
    17761 aatcgtgatt tttacagcaa attttggaga agatggaccg gaaatgcgcg ggaataaatt
    17821 agtaaatcat ggcggtatca catttgagac tcaagaactg ccaggagcgg agcgacttga
    17881 aagttttgga gatgtcactc tggaacctaa ccaagtacgc gagtttgtaa cggaatacaa
    17941 aataaatata aagaaataga ataaattaaa aagggagtgt gacagtgtca catccccttt
    18001 ttaatttatt ctatttcttt aaaggcagat caagccagaa tgatttgtct aaacttaaca
    18061 aggttagtac aggaacagtt gcagctttaa ctgtacgagt agttgtttct ggtatctgtc
    18121 ttgccgtaaa attgttccac aaatcccaga ttgaagcgac acggaagatg gaagaagcgt
    18181 tgttgacgcg ttttatcgtt gaaaattcta atcctgtgat tggcacgcca tctgttgtcc
    18241 atttgattgg cacatcgaat cgcttagaca tattgaggat ttcagcgttt gcttcttcag
    18301 ttgttgctgc ttcttttttc ggttcatcat attttgtcag gtcgtacgct tcgatgatcg
    18361 cgttcaattt ttgtgaatat tggcgatctg tagcataacg tccttccaaa tatttggttg
    18421 cttctttgta gtcttttgtt tccgacttcc aagttggttt gtagaaatca ggattgccag
    18481 tcagtccatt tttgatcaag tcggcatagt cttccaaact ttccttgtaa gaaggatatt
    18541 tacggaattg tgcgcggatt tggtagcttt gtccggaact atcttgttca agtgtattga
    18601 aggtcacgct gcttccttta tatgatcctt tgataccaaa aaggttatga tttggttcgc
    18661 ttgataataa gctgtttcca gatccacttt caaggatagc ttgagccatc atgactgatg
    18721 cgtagattcc ttctttttgc ccgatttcct gtgcatcatc tgcaatcttt ttcaagaact
    18781 gatcagttgt ttggttttga ctgaaatgga acgagccatc taatccatct aaaatagatg
    18841 agccgcctgt cagcgaacct gaagcagcag gtaaacctgc taagtttact cggacagcaa
    18901 aatcaggcgt gaaatctgaa atagaagtta ctcgaacttt atctcctggt tgaggcgcat
    18961 ggataaattg tccgttccca atatagatac ctacatggtg agtaacaccg gaagaaggtc
    19021 cccagaagac gaggtcgcca ggttgggcct gacttactgg aatctgcgta cctgcatatt
    19081 gctgttcgcc tgtccagcca ccgatataat gaccggttgc tttcatatat acgtaataag
    19141 tcaaaccaga acagtcaaaa gtatttggac cttttgctcc ccatacatat ggttttccaa
    19201 gatgcttgaa tgcttctcga acgattgctt gttggacatt cgttgtattt gtaggcaata
    19261 cagcatcaat tggtgatttt acaacaaaat cagaagaagc agaagatgga ttctctatac
    19321 cgctttgatt tgctgtattt ggacgagatg aagcattgtt cgttccgcca ttcgatgaac
    19381 cattgtttgg atggtttggt tctctctgag tatttccatt gttattcgat ccagatgggt
    19441 ttgtttgatt cgaagaattc ccgctgttcg aattattgtt attattgttt gaattatttg
    19501 aaccattatt attgttgtta ttacccacat ctggtttgtt tggcgtttcc ggatctggtg
    19561 tactagggac ttcttcacta ggatcagttg gtttctctgt tgaagtatcc ggtccagctt
    19621 taaattctgc agtaatagat aaattgttcg tacctactgt aaaggtcttt gcatcataat
    19681 ttacttcttt ggcaccatta acagcgactg aagcaatttg ttcaccttca gctggcgtaa
    19741 tagtgtaagt taccttcgtt tccgctaata aaccttctac cgttccactt tccgtaatat
    19801 ccaaaggttg ctgttcaggg gaatccgctg tcgaagcagt cagttggatc ttaccaatca
    19861 aagaaggagc aattgtaagg gaatatgtct cggctggctt ttcattttct gatggatcag
    19921 ccggatcaat ttcagttggc tcggtcgatc ctgttgttga gttagttgtt ccagttgggt
    19981 cttctgtaaa agaatccgtt gtttcactag tagctgagct ggatgtagtc gtatctgttg
    20041 tgactgtttg atcagctatt tgattatcta cggcaaatac ggtattggtc gacaaaacag
    20101 acggagcaat cagccaagta gcagctagca aacgtatcgt ttttttattt ttcataataa
    20161 actagtcctt ttcctttcat tgattttctg tcttattcag cattcattag acattttttg
    20221 gaacagataa tcgtgtcgaa aagctttttc ttttcataaa tctgtctgtg tattctaaat
    20281 aaaacaattt gtaaataagc aacgagcatt cattcgtttt tctttgacgt tcattttatt
    20341 gcatagacca cctttgtttg gggataaaca agtggggata aaaatggtta tgcatatgta
    20401 aattatacca aagtttggaa agtatcacaa aaatttataa aaataatgat cctaaaaaga
    20461 aaaagactga aatatgtgat aaacatctca tacttcagtc tttttcatta actattcata
    20521 agacattagg tcttcatcaa acccgctaac gagagtagtg atcattgcta cgactagact
    20581 aacagccagt cctgctaagt accagaaaaa agtaccggaa atgaatgcag gcaatgtggt
    20641 cagactacca aatacaaaag cgatcgcttt cacttgggca aatcccatta cagctccacc
    20701 agcagctgca ccaatgcaag cagcaacaaa tggttttttc aatgggatcg caattccgta
    20761 aacagttggt tcggtcactc ccgcaagtaa aatcgaagca aaagcagaac cggagatcga
    20821 acgggttttt gaacgtttac ttcgcagaaa tacacctaaa gctgcaccag ccattccaaa
    20881 attggctgct cctgtcaacg gaacgattcg gtcgaaccca taagcagaga tattactgat
    20941 catgacaggg tttacagccc attggatacc aaatattacc aaaatattcc aaccaccagc
    21001 gatgattgcg ccactgatcg gtccgttaat atccatcatc caagtaattg cggcagctaa
    21061 accttcgcca aggtaaacac ccgcaggccc gaataccatt aacgtcaaag gaaccgtgat
    21121 aactaatgaa agtaaaggta caaatactaa ttgcatattt tcgcgaatat attttttgag
    21181 gaatttttca acaaaagaat aaaagaaaat acctaaaatc gccggcatga cagtagacgt
    21241 atactgcatc agtactacag gaatccccag aaaatcagta gctgctttgc cactgtctga
    21301 aagtaaagag gtcagattcg gttcaatcag tgctgcagca atgacagccg caatatatcc
    21361 attcacttta aacgttttgg cagcagaaaa agcaagaaag attggcagaa aataaaagac
    21421 ggcattcgac gcagcagaca aaatatgata agttccgctt gtttcactaa gccagccaat
    21481 cgtagtcgct aaaacaacca gaccttttaa tataccagaa ccggcaaaca gcggaatgat
    21541 cggcgtaaag atttccgata tcaaaccaaa tagtttttcg atcggattta gcttttcttt
    21601 cggagctgaa tgatcgattt gacgcgtcaa ttccttgtaa gcaggagcca atccctgatc
    21661 catgattatt tctgcatatc tatcgagtaa aatgaaatac ttaatggaag ggatttgctg
    21721 ttttaatttt tctgcattgg ctactggctg ttttttcaat ttgatacgaa gccgagtcgc
    21781 acatggaagg acagaatcaa tattgtcttt tcctcccaac tgttcaatca gcatatgaat
    21841 ctgctcggac agtaccatct gttcaggatt cattgtcgtt tccattagaa gaccactcca
    21901 ctcactaata taatattggc gtaaggtgtc atttcgcctg tacgaataaa tgcatgacag
    21961 ttattcaact cttttttcat ttcttcatgt gggataaagc taatcggtgt atctggtaac
    22021 aataatttga ttttttccaa tatttccggg ttcattgtct ttatttcttc cgccaattca
    22081 atcgattgta cctcaagttc ttctaaaacg ttttctaaaa cagataaaaa acttggaaga
    22141 ccgtttgaga cagccaaatc tattttttca gtaccaaagg ggacaggcat gcctgcatcc
    22201 ccgatactta atttatcaaa gtgccccatt tgtgcgatta ctcgtgaaat atctgaattg
    22261 attacttttc cttttttcat gtttgtttct tcctctcatt gttaatttta gactgctgat
    22321 tgttctaaag cttcgcggta agggatagaa ggttgcgcac cataagactg cacagcaaca
    22381 gaagaggcta gatttccata aaggatcgct tctttcaagt tactaaggtc tggctgaagt
    22441 gtgctgctta atgcgccaat aaatgtatca cctgcggctg ttgtgtcaac ggcatccact
    22501 ttaaaagcag ggacgatacc gtgttctttt tctgtatgat agaaagcgcc ggcactgcct
    22561 agtgtaatga ttactgttcc aatacctaat tcatggagtt tttcggctgc tgcgaccaaa
    22621 ctgttatgat ctgtcactct gattcctgta atgatttctg tttctgtttc atttggtata
    22681 atgatatctg tcttttcaag tagttcagta ggaatcgttt cacgagctgg tgcaggattc
    22741 aggatcgttg tttttcctgc gtcttttgca atagaaaaag ctaggatcgt cgcatctaag
    22801 ctactttcaa actgagcaat aataaagtca ctgtttgtaa tcagttgctt gttttttagg
    22861 acttcttgtt catggaaagc attatttgcc cctgcatgga tcaagatact attttctcct
    22921 gctgcgtcta ccataatcat tgcttgtccg gttgtggcgc cttggatttc agctatccca
    22981 gatgtatcga tgttttcttt tgtcagtaga tctaataact gttgtccttc gctgtcagcc
    23041 ccaacgcctc caataaaaga tgtccgtgcg cctgaacgtt tggctgccac tgcttggttg
    23101 gcacctttac ctccgccagc gtgaaaaatc tcatgcgcat gcatcgtttc gcctggcttg
    23161 ggcatgttgg ttaaacgaag ggtagtgtcc atattaatac ttccaattac tgtaaccgtg
    23221 ttcattttac aaacctccaa ctatctcatt tattttgatg ggaaaattat tttttaaaac
    23281 gttttagtaa aacgttttaa tagttcgata aaaatatagc acaccttgaa agctatttca
    23341 agggtgcagt tgaaaatctt ttttctaatc gtacaggtaa tcttttttct tcccattctt
    23401 tttctgggaa ttggatccga tctaacaata acttggcaga tgtttgtccc aattcgaaga
    23461 ttggttgtgc gatcgttgtc agtttcggct tgatatactc gcacatatct atattatcat
    23521 agccaataat gctgtagtct tcagggatgc tttttccagc ttcctctaat ccgcgatata
    23581 atccaaaagc taattcatca tttaaagcaa acacaccagt tgcagcagaa tcaagaagtt
    23641 ctgctgtgat ctggtagcca ccttgttttg agaactgtgt aggtaagagg atcagttggt
    23701 cgtgagggat ttggtaaaca tcaagagcag atttaaaccc ctcaatccga gcgtggacat
    23761 tttccggtgg attttcagga tagacaaggg cgatcgtttg atgtcctaga ctaagcaagt
    23821 gcatgcccgc taggtaacca ccgcgaaaat cgtctgttcg tacggcatca ctaaatcctt
    23881 cagatttttt ctgatccaaa acaatgaagg gccggccttg ttttttcaaa ttttcgttga
    23941 ttgcatctgt agagacagca gaagtcgcga taatgaatcc atccacacct cgacgtgtga
    24001 gctcggctag atattcgatt tctttttgat gatcagaatc agcattacat aatatcgtga
    24061 cgaaattctg cttatacaag atatcctcaa ttcctcgcat cagtgtactg aagaatggat
    24121 tcgtgatatc aggaacgagt acaccaattg ttttcgtttc cttggtaatc atttgtctag
    24181 caaaatagtc aggctgatag cctaattctt ctttggcagc taaaactttc tgaacagttt
    24241 tcggactgaa tttctccgta tttccattta atatcaagga gacagttgtg attgaaacgc
    24301 cggaaagttt agcgacttct ttgattgtga tctttttttt caccatgttc atactcctat
    24361 ctaaatcctg tggttatttt cccatgaaat caaaaagaag taaatgaaaa tcacatgtgc
    24421 cagtacttat ttgctttgtt ggatagcttc gattatttgt ttcgttgagc gtactcgtgc
    24481 agttttcgga aatatctttt ttatgaacaa ttgatgggat tctttgtcac gatcactgca
    24541 agcatcttca gcaagcacga tatggtaccc gtgttgaaag gcatctaatg cagtagcata
    24601 tacaccgttt gttgtcgcta caccgcttaa aatgatagtt tcgattccac gccggcgaag
    24661 ttgaagatca agagaagtac caaaaaaagc acttggattg tattttgtga tagtcaacat
    24721 attatctgta tctttcaaag aatccttcaa aagtaaatca gtgaattctg gaggtacaga
    24781 agcagtcatt ccacccatat cagtttgttg actcaaataa gggaaagtgg agatgtctac
    24841 gttcactaaa actttcaagg cagcggtacg tttcaatgtt cttactaaca aatcattgtt
    24901 tgtcagaata gatgctgcag tgtgtggaga gagtgtaccg ttatttacga tctctttttg
    24961 catgtcgatc gcgacaaaag cagtttttga taaatcaaaa atcatatttt cctttaaatc
    25021 attcatgatg ttccttcttt caaataacaa tgttttttat gtaagtaaat cataaatcgt
    25081 ttatttgtca actaaattaa aggattcctt tctttagtct agagaaattc agtggtaaaa
    25141 taaataagtg tgagaaaacg cattcgatgc atagatatag cgagaaatat cgtgaaaatg
    25201 gaggaatcag agtgaagtat gtcgaagtaa aaaatgaata caaacgatac aaaatgggag
    25261 aaacgaccat tacagcaaat gacgatattt ctttttcaat tgaaaaaggc gaattagtga
    25321 ttatacttgg tccaagtgga gcaggaaagt caacgattct taatatttta ggtgggatgg
    25381 atacacctga tgaaggtcag atcattattg atgacacaga tattgcgcaa ttttcagata
    25441 aacagcttac tgcttaccga cgaacagatg tagggttcgt tttccaattc tacaatctcg
    25501 ttcctaatct aacagcaaaa gaaaatgtgg aattggcaac ggaagtttct cctgatgcgc
    25561 ttgatcctgt agaggtattg aggcaagtgg gcctagctca tcgattgaat aactttccgt
    25621 cccaattatc aggaggcgaa cagcagaggg tttcaattgc tcgtgcacta gctaaaaatc
    25681 ccaaacttct tttatgtgat gaaccgacag gcgcgctgga ttttgaaacg ggcaagcaag
    25741 tattgaaatt attgcaaaac gctagcagac aacacggcaa taccgttttg atcattacac
    25801 ataattctgc tttagcgccc atcgctgata gagtcattca cattaatgat gctaaagtaa
    25861 ggtctgttga attaaatgat catccttcat ccattgatga gattgtatgg tagggggatt
    25921 ttatgaaaac aagaacatat cttaaagcag gttttcgcga aattcggcaa tctaaaggac
    25981 gattcattgc aatcatcctt attgtcatgc taggaacact tttatttgta ggtgtcaaaa
    26041 ctgctggacc tgtcatgcaa aaaacgatgg atcactatgt tggtacagca ggactttctg
    26101 atttgcaaat cgtttctact ggtggcttga cacaaaaaga tattgctcaa gcagaaaaga
    26161 tcccagatgc aaaagtagaa acaggaaaac agatctatta ttccaatact ggcaaaaacg
    26221 aagtgattca aatattttct tataacaaaa ataacaagca gaacaagcta caattaactg
    26281 atggaaaact cccggaaaaa ccaaaccagc ttgtattgga tgaaaaagcg gaagatgaag
    26341 ggtataaaat cggtgatatt tatcagattg attcagatga attgaaagaa aaagagtata
    26401 ccatcacggg ctttgtccgt tctccattgt tcatcaacaa tttggagaga ggatacgcga
    26461 acgtcggaaa tggtagcgtc gattattttg tctatttacc tgaatccgtt tttaaaacag
    26521 atatttattc tgtcttatat cttgatttta caaacgtaaa gaatttgaat acgtacagca
    26581 aagcttacaa agataaaatg gagcaaaatc aagaaaaggc agaaaagtat ttacaagatc
    26641 gacccgaaga acgcttagaa gagctgaaaa aatcggccaa cgaagaatta gaaccggcac
    26701 agcaaaaaat agcggatggc aaagcacaaa caacgcaagc aagaacacgg ttagaagatg
    26761 ctaaaaaaca gttagcgcaa cagtcagcaa ctatccagca gcttcctgaa gcgcaacgaa
    26821 caatcgctgt tcaaacatta tctcaacaag aagctcaact tgctgaacaa gaaaggcaat
    26881 tagttgagaa agaacgtgaa ctggcctctg cagaggaaga attgaatcga aatgcagaaa
    26941 aaattgaaaa tttaactaaa ccatcatacc tatacaatga acgttcagaa aatgtcggtt
    27001 ttcaagaatt tggtgatctg tctgatcgta tcgctgcgat tgccaatgtt tttcctgttt
    27061 tctttttctt tattgccgca ttgatcacct ttacgacaat gacacgtatg gtagaagaaa
    27121 accgcaggga aatcgggaca ttaaaggcgt taggttatac gaaagttgaa atcgcaaaaa
    27181 aatatgcaat ctatgcaagt ctagcctcag ggataggtat tgttttagga acgatattag
    27241 gaacgaatct tttgccaagg atcatttttg agctgtctaa cgagcggtat gatattggga
    27301 gtgcgcttat tttttatgac tggccgccga ttttacaagc agctgccgca ttcttcattg
    27361 ccgcatttgg cgctgcaatg atcgttttat tcaaagattt aagagaacgg cctgctgcat
    27421 tgcttcaacc gaaagcaccg aagccaggga aacgcatttt tcttgaatac atcactccgc
    27481 tgtggtcacg ccttagcttt aatcaaaaaa tcagttaccg aaatctcttc cgatataagt
    27541 caagaatgct tatggcgatt atcggaattg ctggatgtgc cgggctgatg gttgcaggcg
    27601 tgggattgaa agattctcta agttctgttt cagccaaaca atttggaacg atcattgact
    27661 atcaagcaat cgttacattg gattcatcca cggaagatag tcgtcaaaaa gcagcggata
    27721 tgctgcagaa gcaagaaaaa gtaaatgaga tcctagctat tcatactcaa accgtagaat
    27781 tgagaaaaaa aggacatgct tcgcaaagtc tgacagtgat cgtaccaagt aaaacagctg
    27841 atttggatcg ttttatccat ttaactgatc ttgaagagaa cccacgctcc ttgcctgaaa
    27901 caggaatcgc tattacgcaa aaagtagcag aactatttga cttatctaca ggagatcata
    27961 tttcgcttta tgatgaagat caaaaagaat ggaaagtcga agtaaaagaa atcgtgaaaa
    28021 actatctagg tagctttatt tatatgtccc ctgaatatta tgcttcagtt actgatcaat
    28081 cgatgatgga taatgcattt ttgctgcgta cagataagat gacaacaaaa gaagaagaaa
    28141 tgctttccga gaaactgctt gccacagacg ctgtgacaaa tacgtctttc atgtccaaac
    28201 aaatcgaaac gcaagaagaa tcaatggcga acttagactc ggttgtctta atctttgtcg
    28261 tattatcagg actgctggct tttatcgtct tgtataatct aactaatatc aatatttccg
    28321 agcgtgtacg cgaattatct acaataaaag tccttggatt ttttgataaa gaagtcacga
    28381 tgtacattgt tcgagaaaat atcatcttta ctcttttagg aatcatcgga ggatttggtg
    28441 taggatatat attgactgac ttcatcttgc aacaagcgtc tatggagaat gtcatctttc
    28501 cattagtgat cacatggtgg gcttatgtct tatcagcagg attaactatc gtatttacgg
    28561 ttattgtgat gattgttacc catttcaaat taaagcatat cgatatgatt gatgcgttga
    28621 aatcgaatga ataaaaacag aggttgtgaa atcagcggtt tgacctgagt attagagaaa
    28681 caaacggcaa aatagttttt catattttgc cgttttttga gctaataccg aaggacagct
    28741 gattgaacac catgaatcag aggttgtaaa aaagccgtcc tgcatcgagc aaaaagaata
    28801 ctcaaataaa attagctcct catgttttac atttgagaag actgattgcc gtagataaaa
    28861 gcttttgaac accatttata gaagaaacaa gaatattttg ctattttaaa caagaggata
    28921 gacaaataca tgtctgatct cttgtttttt attgggcaac agacctctta gaatgtataa
    28981 agataaattt tacaaacgct ttcggttcag ttataataat aaatgataac ttgtatatat
    29041 aagttgtggt aggtaataaa tcagttgaat ggaggtaaat atccatgaca tttaaagaca
    29101 aagtcattta ccaaatctat cccaaatctt acaaagacag taatggtgat ggaattggag
    29161 atttaaaagg aatcaaagaa aaactcccgt atctgaatga tctaggcatc gacatgattt
    29221 ggctaaaccc tatttatcca agccctcaaa aagacaacgg ttatgatatc tctgattaca
    29281 cagccatcga tcctatcttt ggtacgatgg aggaattcga acagctaata gccgaagcaa
    29341 agaaataccg gatagaaatc atgctcgata tggtgctgaa tcacgtttcg acagaacatg
    29401 aatggttcaa aaaagcacta gcaggggacc agcattatca ggacttcttc attttaagag
    29461 aagagccaac ggattgggta tcaaaatttg gtggaagtgc ttggtcgcca tttggagata
    29521 cggggaaata ttatttgcac ttatatgatc ggacacaagc tgacttgaac tggcggaacc
    29581 aagcagtaag agaggaacta ttcaaagttg ttcgtttttg gatggaaaaa ggggtaaaag
    29641 gattccgttt cgatgttatc aatgttatcg gtaaagacga acaattgaaa aataataaag
    29701 aaaatgatgg caaacctgaa tatacggaca aaccaatcac ccatacgtat ctaaaagaat
    29761 tgaaccaagc aacgtttggg acggattcca ctatcatcac agtcggtgaa atgagctcta
    29821 cgacgatcga aaattgtatc ttgtatacag aacctagtag agaagaatta tcaatggtct
    29881 ttaatttcca tcatttgaaa gtcgattatg aaaatgggaa taaatggtct actcctccat
    29941 ttgattttgc agaattaaag cgactatttc atacatgggg agaaaagatg agtgagggta
    30001 acggctggaa tgctctattt tttaataatc atgatcagcc acgtgcgctc aatcgatttg
    30061 tagctatcga tcagtatcga aaacaaggag cagagatgct agcaaccatg atccatttga
    30121 accgtgggac gccctatatt tatatgggag aagaaatcgg catgatcgac ccagactatt
    30181 catcgatttc cgactatatt gacgtagaaa gtttgaatgc ttataagtta ctccttgaac
    30241 aaggattcac agaaaaagaa gcgtttcgtc ggatcaaagc aaaatctcgc gataactctc
    30301 gaacacccat gcaatggtca gacacagaac aagcaggatt tactacaggg aaaccttggt
    30361 taaaagttgg aggcaagcta gaagagatta atgtagaaaa agaactatct tctgaagatt
    30421 ctattttgtc ctattacaag aaattgattc ggctgagaaa aacatatcca atcgttgcac
    30481 aaggagatta tcatgcttat gaagcaaatc atccggaagt ttatggcttt cttcgccaat
    30541 ttgaaggaca gcagctgctt gtattaacaa atttctatga taaagataca aggatcgcca
    30601 tcccagaagg attcacagaa gccaatattc ttatcgataa ttattccgat actgttcttc
    30661 atcaaagtct ggagctaaaa ccataccagg caattgcttt gctgaaaaat taaaacaaaa
    30721 acacaaaacg tcgttcctac gagctattct cgtaagatcg acgttttgtg ttttatttaa
    30781 aataatcttc ttcagaaaat gaagagtttt catactctcc tttatcgcca taaaatttat
    30841 tgtagcgttg tttgaattta cggaaaataa aagtaacgag taatttgact aacgtataga
    30901 taggaatacc aaaaataacg cccacaattc ccattagatc acccatgaca agtaaaacga
    30961 ttaagatcgt aatcggatgg atctgtaaac gatcacccat tactcgagga atcaccaagt
    31021 caccgtggag aagttgtacg gcaaaccaga caatgacaaa tttgacagcc ataaaagtag
    31081 agtcttggaa tgcaatgatc aaacctggaa agaaggcgat aaacggccca atatatggaa
    31141 tgatacataa caacccagca gaaatagcta aaacactagc aaattctaat ccgatcagta
    31201 agaaacaaac ccagtagact gctccaagga taaatgaagc aatgatttgc ccctttagaa
    31261 atgaacccaa ttgttgatta gcaatctttg tcaaattttt aaaatctgta cgaaatgctg
    31321 gtgggacaat cgctaaaact gactgataaa atttttgagg atctctcaaa aggaaaaaag
    31381 cgacaattgg accagtaaat aaagtaataa aaatagctgt aacagttgaa aaaatgttgc
    31441 tgatccgttg tgcgccactt tcccaataat ttccaataaa tccagctata tcagacgtca
    31501 cgtcgtcaat agaagcgaaa aattgattaa atgaatcggc aaaaggtgtt ttctccaaaa
    31561 attgtcggag actctcttga aaatcttgaa acaaatctgg caaactactg atcaatgtct
    31621 ttgcctgttc ttcaagaaaa ggaataagcc atatgcttcc aagccccaat aaaaataaaa
    31681 taatcacata aacgataatg actgacaagt tcctcgaaag gtgcttgctc aaacgtttga
    31741 tgactggttc caaaagataa tacaagatca gtgcaaacaa taatgggggc aagatagtag
    31801 cgataatgat tccgacaggc ttgaatataa acgagatctg atggaaaaaa taaatgatca
    31861 gcgcaagtaa gataactatt cctaaaacat agtaagaggt ttttcctcct aaaaagcgga
    31921 tcaaacgatt cgttgtatct gtttcttttt tcactctttt tcctccttta tctataactt
    31981 cattatagac aaaagcgggt ataaaaggaa aatgatagag gttgattgca aaaatatcgt
    32041 caatcattca tataaacttt ctcatcaatt aaaataagaa aacgcttgac agttctttta
    32101 accatatgat aaattaaatc ctgttgctaa aacgtaacta ttacggtttg cgaaaagcga
    32161 atcggaacat tcaaatgaaa gaagatgaga agaggagtgt cataaatgaa acgattgata
    32221 ggtatattgg ccatgctggt tttagcaggt atgttaactg cttgcggggc aagtgggaaa
    32281 gccgaggaca gtaaagaaaa attgagcgtg atgacgacct tttatcccat gtatgatttt
    32341 acaaaagcaa tcgtcgggga tgaaggagaa gtagagttat tgattccagc agggactgat
    32401 tcccacgatt atgaaccttc tgctaaagac atggcaaaga tccaagatac agatatcttt
    32461 gtttataatg atgaaaacat ggaaacatgg gtccccgcaa tccaaaaaac attacaagaa
    32521 ggcaatgtcc atacgataaa agcgacagaa ggtatgcttt tacttccggg aagtgaagaa
    32581 ggacatgatc atgaccatga acacggggaa gaaggccata cacatgagct agacccacat
    32641 gtatggctag ctccaagttt agcaatcaag caggtagcaa acatacgtga tcagctgatt
    32701 gaagcttacc cagaaaaaca ggaagtatgg acaaaaaatg cagcagccta taccgagaaa
    32761 cttcaagcac tccatcagct ttatcaagaa acctttaagc aagcaaaaca aagatctttt
    32821 gtcacgcaac atgctgcatt caactatcta gcgttagaat atggattgaa tcaagtatcg
    32881 attgctggtc tttcttctag tgaagaaccg tctgcggcga gaattgctga actgaaacac
    32941 tttgttaaag aacatggaat caactatatt tattttgaag aaaacgccaa agatagtatt
    33001 gccagaacgt tagcaaacga agcaggtgta tctttagaag tattgaatcc tttggaagga
    33061 ttgacgaatg aacaaataga aaatggagaa aactatcttt ctatcatgga agcaaacctc
    33121 gaagcgttga aaaaaacgac agagacagaa aacccgctag aagatacttt gacccccaaa
    33181 aaagaaaaga gcgtctacaa cggttatttt gaagacgagg atgtacaaga tcgctctctt
    33241 tcagattggg caggaacttg gcaaacagta gatacgtatg tagaagatgg gacttttgat
    33301 cctgtttttg aatataaaga aaaattgaat caggataaga cagcacaaga atataaagaa
    33361 tattatacga agggctatca aacagatatt gcttcaatca agatcagtga gaaagagatg
    33421 gcatttacgt ttaagaatgg aacaacgaaa cgttcaaatt atcgttatgt agggaaagaa
    33481 atcttgacct acgaggcagg aaaccgtggc gtaaggtatc tattcgaagc agaagataag
    33541 gaaagtggcg cttttagata tattcaattc agtgatcaca cgattgcacc ggctgactcc
    33601 gaacattttc atttatatgc aggaaatgaa agtcaagaag tattgctgaa agaaatggag
    33661 aattggccaa cattttatcc agatacaatg gatggacgtg aaattgcaca ggaaatgctt
    33721 ttgcactgat aggcaaaaaa gaaccgaagg gtttggatcg gctggtcatt tagtcggtcc
    33781 aaactattcg gttcttttta tgaaatcaat ttctttaatc cacggataga ggctgggatg
    33841 gtctcgccgt ttgccaacgt gacttctctg gtttttttat taatcgaccg cacattttgt
    33901 gtattgataa cataggattt atgacaacgg ataaaattcc gatgcatttt ctcgatttct
    33961 ttgatcttct catagaattg taattgggca tgagtcaaat ggacttccag tttatgtggc
    34021 gctcctagcg aagtagaaaa atattggatg tctttgatcg gtaaaaattt gatctcatca
    34081 ttaaactcta ttttgaagat atctttttgg atttgggaca tttgaaaatc ttcgaaaatt
    34141 ttgctcagga tatgctcgac attttctttt aaatcataaa gatcttcttt gacgatataa
    34201 tcagtcggtt cgatgttgct ttttaagatt ttcatcgaca tttccgtatg ggaagtcaca
    34261 tagattattt ttcctaaagg atctaattca cggatcaatt taccgatcgc tacaccatcc
    34321 atttcactgt cttctagttc gatatctaaa aagtaaatac caaaagaaga agttctgatc
    34381 ttggctgcat tgagaagttc ttccggatca gaggtagaaa tatggacata actgtctaag
    34441 ttttcaatca tgattctatt tttgatcacc gttgataaaa cttctaattg ttttggatcg
    34501 tcttcacaaa tataaatggg aatcatatca ttactccttt attttatttg tatataaatc
    34561 gtttgagaaa aacgctggtt ttcaattgat gtttcaagga aaagataagg attggtcttt
    34621 gtcaattcat ccaagataaa caagccaagg ccttcgtttt ttggatttgt ttttgttgag
    34681 aacccttttt gtttcaactg gtttaacggt aatgtatttg ttgttgtatt gatcacacga
    34741 atcatcacgg aatgattctt cttgagtatg gtcaattcga tttttttctt ctttgcagca
    34801 ctagctcctt caatcgcatt gtccaagata ataccaagca tacgtacaag gtttaccgta
    34861 tgtttcgtat ccagttcgat tggctcagga aaatgaagct ggacgatcaa ccctttttcc
    34921 tgtgccatca tgagttttaa tgacagtaaa ctacggatct caggaacttt catattatcc
    34981 aacatactta gattggccgg aaaaaggttc atcatctggc gtgtaggtaa tatcgtttga
    35041 tcataataag accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg aagagaaaaa
    35101 agcagatttt ggtaatcatg tttgaattct ctcaagtctt gatagttttt ttctagatct
    35161 ttgatataat cctgttgcat cctgacttgt tgctctaata actgatcagc taatttctta
    35221 ttagagattt tccaataata aaccaagatg atcaggccaa cacacagata tagaatgaaa
    35281 aaactcgtat tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt ataaaaaata
    35341 atgatatagt atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg gcggtccaca
    35401 gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag ctaataaagc agataaaaaa
    35461 gcggataaga caatgtgaaa atactgcaat gaatctcctg gttcaataac cgaagatttc
    35521 aaaacaaaaa aatcgccgat gcttgttaag tgatctccta aaagcgacgt caataaccca
    35581 aaaagaaagc gcgtaaacca attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat
    35641 cagcattcct atagcaatta gagcagtaaa aatccctaaa atcgaataga cccatccaat
    35701 caaaatacaa gaaatcagaa tgactcccgc aaacagatat ttgtatcgga tgtgatggtc
    35761 aatcaataag atacaaattg tgtaaatcaa atattgcaac gctatggacc ataaaaacat
    35821 aaagtacagc tcccattgat ttttaccaaa ttatagcaca attggcgtat tattttttta
    35881 atttttcagg gattttgaat tcagaactca taccaaagca gcagccagat acaaaaaagt
    35941 cagataagtt agttaattgt ttaatgattg tttttttcat gaaagattcc tccaaaattc
    36001 atcaagtagt ttgtaatagg aagcaacgta gctatttcta aaacaatccc aagtagcaaa
    36061 taggtagaaa agtaagaagc tgagaaaaat aacgtaatga gagaaaaaat tagaaacatg
    36121 ctgatcgcct tctttaataa tgggggacgt ttctcgggtt caatgggatt gattgctgta
    36181 cctcttgatc catagcgaag cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt
    36241 gggaaggaga tagtcaattg cagatgtttc atcaccaagg gaacgcccca taaataaagt
    36301 agtgtccaca gcagacaaac gaattcagag tcggaatgca atccataagc atatctgcgg
    36361 atgcagatat aagaaagaaa ggtgattcct gtttccgccc atgagcctgt caccagagaa
    36421 aagactagaa cgaccgctag ttttccaaga ttattcaata aaacttctaa gacaaatttt
    36481 acttggacat acaccatatc ttcttccgat ttgtctccgc tgaataaggc ttctaataat
    36541 ttgttcacaa ctttatcttt catctcatac gtacctccac cacaaatttt atgctaaaaa
    36601 aaataaaaaa cgtcttttgt ttcataaaaa gtggttttga catcaaaacc gtcatgatct
    36661 cctaaaaaaa tcttcgtttt ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa
    36721 aagtactttt tatgacaagt ttgtgctttt tcgagtgatt tatgttaaaa aattactgac
    36781 cttataaaag gtgatggagg gattcttatg gaaaatgaac aattgtttga agtcggcgaa
    36841 acatatgcat ttaaatctga tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc
    36901 tatgaaaata gtgtgatgat acgtgtcatg aaatgcgcag tagaagacaa tgataccgct
    36961 cataatctgc actggcagtt agttgtacgc aaatcaagta caatagaaga ataaaaacag
    37021 ataaaatgtt gagagcataa agaggttgtg agtctcccgt ttagctctga gttacaagga
    37081 ataatgttaa aaaacagctt ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg
    37141 caggagaata ctgtttattt gaaaaaaggg ctgcgacaag acttttgtca cagcctttat
    37201 tttcatgaat aggagttact tttggctact tttagagaag tagcttttaa ggagcagata
    37261 aagtcaaggc ttttatttct ataaatgaag tagtaaaatg aagaacagat tttattacag
    37321 gaaggatgcc gataattgtg atcattgttg caggaacgat cggtgcaggg aaaagcacac
    37381 taactgaaat gcttgcccaa gatttagaaa caaaaccttt ttatgagaac gtggaagata
    37441 atgaagtatt gccgctattt tattctaatc cagaaaagta tactttttta ttacagattt
    37501 tctttttgaa caaacgcttt ttggcaatca aagatgcttt cagccatgat gacaacgtac
    37561 tagaccgctc catctacgaa gatagtatgc tgtttcattt gaatgcagat ttagggcgtg
    37621 tttctgaagt agaagtaaag cagtatgaag gtcttttaga aacgatgctg aaggagttag
    37681 aagaaatttc gccacaaaag aaaccagatc tactggttta tattcgtgta tcctttgaaa
    37741 ctatgttagc gagaatcaaa aaaagaggcc gggaatatga acagctagaa caagatcctg
    37801 aactctattc ctactataaa gaactcaatc gacgatacga agagtggtat gaacagtttg
    37861 atatatgtcc caaaatagtg atcgatgggg acaaatatga tttcgtagcc gatccggcat
    37921 gtggagaaca aatcgtacaa gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag
    37981 atgcccagtc gatccgctac actacaaata acccgaaagc aaagggaatc tttacaacta
    38041 aataatataa aaactaagca aattgctcac tcgcagatat ttctctgtga gcagcatctt
    38101 gcttagtttt tttgattcat tgagatcaaa tagtgaaccg attcgtatga taaaattgcc
    38161 ataaatgaaa tgtctgagtg agggccatga tcaaaaataa aatggaaacg atcaaaatcg
    38221 aatgggcgcc aaacagatga tgacagaaaa caaggacaat agctcctgcg ataaaggcaa
    38281 gaacgacact tagatagtgg aaaaatcgat caaaatctgc tttttcttta gaaatacaat
    38341 aatcatataa gttcgttgct acccctttta aatttccagt tgtaaataaa ttagtgtagg
    38401 cacgcccgtt gattttatca aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa
    38461 tcgttactaa tgcgggatgc tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg
    38521 cttcataata tagaacaaac aagcgccaga aatgtttttc tttttttcta aaataatcaa
    38581 ttagatactt tgttagaaga atccctaaga aaaagaaaag ggtagacaat acttttttac
    38641 caacacttag ccattctccg tcaaatgctt ggatgatcga tagaatgata ttgccagttt
    38701 gagcagaggc aaaagcacca tattggatat aagtataact gtccatggct cctccgataa
    38761 atgtcagcaa aagtcctatt gtacgcccct cgtgaaagga agtctgcatt tttttcatca
    38821 ccttttatga atatttttgt cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa
    38881 ttttcagaaa actaaaatca tatcgcttcc cattttcatt tttagaaagg tctgttagta
    38941 taaagaaaaa atagacaacg aaactttaaa taaccaactt gaaataaaca aattcaaaga
    39001 agaaagatgg gaaggaaatc aatcagttga atgaaaaaga aattctccga ttacaaaaag
    39061 aattgaaacg ctgggagaac aagtataaaa agcttcaaca agtctttcgg ctacaagaag
    39121 aaatcattgc tacctatgaa gcggcttatg gcgaagcacc cggacttatt gaggaagaag
    39181 tacaagtaaa agaaagtgaa ggaaacagct ctttctttaa aatgatggag aaacgttcct
    39241 ataatcgctt caatcaagaa caaaaagcag ctatcacata tgatatgaag aaacatcttc
    39301 gaatcatagc aggagctggg agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc
    39361 tgatgacgca aaaacaagta gacgaagaac gcatactaat gatcactttt acaaggaacg
    39421 ctgcaaatga actaaaaaaa cgagtagata atttcagcca gagaaaaacc gctgtccata
    39481 tcggaacatt ccatagtatc ttttttagga tatacaatga aatttgtcga aaatttcctg
    39541 aagtagcaat gcaaggaata caaggagact tttcaaagga tactgcacaa aaagtgaatg
    39601 ctgttttgca acagctgata cgtaaatata atctttatat atttgatcaa tatggagaaa
    39661 aaacgattgc ttctcggtta gactattggc agaatatgaa cctttcagta gaagaaatgg
    39721 ttcagttagt taaagaaaaa tatgattcca ttgataagaa tgcacgacag ccgatatcag
    39781 aacgactgta tggattgctc acggaactcc aagaacaaaa aagaagccag caattgctag
    39841 agttcaatga tgtactccaa aatctgaaaa gcgctttgga gaatgaggag atccagcatt
    39901 atatcggcca aaaatatgat tatctattca ttgatgaatt tcaagatact aatccacttc
    39961 aatgggaaat cgtgaaacgg atgacaaagg aaaacaggat caagttgatt gttgttggag
    40021 atgatgatca gagtatctat ggattcagag gatcagagcc agcatacatc aagaactttg
    40081 aaaaagaata tcctaccaaa actcttttct tattaactaa ttatcgttct cgggcagcta
    40141 ttgtccaagg agcaaataga ttgatcagct ataataaaaa tgatcggata ccaaaggcga
    40201 tgattccagc acaaaaagaa gcaggtgtta tggaagccta tttgttttct gatactaaag
    40261 cagaaagtca gtggctaatc aaacaaattc agacatttat tcaaaaaaat gggaaatata
    40321 aggaatcaat catattatac cgatcagctt cacaaacgca gcagcttgtc caatctctgc
    40381 tgaaaacgaa tattcctttt gtactggaag cagatagtcc atatgaaggt atctttggta
    40441 tcaagtcttt ccaacatttc tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc
    40501 aaaagaaaaa tcaggcctac cagcaattgc tccggcagct gatggcagac tgctacctga
    40561 aaaaaagtga atgcgatcaa tacttttctc ctaaatatca aaatatatcc gttgcagatt
    40621 atattttgtc gataagcccc aatttaaaat cgaaatctgc agaaatccga gagtttgagg
    40681 aagcgttgcg taaaacagac cagcatcaag aaatctattc gcttgttcaa agctatcttc
    40741 atctgccacg tatagctaag gaaatcgacc agagtgatca agaatggata cgagaagaag
    40801 taaagcagca tgctactttc agaagactcc aagccttatc gcaagaaacc caagcgacag
    40861 ctaaagaatt aaaaacccgc ttgattgctt acagacaagg aaaacttgat gcactatgta
    40921 tccagagcat tcacaaaagt aaaggtctta gttaccgaaa tgtctttttg attggttgta
    40981 acgaaggaag tttgccttat aacggagcga cagaaaagaa agtaatcgac tttaatgaaa
    41041 taaaagcaga accagtaacg acgatagaag aagaacgaag actattttat gttgcgatga
    41101 ctagagcaag aaatcgtctg tatctatgtg tacctcaaat gaaaaattca agaaaattga
    41161 aagtatcgag atttatcaaa gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga
    41221 gagggtgtga cagaagtggt cagtttcaag aaa
//
LOCUS       DACEKH010000014        41210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEKH010000014 DACEKH010000000
VERSION     DACEKH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.014"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..328)
                     /locus_tag="IW662_000664"
     CDS             complement(26..328)
                     /locus_tag="IW662_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0865511.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IW662_000665"
     CDS             complement(353..2446)
                     /locus_tag="IW662_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0865512.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IW662_000666"
     CDS             complement(2935..3699)
                     /locus_tag="IW662_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ0865513.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IW662_000667"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IW662_000667"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ0865514.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IW662_000668"
     CDS             complement(4704..5780)
                     /locus_tag="IW662_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ0865515.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IW662_000669"
     CDS             complement(5804..6397)
                     /locus_tag="IW662_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ0865516.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IW662_000670"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IW662_000670"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ0865517.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IW662_000671"
     CDS             complement(8067..8366)
                     /locus_tag="IW662_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ0865518.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IW662_000672"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IW662_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ0865519.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="IW662_000673"
     CDS             complement(10121..11032)
                     /locus_tag="IW662_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0865520.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="IW662_000674"
     CDS             11152..12009
                     /locus_tag="IW662_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0865521.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="IW662_000675"
     CDS             complement(12196..12558)
                     /locus_tag="IW662_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865522.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="IW662_000676"
     CDS             12904..14343
                     /locus_tag="IW662_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0865523.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="IW662_000677"
     CDS             complement(14350..16902)
                     /locus_tag="IW662_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ0865524.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="IW662_000678"
     CDS             complement(17016..18347)
                     /locus_tag="IW662_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0865525.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="IW662_000679"
     CDS             complement(18313..19791)
                     /locus_tag="IW662_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0865526.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IW662_000680"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IW662_000680"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0865527.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="IW662_000681"
     CDS             22401..23828
                     /locus_tag="IW662_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0865528.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="IW662_000682"
     CDS             complement(23920..24285)
                     /locus_tag="IW662_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0865529.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="IW662_000683"
     CDS             complement(24282..24647)
                     /locus_tag="IW662_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0865530.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="IW662_000684"
     CDS             complement(24839..25087)
                     /locus_tag="IW662_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865531.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="IW662_000685"
     CDS             25086..25214
                     /locus_tag="IW662_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ0865532.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="IW662_000686"
     CDS             25271..26965
                     /locus_tag="IW662_000686"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ0865533.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26969..27088
                     /locus_tag="IW662_000687"
                     /pseudo
     CDS             26969..27088
                     /locus_tag="IW662_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27170..27361
                     /locus_tag="IW662_000688"
     CDS             27170..27361
                     /locus_tag="IW662_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865534.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            27739..28872
                     /locus_tag="IW662_000689"
     CDS             27739..28872
                     /locus_tag="IW662_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ0865535.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28903..29223
                     /locus_tag="IW662_000690"
     CDS             28903..29223
                     /locus_tag="IW662_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865536.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29252..29716)
                     /locus_tag="IW662_000691"
     CDS             complement(29252..29716)
                     /locus_tag="IW662_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0865537.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29847..31104)
                     /locus_tag="IW662_000692"
                     /pseudo
     CDS             complement(29847..31104)
                     /locus_tag="IW662_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(31133..31450)
                     /locus_tag="IW662_000693"
     CDS             complement(31133..31450)
                     /locus_tag="IW662_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0865538.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31476..31796)
                     /locus_tag="IW662_000694"
     CDS             complement(31476..31796)
                     /locus_tag="IW662_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0865539.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32260..33675)
                     /locus_tag="IW662_000695"
     CDS             complement(32260..33675)
                     /locus_tag="IW662_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0865540.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33692..35008)
                     /locus_tag="IW662_000696"
     CDS             complement(33692..35008)
                     /locus_tag="IW662_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0865541.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35158..35856)
                     /locus_tag="IW662_000697"
     CDS             complement(35158..35856)
                     /locus_tag="IW662_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ0865542.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36145..37134)
                     /locus_tag="IW662_000698"
     CDS             complement(36145..37134)
                     /locus_tag="IW662_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ0865543.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37214..38047)
                     /gene="msrA"
                     /locus_tag="IW662_000699"
     CDS             complement(37214..38047)
                     /gene="msrA"
                     /locus_tag="IW662_000699"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0865544.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGAFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38222..39856)
                     /locus_tag="IW662_000700"
     CDS             complement(38222..39856)
                     /locus_tag="IW662_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865545.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39843..41177)
                     /locus_tag="IW662_000701"
     CDS             complement(39843..41177)
                     /locus_tag="IW662_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865546.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        12504 a         8476 c         6959 g        13271 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
    27121 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
    27181 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
    27241 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
    27301 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
    27361 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
    27421 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
    27481 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
    27541 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
    27601 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
    27661 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
    27721 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
    27781 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
    27841 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
    27901 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
    27961 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
    28021 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
    28081 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
    28141 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
    28201 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
    28261 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
    28321 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
    28381 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
    28441 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
    28501 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
    28561 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
    28621 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
    28681 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
    28741 atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca
    28801 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
    28861 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
    28921 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
    28981 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
    29041 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
    29101 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
    29161 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
    29221 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
    29281 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
    29341 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
    29401 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
    29461 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
    29521 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
    29581 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
    29641 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
    29701 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
    29761 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
    29821 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
    29881 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
    29941 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
    30001 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
    30061 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
    30121 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
    30181 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
    30241 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
    30301 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
    30361 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
    30421 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
    30481 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
    30541 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
    30601 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
    30661 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
    30721 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
    30781 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gcaagaaagc
    30841 aatcaagaca gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca
    30901 tgattcacca ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt
    30961 gatcattact gctaatgctc ctaagatcat taacggcata ctcaccataa atgagtcccg
    31021 aatcgcaact aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc
    31081 cataaatgca acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt
    31141 tcaatcgacg atacagatca atgatctctt ttgctaaatc tgtaaaagct atagatgtca
    31201 tcagatgatc ttgactgtgg accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt
    31261 cttgtgtcag catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt
    31321 tgattttttc atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg
    31381 catcgctttt tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt
    31441 gatcttccat ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct
    31501 tggtcgagta ctttttcacc attcatcatg ccatagtcag ccatattgat tacatctaaa
    31561 ggaatgcctt taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt
    31621 cctaaaagta aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat
    31681 acagcaaaga tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact
    31741 aggagacttg tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta
    31801 aacacgttcc tttctatact tacacttgta tataagcaag tagcgtgcca actttaaaag
    31861 cgctttaaaa agttgatatc atcacattta aggtctatac caagttgcat acacactaaa
    31921 aaagaaagcg cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc
    31981 acaaaaaaag ttgtgacaca aaacttgtca caactttttt caaataaacg gtattcaaaa
    32041 gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagatgttg tttttacttt
    32101 gctattctta ttacttgatg caggacagct ttttcacaac ctctcaataa acggtgttca
    32161 atcagctgac cctgggtatt agctcaaaac gctggattca caacctcttt tatttcaaat
    32221 aaccagtaaa tacaatgaac atctacaaaa aacttctatt tatttataga tttcgtcagg
    32281 caaatccaat tctctcttat caatcaaagt ctgataccac gaagcagatt ttttcaatgt
    32341 tctgcttcgt tcatcgttca attcgattcg aactaatcca tatcgattct taaatgcatt
    32401 cattggtgac acacagtcag tgaatgccca taacatatag ccttcgcaat ttgcgccttc
    32461 ttctgttgct ttgatcaagt aatgcaaatg ctgtccgata aattcgatac ggtaatcatc
    32521 ttgcacgatt cctttctcat ccatatactg ttcttcatat tctcttccca taccgttttc
    32581 tgttatcagc caagggatat tatgataatt ttcttttaga tacatggcta tatcatacat
    32641 gattttcgga tagatttccc atcccctgga attattcatt ttcttcccag gtaaatcaaa
    32701 gacttcataa tatcgttcag gatggaaagg tgtctcttca ttccattgat aacgaggggc
    32761 gcgaacgcgt ttaggataat attgattgat tccaaggaaa tctactgtat gatgctgaat
    32821 cgtttctaaa tcctctgttg caggatcgaa ataaatcgat tgtttttgac acaactccag
    32881 gaactcttct gtgtagtatc cttttaccat cggatcaaaa aatactcgat tataaaacaa
    32941 atcatacata taagcagctt tttggtcttc tggagaattg gatcgagcat agaccatttc
    33001 aggatttaaa atacagccaa tccttccacc ataatttccg ctgcggtaaa tctctactgc
    33061 tttagccgtt gctaaaactt tatgataatt ccacatcatc cacttttttg tgttttgttc
    33121 atgcggccag cgaacagcat ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa
    33181 tgtaaaccag ttttttaccc tatcaccgta acgatcaaag gcaatttgag catacttagc
    33241 ataaaaatca actacttttc ttgaagacca gccatcatat ttttccaaaa gaaaagctgg
    33301 gacttcataa tgttccaaac aaatcatcgg ttctacgtct gcttcgatca aagagtcgat
    33361 catttcactt atatgctgag catattcttc atctactata cagttctcat aatcagtaaa
    33421 aaaacgagcc caattgatag aagtccgata atgtgtcaga tggatggatt tcatcagatc
    33481 cacatcctgc tggtattgtt ccataaaatt agttgctacg gctgggccat acccattatg
    33541 ccaaacaaaa tgttcatttt tataccagct atctataaac gaatcctgtc cttctttttt
    33601 tcctttccaa ccttccgttt gccaagcaga agatgcagca ccaagaataa aattttttgg
    33661 aatagtaatt ttcatgattt cctcctgttt tttagatccc atcattttcc acacgatttg
    33721 ctacttttac aaatggcaga tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa
    33781 taactgcacc gatatttcct gctgttgcca gataagcatt gacgatcggc ggcattggcc
    33841 aagggaccat tacaactgct ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg
    33901 ttccagtgat aagaggcgtt aagataaacg gaatagctaa aattggattc agcatgatcg
    33961 gcataccaaa aataactggt tcattgatgt tgaagatccc aggagcaagg gataacttgg
    34021 tgatttgttt catatcttcc cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg
    34081 tgactccaga cccgcccata ctcatataca tatcccaaaa tggcatagta ataatgttcg
    34141 gtacttcttt tccagcttca aaagcttccg tatttacagc aattgccgct aaaagcaacg
    34201 gttcccgtat cggcttcacc atttgatttc catggatacc aatcacccaa aagacttgcg
    34261 caataaacat caatagcaaa atacccggta acccttgtac gacaccttct aatgggcgtt
    34321 gtacgacatt gtaaatgata tcataaaggt acattcctgt aagttctttg atgataaatc
    34381 ccgcagccgc gatacccgat acagtcagaa tcgtcgggaa taaagcagaa aaactagcag
    34441 aaacattcgt tggcacggta tcaggcattc tcagctgtag tttcttctgt ttcctcaacc
    34501 agcaataaag ttcaatagaa aggatggaga ccaacattcc caaaaataac cctttcgtat
    34561 ctgtatattg tttagccaat acgttttcta ctataacagt ctgattttca ttcactaatt
    34621 cataaacagt tggattaaca gataaataag acatcactgc gatgatacca gggaatacac
    34681 catccacttt attcagcttg gctaattcga tccctatcag aaaaacagca tagatggtca
    34741 agaaacttag tgtgacatag ctgatggcag atgcgatagg ctttaattct tctaaaaaac
    34801 gcaagaaact tatctgtgct aagccatttt ccgagtcaaa gaccattgct gaaaacaatg
    34861 tgccaaaagc acccgtaata attaccggaa tcaatgcagc aaatgcgttt tttatcacca
    34921 tgatatacct taaattattg attctcgttg ccacgttccc taaaacattg gctaatttat
    34981 ccataaaact cattccagat cccctcattc ctgcttcttc tccttgacgt catctctttt
    35041 tgtctattag tcagcgctta catttcgatg aacagaaact atttactcct cttttttacc
    35101 aaatataaaa caaaaattca tctgatgtat ttatcataaa atacatcaga tgaactgtca
    35161 attagtttct ttttctccta ttattttctg taattctctc cgatttattg ccatatggac
    35221 agtcattgca tcaaacgcag caagtgcatt ccgcctttcg atggcctgaa cgatctctgt
    35281 atgaatatcg atcgtctcga ttttcagatg ctggatatca taatttttat taaacaaagc
    35341 aattgattca ttaatgattg ggataatatg cagcaaggct acgtttccgc tagcttctgc
    35401 aatcatcgta tgaaaatcga tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga
    35461 agtttcaatc ttttgcgtta attttttcat ttggacgatt tgattcttcg ttgcatattc
    35521 cgctgccaaa cctgccatct taggctccaa taaatatctc agttcaaata agtctcgtat
    35581 catccgatcc tgatcttcaa tcaaagaaaa accgagtggg tcattggcaa tccctcgctt
    35641 atcactgaca aatgtccccg ctccttgccg aacttcaagg atattcctgg aagctagagc
    35701 ccgcacagct tctcgcaccg tgcttcgccc cacctctaaa taatcggaca attcatattc
    35761 attcggcagt ttatctccta cttgcaactg catttccttt aaatatctaa ggatcttgtc
    35821 agtagttact tctactaggg ttttattttc ttccaaacca catacctcct tttcatctga
    35881 tgtatttttt cgtaattatt ataacacgtt tgaatcatct gatgattttt tgtatacaaa
    35941 attgattaat ccgttcatag acatacaaac gaagaagata tttaccataa aaaagactag
    36001 gataaaattc actcctagcc ttcgatcatt tttattttga acgacaaaaa atatgatgtt
    36061 caataacaga cgatcttcat tcgattcttg atgtcatctt ttgttttttt taatatatgg
    36121 ataaaaagtt tgattacatc gtttctattc tagaatttgg tcgattctca attcttcttc
    36181 tggtgagaaa tctaaattat ccatcattcg aacactttct tgtaactgac ttaaccgact
    36241 tgctccgacc agtacacttg taacagattt ttgtcgcaaa ttccatgcaa gggccatttg
    36301 cgctaatgat tgtcctcgtg atacggcgat tgtttgcagt gctttgattt tttccagagt
    36361 tgatccaact tgttcttctg aaaggaatgg tatttcctta cgatgtgctc tagaatcttc
    36421 aggaatacca tgtagataac gatttgtcag caacccttga gcaagcggac tgaacgtgat
    36481 tgcccctagc ccttcttctt ctaatactgg agatagatcg tcttctagta gtgcacgaga
    36541 aaacatattg tagcgcatct gatgaataat gaacggtgct tcctttcttt ttaaaatttc
    36601 agtcattttt tttgtatctt ctccattata gttggatata ccaatatata atgcttttcc
    36661 ttgtcttaca agttgcatca aagcttctgc tgtttcttcc aaaggtgtat cagggtctgg
    36721 gcgatgatga tagaagatgt caacataatc tagccccatt ctttggagac tttggtcaca
    36781 acttgaaatg atggatttac gagagcccca ttccccatat ggcccttccc acatatgata
    36841 acctgctttg ctagaaataa tcagttcatc acggtaggct tgaaaatctt ctctaaaaat
    36901 acgcccaaag tttttctctg cagctccagc agggcctcca taattattcg caaggtcgaa
    36961 atgggtaatt cccatgtcga atgcgcctct taaaatctct ctttgattat ggataggatc
    37021 ataatcacca aaattttgcc ataatccgag agataaaatc ggtaatttca aacctgaatg
    37081 accggattta cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc
    37141 tcctcctcat caaatctttt taaaacgctt tcttttttct atttttagtt tactacattt
    37201 taaagaaaag gagttaatca aaatgatttt ttcgtatatt tttttgccat ttgtttttta
    37261 ctcgttgata gatcagaaat tgattacgtg cttgctggat ttttttataa cgtttcggtt
    37321 gttttttgta aaattgctga tgatagtttt ctgctggcca aaacgtaccg gctggttcga
    37381 tttttgtgac gattggttgg tggaaagctc ctgaactgtc tagtttctgt tttgattgtt
    37441 ccgctaattc tttttgtcgt tcgccttgat aaaaaatcac tggacgatat tgcttccctc
    37501 tatcttgaaa ttgtccaaat gtatctgtgg gatcagtaat ttgccaatag attgtcagca
    37561 actcgctata acttatttct cttgtgtcat aaatgatctc cacagcttcc acatgacctg
    37621 tgtaaccacc acttacttga tcataatttg gtttttcaac atgtccgccc gtgtatcctg
    37681 acaaaacaga taggatccct tttttcgatt caaacggctc gaccatacac cagaaacatc
    37741 cccctgcaaa tactgcacgt tcttgatagt cagcatcttg catggcatgc ttggaaaagt
    37801 catatttttg ctccttatca aatccttccg taatcaataa ataaaagtcc attacatcag
    37861 gcgtcagatt cattcggata gctaatggac gtagtgttgc ttctagtttt gataactgtt
    37921 ctttaacgga tatattttct cttatgtcat tctttgtctg aatcaatact tttcgttccc
    37981 aatctcgtgt attcggattc aaaatcagat tataaagtgt ttgaatgaat atttgttcct
    38041 catccataga ttctcctcct ttccgaagaa gacaagctag tgactttagc tgtaatttat
    38101 cataaagtga ggaaataagc gattacttca tccccctaca gatttttcgt ataaaaaaga
    38161 gagaatcctt atcgacagat tctccttttt aatcaattta ttttataaat gaaaagttaa
    38221 tttacctttt atggaaaact attttaaaaa ataaacataa atatgcagtt aatgtaacaa
    38281 gataagaaat aatagtcgtt actgtataac ttgtgcttac tttaatatca gcaactcctt
    38341 tgactccgct tatttgtacc aatccattag cattcacctg ctcctttaca ggttgatgat
    38401 ttatttctac attcgttcct aaataagcat atacaggaag tgtgattact tggctcttct
    38461 cattagttac tctatatatg atagaactac cagttgtttt ataaggaagg tttacccatt
    38521 gactgccaat ctttgcttcg tgttttatta tatgctcctt attctccaat gaagcgatag
    38581 gagaataatc atataaaaaa taatccattc ctttagtggt atacgcctct actgtttctt
    38641 tgtttacaac aatatgtccc ggctgagaaa ttattttttg attgaaattt tttttactag
    38701 aaacagttaa agtgattaat aacaaaacag tcataaataa ataaaatagt tctcttgtct
    38761 tattgtatct tctatttttg aaaaatatca tcgttaatat cagagaagag aatattattt
    38821 gcaagcttaa gatccgccaa ggaaattgta acaatttggc tggcgtattt tgcagaagat
    38881 accatggcac caaactagtt gtagctagcc aagtcaaaca cgtaccataa taaatctttt
    38941 tttctatgtt tgttaatttt ttaaataaaa tcaggggaat acataaagat aacagaccaa
    39001 ctagtcccat tgagtaagta gttaagtcgt tgttcatagc ttcagtcaga ctatcgaaca
    39061 cattagatgc atgagcatat aaatttgtca cgctaggttt gttgattttt tggtagagca
    39121 tttgttcaaa catcggatac caaaaagcac tcgtcagaac caatgtcatt cctgccgcac
    39181 taaataattt tttaaatata gcaataaaat ctgcattctt gaaaccttta gaaaccaaac
    39241 gaataaaaat aaaaatgata attaatgctg tactcattaa tgttgatagt acatggctgt
    39301 acactagcaa actcatactc aatgctagct ttacagacgg atacttttct tcttttccag
    39361 agatgatttg ataagcataa aataatacag ggataacaag tgttattgcg atagcttcgg
    39421 caattgcgga acgatagtag atgtctactg tccgataagc agagaaaata tagagacaac
    39481 taaataaaaa tgccgctttt ttttgtttta aaaaccgctc gccataatga taagctacga
    39541 gtgttgtaca caagttcaat aaccaaacgt aaataatata gcttacaatc aaattttttg
    39601 tcatccaata taacattaca gctggaaaaa aagtcagaaa gggataaaaa taatttacac
    39661 catatccagt ataattgaat gtctcataat taatcggact ggtaaaaata gtcgataacc
    39721 ccttgattcg agataagtga aaagaaagat ctccagtggt ataaatactc tgtgtatttt
    39781 tgaaaaagat gaaagaatat atactactaa ctataattaa tccactcaat attactactt
    39841 ttttatttat tttcattctt cttgtttatc tcctttttag tcacatatcg agaatctttc
    39901 aacttattag gtgcaaatcg tttaaaactt gaatacacca ctaaacaata agtcagaata
    39961 ctgatccata aagccatgag agcagttgga gtatattttg ttgtaatttc tatcttattt
    40021 tctccagcat ttacatccac attcactaag ccatgttttt tataagagtc atttacgact
    40081 ttaccgttta cttttacctg tgtacctaaa taagcatata ctggtactgt cactttttgc
    40141 tttttgttgc ttgccactgt gtaagtaatg ttattatctg cagctttata cttccctttt
    40201 tcccacgatt ctcctacttt tacttcatgt ttttctaagt gaggcctttc ttttatcgct
    40261 tctgttgggg cgtaatcgta taatcctccc atatctgatg ttgtataacg tgggagtgtt
    40321 tcttcattga taacaatatg accgtgtttt gattggataa cttttgcgta attttctttt
    40381 gctgctatag acgtgacgag tagaaacacc acagtagcgc caatgattaa ccatgaatat
    40441 tttttattca tagtcttttt cataaaaaca agcgttatga tcattgaaga aaaaaagatt
    40501 tggataccta agatacgcca aggaaactgg agcaagctta tcggtgtttt ttgaaacaaa
    40561 aaccatggaa ataaattagt tacgcatacc catgtagaaa ctacgccaac atagatgatt
    40621 ttttcttttt tctccatttc tttcagtatg aataaaggaa aaatcaacgc aaccaatcct
    40681 aataacccca tagtgtatgt tgtaatatcg ttatttaacg cttgaatgat actatctgaa
    40741 atagaaagtg cttctagctg caaatctgtt ttgaagggac tgtgtatttc ttgataattc
    40801 atctgctgca acataggata ccaaaaatag ctagttaatg cacaagtcaa aaaaacagcc
    40861 tttactaaat tcacgaccac aaattttata tcgtttttta tttgcttgct taaaaacaaa
    40921 cgaactataa tcatacaagc aatcaatgca ctacacaata gtgtagttaa gacatgagta
    40981 tacacaagta gacttatact gacagataaa gcgatgactt ttttttctct attgaatatc
    41041 aatccataag cataatataa aacagctggg ataatcgtaa gagcgattgc ttctgcaatt
    41101 gcggatcgat aatagatatc aactgttcga taagttgcaa aagtatataa acaactaaat
    41161 aaaaaagcag catctatctt tttaaaaaac ttttctccat aataaaaact
//
LOCUS       DACEKH010000015        39696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEKH010000015 DACEKH010000000
VERSION     DACEKH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.015"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            99..746
                     /locus_tag="IW662_000702"
     CDS             99..746
                     /locus_tag="IW662_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0865547.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IW662_000703"
     CDS             1139..3784
                     /locus_tag="IW662_000703"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0865548.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IW662_000704"
     CDS             4094..5407
                     /locus_tag="IW662_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0865549.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IW662_000705"
     CDS             5397..6050
                     /locus_tag="IW662_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0865550.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6776
                     /gene="radC"
                     /locus_tag="IW662_000706"
     CDS             6105..6776
                     /gene="radC"
                     /locus_tag="IW662_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ0865551.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6671..8023)
                     /locus_tag="IW662_000707"
     CDS             complement(6671..8023)
                     /locus_tag="IW662_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0865552.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(8089..9378)
                     /locus_tag="IW662_000708"
     CDS             complement(8089..9378)
                     /locus_tag="IW662_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0865553.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(9465..10169)
                     /locus_tag="IW662_000709"
     CDS             complement(9465..10169)
                     /locus_tag="IW662_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ0865554.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            10369..10635
                     /locus_tag="IW662_000710"
     CDS             10369..10635
                     /locus_tag="IW662_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0865555.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(10803..11033)
                     /locus_tag="IW662_000711"
     CDS             complement(10803..11033)
                     /locus_tag="IW662_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0865556.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            11105..11260
                     /locus_tag="IW662_000712"
     CDS             11105..11260
                     /locus_tag="IW662_000712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865557.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(11569..11793)
                     /locus_tag="IW662_000713"
     CDS             complement(11569..11793)
                     /locus_tag="IW662_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0865558.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSPGNDPHPPKG"
     gene            12060..12395
                     /locus_tag="IW662_000714"
     CDS             12060..12395
                     /locus_tag="IW662_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865559.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            12628..13569
                     /locus_tag="IW662_000715"
     CDS             12628..13569
                     /locus_tag="IW662_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ0865560.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEKGIK"
     gene            13571..14461
                     /locus_tag="IW662_000716"
     CDS             13571..14461
                     /locus_tag="IW662_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ0865561.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            14504..15304
                     /locus_tag="IW662_000717"
     CDS             14504..15304
                     /locus_tag="IW662_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0865562.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            15319..16170
                     /locus_tag="IW662_000718"
     CDS             15319..16170
                     /locus_tag="IW662_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0865563.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            16167..16526
                     /locus_tag="IW662_000719"
     CDS             16167..16526
                     /locus_tag="IW662_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ0865564.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            16541..16702
                     /locus_tag="IW662_000720"
     CDS             16541..16702
                     /locus_tag="IW662_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0865565.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            16699..17025
                     /locus_tag="IW662_000721"
     CDS             16699..17025
                     /locus_tag="IW662_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865566.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            17022..17837
                     /locus_tag="IW662_000722"
     CDS             17022..17837
                     /locus_tag="IW662_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ0865567.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            17976..18287
                     /locus_tag="IW662_000723"
     CDS             17976..18287
                     /locus_tag="IW662_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865568.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            18284..18514
                     /locus_tag="IW662_000724"
     CDS             18284..18514
                     /locus_tag="IW662_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865569.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            18521..18724
                     /locus_tag="IW662_000725"
     CDS             18521..18724
                     /locus_tag="IW662_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ0865570.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            18721..19020
                     /locus_tag="IW662_000726"
     CDS             18721..19020
                     /locus_tag="IW662_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865571.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
     gene            19263..19676
                     /locus_tag="IW662_000727"
     CDS             19263..19676
                     /locus_tag="IW662_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0865572.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILVALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            19689..20075
                     /locus_tag="IW662_000728"
     CDS             19689..20075
                     /locus_tag="IW662_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865573.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            20626..20850
                     /locus_tag="IW662_000729"
     CDS             20626..20850
                     /locus_tag="IW662_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865574.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            20868..21296
                     /locus_tag="IW662_000730"
     CDS             20868..21296
                     /locus_tag="IW662_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865575.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            21274..22692
                     /gene="terL"
                     /locus_tag="IW662_000731"
     CDS             21274..22692
                     /gene="terL"
                     /locus_tag="IW662_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAQ0865576.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            22704..24239
                     /locus_tag="IW662_000732"
     CDS             22704..24239
                     /locus_tag="IW662_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0865577.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            24319..25197
                     /locus_tag="IW662_000733"
     CDS             24319..25197
                     /locus_tag="IW662_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ0865578.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            25199..25516
                     /locus_tag="IW662_000734"
     CDS             25199..25516
                     /locus_tag="IW662_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865579.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            25565..26266
                     /locus_tag="IW662_000735"
     CDS             25565..26266
                     /locus_tag="IW662_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ0865580.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            26281..27171
                     /locus_tag="IW662_000736"
     CDS             26281..27171
                     /locus_tag="IW662_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ0865581.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            27194..27409
                     /locus_tag="IW662_000737"
     CDS             27194..27409
                     /locus_tag="IW662_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865582.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            27421..27762
                     /locus_tag="IW662_000738"
     CDS             27421..27762
                     /locus_tag="IW662_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865583.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            27762..28133
                     /locus_tag="IW662_000739"
     CDS             27762..28133
                     /locus_tag="IW662_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865584.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            28126..28521
                     /locus_tag="IW662_000740"
     CDS             28126..28521
                     /locus_tag="IW662_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ0865585.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            28523..28900
                     /locus_tag="IW662_000741"
     CDS             28523..28900
                     /locus_tag="IW662_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865586.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            28901..29509
                     /locus_tag="IW662_000742"
     CDS             28901..29509
                     /locus_tag="IW662_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0865587.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            29509..29850
                     /locus_tag="IW662_000743"
     CDS             29509..29850
                     /locus_tag="IW662_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865588.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            30092..32881
                     /locus_tag="IW662_000744"
     CDS             30092..32881
                     /locus_tag="IW662_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ0865589.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVESVK
                     DAWSGIKEWFSNTWQGIKNGATGLFDKTVETSMNAVDSVKNAWSNTKQWFSDIWQSIK
                     DSATEKWNEIKGSIMEVAGPLITGLKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LTPVLFITSMISGGWEETKNNMIGVWNNIKESALNIWESIKNIFVSYITNIYFAALNI
                     WTGFKLTLINIWNEVVSQAKSIWINVKYFFINLWIDIKYFAIQKWIELKFGIIQTWID
                     LKYNAITTWNNIKQFFKDTWQNIKDIAYNTWISIKNSMINTWNNIKESFWNIVTGIVN
                     SAENAWTNLKNGVSKAINRVKEIFDSLREINLFEIGKNIIDGLINGVVEKWNALKKTI
                     KGIAGSIKDSIKGALGIHSPSRWMRDMVGKNIVQGIIVGIDKEKSKLDQTMTDLVKTP
                     SVQPVITGSNSQPVVQAKQNTSSNAVNEIHLHLNVYGDLPDSMIKQIAKKMKTELTRQ
                     MKRDADAVGGTLYAT"
     gene            32871..33611
                     /locus_tag="IW662_000745"
     CDS             32871..33611
                     /locus_tag="IW662_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0865590.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKAYYKNVTRKIECYYKAPSIDLVQEWEDRITEWLDMNSYSDFILYYDQQYIYQ
                     AIVTEAPEFKGTRKTGNIVPFEFTVSLRPFKENYSGRFAIQQIKAFELFNPEKYASKP
                     LIKLSGSGDASFYINNDKYDLKLLDRELYIDSKLEESYRKLDDNLEHQDHVTLFLDFP
                     FLYPGKNEIKWTNNIHSFEIIPRWWRKV"
     gene            33608..36319
                     /locus_tag="IW662_000746"
     CDS             33608..36319
                     /locus_tag="IW662_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0865591.1"
                     /translation="MIPRIYSPTETDFSTNGLGILKDTTKCEIYEVANGKYELELEYP
                     LGTRFDEYFENDYQIKAKSNDQEEYHIFFIDDKDIDTFLDTVTIYAQSRTNRLGRRAV
                     TFAGVDSKTGREAMAIIENNMDKKSDIRLYSDITTVSSTTFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRDNIGTIRYGKDLSGLKVKLDWTGVKTRIIPYADPQS
                     DAGTTSRIYGSPVDSEYINNYPDVYTEHVQFTEEQGVKDVNSLNKIAKNYFKTINPGC
                     DKPKISITVEFDKLTDTEEGKEFAKIRNYGLFDTFKIYHRKYKLYFESKVSGVQYDSL
                     SEKVLKLEAGDAQVAFYQQQAVTIQDKLKDYATNNYMSSFNDYVSSMIAGQGNAGGYV
                     VLWPKEKPSNIFIMDSPDLNKAKEVLRMNKNGIAFSKNGWNGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSNSSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEKRAWKIWTMLKARGYSEYAAAG
                     ILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPLVGSPTWNGREYVQRLMNTAGI
                     QEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKSINDAKSAAYAFEMNFERPASA
                     HPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTVTSECGWRTSPINGGQEFHNGI
                     DLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIKHNNGKWTGYAHLSRIDVSVGQ
                     KVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVDFENPRNYIQF"
     gene            36332..37240
                     /locus_tag="IW662_000747"
     CDS             36332..37240
                     /locus_tag="IW662_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ0865592.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            37240..37857
                     /locus_tag="IW662_000748"
     CDS             37240..37857
                     /locus_tag="IW662_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865593.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            37861..38310
                     /locus_tag="IW662_000749"
     CDS             37861..38310
                     /locus_tag="IW662_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865594.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            38310..38804
                     /locus_tag="IW662_000750"
     CDS             38310..38804
                     /locus_tag="IW662_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865595.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            38818..39249
                     /locus_tag="IW662_000751"
     CDS             38818..39249
                     /locus_tag="IW662_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865596.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            39251..39388
                     /locus_tag="IW662_000752"
     CDS             39251..39388
                     /locus_tag="IW662_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0865597.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            39425..>39696
                     /locus_tag="IW662_000753"
     CDS             39425..>39696
                     /locus_tag="IW662_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865598.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT        13801 a         6100 c         8243 g        11552 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttccggaata tataagttag
     6661 tacacataaa ttatataagg aaatttactg ttatattgcc atctggaaat actattatgt
     6721 tatcgataat tgaacgccat aatgcatttt tttcttcttc cgaagattct ttataaattt
     6781 catcgaattc ttttgataga ataaattcga cattttttat atctatttta gctggctcta
     6841 cttcaatttc atttaattct ttcattaaag cttctcgatc ttttttgtat tcctctaagt
     6901 ctataacttc attcaagtag gctttcttca atctatctaa tttattctca atatttttct
     6961 tactattttt ctttttaata atctttggtt gtttttttga atagtttatt tgtatttgtt
     7021 caaatttttg tttagtagct tcaaaaatat aacgttctaa atttttttcg taaaaagcgc
     7081 gcttatttgt gcaacgtaat ttattatttc ttcgttttac acatctataa caagtccttt
     7141 cgtatataga ttctgtaccg tcttttttct tatatcttgc tttaatagtt tgaccagcta
     7201 ctgatccctg acagtcagaa cattttaata aacctttaaa aatgtaatca cgttttgcat
     7261 ttgtttttaa atttttaggt aaggcttttt gaacagcata aaaaagtttt ttatcaataa
     7321 taggaggaca gtaattttta ttatttctta attcaccaat gtattttttg ttcgtcaaca
     7381 tatttttaac actttgataa tctctaacga ttccatactg agagcctaaa taataaacag
     7441 tttttcttaa actaccacta gaaagaaagt agttgaatat atctatcacg ataggagcat
     7501 catcatttat tactaatttc ttattttcta tagagtagcc taacggagtt ttcccgctta
     7561 ctacttcacc ttgtgcaatt ttatttgcca taacagcttt gatgcgttct gaatcaattt
     7621 gggcttccaa ttcagcaaaa ctcattactt gtgcaatgaa agttctgcca tacgctgtag
     7681 ttgtatcata atattgttga gaaacagcat tccaagaaac attgtgtttt tctaaaattt
     7741 cttgagtgtt taaataatgt cgtagatttc taaaccatct atctaatttt gtaaataaaa
     7801 ttagatctat tttattttct tttacatctt gtaatagttt ttgaaattca tcacgattta
     7861 ttttttgacc agaaatacca ccgtctacat attcattaac tactttcaaa tccttgtgtt
     7921 gttcgatata ttcatagagg gtttcttttt gttctctaag actatctcca tgtttggctt
     7981 gttgttcagt tgatactctc atatataatg ctactcgttt catgttatct cctttcgaat
     8041 gtatgttctt atcgttgtaa aagaaaagcc cgaaggctaa tctttttact acacatctgt
     8101 taattctaat gatcttctgt attgttcagc ttgagctagt ttagtaaaag atacagttcc
     8161 gttataattt tctaaaacaa ctttttcaat ttcatcaaga gatactttaa agaattcttt
     8221 tcgttgatta actttattga cttcgttttt tctaaaatgc gaatgtagtt gattttctaa
     8281 tgttggtgca tcttctgaaa atatcattgc atgaacatca aattcaaaag gtacagaagc
     8341 gctactaagt tccttaattc tatccatagg ttctaaccgt ctagtcattc ctattttata
     8401 gacatcttca ccgaaagatc cgatattact gatgacataa acatagccag ctctagtatt
     8461 aagttctctt tgcaatacat cttctttgac agtttctagt tctcttaatt tctcttccag
     8521 ttctttaatt ttatcagcat agagttcttt ttcaacatca gcactagctt tttgcatgta
     8581 tgccattaac ttagttatct ctgagttaaa ctgtcgttct tctttatcaa tgcgtttctt
     8641 ttcacgttct atttcacgtc ttaccttttc ttcttcaatc atttgttctt ttattgcttt
     8701 ttgctgttct ttttcatcgt taactttaac tatgtatgaa tagttgagtt ctaattcttg
     8761 aagttttaat tcaagtaatt cttttttgaa ttctgcttca tcgtttttaa aaagcttgtt
     8821 tattttatca aaagttctta taattttttc tctagttgaa tctacgttag aaatagtaag
     8881 tttattgaga agtccagtta tttctgattc taatggtata agcattttct ttttaatatt
     8941 attagcttct ctattattca aataagaagg aatattaatt gcaccgtcta aaataaaatc
     9001 cttttcttta atctttaata aagaaagttt gttttttatt tcggatgaag aagtttcatc
     9061 tatattcgga ataattactg atttatagat agtgaccaga gatgtgtcat atttttgatt
     9121 aatttcatcg tctaaccgct ggctttcagc attaagaata tcaattttgt tttctagcgt
     9181 tgacaagttt ttattcaatt tacttatgtt tttctctttt tcagatataa acgtttccct
     9241 agcatttaat tcttgagaat attcctttac cttactctct aaggcagaag ttttatcttt
     9301 taatttttta gcttcagata atttgctact acccataatt attaaaacta ttccaactaa
     9361 aaacaatagt actcccattc aaaacgcctc gcttctttta taaaataatt gtcaaaatca
     9421 tataaaccct taccgaagtt gacggctccg gcatgggaat ttttttaaaa tacaactttt
     9481 ccgataattc taatctgttc tggcgataca attctatctt cgtataagtc gtttatagaa
     9541 cgtaaaatga ttgtttgatt ttcgtaatct ttatatatct ctttacatgt aactccgtca
     9601 ccacctaatt ccacaatagc aatttcccca tcttctacgt ccggttgttc tttaacgaat
     9661 acgaattgtc cattcctgat aacaggttcc attgaatcgc ctataatagg tactgcgaag
     9721 tcagcagcac ttggaactct ctcaaattct tcctcttcga caaccgtatc accgtaagta
     9781 agttctgttg gattggcagc cgaagccgaa ttgtgaacag ttgggatttt ttctttttta
     9841 ggaaaatcta ggacattttc ttgtttttgt tcttctaatc gattacttgc aaattcatag
     9901 gtagcaactt tattttcgtc gttaagctga ttatatatag gaaggatatt atatatctga
     9961 ttagatatag ataaatcaat attcatcaaa tcatctatat caacattgaa tatcttagct
    10021 atctcattta aggtttttaa ttttggtgta tattttcctt tttcccattc gcttattgag
    10081 gatgaacttt ttcttcctag tttttcggct agttcttgtt gatcaatatt atacttttct
    10141 ctaagaaact ttagattttc agcgaacata gtaacacctc cttgagttaa ttgtaacacc
    10201 gtttcggaaa aaatgtaaca ggtttcttag aaaatatttt agtgttttta aagaagtatt
    10261 tacgcaaaat agaaatcgat ttcaggaaaa ccgaaatatg cacttgactt cggaaaaacc
    10321 gaagtatata atttagtcag aaagttaaag agaggaggcg aataaaatat ggataccgcg
    10381 aagaaagaaa aatatacatt gagacaatta agagctttaa agggttattc aaaagaagaa
    10441 ctgtcaagga aaagcaaagt gacatcaaga acaatattta tttacgaaaa cgatgtagat
    10501 aaaatgagga acggaaagta cgccactcta gaaaaaattg ctaaggcatt gggtgttaga
    10561 gtttccgata tttttttaga ccctgattcg gaaaaaccga aatcaataac aggtgaaaca
    10621 aaacaagtca gctaggaggt gtgaatagtg gaagagaaga atataaaagt accgactctt
    10681 gaagaatata caaaaattgt tgtcgaaaca gacgagcaga acccgaaaac aatcgcagtt
    10741 attacggcga atgatattga atcatgcgaa ggttttcgag ttcgaatgac ccctagatat
    10801 gattaatgtt ctttatcacg tggaggtata ggatcgtttc caaaactatc ttttgattgt
    10861 atttgtccat tctttttttg gacaataaac tctgattgct gttttttaga aatcttttta
    10921 ccatactcaa cagcatctga tttattatca aatagttttg tatcttcgga attaccttca
    10981 ccacgtacag cccatttgcc atttctagga gatacccatt gatttttacc cataacaatt
    11041 cacctcgctt tcaagttaat tctattaaga ggtgaaaaga aaacaaactg atggaggtga
    11101 gattgtgagt aacaagccaa aaatcacttt gaatggcaca cctaatcttc aaagattagc
    11161 agatgaaatg gctagaatac gttctagaca ttggggagtt actgtggtgg caaccataat
    11221 tgaacaaaaa gaaaagccca cacagccggc aagcaagtag tgggcaccat agaacaataa
    11281 tattatatca cacactcgcc acttccgaaa agaggagttg ctgctctcct ttcaagtttg
    11341 ccaaagtcca cgtttcttca ttttggcaac tcctcttctt ggaggtggtg acggcgagag
    11401 acgaaggagg gatgacatga aggaagaatc aaaaataggg attttgaatg agaacaatta
    11461 cacaagatta attattgaaa cagaagaagg ggaaaaagta gcagaagtta cattgaccga
    11521 tgcaactcct gctgacggtt atcgtattcg attaactcct aaatatgatt aaccttttgg
    11581 tgggtgtgga tcgtttccgg gactatcacg gctttgaatt tttccatctc tgccatgaat
    11641 aaatagctca gatccttgat ttttagaaat ctctctagcg atttttactg cttctgattt
    11701 tgtattagta tgtgcagtcg ctttagaatt tccagcacct ttgacattcc aaccaccatt
    11761 ggaattaggt acaacatgtt gattttttcc catagttttc acctccattt ctatagaaat
    11821 attttatcaa atagaaatgg agaaattcaa taaacgaaag gagggatcaa atgacattac
    11881 aagagcatca acacttgatg cgaaaactaa atcaggaata ccacgaggaa atgaaaccta
    11941 gattgctagg ggaacaatgg cgtgaacgcc agaagaagtg gttagaaata aaaaagtgac
    12001 tcagccgtcc aaagcaatga gtcacaaacc aaaaattata cctaaggaga ttttatcaca
    12061 tgaataagaa aattgaaaat cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg
    12121 tttctattat ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt tatggtgaga
    12181 caacagaaat tttactttgt cttgctatgc aagaagaaca tttagatgaa aatctcccat
    12241 tgtcagcaca tattatgcgt agaattgctg tggatgctta cgaacaagcg aaaaatgaag
    12301 aagaaaatca accttcaaat catacttttg ttattaataa caaggaagat ttagctgatg
    12361 tgatgactcg tattctcaag ggggaatttc aatgatgaat gaaaaaacaa ttgatctact
    12421 cattatttta tttttcacgt tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt
    12481 attcgcaatt accatgcttg tgctttgcgt gttagcaagc agaaaagagg ttaaacatga
    12541 acgagttagc aaatcttgac aactatttaa ctgatcctga ttatgctaaa ccaccatatg
    12601 aagcgccaat tgatgaggag gatgaagatg agtaaatcta ccttagaaat gagccatcaa
    12661 gaatggctcg aagaccgcaa aagaggtatt ggtggctcag atgttgcaac cgtacttgga
    12721 ttaaacaaat acaaatctcc ttatcaattg tggcttgaga aaacgggtca aattgaattg
    12781 aaagatttag aaagcgaacc agcttattgg ggtaatgttt tagaagaagt ggttgctaaa
    12841 gagtttcagg aacgtacagg caaaaaagta cgcagaagaa accaagtatt tgaacatcca
    12901 ttacatccat ttttaagagc aaatattgat cgggaagtag tgggagaaaa tgccattctg
    12961 gaatgtaaaa cagccaatca atttctcgga aaagagtggg aaggtgaaga ggtaccgctt
    13021 agctatctct gtcaagttca gcattacatg aacgttttaa acaaagacta ttgttatatc
    13081 gctgttttga tcggtggaca aaaattcatt tggaagcgga ttgaacgaga tcaagagctg
    13141 atcgatacaa tcactgaaca attagtagag ttttgggaaa cgaacgttct tggaggtatc
    13201 gagcctgtaa ttgacggaag tggagcgact gctgacttct taaaagaaaa atatgcagat
    13261 gtagaagaaa atcaaacagc tctaccatca cgttttgatg aacttatcga gcaaaaaaga
    13321 gaactcaaac ggacgaaaaa agaaattgaa tcagctatcc gtcaagtaga caacgagatt
    13381 atcagcgagc taggtaaacg tgaagccagt atcggtatca ctcaaaaaaa catcatcagt
    13441 tggaaatttg tccgtacgag acgtatgaac tcgaagaaac tagcagagaa atatccagat
    13501 gtcgcaaatg atgaagagat ttataacgtt actgaatcaa gaaggctaac cgaaaagggg
    13561 atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa ttggcagaaa agccacagaa
    13621 acaagttgca ccaggacagt tagggcttaa agctctaatg aatacaccaa caatgagaaa
    13681 gaaatttgaa gaagtacttc atgacaatgc taatgctttt atgtcgaatg ttatgactct
    13741 tgtatctaat gacagttatc ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt
    13801 aactgctgca acattaaatc ttgggctaga taagaattta ggttatgcat atttagttcc
    13861 attcaatagt aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg
    13921 ctataaagga tatatccaat tagcccaacg atcaggtaaa tacaaagcat taaatgtgat
    13981 cgaagtttac gaaggagaac taaaaagctg gaaccgactg acagaagagt ttgagtttga
    14041 tccaaatggt agaacgtctg atgaagtcat tggatatgtt ggctatttcg agttactgaa
    14101 tggattcaaa aaaactgtct attggaccaa acaagaaatc gaagctcatc ggattgctaa
    14161 caataaagat cgcgataaga caaagttaag tggtgtgtgg gcatctgatt acaatgcaat
    14221 ggcacgaaaa actgttttga gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat
    14281 gcaagaagcc accacatcgg atgagagagt ccaaagggtt caagaagacg gcagcattat
    14341 tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa gaagcagagg ttatatctga
    14401 agaaaatgaa gatgtacaaa ctggattatt tgatgcatct aatccgccgt taaacaaata
    14461 atgagggagc tttctccctt gcttttctag aggagaaata cgaatgaaca caggatatgt
    14521 caaattacat cgaaaagtga tgaattcatt cgtttggacc aatccttata tgtataaatt
    14581 atggagcttg tgtttgatga aagcgagtca tgagaatcgc aagatacttt ttaatggaaa
    14641 agagatacaa gtgaacagcg gagaattcgt cacagggcgc gatgctatca catctgagat
    14701 gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga gatggctaaa
    14761 acaatttgaa aaaaacggaa tgttgaacat caaatcaact acgaaataca gcgttatatc
    14821 aataaaaaac tggtctttgt accaaagcag tgaacaacaa atgaacatca aacgcacaac
    14881 aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat tataagaatg aaaagaataa
    14941 taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact cagtttacta
    15001 cattctcgcg gaggagttat tcaaacagat ttgtcagaat caggaaatca aaaagccgaa
    15061 tctgcaaagt tgggcagata acattcggaa aatgatcgag atcgacaagc ggaccgaaaa
    15121 ccaggtacgg ggaatgattg aatggagtca gcacaatgtg ttttgggcat cgaatatttt
    15181 atctgctaaa aaattgcgag aaaaatacga cacaatggca gcgcaagcaa atcgtgatta
    15241 taaaacaaaa caaactaaaa cgcttgaata cgagaaattt agcacagatg agttgcctat
    15301 ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct gatacagaaa
    15361 gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg
    15421 aagaaccctg atggctcaga acgttgtaag ccaacctgta tgcaatgtgg gtataaagca
    15481 ttgcgagttc aggaagactt acaaacagaa cgaatttaca acgagagcct gaaagcaaga
    15541 gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca
    15601 ttgcagaatt atcaaattgt cgatcaagaa acaagacaag cggttgaagt aaccaaacgc
    15661 tttgttaatt cagtcttgtt aggaaatcca agtcaccttg ttttaactgg aaaacaagga
    15721 acaggtaaaa gtcacctagc gatggcagcg gcttgggaag tcttgaagcg atcaaactac
    15781 gataaaaaaa tcttatttat cgggttacag gaaatgctgg atcaaatcaa attttcgtac
    15841 aacaatcctg aactcagaaa aacgattgag ggatcgttga ttgcagatat caaaacagcg
    15901 gatttagtca tcattgatga tatcggttca gaactaggaa aagatgcatc agatagtcga
    15961 gcgtttggca taaacacgct aaattcgttc ttggatgcac gacagaacct agcaacgatt
    16021 atcacaacga acttgcttgg tgaagaactg aaaaaagctt acggtacgag aacaatatca
    16081 agaatgtttg tcaactctga tggatttacg atggtatttt ctcaaacagc agacaagcgc
    16141 ataaaaccag tgaaaggtag tatcgcatga ataaataccg taataaaaaa actgttcatc
    16201 gaggtatcaa gtttgattct atcgcggaag cagagtatta cgatctagcc ttgtggcaag
    16261 ctgaagcgaa tggttggaaa gtgaagctcc aggaaaggtt tgagctgatg ccgaagtttg
    16321 aactagaggg caagaagtat cgcaagatcg agtatattcc cgacttcaca ttttacaaaa
    16381 gcggcaagct ggtcaaagtc gtagatgtta aggggatgca gacaaaagac tttaagatca
    16441 aggcaaagtt gttctgtcat caatatcaag tgccgttgat attagccaaa aaatatcgga
    16501 atacgttcaa ggaagagcgt ttttaacgag gtggtccatc atgacaacag aagaagtgat
    16561 tcaaatgcga attcgaagcc ttcggcgtga gattgacgat ctggaacgaa caaaggcagt
    16621 gatggtcaat gaaacggcta gaaaggcaat cgatttgcac atagagaatt taagaaggga
    16681 aatccgtaga ttggaggaat gagcgtggat aaggaagcgg cttggcgaaa attaatgttg
    16741 ctgattcaag atgagaactg gcaagaagat gaagcagtgg ttgctgaagt tcagcgtcta
    16801 ggaaagattg ctaacggacg tatacgaaaa aagccagaca aaagaaaaca gcgcaaaggg
    16861 aaaatcgtcg ttgttttaca cgaaggcaaa attttgatgc aaggaacagc tagtgagctg
    16921 tctgcagaaa ctggatatac gcgtgggact attcgaacgt acgcttggcg aaatcacacc
    16981 gatcgaaaag ggcacgaata taagtatttg gaggaagaaa aatgaacgaa aacaaattaa
    17041 tcaaattggg tgtagcagga gcagtaatcg taggtattgg agttatcgga ggatttaagt
    17101 tcttcgaaaa aatcgataat ggatatgtgg gtgtgcgcta ttcaatgaac ggcggtatca
    17161 aagatgaagc actgacgcaa ggtgtgaaat ttgtagggat tgacaaagtg atccaatatc
    17221 caattcgctt gcaaactatc caatcaaaaa atatttcagt atctacaagc gacggcaaaa
    17281 agacaacgat tgatatcaaa tatgactaca aagttgattc aactaaagca gcaaaaatgt
    17341 acaaggaatt tgggaatatc acttcggaag atatcgaaag tggatggtta aaatctaagc
    17401 ttcaaaaggt cgctcgtgaa gtttatgcga aatatagtct gcttgatgtc ctttcaggag
    17461 attcctctaa agttgaagct gaggtattaa cgaactttgc taaatcagtt gaatctaaag
    17521 ggtttgaagt cgaagacgta acacttggtg ttccagatgt cgataaagaa acacaaaaat
    17581 caatcgatgc gatcattcga gctggtcaag aaaatgaaaa agcgaagcta gacgcagaaa
    17641 ctgcaaaaac tcaagctgat agtgaagctt acaagaaaac aaaagctgca gaagcagagg
    17701 cagaatctaa tcgcaaagtc gccgaatcag taacagacaa tttgattcgt tatgaagaag
    17761 ctcaagctcg caaaaagcat ggatgggtaa cagtaaatgg agcagatact gtagttacgg
    17821 atgaagcagg caaataatat gggattcttt atggctaaaa ttctcttgtt cttaggttta
    17881 gtaggagcag catatctcgt gtatgcgctc ctttcccaaa ctgatgacaa agaagatgac
    17941 aacaacgatg aaatgaaatt ttaggaggag aaataatgga cgaactaatc acaaaagtag
    18001 agcagtgggc taaagacaag ggactggatc aagcagatcc aaaagcacag tttttgaaag
    18061 tagctgagga attcggggaa atagcttcgg cgatggcaag aagtaatgat gaactattta
    18121 aagatagcgt aggagacgtt atcgtcacgc tgattatcct ttccatgcaa aaagggacaa
    18181 acgtacaaga gtgtttagaa atggcataca acgaaatcaa aggacgcaca gggaaaatgg
    18241 tagatggtgt attcgtgaag tcgagtgatt tggaggatgt gaaatgaaat acgaaatacc
    18301 actaagtgaa gcgggcattc aagcaattat caatggtcgg gaggttaaca tagaacttcc
    18361 tgatggtact gaattagtca tcagacaaag ttatttgaaa gatatggcag ctccagtatt
    18421 aattgatcgt tttaacgtga ctgattctgt ggtagagaac cacttaaaag aatttcgatc
    18481 aagtatagac aacactttca gattagggag ttgattgaca atgaacacca gacaccgcag
    18541 agtagcaaaa ctaagaaaac aggaactaaa tgtactaaag acaaagtttg aaaaagaata
    18601 tggaatttca gcagaagaaa catataaagt ggcaagtcag tttgttgctg atgcaagtga
    18661 tgctattcgt aagtttggga tttcgatatt aaatgatgat cgtaaatggg aggaaatgag
    18721 atgaaactaa aagacggatt ttacgctagt agtcacggca tcggcggttt aatgctagat
    18781 atgccgacaa agaaccctaa aacacgtaag aaaccaaaag tcaaagtcgg tgacatggtt
    18841 cgctgtgaag cagaagagtt catttatccg tttcgtggat atgtagaaca cgtctataat
    18901 cactcagcga tcattcgtat tgaaaacaca atggaatgcg ataagtggtt agcgaaaagc
    18961 aaagagaatt tagctgtagc gagattggtg gatatggagg ttataaacaa tgaagtttaa
    19021 aatctttgaa gaggacactc gttataaatt agaaaaagaa ttaaacgatt ttgcgaaaaa
    19081 caatgagatt cagcatatat ctttagcaac ttctaagaga ggttatgcaa attactatgc
    19141 agctgttgtg agctatgtaa gtcgagaagt gtaactcggc aaataaaaaa agccggatcg
    19201 ctccgactga ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact
    19261 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
    19321 tgaagaactt tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
    19381 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
    19441 ttcaaatgat ggatatagaa gcggagagag acgcgattct agtggctttg atggctctta
    19501 gtctgattag tcgtcagata ctctactaca gcttctgtgt gccagatagc ttctcaaact
    19561 acaggattag ccgtgaagtg ggttattcag aaagaagcat acaaaggatg aagtcggaag
    19621 ctctaataga gtttgcagaa gcgtataaac acggaagaat aattgcttat aaataggggg
    19681 accatataat gtggaaagat tatgtatcgt tgaaagagtt gaaaaaagat cttgttttca
    19741 aaagaattgt agaatggtca gagagtgaat taattttaga agatggaacc aaaatggaag
    19801 ttgtatgtag cgaatcagat tgctgcgcat gggccgaagg tgaattcaaa aatgtaaagc
    19861 ttgatgcagt gattacagat ataaagattt ttgataaagg taaccgtctc tataatggtg
    19921 acggacatac ctcttatgct gaagtcgttg tttatcataa tcgtaatgag atcgctaaag
    19981 cagaatgcac agcgaatgat gggaatggcg gctactatta tagtgtttgt gctttaaagg
    20041 tcaaagacaa actttgtata gtgactgacg cataaaaaaa tggcggtttt ttggcggaat
    20101 gatggcggtt tttagccatt tatcagtgat attatggtag tgtcgaaaga ttagtgatag
    20161 gtctgagaca aaataataat aaaaggaaca tcgttttatt attgtttcac aattaagctt
    20221 cgatagacag caacggaaat attaagaata aggatgtgaa ttttaactcc ttctaaattg
    20281 ttcttattat ctatcatccg ttgctgtcta ttgttacata cagatcgctt aggcggtctt
    20341 tttattttgg ccgaaaacct acattttcga tggccgatta ttggaggagg aatagcatgt
    20401 tcaaactatc tgaaatcatt aagaagtccg acgttgagaa attagagatg ttaaaaaaga
    20461 agttgaagaa atagcagtct cttcgtgagg ctgttttatt ttgctcacaa aaatagacca
    20521 ctatcgggta atagtggtca ggaattaaat gaaaaagatg ttagggttgt tagctaagta
    20581 taacatcata acgcttacaa aacaatgcaa gaaggataaa ttattatgag aacttactgg
    20641 tatgtgtcat taaacaataa atatccgaag ccaatgaaag gacagcatag gcgtgtagtc
    20701 atgtctgttc aaatgaaggc gaagtattcg attgtagaaa tgatcagaga ggcaacgcca
    20761 gtagagattg attattgcaa gctagtctat tgtgggtgcg gccgttggaa agaggatcat
    20821 gtacaaaaaa atatcagcaa gtacatatag agaggcggtg attaaggatg gaaaaaagcg
    20881 atacgaaagc gaacagatac aaacctactg ctgcagagaa aaaactcttg gaagtattga
    20941 taaatccaga taacttaggc aaaccggtgc aagagttgtg ttcactggcg aaaataggta
    21001 ggaccaaata ttacgacgcc atgagcaaag atggctttgt tgatctagtt aacgaaacaa
    21061 caatggatct gatcaaagga aaagcttctg atgtccttaa tgccacttac aagtatgcat
    21121 tgactgaaaa aggtcatcag gataggaaaa tgattctgac tattgctgga atctatgcag
    21181 acaaacaaga actcaaacat tctggcggcg tcgatatccg aaaacagtat gagaaaatgt
    21241 ccgatgaaga attagaagag ttggtgaaac ggtatgaaaa aatcaacgac tcttaatcgt
    21301 gttgagtatg ccgttgttca aagagagcta gccattcgaa aagctaggaa aagtttttgg
    21361 ctattctgcc agcttcttta tccagagttt tacaaagatg atcggttgta tttgaaagat
    21421 ttatgtcata cgctacaaga tttttacgaa gataaaatcg acaaaaaaat tttggttatt
    21481 aacatgccgc ctcgacatgg gaagactttt acagcgcggc tattcgtttt atggatgttt
    21541 ggacaaaatc caaagacgaa aattattacc ggatcttata accaaatcct gtcatcttta
    21601 tttgcgcaac aaacacgtga tggaatcatg gttgaaaacg aaaatgttaa ggctgaatac
    21661 tttaaagata tttttccaga ggtgcaaatc aaatatggag atgctgcaaa gggtttctgg
    21721 tctttagatg gttcagaaga aaagaattat ttagcgactt ctcctggtgg aacatccact
    21781 ggtattggcg cgaattatgt aattgttgat gacatcatta aaaccgcaga agaagcctat
    21841 aacgagcgtg ttttagattc tcactgggaa tggtataaca acacgttggc ccaacgaatg
    21901 gaacggccac gtaaacagat attgattatg acacgttggt caagtaatga tttagccgga
    21961 aaaatgttga cgagacgaaa aaataatgtt catcagatct gttataaagc agtacaagaa
    22021 gacggctcga tgttgtgcaa cgagattatg acccacgatg agtacttaga tgtggtacaa
    22081 gaaatgaatg tagatattgc agaagccaac tatcagcaag aaccaattga ccaaaaaggc
    22141 aggctatatc agaagtttct tacttatgat acgttgcctg ataacattat caaaatatgg
    22201 aactacaccg acaccgccga caagggtgcc gattactttg cgtctcctgt ttttgctgag
    22261 acgtctgatc atgatgctta tattatcgat gtcatgtata caaaagagcc gatggaaaca
    22321 actgaaatag cccacgctaa tatgattatt cgcaatcgag ttaataaggt acggattgaa
    22381 ggaaacaatg gcgggcgtgg attcagaaga aattcagaaa gaattacaaa agagcgaggc
    22441 tatcgaggtg cagtatttga agactttcat caatcggata ataaactgtc tagaatactg
    22501 gcgaatagtg cttgggtgga aaatcatgtt tactatccag atgattgggc gacacgttgg
    22561 ccggactttt atgaggctat ggttagttat caacgtgaag gtaagaacgc tcatgatgat
    22621 gctccggatg cagttacagg aattgcagaa acgattatag aagcagatga cttcgagttt
    22681 attgcttatt aggaggtggg tctttgtttt attctggaat cacattgaaa aaatacaaac
    22741 gtttaagaac gaaatattca acgcaaatcg aagaaggatt attcgatcct aacgctttta
    22801 tggaagatat gaaacccttt ttcgatgatc gagaaagaaa gtatcttgct tatactagcg
    22861 agaaaaacga gatcgacagt cgtgagaaac caaaaacaaa aatcattaag gtcaataaca
    22921 agattcatgc gggaatgtat tcagtggtcg ttgatcaagc agttaaccat ttcacaggaa
    22981 taccaattaa atgggattat gatgtttcgg aacagaaaag gacattcatt caacgaatga
    23041 gagacaaatt actgggtaat gatatcagcg ccccttctac cccagaagaa tttgaaacac
    23101 tcacgagaca attaagtact atgcggtttg gaatgttaga ccccgatact gcaacatttc
    23161 aaggagcgac agggcttgct ttcagattac tggagcctgt tgagacaaat gatggttggg
    23221 aactaagagc aagcaacatt gagccgtgga aagctgaaaa gtacgaaaac ggcgcagtgt
    23281 acattaaaga gaaatacgat ccatatcaaa agaagtttta tgaagagatg aaggttgtca
    23341 cgccgaaatc aatatttact tatgctcgat atactgatta ccctggcacg gtacaaggag
    23401 catttaaaac gctgggtaat gttcctaatc ctttaggcgt tatcaatctt acggagttta
    23461 aaaacaatac aaaccgttac tgtgactttg aagttgcaga ggagattagt gatgctattg
    23521 atcgtgtttt atctgatcag cagaacgaga ttgaacaatt caagttagct tatatgctga
    23581 tcactggggc agatttgtcc gaggataaag ccaagaagat gatggagcaa ctagggatca
    23641 tcaatctgaa agaccctaat gcgaaagccg gctacgtgac aaaggatcta caaaaggatt
    23701 tcaacgagta tcattttgat cttcttaaaa agcaattcta cacggtgtgt aaagcaattg
    23761 atttcaatga cgaagtcttt aagtcgaata gttctgggga agcccgaaag tggcaaatca
    23821 ttagtttaga agctaagaca aataccaaag aacagtactt tcgagaagga ttgaaagagt
    23881 gtgccgaggt tatggctgct tatttggatt ttgccgagaa agttacaatt gatccggaaa
    23941 agatcatttt caccttctct cgttcgcttc caacagattt aagctaccta gctgaggcat
    24001 tgcctaagtt ggcaccatac gtttctaaga ggacgattca aagtcagatc ccgttcgtca
    24061 cagatgttga ttatgaaaac gaaatgattg acgtcgaaag cagacaatct tacccagacg
    24121 gcgagtatgg aggtggcatc gttgagtcaa atagaaacga agtactggac caaacgtcga
    24181 gaactggaag atttagcaag actcaaaatg gaaaatcaaa cgctaaagaa actaagtagt
    24241 gtctttcctg atgcgttgaa agaaattcaa gcggagttat tatcccaagc tgatttacac
    24301 gatattacct atgctgagat gatggaattt tatagtacaa gtgatcagaa aaaatatcgt
    24361 gaatacgttg aaaagaacta caaatcactg aagatgtatg atgcaaaata caaagagttc
    24421 atcgaagaat actttccttc tttcgactac gccaaagtga atcggctgct tcaaatgagg
    24481 tcagatattt ttcgaattct tgcagagagt gcaatagatg cagatgtgaa taaatatttt
    24541 tcggatcggt tagaagatat cttgcaacgt tcgtatgtat caaacgcaaa cgtatttgcc
    24601 cagattttga acgtagattt gccgaattac ttatcaaagg aagaacttga acgttacctg
    24661 aactatcctt ggtcaggtaa gacattctcg cgtcgtttat ggggcaatat ttcatctcta
    24721 gaacaaaagc tatctaacgc cattacaact agtgtagcaa gtggcgaagg agttctcgtg
    24781 gcattacgaa caatgcgaga agacccagaa atatgcgata tgttcaaaag ggaggaatcg
    24841 aagtacaaca aggcgatcga aaatcttgtg agaacagaat acgctaaatt cgctcaagat
    24901 ggtattgaga aatcttatat tgaaactggc gtagacgaat acaatgtctt aactgcaaaa
    24961 gatgaaaagg tatgttcaat atgcggtggc aaagcaaaga acaacccata caagttgagt
    25021 gaagcgatca ttggtgagaa ccgtgcgcct tttcatggcc gatgcagatg tacagatgtt
    25081 cctaatatgc cgaaattagg aaaagatatt gacgaagagt atgagcgttt atttggcgat
    25141 ttattagatg agtttgcaca tgattctttt ggaattaatt tgaaacggag gaagtagaat
    25201 gaaagatttt tttgaagcag tactaacaat taatgtaaat gctgatattg cagaagccta
    25261 caaaacagct attgagtctg agaaccatcc taatggcttg agagaccatt ggaatggcaa
    25321 ttatgcctac gtggttattg gcgatcaaac tgttaattat caagataata ctccagttga
    25381 taagaatacc gttaatttaa cgattcaatt attatctcat tcattaccaa atttaaaaga
    25441 aacagttgat tggtatgaaa atatgggatg tattgttgtt agaactgact acaaagaagg
    25501 aaagtctagt aattaggctt ttttattttg tccgaaatga cgttaaacta gcgcaatact
    25561 gggcttggtt gaatggtggg gcgcaactat taaaactcaa agcaatgcgg ggcgtgaaaa
    25621 cgaatcgtgg ggcgaaagga gaatgattat gaaacacaaa tcattaatgc caatgaattt
    25681 gcaatacttt gccgaaggtg acgatcaaaa gttttctttt gatgacttca aatcttttgt
    25741 ggaatccaat gaagaagctc aaaaatttat tcagtcacaa tctcaaagtg ttgccgacaa
    25801 acaattggaa gcttggaaac aaaataactt agataaaatt aaacaggata ccatcaagga
    25861 atatgaggaa tctaagaaaa acaagtcacc tgagcaaatt caactggaaa aactacaagc
    25921 tgaatttgaa gcagaaaaag cgttgcgtgt gacaagtgat aataaagcat ttgttgcaga
    25981 acaaattgct ggattagaac tagatggaga gttaaaagag tctatttctc aatttatgct
    26041 aaataatctt gttagttcgg atacagattt cactaagaat gctgttgaag gttttacaag
    26101 tgttttgaat gcaattaatg agaaacatgc agatgcatta aaagaactac aaatgaagtc
    26161 tgcatttggt ggaactcaac aatcgaataa ccaggttcag cagaacaatg aaacattaac
    26221 aaatccagaa gaacaattag gacaaattct tcaacaattt aactaggaga gtgaaaaatt
    26281 atgaaaaaaa catctttaaa taatttagag tatttggata tttcaccagc gattaatgct
    26341 atgcaagtac caaatacacc ttttttaagc tatttacttg gtgctggaaa aacagagcca
    26401 gcaaactcga cagaaattaa atggcgtgaa tatgatatca acaacgatga ttcttctgaa
    26461 aaacttgagg gcggagaata tccagatgct gaatcaggtc gaacttggtt taacaactat
    26521 actgaaattt ttagaaaatc aacctctgta tctggtacat tagatgctat taatgtgaat
    26581 ggagtcggaa atgaattaac taatcaagta gcactacgtg gtatggaaat gaaaattgat
    26641 ttgaaccgaa aattgattac tggtgtaaaa gctgatgaaa atggttctaa aggtcgtcga
    26701 atgaatggga ttttgaactt gatcaattca gcaaataagg cagaaacagc cactgcgggt
    26761 gcagtaacaa gaaaagatat cgatacttta tttaaattga tgtatgaaaa aggttatatg
    26821 ggagaaaaac tatgcttgat ttctccagat atgcaggagt taatgactga tgagttagat
    26881 ggaaaatcaa caaaaattgt tcagttcggg gaaagagtaa cttttggatt gcaaattgga
    26941 aatatcgtgt ctaattacgg tacaggtatt gctctactag aaccatcatt gccaaaagga
    27001 acaattgccg caatcgatac taattatgtg aaactacgtc cattacgtga atggagagca
    27061 gaagaacttg caaaaacaac ggattctaga cgtatcggtc ttgtaggtga atactctctt
    27121 gaatacaatg cttcaaactc tggggcaatt ttaaatttaa agtctgaata aaagggagtt
    27181 agtactccct tttttgatag gaggaattac agtggtaaaa aaagatgaaa ctaaaaaaga
    27241 tgaagtcgtg aaatatagag taggtaaaac taaaaatttt gttgggtttg ttcatcctaa
    27301 aactcgtaga tttatcacag cagattcaaa taacgaattt atcatttcta tagatgataa
    27361 agaagcaatt gcaattttgg aagatgcaat tgatgttaat gaaatttagg aagtgatctg
    27421 atggatgaat cgctaaaaac ggaaatcatt gagtctacaa aagaagattt tccagatttg
    27481 agtgaagaac gcataactaa tttattagaa ataattttgc tagaaattga atcatacaac
    27541 acttgtaaaa atgatatttc atgggaaaag ttaaaaagcg tgattagtga agtgttgtat
    27601 aaaataataa aaaatgaatc agaaaaaaca gtatcttcag ttagacgtgg cgatacaacg
    27661 attagttatg cttcaacaac aaatgatgtt agtgagttac ttctaggcta tggtgatttg
    27721 atacgaagag ttattggttg tggaggattg gagttttttt aatgaatgaa gcagatattt
    27781 tagaaactac ttatgaagat agttgtatta ttgaaagact gacggacatt gaagattcta
    27841 atacaaatat tactattcaa gattacaaaa aagtatatga taatcctatt tcttgcgctc
    27901 tttcacaagg tcaaatcgat ggactagcag tcatagaaga tggagagatg gtaaatgttt
    27961 caactgacac atataaatta tttgttcatc ctaagattaa actaaaaaaa ggagatcgaa
    28021 taacaataac tcaaaaggcc agtggcttaa ttttttctct atttgctact aagccttttt
    28081 actatcctag tcattgtgaa gtgaatttga taggaagtga gaaaaatggg taatctcaaa
    28141 tatgaatcaa atgcagaaaa gatcattgag aattttaaaa atatgactgt aattgctcaa
    28201 aaagaaggaa tatccttcgt taacgattca atgaataaag tcgttagttt agctaaacca
    28261 ttaactcctg taaaatcagg taatttaagg cgaggatatc gtgtagtaaa agctagaaaa
    28321 ctatcaagcg gtcgaattgt tggagcagtt ataaacaatg aacattattt taaatatgta
    28381 gaagaaggtc ataggactaa aaatggtggc tttgtcaaag ggaaattcat gttaactcgt
    28441 gcaacgaatc ttgcaaatat gtcttatatt cctcgaagat ttaaacaaat ggcaataaaa
    28501 atcgttaaga aaggaaagta acatgaaaga tgaaatcatt gctgcaatca gtagcaaatt
    28561 aaaagaaatc tatcctgatg ggacaatata tcttgattca gttatgcagt caactaaaga
    28621 tttttatttc gttttatcag taatggaatc tggaactgaa aatgtaggaa ttgatattca
    28681 aaatgtttct ttcttaattg atattgcatt gattgataat aaacctaata gaaatttaat
    28741 taatgaatta gtctcacgtt gtgggacttt ttttaatacg attacaatcg atgagcaaat
    28801 attatttcca aaagcctatt tacctgatga agcagatggt gttcaacata ttcgttttac
    28861 attagaattt ccacaatata ttgaatggag tgaaagataa atgggagaaa aaagaagtaa
    28921 aaccggaatt atttctgtcg aaaaacctac ttggtttcct ttggaagatg agacagggaa
    28981 tttcccggta tatggtactg cgttgccaat gggaacagca gtaagtatca aaccaacagc
    29041 taattatgaa acaacacaag attatgggga ttcagtggtt caagatcaat ttacagcttt
    29101 tggtggtgca gaagttgaac tggaagcaaa tggatatact cataaagttt tatctacaat
    29161 tactggaggg aaaattgtta aaggtggtgc cttgcgttct ggagaagata ttgcgcaaga
    29221 tggtgcgttt gcatatagac gtaaaaaatc gaatgggaaa taccgttata cggtctttta
    29281 taaaggacag tttgctctag attcagatga aacgtcaacg attgaaggaa gtaaagtaag
    29341 ttttactcat ccaacttgga agggttcatt tgtagatgta ccaggacttg gatatatgta
    29401 ttcagttgat gaagatgatg aaggcgtgga taaagcaatg atcggaaatt ggtttacaaa
    29461 agtagcaatt ccaattgaag aagcagaact ttcaggaggt acagaataat gtcaaaatat
    29521 caaacaacga ttaaattaac gaaaaaagat gaagaaggta aatatgagca agtacaattt
    29581 aaatctgctg aatttttacc aggaacagtt gtagaagatg ctgcaggagt tatggaagaa
    29641 atgcaaacag cgactgataa acaatcagtt aaaaaagctt taagtcgtgc ttattcattt
    29701 attgcagata ctttatttga gggacaattt actggtgaag attattgcaa aggaattgat
    29761 gctcgtgaga ttgcctcatt gacaggaaaa ttattgaagt ctgttactgc aggttttgat
    29821 gaaacttata cagagacgaa aaaaaagtaa gtgaggcgct caagtcacct tcatttaagt
    29881 attcgattac ttaccgagaa ttagatataa agacgcaatt actcgaagca ggttggacgt
    29941 taccagaaat tgaacgtact gacttggatg agttaatgcg tctttttgct tttagagatg
    30001 cggtaaaaga gcatgaagat gttgagtact atgataattt cactcaattt taggaggtga
    30061 tactttgaat aacgatgacc taattctgaa gatgatacta gatgaatctg gttttactgc
    30121 cggtatgaat aatgctgtca aaaagttgaa ctcttttgat gaaacaatcg aaagaaaaag
    30181 tagaaatagt ggcagttcat taggtagtat ttggaaaata tttgctggaa gttttctagc
    30241 tagcggagta actagaatcg taggagctgg ctttgatcta atcaagggtt ccataagtgg
    30301 agcggttgat cgagtagata cgatgaataa cgctctacga aatttccaaa atatgggatt
    30361 tagtaattca gaaattatga agaatatcgg aaagaatggg cttttatctc aaggtattca
    30421 aggacttcct actgctttga atgatgcgat aagtcatgtt caacttcttg cttcttctac
    30481 aggtgattta actcgttcaa ctcaaatatt taaagcttta aatgatggaa ttcttggttt
    30541 cggtggttca actgatcaag ttaatgaagc tgttattcaa ttatctcaaa gtttctcaaa
    30601 cggaaaagta gacgcacaaa cttggaactc aatgatcaac gctcaacttg gacctactct
    30661 ttctgctatt gctaaaaaga tgggaattac aatgggagat ctgaaagaag gtttatctca
    30721 aggtaagatt tctgttgaag aattccaaaa tcaattaata gaaatggata ccaaaggtgg
    30781 cggaggactt aaatcattaa gtcaaatcgc taaagattct actaagggaa ttaaaacctc
    30841 tatacaaaat gcaaaaacag ctgttacacg tggtgttggt gaggtaatag aaggattaaa
    30901 taaagcatta gtagattcag atttaggtgg atttaaggga attattgata aagtgggcag
    30961 ttcaatggaa tcgttcttaa aagtaattgc tgcaaatatt cctacagcag tatcattttt
    31021 aagtaattta tttaacgaaa ttcaaaaatt tggttctgct ttgaaattca tgatgccctt
    31081 tcttgttcct gcagctaccg cttttggagc gttcatgttt caacttaaag gtataccagc
    31141 aataataaaa agcttcaata attttaagaa tgccataatt ggcgttggaa attcattaaa
    31201 gattatgggg gcaatagctg ctgctaattc atttgtttta attattggtg cagtcgttgg
    31261 tgcaatagct gttttcggtt actttatggc aaccaatgaa gagtttagaa acaaggttat
    31321 atctatttgg aatgatgtga aagattccgt aattggtgta ttaaaaaata taaaggactg
    31381 gggaattgat acttggaatt ctgctaaaga aatggcatca aatgctgttg agagtgtcaa
    31441 ggatgcttgg tctggaataa aggaatggtt ttcaaatact tggcaaggca ttaaaaatgg
    31501 agcaacaggt ttatttgata aaacagtaga aacatctatg aatgcggttg atagtgtaaa
    31561 aaacgcatgg tcaaatacaa agcaatggtt ttctgatatt tggcagagca taaaagattc
    31621 agcaactgaa aaatggaatg aaattaaagg ttctatcatg gaagttgctg gtccattgat
    31681 tacaggttta aagaacgcat tcttgcatgt cactttttat ttagagacct tatggaataa
    31741 tctagttgag attggtaaaa atgtttttga gattttaaaa aatgttatac ttacgccagt
    31801 tttgtttatt acctccatga tttctggtgg atgggaagaa acaaaaaaca acatgattgg
    31861 tgtatggaat aatattaaag aaagtgctct aaatatttgg gagtctataa aaaatatttt
    31921 tgttagttac attacaaata tttattttgc tgcgcttaat atttggacag ggttcaaact
    31981 tactttgata aacatttgga atgaagtagt aagtcaagct aaatcaattt ggattaatgt
    32041 aaaatacttt tttattaatc tttggattga cattaagtat tttgctattc aaaaatggat
    32101 tgaattaaaa ttcgggatta ttcaaacttg gattgattta aaatataatg ctattactac
    32161 ttggaacaat attaaacagt tcttcaaaga tacttggcag aacattaaag atatagcata
    32221 caatacatgg atttctataa agaattccat gatcaatact tggaataaca tcaaggaatc
    32281 tttctggaat attgttactg gaattgttaa ctccgctgaa aatgcatgga ctaatctaaa
    32341 aaatggtgtt tcaaaagcaa ttaatcgggt gaaagaaatc tttgattcat taagggaaat
    32401 caatttattc gaaatcggta agaatatcat tgatggactt atcaatggtg tagtagaaaa
    32461 atggaatgca ttgaaaaaga ctattaaagg aatagctgga agtatcaaag attctattaa
    32521 gggtgcatta ggtattcatt ctccatctag atggatgagg gatatggtag gtaaaaatat
    32581 tgttcaaggt attatcgttg gtattgataa agaaaaaagt aaattggatc agacaatgac
    32641 tgatttagtg aaaactccgt cggttcaacc agttattaca gggtctaata gtcaaccagt
    32701 ggtacaagct aaacaaaata catcttcaaa tgcagtgaat gaaattcact tgcatttaaa
    32761 tgtttatgga gatctacctg attcgatgat taaacaaatc gccaagaaga tgaaaacaga
    32821 attgacgaga caaatgaaac gagatgctga tgcagtagga gggacattat atgcaactta
    32881 aaagaggaca attttttatc aatcaacatt attcttctga gtttaacgta tatattcaaa
    32941 ataggcctgc ctctgtttca gctagtcgtg taattgaatt gagagaacgt gaaggcaatg
    33001 actcaattat tatcgataaa gcctattaca aaaacgttac tagaaaaatt gaatgttatt
    33061 acaaagcacc atcgattgat ttagtgcagg aatgggaaga tcgaattact gaatggttag
    33121 atatgaactc ttacagtgat tttattcttt attacgatca acaatatatc tatcaagcta
    33181 tagtaaccga agcgccagaa tttaaaggaa caagaaaaac agggaacata gttccatttg
    33241 aatttacagt tagtcttcga ccattcaaag aaaattatag tggtcgtttt gctattcaac
    33301 agataaaagc tttcgagtta tttaatccag agaagtatgc ttcaaaaccg cttattaaat
    33361 tgagtggttc tggagatgct tctttttata ttaataatga taaatatgat ttgaaactat
    33421 tagatagaga attatatata gattctaaac tagaagagtc ttatcgaaag ctagatgaca
    33481 atttagaaca tcaagatcat gtcactttat ttttagattt tccatttcta taccctggaa
    33541 aaaatgaaat caaatggacg aataacatac attcgtttga gataatacca aggtggtgga
    33601 gaaaagtatg ataccaagaa tatatagtcc taccgagaca gatttttcta cgaatggttt
    33661 agggattttg aaggacacca caaaatgtga gatatatgaa gtagcaaatg gaaaatatga
    33721 attagagttg gagtatccat taggtactcg atttgatgaa tattttgaaa atgactatca
    33781 aataaaagca aagtcaaatg atcaagaaga gtatcatatc ttttttattg atgataaaga
    33841 tattgatacc tttttagata ccgtaactat ttatgcccag agtcgtacaa atcggcttgg
    33901 aagacgagcg gtcacttttg ctggagtcga ctctaaaact ggtcgagaag caatggcaat
    33961 tattgaaaac aatatggata aaaaatcaga tatacgctta tattctgata ttacaactgt
    34021 atcaagtaca acttttgaag caagaaatgt tttaaattgt attgctggtg aacaaggatc
    34081 tttacttcag tattggggcg gagaaattaa acgtgagcct tttaagctat ctttattaaa
    34141 gcgaagaggt cgtgataata ttggcacaat ccgatatgga aaagatttat ctgggttaaa
    34201 agttaagtta gattggacag gcgtgaaaac aagaattatt ccgtatgctg atcctcaaag
    34261 tgatgcaggt acaactagcc gaatttatgg ctcgccagta gatagcgaat atattaataa
    34321 ttatcctgat gtatatacag aacacgttca attcacagaa gaacagggag ttaaagacgt
    34381 taatagctta aataaaatag ctaagaatta tttcaaaaca attaatccag gctgtgataa
    34441 accgaagatt tctattaccg ttgagttcga taaattaacg gacaccgaag aaggaaaaga
    34501 attcgctaag ataagaaatt atgggttatt tgatacgttt aaaatatatc atcgaaaata
    34561 taaactatat tttgaatcga aagttagcgg tgttcaatat gattcattat ctgaaaaggt
    34621 tttgaaattg gaagctgggg atgctcaagt tgctttttat caacaacaag ctgttactat
    34681 tcaagataaa ttaaaagatt atgctacgaa taattatatg agtagtttta atgattatgt
    34741 gtcctctatg attgcaggtc aaggaaatgc aggcggttat gtagttttat ggccaaaaga
    34801 aaaaccttca aatattttta taatggatag ccctgattta aacaaggcaa aagaagtatt
    34861 aagaatgaac aaaaatggaa ttgccttttc taaaaatggt tggaatggtc cttttaattc
    34921 cgcttggaca ttggatagta tattcaatgc taattttatt caaacaggat taataaaggc
    34981 agatatcttt caaaactctt ttaataaaac tggagatgta ttgaaattag ttaatggact
    35041 acttcaaatt tggaataaca aaaagaaaat aatggagtta accaaaaaag gaatggagtt
    35101 ttggaattct aatagttcaa ttggaacgat tggaacaact gattctgctg gtaatccttt
    35161 tcctggagct tctactccca cacctattga agataattct ttagttattc gtacaaatgg
    35221 agacggcaaa tatattttga tttctcctaa agttggtaaa ggattagttt tattaggaaa
    35281 tggtaaagca atttattttg gagacttaga tgtacaaggc aaactcacag ttaacggaaa
    35341 agaaatcaca gggaacaaca gtggaggaag tgatcctgga actatccctc ctcaattgac
    35401 gacggaagct gaaaaaagag catggaaaat ttggacaatg ctgaaagctc gtggctattc
    35461 tgaatatgca gctgcaggta ttctaggaaa tattcagggg gaggttggag caagtatgaa
    35521 ccctgataca gaacaacttg gtggtccagc ttatgggatt gttcaatggg atggttctgc
    35581 ttatccgtta gtcggatcac caacttggaa cggacgagag tatgttcagc gtttgatgaa
    35641 caccgcaggg attcaagaag attatcgaag tattgaagcc caagtaaaat tattagattg
    35701 gtgtatgttc aacggtcaat ggctcggaaa agtaaatcca accacagtat caggatttaa
    35761 atcgatcaat gatgccaaaa gtgcagcgta tgcttttgaa atgaacttcg aacgcccagc
    35821 ttctgcacat ccagaacgcc aaaattatgc ccaatcttgg tataacaaat tacatggatt
    35881 aactagtcca gaacctggag ggaatttcat ttgtccaatt caaaaaccag tgacagttac
    35941 ttcagaatgt ggatggagaa ctagtccaat aaatggcggc caagaatttc ataatggaat
    36001 tgatcttgta aatggaaatc ctaatacacc tgtttttgca gcattagatg gggaagttgt
    36061 tcaggctggt gctaattatt atgactggta tggtaattac gtggttatta aacataataa
    36121 tgggaagtgg acaggttatg ctcatttgtc tcgtattgat gtttctgttg gacaaaaagt
    36181 ccagaaaggt gctcaaatag gcttgatggg aacaactggt ccatctacag gagaacatct
    36241 acattttcaa attatgaaaa attattggcc acagccagtt gttgattttg agaatccaag
    36301 aaattatatc caattttaag gtggtgattc tatgagtaaa tggaatgtcg ttttaagtac
    36361 aacagaacca tataattatg tggggatgat tcaagttcga caaggcaata agaatacaga
    36421 ggttatggaa gcgactatag ttgaaaatgg tcttccctac gatttatcag aatgtaaggt
    36481 atattttgaa tcagttgtag gtgggaaata tccagtccaa ttagaaacaa aaattgtgga
    36541 tgctaaaaaa gggaaaatta actatatttt tgataaatat tccatgcagt gtttgcatcg
    36601 acaaacagcc aatttcatta tatttaaagg agaagacttg attggaacaa ctcaagactt
    36661 ctcctatttt gtcatcaatg ctgtttcaaa aacagaagga gaaatgggtt cttattggca
    36721 atcaattgaa gatttaattg ctgatatgac agattttatt aatgaaaata aaggcgattt
    36781 tacggactgg atgaatgaaa ggaaagaaga atttgatcgc tggagagaag aacaggagaa
    36841 tagtttccaa gattggagag aaggacaaga atcagattat ttgtcatggt ttgaatcaat
    36901 caaggatatt ttgaagtcta tcgatccagg tggagtaatg ttagccgaat taatggatgc
    36961 acgtgttgat attcaaggag ttcgtcatgc gtctatttca gagcgcttgc tggcagatat
    37021 ggattatcta tatcagaaat tacgagcaac acttttcacg attgaatacg gtgagattga
    37081 agtgactgat attttgcagg atgatctctt ttcagataat cacgaagtcg aaaaagttgg
    37141 aactgtagaa tttccaattg aagaaggagc tttgattatt gcaactgttg atgatccaaa
    37201 acagaatgtt ttcactcttg agaaagttgg ggtgatttga tggttaaagt aaagagaatg
    37261 atggagactg atgaatctgg tgttaaacgt caatttatgc caattacaca tgtttctgca
    37321 gttttaggct tagaaaaaat gatatcagga caatcaaaag ttctttcggt taatggtaaa
    37381 tatggtgcag tcattttaac gaaagctgat ttaggattag agaatgcaat tactgaacta
    37441 ccttatgcaa gcgagacaag cgatggtatt cttactgctg aaatgtttca aaagatcgta
    37501 aatggagaag gtggaacata tattttgccg attgctactc ctgaacaatt aggaggtata
    37561 aaagttgggg aactcttaga agttactgaa gaaggtgttt tatctgcaac taagcaaaca
    37621 gacttcaatt tctctgaaga attaaaggga aaattagaat ctctaaaaat attaaaagca
    37681 ggtgcaaata tatcaattgc agaagatgga acaattagtt ctacaggtgg tagtgggact
    37741 ggtggtgtta atcagtcata tgtagatcaa aaattccaag aagctgtaaa tcaagcagaa
    37801 aattacacaa acgaaagaat tccaaatttt acttttgaaa aaatcgggga ggtatagaaa
    37861 atgacagata tcgtaaaaat aaaacaaagc ggtgtacagg tttatccaca aactcattgg
    37921 aatgctatag aaggcaaacc aacaacggtg aagggagata aaggcgatcc tggccaagca
    37981 gcaacaatta ccataggaac cgtttctagt ggttcaaccg cttctgttac gaatgtagga
    38041 acatcatctg cagctagatt taattttgtt ttaccaaagg gagataaagg tgatcctgga
    38101 ataaatgcca caactacagc tgtagctaca actacagcaa atgggttgat gtcgtccaca
    38161 gataaaacta agttggatgg aattgcagct ggagcacaga aaaatccagg taatgctaca
    38221 actacgacag caggtttaat gtcagcaacc gataaagtga aacttgatgg attagcgaat
    38281 attacatttg agaaagtggg gacggtttaa tggctgatat tgtgcaatta aaagaagacg
    38341 gagttgctaa atacttaaaa acgcatgtag aagctattga tggtaaagaa gcattggtgc
    38401 agacagatgg agaccaagcc attgcaggac ataagaattt ttcaggttct gtaacaatca
    38461 ataataaacg tgttttgacg actgatgata acagatatga agtggtaaat ctggttgtca
    38521 ctaacggtaa tacagggaca gcaaagcttt atcgtgaagg aaaaacagtt tctatatatt
    38581 ttgtggcttt aaacggaaaa agtagtggcg ggaatgattc ggttatttta actgttccag
    38641 aaggctatcg gacaccaatt agttttgaac aactggttgg gtcaatagac cgttctactt
    38701 tgaacagtgc tcaactatct attggcgcag acggagccat taaatggaga agaaattcaa
    38761 gttatggatc agcttattca tttgttatca cttattcaat ttaagggagg aaatctaatg
    38821 aaagtagttt acaaatcaat caagccttac ggattcgagc aaatcatttt gaacaatcaa
    38881 gaaaatatcc ctgaaaactg tacagagatt aaaccaccag ttcctaactg gagaccaaga
    38941 tttgattttg ataaaaaaca gtgggttgag ttagctactg aagaagagaa aaaagggaat
    39001 gctgttgata gcgtagatga attggctaat attaaggcat tatacgaaac gctgaaggca
    39061 gaaaatgatg aattgaaaca gctaaattct aaagcaatgc taaacaatgt agcaatcaag
    39121 caagaaaatg tcttattgaa agaaaagtca gaaagtttag cgcagttgaa ttcaaaaacg
    39181 atgcttgctt ctgtacaaaa taccaaggaa atcgaagaaa tcaaaaaaca attacaaggt
    39241 gggaagtaac atgtattcat atgaagatat caaactaatg tatgactggg gctgtttcac
    39301 taacgaacaa gtaatggttt tcgttccatt atgcattaca gacgaagaag cagataaaat
    39361 cattaataaa gataagagcg catcttaatt gatgcgtttt tatttaaggt aaaggagttg
    39421 tcacatgatt aatttagggg aatggggaat gatagcagga tcaataaccg ctatcgtttc
    39481 tttgatttta ttagtaatta gaccgattgc tgcatctttc tcgaaaatta ctgagactct
    39541 ttcaaaagta agccacaatt tagatttgct gactaaagat ttagaatcga gcaaatcaga
    39601 tcgattgatg attcatgaag aactaaagaa acacgatgaa agattagata cacatacaga
    39661 aaaattggtg gaacacacgc aacaaatcaa aacttt
//
LOCUS       DACEKH010000016        39031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEKH010000016 DACEKH010000000
VERSION     DACEKH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.016"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..272)
                     /locus_tag="IW662_000754"
     CDS             complement(<1..272)
                     /locus_tag="IW662_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865599.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
     gene            complement(310..447)
                     /locus_tag="IW662_000755"
     CDS             complement(310..447)
                     /locus_tag="IW662_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0865600.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            complement(449..895)
                     /locus_tag="IW662_000756"
     CDS             complement(449..895)
                     /locus_tag="IW662_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865601.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRTDGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLNNVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(896..2962)
                     /locus_tag="IW662_000757"
     CDS             complement(896..2962)
                     /locus_tag="IW662_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ0865602.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNEVLAKVEAVSKNVSSAQNDLTILEDRIDQTNQQIGDLGKLKKMYSNSIDFGG
                     YDYSGNPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGK
                     TYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTAN
                     VNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWLGKAP
                     LGENIANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSG
                     DYRYGNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGD
                     TRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKGLNDAVNVYDPQRVEGLKNFAD
                     GIQIAGDKVISENDCTVYTLTKDNSQSFIDGYATFIKHGKEVIVNGTVKFKKAYAFGV
                     PLDDEVPDEFIARIVHGMLVGSSGGNSVAKAMYVRKDLGTIVTNSDFVVNEWFTFNGH
                     YWVGGK"
     gene            complement(2962..3744)
                     /locus_tag="IW662_000758"
     CDS             complement(2962..3744)
                     /locus_tag="IW662_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865603.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPGGIEDDSF
                     VEMMVFADHDLRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTASVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNESAGMTLIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNDVKITKIEGVK"
     gene            complement(3761..5518)
                     /locus_tag="IW662_000759"
     CDS             complement(3761..5518)
                     /locus_tag="IW662_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ0865604.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYTDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            complement(5493..8324)
                     /locus_tag="IW662_000760"
     CDS             complement(5493..8324)
                     /locus_tag="IW662_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0865605.1"
                     /translation="MSETIYFFDHLQKLIKRKNKRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPSETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPAVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPEATQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTELLKNTYSTLIDSARPLVQFKAEITGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPGVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            complement(8321..9088)
                     /locus_tag="IW662_000761"
     CDS             complement(8321..9088)
                     /locus_tag="IW662_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0865606.1"
                     /translation="MYSFKDTTINLNSSKRFLPTSAMMYDGMYLEDLIEGYQTLTVEG
                     REMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELLNYLYRNE
                     DVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVSDGYIGNP
                     IQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQVTVNGEDC
                     TSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            complement(9121..12240)
                     /locus_tag="IW662_000762"
     CDS             complement(9121..12240)
                     /locus_tag="IW662_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0865607.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEEQMSRVKAISGATGKSFDELRQQAVD
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTIQEAPGKVADWIKNKWTETKEFFSNLWSSIANSASEMWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNVIQGWKNLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLHKGLMGGFNDVQNTVGSMADFIAELFN
                     ANPDVDIAANLKNANKNIGAQVEHKVNMGGSTKPAVFKFNLGRQSFRLFLDDIAQAMG
                     EGADINLEF"
     gene            complement(12257..12544)
                     /locus_tag="IW662_000763"
     CDS             complement(12257..12544)
                     /locus_tag="IW662_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865608.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            complement(12598..12966)
                     /locus_tag="IW662_000764"
     CDS             complement(12598..12966)
                     /locus_tag="IW662_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865609.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFIDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEEETITLDEMDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQAE"
     gene            complement(13012..13524)
                     /locus_tag="IW662_000765"
     CDS             complement(13012..13524)
                     /locus_tag="IW662_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HAQ0865610.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELNLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            complement(13535..13927)
                     /locus_tag="IW662_000766"
     CDS             complement(13535..13927)
                     /locus_tag="IW662_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HAQ0865611.1"
                     /translation="MIKTRDQSIFDELFKISQEKLGYKTYDYKPLDNVGYPFVEFENT
                     QTIHEANKTDIKGTVIVVLSVWGLQKKRKQVSNMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            complement(13924..14295)
                     /locus_tag="IW662_000767"
     CDS             complement(13924..14295)
                     /locus_tag="IW662_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ0865612.1"
                     /translation="MKVSLGYKGVDELLKHLEEAVTLRDVQMVVKTNGAELTKRMQEK
                     ARFNGHWEGDVFVHPTGFTRRSIRMWLLEGGFVAQVGPQSDYSPYLEYGTRFMSAQPF
                     VGPAFNVQKAIFMKDMQRLFK"
     gene            complement(14292..14618)
                     /locus_tag="IW662_000768"
     CDS             complement(14292..14618)
                     /locus_tag="IW662_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865613.1"
                     /translation="MRYDTEVTFVIEKDGYYDPELGEHVEPTLDEKIKLANVTDLGTD
                     RSKVLFGDIKQGAKVIRLLRPYTKEWDYVLIFNKLKSKTEKFEIITERNLRLKNTFIV
                     QEVIAK"
     gene            complement(14615..14953)
                     /locus_tag="IW662_000769"
     CDS             complement(14615..14953)
                     /locus_tag="IW662_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HAQ0865614.1"
                     /translation="MDETLEEVKRSLEVDNEELDKQLTDFIKRISSQLCVRLGFLESV
                     PAALNYIVVECTIKRFNRKGNEGMSSYGQEGESISYGKLLDDFEEDIFAYLKKQNGDT
                     PPYRSVATFI"
     gene            complement(14993..15199)
                     /locus_tag="IW662_000770"
     CDS             complement(14993..15199)
                     /locus_tag="IW662_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865615.1"
                     /translation="MTKYKVAKVFIDVHTKETYKVGQEIDLNKERFDEIEKNLEAFGG
                     GFLEPIKIEEKQVKKPTKSTKKKG"
     gene            complement(15210..16217)
                     /locus_tag="IW662_000771"
     CDS             complement(15210..16217)
                     /locus_tag="IW662_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HAQ0865616.1"
                     /translation="MANIAELFSQRNVLDYVNNRQAPPLLGETLFPARKVQGLEFDIL
                     KAGTRIPTIASVHAFDTEAEIASRVASRSAQELAFIKRKIQLKEKDLIALRNPRTAEE
                     QAFLENEVYNDVFSMVSSVNARVEKMRMEVLANGTVTLDENGLDLVVDYGVPSEHKAT
                     VDFAASSTDVIGLLTTWAGTLDTTPTRILTSTKVRNAILQNAGIKAYFKDAGLLPTAG
                     TLNQLLQQFGLPIIATYDAKYYKENAQGVLVKERYFPENKLVMFGDENPGESIFGVTP
                     EESRLLSGGTNDYQVGNIFAMVYESNLDPVGTWTKASGTALPSFPEADNVFQATVLAE
                     G"
     gene            complement(16242..16595)
                     /locus_tag="IW662_000772"
     CDS             complement(16242..16595)
                     /locus_tag="IW662_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865617.1"
                     /translation="MVYVKKSETFKEINFLKSQQFISFTKQVDHTHAGVVNGVLPAGS
                     VYPANDGKAEGITINDVDVSNGAQPVGVIIDGHILIERLPVKPSDAAQKAMREIKLYD
                     ASGKMIALPAAASGL"
     gene            complement(16607..17248)
                     /locus_tag="IW662_000773"
     CDS             complement(16607..17248)
                     /locus_tag="IW662_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ0865618.1"
                     /translation="MKKRLFMPMSLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENTTGKTFSRDEVAKMIAAETKKAVTAAEEKWRAEKDEAAKLAEMDDKEKADYEKQQL
                     EAKLAEFERKEVLSKMSEQASEMLSEKGATPTKEMLRLIVSEDAETTSNNVKTYLASV
                     EAEREAIKAEYEKRLGGKVPLDGNGATISRGAQLAKNANNQTKKPENDPWAIK"
     gene            complement(17363..17545)
                     /locus_tag="IW662_000774"
     CDS             complement(17363..17545)
                     /locus_tag="IW662_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865619.1"
                     /translation="MPDMENEEAIVRAIASLNQILQNEKVLRVSVEIDSEWEDGIKSF
                     DISVKYLPSGDINGAK"
     gene            complement(17584..17805)
                     /locus_tag="IW662_000775"
     CDS             complement(17584..17805)
                     /locus_tag="IW662_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865620.1"
                     /translation="MHLYQNDAQFKEDYDAVKQHLIDVLEPIVKEVYEAARKVIEPVV
                     QSITELVQSNPEYFKALQNNMNQSMLQSR"
     gene            complement(17883..18836)
                     /locus_tag="IW662_000776"
     CDS             complement(17883..18836)
                     /locus_tag="IW662_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ0865621.1"
                     /translation="MKSQDYFIKREKAWQEQQIKDDKKRMNEIKKRLQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRADEMDVKAFARKAKKYVKEKDFSPTANKELKLYNLTM
                     RVNRLELLKANIGLELIATFNDMDKYFSGELTSAGLKELQRQAGILEMTIAKSGYAKL
                     VEQVINSSFRADGFATFSERLWMYQAELKADLDKLLVRSVTMGRNPKQLAPELSKFLT
                     EEGRENTKFNTQRLMVTETTRVQTGIQEQSYRDADIDQFVFISEPTACRYCLPLNGKV
                     FDLKDMSPGVNCAPMHPFCRCSTAPYVDREAFEKSLKERGL"
     gene            complement(18841..19104)
                     /locus_tag="IW662_000777"
     CDS             complement(18841..19104)
                     /locus_tag="IW662_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865622.1"
                     /translation="MKARKKPVTVEIVQLKMLSARSYRKCKEFVGEAWVDHNNMPNGL
                     PGIETLEGTMEISDGDYIIKGVHGEFYPCKPDIFLETYEIIEE"
     gene            complement(19117..19347)
                     /locus_tag="IW662_000778"
     CDS             complement(19117..19347)
                     /locus_tag="IW662_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865623.1"
                     /translation="MNLKEQMMNQYQKKDSEKIKDAIAEAMKIGRNEVLYGKDVITDD
                     IRKEFQDGGFTVEDYEDKHTIDAKIELVRFSW"
     gene            complement(19301..20935)
                     /locus_tag="IW662_000779"
     CDS             complement(19301..20935)
                     /locus_tag="IW662_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0865624.1"
                     /translation="MEQNIQLLGQQRFDEEANLVYKVPVSQLPKIDMLDQQTGEVSEF
                     INFKHEDMWKMIVGFIKHHRERQVPRLKELKRYLNADNNIKRRPNKPDGRADNRIASD
                     FANFIVSFKQGVLLGNPISYNGDKVIVERINRFASESNEDYHNQLMSRDAFGLGRAYE
                     WVGRDEYGKETIAKFDAEQTFVIYDNTKDRNSICGVHYFVEKFLDKSFTRIELYTNCG
                     YNYYFTAKDDDLENAVLDEDGEVQSYFDTVQINEWINNEERTSDFEHVMDSIDAYDLS
                     RSEMANFQQDSSEAYLVIKGNPDTADDQEGDNSKLAVFQAMMQARMLVLGDKKIYDNN
                     VAGAEPDAYYLKKEYDVAGMEANDSRTVADILRFTSLIDFTDENIGSNQSGIGFRFKG
                     WGSDNDRKNKERMVKKAIMRRLRLLTHSWSIKDGLDKPRGLIDTVKAFFVSDEKQQEQ
                     LYNKVNEIQIQFTPNVPQSDEEIMSVIAGMVGIVSDQTLCEMAERLTGVPFEEELKRL
                     KKQAVSGVFDSDKETDTEVEEDESQRTDDEPVSEEG"
     gene            complement(20946..22244)
                     /locus_tag="IW662_000780"
     CDS             complement(20946..22244)
                     /locus_tag="IW662_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAQ0865625.1"
                     /translation="MAKKLSEFLPPKFHSVWRATLNQDILNIVCKGGRGSGKSSDIAH
                     IVTQLLMRYAVNAVGIRYVDNTLEQSLYEQMKWAIEQQGVSHLFKFNKSPLKITYLPR
                     GNYMIFRGAQNPERIKSLKDSKFPFAIGWIEELAEFKTEDEVTTITNSLLRGELDDGL
                     FYKFFYSYNPPKRKQSWVNKKYETSFQPKNTFIHHSTYKDNPFISKEFLSEVEAARAR
                     NPRRAEWEYDGKAIGSGVVPFDNLRIEAGSITDEMVANFDNIRQGLDYGYATDPLAFV
                     RWHYDKKRNCIYAIDELYEVKCSNRRAAQWIKSNKYHYQDIIAEVEPKSNAEMRNEHD
                     ISKIRQVTKGPDSVEYGEKWLDDLDAIYIDPIRTPNIAKEFENIDYQTDRDGNPKPRL
                     EDKDNHTIDATRYAFNDDMRKQPEPVNVKKTIDTFKKLGL"
     gene            complement(22237..22692)
                     /locus_tag="IW662_000781"
     CDS             complement(22237..22692)
                     /locus_tag="IW662_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAQ0865626.1"
                     /translation="MSKLNPKQQAFADEYIITGNAYQSALKAGYKENYAKNAQEKLVE
                     KGGKVSDYIQEKLKEVQTKRHLTMEEALAITASIAKGEPQRFEVVKRDPYTNEIIERE
                     VSEYSAGFKERNQALEHYYKINAAFVDKQKVEISEIPTFIDDISSDDDG"
     gene            complement(22718..22942)
                     /locus_tag="IW662_000782"
     CDS             complement(22718..22942)
                     /locus_tag="IW662_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865627.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIVEMTREAT
                     PNEIDKCKLIYCGHGYFSEQNIQTNIKKYH"
     gene            23441..23620
                     /locus_tag="IW662_000783"
     CDS             23441..23620
                     /locus_tag="IW662_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAQ0865628.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITEDSQVIDMTK"
     gene            complement(23613..24026)
                     /locus_tag="IW662_000784"
     CDS             complement(23613..24026)
                     /locus_tag="IW662_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865629.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQIKYQGMDRKRLIDII
                     EQQRLELIQKEAVCKEYKKHLEQVIEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTP
                     VFNSVKSEKLESISTALVEKAAEMNSLANEVSRLI"
     gene            24317..24592
                     /locus_tag="IW662_000785"
     CDS             24317..24592
                     /locus_tag="IW662_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865630.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            complement(25050..25463)
                     /locus_tag="IW662_000786"
     CDS             complement(25050..25463)
                     /locus_tag="IW662_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0865631.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(25540..25836)
                     /locus_tag="IW662_000787"
     CDS             complement(25540..25836)
                     /locus_tag="IW662_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865632.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            complement(25833..26390)
                     /locus_tag="IW662_000788"
     CDS             complement(25833..26390)
                     /locus_tag="IW662_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0865633.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            complement(26387..26806)
                     /locus_tag="IW662_000789"
     CDS             complement(26387..26806)
                     /locus_tag="IW662_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ0865634.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            complement(26803..27048)
                     /locus_tag="IW662_000790"
     CDS             complement(26803..27048)
                     /locus_tag="IW662_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865635.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            complement(27008..27364)
                     /locus_tag="IW662_000791"
     CDS             complement(27008..27364)
                     /locus_tag="IW662_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ0865636.1"
                     /translation="MDLITQYSDIILKKIMMKIQNDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            complement(27364..27669)
                     /locus_tag="IW662_000792"
     CDS             complement(27364..27669)
                     /locus_tag="IW662_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865637.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(27666..27827)
                     /locus_tag="IW662_000793"
     CDS             complement(27666..27827)
                     /locus_tag="IW662_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0865638.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            complement(27824..28126)
                     /locus_tag="IW662_000794"
     CDS             complement(27824..28126)
                     /locus_tag="IW662_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865639.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(28288..28557)
                     /locus_tag="IW662_000795"
     CDS             complement(28288..28557)
                     /locus_tag="IW662_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865640.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(28569..29318)
                     /locus_tag="IW662_000796"
     CDS             complement(28569..29318)
                     /locus_tag="IW662_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0865641.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(29321..30007)
                     /locus_tag="IW662_000797"
     CDS             complement(29321..30007)
                     /locus_tag="IW662_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865642.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(30013..30684)
                     /locus_tag="IW662_000798"
     CDS             complement(30013..30684)
                     /locus_tag="IW662_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0865643.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(30677..31018)
                     /locus_tag="IW662_000799"
     CDS             complement(30677..31018)
                     /locus_tag="IW662_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865644.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(31196..31894)
                     /locus_tag="IW662_000800"
     CDS             complement(31196..31894)
                     /locus_tag="IW662_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ0865645.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            31981..32451
                     /locus_tag="IW662_000801"
     CDS             31981..32451
                     /locus_tag="IW662_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865646.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(32494..32673)
                     /locus_tag="IW662_000802"
     CDS             complement(32494..32673)
                     /locus_tag="IW662_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865647.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(32660..32998)
                     /locus_tag="IW662_000803"
     CDS             complement(32660..32998)
                     /locus_tag="IW662_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ0865648.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(33014..33217)
                     /locus_tag="IW662_000804"
     CDS             complement(33014..33217)
                     /locus_tag="IW662_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0865649.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(33232..34008)
                     /locus_tag="IW662_000805"
     CDS             complement(33232..34008)
                     /locus_tag="IW662_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0865650.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            34307..34627
                     /locus_tag="IW662_000806"
     CDS             34307..34627
                     /locus_tag="IW662_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0865651.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            34645..35076
                     /locus_tag="IW662_000807"
     CDS             34645..35076
                     /locus_tag="IW662_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0865652.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            35206..35667
                     /locus_tag="IW662_000808"
     CDS             35206..35667
                     /locus_tag="IW662_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ0865653.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            35701..36336
                     /locus_tag="IW662_000809"
     CDS             35701..36336
                     /locus_tag="IW662_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ0865654.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            36449..37084
                     /locus_tag="IW662_000810"
     CDS             36449..37084
                     /locus_tag="IW662_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865655.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            37383..38522
                     /locus_tag="IW662_000811"
     CDS             37383..38522
                     /locus_tag="IW662_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0865656.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(38591..38664)
                     /locus_tag="IW662_000812"
     tRNA            complement(38591..38664)
                     /locus_tag="IW662_000812"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38628..38630),aa:Arg,seq:cct)
BASE COUNT        11089 a         8179 c         6354 g        13409 t
ORIGIN      
        1 aaagttttaa tttgttgcgt gtgttctacc aatttttctg catgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtgacttact tttgaaagag tctcagtaat cttcgagaaa
      181 gatgcagtaa ttggttttat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcgctc cccattcccc taaattaatc atgtgacaac tccttgaatc aaaataaaaa
      301 gcacatcaat taagatgcgc tctcttcttt gctaatgatt ttatctgctt cttcgtctgt
      361 aatgcatagt ggaacgaata gtcgaacttg atcgtcagta aaacagcccc aatcatacat
      421 cattttcaca tcgctaaaac taaacatact actcacctcc ctttgaagct ggatttagtt
      481 gctctttaat ttctgaaatg tctttgctat tttgtaacga agcaagcatc atttttgaat
      541 tgatttgtgc taaactatcc gctttttctt tcaatgcagt attttcctgt ttaattgcta
      601 cattgtttag catgagtttg gcgttgagct gttttaaatc gccgttctca ttttctaacg
      661 actcatacat cgctttgaga ttgtttaaat cgttgtgatc tagtacattc actaaaacaa
      721 tccattggtt cagtttagga tcaaacatct gatcagcaat cgttagcggt tcgccatcag
      781 tacgaattcc ttcgagtggt ggaacatcgg taaatggaac agtcataacc atatcatcaa
      841 gcacttgtcc agcatactct ccacctgttc gtccatattt ccaaatgttt ttcatttatt
      901 tccctcctac ccagtaatga ccgttgaatg taaaccattc gtttacaaca aaatcgctat
      961 ttgtcacaat tgttcctaaa tcttttcgta catacattgc ttttgcaacg ctatttccgc
     1021 cagaagatcc cactaacatg ccatgaacaa ttcttgcaat aaattcatct gggacttcat
     1081 catcaagtgg tacaccaaaa gcataagctt ttttgaactt tactgtacca tttacaataa
     1141 cttcttttcc gtgtttaata aatgttgcat acccatcgat aaacgattga ctgttgtctt
     1201 tagttaatgt ataaacagtg caatcatttt cactgatcac tttatctcct gctatttgaa
     1261 taccatcggc aaagtttttc aaaccttcaa ctctctgagg atcatacaca ttaacagcat
     1321 cattcaagcc tttttcagta tattcaggtg tgacatccca actgtaatca ttgggattgt
     1381 tactgtcttt caagccttca ccaaagtatt taaactgact aatattcggg gttcgtgtgt
     1441 cgcctttttc gatcttgagc cagtcaattt gacatgcacc tgttgttgat tggggatact
     1501 ggataatagt taaccgtttg gggtagttaa ctccaacctt ctgcggtgta aaagtcagac
     1561 cccatgtatc aactaaccct tctactggct ctaagttacc atatctataa tctccactat
     1621 cctcgttgta aacaatgaac gtttggatag gtggtttggt tgccttcatg gtaattgtat
     1681 aggtttgacc taaaacaaat tcttcttcca tgttagcttg gtataggctg taggcgctag
     1741 atttgattgg aaacgtaaca gacttattag caatattctc acccaaaggc gctttaccta
     1801 gccagtaagg gtcatcaagt aagtttggct gatatggggt ggctgttgag ccttcttcga
     1861 ttttgatgtc gtacaactta aatccgccat ttattttatc cctgtcaaca aagctaatac
     1921 tcatataaaa tcgatctaaa tttgtgattt gataattaac gttagctgta cctttgattg
     1981 ttatttcttt ccctacatca tctgttgtaa tatttgtact atttgcttcc aataatatct
     2041 tttctcctgg tgatgtacga tacaccaaac gtaatttatc aatagctcct gtagttcctt
     2101 catcaaatcg aactttcgca ctcagagtat aggttttccc actagcaagc tgaggtgtat
     2161 taatacgcgt aaacataata atgcttcctg taccatcaga agtaaaatgt agcttttcgt
     2221 tatccgaagt tagtgacccg tggtaaccaa cgttaaaatc gctcgatttt agtttggaca
     2281 ttaaattcgg attcccacta taatcatagc ccccgaagtc gatgctgtta ctgtacatct
     2341 ttttcagctt gccgagatcg ccgatttgct ggttcgtttg atcaatacgg tcttcaagta
     2401 tagttagatc attttgcgct gaactaacat ttttagaaac agcctcaacc tttgctagta
     2461 cctcatttac tgcatctgtt gcttcttgtt tgactccatc aagtaattgt tgaaagtctg
     2521 caataaagta gtctgcttta tcttgcgcta gtccgtcgat tgctgaacgc ttcatacgga
     2581 aggtgaagcc taagttatca cttgttgatc cgtctggata ctcgatgtaa acataagctt
     2641 ccaccatacc gttataagct ttcatcgctt ctggcaagat atatacgacc tgtccattca
     2701 agaagctttc agtgatgagg tttttcgtga taaaaggaat tggctctttc gtgatcattc
     2761 catctacttc atcatagata aacatcaaca agcgcaagtt agctcctagc aagtccgccg
     2821 gtgtgccgtt ctgttgttcg acgttgaact ctaacgctat ttgattatcg tatgatttaa
     2881 atacaagccc cgttgcttgc aaatcatagt ctttcggctg tgtaggtact ttgatagagc
     2941 cttttttgat gacatgcgcc attatttcac accttcaatc ttagttattt tcacgtcgtt
     3001 gctcattgcg ggattggaaa cattgccgga tgaaatatct aaagctctgc cgtttgacat
     3061 cgttatcttt cgcgcttcga ttgtcagttc aaactcgatc aacgtcattc ctgcactctc
     3121 gttccataaa ttagcttttg taatccgcgc gtatctttga cgtttcactt cttctacaaa
     3181 gtctccgtca ccgtctgtgt agtggatacg aagcgtttgg taacggaaag tatcatctgg
     3241 cagattgact gattgtcctt ttcttaattc tccttcaaaa agaacagaag cagtgtaaac
     3301 gcgccgccaa cctaacgagt tataaccctc taccggttcg ctgttatgtg tcattttgat
     3361 gtaaatttcg ccgctataac gagcaaaagc gacgattaat tttctcaaat cgtgatccgc
     3421 gaataccatc atttcaacaa aactatcatc ttcaatcccg ccggggttgt ctgcacccca
     3481 tccggctttg gttgcgtatt ttccgggtgg aatatccaag atgttcggat atttttgtgg
     3541 caacttataa tcgcgtaacc aacgcccacg cgcttgcata actgtttctg gtgtagcaaa
     3601 gcccgcgttg ctttcagtag ctaggacgtg cgcgtcgtga tccgagccgt cgtaatggat
     3661 agccgcttga cgcaataacc atttcacagc ctcttcataa ctcatttctt caaaaatatt
     3721 tggtttgctt tgaaaatcta acatttaatt gttcaccgcc ttaatagtta taagtgaagt
     3781 ctctcgcgcg ccctacgcct ttgcttgtca cttttcttgt tgctgtgtca acttctattt
     3841 tataaaacgc taattcatcc ggtgtatcaa cttggctagc tgtatgagag aagccgacgt
     3901 cgcaaagaat catttttact gtcccaaacg ccccttcgac ttgttcgtgc caatgcccgc
     3961 agaaataacc tactacaacg ccggcgcctt ttgtattcat tgcgaaggtc tttaatccgc
     4021 cgaacgaacc gtcattcggt tgtcctaatt tagaccaatc gatagttacc ggactaccag
     4081 atttaaaccc ttctatcaac gtgctaatca tgttttcatt gcgaacggga aacttgctga
     4141 gacctaaagg cgtatgtcct actaacgcca catgatagtt tctcggaacg ttcactaacc
     4201 attctccgaa agcgttaatt tgtttggcac tgaaagctcc cggcgctgta tcgctgtaac
     4261 cgtccgtgta cttgtcacct gtgccgcctt cataaaaatc acaagtatcg aaacggtaaa
     4321 ttgctgcgtt tttatctggg aacaacacgc caccgtacaa accgttccaa tactcttcga
     4381 agtcagcgtt gcataacatt ccctttcgct tgcgccacgc ggggtcgaaa caagcgtcgt
     4441 gattcccttt gcagataata accggttttt cttgtcctgc taccgctgcg ttagtgaagc
     4501 gtttgagcgt gcctaacatg gaatgtcgcg cgctccattc atcaataatg cctatatcgc
     4561 tgccgagtga accaagtccg ccgtcgatat tgtctccgcc gtaaatcatc acatctgttt
     4621 tgttccctaa tttttgaaat tgagggattg cgcgccaatg tcttaaataa gaagcatctt
     4681 tgtaaccgac gccatctata cgcaagttgt gattatctgc gtgaatgtct gtaatgaaag
     4741 aaaagttaaa tttgctgtta ttaacactgt tgacaaccgt attcaaattg cgcggcacta
     4801 ggtcaacgta tttcatcgtg tcgtaatcaa aaaagcgtgt tgtttctcta atttgtgagc
     4861 tgccgactgg cacttggtat ttttggttta aacgatcagc taatgattcg tagtcgcctt
     4921 tagcttcatt cagaatgtta atgatcatac cacctggatc gatattttcc agtatttcac
     4981 gattatcttc taaccactgc tcccagtcat ttttgccctg atccatgtaa tctttgaatt
     5041 ttcttagcaa atcctcaaat gtccacacat agccagagtc acgtaactgg cttctagata
     5101 ttccagaaat gactcgatag gtaaaatcct gtgtgctaaa ttgttcactc caagttccat
     5161 caccattaag tgatcggaaa ctgaaatgtg cggtgttttc accaccccat tgccagtcag
     5221 gctcacttaa ggtgtaaaca agccttgctt gcgctggact gtattcttgt actttttgtt
     5281 caacaggttg gttttcgcca aattttgttg tattaataaa aaacggcact aggccctcga
     5341 atgtttttag tttgccatgt tccaccactt caacaacgaa cttttgcgtt aaaacatccc
     5401 cttgccgaat tcgaaccaaa tttattccgt tgtttggttc ggtggtggat aggaccattt
     5461 tatgctgcgt ttctgccatg actatccctc ctttagaaat cgatgtagtc tcttgcattg
     5521 tggaaatgac ctgaagaaga tggataaaac tcatccataa attgaaaatg aagatgttct
     5581 ccagtggatg gtcctgttgt ccccatcagt ccaatttgct gaccagcagt taccttctga
     5641 ccttttgaga cgtctactcg gctttgatgc gcgtatcctg tatacattcc atcagcgtgt
     5701 ttaatcactg tccaatttcc ataccagtca aagtaatttg catcacctgc aacaatcact
     5761 tcgccatccg ctgaagcaaa aataggtgta ttaggatttc catttacaag gtcaataccg
     5821 ttatgaaatt cttgtgctcc tgtgattgga gaagtgcgcc agccaaattc gctcgtcact
     5881 gtgattggat ccgcaatcgg cttcatatat ccttttgata caggaatttc caaatctttg
     5941 aacttgtcat accattcttg tgaccatgcc gtccgttctg gatgtggatc acgtggacgt
     6001 tcaaagttag ccacgaatgc ttgtgctgct gtgttgatat cggtcagatt catgaattgt
     6061 gtccatgtgt aaggataagc gctagttgcg atccattgac catttggtgc atgccacatc
     6121 aacaatttga actgtgccgt gattgtgtct ggatcatcac tgatgccagc ctttgtcatt
     6181 aaattgatca tgtagacacg tccgctagtt gcgccagaag aatcggtcca ttgccataca
     6241 ccataaccga accctggtgc gccattgccc tcatcagcgg ttggattagc atctgattct
     6301 ccttgtgcat ttccaagtaa ggctgcagca gcttgttgtg taaaaccggc acctattgcc
     6361 gttgcccaaa tttgccaata tcgtttatcc cgatcagtag ttactcctgg tgggtattgt
     6421 ccgttccaac cgttatcgtt ccctccggaa ttgcctcctc cattagtgtc aatttttgta
     6481 ccgttaacat agagttcttt tacatctagc cgtccatcta tggttatatt tccttctgaa
     6541 aaattgccgt caccataaag attatatttg cgcttatcag cagtaacatc tgctggaatt
     6601 tgaaaaatag gattccctcg atcgccaccg tccccagcat taatggaaaa aatatagttt
     6661 ggctctttcc agacagcaaa gccgttaatc cctttagaac cataagtaga tacaattgag
     6721 cccaagcgtt ctccatgtac atcatctagt ccagtagact tgacttgttt ttcaaaagaa
     6781 agttctcccc cctctgccac taattggaaa tctttatcgt caaatgtttt taaagctacc
     6841 ccttgcacga ggataccaga cagaatgcct gcgcgaataa aattagcatt gaaagttcca
     6901 tccaacgtcc aagcggtcgt gctatcacca ttgtgtacat cttgaattgt ttgccactca
     6961 ccttttttac attgtttgaa agatatccct gagttatttt ggaccataaa aaagcgtgat
     7021 ctaggaatgt taggtccatc catataaaca gtttcataga tttctctact atcactaaca
     7081 ccagcttcaa ttccatttac ccaataaata gaaccgccat tatctcctgc acctcgcata
     7141 atgtcatctt gatattttcc aatctctgtc gattcgtaaa atgtcatttt gctagattct
     7201 aaactattaa tattattgac aatagaagcc gtttgttttc taacatcttg tgttaaatta
     7261 tcccctagtt cgatattcgt ttgaccggta agccgattga atgtagtttt ataaatacga
     7321 gttttatagt gataaccttt atcgtatctg tgaatagtca ctgtatttcc tatcacatct
     7381 cctccagtga tttcggcttt gaattgtact aacggtctag cagaatcgat taaggttgaa
     7441 taagtatttt taagtaattc tgttggatca tctatatcat caaacactac cacggtttct
     7501 cgctttctca ttgatccatc tttttgtggt ataccatact tttgagttgc ttccggatct
     7561 tcaagccaat tttggccttt aggcttatct aaaggatcac cattcgactt tttccattca
     7621 acatcagtga attcaattct tctaccgtat ccgtcaccaa cctcttcgcc tcgcccgcga
     7681 cctatcattg aagttgagat ggagcttcga tctatctctc ttacaactgt taatgcttta
     7741 ctaccatata caaaacgtgt attcgattct tcaccaattt gttcatatac ttcgatccat
     7801 ttatccttta ttccatcaga attcaaagaa cacctaaaga caaattccat acctaaggtt
     7861 tgcaattctt tcaacgcttc ttttacagag acatagtaaa aagttgcagt tactgctggt
     7921 aacattgctt ctacgtgacc aacgcgccaa tttccttcag taaattcaat caatcgatca
     7981 agaacatttt ttaagggctg cccactcggc ctaatatctt tgatgatgta agcatctaat
     8041 tcatttgtcg caaatcctaa ccctgtaaac tctaatgttt cagatgggtc gctcacttta
     8101 gtaattcgat acaacgaaaa agacgactcg ttttcacgaa tcgccatata tcttgcatct
     8161 tctatttctt tatcatattt tgtcgtaacg taaagagtat ctttcattag atcactctta
     8221 tcagaactaa tttctttttc ttgggagact tcaattaaac ttcttttatt ttttctttta
     8281 ataagttttt gcaagtgatc aaagaaataa attgtctcac tcaaattgtc gcccccctat
     8341 agaatatttt aagcttccca ttattgctag ttatcttctg accttgctta agataaaagt
     8401 tctcaaaatc actttctaaa tcaatcatag aagtacaatc ttctccgttt acagttacct
     8461 gctcatcgga aaaatcaaaa accaacacgt ctcctgtttt tattgccgca tcagttatcg
     8521 tgatattttg ttctccgttt gtaattttga ttgaattatt catggataaa gtaacttcaa
     8581 tttttcttgg tgttatagga aactgtattg gatttccaat atagccatca ctaacacatt
     8641 ctttcgtata ctttagtgga tccgcacaga atacattaaa actcgaaata atcgagttgg
     8701 agtctcctgg aacagtatca gttgatgtat agcgaccgta gtaataataa tctaattcat
     8761 catgaaacct aatttccacg tcttcattcc ggtataaata attcaacagt tctttgaatt
     8821 taaactgtaa tttttctgga tctctgtctt ctaacttgta tgttattttt agtgttcttg
     8881 aaggtatttt ctgatttgta atgattgaac caatttgtat ctcttgctgt tcaacttcta
     8941 cagaaagcat ttctctacct tccaccgtaa gcgtttgata accctcaatc aaatcttcta
     9001 aatacattcc atcgtacatc atggcagacg ttggaaggaa tcgtttagaa ctattgagat
     9061 taatagttgt atctttgaat gagtacattt tattttctcg ctgatccaaa atattccctc
     9121 ctaaaattct agattaatgt ctgcaccttc gcccatagct tgtgcaatat cgtccaaaaa
     9181 taatctaaac gattgtcttc caagattgaa tttaaataca gctggtttag tagagccgcc
     9241 catatttact ttatgttcaa cttgtgcacc aatgttttta tttgcatttt tcagatttgc
     9301 agctatatct acatcaggat ttgcattgaa aagttccgcg ataaagtctg ccatacttcc
     9361 aacagtattc tgtacatcat tgaatcctcc catcagacct ttatgcagac cattcataat
     9421 agcctgacca gctggaatca atagctttct atcgtattgg ataggtcctt tgtgttcacg
     9481 aatccagtca ccaatacctc caacaaaatc ttgcacagat ttccatgcat tttgtaaccc
     9541 ttctagaaaa ctatccatga tagcttttcc ggctgctagt aaatcgatat ttttcaagtt
     9601 atcaaaccag ccagttactc tatcaaccgt atcactaaca gcatttacta aattatccca
     9661 tgcctcttga gcaccattta ctaaattgtt gaaagtatct atagtgcctt gttttaggtt
     9721 tttccaacct tgaataacgt tatctttggt tccagtaatt aaatcaccaa tccaagattt
     9781 gaatgaagtc caaatatctt tagcaccttg aaccgtatta ttaaataaat ctatcgttcc
     9841 ttgctttaag ctgttccaac tttttataag agtttgcacg atattattaa ccgtttggaa
     9901 aaaccattgt tttagattat tccaaagttc aattgcacca tattttgtat caatccaagt
     9961 ttgaattatg gagaatttta gatttatcca catttgaatt gcactgtatt taatatcaat
    10021 ccataaatta gtaaaaaata gtttcaaatc aatccaaatc atagtcgctt gataaactac
    10081 ttcattccaa atattagaaa tactttgtat gaatccagtc cataaactaa cggcactatt
    10141 tactattgca ccgatatatt ccgtaaatat attttttaca gatgtccaaa tatttaccac
    10201 tgattggaca agtgtgttcc agattagatc taaatcctct ttcatttgtt cgaagtcacc
    10261 agtaattaaa tctataataa acagtagcgg cgcagctatt aaagacttaa tgatttccca
    10321 agcatttact attatgtttt taacctcagc aaatatactt gttattgctt tcactatatt
    10381 agagaacaca tctgaaaagc tacttacaaa tggaccaata tattttagta taaaatcaaa
    10441 accagatttg attttacttg taattgtttt ccatgcagta gatatagtat ttttaaaccc
    10501 ttcccatttt tcaccagcac ttgaaactaa atcatcaata actgatatga caccttcttt
    10561 taaactattc cacatctctg aagcagagtt tgcaatactt gaccataaat ttgaaaagaa
    10621 ctcttttgtt tcagtccatt tattcttgat ccaatctgcc actttcccag gagcttcttg
    10681 aattgtagtc caaacattgt ctgcgccttc tttaatggac ttccataaat tgttaaacca
    10741 ttctcctgta gatttccatg cattctgaat ccattctact gccgagctta cagcagactt
    10801 gattccctcc cataagccaa tccaaaagtt tctaaaatct tcactcgtat tccaaagata
    10861 gatgaaacct acaacgagta gtgctaccgc agctataacc agaaaaaccg gatttgtaag
    10921 aagacccatt gctttactta atccactaaa caattttgaa ctttcactta aagctttaaa
    10981 agctactttc accctattga tccatataat tactgaacca atcataaaaa ttaacggtcc
    11041 tatagcagcg acaattgctc ctatggccac tactaatttt tgagttgatt ctggagcact
    11101 tacaaatttt tctactaagc cggatatagc atctgctact tttttgatgg atggtgctag
    11161 aatcttttga attacaatag ctgctgactc aaaagctcca aacatttgct cgatggaaga
    11221 attcatatta tcttgcatgg tccgagccat atcgtcagct gcaccatcag aatctttcag
    11281 agattttgtt aatttgccca atgaatcagg tcctttatca atcaaagcca tcatccctga
    11341 taatgattct tgcccatata gtgttactaa agcattttgt tgttgttcag gcgtcaggcc
    11401 ttcaaaagct tttttaagta attctacttg agtttttaaa ggtttcattt taccgtcagc
    11461 atcataaaac gaaacaccta aattatccat tgtatcttgc atagcctttg ttggccttgc
    11521 taacctagac aatgctcctc gcaacgttgt acctgcttga gaacccttaa tacctgcgtc
    11581 actcataata ccaatagctg ctgcagtttc ttccaaagaa atacccattg aattggctac
    11641 aggagcaaca tacttcaatg cctctcccat gtctccaact tcagcattgg tatccgcagc
    11701 agcacgagca aatacatcag cgacatgtcc tgcttcactt gcttctaaac caaatcctct
    11761 caaagcagta gcagtatttt cagaagctaa agccacatcc cctccagata cagctgctaa
    11821 gtctaaaaga cccggcattg ctttcatgat ttcttgtgcg ctaaatccag cagaagctaa
    11881 gttttccatt ccagcagctg actccttagc gctaaatgct gtcttagcac ccaaatcaac
    11941 agcctgttgt cttaattcat caaaagactt acctgttgcg ccagagatag ctttcactct
    12001 actcatctgt tcttcaaaat cgccaccaac tttagcagct gctacgccta ctcctataag
    12061 aggagtggta atatactttg tcattgcggc accagtacct tgcatcactt taccaacggc
    12121 ggttgtcata ctatttgaat tcttttcaaa agttttaaca gcatcttgtg catctttaaa
    12181 agtctttaca aatccactat ctgtggcttt taataaggct tcaacagaaa attgttccat
    12241 gattttcctc ctttcctcaa gagttagctt tagttagtaa gctttggaat tttttatctt
    12301 gttttgaaag ttcggaaact cccatgattg aatcttcgat tttttgataa ttaaagaatt
    12361 cttcaaagga tcgatataca ggaactgtct ttttgcctac ttttttctcc gcttggactt
    12421 gctgatttgc ccacgctaat tcgtgaatca acttttcttt gtcaagccaa gataactggg
    12481 ctgcagtcat acgaatgttg tattcatata acgtcattct ttcgatatct gagatattgg
    12541 tcattcccaa atatcgaaaa gaattgataa gaatttgttc gtatgccagt gcagaatcta
    12601 ttccgcttgt tgtttttccg cttctttcaa tttctgattc aggtttcgga ccgctaactt
    12661 tcccgcgttt gactccgcca attcttttag gacttcatca aacaattttt cgatgtcttt
    12721 aacttcatcg atgtaatcat ccatttcatc caacgtaata gtttcttctt ctgttctatt
    12781 tgctatttct aagactcgtg acaacgtgtt gacattataa gaacgtaatt ccggtaagac
    12841 ttttgctgag agtcccattc cgaattccat atttccatcg atgaaaggca tcactttgtc
    12901 taattcacgt acaaatttag tgccaaattt aaacgaatat tctttacctt taatttttaa
    12961 tttcaatgtt tttcatcctc ctaaaataaa aaagagagca tctaagccct cttatgctcc
    13021 tgtcgaagtt gctttcacgg tatctttgaa tgcatattga acaacatcgg cttgatcttc
    13081 tgtcaaggtt gcataaccat cttggccaac accatttact gcaaatgata aatttaattc
    13141 aacgttatcc tctgcagcag ccgatggagt aaattcagac acatatgctt ggtaataagt
    13201 agctttgtac ttatttgcat tatcatctgt tccctgttct gctttgttga tttcccaaat
    13261 ttcaatgata tcgccattta ataaggcttg tttcatttca tctacatgag aatctccttt
    13321 agcaactatt gaagtagccg aaaaatcata ttcaaccggg cttaaacttt gaacgtttcc
    13381 gtcttttgtc actgtagagt ctgaatctct tgataagcca ttttcgtgtt ctgtttgaaa
    13441 tgccattttc caagcagctt cctgagtttc ttttttcaat aagcgataaa gcaaaatgac
    13501 atcaatacct tttaatgctt ccatgttctt cctcctatct aattctaaat tcaagtgtga
    13561 caaccgctcg ttttaggggg gtattggttg ttgtgtcgtc catcacttga attccacttg
    13621 cttgataatt taaagcccaa taatagcctt ctgtggcttc tatcaatcta gcttcattaa
    13681 aaagagcaga tgccatattt gacacctgct ttcgtttctt ctgtaatccc cagacggata
    13741 aaactacaat cacagtacct ttaatatcag ttttattcgc ttcgtgaatc gtttgagtgt
    13801 tctcaaattc cacaaaagga taaccaacat tatctaaagg cttgtaatca tatgttttgt
    13861 atcctagttt ttcttgggat attttaaaaa gttcatcaaa aattgattga tctcttgtct
    13921 taatcatttg aataacctct gcatgtcttt cataaagatt gctttctgca cattaaatgc
    13981 gggaccaacg aaaggttgag cgctcatgaa tcgagttcca tattctaagt acggactgta
    14041 atcactctgc ggtccaactt gtgcaacaaa accgccttcc aacaaccaca ttcgaattga
    14101 tcgtctagtg aagcctgtcg ggtgcacaaa cacatcccct tcccaatgtc cattaaagcg
    14161 ggctttttct tgcatgcgct tagtaagctc ggctccattt gttttaacta ccatttgcac
    14221 atctctaagc gtgacagctt cttccaagtg tttaagcagc tcatccacac ctttgtaacc
    14281 caatgaaact ttcactttgc aatcacctcc tggacgataa aagtgttttt aagtcgaagg
    14341 ttacgctcag tgataatctc gaacttctcc gttttgctct ttagtttgtt gaatattaaa
    14401 acgtaatccc actccttggt atagggtcga agtaaacgaa taacttttgc accttgctta
    14461 atatctccga acaaaacttt cgagcgatca gttcccaaat cagttacatt agcaagcttg
    14521 attttttcgt ctaaggtcgg ttctacatgc tcccctaact ctggatcata atagccatct
    14581 ttttcgataa cgaaagttac ttctgtgtca tatctcatat aaaagtcgcc acacttctat
    14641 aaggaggggt atccccgttt tgcttcttta gatatgcaaa aatatcctct tcaaaatcat
    14701 ctaaaagctt tccataggag attgattctc cctcttgccc atacgagctc ataccttcgt
    14761 tacccttgcg gttgaatctc ttgatcgtac attcgactac gatatagttt aacgccgcag
    14821 gaacgctctc taagaagcct aaacgcacac atagttggct tgagattcgt ttgataaagt
    14881 cagttagctg tttatcgagt tcttcgttat caacttcgag cgatcgtttc acttcttcta
    14941 aggtttcgtc catgactgcc tcctttcaaa aataaaaagg ctagtcaaat gactaacctt
    15001 tctttttagt tgatttagtt ggtttcttaa cttgtttttc ctcgatctta atcggttcta
    15061 agaatcctcc accaaacgct tcaagatttt tctcaatttc atcaaagcgc tctttgttca
    15121 aatcaatctc ttgaccaact ttataagttt ctttcgtgtg aacatcaatg aaaactttag
    15181 ctactttata tttggtcata gaggatcacc tacccttctg ctaatacagt tgcttggaac
    15241 acgttgtccg cttcggggaa gctaggaagt gcagtaccag atgcttttgt ccatgttcca
    15301 acagggtcaa gatttgattc ataaaccatt gcaaaaatgt ttccaacttg ataatcatta
    15361 gtaccaccag ataatagccg agattcttct ggcgttacac cgaaaataga ttcgcccgga
    15421 ttttcatcac cgaacatgac aagtttattt tctgggaagt agcgctcttt gaccaacacg
    15481 ccttgagcat tttctttata gtacttagca tcgtacgttg cgattattgg caaaccaaat
    15541 tgttgtaaca actgatttaa tgttccagct gttggcagca atccagcatc tttgaagtaa
    15601 gctttgatac cagcattttg caaaatagca ttacgaactt tagtcgaagt taagatccga
    15661 gttggtgtgg tatctagcgt acctgcccat gtagttaata atccgataac atcggtagaa
    15721 gaagcagcga aatcaacggt agctttgtgt tctgacggaa caccgtaatc aacaactaag
    15781 tccaatccgt tttcatctaa tgttacggtt ccgtttgcca atacttccat gcgcattttt
    15841 tcaacacgcg cattaacaga agaaaccatt gaaaatacat cattatatac ttcattttcc
    15901 aagaaagcct gttcttcagc agtacgagga ttgcgcaatg caatcaagtc tttttccttc
    15961 aactgaattt tgcgtttgat gaaagctaat tcctgagcgc tgcgagaagc tacacgagat
    16021 gcaatttctg cctcagtatc aaatgcatgt acgcttgcga tcgttggaat acgagtacct
    16081 gcttttagaa tgtcaaattc aagtccttgc acctttcgtg ctggaaaaag tgtttcaccc
    16141 aacaaagggg gagcttgacg attgtttaca tagtctaaga cgttacgttg tgaaaataat
    16201 tctgcgatat ttgccattta ttttttcctc ctatattcct attacagtcc gcttgcagcg
    16261 gctggtaaag cgatcatttt cccagatgca tcatataatt tgatttcccg cattgctttt
    16321 tgagctgcat cgcttggctt aaccggcaac cgttcaatta gaatgtgtcc atcaataata
    16381 acgcctactg gttgagcgcc gttactaaca tcaacatcat taattgtgat gccttcagct
    16441 ttcccatcgt ttgctgggta aactgaccct gctggcaata caccatttac aacacctgcg
    16501 tgagtgtggt ctacttgttt tgtgaatgaa atgaattgtt gagattttag aaaattgatt
    16561 tctttgaatg tttctgattt tttaacataa accattgata ttcctcctat ttaattgccc
    16621 aagggtcatt ctcaggcttt ttcgtttggt tgtttgcatt ttttgctagc tgtgcaccac
    16681 gagaaattgt tgcgccgttt ccatctaaag gaacttttcc gccgagtcgt ttttcatatt
    16741 ccgctttgat tgcttcacgt tctgcttcaa cagatgctag atatgtttta acgttgtttg
    16801 atgtggtttc agcatcttct gacacaatta gtcgaagcat ttctttcgtg ggcgtagcgc
    16861 ctttctcaga cagcatttca cttgcttgtt ctgacatctt agacagcact tctttacgtt
    16921 cgaattcagc tagttttgct tcaagttgct gtttctcgta atctgctttc tctttatcgt
    16981 ccatttcggc aagtttggca gcttcgtctt tttctgcacg ccatttttct tcagcagcag
    17041 tgacagcttt ctttgtttca gcagcgatca ttttcgctac ttcatcacga gaaaatgttt
    17101 tgccagtagt gttttcttct tttgcttcgg gcggtgtctc ttgtgagcca gctggtaggt
    17161 ttccttgttg tccctcatca ccagatccac cgtctcctgg ttcagaaaaa aattgtaagc
    17221 tcattggcat aaataaacgt tttttcatga ttaatcctcc acggttacgc cgctacccga
    17281 taatttaact agttacgcca gtcagtcgga acagctttct ctttagcgcc tgtaagcagt
    17341 aagaaggcat aataaaaagc cgttatttgg ctccgttaat atcgcctgaa ggcaaatatt
    17401 tcacactgat atcaaacgat ttaataccgt cttcccattc actatcaatt tcaactgaga
    17461 ctcttagcac tttttcgttt tgcagaatct gatttaagct tgcgatagct ctaacaattg
    17521 cttcttcgtt ttccatatct ggcatctcct ctggatcata ataaaaagcc gttagctaat
    17581 gggctagcga ctttgtaaca tgctctgatt catattgttt tgcaaagctt tgaagtactc
    17641 tgggttagac tgaaccaatt cggttattga ttgcacgaca ggttcaataa cctttctagc
    17701 agcttcatat acttctttta cgattggttc taagacatca attaagtgct gcttaacggc
    17761 atcatagtct tctttaaatt gagcatcgtt ttgatacaaa tgcaaaattt attctttgct
    17821 ttttccgtat gctgtttcta aaaatctgtc agatataact tgaaatgaat ctttctccat
    17881 ttttataacc ctctttcttt aagtgacttc tcaaaagctt ctctgtcaac ataaggtgcc
    17941 gtactacatc tgcaaaatgg gtgcattggc gcacaattta cacccggcga catatctttc
    18001 aaatcaaata ctttcccatt tagaggcaga cagtacctac aagctgttgg ttcagatata
    18061 aatacgaact gatcaatatc agcatcacga tagctttgct cttgaatccc cgtctgcacc
    18121 cttgtagttt ccgtaaccat caaccgttgt gtattaaact tagtattctc tcgtccttct
    18181 tctgttagaa acttactcaa ttctggagcc agctgtttag ggtttctacc catcgtcaca
    18241 ctacgtacaa gcaacttatc taaatcagct ttcaactctg cttggtacat ccatagtcgt
    18301 tcgctaaacg ttgcaaatcc atctgctcga aacgagctgt ttatcacttg ctccactagt
    18361 ttggcataac cgcttttagc gatcgtcatt tctaggatgc ctgcttgacg ttgcagttct
    18421 ttcaaaccag cgctggtaag ctctcccgag aagtatttat ccatatcatt aaaagtggct
    18481 atcagctcaa gtccaatatt tgctttaagc aattccaagc ggttaacacg catcgttagg
    18541 ttgtatagtt tcaactcctt gtttgctgta ggtgagaagt ctttctcttt aacatacttc
    18601 ttagccttgc gagcaaatgc ttttacatcc atttcatcag cacgcttcat tgcttcgcta
    18661 cgagtgattt tctgcccgtt ggaaaaacta tcccactgtg cgtctatctc tttctgtatc
    18721 gcatcctgtg cgtattgcag acgcttcttg atctcgttca tgcgtttctt gtcatcttta
    18781 atctgttgct cttgccaagc tttctcccgc ttgatgaagt aatcttgtga tttcacttaa
    18841 tcactcctcg atgatttcgt aggtctcaag aaagatatct ggtttgcacg gataaaattc
    18901 accatgaacg cctttgatga tgtaatcacc atcagatatt tccatcgtgc cttctaatgt
    18961 ttcaatacca ggtaatccat tcggcatatt attatgatca acccatgctt caccaacgaa
    19021 ttctttgcat ttgcgatatg aacgtgcaga tagcatttta agttgcacaa tctcgacagt
    19081 aactggtttt ttacgtgctt tcatttctta tcctccctac caagaaaatc tgactaactc
    19141 aattttcgca tcaatcgtat gcttatcctc gtaatcctca acagtaaagc cgccatcttg
    19201 aaactctttg cgaatatcat ctgtgataac gtctttgccg tacaggactt cgttcctgcc
    19261 aatcttcata gcttcagcaa tagcatcttt gattttttcg ctatccttct tctgatactg
    19321 gttcatcatc tgttctttga gattcatctt cttcaacctc cgtatcagtt tctttgtcac
    19381 tgtcaaacac accagaaacg gcttgtttct tcaatcgctt aagttcttct tcaaacggaa
    19441 cgccagttaa ccgctcggcc atttcgcaca acgtttgatc tgatacgatg ccgaccattc
    19501 cagcgattac gctcatgatt tcttcgtcag attgcggtac gtttggcgta aattgaattt
    19561 gtatctcgtt tactttgttg tatagctgct cttgttgctt ttcatcagaa acaaaaaagg
    19621 ctttgactgt atcaatcaag cctcgtggtt tatccagtcc gtctttaatg ctccaagagt
    19681 gtgtaagcaa ccgcagacgg cgcataatag ctttcttaac cattcgttcc ttgttcttac
    19741 gatcgttgtc tgaaccccaa cctttgaaac ggaatccgat gcctgactgg ttggacccga
    19801 tgttctcgtc agtaaaatca ataagagatg tgaagcgtaa gatatcagca actgtccggc
    19861 tgtcgttagc ttccattcct gcaacgtcat actctttctt aaggtaatac gcatcaggtt
    19921 ctgcacctgc aacattgttg tcgtatatct ttttatcgcc taatacaagc attcttgctt
    19981 gcatcatagc ttggaatact gctaacttgc tgttgtcgcc ttcttggtcg tctgctgtgt
    20041 cagggttccc tttaatcacc aagtaggctt ccgacgaatc ttgttggaag ttagccattt
    20101 ccgatcgtga aaggtcgtat gcatcaatgg aatccattac atgctcaaaa tcacttgtgc
    20161 gctcttcgtt gttgatccat tcattgatct gaacagtgtc aaaatagctt tgaacttctc
    20221 catcttcatc cagtacggca ttttccaaat catcatcttt agctgtgaag taatagttgt
    20281 atccacagtt tgtgtataac tcgatccgag tgaatgactt atccaaaaat ttctcaacga
    20341 agtaatgtac gccgcaaatc gagtttctat cttttgtgtt gtcataaatc acaaatgttt
    20401 gctccgcatc aaacttagct attgtttctt tgccatattc gtcacgtcca acccactcat
    20461 aagcacgacc taagccaaat gcatcacggc tcatcaactg attatggtag tcctcattag
    20521 attcgctagc aaaccgatta attcgttcaa caataacctt atcgccgtta taactaatcg
    20581 gattacctaa caagacacct tgtttgaacg acacaataaa gttagcaaaa tcgctcgcta
    20641 tgcggttgtc tgctctcccg tctggcttgt ttggacgtcg cttaatgtta ttgtctgcat
    20701 tcagatatcg ctttagctct tttaatctag gtacttgtct ttcacgatga tgcttgataa
    20761 agccaacaat cattttccac atatcttcat gcttgaagtt gataaactcg ctaacctctc
    20821 ctgtctgttg atctaacatg tcgattttcg gcaactgact aactggcacc ttatagacaa
    20881 ggttagcttc ttcatcgaat cgttgttgcc ctagaagctg aatattctgt tccactgtat
    20941 cacctctaca atcctagttt tttgaatgtg tcgatcgtct ttttgacgtt gactggttct
    21001 ggttgctttc tcatgtcatc gttaaaagca tatcgagtag catcgattgt gtgattatct
    21061 ttgtcctcaa gtcgaggttt tggatttcca tcacgatctg tttgatagtc gatgttttcg
    21121 aactctttag cgatgtttgg tgttctgatc ggatcgatgt atatcgcatc taaatcatct
    21181 agccattttt caccgtattc aacactatca ggccctttag taacttgcct tatcttcgat
    21241 atgtcatgct catttctcat ctcagcgttt gatttaggtt caacctctgc gatgatgtcc
    21301 tggtagtgat acttattgct cttaatccat tgagctgcac gacgattgct acatttaacc
    21361 tcgtatagtt catcaatggc atatatgcaa ttcctcttct tgtcataatg ccaacgcaca
    21421 aaagcaagag gatctgttgc gtaaccgtag tcaagccctt ggcgaatgtt atcgaagttt
    21481 gccaccattt catctgtgat acttccagct tcaattcgta gattgtcgaa cggtacaaca
    21541 ccagatccaa tagctttacc atcatactcc cactcagctc gtctagggtt ccttgctctg
    21601 gctgcttcga cttcgctcag aaactcctta gaaataaaag ggttatcttt ataagttgaa
    21661 tgatgaatga atgtattctt aggctgaaag gatgtttcat attttttgtt tacccatgat
    21721 tgtttccgtt ttggcggatt ataactgtaa aagaatttat aaaaaagacc atcgtccaac
    21781 tctccacgta aaagggagtt cgtgatagtc gtgacttcat cttctgtttt aaattctgct
    21841 aattcttcaa tccaacctat agcaaatgga aacttgctat cttttaaaga cttgattcgt
    21901 tctgggtttt gagcaccacg aaatatcata taattccctc ttggaagata agtaatttta
    21961 agtggtgact tattaaactt gaataggtgg gaaacacctt gctgttcaat cgcccatttc
    22021 atctgttcat acaatgattg ctcaagtgta ttatcaacat aacgtatacc tacagcattc
    22081 actgcatatc tcataagtaa ctgagtaacg atatgcgcta tatccgatga ttttcctgaa
    22141 ccacgcccac ctttacaaac tatattaaga atatcttgat ttaaagttgc tctccatact
    22201 gaatgaaact tcggcggaag aaattcagat agttttttag ccatcatcat cactacttat
    22261 atcatcaatg aaagtaggta tttcagaaat ttcaactttc tgcttatcta caaatgctgc
    22321 gtttatttta taataatgtt caagtgcttg gttacgttct ttgaaacctg ctgaatattc
    22381 actcacttca cgttctatga tttcgtttgt ataaggatct ctcttaacaa cttcaaagcg
    22441 ttgtggttct ccttttgcaa tagaagcagt aatagccaaa gcttcttcca ttgttaaatg
    22501 cctcttagtt tgaacttctt ttagcttctc ttgaatgtag tcggatactt ttccaccttt
    22561 ttccaccaat ttttcttgtg cgttcttagc gtagttttct ttatagccag ctttcagtgc
    22621 tgactgataa gcattgcctg tgatgatgta ctcatcagca aaggcttgtt gcttaggatt
    22681 caacttactc attttccatc accacctttg ttatatgtta atgatatttt tttatatttg
    22741 tctgtatgtt ctgctcacta aaatatccat gaccgcaata aataagtttg catttatcaa
    22801 tttcatttgg cgtagcttct ctggtcattt caacaatgga gtacttcttt ttaatctgga
    22861 ctgattggac aactctaatt ggatcatctg cgtttggttg cggatattta tttgataatg
    22921 atacatacca gtagttcctc attatgcagt ctcctttacg caaaataaaa agaccactca
    22981 acgagtgatc taatatgtaa aaactacatc tcaacaatga gatgcagtta tagctctatc
    23041 aagcaaccta caccgattcc atcgattact atcgacctcg ccttgctcgt gtactttgag
    23101 cgcccatttc caaccctcgg ttgctaaaat cactggcaag gaatcgaacc ttgcaacacc
    23161 attttccgat attttacaga ctctccatca taaaaactct cacctctaga attgtctatc
    23221 tttaggggac ttatttcaag atacctcttg ccctttaccc tcgacggtct cttgcctagc
    23281 tgcataaact aagctcaggt gttcattacc actgatttaa gttattagcg ttacctttcc
    23341 gccacagtga caaattaata ttgtgaaaat aaatactaag cgtataattt tagttatcag
    23401 cgagtggtcc gctgaaataa atcaaaaggt ggtgaaaaat ttgtactttg ttataagaga
    23461 agcaactaat ggccaatatt acttcgttat taagtccaat aacaatgaag tagtagcaac
    23521 tagcgaaaca tacttgacta aatattccgc tgaacaaacc attaattcta taaaaaacgg
    23581 aatcaccgaa gactctcaag ttatcgatat gactaaataa gacgactaac ttcatttgct
    23641 agggagttca tttcagctgc tttttctacc aaagcagttg agatactttc tagcttttct
    23701 gatttaacag aattaaatac tggcgtcttg atactaatcg atacatcttg acgaggatca
    23761 ggactcgttg ctctattttt ttgcaccact gatctgtact tttctactga atgatactca
    23821 attacttgtt ctaaatgttt tttgtactct ttacatacag cttctttttg gatcaattct
    23881 aaacgttgtt gctcaataat atcaatcaac cgttttcgat ccataccttg atactttatt
    23941 tgttcgactt ggtcactagc cttgttgagc agttctttta gttcagtcaa atctaatttg
    24001 acagttactt tgatttcttt atccattcgt ttttcctcca atacataaat taatagacag
    24061 caacaaaata acattgcttt gataatttgg tatcgaccac tataaatttc ttttcttgca
    24121 attattttta atatatgcta gattatcaac cgatatagtc actgcctgta ctagcggaaa
    24181 ctagtgcagg ttttttgttc tatttactca aaagttatta cgataaattc ttattgtgaa
    24241 aataaatact aagcgtataa ttttatttat cagcgagtgg tccgctgaaa tataaaacaa
    24301 ggatgtgcaa gacaacatgt ataaacccta catgattagt tatgatctta ataacccagg
    24361 gcaaaagtat gataaagtat ttgaaataat aaaagaattt ggggcatata taaagctaca
    24421 aaaatctttt tggttagtta aaactaattt gaatccaaat caaatgtgtg aaaaattaaa
    24481 tacagtactt gataataacg attccttatt catctgtgaa ctgcaaaaaa attatcaagg
    24541 tagagctaca gaggaaaatt ggaaattcat taacgaacat attttctctt agtaaggatt
    24601 agatattttt tctctgttta aacaattgca aataccgtca atatctgaat tagaaacact
    24661 taccttttgc tccttgctac taccaatagc ttggagcaat tcttgtattt cttctggagt
    24721 gccttctact gataatttca tttttcttcc ctccaataca taaattaata gacagcagca
    24781 gttgaaagac gacaagaaca tgtttagttt tgtgagtgct gctgtctaat caaaataaac
    24841 agcaaccgat gaagaattta ggaggagttg aattcacatc cttttcttca taattagttg
    24901 ctgtctatct aagaagaagt ttaaaacgat gagggagatt gcctcccttc gtttattttg
    24961 tcgatcctgt ttcctaatct ttcgacacta ccataataac actggtaaat gtctaaaaac
    25021 cgccatcatt ccgccaaaaa accgccaaat tatttataag caattatttt tccgtgttta
    25081 tatgcttctg caaactctat tagagcttcc gacttcatcc gttgtatact tctttctgaa
    25141 taacccactt cacggctaat tctgtagttt gagaagctat ctggcacaca gaagctgtag
    25201 tagagtatct gacgactaat cagactaaga gccatcaaag ccgctagaat cgcatctctc
    25261 tccgtttcta tatccatcat ctgaataatc gcgtcttctg ccttattgcc atgcttcggt
    25321 gccttcggca tatccgttat aatcggcgac ttaatatcta tcaaagagcg acctgccatc
    25381 cgctccaaac gccgaaagtt cttcagcaca tctctcgcat tacatcttgt ctgtttgaaa
    25441 tctacctctc gtaacaattg catcaagtca aaccgctcct ttatgtgata taataaatgt
    25501 gttggattta ttgaatcagt cggagcgatc cggctttttt tatttgctct tgattacttc
    25561 catgtcaact aatctcgcta cagctaaata atgcttgtgt tttgcagtcg aacgatcaca
    25621 cttcattgta ttttcaatac gaatgattgc tgagtttgac agtatctttt taacgtatcc
    25681 tctgaacgga taaacgaact cttctgcttc acagcggacc atgtcaccga ctttgaattt
    25741 tgatttcttg cgtgttttag ggttctttgt cggcatatct agcattaaac cgccgatacc
    25801 gtgactgcta gcgtaaaatc cgtcttttag tttcatcttt ctaccacctc ttccactgtt
    25861 ccattcacca acaatgctgc agcatccgct gttgctttat cagtgaaaag caatcgatga
    25921 gatggattgc attcaaaact ataatttact ccatcttttc cccataaagc acatttagta
    25981 agatagtttc catcggcatt ctttattgcc cataacggct ctttctcaac ctcgtagccg
    26041 ttgtataggc tcaatagtgt ttcatatgat tgtcttgttg cccaagcgag taattcttct
    26101 ccttgctctt gcgtcactat gccatctcgt acaaaccaat cacagaaata atatgaatca
    26161 ccatctttag atttgattaa atacgctatt ttttcagctt ttgtaaaagg gtccgtagat
    26221 ttctcaagcc aaccggccac gagttgtgga ataactggtt tctgcggttc gtctattttt
    26281 ttcattaacg taatacctga atttatactc tcgttatagc aagcagctat tgcatcattt
    26341 cctatcgctt taatactttc taactttttg attgcttcct gtttattcat cgctgttcct
    26401 ccaataactc gctattctcg tatatatttc cggtgacttc gtacgtgtat tcttcaaaca
    26461 gctctgtgtt aaaaatttga tactctaagt cttcatcaac agtggcacaa actaatccgg
    26521 aataatctat tgaatttttg acaatacaga ctttattatc taagtaatcg aagccatttc
    26581 gcacgctgac taatactata tccccttcaa atatatccac accattctta tctttcattc
    26641 ctgtggattg catgaggtcg atgtttcgca cgtgatatga tttcaattcg gtaggattta
    26701 tccaatattc aataaagttt gttttgccgt ttttagtaaa atgcaagacg gctacatctc
    26761 tcattacgtt ctttcgttta tcccacgctc taaacttcgg tatcattcgc tgtcctcctc
    26821 gtatttttca atcaattcca ttactttttt cactatttca aactcaaccg cttttgattc
    26881 ttcgaaatca tgaacaattt ctggaaacag tacatcatca actacccaca aaataatctg
    26941 gcgctttcca ccaaaattta tgattagatg gtccgattcc acagatagtg ttgctcctga
    27001 ttcgatatca tataaatcca tgctgaattg aacgagtttt tttatcattt gctgtcctcc
    27061 tcaaatactc ttctaatatt tctttatact tctctacaaa tttgaaacga tcttgatgaa
    27121 gtttcttgct ccaatttgtt tgccgatcca gctcacgcat ctgatcgaac cctttttgaa
    27181 tttcgttgta ataaaattca atgtttgctg ctgctttcca atgcctgcta cttcgcactc
    27241 ctgctcctgt ttcagccatt tctaacttaa ctaattcagc tcgttctttt gattttttat
    27301 cgttctgaat cttcatcatg attttcttga ggatgatgtc actgtattgt gtaatgagat
    27361 ccattatctc tcctccacat acctaaactg tcgtcctttt gaatcaatcc ataagctcct
    27421 agctctatcc cagataatgt ttttgcttaa tccagtaatt tcagataact gttcagcagt
    27481 acctgttact aaaattcggt catcatgcca gattgcaatt tttctcggcg ttctccgttt
    27541 gggcttttca gtccacattg atttaccgag cttttggact tctgcaacta tttctttgtc
    27601 ttcttgccaa gattctgact tggttaattc agcaattctt ttcattgctg ctttcttatc
    27661 cacgctcatt cctccaatcg atggatttcc cttcttaaat tctctatgtg caaatcgatt
    27721 gcctttctcg ccgtttcatt gaccatcact gcctttgttc gttccagatc gtcaatctca
    27781 cgctgaaggc ttcgaatacg catttgaatc acttcttctg ttgtcatgat gattcctcca
    27841 cgtatctaaa cgttctcttc ttagcgtctg tgtagccaca ccttgctctt ttcctcacga
    27901 ttttctcgtg caagcctgtg agacttgcta actgttcagc tgtccctatc actagaagtt
    27961 tgtcaccgtg ccaaattgcg atttttcgtg gtcttggctt gttgctcttg tctgcccaca
    28021 tcgctcttcc aagcctcatc acttctgaag cagcttcttt gtcattttgc caatcttctg
    28081 aataagtcaa ttcgataatt cgctgcattg ccgctttctt atccatcccg acattctcct
    28141 ttcagtaatt tgagtacttg atcaagtgcg ctctcacgtc cgccatggaa cgtgttgagc
    28201 cacttgtctt cgtacgatac actttgtctt aaagcttctt gatgcattag ttcaatctgt
    28261 gctgtaaatg tctttaggtc catctgatta cacctgctca agttcattaa gatgtttttg
    28321 caagccttta acacaatcaa caaatagtaa tttggtatat gctaaatttc ttaattgtgt
    28381 tgtatcgata taaagtgcga aatagtatct gagcttactc caactcgaac gatcattctt
    28441 aattccttca attccagctt cttcgagttg atcatatacg tctctcagga cgtctaattc
    28501 ctcgcccttt ttataattcg ctatttcatt aattagttct agataatcga tcttcatttt
    28561 tccacctctt agaatggtgc ttttgattgt ctattagctc gttctagcgc ttttttcttg
    28621 agataggctt cttggtcgat tgcccattca ggaagcttct ctcgtcttcc tgtgcgcttg
    28681 tatccactgc ttgcgttctt aggttcactt ttttctttcc ttgcccaact tcgaatagtt
    28741 gccaaatagt ttttataagt cttaccagat gattcacaat actctgacag tcgttcgatt
    28801 cgctcttggt aatcattagg gaattctgtt ttgagtttct ccatctgctc atctgacaaa
    28861 agaacatttt tatactctcc gtatttatga cggacgggct tagccttcga ttttttcgaa
    28921 ggcggtaact ctcttatata ttcttttgta ttattaaatg tattattaat agatgtatta
    28981 ttatctttga ctttttcgtc aataggggta ttgcgttttt cgtcaatagg gtattgatta
    29041 attcgtaggt acctattgat tatttgattg gtaccctctt tgtaaatgat ttcccgattc
    29101 aagtatccaa acttaatcaa atcacttacc catcgcgata tggtctcttt attcacacca
    29161 tataaatctg caaagtactc attgcctgcc caacaaaagc ctctttcatt acacaaggcc
    29221 gttatctctc cgtataacaa cttagtattt ggtttaagtc ttttgtcgta ccttacgttg
    29281 gctggtataa tcgcataata acttcgatgt tctgtcattt ttaccctcca atatttaact
    29341 ttttgattgt tttctggttt aatttgatcc ctttgatttg atatttattt ttgaaattaa
    29401 tcacacctat tttgtgcttc tctgtgtgat ggattctgca gagtgctgca aatgtgtact
    29461 ctgcatgatc aacttctttg cgctttcgtc ttcctagcgc tttgtcaaag tgatcgatgt
    29521 cagctcctgt tttgccacag atgcagcaga ctctttttgt gatgcatttg tagaagtaat
    29581 attcttgatt cgcaggtaaa atctcatagc cttctttgaa aggaatatga tgttcaaaga
    29641 tgaaatctaa gatgatattt gctaagacat tggcatcact cacagttgta ttcgattcgt
    29701 ctttcaggct tattttgcgc cctgtgacac cttcaaaacg aaagtagaag aattccttcc
    29761 agaagtccgt tggcatgcct gtatcgataa aaatatcgcc tatcagcgca tagatgaagt
    29821 ttcgttgctg tacggtgaaa cgtctaggat caataaaacg aatttcaatg actcgatcac
    29881 catcgtagcc gtcatacatc gtctttagtc gatcaatgtt cacttcctca ttgatggttg
    29941 cgcttatgtc ttttcctttg aactttttca gaaccgctga atatgaatcg attaatggtt
    30001 taaacactca tatcacttct tatctaattc ttttctctta gctgctattg ctcgctccat
    30061 caaggcacat tgctcatagc ttaactgttc aatagtttca acgttatcag ctaagagccc
    30121 taatttatct gtctgctcat taacatattc gattaaggtt ttggtcatat ctttacccat
    30181 ctgctcattg aaagcttcta gaatcgtctc tagcatgttt aatttctttg tatcgattct
    30241 aggtggtgtt ggaatatctt ccccttgaaa tacatataat cccagtccgt gtagagccaa
    30301 tgctttcaca aagcatcgct tcaatgagtt attgatttgc attgcatttg gtttaacaac
    30361 tggttggttt cgataatcta aaacaggaaa taattcggtt tccgtgtgtc ctttaaccgt
    30421 tactgagaca gatacataag tcccagtttc atccataaga aaaggtttat attcctcaac
    30481 aagaaagtct tgatgagttc cagaaacaac cctgtagtgt ttgtactcat taatagttac
    30541 cgttgcctgt ggatcattct ttttcataat ctcccacgcg tgagcccaag ataaataatc
    30601 aaaatttcct tttttcttga gaattttatt taacttacga ctaaaaagtt tttcaaagtt
    30661 cgttgtccct ttgatttcac tcatcaaatt ctgcctccat ttcagcaatg tatttcttac
    30721 ctggtccgta ataagagata tcaatcaagt tatctctgtc gtactcttct agcgcatcaa
    30781 tcaagccatc ttcgatgacg taaatatatt caggtttatt cgaatgcttc gatagatgga
    30841 taagataaac atgatcccaa atagttacat ggttgcctaa atcatcttga tcccaagcta
    30901 gttcttcatt cgtcaaaaga tttcgtctga tttttcgacc acttgtttcc tcaattttcg
    30961 gcttgcccca atcaggatca atcaaatatt gatctagagt ggaaagttct ttttccatat
    31021 gctaaaatct ccttatgatg tgttttcttt gtgactcttt gcttgccggc ggagtcactt
    31081 ttttatttgt tgccatgctt tttgcttgtc aatatgttgt tggcttagga tgattggttt
    31141 atagtatttc caccagcaat tagcaattgc cgtccctatt cttagcgctt cagctctatt
    31201 cattgtcatc accaaaaagt ctctgttgtc tgttcagttg atcaatttcc atacggatcg
    31261 cagtttctgg caaccacatt tcaataaatg aaacagcatc atcgaatctc ttacgaggta
    31321 actcgccata tcttgggatt gaaaaggtac gtttaaattc agaccaaaat tttgagaata
    31381 cttttttgct gatttcttca taagctcggc tttcttttcc ccctagaact cccataactt
    31441 tcatatttcc tttttgctta atttcaaact cttgttgtcc gctaattcgc atagtatctt
    31501 taagcatgga aacatctttt ttaacatctt tcatttcttc taattgatag atcatcatat
    31561 cttcaattgt ttgaggaaca gtattcttgc gaataacatc ttccatttcg ttgaatgctt
    31621 caatgtattt ttgtttgaag tagatagctt tctttcctgt aaaccccata gccagcaaga
    31681 aaaatccatc tctactaatg aagaaaactc gtcgatttct gccatatgaa tctggttcat
    31741 taccttctac aaacatctgt ccaaaattgg acacatcttt ttttagtgca tcaatatctc
    31801 ttaaaacatg ttgatgtttt ttctcgaagc tttctgccac ttgtaagctc gtagtcacag
    31861 cttctttatt tttcaaaatt actaattctt gcattatttc ttctctcctt tttggtataa
    31921 tttagttaaa aactggtggt gtttattttg aatttttttc acttaacaat ttttcaatgg
    31981 atgtcaataa taagttttat attgagtacg tttttaacag ggataaaaat aaaaggaatg
    32041 cgccctcaac tagaagttga acttaatgct tcttacttcg ctgcagatat gatttataca
    32101 aaagtaatca tttctaacta ttcaacagaa ccagctatgt tagttaattt agaactttca
    32161 tccgctaact taaaccgcag atggtcagct actccattta agaaattaat tgctaaagga
    32221 ggatcagtcg atgataatag aatatactct gaagctgtac cgctaaatat tcctcctaaa
    32281 agtgctatat cttgttacct tgcatttgaa gtagggaaaa ttaattttaa taaactcctt
    32341 aatcaccaaa caaaaatgat atttactcta aatagaaccc aaatccacaa aattgttgat
    32401 atcaaaaata caaacttccc aatagagaaa ttagtgaaag aattaaactg agtaatagta
    32461 atatacattc tagtttctct tttctattca tttttagtca gccccctcgg ttggcttttt
    32521 cgctctgtac tcagcttcag cttcatcaag ccccataaaa atccaaacca tgtaaacaat
    32581 cgttcctatc aacgcttgcc tgcttcccca aagtcctaaa gcgtagatga ttagcggtgc
    32641 gctgaacact agcgctctat taaattttcc catccgcttg cctccttaag atttccgaaa
    32701 aatttgtttc taaaaattca agtgttttac ttcttaaaaa tagatatgtg tctcttcctt
    32761 caactggata atagacaaat ccatttttgt ttttctcgat atcgataata tttctatatc
    32821 ttgggttctt taaaactcta gaagtaaacc aatcatattt tctgttaatc cgttctagca
    32881 cttctggcaa cgtcatccat ctaccagtat catcagcttt ttttaactcc tcataatcca
    32941 cttgggagat aattacatag ccttctggaa ttgggatttt tgcttctaga tattgcatta
    33001 gctgttcctc cctttacaat tcgtacatag tgataatcga atctataatt ctgtttgctt
    33061 ctgcagaagt ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc
    33121 tttcagcaag catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt
    33181 ctctatctcg ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat
    33241 ttcctctaaa tccatttgag ggtaatatcc ttcttttttt agtaattcgt agataaatag
    33301 acgccctttc tgtgtccatt tggtattcat tacaatttta gtgccaccat cggctttcgg
    33361 gatctcagtt gtatgagatt ttgtgtatcc ttggttcatg tgttttttgc acaataacca
    33421 ttggttaccg acttttttct gaatacctag tttatgaagt aatttattca tctgttgtgg
    33481 agacatccca taatctgctg caatctgact aattgttact gaatctgtag aagataatat
    33541 gctatctaaa taggagattt taggttcgta ctctgcaatc ttttgttctg cgattaatct
    33601 tccagttctt tcttctttta gttgagttgc taattgaatg attgtatctg gattaagcaa
    33661 agcttcttct actttttctg gagttagata acctccatgt tttctaattg ctggcaacac
    33721 ttcacttgtt acccaacgtt taaatttttt ggcagaggga agttttgatt ttaagattaa
    33781 actgtacaaa ccagattcgt tgatgattgt catctctctt gattgacctg aggtcgtgat
    33841 tcgcgacccc atcttatctt ccaaatctac atgccgcgac aaagcatctt tagtgtttga
    33901 ataacccaaa acactcgcaa cgtctttgcc tacaaaatat ggttcatcat ttactaaaat
    33961 tgtccgaact tcgttttgtt cgaaattaaa aatttgcggt gtgttcatat ttctcattcc
    34021 tttcttttat attcgtaaac aaatttaaca acttttttct aaattcaatt gacagtatct
    34081 gagtaatact ctatactaag agcataatta aataagacat aaaaacattg attttaaagc
    34141 tttcttggcg gtctgcattt acatatcaat agtgtttttt gttgtctttt tagttgttaa
    34201 atctgtttac aagatatagt atagctcatt actcagaatt agtcaaatta tttctgcgta
    34261 tttttctaaa ctttttttgt aaacatttag aaaggttgtt ttatcgatga atacctatga
    34321 aataataaaa gacttggcga agaggaaaaa gatatctata cggcagttag aaattaattt
    34381 tggatattct aacggttatc tagcaaagtg gaaaaataat actcctaatt cagatgaatt
    34441 aagtcggatt gctgactatt tcgatgtctc tgtagactac cttctaggta gagaagaaag
    34501 agaaacccct aaacatgtgg atttatcaga agacgatact gttttttctt ttgatggaaa
    34561 agaaatatct aaggagacaa tgcgtaaagc gattgcaatt gctaaagctt tagaggaaaa
    34621 tgaatagttg gagtgatggt ttgtatgtat ttgaaattga aagaaatgtt gagcgaatac
    34681 aatttgaaag tagtttatat ggagatgaaa gagcctggtt tctactatcc aaaacccaga
    34741 atcatttttt tgaatgaaaa tctatacggt gaaactgcag aagcctttca tttatctcat
    34801 gaacttgcac atttcagcgc ctctcatttt gaattttcag ttttgtacga tacttctaca
    34861 acttttcact caaaatttga aacagaagca gataaaatag ctattctaat tttattaaat
    34921 atctatattg agaacgaact gactgatgaa tctcagttta atctcgaaaa atttatggag
    34981 tattattcta ttcagaataa gctcagatac acttgttatg ctgtctgcca atgctatttt
    35041 aagaaaaaat attcttacgc aaggcaatat gtatgaatac atcaagaatt attaaaaaaa
    35101 ccgatttcag caaaacatta aaaaagcccg tgctgcaaca cgggctcttt cctcatatat
    35161 gagcttctac aaaaaaaatc atatcacaga aatgaggaag aaaaaatgga aaaagagatt
    35221 ttaataaaat ctccaggaaa aaccttgatt aaagtaactg atgattctat ttcaattatt
    35281 agaaaaggat ttataaattt agttaatcaa ggtattaaag gagaaaaaac tatccccttt
    35341 aaaaatatat cagccgtaca attaaaaaaa ccaggtatga gtaatggtta tatacaattt
    35401 accttgctag gcggaaacga aagtcgcgga ggtatattag cggccacgaa agatgaaaac
    35461 accgttatgt ttacaaaaaa atattggaat gaaatggaaa atttaaaaaa atatatagag
    35521 aagcaacaat ctatattaga taataattcc aaagaaacac aaatttcaag tgcagatgaa
    35581 attaaaaagt ataaagagtt gttagatgaa gggattatta atcaagaaga attcgatttt
    35641 aagaaaaaag aattgcttgg tttataatat tcaatatcaa aagagaaaga ggatctatta
    35701 atggacggta tatttggttt tactggtatt gttttattta ttataggttt aatcatgtta
    35761 attgtaagat ttatcaaaaa aacaaataag aaaacaccta tgattctcct tattatcggg
    35821 attattttta ctaccgtagg tttctcgctt tcctcatcaa acgaggcaaa tgacaataac
    35881 aaacaggaaa gctcatcatc tgatactcaa actactacat ctaacagcag caataaaagc
    35941 ctaactcaat ttgaagaatt tttagagaca aataatcatg actggggtgc tttcttagat
    36001 agttattatt ctattactcc agctactgag caaaatacag cttttactaa atatattagt
    36061 ggtaaaacat atacttttga aggaactgtt atcgaatcaa tgactactag aatagccatt
    36121 atagcagaca aagagtacga tgataaaagt tggaatgata tttctgcaac acctaaagtt
    36181 tcatatgtaa tttttgcgaa ggacttaaac aatgctgata cctttacaaa gggcgataaa
    36241 gtaactttta caggagaaat tagttcaaga ggatcgaaca tagaaaaatc ctatgctcaa
    36301 tgggatatga ttaatagtaa agtaagtaaa aaataaatga accacgcccc actaagagtt
    36361 agattgtgca caataataaa acctatacaa aagagtgcca tcaggagtca caatgcttgt
    36421 aaaggaaatg acaaaatagg aggtaactat ggataatagc aatgaaaaac taaccaaaaa
    36481 agaatatctt gaattaatag ttgaaggcgg tattggagca attccttata ttggtggcgt
    36541 tgtgcagtca ctttattttg ggtctaaaaa cgagaagcgt ttcaaacgca tcgagaaatt
    36601 ctacaaaaat ttaaacgaat cattagaaaa cattcgcgat caaattccag aagatgtttt
    36661 caacttagag aacaaggacc aactcattgg aatattcgaa gggataaatg acgaggtaga
    36721 aaaatcaaag gcacaaaata aaatagatat gtataaaaat ctttataaga attgtttact
    36781 tcgaattaac aacgcttcat gggataacga ggagtattat cttcttgtac tgaatcaatt
    36841 aacatcgatt gagattcaat tgttagcatg cttaatgagt cgtggaaacg aaaatttcac
    36901 tggaaatatc agcatgtctg gctattcaca agagctaata gatggtagct taaatagatt
    36961 atctgatttt ggtctgttag aaaaaacgat tagctccata gtattaggtg atgtcggcaa
    37021 acaaaatatg ggctacaaaa tatctcattt aggaattcaa tttattaact caactcttac
    37081 ttaagttttc tttctaatat gacatctaga cctatcagca atgcaacaat aaaaaaccag
    37141 acataccaat aatcaacaaa taatctaaac tctggaagtt cagttattct tttaaaaatc
    37201 actttaatca taataaacac ctctgattgg atgatctaat acacatccaa ttataccatt
    37261 aaaacgacaa atagcacatc cttgcgccgg caagcaaaaa ggatgtgcaa aaaataataa
    37321 aacccaaaca atgggcttct ttatagttcc tattgtatca gagaaagaga gctgattcaa
    37381 ttatggcaag attagtcaaa cgtggaaata gttggcaata cgaaatttca tacaaaaaag
    37441 atgacggaaa atacacgaag ataagaaaat caggatttaa gacaaaagga gaagcaaaag
    37501 atgccgccaa cgaattagaa tataacttga acaaaggcct taaaggggat cgcaaaaatc
    37561 tattattatc agattacttt gaggattgga tgcaacttta taaagaagga acagtatctc
    37621 ctatcactta tagaaaatac gaagatacgt taatgaacat aaagaaatat atgccagcgg
    37681 tattgatttc tgatttagat agagtgggat atcaacgctt tttaaataag tatgctaaag
    37741 accatgtaaa atctaccgtt attaagttta ataaccatat tagagcgtcg ttgaaagatg
    37801 ccgtagaaga aggattaatt ccgtttgatc caactagaaa agcagtaatc aaaggaaaag
    37861 attcattgaa gccaaaagaa gataaatatt tagattatga tcaatttaaa tctttaatga
    37921 aactcgtaga agaaaacctt tctgcgcagt actcttctcc tatgctcgtg ttagttgctg
    37981 gtgccactgg aatgcgattt gctgaacttc taggattaac atgggaagat atcgatttcg
    38041 aagatcaaat catcactatt aataaaacat ggaattataa attaaatgaa tggggaaaaa
    38101 caaaaaacga aacttcaaat aggaaaattt ccattgataa acatacgatt gatctcttaa
    38161 aaaagtttaa aatcaatcaa aaagaattat tcgagaattt tgaaatcaaa aaccctcata
    38221 attttgtttt tttcaactta aaaaatggat tagtttcatc aaacgccgtc agcaaatatt
    38281 tgcgcaaaaa attaaaagaa ttagggattg aaaagcaatt tactttgcat ggactaaggc
    38341 atacacatgc atctatttta ctttatcaag gagtaaatat acttagcgta tcgaaacgtt
    38401 taggacatag cagtttagaa actacaatgt ctacttatct tcatattgtt cgagagcttg
    38461 aagatcagga taaagaaaaa atcaatactg tgttcgatag tttatataaa aatgataact
    38521 agtttggcac atttttggca caaatacaaa acaaacccct gtaccgcaaa ggatacaggg
    38581 gttatttata cgttcctgac tggaattgaa ccagcgacct atcgcttagg aggcgattgc
    38641 tctatcctac tgagctacag gaacaatttc aataaatcaa ttgtagcttg tttgtttttt
    38701 acttgcaagc ttatctgcta aaaaaatcag cttataagtt aggcaaaacg tttccactag
    38761 tccttaacag atgataaact agaggtagaa tgaaataatg tagggaggtc ctttccaatg
    38821 aatgataagc caaaagaaaa agtatacgca gaacgcttag attcatctaa aaaaaatcgg
    38881 gaaaagatta cgaaagaagc aatccaagac cacaaaaaag aaaaccctaa aacaaaagaa
    38941 aacaaaaaat tcaaatttac gaaaaaagag gatttacttt aacattttcc ttcaaatgcc
    39001 aaatgaaaaa caaaagggtg gtgacaaaaa t
//
LOCUS       DACEKH010000017        37862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEKH010000017 DACEKH010000000
VERSION     DACEKH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.017"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(159..1469)
                     /locus_tag="IW662_000813"
     CDS             complement(159..1469)
                     /locus_tag="IW662_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0865657.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="IW662_000814"
     CDS             complement(1481..2899)
                     /locus_tag="IW662_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0865658.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4703)
                     /locus_tag="IW662_000815"
                     /pseudo
     CDS             complement(3139..4703)
                     /locus_tag="IW662_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
     gene            complement(4707..5117)
                     /locus_tag="IW662_000816"
     CDS             complement(4707..5117)
                     /locus_tag="IW662_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0865659.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5114..6037)
                     /locus_tag="IW662_000817"
     CDS             complement(5114..6037)
                     /locus_tag="IW662_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ0865660.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6034..6912)
                     /locus_tag="IW662_000818"
     CDS             complement(6034..6912)
                     /locus_tag="IW662_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0865661.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6899..8236)
                     /locus_tag="IW662_000819"
     CDS             complement(6899..8236)
                     /locus_tag="IW662_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0865662.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8239..9342)
                     /locus_tag="IW662_000820"
     CDS             complement(8239..9342)
                     /locus_tag="IW662_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0865663.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9416..10924)
                     /locus_tag="IW662_000821"
     CDS             complement(9416..10924)
                     /locus_tag="IW662_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0865664.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10890..11582)
                     /locus_tag="IW662_000822"
     CDS             complement(10890..11582)
                     /locus_tag="IW662_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0865665.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11604..12578)
                     /locus_tag="IW662_000823"
     CDS             complement(11604..12578)
                     /locus_tag="IW662_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0865666.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12878..13750
                     /locus_tag="IW662_000824"
     CDS             12878..13750
                     /locus_tag="IW662_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0865667.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14208..16406)
                     /locus_tag="IW662_000825"
     CDS             complement(14208..16406)
                     /locus_tag="IW662_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0865668.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16638..17630
                     /locus_tag="IW662_000826"
     CDS             16638..17630
                     /locus_tag="IW662_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0865669.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17690..19111
                     /locus_tag="IW662_000827"
     CDS             17690..19111
                     /locus_tag="IW662_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ0865670.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19104..20603
                     /locus_tag="IW662_000828"
     CDS             19104..20603
                     /locus_tag="IW662_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ0865671.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20642..22036
                     /gene="uxaC"
                     /locus_tag="IW662_000829"
     CDS             20642..22036
                     /gene="uxaC"
                     /locus_tag="IW662_000829"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0865672.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22195..22878
                     /locus_tag="IW662_000830"
     CDS             22195..22878
                     /locus_tag="IW662_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ0865673.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22880..23716
                     /locus_tag="IW662_000831"
     CDS             22880..23716
                     /locus_tag="IW662_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0865674.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23727..25481
                     /locus_tag="IW662_000832"
     CDS             23727..25481
                     /locus_tag="IW662_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865675.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25757..26098)
                     /locus_tag="IW662_000833"
     CDS             complement(25757..26098)
                     /locus_tag="IW662_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0865676.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26166..28337)
                     /locus_tag="IW662_000834"
     CDS             complement(26166..28337)
                     /locus_tag="IW662_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0865677.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28548..29405
                     /locus_tag="IW662_000835"
     CDS             28548..29405
                     /locus_tag="IW662_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0865678.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29476..30333)
                     /locus_tag="IW662_000836"
     CDS             complement(29476..30333)
                     /locus_tag="IW662_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0865679.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30318..33020)
                     /locus_tag="IW662_000837"
     CDS             complement(30318..33020)
                     /locus_tag="IW662_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0865680.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33033..34322)
                     /locus_tag="IW662_000838"
     CDS             complement(33033..34322)
                     /locus_tag="IW662_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ0865681.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34472..35518
                     /locus_tag="IW662_000839"
     CDS             34472..35518
                     /locus_tag="IW662_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0865682.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35520..37673
                     /locus_tag="IW662_000840"
     CDS             35520..37673
                     /locus_tag="IW662_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0865683.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11190 a         7797 c         6736 g        12139 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctcactct taatatatta ggatctggat tgaatcctct taaaatagga
     4681 tttttcactt tgatagtctg catgattcag gctccttttc tcattcttac tcgatataaa
     4741 ctgaatgccc cagctgcgaa ataaaaaaat gcaaaaggaa acgcaataaa ctgagtgcca
     4801 atcaacaata tgaatcctgt tataaaatac aaaccagtgg cagttgttaa gtattttcct
     4861 ttagcagtca aaaacaatgc tactgctaaa aaagcaagtg aaataaacaa cacaacggca
     4921 aacagaccag tcatgtcctg taataatcca aatgtatttt ctaaagacaa agttgaatga
     4981 tctacagcaa gcgactgata acttttttta aactctgacg cagacatatt acttaaagta
     5041 actgctaacc cgccaaagaa aatcaaagat agactgccac cgaccattcc tagcaatagt
     5101 tcccattttc ttttcatttc tgaaccccct gcttttctaa atgtatccat tcaggtcttt
     5161 tgatcgatgt ttcataattg ttattacgtt cagagaattt gacagtctgt tcatttttct
     5221 tgtcatccca attatcccaa ccagttgggt gaacatgccc acctatctta cacgcttcaa
     5281 ataagacatt tgcatacgga cgccacggtc ttcctaaata gacgtttcta gtatcaggtt
     5341 ctgcagtcaa ggtacagctt tcaaaacgat accctgtttt ttgatttttc ggcgtagatg
     5401 ctgctgtgat gtatccagga tttttactgg ctaagctatg gatatgacac tgatgaaaaa
     5461 cggcatctgc cccaccaaat atgaaatcca ccgttccttc aatccagcaa tattcatagg
     5521 tctgacgaca atactcagga ttggcagaag ccggtgtaca aaaaggagta ccatcttttt
     5581 gcaaatcagg caacggaccc gtgcataagg tatcctgata accagatagt cgacagtttt
     5641 ttaaagatac gcgatctcct gaattgaata aagcaacagc ctgtcctacc ttttctcctg
     5701 gacctgctgt attttcgatg tgaagatttt ctaatacgac attcgatcct tctaagaaaa
     5761 aagtagcact tcggaaagtc cctaactggc taccgtcaag atactcctgt tttgcaaaac
     5821 gattaccgga gataactact gttcctgctc ccacaagttt aaaattggat aatctgcttt
     5881 ctatatattc atcatatctt ccttctttta ctatcaagac ctttggatgg ctatccggca
     5941 ggcctgccaa ataatcgatt ccttcttgga tagtttcaaa atccgtcggg gtatctatag
     6001 cattctttcc aattatcagt ttgtctttca tcctcataaa tgctccctta tgaattgttt
     6061 ccaaatattt ctcatttctt gtgtttccat atgaccgcct gttaaagaaa aagaagaaaa
     6121 gttttctgga ctacccattg attgatactt tttgtccagc ccttttctca gcttcagtac
     6181 gccttcgtcc aagcacatcg tatcgttttt tccaactaag ctaagacgag ggcgcggagc
     6241 gattttttct tggattgcta acgttgtata agctgtcaat agatttggca catagtaata
     6301 aaacccatga tgatctaggc ggcgatgtag caaaagtatt tcgatgtcca cctgtgccgc
     6361 tagatcaata caaactttga cacgagaatc caatgctgtc acccaccagc ttaataagcc
     6421 tcccatagaa agaccaatcg ttgcgagccg ttgagtatct gtgtcttccc ttgtctccaa
     6481 ataatcgatc aagctgatca catcataaat acgcattccc cataacgttt ttcctgttaa
     6541 taaaaattgt ttgaacaact cgctttcaga aatacctccg cgttcttcaa atccccaagc
     6601 atcaatcgac caaacagcat atcccattcc tgtcagtact tctgcaaaag atggagaagc
     6661 taagtaagga gcaccatgga tcaattcact tttcccgact gaaaaatctc cgccgtgaga
     6721 atgcaggtaa aggaccggcg gaaacggtcc ttctttttct ggataagcat agagggctgg
     6781 tactgtttcc aattcattta agtacaattg atagtattca agcataaaac cgttttgttt
     6841 ttctcggtat aatcgttttc cactaggggt atgtctgcta gatggagcac ctaaaaaatc
     6901 attcaactgc ataagcaaag tttttacgaa caaccagttc atccatattt tctgattgat
     6961 ttgattggaa tttctcaata ctgatttctt tagtattttc taatagaaat gctggtccct
     7021 cgtgattttc aatcgacatc tggttaaagg aaaccttacc cgcaaagcca atataaaatc
     7081 cttgtctgct catttcctct attcctgtca tcatagcagg aacaccggga actgcatctt
     7141 gggccatcga gacatcgata ttgtcaaatg tgatttcttg tatgaattgt tccgctaatc
     7201 cataaataaa acctgcagaa gcatggacat tccttgccgt gatattcgaa aaatggatcc
     7261 ttctgaatgc tggtgtccgt tcatctatcg gataagcctt tttttcccat acatacggtt
     7321 ctttccctcg cggaccacag aaataataaa gattcagtat aaatggacac atcacattat
     7381 ccatgactat attggatact cgaatatctt caacgatccc gcctcgacct cgtctagatt
     7441 tcaatcgtat tcctcgatca gtttcttgga agatacaatt tgaaatggta atattacgaa
     7501 tactaccact catttcactt cctaatacga caccgccatg tccatggacc atcgtgcaat
     7561 ttgtgatcgt gatattttcg caagcgatcc tttcgtacgt atcttcagtc cctgccttga
     7621 ttgcaataca gtcatctccc acgtcaatat ggcaattact aatacgcaca tttttgcatg
     7681 attctggatc aattccgtct gtatttggtg aatccgctgg gttcaagatc gtcaagttat
     7741 caaacgttgc attactgcaa agaatcgggt tgatcgtcca acttggtgac tggatcaatt
     7801 taatatcttt cacagtaatt cgatgacagt tatggaaact catcaattta ggacgaggat
     7861 aagcgagatt atctggttca tttctaaaag tatgccacca tttttttcca tttccatcta
     7921 atgtaccgaa accagtgaca gagatgttct ctacattttg agcatagata caggaagcat
     7981 aaacttttcg atggactcct tcccaacgtg aatgaacaac aggataatct tttggatcgt
     8041 cagagaattt cagaaccgct cctgcgctta aatgaagttc gacattgctt tttaaaaata
     8101 aagctcctgt tagaaattca cctgctggaa caactacggt cccgcctccg tcacttgctg
     8161 cagcatcaat ggcttgttgt attgcttcag tattcaattc atcaattgat gcgccaaatg
     8221 tcaaaatatc atacatattt ataatgcctc cacttcaatc aatgcttgca agaatgcacc
     8281 aacgcctttt tgatcattac aaataattgg ttcactgatg tagtaagcat atgaaccatc
     8341 tcgctgatct gctccgccta accctgctac ttgacagttt ttattcaagt tcagccagcc
     8401 ttcttttgtt tccagaacaa attcatcaag taatccttga tgtgatttct tcacttgtgg
     8461 aagccattct tctttgttca gcagtcccaa acgtattcct ttagccattg cgtaaacaaa
     8521 catactactg ccggaagctt ccagataatt tcctttccgt tttgtttctg ttgttacttg
     8581 ataccatgtc ccgctgtttt cttcttgata tggaagtaat gcagccagtg tcttttctag
     8641 aatttctttc agcaaagaac aatctgtttc ttcatgaagt aattcatagg tatctgctgc
     8701 agccatcaaa taccagccaa tcgatcggct ccaaaaatga tgggatagac ctgtttttgg
     8761 atcagcccat ggttggacct tcttttcatc ccatgcatga atcagcaaat ttgtttttgg
     8821 atcgaataga tgtttataac tgatttcaaa ctgtctcaaa acatcttggt aatctttttc
     8881 ttcactaaaa ttctggataa attcagcata aaaaggagaa cccatatata agccatccaa
     8941 ccaaatttga taaggataaa tttctttatg ccaaaaagct ccttcggatg tgcgtggatg
     9001 gtttgccaac tgctgggaaa ggagttctgc tgctttttta tatttttctt cgcctgtttc
     9061 tttgtacaat aaaaagaaaa gctttcctgt atttacatgg tcgatattgt attcttcaac
     9121 gctgtagcca cggatcgttc catcttcttg cacgaaatag tcgatattgt cttttatgaa
     9181 atcaaaataa attttttctt gggttcgttc ccaaagtaat tggaaccctt taagtacgac
     9241 gccatagtca taagaccatt ttcctttata ccacttttct tcatataagc gaggtgttct
     9301 ttccatgata ctttttgcta atttctttga ccagttttcc attatttgct ctcctttaaa
     9361 aattaaaata ggatttggga caaataccaa gaagtatttg tcccaatcgt ctatattact
     9421 gtgccgctgc tttgtatgct tcctcgtact cttgtgtgat tcgatcgcct ccttgtgctt
     9481 tccagtcttc tacagctttt ttgaatccat cttcgtcaag ctgtcccatg atgtattgca
     9541 ccgtagcatc aatgacgatt ttttcaattt ctgatcctac ttcattgttc gtcggtgaat
     9601 ccaatggcac agaaggatcc attacagcaa attcttcatt ttctttgatc aattgattag
     9661 agaattcttt ttcagggtct gtatctttga aagagaacgt cacttcacta ggacgagagc
     9721 ttgataacgg ttgcacctct tgttgccaaa gatccatatc gataaattcg atagaaccat
     9781 catcattcaa ttcataatgc gttccttcga taccgccagt catcaattta tacacatctt
     9841 cttctaaaag atcatcaaca aattgaagta atttcttcaa ttgtgcttcg tcttttactt
     9901 gagatttggg aaatgctagc aagccgccaa taccatttcc ttctgaccaa acatgataac
     9961 cgccgccttc accatctgag atcttgttta caggcactaa ttccatatca tcttgaatac
    10021 cttgacccat cgtcttcaga ttcttgatat ccaccattcc agtgtagatt ccagttcttc
    10081 cttgtgcaaa ttgttcttgc tgatcagtct tttgggtaac tgcaaagtct tgtgccaaat
    10141 agccattctc aaatagatct cttgaaaaat tcaaagcttt catatactct tctgtatcaa
    10201 attctggagt gaatttgcca ttatcgtcta cagcccagcc atttggtgct ccaaaataag
    10261 aagtgaaaag tttaaagctg gtataacgaa tatcactagg cgcacgatcg ccaaaaccaa
    10321 ctgtgtcgtc cacaccattt ccatcaggat cgttttctgt aaattgttta gctacttcat
    10381 aaagctcatc catggtagtt ggaacatcta gtcctagatt atccaaccag tcttttcgaa
    10441 tgactaagcc agcacgagcg tagtcttttt gtatcggtac gccgtacaag gcaccgtcga
    10501 tactcgcagc ttcgattcta tcttcagaaa tttgacttaa atttttatag tcttttaaat
    10561 attctgatac atcccagaat acgcctgatt ttaacgcatt gcggacagaa gagtttttca
    10621 acatcgcaat ttgaagtgta acgatatctc ctagttcatc agaagccaat gcagttgtta
    10681 atctttcttc ttttgaagca tcaggaatcc agttgaatgt cagttccgta tcggtttttt
    10741 cttcgatcaa gttcacaatc gtgtctgtag gcggtgaagc atgatgcaaa atatttaacc
    10801 aagtgatttt agcttttcca tcttctgtct ctgaagaact gttacttgaa ctgctgccgc
    10861 atgcggcaag tgctagaatt cctgcaaaac taagacctaa ttttacgagc gttgcttttt
    10921 tcataaattt tctccttcac tgttttaata atatttttgt tttcttctgt tttttctgta
    10981 tatgagttct tccattccaa atggcaaaat atagaatttg ctgtccacgt gactgcggaa
    11041 aagaagagac tgccaccgat aaaggttaaa acacctggca ttgtttggat gatcatcaga
    11101 taaatcccta ccatacttaa tatcaacact aatgagtaat gaagatgtga gaatccgaat
    11161 aaaaatgcca ttttcatttt gaaaaaatat cctttccaat cataatgagc catgattggg
    11221 aaaatataga gtgctgagag cacatatatg aacgcagcaa tatacaaagc gatcctaaga
    11281 taccaattgt gtatgaaaga aagatccaca agtaaaacaa tcccgatggc gccatatatc
    11341 cagctcatca gcaaggtttc acgatagttt tctttaaaat atgtgaagta cgttttcgtc
    11401 aaaggaaacg tttcttttga gcgcagccat tttctaatca ctgcaaccat cgcatacgat
    11461 gcaggaccga aagtaaaaac tcccaaacct gcgatgacta aaccgcccca cagaaaattc
    11521 aaataaacca actgtaaaat acgatttagc cttccgttta ttttatctaa tgactgcagc
    11581 atatctttcc ctctctttct atcttagtaa acgccgtctt ctcctagctt tttggcaatc
    11641 ttattagcta aaattaccat aaacaatcct acgacccctt taaacaatcc cattgctgta
    11701 ctgaaactta actgtccatt cttcaatcca gctgtataaa tataggtgtc gaaaatttct
    11761 gcaacgcttc gattcaatga attcaataac aagtacatat gctcgaaccc taagtctaat
    11821 gtctgaccga tttttagaat cagcaaggta atgattactg gacgaattgc cggcaatgta
    11881 acatgccatg ttttgcgtaa gcgtcctgct ccatccattt ctgcagcttc atacaactgt
    11941 gtatcgactg cagttatcgc tgaaaggtag atgatcgtag accagcccaa ctctttccag
    12001 atcacttgtc cgatatatac tgtgcgtgtc cattcagggg atgttagaaa actgattttt
    12061 tcaaatccca ttgttgcaag gaattcattc aatacccctc cgtcaacatt caaaaataca
    12121 taagtaatcg aaacaatgat tacccaagac atgaagtgag ggatgtaaat gatcgtttga
    12181 atcccgtttt tcaatcgttt gttcgttact tcatttaata gtaaagacaa aatgattggc
    12241 atcggaaaaa acacgaagat atttaatccg aacaagatca acgtatttct taataatgta
    12301 aagaatgtcg gttctgtaaa caaacgaatg aaatgtttga aaccgaccca aggacttccc
    12361 attattccta aaaatggttg ataatcttga aaagcaataa cgagtccccc cataggaaga
    12421 tacctaaaaa tcaagaaata aaggactccc ggcagaatca tcacatataa aaatttattc
    12481 gttttgattc ctgcaattat tttttttcgt cttctgagct tttcttcttt tttcgatagc
    12541 gtcagttgaa atggttcttg tttctttaca ctttccataa gcccctcctt agttttgtga
    12601 atgtttgctg cttatgactt catgatagat aactatctga attacgtata taatcatttt
    12661 taccgtaagt aaaaacgctt acatagcaat gatttttgtg ctatacaaaa cgataaagtg
    12721 tattttttca caaataaact acgcatagca aaccttctat ttttttctat ctgacactta
    12781 gcataggaaa tgattatata cgtaatcttt caccgatgat aaattgaaca tatagaaaca
    12841 gaaacattta atttattttg aggaggagcc tatctatatg aaaaagaaag tctctatagg
    12901 agaaagaagt tttacacttt tcaacaatac gtttctaatt ttgctagctt tgatctgtat
    12961 cgttcctttt ttaaatatca tcgctacatc ttttgcttct acacaagaag tcgtcgcgaa
    13021 aaagtttatc ctttttccta ctaccttttc tttagacgcg tatcgctaca ttctttctac
    13081 gccgaccatt tttagagcac ttgctgtttc gattggtgtt acaggtgtcg gaacgatcgt
    13141 cagtatgtgt gcaacttcac tgatggccta cggtctttcc agaaagtatt tgtttggtcg
    13201 aggtttcgtc aactttcttg ttgtattttc tatgttgttc agcgggggga tgattccaac
    13261 gttcttagtt gttcgttctc tagggctggt caattcttac tgggccatga tcctgcctgt
    13321 ggctgttaat gcaatgaata tgatcattat gagaaacttt ttccaagcct tgccggatag
    13381 tttggaagaa tcagcaaaaa tggatggttg cacagatttt ggtgtatttt tcaaaattat
    13441 gctgccatta gcgttaccgt ctattgctac tatctcgctt ttttatgctg ttacttactg
    13501 gaacacttat atgactgcta ttttgtatat caacgattca tctaaatggc cgatacagat
    13561 tcttttacgc caaatcgtca ttgtatcaag tgggatgcaa gcagaaagta gtgccgttga
    13621 tgtgattcca ccagcacaaa cagttaaaat ggctgttatc gttattgcaa cagtgcctat
    13681 gctgattgtt tatccttttg ttcagaaata ttttgtcaaa ggagcactcg taggttctgt
    13741 caaagggtga ttatgtactg aacgcaaaat ggtagttgtg tcagaagtgg tcagcttcaa
    13801 gaaataagaa ggcatttacg aaaattgctt cttaaatttt tgtgaaattt cggcttattt
    13861 ttcgaagaat ctactcctgg aacaccgtgt atcagaggtt gtgaaagaga cgttcagcct
    13921 tgagcgatga taaaaactct cacaaacatt gcgacgagta acccacagga gcaataagaa
    13981 gcgattttga aaaatagctt cccatatttc acaaaattat gccttattgt cgatagcctg
    14041 tctcttgaac gccatttatt cgtaaaaaag aactgtgaca agacttttgt cacagttctt
    14101 tttgcgtgct tagccttgaa atttaactat ccataaatac aaatactagg gttattctaa
    14161 cttcttttat tgcttgaatc agtccacaag aaaaacagcc tcttgattca caaatatttt
    14221 tttcgatatt ctcccggcgt aatcgattcc ttctttttaa agaaacgaat aaaattttgc
    14281 ggatttcgat attgcaatct ttctgcaatc tcttttacgg agaggttagt ctctttcaac
    14341 catttttttg ctacctctaa acgatgattt tgaacaaaat caccaaattt ttctcctgtt
    14401 tcctttttaa atacattact taaatagtta gggttataat gcaacctatc agcgatcaca
    14461 tctaaagaca gatcacgatc atattctgtg tagacgatat gcataatatt ctccgacaaa
    14521 tttttaaatt catgatcagt gattgcttga gagcattctg tgattggcag aactaattgt
    14581 tctaaaacaa gtttttctag ttctttggga tgataattgt tcagtgcttt tacataaatt
    14641 tgtttcatgt tatcaaagtt ttttgaatct actcccaata actgtcctaa ttgaacgagt
    14701 tcattgatca agcggaccga aactacttcc cgactcaaag gatttttatt ttgtgcaaac
    14761 aatgcatcaa tcaattcatg tactaaattc ttcacttcct gatttccaga acgaatggct
    14821 tcaaacaatc gattttgttc ttctatcgga tatctaataa gtgttttttc atgagcagca
    14881 ggaacgattt cctgataaaa aataattgaa cttggaccag tatttacacg ataatacagg
    14941 gcttcttttg cccgatcaac cgattgcttg ctttcattca gcgtttcgaa ccggttactg
    15001 atgcctatac tgatagttaa attcaagtat tcttttacac gtttttgaat gagtcggcaa
    15061 tactccatga cttttttgct tgcttctgca tcatctttat ccattcgata aatagtggct
    15121 tgcatttctt cattcaaaac gatcggcagc attcgatttt cctttggcac aatttccgtg
    15181 atcatgttgt tgatcgctaa aagtagcaaa tcacgctccc caccgtgact atcttctaac
    15241 agatcgattt gaaccaaagc tgtataatag cagtcatttt gagaactata accaaattgc
    15301 tgcaaccgct gatctagttc tcgcctttct actcgatttc taaataaact aagtacaaat
    15361 agcgtttcca gctgtggctt ttgggtaact aagtgtgcac ttaacgcttc tttttctcct
    15421 atgatccgac tgactgattg agcgaccaaa tctagttctt tcccactgaa tccttttgat
    15481 ttaagattca gacgttcctg tatttgagaa atcggacggg caaatctgtc cgaaaataag
    15541 taggaaagaa gaccaatgag tcctaccagt gttaaagaca ctgcaactag cccgattctc
    15601 agattgcgga tcattgcgcc aattgcacta gagtcaactt ctaatctgta aatccaatta
    15661 ttataatctg atttcagtac tatatacgtt ttatcttttt ctcttatcac tttcagctga
    15721 ttgtaatcta gatccacgtc cgttaataca gttggttgct ctttattttt tactgtctga
    15781 gtagtaaaaa ggttgccaac ttcgttttca atagaaagta tgtcttctcc actttcacca
    15841 acaacttgtt ctattgtatg tttctctatc gtagcaattc ccaatgcata tttttctttt
    15901 tcaaacattg gcaaagaaat caccatttcg attcctttgt ttttcttttg ccaaaataaa
    15961 ttatttttgt tatttatgta cttctgttga tacttttcaa tttcctcttc ggtcagttga
    16021 ctcaacgatc cattgattac tccccattta ccagccaaac tgactaaatc ataattatta
    16081 tcatccataa caatcgtttc tataaaattt agttctttcc tgatttcaga ataagtggag
    16141 taatctgtat atgtcagcgg atgcttgaat atgtccgaga aatttttggt cgaaccatac
    16201 gtagaaaacg aatattcaat cgttctaatt ttccattcaa cgttagtttt aacttgcctg
    16261 agcaaattct ttttgtctgt taaataactc gattcgatcg attcataggt attctggtaa
    16321 atgaataaac taaatcctat gacaagtacg gctcctaata aaaacatcgg taaaaatatt
    16381 ttataaaata ctgtactttt cttcattttc ttcctctcct ccttttaaaa taaaaggctt
    16441 tcatttttct gtaaaattga tactcatcta attaaatgag cattccccgc tttcaagaat
    16501 agcataatta aaaataaaaa tccaagtaat ttttaacgtg tgcaatcttt tcatatcaaa
    16561 gatctattga cttatcgtag ttatcgtaat atgatgaatg cgttaacaaa aagtgaaaaa
    16621 gtaaggaaaa tgatggaatg gtaacgatca aagatgtcgc taaagcagct ggcgtttctc
    16681 atacgacagt ttctcgtgct ttgaataaca gcagtttaat aaaaaatgaa acaaaacaaa
    16741 aaatacgaca actagctaag gaaatgaatt attcgccaaa ctatagtgca aaaggtttag
    16801 tgaatcaaaa aaaatatcta atcggactat ttttctcaag tttgcataaa ggaacttcat
    16861 caaatttcct agcagattcc attgcaggca tccattcagt attagacaca aattacagtt
    16921 tatctgttga aagtatcgat gttgttgatt taacagaaat caacctgcaa cgctacgatg
    16981 gactgatcgt tatgagtcaa agtgatgatg atcagccttt catcgactat ctcaaagaaa
    17041 aagaatttcc tttcgttgtt gtcaatcgtc acatcaatga tccagcggtt atcaacgtca
    17101 ctgccgatga tgcggccggt gttatgcagg cagtagatta cggtattgga tgcggacata
    17161 cgaagatcgc ttatattggc ggaaagaaaa attttcgttc gacaaaagaa cgggaaaaag
    17221 gggtattgaa aagtctaaaa aaagcgagca tccctgtcaa tccagcctat tttttaacag
    17281 gagattacag cttagaaagc ggcctccaaa acatggaata cctattatca ctttctgacc
    17341 tgcctactct ggttttttgc ggcaacgacg acatagcgat tggtgcattg agagctgtac
    17401 atttagctgg cctccgtgtt ccccagaaca tttctctgat cgggttcgat gattctccag
    17461 tcgtctccta tctcaatccg ccgcttacca ccgtacgtaa gcctttgaaa gaaatgtgtc
    17521 aagaaggaac aaagctttta ctagatttga tcgaaggcag gacgatcgaa caatctaaaa
    17581 ttgagctctc caccttgttg atccttcgtg agtcgatcaa ttacttgtaa actacaagta
    17641 attttatata aaaatgttaa cgtgtgcata acacagaaag gaagtttata tgaaaaaact
    17701 gacaaaagaa ttagtgaaaa aaaacgaagc acctgtaaaa gttcttcagt ttggtgaagg
    17761 caattttatg cgtggattta ttgattggca gattcagcag ctgaataacc aacaattgtt
    17821 taaaggtaat gttgcaatca ttcaaccttt agaacaaggt cttggaaata tgatgaacca
    17881 gcaagataat ttatatacgg tcattttaca aggactcagc aatggcgaaa tcgttgatac
    17941 ttccgagatt atcaccgtag tagaacaaat gatcaatcct tatgatgaat gggatgctta
    18001 tctatctcta gcagaaaatg acgacttggc ctatatcgtc tccaatacaa cagaggcagg
    18061 aatccaattc gtggagactg ataatttaac agatactccc ccacagagcc tcccgggcaa
    18121 actgacagcc tttttatacc gacgtttcaa attaaacaaa gctggcttta ccatcattcc
    18181 ttgcgaactg attgacagaa acggcgaacg tctaaaagaa atcattttac gctacgcaga
    18241 aaaatgggat ttagaacctg cttttgttga gtggttagaa aaagaaaatg ttttctgttg
    18301 tagtttagtt gatcgcatcg tcccaggtta tcctcgcgaa gatgcaggtg ttttcaatca
    18361 acggcataac tatgaagatc aattgatggt caaagcagaa ccattcatgc tttgggtgat
    18421 cgaagggcct aaagaattgg agaatcaact accattcaaa caggctggct tgaatgtcat
    18481 catcacagat gatatgactc cttatcgtga acgtaaggtg catttgctta atggaccgca
    18541 tacagcgatg gtccctcttg ctttgttggc ggaattggaa actgtagaag aagtaatgaa
    18601 agatccttta ttctccgcat atgttgatca gttattcaat ctagaattga ttcctatgct
    18661 ttctttacct aaagatgaat tagccattta tgctgaccaa atcaaagaac gtttcttgaa
    18721 tccttttgcc catcacaaat tagaagcgat ttcattaaat agtgtttcaa agttcagcac
    18781 ccgtctgctt ccagtgttca agaagtatat cgaagaacaa aatcaggtac caccgttaat
    18841 cactgtttca ctagctgcgc tacttcttat gtatcgaggc gatcaagtaa agccacatga
    18901 tgatgaaaag acgataagcg aatttactga tgcttggtct gataatggaa cagctattcc
    18961 tcgattattg caaaacgctg cgctttgggg agaagatttg tctcaaatac cgaatgtgac
    19021 agatacagtt caagaaatag ctgcaaagat cgatcaaaaa ggtgtccggt cagtattaca
    19081 agaaatgaaa ggaacgatgg tccatgcttg aaaccctgca accaacaatg aaattaaatc
    19141 gacgagacag tgtggtcgtc gcattaaccc ctattccaaa acaaacacaa ctgacgattg
    19201 atcagcaaac gatcaccact cttgaagata tcccgcaagg tcataagatt gcattgggtg
    19261 atttgaaagc tggagacaac gtgatcaaat atggctatcc aatcggtcat gtgacgacag
    19321 acgtaactgc cggtcaatgg ctgcacactc ataatgtaaa aaccaatctt tctggtgaat
    19381 tggattatcg atatgaaaaa gatgtccatc caagtcatta cctttttgag aaccgaacat
    19441 ttcaaggtta cttgcgaaaa aatggaaaag tagggattcg taatgatttg tttttagtac
    19501 ctaccgttgg gtgtgtgaat ggcattgctg aactgatcgt caaacagttc aaggaaaaac
    19561 atccagatct gggacagttt gaccatatta cgattttaaa gcatccttat ggctgttcgc
    19621 agcttggaaa agatcatcaa aatactcgag aaattttagc ggacgcagta aatcatccca
    19681 atgccggagg agttttagta tttggattag gttgtgagaa caatacagtt cctgaattca
    19741 aaaaaatttt aggtgaatat gatgaagaac gagtgaaatt tctagtggct caagatgttt
    19801 acgatgagat cgaacaaggt gtggctctat tagaagagct tttaacagcg gcggaaaatg
    19861 accagcgcat atcagtcccg ctctctaagt tgaatgtcgg tttgaaatgc ggcggttctg
    19921 atggtctatc gggaataact gctaatcctt tacttggtgc attttctgac tacttgattg
    19981 ctcaaggagg cagcacgatc ttaacagaag tgccagaaat gtttggcgca gaacaagtat
    20041 tgatggcacg agcggaaaat gaagaagttt ttgactcaat cgttgacttg atcaatgact
    20101 tcaaacagta tttcttgtct tacggggaac cagtttacga aaacccatct cctggtaata
    20161 aagcaggtgg gatcactaca ttagaagata agtctttagg ctgcacgcaa aaatcaggaa
    20221 gctctcctgt cgttgatgtg ctacaatatg gcgaaaaaat cagaaaacca gggcttagtt
    20281 tattgcaagc tcccgggaat gatcttgtag ctgcctctgc tcttgcttca tccgattgcc
    20341 agttggtctt gtttaccaca ggaagaggga ctccgtttgg ctcatatgtc ccaacggtca
    20401 aggtatctac gaatacaact ctttttgaac gcaaaggtca ttggatggat tttaatgcag
    20461 gcgtcttatt agaacaacca atggaagtaa tcttagaatc gtttgttcaa aaaatcatcg
    20521 ctgtagctag tggagaagaa acgaaaaatg aacaaaatga cgttcgagaa attgccattt
    20581 ttaaaaacgg agtaacactt tgattttcat aaaacagtat aaaaagaaag aaggaagatt
    20641 tatgttttta tctgatgatt ttttattaaa agacgattgg gcgaaaaagc tttaccattc
    20701 ctacgcaaaa aacatgccca ttattgatta tcactgtcac ttagatccaa aagaaattta
    20761 cgaaaacaac aatttcagca atctgacaga agcttggtta ggaggcgatc attacaaatg
    20821 gcgcttgatg cgagcttgcg gcgtgcctga agaaaaaatc acaggaaatg cttctgattt
    20881 tgaaaaattt ttagcttggt gtcaaaccgt accaaaaatt atcggaaatc cgctttacag
    20941 ttggtcacat cttgaattga agcgtttctt cggtattgac ttgttgataa atgaagaaaa
    21001 tgcagagaaa atttggacac aagcaaatga aattttggca gctcctgatt ttcgccgtcg
    21061 ggaaatcgca aaaaaatcga atgtagaagt gatttgtaca acagatgatc ccgcagacga
    21121 cttgcactat catcagttgc ttgctcatga agaaccagat ttgaaggttc taccttcatt
    21181 ccgtccagat aaagcactca atattgaaaa agaaggattt aatgcatggg tcacactact
    21241 ggaagaagca gctgaccaaa cgatcaccac ctatcaagaa ctgatagaag tcttagggca
    21301 gcggatagaa tacttccatc aacatggatg tcgtgtttcc gaccacgctt tagacatctt
    21361 acgctataaa gaagcggatg aaacagtatt agaagagatt tttcaaaaag cacgagctaa
    21421 caaactatta tcttcagatg aaatcgatgc ttatcgtaca gaaacactga tacatttgac
    21481 ccacttctat cattctcata attggacgat gcagctgcac atccatgctt atcgtaactg
    21541 taatacacaa atgttggaaa aattagggcc cgataccggc tatgatggaa tgaatgatct
    21601 ttctttgact attcctctgc aaaaattatt gaaccgtgca gaagaaacgg atcagttgcc
    21661 aaagacgatc ctttattcat tgaatccgaa tgactatcct gcgattttag ctttgatggg
    21721 ctgtttccaa aaagaacaga atggaaaact gcagttaggt tctggatggt ggtacaacga
    21781 tactcgagct ggtatgcgcg atcaattgac acaatttgct gatggtagtg ctttggggaa
    21841 ttttgtcggc atgctgacag attcaagaag tttcctgtcc tacactagac atgaatattt
    21901 ccgtcgtgtt ctttgtgaat ttttaggcga aatggtcatg cgaggagaag cacctgaaga
    21961 tgaacaactt ttaggaagtc tagttcaagc gattagttat acaaatgcta aaaattactt
    22021 tggctttttt gcctaattga ttaatagtaa cagtaacgaa tcaaaaacag gatagtgaaa
    22081 taacggaatc cgtttatcca cgttttagag aagaagacgg accaaatttg ctccgtcttc
    22141 ttctcttgct gttctgcttc attgcctata aacctttatg aaagaagaga taacatgcct
    22201 actatccata ttgccggtga ttctactgcg gcagaaaaag atgaaaacaa gcgtcccgag
    22261 acaggttggg gagaaaaaat ctctgcttat ttttcacccg agatccagat caataaccaa
    22321 gcaaagaatg gacgcagcac caaatcattt atccaggaag gaaggttagc ccgtctagct
    22381 tcacagtttc agccgaatga tttcttattt gtacaatttg gacataacga ccagaaaatc
    22441 caagaagaaa gaggtacgct tcccttcggt agctatttaa aaaatctgca gatatttttt
    22501 gaatgcgcaa aaaaagcgaa cgtccaaatg gtcttactga catctgtgac gcgacgagat
    22561 tacttagaaa acggaacatt gaatccagat atacttggag actatccaaa agcgatgcgt
    22621 gcatttgctg agaaacacca tattcctctt ttagatgtgt tctcaagatc acaagaattg
    22681 ttccagacct tctcaaaaga agagacaaaa aaattctacc tgcatcttat acctgatact
    22741 tgtaaaaatt acccagaagg actaaaagac aatacccact ttagtccaga aggcgctgat
    22801 aaagttgccc gaataattgt tgaattgata aaagaatccc gtctgcctct tgctaattat
    22861 ttacaaaaag gagtttgaga tgtttccatt aaatttaggc atccgtgccc acgatttaga
    22921 tgttcgaaat cgtgaagaaa tcgttcaaaa agtatcacag cttcacttgt cccatatcca
    22981 atttgcacca caaaaagcat ttcctgaaca acttaaatta actgacatga cacttgggag
    23041 tgctgcctat tttggtgaat acttccaaaa aaacaatatc gaactttcca ttttaggatg
    23101 ctatatcaac ctatccagca aaaatcaaga agttcgtgca caagcattgg caactttcaa
    23161 acagcaaatt gctctgtgct ctgcctatca agccaagatg attgctacgg aaaccggtag
    23221 tgtctctgtc ggttacacaa aagaaaattt tactgaagaa gcttatcaga tcgccagaaa
    23281 ttctatcatt gaattagtcg ctcatgcaga aaattttggc gtaactgttg ctgttgaagc
    23341 agggatcaat catccaatct ataactatca gttagctaaa cgtttaatcg atgaagtagc
    23401 ttcaccaaat ttaaaaatca ttttagattg tgcgaatttg atgcataaag aaaatcatgc
    23461 tgatcaggaa aaagtgatcc gaggtgcctt ggacaattta catggttata ttacagcgct
    23521 tcatctcaaa gactatatca tggaaaatgg aaagatccgc atggtacctg ttggcaaggg
    23581 ctgtttagat ttccgcccgg tactgcatta tatcaaatat gagcgtcctc taatgtacgc
    23641 cactcttgaa gctactcctg agatttatgt tcctgaagca atagaacact tgcagtttat
    23701 ttaccaagat tgttaaggag aagattatgg aggaacgaat acagaaaagt tatcaaaaat
    23761 atcatgcaaa tgcttttcag acattgctgc atctttatca gggaaattat tggcgttttg
    23821 ccatgtcagg actctttttt attatcaaac atcttccatc ttggttaatg ccgatcgcta
    23881 ttgccaatgt aatcaatgca gcttcatcga aagattcttc tcagttgcag gccatttatt
    23941 tcaacgcttt tttgatggct tgtctgattg gtcaaaatat attgacgaat tattttcatg
    24001 taaaatacca tagcacttcg atccgtcaag tagaagctgg actaagagcg acccttatca
    24061 agaaaatcca agaactatct attccttatc aaaaagaatt gcaatctgga cgaatccagt
    24121 caaagatcat ccgtgatgta gaagctatcc aaacgttgtc ctctcaagta tttgtcagcg
    24181 gattaaatat cgctgtcaat ttattcgtag ctttaggtat cacgctttat aaaagtcctg
    24241 ttgttttcat gtttttctta ttgactgttc ctctcgccgc attcgttact ctctttttcc
    24301 gtaaaaggat cagacagtcc aactcagact tcaggcaaga aatggaagag acttctgcaa
    24361 aagtgatcga agtgatcgaa ttagcaccga ttgctcgagc tcatgggtta gaaaaacacg
    24421 aaacaactcg cttaaataag cattttactc aaatctatga aaaaggattc cacttagata
    24481 tcctgcaatc tgttttcgga tcagtcagct gggctagttt tcagatattt cagctgattt
    24541 gtttgatttt cactgggata ttagctttac aagggcgcat ccaacccggc gatgtcgtga
    24601 tgtatcagac gtatttcacg acgatcgtca attccgtttc tggaatgatc acgctgattc
    24661 ctacgatttc aaaaggcatg gaatctatca cttctattgg cgaactgttg attgctggcg
    24721 atgtagaaac ttatcacggt aagaaaaaaa tccaacattt ggatggaaat tttcgttttg
    24781 aggatgtttc ttttctttat ccggactcac tcaaaccgat tatcgatcat ttttctctag
    24841 aggtgaaagc tggagaaaca attgcttttg ttgggccatc tggctctgga aaatctacac
    24901 tccttaactt attgattgga tttatccagc cgacttcagg aaaaattttt ctagatgatc
    24961 agcctttgga tgaactggat atgcgaagtg tgcgaaatta tttagccgtt gttcctcaaa
    25021 ccactttgtt gttctccgct tcgatcaaag agaacatcac ttatggcttg aaaaacgtat
    25081 caaaagaacg tttagatgaa gtcatcgaag ctgcgcaact ttcttcactg attgaccagc
    25141 ttcctgatgg tctggatacc ttagtaggcg aacatggcaa caagctgtct ggtggccaaa
    25201 agcaacgtat ctccattgca cgagcgttga tacgtcagcc aaaaatcatt ttgctagatg
    25261 aagcaacctc agctttagat aaccagtcag agaaaaaaat ccagcaagcc ctgaattacc
    25321 tgacagaaac acccactact tttattgtgg ctcatcggct ttcaacgatc aaagaggcag
    25381 ataaaatcgt tgtgatcgat gaaggaaaaa tcgttgaaat aggtacttac gaaaaactgt
    25441 tagctcaaaa aggctatttc taccgcttga aaaatgaatg acaaaaaata ggagctatga
    25501 caagactttt gtcgtagctc ctatttctga ataaacggtg ttctcctgcc aactcctcgg
    25561 tcacaagtca caatagtcat caaacatgag aagctgtttt ttgaaattgt tccttgtaac
    25621 tcagagttaa acggtaggct cacgacctct tggtaaatgg tgttcaagag acagcctatc
    25681 ggcaataaga agcaattttg tcatcgctca aggccgaacg tctctttcac accctctgtt
    25741 gtttcataaa atagttttat tcgctgtaaa ggtcgcgaac tggtgtcatg atgatacggt
    25801 tcaagtcatt gatgattgta ctgaagcctt gctctttttg catcaagtca ttgattactt
    25861 cttctgattg gattttcaca gccatttctt gtgctttcat tgcatcttcg tcgctgaact
    25921 cttgtccact catttgtttt tcttgtaatt cgatttgcat cgcttggaag tctttgaaca
    25981 gtttgtgtgc ttcttcgttt gcttgtactt tctcgtacgc ttctttcaac gctttgaatt
    26041 ctggtaattc acggatctca cgttcgattt gattcgcgct atcgtagatg tttgacatgt
    26101 tatgccctct tttcttttag tagttactct ctattgtaac atggggttat tcgttttcaa
    26161 aggggctact ctccttgata tttccgccag aggtctttag ccgcttcacc aattccctga
    26221 acgccttctt tcaccatatc gccgaagttt ttcagccctt cttttgcttg ttcaccatat
    26281 tgattcaagt tatcttgcca atttccagac tctccattag aatttcctgt gtttccaact
    26341 tgatctgcag ctaccacttg tcctcctgtg gcataggcat ctgctacact aaatcctgtt
    26401 tttgaagtat acggaaggat gccttctgcc gctgatttga agactttccc gacaacattt
    26461 ccacttgtcc cttccaaata gtgcagcttg ctggattctt cgtaacccat ccatgtcgaa
    26521 atcactacgt ctggcgtgta accgatcatc cattggtcat ttgcttttgt ggcatcgaaa
    26581 ttagtttctg tggttcctgt tttccctgct atcgtataac ctgcaggatt cgcagcagcc
    26641 gctgttccat ttgaaaatgt accaagaagc atgctggtca tttcatcggc tgtttccttt
    26701 gatatgacct gttttggttt tttgtttgtg ttatcgacga tcaccgctcc agtcgaatca
    26761 acgattttcg tgataagatg cggcgtatac atctgaccat cattggcaaa tgcactgtaa
    26821 gcacccgcca tgatcaatgg agatactcct ttttccagtc caccaagggc taagccataa
    26881 tacttatcag attttgctaa aggcaggccg aattcttctg ctttatcata tcctttttgt
    26941 aaaccgatct catgcaaaag ccatacagta ggcaaattca aactttgtgc aacagcttga
    27001 tacatcggta cttctccttg gtatgtcccg tcaaaattat gcgcatcgta ataactttgc
    27061 ggttcgtctt tcaagatact ggaaggcttg tatccagctt ccagtgctgg tgtatatacg
    27121 cttaatggtt tgatcgttga tcctggtgaa cgacgcatct gtgtagcaaa gctgaatcct
    27181 ctaaagacat gatctcctct tcgtcctact agtgcttgta caccacctgt attcggatct
    27241 aaagctactg aagcagattg gaccatggca ccatctgaag catttgctgg gaataatgca
    27301 ctgttttgat aagtagcttc taattgcttt tgataatcct gatctaaagc tgtatagatt
    27361 ttatagcctt tattcatgac atcttcttca tcaagaccat actcgttaac tgcttcatcg
    27421 atcactgcat caaaataata aggataacga taaccagaat ctgaattatc gtatgtgtca
    27481 ttaagatacg cgctgatatc tgtctggctt tctgcttgtt cttgttcttt tgtgattttt
    27541 ccgttttctt ccattacact tagtactgtg tttttccgat cattcgcatt ttttgggtga
    27601 tcgatcggat tataaatacc aggacctttc agcattcctg ccaaagtagc agcctcagac
    27661 agcgtaacat tcgttgcatc tactccaaaa tactttctgg aagcgtcttg gacaccccat
    27721 accccgttgc caaaataggc attgttaaga tacatggtca agatttcttc tttgctgtac
    27781 ttcttttcga tctcgattgc caaaaataat tccttcgctt ttcgatcaaa cgtttggtct
    27841 aatgtcagat acgcattttt ggcaagctgc tgagtgatcg tacttcctcc gccccctgat
    27901 gttccgccag acaagaccat tcggactgct gcacgggcaa tccctttgat atcaaacccg
    27961 tgatgctcgt aaaaattcct atcttctgtc gagattactg catcttggat atagggagaa
    28021 atcgcgttta attctacata tgttcctttt tgggcgtaca gtgtgccagc ttcttcgttt
    28081 ttcttatcat aaatgactgt cgattggctt aatcctgatt ttaacgtttc gacatttgct
    28141 tgttttgcta aaataaagag gtagatactt gtcactaaga cacagatcaa accaatcagc
    28201 aaaatcaatt tgttcacatg atattttttc cagatgcgct ttcgccatcg atggaattgt
    28261 ccgagatatg gtctgatcca acgccaaaaa ctgatcaaag cagcttttat cttttctaaa
    28321 atgctttgaa aatccatttg cttcttctcc tgtctatttg attcaatcgt cttattttac
    28381 catagtgaaa gagtgttttc ttcctgctta agttgtagtt caatatgttt ttttacgtta
    28441 gtgcaagtag agtaacataa gaatgaacag aaaaatgtaa gttttttctt aaatatgttt
    28501 taaaaatggt acaattagtc tgcgattgca ctgaaaggag tacacttgtg aaaatcacga
    28561 tcactttacc tatgaaccat cctgaaacaa caattcgcga cttgcttgaa aaggaatggt
    28621 tagttcctag aaaggttcga cactttttgc gtatacgtaa aaacgtttgg atcaatcaac
    28681 aacctgcttt gttccatcat gaagtaaagg gcaatgacca gatcacattg ttattcgaag
    28741 aaacagatta taattatcaa gaagttcaat taggagatgc gaagcacatc cgtgttttat
    28801 atgaagatga gcatttgatt atagtggata agcctgcagg aatgaagact catccaaatg
    28861 aaccgacaga aaacagtacc ttactcaatc atttagctgc ttatctcgac actaaacagc
    28921 aacgacctta cgttgtccac cgcttggaca aagaaacaag cggtgccgtt ttatttgcaa
    28981 aaaatccatt tgtcttgccg atacttggaa gaatgttgga acaaaaaatg atctatcgga
    29041 gatatcaagc catcgtatgg ggagaaattg ccaaagatat gaccatcacc aataaaattg
    29101 gacgagatcg ccatgatcgg agaaaaaggg tcgtagatga taaaaatggt cagagcgcct
    29161 tgacacatgt cactgtcaac aaagtacttt ctgaacaaac acaagtctat tgtgttttgg
    29221 aaaccggacg tacacaccag atcagagttc atttagctca taagggacat ccgatcgttg
    29281 gagaccctct ctaccaaaat agaacagctg aacgactgat gctccatgca ttagaactgc
    29341 acatgatcca tccatttact caagaaaaaa tagtcggtaa agctttacct ggattatggg
    29401 aatagaaata aaaaagaaca gagtcttatc agcagataat gataagactc tgttcttttt
    29461 gtgtaatttg tctgcttact tttgatgttc gtgttctgct aacagcaaac taccgataat
    29521 cccactttca ttcggcagtc cccaacgaac gatatattca cttacttgag gtgtttccat
    29581 ataaccaccc atcaattcaa caaattgctg acggattttt tgaaggagat gatcctgatt
    29641 cattacccca ccgcctaaaa taatcttttc aggtgctaac gtcaacgtat agttgatcag
    29701 tgcttgagca atataataag cctgaatagc ccaaacagga tgatcttgcg gcagatcttg
    29761 ccctttgatc cctgtgcgtg cctcaataga cggtcctgct gctaatcctt ctaaacaatc
    29821 tttatgataa ggacaagtac cttcataatg gtcatctgga tggcgtttca cccaaatatg
    29881 tcccatttca ggatgtgcga tccctgaaaa aatttcttca ttgatcacga cacctgcacc
    29941 gattcctgtt cccacggtaa ggtaaataca actatttttt ccttgtgctg ctccttgctt
    30001 caattcccca tatgcagcag cattgacatc cgttgtccaa gcagcaggaa tattgtagcg
    30061 ttccttgatt gtgccaagaa aatcgaattg tgcccaccct tttttgggag ttgccaaaac
    30121 gtatccgtaa ttatcttttg ttttgtcgat accaatcggg ccgaatgagc caatcccgat
    30181 tcccttcaat tcataacgat caaaaaattc aaatacctga tccaatgttt cttctggtgt
    30241 tgtggtaggg atacttaatt tttctaaaat ttctccacca tctgaaaccg cacacacaaa
    30301 ttttgttccg cctgcttcta ttccgccata cattcgtttt cctcctcgaa tccgattgtc
    30361 cgaatctcat aaggttggat tattgggaca accgcttgct tcgttggttc ttcaagcaga
    30421 ttcaatatga cagtttttcc attgtcttta gttatttcca atttttctaa atgactagac
    30481 atattgaaac ctcttactac tactttatca ctaaacttgc tgcgtttcaa agctgtaatt
    30541 gcaaacgttt cactttttat cgttaaatat tgttgttttg aaatcaatgt tccttcatga
    30601 tgtttgattt gttgagtgga aaaaggaatc tgtgctgcgt atgcatgcaa ataagtagag
    30661 aatttttctt ctggtccatg tagttcaatg ctgtaattga accgatgctc acctagacac
    30721 tgtgcttctg gtgttggaaa atatccccaa tcgcctagtt ctcccacact gcgaagtagc
    30781 gtcactgcga tttgtccgtc ttctgttact tcatattcat tcaaaccaaa gtttccgact
    30841 gttacaccgt actcttcatc atgaatattg acaaatgctt gctgatgttg cggattcgtg
    30901 gggttttccc aacttttaga cacatggttt gggcgcttca ccacttcaaa aatactatct
    30961 gcttcgtgat gttccacatg taactttgta ggaaacaaga cacgtaagcg atgatctttc
    31021 atctgattgt cgagagtcgt ttcaaagtcg atttttttac tatcttttct gattgtcatg
    31081 attgttttta tttgaaggat tcgtttttct ttggatcgtt ctgcttttcg ataacgaaat
    31141 tccacaacca tctgctgttc tttttctaaa cgctcgtctg cagaaacagg gatttccatt
    31201 tcctgtagaa tacttatttt tgctatttcc ggtgtatcta ctaatacttt tacttcagag
    31261 ttttctttgt tactggatag gattggctga tcacagaaag gttgtttaaa gatatactca
    31321 tttcctatat cgccgatatc ttcaaaaata tgcagattat tcaactttcg tttgtttttg
    31381 cgatcttcca ttgtaatggt accatttttt tcaatcgtca aatgtagagg gccgttttcg
    31441 atgattcgtc cactctgatg aatcattgtt tccttttctt ctgctttcgc ttccccttct
    31501 acaagcgcga atgtttccca agaaaatgct ggcatgtttt ctaatggtaa agtcactttg
    31561 atcattcttg ccatatatgg cacacggaaa cggtctttcg gcaaatcata accaaattgg
    31621 acagtttctt cagataaaat agctggtata gtggccccac tttttgtttc tacatgatac
    31681 ttacgtttag gcaaattttc taactcttga tatagttgtt caggtattcc ttcagcgaat
    31741 ctttttcttt ctaattcgat ggttatttct gcttcaccag ttttatccat tcctgcagta
    31801 tttacaatga caaatggaaa gctgtctttt gggaatccgg tcgtatcgat tgctgcggtc
    31861 agctgttcta aggaatcttg tgcaagatac tttccaactt catctgcttt ttcaaaacga
    31921 ggtatcattt cccgatggac agaatcgact gaacaaccgc aaatactgtc gtgaggatga
    31981 ttttgcatta atgtcttcca agcataatct aattgatcat ggggatagtt tccactaact
    32041 tcataagcca tcgtcgcaag tggttctgta atattttcta gctgtcgctg gaccttggta
    32101 ttccattgtt ttaagtaaac ccgagcagat gctgtattgg ctaaagtata ccagccatcc
    32161 gtttcttgac ttgtcaattc tccttctact gagcctaagt cttctggcac atctttttga
    32221 actgcttcca gatagtcagt aaaattagaa tgaataaact cgtaatctgg aaacagttca
    32281 tttgcgagat ggattgcttt tgatatatct ttttgtaccg gttgatgatc aacaccattc
    32341 atcatcaata agtggttcgt agaggcatat tgttctgcat ccgctaattt ttgcttccaa
    32401 aaggcaagag ctgcttcttt ttctactgga atctcattcc cgttgctata ccaattggca
    32461 aatagcagac caaagattgc tgtttgatca ggtcctttcc accacatctc tgagtattgg
    32521 gaagaatagt tttctgcttc caaaacttga ttatcaaagc caattggttt tacaccacgg
    32581 ccaaacgctg ctgctgaaat accagcttgt ttcattaatt gaggcgtctg tcccatattt
    32641 ccaaatgtat cgggaaaata gcctaacatg acaggggttc cccatttacg gctttcttcc
    32701 atgccaatca gcatatttct cacgttcgat tccgagctga taagaaaatc atcttgcaag
    32761 atataaaatg gtccaattct caattttcct tcattgattg cttgttggat tgcctgtctt
    32821 ttttctggcc ttacttgaag atagtcatct aaaataatcg tttgcccatc caaatgaaaa
    32881 ctgttgaagg acggatcaac ttcaaatagc tctagtagat cgtctatcaa ttcaatcagt
    32941 cgcatgtgat gctgttcgta agccatgtac cattcacgat cccagtggct atgagaaatg
    33001 atatatactt tctttttcat ttacgatacc tcctactttt ctactctaat atcaaagtaa
    33061 tccatgacta attcgcaaaa catcatattt gcccaagaaa accattctct cgtataattt
    33121 tcaggattgt tcacatcgaa tccttcgtgc ataaggtgtg ttcctgcgtc agttgctaca
    33181 agtaaattca atatccgctc tttttcagat ttgtctgacg ttgtcattcc ttccatagct
    33241 aatgcaatcg gccaaacgta tttttctggc gtatgggagg atccaattcc ttttgcgtac
    33301 tttccttcat agaaatatgg attttcttta ctcaacaatg ttcttcttgt ttgcagataa
    33361 tcttcctctt gttcagaaca atatcccaag taaggtgcgg cgatcagatt gggcacatta
    33421 ctgtcatcca tgactgtggc gtttcccatt ccatctactt catatgcata aatcgtttgc
    33481 cctttttgat taaccgttcg tccgtgattc tgaatcccct cttggatctc tttcttcaat
    33541 ttctgtgttt gctgtacaat ttctttatca tcgagtatcg tttgaaaaat ttcttccaca
    33601 taacctaaca cgaccaccgc aaacatattg gatggtacca gatatccata acggcaagta
    33661 tcatcgctag gacggaaacc agaccatgtc attcctgtaa aagctgtttt tgcacctcgc
    33721 ccgtcattga tcagcgtatc ttcttttctt gtcgtgtctc gtacaaatgt ataaggagag
    33781 tgttcatgat cttgctctgt tttgaacacg tccaagattt ttttgatacc ttcaataaaa
    33841 gattgattga attgatcagt ttttcctgta tttttgtaaa gaagataggc caactgtatt
    33901 gggtagcata aagaatcaat ttcatatttt ctttcccaga tccaatcgtt catttttgta
    33961 tgatctgttt ggtggccagc accgttagct tcttcgttga aggcattcgc atatggatcg
    34021 atattgatat aaaaaaattg ctttttgact aacccgctga tcatgtcagc taattcctca
    34081 tcttcttttg caatcactaa ataaggacga acttgtgcag tagagtctct taaccacatc
    34141 gctggaatgt ctcccgtcaa taaaaaggtg gttccgtcat tttgcttttt gactgttgtc
    34201 aacagagtat tggcaaatgc tgctgtaaaa ttctttgccc agtccgcatg gatatcccca
    34261 catttttgtg tgatatcttc cataaatttc tgcaccgatt ttggtatatc tttataagcc
    34321 actaataaaa ccttctttct atttgatata tctttaaaga ttatacttca tacagccaat
    34381 tatgtctacg ctttcaaaaa atatcttttt atttactttt tcatccatta tggtattatt
    34441 tgaatatgat atatcattaa gaggtgttta tatgaatcaa ccattgtacc agacaatatt
    34501 taatgatttg caaacggcta ttttaactgg tgaattacct gtagacagcc aactaccaac
    34561 tgaaaaagaa ctttcacgta cctataaagt aagcagaatc acttccaaac gtgcattgac
    34621 tgaattagag caattaggac ttatttatcg cgttcgcggg aaaggcagtt tcgttaaatc
    34681 cgcttctccg gaacagattc ttgcttctcc tacaaaaaca cgacggattc tgtttcttct
    34741 tccatatgtc gctgatttgt ctgttggtga ttttagcaag ggattgaacc ctgtgatgca
    34801 agataaacag attgatgtca tgatgacgac gcttgacttt ctacaaaata aaacagcaaa
    34861 agagctaatc aatgaatttg acggattgat ttattatgct cttgatacag aacaacatct
    34921 tgacttactt tttgagcttt cattgaaaga attccctgtc gtcatattag ataaaaaaat
    34981 ctatgagtta ccttttccta ccgttctttc cgataatttc caaggcggtg cgcttgctgc
    35041 ctcttatttg atcgaacagg gacattcaaa aatcgcttat ttgtttggta cgcagactca
    35101 tccacaatcc gttcgacagc gttaccttgg ttaccttgaa gcattggaca aagctggttt
    35161 gactttccac acttcactgg atgacaaaag cgcgacatta gatgaattgc tttcttatat
    35221 cactaagtat gaagtaactg cttttgtttg tgaaaacgat gttgtcgcta tccagaccat
    35281 gaatctcttg agacagcatg gttatcatat ccctgaagat ttttcaatta tcggatttga
    35341 tgatatccaa gcagcagctc ttgttgaccc gccactgaca accatcgcac aagattttga
    35401 acagttaggt aagcttgcgg gagatgcttt ggtcacttgg ttagagacaa aagatcgacc
    35461 atccgatagt aaatttccag tttcattcat caaaagacaa tcaacaaagg agaaataaaa
    35521 tgaatatcgg tcaaatagat actagacatg caacagcgaa tcagccaaat ttctctaatg
    35581 ggaactgcca gccttatact ggcgttcctt ttgggatgaa ttattttgca ccgcaaacta
    35641 cggatcaaaa cggcagctgg tggtttcatc ctgatgaccg cgtttttcaa ggctaccgtc
    35701 ttacccatca accaagtcct tggatgggtg atttcagtca tatgctgttt acgccgatca
    35761 atggaaaact acaggagaac acccttttcc atgctcaaag ttcttaccgc ccagaagaaa
    35821 gtatattttg cccgactcat ctgtcgatct gtcaattacg ttatggcatc tgttctacat
    35881 tgatacctag catgtatggc ggaatcttat ctattgatta cagcaaaaat gacagcggtt
    35941 tacttttatc ctttccggga cgccatcagc tctttgttat agatccttac acagtggaag
    36001 gaaaaatcac acattttgct ggttcagaag atcctgattt ttctttttat tttatattga
    36061 gatttgaata tcctttaaca attgatgagt tagctgtttc caacaaaaat agcgactcga
    36121 tcccgttgta ttttgaacag acaaaaaaac aaactatccg atttgggaca tcttttatca
    36181 gctcggaaca agcagcttat aacctggaac tagaacaaaa ccaaagcaaa gagacttacc
    36241 ttgctcacag tcaagaacaa tggcagtcct attttgatcg tatacagctc acacaccatg
    36301 atcaacaaca attgcggacg ttttatcata atttatatcg ggtgttttta tttccacaaa
    36361 cattttatga aattgatcct agtgggcaaa aaatccacta tgatacgact agtcgatcta
    36421 taaaacctgg tgttctctat acaaataatg ggttttggga tacgtataaa acggtttatc
    36481 ctctttattc tttgattgct gtagaaaaat acgaagaaat gctggaaggt tttttgaaca
    36541 gctatagaga aagtggctat ttgccaaaat ggctgtctcc tgatgaacgt ggacttatgc
    36601 ctgggacatt gatcgatgcg gtcattgcag atgctgctgt aaaagatatc cgacgagact
    36661 tgatgcctga atttctaacg gcaatggaaa aaggtgctac ggttcaaagc aaacatccaa
    36721 attatggacg ccaaggaaca gaagattact tgacatatgg ctatgttcct aatcactacc
    36781 acgaatcagt caatcacaca ttagactatg cttatagcga tttttgtatt agccaagttg
    36841 ccaaggtact agaagaagag gaaatgacga agaaatatca gcagcaatca ttgaactatc
    36901 aaaatatctt tgactatgag tcaggattta tgcgttcaaa agataaagat ggcaagttca
    36961 gagaaccatt ttcagctaat cgttggggac aagattatgc agaaggaagt gcttggcaaa
    37021 gcagctttgc tgtttatcac gatttttcag gactgatcgg aaaatatggt ggcagtgaga
    37081 gatataaaga aaaactcatt gagctgtgca atcagccccc aacgtttgac ccaggaggat
    37141 atggttttga gatccacgaa atgagtgaga tggccgcgat agaatttgga caattagcta
    37201 tttcaaacca gccaagtttt cactatcctt acttgttcag ttatatagga caacctgaaa
    37261 tggcacagcc actgcttaaa cagttgatga ctcagacctt cgacgattct ccaatgggtt
    37321 accctggaga tgaggacaat ggaagtatgg ccgcttggta tatcttcaat agtctgggat
    37381 tttatcctgt cacgcctgga accggtgaat atgttatcgg tatgccatta attgacaagg
    37441 ctgttttgct tttgtcaaat ggaaaggaat tgacgattga agcaacgcct aatcggccgc
    37501 agcaacaatt tatccataaa atcaaacgga tggacaaaga atatacgaaa ttatatttta
    37561 cccatcaaga tttgctgaaa ggcggcacga tctcttacca attagggatc atccctcacc
    37621 ctaagaaata tcaagcaaaa gatttgcctt tttccttatc aaactataat taacaagagg
    37681 ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aagcaaaaat agatcctcat
    37741 atttttcgtt tggttggtct tattaccgca gatgcaagct tttgaacacc gtttattgag
    37801 aggttgtgaa atcagcgttc tgacctgagt attagagaga caaatggcaa aatagttcct
    37861 ca
//
LOCUS       DACEKH010000018        37289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEKH010000018 DACEKH010000000
VERSION     DACEKH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.018"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            108..971
                     /locus_tag="IW662_000841"
     CDS             108..971
                     /locus_tag="IW662_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0865684.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1100..2722)
                     /locus_tag="IW662_000842"
     CDS             complement(1100..2722)
                     /locus_tag="IW662_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0865685.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2971..3492
                     /locus_tag="IW662_000843"
     CDS             2971..3492
                     /locus_tag="IW662_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865686.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3678..5408)
                     /gene="spxB"
                     /locus_tag="IW662_000844"
     CDS             complement(3678..5408)
                     /gene="spxB"
                     /locus_tag="IW662_000844"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ0865687.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5573..6391
                     /locus_tag="IW662_000845"
     CDS             5573..6391
                     /locus_tag="IW662_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ0865688.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6494..6691)
                     /locus_tag="IW662_000846"
     CDS             complement(6494..6691)
                     /locus_tag="IW662_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865689.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6860..7438
                     /locus_tag="IW662_000847"
     CDS             6860..7438
                     /locus_tag="IW662_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0865690.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8483..8986)
                     /locus_tag="IW662_000848"
     CDS             complement(8483..8986)
                     /locus_tag="IW662_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0865691.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9344..10369
                     /locus_tag="IW662_000849"
     CDS             9344..10369
                     /locus_tag="IW662_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0865692.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10692..11570
                     /locus_tag="IW662_000850"
     CDS             10692..11570
                     /locus_tag="IW662_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865693.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12046..12162
                     /locus_tag="IW662_000851"
     CDS             12046..12162
                     /locus_tag="IW662_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0865694.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13090..13428
                     /locus_tag="IW662_000852"
     CDS             13090..13428
                     /locus_tag="IW662_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865695.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13522..13752
                     /locus_tag="IW662_000853"
     CDS             13522..13752
                     /locus_tag="IW662_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865696.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14152..14523
                     /locus_tag="IW662_000854"
     CDS             14152..14523
                     /locus_tag="IW662_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0865697.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14797..15489)
                     /locus_tag="IW662_000855"
     CDS             complement(14797..15489)
                     /locus_tag="IW662_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0865698.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15492..16277)
                     /locus_tag="IW662_000856"
     CDS             complement(15492..16277)
                     /locus_tag="IW662_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0865699.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16514..17914
                     /gene="lacG"
                     /locus_tag="IW662_000857"
     CDS             16514..17914
                     /gene="lacG"
                     /locus_tag="IW662_000857"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0865700.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17939..18256
                     /locus_tag="IW662_000858"
     CDS             17939..18256
                     /locus_tag="IW662_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0865701.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18269..19942
                     /locus_tag="IW662_000859"
     CDS             18269..19942
                     /locus_tag="IW662_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0865702.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19956..21587
                     /locus_tag="IW662_000860"
     CDS             19956..21587
                     /locus_tag="IW662_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ0865703.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22078..23643)
                     /gene="guaA"
                     /locus_tag="IW662_000861"
     CDS             complement(22078..23643)
                     /gene="guaA"
                     /locus_tag="IW662_000861"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0865704.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23702..24625)
                     /locus_tag="IW662_000862"
     CDS             complement(23702..24625)
                     /locus_tag="IW662_000862"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0865705.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24821..25426
                     /locus_tag="IW662_000863"
     CDS             24821..25426
                     /locus_tag="IW662_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0865706.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25734..26750
                     /locus_tag="IW662_000864"
     CDS             25734..26750
                     /locus_tag="IW662_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0865707.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            26891..28192
                     /locus_tag="IW662_000865"
     CDS             26891..28192
                     /locus_tag="IW662_000865"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0865708.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28238..28900
                     /gene="deoC"
                     /locus_tag="IW662_000866"
     CDS             28238..28900
                     /gene="deoC"
                     /locus_tag="IW662_000866"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0865709.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            28921..29319
                     /locus_tag="IW662_000867"
     CDS             28921..29319
                     /locus_tag="IW662_000867"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0865710.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29385..30467
                     /locus_tag="IW662_000868"
     CDS             29385..30467
                     /locus_tag="IW662_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0865711.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30679..32241
                     /locus_tag="IW662_000869"
     CDS             30679..32241
                     /locus_tag="IW662_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865712.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32234..33367
                     /locus_tag="IW662_000870"
     CDS             32234..33367
                     /locus_tag="IW662_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0865713.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33367..34323
                     /locus_tag="IW662_000871"
     CDS             33367..34323
                     /locus_tag="IW662_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0865714.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34422..35588
                     /gene="deoB"
                     /locus_tag="IW662_000872"
     CDS             34422..35588
                     /gene="deoB"
                     /locus_tag="IW662_000872"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0865715.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35612..36430
                     /locus_tag="IW662_000873"
     CDS             35612..36430
                     /locus_tag="IW662_000873"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0865716.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36447..37154
                     /gene="deoD"
                     /locus_tag="IW662_000874"
     CDS             36447..37154
                     /gene="deoD"
                     /locus_tag="IW662_000874"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0865717.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11630 a         6509 c         7891 g        11259 t
ORIGIN      
        1 attttgatta gaacttgcga aaataaaccg gttaaagtgt gtctagcgca agagatttgc
       61 tataatagac gataagggta aaatgtaacg agaaggggtt gaaaaatgtg tatcaaattg
      121 taacagattc ttgttgtgat ctgccatacc atgtattagc aaaagaaaaa gttgattttt
      181 tatcaatgaa tctccaattg aatgatcaag agttagtaga tgacctcgga aagacattca
      241 attatgaccg ctacatgaat cagctgaaaa atggtgcgat gcctacgaca tcacaaatca
      301 atgttggccg atatttagaa tttttccgtt cttatgcgga aaaacagatt cctgttttat
      361 atgtggcttt ttccagtgga ttaagcggct cttacagtag tgcgcttcaa gcggttgaat
      421 tgctgaagga agaatatgaa gaccctgaga tctatgtttt tgatacaaaa gctgcaagta
      481 ttggggaagg gcttttagta ttggaagctg taagattgaa aaaagaaggt aaaagcatcg
      541 aagaaaccct agagtggctg gaaagtaatt atttgaaagt ccattcatgg gtaacagtag
      601 acgatctgaa atatctagaa cgaggcggta ggatttccaa agcaagtgct actcttggca
      661 gtctattgag tgtaaagcct attattacgg tggataaaga aggaaggctg acgaatgttg
      721 acaaagttcg aggaagaaac aagtcgatcc agacaatcgt caatgagacg gtaaaaacaa
      781 tcgttgcacc acttgatcaa atcgtttatg tggcttacgc tggggatcta gagagcgcgg
      841 agaaggcaaa acggcttttg gaagagcaga taaagatgaa agatgtgaag ctttatccct
      901 taggaccaac gatcgccagt cacacaggct atggctgtat cgcgattttt tcgatgggaa
      961 tcagtcgata aaagcagtaa ctcattctgt aaaaaagccc agaaattgat tgtaacaaaa
     1021 acagcgactc ttaaaagata gatgagcatc ctatctctta gaggtcgctg tttttttatt
     1081 ttttaagcta aataggccat tatgcccata actcgttcaa ttgtttttga atatctgcat
     1141 tttccaaaaa ttcatcatat gtcgtatcag ctcgatcgat cacaccttta tccgaaacgg
     1201 caatgatgcg gtttgccaat gtttggataa attgatggtc atgtgacgca aataagattg
     1261 agccagtaaa tgccatcaag ccgtcattca atgctgtgat tgattctaag tctaaatggt
     1321 tcgttggatc atctaataca agtacgttag ctttcgaaag catcattttt gaaagcatgc
     1381 aacggacttt ctctccacca gataaaacat tcactggttt caatacttct tcaccagaaa
     1441 acaacattct tcctaagaag cttcttaaga aggtgttatc atcttcttct ttacttgcat
     1501 attgacgcaa ccagtctaaa atcgttagat cagtatcgaa ttctttcgtt gtatcctttg
     1561 gtaaataaga attacttgtt gtcacacccc aacgtactgt tccagtatct ggcgtaattt
     1621 cacccatgat aactttgaat agcgttgttg ttgttatatc attttctgcg acgaaagcga
     1681 ccttgtcttc tttgctcaaa ttaaaagata tatcatctaa aattttcttt ccatcaatcg
     1741 ttacacttac attttctacc tgcaataagt cgtttccaat ctcacgttca ggtttgaatc
     1801 caacgaatgg ataacgtcta gaagaaggtt ggatatcgtc taaagtaatt ttttcaagca
     1861 tttttttacg tgaagttgct tgttttgatt ttgatgcatt cgcactaaag cgggcaataa
     1921 attcttggag ttctttgatt tgttcttctt ttttggcatt tgcgtttgct tgaagtttag
     1981 ccgctagttg actagattcc aaccaaaaat cataatttcc tacataaagt ttgattttac
     2041 caaaatctag atccgccata tgtgtacata ctttgttcaa aaagtgacgg tcatgggata
     2101 caacgatcac agtattttca aaattgatca aaaattcttc cagccaattg atcgattgga
     2161 tatccagacc gtttgttggt tcgtctagca aaagaacatc aggtttacca aatagtgttt
     2221 gtgctaataa cacttttacc ttttgtccac cagttaattc actcatttgc tgatggtgca
     2281 attcttcagg gatgttcaga ccttgcagta aaacagctgc ttctggttca gcttcccatc
     2341 cattaagctc agcaaattct ccttctaatt cagctgctcg aataccatct tcgtcagtga
     2401 aatcctcttt catgtaaatc gcatcttttt ctttcagcac ttcatacaga cgcttgtgtc
     2461 ccatgatgac tgtttcaagt acagtatagt cttcatagtc gtaatggttc tgtttcaata
     2521 cggccatccg ttcatctggt cccatagtaa cggaacctgt ggaaggctgc agatcccctg
     2581 ataacacttt aaggaacgtt gatttacctg caccatttgc accaatcaag ccatagcagt
     2641 ttccgggggt aaatttaata ttcacatcat caaatagttt gcggtctgaa aagtgcaaac
     2701 tcacatcatt tacagtaatc aatttttttc ctcactttta tcattattag ttttgacaag
     2761 tcttatagaa ttatataggg gaaaggtaaa agattcaagg caatcgaaca ttctttgtgt
     2821 ttcacgtgaa acagaatgaa aaaaataaaa ataaaagcgg agcgaaaata gctactatag
     2881 gatttattag tttgcaacag cataattttt gacgttattt ataagaggga gtactataaa
     2941 actgtaaaag aaatgaaaga aggattgaaa atggatgaaa aaagaaccat cgactggggc
     3001 tcattgattt tgggaatttt atttgtactt gtctcattgt tatcatttca agaccctgtc
     3061 ggaaatttag ttgcaattgt agtagtcttt gcgatttttg cctttattaa agggatattt
     3121 gaactgtttg tacggaacag attaaaagag ctaacaggat ataaaggaaa gatgccctta
     3181 ataatcggga tcatcgatat tctagttggc gtctttttct tattcaatat tggtgcagga
     3241 gtcgcagcat tgccttttgt ttttgcagta tggtttattg ctgattcagt tttagcgtta
     3301 tttactgctg acttggcaag aggagtaagt gaaggatatt attggtttac gatcatcgtc
     3361 aatattttag gaattttatt aggaattttc ttattgttta atccaatttc ttctgcgttg
     3421 acattaagct ttttagttgg attctatttt atgttatttg gtatcacaca tatcgtctat
     3481 gcatttagat aagtaaaatc aaacaaataa aaaagccggc ttccgattac agttttactg
     3541 ttgtaattgg aagccggctt ttttatcatg gacaaatatg cacgaggtga tttttaggtg
     3601 tcgagacaaa tttatgcaaa aatgtgagaa gctatataca caaattttat cttaatactc
     3661 gaagcatagc gcagttgtca caacctcttt tcatataatt cattcaatac tggcaaatca
     3721 tggacttcat atttttcttt gaatgcttgg atttcttctt cagaatattt tcttggatct
     3781 aaatgcagat cttctactgg caatgggcgc ttgttattga tcttcacatc gattactaca
     3841 ggacgatctg attttgcagc tgcatcaaag gcaaatcgaa gttgcgagta ttctgtgact
     3901 gtaaagctat ttgccccgag cgcttctcca actttaccga aatctgcatc ttctaaaaat
     3961 acaccaaatt tcttttgttc cgtatcttct tgttctgctt cgatgaaacc aaaagaatca
     4021 ttcgaaaaga caacattaat aattggaagc tgatatttga cttgtgtaat gatatcttgc
     4081 atgaccatag caaacccgcc atcaccactt aaggtaaata cttgtttttc tgggtagctt
     4141 agttgtgcgg caattcctcc tggcacacca ttacccatag ttgcgaacca tccagatgtc
     4201 gtatgttttt gtttaccgtt catattcaat aaacgaatag aatggatagt cgtgttaccg
     4261 acatctgtga cgaaaatcgc atcgtcttca gcaatacgat tgatttcttt atagacagcc
     4321 tctggacgaa tcggttcttc ctctcgatct tcaaaagatt tcaaccaatt tacccatttt
     4381 tctttatttt cttgatttgc taggtaccag ctatcagcag ggcgagcatc ccctaactcg
     4441 acaagtcttt gcaaagctgt gtttgcatca ccgaggatcg acaaagatgt ttcatggcgt
     4501 cgtccaaatt ttgacgcatc gatatcgatt tggataaatt cagcagttgg attaaaaaat
     4561 gcacgtccaa atgggaagtc gctaccgaca aataagatca gatcggcttc tgccaatgct
     4621 tcatttccgg gtttagtagc gacacgtcca gcaaaaccaa gaaagttttc ataatcatca
     4681 ggaataattc cttttgctag aacggaagcg acaacaggaa ctgaaaaatg ttctgagaat
     4741 tctttgattt gcggaaatcc gtttcgcgtt ccttgaccaa cataaagaac tggttttttt
     4801 gctttttcaa gataaggcaa agcagctagc aaatcttttt cttctggtaa tatcacactt
     4861 gttttgtgag tgtgagcggt ggagaaaggt tgttcgtcaa tttcttcaaa accaagatca
     4921 acagggatag tcacgatagc cacgccttta tgctcatagg cggctttaat ggcttcatct
     4981 actacatgcg gaagactttg tggtgtcatg actgtacgat tgtatacgct aacatctgcg
     5041 aaaataggat tttcatttag ttcctggaaa gagttatagt tcatggatgt agaagcaact
     5101 tgtcctaaga acgctactag aggcacatgg tccatctgtg catcatacaa accattgatc
     5161 aaatgtgtcg caccaggtcc tgcagagccg aagacagcgc cgactttccc cgttaatttt
     5221 gcatctgcag cggctgcgag tgcgccgact tcttcatgac gaacttgaat gtagttgatt
     5281 tcggtttttt cttgatatag cgcatccata attgaattga aagatcctcc gggaatgccg
     5341 taaatatggt cgatacccca gctttcaaga acttttacca ttgcgattcc tgcattgatt
     5401 tttgacatgg tgtttcactc ctttagcaat gatgatacat aaattttttg tatctagagt
     5461 atagaacttt ttatcgaaaa tcccaactaa ttcgtctttt cacaaaaatg tagtcatgtt
     5521 tttgggaata tgctagagta gtacatagat agattcaaga gaggggaatg gcatggttga
     5581 atggctattg agacttgaac cttggcagca agcacttgct gggacagctt ttacttattt
     5641 tatgacagct cttggtgccg ggttggtttt tttctttaaa gaaatcaaaa aagaagtgct
     5701 aaacttgatg ttaggatttg cttctggtgt catgattgct gcaagttttt ggtctttgtt
     5761 agatccggct attacaaaag cagaagaaaa tggagatatt gcttggctag ttgtaagtat
     5821 tgggtttggt ttaggcggtg tgtttttata tatggcagat aaaacactgc cccatatgca
     5881 ttttggacca caacatgaag cagaaggatt gcctacccat ttaaaacgga cgatcttgct
     5941 tgttttttct attacattgc ataacattcc tgaaggttta gccgtggggg tagcattcgg
     6001 tgcagcagca actgcggata atccgacagc cgctatatta gccgctgttt cggtggcgct
     6061 aggtattggt atccaaaact tcccagaagg agcagctgta tcaatcccgt tgcgacaaga
     6121 aggtctgagt cgtaaaaagg cctttgttta cggacaagct tctggaatcg ttgaaccaat
     6181 tgctggagtg attggagcgc tactagtaac gaaagtggag ctgctgcttc cttatgcact
     6241 tgcttttgcc gcaggagcaa tgatttatgt cgtggtggaa gaattgatcc ctgaagcaca
     6301 gcagacattg tccagcaaac gtcattttgc agtttttggc gtcatgagtg gatttatcat
     6361 tatgatgatt ttggatgtgg cattaggata aataaaaaaa atatatatga tatatcaaat
     6421 agggccgcga acttatatcg cgaccctatt tttttagtaa gttatatcat aatgaagtga
     6481 atggtatcat acattaataa tagttttttc tatgtgtatt tcctttgatc cagcgaacaa
     6541 gccagatgat aagtgcgatt gggattagaa ctttgaccgc aaaccataaa agcccgctta
     6601 ggacactgac aaaaagactg atgacgaagc tagctgcaat tagcactaaa agaaccatga
     6661 agatatttaa gaacatattt ttttgacgca tacaaaaccc tcctcttgtt cattctttac
     6721 tatagaaaaa gtataacagg gattgtttaa aaacacctcc aactgaagac tgatttttac
     6781 ttcatctact taaatgatca aacaattgct ttttctctgg tttatcatct aaaatagagc
     6841 ggaaaaggag gagaatatca tgcctaaaaa tcgtcttgaa gcatttacag atgctgtaat
     6901 cgcgattatc atgactattt tagttttaga acttcatgaa cctaacggag acacgtggga
     6961 ggcgctcgct gcattaggtt accgattttt tatctatatt gtcagtttta tcattttagc
     7021 gatttattgg aacaatcatc atcatttatt tcaagttgta cataagataa atggacgggt
     7081 cttatgggcg aacagtttct ttgtttttgc gctttcgctt tttccgtttg caacttcatg
     7141 gatcagtgag tacatcaata gcctggctcc agaaatgact tatggttttg ttaccttgac
     7201 cgctaatatt gcgtattata tcttggcaaa agaacttgtc aaagcaaata aaggccacga
     7261 gaaagcattg aaggcgatgt tgaataatta caagaaatcg tatctatcga ttggtttgaa
     7321 tgtgttagga attgtcttag ggtatttagt agcgccagta gccgtgctga tcgcaaacat
     7381 cgtgattcta gctgcttgga tcatcccaag tcgtaccatc gaatcacaat atcggtaaaa
     7441 tgatccgtgt ttaaaattta agagaaaaat atagaaaaaa taaataaacg atcaaatatc
     7501 cgagtgatac tagtattcaa agagggatgc agatggatca aaaaagaatt catgtaggac
     7561 tgattactag ttttgtcacg atgcttattt atttggatca aggtcatttg acttggacag
     7621 tagttggtgt cgtaggattt ttgacattat tcacagcaat aaaaaaataa acatctttat
     7681 gtaatctgat gttctatcta tttaagaaaa gcatgtttac agcagtatga ttctgttgta
     7741 aacgtgtttt tatttgtgga aaatttaatt tttagaaaag tgtatcgaaa aatagatagt
     7801 tagtttttcc acagtggata acttcgtaag agataaaaat aaaaaattag tcaaagaagg
     7861 gcgtatagat ttcttttgat aggagatata caacagaaat gaataaatat taacgtgttt
     7921 tcaaacagta taaaacggtg tggtttttgg aataaaaaaa taacatatct cattttacac
     7981 ttttgttatt ttatacttaa aaaataacga cacagaaaat accgtgtctc agatttctct
     8041 actacttcta aaaactactg tttttttcct ttgaaatgcc ttcaaaaagt aggatcaggc
     8101 agaatatact ggcagaatca aggagaaggg agcctaaata aggagaaaaa ccatagaaaa
     8161 tataaagtaa ttacttataa aacaagcttt ttatagaaaa ataatatacc tgagtagggt
     8221 ataaattaag acggaattcc tttctagatt gagatattga ctaggaagaa tgttaagatt
     8281 ttaagccttt aagctaaaaa aaaacccttt ttgttaaata aggggaatat agcctgtcag
     8341 tttcaaactg caagtcgtcc tgctgcaata atatagtaga ttagagatga aaatagattt
     8401 ttttaaacca atgactgaat aaaaagaccg tcatagatac acattggaat gtgtctatta
     8461 cggtcttttt ttacgaacga ttttattgaa gacggacagt tcgggtcaaa aataaaaaag
     8521 ctacgatcag aaataagaat aaaatgtatc ccaaaataat ggctagacgg tctaacttat
     8581 ttgtcgatag atgtttgttt agaagttgat aggcccataa acctatcagt cctcctgttg
     8641 tattagttat caaatcagtg atgtccgcta cgccaatacc gaataggaat tgaatcaatt
     8701 ctgccaagaa gcttacaagg aaaataatcc ctaatttttt agaaaaagaa agtctcttga
     8761 aattgacatt caaaagcacc ccaaaaggaa aaaagacaat caagttataa gtggtctcac
     8821 gtaataccgt cgtattttca aaagaaaaag tagaaaaagg gacgagattg acattgctgt
     8881 aagaataagt gagaaattgt ggaagattaa atgatagctt gaataaaatc aaccataata
     8941 gaaagaataa ataaatccct aataaaaatt ttgatacata ccgcatagac atctcctcca
     9001 tttaaaaagt ataccatcga aaaccgctga aaaatcataa aaaagtatga agtttaagga
     9061 ccaacgtagt tatcgtacga aaattagaag ctttttttgt tgggaatccg gtttctttaa
     9121 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
     9181 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     9241 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     9301 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     9361 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     9421 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     9481 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     9541 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     9601 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     9661 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     9721 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     9781 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     9841 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
     9901 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
     9961 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
    10021 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
    10081 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
    10141 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc
    10201 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
    10261 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
    10321 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
    10381 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
    10441 aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa
    10501 aaattttata tttattctat caaaaaattt tgtttatgat ggtaactgaa taaactttcc
    10561 tgagatggtc aatgctttcc ttgaaagtct tacagatatt ctttcttcat agaaagttta
    10621 agttttatag ttagtgtaca ataaaagtat gtttatacca tatggtgtcc ggataatcag
    10681 tcatttaaac aatgaggttc aaagatatga aaaggggaaa tttgatgaag aaaataagca
    10741 atgaaatcca aattctggca gaaactttaa ctcttcccaa agtcgttata gagcaaatga
    10801 ttgtacaagc aaaaaacatc cttgatgagg tgtttgagca atatagtcat gaactaatgg
    10861 atcctaataa agcaaaggcg actgcaaaga aaatagacaa agtctatgaa gaggaacctt
    10921 ttgcagcatt agctatttat ctatatgcag cttctcaaag ttggaaaaaa atttatcaac
    10981 cattgaagat tccaagaact atctatttgg caacaatgaa tgcttttact agatttattc
    11041 atgaacattt ccaagtaaca ggaagctatc gctttgaccg tggtttttgg atttggcatt
    11101 acactagtgg cttgatcttt cggattggtg aattggagta cgaaatgaca tattttccga
    11161 aagggtacac ggatctaaga ttagaaggaa agcagtgcct ttctcttcat attccttccg
    11221 atgcagattt atctcagaat aagataagag atagttacga aagtgccatt cgtttttttg
    11281 aactttattt tcctgattac cattaccaag tgatgtacac agatacttgg ttactatcac
    11341 ccaatcttac taaatggttg aaaaaagagt caaaaatctg tctttttgca gccgattatc
    11401 gattattatc agttgatgaa caggacgata gtggagtccc ttggatcttt ggacgagtag
    11461 atgcccaaat acatgattat ccagaaagta cgagtttaca gcgacaagca aaagagcaat
    11521 tgcttgcagg cgagcacatt ggctctggct taggtattct tacttcataa gtttagtact
    11581 tcgaaatagt ttaaaagagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa
    11641 acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa
    11701 atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc
    11761 agcctctttt ttagtgttct atcgaacaca cagtacgttc aataggtaaa ctagctataa
    11821 caatagatat taaagttaac ggacaagagg gcctttttta ggtagctaaa acgtaataat
    11881 gaaaatttat ttccagagat aggagtcaat cagctagtct gatcgactgt tttaacaagt
    11941 tgactttcat tttaactttt gataccattt aaaacacaaa tcgtaattgt ttcgatttgt
    12001 gttttttagc atgactacaa tcaaagaaga aagtgggtcg tccatatgga aaaaacagat
    12061 atctcaagtg cataccgccg cttgaaaagc ccaaatatca aaacacgaaa acgtgcgctg
    12121 aagatcatca aagatgtgaa acggaatagt gggaaaaggt aagaagaagg aaacttaatt
    12181 aaaaaaatag ccgaaaaacg tttttcggct attttcaata atcggttttt gtgtaaggtt
    12241 tccgaaagaa ctgctgttga ttttccattt ttaacatatg ataaattggt agaatctttc
    12301 ttttcaaatt gataccattc cctaattcca gttatatagt taactacaga accacaaagc
    12361 acgtacgttt ataaaagtat tatctgtagt tatttttcaa ttccctttca acctcttatt
    12421 ttgaccagta gtattgatca ctttaccttt taacaccttt gttcgttttt gaaacatttt
    12481 tagtcaagaa tggaaacgta actacataat caaaggtttt gcgaaatatt tcatgaaata
    12541 tttcatgtag aagcgagcaa tacggttgca aataaccatt atcgtttaat aagcagttac
    12601 gaaagctccc aatatctcta acgttcagct cactccattt cttttcttca aaaaaatcag
    12661 tgattcgtat ctctttctat ttgtcgaaaa agttaacttt aaaaagtaac gccgaaaaaa
    12721 tatgcaaaaa aataaaaaag cagttactat tgctagaata acatcaagga ataataagtt
    12781 ttggttttca gataatagtc gataagttgt gtattggaat tttagataca gtaataaagt
    12841 atttttcatg aaattttgtg agaatcacta ttacaagtat ctttaataaa attaatggtt
    12901 aaggaggaac ttaaagtaga atgtaaatga atagttgtag tcagtcgatt gtgatactag
    12961 actcaaagtt gcaggaataa gtttttcaat ttgtgattgg aagataaaaa atgaaaaaga
    13021 agggcttttt tttatacttt gttgattgta gtgtttattt tccctctgtt tagccaagta
    13081 acattttcta tgactcaacc agtttcaagt gaggaacatg ataacctttt tcgtgctaca
    13141 gaagagttta agaaatatca ggaaaatatt gttccggaga ctccattagc tagtgagcca
    13201 attactgata ttgatggaaa ccaaatagga tggtatatac agtacgaagt tatggtggta
    13261 aataatgact caatagtaaa aaatacagta ggcttagagc taacaccgat tgaaaaggtg
    13321 agttcattgt taacgtatat ttatactgat gaaagtgacg agactgagat cattttaacg
    13381 gattactcaa aaattgctac tgataaaaag tatatattgt ggatgtagac actagtgaaa
    13441 tactttatga tatagaaatt gatgaaaatt tactagatgt agcacaagaa atcgatcatc
    13501 aaaagcagga agctattcga tatgctggta gaaaagggaa aagaaaatat ttattacgtg
    13561 aatgtagcaa aagttagaga agatgaaaat gagtggaaag aattcaagag tcgctactca
    13621 attaactcga ctccgacatt tacagtttac agagaaggat cgatagaaaa aactgttttt
    13681 tggactaaag aaagtggaat gtctttagct gaagtagaag agtttttgga ttatgttagt
    13741 atgcaacagt aagggagttc ttattatgaa aaattttgac acatattatg ttattgtttt
    13801 ttcgatatct ttgctgttaa tattactaac tttaatgcag tataaagagt atccagagta
    13861 ttttatttta acttttgtat atttagtaag tagctacaca cttctatata aattcagaag
    13921 ttcttctaga gtttctaaag tttgtttctt aattagctta ttgattctat aatatttttg
    13981 atttatatag gaaattaaaa atattataga atcaacactc agagaacggg ttggttttta
    14041 atagatttaa taagtactga taatgaaatt tctcctggtt gggaaaacga agttggttac
    14101 cctgtagaag aattttttca agattacttt gcggagtatg ccgataggat aatgagagat
    14161 tacgtaacag ataaaactca agaagtcatc aatcaatccc agtcattaaa tgaattatct
    14221 gataaggcga aagaagaagc agttgccatt atttcttcag ggattagtga ctatattgat
    14281 atgagtggtg aaatcaatta tggcgataaa gacaaaattg tacgcatgat aaaagctttt
    14341 atcaaaactt ttgatgtgaa ggcagatctt aagtttaatg atacttacct agtaggaaaa
    14401 ttaattaata tccttgggac agagaaagat acggaaacgt ttatttcctc tttgtctatg
    14461 ttgtttacag ataagcattt ttcaactcag cttggtggag aggaactagt ggaaatcttt
    14521 taaccattac gcccagcatt aataaaagta tatcaaaaaa gtactcctga tgatttttaa
    14581 gattggtttg agaaataatg tgaaaacact atttttaatg ttaattatag atagagatga
    14641 acgcgtcata tggttaaaag aatgaaaaag tcaaagatac actgctatat ttcactaagg
    14701 atatagcagt gtatctttca tgtcgtttca ctggcagttt tttataaaag gaggtgtgca
    14761 tttcagttgc ttttctttgg tagttagttg aatcgtttaa gaattaacgg atgtggcaga
    14821 caggtgctct gtccaaaaac cagtaaaatc tttgatggtt ccatctttat ttagctgtgc
    14881 ctgttcaaat actgttttgt ttttaccgaa tggatcaatt gcaataattg ttccgatccc
    14941 cgcacgtttg gcagcaagta atcctgagta agcatcttcg ataacaagac aatcagccgg
    15001 agctaagctc agtttgtctg ccgctttaag aaaaatatcc ggttgcggtt tcccagggaa
    15061 agagccgtcg tcatagacaa tcttgtccca gtcaaaccag cgggctaaat cgaaaatatc
    15121 aaaataaaaa gcaacattct ctttgacagt agccgtggca atcgttaatg gaatctgttg
    15181 gtcagtcagc tggtcaagag tcgcttcaag cccttttgtc aactgaagct cgttttcatt
    15241 ttgcaagcaa agcatacgat aatagctttc tttttcttct gacaatacgg cgatctcaga
    15301 ggcggtcaaa tgctctgaaa tgaaatgttt caaaatctcc gtattcgtcc gaccatgaat
    15361 attttttaaa atatcctcgt ctgataaacc atcttttgta tgatcatgta tcatatgcag
    15421 ccaagcagct tcatgcagat gagagtccaa aaacattgtt ccgttgaaat caaatataat
    15481 ccctttcata gttacgctcc tatagtggta ttttcggttg aatcagcaat ttgcagatcg
    15541 acatattgtg catagtgctc ttgaacctcc cgcgagatct ggcgatccgt aatgagcaga
    15601 tcgaaatcat gcaaattata aaacacataa aaatcatctc gattgaattt tttattatca
    15661 acgagtagat atttcttacg ggaattattt agagcgatct gctgtgcttc tccttcttcg
    15721 gtactgtaag tagagatcgc ttcgttgtga acgccatttg aactgataaa tgcttttgta
    15781 aactttagtc ttcttaaatt gtcgttggtg atcggtccga caaatgcgcc ggtatttttc
    15841 cggaaatcac cgccaatcag tagaatttct ggtccatctg cttgagcaag aatatcaaaa
    15901 actggataat tatttgtaat gatccgaatt ttcttgttca gtaaatgcgt cgccaatagt
    15961 tcgattgttg tacctggtcc taagaaaatc gtttctccct cgtgaatcaa ggaggctgct
    16021 gtggcagcaa tttctttttt ttcttgaatc tgcagagtgg ctttttcagt gtgagaaagt
    16081 tcttgatcta aagtgtagga aagactttga gcccctccat ggacacgcag cagcttgccc
    16141 gatttttcta attcatctaa atctctgcga acggtcatat cagaaacatc taattgattg
    16201 atgatttcat taacggtaag gattcctttt ttattgacca tttctacgat agtaagcaat
    16261 ctttcttttt tcaacattgt cttcacctct aatttaagga taatctatca tataaacgga
    16321 caacagtcaa tctaattcga acaaaagtaa acatttaggg tggtttttat gtttgttaca
    16381 atagatttta ttgctgtttt ctggttttta tgattatttg tgtttgtttt tgttgacaaa
    16441 tgtttcggat gcgtttacaa tgcaatcagt tagaaagagc gaaaactgat tatagatttg
    16501 aggagagatg tacatgctta aattaccaga agattttatt tttggcggag ccacagcagc
    16561 ctatcaggtt gaaggagcca ctaaggaagg tggcaaaggc gctgttgctt gggatgactt
    16621 tttagaggaa caagggcgat tcagtcccga tccggcaagt gatttttatc atcaatatgc
    16681 aaaggacatt gaattgtgtg aacgttttgg tgtaaatggt ctaagattat ccattgcttg
    16741 gagccgcatt tttcctgatg gagctgggaa accaaatcca gaaggaatcg ctttttatca
    16801 tcgtgtgttc gaagaatgta agaaaagaaa cgtaacgccg tttgtgacgt tgcatcattt
    16861 tgatacccct aaacgtttgt ttgaccatgg agattttttg aatcgagaaa caatagaagc
    16921 atttgtttcc tatgccattt tttgttttca tgaattcaaa gaagtcaagg tgtggagtac
    16981 ctttaatgaa atctatccag ttgcaaccaa tcaatatttg ttaggtgttt tcccaccagg
    17041 aatcaaatat gattttacaa agatcgtcgc ctgtcttcat aatatgatgg ttgctcatgc
    17101 ccgtgtagtg aactatttca aagaaaacga acttccgggt gaaattggtg tagttcactc
    17161 attagagacg aaatatgcgg caacagacgc gccggaagat aagcatgctg cctttttaga
    17221 cgatgcattg tcgatccgtt tcttattaga tgctacctat ttaggctatt attcaactga
    17281 aaccttgact gctcttgatg agatatgtga agcaaatcag gccagctatc attttcctga
    17341 agaagatttc gttgaattga aaaaagcatc cacgcgtaat gactacttag ggatcaatca
    17401 ctaccaatgc cattttgtca aagcttacga tggagaaaat gcaatccatc ataacggtac
    17461 gggtgaaaaa gggacctctg tttataaagt gaagggaatc ggagaacgta tttataaaga
    17521 aggaattcct cgaactgatt gggactggct gatttatcca gaaggtctat atgacttgtt
    17581 gctgcgaatt aaaagcgatt acccacatta caacaaaatc tatatcactg agaacgggat
    17641 ggggtataaa gatcagtttg aagatggcat tattatggat cagccgagaa tcgactattt
    17701 gagagtctat ttggaatcct taagcaaggc gataacagcc ggggtaaatg tgaaggggta
    17761 ttttttgtgg tcgcttatgg atcttttctc atggacaaat gggtataaca aaaggtatgg
    17821 cttgttttat gttgattttg aaacccagaa gcgttaccca aaagaatcag cttactggta
    17881 caagttggtc agtgaaacca aaacgattat ttaataagtg ataatggagg aagaaaagat
    17941 gaataaagag gaattacaaa tgcttggttt tgagatcgtt gcatattcag gcgacgctcg
    18001 cagtacatta ttgaaattat tgaaagaagt aagacaagga aactttgaac atgttgattc
    18061 ggcgttgaaa gaagccgacg aaaatttaac ccttgcacat aattcgcaaa caaaaatttt
    18121 agcggaagaa gcatctggga aagagatgga gatgggattc atttttatcc atgggcagga
    18181 tcatttaatg accactttat tgctgagaga tctgattcaa gactttattg tactgtacag
    18241 acagaatcaa ggataggaga agaacgctat ggatggaatc attaaacaaa tcgaaaaagg
    18301 gaaaccattt tttgaaaaaa tctcacgtaa tagttattta ggagccgttc gtgacggatt
    18361 tttaacagcg atgccagcta ttttattttc aagcattttc attttgattg ctgcggttcc
    18421 tgagattttt ggttaccaat ggcctgaatc tgtctccaca tggctatgga aagtctataa
    18481 ttattcgatg ggtgttgtag ggttactcgt ttcagcaacc actgctcgct gcttggctga
    18541 ttcgatgaat cgtcgcttac cgaaaaacaa agtaatcaac accacctctg tgatgctggc
    18601 ctctgtggta agtttcctgt tgttaagcgt ctctccgatt gaaggtggat tttcaacgga
    18661 atatatgggg acaaagggac tactggcttc ctttgtcgct gcatttttaa cagttaattt
    18721 atataagttt tgtgtgctga aagatattac cattaaaatg cctaaagaag ttcctggaac
    18781 gatttcgcaa accttccgag atatttttcc tttctctttt tcagtatttg cggccgtttt
    18841 aattgattta ggcattcgac aaatcttcca aatctcattt gcagaagcaa tcattacggt
    18901 gctgcagccg ctatttacag cagctgacgg gtacttagga atcgctatta tctggggagc
    18961 gatggcgctg ttttggtttg tcggtgttca tggaccgtcg atcgtggagc cggcaattgc
    19021 ggcaattatc tatgccaatg tggaaaccaa tctgcaattg ttcaattccg gtgaacaagc
    19081 ttctaaagta ctgacggtgg gtttagggaa ttttgtagga actatgggag gaactggggc
    19141 aacattggtt gttcctttta tcttcctact ctttgctaag tctaagcagt tgaaggcagt
    19201 tgggaaagct tcctttattc cagtaagttt cgctgtcaac gaaccattgc tatttgcggc
    19261 acccatgatt ttgaaccctt atttttttat tccatttttg ttaacacctg ttgtaaacgt
    19321 ttgcctgttc aagttttttg tcgatgtatt gggcatgaac agctttatgt acgttttgcc
    19381 ttgggcaact ccagcaccga tcggtttggt tctaggaacg ggcatgggta tcttagcttt
    19441 tgttctagca gcggttctaa tcgtcgttga cttcctgatc tatctaccat tttgcaaagc
    19501 atacgatgct gtcttagtag agaaagaagc acaaaaagca gaggtgatcg caaccgagga
    19561 aggtgcttct gccgatgtac gtgaggaagt acctgtggca gcgagtcaag ttatcagcga
    19621 tgcaaataaa gaaatcaatg tgttggtact ttgtgctggc gcagggacaa gtgcgatgtt
    19681 agctaatgct ttaaaaaaag gtgcatctga atcgaaagca ccgatcagtg caatggcggg
    19741 tgcttatggt tctcattacg aaatgctgca ggattttgat atggtggtct tagcaccgca
    19801 agtcaattct tactatgatg atatcaaaga agacaccgat cgcttaggaa tcaagctggt
    19861 tgcgacaaaa ggagcagaat atatcggatt aacgagagat cctgaaaaag ctgttcagtt
    19921 tattctgaac caatttcaat aaaggaggag ccataatgaa aatgggaatt tggttgcgta
    19981 aagctgtttt atttgcagct gtgttagggg ctttgggggc tgtttcaaaa actgttgaag
    20041 catctgccag cgaatttatt cgcggagcag atatttcgat cctgtcagat atggaaaaat
    20101 ctggtgcaaa atattatgaa gacgatattt caaaagatgc cttagagatt ttaaaaaata
    20161 atggtgtcaa ctatgttcga ctgcgtttgt ggcaggatcc ttataatagt aatggagaaa
    20221 gttatggagc agggacaaat gatctggaaa cgacgattga ccttgcaaaa cgagcaaaaa
    20281 atctgggact taaggtgctg ctggattttc actacagtga tttttgggtt gatcctggga
    20341 aacagaatct tcctaaagca tggcaaggtc tgacctttga agaaatgaat acggcattgt
    20401 acgactatac aaagaacgtt ctgtcggaaa tgaagcagct ggacgtttat cctgatatgg
    20461 ttcagatcgg caatgaactg aatagcggaa tgctctggcc ttatggcaag tcttggggag
    20521 aaggcggggg agaatttgat cgattggctg cttttttgaa gtctggtatc caagctgttc
    20581 gagacactca acctaaaaca actccagtaa tgctccactt agctgatggt ggcgatacag
    20641 gagcatttac ttggtggttt gatgaaatca cgagtcgagg tgtgtcattt gatttaattg
    20701 gagtgtcgta ttatccgtat tggcacggtt caatgaatga acttcaaacc aatatgaatg
    20761 cgatcagcca acggtatggt aaagaagtga tcgtagttga aacagcttat gggcatacaa
    20821 cagcgaatgc cgatactatg ccaaatgctt ttggtgaagc cgaagcagct gcggggggat
    20881 atgaaccaac accgacagga caagcagagt atttgctgga tcttgccgat cggattcagg
    20941 cagtaccgaa taatcgcggg gcgggcttct tctattggga acctctttgg tacaacggaa
    21001 atgtgagttg ggcaacacaa gcaggtatga attatctcag tgttcaatcc actatgggaa
    21061 acgaatggga caaccaagcg atgtttgatt ttgctggaaa cgccttgcca agcttgcgtg
    21121 cgtttaaaca agcaggggcg caaataaatc tagttaaaaa tgcaagtttc gaaagcgatg
    21181 gaccaacgac cagtccttcc aattggcaaa cctggtttca acaaggaacc gctaagggta
    21241 cgatcaaaac ggaagcgggc actttttttg gggactacaa actaactttt tggcaggatc
    21301 aagcatacga ggcatcagct tatcaaaatg ttagtggcat aagcaaagga acttacacat
    21361 tgagtgccta tgtaatgtct gggggagagt ttgatactaa tcagttatat atcaaaaatt
    21421 atggtggagc ggaactaaat actgcggttg ttaaaacaag tgaatggaca aaaattacaa
    21481 tcaaagacat tccaattaca acaggtgttt gtgaaattgg ggtttatagc aaggcttcag
    21541 gaaatgcttg gacgaacgtt gattttgttt cattggtgaa acaatagact gcggatcgtt
    21601 tcgattcaag atcggaggtt cccttcagcc acatcgttag cgtagaaggc aggaatcgaa
    21661 cctgcacctc gcctttgcca aaaataacca gaaacgattt ccagtcattt ttaccaaaac
    21721 gagtaaagca ccagacttca tctacacttt gtattatgct aaagagagtt caacgatttc
    21781 gtaagaagca atgaaaaaat tgcaaaaagt tgtgcagata acataaagtt tgtttaagaa
    21841 agggtaacct tagaattccg tcgtgaatat gatttgagca cttagatgga tagaaaaaag
    21901 ttctcggaaa acagttcgtt tttcgagaac ttctttttat gataagaatg aaacaaatta
    21961 acttatatta tagtctcttt tggtagggaa ccatcttatt tttaatgaaa atcaagcatt
    22021 ttagctattg aagaatgttg atttataggg attccccctc catattatgt tggctcttta
    22081 ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg
    22141 ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc
    22201 aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta
    22261 agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg
    22321 ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc
    22381 gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg
    22441 accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc
    22501 acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc
    22561 tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc
    22621 gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag
    22681 ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg
    22741 ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat tgactttgat
    22801 gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg
    22861 tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag
    22921 aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc
    22981 aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc
    23041 ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg
    23101 acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc
    23161 aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa
    23221 gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg
    23281 atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga
    23341 accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc
    23401 taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc
    23461 gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg
    23521 gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata
    23581 ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt
    23641 caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat
    23701 gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta
    23761 tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt
    23821 tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat
    23881 aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc
    23941 atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga
    24001 cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat
    24061 caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa
    24121 caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct
    24181 ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac
    24241 gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac
    24301 gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg
    24361 cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca
    24421 agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga
    24481 tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat
    24541 cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac
    24601 ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat
    24661 tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata
    24721 atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat acataaaagg
    24781 atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc attactattc
    24841 tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa aaggcagaac
    24901 ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt atggttcacg
    24961 tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga tcttagatgt
    25021 gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc ggtttgttga
    25081 aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca
    25141 aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg aagaaaccta
    25201 tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac atgaaatttt
    25261 gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga tccagaagaa
    25321 acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt
    25381 cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag ctgaaaaata
    25441 gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca gttctttttt
    25501 tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat agtaggaaca
    25561 gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga aggctgccaa
    25621 gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct ctattctttt
    25681 cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat aggatgaagc
    25741 tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc tcacagattt
    25801 tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg tcgatcgtga
    25861 atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga cattcaaaga
    25921 ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg atcgaaggtg
    25981 tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct cgtcatagcc
    26041 aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac ggaatcttgc
    26101 tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt tatcaaaaaa
    26161 tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg cttatcgtaa
    26221 aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc catacgaaga
    26281 tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc aacggatatc
    26341 gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa catggagagt
    26401 tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa gatatcacgg
    26461 ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc gatgaattgg
    26521 gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag ctgtcagaat
    26581 acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga ttttatgctg
    26641 cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg
    26701 aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa ctcggcggta
    26761 tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt tagtaaacca
    26821 gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg aagacaagga
    26881 ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa acgagctgtc
    26941 gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc cagattatca
    27001 agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag aaatcacgaa
    27061 cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc ttgaaggaat
    27121 aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga ttttagcccc
    27181 cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc taggttacac
    27241 aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc tttcggatga
    27301 agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat caggaaactt
    27361 ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct
    27421 tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt
    27481 tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg caagacgtct
    27541 ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg cggttatctc
    27601 agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg tcgaggcaat
    27661 cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg ccttaggaag
    27721 ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca
    27781 agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg
    27841 gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct acacaatgga
    27901 gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc
    27961 tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc acgctgttgg
    28021 attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat
    28081 ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa atattgaaat
    28141 tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat aaaggaaaaa
    28201 agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc gaatgatcga
    28261 ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta tcgaagaagc
    28321 aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc tagcgaaaga
    28381 acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc taggagcaaa
    28441 cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg cagatgaagt
    28501 cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag tccaaaaaga
    28561 tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta ttattgaaac
    28621 atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag ctgcaggcgc
    28681 tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag aggatgtccg
    28741 tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg gtatccataa
    28801 cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa caagcgcagg
    28861 agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa ggagaagctc
    28921 atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga taaagcctat
    28981 gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg caagatttat
    29041 caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga gcgcacagct
    29101 tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt agcggggcac
    29161 acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga attctgttcc
    29221 cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac gaccgttcgt
    29281 gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat tcagtcagct
    29341 gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa aaagcaaaat
    29401 tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca tgcggcaaca
    29461 acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact gcagcattga
    29521 tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg gaaggactag
    29581 aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa tatttccaat
    29641 cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct ggattcaaaa
    29701 cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca acaaacaata
    29761 caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac gttgtatctg
    29821 caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct tatacgactg
    29881 aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat cgttttgatg
    29941 ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa atcaaagtat
    30001 tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt gctcaaggta
    30061 tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt aacggtgtat
    30121 tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt atcggtgttg
    30181 accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa aacttcacat
    30241 tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa aaatcagcgg
    30301 acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac ggtgttggtc
    30361 taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca aaagaaaaaa
    30421 tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac catttctata
    30481 gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa ttgttcattt
    30541 atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta atgtttgttt
    30601 caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag atagaaatcg
    30661 ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca gaatattacc
    30721 aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa ggcaggagaa
    30781 attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa cgtgctttcc
    30841 ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt aaagatcaca
    30901 agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt tatgcttgtt
    30961 gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg cgcagggatg
    31021 cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta tggattgtca
    31081 gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg ggtagaaatt
    31141 ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac agctgtattg
    31201 acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa agaaggcaag
    31261 tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga ccgctgtaca
    31321 gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac ctcacaacaa
    31381 ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa agaagcgaag
    31441 cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg tggattagaa
    31501 gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat cgctggaatc
    31561 gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa ggcagaaagc
    31621 ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa aatcactgaa
    31681 catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct agatatgaca
    31741 ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa aaacggcatg
    31801 ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata tgatgttcga
    31861 acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca aaaagcaatt
    31921 atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc aactcgtgga
    31981 ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag ggacaaatac
    32041 aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc ggatcgtatt
    32101 gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac atctgaaaat
    32161 gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga gttagaaaag
    32221 ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag tgcctgtact
    32281 ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg gttttcagcc
    32341 catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag ggataggtga
    32401 aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg tagctaactc
    32461 agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga tcgtcagtgt
    32521 ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt tatgcgtgat
    32581 cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc gagcattttt
    32641 tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat acgtaagcac
    32701 atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact cttccaatat
    32761 gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg gcggctctcg
    32821 cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc tgctgatgaa
    32881 gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg cttctgagta
    32941 tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg gcgctttggc
    33001 agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg ttcaaacgac
    33061 ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg gatcagcaat
    33121 cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac ttggcatgca
    33181 gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga ttatcttctt
    33241 tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag ggaaaaaaca
    33301 agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg aagggggcga
    33361 actataatgt cgactatcga attaattgcc ttgatcctta cttctacact ggtttattca
    33421 acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg gatcgtaaac
    33481 gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt caacttgacg
    33541 atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg tggcgtcgta
    33601 ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc tgaccatatt
    33661 atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt gatcaaagca
    33721 tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt ttctttccca
    33781 ggattagcag atattccggt cattggacca attttcttta aaaatacatt cttacctgct
    33841 tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac gaaattcggc
    33901 ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg tatcaatgta
    33961 tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat gggcggtgct
    34021 gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg acaagggttc
    34081 atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat gggtgcctca
    34141 ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc agttatttct
    34201 tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat tgtgctggtt
    34261 gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat caaatcaaaa
    34321 taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct cgcccttttt
    34381 gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa cgcgtacatc
    34441 tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag ttcggtgatg
    34501 ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt ccacatttag
    34561 agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg aaagatcatc
    34621 aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg acaggccact
    34681 gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat ggattccctg
    34741 atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt aacaaacctt
    34801 atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca ggcgatttaa
    34861 ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa atcattcctt
    34921 tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat gatccgtata
    34981 tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc acacgaacaa
    35041 gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat tctttgaaag
    35101 aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac ggacaaggaa
    35161 tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg ctgaaagtta
    35221 tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat gcattatacg
    35281 gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac ttacgaattc
    35341 cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc gatcacggaa
    35401 acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta ttggtttaca
    35461 gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat atttcagcaa
    35521 cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc ttgaaattat
    35581 taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga tgttacaaga
    35641 aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt tgattctagg
    35701 ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt cttatgatga
    35761 gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag tttatgggtc
    35821 tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg aaggtcattc
    35881 catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc attcagtcat
    35941 tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt tgatgctgat
    36001 taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg aagatgcaat
    36061 gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag caacagcaaa
    36121 agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg gctattctgg
    36181 accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag ccgatgcagt
    36241 agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa aagtgttagg
    36301 aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc atgaagaagt
    36361 tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag aaacattgac
    36421 attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa gaaggcgaga
    36481 tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt gcggagactt
    36541 ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt accggcaaat
    36601 ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca gccaccatct
    36661 atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta ggtacatgcg
    36721 gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt gcagcgacca
    36781 gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc agtgatttca
    36841 atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt catgtaggaa
    36901 atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc caattagctg
    36961 aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta ggcgcgaaat
    37021 accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc ggtgaagaaa
    37081 caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga ttggaaacag
    37141 cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata agaacatcca
    37201 agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa atgtaagctt
    37261 ttgaacaccg tttaccaaga ggttgtgaa
//
LOCUS       DACEKH010000019        36488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEKH010000019 DACEKH010000000
VERSION     DACEKH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.019"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(438..611)
                     /locus_tag="IW662_000875"
     CDS             complement(438..611)
                     /locus_tag="IW662_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HAQ0865718.1"
                     /translation="MNRYVHGTVQKKNRKINGKLLVNPNGYEWLRAKGSYPVPKYCNY
                     SETLMEWYQLIIT"
     gene            complement(954..1643)
                     /locus_tag="IW662_000876"
     CDS             complement(954..1643)
                     /locus_tag="IW662_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0865719.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFSHTRVGKNGKTFKMHKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYIGHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1706..2470)
                     /locus_tag="IW662_000877"
     CDS             complement(1706..2470)
                     /locus_tag="IW662_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ0865720.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIVKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(2498..3196)
                     /locus_tag="IW662_000878"
     CDS             complement(2498..3196)
                     /locus_tag="IW662_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ0865721.1"
                     /translation="MARTQKQKAAQTHVVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTIAANIAVVFAKSGQRVLLVDADLRKPVVYETFQLNNAS
                     GLSTALSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYRN"
     gene            complement(3208..3987)
                     /locus_tag="IW662_000879"
     CDS             complement(3208..3987)
                     /locus_tag="IW662_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ0865722.1"
                     /translation="MEETISLKEIFGTLRKHLATIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSENSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4003..4947)
                     /locus_tag="IW662_000880"
     CDS             complement(4003..4947)
                     /locus_tag="IW662_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0865723.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4993..5772)
                     /locus_tag="IW662_000881"
     CDS             complement(4993..5772)
                     /locus_tag="IW662_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865724.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKERVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNINY"
     gene            complement(6157..8232)
                     /locus_tag="IW662_000882"
     CDS             complement(6157..8232)
                     /locus_tag="IW662_000882"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ0865725.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDYEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTYHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDADRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(8234..9151)
                     /gene="glyQ"
                     /locus_tag="IW662_000883"
     CDS             complement(8234..9151)
                     /gene="glyQ"
                     /locus_tag="IW662_000883"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0865726.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(9537..10349)
                     /gene="recO"
                     /locus_tag="IW662_000884"
     CDS             complement(9537..10349)
                     /gene="recO"
                     /locus_tag="IW662_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ0865727.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKDMSEKDLPKKEE"
     gene            complement(10492..11391)
                     /locus_tag="IW662_000885"
     CDS             complement(10492..11391)
                     /locus_tag="IW662_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ0865728.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(11406..11807)
                     /locus_tag="IW662_000886"
     CDS             complement(11406..11807)
                     /locus_tag="IW662_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ0865729.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIVGLILFVPKIYHLLFN"
     gene            complement(11785..12261)
                     /gene="ybeY"
                     /locus_tag="IW662_000887"
     CDS             complement(11785..12261)
                     /gene="ybeY"
                     /locus_tag="IW662_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ0865730.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(12290..14482)
                     /locus_tag="IW662_000888"
     CDS             complement(12290..14482)
                     /locus_tag="IW662_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ0865731.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLFAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(14506..15477)
                     /locus_tag="IW662_000889"
     CDS             complement(14506..15477)
                     /locus_tag="IW662_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ0865732.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            16183..17562
                     /gene="brnQ"
                     /locus_tag="IW662_000890"
     CDS             16183..17562
                     /gene="brnQ"
                     /locus_tag="IW662_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0865733.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGTNWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(17718..18164)
                     /locus_tag="IW662_000891"
     CDS             complement(17718..18164)
                     /locus_tag="IW662_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ0865734.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(18191..18367)
                     /locus_tag="IW662_000892"
     CDS             complement(18191..18367)
                     /locus_tag="IW662_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ0865735.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(18529..18957)
                     /locus_tag="IW662_000893"
     CDS             complement(18529..18957)
                     /locus_tag="IW662_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0865736.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            19049..19909
                     /locus_tag="IW662_000894"
     CDS             19049..19909
                     /locus_tag="IW662_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ0865737.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(20004..20297)
                     /locus_tag="IW662_000895"
     CDS             complement(20004..20297)
                     /locus_tag="IW662_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0865738.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(20370..22097)
                     /locus_tag="IW662_000896"
     CDS             complement(20370..22097)
                     /locus_tag="IW662_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0865739.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            complement(22311..23237)
                     /gene="trxB"
                     /locus_tag="IW662_000897"
     CDS             complement(22311..23237)
                     /gene="trxB"
                     /locus_tag="IW662_000897"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ0865740.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(23382..24815)
                     /gene="ascB"
                     /locus_tag="IW662_000898"
     CDS             complement(23382..24815)
                     /gene="ascB"
                     /locus_tag="IW662_000898"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0865741.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(24815..26218)
                     /locus_tag="IW662_000899"
     CDS             complement(24815..26218)
                     /locus_tag="IW662_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0865742.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            26448..27350
                     /locus_tag="IW662_000900"
     CDS             26448..27350
                     /locus_tag="IW662_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0865743.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(27391..29277)
                     /locus_tag="IW662_000901"
     CDS             complement(27391..29277)
                     /locus_tag="IW662_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0865744.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIRAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILMMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGNLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKTSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFKSL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(29373..30224)
                     /locus_tag="IW662_000902"
     CDS             complement(29373..30224)
                     /locus_tag="IW662_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0865745.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSEQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGEKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(30681..32465)
                     /locus_tag="IW662_000903"
     CDS             complement(30681..32465)
                     /locus_tag="IW662_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865746.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQTITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(32465..34216)
                     /locus_tag="IW662_000904"
                     /pseudo
     CDS             complement(32465..34216)
                     /locus_tag="IW662_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(34358..34582)
                     /locus_tag="IW662_000905"
     CDS             complement(34358..34582)
                     /locus_tag="IW662_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ0865747.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            34833..36419
                     /locus_tag="IW662_000906"
     CDS             34833..36419
                     /locus_tag="IW662_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ0865748.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT        10109 a         7797 c         6430 g        12152 t
ORIGIN      
        1 tatcttaatt atctatccat ttcatcctac gcattcttgc gtaaatacaa ttcactctgt
       61 tgagctgata taaatcattg gtggtacaaa gataaaatga taactgtttc tatacctcca
      121 aaaattccag taaatatatc tatagatagt ttcaaaaaaa attgtcttag tttcataata
      181 tttaagatag tggtacatac tttataacaa cgtacttttc caaatattat tttccgttat
      241 gaacatccaa tatattacaa agtttaaaag tttagatgaa ctataatctt catttctgca
      301 cagtggataa taaattctta tagataaaaa atagcctctt ctaaagtcac aaatacctta
      361 ctcccacatt ttaagtaaac actctgcagc attctctttg ttataaaatg atagatattc
      421 ctcaatttta tctcttatta tgttattatc agttgatacc attccattaa tgtttcagaa
      481 taattacaat attttgggac cggataactc ccttttgctc gtaaccactc atatccgtta
      541 ggattcacta aaagtttacc gtttattttt cgattctttt tttgaaccgt cccatgaaca
      601 taccgattca acaagctaaa ttataaaaat tggtgtgctg tcattatttt ttggaaaatt
      661 tattaatggc tatcaatata cttttgtatc atactaaata gctcgtcagt tcctatatcg
      721 gatataccaa tattaaagca tttagatcat tatatttaag agtggtatca tcccaaaatt
      781 tcgaatattt tcttttatat aagaataatg atactgaatt gccccattca cctttctttt
      841 tttagatttt ctacaagtga ttcaaaccct ccataattac agttattact tttgaaacga
      901 taatatgaac atgtgtaatc ttttttatta acaatccatc attctatccc taactaatat
      961 gctccattgg gcttgacaat aacataaaac gtttttaaaa tcaacctcaa gtcgtacatt
     1021 aaacttcgat gaccgatata ctctaaatcc aactcaacca ttttatggaa actaacatta
     1081 tttctatcac taacttgcca caaacctgaa caaccaggtt ttacatacaa gcgttgaaga
     1141 tcatatctcg tgtactcagc tacttccctc tccaaaggtg gccgtggacc aattagactc
     1201 atatcgcctt ttaaaacatt ccatagctgt ggcaattcat caatgctttt ggcacggata
     1261 attttaccta ttttagtgac acgcggatct tctttcatct tgaacattgc accttctatt
     1321 tcattttgat caagtaattc ttccaatttt tcttcagcat ctaaacacat cgaacggaat
     1381 ttatgcattt tgaatgtttt tccattttta ccaacacgtg tatggctaaa aaatattgga
     1441 cttcctggct cttcttttct gattcgataa gctacataga acatcaaagg agaagctaaa
     1501 atcaaaccaa taatgcttcc tacaatatca atcattcttt taaaaaataa atagatatat
     1561 cgtttttcaa ctttttcttt atccaaccaa acttgattag gatgtacttc actaatacga
     1621 gataataaat cttcactttc catactttca gtccctttca ctatcttcac tctctcctca
     1681 tttgttttta tctacgaata aagaactaaa ataaaccaaa cttcttcttt tttacctctt
     1741 catagattgg ataactgatc tcatccccgt tgatcagatc ctttgcgact tgtttcatct
     1801 gtactacttt ttccttacca aagtccttga caatctgctc atagcactct tttaagtaaa
     1861 aggtgcgctt agagaccccg tgtgcatcag aagcagccat ctgcacgaga ttatgtttga
     1921 ccattgtttt ggctgttttc tggatgtctt tgccaaatac accaacataa cttggcgcag
     1981 taatttgggc caaacatccc atatctagga acggaagcaa atgatttggg tcttctcgaa
     2041 aatgcgcgtt gcgttctgga tggacgatca ctggtgtctt tcctagttct aacaactcaa
     2101 aaaaaagcca ttctgcatat aacggcactt ccagtgtcgg aaattcgatc agaagatatg
     2161 tatcatctaa atctgtaaat aaaatttcat ctcggtggat gtcttcgatg agcgttccgg
     2221 taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc tctaattcgg
     2281 cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat tttccattat
     2341 tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt tcagccattg
     2401 caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata tggcaatgta
     2461 gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttctgtag taataaccag
     2521 catctttgct atgttctgct ccgttgtata caacacctaa gactcgtgct tgtaccatct
     2581 ctagcagttc ttttgcttta gtcaaggatt ctttacgtga tgtattttca cgtacgacca
     2641 ataaagtgcc atccgttttt gaagacatga tctgtgcatc tgttacggct actactggtg
     2701 gcatgtcgaa gatcactaca tcaaatagtt gtcttgcttc tgctaaaatc tgatccattc
     2761 ttggcgagct taataactct gatggatttg gtggtttagg tccacttggc agaatcgaca
     2821 aattctcgac tggtgttcgt tggatctcat ctgctacact tcctgatgaa ctaagtgctg
     2881 tacttaatcc actggcattg ttcagctgga atgtctcata gacgacgggt ttacgtaaat
     2941 ctgcatcgac tagtagaact cgttgaccgg atttagcaaa aacgactgca atgtttgctg
     3001 caatggtgga ttttccttct ccaggacctg atgaagtcac gacaatcgtt ttgatttgct
     3061 gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt tcggcgattg
     3121 gtgaagatgg gtttgttaat gtgatcaaac tgactacatg agtttgtgct gctttttgtt
     3181 tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc gtgatggagc
     3241 tgttttatcg tttgtagctt ttggtcgtgc aggagctgat ccttttttac ggataccagc
     3301 gttcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc ctaatgtatc
     3361 cgtcacatat ttttcatctt tcactgtccg atcaagtaat tcgaataaga atgccaaacc
     3421 tacgcctacc atcattccta atactaagcc aatcactaga ttcaatttat tatttggcga
     3481 aacaggtgtc gtattggcaa ctgcacctga gatgatcgag attttatcga cattcatcac
     3541 gtcttttgcg ttctcttgga aaacttgtga agttgtatta gcgatctgtt gagcagtcac
     3601 tgcattcgtg cttattgcct ggatcgagaa catttgtgag ttttctgact gattgacgga
     3661 gatcgcatct ttgatttgtc cagcggtcat tttcacattg tcttctgttt ctagacgatc
     3721 tttgacttca tttaatacta gatcactaac gatcatatct ttatacgtat tgatcatttg
     3781 caggttcgta ttgacatcat tgacattcgc tgtgtctgat tgcggcagtg tcacgatcaa
     3841 ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc ctgagatggc
     3901 caagccagca aacattgaga tgatgattgt tgccaagtgt ttgcgtaatg ttccaaaaat
     3961 ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat tctctagatt
     4021 catttgatct tttaattctt ttttcacacg atcaagttct tgatcgctga ctttttgata
     4081 ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttga tcgtaccaaa
     4141 tgcctcccga taatctaaag cgattttttt catttcatca aagctcatgt ctgttttcat
     4201 attcgattct agagcattca aaacggattg atagttgctg acaccgttta gactcaatac
     4261 ttttttcgct acaccttcta cgatctttct ttgacgctct tgtctaccat agtcaccatt
     4321 tggatcttca taacgcatac gagaataaga taatgcctga tccccgttca atgtgatttt
     4381 accgattgta aagtcatagc catcttgtga gaaagtgagg tcattattga cttcgatacc
     4441 accaactgca tcaacaagtt cttttagccc tttcatattg atagagacat agtgatcgat
     4501 tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc cgaatgcgta
     4561 agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg tatctcgagg
     4621 aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca tcatcgtatc
     4681 tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca atagaacaga
     4741 gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg ttcgttccac
     4801 tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg ctcctactcc
     4861 tacaattgct aatactaata cgataagaac tgctaaaata atcagaaata tttttttccc
     4921 tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt gcttgaatca
     4981 tcgctttaac tgtcaatagt ttatattgct atagcccatt tgctgagcaa cagtttgtgc
     5041 ttttgttgcg tatgctacgc ttattttttc attccggatc tgctctattt gactgagcgc
     5101 cgtcaaatat tgatcgtaag tagcggtggc agtcacttga ccatccttta acatcgtatc
     5161 gaataactga tctagttgcg tgacttgcgc gatctgggta tcagcgaatc ctagatattg
     5221 cataacaacc gttttccaag gactatcttt aaaaaagctc atattttcac gtacacgagc
     5281 gacatctgca cttgtcaccc tttctttgat gatgacatcg tctacagctt gctgccagtt
     5341 tgatacattt ttagtaaata aatcgttgac agctgtctgg atcttcagtt ttgattctgc
     5401 actatctaac tgtgtcacga ttgctttctt ctcatcttgg attgcaacag cttctttagg
     5461 caattctgat tctttgatgt cgtaatcatc tgctgaaagt ttgacagagg atagcttatt
     5521 tgacaaactt gtgattgttt cttctgttat ttcagggttt agaaaaattt tttcttttgt
     5581 actatacagc ttgtctactt cttttgataa agaagtcaag gtgtcttttt gattggtgat
     5641 ggcagattct gcataattga tccgttgctg atgttgccgc tgattattga tatggtatcc
     5701 aaacaaacag atcgcaatag cacctgcacc taccgccaca gcagtcaatg acttgacgaa
     5761 tggcttttcc atagattcgc tcctaagtat tgatgataga taaattaaga tataattcac
     5821 ttacataaaa atgacagttt tttatttaaa aaaacataat taaactaata tattcacaga
     5881 aatatttgta aacaagtttt ccctatataa tcataccaca aactgctttc ttgactaggg
     5941 aaatttacga tttaaaagta aataaaacat taataagata acaattaatc gattttactt
     6001 ttatttcctt aattttcata ggtgttttta aaagttacac aaaaaataaa ataacaaaaa
     6061 aaattaacat tgaattaata atagttattt ataacaaata aaccagagaa atagcctatt
     6121 ttgtgcctgt tctctggttt atttacatat tagaaattat tttgtgatga tttgatctaa
     6181 acttgctagt gacaaagcca tggcagcaat ttgacttaat tgcgttaatc ggttcttacg
     6241 tactgctggt tcttctgcca tgaccatcgt ctgatcaaaa taattttcga tcagtgggcg
     6301 aaggcttaca agctgatcat aattttcgtt gatcgtttgg tcagcaaagc ttgttttcat
     6361 tgcctctact gctttgtgca gttctttctc cgcttcgttt tcgaacagtt cagggtctac
     6421 ttctgattgt ttttcaagct ccgctttttt cgctaagttc acgacacgtg tcaaagcttc
     6481 catcgatggt ttgaagtctt catctgcaag atgctgcttg aacaattgtg ctgctgcaaa
     6541 tacttttgtc agatcttttt gttctgcatt caaaacagct tcgatgacat catgacgaat
     6601 attcttcgtt gtcaataatt ggcgcagacg gcctttgata aagtcgatca cctgttgttg
     6661 tcccgctgac aagccaatgc caaaacgatc cgcatcttta ctgataactt cactaatcgt
     6721 ttcttgcaga acagataaag gaaaggccca ttttttcgat tcgatgattc gaacgacacc
     6781 atatgcttga cggcgcaaag catatggatc gtttgagcca cttgggatca tcccaactgc
     6841 aaagaaagaa aagatactat ctaatttatc agcaatcgct aaaacagaac ctactgtagt
     6901 ctcaggtaga tctccttcac tggatatagg catgtaatgt tcacggattg ctgttgctac
     6961 ggctggtttc tcgcctttaa gcagagcata tttttctccc atgatccctt gtagttcagg
     7021 gaattctcct accatgttgg ttactagatc aaatttatag atttcagcag cgcgttgcag
     7081 atccatcaat tcttcctcag atagtcctac ttgctcgcct atgatgcggg cgatgacttg
     7141 tacccgttgc attttctcgt agatgctacc gattttttca tggtaagtaa cagtttttaa
     7201 tttttcaacg tattggtcga ttgaagcttt cttgtcttca ttataaaaga attccgcatc
     7261 ttctaaacga gcagtcaata ctttttgatt gcctcgaacc acattttcca attttacccg
     7321 atcaccatta cgagcagaaa taaaatgagg aagcagcata ccttgatcat tccgtacctc
     7381 gaaataacgt tgatgttctt tcatagaagt gactagtact tcttcaggaa cagaaagata
     7441 tttttcttcg tagtcgccag aaaagacagt tggatattcg actaaattgt tgacttcttc
     7501 tagtaaatct tcgtctagat caataaccca gtgatttttt tctgctaatt cgtttgcttg
     7561 tgtaacgatc atttcttgtc ttttttgcgg agacgcaatg acgaattgtt cattcaattt
     7621 ttcttcatat tcttttgcat gcgcaaatgt cacatcttcg cctaagaaac gatgacctcg
     7681 agaggctctg cctgttttga catccaagat ttcgaatggg attacttcat catctaacaa
     7741 tgccacgatc cagtgaatag ggcggatata ttcaaaatcg tattctgccc aatgcatcgt
     7801 tacagggaaa gtcaggcttt taatgacatc tgctaaacct gtcaatactt ctttggattc
     7861 cttaccatga gtgtatttcg ttacatagac atattccaca ccttttaatt ctctaaaagt
     7921 gatagcgtct gtcgtcagac cttgtccgcg gacaaatcct tccgctgctt tcgtccagtt
     7981 tccatctgcg tccaaagcaa tttttttggc tggtcctttg acttcttctt cactgtccgc
     8041 ttgtcgttct ggaatatttg ttactttgat tgctaaacgg cgcggtgtgg aaaatgtttc
     8101 gatcgtatca taagtcaatt ggttttcatc taaaaatttt gctgtttttt cttctaattg
     8161 tttcatgctt ggtgtgacaa catgggcagg catttcttcc agcccgattt caagtaataa
     8221 gtcttttgcc atcttattca gcctccttca cggtttctgg atttgctgtg tttttattca
     8281 acaaagggaa tcctaatttt tcgcgttctg ccacaaagat tttggctaca gcacgagcca
     8341 tgttgcggat acgggaaaga taaccggcac gctctgttac ggatactgca ccgcgagcat
     8401 ctaataaatt gaatgtatgg ctgcatttca aaatataatc atacgctgga tgaacaagat
     8461 tttcatcgat acagcgttta gcttcttttt caaaacgatc aaagttgctt agtagcaagt
     8521 cttgatcgct tacttcaaaa gaatatttgg aatgttcata ttctggttgt ttgaagatct
     8581 cgccatattt cacgccatca gcccattcta aatcatagac actttcaact tcttggatat
     8641 aagaagcaag acgttctagt ccataagtga tctcagaagt gactggttta caaggcagtc
     8701 caccaacttg ctggaagtac gtgaattgtg tgatttccat tccgtcaagc catacttccc
     8761 aacccacacc ggcacagccc atggatgggt tctcccagtt gtcttcaaca aaacggatgt
     8821 cgtgttctaa tggatcgatt cctaaaaggc gcaagctttc taaataaagc tcttggatat
     8881 tctcaggtga aggtttcatc acgacttgga attgatgatg ctgatacaaa cggtttgggt
     8941 tttcaccata acgtccatca gctggtcggc gagatggttc tacgtaagca gcattccatg
     9001 gttctggtcc gattgcccgt aaaaaggtat atgggctcat cgtacctgct cctttttctg
     9061 tgtcatacgc ttgcatcagc atgcagccgt ttgatgacca aaatttttgt aatgttaaaa
     9121 tcatatcttg gacggtcaat tttttactca tctgttttgc ccctttcaat tttaaagcgt
     9181 tgctgtcaaa taagaataca ggaaagaaaa cgacaaaaaa tccctatgtc tgattacaga
     9241 catagggacg aataattcgc ggttccaccc tacttccgaa taaagattcg gcagcttcat
     9301 ttatcgtact cgcaagtgcc tcttcaaaaa tcgtttgggt ccggctccca ctctcccgaa
     9361 ctcgctaaac cacgtgtttt ttactgatct tggtcaatgt acgtattcat tttgcagttt
     9421 tcttgtttct ataatagatc ctctcactga acgatctatt tgatctacgg ttaataatag
     9481 acggaactcc ctgtgtttgt caaatggttt tgattttttg ttgtatgttt catttactat
     9541 tcttcttttt taggtagatc tttttcagac atgtctttct ctggcgtctt taatacgtgt
     9601 gcccaagact tcatttgatc gatgaacttt ttgcttttca aatgaatacc gacatattca
     9661 tcatacagct ggtcgatgag ttcacggatt gcttgtttgg tttcttcttt cagatggatt
     9721 ccactgattt tatcataaga aatagctgaa aacatccgaa cgaaataaac agctcgagga
     9781 tctgcatgat aacggtgatc gtccatcgac caatgtcttt cgcatatcac accaccatat
     9841 ttggaagaat agtcaaattt tccttttgtt tccccacata cgacgcagtg tgtccagatt
     9901 ggtgcaattc cgaagcgttg cagtatttga acttcaaaga tattcgtgat gatttctgca
     9961 tcatctcctt gatccatccg ctgcagagct tgttgcgtaa attggaataa atgcgggtca
    10021 tattgatgat cttcgattgc tgcatctact agatttagca aataggtacc ataaccgctc
    10081 aaaaagatat cttcttggat gttccgaaac gcatggacgt ctttgacact gcttaaaaac
    10141 gacaaacctt cacttcgaaa attaccgata tagactgctt cggtaaaggg gagcaaagcc
    10201 ggagctaatg gctgattttg ccgatggatt cctttgatat aaaacatcat tttgccagaa
    10261 gattccgtaa agattttgac taatttatct ttttctttgt aattacggct agataaaatc
    10321 aagcctttcg attctgcaag ctggctcata ttctccctcc taaaaaataa atctggaaaa
    10381 gcagattctg ccgatccaga ttatttatgt ttcgatcttc cgccgttttt ctcaacgtgc
    10441 ggatttttat ccagtttatt tctatactta acctataaga agttcagaat atcagtattc
    10501 atcttttcgg tagccgaagt cttgcagata gacttttttg tctcgccagt ctttttggac
    10561 tttgacccaa agttctagaa atactttgtc tcctaaaaga ttttcgatat cctttcttgc
    10621 tttcgttcct acttgcttca gcatttttcc gcctttgccg ataatgattc ctttttggct
    10681 atctctttcg acgatgatcg tggcctggat atggatttta tcattctcgt ttcgcttcat
    10741 cgaatcaaca acgactgcga cagaatgagg cacttcgtct cgtgttaaaa acaagacttt
    10801 ctcacggatc aattcagaaa cgatgaagta ttcaggatga tcagtcactt gatcatctgg
    10861 aaaatattgt gggccctctg gcatttcact gactaataca tccatcaaac gttcgacgtt
    10921 gtttccttct gtagcagaaa ttggaatcac ttgtgcaaag ggcatctgcg ttgaataatc
    10981 ttcgatgatt cccaacaact cgtctggatg gattttatcg attttattga taatcagata
    11041 aacaggagaa tgcacgtttt ttagacgttc aataataaaa tcgtctcccc ggccgcgttt
    11101 ttgatcagcg ctgatcataa ataagaccgc gtccacttct ttcaacgcac tgtaagctgc
    11161 ttctaccata aaatctccta aacgatgctt tggtttatgg attcctggtg tgtcaataaa
    11221 aatcaactga gcttctggtg tagtatatac tccttggatt ttatttctag ttgtctgcgc
    11281 cttatcactc atgatggcaa ttttttgacc cacgattcga ttcaataatg tggattttcc
    11341 tacattcggg cggccgacga ttgcgacaaa ccctgattta tgttctggca tatacgttct
    11401 cctttttaat taaacaacaa atgatatatt ttagggacaa ataaaatcaa cccaacgatg
    11461 acggcaaaac aagcggtcac taaaactgct ccagctgcca tatctttgat ttttttccca
    11521 atcggatgaa aatgaaaatc cgtaaacatg tctacgacat tttcaaaaac agtattgatg
    11581 atctctacta tccatacgag aaaaacagct aaaagcagcc ataaccattc tatccgatca
    11641 agttggaaaa tcacgcccat aaacaaagcg aggattccga acgcaacatg tttcctcatg
    11701 ttgcgttctt cctcaaaaac agttttgact ccttggacgg caaattccac ggacatcagg
    11761 aaatgtttgt ttttctctac gtttttatct tttaagtcca taggcatcta agatctcttt
    11821 ctgtaaacca aacatttctt tctcatcctc tggtgtcata tggtcatacc cgttgatatg
    11881 caagaatcca tgaaccgcta aaaagccaag ttcccgatca tacgaatggc cgtaagcttc
    11941 tgcttgctct gcagcacgtt cagtagagat catgatgtct cccagtacac gaggcaatgg
    12001 attctctcca tcttcaaaaa tcactgggat ttcgtcttca ccctcttctt ccaaggcaaa
    12061 actgatgacg tctgtcggtg catcttttcc acggtaatca cgattgatta cttggatcgc
    12121 tgcgttgtcc atgaaagtaa cagacatttc tgtttcttct ggcaactcta aatatgatgc
    12181 tgcaaattgc aacaagtcgt cgatttcttt cattttctct tctgagactt ggtttgtttc
    12241 atccatgaag gtaatatcca ttatacatcg cttctttcta ttcttcttgc tattcttctt
    12301 gttcttcttt tatcttctca acttccgatt tcattttagg atatttgatc cgagaatgga
    12361 aggtactgct taaaccgctg atcaatgatt tttctacttt acggatttct tttaacgtca
    12421 atccactgtc atccaattgt ccatcggaaa tacgctcttc tataagatta tggacaaaac
    12481 tttcgatttt ttcgcttgtt ggatggtcca ttgcccgtac tgccgcttca caactgtcag
    12541 ctatattgac gattcctgct tcacggcttt gaggttttgg tccaggataa cggaattgct
    12601 cttccgtgac ttccgggttg cgttcctttg ccttaacgta gaaataacgc atcagcgtgg
    12661 ttccatgatg ctgtctgcaa atatcgatca ccatttgcgg catattataa tcttccaata
    12721 ttttggcacc atctgtcaca tgtccaaaga taatatgttt actgtcttct ggcaataaga
    12781 aattgtgcgg attttccgct cctgaaggca agttctcaac gaaaaagctg gcgtgtttga
    12841 tcttgccgat atcatgataa tagcaagcta ctcgagttag taaagaacgt ccaccgattt
    12901 ctgctacagc attagcactt agattggcta ccatcatgct atgatgatac gtacctggtg
    12961 cttcttctaa caactgtttc aataaagggt gattaggatt gctgagttca tttaatacga
    13021 tcacactatc atcattgacc atcaattcaa tataggggtg cagacctatc gtcagcaaga
    13081 atgagaacaa tgcacctgtg aatccgcaga ctagcgttaa ccaagtcgtt gcatccccaa
    13141 agctcattcc ttggtagata atcaaaatga catccatcat cacagggaaa ataatgaccc
    13201 acatagcagc gaataaccct tgttctgaga tccgtttgcg tctgacgatc gttgccatga
    13261 gaccagaaaa tacataggac atcaaaataa ctgtcagaaa gttcgtgcca atcgaatcat
    13321 aaaaaatgaa aagtgcggat actgcttgga aaataccagc catgatccct gctcttcgat
    13381 tcaagaaaaa gcttaaaaca agtggagcaa aagccgcggg gtaaaacagc ggaatataag
    13441 cagcttgttc tgtttggaag agttggaaaa atttcattag tactacgctg actgacattg
    13501 ctgtcacgta gaaaagtacg tattttgttc tttctcctgc attttgaaat tgaagtgaat
    13561 tataaatcag tacaccgatt tgaagcaaaa cagctaaaac catggcaatg agtgggaaaa
    13621 tcgaagtcgt ttgattcgtc aatcctagta attccaattt tttcatcgct gaagcatcaa
    13681 tttgatttcc ttcgcgaaca atgatttctc cttggaagat catcactggt tgtacagctt
    13741 ctcgtgcaga ttgttttaat tcttctgtct ttttctcatt caatacatca ttgactacaa
    13801 tgccttgatc cactaagtag cggatcattt gttgctgctc tgttgtgaca ttcaaatact
    13861 gaatctgatc ttctgcttct tgtttaaaag cttccaattc gctttcacga acttgtttac
    13921 tcatctgttc gtcaatgagc ttcaaacttt catccctaac cgtgtgaatt tccgtttcag
    13981 tcatttcaaa tatcgtcgaa taaaaattgt tcggcagttt ttggtaaaac gcaacgtttt
    14041 ctgcattcac gttttcaaaa tttttcttca gtgtagcaat ccgctcttct gtagtaggtt
    14101 tcgggatatt ttcatcttcc ttcgccttat ctaccttgtc attataagat ttgttgatgg
    14161 tatcattcgt cttatcgatc aattcgaata attgcttgat ccgattgttc tggtcatctg
    14221 ccagatcttt ttgataagta tattctggag tcactgcttc tgctgctagt tttcttcttt
    14281 gttcggtttc ttccgtgttc tcgatcgttt tattcgcacg gatactctct tctgctactt
    14341 ggccttctct aaaatcattg gccttttgtt tgacactact aaatgtaacg ccgcaaacaa
    14401 tgataaaaaa caagaacaga aggaaaggca atgctgcctt tccgagtttt tcttgtagtt
    14461 tctgcagtgt tttattcaac atgtgcttat cctcctaagc aaagcttatt cttgatgaag
    14521 atccgccttt tcataagctt tgatgatttc ggcaactaca gggtgccgaa caacgtcact
    14581 tgcttcaaaa ttaacaaaat cgattttttt gatttgcttt agtgttcgtt ctgcatgaac
    14641 aagaccactt gtcgtccctc gaggcaagtc tatctgactt gtatccccat tgacaatcat
    14701 ttttgattga taaccgagac gggtcaagaa cattttcatc tgagcgactg tcgtgttttg
    14761 tgcttcgtcc aagataacga atgcatcatc caatgtccgc ccccgcatgt aagctaatgg
    14821 tgcgatttcg attacgcctc gttccatcaa tcggttcgta tgatccaatc caaaaatttg
    14881 gtataaagca tcataaacag gccgcagata aggatccact ttttctttca gatcccctgg
    14941 tagaaagcct aggttttccc ctgcctctac agcgggccgc gtcaaaatga ttttttgcac
    15001 ttcgcctttt tttaaggcag caattgctaa tactacggca agaaacgttt tccctgttcc
    15061 ggctggccct acaccaaata cgatgtcatg tttggctacc gattcaacat atttcttttg
    15121 accgctgttt tttacccgta taggttttcc attacggtct ttgacgatct cttcctcata
    15181 catctcaaca aaataatcta atgtaccttt ttgccccatt ttcaatgccg tcacaacatc
    15241 gggcgtactt acgtggattc ctcgtttgat caattcctgt aatgtccgaa tgactgaggc
    15301 agccaaggaa atctgggatt gttcaccaat gatctgaatg atttctccac gtgtatggat
    15361 ggttgtttcc gttgtttcct cgatcagttt tatatgcttg tcgtgtgcac ctaaaagcat
    15421 gctgacatca tcagcagctg tcaatctaat ctctaaagaa ctagactctt ccgtcaaaaa
    15481 tagacatccc ctttttttcg attcattctt tcctatttta ccatactcag aagaaactat
    15541 atagcaaata agaaaaggtt gtaaaagagt cgtttaacca tacgtaccaa gaaacgttaa
    15601 aaaatagccg tttccattcg ctaaaggtct cgttttgata aaaagaatct gagaatgtaa
    15661 gttatatgaa agtggagaac aaaatattcc gtttcattca gcaggtattc tctaggtatt
    15721 tttcttgttt caaaaaaaga aaacaaaata gttaaaatat tcttgcattt caaaaaaaac
    15781 aggtgtatta ttacgtcaac aaataaacat acagcaaagc gtcgaggaaa acagtaggct
    15841 attcaacgat ttattcccag tgagtcatgt aagtgagaaa tgacaataaa tcgaatagac
    15901 gaacatcatt tccgagtcaa tgtcgaaaac gtagatcatt gtggctgcct ccattatcag
    15961 gctagcggat aactttatca aaaagcgtcc gtgaatgaag aagctttttt aagcgaaaaa
    16021 aggtggtacc gttctagaat ctagagccct tttcccaagt ggaaaagggc tttttttata
    16081 ttttaaggtg gtggttttca tggaatctga tattaatggc attactaatt gctgcaacgt
    16141 ttatatcgtc aatcaaaatg ataattattg gaggaaaaat ttatggaaga aacaaaaact
    16201 tattcatgga aacaactatt gatggtcagt tcacttatct tcgggatgtt cttcgggtca
    16261 ggtaatctaa tctttcctgt ccacttaggt caaatggctg gcacaaactg ggtcagtgct
    16321 ggcgtaggat ttgccatctc aggcgctctt ttcccattac tagctatctt agctgtagta
    16381 gtgacaaaaa gtgatggtct gtatgactta gctaaacctg tcggaaaatg gtatgctgcc
    16441 ttgttcttaa tccttgttca tttgacgatc ggaccatttt tcggtacacc acggacagct
    16501 gctactgctt ttgaaatggc tgctaaacca tttttgcctg aaaaatataa ttcattaggt
    16561 atgtttttat tctctgctct tttcttttta cttgcttatt tccttactgt ccatcccaca
    16621 cggttggtca aatacgtcgg aaagtatcta aactccttgt ttcttgcatt attagcaatc
    16681 atttttgtcg tttctttatt ccacccaatg ggaaatctaa atcaatctgc tgcagatact
    16741 taccaaacaa acgctttaac aaatggtgtc ctagaaggtt ataacacggt tgatgcagta
    16801 gctttgcttg ccttaagtgt cacttttgtc catgcagtcc gtgaccttgg tttcaaagat
    16861 aaaaaagtca cagaattaac agcaaaagct ggtagcctga gcgttgcgct agaagtcatg
    16921 atctattttg gacttgttct tctcggtgcg atgagtttga ataagatgtc tttatcagaa
    16981 aatggcggaa ctgcactttc tcagattact aatttttatt taggtaactt cggcgctagc
    17041 ttcttaggag tgatggtcac attaggtgtc ttcactaccg ctatgggact cgttgtttct
    17101 tttgcacaag atttccataa actgttccct aaagtcagtt atatgacatg gctgcggtta
    17161 acgactttcg tctcttttgt cgttgcaaat gctggtttgg acaatatcat ccaatggtct
    17221 ttaccagtat tgatgctttt gtacccattg tcgcttgcgt tgatcttgct ttccttaaca
    17281 gcaaaatttt tccaaaagac accatttgtc tatcaagtaa cgatgttgtt tgctgctgtt
    17341 cccgccgtat tggatatgct ggcaaattcg cctgctttag tgagtcaaca aagagttgta
    17401 gcaagcatgc tcgaatttta tcatcatcac gttccttttg ctgcattagg acttggctgg
    17461 atggtcccaa cgttacttgg ttacgctggc agcttactct tctattacgc ctatcgccta
    17521 tcagggtaca aacaagaagc aaatgaacta ccagaagaat aaccagatca aacacaattt
    17581 aacataagaa atattaaaca ggaaaaaacc agctagatga aagaatccta ttctttcgtc
    17641 tagctatttt ttagatttct tttttacgag cacaaaaaaa ctgcggacaa tgattgtccg
    17701 cagtttaaat gataaaatta agattgtaat agttctttca caatggcatt tacttggttt
    17761 ccatctgctt ttcctttgac tttaggcatg atcgcaccca tgactttgcc aaattctttc
    17821 attgatgaag caccagtttg tgtgattgct tcttgaacaa gttgtcgaat ttcttcgtcg
    17881 gaaagttgtt ccggcatgta tttttcgact atcacaattt cactttttac tttttcagct
    17941 aaatcgtcac gtcctgcttc ttcaaactca tgcaaagaat cacgtcgctg tttcatttcg
    18001 cgagacaaca ccgttaattc ttcttcttct gtcaagtcgt gaccttcttt aatctgctcg
    18061 ttttgtatag atgacttgat cattcggatg acttgtaaag attccttatc tttcgctttc
    18121 atcgctgttt tcatgtcatc gtttaatcta cttagtagtg acacactacc aactccaatt
    18181 catcacagaa ctagaatttt ttgcgttttc tagctgcttc agattttttc ttgcgtctta
    18241 cacttggttt ttcgtagaat tcacgtttac gagattcttg taaagtaccc gcttttgaca
    18301 cggaacgttt gaagcgacga agagcatcgt caagtgattc gtttttgcga acgactgttt
    18361 ttgacatgtt aattccctcc ctccgagctt aaaaagtaac cgtaattgtt attgaaatat
    18421 atctcaaaaa acaagactgt cctgtaaaca ttatatctaa tgctgttttg accgtcaagg
    18481 aaatcttgct tttttttaaa attatcaaat gttattttat ttaaatcctt attgacaatt
    18541 ttcacatctt ccgaaaatct cgaatctatg cccttcgatc gtacatccac cgagctgttc
    18601 ttcgaaataa ttcattggac acatatggat ttctttcgtt gttccgcaca ccgtacagat
    18661 aaagtgatgg tgatggccga tttccccttg acagcaatga aaacgaaact tcatctctcc
    18721 atttaaatcg gtttcttcca gtaaatcgat ttctgcaaaa tcatggagat ttcgataaat
    18781 cgtatcgtaa cttactcctt tgaattcctg attcatatag tcaaaaactt ctttagcgga
    18841 tacgtagcga ttgcgcttca ctaaatatgt taacaatgct tctctttttt tagtatattt
    18901 aaaaccgtta tttttcaatg tttctagtga ttgttcgatc aatgtctgtt ttttcatgac
    18961 ttattcctcc aaatcaaaat gattacgaag tacacattct agagtatggt ataataaaca
    19021 taaaaaaaat gcaagtgagg ttttatccat gtcttccgat tctacagaaa tcgttacttt
    19081 ttttgtcatc tccgactctg ctggagaaac tgcgacaaaa ttagcgcaag ccactatggc
    19141 ccaatatcct agcgttgagt tcaatttatt ccgcagaaca tttgtgacag atgagaaaac
    19201 attgatgcag gcactgcaag atgcactaga agaaaaagct cttgtcttac acacattgat
    19261 caatcaagaa ttgatcgaga tgacgcgttc tttttgtgaa gaacatcaat tatttagttt
    19321 tgatgtgatg actccgccga tatcagaaat cgagcgctta actggtatca agcccattcg
    19381 gcaacctggc gcccttcatt tattgaatga gaactatttc aaacggatta aagcaatgga
    19441 atttgctgta aaatacgatg acgggaaaga tcctcgtggg tttctagaag cagatgtcgt
    19501 tttgctaggc gtatcccgaa cttccaaaac gcctttgagc ttgtttttag ctaacaaaaa
    19561 tttaaaagtt gccaatcttc ctttgatccc acaagcacat attcccaaac agctttggga
    19621 gattgatcct aaaaaaatcg tgggattgac gaataaccca gatattttaa acaatatccg
    19681 taaagaacgg atgcgctctt acggattaaa tcctgatact gcttattctg atatcgaaaa
    19741 aatacgagct gaactcgatt ttgcaaatga tctttatgaa aaactgggct gcgtagtaat
    19801 caatgtcgct tctttatcta ttgaagaaac agcttcgctt attttgaatg ctttagacct
    19861 agaagatcat agttattatg gaactgaaac caccgaagaa acagactaac agtttttctt
    19921 gtctttttct taaaaaaaac tggactgtgt cagcgaagaa gattcgttga cacagtccag
    19981 tttgtctcta tttgcaccta tcgttattga tgttccacat gtttgagtgt ctggttcaaa
    20041 atatccagta cgtgagaatc atcaagacta tacaaaatcg cttttccctc acgatgggac
    20101 ttgacgacat ggttttctct taataaacgc agttgatgcg atacagcaga ttgttccatt
    20161 tccactgctt gggtgattgc tgtcacattt tgttccccgt ctttcagata cagcagaatt
    20221 ttgagacgag tggggtcact caacattttg aatagttggc tgactttctc tacttttgtt
    20281 ttttgcgtgt ctggcaatcc attcacctcc aacttgaaaa aagactgact tctaaagttt
    20341 accacttttt ccatcagtct caagtttttt tattcttctg tcatcttttg gataatcgct
    20401 tcataagcgg ctactttttc agctgcgtct tctgaagagt ttccttttac accgatgtaa
    20461 aatttgattt tcggctcagt tcctgaagga cggatagcga tccagccatt ttcttccagc
    20521 gtatacttca atacatcaga tggcggtgtc gttagttttt ctacgtttcc ttcacggtct
    20581 gttctcgtca actctttgaa gtcttctgtt tggatgactt ttgctccggc aaattctgtt
    20641 ggtgcttctt cacggaattt ttccatcaaa gcagagattt tcgcagaacc ttcttgtccg
    20701 ctcattgtca cagaaatcgt tttttctgcg tagtagccat aggtttcaaa aatcgtttgc
    20761 aagccgtcat acaacgtttt gccttgtttt ttatagtaag ctgctacttc tgctaataag
    20821 agcaatgctt ggatcgcgtc tttgtcgcga acgaaaggtt taactaaata accgtaactt
    20881 tcttcgaatc cgaacataaa agtatgggaa tgatcttctt caaattgttg aattttttca
    20941 gcaatgaatt tgaatcctgt caatacattg aacatggtag ccccatagtg ttccgcaatt
    21001 gctgtaggta attcgctaga aacgatcgat ttcaataccg ctgcgttttc aggtaaagtg
    21061 cctcgcgatt ggtgtgcttc caaaatatat tgagcaaaca gtgctccgat ttggtttccg
    21121 gtcaatactt cataggagcc atttggcaaa cgaacggctg cacctaaacg gtctgcatca
    21181 ggatctgttg caatcagcaa atcagcgcct tctttctcgc caagtcgtat cgcatactca
    21241 aaagcagagt gttcttctgg atttggtgat ttgactgtag tgaaatctgg gtctgcgaca
    21301 gcttgttctg gaactagtac gaattgttcg aatccagctt gtttcaacgc acgttcgccc
    21361 aacatttttc ctgttccgtg aagtggtgtg tacacaagtt tcaaatcttt ccccattgtt
    21421 tccaccaatt catgatcaat cgttactgct ttgatttctt ctagataagc ggcatccact
    21481 tcttctccga tgatcgtgat caatccgcta tgttcagctt cttcgtcaga taatacttca
    21541 acttttaacg gattgctgac ttcacggaca tatttcgtca atgcatctgc gtctgcagga
    21601 ggcatttgtc ccccatcttc gccgtagact ttatatccgt tgtaagctgc tggattgtgg
    21661 gatgcagtta ccatgattcc cgcaaacgtc ttcaaatgac ggactgcaaa agaaagctct
    21721 ggtgttggac gtagactttc gaatacataa gatgggatat tgtgatgtgc taatgttttg
    21781 gctgcttcca tggcaaattc tggtgattga tggcgtgagt cataagcgat agcgacacct
    21841 cggcgttttg tttcttcgcc ttgtgtatcc atgaaacgtg caagtccttc tgttgcttgt
    21901 cttaccgtat agatgttcat tcgattgatc ccaggcccta agattccccg catgcctgca
    21961 gtaccgaatt ccaacggtgc ataaaaagca tcttcaagtt tttcaggatc ttcacccaga
    22021 tcatttaatt gtttttttaa gttttcttct aaattttctt catgtaccca ttgttcatac
    22081 acttgttccc aagacatgaa aataacacct ctttctgaaa tatggttctt aataagtata
    22141 gcattcactt attagatata aaagtatgag gaccaaaaaa cgcttaaatc aagaaaggat
    22201 tcttttatca agcacggttg caagtattcg cataaatcgg attcagtaga tttcacgcga
    22261 aaaaaaagag taaaagaaca attgaaaccg ttcttccact ctttttaaac ttatgcagtt
    22321 gtttcttcta aagattccaa gtatttatac acttgttgtc cagcgatccc accatcacca
    22381 acagcagtag tgatttgtcg caaatctttt tcacgaacat ctccgatggc aaaaacgcct
    22441 ggtatattcg tcttcatttc gttgtctgtt ggaatccagc cagcttcgtt cgtcaaccct
    22501 gcttctctaa atggtgcagt taatggatcc agtccaacat agatgaatac tccgtctgct
    22561 gccaaatcaa actcattgcc ggttttgaca ttacgtgctt tcacacctgt cactaccatt
    22621 tcattcccaa cgatttcgtc aacgactgta tcccacataa aggaaatttt ttcattcgca
    22681 aatgcacggt cttggatgat cttttgtgca cgtaattcat ctctacggtg gacgatgact
    22741 acttcagaag caaattgtgt caaatagatg gcttcttcta ctgctgagtc tcctcctccg
    22801 atcactacta gacgtttgtt acggaaaaac gcaccatcac aaactgcaca gtaagaaacg
    22861 ccacgtccag caaattcttc ctcgccaggt acgcctaatt tacggtggat acagcctgtt
    22921 gcgataatga ctgtttttcc ttcgtaagtt ttatcttcac aaataacttc tttgtggctg
    22981 ccgtgatcct tgatatcttg cacgattcca taagcatttt ctgtcccaaa tcttgaaaca
    23041 ccttcataca tcttataagc tagatcagga cccatgattg aatcgaaacc aggatagttt
    23101 tcgatttctg ccgtattgtt catttggccg ccaggggcgc cttgttcgat catcaatacg
    23161 gataagtttg aacgtgaagc atataaagca gcagtcatcc cagcaggacc tgcgccaata
    23221 acgattacat catacatatg tagattctcc tttacttaat caagtaagca tactttacag
    23281 ccaatgcgaa aaaaagtcta ttgttctgct tgcgtttctc gaatagcttt attatactcg
    23341 aaaaatccgc tgaaaaacaa tagactttta taaatcattg atcaatcatt cgaaagattt
    23401 tctccatttg aatcaattac ttttttatac cagttgaatg atttcttctt cgaacgggct
    23461 aatgtgccat ttccctcgtt gtcacgatcg acataaatga atccgtacct tttcttcatc
    23521 tcacctgtac cagctgatac taaatcaata catccccaag tcgtatagcc taatagatcg
    23581 actccatcta attctactgc atctttcatt gcttggatat gtgctgccag atagtcgatt
    23641 cgataatcat cttccacata accattctca tcgggcgtat cgactgcacc taagccgttt
    23701 tctacaataa atagtggttt ttgataacga tcataaatat cattcatcgt tattctcaag
    23761 cctaatggat caatttgcca accccattca cttgcttcca gatatggatt tttgacagaa
    23821 gcaaagatat ttcctgcagt ctgttccaag agttcgttgt cagtagtcga aactcgagaa
    23881 gaataataag aaaaggagat aaaatcaaca gtatgttctt tcagcaatgc ggcatcccca
    23941 ggttccattt cgatctgaat gccatttcgt tccatttctt tcaatgcata agcgggatat
    24001 tctcctcgtg actggacatc aatgaagaaa taattttctc gatctgattt tctagaagcc
    24061 caaacatctt caggattgca agtgtatgga taatatgctc ctgctgccag catacagcct
    24121 actttgttct ccggatctac ttcatgagca atcttcgttg cgatggcact tgcaaccaac
    24181 tcatgatgtg ccgcttgata tttgatttgt tcttcatttt ctccttcttc aaaatacagc
    24241 cctgcgccca tgaatggtgc atgaaggatc atattgatct cattgaatgt caaccaatat
    24301 ttgacaagtc ctttatagcg attgaagatg actcggcaca gattttcata aaaaccaacg
    24361 agttctctgg aacgccacgc accatattct ttgatcaaat gcatcggaca atcaaaatgt
    24421 gtaatcgtca caagcggctc gatcccgtac ttttgacatt ctttaaagac atcctcataa
    24481 aatttcaacc cagattcatt tggctctttt tcatcgccca ttggaaaaat acgactccaa
    24541 gcaatggaca tccgataagt cttgaacccc atttctgcaa ataaagcaat atcttcctta
    24601 taatgatgat acatatcgat cgcaattttt gctggataaa aatgattttc atcaaattca
    24661 aacattttct tttttccagt aatgacagga aaacggtctt ctccgattgg cacaacgtct
    24721 acgttcgcta acccacgtcc gccttcatca taaccgccct cgcattgatt ggctgctgta
    24781 gctccgcccc ataaaaaatc ttttctgaat cccatcatac ttcctccatt acatgtgtat
    24841 tgcttgataa ttctcttgcc agttcatctt ggacttcgat tccttctgct tttaaccggc
    24901 tgattgtttc cttgaattgc ggtaaatact gctgatgtgc aataaacaac tcatccatta
    24961 tccgttttgc tgtctttcct gatcgtataa gcggattgat cgtaaacgct tgcaaagctg
    25021 ttccataatc accagtcacg gctgcttcga tcgtcagcaa ttccatattc ttcattactt
    25081 gcaaccagcc tctttcagca gcaggaaggg aaccaaacgc tacatttcgt gcgccttgtg
    25141 cgccaacata ggcagtgact tctactacac aatctgccgg cagatcagga acagctccat
    25201 cattctttgt agataccacg atctgtgttt ctttatttcc atagatcgaa gcaatcgttt
    25261 cacaggcagc atctgaataa taagcaccac ctcgtttttg caattgttca ggtttatgat
    25321 ccaattctgg atctttgtat aattcaaaca gctctgcttc tgtttctttc acttgctgcg
    25381 ctcttgtacc aatcgtttga tattcttcta aagcatgttc cagcatttct tcttcacggt
    25441 agtaatagcg gtggtagcca caaggaatca tgttcatttg ttttaattgt tctttgaaaa
    25501 acgggatatc aaagatattt ttcggcaatc cattgtcttc atcgtataaa cgatcaatca
    25561 attctgccgt gcggtcattt cctttattat ctgctacttt gtgccaatgg aagtgattta
    25621 gaccggcaaa cttgtagatc agctcttctt ttttcttgcc aagcattgtt ggttctgtca
    25681 tcatcgccat tactggcaca ttgcataatc cgatcacttt atcccatttg ccatatctga
    25741 taactgcttc tgtcaccatc ccgcttgggt tcgcgaaatt gatcagccaa gcatttggac
    25801 ataaacgctt catgtcttca acgattgcca aaaccactgg aacggtacga aaagctttga
    25861 atatcccgcc agcaccattt gtttcttgcc caatcatccc ataataacta gggatccgct
    25921 catctttgat tcgtgcatct aattgaccga cacgaaactg agtcgtaaca aaatccgcat
    25981 ctttcaatgc ttcttcccga tccagagttg catagatttt gacttcatac ggagaagcat
    26041 cccacatacg ctgagacatc tgactgacga tctcaagctt ttcttttcct tcttctacat
    26101 ctacgagcca gatttctcgt atcggtaatt cctcatatct tttgataaat ccttccatca
    26161 actcgggggt gtaactgctg cctccaccaa tcgtagcgat tttgattcct tttgccatgt
    26221 tctcactcct ttacttttta cacttttatt atcttttttg taaacaaaac aaacaataga
    26281 aattcgtttc tttccagtaa catttacaaa agttagtgtt ttcttctgga tttttgtaaa
    26341 tatcttgtca aaaaatgtct ttattaagct atataacata aatccgttta caaatcattt
    26401 tcaaaaaagt tataatttac gtaaaaaaac aagaagaagg tatctacatg ttattacgtg
    26461 aaaaaatcaa agaaatctcc ttttctcctt cagaaaatga agtgattcag tttctcttaa
    26521 gcgattctcc agcacttggc gaaatgacca tccaagagat tgctgagcga tgttatgtcc
    26581 acccgtccac actcatccgt gtagcaaaaa aaatgggttt tgatggctgg gtcagttttc
    26641 gtaatgcttt tttagctgaa acagcctatc ttgaagggaa ttttcaagac gtagatgcga
    26701 atcttccttt ttccaccaca gacggtatta tgacgatcgc aaataaaatg gcttctttag
    26761 aacagatgac gattcaagat acactttctc tcattcatca tgaccaactg caaaaagcca
    26821 aacaactaat gcttcaagca aaaaaaatcg tgattttcgc cagtaactcg aatactttga
    26881 tttctcaaga tttcatgctg aaaatgaagc gcatcaaaat ggatgtcacg attgtcacca
    26941 cttttggtga atcgttctat gaagcgtaca attgcacgag tgatatgtgc gggattctga
    27001 tttcttatac gggtgaaaac aatgtgatcc ttcgtaccgc agaaatattg aaacaaaatc
    27061 atgtgccaat tttagctttg accagtatcg gtgattcaac gctatcttca atgagtacag
    27121 ccgctttgcg acttacgaca cgcgaacgac tgtattcgaa aattggtaat tttacgatca
    27181 atacatcgat ttgttatctt ctagacgtgt tatatagttg tgtttttgca gaaaattatc
    27241 aacagaattt agtccatcta atttctgtag gacaaaaatt tgataaacgc cctatcagct
    27301 cagccatcat gaaagagcct ttcaattttg aaaataaaga agtggactaa aaagaataat
    27361 taagcctagt ccattctttc acctaaccgt tcaggtcagt gcggtgataa gtgcatcgtc
    27421 tggcccgacc gtctcccctt gattttccaa aatatccaaa taatccgtgt aattggtgac
    27481 gatcacaggt gtttctaaac tgtatccttc ctcttcgatt ttcttcttgt cgaagcggag
    27541 caacagatcg ccttgtttca ctctttctcc ttgctgcacc aagcttttaa aatattttcc
    27601 ttctaactga acagtatcca atccgacatg aatcagcaat tccagacctg cttcagaaac
    27661 cattccgatt gcatgtttcg tcgggaacag ggtcatgact gttccgtcaa aaggggcacg
    27721 tacttctcct ttttcagggt agatcaagat tcctcgacct aatgttcctt gtgcaaaagc
    27781 tgcatccttt gctgcattca gtggagcgat ttgtccttgt atcggtgttt ttacaatttc
    27841 tttacctgat gttttctgtc tatcatctgt ttcatctgct tcctccttcc aaaagaaaaa
    27901 agtcagcgca aaggatacca ccatcgcaat ggcgatccca atcaatacat taatcacatc
    27961 tgttaaatta ccatctggtc taatatagtt gggaattgta aagatgccta gtccaccttg
    28021 attccaagaa gttaaattca ttaacccact atacgcgccc cctaacgcac caccgacaca
    28081 ggtaaaaata aatatttttt tacgtggcaa tgtaaaccca taaatagctg gctctgtaat
    28141 ccccacaata ccagaaacaa tggctggtat tgcaattgct ttgtcttttg gatttctaag
    28201 cttaaagtag agtgcagtaa gtgctgcagt ttgtccaaaa gacactactg caacaggtgt
    28261 catgatattt tgccagccat tcacagaaaa ttgcatcaat cccattggta cgattgccca
    28321 atggagacca aacatcatta gaatttgcca gaagaagcct acgattgctc cataaataac
    28381 tggcgaaagt gctaaaagtg tttcaaatcc agccattaaa atatcagttg cgaatgtgaa
    28441 tgctggtcca acgagtaaaa aacctaaggg cattgtaata agaacggtga agaatggaac
    28501 gaagaaattc gcgatcatct caggtactaa ctttttaatt ggtttctgaa tttggctagc
    28561 taacaacaca ataaagataa tgggaatgac agaagaagcg tagttacgcg caatccaggg
    28621 aatgccgaat actgtttgat aaataggtgc ttcaaaaata gtgccagaaa atatggtcgt
    28681 cagtggtgct tctgctgcac cactcaaagc ttccagcgac aagctaggat tcattaatgc
    28741 tgccccaagc aacatcccta cgaattcatt tactttaaat ttacgggcag ctgttaaacc
    28801 gataataaca ggcataaatt ggaatacaga atcaccgata ttatcaaaca taacaaatgt
    28861 gccagaaccg cggtcaaatg cgcctaagaa gacaagtaat gctgtcagtc cacgaatcat
    28921 cccaccagca gctagaatag caaggaatgg ctggaaacag cccgatattc catcaataag
    28981 catatcaaac ggtctcattt tttctgttga aacagcctca tcaaaagtaa ggtttcctgc
    29041 tcttataacc tcttcaaaca cgactggcac atgattcccg atgacaactt ggtactgtcc
    29101 tcccgctcgc attaccgtca caacaccgtc catatttttc aagatctcat catttgcttt
    29161 ttcttcgttt tttaatttga aacgaagcct tgtgatacaa tgtgtcaaac cattgatatt
    29221 ttccttcccg ccgacatttt ctacaatttt gctcgctaat tcttgatatt ttcccatttc
    29281 tattccctcc aatttattag tggaaggttt agccaactga atgtcactac ccttatttca
    29341 tgcgttcttg ttaatttatc ctcctttttt atttatttac aacgattctc atgcactaaa
    29401 cgcgcagtat ggatcgttaa atacattttt tcgtccgtgg acatctggta gttgaatatc
    29461 tcggctaaaa agtctgctat tttctcaacg caaccaaacg tatccttata ttttttttga
    29521 ataacttggt acaattcctc agaaacttct cccacttctg atttttctcc atctaggacg
    29581 cgtgtcagaa aaaaacgtag gtgtgtgacg aatcgaaaaa aatggactga atcttcatca
    29641 aatgtaatct tatagtaata gcggacgata tttagaatat cctgtattgt tgtagtaaac
    29701 aggtaggtat tgtcttgttg gctttgaatg ccttgtgcat tgactaaatg caatgcgata
    29761 aatccggctt catcttctga taacttgatg tggtatttat ccgagatttt cttcactgtt
    29821 ttttccccga tggcatactc tttaggaaat agttgtttga tatcatacag caatacattt
    29881 ggtacaaata ttcccatttt gtgacgttcg atagccgtat acaaatgatc cgtcaatgca
    29941 atatacactg aatcattcaa ctggacttgt agagcctctt ttgtatattc aatgatttca
    30001 caagaaattt ctaagtactc aagaggaatc gcttgtgcca attcttggaa ctgttctgaa
    30061 agtccgtcat ttgtcaactg aaattttttg tcgatgcatg agtcaaaaat cgggtcaccc
    30121 actttctttt tgaatgccaa accttttccc atcacgatca tttcctgatt tctttcatct
    30181 ctggttatta cgacgttgtt attcagtact ttctcaatca gcatgtttta ctcctttcca
    30241 tgatatatag ggctgtatcc aaaacaaaaa gaaaaaccca aaaagccgtc catggaacca
    30301 tgggagactc atcaggttta gcttgcatca ggcaatcact atccaagttg tatcgttttc
    30361 tttatattat cgaaagcaag cgcttttgtc aacgctttct tagtggctat taggcacttc
    30421 tgactagctg tcgaatcttt tttaaatctg gtgactacga cactaccgct cacagatgaa
    30481 gaatctggga agctctgttt tattttcact ctagcgctta ggtcaaaatg ctactatgct
    30541 actgtcccaa cttctaatat acggtgttcc agtatcagct tatcggtgat aagccaaatt
    30601 tatgcaaaaa tctggaaagc cttttttaca atttttttct tatcacctaa agctgaacgg
    30661 agccctgtca caaccacttt ttacacacca ctgctttgca gtttgtacat atctgcatac
    30721 aaaccatttt gcgctagaag tgtttcatga ttcccgcgtt ctactatatg acctttgtct
    30781 aaaactaaaa tcaattctgc atcccgaatc gtggacagcc gatgggcaat cgcaatcgtt
    30841 gtccgtcctt gtcgcatttt tgtcaatcct tcttggatca atccttctgt ttcagtatcg
    30901 atatttgctg tcgcttcatc caatactagt attttaggat ctgtcacgat tgttcgcgca
    30961 aaagtgatca gctgtcgttg tccgctagaa tagctggcac cgtgttcgat cacttttgag
    31021 tgatatcctt ttggtaattc atcgatgaac ttgtttgctt gaacaaactc agctgcatgg
    31081 cgaatctgtt cgtctgtgat agttggattc atcatccgga tatttcctgc gatatctcca
    31141 taaaacaaga aggcatcttg caagaccaat cccattttct tacgtaactc ttccattgga
    31201 tattctcgaa tgtcatgatc atcaatcaag atctggcctt cataaaattc gtaaaaccgc
    31261 atcaatacgt tgatgatcga gctttttccg cttccggtgt gccccactaa tgcaacggtc
    31321 tctcccggat tagcgacaaa agagatattg ttcaatacat tgtgctctcc atcataagaa
    31381 aagcttacat tgcgaaactc gatcttacct gaagtaatcg tttgatctgc tccaggattt
    31441 tgttgcggtg catattcttc tgtatccatg attttcaaaa tacgactacc ggcaaccatt
    31501 ccatctgtaa aggtgctcaa aaagtccatc atttgagaca ttggattaaa aaatccctgg
    31561 acataggtcg taaatgcata gatcagtcca acttctacgg gagaatgcag cgcatcgatc
    31621 ccaaacaaag tcaacgccaa cgcaatcgcc aatgcatata gaaaattgat gatcggactt
    31681 aacagaatcg aattcgtttt gatcatcgca tagcgcgtgt ctaaatactc gttgttcact
    31741 tgttcaaatt ctttagctaa acgagcttct tggcgaaact gctggataat acgcatgccg
    31801 gaaatcgatt cattcaattt cgtgttcagc tgacttaatt tttcccgcat attccggtaa
    31861 atacgagaac tgaatttctg gtaataccag atgacgattc ctaaaatcgg caagaacacc
    31921 aaagtccaca aggcaatttc tccattgatt tgaaacatgg caatgaagga agaaacgact
    31981 gcaaagatcc cagtcaatac catcaaaaag acataccaaa attcaaatag cgtttctgtg
    32041 tcatttgtca ctcgagaaac ggtcgatcct gccggtgtct gatcaaaata acgcatacct
    32101 agtgtatgca gcttttcaaa taatcttacc cgaacatact gataggtctt caatgaagcc
    32161 atggaataca aataccattg gaaaaaccaa atgatacttt tgactatgac tccaaacaag
    32221 tacataccag caaaaaacaa aataacttgt gtcgtagccg actgtttagc cagatgatca
    32281 tccataaaag tttggatgat ccttggcaat aggacattga tcacagacag agcaaacgca
    32341 aataagatag caacgatgaa cgttcttcta aaaggtttgg cgaacttcat cagtcgtttg
    32401 atgatcttct tctgttcttt aagtggaatc gattttgtcc attccgattg ataattttca
    32461 tccatcaggc ctcgcctcct tctatcttcg cttctaattg ctgtttctcc cacattcgtt
    32521 cataccagcc gcctaatgca agcagctcat gatgggttcc tcgttcaatg attttccctt
    32581 gatcgacaac aatgatttct tttgcatgca tcacgctgct taaacggtga gctgcaatga
    32641 tggttgtttt ctcttttctt atttctttta aattatgcaa gatagcttct tctgttttgg
    32701 catctactgc agaaagtgca tcatctaaga tcagcaattc aggatttttg atcaatgctc
    32761 gagcaatcga aatcctttgt ttctgtccgc cagataaaga gactccacgt tctccaacta
    32821 aggtatcata accttttggc aattccttga tttcattgtt gataaatgct aaagaagccg
    32881 catgctctac ttcttcttct gaaagttttg gattagaaaa acggatatta tctcgaatgg
    32941 tcatcgaaaa caagaagtga tcttgaggca catagccgat tgatcctaat aaagcattca
    33001 atgaatattc tttgatcgaa cgtccgccaa attggatttt tccttgataa tcatcgtatt
    33061 cacgcatcag taatttgatg atcgtcgttt ttcccgcacc agttttccca acaataccta
    33121 atgtttctcc ttcttggatc acaaatttga tttgttctaa tgctggtgat tcttctttcg
    33181 gatagttaaa agaggaaata gctacattca gctcgccttt tgccggggtc tgcactgcac
    33241 ccagttgttc cacgatatgc gtcttttcat gcaaaagttc attgacgcga tcataagaag
    33301 catttcctct ttccaatacg ttgaacaatc gaccaatcgc aaacatcggc caaacaagca
    33361 taccgatata gctaataaat gagaccatct gcccgatcga gatcgtgcct tccaagacat
    33421 actttccacc taaaataatt gttgcaacat aagacagacc gataatcaag gtaatgaatg
    33481 gatcaaataa ggagtctaaa aaattgactc gtttgttttt aataattgca tcttcaatct
    33541 tttcttcaaa atccgcgaga tcttcttttt cttatccaaa tgttttaatg actttgattc
    33601 cagtaatact ttcttgtgtc ttatcattga tactagaaaa agcttcttgc gaatcacgaa
    33661 aagcatcgtg gagttttgac cccagcactc gtgaagtaac ggctagtaaa ggcagaggaa
    33721 tcagcgcaat cagtgttaag cgccaatcaa taaacaaaat catcgcaatg atcgttgttc
    33781 cgcccgtgat cactgaatct gcaaaggtca agataccagc tcctgctaca ttttggattg
    33841 cattcaaatc attcgttgca tgagccatca aatctccagt ccgatatttt tggtaaaaga
    33901 gattgtccat ttctgtaaaa tgtttgaata gctgctgccg aagttctttc tccaaccgag
    33961 ctgcacttcc ccaaatattc gtccgccaga tataacgaaa aagatattgt aagagagctg
    34021 caaggatcaa taataagatc caccaggaaa tgatctttat tgataaattt ttatcggcaa
    34081 tctcatcaat gatgatccca atcacctttg gaggaactaa ctgtaccaag gccactgcga
    34141 atagggagaa aatcccaatt aaataatgtt tcttttcctg tcgaaaaaac caacctaatt
    34201 ttctaaaaat cgacatttta ctcctccttt gtctttatca agagcatagg attgtctgtg
    34261 cttgtctttt ttgctgaata gtctgaatag tctaacaaat tacgaaataa aaaacggact
    34321 gaaaaaatca gcccgcaaaa agtctttggg tcagaaccta ttttttccct tggttcttca
    34381 tttgctgcat catctggcga attttcttct cagatggttt ttgccccatt gacatcatca
    34441 ttgatcgcaa catgtcctca ttaacgggag gatttttttc taaatagtct ttcatatatt
    34501 tacgcgctag gaaaaagcca cctactgcgc cgattagcaa agcaataata gcaataagta
    34561 cgacaatacc tgttgatacc atataaatct ctcctttcta tttccgaatt gctctcaatg
    34621 atttatttta ctaaagttta ttgaaaaaag aaaggcgtat tctttagttt catgcaaaaa
    34681 atagtggttt ttttagagaa gaacaaaatt tctttcatta accagcaaga cacgccacga
    34741 aaaaaaatta ggcataccga atatttttct ttaatttttt ctttctgagg tgtagaatgt
    34801 aatcgtagat aaataggaaa ggtggttttt tcttgaataa tcaatccaac aaacaatttc
    34861 ataagcatcg acttattgaa catacaaatg gtttatcttt agctgaagta aatggaacag
    34921 tggatgtacc caagggaaaa agttttttta aaacacttct tgcttactct ggaccaggtg
    34981 cattagtagc agtgggttat atggatccag gtaactggtc cacttcgatc actggtggac
    35041 aaaatttcca atatttattg atgtcagtta ttttgatgtc tagtctgatt gctatgcttt
    35101 tacaatatat ggcagcaaaa ttaggaatcg tgacccaaat ggatttggcc caagcaatcc
    35161 gagcacggac aagtaagaca ttgggtgtca ttttatggat cttgactgaa ttagccatta
    35221 tggcaacaga catcgcagaa gtcattggtg cagcaatcgc cctctacctg ctttttgata
    35281 ttccattagt gattgccgta tttatcactg tgttagatgt tttcgtcctc ttattgctga
    35341 caaaagtcgg gtttcgtaaa atcgaagctt tggttgtctg cttgattttc gtcattttat
    35401 tcgttttcgt ctatcaagtt ggcttgtcta accctgattg gcacagtttg tttgccggct
    35461 ttatccctaa cagtcataca ttctcagact caccggtcat cggcggtgaa actccgctga
    35521 ctggagcttt agggattatt ggtgcaacag tcatgccgca taacttatat cttcattcag
    35581 cagtttctca aactcggaag atcgatcacc aagatgaaga agatgtagca caagcggtac
    35641 gcttttctac ttgggattct aatatccagt tgtcaatggc ttttctagtc aatgcactgc
    35701 tattgatcat gggtgtcgct gtattcaaaa ccggttctgt ccaagatcct tccttttttg
    35761 gtctgtatga agctttatct gatccatcag ctatgagcaa tggtatcttg attgctgtcg
    35821 caaaatctgg tatcttgtcc actttatttg ctgtagcact tcttgcttct ggccagaact
    35881 ctactattac tggtacgttg accggtcaag tcatcatgga aggcttcatc catatgcgta
    35941 tcccgatttg gctaagaaga ctagtcacac gattgctttc tgtgattcct gtattgattt
    36001 gtgttttgtt tacaagtaca aaaggaacga tcgaagaaca taccgctttg aataacttga
    36061 tgaatgaatc ccaagttttt ctggcttttg cattgccatt ctcgatgatt cctttactga
    36121 tgatgaccaa tagccaagcg gagatgggga gccgattcaa aaattcattt gttgtaaaac
    36181 tacttggctg gttctctgtg attagtctga cttacttgaa cttacgagga ttaccaggtc
    36241 aaatcgaaag tttcttcggc gagcaagcca cctcagctca actagcattg gctgatcaaa
    36301 tcgcttatgt aatcattgtt cttgtccttc tcctgttggc atggacgatt ggtgatttat
    36361 ataaaggaaa caaaaaatat gaacagcgtt tgcaagaaac gatagaagaa atcgaataga
    36421 aactgattct tttacacaaa ggaggttgtg acagaagtgg gcagcttcaa gaaataagaa
    36481 ggaattta
//
LOCUS       DACEKH010000020        34574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEKH010000020 DACEKH010000000
VERSION     DACEKH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.020"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(178..2070)
                     /locus_tag="IW662_000907"
     CDS             complement(178..2070)
                     /locus_tag="IW662_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865749.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2067..3794)
                     /locus_tag="IW662_000908"
     CDS             complement(2067..3794)
                     /locus_tag="IW662_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865750.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3810..4460)
                     /locus_tag="IW662_000909"
     CDS             complement(3810..4460)
                     /locus_tag="IW662_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0865751.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4664..4960
                     /locus_tag="IW662_000910"
                     /pseudo
     CDS             4664..4960
                     /locus_tag="IW662_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            5132..5752
                     /locus_tag="IW662_000911"
     CDS             5132..5752
                     /locus_tag="IW662_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ0865752.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5844..7010)
                     /locus_tag="IW662_000912"
     CDS             complement(5844..7010)
                     /locus_tag="IW662_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0865753.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7022..7375)
                     /locus_tag="IW662_000913"
     CDS             complement(7022..7375)
                     /locus_tag="IW662_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0865754.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7503..8525
                     /locus_tag="IW662_000914"
     CDS             7503..8525
                     /locus_tag="IW662_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ0865755.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8964..9398)
                     /locus_tag="IW662_000915"
     CDS             complement(8964..9398)
                     /locus_tag="IW662_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865756.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9508..11367)
                     /locus_tag="IW662_000916"
     CDS             complement(9508..11367)
                     /locus_tag="IW662_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0865757.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11485..12645)
                     /locus_tag="IW662_000917"
     CDS             complement(11485..12645)
                     /locus_tag="IW662_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ0865758.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12952..14376)
                     /gene="araA"
                     /locus_tag="IW662_000918"
     CDS             complement(12952..14376)
                     /gene="araA"
                     /locus_tag="IW662_000918"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ0865759.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14392..15090)
                     /locus_tag="IW662_000919"
     CDS             complement(14392..15090)
                     /locus_tag="IW662_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0865760.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15047..16708)
                     /locus_tag="IW662_000920"
     CDS             complement(15047..16708)
                     /locus_tag="IW662_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ0865761.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16926..18326
                     /locus_tag="IW662_000921"
     CDS             16926..18326
                     /locus_tag="IW662_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ0865762.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18578..19657)
                     /locus_tag="IW662_000922"
     CDS             complement(18578..19657)
                     /locus_tag="IW662_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0865763.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19743..20702)
                     /locus_tag="IW662_000923"
     CDS             complement(19743..20702)
                     /locus_tag="IW662_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ0865764.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20714..22237)
                     /locus_tag="IW662_000924"
     CDS             complement(20714..22237)
                     /locus_tag="IW662_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0865765.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22247..22810)
                     /locus_tag="IW662_000925"
     CDS             complement(22247..22810)
                     /locus_tag="IW662_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0865766.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22816..23679)
                     /locus_tag="IW662_000926"
     CDS             complement(22816..23679)
                     /locus_tag="IW662_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0865767.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23693..24589)
                     /locus_tag="IW662_000927"
     CDS             complement(23693..24589)
                     /locus_tag="IW662_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0865768.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24764..26035)
                     /locus_tag="IW662_000928"
     CDS             complement(24764..26035)
                     /locus_tag="IW662_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0865769.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26025..27542)
                     /locus_tag="IW662_000929"
     CDS             complement(26025..27542)
                     /locus_tag="IW662_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0865770.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27740..28630
                     /locus_tag="IW662_000930"
     CDS             27740..28630
                     /locus_tag="IW662_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ0865771.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28703..29527)
                     /gene="nadE"
                     /locus_tag="IW662_000931"
     CDS             complement(28703..29527)
                     /gene="nadE"
                     /locus_tag="IW662_000931"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ0865772.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29539..31011)
                     /locus_tag="IW662_000932"
     CDS             complement(29539..31011)
                     /locus_tag="IW662_000932"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ0865773.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31648..32010)
                     /locus_tag="IW662_000933"
     CDS             complement(31648..32010)
                     /locus_tag="IW662_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0865774.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32233..32985
                     /locus_tag="IW662_000934"
     CDS             32233..32985
                     /locus_tag="IW662_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0865775.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33125..34048)
                     /locus_tag="IW662_000935"
     CDS             complement(33125..34048)
                     /locus_tag="IW662_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0865776.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34063..34335)
                     /locus_tag="IW662_000936"
     CDS             complement(34063..34335)
                     /locus_tag="IW662_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865777.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10219 a         7196 c         5938 g        11221 t
ORIGIN      
        1 aaatcagcgg tttgacctga gtattagaga tacaaaacgg aaaaatagct tctcatattt
       61 tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccgtttat tcggaaaaag
      121 ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg tactattcta
      181 tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc cgtctttttc
      241 catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca caataatgtt
      301 gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc ggttttccaa
      361 taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac tagatgttgc
      421 ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg tgattagctg
      481 acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat atccttctgg
      541 caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga tttgttcgtc
      601 tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa acagccaagt
      661 atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat cacgggtatc
      721 tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa gaaggttgat
      781 cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac caggtttgac
      841 atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt gaacattttc
      901 aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt cattggacat
      961 ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg attggatagt
     1021 gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat attgcaagaa
     1081 cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc ctagtacagc
     1141 gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga ttgccgaaat
     1201 aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa atacttcctg
     1261 gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg tttcttctac
     1321 ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt gtgatttcgg
     1381 cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga ctaacgtcaa
     1441 ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg cactagttac
     1501 aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat tgattgctcg
     1561 agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac gattcatttt
     1621 ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg tcataataaa
     1681 ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg tcgcaatgat
     1741 ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt gttgcatctg
     1801 ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca agacctttgg
     1861 tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga tgatcgcaac
     1921 catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt
     1981 ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc ctgctggtcc
     2041 atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag ttgtgaatga
     2101 acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc tttgccaacc
     2161 agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact tactcgttga
     2221 gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg aagattggca
     2281 tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc tgctgttttg
     2341 actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt tcctcctcct
     2401 tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt agcttgtgcg
     2461 atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata ctgcatattg
     2521 tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat taattctcgc
     2581 aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga accagattcg
     2641 atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc agtcccccct
     2701 atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa tgcaagtttt
     2761 tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt ttttccagca
     2821 aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc caacacttca
     2881 ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc
     2941 ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt accgacttcc
     3001 aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc
     3061 atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt gattgccgta
     3121 tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt cttatcgaat
     3181 gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt
     3241 ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc gatcaaaata
     3301 atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt ttgataagca
     3361 agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa aaacatttgt
     3421 gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga ggccgttcca
     3481 tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc cgtacgcaat
     3541 tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat agcagctaag
     3601 atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata atcgacatct
     3661 ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata taaatctgcc
     3721 agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga gatccgtttt
     3781 acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt cagccaagaa
     3841 agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga tgttgaaaat
     3901 aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg gatcagtaat
     3961 tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc aagcaactct
     4021 tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg actcgtatga
     4081 ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg ataaaaagaa
     4141 gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc gaatcctttg
     4201 aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct gtcacgtctc
     4261 attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg atagaaactg
     4321 cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt taagggtact
     4381 ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt aggcaaatgg
     4441 aaaaatgtct gtgtcggcat atggatcctc ctttctttca agatgacaca ttgtcatatg
     4501 acgctgtgtc attataacac ctcctacctg ttttgcaaga catttgcata gtttttttca
     4561 ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa ttaatgagag
     4621 ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc aatcaattag
     4681 tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag atacaagaaa
     4741 taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg ctgctatctt
     4801 gccaagacaa ttaaaaaatc tagatccaaa gaaactttaa tatattctaa gctatacagg
     4861 caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg ctgtttatat
     4921 cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg ttagattcaa
     4981 aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt tttttagtta
     5041 tttattcttc aaccttatca atatgacttg atagcctttt ctttttctca tagttataac
     5101 aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca
     5161 tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta
     5221 taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc
     5281 ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac
     5341 ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt
     5401 ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa
     5461 attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa
     5521 cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt
     5581 atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat
     5641 cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga
     5701 gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag
     5761 ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg taccttggat
     5821 attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa
     5881 agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca
     5941 taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga
     6001 actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc
     6061 aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat
     6121 ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa
     6181 gggatcaggt tcaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg
     6241 accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt
     6301 atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg
     6361 cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa
     6421 aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa
     6481 cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc
     6541 atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc
     6601 aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag
     6661 ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca
     6721 acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag
     6781 taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct
     6841 acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag
     6901 acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac
     6961 cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct
     7021 gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat
     7081 agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag
     7141 ctactgtctc taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat
     7201 catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt
     7261 gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg
     7321 gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac
     7381 tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga
     7441 ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc
     7501 atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata
     7561 cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa
     7621 atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg
     7681 atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag
     7741 agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta ctatacgggg
     7801 cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc gaactgaaag
     7861 cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa gatcatgcgc
     7921 atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat cttggtacag
     7981 atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta actaccttca
     8041 ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga caattcgctt
     8101 atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa gaaacaggtg
     8161 cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt gaaaatggag
     8221 gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac cgaggacata
     8281 actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac aagcaaatca
     8341 tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat aattttgtcg
     8401 tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct gaaacaggat
     8461 tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt tattttgatc
     8521 gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga ctacgggaag
     8581 aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc gaagaggaat
     8641 atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag aatgtagaaa
     8701 actaatcagc tttccatact atctgaaaag aacatcagga caagactttt ccgtctatcc
     8761 tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg gaaaaggagt
     8821 atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata
     8881 aaggattttg atcactctat cataaaggta atattacatt tatattagat aactctagtt
     8941 tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg
     9001 gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct
     9061 tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat
     9121 gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa
     9181 gtactgactt tcttaagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg
     9241 atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg
     9301 atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga
     9361 tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca
     9421 ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt
     9481 taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact
     9541 atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg
     9601 ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg
     9661 ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt
     9721 ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg
     9781 agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa
     9841 tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc
     9901 ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag
     9961 tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc
    10021 atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt
    10081 ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac
    10141 ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt
    10201 catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt
    10261 atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc
    10321 tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag
    10381 ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt
    10441 tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata
    10501 ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag gatctgagaa
    10561 actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc
    10621 atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc
    10681 aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt
    10741 atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg
    10801 caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag
    10861 cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta
    10921 ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt
    10981 gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat
    11041 cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac
    11101 gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat
    11161 aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt
    11221 caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac
    11281 aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt
    11341 cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa
    11401 tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta
    11461 atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata agcggacctt
    11521 tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt
    11581 actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc
    11641 gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt
    11701 ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt
    11761 gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg
    11821 tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca taagtatacg
    11881 gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca gatgttgttt
    11941 ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta cattgataag
    12001 tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt ttgtatcttc
    12061 tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt ctaaatctgc
    12121 ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat ccatccctaa
    12181 gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa gaccgatggt
    12241 tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct gttctaaaat
    12301 attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac gggaagataa
    12361 gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg gtgtattcat
    12421 gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc caacaccagc
    12481 tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg tactacgaat
    12541 aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt gtaacttttc
    12601 tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac cccctcctta
    12661 ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga cttcctagaa
    12721 gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta tgacaagtta
    12781 ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt gataaatggt
    12841 gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa agcaaatttc
    12901 gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt tttatctaat
    12961 aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg ttgttagatt
    13021 tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga caccttcatg
    13081 gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg cttcagtcgt
    13141 tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga aatctgccat
    13201 cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg gatcttctct
    13261 atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa gcgtcggatc
    13321 tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct cgtaagtata
    13381 atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc gatcaatcgc
    13441 tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca aacgttgtac
    13501 agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg taaaagcgtt
    13561 atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt gtacttttac
    13621 atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca attccttgta
    13681 ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt gtacaagatc
    13741 tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca ctttgtctcc
    13801 ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta ttccaaaact
    13861 ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga tctcaggtcg
    13921 tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga tgaacccata
    13981 ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga tcgttttcca
    14041 aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta gaagctttgt
    14101 cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca cacctgctac
    14161 ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag taactgctaa
    14221 ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat cgaccatttc
    14281 ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct gtgatcctac
    14341 aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct tttatttgtt
    14401 ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta aaatccgttg
    14461 aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct tgcatacttc
    14521 atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag taaatggacc
    14581 gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt ctttgaatgt
    14641 ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct gttgtgtcaa
    14701 aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc ccatcgcagg
    14761 aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat ggacgattcc
    14821 tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg aagagggatt
    14881 gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt ctgctgtcat
    14941 ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag gatcaaaagc
    15001 gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa gatttgcttg
    15061 gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct tgcaattgat
    15121 gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat atctgatagc
    15181 ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca aacacgtctt
    15241 ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct gctaataacg
    15301 cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt gcctccatag
    15361 cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca tgccccacta
    15421 aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc attgcaccaa
    15481 aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta cctggtgtac
    15541 gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa aaataaccat
    15601 aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat aataccgtga
    15661 ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca aaaatactga
    15721 cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact agttctcctg
    15781 aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt tctaatacga
    15841 tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccgtttggct acactgttcg
    15901 tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt cctggtctca
    15961 aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca gcttcttctc
    16021 ctgctactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc gctttttcga
    16081 ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa ggatcgatag
    16141 gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc tgccaatgga
    16201 cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct ttattcaaaa
    16261 tagattgata caaatgagcg atactccatc tctgcggaat attgtattga aatttttccg
    16321 ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat ggtactagta
    16381 actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac ccaatcgacc
    16441 cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt tttcggtaac
    16501 tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca ttttctagtt
    16561 tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca atcaatacag
    16621 ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct atgacatcta
    16681 acatactttg cgcatttact tccttcataa tgatttaact ccctccttct cgtcaaacct
    16741 gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg tacgtacatg
    16801 tcaagcggtt atttacaaga atacaataaa aaacacttgt tttgtacgta cataagttat
    16861 aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag aaggagagaa
    16921 tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt ggcgcatttg
    16981 gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct tttttacagc
    17041 atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct tcggtcatgt
    17101 taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt ggacgacgaa
    17161 aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct gcatctgccc
    17221 ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta gctgttggcg
    17281 ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga ctaaggggaa
    17341 gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc tatgttgcag
    17401 attatttgct aaaagtgttg cctgaaacga tggcttggcg cgtgatgtta gggctcgctg
    17461 ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca ccgcgttttc
    17521 ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt cggacaccaa
    17581 aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa aagacaaccg
    17641 gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt gccggtattg
    17701 gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat attccgctga
    17761 tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg atcatccaag
    17821 gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag ttcaatcggc
    17881 gaactttatt gaaaataggc ggtagcgtga tgggactttc attcatccta ccagctgttt
    17941 taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt ttatgtattt
    18001 atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcata ggcgaaatat
    18061 tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat tggattggtt
    18121 ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa gaaatcgttt
    18181 tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag atcgtaccag
    18241 aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa acttatccta
    18301 aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag cttcaagaaa
    18361 taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca gcttctcatg
    18421 tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag tggagaggtg
    18481 gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt gcgacgagta
    18541 accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt tctttcactg
    18601 tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt cctttttcaa
    18661 tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga tgcgtcaatg
    18721 ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct acgatcgata
    18781 ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa ctagctattt
    18841 gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt tgtactgttt
    18901 tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc gtcaaaaaac
    18961 gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt ccttgaagat
    19021 cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa tattctgctg
    19081 ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac tcttcataag
    19141 cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa agtgctaagt
    19201 ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc ccataagcaa
    19261 tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg gctaaaagca
    19321 atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac ggaaaaatat
    19381 aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat ggtgtttttt
    19441 ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc agatagccct
    19501 catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca tatatttctt
    19561 gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga aggatctttt
    19621 gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct ttttatttgt
    19681 acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa ggtttcttgt
    19741 gctcataagg catacggaac aggttcacca aattctggaa atccttgctc attccacgta
    19801 aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt ttggttcttc
    19861 tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac cgtaaacgag
    19921 ttgtgaccag gaccaaattg cttatttttt tcagaagaaa cgaatactgg ctccgataat
    19981 ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag caggcccatg
    20041 gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc attgcgagta
    20101 aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata ctcaggtata
    20161 gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga tagataaaga
    20221 ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag ctttccttga
    20281 gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat ttgtcccttt
    20341 tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa catgcggtga
    20401 tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat ataccatttt
    20461 ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc ttcttcatga
    20521 gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt tgcccttctt
    20581 aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc atctgtatgt
    20641 ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag tgtcttcatc
    20701 ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat ttttctatcc
    20761 ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt tttatttttt
    20821 ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg tagtgctctc
    20881 cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca tttgtgcgat
    20941 tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc acataaggaa
    21001 cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc aatacttgac
    21061 ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc catgcttgtc
    21121 ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga caactaatct
    21181 ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat aatgtatctt
    21241 ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta tgtctttttt
    21301 ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc tcacttctca
    21361 aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga tcgaatcgat
    21421 aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt ttagtcaagt
    21481 cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca ttctcataag
    21541 cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc gtagagcttc
    21601 caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag gcttcatttg
    21661 ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg tctagctcat
    21721 tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt attcttaaat
    21781 cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt gcttcatcaa
    21841 ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga ctgatccatt
    21901 taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct aaatcaattt
    21961 tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca gatgtaaagt
    22021 ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag aggacatctt
    22081 gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct gtatagacga
    22141 cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct cctaccgtaa
    22201 aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac ctttcatttc
    22261 ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata aaaataaaag
    22321 gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa agaatatttc
    22381 tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa aatgtgatat
    22441 ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa ataagtaaaa
    22501 ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga ttaacaagaa
    22561 aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt tgctttattc
    22621 cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc gccattacat
    22681 aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg attacccaga
    22741 gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaaat ttagtctcaa
    22801 ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa caaaatattt
    22861 ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc cagccattac
    22921 aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg gtaaagttct
    22981 catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac tattcatcag
    23041 cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga ttttcaaaaa
    23101 aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat ctggtaaagt
    23161 aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag gaatgatcaa
    23221 acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag gaatcatgaa
    23281 gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg attttccttt
    23341 gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag aagtgatcac
    23401 tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga tcgttgtcca
    23461 aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg gaaatgagat
    23521 aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa acgcaataac
    23581 caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa ctgcttttcg
    23641 actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc tcctatttca
    23701 tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt gcaacaatga
    23761 taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct gtaaatcctc
    23821 gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca gcggttgcaa
    23881 catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata caaaaaccta
    23941 aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt gttgcaccat
    24001 ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc aagagaatcg
    24061 tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc atagcaaggg
    24121 ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag taaggaatcg
    24181 cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc aatgaacaaa
    24241 taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt ttagataatg
    24301 ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg gtgaaatata
    24361 aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg aaattttcaa
    24421 aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta atagcaaatg
    24481 ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga gttaaaaaca
    24541 aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata tagtccacct
    24601 catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat aagatatccg
    24661 aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg atcattcaga
    24721 tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt tgcagtaatt
    24781 gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct gctaataatt
    24841 tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta aaacctggta
    24901 aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga tcatttttta
    24961 cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg atactccatt
    25021 ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct tcttttttat
    25081 tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct gtttttttgt
    25141 ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt gctttcaatc
    25201 cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc attaaattgt
    25261 ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc attttttgga
    25321 tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta taatcatcgt
    25381 tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt ccatccttat
    25441 tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc atttctgtca
    25501 attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta aacaaatcat
    25561 tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt ttaccttcta
    25621 cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca tcatattccc
    25681 agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa tctggataat
    25741 ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt ttttcattca
    25801 aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt gttttattaa
    25861 attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca ttccaaaaag
    25921 tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg caagctccta
    25981 aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct ttcatttttt
    26041 gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg tttcaataaa
    26101 caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt tacttgttca
    26161 cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa atcaatcact
    26221 ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc tccacggtta
    26281 actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa atacggcact
    26341 tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa cacctctcct
    26401 tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca agcttctccg
    26461 tttgtttctg tcctgattgg tgcaataacg tttaccaatt gagcaagaca agcaatcttt
    26521 actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa tgcatcttca
    26581 aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc ttgctcatct
    26641 tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat ctgcttctta
    26701 ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat gaaccgatcc
    26761 atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc ataatattga
    26821 tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa ctcccaatca
    26881 ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat tgaatcatct
    26941 acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc cgctgtttta
    27001 tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt aattccaaaa
    27061 gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt aggaaaatta
    27121 caatattcga ctaaattacg agctgcatct actccacgag tacccagatt gacagccata
    27181 ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc aacttcattc
    27241 gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat gggcccaata
    27301 ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat catgggaatg
    27361 ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt agctgatgga
    27421 tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat aaatgaactg
    27481 tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat tgtggcttcc
    27541 ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc gttgtcaata
    27601 atataaatgt taaacttact tattttttat cgattttaat taaccaaaat attaataatg
    27661 attaaaacaa ataatttaat ataactttta ttaacaaata tgttaatata taagaaaaac
    27721 cttttaagag gtgatatata tgcaactaca aataaacgaa gaatccttac cggtatatga
    27781 agcattagct agtaaaacaa ggatcaagat catccagcta ctttccaaaa aaaagatgaa
    27841 tgtcaaagat ttggcaaaag aacttggagt gagcagtgct atcacgacga tgcacgtgaa
    27901 aaagctagag gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag
    27961 ccttcgtgtc gataaaatcg atattagttt tccagaaaaa atattcaatg cttttgatac
    28021 gaaagaaaca tcgataccta ttggtcacta tacaaattat gcaattgaac caacatgcgg
    28081 tttagctacg atccatgatt ttatcgggaa agtggatgaa ccacgctatt ttatggatcc
    28141 tcgaaggatg gatgcccgca ttctctggtt taccagcgga tttgtcgagt atcaggcacc
    28201 taatttttta aatacagagg atacattgga gatgttggaa gtttctgtgg aaatttcttc
    28261 tgaatttcct ttttcaaatg acaattggcc ttcagatatc actttcagcc taaatggtgt
    28321 ggagctagga acatggacga gccctggaga ttttgctgat atccgcggta aatatacgcc
    28381 tgattggtat ccagataatc ttaatcaata cggcttgctg aagacgatcc gaattaccaa
    28441 acacttgacg aatatgaatg gagaaccctt gtcaaacatc acgatcaacg acatccctaa
    28501 agagcaagac acttggcatt tgcgaatcga agtgaaagac gatgccaaac atgtgggggg
    28561 ctgtacttta tttggtaaag gattcggtaa ttacgatcag gatatcaaat taaaagttta
    28621 ctacagctga tatttttctt ttctatttta taaaacaaaa acagccaatc cctatgaagg
    28681 gacggctgtt tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat
    28741 gtcgtttatg ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa
    28801 cttcttttcc ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat
    28861 ctgcaatcat tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt
    28921 ccggtgcatt caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg
    28981 gcaaaatgtc ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag
    29041 catgatctgt ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca
    29101 tacgttgacg tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc
    29161 ttaatacttg tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg
    29221 gctggataaa gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa
    29281 gacgaacagc gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg
    29341 ttagttgcgc caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa
    29401 aagttttaag aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta
    29461 tttcttcttt tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca
    29521 ggctcattta ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg
    29581 ttccatgata gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg
    29641 cggattcaag tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc
    29701 tttgatttcg tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg
    29761 taaaactggg cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg
    29821 gaacatgaag atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc
    29881 ttcagatttc ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt
    29941 tactttttct gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc
    30001 gatcgatact agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt
    30061 tgtcccaaca ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc
    30121 ttcatccaag tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg
    30181 ttcccgaaca cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa
    30241 gttgattttg tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa
    30301 cgtatcataa gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa
    30361 tgcttcataa tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg
    30421 tatcccaaat atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc
    30481 cgctctagtt ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa
    30541 tagtggatct tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg
    30601 gaaattgacc atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc
    30661 cacttggatc aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt
    30721 tgctttaaat tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc
    30781 acgcaagtaa gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg
    30841 ttccaatcct gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc
    30901 aaaaacagca tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg
    30961 atatagatcc gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta
    31021 ctcctcttgg caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga
    31081 aatcttccat tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac
    31141 tccatctgct aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta
    31201 ttttattcaa tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata
    31261 aaataagtag attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata
    31321 taaccaaatg tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt
    31381 tccttttttg cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt
    31441 tggaaaggcg aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc
    31501 acacgttttt aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat
    31561 ggcgagacat caatcagtct cgccttgttt tttattgtat acattagata tggagcaggt
    31621 taccataaag atgattatta gtcgttatta aatagaatcc ttcgtatttt tttgttctat
    31681 cactacaata tctttatttg gacgtttgaa ttgtattccg gcacgttcta gctcctgttt
    31741 cactcctaca tggactttga catcttctcg tgtcattttt aaaacgtgat gaatcgcata
    31801 gtcgatttct tgccttgtga tttttttctt cgtattgatc atcacgactt gctttttatc
    31861 atatgtgtct accaatagca gttgtgttcg atctaacgaa aataatttgt aataattaat
    31921 agccgtattc ccaaaaaaga attccactac tttaggataa aatgtcttaa tatttaaatt
    31981 actattcaca ggtgtttcga ttagcttcat ctcattcctc ttttctatat agtcgattta
    32041 gatccaatct taagttatgt taccatcttt cctagcatta aggaatcggt tacaattgaa
    32101 cctttaaaga caaaaggatt taccttttat cacttaatag tataacataa tttcatgaga
    32161 aaaaaataca attttttctt gaccatctcg ttacgtaaag gattatgctt acatttgtca
    32221 ggaggaaaaa taatggagta cacaataaag aaaatgtcag agatatcagg agtcagtccg
    32281 agaaccttgc gcttttatga tgagattgga ctgctaaaac cagctaggat caattcttca
    32341 ggttatcgga tttatggcaa aaaagaagtc gatcgtctgc aacacatctt attttatcga
    32401 acaatggcat tcaagttgga tgatatccaa gaggtcttag acaatccatc ttttgaccac
    32461 cagaaagctt taatcaagca tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc
    32521 ctcttgacaa ctgtccaaca gacacttgat atgtacgaag gaggaagaaa aatgagtgac
    32581 aaagaaaaat ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga atcctatgga
    32641 caagagattc gacaaaaata cggaaaaaaa aaggttacag catccaatga aaaatggatg
    32701 aatatggata aagaaacgta tgaagaaatg caaactgttg aaaagcagat gttatttgat
    32761 ctcactcttt acttaaaaga accagaagat gaaagcttag cagaccgtat cttccaagct
    32821 cataagaagt ggttatctta cagctggcct gaatatcagc caaaagcgca taaaggacta
    32881 ggaatgatgt acgaagccga tgaacggttc actgcatatt atgacgagcg aagtggtcat
    32941 ggtgcagcaa aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc
    33001 aacaaaaaag tacacgtaaa atgacctaat aacaaaacag cagacaccca agttagattt
    33061 ttatgctaac ttttggtgtc tgctccattc tttcacaagt tttttcatcc tactttaaat
    33121 atattcatcg ttgaataaac gtataaggta ttttcacttt atcctttttt tctgtaagaa
    33181 acaattgaaa tgccatttct ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa
    33241 gaagctcgct gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta tcttgcagat
    33301 agctttgcaa gatgcctgct gcaaccatat ccgaatttgt aaagacacca tcaactccaa
    33361 gctctttaaa aaagaagcct gcttgttttc ctttctctgc atctatacaa tcccaaaaaa
    33421 caagtgattc atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt
    33481 tataacttag cctgccactt cgccccaacg tgatgcccaa ttgctttata ccttgctgct
    33541 tcaaataagt aagaccttct gtaattgatt gttctcgatc aataaagaca cagcttgcct
    33601 gttttccctt gatttcttca caaaaaacta gaggtccata tttttgatat gccaataaat
    33661 catctagtgt attggcacga gacgtaatga tcaatccatc gaaagcttta gctgcaaatt
    33721 cttcaagata ccttcgctct ttttctttct gatagcccgt cggtaaaagt gtcactttgt
    33781 aatcataatc aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat
    33841 tggtataagg cagaatcact ccaatgcttt tcgtttttcc ataacttaac tgtctggcat
    33901 ttgcattagg gatataatcg acttctttca tgaccgcaag tatttctttc cttttttcct
    33961 catctacata tggatgtcca ttgattacac gtgaaatagt agaaactgaa taacctgtga
    34021 gctttgcgat atctcgaatg tttgccactg ctgcttcgcc acctatacaa aaagattttc
    34081 ttgtaaatag tctaccacag ctggaccgca ttcaaaaaac tgatacattt ttccttcatg
    34141 cgaaaaactg atccgctgcc cgccccaata tatatctttt gtcaaatttt cgatttcatt
    34201 cattggtatt tcttttacta tattcctatt caacccttga aaactgtaca acgttaatac
    34261 ggatcccttg aactcgacaa acgcataaaa ataattgttc gttgtgccat ctgttatctc
    34321 tacataagta ttcattgcaa gccctccttt tgatactttg agtatagtgt atgaaacgcg
    34381 tctcatagca aggagtttgt tttgccaaag aaaagtttta ttcctctttc attattacca
    34441 aagtctctta ttttaaattg aatacattat aatgatactg taaatatatc tttattatta
    34501 gtgaatggat atttactttt aatagataaa atattataac tattaaaaat aactattttc
    34561 attataatta caca
//
LOCUS       DACEKH010000021        33615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEKH010000021 DACEKH010000000
VERSION     DACEKH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.021"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            76..1737
                     /locus_tag="IW662_000937"
     CDS             76..1737
                     /locus_tag="IW662_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0865778.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1807..2154)
                     /locus_tag="IW662_000938"
     CDS             complement(1807..2154)
                     /locus_tag="IW662_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ0865779.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2238..3179
                     /locus_tag="IW662_000939"
     CDS             2238..3179
                     /locus_tag="IW662_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0865780.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3180..4223
                     /locus_tag="IW662_000940"
     CDS             3180..4223
                     /locus_tag="IW662_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0865781.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4229..5299
                     /locus_tag="IW662_000941"
     CDS             4229..5299
                     /locus_tag="IW662_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865782.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5289..6242
                     /locus_tag="IW662_000942"
     CDS             5289..6242
                     /locus_tag="IW662_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0865783.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6254..7264
                     /locus_tag="IW662_000943"
     CDS             6254..7264
                     /locus_tag="IW662_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0865784.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7549..8370)
                     /locus_tag="IW662_000944"
     CDS             complement(7549..8370)
                     /locus_tag="IW662_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0865785.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8467..8745)
                     /locus_tag="IW662_000945"
     CDS             complement(8467..8745)
                     /locus_tag="IW662_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865786.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9013..11022
                     /gene="metG"
                     /locus_tag="IW662_000946"
     CDS             9013..11022
                     /gene="metG"
                     /locus_tag="IW662_000946"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ0865787.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11501..12088)
                     /locus_tag="IW662_000947"
     CDS             complement(11501..12088)
                     /locus_tag="IW662_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865788.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12263..13585
                     /locus_tag="IW662_000948"
     CDS             12263..13585
                     /locus_tag="IW662_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0865789.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13740..14516
                     /locus_tag="IW662_000949"
     CDS             13740..14516
                     /locus_tag="IW662_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0865790.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14509..15078
                     /gene="rnmV"
                     /locus_tag="IW662_000950"
     CDS             14509..15078
                     /gene="rnmV"
                     /locus_tag="IW662_000950"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ0865791.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15071..15955
                     /gene="rsmA"
                     /locus_tag="IW662_000951"
     CDS             15071..15955
                     /gene="rsmA"
                     /locus_tag="IW662_000951"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ0865792.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16406..17035
                     /locus_tag="IW662_000952"
     CDS             16406..17035
                     /locus_tag="IW662_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865793.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17159..18268)
                     /gene="ugpC"
                     /locus_tag="IW662_000953"
     CDS             complement(17159..18268)
                     /gene="ugpC"
                     /locus_tag="IW662_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ0865794.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18477..18899
                     /gene="mgsA"
                     /locus_tag="IW662_000954"
     CDS             18477..18899
                     /gene="mgsA"
                     /locus_tag="IW662_000954"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ0865795.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19222..19758
                     /locus_tag="IW662_000955"
     CDS             19222..19758
                     /locus_tag="IW662_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0865796.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19798..21582)
                     /locus_tag="IW662_000956"
     CDS             complement(19798..21582)
                     /locus_tag="IW662_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865797.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     EEKPDLPDAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGA
                     GKSTLVNLLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLR
                     FGNPEASDAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMIS
                     NPPMLILDEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGA
                     IVEMGTHDELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21585..23300)
                     /locus_tag="IW662_000957"
     CDS             complement(21585..23300)
                     /locus_tag="IW662_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865798.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24079..24303)
                     /locus_tag="IW662_000958"
     CDS             complement(24079..24303)
                     /locus_tag="IW662_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865799.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24503..27271
                     /locus_tag="IW662_000959"
     CDS             24503..27271
                     /locus_tag="IW662_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0865800.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27352..27771
                     /locus_tag="IW662_000960"
     CDS             27352..27771
                     /locus_tag="IW662_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ0865801.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27783..28253
                     /locus_tag="IW662_000961"
     CDS             27783..28253
                     /locus_tag="IW662_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0865802.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28270..29049
                     /locus_tag="IW662_000962"
     CDS             28270..29049
                     /locus_tag="IW662_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0865803.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29036..29857
                     /locus_tag="IW662_000963"
     CDS             29036..29857
                     /locus_tag="IW662_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0865804.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            29874..30923
                     /locus_tag="IW662_000964"
     CDS             29874..30923
                     /locus_tag="IW662_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0865805.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            30936..31940
                     /locus_tag="IW662_000965"
     CDS             30936..31940
                     /locus_tag="IW662_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0865806.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32573..33592
                     /locus_tag="IW662_000966"
     CDS             32573..33592
                     /locus_tag="IW662_000966"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0865807.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        10966 a         5968 c         6880 g         9801 t
ORIGIN      
        1 aattattcat taaactctta aacaacttca gaaaatatta attattctga aaattccaat
       61 aaaggggtac gtaaaatgaa aaagaaattt acatttggtt tggttgcggt atgcggcctt
      121 gtacttgctg gatgttatgg cggcagttca gccacaagca gtagttcagc aaaaagcggt
      181 tcagcagatg gaggaggcgt atttaactta gtcgtgcctc aagaaatgcc tacagctgac
      241 ttatcagttg caacagatac aatcagtttt acagcattga ataacgttta tgaaggaatt
      301 taccgtcttg atgaagacag caaaccacag ccggcaggag ctagcgagtt ggcagaagtc
      361 agtgaagacg gattaactta caaaatcaaa ttacgagaag acgcaaaatg gtcaaatggt
      421 gatcctgtta cagcagcgga ttacgtttat ggctggcaac gaacagtaga cccagctaca
      481 gcttctgaat atgcttacct gtatgcgcca gttgaaaatg cagaagacat tacagctgga
      541 aagaaagata aatcagaact agggatcaaa gcagttggtg actatgaatt agaaatcaaa
      601 ttgacgaaac aaacaccata tttccaatat ctgcttgctt tcccatcatt tttcccacaa
      661 aaacaatccg tagtggaaga aaatggggat gcctatgcat ctgcaagtga taaagctgtt
      721 tataatggac cattcacatt agaagatttt gatggaccag gaacagatac agaatggact
      781 tataagaaaa atgatcatta ctgggacaaa gatacagtaa aactttctga aatcaaagtc
      841 agtgtagtaa aagaatcttc tactgcattg aacttattca aagatggcca agcagatgac
      901 gtgatcttgt ctggtgagtt agcacagcaa aacgcaaacg atccagctta tacttctgtt
      961 aaagaagcgc ggacaagcta tatcgaattc aatcaacgtg aaaagaattc accattcaac
     1021 aatgtgaacc taagaaaagc tatttcttat tctatcgatc gtgaagcttt agtaaaacaa
     1081 gtactaggtg acggatcagt tgtctctaca ggattgatcc catcagatat ggtaaaaagt
     1141 ccaacaacga acgaagactt tgcaaaagaa gctggaaaat tagtttctta tgacaaagat
     1201 aaagcaaaag aatattggga aaaagcgaaa aaagaattgg gcgttgattc tttagaattt
     1261 gatttgatgg cttctgatga tgattcatcg aaaaaagtaa tcgaatatgt tcaaaactca
     1321 atccaagaaa acttggatgg cgtaacagtc aaaccaacac ctgttccatt ctctgtacgt
     1381 cttgaccgct caacttcggg tgattttgac gcagtactag gcggatgggc tgctgactat
     1441 gcagacccaa gcagctttac tgatttgttt gtcacaggta attcatacaa ccgtggtcgc
     1501 tggagcaatg cagaatatga cgaagcagtt aaagcagctt catcaacaga cgctggcgat
     1561 gaacaagccc gttgggatga tttccaaaaa gcaatcaaga tcatctctga tgatatgggt
     1621 gtcgttccgg tgtaccaaaa agcagaaggt cacctagtga atgaaaaaat caaaggtatc
     1681 gttcaccatg cggcaggcgc ttcatgggat tacaaatgga catacgttga agaataatcg
     1741 ttaataccgt gagcatgaga aaaaacgagg ctgcggcaaa tgtcgcaacc tcgttctcat
     1801 attcacttat cctaacaaag aaaaaagcat gaaaatagca gaaagagcaa ttagtattcc
     1861 ggcaatgatc agccaaaagc tgtacatacc gtagctggca gacgagagcg aggaaaattc
     1921 tggttttttc tttcggttcc ttgttcgtgc aagatggacc gatcgctgaa aatgatcgaa
     1981 gaaacccgaa gaaaagaccc ataaaaaacc gccgataatc agaaacggca atgaccataa
     2041 gaatagatca ttagataatt gaatgagtgt aaaggaatgg ttgatgagat tttttattaa
     2101 aataaccaga atcagtagaa aagctactgc gtaggggata gatttttttt tcatttgatt
     2161 gctcctttat ttttggttca ttatctaata atgtagcgaa acatctgtca gaagtcaaaa
     2221 gctaggggga aacattagtg aatagttata tcaaatatgt attaaaacgt gtctttttca
     2281 tgatcatcac gctttggtta attgcaacaa tcaccttttt cttgatgcaa ttgctgccgg
     2341 gcacgccata tacgaatcag gaacgtttga gtcctgaaac aatcgagatg ctaaataaac
     2401 aagtcggttt ggataaacca gttatcgtac aatatgggat ttatctttct aatttgcttc
     2461 aaggagattt tggaatttcc ttccaattta aaaaccagcc agtcgcacat ttgttagctg
     2521 gaagaatcgg accatccttg cagttagggt tacaggcaat tatctttgga acagtcctag
     2581 ggacgatttt aggaacgatc tctgccatga agcaaaatac atgggcagat acatcttcta
     2641 cactcgtagc gatattaggt cgttccattc caaactttgt atttgctgtt ttattacaat
     2701 atatctttgc aatcaaatta caagttttgc caatcgcaaa atgggatggc tttgtgtaca
     2761 caatcttacc aacaattgcg ttggccatgt cgccgcttgc tgactctgct cggtttatcc
     2821 gtacggaaat ggtggaagta ctgcatagtg attatgtgga attagcaaaa gcaaaaggat
     2881 tgagccgttg ggaaatcgcc tttaaacatg gtcttcgaaa tagtttgatt ccattgatga
     2941 ctttattagg accgttagct gtagcattga tgaccggatc actggtggta gaaaatatct
     3001 ttgctattcc tggtatcggg gaacaattcg ttaagtcaat cacaacaaat gattatccta
     3061 cgatcatggc tgtaacgatc ctttactcat ttatgctgat ttttgttatt ttgatcgtgg
     3121 atctattgta cggtctagtt gatccaagaa ttcgtgtttc agaagggagc cgaggataaa
     3181 tggaaatgat accaaagcgt tatgaaacag ttgcagatat ccctgcggat gaatttcttc
     3241 cattacaaaa aaataccgaa gaagaacgtg aacagattac tgcaccttct ttgaatttta
     3301 tccaagattc atggcgccgt ttaaagaaaa acaaagctgc cgttatttca atggttttac
     3361 tattggtcat cattttgatc tctgtgatca caatctttgt ttctcctcat gatccaactg
     3421 cccaaaacgt ggcttatatc aatttgcctc ctcgtattcc gggaatcaat atcgatggat
     3481 tgaatggaaa agcaatggtt ggtggtgaac ttgttgataa atatgctcaa gcaaacgtcc
     3541 cttcaaacgt aaatttcttt ttaggaacag acggtttggg acgtgatgta ttaagccgct
     3601 tatttatggg aatgagaatc tcgttgctga tcgcatttat tgctgcatta ctagatgtaa
     3661 cgattggtgt agcttacggg ttggtttctg gtttgttagg cggacgtgtc gataacgtga
     3721 tgcaacgttt tttggaaatc ttatcaggaa ttccaaacct cgttgtcatg attttgatgt
     3781 tggtcgtttt tgaaccagga attttttcaa tcgtggctgc tatggccatc acaaactgga
     3841 taccaatggc acgtatcgtt cgtgcacaaa caatgaaatt aaaagatcaa gaatatgttt
     3901 tagctggatt gacattaggt gaatcaagaa tgaaaattgc attcaaacat attttgccga
     3961 atatctctag tgtcattatt attcaaatga tgtttagtat tccatctgct attttcttcg
     4021 aagccttttt aagctttatc ggtttaggtc ttacgcctcc gtcagcttca ttaggaacga
     4081 tgttgagcga agggtacaag acattcctgt atctgcctta tctattatgg attccggcag
     4141 taactttatc ggttatcatg attggcttta acttacttgc tgatggattg cgtgatgcct
     4201 ttgatcctaa aatgaaagag tgaagacgat gacgaaaatt ttagaagtga aagacttaga
     4261 aatctctttt gatacgtatg ctggaaaggt acgagcgatt cgcggtgtca attttcattt
     4321 gaataaagga gaaacattgg cgatcgttgg ggaatctgga agtggaaaat cagtgacgac
     4381 cagaagcatc atgcgcttgc tgtcaagcaa cgccaatata gatgctggtc aaatcctctt
     4441 taaaggtaac gatatcgttg gaaaatcaga aagagagatg caaaaaatcc gtggtaaaga
     4501 aatagcgatg attttccaag atccaatgac ctctttagat ccgacgatgc ccattgggaa
     4561 gcaagtcgct gagtccctta gaaagcataa taaagtttca aaaaaagagg gacaaaaagc
     4621 tgcattagaa ttgcttaaat tagttggaat acccgaagct gagaaacgta tcaacagtta
     4681 tccgcatcag ttctcaggag gtcagcgtca acggatcgtg atcgcgattg cactggtatg
     4741 ttatccggaa atcctgattg ctgatgaacc tacaacagcg cttgatgtga ctattcaagc
     4801 acagattttg gagctgctga aagaaattca gacaaaaatc gatacatcaa ttatctttat
     4861 cactcatgac cttggtgtag tagcgaatgt cgctgatcgt gtcgctgtca tgtatggagg
     4921 aagaatcgtt gaagttggaa catcggaaga aattttctac aacccgcaac atccctatac
     4981 ttggggactg cttggttcaa tgccgacatt agatagtgcg aacgatcgat tgtatgctat
     5041 tcctggttct ccaccggatt tattgaaccc gccaaaagga gacgcattct atccaagaaa
     5101 tgaattcgcg atgaaaatcg atgcagaaca agaaccacca ttttttgaac tatcaaaaac
     5161 acacaaggca gcaacttggc tacttgctcc gcaggcaccc aaagtgacac cgccagaaga
     5221 aatccaacgt cgctgggcta tctttaaaga gaaacaaaaa catacttacg gtcaaggagg
     5281 aattgctgat gccaaataaa ctacttgaag ttaaaggatt gaaacagtat ttcaatgtag
     5341 gaaaaaagaa tgaagtacat gctgtaaatg acatcagctt tcacatttat gaaggagaaa
     5401 cattcggttt agttggagaa tctggaagcg gaaaatccac tactggccgt acgatcattc
     5461 gcttgaatga acctactgat ggtgagatat tattcgacgg tcaagatata acgaagatca
     5521 aagataaaaa aggtttgacg aaattccggc atgatgttca gatgatcttt caagatccat
     5581 acgcttcgtt gaatcctcgt atgaaagtca gagatatcat tgccgaagga attgatgtta
     5641 atggactagc taaatcatct gaagagcgtg caaaaaaagt cgatgatcta ttgagaactg
     5701 ttggattgaa tccaagccac ggtactcgtt atcctcatga attttcaggt gggcaacgcc
     5761 agcgtattgg tattgcacga gcattggcag taaaaccgcg ttttatcatt tgtgatgaac
     5821 caatttctgc actagatgtg tcgatccaag cccaagtggt caatttgttg caagatctcc
     5881 aaaaagaaca tcaattgacc tatctattta ttgcacatga tttgtcgatg gttaaacata
     5941 ttagtgaccg aatcggtgta atgcataacg gcttgttatt agagatggga acaagtgatg
     6001 aaatctataa tcacggtgtt catccttata cagaaagttt gttatctgct attcctctac
     6061 cagatccaga ccatgagaga aaacgcagaa gaatcaaata tcaacctgaa ccagatgacg
     6121 gacaagtacg tcaacttcgt gaaatcgcac cagaacattt tgtctatgca actgagcagg
     6181 aagtcccata ttacgcaaaa aaactaaaac gacaaaagga atctcttctg gttgctaact
     6241 aggagggaca tagatgctaa aagcttttaa attccgtatg tatccgacag aagaacaaaa
     6301 acagcaattg attcgtactt ttggatgtgc tcgatttacc tataaccatt tgctgaaaaa
     6361 gcggcagaaa agttggcaac aaacaggagt agccaatttt tcgctcacac ccgctacatt
     6421 aaaaaaagaa tacccattct taaaagaagt agacagtctt gctttagcaa atgcacagct
     6481 taatttggat cgggcttttc gcaattattt caagggacgt gccagcttcc caaagcttaa
     6541 aacgaaaaaa agtatgtggc aatcttatac gaccaacaac caaacccgta cgatctatct
     6601 ggaaaatggc tacttaaagt taccaaaaca aaaagaacta atcaagataa atcagcatcg
     6661 gccagttgaa ggatcgattc gctccgctac gatttctgct cgatataatg aagaatttta
     6721 tgtcgcacta ctttgtgatg tttcgccagt aaaaaaagaa tctttagcta aatggatcgg
     6781 gatcgcctat catccgaaga cattgatcca aacatctcga cctctagaag taactttacc
     6841 aaaatttcac cagacagaag aaaagctgca acatgcacag cggaaattga atgtgaaagt
     6901 ccgctccgct caccatagaa agatcaggtt agatcaggca agcaattatc agaaacaaaa
     6961 gagaaaagta atggatcttt atctgaaaca aaagaaccag cgagaagatt atcttgaaca
     7021 attatctggg aaattggtca aacaatatga ctacttattt gtagaatctt ttccaaaaga
     7081 agaggcacat gctgattttt ctattcacga ctggcataaa ttaataacaa aattgcgtta
     7141 taagtcccaa tggtataata aaaaatttct tttgatcaat accgacggag cagaagaaag
     7201 caattccgta agaaagagcc aagtagtaga aaaaatgggc agacattcgg ttatcaaggg
     7261 ataaaaaatg aatgaaactg ataggagcgt tagggttagc cttgttaata aaaagtctta
     7321 atgacttttg tttctaggaa tttttctttt gaagaaaagc tcactaatta tgacaaacca
     7381 ataaaacaat agcagaaaaa gttcgtacga gctttttctg ctattgttaa ttttttattc
     7441 agaaagttta cgaatacatc aaaattctga aacaaaaaac gtaactcaca aagagttgtt
     7501 tttgcgtttt ttcttgaatg attaggtctg aatcttttgc tcacaacctt attcatggtg
     7561 ccccggcttg agctgataga tttttgaagg acggccgatt cctacaggac gttcaccgat
     7621 ctcatcgaaa tattggagca tagctttctt aaaattggaa tgatcgatcg ttttataatc
     7681 gatccctaag aatttagcaa atacttttcg agcttccgta atcgtgaaat ctttacctaa
     7741 aacttgtaga acttgcggct cgtgttccat tttattgact acacggttga atgctttcaa
     7801 gatgatttga ctatggtcaa aagccaaagt atctttgcca gaagactctc cagtgactaa
     7861 atcaagcgag atagccactt ctccgctaga taaatggatc ttgttactat gacgttctag
     7921 tgtgaaccag cgtacttcct tggcatcatc tccggcgatt agcggttctt ctccaataaa
     7981 ggctaagtag ctgactgtga cgacccagcc tcgcggatct cgattaggcg tactgaatgt
     8041 atgcagttgc tcaatatttt cttttgaaat aaccacacca gtttcttcct ttgtttctct
     8101 taagacgctt tctccagttg actcatcctt ttggacaaag ccaccaggca atgcccatga
     8161 atttcgataa ggatgccctt ttctttggat caacaaaact ttcagctgat cttcttcttt
     8221 gttatagcac atcagtacga tatcgacagt caaagagggc ttgtcatatt ctggacgttc
     8281 ctgtttttga taccagttaa gaaattcttc ttcacttgct ttatgttcat aatataattt
     8341 ttcttcttct ttagaagcaa aacggttcat ctgaccacct ccattgacag taaaagtact
     8401 ttttacctta ttgtagcaga ctctgaaaaa aaaccaagga tttacttgaa aaatctaggt
     8461 tatcatttaa atatgaaaaa taaatggaag tatgagattg aataaaaaga aaagcagata
     8521 aaacggcaat actttatacc gctcttcacg aatggcaaag aagccacaaa taacgatata
     8581 tacgtcaatg ataaaacgca aaatccaagc tgctgtcata aaactgttca aatgcccatg
     8641 gaggttaaaa taaccataca tacgaatata aaaatagctg aatattaaat gtgctaatga
     8701 aagaaaagca atactaagtg taaaaaaaag atgtttcttc tgcatgattg ttccagtccc
     8761 ttactaagat agatagtctt tattatacca cgtgtgaggg gagaaataaa aagccgtttt
     8821 ttagtagata tgaaacaaaa caaaaacccg catttattca acctattcat ccttttaaag
     8881 gatagattga acgtagatag aagtcgttta tccagtcttt ttcattttga aaaaaagcta
     8941 acagcatgct ttttttatgg tatactagca aggaaacgaa ttcgcatcat gcgataaagg
     9001 aggaagagcc ccgtggcaga aaaggaaact ttttatatca ctacgccaat ctattatcca
     9061 agcggcaaac ttcatatcgg caactcgtat actacgattg catgtgacgc aattgctcgt
     9121 tataaacggt tgatgggctt tgatgtgttt tatctcactg gagtggatga acatggtcaa
     9181 aaaatcgaaa caaaagcaga agaactaggt gtaaaaccgc aagaatatgt agataaaatg
     9241 gcggctgatg tcaagaaatt atggaaaacg cttgatatca gttatgataa attcattcgc
     9301 acgacagatg attaccataa agcagctgtt caaaaaatat ttgatcgttt attagaacag
     9361 ggagatattt acttaggtga atatgaaggc tggtactctg tttctgacga agaattcttt
     9421 acagaaacac aacttgcaga agtgtacaaa gatgacgaag gtaacgtgat cggtggtaaa
     9481 gcgccaagtg gacacgaagt agaattggta aaagaagaat catacttctt ccgcatgagc
     9541 aaatacgcag accgtctatt ggaatactat gaagaacatc ccgaattcat ccagccagaa
     9601 tctcgtaaaa acgaaatgat caacaacttt atcaaaccag gattagagga tcttgcagtt
     9661 tcacgaacaa cttttacttg gggagtacca gtcaagagtg atccgaaaca tgtggtgtat
     9721 gtatggatcg atgcattatc caattatatt actgcactag gatatggctc cgaagatgaa
     9781 agtctattcg aaaaatactg gcctgcagat gttcagatgg tcggtaaaga aatcgttcgc
     9841 tttcatacga tctattggcc aatcatgctg atggcccttg atttgcctct acctaaaaaa
     9901 gtatttgggc atggctggct attgatgaaa gacggaaaaa tgtctaaatc aaaaggaaat
     9961 gtcgtttatc ctgaaatgct tgtggaacgt tatggtttag atgcactccg ttattatttg
    10021 cttcgtgctg ttccgtttgg gtcagatggt gtctttacgc cagaagattt tgtttcccgt
    10081 gtcaattatg atctggcaaa tgatctaggg aacttattga atcgaacgat tgctatgatc
    10141 aacaaatatt gtgatggact cgttcctgac tatgcctcac ttgtcactcc atttgatagt
    10201 gaactgtcta cgacggctgc taacgttgtc ggacgttacc atgatgcaat ggaaaaaatg
    10261 gaattcaata cagcattggc tgaaatctgg gtgctgattt ctcgtgcaaa caaatacatt
    10321 gatgaaacag aaccatgggt attagcaaaa gacgaagaaa agaaagctga attgaatagt
    10381 gtcatgatcc atttagctga atctttgcgt atcgcagcga tattacttca gccaatcatg
    10441 acagaaacgc cagagaagat cttcaatcaa ctaggattgg actctgaaac aatgaatctt
    10501 gaaggattac actttggcga atttccttca ggaacaaaag tagttgcaaa aggtacacca
    10561 atcttcccac gtttagatat ggaagaagaa gttgctttta ttcaagaaaa aatgtctgaa
    10621 ggtactcaaa caaacgaaga tacagtcaaa tgggatccag aagaaacaga acttgtttca
    10681 acgaaagaaa aacaaatcaa atttgatgta tttgaaaaag tagaactaaa agtagcagaa
    10741 gtcatcaact gtcaaaaagt agaaggggca gataagttgc tgcaattccg actagatgct
    10801 ggagacagcc aagaccgtca aatcttgtca ggaatcgctg aattttatcc tgatccaagc
    10861 gaattgatcg ggaaaaaagt cgtcattgta gcaaatctga aaccacgaaa gatgcgtgga
    10921 caaatcagtc aaggaatgat cctttctgca gaagcaccag atggctcttt acaagtaatc
    10981 gaagcaccaa aatccatgcc aaatggctca gaaatcgcat agaaattcaa agggggtatg
    11041 acactagttg tgtcataccc cctttttccg aaatttgaga agcaattttc gtaaattcct
    11101 tcttattact tgaagccaat catataatac agaaacagag aactatccaa aataaatcct
    11161 agaaaaagaa gtacgagctt cgctgatttt tgttaattta gaataatact aataggaatc
    11221 gtaaaaacag aaaaaaagca taatgataaa gggtcgggca gattttaatt gattcatctg
    11281 gtactctttt ttattgtcct tacattcata aagtttgaat gcaatgactt tattacatct
    11341 atatttatat ctaacagtag cgttgatgtg ttaattataa gttctatcac tttatgttca
    11401 attagtttgt gctcttatat gtgtgattga aaatatagat aaaaaaacaa tcatcggctt
    11461 tgacttcgat gattgttttc tttattttgt aaaaatccta tcacttgatt ttatggtaac
    11521 cttttatttt tgtcttcaaa cggcttggta agagagcagt aagcagtgca aatgggatcg
    11581 aaaagacata ccagaaaatc gtacgtccga taccataatg ttgatagagc tttgtcaaca
    11641 tcacgtgtaa caagatcaac cctaaaagca aggagccagc tataaaccaa tgattatccc
    11701 aatgcatgac ttcactaagt gccatcactc caaaaggata gaaataaaaa tagatcccga
    11761 ctaaggtcat ccgccagttc tttgtaaatg ttccgatatt caataaaaag aaaatcgctg
    11821 aaagcaaaat accgataggc ggaaaaagcc caaccaaaaa ggaatacaac atcccccaca
    11881 agatcatcac aggacctttg atcaatgcaa cgattgcgat gaacaaagtc agcaatatta
    11941 gtttttgtga taacgttgca acagtcatta atccaaataa aattaatgtg aaccaatgcc
    12001 aatgggactg ctgttgttct ttacgggaaa aatcgacccg gttggataaa taatctgcta
    12061 atttcggata agaacgctta aaatccataa actgttcctc cttcgtggaa aaggtaggtt
    12121 tagtatacga aaaataaaaa agaaatacat ccgtcttaag agggagtttt tcactgaaag
    12181 tggagaaaaa agaatggtaa attaaaatat tctcagttat actgaaaatt ataccaaatt
    12241 tcaaagaaga aagaggatgg gaatgaaagg caaactaaaa cgagaaatca atctgtttgg
    12301 cgcattagct accgttatgg gaaccgtgat cggtgcaggc gtcttcttca aaactgctgc
    12361 tgttactgct agtactcagt ctacaagttt gacattgcta gcttggcttt tgggtggttt
    12421 cttaacgatc tgtgctggat tgacagtggc tgaattagct acagcaatcc cggaaactgg
    12481 tggagctgta aaatatattg aagctgcgta tggaaaattg cccagctttt tattaggatg
    12541 ggcgcaaagc ttgatttact ttcctgctaa tattgctgca ttatcgatta tctttgcgac
    12601 acaattgacg aacttattac aattatctac cgattatctt ttgttgattg cagtgatcac
    12661 cgctgtttct gtgacagggt tgaatttgtt agggacaaaa gtcggtgctt ctgtacaatc
    12721 agtgacgttg attgttaaat taataccaat tgctgtgatt gtcatatggg gattattgac
    12781 cccaggacaa ggaactgtcc agttatttcc aatcgaagct ggaaaagatg ttacttttgt
    12841 cgaaggattg agcagtgcgt tgttagcaac gttgtttgct tatgacggct ggttaggtgt
    12901 cggtgcaatg gcaggggaaa tgaaacggcc ggaaaaagat ttgcctaaag caattattct
    12961 tggtttgagt tttgtaacag tcgtttatct attgatcaat tttgtttttt tgaaaacttt
    13021 gccgattgat catcttgccg gtaatttgaa tgcagcttct gaagcatcag acgtcatttt
    13081 tggaggaatc ggtggaaagc tggtcactat tgggatcttg atctcggtat acggtgcatt
    13141 gaacggttat acactaactg ggatccgcgt gccttatgcg atggctttag aagacgagct
    13201 acctttcagt aaacagctga caaagctttc aaagaagttt acggttcctt atgttcctgc
    13261 ggttttccag ttagcagtag cttgtattat gatgagcctt ggttctttcg actttttaac
    13321 agacatgttg atttttgtga tgtggctatt cagtttactg atttttatcg gtgtctttat
    13381 tttaagaaaa aaagcgccgg aattgccacg tccatataaa gtaccacttt atccaatcgt
    13441 tccgattatt gcgattctcg gtgctatttt cattttaggt atgacgatga tgacgcaaac
    13501 gaaattagca ttgatcggta taggtgttac cttgataggt atcccagtct attatcaaaa
    13561 aaagaaaaaa cgctcagaag agtaaaatat ttcaacaaca gacagaaaaa gtacgaacta
    13621 ggtcctctag atcttcgtgc tttttctatt tcaataaaac aagttcttct attttgattt
    13681 ttgctacttc tgcaagtagt gcttatgtgc taaagtaaga atgaaaagga gagataaaca
    13741 tgatatttga ttctcatacc catttaaatg ctgaacagtt caatgaagat attccagaaa
    13801 cgattcagcg agcacaagaa atgggggtaa cgaaaatggc ggtcgtgggc ttcgatacgc
    13861 caacgattga aaagtctttg caattaagcc atgactatcc aaatatatac agtattatcg
    13921 gctggcatcc gacagaggct ggcagttata cgaaagaaat cgaaaataaa ttacaagaac
    13981 aattgacttt accaaaggtt gtagcattag gggaaattgg cttggattat cattggatgg
    14041 aagacccgaa agaagtccaa gataaagtat ttcgcagaca gattgctatc gcaaaagaaa
    14101 tgaacctacc aatcagtatt catactagag aagcacttga agacacgtat caaatcctaa
    14161 aagaagaaga tgttcgagat atcggtggaa tcatgcatag tttcagtggt gattatgaat
    14221 gggcaaaacg atttcttgac ttagggatgc atatttcatt tagcggtgtc gtcactttca
    14281 aaaaagcatt agacgtccaa gaggctgcag caaatgtacc aatcgaacga ttacttgtgg
    14341 aaacagatgc cccttatctg gcaccagttc cttttagagg aaagcgaaat gaaccaggct
    14401 atactcgtta tacagtagaa aagattgctg aacttcggaa tcttccgaca gaagaagtgg
    14461 ctgcccaaac atggacaaat gcgcaccgat tatttggtct aagcgaacat ggataagatg
    14521 aagatccaag agattatagt cgttgaagga aaagacgata caagaaggct gcgggaagta
    14581 gttgatgcag atacgataga aacaataggg tctgcaatca atgaagagat cctgatgcaa
    14641 attgaacatg cccaagagac tcgtggagtg atcgttttta ccgatcctga tttttcagga
    14701 gaaaaaataa gaaaaatcat tatggaagct gttcctcagg caaaacatgc ttttttgccg
    14761 cgcacacaag ctaaaccaaa gaaaaaggga agccttggtg tcgagcatgc aagtgatgct
    14821 gctattttag aagcattgaa aaaggtcgtg acgccagaca tttcccaaac ggctgtttct
    14881 gaaatcacta gacaagacct tttagactat ggtttaatag ctggggcaca agcaaaagaa
    14941 aaacgagaaa aactaggtga cgagttgcgc atcggttaca cgaacggcaa acaattggaa
    15001 aaacgtttga agatgtttcg tattacgccg aaagaactcg cccaagcaat gaaaatagtg
    15061 gaggaacaac atgagtgaat atcgtgaaat tgcgacaccc tctagaacaa aagagatttt
    15121 attgaaacat ggtttttcat ttaaaaaaag tttaggacaa aattttctga ctgaaccaaa
    15181 tattttaagg aaaatcgtag aaactgcgca aatagatgaa catacgaacg tgatcgaagt
    15241 tggaccagga atcggtgcat tgacggaaca attagccaag catgcaaaac aagtcgtagc
    15301 atttgagatc gatgatcgtt tgatccctgt attggctgat acgatgcaac cctatgacaa
    15361 tgtgactatt gtacatcaag atattttaaa gaccgatttg tcaacagctg ttagagaaac
    15421 atttcatgag gagcttccgt taaaagtcgt agcgaattta ccttactata ttacgacacc
    15481 gatcatgatg cattttcttg aatcagattt ggttgtggat gaactagtcg tcatgatgca
    15541 aaaagaagta gctgatcgta tctctgctga accaggaacg aaagcttatg gttcattatc
    15601 gattgctgtt caatattata tggaagccag tttagcattc atcgtaccaa aaacagtatt
    15661 tgtccctcag ccaaatgtag attcagcgat tttgaaactg acaagaagag atataccggc
    15721 agtcgaagtg acagacgaaa aagcattttt ccgattgaca aaagcggcat ttcagcaaag
    15781 aagaaaaacg ctttggaata acttgcagca ttcgtatggc aaagatgacc agacaaaagc
    15841 atggttagca aaaagcttgg aaacagctgg aatcgatcca aaacgtcgtg gggagacttt
    15901 gtcattacaa gaattcgccg cattaagcaa tgcgatgtca gaaaatatgc agtaaaaaaa
    15961 gaggttgtga cagaagttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
    16021 aaaaatagtt cctcatgttt ttcgtttgtt tgggcttatt accgcagatg caagcttttg
    16081 aacaccgttt aagaaagagg ttgtgggcct gccgtttaac tctaagtcac aaggaacaat
    16141 ttcaaaaaac agcttctcat gtttttgagt tagttggtct tattacttga tgcaagcttt
    16201 tgaccactat tatgccgaac aaggagatgt gacacaaatt ttgtcacatc tcctattttt
    16261 gtttcttgat caaaaaattc ttatacttga tactcacact cattttgtat ggatttagaa
    16321 aagaatatgt gtcttgacag ttattagtaa gtgagttata ctaagtcgac atttatgtgt
    16381 caagacatat taggagagag aaaaaatgag aaaaaattca gttcgaaaac taacgatttc
    16441 tgctttattg attgggatgg gggtcattat cccaatggtc atgcctaaaa tcatgattcc
    16501 accagcttcc tttactttgg cgtctcatgt gccattattt attgcgatgt tttttacacc
    16561 aggagttgca gtggctgttg cgctaggtac gacatttggt ttctttttaa caacgccggt
    16621 tatcatcgca ttgcgagcat tgtcacatct tgtgtttgcg ttgattgggg cttggtactt
    16681 gcaaaagcat ccgagtatcg ttttaaaaaa tggacagttt acttttgcta attggcgttt
    16741 ccaagggttc aattttgtga tcggtgtgat tcattcttta gcagaaatgc tagtagtcag
    16801 catcttttac tttattggta atatgcctga aacatattat tctcaaggat atttttatac
    16861 agtattcatc ttgatgggga ttggtgggct tatccacagt ttagttgatt ataatattgc
    16921 ttattttata gcaggaacac tcagcaaata ttttgatatc cccgtattta cggcagcaaa
    16981 aaaagaaaat cggaaagagt cgattcaagg gtacgcgaag aaaacgacat attagacatg
    17041 atctctcaaa aaaaaaactc tcatctagta atggataata aatcctctaa gatgagagtt
    17101 ttttgtccta aaaagagctg caccaacatc ttctggcaca gctctaacag ggggcaattt
    17161 atggaagttt gatgacttgt tctgtttctt tatcaaagaa gtggcctttg ctgatgttga
    17221 aggcaaggtc gactatttcg cctggtttat ggaaatctct tgcatctact tttgagatga
    17281 actcagtaga accagttttt gtgtaaagca tcgtttcagc tccaagtaat tcggaaacga
    17341 caacttctga tttcactacg gcttcaggtg aagcttcaag ggctacttgt tcactgtgga
    17401 tgtcttctgg acgaataccg aagataactt cttttccttc ataacctttt tcaactaata
    17461 ctttgttttt tccttcagga atacgaagct taagcccatg tccatctgtg ataacaccat
    17521 ttttcaatgt cacattaaag aagttcatgg caggagatcc aataaagccg gcaacgaaca
    17581 tattgacggg tgtatcataa acttctttcg gtgtaccgat ttgttggata aatccgtctt
    17641 tcatgataac gattcggtca gccatagtca ttgcttctgt ctgatcgtga gtaacataga
    17701 tggtcgttgt ttcaagacgc tggtgaagtt tggcaatctc agcacgcatg gctacacgta
    17761 atttagcatc taaatttgat aatggctcat ccattaagaa gacttttgca tcccgaacga
    17821 ttgcccgtcc caatgccaca cgttggcgtt gtccaccaga taaagcagct ggttttcgtt
    17881 tcaagtattc tgtcagacct aagatctctg ctgcattgtc cacacgtttt ttgatgtctg
    17941 ctttatcata tttacgtaat ttcaagccaa aagccatatt atcaaaaaca gtcatatgag
    18001 gatacaatgc gtagttttgg aaaaccatgg caatatcacg gtctttaggt gccacgtcat
    18061 tcatgacttt tccgccgata tttagttctc cttcagagat atcttccagt cccgcgatca
    18121 ttcgcaaagt agttgacttt ccacaaccgg aaggaccaac aaaaacgata aattcacggt
    18181 ctttgattga tagattaaaa tcagttacag aataattttc tgcattgtca tattttttat
    18241 atacgttttt cagagccatt tctaccataa tacgttccac actttctttt ttagttttat
    18301 taactattta aagtataaat gaaagcgtta actaacttca atgtcatgtt gaacaagaat
    18361 tttttctttt ttttgtcaat gtgctagttt taagctttaa taaatatcca taagaaataa
    18421 aaactgatac actggagtaa ataagtatgt taacaatatc aaaaggagtg tctcttatga
    18481 aaatcgcttt gatcgctcat gaccgaaaaa aacctatgat ggtgaaatta gcaacagcgt
    18541 ataaaacaat attggaaaaa catgaacttt ttgctaccgg gacgactggg cagaggatca
    18601 cggaagcaac cggcttgccg atccatcggt ttaaatccgg accgttaggc ggggatcagc
    18661 aaattggagc catgatttcg gaggatcagt tagatttggt gatttttctt cgagacccgc
    18721 tggctgcaca gccccatgaa ccagatgtaa cagcacttat ccgtttatct gatgtctatg
    18781 agattccgtt agctacgaat atcggaacag ctgaggtgct actaagagga ctggaagccg
    18841 gcttcctaga ttttcgaaga atcgtccatg aaatagacaa gcatccactt tcattttgat
    18901 ggataagcgt atggaaaaat aacgtttaat gttcgcctat atgtagctat aaaagctaaa
    18961 caaactgata atatgcgata taatacgaac gtattggata atataaacaa taatattagt
    19021 ttttcgtatt tgcagtaaat gtgaaaatat gctatagttt ctgtgggaaa agatcccaaa
    19081 aataatacag agtagaaaat aaagcgttaa gtgctaagag gatgggatgt tgcctcttgg
    19141 acgaaggacg taatgttcgc ggttttattt tcgcattcgc tgcatgatac gatgtagcag
    19201 ctcttaaagg agatgctaaa aatgaagaaa caccggttag acgcacgtat gattgctatt
    19261 atgggcttat tgattgcatt gatggtcact ttgtcccgac tggtagcaat cgagacccca
    19321 tttattaaaa tcagtgtcac atttattcca caagtgatca tgggcattct ttttggtcct
    19381 ttttggtcag ggataggcgc agttttagct gatttagtag gaatggccct cttctcgaaa
    19441 tctgcattct ttattggatt tacattgaat gcatttattg aaggagcaat ctacggattc
    19501 tttttctatc gaaaagagat cacttggaaa aatgccattc tggctacgct tagtgtcacg
    19561 ctgatcatca acttattttt gacaccatta tggcttgcgt tgatgtacca tgtgccattg
    19621 ttcagctggg tcgtttgggc gccccgcctg ttaaaaacag taatatggtt accgattcaa
    19681 tcaattgcca tttattacgt aggacgtagt attccttaca aaaaaatttt aagaagttta
    19741 gcgatccacg ctaaataaaa aacaagcagt cttcccatcc cagaagactg cttgttttta
    19801 ttgtgctgtg aattgtgctt gatagagccg tcggtaataa ccatcttttt gaagcagctc
    19861 atcatgggtg cccatttcga caatagctcc ctgatccatc actaatatct ggtccgcatt
    19921 tttgattgtg gacaggcgat gggcaatcac aaaactggtt tttccttcca tcattgctaa
    19981 gaaggcttgt tggatttttt tctctgtcaa tgtatcgact gagctggtag cttcatctaa
    20041 gatcaacata gggggattac tgatcatcgt tcgagcaatc gtcaacagtt ggcgttgtcc
    20101 atctgatatc tttaaccctt gaccgccaat cttagtatct aatttttccg gcaaacgttc
    20161 tacaaattca tacatataag aggcttttag ggcatcattg atttctgcgt cacttgcttc
    20221 cggattccca aaacgaagat tttctcgcaa tgtactgtca aatagccaag tatcttgtaa
    20281 caccattcca aaacttttac gtaatgtatc acgttggata gagcggatgt catggtcatc
    20341 gactgtaatt gttccttttg ttgtgtcata aaaacgcatc agcaaattaa caagtgtgga
    20401 ttttccggcc cctgtttttc ctactatagc aatggtttga cctggataag cagtaaaatt
    20461 gaaatcttcg atcaaacgct gcttggggtc ataagaaaaa gaaacatgat caaaagagac
    20521 ttttcctttg atatttttgg gttccaaggc ataggcatct ggcaaatcag gcttttcttc
    20581 tggcaaatca ggcttttctt ctggctgatc gatcaaatca aatgctcgtt ctagaccaga
    20641 aaaagcggtt tgcagttgtg tcgtgattcc agaaagttcg ataaatggtt tagaaaactg
    20701 acttgagtag atcgtaaagc tggaaatcac accaacagtg attgacgaac cactcttcaa
    20761 tgccaatagg ccgccaacta aaccaatcga taaataagca aggtgatcta caaaacgaga
    20821 agacgggttt gtcaaggaag aagaaaattg tgcccgttgt cctttttcat atagatcagc
    20881 atttagcagt tcaaattgct gttgactagc ttcttcttgc tgaaaagctt tcacgatttt
    20941 ctggttaccg attcgttcgg tcacaaagcc cgagatatcc ccaataatcc tttgctgtct
    21001 atcgaaattt gtttgtgaag cacgagcaac gatccaactc acaaggaaaa taattggtgt
    21061 agaacaaaga acgaccaatg tcagaagtgg acttagacgc atcataaaaa taaacgaaag
    21121 aatgatgatt gccactccag aaaataattg attgaaggcc gcagaaattg caatggaaat
    21181 attgtccata tcatttgtaa atcgactgac gatatttccg tgggagtttt gatcataata
    21241 acgaagtggc aatcgattca gatgagcaaa tgcatctttt cttaattttg caacggataa
    21301 gtaagctaac cgattgctta acacttggat gagccattga ctgactacgt tgattaaaac
    21361 gataccaccg aatagaataa gtaaatggaa cagtcgattg aagcggacct gtccttctcc
    21421 aatcatttga tcgatacttt gaccaatttg taacgtcatc acaactgttg caatcccatt
    21481 gatgattcct agtatcacag ctagccaaat gtcttttgca taggcagtaa ggtaaggaat
    21541 gaatcggcgg agcgtctgca aggataattt ttttactttc ataactattc ttcctcctgt
    21601 gatgcgacga tttcctgata ttctgctgat tgagcaagta attcttcatg ggtaccctga
    21661 gcagcaatct taccttgatc catcactaag atatgatgtg cttcttgaat agagcggaca
    21721 cgttgggaga taataatcac tgtactgcca agataagtct gtagcgcctt acgcaaatcc
    21781 aagtctgttt gataatccaa cgcacttaaa gagtcatcta aaatcagtaa tgctggcttg
    21841 gcaatcaaag ctcttgcaat cgtcagtcgc tgtttttgtc cgccagagaa atttttccca
    21901 ttttccatga cttttgtatc taaaccattt ggaagctgac ggacaaattc ttctgcctga
    21961 gcgattttca gagcttccca acattctttt tctgtggcgt ctttcttacc ccattgaagg
    22021 ttttcacgaa tagtcccggt aaatagtaca gaagtttggg gaacaagagc aatttgctga
    22081 cggagatgat ccagctgcca agaacgaaca tccattccgt ccattataac tgtaccggaa
    22141 ctgacatcat agaaacgcgg aatcaattgt gtcaaagtcg ttttcccgct tccggttgga
    22201 ccaatgattc caaggactgt atttcgcttc agctgaaatg taatattttg gagagaaaga
    22261 ccagaatcag gagtataacg gaagtcaaca tggtcaaaag cgataattgc ttcagaagaa
    22321 aaatcgggag tagtactgac ctcagcttca agaatcgaat tttctgtgtc tagcacctct
    22381 ttcacccgat ttcctgaagc ttcggcacgt gtgaagatca cgacgaggtt tgaaacaacg
    22441 atcagtgcca aaagcatttg attcatataa ttgatcaaag caagaacttg tccttgttgc
    22501 aagctaccaa tatttacttt gatcccgcca actgtcagca aacagatgat cccgacattc
    22561 atgatcaagg ttgtagcagg tgataatagg gcagagatat tggaaacacg taaataattg
    22621 ttcgctagat catttgtgct tttatcgaac ttctctgttt ctttttttgt ctgcgcaaat
    22681 gcgcggatga cacgaacacc gctaagattc tgactaacgt gttcattgag ctgatctagt
    22741 ttttcttgca ctttttggta gagaggaacg gtttttttga tgataaaaaa taaaatgatt
    22801 gagaaaatag gcaataatgc taagaaaaac agacctactt cccagtcgat cacaaaagcc
    22861 atgaccactg acccgatact taaaaacggt gctctgatca ataaacgaat gaccattgct
    22921 aaagctagct gaagctgatt gatgtcgttt gtcattcggg tgatcaaggt atctgttcca
    22981 aaatgattca actcgttgtg agagaaagta ttgatttttt tgattagctg attacgtaac
    23041 tctgtaccaa atccttgtga agctactgaa gcatagtatt ggcaaatgat agcacaaatc
    23101 agaccaatca cagacatagc gaccatccat aagcccattt caatgatttt tcctcggtca
    23161 ttcattttta gtccattatc gatcaaggaa gccatcatta aaggcaggac aagttcgaag
    23221 acagcttcaa gaaatttgaa gaacgggccc aaaatgattt gcttacgata gtcttttgca
    23281 taacgtaata actgaatcat atgattcctc ctattattta tttgtccaca attgaaaaaa
    23341 caagtactac cacattgtag cttaggaaat ttagaatgaa aagagaacaa gccttttcat
    23401 aaatgaaagt tttattatga agaagcttta gaaattgaat agactatttt ttttaaaaaa
    23461 aagaaaatga gaaaacatgc cagtctcttc attcatagga acgtctattt tacttaattt
    23521 ttctataaat gaattctgca taaaaaatat atcaagtaaa tgtttataga tgaaactcta
    23581 tttacttctt ttttaatttg ttcttgcaat cttttttgct acgtaatttt tgtaaaaaag
    23641 tgggttgaga gaaatctaat tgattttcaa agaaggagta tgaaaatgag aaaaatattt
    23701 attcgtttat tgtgggtact atcttagagg aataaataat agtggagaaa actatggttt
    23761 ggcgcagatt ctatcaggtt tgatggaacg aagacaacct cccgacatca agtcaaaagc
    23821 tacttgagat tttgatctga aaataaaaaa agaaaacaag gtaggaacat caatgtttct
    23881 accttgtttt cgtaatcaat gggaaaaaac taagagtggt gtacgtaatc aataaaaagg
    23941 atggtgacaa cagtacctct ttactctctg aaagcatcat aactcgtttt tcctcattat
    24001 gccaataaat tgttgttata ccacatataa ggatttgtaa tagctaaaaa cagacaggaa
    24061 agttgaaatc tgtgaatctt attttgtatg gaaggattta tagaacgcaa aaatcaagat
    24121 aaagatgaat aaaaatcctg tttcggctat atattgcgta tctacgccaa taaaacgagt
    24181 ttgttctgtc atagccatat aaagcaaagg caaaaataca actacagcga gataagatcc
    24241 gatgatttct cgaaaagttt tccaggaagt atgtttcctg tttttatatt tatccgattt
    24301 cattgaatag tcatagcctc cttattcttt catatgttga aattccagta taaaaactgg
    24361 aaggaactgt acgtttttta gaataaaaat tataagggat aagtaggctg ctgtctataa
    24421 taagatatat ttttcaattc tttattaaag attttattga caaaatgaat ccgttttcta
    24481 tgatggagga ggggtgaata agttgttgaa gaaggaaatt ttatgttact tagagaacca
    24541 gacagccttt ttcgatttag aacatatgaa cgaggtcttt acagcacaaa ggttagctga
    24601 tcattttggg gtaaaaagaa acacagtcag tcattatttg aaccagttaa atgatgagca
    24661 actactagtc aaaatcaatt ctcgaccagt gatttatttc cacaaagaag cttttgaaaa
    24721 acaatttttt aagttacgca cgaattttta tttttctatc gatgatctca aagaggaaca
    24781 gccttttttc gcgcaaccaa aagacttatt ctcttttatg atcggatacg acgccagttt
    24841 gaaagaaagt atcgaacaaa tcaaaacagc tctttactat ccagatggcg gattaccact
    24901 tttgattaca ggcgaaagtg gcacaggtaa aagttattta gttaatttgg tctatcagta
    24961 ttgtctgctt catgatctgc tagaagattc tgcaccattc atcactttta attgtgcaca
    25021 atatgcagat aatcctgaac ttttgactag caatttgttt ggacatgtta agggagcgta
    25081 cactggtgca gaagaaaata aaaaaggggc ttttgaagca gcagatggtg ggatcttgtt
    25141 tttagacgag gtacatcgct taacaccaga aggtcaggaa aaactgttca cctatcttga
    25201 tcaaggtgtg atttatcgta tgggagaacc taatttgtca cgccggatca aaacacgtct
    25261 cttttttgct acgactgaag agattactag tcatttctta acaactttca ttcgaagaat
    25321 acctatccga atcgaactac ctgcacttaa tcagcgtagt cgtaatgaac ggctagaact
    25381 gatttactct ttttttctcg aagaacaaag aaaaatgggg agccctttgt cagtcagtgg
    25441 accagctttg tcacttttaa caagtcagaa attatccgga aacataggag agctgaaaaa
    25501 tatcgtcaaa gtttcagcag caaaagcata tgctgaacag cgtgaacagc ctgaaatcca
    25561 tgtcacgatc catcatttgc aaaaagaact acttgcgcag cctattacta aaaatacagc
    25621 acagcaagga atctatatta cgcaagacca gaccttagag caattaatgg agaagcaaca
    25681 gcccgaacag cagcgaatcg ttaaaagttt tgagcaaatc ctaattgatt tcaagaaaaa
    25741 taactgtctg ctatattctt gcgaaggaaa attaaagcaa gaagtgattc aattatttga
    25801 ttttttgctg tttgagacca gtagacaaga aaaacacgag ctgctggttt attggacaca
    25861 atctatccga gagacctttc gacaaatgga aactgcctat caaatcaaat tcgatggaaa
    25921 cagtgtctat gccttgagtt actatctcta tcagcgaagc actatcaaat ggttaccgga
    25981 agaacatgat attatccaac taatcaaaga attagaacaa caagtttctc agtcttatcc
    26041 atctagttat cactatgtcc aacgaattct ggaattaagt aagatcaaat tagatattga
    26101 agacacaagt atggatcgga ttttactgac gatctatcta aaaaaagcga aatggtcaaa
    26161 agaaaaacga ctgccaaaag cgatgattgc tgctcacggt tatgcaactg ctagcagtat
    26221 ggcaaatgta gtaaatcgtt tattacaaga ggatctattt gaatcatttg atatgccact
    26281 agatgtcaca ccacagcaga ttgcttcaga aatccttgat tattgcgaaa acagcgatgt
    26341 ttcaaatgga ttgatcatat tagtagacat gggatctctg aaagaaatcc atcagttttt
    26401 taaaaaacag ttatccgtac cgcttttgat tttgaataat gtgacgacac ctttagcaat
    26461 cacagtaggg gaatgcctgc aaaaaaatgt atcattagaa gagatagcag aagagactgt
    26521 tcgacagatc cagccagaat ggcgactgct ctatccagag gaaaacaaac ccaaagcact
    26581 cattactact tgcttcactg gaatcggtac ggcggtccac ttaagtgaac tgctagaaaa
    26641 aagcttacct acggcttgtc agttgaaaat catcccatat gaatatcagc aattaaaaga
    26701 taagaaaaac agtgacccat tattttccat atacgaagtc gtaggaatga ttggcaccac
    26761 agatcctgat atcaccaata tcgactatct ttcattagaa gacctcatct cgggggaaaa
    26821 gatgtctgta ctagctgaat ggttggacac tactatgaat gcttcagaga aggaaatatt
    26881 caatcagcgc atcattcgta atttttcatt ggaaaaagta ctggattccg tgactattct
    26941 agatacagag aaggtgatgg gagaaatcga tcattttatg agagaattag aggtgcagtt
    27001 gaatcgaagc atcagcaatc ccaaaaaatt agcgttgtat gtccatgtga gctgtctcat
    27061 tgaacgattg atccgacaaa tgccaatcga aacctatcaa ggattagaga aatggaagca
    27121 gtgtcaaaaa gaagggttat ctgctattaa atctgcgttt agtgtcattg aagagcatta
    27181 tagtgtcatg atccccaatt ctgagattgc ctatatctat gatattttga gtgaagatac
    27241 agagtttctc tcgatagaag aagaattttg aacataagca agttgttgcc actaattttg
    27301 gcatgcaact tgctttttta gtatgtgtaa gtgaaaagag gagatgaaaa agtggataga
    27361 gaaattatct tagcttccca tggaaaattt gcttcaggga tattagattc attagagctg
    27421 atctatggga aaaatcattc tattactgta ttcgattgct atactgatga gaattttgat
    27481 ttagcggaaa cagtaaggca gctttttatg aggtacaaag ataaagaaat cattgttctg
    27541 acggatcttt ttggcggaag tgtcaacaac gaattcttac aatatatcaa tcgggatcat
    27601 gtctatctcg ttgccggatt gaatttgcca ctattgattg agctagtatc tcggttggac
    27661 gctgaagtag agacaactgt tttgatccag caggtgctaa ctgattcaaa aaaaatgatc
    27721 cagttttgta acgagcggct caatcagaaa atagaagaag acgaatttta ggaggaagaa
    27781 aaatgattgt attgacaaga gtcgaccatc ggttattaca cggacaagta gctttttcat
    27841 ggacacaaac aataggcgca gactgtattt tgattgctaa tgatgatgta cctacgaatg
    27901 agatacgaaa gacaacgatc aaattagcca agccacaagg agtcaaacta gtgatcaaat
    27961 caattgatga ctcgattgcc gcgttgaaaa gcggggtgac agataaatat aagttattca
    28021 tcgtagtgga atcgatcgag gatgcatata agctggcaga agcgtatcca gaaatcaaaa
    28081 ccatcaactt aggagggatc aaggcaaaac ctggtactcg cagcatcgga aaagcggtaa
    28141 atattttacc agaagaggag gaacgcttga aacgattaat tgacaaacaa atagaagtag
    28201 aaatccgtca agtaccagca gataaaaaag tggatgtaag agaagttttg taagaaagga
    28261 agaacctcaa tgattgtaca agcagtctta ttaggaattg ttgcatttgt cgcacaatca
    28321 gaatatgcac tagggacatc gttgctttct cgtccgattg tgacggggct gtttacagga
    28381 atcgtgttag gtgatgtaaa aactgggatc attatgggcg caactttaga gttagcattt
    28441 atcggatcgt tctcagtagg tgcttcgatt ccacctgatg ttgtgaccgg tgggatttta
    28501 gggacggctt ttgctattac agctggagcc ggtacagaaa ccgcgctttt actaggtttg
    28561 ccgattgcta cgttgacatt gatattgaaa aatatttact tagggctttt gattccaatt
    28621 atgaaccata aagcagacaa ttatgcagaa gaaggaaatt ataaaggagt cgagcatatg
    28681 cacttgcttg ccggtatcgg tctttccttg atgcttggtg ttgtggtaat ggtttcttat
    28741 atggtgggaa gcaatacgat cggaaatcta ttaaatatga ttcctgactt cgttcaaaag
    28801 gggttgtcag tagctacagg gttgattcca gctcttggtt ttgcgatgct tgctcgtttg
    28861 ctgatcaaca aacaagttgc accatatttt ttcttaggtt ttgctatagc ttcttactta
    28921 gagattcctg tgaccggagt agctattttt ggtgcgattt tagcagttgt ggtagtcaat
    28981 attatgaatg tccgtcaact accacttcaa acaactgcag gggaggtaga agaagatgaa
    29041 gaattctgag atcaaaaaca gcaaaataac gaaaaaagag ctaaatcacg tattttggcg
    29101 ttcttttcaa atggagtttt cttggaatta tgaacgtcag atgaatatgg cttatgttta
    29161 tgcaatgatt ccgattttga aaaagttata tcagtcgaaa gaagaaatgg cagcagcttt
    29221 gaaacggcat ctagagtttt ttaacacgac cccacatatc gttacactga ttttaggaat
    29281 cacggctgca atggaggaag aaaataagga tgatcctgaa tttgatgtga ctgcgatcga
    29341 tagtatcaaa acatctttga tgggacctct tgcagggtta ggagattctt tcttttgggg
    29401 aactttgcga ttgattgcca caggtgtggg tacctcttta gccatgcaag gaaatatttt
    29461 aggccctatc ttgttcattt tgattttcaa tattccgcac attctgtttc gctatctagc
    29521 aacaggttgg ggatatcgct taggaacggg ttttttgaaa aagatccagg caaacggtat
    29581 gatggaaagt ttgacattag gcgcgtctat tattggactg atggtcgttg gagcaatgac
    29641 tgccacgatg atcaccatca atattccttt aaaaatcggt tcaggagaaa atgctgtgac
    29701 tgttcaaagt atttttgatg atatcgttcc aaatatctta tgtcttggag catttggcat
    29761 agtgttttat ctattgaaga aagaagtgaa accattaacg atcttgatcg gactagcttt
    29821 ctttggaatt tttggttcgt ggatagggat tttttaaaaa aggagcagag aaaatgttta
    29881 cgatgcaaga ttatatctat gaagaaaagg aagtactaag tacgatcttg aaaaagaatg
    29941 atttttcgac aagagaacat atgaaaaaaa cagtgaattt actgatctta gcaacgggtt
    30001 catcttataa cgcttgttta gcagcgaagc cagcattgga atcttatggt gatcttactg
    30061 tcgatattca agaacctttt tattttttca attatggaaa actttctcct tcaatcgata
    30121 ctgtgattgc tgtgtcacaa agcgggaaaa gtgcttctac tgttgaagca gtaaaaatga
    30181 tacaaaaaca agggttacct gttgttgcta tcacaaatga cgtgcaaagt ccacttgctt
    30241 tagaagctgc acaaattatt gatttaggtg ccggggtaga atcagtaggc ttcgttacaa
    30301 aaggctattc tgcgactgtt ttacagttac tactcttagg gataggaatt ggaataagta
    30361 aaaataaaat cagcaaaaaa atagaacaag actacatgca acagttgcgc aaaataatca
    30421 accatttacc agcgattatt caaaaaacgg aagaattttt tgatggatat caaagtttat
    30481 ttcgtttagc acagcgtttc gtcaccattg gatatggtcc aaactgggga acagctaaag
    30541 aagcagagac gaaattgact gaaacaatcc gcgttccgtc acaaggattt gagttagaag
    30601 cttatatgca cggcccatac ctggaggcag atgcttcgca tttgctattc tttattgaag
    30661 gggaaagtgt aaataaggaa cgttcgcaaa agttgcaaag atatatgagt cggtacgttg
    30721 gagagactct caccattacc acaaaaaaag caagaaacga gaaaacactt ggattagcaa
    30781 tagaatgtga tgaatatctg tctgtattag cgcttgtcgt tccgtttcaa ctattcgctt
    30841 ataaaaccgc agtagctaaa ggtattgact tgaacaaaaa gatttttgaa gactttgata
    30901 cagtcttgaa aagtaaatta taagaggtgg aaatcatgct gaaattcaat gaagaagaac
    30961 aaatcaaagc aataaaaggc gcactagctt tacgtccgca agtagaagaa atcatcgata
    31021 aactttatgc ggaaaaattt gacgctgtct attatctagg aatcggtgga acgtatgctt
    31081 cctcgatgca agctgtcact tatatgaatg ggaaaagcaa cttgcctgtc tttgtacagc
    31141 atgctgcaga atattacact actggaaaca aaagattaac aaaagattct atcgttattt
    31201 tgtcctctgt taccggtaca actcaagaag tagtgaaagc agtagaacaa atcaagaaag
    31261 taggagcgac actgattgga tttatcgata aagcgaacag taagttgtct cagctatgcg
    31321 attttgtggt cacttatcca gctcctggaa cagaacaaat caaatttttt atggcagcag
    31381 atcgattgat gtatttgaat ggagaattcg aagcttattc agaatactat gaacagctcg
    31441 aacgttattt accaacaggg ttagtggaag cagagaaaaa agcagatgca tttggcttat
    31501 catttgcaga aaaacatcga cgtgatagta tgcattattt tattggagca gggaatcaat
    31561 ggggagctgt ttattcatat gccatgtgtt actgggaaga acaaagctgg cttccatcga
    31621 aatctatcca tgctgccgag tttcttcacg ggacgttaga gatcgtagaa gaaacaaccc
    31681 cagtcacatt atttttaggc gaagatgaac agagagtgtt atctgaacgt gtggctaaac
    31741 ttttgccaaa aatttgcagc aattatacat tgatagacac aaaagactat ccagtagaag
    31801 gtatcagtga aaaatatcgc ggacgtgttt tgtctttctt attgatgcat gtagtgacac
    31861 aacgcatcga tgctcatgtg gagcagttga actgccatcc attagagata cgccgctact
    31921 atcgtcagtt tgattattag gatagactgg ataaaaagaa tataaaaaat aaagagagca
    31981 gcgataactg ctccctttat ttttttaaaa tattggcatc aattacgtta taaaacacat
    32041 tttttgcatt atctacagtt caacttataa tctgtttaat gggacatgtg ggtgccaacc
    32101 attttttgtt atgcaatagt actatttagt tgttgcatgg atccgtagta aaagtccaag
    32161 tacaacgagt tgcgccagtt tacatagcgt tttgtatcca gaatgttgac taaaacaaaa
    32221 gttcctatgt caaccgtcaa cagagacaat ctgtccgttg caggacctga cagatttaga
    32281 aatgttcaat ctgtctctta tgtattttaa atcaatactc actaggagaa gtttcctaac
    32341 ctcctaatag taatgctggt agtaacattg tcaaaactgc ttcctcttag gaacaagcaa
    32401 agcataacga gtgtaaaaat aaaatgtaga ctattgtaac atatcattga ttatttcaag
    32461 taaaaacgat aaaagaagta aacaaacact taaaaacaat ggaaattagt cccaacttta
    32521 agaaatgtat actataatgg aaattatcaa ttatttattt ggaggaatat ccatgaaaca
    32581 aaaaatcgca gttcttggtc ctggatcttg gggcactgct ttagcacaaa cattggcaga
    32641 aaacgggcat gatgtccgta tttggggaaa tgtacctgaa cagattgatg aaatcaataa
    32701 gtatcataca aatcagcatt ttttacctga tttgacgatc ccagaatcga ttatcggata
    32761 taaagaattg gcagaagcag tggataacgc agatgctatt ttatttgttg ttccaacaaa
    32821 agcgattcgt tcagtcgcac aggaacttat tggtaaaatg aatactaagc caatcattat
    32881 acatgcaagt aaaggactag aacaagatac gcataaacga atctcagaag ttttggctga
    32941 agaaattcca gaagaaaaaa gacaggcaat tgttgtatta tctggtccta gccatgctga
    33001 ggaagtggca gtacatgata tcacaacaat tacagctgca agtattgatc aaaaagcagc
    33061 agcctatgta caaaaactgt tcatgaatga ttatttcagg atttatacaa ataatgatgt
    33121 gattggcgta gaaactggtg cagcactgaa aaatatcatt gcaattggtg ccggcgctat
    33181 ccatggatta ggattcggag atgatgcaaa agcagcaatc atgactcgag gattggcaga
    33241 aatcagccgt cttggtgtcg cgatgggtgc caatcctctg acatttatcg gtttgagtgg
    33301 tgtgggggac ttgatcgtta cttgtacaag tgttcattct cgcaattggc gtgccggtaa
    33361 cttgttagga cagggacata agctggaaga agttctagaa aacatgggaa tggtcgttga
    33421 gggtgtggca acgacaaaag cagcagtaga gctagctcaa cagctaggtg ttgagatgcc
    33481 aattactcaa acaatctata atgttttgta taatggagaa gatatcaaaa aagcagcaaa
    33541 agaaatcatg ttacgtgacg gaaaaatgga gaatgaattt tctcttcgtt aaatagatga
    33601 tcaatagtta atcgt
//
LOCUS       DACEKH010000022        31123 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEKH010000022 DACEKH010000000
VERSION     DACEKH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.022"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(324..689)
                     /locus_tag="IW662_000967"
     CDS             complement(324..689)
                     /locus_tag="IW662_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865808.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            897..1379
                     /locus_tag="IW662_000968"
     CDS             897..1379
                     /locus_tag="IW662_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865809.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1576..2466
                     /gene="pstS"
                     /locus_tag="IW662_000969"
     CDS             1576..2466
                     /gene="pstS"
                     /locus_tag="IW662_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ0865810.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2791..5262
                     /locus_tag="IW662_000970"
     CDS             2791..5262
                     /locus_tag="IW662_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0865811.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5365..7101)
                     /locus_tag="IW662_000971"
     CDS             complement(5365..7101)
                     /locus_tag="IW662_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ0865812.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7098..7802)
                     /locus_tag="IW662_000972"
     CDS             complement(7098..7802)
                     /locus_tag="IW662_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0865813.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7933..8436
                     /locus_tag="IW662_000973"
     CDS             7933..8436
                     /locus_tag="IW662_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ0865814.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8396..8734
                     /locus_tag="IW662_000974"
     CDS             8396..8734
                     /locus_tag="IW662_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ0865815.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8755..10173
                     /gene="ffh"
                     /locus_tag="IW662_000975"
     CDS             8755..10173
                     /gene="ffh"
                     /locus_tag="IW662_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ0865816.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10285..10863
                     /locus_tag="IW662_000976"
     CDS             10285..10863
                     /locus_tag="IW662_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ0865817.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10867..11388
                     /locus_tag="IW662_000977"
     CDS             10867..11388
                     /locus_tag="IW662_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0865818.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11467..12021)
                     /locus_tag="IW662_000978"
     CDS             complement(11467..12021)
                     /locus_tag="IW662_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0865819.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12274..12549
                     /gene="rpsP"
                     /locus_tag="IW662_000979"
     CDS             12274..12549
                     /gene="rpsP"
                     /locus_tag="IW662_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ0865820.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12561..12812
                     /locus_tag="IW662_000980"
     CDS             12561..12812
                     /locus_tag="IW662_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0865821.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12937..13728
                     /locus_tag="IW662_000981"
     CDS             12937..13728
                     /locus_tag="IW662_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865822.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13898..14419
                     /gene="rimM"
                     /locus_tag="IW662_000982"
     CDS             13898..14419
                     /gene="rimM"
                     /locus_tag="IW662_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ0865823.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14409..15170
                     /gene="trmD"
                     /locus_tag="IW662_000983"
     CDS             14409..15170
                     /gene="trmD"
                     /locus_tag="IW662_000983"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ0865824.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15306..15653
                     /gene="rplS"
                     /locus_tag="IW662_000984"
     CDS             15306..15653
                     /gene="rplS"
                     /locus_tag="IW662_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ0865825.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16026..16688
                     /locus_tag="IW662_000985"
     CDS             16026..16688
                     /locus_tag="IW662_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865826.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            16767..19994
                     /gene="fss3"
                     /locus_tag="IW662_000986"
     CDS             16767..19994
                     /gene="fss3"
                     /locus_tag="IW662_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0865827.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            20108..20422
                     /locus_tag="IW662_000987"
     CDS             20108..20422
                     /locus_tag="IW662_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0865828.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            20435..20809
                     /locus_tag="IW662_000988"
     CDS             20435..20809
                     /locus_tag="IW662_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0865829.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            20819..21163
                     /locus_tag="IW662_000989"
     CDS             20819..21163
                     /locus_tag="IW662_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0865830.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            21234..22586
                     /locus_tag="IW662_000990"
     CDS             21234..22586
                     /locus_tag="IW662_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0865831.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            22646..23788
                     /locus_tag="IW662_000991"
     CDS             22646..23788
                     /locus_tag="IW662_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAQ0865832.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            23813..24067
                     /locus_tag="IW662_000992"
     CDS             23813..24067
                     /locus_tag="IW662_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865833.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            24323..25507
                     /locus_tag="IW662_000993"
     CDS             24323..25507
                     /locus_tag="IW662_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0865834.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            25522..25641
                     /locus_tag="IW662_000994"
     CDS             25522..25641
                     /locus_tag="IW662_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0865835.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            25646..25867
                     /locus_tag="IW662_000995"
     CDS             25646..25867
                     /locus_tag="IW662_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865836.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            25880..26380
                     /locus_tag="IW662_000996"
     CDS             25880..26380
                     /locus_tag="IW662_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0865837.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            26477..28324
                     /locus_tag="IW662_000997"
     CDS             26477..28324
                     /locus_tag="IW662_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ0865838.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            28327..29223
                     /locus_tag="IW662_000998"
     CDS             28327..29223
                     /locus_tag="IW662_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0865839.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            29273..29662
                     /locus_tag="IW662_000999"
     CDS             29273..29662
                     /locus_tag="IW662_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0865840.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            29649..>31123
                     /locus_tag="IW662_001000"
     CDS             29649..>31123
                     /locus_tag="IW662_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ0865841.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPLSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT        10446 a         5300 c         6631 g         8746 t
ORIGIN      
        1 tctttgaata ttatagatgt ttccacctca aattcaatat ctattattgg aatcattatt
       61 gctgcaatta ttacacaact gtttttagtg tttggagaga agaaaagaag taaaaaatag
      121 aaaaactagt gatagttttg gtgatagtga tattaaaaat cagaaaagat agaaaaagca
      181 ggagatagat aaaatgctga tatttcagca ttttttaaga gtataaaaga gaaaaaaagt
      241 aataaatgta tacatgatat aagaaaaaca tatatgtata aaaagaagaa agtagcttct
      301 ctttgtacat atatggtttg tttttatgga tgccgagctt ttttataaga taaggctacc
      361 gcccataacg acccaaggat ttgtggaact aagtaaatac ctgcaagaag caagatcagc
      421 tgggtcggtt ttaaaggatg atagaagtaa cgccattcag taaaattggc gcaaaataaa
      481 taggaagtga cagtagatat aatattggca atgaataaag atataatacg cctgttcctt
      541 tgaaaataat atccagcaaa aagagataaa aatatcaatg tgatgaaccc gcttacggaa
      601 tgataacgaa aatcaagcag taaggcgaaa tgcacataag gaacaaaaga taaaagcaac
      661 ggaaggtttt tccagcaagt ttttatcatc tttcatcacc acctaacaag tgttattatt
      721 ttgttagttt tattgtaaat tgaatcctgc aataatcaat tactttttca ttatttctag
      781 attaaatgaa taaactctca tgccattatc tctaaaaaaa aagtttttcc ccctaatttt
      841 tttagataga aaattgtata gtatgatgag agaaaaagta gtttggggga acgttaatga
      901 gaagaaaagc agtaatcgat cggctttatt tatttttttg gttttttcct atcgttttgc
      961 taccttttac atgggtaaat gctgttgtac aaggtgcgga gatattaggc ctgatccaag
     1021 gaagcggatt tgtggtttct aggaattttc cagctttgac tgttttctat ttagtgataa
     1081 gtgtaggtct cttattacca aaatctctga tcaatcacta caattggcag ctgctttact
     1141 tttcttgctt attagtattt acatctatgt tttctttgct gcctcgcttg attatcggcc
     1201 ctaatggttt gagcgagagt ggattgaagg attcagctta ttcctccatg tatttcttga
     1261 cgatcaaacc atggttctat ttagtaattg cctcatttat tcttgcaaca tttttttact
     1321 tctggacaga aatcaggata aaaagtgaac gatctgtatc tataaaaaat aactattgat
     1381 ggtggatcat cagtaaaaaa gcatatgagc tgcgtaaaat gcatgtttct catatgcttt
     1441 ttttattatt atgtaaagaa tgcgaaaaac gtttacatga tttttacata acttaactat
     1501 atttttacag caaccatacg agggatttac agtcgactga taaactttag ttgtaaataa
     1561 gaaaggagca ataaaatgaa aaaagtattg tttactctag gtatgatggg aattctttta
     1621 gccggatgcg gctcaggatc aggaaataca gatggctctg ccacacaaaa taattcaagt
     1681 gactcaaatg aacaagtaaa aatcgttgcg gtaggttcga cagcattaca gccgttagtc
     1741 gatgcagcac aagaaagctt tgtacaagaa aatccaaatt atcaaatctc tgtacaagga
     1801 ggaggaagcg gaactggttt gagccaagtc gaagctggag cagttactat cggaaactct
     1861 gatgtatttg cagaagaaag agacggagtt gatgcttcaa aattagtgga tcataaagta
     1921 gcagtggtcg ggatggcgcc aatcgtcaat aaagatacag atgtcaaaga tatcaccaaa
     1981 caggaattga ttgatatctt tacaggaaaa atcactaact ggaaggaagt tggcgggaaa
     2041 gatcaaaaaa tcaacgtcgt gaaccgtgca aacggcagcg gtacccgcgc gacattcgaa
     2101 aaatggggac tggatggtgc tacccctgtg cagtcccaag aacaagattc atcaggaaca
     2161 gttcggcagc tggtaagtca aacaccagga gcaatcagct atttagcttt ctcatatctc
     2221 gatgattcca cacaagcact aagtatcgac ggtgtagaac caaaagaaga gaatgtagca
     2281 gataacagct ggggaatctg gtcatatgaa catatgtaca cgaacggaaa accttcccct
     2341 gaagtccaaa aattcttaga ctacatgatg acagaagaaa tccaagaagg acctgtcaaa
     2401 gaattgggct atttaccaat cacaatgatg gaagtagaac gtgatcacga aggaaatatc
     2461 aaataactag cagataaaca tgccacggta gtcgttacta ttaaggaaaa atcaactatg
     2521 tttcccacat atacattttt cctttaattg tgaaaaatag ttcctcatat tttgccgttt
     2581 tttgagctaa taccgagggt cagctgatta aacaccgttt attcgaaaaa aagaactacg
     2641 acatagaact gtcgtagctc tttttttgct atatgacagg aatgaaaggt gtttgcaagc
     2701 ttaatgtgag gaacactttc catatctgtt ctatactgga taggagaagt ttggaaatat
     2761 ccagaaaacg aaatcaatcg aggtagccac atgaagaata aatggataaa tcgaagtaaa
     2821 aaaaagaaag taacacaaag atcatcagat aaagtcatcc gtctttttcc atcgtataaa
     2881 gaaggattaa cggcagatca agtcaaggaa cgaatagaaa aaggtgcagc aaataattca
     2941 gtcgatccca cattcaaaac aaatcagcaa atcgtattgg aaaacatatt tacgtatttc
     3001 aatttgatct ttcttatctt agccattttg ctttgtttag tagaatccta taagaacttg
     3061 acttttttac cagttatcat agccaataca ggaatcgcca tttttcagga aattcgatcc
     3121 aaaaagatct tggacgaact aaatgtttta aatacagcaa aagtcaatgt catacgggac
     3181 gggatagaac aaacaataga tatagaagac cttgtaatag atgacattgt tattttagag
     3241 actggacatc aaattccagc agatgcagag gtcgtggaag gaaaactgca agttaatgag
     3301 gctttattga caggcgaagc agatgaaata accaaagaaa taggcgatcc attaatgtcc
     3361 ggcagtttca ttgtttccgg aaaagcttac gcccgattag acaaagttgg agcagattcg
     3421 tatatttccc agctgacagt caaagccaag acaatgggag aaggagaaca gtcggagatg
     3481 attgcttctc ttaatcaatt gatcaaatgg attgggatca tcattattcc tatcggtatc
     3541 ctgctttttt ctcaaagtta cttttttaat ggaaacacga tcaaagaaag tgtcgttgca
     3601 atggaagcag cattgatcgg tatgatacct gaaggactgt acttgctgac gacgattgca
     3661 ttagcattaa gtgcgactaa actagcaaag caaagagttc ttttacacaa tatgaaaagc
     3721 attgagacat tggcaagggt aaatgtcctt tgtgtagata aaacaggaac gatcacagaa
     3781 aataaaatgt ctgttcaaaa agtgatcgtc tctaaaaaac aagaaataat cagtcagtca
     3841 gatcaattgg aagcatgcat tgctgactat gcaaaagcga tggcaggaga caatgcaaca
     3901 atggaagctg ttaaagcata ttttacaaaa accactgatc aatcctatca ccatgttatt
     3961 cctttttctt ctgtccaaaa attcagcagt gtagcgttgg cagacaaggt gtatgtttta
     4021 ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat atgcggaaga gtttgtttct
     4081 ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt acgatgggat tcttgaagaa
     4141 ccagtattga cagaagcagt cacaccatta ggctatattc tgattgctaa tcctatcaga
     4201 aaagaagcaa gagccacatt tgattacttc aaaaaacagc aagtagccat caaagtgatt
     4261 tctggagata atccgctcac cgtatccaat gtagctgtcc aagcagggat ttcacatgct
     4321 gatcagtacg ttgatgcatc taaattatca gaagaagaat atgaagcagc tgtcgaaaaa
     4381 tatacagttt ttggacgcgt caagccagaa caaaagaaaa tcttcgttca attgataaag
     4441 cgtaacaata cagtagctat gacaggtgat ggcgtgaatg atattctagc catgaaagaa
     4501 gcagactgca gtatagcgat ggcttccgga aatgaagccg ccatgcaggc ctcacaagtg
     4561 gtcttacttg attctgactt ttcacgcatg ccagaagttg ttgcagaagg acggcgcgtt
     4621 gtgaacaata ttgagcgatc agccagcttg tttttagtga agaatatttt ctcctttttg
     4681 ctctcactgt tttcagtgat ctttgctctg acctatcctt tagaaccatc acaaatcaca
     4741 ttgattagtt tgtttactat cggacttcca tctttcttgc ttgctctaga agaaaacaaa
     4801 aagcgaatca gagggaaatt catcatgaat gtgatggaaa aagctgtgcc aggtggacta
     4861 acagatatga tcgtagtcgg tgcgttggtg atctgcgggg tgactttgaa tctaaataaa
     4921 acagatgttt ctacagcatc taccatgctt ttgattgcag tagggttcct cgttctctat
     4981 aaaatctgtt caccgctgaa taaattcaga agtcagatca ttcttttctg tgctagcggt
     5041 aacttttttt cggtcatctt tttgcataag ctgttttcaa tcaccggtat ctcagctgta
     5101 tcacttttgc tgttgagcat tctatttttc tcggcagatt cgatttttag gcatttgacc
     5161 acattggtag aatatttgtt tgaacgtaaa aatgaaaaag agcaaaagcc gtttagtcgc
     5221 gtagctaaaa aatttctatc gatatttaaa cgagaaaatt gacgaaaagt atctcaaaaa
     5281 agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca ggatgagtca tccaacttta
     5341 aaacgcacga gcagttgctc ttttttacat tttcggaatg gtaacggtga aagtcgaacc
     5401 tgttccaaga tgactgtcta cagtaatcgt accattaagg agttgcacgt agtcttttac
     5461 aatcgctaac cctaatccgg tcccgcctga atgtcggctt cgcgccttgt ctacgcggta
     5521 aaaacgctca aagatccgtt cctgatcctc aatgtcgata cctataccaa agtcttggac
     5581 agaaaacgat agatcatctg ttgcctgata gcggataata atctcgctgt cgctttttga
     5641 atattggaca gcattttcaa tcagattttt gataattgga taaaggatat ctgttttagt
     5701 agtaaatggt tcgttctttc caatcaatcg aatcgtcaac cgtttctttt cgatgattgg
     5761 ctgataattt tgaacgattt gctggaaata aggttctgta taaattgtct ggatctcgta
     5821 agaattctcg ccatcttttg acagctggat gatttcccga atcagtttat ctaaccggat
     5881 cgcatctttt tgcatgatct caagaaaaga agtcagagtt tgcgggtcat cttttgcacc
     5941 atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca ggagttttta attcgtgaga
     6001 gacgttgcca acgaaatcct tttgtagctt ttctaaacgt cgcactttcg ttaagtcata
     6061 cgccacccct agaatctgtc cggtaccttc tgaattagta aagtagcgca aactcaagtc
     6121 taatgtctgc cgaccttctg taagggtgat ctcctgatgg atcagcggat gctcaggagt
     6181 cacttgatga atcagctgaa tgagttttgg ctcttgaatg acttctgtgt accgctgttc
     6241 aggttgatag cttaccacgt ttaaatgtag ctgcatcttt ggattaagca gtagtaaacg
     6301 agaatcagca tcatcaatca ggaatacgcc aatcatcagt tcgttcagta acgtatataa
     6361 ttgctcttct gtggtagtac acgcccgata catcttactc atttgttcgc tcaacgcatt
     6421 aatcgtctga tacaattctt cccattgatc gggtgtttgc atgattactt ccgcctgatt
     6481 aggattttgc accatttttt ttagtacggg cagcacggtt ttcaatggct ggtttttttg
     6541 atagatcaga taatagatca ttcctgcaag aacaaggaag aaagacagaa agaagaaaaa
     6601 gacccaacgg cgaaaactct cagttctcgg taaaaaaccg cttgtcggtt ctgcgatacg
     6661 taaaatggct tctaactggc cacttttttt gactggtaat gcgacatata aaagatcctc
     6721 attcaaagta gtacttttgc gcaaagcgct acctaggttc ccaccattca atacggcttt
     6781 gatttcggga cgggagtttc gctgttcatt caatgtttca ttgtttgtgt caaataagat
     6841 ctttccagta gcatccatta aagtaatccg ttcgttcgac tgatggacga attcttccaa
     6901 agcagttttg ttttgcgctg cttggaggtt ttcgacatct aattgccgga tcagcagttg
     6961 tgcttttttt gtcaggtagc tttcttgctg ttccacgatc tgctgttgaa aataatggga
     7021 aatcaattgc cagccaccaa aaaacaaact aactaaaaga ataagaaaga agagacgttc
     7081 ataaattttt ttccatttca tcgttttggt tcctgaaact tatagccaaa tcctctaata
     7141 gtcaccaagt actttggatg ttttggatct cgctcgattt tttctcgcaa atgactaacg
     7201 tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat tccaaatgcg gtctagcaat
     7261 gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat aaacaagtaa ttcaaattct
     7321 ttcggtgtta gttcaatcag atggtcgtct acggtcactt gataattggt caaatcagca
     7381 gtgatttgtc caatcgatag atattcaggc tcagcttcat cttttgattg cttacgaggt
     7441 tcaatccgtc tgaatattgc tttcatacgg gctaatactt cgcgaggact aaatggtttg
     7501 gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc gatccacttg gtcatcttta
     7561 gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac gcagccgctg agtgatctcc
     7621 atgccatcca tcgttggaag catcacatct aaaatgatga aatcaaatga ttgttctaat
     7681 gctaattcta atccttcgcc tccatctgtg gcactggtta ccttgtagcc ttctttttcc
     7741 aaattgaacg tcaagagcgt tacaatagaa ggttcatcat caacaactag tatttttttc
     7801 attcaaatct cctttaaata ggtttcattc tgtttatatt ttagcataga atcagtacaa
     7861 agggctattt tacaaaagaa ataaatggta taataggaag gcactttgct gaatagaaag
     7921 gaagagcagt caatgattgg aatcagtgca tgtttaggcg gtctggcctg tcgctatgat
     7981 ggacagaaaa aagaagtacc agaattgatg gaactagttg aaactggaga agctgtcatg
     8041 gtctgtccag aagtgatcgg cggattacca attcctagag atcccgctga aatcattggt
     8101 ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat ggacgacaag cggcgaagat
     8161 gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc aaaaattgca ggaaaaacac
     8221 attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg gaacccattc gatttatgac
     8281 ggaagttttt ccggaataaa aaaaaatggt gccggagtgg ctactgctta ttttattcag
     8341 caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa ctagaggtga gcagaatgga
     8401 aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt tattcgacct tattgacaga
     8461 aaaacaaatg aactacatgg aattatatta tgcagatgat ttttcattag gtgagatcgc
     8521 ggaagaatac gaagtaagcc gacaagcagt atatgacaat atcaagcgga caagcaagat
     8581 cttggaggat tatgaaaaaa aactccatct tttttctaat tatattgttc gagaacaggt
     8641 attggaaaat atgactgcat atattacaga taagtacccg gaagataaaa aactacttga
     8701 atatgtacag caaattcaag caatcgaaga ataaataaaa ggaatgaaga aaatatggca
     8761 tttgaaagct taactgaacg tctccagcag gcgatgggaa aaataagaaa aaaaggaaaa
     8821 gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc gccttgcctt actagaagcc
     8881 gatgttaact tgcaagtagt caaagatttt actaagcgcg tacgcgaacg tgcggtaggg
     8941 gcagaagtgc tggaaagttt atcacctgca caacaaatcg taaaaatcgt tgatgaagaa
     9001 ttgacgatca cacttggttc tgaaacagca gagttaaata aatcaccaaa aataccaact
     9061 gttatcatga tggctggtct tcaaggggca ggtaaaacga cctttactgg taaattagcc
     9121 aattatttaa agaaaaatga aaatgctcgt ccgctcctaa tcgcagggga cgtatatcga
     9181 ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc tagacgttcc tgtatttgat
     9241 atgggaactg aagtcagccc ggtagagatc gtccgtcaag ggatggagtt agcaaaagaa
     9301 aagaaaaacg actatgtatt gatcgatacg gcaggacgtc tgcatatcga tgaaacattg
     9361 atggatgagt tgaaacaaat caaagaattg acgcaaccaa atgagatttt gcttgttgtc
     9421 gatgccatga ctggtcaaga tgccgtcaac gtagctgata gcttcaacca gcaattaggt
     9481 attacagggg tcgtcatcac taaacttgat ggggatactc gaggcggggc agcactatcg
     9541 atccgctcag tgacaggcgc accaatcaaa ttcatcggtt ctggtgaaaa attgactgac
     9601 ttagaagtat tccacccaga tcgtatggct agccgtatcc ttggtatggg ggatatgctg
     9661 acattgattg aaaaagcgca gcaagactac gacgagaaaa aagcggaaga acttgcacag
     9721 aaaatgcggg aaaattcatt cgatttcaat gactttattg aacagcttga tcaagtgatg
     9781 ggtatgggcc cactggaaga tctaatcaaa atgatcccag gaatgaatca agtccctgga
     9841 atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca aaaaagcgat ggtttattct
     9901 atgacgccag cagaaagagc aaatcctgat ctgttgaacc caagccgtcg tcgccgaatt
     9961 gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa tgatcaaaca attcaaggaa
    10021 tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga acatcccagg tatggaccaa
    10081 atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg cgatgaatcg aatgatcaag
    10141 aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa aaaacagctt cggtacagac
    10201 tgaaaatgtc tactgccgga gcttttctat cagcaatttt tattctttac taatacaatt
    10261 actctaaacg aagaggtggc acagatgaga tgtccgtgga gtgaacaaac agaaagtatg
    10321 cggcaatatc atgatactgt ctggggtgtt cctgaatatg atgaccaaaa gctgttccga
    10381 aaattgatgc ttgatattaa tcaagccgga ttgagctggc agacgatttt aaataaaagt
    10441 gaagcattcg atgaagcata tgatcgattc gatatcaaaa aagtcgcaaa ttatggagaa
    10501 acaaaaatca gtgaactaat ggaaaacaaa ggaattattc gtaaccgaag aaaaatcgaa
    10561 gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag aagaaatcgg cagtttttca
    10621 gagtatcttt ggtcgttttc taatggaaaa atcgttgatt cttcttatca ttcacaagaa
    10681 gaagtcccaa caacaagtcc tctttcagat gaattagcaa aagatctaaa gaaaagagga
    10741 tttaaattta tcggaagcac aacaatctat gcttttttag aagctgtcgg gatcatcaat
    10801 gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac gtatcggcag aaagggcgaa
    10861 taaagcatgc gaaaaatcat attttatggt gctataagtt tagatggcta tttggcaacg
    10921 aaaacggatg atcttcaatg gctttttgac actccaacag gtaagcaaac tacttatgaa
    10981 gctttttatg aaacaatcga tacgaccatc atgggcagaa aaacttatca ggaagcaaaa
    11041 aaatatttgg agaccgaagc aatttatcca gagaaaaaga atttcgtttt ttccacccac
    11101 gcacaattac aattgccaga tgctgaggtc gtaaacgaag atcctgttgc ttttctaaaa
    11161 aatctacaaa tgacagaagg aaaagatatc tgggttgtgg gtggcggtcg attgatcaaa
    11221 ccgctattgg aacatcaaat gattgatgaa tggtatatcc agattacccc tgtattatta
    11281 ggggacggga ttcctttatt ccaaaaagga acatatgaag cgcgttttga aatgctagac
    11341 acgacacgat tcggtgaatt tatcgaattg cattatgtac gaaagtagaa gtaaataaaa
    11401 taaaaaaggc aactatatgc agaaatgtcc tgtacatagt tgcctttttt attaccgtta
    11461 tgctttttac tctgttaatt tgttagaaaa atctagatac gcatctaaaa tcgtttggaa
    11521 gaaacttaat gttccttcaa cgatattacc attttcatca attagattcg caacgtttcc
    11581 gatatatgct tccggttgct gcaaggttgg cacattcaaa aagacaagtg attggcgtag
    11641 atggtggttt gcgccaaagc cgccgatccc acctggtgat gcagtaacga ctaaacctgg
    11701 tttatgatcc caaacactgt gtccataagg tcttgagcct acatctaaag catttttcaa
    11761 tactgctggc acagaacggt tgtattcagg agtaacgaaa acgacaccgt ctagtcccct
    11821 cacttcctca cgaaaacgag tccattcagc aggagcctgt tctggagttt cgagatcctc
    11881 attgtaaaat ggcagatcat cgatttttac aaatacactt tcgtaccctt caggaagcaa
    11941 tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct tttcttaaac taccaataaa
    12001 aaatccaatt ttttttgtca tacgattttt cctccttcaa actatctata ataatcttac
    12061 aaaagataag taactggagc aaataaaaac acttactgac aaaagctaca tagaagatag
    12121 attacttatt tttaacaaaa aagaagtcta aaatcttcta ttttctaaaa aaaatcgaat
    12181 ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa aaaaactgtt acactacatc
    12241 ttgtgaataa aacaatggag gtggaaataa ataatggcag ttaaaatccg tttaaaacgt
    12301 atgggttcta aaaagagtcc gttttaccgt atcgtcgtag ctgattcacg ttctcctcgt
    12361 gatggacgtt tcatcgaaac tgtaggtact tacaatcctt tgaaagaccc tgcagaagta
    12421 gttttaaaag aagacttagt tcttgactgg ttatcaaaag gtgcacaacc ttctgacact
    12481 gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac accacgaagc taaattctca
    12541 aagaaataag gtgaccggat atgaaagatt taagcgactt agtcttaaca atcgttcgtc
    12601 cgctagtcac ttatcctgat caagtcaagt tagacgtcgt agagtcgaaa gacttttacg
    12661 agtacaatct aacagtggct cctgaagata ttggccgtat cattggaaaa caagggcgtg
    12721 ttgctaaagc aatccgcaca atcgtttacg gtgttcggat caatgcaccg aaaaaagtgc
    12781 gtttgaatat catcgatgat aaggaaaagt aaataaacgt gctgtactcg aattttcgag
    12841 tgcagctttt ttgttttttt tattaattga agaaatgatt atttggcaaa acaagcaaga
    12901 aagcgtatcc ttataggagg agggagtgaa cagccaatga gaaagaaaat gttatttatt
    12961 ccatttgctc tattgttaag cgcttgtagt tcggttactc aagaatcaga caatacacat
    13021 ctatcaagca gtatatctga aactgttacc agtccagaaa aaagtagtac caaaacggct
    13081 tccaccactg aacaaacaat ggctacctca aataatgaga aaaagactgc tttagaccag
    13141 ttgaaagaac aacagccaaa tgttcccatg cctctagatg taccagtctc ttcaggctat
    13201 ctcaatatcg cagcgacaca tacaaagcaa ggttattcta ttttatatta cagaacagat
    13261 cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa cgcctattgc aacctatcta
    13321 tatcaatatg ggtttgcttc tagtcaagaa acaatccaag ttttgcagcc atttgagatc
    13381 gacacaaatg gtcagcaagt ggacttaggt tctcgaatca cagggtatca gcaaggtgca
    13441 gcaggttcaa gttttcttga atggcaagaa ggaaactggc gtattcgaat ccgtgggaat
    13501 aatatagaag gccaagatcc cttactatta gctaaagaaa tcgtagctta tttggaagaa
    13561 aacagtctac cagcacctga acaatttggt aaaattactg ttgatatggg tgatacgacg
    13621 aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg cctatacgat cactcatcaa
    13681 gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga ggttgtgaaa tcagcgcgct
    13741 gatctgagcc catcatttaa ctccaagtca caggaagtaa tcaaaaatag cacgccgcat
    13801 ttttgcataa ttttggctta ataccttaaa agaaaactag aattgcctct tcacacatgc
    13861 taaaataaaa cagacaaaaa agaaatgggg atataagttg actgaatact tgaatgtagg
    13921 aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt gttatctcaa caacggattt
    13981 cccagaagaa cgttacaaaa aaggaacggt gctaacacta ttccaagacg gaaaagcacc
    14041 tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat tttgacttat tgagttttga
    14101 aggacatcct tctatcaatg atgtggaaaa atatcgggat ggcatattaa aagtgtcaaa
    14161 agacaactct gtagatctag ctgaaaatga attctattac catgagatca tcggtctgca
    14221 tgtattagaa gatggaactg agttaggtaa agtaaaagaa atcttgtcac caggtgccaa
    14281 tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata ttgcttccat atatcgcttc
    14341 tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt gtagaaattc cagaaggact
    14401 gattgacgat gaagattgat gtattgactt tatttccaag aatgtttgaa ggtcctatgg
    14461 gggaatccat catcggtaag gctgtagata aaggattatt agatatcaat atctctaact
    14521 tccgcgatta ttcagataac aaacatcaga cagtagatga ttatccatac ggcggtggag
    14581 caggtatgct gctcaaagtc cagcccatct atgataacat ccaagccatt gaaaaagctg
    14641 caccggatgt caaaaaaaga gtaattcttc ttgacccagc tggtaaacga ttcgatcaga
    14701 aaatggcaga agaattttca caagaagaac acttagtatt catttgtggt cattatgagg
    14761 gatatgatga acgtatccgc acattagtga cagatgaagt ttccttagga gattatgtcc
    14821 ttactggggg agaattaggt gcgatggtca tgatcgatgc aacagttcgg cttttgccag
    14881 atgtgttagg gaatcagact tcagctcaaa cggattctta ttcaaccggt ttattggaac
    14941 atccccaata tactcgcccg gcagaatata aagggatgaa agtaccagaa gtattgacaa
    15001 atggaaatca taaattgatt gaagaatggc aattaaaaga atcactaaga aggacctatc
    15061 aacgtcggcc tgatcttttg gaaacacttg aatggacacc tcaaatgacg aaatttttag
    15121 aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga agccaagtga ctttttctaa
    15181 aatcttcttt acagtaggtt gataatatgt tagtatttta tggtgagtgc aaaagcactt
    15241 aactattacg atattccgct gtgagaaaag ctcataagaa tatttggaat aaggagaaga
    15301 aaaccatgaa tccattaatc gaagaattaa caaaagaaca attacgttct gacatcccag
    15361 cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt tgttgaaggt actcgtgaac
    15421 gtatccagtt atttgaaggt gttgttatca aacgccgcgg tgctggaatc agcgaaactt
    15481 atacagtacg taaagtttct aacggagtgg gcgttgaacg tacattccca ttgcatacac
    15541 cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt acgtcgtgca aaattgtact
    15601 acctacgtgc attacacgga aaagctgctc gtatcaaaga aatcagaaga taaacacgga
    15661 aaacttcctt ggaaatctga aatttgtgaa aagtccttgt atatcaaggg ctttttcttt
    15721 tttctctatc acagttttaa gccatttttg gctgttttgg accatttttc aatcatattc
    15781 ccttaaaaat tgtcctagat tgtccgacat tgactatcag tgtcgtcaaa atagggcaaa
    15841 atttagtagt cagcttgacg acaaatataa tacgaaaaat accaaaacat ttaccgaatt
    15901 tagcaacttt tgaacccaag tttctcaaag ggtcagctat tagcaaaaaa gacaaaacat
    15961 gtacttttct actaaaaagt tgatgtgata ggcttctatc taaataacta ttaggagttt
    16021 ttatcgtgaa gcaaaatgat ggacgtatca tttggaaaaa tcaaagtgag ttaaaactga
    16081 ttttaacaat taatgaattc atcgaaaaac acggaattac atctagccgt caataccaga
    16141 aaaagctatc agaaaatcca aattctgcac caagtatgtg gttcattaac aaaaaatatg
    16201 gatcatggga gaatttattg attagtatag gacgagaaaa tactgggtat ggaaaatggg
    16261 ctagaatgtc agaacaagaa ttattggaaa tagtagaagc ctttattaag tgcgagaaga
    16321 taacctctca acgaatgtac gaacaaaaat cagtagggaa gaatattcct tcacttagta
    16381 ccattaaaaa gatgttagga gatatacgtc cactttttaa agaaaaaaat gatggatctc
    16441 gatttactga tttcgaactg ctgttagaac taaaaaatga aataatccga ttaaaactac
    16501 aggatgattt atcaatgaca aagtttcgaa aactagttca atccccaaaa ctaccatcag
    16561 ttgatacaat tatgaaaaga acgaacaaaa attgggagga attgatggca gagattggat
    16621 ttgattatcg aagaatcaaa atttataagc agagaaacaa cctatccaag acaaagaaaa
    16681 ctaaatagta tcactctaaa tcagacgttc aaaattaaaa tttggacgtc ttttttatta
    16741 cccaaaaacg aaaggagatt tattttatga aaaagccaag atttaagaat tggcgtttgc
    16801 tcgcaacact gagtctatta tgccaaacaa ttggtggatc gcttggccca acgattgcct
    16861 ttgctgatga aattactcat ccacaaacgg taactgtgga attggatttg gctcaccagt
    16921 atgctgtcga aggtacattt agcgatggtc gtcccatgtc agaagttacc gttccgcact
    16981 atgcagttta caatggtgtg aagcaagata ttttctgtat tgagccaggg gtgccgattt
    17041 acaatgagtt cactcctggt tatgagaaaa atccattgcc tgatatgtcg gagaaagcga
    17101 aattagtttc cgttttatgg aaggaagctg gtacggatgt ggatacacat attgtggccc
    17161 agaagatgat ttggcaagaa gttaatggct acacgcttca ctcaattaaa cgttctgatg
    17221 gtagtgcagt aaatatcgca gccattgagt caaaaatcaa tcaagccatt gcggattatc
    17281 aaaagaaacc aagcttccat aactccacag tgaaaaccgt gttaggtcaa tcgactactg
    17341 tgacagatac gaacggcttg aatttatcgg agtttgatga agttgtcgag aatatcgcaa
    17401 atattgatta tcgtgtgaac ggcaatcaac tagtgattac tccaaatgcc aattcaaaag
    17461 agagtggtga attaacccta aagaagtcag ctaatacagg aacaccggta gcttataaaa
    17521 tggctggtca acaaactttg atggcggggg caattgataa gcccaatacc tacacaatca
    17581 aaattgatgt ggaaactgag ggttctttga agattaaaaa agtcgataaa gaaacaggaa
    17641 atgttgtacc gggaaccgtt ttccatttag actttggaaa aacattacct gcaaaagacg
    17701 tgaccaccga taaagaaggc attgcgacat tggatgagat tcctcatggc gcgaaagtaa
    17761 ccattactga aaaatcagtg ccagctcctt atacgattga cactacacca atcaccgcaa
    17821 ccattaaatc tggtgagact atttctgtga cttcaaaaaa tactcgcgaa aaaggtcaaa
    17881 tcattttgaa taaaatgggg gtggaaacag gaactgatct ttggaacgac aaatactcat
    17941 tagccggaaa tacgtttgcc attcgcaaag acagtccaac cggtgaaatt gtccaagaaa
    18001 tgaccacgga tgaaaaaggt catgcggaaa caccaaaaga gattgccaat gcgttggaat
    18061 tgggaaccta ttatgtgacc gaaaccaaag ctagtaatgg ctttgtgaat acctttaaac
    18121 cagtaaaagt cgaattgaac tatgtcaatc aaaccgtggc tcttgttacc agtaacgtaa
    18181 aagggcaaaa ccaagaaatc actggtgaaa ccactttgac aaaagaagac aaagataccg
    18241 gtaatgaaac tcaaggtaaa gctgagttta aaggagctga atatactctc tttactgcaa
    18301 aagatggtca agcggtcaaa tggagtgagg cttttaaacc agagttagtg aagggaacga
    18361 aagcttctga tgaaacggtg actttgactt tagatgaaaa gaatcaagtt gctgttaaac
    18421 acttagcgat taatgagtat tactggcaag aaaccaaagc acctgaagga tataccttgg
    18481 atgaaacaaa gtaccctgtt tccatcaaaa aggtggatga taacgaaaaa aatgccgtga
    18541 ttacccgaga tgttacggca aaagaacaag tcattcgctt tggctttgat ttctttaaat
    18601 ttgctggatc agctgctggg actgccgaaa ctggcttcaa tgatctgacc tttaaagtat
    18661 cgccattgga aggaaccagt gagattacag atgccgaaga tgaagcaatt acagcttata
    18721 acgagcaact aggttttgat ggctatggca agtttgaaaa tcttccttat ggggattatt
    18781 tacttgaaga agtagaggct ccagaaggat ttcaaaaaat tacgccatta gaaatccgtt
    18841 ctacttttaa agaaaataaa gcggattaca ctaagagtga atacattttt acgattaccg
    18901 aaaaaggaca aaaacaacca attaaaacgg tgaccgttcc ttacgagaaa ctgacgaata
    18961 aagcattttc tgttagcttg aatcgtttga tgctctatga tttacctgaa gaagaagata
    19021 gcttaacttc tcttgggact tggaaagacg gaggcaaaga attgacagac cttgattcta
    19081 ccgagctagt tgataaattg agctataact tgcatgaaat caaagaagac tggtatgtgg
    19141 tagcgcaagc cattgatgtg gaagcgacaa aagctgccca agaaaaagat gaaaaagcca
    19201 aaccagtggt aattgccgaa acaaccgcaa cgttggcgaa taaagagaaa acaggaactt
    19261 ggaaaatttt gcataaatta accgcagaac aagttttgga taaaaccatc gtcttattca
    19321 actatgtgta tgaaaacaag aaaccctatg aagcaggtga tgatccagta gcgaaagatg
    19381 ctagcttgaa caatcaagcc caaactgtca attgtacggt ggaacgccat gtttccatcc
    19441 aaacaaaagc ccacctagaa gatggttcgc aaacctttac tcatggggat gtggtggata
    19501 tgttcgatga tgtgtcgatt acccatgatg tactggatgg ctcaaaagaa gcttttgaaa
    19561 caattctgta tgcattactt ccggataata cgaccaaaga aatttggaaa tctggcaaaa
    19621 ttgattatga agtgaatgac aaagaattca ctaaaaccgt acttgcggaa aaagtggaca
    19681 caagtaaata tccagaaggt acaagtttta ccttcaaaga aattaactat ggcaaagacg
    19741 gaaacatcaa tgggaaacac aatgaggatc taaaagaaaa aactcaaact ttaacaccta
    19801 aaaaagttcc gacaacacca agtgcaccag aacaaccgga aataccaact gttccaagtg
    19861 actctcaaga atctagtcct acagtaaaga cattcccaca aacaggggag aaaaattcta
    19921 atgttctgct gtttattggg tttaccttga tctttgcgac tgctgggtat tatttctgga
    19981 atcgccgaaa ctaaggtgat gtgatgaaac aactaaatag gaaaaaccaa cgtgcgcgac
    20041 cgcctcctaa aaaaagtggt cgcgctattt ttgtatccaa aactaaaaat gaaaagagga
    20101 aaagaagatg gaattgaaat ttgttgtgcc agatatggcc gaaacctttg gaaagataag
    20161 gtatgccggc gaaggcgaag ttttaactga aggatatggg cggaatacta cagtaattgg
    20221 tcgtagttat catttgtatt ccagtaagca acgagccgat gatattgaag tggtggtagc
    20281 tgcggaagcc ggcgaaaagg attttgatca agatcaacca ctaaaagccg tgaatcccca
    20341 tttggttgcc aaaggctatg agattgagaa tcgtgggttt accgactacg tgttgtatgt
    20401 cgatgattta gtgaaagcat aggaggaaaa gaagatgaga ttaacagaag gtattgttgt
    20461 agattcagga ctaacgtttg ggaaattacg attttccgca ttacgccgag aagtacggaa
    20521 gcaaaatgag gatggaacgg ttagtaacga ggtaaaagaa cggacttata atttgaaatc
    20581 ctctgcgcaa ggtcgaatga tccaagttag tatcccagca aatgtaccct tacgcgagtt
    20641 tgcctatgac gcagaggtag agttggtcaa tccaatcatg gatacggtcg ccaattatgt
    20701 tttccgagaa ggaactactg tcaattggtt cattaaggcc gatgatttag tcttgaaacg
    20761 acaaccagat cagggaaatt ctacaaacca aaatgaagga aagaaatagg tgaagcgtat
    20821 ggaaattaat gttgaacata ttttagattg tctcgatcaa tatggaaaag gtgagctaac
    20881 ggaggaacaa ctcactaaag cattaaccta tgatgaaaaa atgtttctga ttatgcatca
    20941 agggttactg gaagtaggta acgatgccga agaagatttt gatgtactga attggttagg
    21001 ggaagaagag tccttcttta tggttatcga agtaaatgaa agtctatgtc gagaagccga
    21061 aaccgtattg gaagaaattg gtgtggaaat gccggatgcc attgaagttt ttttaaagca
    21121 actagtggaa acgaaacaac tccctatagc agtaaatgac tagcataact gcttgaaatt
    21181 ggatcaatga aatcctatga agcttttttc gattggaggt ggaaaccata aaaatgtaca
    21241 aagggcatcg tatacgggca ggagatcagc acttggttta tcactttgtt cttggctggc
    21301 tactcgctct gttcatcggt tggatgagcg tcttttattt tcaagagctt agacaatttg
    21361 atatttctaa gctatcactt tctactatag aaatcgttcg ttctataaaa gatttaatct
    21421 atttgctagg aagtctcgtt ctttcaggaa gcacgatgtt gctttatata cacttttttc
    21481 aggatcattg gcgcagtttg tggcatcgac aaaagctagc ccggatgatt ctagaaaatc
    21541 attggtacga agtaaaacaa actcagagtg aaggattttt caaagacctc aatagtagtc
    21601 gaaccaaaga gacaatcagt tacttcccca aaatctatta ccgtatgaag gatggtttac
    21661 tttctattcg cgttcaaatt tcactgggaa aatatcaaga tcagctttta aagctggaaa
    21721 agaagttaga aagtggtcta tattgtgaac tagtagaaaa ggaactcaaa gactcctatg
    21781 tggagtacac tttactgtat gatataattg ccaatcgaat tgggatagac gaagtagtgg
    21841 cagaaagtgg ggctttgcga ttgatgaaaa atcaagtatg ggcttatgat tctttacctc
    21901 atatgttaat cgctggcggc acaggtggtg ggaagactta ctttttactc actatcatcg
    21961 aggctttatt aaaatctgat gccgagcttt ttgttcttga tcccaaaaat gcggacttag
    22021 ccgatttagg tacggtaatg cctcatgtct attcccaaaa ggaggaaatt tctgcttgtg
    22081 tggaagattt ctatgagcgc atgatgactc gtagcaaggc aatgaaagaa atgcccaact
    22141 acaaaacagg agagaattat gcgtttcttg gacttccacc aaacttttta atctttgatg
    22201 aatacgtggt ttatatggaa atgttaacga cgaaggaaag tgcggtgatt ttgaataagc
    22261 tcaaacaaat cgtgatgtta ggtcgtcaat ctggtttctt tctgattcta gcttgtcaaa
    22321 gaccggatgc gaaatattta ggagacggga ttcgcgatca atttaatttc tgggtggctt
    22381 tgggtcgtat gagtgaactg ggatattcca tgatgtttgg cgaggttgat aaaaaattct
    22441 ttatgaagcg tatcaaaggt cgaggctatg tagatacggg agggagtgtg attagtgagt
    22501 tttatacgcc acttgtacca aaaggatatg atttcttaag ggaaattagc gacattggtg
    22561 gattaaacgt taatacggaa ggataaaata atagtatgta gcctaggagt gtcaagaatc
    22621 ctgggctttt tttacgaggt gatttatgac atttttagat ttattcgctg ggattggtgg
    22681 cttttgctta ggtatggaac aagcgggtca ccagtgtatc ggcttttgtg aaattgatga
    22741 ttttgctcgc cagagttata aggcaatcca tgatacaagt aaggaggtgg aaatgcatga
    22801 catcacaagt gtatcagacg agtttattca atctctcgga ccagtggata tcctttgcgg
    22861 tggatttccg tgccaagctt tttcaattgc gggaaagcgg caaggatttt ccgatactcg
    22921 aggtacttta ttctttgaaa tcgctcggtt cgccgctatt ctccaaccta agtttttatt
    22981 ccttgagaac gtccggggat tactcaatca cgaaggaggg gctacgttcg agacgatcct
    23041 ccgaacgctg gatggattgg ggtatgatgt ggaatggcaa gtgcttaact caaaggccta
    23101 cgttccccaa aaccgtgagc ggatcttcct tatcggacat tctcgagacg catgtaccga
    23161 acaagtattt cctatcattg gatcgtctcc aacatctgat caaaacatca gaaacttatt
    23221 aaatatcaac ccttccaatc gaggaatggg gggacaagtc tatgggtcag atgacgtagc
    23281 gcctaccttg accggtgatg aaggaatcaa aatcgcattg ccggtagatg atggaatttc
    23341 tgtagcgggt atgttactgg gaaattttga acaaggcaat cgggtttatg agataactgg
    23401 aacagcgcct actttgtcaa cgaaacaagg cggaacgaag atcatgcttc ggaaaaacga
    23461 aacagaatat caagaggtga agccagggaa cagtgtgaac ctggcttttc ctaattccac
    23521 aagtcgtcga ggccgtctgg gaaaacaaag tgtgcatacc ttattaacag gagaccaaca
    23581 agcagtcgtc acggatcagt atcaaattcg aaagttaaca cctagggagt gctggcggct
    23641 tcagggcttt ccggactggg cttttgatcg agcttctcaa gtcaattcag atagtcagtt
    23701 gtataagcag gctggaaatt ctgtaacagt tccggtcatt tttgatattg ctagaagatt
    23761 aaaggaggta aaaacagatg atttatgatg agttgattgg ggaatctact gggtgattgg
    23821 caagattcag tcagatccgg aattggaaga agaactgtac cggctcaatt ttgatattcg
    23881 caaaaacggg gtgaaagtcc ctgatgatcc gtatgtaatt gatgaggaaa cggatgctcg
    23941 aatggagctg aatcaagtca tcagtgaatt ggaacgaatt gcggactttg ctaaagaacc
    24001 tgatatccgc caatacttgt ttgagataaa agcagagttg gaaattgagg gtattaccgc
    24061 ggagtaacgg ccaaataaac cactgatagg cactgaccgc gggggatttg cgaagcagaa
    24121 atcctccgtg gtgctgtgcc tgtttatgga cggcgttagc cggccatgat gtctcacccc
    24181 ccgtatctaa caggggggta caaaaacaca aaaaataagc aagcaacgat aaggaatggc
    24241 tgtcctatca aggataataa gcaatttgtg tagatgttaa ctgatttgaa aaagttaaca
    24301 ccttaaattt ggaggtgaga acttggcaca aaggaattta gattatcggc tattaaaaga
    24361 ccggcgtaat gaatatggcg tttcacaaaa taaattagct acgacttgtg gacttagtcg
    24421 cccgtactta aatcagatag aaaatggtgg cgtaaccgca tcgacaaaaa ccatgaggaa
    24481 aatttttgac caactggaaa gtttcaatcc cgagttacct ttaacgttac tgtttgacta
    24541 tgtaaggatt cgctttccca caacggatgc gaggaaaatc attcaagaga ttctccattt
    24601 gaaatttgat tatatgctcc acgaagatta cgccttttac tcctatcaag aacaatatgt
    24661 tatgggagat attgtggtga tgttgtcaca tgaagaagat aaaggcgtcc ttttagaatt
    24721 aaagggtcgt ggttgtcggc agtttgaaac ttttttactc gcccaaaagc gtagctggta
    24781 tgactttttc gaagattgtc taaaagccgg tggcgtgatg aaacgcttag acctggcaat
    24841 caatgatcga gttggcctgt tagatattcc tgatttaacg aaaaaatgtc agaaggaaga
    24901 atgtatctcc ttgtttcgta ccttcaaaag ctatcgttcc ggggaactgt tgaaacctga
    24961 tgataaagat ggtatgggca atactttgta tattggcagt cttaagagtg aggtctattt
    25021 ctgtttgtac gaaaaagact atgagcaata tatcaagtta gggattcctt tagaccagac
    25081 cgaaacgaaa aatcgttttg aaatccgact gaaaaatgat cgagcttatc atgcaattca
    25141 agatttgtta aaaggtcgca gtattgaaag taccacattt tccattatta accggtactt
    25201 gcgatttgcg gataaggtgg aaggcaaaag gcgaactaac tggcccttga atgaacaatg
    25261 ggggcgcttc attggacgga atcagaagga aattcaatta acttctgaac cgaagcctta
    25321 taccattgaa cgaacgttga actggcttgg acgacaagtc gcccctacgt ggaagatggc
    25381 caaggagttg gatcggttga atcagacaac gtacattcag gatatggtaa ataatactcg
    25441 actatcagac cggcacaaaa agattttgga gcaacaaagt atggcaattg aaaatttgat
    25501 tatatgaaaa gaggaatcgt gatggaaatt gtgaaagata ttttattggt gtttggtggc
    25561 agtatgcttg gtgttaccgt gatgtgcttg atgcatgcaa gtgcagcggc ggaccgagca
    25621 atggaaaaga aaggaaagtg atgaaatgaa tttcggtcaa aatttgtatc aatggttttt
    25681 aaccaatgct caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt
    25741 taaacgcgag ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct
    25801 ggtctttaat gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg
    25861 tgcctgaggg ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata
    25921 caatgaaggt gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa
    25981 agaacggatt ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc
    26041 ttttgagatt gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga
    26101 agagcttaaa ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa
    26161 ttctttcgag gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga
    26221 catgagtgat gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga
    26281 acacttgaag acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa
    26341 ttatttggtt acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc
    26401 agaaatttct atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt
    26461 gataggggtg ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc
    26521 aactgcgagt cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg
    26581 tttaaacaaa ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga
    26641 tgaatataag aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac
    26701 gtgtggagaa aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga
    26761 tatctctgat ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc
    26821 ttgctcaatg aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt
    26881 tattcctgca catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg
    26941 aaagatattt gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga
    27001 aaatcgctca caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc
    27061 cagatatgat ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga
    27121 ggcagaaaaa aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa
    27181 ttcaaaacat ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga
    27241 acctgacttg ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga
    27301 tttttctaat ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg
    27361 gaccactctt agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat
    27421 tggaggaaga ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa
    27481 ggaagcagaa cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat
    27541 tgaacataat ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta
    27601 taaggggaca aaatatcaaa taataagaac acaggataga cattctattt atgaatttac
    27661 acaagagaat ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt
    27721 gagtatatac tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga
    27781 attactagat ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg
    27841 tgtaaacgaa ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga
    27901 taaggcgaag gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca
    27961 taagagttat aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact
    28021 gaaacgtgat gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa
    28081 aattgacttt ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt
    28141 gattaagaat ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga
    28201 tgaaattgat agttatagag cagagctgaa taaatcagat tggaaagtta actataatga
    28261 ggtttcaaag aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa
    28321 ttaaaaatgt tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga
    28381 ttggaagaaa ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca
    28441 gcaatgtcaa gaatgaataa atcatatcaa aatttctatt ctgagagaag tagttcctcg
    28501 cgcgaaattt tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc
    28561 aataattatg ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag
    28621 tttggatctc ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat
    28681 gacatacttg atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc
    28741 aattctataa gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat
    28801 gatttagtag ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt
    28861 gctggtcaaa agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc
    28921 atattggatc agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga
    28981 agtatcatta caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc
    29041 ccagttaatg gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct
    29101 gatcagtgcg ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa
    29161 ggtggcgagg atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa
    29221 taacactaga gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa
    29281 aataaaaagc tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt
    29341 tcagttgcct tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt
    29401 tgtcatcgta tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata
    29461 cttaggcata ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa
    29521 accactggga tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc
    29581 aggaaagaaa gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag
    29641 tgtgaactat gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg
    29701 agtgttttgc ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga
    29761 aatttcaagt gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc
    29821 atgcgttaca aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag
    29881 aaattaccgg tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat
    29941 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa
    30001 ttgcgacaga tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc
    30061 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
    30121 aagaaattcg ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat
    30181 ttaaagtcag gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg
    30241 aaaaagggat accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag
    30301 aaacactggt gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac
    30361 caaggtgctt acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta
    30421 atacaattgt gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac
    30481 aatttacatt cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt
    30541 tagctaccgt tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat
    30601 ctgacaatga aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa
    30661 ccaccttgga tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg
    30721 ccgaatcagt ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata
    30781 ccaatgtaaa actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac
    30841 ccttaagtaa acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg
    30901 ggcttggttt tggggctact cagatgctag gagaattaga agggatttac tttggctaca
    30961 atgtggatac aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg
    31021 gttcagtaac caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct
    31081 ttagtaacaa tctcttagtc tactatgctg ttttgttcgg tgg
//
LOCUS       DACEKH010000023        30755 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEKH010000023 DACEKH010000000
VERSION     DACEKH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.023"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(48..1112)
                     /locus_tag="IW662_001001"
     CDS             complement(48..1112)
                     /locus_tag="IW662_001001"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ0865842.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(1109..2194)
                     /locus_tag="IW662_001002"
     CDS             complement(1109..2194)
                     /locus_tag="IW662_001002"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ0865843.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(2207..3184)
                     /gene="mvaD"
                     /locus_tag="IW662_001003"
     CDS             complement(2207..3184)
                     /gene="mvaD"
                     /locus_tag="IW662_001003"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ0865844.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(3177..4121)
                     /gene="mvk"
                     /locus_tag="IW662_001004"
     CDS             complement(3177..4121)
                     /gene="mvk"
                     /locus_tag="IW662_001004"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ0865845.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            4443..5096
                     /locus_tag="IW662_001005"
     CDS             4443..5096
                     /locus_tag="IW662_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0865846.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            5181..6086
                     /locus_tag="IW662_001006"
     CDS             5181..6086
                     /locus_tag="IW662_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0865847.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(6088..6720)
                     /locus_tag="IW662_001007"
     CDS             complement(6088..6720)
                     /locus_tag="IW662_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0865848.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            7040..7564
                     /gene="infC"
                     /locus_tag="IW662_001008"
     CDS             7040..7564
                     /gene="infC"
                     /locus_tag="IW662_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ0865849.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            7636..7836
                     /gene="rpmI"
                     /locus_tag="IW662_001009"
     CDS             7636..7836
                     /gene="rpmI"
                     /locus_tag="IW662_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ0865850.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            7889..8248
                     /gene="rplT"
                     /locus_tag="IW662_001010"
     CDS             7889..8248
                     /gene="rplT"
                     /locus_tag="IW662_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ0865851.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            8600..9838
                     /locus_tag="IW662_001011"
     CDS             8600..9838
                     /locus_tag="IW662_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0865852.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            9886..12015
                     /locus_tag="IW662_001012"
     CDS             9886..12015
                     /locus_tag="IW662_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ0865853.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            11990..12679
                     /locus_tag="IW662_001013"
     CDS             11990..12679
                     /locus_tag="IW662_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865854.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(13087..13773)
                     /locus_tag="IW662_001014"
     CDS             complement(13087..13773)
                     /locus_tag="IW662_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0865855.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13909..14103)
                     /locus_tag="IW662_001015"
     CDS             complement(13909..14103)
                     /locus_tag="IW662_001015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865856.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(14153..14593)
                     /locus_tag="IW662_001016"
     CDS             complement(14153..14593)
                     /locus_tag="IW662_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ0865857.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(14597..15442)
                     /locus_tag="IW662_001017"
     CDS             complement(14597..15442)
                     /locus_tag="IW662_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ0865858.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            15591..17009
                     /gene="pepV"
                     /locus_tag="IW662_001018"
     CDS             15591..17009
                     /gene="pepV"
                     /locus_tag="IW662_001018"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ0865859.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(17085..18287)
                     /locus_tag="IW662_001019"
     CDS             complement(17085..18287)
                     /locus_tag="IW662_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865860.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(18274..20061)
                     /locus_tag="IW662_001020"
     CDS             complement(18274..20061)
                     /locus_tag="IW662_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865861.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(20074..21027)
                     /locus_tag="IW662_001021"
     CDS             complement(20074..21027)
                     /locus_tag="IW662_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0865862.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(21212..21562)
                     /locus_tag="IW662_001022"
     CDS             complement(21212..21562)
                     /locus_tag="IW662_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0865863.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            21762..22841
                     /gene="pepA"
                     /locus_tag="IW662_001023"
     CDS             21762..22841
                     /gene="pepA"
                     /locus_tag="IW662_001023"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ0865864.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            22984..23304
                     /locus_tag="IW662_001024"
     CDS             22984..23304
                     /locus_tag="IW662_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0865865.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            23326..23790
                     /locus_tag="IW662_001025"
     CDS             23326..23790
                     /locus_tag="IW662_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0865866.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            23986..24591
                     /locus_tag="IW662_001026"
     CDS             23986..24591
                     /locus_tag="IW662_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ0865867.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(24630..25919)
                     /locus_tag="IW662_001027"
     CDS             complement(24630..25919)
                     /locus_tag="IW662_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0865868.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            26347..26826
                     /gene="rlmH"
                     /locus_tag="IW662_001028"
     CDS             26347..26826
                     /gene="rlmH"
                     /locus_tag="IW662_001028"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ0865869.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            27179..27493
                     /locus_tag="IW662_001029"
     CDS             27179..27493
                     /locus_tag="IW662_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0865870.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            27509..27895
                     /locus_tag="IW662_001030"
     CDS             27509..27895
                     /locus_tag="IW662_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0865871.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            27924..29309
                     /locus_tag="IW662_001031"
     CDS             27924..29309
                     /locus_tag="IW662_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0865872.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            29312..29465
                     /locus_tag="IW662_001032"
                     /pseudo
     CDS             29312..29465
                     /locus_tag="IW662_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            29488..30693
                     /locus_tag="IW662_001033"
     CDS             29488..30693
                     /locus_tag="IW662_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0865873.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         9742 a         5771 c         6256 g         8986 t
ORIGIN      
        1 tgcttccgcc gtttatacgt gtttagagcg tgggataaaa gtgttcttta cttttgtccc
       61 acgctctttt ctgtgcgtct tccatacttc actaagtcga tccctcttgc ttcgcaccaa
      121 tccttcactg gtccggaaaa gatcagtgat tgttggtgta gggccgcggt attcgttgcg
      181 ccgaccatag tataaagcag acgaagctct tcttgccatt gcttcatcag tataatagtt
      241 tcttctacgc cgtgattcat cagatgcgtc agtacggttc ctgaagtacc cactgcattt
      301 gcgccgagac aaagggcttt aaatatgtca taggcattgc ggatacctcc ggaggctagg
      361 atttccatag aagtgtctgc ttcatttgct tcaagcaatg aagagactgt cgattgcccc
      421 cagtcagcta aataactcag ttcccgcttg gagcgacggg cgttttcgat ctgggtgaag
      481 cttgttccgc ttcgaccgct gatatcaact gtatgaactc ctaaagcagc tagatcattg
      541 agcgtttctc ttgtcatgcc aaaaccgact tcttttacga tgagggggac gtcaatggct
      601 gtttgagtct cttgaataag tactttccaa ttggtaaaat cacggtctcc ctcaggcata
      661 accaactctt gaggtgcatt caaatgaatc tgtaaggcat ctgcgtgaaa caatcggata
      721 gcttcttggg ctctttcgac agaagtccct gcaccgatgt tggcaatgat tttcccgtga
      781 ggatattctt gtcgcataat cgtataagta tcggctaaag aagggtcttt caaggcagcg
      841 ctgactgaac cagtggcaat catcaaatct gcttctcttg cgacgattgc aagatcttgg
      901 ttgatttttt ttgttttttc gcttccgcca gtcatcgcat tgatataaaa tgggctgctt
      961 agtgtaaacc caactgcctg tgtacttaga tcgacatcag aaactgctat ttgcggcaaa
     1021 gggttatgga tgatccgtac gaagtcaaat tcgttttttt gtttatcatg aaaggcttta
     1081 gctagtgaaa catgttcgtc ttttcgattc atcggttttc ctttctttga tcgctataga
     1141 catgtaacgg cagtggagtg atttctgctt tttcccatgc actcattaaa ggaaggatgc
     1201 cagatttctg gtcaacaatt acgattccgc aatcgccacc acctgcacca gaagattttg
     1261 cggctccttc atactgttca gctaaattac acaatttgtt caaagcaggc gtttcgatga
     1321 cgaccccagt gattgcagat aaatcatgca gtaattgtcg gttttttcga atcatctgtt
     1381 ggatcaacgt tacattattt tctttaaacc ctttgatcat ttcgttgaca cattctgtac
     1441 tgttttttaa gaaaagctga taagccacca ttttgtcttc tctcgaacgg tgaacttgat
     1501 cgaccaaatc agaagtagag gcagggctac ccgtccaacc aatcaataaa cgtaaatctt
     1561 caggagcaat caatggctca atggatagac ctggccaatc cagggcaagt aactcactga
     1621 tagaatgctg agtttcttgt tcttggagcc aaggatggtc gaaggttgag aaagcgatcc
     1681 agccaccata acagctagca gcgatgtcgc cgcaggaacc attatcttga actgctaaat
     1741 tggctagtgc tgcaatcttg aaaatctcca attgagataa attcaacgcg taaaaaacat
     1801 tcaaggcttt gaccgttgca acggtcacgg caccgctaga acccaatcca tatttccggc
     1861 catttgaact gtctaactca cttgttactt tcagatcata aaaggataaa agaatgtttt
     1921 tttcttgtgc atacttttca gtcaaacgaa tagcagcaag gatataatga aaaggatttt
     1981 cccgaatatc taaaactaat tctccgttgc gtcttgtcca acgtacaggc atcccactat
     2041 attgtgcaga ttggatactt ccgacttttc gtgcggattc tacagtgact gttacgaatt
     2101 gatcgactgc agcgataact gctggatgtc ctgtttcaac aactgcatat tctccggcaa
     2161 tatagagttt gcctggtgca gatacttcaa tcataaaaat ctccctttat tcaataatcg
     2221 caattcctgg accagcatgg gcagtaatta cttgttcttt gctaaataat ttggtaaatg
     2281 tttcttgtac ctcagataaa tggctgtttt ccactaaaac tttgacatta gggccagcat
     2341 ccatcgtaaa gtagcatgga atgccttttt ttctcaattg ccgcaccgca tccatcgctt
     2401 ttaagctgtc aggagaccag taagtgaatg gaggctgggc agctaaagtc gttccatgca
     2461 tttttagtcc gtttctttcc atcgtttctc cgagaagttg gaaatctttt gttttgattg
     2521 cttgtttcaa ttgatataga tccccttcaa cggaatctaa ccagccttga taaaaactgg
     2581 atgtttcgac tgttcgacgc atcccatttc tgctggacac ttcttttttc tggtcgttga
     2641 tcaaaacgaa aaccattgct aaatcgtctt cgaaagaatc ggaagggact ggcttagcgt
     2701 aagaacttaa gtcgtcatgg cctttttccc attcaacgaa accaccgaaa atacttcggc
     2761 aagctgaacc agacccgcgt cgagcaaatc ttgaaagaga gagatcgtct aatccaagct
     2821 taagagcagt attgcaagct cctgctaaag cagctagccc gctggcagag gaggctaatc
     2881 cagctgcggt aggaacgaaa ttctgactga ttactgaagc ttttagtgag aggccggctt
     2941 ctttacgaaa aaggtcaaga aactgactga ctttttttgt tgctttttcg tcttgtaagg
     3001 tgccatctag ataaaattca tctaccatat agctatcaga aaagatgact tctgtctctg
     3061 tataaaatgc atccaatgtt aacgaaaggc tgttgttcat tggaaggatg agttcttcat
     3121 ttttcttacc ccaatatttg attagagcaa tgttggtata tgcgcgtgct ttgcctttaa
     3181 acataggtat gtactcctaa tccttgtatc caagtttcag cagcacccgc atcttcaagt
     3241 gcttgactga tttcttgggc ttttgttttt gtttgtgcta atgcaatcat acagccaccg
     3301 cgtccaccgc cagttaattt ggcacccaat gccccggtat cttttgctgt ttggatcaat
     3361 agattaagaa aatcattgct gattgtcagc ttttccagaa gtgactgtga ttcatccatt
     3421 gtctgtgcca acgtttctgg tgaattctca atgattgctt gttttgcttg cttcgtcaag
     3481 tatcctaatt tttggatcat ttgtccagtt tcatgaggct gggtttcaaa gaggtgagcg
     3541 acatctttga cggcttctct tgtttgtcct ttgattcctg tatcagcgac aatcaaaaaa
     3601 gcatcgatgt tcaaagaaaa gtagtcaaaa ggatgccctt ttgtaaaata aatcggctgc
     3661 aagctgctag tagcggctgc atcgattcca ctaggattac cgtgggcgat tttttccgaa
     3721 agctggacat tttctaatag tatttcacga gacaaaggaa atgctaagta atcataaaaa
     3781 gcacgagtga ctgctgtggc gactgcagcg cttgatccca ttccacgttc agcaggaatc
     3841 gtgctttcaa tggtcagttg caagtttttt tggatgtcat gctgtttttt taagtgaatg
     3901 aataattttt tgatattttt taatgcatga ggagcttcgg ctaaatttcc tgaatagtag
     3961 gaagaaacca gttgatcgtc cattgcatcc agctcttcaa ggaatgcggt gacttttgtt
     4021 gcatagaaag gaaaggcaat cgctggttct ccataaacaa ccgcgtgctc gcccatcaat
     4081 atgatctttc cgcttgattc tccttggcca tagtttgcca taatctaact ccttctcacg
     4141 taaaagtgat ttttaagcaa aaaataaagc gcccaaaacg agttgcgctg cttcacgaat
     4201 ttttttgtcc actaagggtc tcaaaaaaca ttcctccttt attttactaa aaccatctca
     4261 gaagtaaaac taagaatacg ttttctaaag aaaaaacact tttttcttaa gaattttttg
     4321 actttttttc ttctttgact agaaaaaata caattgacac tactagatat tgggggtata
     4381 tttaaaaaag gttggagtta aacacaatat ttagtgatga agtgagctgg aggtaaatga
     4441 gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc aagtttaaca gaaatgatgg
     4501 ctgaacactt tgatagtcag gcattttatg aatcgattga tgataatgaa gtcttgccct
     4561 tattttatgc aaaccctgaa cagtatgctt ttttgctgca aatttatttt ttgaataaac
     4621 gttttgcaag catcaaacaa gcaatgaaag atgacaataa tgtattggac cgttcgattt
     4681 atgaagattc actgcttttc catctaaatg cagatctagg aagagcaaca gaaacagaag
     4741 ttcgtgtgta tgatgaactg ctggaaaaca tgatggaaga acttccctat gctgcccata
     4801 aaaaacatcc ggatttatta gttcatattc gtgtatcttt tgatacgatg ttagagcgta
     4861 tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga cccaagtcta tacgattatt
     4921 acaaagaatt gaacagacgt tatgatcaat ggtatgaaga atacaaagaa agtccaaaaa
     4981 tccaaatcga tggagaccgc tataattttg tggaagatcc tcaagcaaaa gaagaagtct
     5041 tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac caaagtatcc atttgattta
     5101 cagttgctca aataaagggt aaacctttat ttgagcgact tttttcttta tactggaaac
     5161 aaatgtcagg aggatagagt atgcgggaaa aacactattt taatcaagac cgtaattttt
     5221 ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa cttaaaagaa gaatatggca
     5281 ttagtgacga gatgctgact tattctctag ataaaaatga acgggcgcgc gtagagtacg
     5341 attcttttga tgaagcgtta ctgcttgtat caaatgttcc gcaccagcaa aaagtcgata
     5401 atcattatga aactagtcct atcgctttta ttttaaaaga agacggtttg tttacattta
     5461 ccacgccgaa taccgagtac gtgattcgac taattcgatc gcttttggaa cggatgccag
     5521 acatgagtgt atacagttta ttgtttcgta cattgttttt gatttctgat tccttttttc
     5581 ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa tctgaaatta agagaaaaaa
     5641 caacgaacaa gaatctgctg caactttcag atttagagat cggtttggtt tacttagtta
     5701 caggaacaaa acaaaatgta gtacttttag aacaaatcaa ggcactggct atttatcgca
     5761 agttgtcaga aaaggaaaaa gaagagttgg acgatgcact gatcgaagca cgacaagcag
     5821 ttgaaatgac gaacttagct tcgcagattt tagaccagct ctctggcact tataataatt
     5881 tgctgaataa caacttgaat gacacgatga aatttttgac ggtctggtcg ctgatcttga
     5941 ccgttccaac tattgtgaca ggattcttcg gaatgaatct ccagttaccc tttacccatt
     6001 ccgttttcgg atgggaaatc gccttgatca tcagtttggt cttatctatt tggatgctta
     6061 ttgcactgtg gcggcggatt cgttgatcta gtctttgatc tgcaaaccaa gggcttgggc
     6121 gatcacaagt gcttgctctt gattgacgat tagtcccgcc aaagaatctt gagataaagc
     6181 gattgttgtg aagaaattag tcgtaaagtc taatcccttc agagaagttc gaaaccagtt
     6241 cgaaccggtt aactgattgt cagacaaaaa aaggtttttc caagtgacct cataaaattc
     6301 gctgtcttct aattgacaat gatcgaagga gacagctttc aagtttgtac tactaaagga
     6361 agccatcgaa gcaatacaat cagtgaaagt acagtctcga agataacttt cggcaaaatt
     6421 cgtacctgtt aatttacatt gatgaaacac gacttggtgg aaactgccac caatccactc
     6481 cagattggaa aaatcacatt tttcaaaaat gacattactg cattcaaaat gacgtaaacc
     6541 tgttttttgc atcgtaagtt tttggatatg actctctctt aataccatat ttttggcatc
     6601 gagatagctt tggttacctc cgttgatcag aagaccagag aagatctcct catcttctaa
     6661 ataaaactct ccctttgtaa gaagaggaag agaaggtgga ataggttcga tttttttcat
     6721 gtgggttcac ctcgtttttc tttagtatag caaagaagcg ggaatcactg caagaaagcc
     6781 tgctaatcta atcattttca gaaaaggaag acaaactgta tgaaaaacat tgacaaaaag
     6841 attagttttt gctatactat aatggttgag aaataaaagc agaagcaccc gcttctcgcc
     6901 ttagttgata atgtcactgg gctagatagt tttcactggt tctattaggt gaagtacgcg
     6961 cgggttctgt aagagaactc gtttttttat tgccgtttat tcggtaagct tttctttctc
     7021 tcataatatt tggaggtgaa tgaccatagc aaaggatatg atggttaacg acggcattcg
     7081 tgcacgagag ttacgattga tcggttcaga tggtgagcaa ttaggagtta agacaaaagc
     7141 agaagcattg aatattgcag aacaagcaaa cttggatctt gtgttagttg cccctggtgc
     7201 gaaaccacca gttgcgcgaa tcatggacta cggtaaatac cgtttcgaac aacaaaagaa
     7261 agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta aaagaagttc gcttaagtcc
     7321 tacgatcgac ttgaatgact tcaatacaaa acttcgtaat gcacgtaagt tcttggaaaa
     7381 gggcgataaa gtgaaagctt ctatccgttt caaaggccgt gccattaccc ataaagaaat
     7441 tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg gatattgcaa cagtggaaca
     7501 aaaagcgaaa atggacggac gcagcatgtt cttgacgctg gcaccgaaaa acgacagtaa
     7561 gtaatttgaa tgactaactg gctgttggac agccgatagt cggataacga aagattttga
     7621 ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg gatcagcaaa acgtttcaaa
     7681 cgtactggta aaggcggatt gaaacgcttc cgcgcgttta caagtcaccg tttccacggc
     7741 aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca tggtttctag tggcgacttc
     7801 aaacgtattc gccaacaact agcaaaaatg aaataagaag tacaaataac agaatctgaa
     7861 gatttatttt tattaggagg aattatacat ggcacgtgtt aaaggtggaa cagtaactcg
     7921 taaacgtcgt aaaagagtgc taaaattagc aaaaggttac tacggttcaa aacacacttt
     7981 atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac tacgcatatc gcgatcgtcg
     8041 tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt atcaacgcag cagctcgtat
     8101 gaatggcttg agctactcaa aattaatgca cggtttgaaa gttgctgaaa tcgacatcaa
     8161 ccgtaaaatg ttagctgact tggctgttaa cgatgcagca gcatttactg cattggctga
     8221 ccaagcaaaa gaagctttgg ctaaataagc taaacaatta taaagaggaa agaagataac
     8281 gaaaagttat gttctttcct ctttttttat ttacaatcct ccatcaaatg aaacaggaca
     8341 ataacgtata agtctaaaat aacataatta aggtttttta attgaaaact gaacatttat
     8401 ggtcatcctt taatgaaagc gctaactaca taagttagaa tgagtttgac tgatatatta
     8461 gtttgcttat cagaaaaaca aaatggacgg aggaataaca tgagtgatat tattattggt
     8521 attttgttag ttatctcatt ctttttcatg gtctggtact gtgtaaaagg ctacaacttg
     8581 atggtcggct tttgtcatca tggctactgt atggatggga ttagcgctag tcggaaacac
     8641 gttttcacca aactcggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta
     8701 tacgacgggg ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt
     8761 tttcgggcgt gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga
     8821 acttggaggg gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat
     8881 attcatgtcg atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccgat
     8941 cttgatgtca ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat
     9001 ggctggaatc tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt
     9061 caatccagcg gctgaaagct atacttataa tgactatttc caaattggta tgattgggat
     9121 gattgtaagc ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg
     9181 ctatgcaatg gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt
     9241 agccgtatta ttaccagttt taggggtcgt tcttttagat cttcaaatca tcttaggttt
     9301 tattctagca ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga
     9361 aatctgtcgt cagtttgcga aattatttac agatggcgca gtcgatgttg caccaatggt
     9421 tggtttcttg atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt
     9481 ttcagctatt tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc
     9541 tatattgaca ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc
     9601 tattttagct gtcgtgttag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt
     9661 atttgcaatt acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg
     9721 gggattaggt tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg
     9781 ttggatcatc ggtgcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta
     9841 aacaaaacgt cgataaaaac gattagataa atggaggaaa agaaaatgaa acgttttgtg
     9901 cgaatgggaa ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg
     9961 cacgaaatca tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc
    10021 gcaggtgtcg ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat
    10081 gttgtgtttg ttgcacattc taccacacaa gcgactaatg cactgattga aggagatgta
    10141 gcaaaagtcg gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga
    10201 caaactagat tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca
    10261 ttcttacctg tcaaaaactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa
    10321 agagaaagag cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca
    10381 gaacgagttg tttatgaagc agcatctgtg aaagcgatcc ctacaacgat ggcttctgac
    10441 atcacaaaat tatatggatt gacaagaaga actcggacag cagcaatcaa cgcatctatt
    10501 ttaccaaaaa tgctagatac ggcaacctcg acagaagact ccgtacgaga agcaggggtc
    10561 aatgtttctt tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa
    10621 aaacgacctg ttttaaccat gctttccggt cctgctgcat cagtcatggg ctctttgatg
    10681 tacttgagag cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg
    10741 gtcattaaaa atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat
    10801 atctcttcat tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat
    10861 cagtcaggaa tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca
    10921 gtatttacgg agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa
    10981 ggagaccctg ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca
    11041 aatacatgtg cagccaatgt tcttggatta gtgcaagaag aacatttttc ttatggaaat
    11101 gtcccatctg cacgaaaagc catacaggca ttagcagact attgtcatac gacggttgaa
    11161 gacatcgcag agcagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa
    11221 ttggcagaca agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc
    11281 gctgcctcat tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa
    11341 aacgccgaag tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa
    11401 cgtatcatcc cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat
    11461 aaagcgattg aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca
    11521 catacttcaa aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta
    11581 accaaggaga tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat
    11641 aaaaatgaag tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat
    11701 cgttcaaaaa atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa
    11761 cgtggacatg cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa
    11821 ctttgggaag atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc
    11881 tgcttaggag caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg
    11941 atggatctgg aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat
    12001 atcaaacaaa cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat
    12061 ttggggattc taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca
    12121 gaaaattctt atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa
    12181 aaagtaccct tttgccgatg taggagaaat ctatgaacaa atggctgttc caatcgtttc
    12241 ctgtgaatca gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg
    12301 gatccgattg atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc
    12361 taaagaaaca gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc
    12421 tgttaaggga atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac
    12481 acatcaatca aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat
    12541 gaatcaaagc gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga
    12601 aattcgtagc caagggatct tagaagaaat aaaagaaata gaaaaaagat gtacggagta
    12661 tgatttagat tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg
    12721 aataaacggt gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga
    12781 gaagctgttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
    12841 ttggaaaaag gtgttcaaaa gcttgcatct gcggtaataa gaacagccaa gttaaaaaca
    12901 taagaagcta tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac
    12961 ctctcaataa acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa
    13021 atatgaggaa ctattttgcc gtttatttct ctaatactca ggtcaaaacg ctgattccaa
    13081 aacctcttat ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat
    13141 cacgtattct tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc
    13201 atcaaagtta tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc
    13261 ccagtctaat tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata
    13321 actttctagc caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa
    13381 atcagtatcc aaatcaaaaa aagctttttt attttgagcg atcactgcct gatcggtttg
    13441 ttctagtatt ttttctagtt tagcttgatc ttgcttggtc attttcgcac tgcattcttg
    13501 caggatcggt ttttccaact tttcccgcgt ataacgggct aatgaagcag atcggatatc
    13561 gatttttgaa acataagtgc cggactgtgg aatggttttt acaagctctt gattgcgcag
    13621 ttggatcagt gcttctcgaa tcggtgtacg cccgatagct agttgttctt ctaacacttt
    13681 atcggagatt ttacttccag gtgctaactc ggaataaatg atttgtttac gtatagattc
    13741 ataagcttgc tgttgcaaat tattgataac catttaacct tcctttctat gcagatataa
    13801 tctattataa cataaatcgc ttacaaatcg tgtgggcata aaaacacagt ctgcaaaaaa
    13861 agccctgata ggcatgccta ttagagcctc gaaaagaaaa atattgaatc aatctggtcg
    13921 tacctcttgg atcggtttga cacgaccgcc gattctttta gcaaaagctt cggattcttt
    13981 tttgtctttg aaaatgagca gattgtctaa gtttttttcc cgagtgcgac agccagtttt
    14041 atcgatgtaa ccgtgcatta ctttaacgac atacatgggg atcacctgct tttttctttt
    14101 cattatacga actactcgac acaatacaag aaaaatgctt agacggtatt tattaagaat
    14161 ttacatcatc caaagaagcg tacgcttgga ataactgttc taactccgga ttgactttgc
    14221 tcagcttcac taatcgttgc gaatcagaaa gtaaaaaaca gtgtttacta ctgccagtag
    14281 tttttaattt tttgtcttca gttcccacaa tttcgtagct aaaatccaac cgtgttcccg
    14341 tatacttttg gatagttggg tggatgctca ctttttctcc gtaatggatc atttctttat
    14401 agctgcaatt tacttccaga acggggatga tgatcccaga ttcttcgatt tttttatagg
    14461 gaaaccctag atgtaccaat aaggctaccc gagcctcttc aaaccagcgg atataattgc
    14521 tgtgatgaat aatccccatt tgatctgttt cataataaaa aggttctcgc acataacctg
    14581 taaatttctc catgactcat gcctcgtttt ccattgattt tttacaaaaa taattcatcc
    14641 acttcggaag tgcttcgctg atttgtgtcg gcaaaacaat atattgttct tttgccagct
    14701 ggtctccaat gaaactatga agataaacag ctgctgcaat cgtttggtca ttttgaggaa
    14761 actgggctaa aaatcctgtg atcatcccag ctaaagtgtc acccatgccg cctgttgcca
    14821 tagcagcaga acctaaagga ttggtgtaga agtgctggtg accataaatt tttgtctcat
    14881 gacttttcaa aacgagcaca gcaccgattt cctgttgctt ctttttgttt tctttatccc
    14941 gttgttcttg tatcggtaga gaactcaacc gttgccactc catttgatga ggtgtaagta
    15001 cagtttgttc gggatagggt agcaaaagat gattagcaac aagtaaactt aaagcagagc
    15061 catcaatcac cagccattgt tcttttgatt gtttttctag tacttgtttg aaacgttcca
    15121 aactcttaga agaagtaccc aatccgggac caatcaagag tacatctgct tgctttatta
    15181 cggaaagaaa ctggtcagta tccgtccaat ctacaatcat tgcttctggc agatgactgt
    15241 gaagagcact ccgatttttt tcatcagtta ctacagtcgt gagacccgct ccactgtgta
    15301 cggcagctaa agcactcata atgattgctc cgccatattg ttcattgccg ccaatcaaaa
    15361 cgatacgtcc aaatgttcct ttatgtgaaa tctttggacg aggtgtgatg actcttttca
    15421 ctagttgttc gtttatcagc atcgaaaaac ctccttttac tcattatagc gtatattttt
    15481 tttagagaaa aacagataag aaaaggaatt gtcagatttt ttgaactatt tttttctgta
    15541 atttgtggta tcatgaacac gaagacttaa ataagtggag gattaacttt atgacaattg
    15601 attggcaaaa agaagtagaa gcacgtaaag aagacttgct tgaagacttg aaaaatcttt
    15661 tacgtgtcaa cagcgagcgc gatgattcaa aagtaacacc agatgcacca tttggacctg
    15721 gacctcgcga cgcattgaaa catatgttag cttatggcga acgtgatgga tttaaagtaa
    15781 aaaatgtcga taactatgca gggcatattg atcttggcga aggagacgaa acgctaggga
    15841 tatttggaca catggatgtc gttcctgcag gagatggctg ggacacagat ccttatgaac
    15901 cagtaatcaa ggatggaaag atctttgcac gcggatcaag cgatgacaaa ggtccaagta
    15961 tggcagctta ctatgcgatg aagatcatta aagaacttga cttgaaacta tcaaaaaaag
    16021 ttcgcttcgt agtcggcagc gatgaagaaa gcggctgggg cgatatggct tattatttcg
    16081 aacatgaaga agaaccagat tttggtttct caccagatgc tgaattccca atcatcaatg
    16141 gtgaaaaagg aaatgtttct ttagctttac gcttcaaagg cgacaatgca ggagattatg
    16201 tcttgaaatc ttttgtttcc ggacttcgcg aaaatatggt gccaggaaca gcaactgcag
    16261 cacttcaagt accaagtgca gatgcagcta tcgcgatgga agaagcattt taccaattca
    16321 tcgaagcaaa tccagtaagc ggaacgattg aagcggacaa tacgtatgta aaaatcgaat
    16381 tagttggtaa aggcgcacat ggtgcaagcc cacaatctgg tatcaatgca ggttctttct
    16441 tggcattgtt cttagataac tatgaattcc taggatctgc taaacaattt atccatgtag
    16501 cagctgctta tgtccatgaa gatttttatg gagaaaaact aggagtcgct tatgaagatg
    16561 aaaaaatggg caaattgaca atgaatgctg gactatttgc ttttgaagaa aatggtacag
    16621 aggaagcgaa cttcatcaat atgaatttcc gttttccaaa aggtgtaaca gtagacggat
    16681 tgcaaagtga catcgaacaa acagtggggc aagaaggcgc aacagttact cgaggtgctc
    16741 gcgtaatgga accacattac gtaccaatgg atgatccatt ggtggctaca ttattgcaag
    16801 tctatgaaga ccacactgga gaaaaaggct acgaacaaat catcggtggc ggaacttacg
    16861 gacgtttgtt gaaacgtggc gttgcttatg gagcgatgtt cccaggatat actgatacaa
    16921 tgcatcaagc aaacgaattt atgagtcttg atgacttgtt cagagcaaca gcaatctatg
    16981 cagatgccat ctatcgtttg gcgaaataaa aatactaaaa aactccggct atctctgaca
    17041 gctggagttt ttatgtttaa aaatgaatta gtacatcggt attcttaaaa gtaatttagt
    17101 tcgtcttttg atttctcagg agggatcaaa aaggcgaaag cactaagtat agcaaaactt
    17161 gtgatgattt gcgtacgata cgtaaaccaa taatgaatct gtgagtgatt agctaaaata
    17221 ttgtaccaaa tatacggcgt tacagcagct aaaacaaaca aaactagaag ttggaacgga
    17281 cgccagctat ttcgtttacg ataagcaaaa tagagaaaaa caagacaagt aatcaatagc
    17341 aataatattg ctacttttgg atacatcaat ctaatattgc ttttcagcat ctctaatcga
    17401 tgcaacgggt atttgtcgtt tccttccgtt cgaaagagga tttggttgat tgcatctttt
    17461 acaatgtttt tgttcaatat gatcgatgat aggatccatt tagccaccca tgtcaaacca
    17521 tagcctaatc cccaaaagat accggtaaaa acaaaactgc ctacttgttt gctcaatgaa
    17581 ctgcgtttat ccattaaata cttatttgtt acataataaa taatgatcac aggtatcccc
    17641 caagagatga ccggtgttgt caacaagtca aaaaagttag ctaaagaacc aatgacaaag
    17701 aaatacaaga ataattgttg gttggtgttg gagagttgtg gttttgttaa aaatagtaat
    17761 acagacaata aagtcaaaat ccaaatattt gaaaattgca tactgaagaa aaaggttgca
    17821 gcatttccta tagcaagcga aatcaacaag gctgtcgtta aatagatgtc gctacacttg
    17881 gttaaaaagt agctcgtcaa tccaatcagc agaaaaagaa tggctgcgta aatcattcga
    17941 atactcccca aactcatccc tagcagtaaa ggtcgtaaaa aaagctgata accatgccaa
    18001 taacggggat aatccgccat agacatcgcg ttctccaagg agttcaattc aggatctgct
    18061 tttgtttttc gtatcatcaa atgatcagta aaattatcta accgagtgcc tagcatattt
    18121 ccttgattga tagaaggata cattccttct ttttcaacca tctgtagaga tttttctgca
    18181 ttcgattcaa tagagtgctg cggaatgaaa aaagtaattg tcagtagtac gcaatagcta
    18241 gcgatcaaga gaacaaaagc aaaaaatatc tttttaaata gtttcatgag tatctcctat
    18301 atctgttttc attttttctc ttttatctag taaaaaagga gagaggatat aaacaggtag
    18361 acctaatgct aaaacaaaca cataccgtaa actaaaagcc acaggagtag caatcataat
    18421 agtagcaaaa acaagcatac cgggaagaaa aaggagtaaa taccgatatt ttttcttaaa
    18481 gattgctaaa agtgcactgc cgatagctaa ccacagtaat gtgccggaac caaagaaatc
    18541 acgagcatcg atgatttttt tcattcctga caatccagta agatgttcaa tcaaatctac
    18601 ttgcttcata ccataattat tcgtgtttac acgagtatct aaaaagccgt aactattttt
    18661 tgtaccaggt acccagaacc cgaacgtttc taaaatataa gcatctgtat agatttttat
    18721 attacttggt aaatttcttg cccatagtga tagaaattct tttttatgtg tattcaaaaa
    18781 ctcattgtta aaatttttat gccacttgat tgagtctgca agactaggag aatacgcttc
    18841 ataatctttt atcgggagta gctgattcat gaatgcttta tctttcttag acaagtcacc
    18901 atccataaca atagttctag aaatttgctg caaaggaaca cctaattgtt cagcagtttc
    18961 tgtaggaata gcataacgag ccatcaacgg gtggatgagt agtccagcaa tcactgtata
    19021 acccagcacc acttgaaaga atgcttgata ccttttttgt ttcaaaataa tcaagccgca
    19081 aatcaaacct gtgccggtta ctacataaat cccattattt cttagatagg tggtcagtaa
    19141 agcaaggatg attacgtaaa tctgattgag aatcgttggc ttttctcgtt gaaaaataaa
    19201 gtcaaaaacc gctaaggaaa ataaaaagca agcgatagag aacaaagtat ctttttgcat
    19261 gttcaaggcg tagattggaa aaaccggtgc aaatgcaaaa tagcataaac taatgagtac
    19321 cggaaaaaaa tggatgtttc tttttaacag ccagcaaaga gaataactta tcgctgaagc
    19381 tgttatcaat gtttgaaaaa tcgaaaataa aaatacaccg tgattgatcg tatcagttgg
    19441 taaatcatga acaaatagct tgaccagtaa ggtgaataag actggatgat gattgcttat
    19501 aggaacatca cccatagatt gggcgaccga acttaacgaa tcaggcaaaa ccactccagg
    19561 atacaatgcc aacagataag gaagccaaca cagaactaaa aagacagtaa agaataaaaa
    19621 atattttctt gaagagaaag cctgtgtgtt ttgagcagta aaaaatgttt ccagttttgg
    19681 cattgataaa taaatcgatt gtaaaaataa aaaagaatac acaaaaatgg agaaataaac
    19741 aagaaagtaa aaaatacttc catcagcaat cgttgtcttt gtatagtttg ctgagacata
    19801 ctgaaattgt atctgtctgc caatagaaag tgtaagagca aaagctgcag aaaaaaacaa
    19861 tcgggcaata gctgcaactt tgttttttat tgaaaacaac tctattccat attgcttaaa
    19921 agcaagtagc aaaaccatgg ctaaaccaat gattactgtt ggattcaaag ttcctagacc
    19981 ataagaagta aaaaaagcaa aacctgacgc aaataaagtt gcaaaaaaag taggaaatag
    20041 attctccttt ttcttatgca taaaaaaggc ctcctacttc atttctttat tttgatattc
    20101 gtggtagaca tggtataaat tcaattcata ttctttcttg gcatttgtga cgaccgtatc
    20161 caaaatcaac cctgttacta atgaaagaag ggctaaagtc atcaatccag tacttaaaat
    20221 cgcagaaggg atgcgtgtaa taaagcttgt cagcatatat tcacgaatca caggaatccc
    20281 catgaccaat ccaaataaaa agaagaagat ggtccagatc ccaaagaaaa gcaaaggttt
    20341 ataatctttg cacattttga tgatcatcat gatcactttg aatccgtcag agaatgtatt
    20401 caattttgat tcgctacctt caggacgatc gcgataatcg attggaatct caacgaactt
    20461 gaatttttta tctaaagcat gaatcgtcaa ttctgtttcg atttgaaaac cagaactcat
    20521 aatagggaag cttttgacaa aaattcgatt gaatcctcga tagccggtca tgacatcgcg
    20581 aatttttgtt ttatataagt aagtaattgt atttttaacc agattattcc caaaatcatg
    20641 gaaaggacgc ttattttcat caaaataggt tccatttgat agacgatcgc caatgaccat
    20701 atctgcttcg ccagaacgta atttctcaag caaaccatgt gcagcttctg caggataagt
    20761 atcatcacca tctaccatca aataataatc ggcatcaata tcaaaaaaca tttgacggat
    20821 tacattccct tttccttgac gtggttcttt ctttacgatt gctccgcctt gaacggccaa
    20881 ttcgtatgtt cgatcggtcg aattattatc atagacataa atatctgctt ctggtaattc
    20941 ccttttaaaa tcttcaataa cagttgaaat tgtcgcctct tcgttataac aaggaatgag
    21001 cacggcaatt ttttcacgtg attccatttg tcactctcct tatataataa aactaatctt
    21061 atttcaataa taccgtataa taactacaaa tactagctat tccaagagga aatagcccta
    21121 tttttataaa aatcacccac tataaaagaa ggataggata cctctctttt agatagtggg
    21181 tgcgtattcg ttatttcatt tttttgacgt attaatctat tgcgcccttt tttcttgaaa
    21241 tgtctaatac ggttcctgta tgagcatctg ctaaaaattc ataataaaca gtttcgccat
    21301 cttctatccg ggaaagccca ccccgataag cactcgaccg aacggcgaat ttccgaatag
    21361 gttctttttc aaaagagatc caagcacctt cgattgcgcc ttcttttaaa aaagatgttt
    21421 tgatattttc aagcacttgg tctgctgata acactttttt gcgatgatac caaatcgttg
    21481 aagctacccc actcaataat cctagaccga tcccaacggc taacccgcct ttaaaatagc
    21541 ttaattcgct ttcttcatac atgtggatat acctccttct tctcacttgc tactctttgt
    21601 gtttatttta ccacactctc atattttctt tgaaataaat aaccgctacc agaaaaaata
    21661 agctataata aatacgatcg gtctgcagaa aaaatcatat tttatccgca gatcaatata
    21721 agtctgagag gagtttagtc tgaaatttag acgaatagca tatggaagaa aaaacatttc
    21781 aacgaatcaa agagttaaca gaattacaag gaacaagcgg ttttgaacaa gacgttcgcg
    21841 cctatatgga aagtcatatg gaaccgctgg tagatgagtt acagttagat ggtttgggcg
    21901 gcatttttgg gctgcgccac catgaagaag cggatgcacc tcgtgtaatg gtcgcagcac
    21961 atatggatga agtcggattc atgctgacac aaatccaaga aaacgggctt tttaaagtcg
    22021 tacctctagg cggatggaac ccttacgttg tttctgctca acgttttaca cttaaaacct
    22081 ctactggtaa gaattatcca tgtatttctt cttccgttcc cccacattta ttacgaggaa
    22141 caagcggaca aaaatcagta gaagtaacag atattttgtt cgatgctggg tttgaatcaa
    22201 gagaagaggc aatgagttac ggtgttcttc caggagacac aatcgttcct tacgctgaaa
    22261 cgatcaaaac agccaacggg aagaatatca tcagcaaatc gtgggacaac cgctatggct
    22321 gtacaatggt attagaagct ttagaagcgt tacaaaatga aacattaggt catacattga
    22381 ttgcaggcgc gaatgtccaa gaagaagtag gcttgcgtgg ttctaaagct tctgtcaata
    22441 aattcaagcc agatctgttc tttgcagtag actgctctgc agcagatgac acagtgacga
    22501 aaaatggcac attcggacat ttaggagaag gtacattgat gcgtatccaa gatccaggtt
    22561 tgatcatgct tcctcgtttg cgtgagtact tgctagatat cgctgaaaca aacaacatcc
    22621 cttatcaata ctttgtttca aaaggcggta cagatgcagg tgctgctcat acacaaaatg
    22681 aaggaatccc aagtacagtg attggcgtag tgggacgtta tatccatacg catcaaacga
    22741 tgttcagtat tcgcgatttt gaagctgcac gagaaatgtt gattcaaaca ttgaaaggtt
    22801 tagataaatc aacagtgaac acgatcgtat acgggaaata acgcgatagt gaaactataa
    22861 aatctggcaa aagcaacctt ttgtcagatt ttttttataa aaagaggatc aatgtttgtt
    22921 agttcgaaag aaaggtgtat aatttaattg aaaaatacat aaacagaaag tagaggaaaa
    22981 agaatgatta tcccaaatag tttagaagaa ttagcaggct acgtagaaac tggaaaaagt
    23041 gtatttttct ttactgccgg ctggtgcgga gactgccgtt ttatcaaacc gcaaatgcct
    23101 gaaatcgaaa atgacttttc tgattggcaa tttattgaag tagaccgtga caagtacata
    23161 gatgtagccg ctgaatggaa catttttggt atcccaagtt ttgtcgtgat ccaagacgga
    23221 aaagaattag gtcgtctagt gaataaagat cgtaaaacaa aacaggaaat cgaatcgttc
    23281 ctagaaagtg taataaaagg ctgacatctg ttaggagggg tcaagatgaa tcaagaatat
    23341 aagaacattt tagtaggtat tgacggtagt gaacaagcac ttgctgcttt caaaaaagcg
    23401 ttagaagtgg ctcgccgtaa taatgggaaa gtatatgtag caaacgtaat cgatcagcaa
    23461 ttttataatt ttatgggcta tgctcctatt gatcaaaatc tagtcgatca gcaaactgaa
    23521 acagccaagc aaatgatcga agaatgtaaa gaatatggaa aatctgttga ttacacaaat
    23581 atagaaggaa ttattgcata cggctctgtt cgtgaatcca tggcagtcaa attgccaaaa
    23641 aaatatgcaa tcgatctaat catggttggg cagtctggac taaatgcagt cgaacgcttt
    23701 atgacaggaa gtgtagccag ctacgtcata cgtcattcac cttgtgatgt cttgatcgtt
    23761 tctgacgaag aagaacagcc aagtaattaa gcaagtggaa cttgtataac ctttttaatc
    23821 aaacagtaaa gggactgtga caaaagtctt gtcacagtcc cttgttacga ataaatggtg
    23881 ttccagaaga agcttcttcg gaaataaagc atgaaaaaaa tctcatgcag tgaatgcaat
    23941 tttttgttaa gataaatctc agcaaaggat aatggaggaa agaacatgat ttttgcttat
    24001 aacaaagcac acgtcggaga cactttgatg gttatcatag ccgacgataa aggaacagaa
    24061 aacactgtcg aacgtaaatg gaatgttgct cggatcaaag atgaacaagg tcaagttgtt
    24121 gcttggaact ttttccatat ctccgatcat ctaacaattg aagggaacgg ccaagtaagc
    24181 ttaacagatg aagaattagc gaaattgaat cgattgatta aagaagcagg attcgaagaa
    24241 cagcttgaag ctgatcatga accgaagatc gttgtcgggt atgtaaaaac atgcgtcccg
    24301 catcctgact ctgaccattt atcgattact gagacagaag tggacaatgg gacagtattg
    24361 caaattgttt gcggtgcacc aaatatcgaa gctggacaaa aagttgtcgt tgcaaaacca
    24421 ggagccatga tgccagatgg aatgatgatc tggccaggaa ctttgcgagg ggtagccagc
    24481 tatggaatga tttgttctgc tcgtgaattg catttgccaa atgctccaga aaaaaaagga
    24541 atcttagtat tgcctgagga tgccgtagtc ggagaagtat ttccaaaata aagaggttgt
    24601 gacaagtctt tgtcacaacc tctttattat taattttcac tattttgatt ttgttttaat
    24661 gccgattgat cgatcgtcaa ttcaacagta gttgtttttt ctttcgagcc ttcatagtaa
    24721 gtgattttga cagaatctcc cacttttttc ttatacaaag cagattgaag ttctacacca
    24781 gaagaaactt ctttgtcatc gatcttagta atgacgtcgt attgtttcaa cccagctttt
    24841 tctgccggtg tagctgtttg gacatttgtc acgatgacac cgttagttac agatgaagga
    24901 atcttcaaga tttgttcttg ttgctgagta gaaacggcag ataagtcgac cattgtgata
    24961 cctagagctg gacgtgtcac ttttccgtct ttttccagtt ggttgatgat attcactaca
    25021 tcgttacttg ggatggcaaa tcccattcct tctacgctta cattagaagt cgattcagaa
    25081 gtacttgcga tcttacttga gttgataccg ataacttgtc cttcgatatt cactaaagga
    25141 ccaccagaat ttcccgggtt gatcgctgca tctgtttgga tcgcattgat attgactgtt
    25201 tcgttcgatt cattcgtact tgttacttgt cggttcaagg aagaaatgat tcctgaagtg
    25261 acagaattcg catattcaga tcctaatggg gaaccaatcg caatcgcagg ttcaccaacc
    25321 ttcaaagcac ttgagtcccc aaaagaggca accgtttcga ctttgtctga attgatcttc
    25381 aacaccgcta aatctgaata ggcatctgtt ccaactaatt cagcttttac cttcgtaccg
    25441 tctttcatca tgacttccaa accttgctgt ccatctacta cgtgattgtt tgtgactaca
    25501 taagcagtat tcccgtcttt tttgtagatt acgccgcttc cttcactata agcttccaaa
    25561 ttactatcat ctgagctgct actttcttct tgctgcccga ataatccgcc aaaaccgctt
    25621 gattggtttt gactttgtaa gttgataaca gaaacgacag cgtcttgaac tttatctacc
    25681 gctttggtga tatcagaatc tacattgact ttgacatttt ccacaactgt ttcaccggca
    25741 gaattttgat tgccgcttgt agcagtagaa ctattgttgc cagtgcccat cgctgcataa
    25801 aaaatgccag ctgtcagtag tcctccaacg atccctccaa caagccccag acccaactta
    25861 cgccagaggc tattattttt attttttttc atctttggtg tcacattttt tctatccatg
    25921 tgttcctcct ccaattacat tcatttatct actgttagta tagtcttatt tgttgacttt
    25981 agtctacttt gagactttga actttttatg aaaaacttta ggaaaaatca ttgttttcag
    26041 atgaaaatat ttgattttct ttttccacag atattgtgta aaactacgcg gataggattg
    26101 gaaaaataag ggataaaaca tatccacagc ctgtgtataa agtggataaa ttcttataaa
    26161 acgtatgttt gcctaatgaa tgtgatgaaa taaggtaaaa caacgctgtt ttctgtggat
    26221 aaacctgtgg atgttgtgca taactgttgg ggaaatcttt ttaacaaaaa tagtcaaaat
    26281 aaacatatag acgaaaaaat tgattataat aaacaaagtg agcgtgaaga tcaaaggaga
    26341 gaaaacatga atatcaaaat tatttcagta ggaaaattaa aagaaaaata cttgatccaa
    26401 ggtatcaatg agtatgtcaa acggctgaat gcctatgcga aaatcgaatt gatcgaagtt
    26461 cctgacgaaa aagccccgga gaatttaagc gaagcgcaaa tgagacaagt aaaagaaaaa
    26521 gaaggagaac ggatcttagc gaaaatcaaa gaacaagaat acgtctatgg cttagcaatt
    26581 gaagggaaaa atcccacaag cgaatctttt gctaaacaaa tcgaccaatt aggtatccaa
    26641 ggaaaaagcc accttgtttt tgttatcggc ggttctttag gactcagcga agccgtcatg
    26701 aaaagaagca atgctcagat ctcttttggc aaaatgacgt atccacatca attgatgcgc
    26761 ctgatcctcg tcgagcaaat ctaccgtgcc ttccggatca acgcgggggc tccataccac
    26821 aaataatgat attttttaag agaaaaatag cataaaaatc tagttatccg cataaaaact
    26881 ggacttatca cactttatca aggtcaaaac cactcaattt actactaatt tactacttat
    26941 gaatgagctt tgatacgacg atttatcctt gaaaagtgaa gatataaaga tacttccaat
    27001 aaaatttgaa tatttaatag gtagacactt caaaaaatga ggtgtctatt tttttacccg
    27061 attttgaaag gaagtgaact tatgaaaaca aaaaatcaag aatcaaaagg tcgttcccca
    27121 ctctttaaga ccatcaaaca ttcattcagc caataaaaaa gaaaggatag gtaaaaatat
    27181 ggaacttaaa tttgtgattc ccaacatgga aaaaacattc ggcaatttag aatttgctgg
    27241 cgaggataaa gtcgttcagc gaagaatcaa cggacggcta actgtcttat caagaagcta
    27301 taatctctat tctgatgttc aaagagcaga tgatattgtg gtggtgcttc ctgctgaagc
    27361 tggcgaaaaa catttcggct ttgaggaacg tgtgaagtta gtcaatccac gtattaccgc
    27421 agagggctac aaaatcggca ctcgtggttt tacaaattac cttttacatg ctgacgacat
    27481 gataaaagaa taaagaaaga gaggaaaaat gatgagatta gcaaatggca ttgtattaga
    27541 taaagacacg acttttggag aattgaaatt ctctgctcta cgtcgtgaag tgagaatcca
    27601 aaatgaagac gggtcggttt cagatgaaat caaggaacgt acctatgact taaaatccaa
    27661 aggacaagga cgcatgattc aagtaagtat tcctgccagc gtgcctttga aagagtttga
    27721 ttataacgca cgggtggaac ttatcaatcc cattgcggac accgttgcta ctgccaccta
    27781 tcaaggagca gatgttgact ggtatatcaa ggcagacgat attgtgctga caaaggattc
    27841 tagttcattc aaagctcaac cacaagcaaa gaaagaaccg acacaagaca aatagtcgct
    27901 aggtagaaag gagacttttt cgcatgaaac agcgtggtaa aaggattcgc ccatctggta
    27961 aagatttagt ctttcatttt acgatagcgt cactcctgcc tgttttcctg ctggttgtcg
    28021 gactgtttca tgtgaagaca atccagcaga tcaactggca ggattttaac ctatcacaag
    28081 cagataagat tgacattccc tatttaatta tcagtttcag tgtcgcaatt cttatctgct
    28141 tgctggtagc gtttgtattc aaacgggttc gctatgatac ggttaaacaa ctttaccacc
    28201 gtcaaaaact ggcaaagatg atacttgaaa acaagtggta tgaatctgaa caggtcaaaa
    28261 cagagggttt ctttaaagat agtgctggtc gtacaaagga aaagataacc tacttcccta
    28321 aaatgtatta tcgacttaaa aatggcttga tacagatacg ggtggaaatc acgctgggaa
    28381 aatatcaaga ccaactctta cacttggaaa agaaattaga gagtggcttg tactgtgagc
    28441 tgacggataa agagttaaag gattcctatg tggaatatac tttgctctat gacaccatag
    28501 ccagtcgtat ttctattgat gaagtagaag ctaaagatgg taaacttcgc ttaatgaaaa
    28561 acgtatggtg ggaatatgat aagctccctc atatgttgat tgctggtggt acaggtggcg
    28621 gtaaaactta ctttatactg acactgattg aagccttgct tcatacagat tcaaaactgt
    28681 atattcttga cccgaaaaat gctgaccttg cggacttagg ttctgtgatg gcaaatgtct
    28741 actatagaaa agaagacttg ctttcttgca ttgaaacatt ctatgaagaa atgatgaaac
    28801 gtagtgagga aatgaagcag atgaagaact ataagactgg caaaaattat gcttacttag
    28861 gtctcccggc acacttctta atctttgatg aatacgtcgc tttcatggaa atgctgggaa
    28921 caaaagaaaa caccgcagtt atgaataagc tgaaacagat tgtcatgtta ggtcgtcaag
    28981 ctggcttctt tctaatactg gcttgtcaac gtccagacgc aaaatattta ggcgacggaa
    29041 tccgtgatca gtttaatttc agagtggctt taggtcgtat gtctgaaatg ggctatggca
    29101 tgatgtttgg cagtgacgta caaaaggatt tcttcttaaa gcgaatcaaa ggtcgtggct
    29161 atgttgatgt aggaacaagt gtcatatcag agttttatac tccccttgta ccaaaaggat
    29221 atgatttctt ggaggaaatt aaaaagttat ccaacagcag acagtccacg caggcgacgt
    29281 gcgaagcgga agtcgcaggt gtggactgat cttgctggct ggtgtggcaa tagccacgcc
    29341 agcacttaac ccctcggtat ctaacagggg ggtacaaatc gacaggaaac agtcaaaaaa
    29401 acattagaaa atcctttggt tacaagggat ttacaaaatt tcagcgtatg tcaaatgggc
    29461 tttaaaagtt gacatacgcc tttttgattg gagggatttt tactgaatga acaaacttgg
    29521 ttacagcatt taaaagaaaa acgcttggct tatggactat ctcaaaaccg tttagctgtt
    29581 gcgactggta ttacaaggca gtatctaagc gatattgaaa caggaaaagt caagccatca
    29641 gaggatttac agcagtccct ttgggaagct ctggaacgct tcaatcccga cgctcccctt
    29701 gaaatgctgt ttgattatgt aagaattcgc tttccgacaa cagacgtaca gcaggtggtc
    29761 gaaaacatct tacaactgaa actgtcctat tttcttcatg aggactatgg tttctattct
    29821 tattcagagc attatgcttt aggcgacata ttcgtccttt gctcccatga actggacaaa
    29881 ggagttctgg tggaattgaa aggtcgtggg tgcagacaat ttgaaagcta tcttctggca
    29941 caacaaagaa gctggtatga gttctttatg gacgttttgg tggctggcgg tgtgatgaaa
    30001 cgccttgacc ttgccattaa cgataagaca gggattttaa atatccctgt actcactgaa
    30061 aagtgccaac aggaagaatg tatctccgtc ttccgcagtt ttaaaagcta tcgcagtggc
    30121 gaactggtac gcaaagagga aaaggaatgt atgggaaaca ccctctatat cggttcatta
    30181 caaagtgaag tttatttctg tatctatgaa aaggactacg agcagtacaa gaaaaatgat
    30241 attcccattg aagacgcaga agtaaaaaac cgttttgaga ttcgattgaa aaatgagcgt
    30301 gcctattatg cagtccgtga tttactcgtc tatgacaatc cagagcatac cgcctttaaa
    30361 attatcaatc ggtatatccg ttttgtagat aaagacgatt ccaaacctcg ttctgattgg
    30421 aaactgaatg aagaatgggc ttggtttatt gggaacaatc gtgaacgatt aaaactaacc
    30481 acaaaaccag agccttactc cttccaaagg acgctgaact ggctatctca tcaagttgcc
    30541 ccgaccttaa aggttgcgat taaacttgat gaaatcaacc agacgcaggt tgtaaaagac
    30601 attctcgacc atgcgaaact gacagaccga cacaagcaga ttttgaagca acagtcagta
    30661 aaagaacagg acgtgataac aacaaaaaaa taactcaaat acaaattcat tgaatataga
    30721 gaggagaaca tttttatgaa ttttggacaa aacct
//
LOCUS       DACEKH010000024        30721 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEKH010000024 DACEKH010000000
VERSION     DACEKH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.024"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(256..810)
                     /locus_tag="IW662_001034"
     CDS             complement(256..810)
                     /locus_tag="IW662_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ0865874.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(936..2864)
                     /locus_tag="IW662_001035"
     CDS             complement(936..2864)
                     /locus_tag="IW662_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0865875.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(3170..3886)
                     /locus_tag="IW662_001036"
     CDS             complement(3170..3886)
                     /locus_tag="IW662_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0865876.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3887..5710)
                     /gene="glpO"
                     /locus_tag="IW662_001037"
     CDS             complement(3887..5710)
                     /gene="glpO"
                     /locus_tag="IW662_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ0865877.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5724..7220)
                     /gene="glpK"
                     /locus_tag="IW662_001038"
     CDS             complement(5724..7220)
                     /gene="glpK"
                     /locus_tag="IW662_001038"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ0865878.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7356..8816)
                     /locus_tag="IW662_001039"
     CDS             complement(7356..8816)
                     /locus_tag="IW662_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0865879.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSSFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8806..10101)
                     /locus_tag="IW662_001040"
     CDS             complement(8806..10101)
                     /locus_tag="IW662_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0865880.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10433..11110)
                     /locus_tag="IW662_001041"
     CDS             complement(10433..11110)
                     /locus_tag="IW662_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0865881.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11140..12231)
                     /locus_tag="IW662_001042"
     CDS             complement(11140..12231)
                     /locus_tag="IW662_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ0865882.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12332..12766)
                     /locus_tag="IW662_001043"
     CDS             complement(12332..12766)
                     /locus_tag="IW662_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0865883.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13350..14807)
                     /gene="ascB"
                     /locus_tag="IW662_001044"
     CDS             complement(13350..14807)
                     /gene="ascB"
                     /locus_tag="IW662_001044"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0865884.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14804..16699)
                     /locus_tag="IW662_001045"
     CDS             complement(14804..16699)
                     /locus_tag="IW662_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0865885.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16757..18532)
                     /locus_tag="IW662_001046"
     CDS             complement(16757..18532)
                     /locus_tag="IW662_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0865886.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18582..20078)
                     /locus_tag="IW662_001047"
     CDS             complement(18582..20078)
                     /locus_tag="IW662_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0865887.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20272..21894)
                     /locus_tag="IW662_001048"
     CDS             complement(20272..21894)
                     /locus_tag="IW662_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0865888.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22222..23535)
                     /locus_tag="IW662_001049"
     CDS             complement(22222..23535)
                     /locus_tag="IW662_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0865889.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23780..24004)
                     /locus_tag="IW662_001050"
     CDS             complement(23780..24004)
                     /locus_tag="IW662_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865890.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24024..24392)
                     /locus_tag="IW662_001051"
     CDS             complement(24024..24392)
                     /locus_tag="IW662_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0865891.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24588..25298)
                     /locus_tag="IW662_001052"
     CDS             complement(24588..25298)
                     /locus_tag="IW662_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865892.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25316..25813)
                     /locus_tag="IW662_001053"
     CDS             complement(25316..25813)
                     /locus_tag="IW662_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0865893.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25850..26302)
                     /locus_tag="IW662_001054"
     CDS             complement(25850..26302)
                     /locus_tag="IW662_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0865894.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26437..27042)
                     /locus_tag="IW662_001055"
     CDS             complement(26437..27042)
                     /locus_tag="IW662_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0865895.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27492..28112)
                     /locus_tag="IW662_001056"
     CDS             complement(27492..28112)
                     /locus_tag="IW662_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0865896.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28114..28983)
                     /locus_tag="IW662_001057"
     CDS             complement(28114..28983)
                     /locus_tag="IW662_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ0865897.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29006..29413)
                     /locus_tag="IW662_001058"
     CDS             complement(29006..29413)
                     /locus_tag="IW662_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0865898.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29432..30721)
                     /locus_tag="IW662_001059"
     CDS             complement(29432..30721)
                     /locus_tag="IW662_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0865899.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8712 a         6675 c         5069 g        10265 t
ORIGIN      
        1 tttgacctaa gtattagaga aaaaaacggg aaaatagctc ctcatatttt gccgtttttt
       61 gagctaatac cgaaggtcag ctgattgaac accgtttacc aagaggttgt gagcctacca
      121 tttaactccg aatcacaaga aataatttca aaaacaactt ctcctgtttt tggtttggtt
      181 ggcttattat cgagaagtag ctactgggag tctatttatc aaaaaaagga atgcgacata
      241 ggtcacaccc cttttttatt ttctttgaaa catctttcgg tactcgacag catacaagac
      301 aaaagattgt aaaagaccgt aagtgatgac ggcaaaaatg actctcaccc acagcgttgg
      361 taattctggg aaaagcagca aaaggatcat gccaacatag atgacagcag tcgctacttt
      421 gccataccac ttcgctccac ttaatttgat atttttagat agcccgatta atccagctac
      481 aaacataaag ccttctttta tcaagaatag accaaacaac agccaaagcc acggtcgata
      541 ccatgccata gacaaaatca gaaaaagttg tgtcaattta tctgcaaacg gatcaagcac
      601 cttccctaag tctgttacct gattatatct ccgagcaatg accccgtcta gataatcaga
      661 caaacctgag acaacaatga ccgctcctgc tatataaaat gactgtctca gcacaaaata
      721 aagataaatc ggcagcaaaa aaatacggaa tatcgataaa agattcggaa tcgtttgtat
      781 ttctttacgc cagtctgtgc ttaatttcat tttatcgccc tacctcctca gcactctttt
      841 ctttttattt tagcttacac ggggaataaa accaatgatt tcactgtttt tatttttctt
      901 tacagccact gatattatga gctaatttga gataatcact tgctgttttc atcgtttact
      961 cttctttctt cctgtcgttt gcgcatttct tttactagta ctttatgagc tttttttgat
     1021 cctcgctcta ggtcacgctg ccgtttacgt gttttataat cgataaacag actgtctaaa
     1081 tcgtaacctg tcgggtacag atcttctgct gaacgcacca gacgaacacg ctgacgaggc
     1141 acgtggatta tttttttgtc taaatataca tcaatcgtat cttcacctat atcttcataa
     1201 actaacgctt ctttttggta ttcattgaca aatatccgat caccttttgc aaagagctgg
     1261 cgttcttttt tattctcctt cctatttgta actgctgtct taggaagaaa ttcttttttc
     1321 tgtgtccgat aggctttttc cgccaagtaa cgctctgctt gatggatcaa tttcatcgac
     1381 atatccattt tatgagcaat ccacaaagct tggctttctc ctgtttctcc aacccgcaac
     1441 tgatatttag gacgaagtgc ttcccgatcg aaagccatag cagccggcac aaaatcttca
     1501 tgatctccgg cgaatttctt gatttctcca taatgggtcg tggcaatgat caatgcccct
     1561 tgctcataca tagattccat gattgcgatc gctagtgcag ccccttcatt tggctctgtt
     1621 ccactgccaa tctcatctaa aagtaccaac gtattccttt ttatcttttt caaaatagca
     1681 gcgatgtttt gcatatgacc agaaaatgta ctcaacgcat tttctaaatt ttggtggtca
     1741 ccgacatcga cgaaaatctc atcaaagaca gcaagctctg tcccttcttt tgccggaact
     1801 tgcataccga acatcgccat caaagtcaat aaaccgactg tcttcaacac gactgttttg
     1861 cctccggcat ttgctccggt gataatcaag cctcgatagt ctttaccgat ttcaacatcc
     1921 aatggtaccg catgctcagg taaaaatgga tgtctgcctt gcttgataat gatatgttcc
     1981 gatttattga ttctaggtgt gcttccattg atttctcggc taaattttgc ccgagcaaag
     2041 atgatatcta acaccgtgat cgtttcgatc aataaatcga tcacttcttc gttttctgcg
     2101 atagcaccag tcaatgccgc caggacttga tattcttcac tgatttcttc agcttttaac
     2161 aactgatagt gttcattcaa tttagaaatc acagttggtt caataaaaac cgtcgttctt
     2221 ttatttgatt catcgataat cgttccatct attttatttt tatagctggc tttgatagga
     2281 atcgtatagt attcgccttt ttgaacaatc attgcttctt ggatcatttc tttgtttttc
     2341 ggatgacgca agaatttcag taattttgac tggatttctt tttcgattag ttgtaattgt
     2401 ttgcgtactt tacgcaaatt acgtgacgcg tcgtcactga ctttattatt ttttattttc
     2461 tggtcaatca actcttctac atttatcaaa tcaggaagat gtttgctata agcaaacaac
     2521 aagggtgtct ggtattgatt cttatcaaaa aactttgtga tcatccgact gcttctaaga
     2581 aaatccgcat attcgatcaa atcggttggc tgcagaatca accctttttt gacttgttct
     2641 gtcagtgcat cgatcctcgg caaccccata aatggaacat gctgactgct ttctaagatc
     2701 aagcgtgctt ctcttgtttc ttcttgacga tccaagacag tttgtaggtt tgtcgagacc
     2761 gtcatatcgc tgattcgttt tttgctatag tttccaattg ctcttgcttg gatttccttt
     2821 ttgatccgat caaattgtaa ttgactaatc atttcgttgt ccatattctc tccttttctt
     2881 gaagagatac ctgtcccaaa ataaataagg gcctagagat aaataaaagg atagaacaca
     2941 gcttaaaaaa gtcatgtcct atccttatta gaatacaaag atattgtttt cctgacaggt
     3001 accgtttcat aagcacacca aataaacttt gtttattggc tattcgactt actgattaga
     3061 taccagctac actcacaaaa cataccccat tcttctaggt cttatttatt ttccacttaa
     3121 ctatagcaag acacattttg gttgtcaaac tttagtatgt tttatagatt tacaccatta
     3181 atgaatagac acctaccgcg ataattgctc cgatcattgg acctgcgatt ggtacccatg
     3241 aatatgccca atctgaagtt cctttattag ctattggcaa taccgcatgt gctaatcgag
     3301 gaccaaagtc ccgagcaggg ttgattgcat atcctgttgg ccctcctaat gaaagaccga
     3361 tggctaagat caatccacct acagcgaata cgttcgttcc aggtgcaaat tcgttttgtc
     3421 caaaagctag caacccaaga actaatacaa atgtaccaat taattctgtg atcacatttg
     3481 ctggataatt ccgtatagca ggacctgttg caaacgttcc taaaatcgcg ctttcatctt
     3541 tcgttgcatt ccaatgtggc aaataagaaa gccaaacaag aatggcccct gcaaaagctc
     3601 ctaaaacttg cgcaataatg aaaggaacga caagattcca actaattgcc cctgtcatcg
     3661 ccatagctag agagactgca ggattcaaat gagctgggcc catgaatcca gaaatataga
     3721 cagccattgt tacagcgagt ccccaaccaa atgcgatgac aatccagcca gaagcctgtg
     3781 ctttactttt gtttaaattc acagccgcac acacaccatc tccaagcaaa actaggatca
     3841 gtgtcccaat aaactccccc atgatttgag tcatatcact gttcatttat ttctccccct
     3901 ttagttcccg cagatctgat tctgcaatca atgcttctaa acgtttagtc tgttgcgcct
     3961 tctcctcttc tgtccattca aaatatccag caattgcatc gatcacaggt tgcttgatct
     4021 catcaagctg atctcgttca aacaacagat gatttgtacg acgaatgaga taatcgcctg
     4081 gggccaaaac catttcttct tctaaaccat aacgtaaccg agctgactca gcgagactca
     4141 aaccaggata aggtgccatt tcttttgcta attcaaagat acgtcgggca ttagtcccgt
     4201 aaaaatcagc gatataagta gcatcttctt cctctaaacc ggctgctact ccttcttttg
     4261 ctaattctgt cactgtttct tctaatttag ttggatcgaa gtttccacct gaaatctgat
     4321 attttttaga gtcgatttct ttcgtctcta ttccgtattc ttcttttaat aattggcgta
     4381 tcaatcgcaa agctccttct gccatcttac ggtaatcggt gattttccca ccagataaag
     4441 tcagcaaacc atctggttct ctttctaaag aactacctct ggaaatcgta gaaggtgctt
     4501 tttcatcacg gctgttttcc aaatggttca acacgtcttc tacttcagca cgagaaactt
     4561 tgttttcctt atattcactt actgtatcga caactttatt gaagctcttg tctgaaatcg
     4621 atccattatc tccaccattg taatcagaac cagaattacc aatcaacagt ggacgaagtc
     4681 ctgcccagct tgcttcgata tcattcaatg tgatatttgc ttctggatag cgatgattga
     4741 tcacatccaa tagataatct acatcttctt gtgtgacttt cggatccgta aaatctcctt
     4801 ggtaatccgt atctgtcgta ccaaagtaag tcttgttttc tcttggaata gcaaaaacca
     4861 tccgcccatc ttgctttcct gtatcgaagt atgtgggttg cggtacaggc agttttttcg
     4921 catctacaac taaatggatc cctttagttg gacgcatttt aggagagact ggacgcgtaa
     4981 aattcaagtt tcgtacttta tctacccaag gaccactcgt attgatcact aattttgctt
     5041 tgatttcgat cacttcatct gtcagcaggt cacgagcttt gacgccaacg atttgatcgc
     5101 catcatataa aaagccaacc gctttcattt tacttactag ataagcccca tcttcggcag
     5161 cctttttgat attatcaatc actaaacggg catcattgtt gcggaaatct agatagacac
     5221 ccgcgccttt tagcccttct tttttcaaaa acggttctct ttccaatact tcttctggtg
     5281 tcagcgtata attctcatat ttggttcctg tcacatttgc cagtttgtca taaaggtcca
     5341 ttgctacttt gaccgagaac atattgaagg ttgttgctcc ttcgccctca tagattggca
     5401 aaagcattgg atctggtttt gggatgtgtg gggcaatact ttgtacgact gcacgttcac
     5461 caactgtgtc agctactact tccacatcaa atgtcttcag ataacgaata ccgccatgaa
     5521 caagtttggt cgagcgagag gaagtccctt ctgcaaaatc ctgcatttcg atcaatcctg
     5581 ttttgattcc tgatgctgct gactggactg ccacaccggc accagtgatt ccgccaccga
     5641 tgatcaacag atccaattct tcttgctgca ttttttgaat gctatcttgt cttgtcttgt
     5701 ttgaaaacat atggatttcc tccttattca aatgcttggg tagcttttac tgcttttttc
     5761 caaccgttgt aaagtttttc tcggcgctct tcctccattt gaacttcaaa tagcttgcca
     5821 gcttcataaa attcgcggat ctcgtctgta tctttccaat aaccgacagc taatcctgct
     5881 aaaaatgctg cccctagcgc cgttgtttcc aaattttctg ctcgttggat cggtacattc
     5941 aagatatctg tttggaattg catcagatat tcattgtttg ctgcaccgcc atctactttc
     6001 aatacaggaa tcttgattcc agtgtcatct tgcatcgtat cgatgatatc tctcacttga
     6061 taagcaatcg actgtaaagt agctttaatg atatcttctt ttgtcgtacc acgtgtcaag
     6121 ccaaacatag cccctcgtgc tgcctgatcc caataaggag cacctagccc aacgaatgca
     6181 ggaacaacat aaacctcatc ttcgcttgta gagcgtttcg ctgcgtcttc tgaatcgcta
     6241 gctttttgca acatttgaag tccatctctc agccattgaa tcgaagaacc agccacaaaa
     6301 atacttcctt ctaaagcata gtaaaccttt ccatttatgc cgtaaccaat tgtcgtcaat
     6361 aaattatttt tggataattg cggtttttca ccggtattca tgacaataaa tgaacctgta
     6421 ccataagtat tcttgaccat gcctggttcg aatgccattt gtccgaataa tgcggcttgc
     6481 tggtctcctg ccatccctga aattggtgct tcgcttccat aaaaatgata accttgcgta
     6541 taaccataaa cttctgagtt agaagtgact tttggtaaca tcactcgagg aatatttagt
     6601 aaatctaaaa tctcctgatc ccaatctagc tgatggatat tgaacaacat cgtccgactg
     6661 gcattcgagt aatcagtaac atgtgcttgt ccatcggtca gtttccatac taaccaagaa
     6721 tcgatcgttc caaacatcaa ttcgcctttt tcagctcgtt cttgcgcgcc ttctacatga
     6781 tcaagaatcc agcgtatctt cgttgctgag aaataggcat cgatcactaa ccctgttttt
     6841 tcgtggaaaa attcttgata gccttcattt tttaagttat ctgcaattcc agctgactgt
     6901 ctggattgcc aaacaactgc atgatagatt ggacgtcctg tatctttttc ccatattact
     6961 gttgtttcac gctggttcgt tatgccaatc ccagcaatct gatttggttt gatccctgat
     7021 tcgatgaacg ctccggcaat aactgactgt acagaattcc agatttcatt tgcatcatgt
     7081 tccacccaac tttcttgcgg gaaatattgc gtgaactctt tttgagaact gccgatgtgt
     7141 cttcctttct tgtcaaagat aattgctcgt gagctagttg taccttggtc aatcgccatg
     7201 atatacgttg attctgtcat tgtgattccc tccattatat gcaaacgctt tctttaacta
     7261 ctcttatcat actactgttt taaaaaaaga aactgtcaaa attttattct ttttttcttt
     7321 ttttgacagt tttttattat gaagctattt tttaatcaat tcttttcttt tttcagcgtc
     7381 tctattcttc ttttcaatcg aataatgtcg taaggtgacg caaattctga cagataatat
     7441 tcacactgtt ttttgtttct ctggcaaaat gtgatcacca aatcatattc tttaccttct
     7501 tcataacttc caatctcaat accgccgatt cccctcagtt ctctgatcag atattgttga
     7561 aaagcaatac gataaatcgg cagactttct aaatcgattc caatcactac tggttttgca
     7621 atataaaaat cgatcatcaa aagaacattg gcatactgga tcatcaggta actaagctca
     7681 ctatcttcag ctcttttcct cggcaattgc tgcaccgctg tttcctgtaa tttcgccagt
     7741 aactgtgtta aggaatgccc gagcattttc tgctgctgtt ccaaaagccg atcacgctca
     7801 tagacttcga tctttccaac aaagaaaaac cattcattgt tgatctggga aatttggtag
     7861 cctactgcct tttcttcttt gattttcaaa cgacgaggac gataatgcca taacatcgtt
     7921 tcccgaatgt aagtatctaa aacagcggtt ggtaactttt tgctccgtgt caaatcatac
     7981 tgataaaata tttcttcgtc tactatcgaa aaagaaacaa aagagctgta aaacatcatt
     8041 gtttcgtata cattcagttc tgctgctgtt cgactaagat gcatcacgat gattggatcg
     8101 attgcttgat aaagccggtc cgactgatag agtaatttct tttctttcaa agttgcgtac
     8161 gtactttttt cattcaatag tctttttttc gtaatcccga gccagcaagc aatctgtgct
     8221 tctgctgatg ccgtaaacgt tgtttctaat acacggtcca atcctagaat gaacggtctt
     8281 ttttctggtg tattttgcaa aaaaagcggt cgctgatctt ccgggatata ttggaatagt
     8341 tcataataaa aatatcgtat ctgtagctct tctccgtata attgaccgtt tttgatttgt
     8401 aatccaaatt cctctaagag ctggttcaat tctttgatct ttcgaaaaac agaagactca
     8461 ctgatattaa acgctgtcgc taatcgaacg atggatactt gctgatgttc taataaatag
     8521 acgagcatct taaacttcaa gctttcctgc aataaaaaca atagaatttt ttccaaactt
     8581 acatcttcgt ccaacacaag cagcgcctga tactcatttc gctgtacttc tacttttttt
     8641 tccatcattc ggccaatcaa ttcaatatct tccaaatatt gatcaaacga ggcttttgac
     8701 agttggacat gctctctcaa accattgata gaggctgttc ccccttgcag aacaaggttt
     8761 cgcaaaatca ggatctgtaa ctgctctttt ctttctaata atgcttcaat cttcataggc
     8821 agtattcgcc acctttgacc acaccatatc tggcacacgg tattctggct ggaaaaatag
     8881 ctcatgttct tctaactgtt caaacgtcca tccttctttt actgcaattg tcagttggtc
     8941 aagaaggtgg aacatctctt ttttgctttg gagctgtgct ccaagaatct ttttttcaga
     9001 atcaaataga aatttgagtt taactttgct ttgatttatt gctgtaagct tgtagtctac
     9061 gtatcctgtt tcaatttttt ggggataaaa aatgctctct tcttctgtca aacctacgct
     9121 tcctaagaaa tagccaaacc actgattgcc gactggacga taggttccct gatcctttgt
     9181 ctgtgagcca ctaattgtct tgctgacatt tcgtgctgta cggacagcgt tatttactag
     9241 agaaacatac attttttcat ttgttggacg aaaagttact tggatcgcat cgccaatcgc
     9301 ataaacgtct ttttgagacg tttgcaagta gtcatctacc cagatcgttc catcatcatt
     9361 caatgttaat tgttcttccc acatctgatt gtcggggcga gaatgattag ccagcaaaac
     9421 aagatcatag ttctcctttc ttttttccgt ctctacaata gcggtttcat tttcggtcaa
     9481 agacacaact tgttcttcta aaaaaagtga aagcccttga tttttcattt cctttactaa
     9541 cggttctgtc atttcaggat ctagataacg aaaaagaatt gactttcggc tttcgtaaag
     9601 atggatttgt ttttttcttg atgccaaccc ttcagctatt tcgattcctg cttgtcctgc
     9661 accaataatt gcaattttct ttgcattcat gatcttttgc tgaagagccg ttacgttttg
     9721 tatcttttta actgggtgaa taagcgttga ttctgcagaa atatttcgaa acttttgatt
     9781 tgaaccagtc gctagaatca aacgatcaaa atccaatttt tttcctcctg ctaaaacgac
     9841 ttttttttct ttcaatccta ttactgtctt ttctgtgtaa acatggattc gatagcggtc
     9901 agtcagttct tctttcgtga tccaagaaag ttgtttttca ctggtaacct ttccttttaa
     9961 tatcaaattg aacccgctgg gaatccatcc taacttcgat tgcttttcaa tcaaatatat
    10021 ttcacttgtt ggcatcaatt ggcgaagata aatagccgca tgaatccctg caaacgaccc
    10081 gccgacaatt acaatgttca tctgcattcc tctttcttat aaggtgttct actgctaact
    10141 tctcaatcag aaaacacagt attcataact cgctttcaac cttatgatac atagcgatgc
    10201 agcagaatca accctctcca tcataccgtg ttctaggtct gcttctcaat aatgtactag
    10261 tggacctaat tgaagagtat catttctgaa gagatgtttc aaaaaatgta ctcttataaa
    10321 agaaaaaaac tcgcctttta cgtacctctg tccaaatagg aaacagaaaa gaacgaaaag
    10381 acgagtttta gcgtgtgtaa tgtaatagtt agaatagaca catcatttat ttttaattct
    10441 ttaatgcttc aacaactgct tcgcctaatt gatgagcaga ttgaggattt tgccctgtaa
    10501 gaagccgccc atcaatttga accacttctg aaaaagctgc tccagactga tagtttgctc
    10561 ctttttcttt caaagcatct tctgctaaga atggtacttc attcgttgta ccatttgctt
    10621 cctcttcttc attactgaac ccagtcactg tacgtccagc taaaatagat tttccagact
    10681 catctgtaac tggcaacaat cctacaacac cgtgacaaac agctgagacc agcccttcat
    10741 ttcgatagat ttgtgaagcg actttcccaa gatctattgc ttctggaaaa tcccacattg
    10801 ttccgtggcc accagcataa taaaccgctg cataatcatt tgcatccaca tcatcaggac
    10861 gtaaggtatt tcccaaggcc cgatttctga aatcttcatc ttcataatag gcccaatcga
    10921 tttcatccat attcaaaaca ctttgcggtt ctaaaggtac atatccgcct ttaggactga
    10981 caaaatctgc atcgatccca ttttttacta atacatcata aaaatgagtc agttcactta
    11041 accacaaacc tgtaggacgc tcgatctttt cgtacttttc tacatttgtt aatacgatca
    11101 atactttcat tttcccatcc tctttctata aaaattgcgt tattgataac gtgctttcaa
    11161 gaaattatat aatgggcttg gcaaataaag ctctttatat gcttcttcaa aaggtttcgt
    11221 tacaaattca tcctcacggt tttccaataa tttctctgcc cagtcaggat cgacgatcat
    11281 ttgctgtcca attgctgcaa gttctgcata tttcaatacc tgctctacat cttcgcgatt
    11341 gcggatccca ccgacaccaa ctaaagggat ctttccttga atcgtgttat ggaccacttc
    11401 aagaatcgat ttatcgttgt atttatcaga acgagctatt cgatcataca tatttaatga
    11461 aacatgcaga taatctagtt ttgactcacg taatcgttca agaagccaga tcgtgtcttc
    11521 aaaacggata cctggtgttt catattcttc tggtgagaaa cgatacccta cgatgaatgg
    11581 ttttttagca tattgttcaa ccgctgagaa aaccgttgtt aataattctt caataaaacg
    11641 ataacgattt tccaagctac cgccccattg atcctctcgt ctattcgaat gcggagagaa
    11701 aaattgttgg atcaaatacg tatttgctcc atgcaactcg attccatcaa atcctgattc
    11761 aatcgctcta cgtgtggctt tgccaaatgc ttggattgtt tcaacgattt cttcttcact
    11821 cattgctcgt ggtgtttccg catcaggacg ttttgctgca actgcacttg cagatacagg
    11881 ctgttctcca gataaagttg ctcgacttgt cattcgtcca ccgtggaaaa tctgaataat
    11941 cgcttttgcg ccttctcctt ggattgcttt cgctagctca cttaatcgag gaatcatttc
    12001 atcatgagcg atgctgagtt cacctggcca gccttttccg atatcttgga catttgcagc
    12061 accagtaata acagcaccaa gacctttaga acgttgttta taataagtga tctcatcttc
    12121 ggtgatcact ccattgaaaa agctgagttg tgtcgtcata ggcgacagca tcaatcggtt
    12181 cttcaattga actcctgaag gtaaagtaat tgattgatca aattgtgaca ttgtcattcc
    12241 tcccataatt cgctctgatt aacctatcga gaatcatcta tcattaaacc actcgattag
    12301 ttgtacatgc aactaatttg cttaaaaaat tttatttttt ttgtaactta tctgttgcat
    12361 cattcattgt ctgggctaat tctttacttg cttcatttcc caaaactgct ttgtatgact
    12421 gatgaagttc ttcccagcta ccatcgactt ccttagcaaa ttggcgtcct tgttctgtaa
    12481 gataaactaa tgacaagcga ccttcgctaa ctgttttgac gtatccttta tattctaatt
    12541 tttcaacaaa acgagcaatc gttgatgggg cggaatagag catttgtgca atatcttttt
    12601 gtggtattcc atctttttct tttaatatca ataaaataaa accataagtt ggtgccagac
    12661 ctgtccttct aaacgcttca tcagctaatt tattcaacat gcgatctagc tttttcacgc
    12721 taaagaaaag acattcatct aacgcttttt caattggctt ttccatttca tcccctccat
    12781 tggcgtacat atcatagtat gatttttatt ttttgtcaaa aaacacggaa taatgtccgc
    12841 ctcctctgtc tagatttgaa aaaaagaagt agccatcttc aaagaatttc ggcttgtttt
    12901 cgacaaaact ccttctggaa caccgtgaat cagaggttgt gaaaaagtcg tttagttttg
    12961 agtatgaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat ttggcttaat
    13021 accgaaaaac tggcttttga acaccgttta tatcagaggt tgtgaaaaag ccgtcctgca
    13081 tcaagtaata agaacagcca aacaaaaata gttcctcata ttttgtcgtt ttttgagcta
    13141 atgccgatgg tcagctgatt gtacaccgtt tattgagagg tcgtgagcct gccgttgaac
    13201 tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gttttatgac tatggtgact
    13261 tgtgatcgag gagttggcag gagaatactg tttattcgta aagaaggggc tgtgacaaga
    13321 cttttgtcac agtcccttat ttccttttat tataggattt cgccatttga ttggatgact
    13381 tgtctgtacc aatcaaatga ttttttcttt aaccgtttct ttgtgccatt cccttcgtca
    13441 tcttgatcaa catagatata tccataacgt tttgacattt gtgaagtaga agcgctgacg
    13501 atatcaatgc agccccacga cgtataacca aataaatcta ctccatcttt gatagcttcc
    13561 ttcatctgct caatatgctg cttcaaatag tcaatgcggt aatcgtcttg aacggttcca
    13621 tcttcttcta ctttgtctat agctccgagt ccgttttcag ctatgaacag tggtttttga
    13681 tacctatcat ataattgatt caaagcaatt ctcaaaccct taggatcaat ctgccagccc
    13741 cattcacttg atggaagata cggattttta attcctccag ttattaagtt tccttggacc
    13801 ccttcccaat tttctggatg agcactgact gcactagaca tgtagtagct aaaagaaaca
    13861 aaatcaacag ggtaggctgc taagatttct tcatcaccta cttctatttt taagttgaca
    13921 cctttttctt caaacattcg ttgcgtatac gctggataat atcctcttgc ttgaacatca
    13981 ctaaagaaca aagaagttct ttgctgctca attgtttttt gaatatcatc cggatcacaa
    14041 gaatacggaa caagaagctg atagctgatc atacaaccga cttgtgaacc tttgataatt
    14101 tcatgacaag cttttgtagc taaagcactt gctataaact gatggtgtgc accttggtat
    14161 ttcgcttgtt ccaaatgggg atggttttct tcaattactc ctacgctaac aaaagggtga
    14221 tgttttacac agttgatctc attgaaagtc atccaatatt ttactttatt tttatagcgt
    14281 ttgaatactg tttcagcgaa acgaacgtaa aactgaataa gctttcgatt aggccatcca
    14341 ccatattctg taaccaaata taacggcatt tcataatggg aaagagaaac gactggctca
    14401 atcccatatt ttagacattc gtcaaacact tgatcataaa aaaataatcc cgcttcattg
    14461 ggtgtttctt catcaccatg aggaaaaatt cttgtccatg cgatagacat ccgatagcat
    14521 ttaaaaccca tttcagcaaa taaacggatg tcttctttaa agtgatgata gaaatcattt
    14581 ccgtgtcttt taggataata atacttgctg ttgggatcct gcgccgcttt tacttgctga
    14641 tgcgtcatca tatgccattg atctgtccat ttttctttag gtaaatcgct gtcgaatgta
    14701 tacacatcag acacagataa tcctttttca tctacatccc atgcaccttc acattgattg
    14761 gccgctgtcg ctccgcccca taaaaatcct tctggaaatt ttttcatatc gatcccccta
    14821 aacttgctag ttttcttgtt tgattatttt tcttatgact agtaatgata tttttgaatc
    14881 tactactatt tgtaacaata accattgtgg tcggatcata tccctctttg attaattctt
    14941 gcaaatctac ttccatcaat aaatcgcctt tacgtacttt ttttccatca gatactgcac
    15001 tttgaaagta tttgccattt aaattcactg tatcaatacc gatatggatc agtatctctg
    15061 ctccttcttc actgactacc ccaattgcat gtttcgttgg aaaaactgtt gaaacttccc
    15121 cgtttacaga actgtagatt tttccgtccg ttggaatgat cgcaaaccct tctcctaaag
    15181 atccactagc aaatgttgga tcgtttactt cagataaatc aattatttca cctgctgcag
    15241 gagtacttaa cactatatcc tttgaggatt cttcttcgat ttcatccttg tagccgaaaa
    15301 gaaaagttag catacaggaa ctgatcatcg ctaagaaata actagcaact agccaaacaa
    15361 atccttcacc aatataaatc gggaaagaca aaatacttct tgtagcaact gccaaagcac
    15421 cggctcctgc ggcaccagct actccaccaa atatagctcc acatatacaa gcaatgtaaa
    15481 atggtcgttt atatttaaga gtcacaccat aaacagcagg ttcagtgatt ccaaataatg
    15541 ctgtgataaa tgcagataaa gataaacttt ttactttctt atttttcgtt ttcaaaaata
    15601 ccccaagtac tgctcctgct tgcgcagcta cggcagtcat acatgctgga cctagcgtat
    15661 cacgaccgta gaaagaaaga ttgttggtca ttactggtac aagtccccaa tgtaacccaa
    15721 aaatcactaa cacttgccaa aatccgccga taacagcacc agataaaaca gggctaaagt
    15781 tataaacagc taaatatcca gctgcaatca gctgacttgc ataatctgca attggaccta
    15841 caactaataa aataattggg accatcacga tgatacaaat gaatggtgtc aataaatttc
    15901 gaatactttc gtgcaagctt ttgcgaatct taggctcaat ataagaaaga acccatacag
    15961 ctaaaattgc tggaataacc gtacttgtat accttgctag aatcacgggc atccctagaa
    16021 atcgcaaggt tatgctatca ctatatgcac tgacaatcgt aggataaacg agagctgctg
    16081 caataaccat agcgatgaaa cgatctgtat tgaacttttt tgcagcagta tatgccaaaa
    16141 atataggcaa gaaatagaaa aatgcatcag ctgctgcatt caaaatgata taagttcctg
    16201 attcagtagt taataaattc aaactcgtac ataaggcgag tactcctttg atcaaaccac
    16261 ttcctgccaa gacgcctaaa gtcggggcaa aaacagcggc gatcatgtct ataaaagagt
    16321 ttaaaatgcc ttttttctca gttcctctct ttccactaga tgcatcatct tctagtttca
    16381 tttgtttgga taatacgtca tacacttcat ttactgcatt tcctactacg acttgaaaca
    16441 ttcctccagc ttcgactaca gtgatcacac cttctagttg ctctaccttc tccttatctg
    16501 ccttcttatt atcttttagg gtaaagcgta accttgttgc acaatggtaa acattgctga
    16561 cattttcttc gcctccgaca aatcggacaa tatctttcgc taattgtgaa tagtccattt
    16621 ttacttcccc cttgttaatt aatgccgtag caatccttct tacaagataa gtatagttct
    16681 tagaaaacgg tttctccatt caattagtac cccttctaat agcgaacgaa actgtacaat
    16741 agtgtgtatg gtttacttag ccgagcaaat cactcactgc ttgaaagaaa acaagtatat
    16801 cgtttctctt atagtcaaag gagacaaaaa aaacacgctt gatcttcctt tgctgatgta
    16861 taagtggtat ctcaagctga tactctgtga tttttgtttc tacttggctt ttgataagac
    16921 agcacaacaa agtctcggca tttaacggaa gcataactag gtcagaacta ggagagatta
    16981 gttgctgaat ggaatctatc tttttatgga tttttatttg attttcttct ttgtagtaat
    17041 gatcaagcag ttttgttttc tttttttctt caactatggt cagcttttca cgcaaaatct
    17101 gataaatctc ccaagattca aactcatcta gaacactagg tagataaatc aaatcatccc
    17161 actcaaaaat aagctcttgc tcagtagtca aaattgcatc atattccgtt attttgtttg
    17221 attgattttt gaaaagatac actggttcta tccggcattc tttgatccac atgttcatac
    17281 tttgttgaat ctgttttttt agtgtattca aaatactaat tggctggtta ctaacaatca
    17341 gtatctcccc ttgttttcga aaaggattca acgcttcggc aagatacaaa acaaaataag
    17401 cgctttcagc taaattggga cgtaattgaa aatgtattgg gaagaaacgc cgaattaaat
    17461 aaatttctaa aggataacgg gagatgatct tttgtacaaa atgattcatg gtattatgtc
    17521 cggcatccat cctcgttttc atttgtttga tatgggttag caaaaattct gtttcatgga
    17581 acaataacga aacaggaaga tctaaaaaac gaataatcgt ttgctcaaat gctcttagat
    17641 tcttctctat ttctttgttt ttttcatcat taatcatcaa ataatttaac tctaatagtc
    17701 gattttcaat tgcttgtttt tcttgattgg aaaaagaata gggtagttct gatgataact
    17761 tttttgtgag ggtctctacc atcggaataa ataatgtata cctgtttgtt ttttctaaaa
    17821 gttcttctct tcgaagaatg gtcaaaacga gataccgaga aaatcgtatg aagtcctctc
    17881 ctgaaacaat ataattatat gatataagaa cctcttttaa tgtcttttgg acagtcggca
    17941 tgatattgga aaaaattgta ctgatttgtt gaggaaacct tgcgtgttgg atcaccgttt
    18001 ctgttccaac aagagataag aaaattcttt ttgaattttc ttcagcaatg atttttagtc
    18061 cttttgtcga cgaaacttcc agttctaatt taggattgcg ctcgatccaa cgcaaaatcg
    18121 tttttatttg taatgatacg gctgtttttg aaagaaagaa aatgtctgct atcttttgca
    18181 tactgacata ccctttttga aacaataagt acgtcacaat agttgaagct cggaaatcca
    18241 tgctggaata gattttatgt ttcgtcacct cttgtatgat ttgctgttta gtttcagatt
    18301 caattgattc taaaaaataa cctgaacgtt tatttgactg gataatgatt ttcttctcgt
    18361 agatagcaag catatttttg atttcagttt ggatcgtttt cttgttggta cctaaagctt
    18421 cagctaattc ttcgcttgtt atccaatgat tcttatttct taatactaca agaatttgga
    18481 gttgtcgttt atttaagtcc atttgcttac acctcctctt tgttataaac atacaggaaa
    18541 aacctctcaa atgacaatag tcacttgcag aggtttaaaa atcatttgaa gaaagatcta
    18601 tattcaaggt ttttaccatt agactggata atttgtttat aatattcaaa actatctttt
    18661 ttcaatctcc taaagcttcc gctcccatca ttttgtctat ccacataaat aaagccatat
    18721 cgcttcctca tttcccctgt tcctgccgat acgatatcga ttggtcccca ccataagtaa
    18781 ccaatgattt ggcaaccatc ttttattgcc tcagcaattt cctgaagatg tatttttaga
    18841 tactcacaac gttttggatc gtggattcga ttcaattcat ctggtgcttc atctagtcca
    18901 atgccatttt caactatgaa taatggtaat tcataacggt ctgtcaattc attcatgaca
    18961 taacgaaacc ctagaggatc aattggccaa ctccaaggtt cgggagattt ttccttcaaa
    19021 aagggattat cgaaaccatt tgctccgcca gtatctacgc gcatttctgc ttctttttta
    19081 tagacagcgc tacgataata actaaatgca atataatctg ccgtgttagc ttgcaagcac
    19141 tgttcgtctc catctaacat tactggtgca cagtcttctt ccttccatgt cctgtaaata
    19201 taagctggat aatgccctcg gcacatgaca tcggagaaaa accatgaatt gcgtttaaat
    19261 tgcagtgaac caaatacatt ctctggatca cagtcatatg cataagtcgc taatgcagaa
    19321 aagctacaca taatacccat cttagcattt ggatcgattt tctttaataa ttgagtagca
    19381 cgggcgttcg ctagaaacat atgatgacag gcttgataaa tcactttttt tgttagtgta
    19441 gcaatgggtt caagtttatc tctagcaggg aatgtaacca atccagcagc tgcataaggc
    19501 atacgaaaag cattattgat ctcattaaag gtcatccaat atttcacttt cccttggtag
    19561 cgttcaaaga cggtctgcgc ataaaattca aaaaagtcga ttaattttcg attttgccac
    19621 cccccatact ctcctaccaa tgctaagggg gtttcataat gactaagagt aattactggc
    19681 tcgatatcat atcgatttaa tgtattgatt agtctgtcat aaaatacgag accagcctca
    19741 tttggcttct gttcgtctcc tcttggaaat attcgtgccc atgagatgct tgttcgaaaa
    19801 gcttttaaac ctaactcctt cagtaaacga atatcctctt caaatcgatg ataaaaatca
    19861 atcgcttcat gagaaagata atggagtgat tcgtctaaag caatctccca aatctttttt
    19921 tcttcattcc actgaatact aggatgctgg ttcatgatcc cgataagcgt atctgtcaca
    19981 ttaggtaatt tgccgtcttc caaccatgca ccttcacatt gattagcagc tactgctccg
    20041 ccccataaaa aattttctgg aaatatttcc tgatccataa ctatctctcc tcgctatcaa
    20101 caatttctta cttgaagtat agctcgattc atcaaaacaa gatattctaa ctatttcggc
    20161 agaagagaag aaaaaaattg aatgctactc ttcattttta atatttatat ataacaaata
    20221 cataagacga tccccgaaag aaaacactcg ttaaccatct aagcaaacga tttattttac
    20281 ttccaacaca aatgcttcat aaggtcgtaa atcaactttg ccgtctggta actgtttcag
    20341 aggataattt gacacaataa cccttaaagc ttcacgtgga atagtgaacg tctgcttttc
    20401 actggacata ttacaaataa cgagccattg ctggtcttca tatatgcgag aataggcaaa
    20461 gacatgctca ggcgtctttt ccactaattc atagtctccc cagacgatga tgtcatgttt
    20521 tctacgcaaa tcgatcaatt tcttgtacat ataaaaaaca gaatcttgat ctgctaacgc
    20581 ttcttctgca ttgatttctt tataatttgg attgactcga agccatggaa ttccagtagt
    20641 aaacccagca tgttcacggg cgttccattg cataggcgtc ctagcattgt ctcttccttt
    20701 cgccttgatt gattccaaga tttcctcttt tgtaaatcca gaagaaatcc tttcgttata
    20761 catattgatt gtctcaatat cttttgcttc cgcgatatca tcgattgggc aattcgtcat
    20821 gcctaactct tctccttgat agatataagg ggtgcctttc atcatatgga gcaagatagc
    20881 aaacatcttg gcactttctt ttcgatacac atcgtcattt ccccaacgag aaacaattcg
    20941 cggaagatcg tgattattcc aaaataaact attccatcct tcatcaccta aagatgtttg
    21001 ccatttggat aatacttttt tcagttcaga gatactcaag ggtttcaaat cccatttgtc
    21061 tttgccttct tgctgatcta atcctatgtg ttcgaattgg aagaccatgg ataattcatt
    21121 tcttttagga ttggaataaa gtttagcgat ttcggtagtt gctccccatg tttcgccaac
    21181 agtcatcaga tttttgtcac caaaagttgc ttgattcatt tcctgaagat actcatgcag
    21241 tttaggtcca ttacttgtga tttctttatc cggttctttg ccaatcatat cgatcacgtc
    21301 catacgaaag cccccaatac ctttatcgat ccagaaattc atcatttgat aaatctcttg
    21361 gcgaacctgt ttatttttcc aattcaaatc aggttgacgc ttgctataca agtgcagaaa
    21421 atattgcccc gttttttcat ccaactgcca agcagaacca ctgaatattg agcgcaatct
    21481 attaggctct tttccattga ccggatcacg ccaaatataa tagtcccgat aaggattttc
    21541 tttattttta cgggcctcaa tgaaccacgg atgttcatca gaagtatgat tcaccaccag
    21601 atccatgatg atacggatat ttcttttctt tgcttcttca atcagttctt ccatttcttc
    21661 catcgtaccg aattctgcca tgattgcttg ataattactg atatcatacc cattgtcatc
    21721 attcggagac tcgtatactg gagaaagcca aattgcatca atacctaaat acgccagata
    21781 atccaacctg gaaataatcc cccgcaaatc tccaatacca tcttgatttg aatcttgaaa
    21841 acttcttgga tagatttgat aaacaactga atggtgccac catttttgtt ccatgatttt
    21901 cctcctataa atggatgttt ctattttttg cttaatcatt tattttagtc tctctcacta
    21961 aaaattgtaa cgctttcaaa tagctattgc accttgttat cggtaacaga ataaaacaaa
    22021 aaaacagctg aggaaattcc ctcaactgtt ttttgaaagg attgatttat gcagggaaga
    22081 atgctgattt cacaacttct ttataaacgg tgttcactca gctgtccctt ggtattagct
    22141 caaaaaacgg caaaatataa gaaactattt tttcgttttt ttctctaata ctcaggccag
    22201 aacgctgatt tcacaacctc tttatttcac attcaatatc cacatgaatc ccacaaatac
    22261 aaagaacaga ccatacatga ttggagatac ttcttttcct cttttagctg ctaacatggt
    22321 aattggatag aagataaaac ctaatgcaat cccatctgaa atactataag tcaaaggcat
    22381 gccgatcaag atcaagaatg aagggatcgc aatctctaag tttccccatt cgatttgtcg
    22441 caaagattgt gccatcaata ctcctaccat aatcaatgct ggtgcagtta cttgtgaagt
    22501 aacaactgat agtaatggag aaaagaacaa gcctaacaag aagaaaatcc ctgtagtaat
    22561 agctgtgata cctgaacgtc ctccaacggc gataccagct gatgattcta caaatgcacc
    22621 aacaggagaa gtaccaaata aggaacccgc aagcatagct gttgaatcag aagctaaagc
    22681 tttccctaca cgaggcatct tgttgtcttt catgaaacct gcttggtttg ctaatccaac
    22741 taaagttcct gctgtatcaa agaaagtcac aagcaagaat gtcagcacta cgacccacat
    22801 ttgaagagaa ttgatgtccc ctacatgttt taatgcaact aaaaatgttg gtttcaaact
    22861 tggtgctgcg gcaacaattt cagaaggcat tggaatcaaa cctgtgacca gacctaaaat
    22921 cgttgtagct gccatcccga tgaagatgcc acctggtaca cgccgaacaa gcaagatagc
    22981 agttacgact aatccaaaaa tcgtcaacca agtagaacca acgttgagtg atcctaatcc
    23041 tactaatgta gattcattcg aaacgatgat cccaccttcg ctcagaccaa ggaaagcgat
    23101 aaatagaccg ataccgccag aaatcgcata tttcaaatct gctgggatcg catcaatgat
    23161 catttcccgc agcttgaaga ttgtgatcaa aataaaaatc aaagaagcaa cgaatacgcc
    23221 ggctaatgct gtttcccatg gaatccccat tcctaaacaa acagaataag caaaaaaagc
    23281 attgatccca agagcaggtg ccgttccaat tggataacgg gctaataccc ccattaaaac
    23341 acagccgatc gcactggcta atgcagtcgc agtaaacact gctccttcat ccattccaga
    23401 agcacctaat acagttggat ttacaaataa gatataagcc attgagataa atgttgtaaa
    23461 tcctgccagt acctcacgtt ttaccgtggt gttcaatttt tctatttcga agtaggaaag
    23521 tattttttcc ttcatcttcc atccccctaa atccgaacat tataaataaa gtccgttaaa
    23581 atttcgattt attaataaaa caacgtgttt tatcatatac taaaacaaaa cataagtaaa
    23641 caaaaaattc ggattttaag caaaatccga acgttccatc tgattaaatc cctttatctc
    23701 aaagttcatg aacattcact ttggattaca agaatttctt tattgtgtta ataaagtttc
    23761 ttcattctag tctcaaaaat caatagtgtg atttgatttt accatttcgt ttctgttctt
    23821 ctttgatgaa ttcttttgta tcgacaggat tcagattatt tagtccgcca aaacctgtat
    23881 cgaccatttt tccccagcct tttgtcttct tttttcctga tttgagattc tttgctcgaa
    23941 gcttctcact atgttttgct cgccgatact tcaaataacc ttctaatctt cccattagat
    24001 ccactgtttt tcctcctttt tattcaatca gacgactcag cttttcctat aacaaaataa
    24061 ttgccttcag ggtcagaaaa attgaaagtc aagatgccat tgttatctgt gatttctcct
    24121 ggttcttcca tgttctcgta gatagtttca aattcatccg taaaaaatac taaagaaggc
    24181 ggtggtccta atacctctgg tgaaaacttc tgcacaaata cttttgggaa tacgcctaat
    24241 tctaattctt ttgagatgct gagtacaata ctcttaaatt cttctggcaa ttcgattgtt
    24301 tcagaaattt ctgcaccaat tttttctaca aagaatcggc aaatcatctc tacgtcattg
    24361 acataaagca tcacacgtgt tttttgtatc atcatttgac ccttctttct atttgatttt
    24421 ctttcctgtc tataaactga ttccattata tcacgaaagc gcaaacacct tgtgtttgat
    24481 actattcaag aaaaataaag caatagtttc taggattgct ttcctccaca aacttttttt
    24541 acaaaaaaaa aacggcaatg aatgccgctc ttcttatttc gattctttta taattctgct
    24601 tctgattcat ctttggttag tgtgtgtcta acactagtta cgccaaatgt ttgatcgatt
    24661 ttttccttca acgcttcgat tgcctgtgcg ccacgtgccg cttgttcagc attgatggat
    24721 tcaatgacca gcaccgctgc ttgtgtttct tccgtcagca tcgttctttg ggcttcacgc
    24781 cagttgagac agcgacagat cgctccttgt tcatcatagt agatgatttc ttccggaagt
    24841 gccggagcat cggaatctgc acctaaaggc aggaatgatt cgccaccttg ggcttttcct
    24901 aaatgcagat ttcctgcgat cttttcgata tcttctccgc cgcaaggtac gccaaaagac
    24961 aaggaaatac tattatagat atctacaagc ggattgattg ggaaaaattc ccgttcttga
    25021 tttgcacgtt tcagtaaagc ttcaatagaa gaacgtgcac cttttttagt cttgaatgtg
    25081 gtaaatgcct gacgccactg agcaatgact tcgttttggc tgaatgtttc ttctgtaagg
    25141 taatttcttg cttcttttgc tgcctcattc aataattgat gatacgtttc ttcactttct
    25201 tttgttacgt ggttatcgat accatttacg gataagattt cgatggttgc ttcaggaaat
    25261 acttcccaga aattggtatc cactattact tttttcatct gttcacacct cttgattaga
    25321 ataattgctt gatgaataga tagtctgttt ggggatcatc tcccatataa aaaacatgtt
    25381 cgctgtattt gataaatcct tttttacgat aaaaagcgag cgcaacatcg ttgtgttccc
    25441 aaacaccgag ccaaatggct gttttttgct tttcttttgc tttttcaata gctgtatcta
    25501 gtaaaaatgt accgatacct tgatgtttaa atttttttcg tacatagatt cgctcgattt
    25561 ctaaaaaatg atcagccatt tcctcagatt gactcgttcc tgtatttagt ttcaggtaac
    25621 ccgcaggctc ttgatcgata ctcactagga aaaaatcggt ttctttatct tttagctctt
    25681 ctgtaagttt ttctagtgca taagcttgtg tcaagtaatc agctaaatgc tccggtgaat
    25741 tatcttttcc aaatgtatct gtaaatgttt cgatactaat ttgttgtagt aagtctacat
    25801 ctgaaggcgt catttttcga atttcgtatt tattcacggc ttcttccttt caatagatcc
    25861 ttttattgcc ttttttgaca aaattccaat cttcgctaac attcttgcat actttattta
    25921 aaagatattc caattgtttt gcttcttcgt cagaaagacc atttaatgcg actgcattgg
    25981 agtagttgtt ttctcgaatg atgaaaggat agatttctgc tcctttttta gtcggataga
    26041 gtttcttgat ttttttattt tctttatctt ccaaacgttc gatcatccca ttggattcta
    26101 atttcttgat tgctcgggca gtcgttgtac gatctacttt gatcatttct gctagttttt
    26161 cttggatgat tccaggattt tcgcaaatac gtactaaata aagatactgt cctcttgtta
    26221 aatcgacttc tttgaattcg atattgctaa tagaatctag cgctcgggca atcattccga
    26281 tgtctcgaag gatttcagcc atttcatccc acctttattt tgtttgtaag actagttcac
    26341 ctctttttgt tgcaaatgca actattttca ataaaaaaat caagttcttt tttaataact
    26401 agtaaagtat attcttttct tcttttacga aaaagctcaa tcacaataga tctggttttt
    26461 gttttctaaa ataagtaact gtttcttcgt tgttcgcttt tctgatagct ttggcaactc
    26521 ttctatacta aaaaatccca tgttggaagt ttcattattc tcaataaact tggcctcttc
    26581 accgtggatc gcacaagcaa aaacaagttt ataatactga aaaagctgtg gaatatcttt
    26641 ttgtttattc gtatcaaaaa ctgctcgcaa ctgagtagtt tctactgtta atcctgtttc
    26701 ttcatagact tctttacgca cattttcttc aggagacaat cctatttcag caaatccacc
    26761 aggaagtgac cattctttcg tccgtttatc ttcgattagt aggaccttgc catctttttt
    26821 gatataggca cgaacatcta cttttggtgt tggatgtcct tctccaatcg ttgtgagcgc
    26881 ttttatttct tcgaaagttt cttttcctat ttcactgatc agttccaatg aaatcgtccg
    26941 gatctcctca aaacgctctt tatcaaatac atcttttcca taatatagac cagcttgaga
    27001 aagtgcgagt agtcttttgt attgagccaa cttcttgttc attctcttcc cttctttttt
    27061 agcaaatgta tgactatgta atcaagtata gaagactggt ttaattacgg caaataaata
    27121 taatgaaatc tgcaataaat aaaagaatgt taaaaaactc gagaacaaaa gacattcgac
    27181 ttctctgggg ctgtgaataa atggcgttca aaagcttgca tctgcggtaa taagccccac
    27241 caaacgaaaa atatgacgag ctattcttgt ctggctgttc ttattaattg aggcaggaca
    27301 gctttttcac aacctcccca ctaaacggtg ttctcctacc aactcctcgg tcacaagtca
    27361 caatattcat caaacatgag aagctgtttt tttgaaattg ctccttgtga ctcggagctg
    27421 aacgacaggc tcacgacctc tcggtaaaca gcgttcaaaa gccaattttt cgatattaag
    27481 ccatttcttt gttacttatt gtcattgatt tctttttcta atttatccaa tagtggagtg
    27541 taatccactt gagtatagtc tgttacatca aattcatata gtgttcctaa ctggaatttg
    27601 tcatattttc ggtcggcgat gatctttttg aatgcttctt tcgtaatatg attggtccct
    27661 ttcgttcgat cttccaatac gtcaccatag tgataatgat ccatgatata gctcaaatga
    27721 cgatctgatt cacgatcacg tttgtaccga cgttgccata acacttcaaa gtctgctgtt
    27781 aggcgaatcg tgatcacctg atatccgtat ctatctgcta attcttggag tcgtgctttt
    27841 tgcttatcac taaaaggata ctcggacaca atcactttct ttcctgcttc catatacaaa
    27901 tcaagtgctt tgtaatagaa aggataaact ttcgtccttt cttgttccgc ccgttcttcc
    27961 aaattgttaa acccgatgga ttctgcatag tattccttga tttcatcgag cgtgatggca
    28021 tacatatctg gaaatcgttc ccgtagttta ttcataagat atgtttttcc tgtaccagga
    28081 ctaccagcca gaagaatgaa atactttttc atctcattta tttcctaagt taattggact
    28141 ttccaagttc ataaacagtc caatcttatg cttgacaatt tcttttactg cttcatttgc
    28201 tggaatcaca ttatggcgaa gagacttgtt gatttctgtt tcactaaagg tttctttagc
    28261 tactttcgtc cagttgacta aaagctccgt tccaacattg aatttgcgga tcccgttatt
    28321 gatcaattca ggataatctt cttctttcac acctgttcct ccatggataa ctagggggac
    28381 atcaactact gcatcgattt ctttcagcaa gggaatgttt acttctgttt ttgatttgaa
    28441 ttgtccatga ttcgtaccga tagcaatagc taatgcatcc acgcctgtag cttctacaaa
    28501 tttcactgcg tcttccggtc ttgtatatac cttgtcattt tcagcgacat ggattccttc
    28561 ttctgtaccg ccgattgttc caagttctcc ttctacggaa acaccgtgcg catgggcata
    28621 ttctactact gcttttgtct tcaggatatt ttctttgaaa ggcagatggg acccatcata
    28681 catgacagaa gtatatccgc tgtcgattgc ttctttgata tcatcaaagt ctcgggcgtg
    28741 gtctaagtgc aagactgcat caaccatttc ttcttctgca atagctgtca ttacattgac
    28801 taacacatgc atgccgatat atttagcggt atcgacactt gtttgaataa tgatcggcga
    28861 acccatttct tttgctgcgc ctagcatagc aggaagcatt tctaggttat gcgtattaaa
    28921 agctccaact gtaaaattca actcttctgc tgtttttgtt acttctttca atgttgtata
    28981 catcttttat tgccacctta tctttttatt tgtttccttt gatcgttact tgttttgcaa
    29041 tttgtcgcat ctcatccatt ttgcccatgt agtaatcgat cccttgatca tcaccagcac
    29101 gagcagcatc tgcacgcttc tggttataga ggctcatcag ttttttatac ccatctccga
    29161 tcgtctgttt attttccaaa ctcttttcta aatattggat ggcttgatcg ttcacataca
    29221 attcagaaaa tttaagtcca acttgttcat aaagtttcgc taatttatgc ttatcttggg
    29281 tagtggaaat ctcttgtttc aattgtgtaa tctctgattg aatcttttcg gtcgcttctg
    29341 gcgatagggc tttttcttct tttgccagaa cttttttcgg ttctttttct tttttcttaa
    29401 aaaatccaaa catcattttc cctactttca attaaaatgc tttcaaaact gggcttaaga
    29461 tccaggcata taagagtgct gcggctactg tatctacatc ggttgctgta gcattgttga
    29521 atcccatcga attaaagatc gttactagca aagcgggcaa taatgtaata aagaaaccat
    29581 gagcaatacc gccaatgatc gctcctcggc gtccgccaac tgcgttgcca aatatccctg
    29641 ctgtccctcc ggcaaagaaa ttggttagca taccaggtaa aatcatcgct aagccgaaca
    29701 tgggcaaagt aaacatggca ataatcgtac caatcgttgt cgtaatgaaa cctaaaatga
    29761 cagcatttgg gctataagga aagaaaactg gacaatctaa ggcagggatc gcatcaggaa
    29821 ctaatttcat agctatccct cgaaatgccg gcacgatttc acctaacagt agacgtacac
    29881 cagctagcaa tacatacaca cccactacga attggatggc ttgtaaaaag gcaaacatga
    29941 tatagttttg atctcctgat aattcagaag cgaatggttc gcctgcaaat aaaacagtga
    30001 tgatatatag cgggaccatc acgaccatga cagacaaata agtatcttgc agaaattcga
    30061 aatgtgtcgg caacttgata tcttcgatcg actttttatt ttcgcctttc tcaccaaaaa
    30121 gcttagcaac accggcttca aataagtacc cgatcgtaca aaaatgacct aaagcaatat
    30181 catttgatcc agtgatttta cgaatgatcg gttgcgctac tgccggcata gcaatggcga
    30241 aacaagctcc gataaaacca ccgaccaaga tcaatacgat ccctcgcaga ccggcaaaat
    30301 agccaaagac tgtcgtcatc gttgccatcc ataaaattgc ttgaccagtt aaaaaaatgt
    30361 acttccattt tgtgaaacga gccaaaataa tattgaatac aaaaatggct aagaacgtca
    30421 gtgcaatatc tctacctaaa cccaaatcat tcattgcttg accattgata gattcaatag
    30481 aagggatgat cccttgcata tgaaatcctt cagtaaagat tttcccgaaa taagttaaac
    30541 ttccaacgat gatgctcgaa ccggcactca acacttgaaa tcctaggagt gtctttaacg
    30601 tgccagagat gacttgtcct gctgatttct tctgcagtag tagacccaac atagcaatca
    30661 atgcaatggt gatcgatgcc tgagtcaaaa tattttcgat gataaaattg ataacgccca
    30721 t
//
LOCUS       DACEKH010000025        29631 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEKH010000025 DACEKH010000000
VERSION     DACEKH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.025"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(133..306)
                     /locus_tag="IW662_001060"
     CDS             complement(133..306)
                     /locus_tag="IW662_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0865900.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="IW662_001061"
                     /pseudo
     CDS             754..3116
                     /locus_tag="IW662_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="IW662_001062"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="IW662_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ0865901.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="IW662_001063"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="IW662_001063"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ0865902.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="IW662_001064"
     CDS             5704..6489
                     /locus_tag="IW662_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0865903.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="IW662_001065"
     CDS             6502..7353
                     /locus_tag="IW662_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ0865904.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="IW662_001066"
     CDS             complement(7477..7929)
                     /locus_tag="IW662_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0865905.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="IW662_001067"
     CDS             complement(8394..8762)
                     /locus_tag="IW662_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ0865906.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="IW662_001068"
     CDS             8912..9394
                     /locus_tag="IW662_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ0865907.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="IW662_001069"
     CDS             9431..10441
                     /locus_tag="IW662_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ0865908.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="IW662_001070"
     CDS             complement(10556..11269)
                     /locus_tag="IW662_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865909.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11459..12049)
                     /locus_tag="IW662_001071"
     CDS             complement(11459..12049)
                     /locus_tag="IW662_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ0865910.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12533..12760
                     /locus_tag="IW662_001072"
     CDS             12533..12760
                     /locus_tag="IW662_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ0865911.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12776..13942
                     /locus_tag="IW662_001073"
     CDS             12776..13942
                     /locus_tag="IW662_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ0865912.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            14176..15219
                     /gene="hrcA"
                     /locus_tag="IW662_001074"
     CDS             14176..15219
                     /gene="hrcA"
                     /locus_tag="IW662_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ0865913.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15313..15876
                     /gene="grpE"
                     /locus_tag="IW662_001075"
     CDS             15313..15876
                     /gene="grpE"
                     /locus_tag="IW662_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ0865914.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15927..17756
                     /gene="dnaK"
                     /locus_tag="IW662_001076"
     CDS             15927..17756
                     /gene="dnaK"
                     /locus_tag="IW662_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ0865915.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17907..19073
                     /gene="dnaJ"
                     /locus_tag="IW662_001077"
     CDS             17907..19073
                     /gene="dnaJ"
                     /locus_tag="IW662_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0865916.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19155..19820)
                     /locus_tag="IW662_001078"
     CDS             complement(19155..19820)
                     /locus_tag="IW662_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ0865917.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            19796..19969
                     /locus_tag="IW662_001079"
     CDS             19796..19969
                     /locus_tag="IW662_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0865918.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20014..20751)
                     /locus_tag="IW662_001080"
     CDS             complement(20014..20751)
                     /locus_tag="IW662_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865919.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(20753..22255)
                     /locus_tag="IW662_001081"
     CDS             complement(20753..22255)
                     /locus_tag="IW662_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0865920.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            22514..24508
                     /gene="uvrB"
                     /locus_tag="IW662_001082"
     CDS             22514..24508
                     /gene="uvrB"
                     /locus_tag="IW662_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ0865921.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            24518..27334
                     /gene="uvrA"
                     /locus_tag="IW662_001083"
     CDS             24518..27334
                     /gene="uvrA"
                     /locus_tag="IW662_001083"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ0865922.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            27597..28862
                     /locus_tag="IW662_001084"
     CDS             27597..28862
                     /locus_tag="IW662_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ0865923.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            28866..29597
                     /locus_tag="IW662_001085"
     CDS             28866..29597
                     /locus_tag="IW662_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ0865924.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
BASE COUNT         9855 a         5075 c         6273 g         8428 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
    11461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
    11521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
    11581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
    11641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
    11701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
    11761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
    11821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
    11881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
    11941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
    12001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
    12061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
    12121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
    12181 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
    12241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
    12301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
    12361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
    12421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
    12481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
    12541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
    12601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
    12661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
    12721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
    12781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
    12841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
    12901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
    12961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
    13021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
    13081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
    13141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
    13201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
    13261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
    13321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
    13381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
    13441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
    13501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
    13561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
    13621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
    13681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
    13741 aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac
    13801 aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa
    13861 tgaagaagac cgtgtttatt tgacaccaag agggaccttt ttggggaaca atgtgtttga
    13921 gcgctttttg tttgaaaaat agtttaaaga atatttgttt tttatctgaa tttaaataaa
    13981 aaaagttcaa aaatacaata aaggactatt gaaacgttga tataactgga tttacatcaa
    14041 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
    14101 ggagaagggc ttcttgctat atttataagt ggattagcac ttagaatata tgagtgctaa
    14161 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
    14221 cactatacga gtagtggcgt gcccgttggt tccaaaacat tgatgtctga gggagtcaag
    14281 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
    14341 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
    14401 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
    14461 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
    14521 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
    14581 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
    14641 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
    14701 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
    14761 cttcgtacgg agattccgat gattcttcat aaatatttcc aaacgacaga agggatgtct
    14821 cagttatttg acaccgttct tggtcatgtt tttgaagata aggtttatgt cagtggacaa
    14881 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
    14941 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
    15001 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
    15061 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
    15121 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
    15181 gctgattact accgttcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
    15241 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
    15301 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
    15361 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
    15421 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
    15481 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
    15541 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
    15601 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
    15661 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
    15721 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
    15781 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
    15841 gtgttaagac caaccatggt cattgttgca caataattgt gcggatataa aacttacact
    15901 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
    15961 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
    16021 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
    16081 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
    16141 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
    16201 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
    16261 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
    16321 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
    16381 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
    16441 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
    16501 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
    16561 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
    16621 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
    16681 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
    16741 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
    16801 gtcaagcgct aaaagatgca ggtttatcac aatcagaaat cgacgaagtt attttagtcg
    16861 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
    16921 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
    16981 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
    17041 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
    17101 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
    17161 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
    17221 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
    17281 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
    17341 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtaaaa gatgcagaag
    17401 caaatgcaga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagatg
    17461 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
    17521 aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca
    17581 tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
    17641 aactatacga acaagcggca caacaacaag ctcaagaaaa cccagaagca gctcaaggcg
    17701 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
    17761 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
    17821 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
    17881 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
    17941 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
    18001 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
    18061 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg
    18121 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
    18181 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
    18241 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
    18301 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
    18361 aaatctgtca tacttgtggt gggaacggtg caaaaccagg cacacaacca actacttgtc
    18421 ataaatgtca tggtgcaggt acgattaatg tagaacgcca aacaccactt ggacgagtga
    18481 tgagccgtca aacttgtgat gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc
    18541 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
    18601 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
    18661 gcggaccatt tggtgatttg tatgtggtct tccgtgtaga agaaagtgat atctttgatc
    18721 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
    18781 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
    18841 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
    18901 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
    18961 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
    19021 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
    19081 agtaaaaacg ctaagtcatt aataattaaa aaccagacga acaatcatat gttcgcctgg
    19141 tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat atgataatcg
    19201 tcatagtttc taggggtata accagtgaga gaatggaact tgttcaatcg atattgcaag
    19261 gtgtttttat gaataaataa ttcttctgag caatttttga tgcttccgtt atttttactg
    19321 aagcatgcca aagtcttctt gaacaaatgt acttcttctt ctgtcaggct tttcaaaata
    19381 cgttttacat ataatgttcg ttgatctgac gggattgctg gtaaaacgat cccgaggtct
    19441 aaatcttcaa aaaagcggat ctcgttcaca gattgatgga aagctatcca ttcgcaaact
    19501 tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg tataacaaag cgacccaatt
    19561 ccaaagttta gctgtaactg atacatttta ttaaactttg tctggatttt atcaagcaat
    19621 gatagcaatg aggctctgga aatccgattg actaggataa tgatttgttg tctatgtaca
    19681 gtgagcaaag cctgtccgta ataagagaag accctttcga gcaactccaa taaaggattg
    19741 atccgttctt gacacaattg ctgattgtca gaaagttgtc ccacaactac ataatatgga
    19801 gaagaaaaat caagctcaat taagtgaggg tggtatcgac tatgtacttg atatcatcaa
    19861 tgaaccgatg gtactttctt atgcgatgga acaggtagag cgattgcttg tttgtgcagg
    19921 aaaacaagcg atgcggctga tacgattagg tgagaaaata agaagataaa aatacacggt
    19981 cccttcatgg cgaccgtgca tttttggttt ttattaaata tttaatactt tattcaagaa
    20041 atcttttgtc cgttcatttt gcggattttc aaagatttgt tgaggcgttc catcttctaa
    20101 aaagtttccg tcgtcaatga acatgacgcg attggccact tctttagcga accccatttc
    20161 gtgcgtaacg ataaccatcg tcataccttg ttctgctaac tttttcatga cgttcaagac
    20221 atcaccgacc atttcaggat cgagtgcaga agtaggctca tcaaaaagca tgatgtcagg
    20281 gttcatcgct agagcacgag caatagccac acgctgtttt tgtccaccag aaagcatttc
    20341 aggatacata tcttttttat cttctagacc aactgtctgc agtaatttga ttgctttttt
    20401 ctctgcttct tctctagatt ctttcttcag gtccatcggt gctaaaatga tgttttccaa
    20461 aatagaaaga tgagggaata ggttaaagtg ttggaatacc attccgatat gttgtctcac
    20521 caagttgata tctgtgcttt tatccatcaa gtgggcgccg tcaataataa tttcaccgtc
    20581 agaaggttct tctaaacgat tcaagcaacg caagaatgta ctttttcctg aacctgaagg
    20641 gccaatgata cagacgacat ctccttcgtt gatggataca ttgatgtcgt ttaatactgt
    20701 gttatcgccg tattttttga ctaaatgctc aactaagatt ttttctgcca tcttacttca
    20761 ccttcttttc taaaactttt gctaattttg tcaatgctgt gatcaaaatc aagtacatga
    20821 tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg tgcaacaatg atcttaccag
    20881 tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt tgtgtctttt aatgagataa
    20941 cgaactgatt gacgaatgat gggatcatga tgcgtatcgc ttgcggcaaa atgatttttt
    21001 gcattgtccg attataagaa agacctaaac ttctggaagc ttccatttgt ccgacaggaa
    21061 cggcgttgat cccgcctcga acaatctctg cgatataagc acttgcgtta agagtcaagg
    21121 tgataacacc agcaaggaag tcaggaatcg taaatccaac gataccaggc aacccaaaga
    21181 aaataaagaa cgcaagaacc atcattggaa ttcctcggat gatatcgaca taaatactag
    21241 caattgtgcg aagagcgcgt attggtgcta cactgaataa tccaaaaatc acaccgatta
    21301 ctatagccaa agcaaaggag atcaaagtta ggagaattgt tctccaaagt cctttcaaca
    21361 atgcttgata gttgttttgg ataagtccaa gaaatgttga ttcatcagca gtggaagact
    21421 cactggaagt ttctgaacca tccgctacat attctttaat aatcttatcg tattcgcctg
    21481 ttctttttaa ttctttcaat ccttcattga acatattaag aagctcagga ttttgtcctt
    21541 ttttaacggc aaatgcatat tttccgccag attctttttt gatcggtgtt tccaattctt
    21601 gtccttgttt gatcgcataa ccgattaccg gataatcatc catcattgca tcgattgcgc
    21661 caacttttaa cccgtcgtac aattgatcag cagcatcaaa atatttgata gaatagccat
    21721 attcagattc atggtcgctt aaaaattcag cactttcggt accaactttt gctccgactg
    21781 tttttccttt caaatcttca taagacttga tcgaagtatc tccttttttg acagctaatt
    21841 ggatcccgct atcaaagtaa gaatcagaaa aatcatagct tttctttcgt tcatctgtta
    21901 cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc caaagcttgc aaagcagcag
    21961 agaaaccacg gtagtccatt cggatgttaa atccttgcag tttggcgatg ttttccatca
    22021 attttacatc gatgccttcg tactcgccct ttgcgttttg aaattcaaat ggagcaaaag
    22081 cagagtcact ggcaattacg tatacgtctt ttttgggctc tattttttgc attgtctcgc
    22141 ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc aacatgacca aacgcaagtg
    22201 tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa atgctttctt ttcataatca
    22261 acctcctaaa tttccaatat tttattatta taggcgaaaa gtgctgaatc agacaagact
    22321 gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat tttatgttat aaaacataac
    22381 atgtataata agataaaaaa gtggcgaaag tatgttcggt gtggtataat tttttagtaa
    22441 ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa atgaaaagga atgaatgaaa
    22501 aggaggacga agaatgattg agagagatac ctcccgacac tttgagcttg tctcgaaata
    22561 ccaaccggct ggtgatcagc ctgaagcgat taaccaatta gtggatggcg tagtcggcgg
    22621 gaaaaaagca caaatcttat taggtgcgac tggaacaggg aaaacatata cgatttccaa
    22681 tcttattgag aaagtgaata aaccaacact gattattgcc cataacaaaa cattggctgg
    22741 tcagctgtac ggggaattca aggaattttt ccctaacaat gctgttgaat attttgtttc
    22801 ttattatgat tattatcagc ctgaagctta tgtgccttca agcgatacgt atatcgaaaa
    22861 agattcaagt gtaaatgatg agatcgacaa attacgacat tcggctacta gttcattatt
    22921 agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt atttttggat taggctcgcc
    22981 ttttgagtac caaaaacaag tagtttcgat tcgacaggga gcagaactag atcgcaatca
    23041 gttgattcgt gacttagtca gtatccaatt cgaaagaaat gatatagatt ttcagcgcgg
    23101 tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg gcttcaagag atgaacgtgc
    23161 cttacgtgtg gagttttttg gagatgaggt cgaacgtatc cgagaagtca atgcactgac
    23221 aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc ccggcgactc actttgtgac
    23281 gaatgatgaa catatggagc atgccgtagc gaatatcaaa gaggaattag aacagcgttt
    23341 aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa cgtttagaac agcgaaccaa
    23401 ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg ggaattgaaa attattctcg
    23461 gcatatggac ggacgtaaag aaggtgaacc gccttatacg ctattggatt tctttcctga
    23521 agattttttg atcgtggcgg acgaatcgca cgtaaccatg cctcaaatca gaggaatgta
    23581 taatggtgac cgagcgagaa aacagatgct agtagattac ggtttccgtt tgccatctgc
    23641 tttagacaat cggccacttc ggttagaaga gtttgaaaaa catgtcaatc agatcattta
    23701 tgtctctgcg acgccaggac catatgaaca tgaacaaaca gataccgtta tccaacaaat
    23761 catccgtccg acagggctgc ttgacccagt gatcgaagtt cgtccaatca tgggacaaat
    23821 cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa gaccagcggg tgtttgtaac
    23881 gacattgacg aaaaaaatgg cagaagacct cacagattat ttcaaagaat tagggatcaa
    23941 agtcaaatat ttgcatagtg atatcaaaac gctggaacga acagaaatca ttcgagatct
    24001 tcgattaggt gaatttgatg tgttagttgg aatcaatttg cttcgtgaag gattagacgt
    24061 acctgaagtt tcccttgttg ctatcttgga tgcggataaa gaaggctttt tacgtagtga
    24121 acgttcctta gtccaaacga ttggacgagc tgctcggaat gaagaaggta aagtaatcat
    24181 gtacgcagac aaagtaacag attctatgcg tctagcgatg gatgaaacgt caagacgtcg
    24241 tacgattcag cagaagtaca atgaagaaca tgggatcgtt ccaaaaacga tcattaaaga
    24301 aatccgagat ttgatttcta ttacgaaaga atcggaagat gatacaaaag aagcggtaca
    24361 agtctcgtac gaagaaatga cgaaggaaga aaaagacacg ttattgatga aattagaaaa
    24421 agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca gccgcgaatg tgcgagatat
    24481 gattttagaa ttaaaagctt caaagtagga ggacaacatg gctaacgata aaattacgat
    24541 tcatggtgca cgtgcccata acttgaaaaa tatagatgta acgattccac gagataaatt
    24601 cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc ctagcatttg atacgctgta
    24661 cgctgaaggg cagcggagat acgtagagag cctttctgct tatgctcgtc agtttttagg
    24721 acaaatggat aagccggacg tagatagcat cgacggattg agtccggcga tttctattga
    24781 tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt actgtgacgg aaatcaatga
    24841 ctatttacgc ttgttgtttg cacgtgtagg acatccgatt tgtcccaacg atcatatcga
    24901 gattaccagt caatcagtag atcaaatggc agatcaagta atggctttgc ctgagcgcac
    24961 aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa ggacaacata aaaaaatatt
    25021 cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc gatggtgaga tctatgattt
    25081 aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat atcgcgatcg tcatcgaccg
    25141 gatcattgtg aaagaaggga ttcgttctcg cctatttgat tctttagagg cggccctgcg
    25201 attagcagac ggctatgctc tggttgacgt gatcggtgaa gaagaaatgt tgtttagcga
    25261 acactatgcc tgtccttatt gcggatttac tgttggcgaa ctagaaccaa gactgttctc
    25321 attcaatgcg ccttttggag cttgtccaga ctgtgatggt ttaggtgtca aattagaagt
    25381 cgatcaagat ttagtgattc cagatagaac gaagacactt agtcaaggtg ctatcgttcc
    25441 atggaatcca atcagctctc agtattatcc tcaaatgtta gcgcaggctt gtgaaagttt
    25501 cggtatcgat ttagatacac cattcgaaga attaccggaa gagcatcagg agatcatctt
    25561 gaatgggaca gatgatcttt tccatttcca ttatgaaaat gattttggtg gcgttcgcga
    25621 tgtagaaacg actttcgaag gggtattgaa gaatatcgag cgtcgttatc atgatacgaa
    25681 cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca gaattaactt gtcagacatg
    25741 taaaggttat cgattgaatc cccaagctct atctgttcaa atcaatggaa cgaatatcgg
    25801 gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc ttctcaaact taacgttatc
    25861 tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa gtaaatgaca gactttcatt
    25921 cttggaaaat gtgggactag actatctaac tttgagtcgc gcttccggaa cattatctgg
    25981 gggagaagcg cagcgaatca gattggctac acaaattgga tcgaatcttt ctggagtttt
    26041 gtatatttta gatgaaccat cgatcggatt gcatcaacgc gacaacgatc gactgattgg
    26101 ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt gtagtcgaac atgatgaaga
    26161 tacgatgtgg gcagcagatt atttgattga cgtcggacct ggggccggag aacaaggcgg
    26221 acaaatcgtg gctgcaggca ctcctgaaga agtagctaat gatgaaaatt ctttgacagg
    26281 gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa gaacgccgta aaggtaatgg
    26341 gaaagcaatc aagatcactg gtgcttcaga aaacaatctg aaaaatatta gtgttgaatt
    26401 tccattagga gaatttgtag cagtcacagg tgtgtctggt tctggaaaga gtacattggt
    26461 caatagtatc ttaaagaaat cgttagcgca aaaattaaat aagaattctg ctaagccagg
    26521 taaattcaag acaatttccg gctacgaaag tatcgaaaag atcatcgata tcgatcaaag
    26581 cccaatcggc cggacgccga gaagtaatcc agcgacttat acaagtgtat ttgatgatat
    26641 ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg ggttataaga aagggcgttt
    26701 tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc ggggatggaa ttattaagat
    26761 cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa gtatgtcatg gtaaacgata
    26821 taattctgaa acattagaag tgcattacaa aggaaaaagc attgctgata ttttggaaat
    26881 gacagtagag gatgctgtag aattcttcaa gcacattcca aagattcatc gcaaactgca
    26941 aacgattgtt gatgttggct taggttatgt gacgatgggg caaccagcaa cgacattgtc
    27001 cggtggtgag gcacaacgga tgaaacttgc cagtgaattg cacaaaatct ctaatggaaa
    27061 gaatttctat atactagatg aaccaacgac aggacttcat agcgatgaca tcgcccgctt
    27121 gttgcatgta ttacaaagat tagtagatgc tggtaacaca gttttagtga ttgaacacaa
    27181 tctagatgta atcaaaacag cagattatat cattgattta ggaccagaag gtggagaagg
    27241 tggaggaacg atccttgcga ctggaacacc agaagaaatc attaacgtaa aagaaagtta
    27301 tacaggtcac tatttgaaaa aaataatggt ataaagagga tagatagctg agatagttaa
    27361 aaaaacataa aaaaatgaat ttttgcttgt tttattaata tcagtgttct agaatcatta
    27421 catggtagtt ctttctacac tgattggtat agataaaaac ttcctataag ttcaatccgt
    27481 tcaaaaaaga attgagcaat cggaggactt tcctttgatt gcttttttct ttatggtatg
    27541 atattccttg tgtagttgga aaagaaaaac aagagattat gagtaagtga gagataatga
    27601 tgaacagtat tgaaaatgaa gaatttatcc ctatattatt aggaagcgac atgaacgttt
    27661 atggtatggc acgttctttt aatgaagcat acggcaaaat ctgccaagca tatgcttcgg
    27721 atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt agaagttatt ccaggatttg
    27781 ataaagaccc tgtatttatc gagacgatgc ttcgtttggc aaaagaaaga tattctgata
    27841 agagtaaaaa atatcttttg attgcttgtg gtgatggata tgcagaattg atctcccaac
    27901 ataaacaaga gctgtcagaa tattttatct gtccatatat tgattattct ttatttgaaa
    27961 ggctgatcaa taaagtaagc ttttatgaag tttgtgaaga atatgatctt ccgtatccta
    28021 aaacattgat cgtcagagaa gaaatgttag taaatggaca tttggaacag gaattgcctt
    28081 ttgaattccc agttgctttg aaacctgcaa acagtgtgga gtatctttct gtccaatttg
    28141 aaggaagaaa aaaagctttt attttagaga ctagagaaga gttcgattta attttaggcc
    28201 ggatatacga agccggttat aaaagtgaaa tgattgtaca agattttatc ccgggtgatg
    28261 atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga tcatcaagtt cggatgatgt
    28321 gccttggtca tccactatta gaagatccta caccagcatc gattggtaat tacgttgtga
    28381 tcatgccaga ttataacgaa aaaatctatc aaaccattaa ggcatttttg gaaaaaatcg
    28441 aatatacagg ctttgccaat ttcgatatga agtatgatcc tagagatgga gaatataaat
    28501 tatttgaaat caatttgaga caaggtcgta gcagtttctt tgtgacattg aacggcttga
    28561 atctggctcg ttttgtcaca gaagatcgag tgttcaacaa accctttgtt gaaacgacct
    28621 atggaacgaa tcaatcagat aaagcaagat tatggatggg tgtgcccaaa aaaatctttt
    28681 tagaatatgc tcgggaaaat gaagacaaaa aactagctga gcaaatgatc aaagaaaatc
    28741 gttacggtac tactgtattt tatgaaaaag accgatcaat caagcgctgg ttgttgatga
    28801 aatatatgtt ccataactat attccaagat tcaaaaagta ttttcatgtg aaagaaggct
    28861 agttcatgga gaattttttc agtattttag gcggaatggg cacgatggcg acagaaagtt
    28921 ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga tcaagaatat ttgaattatg
    28981 tcttattcaa tcatgcaaca gttcctgatc gtacagctta tattttagat cgatcagaag
    29041 aaaatccaat gccgtttctg ctggatgata ttgagaaaca aaatttattg cgaccaaact
    29101 tcattgtgtt gacttgtaac accgcacact acttttttga agagttacaa gcggcaacgg
    29161 atattcctat cttgcatatg ccaagagaag cagcaaatga acttgtccgt cagcatacga
    29221 cagggagagt agcgattcta ggaacagaag gaagtatgaa agctggaatc tatgaacgcg
    29281 aagtcaaaaa tcttggtttc gaaacagtga ttcctgatac tgcgcttcaa gaaaaaatca
    29341 attacctgat ctatcatgag atcaaggaat cggattattt gaatcaagag ctctattacg
    29401 aaatcttaga ggaagcggta gagcgcctaa actgtgaaaa agtcatccta ggttgcacag
    29461 aattatcttt gatgcatgaa tttgctgaag ataatcatta cccagtgatc gatgctcaat
    29521 ctattttagc ggatcgaacg atcgaacgtg cattagctga aagaagcgag gcattggata
    29581 cagcatcgga aaagtaaaaa aattggattt ggggcaaata tcctaaaaga t
//
LOCUS       DACEKH010000026        28597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEKH010000026 DACEKH010000000
VERSION     DACEKH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.026"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            235..1875
                     /locus_tag="IW662_001086"
     CDS             235..1875
                     /locus_tag="IW662_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ0865925.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2029..3351
                     /locus_tag="IW662_001087"
     CDS             2029..3351
                     /locus_tag="IW662_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0865926.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3413..3844)
                     /locus_tag="IW662_001088"
     CDS             complement(3413..3844)
                     /locus_tag="IW662_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0865927.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3908..4744)
                     /locus_tag="IW662_001089"
     CDS             complement(3908..4744)
                     /locus_tag="IW662_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ0865928.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4934..5236)
                     /locus_tag="IW662_001090"
     CDS             complement(4934..5236)
                     /locus_tag="IW662_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ0865929.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5262..5693)
                     /gene="ruvX"
                     /locus_tag="IW662_001091"
     CDS             complement(5262..5693)
                     /gene="ruvX"
                     /locus_tag="IW662_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ0865930.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5690..5956)
                     /locus_tag="IW662_001092"
     CDS             complement(5690..5956)
                     /locus_tag="IW662_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ0865931.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6210..6935)
                     /locus_tag="IW662_001093"
     CDS             complement(6210..6935)
                     /locus_tag="IW662_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ0865932.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7301..7371)
                     /locus_tag="IW662_001094"
     tRNA            complement(7301..7371)
                     /locus_tag="IW662_001094"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7337..7339),aa:Gly,seq:ccc)
     gene            7488..8240
                     /locus_tag="IW662_001095"
     CDS             7488..8240
                     /locus_tag="IW662_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ0865933.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8256..8735
                     /locus_tag="IW662_001096"
     CDS             8256..8735
                     /locus_tag="IW662_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ0865934.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8986..10566)
                     /locus_tag="IW662_001097"
     CDS             complement(8986..10566)
                     /locus_tag="IW662_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ0865935.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10559..11302)
                     /locus_tag="IW662_001098"
     CDS             complement(10559..11302)
                     /locus_tag="IW662_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865936.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11478..12254)
                     /locus_tag="IW662_001099"
     CDS             complement(11478..12254)
                     /locus_tag="IW662_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0865937.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12502..13338)
                     /locus_tag="IW662_001100"
     CDS             complement(12502..13338)
                     /locus_tag="IW662_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ0865938.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13349..14278)
                     /locus_tag="IW662_001101"
     CDS             complement(13349..14278)
                     /locus_tag="IW662_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0865939.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14470..14823
                     /locus_tag="IW662_001102"
     CDS             14470..14823
                     /locus_tag="IW662_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0865940.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14897..15553)
                     /locus_tag="IW662_001103"
     CDS             complement(14897..15553)
                     /locus_tag="IW662_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0865941.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15779..17713
                     /locus_tag="IW662_001104"
     CDS             15779..17713
                     /locus_tag="IW662_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0865942.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17799..19022)
                     /locus_tag="IW662_001105"
     CDS             complement(17799..19022)
                     /locus_tag="IW662_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ0865943.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19016..20914)
                     /locus_tag="IW662_001106"
     CDS             complement(19016..20914)
                     /locus_tag="IW662_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ0865944.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20908..22026)
                     /locus_tag="IW662_001107"
     CDS             complement(20908..22026)
                     /locus_tag="IW662_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0865945.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22109..22942)
                     /locus_tag="IW662_001108"
     CDS             complement(22109..22942)
                     /locus_tag="IW662_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0865946.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22935..23852)
                     /locus_tag="IW662_001109"
     CDS             complement(22935..23852)
                     /locus_tag="IW662_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0865947.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24079..25203)
                     /locus_tag="IW662_001110"
     CDS             complement(24079..25203)
                     /locus_tag="IW662_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0865948.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25386..26294
                     /locus_tag="IW662_001111"
     CDS             25386..26294
                     /locus_tag="IW662_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0865949.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26435..28048
                     /locus_tag="IW662_001112"
     CDS             26435..28048
                     /locus_tag="IW662_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ0865950.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8889 a         5621 c         4868 g         9219 t
ORIGIN      
        1 ctatttatct atctaacata tcctttattc ttctactttt ctttctttag catataagag
       61 atacgcatag taatcaagca aggattgttg atataaaaag aacaaatacc gctattttga
      121 tttcttttga ctctattact catataaata aaatttatta tacctgctct ttttgactat
      181 atcctcatct gactcctata cttcgtttgt attaaaactt aggaggaatc attcatgctt
      241 acagggctaa acttattaaa taatcctttt ctaaataaag gaacagcttt tacaaaagaa
      301 gaacgggcaa aatacggaat cactggtatg cttccaagta ctgtccaaac attggaacaa
      361 caatctgtcc aagcttacgg tcaatactta agcaaacaga ccgatttgga aaaacgtatc
      421 ttcttaatga atctattcaa tacgaaccgg acattgtttt ataaattgat gggcgaacat
      481 ttagttgaat tcatgcctgt cgtttatgat cctgtcgttg cggattcaat cgaacaatac
      541 aacgaaattt tcttgaaacc acaagatgct gtatttcttt caatcgacga accagaaaat
      601 atcaaagcaa gtttgaaaaa cgcagcagat ggacgcgaca ttcgtttgat cgttgtgaca
      661 gatgcagaag gaatcttagg tatgggtgac tggggtgtga acggtgttga catcgctatc
      721 ggtaaattga tggtctacac tgccgcggct ggtatcaatc ctgcacaagt cttgcctgta
      781 tcaatcgacg ctggtactaa taacgaagaa ttattgaata atccattata cttagggaat
      841 cgtcatgctc gtgtagaagg cgaaacttac tacgaattca ttgatcaatt cgttcaatca
      901 gctactgaat tattcccaga attgttatta cattgggaag attttggacg aggaaatgct
      961 gcaacgatct tagaaaaata cgaagataaa atcactactt tcaatgatga tatccaaggc
     1021 acaggaatcg ttgtactagc tggtgtatta ggtggattga atatatcagg tgagcaattg
     1081 aaagatcaaa cgatcttgac atttggtgct ggtactgctg gtgtaggtat cgcaaacatc
     1141 cttttagatg aaatgatccg tcaaggtgta cctgaagaag aagcacgcca acatttctat
     1201 caagtagata aacagggttt attgtttgaa gatacaaaag gattgactcc tggtcaaatc
     1261 ccattcgctc gtaaacgctc agaattcact aatgctgatg aattgacaaa cttagaagca
     1321 gttgtaaaag caatccatcc aacgatcatg atcggtactt ctacccaacc tggagcattc
     1381 actgaagaaa tcgtgaaaga aatggcggct cataccccac gtccaatcat catgcctttg
     1441 tctaatccaa caaaattggc agaagcaaaa gcaaaagatt tgattgaatg gacggacgga
     1501 aaagctttag ttggaacagg tatccctgct gctgatgtgg aatataacgg tgtaacttac
     1561 caaatcggtc aagcaaataa tgcgttgatg tacccaggtt tagggcttgg tttgattgct
     1621 tcaacagcta cacgagtaaa tgctgaaatc atttctcaag ctagtcgagc attaggcgga
     1681 atcgttgatg taacaaaacc aggagcagct atcttgcctc cagtagcaaa aatcactgaa
     1741 ttctctcaaa cgattgctga aactgtcgca aaatcagttg ttgcacaaaa attgaatcgt
     1801 gaagaaatca cggatatcaa agaagctgtt gaatctgcta aatgggttcc tgaatataaa
     1861 tctttagaag attaagattg ttatagtaaa agaacataca gaaactttgt atgacgtcta
     1921 aaaagaaaaa agcgactgcg gcaaaacagc agccagccaa ctactgctct tttgccgctt
     1981 ctctttggct tttttcatac gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa
     2041 acaacaaccg caactgcagt tgaggaaaag aaaaaagaag ggttttggtc aacaaaaatt
     2101 accggcgtaa gtttgcccct ttatttgatc atggtccttg tcctgatcat tgcaatggct
     2161 ttaggaaaat taccgacgaa tatgattggc ccaatcgctg ttttggtcat tttcggtaat
     2221 ttatttcatt ttatcggaac aaaaatccct atagtcaaaa gttatttagg tggtggctcc
     2281 gtttttgcga tctttgcctc tgccgcgatt gctacatttg ggatcttacc tgagtatgta
     2341 gtaaaatcca cagaaaactt tgtcagcaat atgggctttt tagactttta tattgctgcc
     2401 ttgattactg gtagcatcct tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt
     2461 tttattcccg ttgcacttat ttcaatggtc gtctcctttt ttgccgtagg actcgtaggt
     2521 atgcttatcg gaaatggttt tgcaaattcc gttttatata tctcattgcc cgcaatggct
     2581 ggtggtgttg gagccggagc tgtaccttta tcaacgattt atgcaaatgt tttaggtacg
     2641 gacgcttctt ctatcatttc ccgcctgatt cctgcgacag caatgacaaa tgtcctagct
     2701 atcattggtg cagcgttagt tgctcgtgcg ggacaatcaa tgccaaaatt caacggtaat
     2761 ggtaaattaa tggaaaaagg cgaccttggc gatggaaaac ctagaaatgt aaaaccttcc
     2821 attccacaat taggtgtcgg tttgatggta tcattgactt tcttcatctt aggacaaatc
     2881 tgtaactatt ttgttcctaa agtccatgct tacgcattca tgatcattat cgttgttatc
     2941 tgtaaaatcg caaacctatt accagaatat tacgaagatg cagctatcat gttcaacaac
     3001 ttgattgtca aaaacttgac agcagctgtt cttgccggta tcggtattgc gttattgaat
     3061 ctaaatgtat tggcttctgc tttgacatgg caatttgttg tcttatgttt gacaagtgtt
     3121 atcgcgattt cagtagtttc aggatttgtc ggtcgtttgt ttggtttata tccaatcgaa
     3181 tcaactatta ctgctggctt atgcaacaac tcgatgggtg gtacaggaaa tgtagctgtc
     3241 ttatctgcag ctaatcggat ggaacttatt gcttttgctc aaatgggcaa tcggcttggt
     3301 ggtgccatcg ttttgatcat ctcaggtttc ttgatgcaat tactttctta gtcaaaaaaa
     3361 atacctgatc atcaagtatc ggcttgatga ccaggtattt tttgataaaa atttacagga
     3421 ttttcaaaaa tcttttttgc attttatcgg aatgataacc gatgcgttca taaaatcggt
     3481 gtgcttctac tctgtattct gcagaattaa gtcttattgc tgatattcct cgtcttttcg
     3541 cttcttctac gccagtcatc aaggcctttc cgattcctct tttttcagca tgtttagaca
     3601 cagcaagtgc cataatattg aacatcggat cgctgaaaat cgattcatag gactccgctt
     3661 gtacatagcc agctgctttt ttagaagtag gatcttcata gaccaagaaa aaattttttt
     3721 ctggctcctt caacagtttc ttcatttgtc tcaccgtaac ggttaatggt acatcatatc
     3781 ctaattgttc cgcattgatt tctcgtagtg cttctgcatc tcctaaacgc atttcccgca
     3841 acatccaaat caccagccaa tttttatttt tttaaatgaa tttttttatc acagtatact
     3901 ccttttttca cttaaaaaaa gacttttcag tgattttgac ttgcttgaat ccagatcctt
     3961 ctaatgtttg caagatgacg aaagcattgt aaagattgat caaaagaacc acgatgacaa
     4021 tcgcttgaaa aataaaggtc actttttttg gagacaaaac cctgctaaat cttgctcccc
     4081 acaaacctcc aataacagca gcggggataa cataaaacaa catagataaa tcaaagcgca
     4141 taaagctact tgttaaagca atcgttacca attttgccag ttgagaaaag aaaatcgtag
     4201 atagagaata tagtgttgct tcttttattg gcaatgcaaa catcagcatc aacaaagaaa
     4261 cattgactgg tccaccacca atacctaaaa aactagctaa aaagcccaga acccccccac
     4321 atataaggta ccaagtccat gacgtcaaat gaaattttgg ctgatgatgc ttggtgtaga
     4381 agaacgcaaa aaccaaggtc aataccgtca aaaaaatttg gatcagctgc acatgcttct
     4441 cgttttcaaa taactgtaaa aaaacttcga atacaatgtt gccgactact cctccaacaa
     4501 cagctccact agatacccaa ccgacaatct gccaattcag tgatttcccg cttgatactt
     4561 gtcgcacagt cgaaacaatg gacatcgtga ataccgcaac tgttgagtaa aaagaaatcc
     4621 ctgcaacaga atgggcgccg ataagatcaa gaatcggttt gatcaacacg cctccaccca
     4681 tcccggaaat agcgccaact gtgttcgcca gaacgatcac cacaaaataa atcagaccaa
     4741 tcatcttgta tttcctttct cttgtttata tcttttagcg attgttactc ttttctttta
     4801 ttatacagta aaagaaacaa acgaggcttc gaaagctttt ctacttttta aggaaattta
     4861 gtgctaaacg aaaaaaccgg gctctacttt tcagagagtc cagcttttct tacaccttat
     4921 cgattctttt gttctattct tcttccgcca tgaatgtatt gaatacttct tcgatcatat
     4981 cccattctgc gtctgtttcg atttgttcta actcgccttc tgttccatct tcgttttcga
     5041 tgtatgcata agcttgtaat tctacatctt catcttctga aacaccagct ggatataaaa
     5101 gaacatagtt acgtccaaat tcttcttgtc cgtcaactgt tagcaagatt tcgtataatg
     5161 tttcatttcc ttggtcatcc accaatgtga tgtgttcgtg tccttcgtgt tcgtgggaat
     5221 ggttatgttc tgtcataaga ttacctcttt tctttttatt attaaaataa ttgcttacct
     5281 gagccgtcta aataattttg taaaatcatc acagctgcta atttatcgat gacttttttt
     5341 cgtttcgctc ttgaagtatt tgcctgctct actagcatac gttctgcctg aacagtggtt
     5401 agtcgttcat cttgataatc aaccggcaag gagaatagtt ctttgatctt ctctccatat
     5461 gacatggaag cttctgccct tggtccgatg gaattgttca tattcttagg taatccaact
     5521 acaaaacggt ctacttcgta ctgcgccact aattccttca accgatcaaa accaaactct
     5581 tcttctgcct cattgattcg aatgatttct actccttgag ctgtccagcc aaagggatcg
     5641 ctcacagcaa cgcctactgt cttagaaccg acatccaagc ccattactct cataaattca
     5701 ctccatgatt tgagaggtag tctttgacaa ttacttccat gatttcatcg cgttcgtgac
     5761 ggcgaatcaa atttcgtgca tcacgataac gaggaatgta ggccgggtct ccagaaagca
     5821 agtagccgac aatctgattg attggattgt agcctttttc ttccaaagcc cgataaacaa
     5881 tcgctaatgt ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa cgtacggttt
     5941 catctgtaaa acccatgatt ctacacctct ttcccgcaag gtaattccag cttaccttct
     6001 acacccttat tttactcgaa gaaagggaaa actacaaaca aatattccaa acttctgaat
     6061 aaattaagaa acacgatttg tctgattttt ccttttttct gtggcttttt caaaagccct
     6121 ctttccataa agtatacgct atatttcttt gaaagtatgt ttttacacaa ttttttcata
     6181 aagttcataa aaaattcgaa ttgctgctac taatttttcg ttttcgttgc ttgccctgtc
     6241 tcataattga tggtgtagcc atcttgaaca ttgtagataa agacattgaa ttcaattttt
     6301 ttgtcttcaa tgctttctgc ttccaactgc acacctcttg ctaccagttc atttccctcg
     6361 aaatgaggag tgacacgata gcgtacatga tgatttgttt ccttgatata tgccgcaata
     6421 tcattttcgt gggcaagcat gtacggagca ttcaacgaac gtgtccctgt cattaagttt
     6481 ttaatgttat tattttgacc agtcaactgg aaaccaatca aatggctgcg gttgtataac
     6541 cattgtccgt cacttaattt cttttgtttc caacctgtcg gatcaatata caaaggttct
     6601 ctcttttcag taggaaacaa gtcttttccc aacatcgcat tggcttgtcc tactctgttc
     6661 aatttgtcta tatttgaata gctttcccat gtccctttgg aaagactaag atcctctttt
     6721 gtaaactccg gctgattccc attgatttct atttcttgat gttttccatc atattctggc
     6781 acatccgcta cttgagaagc ttgcggtgtg gaatcaaaaa tggttgtaat ttgttggaag
     6841 atatctgaat ctttttgtgt ggtggaaata ccgagtgccc ctgcaatcac agcagcgacg
     6901 atccccacga tcgccttggt tgtttttttg ctcatttctt tactccttta ctaacagttc
     6961 cctaaatctt aacatatgtt cccctataaa taaatactgg aaagaaatag aaacagtaaa
     7021 aaaataaaac cagtttttag acggcttaag aatacaaaaa aagagacgga ttcatcgtct
     7081 ctactcaatc acaaaacact atttacaagg tatttattat cattcaaaaa atttttctta
     7141 atactcaaaa ctaaacggct tctgtacgac ctcttggtaa acagtgttca aaagcttgca
     7201 tctgcggtaa taagtccaac caagtcaaaa acataagaag cttttttgct tgactattct
     7261 tattacttga tgcagggcag ctttttcaca gcctctttta agcgggtgac gggaatcgaa
     7321 cccgcgacat cagcttggga agctgggatt ttaccactaa actacacccg cgatacaaaa
     7381 acagtatacg cctctttcac tccttcctca agttttttta ttctttacgc ttccgatatg
     7441 ttttgtagta aaataaaact aatcaatcaa ttattggaga agatgctatg acgggaaaag
     7501 aattagatct gttgctggac acttgtttac tcgctgggaa aatcatgatg gaaagcaatg
     7561 cggagatgta tcgggtagaa gatacgatga gtcgaattgc tttagcttct ggaaatttcc
     7621 gtttggtcag ctatgtcact caaaccggtc tctttattgg tcttgatcgt acctcgacca
     7681 tccgaatgga acaaattacc aaccgttcga ttaatttaga aaaagtagta aaagtaaatg
     7741 atctttcacg taaatatgtt gcttcagagc taacattaga agaactttac tgcgaactgc
     7801 aagcaatcga ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag
     7861 tgatcagtgg aacaattatg gtgttatttg gcggaaagat gaccgattta cctgtgactt
     7921 tagtcattgg aggattggga tacgcagctt atctatatag tttgaaattt tttagaatca
     7981 aatttttatc ggaattttta tcttctttat tgattggcat agccgctatc tacagcgtac
     8041 gttttggctt aggaagcaat caggatctaa tcattatcgg atgtgtgatg cctttagttc
     8101 ctggagttca aattacgaac gctatccgtg atttgctagc tggacattat gtctcaggag
     8161 tttctcgcgg aacagaagcg atgatgactg ctacgatgat cggttttgcg attgctttta
     8221 ttttccagct tttttattag gaaaggaaca caagtatggg gacgatattt attcagtttt
     8281 cttttagttt tttagcgact gcggcatttg ccgttattac aaatgtacct aggcgttcat
     8341 tgatttgttg tggtctttct ggtgcttttg gctggatgat ctactggatc tgtgttcaaa
     8401 taggcggatc tgctgctttt ggttcattgc ttggttcttt aggagtagca ttcatcagcg
     8461 atcttttctc taaacgtttg aaaatgcccg taacgatttt caatattccc gggatggttc
     8521 ctctagtccc tggtggttta gcttatcaag cggttcgaaa tctcgttact gaatcctatc
     8581 gagaagcggc tcattatgcg gtccaagcaa ttatgattgc tggagcaatc gctttaggtc
     8641 ttgtattatc agaagtcttt aaccataaca tccgtaattt caaagtaaag cgtgacgcaa
     8701 tcatgatgaa acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg
     8761 tgaaagagac gttcagcctt gagcgatgac aaaatatctc acaaacattg cgacaagtaa
     8821 ccgcaggagc aataagaagt aattatgagt attaaaaaaa gttttgtaaa aatagcttct
     8881 cacattttta cataaaattg acttaatact gagaaactga cttttgaaca ccctctatct
     8941 gtaaaaaagg ggctgcgaca tgatttttgt cacggcccct cttctttatt caaatttttt
     9001 ccaaaacgtt cgttgataat aaacgatcca tagagcggaa agtattagaa ctaaaccgat
     9061 cacaggaata ttcactaacc aaaatggata aaacaaagcc aacattccgt ataagacgat
     9121 aagaataata ctgatgatca atgaacccat catagttgcg actaaaccca aacttcctga
     9181 acctctgtta aataattggt taatattggt ccagttcagc agcagcaacc gataatctct
     9241 agcgaaataa cgtagtgaca ataagtagca gccgaataaa gaaccaaata gcaagctagg
     9301 gataaaaaac aaaggcagct gaaaagacag tccgcttaat aatgcgatcc caccagttaa
     9361 aaaactctgc aatatccatc cgaatctgaa cttttctttt aaatattggt tcatagagat
     9421 tggcaaagaa cggataaaca gaaaattttc ttgatccaaa gaaataagat tgctgataaa
     9481 agaagtctga ttaactgtta agaaagccaa tgcgatccct accaggaaaa caacaccgat
     9541 taacttatta tctaaatcag ttaattttat ttcgcctcct agggcaaaag caagaataaa
     9601 aatgattgga gttaacaagg aagaagaaag aacttgcata atcaaattag gttcttttac
     9661 taattgagca ttgtaactaa ataaaagctg atgcacattt tggttctttt tatgggttct
     9721 ttttattttg aactcactgt ttgaatcagt cagttgctct gtaagctttg gtactatcag
     9781 ctgttttaca gcaaggaaaa atcccagagc cgtgatagct agtattcccc aacccaataa
     9841 accttgttga gaaagtggct gactggcagc atcatagaat ggcaataata tcgaaatcgg
     9901 atgccgatct gttaactcat tttcaatagt ccccgtttga tgattcatcc acatgattcc
     9961 agcaaatgcg atcagcatcg agctgccaag caagatacta gaaactgttt ttctatgttt
    10021 tttaaacact gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat
    10081 aatcaaaaat agagaaaaca aaaggattga gataatgata gtaagcaaaa tccctagatc
    10141 agctcgccat ccggttaaaa taaacatgac tagaagcgga aaaacaaaag gaatgatggt
    10201 caaagcaact accaaaatct ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa
    10261 aaatacttgc aggtcatttc cttcaaaaaa gatattataa atgactgata tactttgagt
    10321 taaacctaac ataccaaaaa gtgccacgta ataagtaaag aaacctggca atttaacaaa
    10381 gttcatagca aacatagcaa atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt
    10441 ttgagacaaa agatagatag ttaatcgctt tccctttttt cctttctctc ttgctttatt
    10501 cgtgagctgt ggattaacat ataaaagatt aactcgaatt aattctaata attgtcgctt
    10561 atacatacgg tccatctcct gtcgcttgcc ttccagccat tttcaaataa atcttttcta
    10621 gcgtttcatc aggtgagcca ttcaataatt cactaactgc cccctgataa atcaattcac
    10681 ctttcttcaa aatagccagt ttatcgcata actgttgtgc ggtatccaat acatgtgtag
    10741 aaaaaatgac tgtttttcct tttgcggcat gagccttcat catttctttc aaatcaaatg
    10801 ctgcttgagg atcaagtcct tgcaaaggtt catccaatac ccaaatatct ggatctggca
    10861 gcaacgtgcc gatcaaaatc gttttttgac gcattccatg tgaaaaactt gcgattgttt
    10921 cattttgatg actatacata tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt
    10981 tttgtgtgtc taattcatag gcagctgaaa tcaagtccca atattctcca gccgtcaatt
    11041 gtaaaaaaat atctggggaa tcaggcacat aaccgatttt ttgtttgata ctcaatcgat
    11101 tttccgtcaa tttcattcca tctactaaaa tcgttcccga tgatggttca ataatactaa
    11161 ctaaactttt aatcgtagtt gattttccgg caccattatg tcctaaaaat ccaaaaattt
    11221 ccccttgtct tatcgagaga gacaagtctt ttaatgcttg ttttttcccg taacttttac
    11281 ttacattgat gaattcaatc atttctgtac tccttttgtt tgttattact ttttattgta
    11341 cccttcgtta ttggtggagt aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg
    11401 actaggctgc tacttgggtt gttctcttct acaggaaaac actcttctaa caggtaagat
    11461 atgagtcttt gttttcttta aaatgattca ttttctatct tttcttgcat aaaattcttt
    11521 agtttatcaa tacttctctt tcccatctct agttgttctc catttgcaaa tgttactatt
    11581 tcgtttactc tgctaataga tgtaatgttt tcagtattaa tgatataagc ttttaaatct
    11641 ttacataaat actcatattc gagtgatttt ttatattttt taattgtgcc gtttacgaga
    11701 atctcttcat ctttcaatac taggcctatt ttatttttat aacctggcat gctcattaga
    11761 taaaggattt catcgtaatg gatatagtga tcgctattac cataacggac agttgtccac
    11821 ctttttttcg tagcatgttg aacaggatag ttttcttttt ctttcaataa gtaactttgg
    11881 atgctttttt tatttttttc ataagtgaat tcttttatca aataaccact aggcgtaatc
    11941 tttcgattaa taacagttat tgctttattc ggatgagacg ttagataaac aattttacac
    12001 ttttcattct tttctctaag catttcgcct agttcgatac cgctataata ggcgttcaaa
    12061 tcgatgtcta ggaaaaaaat atctgaggaa tagatttcat tatttttaat gaattcaaca
    12121 aattgtataa cgttcaatcc ctgaacgatc tcaaatttcc aaggacttaa taaaattctg
    12181 gattcttcca aaatttctac tagcattttt tcaaaaacca aactatcatc caaaataaat
    12241 atacgcgtta ccataaaaag tacctccact cgatatttta ctatcaaaat catttcatac
    12301 aacttttcaa catacatgtt aggaataaat tgtaattctc ttcacaatct tttccaacac
    12361 ctttgaaacc gatatcaaat actttcagga tgctgcttct aaaataaata tcaaacaaat
    12421 gaatacatca tgcaaaaaaa gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct
    12481 atgtttattt attttgttct tttaaaaaga acgatacaca tactgatttt tcggagcaga
    12541 gactttctca gcgtttgtaa caactacaga tgggatttca cgcttgaatg gttgataata
    12601 gtctgattgg atggttcctt ctattttata ccattcatca tttttaaccg ttactccttc
    12661 cggcatatgc gtcaacaaac caaagactcc tgaatctgcc acacaatgga taatgccgaa
    12721 tcggaacaag aagatatctg tattgctatc gttcggtgtt tggtaaacaa atccttcata
    12781 gctgattttt ttgccaatga attcgctagg gtaattataa attagctcca taacttctaa
    12841 atagttttca tctgtgatct tgatagtttg cttatcccca tacttttctt tcaactcatt
    12901 catttggtct aagtagtctg atttgttgaa ataaatactg gtatccggct ttaagtattg
    12961 tgtctgcatc tccggatccc ctactgattc tttacttact gggaaattaa atcctttggc
    13021 ttctacgatc gtagtgtcga gactaactgt tggaaacaac gtccccacga ttactggcaa
    13081 agctagcagt acataagcga ttccacgttg ataaggtttt gacaacccat ggtcatgatc
    13141 gtgctcgtgt gccttttctt ctgcttttcc gtttttgacc cacaaataca gctgaactaa
    13201 agccaaaaga gcagataaaa tcattgaaag aatagctaag tagcgataat ggacattgat
    13261 atattgatcg agtttgccag aaacttgcaa gtacatcatc aattcgaaat acccagataa
    13321 aatcaaaaac cgcagcatat tttccctcct aaataaataa tgtgaatcca ataacgctaa
    13381 gggcaatcag tccgatcagc gataaaataa agcgtcctgt aaaatatcgt ttcatcatca
    13441 gtagattttt gatatcgacc attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc
    13501 cgaacagact taacaaagaa gatcctataa acgcatctgc ttctgagcaa agagacatcg
    13561 taaaggccaa taacagcatg atgataatgg ctaaaatctt tgttgagccg agcgttagca
    13621 taattctagt tggtaaataa gtctgcatcg cagctgcgat taatccgcca atgatcaaat
    13681 aacgacctgt atcaaaaaat tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga
    13741 tcagctgatt ttttggttca ttcttctcac tttgttcttc ttcatggaca tgatgatggt
    13801 gattttctgc tactttagac gtgatttttt ctttcaatac agcgtctttt tgaaaatagg
    13861 caagccagac accaatcaca agggcaacaa aaaagctgcc tatcacccgc cagcttgcca
    13921 tctgccaaga attgccaaat gcgataaaag tggaaaaaat cacgattgga ttgatgatcg
    13981 gagcagtcag cataaaagca aacgctgtgt gaactggtac gcctttttta acaaattgat
    14041 gaacgatcgg tacgatgccg cattcacatg aaggaaagaa aaatcctaag atcgaaccag
    14101 taaaaataga gaagatacga ttttcaggaa gccatctctt cacacgttct ggggtcaaaa
    14161 atacttgtaa ggcgcctgag ataagacagc ctaataatac gaaaggtaat gcttcaataa
    14221 cgatcgataa gaaaatcgta ctcatctgca aaaccgaatt tggaataaaa gaaaacatga
    14281 gtttcctcct ttttatttct agttcattcg ctttgcaaat atacgctttt attcaaaaaa
    14341 aacaagttgc tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga
    14401 ctttttctgt tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg
    14461 agaataaaaa tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca
    14521 tccgttgccg tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca
    14581 cgactagagg aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa
    14641 acaccagaag tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat
    14701 caaggaaaag aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta
    14761 ctagatgaac tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa
    14821 taatcgttgt ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt
    14881 caatcgcttt ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca
    14941 agaaatagat caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg
    15001 acggagcatt caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta
    15061 actgattgac tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt
    15121 gttggatctt gatttgttct gacatatctt ccattggata aacggggata ccgtctacga
    15181 tgcgcccaac gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt
    15241 gatggaattt gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat
    15301 ttgtcaattg atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat
    15361 aaccataccc acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac
    15421 tatctacttg aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat
    15481 ttaaaatcct taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa
    15541 tatgatcttt cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc
    15601 atagcagtat ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct
    15661 ttattcattt atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta
    15721 ctaattgaaa aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat
    15781 gatattatta caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa
    15841 catacattta gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg
    15901 aaaatcgact ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc
    15961 taaaaataaa acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga
    16021 aacagtttgg aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat
    16081 gcgcgagcta gagattttca ttagcgaagg tacgccagac acacctgctt atagttctta
    16141 tttaaaagaa tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga
    16201 aaatgaaatt cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa
    16261 aatcgatact ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca
    16321 acaccctgac attttgattc tagacgaacc aacgaaccat ttagatatcg agacactttc
    16381 ctggcttgag aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg
    16441 ttatttttta gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca
    16501 ttacaaaggt aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg
    16561 gaaggcttat gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa
    16621 ccttgttcgc gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat
    16681 ggaccggctg gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga
    16741 aaagccttct ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt
    16801 caccttatct gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg
    16861 tccgaatggt atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat
    16921 ccgtggaaaa gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc
    16981 tgatctgcat acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc
    17041 agaaaaagag atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa
    17101 accaatccct ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat
    17161 gaacaaagaa aattttttga ttttggatga accaacaaac catttagata ttgacaacaa
    17221 agaagtatta gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga
    17281 ccgatatttc atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa
    17341 agtctatcta ggagactatg attattattt agagaaaaag caggaagaag aagaaatcgc
    17401 cgaattgttg gcacaagatg agccagaatc tgctcctgct cccacgaaca aatctgatta
    17461 ttaccaaagc aaagaacagc aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt
    17521 tgaagaagaa atggcgcgga tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc
    17581 gccagaaaac ctggaagatc acgtaaaact caatgagctg aaccaagatt tagaagctgc
    17641 tagacaagat caagaaacaa aattgacgga atgggaagaa ctaagcttgg aattagaaga
    17701 atatgaaaat taacgaaaaa accaaaacag aaaccattca tgctgttttg gtttttcttt
    17761 ttttatttta tcaaacaaaa attccatatt tacctttttc accaataaga agaccaatta
    17821 ctagtcattc tcactaaagc ttctaaataa tagtaatcac cccaaataca aggtgtattt
    17881 accccattat ttttgggctt attataaaca gactgtgtta aaaagccatc ttcttcttcc
    17941 gttgtagcat atctttcttt caattgtcgc ataattttcc aaacagcaga ctggtataag
    18001 ttcttttctg ttttgtctaa tagacctgat tctattaatt ctaataatcc acaaaccatg
    18061 atcaccatac tagatgtatc tttttcttct ctgctagaat catcaaagat caaatcccaa
    18121 taacaaatgg tatcttctgg caaccgattc ataaaataat tggctaatat tttgcttaaa
    18181 tcaaaatatt tttgttcttt caaataacga taacttaatg caaaaccgta aatcccccat
    18241 gcttggcctc tagcccaaca ggaattatca ctataacctt gatgcgtact tccatatctt
    18301 ggttttccat ctttgacatc catataaaat gtatgataag ttgaaccatc ttctcttact
    18361 aaataatctg ctgcattttg aatatgagcc tgagcagctt ttttatattt ttgatttcca
    18421 gtttcttcac tcgcccaata caataatgga agattcatgc aacaatccat gatcattcta
    18481 ccttgttgtg aaggatcact aggatctccc caagcttgta taactcctgc cttctcccaa
    18541 aaacgctgaa gtaatgtttc tgcagcatca attgaagctt ccttcatttt tcgatttccc
    18601 gttattttat aatctgctac ggttgataaa gaatacaaaa aaccaagatc gtgatgatta
    18661 gtgataattt gacagtcaat cctgtggata aaatctatat ctgctttttc agctgtttgt
    18721 tgaaataaag gatcacccgt cttctcaaat aacaaccaat taattcctgt ccaaaatcct
    18781 tctgtccaac caatattgtc tcttaatgga taacgtcctt ttataggaca ctcgcttggg
    18841 aattgatact gactgttact gatacgtttg attaacctct ccgtgtgtct gcgacaacgc
    18901 tcgatctcat tattccattc tttttccatt aattctttat aacattcatt tatccatttt
    18961 tctggatgta ttatctcttc aaacatcact ttatcaagca tttccccacc agcctttagc
    19021 ataattttag tcttttttta ttgtttttat tttggatagt tagcccggtt gtcccatcaa
    19081 aatcaatttt gggacgattg ttccatactt ctaaactata atcccgatct tgatgagcaa
    19141 ccactcctgt tccgagccaa tgggttcctt ttccaaatgt accctctaat ccaggaataa
    19201 aagtgttttc ccatgtcatc aagtttgtat tgggcaaacc cataatagct gtggattttc
    19261 gtttaatagt atcgtcactt aaacagataa tacctgaaaa gccagcttca ctcaccctat
    19321 agttttcctt gtcttcttct atgattattc cttctttctc aggagaacat tcaatagcaa
    19381 accctgtctc atatagaaca tattctgtat ctaattctac tttgtgtacg cgaatctgcc
    19441 agccttcaaa aggaataagc caagttctga tttttgcctg taaagtaggt tcccatgtag
    19501 aggcaagtac ctctttctta catacaatgt ctcgtacatt atttcgaacg atgaactgtc
    19561 cataggtttt gatgctctca ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa
    19621 tccctagcat tgaatccatt gccatttctg ctttcaattg attcgctcgc ggtatcgaaa
    19681 aaccaaattg actagaataa gcaaatttta aatatttttc attggtcaat gtatggaaat
    19741 ttggccctaa ttgtcccgca ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca
    19801 aattggcttc tgaaagaagt ttagttcctt cacgaatagg cataggcatt tctttagccg
    19861 tccaatactc atggtcttca ggtagcgcca acaataaaaa aatcttattc agccataatg
    19921 gagataacgt actattatag ggttcagtca atgtcaattg agggtaggta taaccaatat
    19981 tcaggatacc tttttcatca aatatttctt tttgcatcca ccatcttata ttccgattga
    20041 ctaacccctt gatttcacct aatttaaaag gcaaaagatc ggcccacaac atagctgacc
    20101 aaaagcttga aactgcaaaa cgataaatca tgcttcgacc aaaaggcaca tttgcaccat
    20161 cttccgcaaa aaaatgaata tagtctctag aaaaatcaat agctcggttc ttatatatct
    20221 ggctaatggg cttttctggt tctagtacgc tataaatcaa accataataa tgaaatgcaa
    20281 aaggattata ataatcttgg cgacctcttg aactgtctcg ataccagcca ttttgaagat
    20341 aacaattttc tatattatca aatgcttctt tcagtgctga ttctttaact ggataaccaa
    20401 atttttggat aattgttccg acaattatct taaaaaattg ccaattacca tctgcacatt
    20461 catattcaaa aatttgcgag aaccaatgaa taatctggct tttttctcct gtatctaatt
    20521 ttttccataa taattgttca tgatgatata acgctaataa cattgcgggc atttctacca
    20581 tacgctggtc tctttcatgt atttctcccc aataatcggg atgggaagga tctgttccag
    20641 cttttattcc tgatagaatt aatttcaacc atcgttcatc taattggtca attgcgggcc
    20701 ccgctcccca taaaacccga gagaatcctt ccattcctgc aattttattt ccatatccta
    20761 ccccttgatg tatataatca atttgtgcgc cacttgctcg ataaaacggc tggagtggtt
    20821 gatttatctt tttaaataag tcaaagtaat cctgtctggt ccttaatagt ggtactggat
    20881 gtttcattgt attttctcct ctttttacta ccaaatagct aattccgact gttctaatcg
    20941 aagtaagcct tcaataaaaa aataatcaga atagatcagt ggcacattat aagtcagttc
    21001 tctatggtat gcttctgctc cgcctccaat gatcccatct tcatccatat tccaatttgc
    21061 atagtgcttt tctgtctttt ggagtatatt gatagcagct tcatgataca tttttgatcg
    21121 tacagaggaa acatatcctg ctaattccag aagaccgcat gcagcgcata ctccagcact
    21181 ggtatcaata ttcgaatctt ttacagcaaa atcaatgaga ggaatataac cagttttttc
    21241 gattttctga ataaaattta ctgctatctt ttctgctata gctagatagt acggattttt
    21301 tgtgtaacga taacttaacg cgaatccata taaagcccat gcatttcctc tgctccaaca
    21361 agaattctca ttgtatcctt gcccgccaag taaccgaata tatttccctg attctgcatc
    21421 aaattctgcg atatgcccgg ttgatccatc tttcctcact agatacttag ctgttgtatc
    21481 tgcatgctgc cgagcaattt tagagaacct cggatcacca gtttccctcg aagcccaata
    21541 cagtaatggt aaattcatca tactatcaat aatcatccaa ccaggtttat ggtcattcca
    21601 tgctactaaa tagttccctg aaatattata gcgtccagca agtaccgtag ctgcatgtaa
    21661 ccctagttgt cgactatgtt tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa
    21721 gaataaaaaa cctacatcat gatgaacgtc tgaaaaatgg tacagggcag attctaataa
    21781 ttgttcttgt tgtattgctg cctctttgaa cttttctcgt tttgtcttgt gaaagagctg
    21841 ccataaaata ccactccaaa aaccattggt ccaccagtcg attcctctgt cctttattac
    21901 gtctgtatat tttccgttgt aagaaatata tggaatttta ttccccattc tgtctaattc
    21961 cgcatctatc ttttcagaag ctacattcaa aatttttctt gaccattcct tcgtctgttc
    22021 attcatggat atcccgccat tcttttaatt tttatgaatg tggagttgct cctagaacga
    22081 actagatggt taacattcgt tatttttttt acttatttgc agctccaatg tcattctcct
    22141 taaatttaga ctgcgcccac acataaatca agaccattgg gatcactgaa gttgctaaaa
    22201 ttagcataat tgcgccataa ttaattggct cacctgtact agaaaccgtc atatttttga
    22261 taattaaggg aaccgtatat ttatctttat ctgaaagcat gactaatgga aatagatatt
    22321 gattccaact tcccataaag ataagtaaac cactagtaat aatcgtaggt gataaaatcg
    22381 gtacagcgat tttcgtaaaa atcacccact cattggcacc atcgattctg gcagcctcca
    22441 tcagtgctgt aggaaattga ctgctatatt gtcgcatcat aaacactgaa gttgtactgg
    22501 caatcgaagg gatgatcaca cctgcataag tattcgataa tcctaaacga ctcataatgt
    22561 aaaataaagg aatcaatgtg gtaaaaaaag gcaccatcct agatagcata actgtattga
    22621 acaacagttt attccccata aaacgatact ttgctaatgc ataacccgcc attgtagaac
    22681 tggctgtagc tgaaactgta ctgattacag caataacaat cgaattccaa ataatcaagg
    22741 gataattata tctcgccatg atggcttgcc agttttttaa tagcatatcc ccaaacttga
    22801 aattgattcc gccacttaaa atttcttgat tactttgcgt tgaagctaca atcatatata
    22861 aatatggcat caaaaatatt aaggcaccaa acacaataat gacgtacgta aaaattctgc
    22921 tttgtatctt ttgtttagtc atttttatca cctccgaatt taaattgaat agccgataat
    22981 ataacagata caaacaccat cgtccaggca atagcagagg cttttccaaa atcaaccatt
    23041 tcaaaacttg ttttatataa attgactcct acgaccaaag ctgaattttc tggtccattg
    23101 gaagtcccaa agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa
    23161 actgtggcca ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga
    23221 aatttcgatg ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga
    23281 ccagctaaaa ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt
    23341 gatattcgta tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc
    23401 aaaaatccat tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc
    23461 accgttgatg taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac
    23521 tgtgctgaat tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat
    23581 cctgccatga taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata
    23641 gctagataat tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa
    23701 gcaaacgaga taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg
    23761 accaatccat aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt
    23821 attgtcttgc ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc
    23881 accagttaat tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt
    23941 ttcaaaatca cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc
    24001 tgaataagtg gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc
    24061 atagtatttt tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc
    24121 caatgagtta gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt
    24181 cagcataaat gtaagaaatt gttgtgccac ttgtggattt ttggcatctt tagggacata
    24241 aaaacttgat cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag
    24301 tggagccatg cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata
    24361 ccagccgcca gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc
    24421 gtagaaatgt acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc
    24481 ttctttagta ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc
    24541 tagcatgttg ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc
    24601 agttttttct tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc
    24661 aaaatctaca cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa
    24721 atcactagcc caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt
    24781 ttgatcataa aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa
    24841 agcatcttga aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc
    24901 cggcatatct tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga
    24961 agctacattc acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc
    25021 agcattttca acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc
    25081 tttttttatc attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc
    25141 acttaagctg aataataggg taatgtttag caaagctatc accaatttgt atcttttttt
    25201 catgtctatc ccttctttct tttcaagttc ttatataaaa cgctttcact tatgtatatt
    25261 aacggaatta gtaacacaaa tatttatata aaaaaatatt ttacttgtcg ttttggcttt
    25321 tcatttttgt tataatagtc attaattata aatagaaagc tttttatata aaaagagaaa
    25381 ggaaagtggt tcttttgtta acaaacaacc aatcctccaa tcttgtcaaa aaaacactaa
    25441 cctattccat agatacccat cttcatttat ctgctgctcc ctattttcga ctactaataa
    25501 atgaaagcgc taatttatat gctgaatttg cacataaatc tatagaacta gataatcctt
    25561 cccttttatt cattgataaa cagcaagaag tccaaatctt ttctactcaa aaggaaacac
    25621 aaacttttat cattatccag ttttattttg atgagcaatt attagcacat attacgggac
    25681 aatctttttc tatagatcaa gaaataacat ctatcattac ttttctgaaa aatgaacagt
    25741 tcaaaattga ttatctaaaa catagtaagc cagctatttc ctatgatcag gaaatcaatt
    25801 cttttttcat actatgccaa cagatgagca ttcatttgct ttccttatta gttacttatt
    25861 gtctgcaaaa aattcaattg tcttccttac caaggcatta tcaacaggaa cttgctttat
    25921 tgacaatacc ttcaaatgag aaagtcattc ctaaaaaaaa gcatattctt tattcagatg
    25981 aattagttga tccaatcata cgatatttag aagaacatct ttcagaaaat tttttgcttg
    26041 aacaattagc ccaaattttt tttatcagtc cagccagctt aaaaaaacat tttagaaaag
    26101 tgaatggaaa aagtgttatt agctatttta aagaactaaa aatggaaaaa gcaaaacgtc
    26161 tcgtgctaga aaatgaatta acctatacag aaattgcaga tatattagga tttcattcga
    26221 tccatcattt ttcttcagct tttaaaaaat atacagggtt atccccttca aagtattatc
    26281 tatccttaaa atagaaaaaa atttttaggt tttatccaca aattatttct ctatattttc
    26341 tgtctattca aaataaacaa taattttccg gcacaattat ttgctgtttt ttaccataac
    26401 atagtacgat caacgtagaa aaggagatgt ttttatgaaa gttgttgtca ttggcggtgt
    26461 agctggcggt ccttcatttg ccactcgttt ccgtcgattg aatgaagcgc acgaaatcat
    26521 tatctatgaa cgcggagaaa atatttctta tgcaagttgt gctttgcctt attatttagg
    26581 aggtgtgatc actgaccgtg actcgctgat cgaacgtaca ccagaaatat tgaaaacaaa
    26641 aaacaacatc gatgtcttta ctaaacacga agtaacagca atcgatcctt ctactaagcg
    26701 attaacagtt aaagacctat ccacaaatga agaaataaaa acagattacg ataagttgat
    26761 catctcttct ggtgctagac cagattatcc ggatattccc ggagtttttg aagcagaaaa
    26821 cggctttgtc ctccgtagtg tgacggatgc ggatcgaatc aaatcgttcc ttgaagaaaa
    26881 aaatccacaa catgtcgtca ttcttggtgc aggtgttatg ggtctggaat tagctgagaa
    26941 tttcaagcat cgcggcttag acgtgacttt aatcgatcaa ttgccacaag tcgctttccc
    27001 ttatgatcca gaaattgcta atttagttta tgacaaattg ctgaaagaag gattagccgt
    27061 tcatttagaa acaagagtta ctgagatccg tgataaaggt cgagaaatag tattatcaga
    27121 tggctccgtc ctttctgctg atatgctaat ttttgctgtt ggtgtttctc cgaagaatga
    27181 agtggtgaaa gcaaccggca tacaattatc agatacaggt cagatcattg ttgatgacca
    27241 gttacaaacc aatctgccgg acatctatgc gattggcgat attatcgaaa cgactagtgt
    27301 agtgactggt cagccgatcc aaagtatgct ttccagtgcg gccaatcgtc aaggacatat
    27361 gctggcagat attttaaatg gtacgcctat gcgctatcgc ggatatattg gtgcaggtgt
    27421 cgcaaaaatc tttgatcata cagcaagtta tgcgggaatg acagaacatg aattaaaagc
    27481 atcaggcata acaaattata aaactgtttt tatcactccc tttgaccatg cctatttcta
    27541 tccagaagct acaagattaa atctaaagct gatttttgat gcagatagcg gtcgtatttt
    27601 aggtggacaa gcatttggag aaaaaggtgt cgataaacgg atgggagaac tttctgtagc
    27661 aatcaccgga aacttgacag tctttgattt gcctgatttg gagttgcctt actctccacc
    27721 gtattctact acccgtgacc cgttgaatat agctggttat gtcgcaatca atcaaatgac
    27781 gaatatcgta gaaacgatca aagcaagtga tatacccgaa aacgatttga aagaagcgtt
    27841 ctttttagat atacgtgaac ctaataaagc accttctggc agtatctcgg caacaaaaaa
    27901 tattccaatg aatgagttac gagatcgaat caatgaaatc ccaaaagata aaaaagttta
    27961 cattactttc agaagaggat tgaatactta tacttctgcc cgaatcttgg caggtttggg
    28021 tatcaaagcg attttgattg aagaataaaa aaacgaccgc ggtgggtagc cgcggtctag
    28081 gagttatttt tacttgagga gtaaaaatga aaaagtatat agggttgttg gttggtatgt
    28141 atatagaata gaagataaat gtgaagaaat tgtgcattat ttcttcacat ttattaggtt
    28201 atagtttaag aaagtttaaa cattgcgcaa tttaattatt ttagctgttt cttttcataa
    28261 aaaaacatgt tgtgactctg agacaaatca tagtccatag tgatgtctct agagtaacta
    28321 cgcctaatat tacgaggcat catattaatc tatcagcact agtacctaaa ctagcatccc
    28381 tttcgaatat tttttcttat ataataaagt ttgctttcac tcctctaatc tcaccaaatc
    28441 gactggcttg cgttgtgtga tactaggaac tgattggttt agaacgactt ttctattgta
    28501 atcatatttt tcgccatcct cgttgtcttc taatccttca atgaatgatc cctaatgtgt
    28561 gtattaaata atttaatttt tttgttccta tttattt
//
LOCUS       DACEKH010000027        27797 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEKH010000027 DACEKH010000000
VERSION     DACEKH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.027"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(235..1179)
                     /locus_tag="IW662_001113"
     CDS             complement(235..1179)
                     /locus_tag="IW662_001113"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0865951.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1475..2419)
                     /locus_tag="IW662_001114"
     CDS             complement(1475..2419)
                     /locus_tag="IW662_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0865952.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2640..3854
                     /locus_tag="IW662_001115"
     CDS             2640..3854
                     /locus_tag="IW662_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0865953.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3876..5891
                     /locus_tag="IW662_001116"
     CDS             3876..5891
                     /locus_tag="IW662_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ0865954.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5974..7347)
                     /locus_tag="IW662_001117"
     CDS             complement(5974..7347)
                     /locus_tag="IW662_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0865955.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7373..7657)
                     /locus_tag="IW662_001118"
     CDS             complement(7373..7657)
                     /locus_tag="IW662_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0865956.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7659..9674)
                     /locus_tag="IW662_001119"
     CDS             complement(7659..9674)
                     /locus_tag="IW662_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0865957.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9853..10158)
                     /locus_tag="IW662_001120"
     CDS             complement(9853..10158)
                     /locus_tag="IW662_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ0865958.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10247..11278)
                     /locus_tag="IW662_001121"
     CDS             complement(10247..11278)
                     /locus_tag="IW662_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0865959.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11485..12360
                     /locus_tag="IW662_001122"
     CDS             11485..12360
                     /locus_tag="IW662_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0865960.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12512..13354)
                     /locus_tag="IW662_001123"
     CDS             complement(12512..13354)
                     /locus_tag="IW662_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0865961.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13446..15323)
                     /locus_tag="IW662_001124"
     CDS             complement(13446..15323)
                     /locus_tag="IW662_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0865962.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15320..16741)
                     /locus_tag="IW662_001125"
     CDS             complement(15320..16741)
                     /locus_tag="IW662_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0865963.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16744..20135)
                     /locus_tag="IW662_001126"
                     /pseudo
     CDS             complement(16744..20135)
                     /locus_tag="IW662_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            20392..21783
                     /locus_tag="IW662_001127"
     CDS             20392..21783
                     /locus_tag="IW662_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0865964.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21856..22503)
                     /locus_tag="IW662_001128"
     CDS             complement(21856..22503)
                     /locus_tag="IW662_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0865965.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22511..23197)
                     /locus_tag="IW662_001129"
     CDS             complement(22511..23197)
                     /locus_tag="IW662_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0865966.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23203..24276)
                     /locus_tag="IW662_001130"
     CDS             complement(23203..24276)
                     /locus_tag="IW662_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0865967.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24498..25172
                     /locus_tag="IW662_001131"
     CDS             24498..25172
                     /locus_tag="IW662_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0865968.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25177..26517
                     /locus_tag="IW662_001132"
     CDS             25177..26517
                     /locus_tag="IW662_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0865969.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26666..27652)
                     /locus_tag="IW662_001133"
     CDS             complement(26666..27652)
                     /locus_tag="IW662_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ0865970.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8104 a         5250 c         5045 g         9398 t
ORIGIN      
        1 tttgccgttg cggaaggatt ttttaattaa ccacagataa acattgataa cgattcgctt
       61 attgtaatca acagattaaa tttcatatgt tcatatgtac ggatacattt tcggaccgat
      121 taattttaac cattctgttt atttgcttac ctattagata tcaataaaaa actgcttaac
      181 agctatttca gctgttaagc agttttagga tttttagttc atattcattg tttcttataa
      241 tacagtgttc aatacttctc ttaaagcact tgttgatttg ctgaacattg ctttttcttc
      301 ttcgttgatt gataattcaa caacttcacg aacaccattt tcgtcaacga ttgatggcac
      361 acctgtaaag atatcttttt cgtcgtattc accagttaaa taagcagata caggcaatac
      421 agcttgttcg ttattcaaga tagcttttac gatacgtgca gtactcatac caataccata
      481 gtaagttgct tttttacgat caatgatctc ataagcagca ttacggactt tatcagcaat
      541 cacatctaat tcatcttggg caatacgatg gtctttttct acaatttcaa acactggttt
      601 accaccgaca gttgtatgtg accatgcagc aacttctgaa tcaccatgtt cacctaagat
      661 atagccgtgg acgctgcgtg ggtcaacttt taatttcaat gcgatttctt tacggaaacg
      721 agttgtatcc aatgttgttc cagtaccgat aacacgtgat gttggcagac cagattcgtt
      781 ccaagcgata tatgtcaaaa tatctactgg gtttgaagca acaacgataa taccgtcgaa
      841 tcctgatccc atgatttctt ttacgatttg gcgcatgatt gacgcatttg ttttaactaa
      901 atctaaacga gattgaccag gtttttggtt gataccagct gtgataacaa cgatatcagc
      961 atctttacat tcttcatagc cacctgacca tacagccaca ttttcatcgc cccaagccat
     1021 accatctaaa aggtccaatg cttccccttc tgctttttct tgattcacat cgatcaatac
     1081 taattcatta gagattcctt ggttgatcat tgcataagcg atacttgttc caacaaaacc
     1141 tgtaccgacg attactactt tacgacttgt ttttttcatg aataaacagc tccttttcat
     1201 aactatttgt tactttcatg aaaagtatag tcttgtttat tcattttatc aacatgttaa
     1261 ttataatagt tgttattata atttttgatg gaaacgaatt cctgaatacg cataacagat
     1321 aaatgacaaa tgtttacacc tatatcccct gataatacaa ccaaaagttt agaactgaaa
     1381 ataaatccaa aaaaattcgc tgcaacgata taataagaca gcaatcgcac ctaaaacaat
     1441 tttccttcat atgctttagt gaaagatttt cttcttaatg tttatttctc cattcattgg
     1501 gagtacatgt tgaatacttc ttgaataatt tgaataaaga agtcgtttgt ttatatccga
     1561 tacgattagc gatttcttcc aacgaaaaac tcgtttcttg aatcagttcc aacgcaaagt
     1621 cgaatttttt ctttagaacc agttcttgaa aggaagcatc aaaatacatt ttcaatttat
     1681 tactaagata gaccggattg tagccaaaat gagctgctgt ttcttttaac gtacaagaag
     1741 taaaatgctt atcgatataa tctaatactc taagcatctc attttctgcg catccatctg
     1801 ctgatgaaaa gctttgttgt tctaataaat gagtcaattc gatcagtaat agtgatgtat
     1861 agagatttaa cgaacggatc ttatgactgt atgtatcaaa ataaatcaac agcatatttt
     1921 caatcaaatg cgtcaaacga cttgtttttc cgccgcgaaa atgaatgaat tgtgtatgtt
     1981 cccctttttc ttttgatgca tttatcaaaa aagaagaaag tagattattt gtgcttgcca
     2041 aatgattcaa tatctctgta gataaggttt cttcttttaa taagatgttg attaaaatat
     2101 cctcttgccc tgtgtattcg attctttgag tagcttcttt atcaatgatc accacgtcac
     2161 tttcttccat ctctatcctt tctccattaa taatttgtaa acatgtaccg ctatacatat
     2221 aattgatttc aacaaaatca tgtgtatgct cgggaacttc cgcatatcga cggtgtttac
     2281 tgatatgaat atttccctgt tgaaatagat cccctcggtg gatttttggc ggtgtctgaa
     2341 gatggtaagc taagtttaaa tcattgatat tcattccttg ttcacgctgc agagtttcga
     2401 ttgattcttc gttacgcaaa aaaaatgtct aattcagcta gttccaattg actccctcct
     2461 ttttgtaagc ggtttctttc gcttattagt tcacgctgta ctgctattat atgcaaagct
     2521 atttttggtt tcataaagac tttctatggt tgggacatga acttcctgtg attccgctat
     2581 tgagagaata accacttcat actatatttt tgataagcgc ttacaaaaat gaaaggaaca
     2641 tgcttatgaa taaaaagatg atgaaatttt ctctgctgtc agtctctctt attttgactt
     2701 ctgctggtgc agtcacgtct gcaattccca gtatgtcagc tacttatgcc acgcgttctt
     2761 tatctgctgt tgaattatta acgactgctc cctcttttat ggtcactatt tttgttttga
     2821 ttagtagttt tattgctagg aaaatcggac aaaaacaaac tgtgatcatc ggattagtga
     2881 ttgcatcgat ttgtggtatc ctccccctct tctcacaaag ttacagtatt gtcttagctt
     2941 caagagctgg actaggtatt ggctttggat tgatcaattc tttggcagtc agcatgattg
     3001 ctgcctttta tcacggagat gaacgagcct ctatgattgg ttttcaaagt gcatttcaag
     3061 gatttggtgc agctttaatg acatttgttg caggacagct agtacagttc ggttggcaat
     3121 ataccttttt agtctacgcc atcagcttac ctattcttgt cttgtttgtt ttgtttgttc
     3181 ctacacctga aacaactgaa cttcaatcgc atggcacagc cccaacaaga caagtaatca
     3241 aaacgaatcc taaaatgatt agttatggta tttttctctt gctggtcatc gtggtatata
     3301 atgctgtatc gattaaatta gccacagtcc tgacgactta ttctctagga aacgaaacta
     3361 atgcagcgaa tatcttaaca atcatgcaat tggcaagtat ggctgccgga gtttgttttg
     3421 gctggcttca gacaaaagca catcgctata tgcttacact ttctttacta ctgatgggaa
     3481 tcggctttgt attgattgcc actgtgataa acctgtatgt gattgggttt ggtgcgatcc
     3541 taacaggaat ttctttttcg ttattcattc cttacttatt caaccaagta agtatccaag
     3601 cgcccaagta cgcggagagt ttcaatactt caatcttatt ggttggcgct aatttaggta
     3661 ctttcattgc accatatggt ttgctctttc tctcttttgc ttctcttggg agtaaaacac
     3721 ttgctgtttt cgtcaatggc agtatcttgc tgttgattag cgcgcttatt agccttctag
     3781 tagtacacag gacgaataga aagacagtga taagagtaga tgaagaaata aaagaacaat
     3841 tgacaacaaa ataaatttga tggaggtcgt gtaaaatgac aaatgaaaaa cataaagata
     3901 gatggctttg gtatcctgga gactttgaga tccgccatgg actgctacaa aatttccaac
     3961 gggaagaacg aggttttgac tggccagcgt actggtacat ggatgattgc catcggaacg
     4021 tcaagtttaa gagatattac tttcttgatc aaccttccat gtttaaagtg acgatccaag
     4081 gagttgggta tgtagaaatt aatggacaaa aacatccatg tggaaaatgg ctgacttgtc
     4141 cagctggtaa agcaaaaatt cgtatctttg taggacatac atcagggcta ccagcgatgt
     4201 ttatcgaggg agacgaagta aaatcagata ttggctggac agcaagtaat tttattgaag
     4261 aatatccagc tggctggagt ccgctttatg tcgatatcgc aaaagatcca aatcaaatct
     4321 attatcaaaa agaatgcgtc catccagtca ctgaaacgga gattggcgga ggaatcttat
     4381 tcgattttgg gcgagcagtg aatggctgtg tagaactaat atctctggat gaccatatcg
     4441 aatctatcac tctatgttat ggagagtccg atgtagaagc tatggatatc gattattgct
     4501 actataagca aataggaatg acaagaaaaa tgaaagctcg taaacgcgct tttcgctatt
     4561 tgtttatacc agaggtttct actggaatgg ttcaacttca agtttttcat gaatttttat
     4621 ctctttcgaa acaagctgct ttttcaagcg attcttcttt actgaatcag atctggtcag
     4681 tatcagaaga agccttcaag ttatgtagtg atttgttttt tatcgacggt atcaaacgag
     4741 atcgctggat ttggtctgga gatgcgtatc aatcattcat gatcaatcaa tatctttttt
     4801 ttgacgagga aatcaataaa cgaacgatat tagcattaag aggacaaaat gaaatcaagc
     4861 agcatatgaa tactatcgtt gactattcga tcttgtggtt gattggtatc gaaaaccact
     4921 atatgatgac gaaagactat gaatttttac gcatcgtcta ccctaagatg caatcattaa
     4981 tgagatactt gatggagcaa acgaatgaat tagggtttat ttatgggaga gcaaaagatt
     5041 ggatatttat tgattggtct gaaatggata aagaaggaac gcttgctgca gaacaagtct
     5101 tattactgaa agcctatcat tccatgatca cttgcggaga tatcttagct gaaacatatg
     5161 gagagaacga aaattatcat actcatctct atcaagaaaa atatcaacta ttgaaacaaa
     5221 acctatttga ctacttttgg gatgaagaac ttggcgcttt tatcgattgc tatgaatctg
     5281 ggagaagaaa tgtgacgcgt catgcgaata ttttcgctgt tttattcgat tttgctaaca
     5341 aagaccaaca gcaatcgatc ttaaagaatg tcttattgaa tgacactatc acacagatta
     5401 ctactccata tttcaaattc tatgaacaag atgctttatg caaactcgga cagacgaata
     5461 tcgtctacca aacgattctt gattattggg gagggatgtt atctcatgat gcggtaactt
     5521 tctgggaaga atacgaccct actcaaacag gaaatgaaaa gtacagtatg tatggagatc
     5581 cttatggaaa aagcttatgc catgcatggg gagcaagccc aatctatctt ttaggacgct
     5641 attttatcgg tttgtatcca acaaagccag gttatgaaga atttatgata caacctaatc
     5701 tagattcatt tgaaacactg aattgtacgc tacctatcaa agatggaaag atttcgcttg
     5761 aaaaaacaaa agataagatc gtgattactt ctgatagaac aggcggaaca gtgaaaatga
     5821 atggtaaaag ttatcctctt ctagcaaaac agccacttgt cttaacaagc tattcaaaaa
     5881 taaatgcata aaacaaaaaa ctgcttgcga catatcagaa gactttaaat cttcttctga
     5941 tattgtccaa gcaattttta ttttgaagtt tgcttaattc tgttctttag aaacaatgtt
     6001 tgttcttttt ccggcaaaaa gaaagctaga taatacgaca agtaaaaaga aagcgatgat
     6061 tccaactgtc aaggctattt gaccgtattc tcctagtttc ccaagtacaa gtcctggtac
     6121 tagataatca gtatcggcaa atgtagaaga agcgatttta agatctccta atactggaag
     6181 gataaataat ggcagccaac taatcaataa gctattgaaa gcagaaccta ataccgcccc
     6241 tcttctaccg ccagtcgagt tgccgaagac accagaagca gctccgcaga agaaatgtcc
     6301 tactacccca ggaatgataa ctgtagtttg caagcctatc atagcaaaca tggatagtgt
     6361 tcctacaaag aagctaacaa aaaagccaat taatacagca ttaggtgcat atggaaaaac
     6421 gataggaaca tctaatgctg gtttagaatc gggtacgagt tttttagcaa tcccttggaa
     6481 tgctggaacg atttctgcca acaccattcg aaccccttga aggacgataa caaacccagc
     6541 tgtgaatgtt ccagcttgga tcagtgcaaa gattacagcg ttgtctccat tgcttagttc
     6601 tctttcaaca aatccaggac cagctaacaa agcaagtgcg acatatacga tcgacatcaa
     6661 gagcatgatc gatacagtag aatctcgcaa aaagctgaaa cttttaggaa tctttatgtc
     6721 ctctgtagaa cggtctttgt ttccaaaaaa ctttccgatc catccactag aagcataacc
     6781 gatatttccg gtatgcccta acgcaacttg attgtttcca gtgatgttct tcataaacgg
     6841 ttgacaaaga gcaggcgtaa tagttaatag tgttccttcg aagaccccgc ctaataagat
     6901 catccacaaa tttgcagtaa accccgcgct tactaaaatg actgctgtta gacaagaaac
     6961 ataaagcatt gcttgacctg taagaaaaat gaatttatat cttgtgaatc tagcaagtaa
     7021 aatattcacg atcatcccta ctagcatgat caaagcagta ggtgtaccgt attgttgcaa
     7081 agcaatcgcc actactgctt cattgctagg aactactcct tgagtatgca gagctgtttg
     7141 gaacatcgaa gcaaaagggg ttagtgaatt actgattaaa ccagcaccgc cgatcagtac
     7201 taggaatccg acaaatgttt tgatagttcc ttggatcact tctgaaaatt tcttcttctg
     7261 aagaacgaga ccaagcagtg caatcaaaga aacgaggatg gagggctgac tgacgattga
     7321 cacaaacaaa ttcaacaatt tatccattga tagttcctcc cttgaatcat ttttattctt
     7381 ttgacaagat cttcgttact ttttctttta cttcttcgct gtcaatgatc gattgcaaag
     7441 gtacgagctt ttcatcaggt atagaagtta gcgtatcttt caaagaatgc tcaatgaaga
     7501 aataatcagc catatcgcta gatacacttc caacatccgt atgacttact tcaaactcgt
     7561 ccacatctgc tccaagatct gcaagtgctt gtttgatgtt catctctacc ataaaacttg
     7621 ttcctaatcc actttgacat actgcaaaaa ttttcatatc attcgtctcc ttttcttatg
     7681 atcgctaata tttcttcttt tgttgatgcg tgtaataaat cttgaagatt ttgtttatta
     7741 gaaagtagct ttgttaaatt tgccaaagcc cgaagatgtg cctcattatc gatagcagct
     7801 aaacagatga acaaactaat gggatgggct ggattatcaa gcagatccac aggttgttga
     7861 agtttcagca aagacatccc tacttcgttt acgccatctt ctggacgagc atgaggtaat
     7921 gcgatgtgat cgccaatatg gataaacgca ccatactgct ccacgcgctc gatcatagct
     7981 ttgatataac tttcttctat tttattttct gataatagcg gctgtgcggc aaatgtgatt
     8041 gcttcttgcc agttttttgg ttgatccgat aattggatca tttcttctgt taataattca
     8101 gatagcatag gacgttgatc ctccttcttt tctaaaagtt tgttcatttt acgatttaat
     8161 acttgataga gcttttcttc gtctacgcct tcttttaatt cgatataagg cagtagcgcc
     8221 gacaacaatt cttgggcaga aggcattgaa aatcctggta tcagccaatc tctttgtact
     8281 tgattgatca gctgattttt ttccaagtca gtcatgattg gatgcagaac atagagagac
     8341 tttttcttat cagtaagtgt gagaggtaca gtagaaaaaa taatatcgta agaagtttcg
     8401 tcaacctcgt gtaagcggtc agctgaatta gtctctgtaa aaaagatcat cgggaataat
     8461 tgctgaagtt cactttttaa gatcaatgaa gagctaatgc cacttggaca gacgatcacg
     8521 gctcttattt ttctatttct ttcttctgcg tccgcttttc tgatctctcc gccaaatagt
     8581 atagtgaaaa aaccaatctc ttcttcaggg atgctcttcc ctattcgtct ttctaacgga
     8641 agtaatgctt ttcttgtcat ttcgtagatg gaagcatatt gatgcttaat ttgatcgatc
     8701 atcacattag gtaaataaaa agaataggaa atacgaaaat aggcagggac caagtgataa
     8761 aagacattca tcaataattc acgaaacgaa gcaaattcta cggccatcag gaattccatg
     8821 cgatggatga cttctgaaga acattccagc aaaaaatcaa gtgcaggctc ctggacatct
     8881 ccttcaacga tcgatgctaa gcaaagagag aaaaatataa tctccgaatt ttgatgttcg
     8941 gaatagccaa ggccagtcaa tacttgtcga ctaatgtcag tgactgcaag agaagccaac
     9001 agttcagcat cttttaccag ttccatggat gtttttccaa ttcgaatggt tgtcaaactc
     9061 aaataataag ctaattcatc cattcgattt ggtgcgaatc gaagatctga tagtgaaatc
     9121 gttttatcta gcaaatctct aactttggcg taataatcaa tggaaagggt tgcggttaaa
     9181 tacgtgagcc caaatcttcc agcaagtgac tgcatcaaac gagcagtgcg attttttgct
     9241 tctcttctta atattgcttc tgaccctagc agcctaaaac cagttttcct agtatacgac
     9301 agctgacaat cttccacttc taattcattt cttatctttt tgatatctga caggactgtc
     9361 cctttagaaa cctgaagaaa atcttgaaaa tgaaaaacag aaacagacgt tgcagtcgaa
     9421 taaaccatca aataaagaag atctcttcgc tcttgttcgg tatacacagc ttcaaatcga
     9481 gtaatatcca aaaatttttc tgcccagtcg attgctcgga atttctttat acggatacgg
     9541 gtatcatcca catattcggc aaaaggagtt aattgtttga tcgcctcgct tgtatttact
     9601 tcgatgatct ctgctgcttt ttctagactg atttctcttg gtgttttcag gagataacta
     9661 attttgtagt tcacagcgct gttcacctcc tacttatatt ctagtatggt agcgctttta
     9721 aatcgagggg tgtttggtac aacttacagt tggatttttt ttacgactgt accgaacgat
     9781 ttctatcgaa tggatacaga aaaaaagcag gtgaccaaat gtctcctgct tttatgaatt
     9841 gatttcgtcc gatcaatgat gatcatgttt attttttgag gtcagttctt caaatttttc
     9901 tttgacatct tcaaccgctt tttcgatatc tttttcaaag tgattgggct cattaggctt
     9961 tgtttcctct tctacagaat ttgaaccaga taaagctgtt tctttatttt ctgtttcacc
    10021 agagtttgtg ccagctaatg attcttcttt gtcttctgtt tcaccagaat tagttcctgc
    10081 taaagccact tcttcttctg ttttaggggc cattgaacct cttaatcgtt tgtgtttttc
    10141 accgtcataa tttgtcatgt tggttcctcc tagtagttct atactttttc atcgtacaat
    10201 aagacagttt atgcgtcaaa aaacaaaccc gattcttaca aatatgtcat atttttcctt
    10261 ctgattcttg aatgcttttg attccttctt ccaaaatttt catttgtaag attgtttctt
    10321 catctttgat gactttttct gcgccattaa ctaatgtctc gtaaatccct tcgtagacac
    10381 ggctgtaatc accaacctcg gaaattactt tttcttcatg ataactccct tgttcatcta
    10441 tataggtcaa tacaccataa tgttcgggca aatcgatacc aaagtcttca tgatcaggca
    10501 tgtaaaacat tttcagatgt tcttcttgtc gatcttttgt ttgtttcacg aacatccctt
    10561 tttttccgta aacgacgaaa cttggacgtt ctttgatgcg gaaataactg gattttatcg
    10621 aaattttcat atttccatag aaaaaatcca gatcgaagta atcattcatt cgtcctttcc
    10681 ctaaaagttg acgtacatca tagctgatat catctggttc gccgaaataa gaaagaactt
    10741 gatctactgt atgacaagca tgtccatata aatagctgtt cgcagtagaa aattcatgcg
    10801 tattttctgg tacttccggt ctgaaataat cataatgcat ttctacttct aaaatatccc
    10861 ctaattttcc gctttcgatt actttttgga ctgtcaagaa gtcagaatcg aaccgtctgt
    10921 tttggtaaca ttggataaat agaccttttt cttttgctaa tgcaaataac tcttctgctt
    10981 cctttgacgt ttctgcaaaa ggtttctcta ctagtacatt cttaccatgt tcgagtgcca
    11041 tttttccata ttcataatga aattgactag gtgtagtgat gacaactaat tggatctcag
    11101 gatcatccca aagatcagac agctgatcag tatattcgat gtcttcgaat gttttccaca
    11161 ctggttcaga tggggaaaaa atttttttga tattgatttt gttcgtaagt tttcttgaaa
    11221 aaggcaaatg atagcgattt gtactctttc cattgccaag ataagcgatt gtaagcatga
    11281 gtggtgcctc cttttattga tgttttcagt atatcctaga aaagcgcaga aagtatttat
    11341 aatagctatt ctggtaaaaa agttcagaaa aacacttgtt atcattcggt ttggtgaaat
    11401 taatgttcaa agtaaggata aaaatcaaaa agatagtagc ggaaccaagt gtttatcagt
    11461 aagatagaat ttggaggtca gatcatgctt attgaagaaa aactaacaaa acaagagttg
    11521 tttaccacga cagagaaacg gattgtcgat tacatccgta ggaatatcga agctgcagtc
    11581 tacatgacga ttgaagaatt agcaaaagct acgtatactt cccattcagc tatcattcgt
    11641 ttatgcaaaa aaatgggatt cagcggattt aaagaattcc gtttcgagct ggggcgggaa
    11701 gttcatcaat tgattgctca gttcaatcaa acgattgatc ctaattttcc tttttcaagt
    11761 gaagatagca ctcagcaaat tgcaaagaaa atggcggatt tatcgatcca gtcaataaaa
    11821 aaagcacaat tgcagattga acatgaagat ttagatcaaa tcggtaaagt attgaacaag
    11881 gcacagcgaa tctttctttt tgcaaaaggt gattctcaaa tcacggcaag gaaattccag
    11941 aataaaatgg tcaagttgaa taagtttttg atcatggctg aagaatacag tgattcttct
    12001 tggaatgcag ccaatttgac tagcgaggat tgcgccattt ttattagcta cagcggtcgc
    12061 atccatcatt atgaacgaat aatgacctat ttgaagcatg tagaggcccc taccttgctg
    12121 attaccggaa atcagcattc agaaatggcc aaacaggctg atatgtgtct tgttatatcg
    12181 caggaggaat atgattttgc taaagtagcg actttctcct ctcaaattgc cttcgattac
    12241 gtattaaata cgttattctc agtgatatat tctcaaaatt ttgaagagaa tatccgcaac
    12301 ttgaaaaaca aacaagaatt gatgcaaaaa ggccttctta aagaatatcg gaaaacttaa
    12361 gaaaagatcc tcatttagta ataagaaaga tagtgtttga taggctctct ttgatatcat
    12421 cataaaaaca ggctgaagtt ttctttttca aacctcagcc tgtttgttta cggaacgatt
    12481 cgaatagata tattatagaa atgaggaata gctaacttct gtttcttatt ttgaacgatt
    12541 gacgaaaaag atatataaga aggaggaaaa tcagtataca tgcagctaac aatccataca
    12601 ggaaaaatcg tttgttctcg gatactttct gtacttcttt ttgaacttct tttgcatgat
    12661 aaggaacccg gtgccctctg acaagtaagc gatgactgtt gaccatataa ggtgtacaag
    12721 ttaggagtgt cacgaaatcc tgcccttttt cgattctcag atcttttgta tcagtcggtt
    12781 ctaccgtctt gatctgatcg acttgataag cgagtgtccg tccattgatt tcgatataga
    12841 aagaatcacc taaagtcagt ttcggtaagt ctgtgaacaa ctttgcttga ggcagtcctc
    12901 gatggctgga aataacggca tgtgtatttt ctccccccgt aggataagac gttccttcca
    12961 ataatgaggc acctttttct agcagcacag tattcgtttc atcaaaaatt ggcaaatgga
    13021 cattgatctc agggatcgtc aaaataccga tcgtatgttt ctcaaaatag gttctatcct
    13081 ttttcgcagg ttcttttttc ttagtaaagg gatctgcacc tggatttcca ccttcttttg
    13141 ctaattgctg gtttttcttt gtcatgtttt cttggatttt agccatgact tcttcatttt
    13201 ctttgaccgc ttgttgctga taatgagaga tgatctgctg atccagataa ttattcagcg
    13261 cgtcgctaac aaatggataa gctagtatcc ctattcctga gagaagcaag aaaagcatca
    13321 gcagatctag aagtttcctt ttctttggtt tcatttgtgt tctccttgtg atgactgttt
    13381 gataataaaa gaaaaaggag catttgcgta atccgctgct cctttttcca attcttgcaa
    13441 aaagactaaa tctgactgtg cttgcgtcta gcaaagtaca gtccagcaat cagtagaagg
    13501 actactcctg caccgatata cacatagatt cccttaccgc ctgttgaagg aagtgtacct
    13561 ttgtgtttat ttggtatttt ttcaggagaa atcaactgtc ctgctgttac ataagattgt
    13621 tcatcgatag taaatgctac acggtttgtt aatggaacat atttttctgg cgctttcgtt
    13681 tcttccagat agtacgtgcc atattttaga cctgtcacat cgattaaacc gttacttgtg
    13741 gttgtaaaaa ccgttgctga ttctttcgcc gatacccagc tgacggcttt tgtggatggg
    13801 tcgatcgata aatatttcgc tgtgtcacta tcttgatcac gaacgacgaa ttctgctcca
    13861 gcaagtgttt ggtctgatgt aacgtcacca tctactttaa caaatcgttt gcccccagta
    13921 acgacatcga ctgacggtgg tgtttgatca tttgtatgcc cgttatcgac atttgcttgg
    13981 ttagaaaatc ctttggttgg atctgctttt tcgttcaaat gcatatagta aacgaatttc
    14041 aatgtaccgc caggagttaa tgaaggaata taattcggat caactgaaac cgtgaatccg
    14101 tctgtttgct cagtgacaga ataatttact gggtcgattt ctttatttcc atcatataag
    14161 gcataagcat acgttcctga agaatcatta tcaaatgtta aagcagcgtc atgagtatcg
    14221 ataagtttga atgttgtgta cttgttttgc gttccttctt tatcagcgat acctaatggg
    14281 atattgacag aaatctcata ttgaattttt tcaccgattg cgacatcttt tccattcaat
    14341 tgaggtgttg ttttatcaac tttagacgta tcatttttga tggtcttttc tactggtgtt
    14401 tggctatttg ccaggatctc aaaaggcgtg attgtttgct tttcaatcat ttccgcatta
    14461 tcaggagctt ttacttcttc taaattatat tttccaactt ctaaatgatt aacgatcaac
    14521 tggccttttt caatagatgc ctcggcaaag tcattgttgc cgatgtcata agaatgacca
    14581 gtaatgaaat gtttggcttt ggtttgatca gttgtccaag tgtacaatcc atctgtgaca
    14641 ctttggatgt attttttgac gcttggtgtt ccttcttctt tagaaataat aaattctgct
    14701 ccatttagtg cttcgttttc ggcagtaccg atttttgtaa ctttcaacgt tccatcatta
    14761 ccgactgtat ttttagggta gagatggata gtatctagtt cttctgtccc gtatttataa
    14821 gagccatctg cttgtttgat catctcatat acagggaaag ctaaaaccat gtttgcggca
    14881 gctgacactc cgtcttttgg ttcttctttg atcgtgtaga ctgcatcttt cccattttgt
    14941 tttttaggta aagataaagt gacatttcca tcagcatctg tcgttcctga agcaactggt
    15001 gtaccaggag tcaaagactg gactgcttgt tttgctgcat ccacggacgc tcctttatct
    15061 cgttgcgcat aaaattcttg agtgacgtta taaactgaaa atgaaatatc ggctaatcct
    15121 tggtattgat cgaattcgct catttctttc ccgctgtttt ggattaaagg atcgggtaaa
    15181 tcagtcattt tctttttatg aaggatgact tgagctgctg tctcctctgc aaacactttt
    15241 tttgcgccga agctgtttgt gagtaatggt aaaataagga aaaggactcc taacataacg
    15301 tttatttttt tatgattttt cattttttca ctcctgttct ttttttgata aacaaccaga
    15361 ttgcaaagct tgcgaatacc ataccaacaa cgcctaaaag tgtatttttc gtttcgtttg
    15421 tttttggaag aaatggacgg ctattttctt ttttattgac tactttcaat atactgtccg
    15481 cctctttatc ctcagaaaga aaagttgaaa tggtaaacgg aatttctgtt tcacttaaaa
    15541 tataaccaga aggtgcttcg atctctttta atcgataact tcctgttttc aacccggaaa
    15601 ttgaaaaagc gccattttta tcggaaatca ggtgaagcgc atctgattcg ttctttgtcc
    15661 acttgtatcc gttggctgtt tgctggaggt aatttcctgc tttgttttta ataaggaata
    15721 ctgcgccagc taatcgttta tctggtttat ttgtctcgat tttgacaaat tgcttactcc
    15781 ctgtatatac agagactttc tttgtactga caccaaaacc aaaatccagt gttccgttgt
    15841 tgttgatttc cttgttcgcc tgtgctgtac tgcttagctg catctgataa gaaatggtca
    15901 gtttttttcc agcatatttt tccaacgaag ggatcgaaaa taaaacatca aatccgtgag
    15961 gtttcttgtg tatgacaaag ccttctgtca gcttttctcc attcgatgaa atcgttaaac
    16021 tattaggtaa aaacgttaat gcttcatccg cactatctga caattcgaac ttttgatagt
    16081 caaggatatt tacaggaatt gtcgtatgca aggaataagt gattttttca ccaatcgtaa
    16141 aatcatttct aggctcttct aataccttct caaaaggtgg atcagggtag tcgttttctt
    16201 catttttagg atacaaatgg atagttgaaa gtttttgatt gttttgtcca tgtacaggaa
    16261 gaacaactac catattctct gccttttctt tgacgacttc tggtactttg gattcaatga
    16321 aaagatacgc tttatctctt ttttcttgat ctttagcggc cagagaaaaa gaagcgatac
    16381 catcttcgtt gttaagagtt gtagttgttg cttctgcggt aaacataccg gaagacgcac
    16441 cgttttttgc gatctctgct tgtgcttctt ctaccgtttt gcccttttcc cgtagatggt
    16501 aaaaagaatc tgtgacatca taaacttgga atgtgacacc atttaatcct cgatacgttt
    16561 gtaataaagc tttttcttgt ccgtcatttg gatgattttt cggcagttgc ccgtttggga
    16621 aaagcaattt gtgtaaagta atctgaacca tttctgtttc tgtttctgtt gctacctgag
    16681 taaaagctgg tttaaatagt aacaagaaga gacacatact aagccaacca agttttttca
    16741 tatttagctg ccccctcttt cttttttcaa aaaaagatac caaatagaaa acgcacaacc
    16801 aatcatccct actagataga ttcctaaacg gccaattcct cccgtttcag gtaatggtac
    16861 ttttgcctga ttcgtgatgt ctaaagaaat ctggttgttt ttggctcctg gtgacagaac
    16921 agattcatga tcttgtccat ccacttgaat tgacccatct tcgtgtataa ctatagtaac
    16981 tggctctttt agaccttggt atccttctgg tgtaaaagtt tcggtcagcg tataagttcc
    17041 aggttttaaa ttttcgaata gaaaggtatc tgtttcttgt ccatcttttg gcgattctaa
    17101 gtccatttct ggtccctgca gtctgaattt tgctcctttt aatgtctgtc cgttgtcttc
    17161 ttttttgtgt accgttagat caaacgcttt tagtcgattt tgatgagtga gcgtccatac
    17221 attatttgtc atctcggtat tttttcccgt gtaaatgatt tttccatatt tatcgatttg
    17281 gaattcataa tttcccggcg aagtatcata tccggcagga ccagctgttt caacgactcg
    17341 atatttacca ggtgtgagtg aatcaaaact ggcaagacct ttttcattcg ttgtttggct
    17401 gtcaattggc tgataagtac catttgcttc ttttttctgt agggaaaatg ctgctccttt
    17461 aagatttatc tctttgtccg tcccttgcat cgcatactta cgtactgcta caggaacttc
    17521 aacaaaagga ttttcaatag tcaactggat cgtatcatcg gaagtagtga ctgtttttcc
    17581 atcaatggtt accgtaccat cagaagcgat cttgatttcc caagtagtct ttttgcttaa
    17641 tccatgccct tctggagctt ttgtttctgt cagcgtatag gtcatttctt tttgcaattt
    17701 gacattttct ggaagagaat aggttccgtc gccatgatct gttagtaaag tatcaataga
    17761 atctcctgtt aatttgaaaa cagcgccaat aagatccttt tcacctgtag aggaattttt
    17821 cgttattttc aagtttaacg gtgtgaattg tttagtattt ttccatgtca ttgcatctat
    17881 ttgttgagaa tcgtatcctg gtacaggtaa ttctttgatt gtttggtaac tgaatgcttg
    17941 accttgatta ttgtattgag gtagtgataa tatctcttga taactttctc cgctatttgc
    18001 agataatttg tcaatgtctg cacgttccca cgtatttgtt gtatctttag ctggtttaat
    18061 gattcgaata tatccgtttt tccaagctgc agcatttgtc gtatgttccc gttgaatctc
    18121 aaaagtaact tgatctggac gatcagctag attgttgtca aattcttccc ataacttttt
    18181 gatgtgaaga ctgactccgg gagctttagc agatggtata ccgaattcag ctaattcatt
    18241 ggtatcaatg tttggctgga aagttgtcct gccgttcatt tgataccaga aatttggatg
    18301 gaagtcctca ttttctgttt ggattctcac ttggtaatgg atttggattt cttggtcttt
    18361 tcctaaatag atctgattgc tcttgatggt atttccttct atggaaatag atggagtgac
    18421 cgttgtagga cttgtcccca cactcttgac tgaaagtgta ccaggctgat aaatgaatgg
    18481 ttctgcgatt ggatcattta tttgtccatt gcttacagtt gctgagatct gtacagcttt
    18541 tttggctagg tattcagaga tatctgttgc atgatcagct gattcatagt aaagatcgcc
    18601 tttttcatct gatgaaacca tttgtcgcat acgagactct acttctgctt ttgagagacc
    18661 agctgccgga tcgctttgaa gttggatgcc aagaccatgt atttcgattc ctcgttcttt
    18721 gagtgccatc gcttctccga tggttgcgat aaacgtactg tcgattcttc tggatagatt
    18781 gttttggtca ggtgcatcat agattcttga gattagagaa gtatttcccg gccgatcttg
    18841 cgtattagaa aactgagttc cgtaataatt gctgcttgat tgtgcgtgta cccgctgtac
    18901 tttataggaa aatgttggta caccatccgt cagcaaaacg atcacttttt tatgtccatt
    18961 tggaacggat agcatgcgct tcctgcatct cttagtgctt tttgtgtaaa agtaccacca
    19021 tttgtccgtg aaggtgtaat ggattttact tgatttttca ctgaatcaaa tgaacccatc
    19081 tgtactgcac cgttactgta gctatatcct tcgcttgagt agccgacata tcccatattg
    19141 attttgtctg tgataccgct atctgctaaa gtatcgacaa aacgatcgac accaatcttt
    19201 acttcaccga tccgattatt gtcgttcata cttcctgacc agtctacgac caatacgaga
    19261 tcaagaggcg tgatatcttt ttgtacattt ccacgagcat tcaaataaac atcaaacaat
    19321 ccgggtgtac ttgtttcctt tgcatacttt cgtatcgcat agtctgcttg attaccggtt
    19381 cctccatatt cgatatacga atgggtccga tcattaggat ctccattcca agaatttata
    19441 ccatcccatt gatttgttcc gtttttattg ccttgatgat caagaacatt tgtatttcct
    19501 gtaggttgcc aactggcttt aggataagtg ccctgatcat ctgttgtata ttctgtagga
    19561 atcgtttgat tagatggcac actcacagct ggcaaggata atgtaggcaa tgtaaatggg
    19621 ctgacagaat tttcgttttc ttttgatgat tcttcttcta tttcgtctgg ctttggtact
    19681 gacacatctg tatctttctt attttctgtc tcatcaataa tgtcatattc tttattatcg
    19741 acgatcagct tgtttgcctg gctgtggtaa agtgctggat cgattggaaa actcagatcg
    19801 atagtttgtt tagtgtttga tgagatttcc aacgtaattt tcccgctatt tacctgataa
    19861 gcatagccat ctccttcttt tctttccaca tcacttaagt aattttcgac tagaccaatt
    19921 tctttttcta tcttttcagc actggtgtta ttcgcattta gcttgatgtt cagtgctttt
    19981 tttccagact gatccgaaat gacttcagct gaaattgttt ctaaaactgt tggatcatct
    20041 gtagtgtctg ctattgcaga taatggcaca aagtttgata aactcaatat tatcagcatg
    20101 aaaataactt tcagtttctt ccctgtggtc gtcatttttc ccctcctttt tctttttttc
    20161 tgttttgacg tgaatagtga aaattattaa aataaacagg cgttacataa ttttatttat
    20221 tttaatatga taattattat acgaagggcg ctaaagggaa tcacaccaat tttcatacca
    20281 attggtttca tttatgaagt tgattccttc gattaatgag cgtttcattg aaagaaaagg
    20341 gttgagggag aaaaacatat gatcaggttg ttggaacgaa actatagaaa gatggaaaaa
    20401 atcattcgaa ttctgtccat ttctgacaag tggatgacta aaaaagaact tgccaaacga
    20461 attggtagtt cagaatctac atttgtaagg tatatcgaag agatcaaaat ccgctgggga
    20521 acggatgtaa cgattcaatc ttcacaaaaa ttggggtatc gtcttgaaca tttgaatgta
    20581 ccagcttatt taaatgttct gatggaaatg tcgcaaagtt ctacaaacag tcgtttatta
    20641 aatgagatta ttgtcaatcc gggtaaaacg attgagtatt attgtgaaaa aatttccatc
    20701 agccggtctt cttttacacg taagttgaaa caatgcaatc aaattttaga aagctattta
    20761 ttaaaagtga tcgttgacca aggttttcag ttaactagcc ttaaaagtga gctccagttg
    20821 cgtatctttg ttaccttttt ctttttaagt tactacggaa gacgcacgtt gccatttcca
    20881 atcgaaaaaa agaagatcaa agaaattatg gtcaagaata attgtcagat gaatttgttt
    20941 caggaagaaa gcccatatga gcaatctttc tttattatgt attttgttgt gcatctcatg
    21001 agagaatccc aaggaactcc atttgaatgg aaagctggag actcttatgt acagttgata
    21061 aatatcgata aaaaagactt cttaatcttg aaaaatctct taggaaaatt gaagtacagc
    21121 agctatataa aagtagtgaa ctattttgtc aatacgattt tttcagaatt ttcggagaaa
    21181 gaaaaaaatg aaatcagtga gcagatacga aaaaatatgt cctactgttc gttttcatcc
    21241 gctgattgca aagaaaaaaa cgaagaatat gttgttcaat tattaacgca aatttatttt
    21301 ttctcccgta ttttcccata cgatacgact catttgacga tccgtgcaac ttactttgca
    21361 cagcagatgc aacaccgcca gatacgtcta tatgctggat ttcgtgcaga acttcaattt
    21421 tattccactc tactgaatca gaatctgatt tattatttat ctagctatat cttttggatg
    21481 cttatcggta atcctgaaat tcaccattat acttctgata aaaaaatact gattatcagc
    21541 gacttaagtc ttcgacatag tcaatatatt gaagaatata tcagtgatat tcttgctgtg
    21601 cataaaattc actctgagac aactgctatt actgaagatc agctgtcgaa atacaatctt
    21661 ctcgagtatg atctgatcgt tacgaatcaa cccattttga atgcccatgt tcctcatata
    21721 ttgattgatg atagtgtgtc attcgcaaat gaagaagaac tcatccgctt attcgagtta
    21781 tgaataatca aaaaacagct atgtataaac caccctgata aaaatttcag gacaaataca
    21841 catagctgtt tctttttatt gtattccttc agcaatccaa cgagatttat agcgaaagtc
    21901 ttttaacaac tcttcttttg tccaattatt tgcaatgaga tccgtcaaag tgttcattac
    21961 gagggtcatg gaaaagtata ggaaactaac tgcttcttcc gacgattgaa tggagaaatg
    22021 attgattctt aataattcca gccatttttc gttcattgga gaatcagccg atagttctct
    22081 acgtttggtt gtctgctgat catttcgctg ggttaacaag tataaccttt gccaatagat
    22141 actttcgtga tctaagtcgc agcaccactc taaatatttt tcgatcccca aaaagaaatg
    22201 ttgtttgttt tcgctaatga attttaagat atcttgatgg actatgtgag aactgctttt
    22261 gatcgtatac aaataagcat cttccaaatc ttgaaaatat ttataaaaag caccgcgcga
    22321 catccccatc ttgtcaacga tttctgagac tttgacttgg ctgatgtgct gtgagcaaaa
    22381 tatctccagt aataggtcat cgattctctg tttctttgcg ttcgataaat tgaaaaaagt
    22441 ttcttttggc atccacatct ctcctttatc tctgattata cgtggatagc ccttcattgt
    22501 catgaaggat tcattccttc tttttctctt cttctgtcaa gtacccatct tccattcgat
    22561 aaacgcgatc actccattga atcatacgga catcatgagt aaccataata gtcgcttttt
    22621 gtttctcatg agcttcttta actagtaatt tgacgacttc atatgcatga tctgtatcca
    22681 aactagctgt tggttcgtct gctaagatca agcttggctc attatataaa gcgcgagcaa
    22741 ttgccactct ctgtctttct ccaccagaaa ggtctttagg ataactattt ttaagtgact
    22801 ggatatctaa agattgcagc aaggaatcga tttttgttga atcttctttt tttgaagtaa
    22861 cttggtcaac tagttcaaat tgttccttta ccgttaaaaa aggaatcaaa ttagagcttt
    22921 ggagaataaa tcctatttct ttaaagcgta aatctgctcg ttttttttct ggaagctcag
    22981 ttagtgagtg accgttgatc actatttctc cggaagtcgg tgtttgcaat cctcctgaaa
    23041 tggtaaggaa agtactttta cctgaaccag aaggaccaat gatgctgacg aattctcctt
    23101 cctttacata aaaatcaatt gctttcaaag ctgagacctt tgtatgacca gaaccaaatg
    23161 ttttggtaac ttttttcatc gttaatattt ttttcatttt tattaacctc ctattgctgt
    23221 aattgggtct actttggtta tcgtacgtat cgaaaacaat ccgcctagca ttgcgacaat
    23281 gatcaaaatc ccactatata gcaaccattg tgaccaaaaa acagcaaaag gcatcgcgct
    23341 tggtaaaatc agactagtca aataagcaaa caaaagagca agcagaacgc ctaccacccc
    23401 aacaataaag gactgtgcta ctaaagattt agcgatgaag aaatttttga tcccttgtgc
    23461 tttcattact ccaaaaatag cagtcttctg aagtgtcatc acgtacatga aaataccgac
    23521 cacggcagca accactaaga acaaaaagta tatcatcgca tttaaggtca tattttgggc
    23581 agaatagcca ggaatacttt caatgaatgt cccaatagtt aataactgta gacccttcga
    23641 tgctttttct ttcgaaattt tagctgaatc ctttattacg atcccattga tcggctgttc
    23701 ttcttcagaa gcaaacggct gttgtccata cttcaaagca gtccacgtat ctagggacgc
    23761 atagatgacg ggagaaactg tgtaataagt ttcaggaaag ataccgacaa tcgtcaaagg
    23821 atcgtcgtaa ctgccaattt tgatcgtatc tccaatttta tagcctgaat cagccagatt
    23881 ttgagagatg atgatttcat tctttgtttt aaattcatgc cctttcgtga gttttggtaa
    23941 taaaaatgct tctttcgttg tgccgaaaac tgtaacattt tctttgtttt ttcctttgat
    24001 tgctccgctg tacaatccga ttggtgcttt ctctcctcct tcaatgtatt tcagatctcc
    24061 tcttgttaat tgtgaagctg caaatacttg atttgcatcc tccgacaaaa caatctcttg
    24121 tgcgtcccaa tcatcgattg cttttttaaa ctcttctgaa agtccattcg ctagtcctga
    24181 caacatgaat acgacatacg caatcaagaa cataataccg acgatcaagc catatcgtaa
    24241 tttagaatag cgaatttctt tgatagctag aaacatttgt ttttcctccc tatgatagat
    24301 tgatagtgac aaaatgtcac ttgttgaata gtgtaactct aaagtgacaa tttgtcactt
    24361 gtgttgtttt tttcttatca ataaattgtc taagacatct ttcgataaaa ccttaacatg
    24421 agtcataaaa aatttattta agccttattt atgctttcca tgataaacta aagtgaccaa
    24481 atcaatgagg tgggaaaatg attaaactat tagtagtaga agacgataaa gtattatcca
    24541 ataatataaa agaaatactc gctgcaatcg gcgagataac acaagtttat gatggtagtg
    24601 aaggactgtt cgagttggaa agtggagttt atgatcttgc aatcttggat ttgatgatgc
    24661 cggaaatgaa tggatatgaa gtgttagcaa aaatacggaa agaaaaaata cagactccag
    24721 tattgatttt gactgcaaaa gatagtttgg aagataagat tgaaggattt gaacgaggtg
    24781 cagatgatta tctaacaaaa cctttctatc gagaagaact gatcatgcgc gtgaaagcat
    24841 tattgaaacg ctcgctaggt ctctttgatg aaaatgttct gaagtataag gatatcagtt
    24901 gtgatttaaa aagcaatgaa gtcttgtata aagaacaagt tttgcccata caaggaaaag
    24961 agtttgaatt gctcgtttat tttttacaaa ataagggaat cattttgaca aaagagcaga
    25021 tatttgaccg gatctgggga tttgattctg atacgacgat tacagttatt gaagtttata
    25081 tgagcaactt acgaaaacat ctgcgaagta ctggattgga taaagaaatc aaaaccttac
    25141 gtaatgtcgg ttatatactg caaagggtgt gatgcaatga atggagatat tacacgaaag
    25201 caacgtagtc gcttttttgc gacaaatgta gcagcatttg cttttatctt ttttcttttg
    25261 ggattcatta ctttacagat attaaaccaa tctgcataca gagaaacaga tctgtcatta
    25321 gagaaaataa attttgattc tcgaacagta caattagaga tacaaagata tcagcaaggc
    25381 gatccctttt taaacccgac tcaaacggaa caaaacagaa caaacatgga acagccatta
    25441 atggaaccat caaatgaccg ttttaacact caagtgatct tgtggtctaa aaatggtgag
    25501 atattgaata aagcagctat tggtgggcgt ttcaaccaaa tcaaagaatt aaaaatggat
    25561 acgaaaaatc tgaataccat ccaaaatatt gaattaaatg aagacagtgg ctcatctgcc
    25621 ctctctttcc gttcgattac gaaaaaagcg gagacgaatc aagatggcgt tgcctacatc
    25681 caaattttag aaaatacaaa tcaaatcaag aattcattag cgacttttcg cacgattatg
    25741 attttgtgta tgattgtttt ttggattctc tctattggta tcagttatta cttgtctagt
    25801 ctaagcatgc gccctattat tctttcttgg agaagacaaa aagaatttgt tgagaatgct
    25861 tctcatgaat tgcgcacacc gttaacgatt attcaaaata gtttggaaca tctttttacc
    25921 aagcctaatc atactattat tgaagaatcc gaaagcatcg ctcaagcatt gtcagagacg
    25981 aggcgcctca ctggattgac ttcggatttg ctgacaatcg ctcggaatga ttctgatcag
    26041 cagctgcttt caaagcaaat gatcaataca caagaatata tcgaacaact tgttaaacca
    26101 tttcaagaga tggccatcat agatggaaaa gagtttattc tccataacga tgcaacatta
    26161 gaagtaccaa ttgatgaaaa aaaaatccat caagttttgg ttattttgct ggataatgca
    26221 ttaaaatata cgaatccaga agataagatc attttggatt catctgttaa ccataaagaa
    26281 tggctgattc acgtaaagaa tactggccct tctatcagtg aagaagatca agcaaggatt
    26341 tttgatcgat tttatcggga agatcaatct cgggcgaaag agactggcgg atacgggctt
    26401 ggcttggcaa ttgcaaaaca ggtaatcgaa cagcatcatg gacagatttc tgtatccaat
    26461 tatcttccac aaggagtcga ttttaaagtc caattgccgt tgaagcctaa aaaataatgc
    26521 ttatccctaa aaaagggctg tgacaaaaat cttgtcacag cccttttttg ttgcgaataa
    26581 acggtgttcc agaagtagct tcttcggaaa taagccgaaa tttctttaag ctgaccactt
    26641 ctgtcacaac ctctttagct tacgcttacg attttttatt gcggtcttca atgtgtacat
    26701 caccgcttgt gtcaatatcc aattcttctc ggctaacatc ttctgtcact ttttcaacat
    26761 cttcatgttc ttctttatga atcccaactt cttcacgaat aactgtatgt ttgcttacat
    26821 ctacttgctc ttctttgatt gggatagtaa tcgtgtcatc ttccaatttt tcgtctgttc
    26881 cctgtgaaga ttgatctgtt actggtttgc gttcgatgac gatttcttca cgtttgaccg
    26941 gtacttctac cgtttcagta tcgttgacca catgtttgtg gatgtcgact tctccagttg
    27001 tcacatcatg tgtatttaca tctaattttt cttcttttag ttgaattgta tcatcatcta
    27061 gatttggttt gcgatcgatg tcttcagtct tgcttgtacc cattgtatca gtctcgccag
    27121 gtacgtccat cggatcagtt cctggtgtca tattcaatgg atccgttgta ttttgaaagc
    27181 ctgtcgtgcc acctacgtcc attggatctg ttccagtgcc agtttctaat ccagtcccca
    27241 ttgtgcttgc tgggctaaca gtatcttgtg tatccattgt atcttctgtt gtttctggct
    27301 gacgatagcc ttttactgtg atgacaaatt ttccatcaga aatatctcct ctatattgat
    27361 ataaaggatc attttctggt gtagccgtct cagaatcata ggtatcaaaa gaaaaagtat
    27421 cttttatttt ttcccataaa gaacggtctt cttcaccatc catccgattt tcatctgttt
    27481 caacatctat accagacaat ccgtcaaatc ctagtctaga tgctgcttca tcagtagtat
    27541 aaataacgat atcatctcgt tcataccctt cgttacgcaa ccgttcaatt actgataatg
    27601 cttcttccct cgtactatag cttccgatga ccgttaaatt tttctcttcc atgtaaattc
    27661 ctcctagcaa atagatttat gattggtata ttgttactta taaatttatt ctataggaga
    27721 cgattgtttt ctacaaatat ttagattgga atttgattaa aacaatttaa ttgaaatttg
    27781 aaaacattta tgttaat
//
LOCUS       DACEKH010000028        27215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEKH010000028 DACEKH010000000
VERSION     DACEKH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.028"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..3123
                     /locus_tag="IW662_001134"
     CDS             82..3123
                     /locus_tag="IW662_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0865971.1"
                     /translation="MIHLVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDC
                     QKLLGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRF
                     KVAYFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKN
                     QYTGQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPF
                     NQGSNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDRE
                     VTKKTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIH
                     DQLNYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGR
                     KIIITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEY
                     YELEGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFE
                     PFHVYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLH
                     PWAVTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDL
                     DVLVAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGF
                     DEPLLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQ
                     TNLREEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKP
                     IMDRYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKE
                     TRENIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEII
                     HKINERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDK
                     VMEQYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3313..3897
                     /locus_tag="IW662_001135"
     CDS             3313..3897
                     /locus_tag="IW662_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAQ0865972.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFISKKAI"
     gene            3894..5033
                     /locus_tag="IW662_001136"
     CDS             3894..5033
                     /locus_tag="IW662_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0865973.1"
                     /translation="MNELFRVHLEKAVNTLLKTELTAFLDYEKYDRIGFNTGNSRNGS
                     YDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLFRKGITMSEIAD
                     LIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKE
                     AIHIVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAG
                     AVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFA
                     EKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQ
                     NEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            5411..7453
                     /locus_tag="IW662_001137"
     CDS             5411..7453
                     /locus_tag="IW662_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0865974.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            7464..8084
                     /locus_tag="IW662_001138"
     CDS             7464..8084
                     /locus_tag="IW662_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0865975.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            8096..8554
                     /locus_tag="IW662_001139"
     CDS             8096..8554
                     /locus_tag="IW662_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0865976.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            8573..8851
                     /locus_tag="IW662_001140"
     CDS             8573..8851
                     /locus_tag="IW662_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0865977.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            8876..10243
                     /locus_tag="IW662_001141"
     CDS             8876..10243
                     /locus_tag="IW662_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0865978.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            10258..11403
                     /gene="dgoD"
                     /locus_tag="IW662_001142"
     CDS             10258..11403
                     /gene="dgoD"
                     /locus_tag="IW662_001142"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAQ0865979.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            11430..11921
                     /locus_tag="IW662_001143"
     CDS             11430..11921
                     /locus_tag="IW662_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865980.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            12364..13563
                     /locus_tag="IW662_001144"
     CDS             12364..13563
                     /locus_tag="IW662_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0865981.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(14058..14816)
                     /locus_tag="IW662_001145"
     CDS             complement(14058..14816)
                     /locus_tag="IW662_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0865982.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            14984..15919
                     /gene="pfkB"
                     /locus_tag="IW662_001146"
     CDS             14984..15919
                     /gene="pfkB"
                     /locus_tag="IW662_001146"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0865983.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            15916..17361
                     /locus_tag="IW662_001147"
     CDS             15916..17361
                     /locus_tag="IW662_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0865984.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            17389..17844
                     /locus_tag="IW662_001148"
     CDS             17389..17844
                     /locus_tag="IW662_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0865985.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            17863..18837
                     /gene="lacD"
                     /locus_tag="IW662_001149"
     CDS             17863..18837
                     /gene="lacD"
                     /locus_tag="IW662_001149"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0865986.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(18948..19509)
                     /locus_tag="IW662_001150"
                     /pseudo
     CDS             complement(18948..19509)
                     /locus_tag="IW662_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            19653..20267
                     /locus_tag="IW662_001151"
     CDS             19653..20267
                     /locus_tag="IW662_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0865987.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            20508..20891
                     /locus_tag="IW662_001152"
     CDS             20508..20891
                     /locus_tag="IW662_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0865988.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            21254..21763
                     /locus_tag="IW662_001153"
     CDS             21254..21763
                     /locus_tag="IW662_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ0865989.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            21863..22513
                     /locus_tag="IW662_001154"
     CDS             21863..22513
                     /locus_tag="IW662_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0865990.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            22964..23902
                     /locus_tag="IW662_001155"
     CDS             22964..23902
                     /locus_tag="IW662_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0865991.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            23915..24967
                     /locus_tag="IW662_001156"
     CDS             23915..24967
                     /locus_tag="IW662_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0865992.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            25233..25868
                     /locus_tag="IW662_001157"
     CDS             25233..25868
                     /locus_tag="IW662_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0865993.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            26059..26850
                     /gene="msrA"
                     /locus_tag="IW662_001158"
     CDS             26059..26850
                     /gene="msrA"
                     /locus_tag="IW662_001158"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0865994.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         8740 a         4392 c         5747 g         8336 t
ORIGIN      
        1 atagctgaat tgatggaact tgagagttat aaaaaagctc tcatctacga atacgtaacc
       61 ggtaaaaaag aagcctagga ggtgattcat ttggtagaga acaaagaaat tcgttttgag
      121 caggatattg agacttcact gactacgtat ggcaaatgga agaaagaaac attcaatgaa
      181 tcaaactttg atcccataat tggtttggat tgtcaaaaat tgctaggctt tgtccagaag
      241 actcaaccaa aacagtgggc acgtcaccaa cgaaactata acaatgatgg agaagtcaag
      301 ttccttcaac gctttaatca agaggtagaa acccacggca tgttacatgt attgcgcaat
      361 gggatcaaag accgcggtgt gcgctttaaa gtagcttact tcaaacctgc ttcagagctg
      421 aatgatgaaa cgatggaaaa gtatgaagca aatagtttta cctgtactcg ccagtttgct
      481 tacagtccta ataaccataa aaccattgat atggttttgt cgttaaatgg tattccgctc
      541 gttgctttag aattgaaaaa ccagtacact ggtcaaacag tagaaaatgc caaactccag
      601 tttcaaaaag atcgtgatcc aaaagaattg gtatttcgtt ttaatcgccg cttcttagtc
      661 tattttgcgg tggaccatac agatgtttat atgacgacaa agttggacgg taaaaaaacg
      721 tacttcttgc cattcaatca aggttccaat gggccgggaa gagttggtgg agcaggcaat
      781 ccgtccaata aagatggcta cccaactgcc tacttgtggg aaaaggtgct tactagagac
      841 aatttgatgg atattatcca acgttttatg aatctagaga ttaaaaaaga aaagcaaggc
      901 gacagagaag tcacaaagaa aacgttgatt tttcctcgtt atcatcagtt agatgtggtt
      961 cgaaaattgg ttgcagatgt aaaaagccat ggtagtggag aaaattattt gatccagcat
     1021 agtgctggtt ctggaaaatc taatagtatt gcatggctgg cataccattt agcctctatt
     1081 catgatcaac tgaactaccc aattttttca tcaattatta ttgtgactga tcgaacagtg
     1141 ttggatcgcc agttgcaaaa tactatcatg aactttgatc atcagacagg tttggtagaa
     1201 acgattgacg atcgcaaatc ctctcaagat ttaaaagaag ccattaatga tgggcgtaag
     1261 atcattatta ccactttgca aaagttccca gtgatctatc aggaagtaga tcaagtggaa
     1321 ggtcgtaatt ttgctgtgat tgtggatgag gcacattctt cacaaactgg gaacagtgcc
     1381 cagaagctaa aagccgctct ttcggacaaa tctaaatcct tggaagagta ttatgaactg
     1441 gaaggcgaag ttgacgatgt attaccagat gctgaagatg aactagttca acaattggta
     1501 gcacaaggta aacatcaaaa tttaagtttc tttgctttta ccgctacacc gaaagaaaag
     1561 acattggaaa tgtttggtac gaaacaacca gatgggcatt tcgaaccctt tcatgtttac
     1621 tcgatgcgcc aggcgattga ggaaggcttt atcttagatg tgctgaagaa ctatatgacc
     1681 tatagtactt cctacaaaat tgcccgtgaa attgaagata acccagaatt accaaagagt
     1741 gaagcaaccc gagcgattgt tcgtttccaa tcattgcatc cttgggcagt tactcaaaag
     1801 acggctgtga tggttgagca attccgagcg gttactcgta cagctattgg tgggcaggca
     1861 aaagcaatgg tcgttaccgc atcacgctta cacgctgttc gctatatgaa agaatttaaa
     1921 aaatatatag cgaacaaagg ttataatgac ttggatgtgt tggttgcttt ttctggttcg
     1981 gtagtagatg gagagactga atatacagaa acgaagatga atgtcacaaa atctggtgag
     2041 aatgttcggg agaatcaatt aaaggaagct ttccatacag ataattttaa tatcttgatt
     2101 gttgctgaaa aatatcaaac gggttttgat gaaccgttgc tgcataccat gtttgtcgat
     2161 aagaagttaa agggtgttaa agctgttcag actttatcac gactcaatcg aacaatgcct
     2221 gggaagaagg atacctttat tcttgatttt gtaaatgatg ttgaagatat tgaagctgct
     2281 ttccaaccct tttatgaaca aacaaatttg agagaagaaa tcaatgttaa cttgatttat
     2341 gatactcaaa gcaagctacg aaaatttaat gtttacaatc aaggtgatat tgaacgtgtc
     2401 atgaaattgg tgaaacaagc ccaaaagcaa cagaatgagc gcttgcttgg acgaatttct
     2461 agcatgttta agccgattat ggatcgctat gaggaaatga acaaggataa tcagtaccag
     2521 tttcgggtga ctctacgtaa ctttggcaag tggtatgatt atatttcaca acttgatcgg
     2581 ttattcgata tggaactatt ggaggaacga atttttactg gatacttgct gaagtttcta
     2641 cctaaagaaa cacgagaaaa tattgatatt tctgacaaag taaagttgga atattataag
     2701 ttacgtcagg acttttccgg tgaaatccat ctagtgaata aagaaggcga gctagtaaat
     2761 ccaaagtcaa ttgatgcagg aatcaaaccg ccggatgaac gagattcact agaagaaatc
     2821 attcataaaa tcaatgaacg atttccaaat gattttgacg aaggagaccg ggtgttagta
     2881 gagattttgt atcggtcatt tgccgataac ccagatttga aggttgtcag catggctaag
     2941 aataacgatg ctcagatgtt tgaaaaatcg ctgttccctg atgtatttaa agataaggtg
     3001 atggagcagt acgagaagaa caatgcagct tttgagaaaa tgtttgggca tgatgataag
     3061 tactttgatc tagtatatac attagttgct agagatttat acaagttatt acggacgaaa
     3121 taaaatttat aaataaggat tagacaatac aatttctctc aaaaataaag gagaagttgt
     3181 attgtttgct tttaagaaag ccctgaaaaa tttgttacgt actaaattgt ctgaactctg
     3241 tcatatcagc tatgtcagaa taaaggaaat agatttattt ttataaacag tgtcgtctcg
     3301 gagatttaaa ctatgtcaac gacaattgaa gtgaacaaac aaagcgtgaa gcagtttctt
     3361 gaaacaggga aaatcaaaaa attcgttatt ccagaatatc aacgtcctta tgcctggact
     3421 gacgagcaaa accaagtact atttgatgat ttagcggagt acacagcaaa caataacgag
     3481 agtacttact ttttaggaag cattgtggct tatgaaaatg atcataacga gcaagaaatc
     3541 attgatggac agcaaaggat aactacgcta tttttatttt tgagagctat ctatgctaaa
     3601 ttagaaaata gttgtgaaaa ggaagcttta ttcttgaaaa gccaaattga acccgcgtta
     3661 tgggaacaag atgatttaac aggtgaggtt aaaccagata aaattctgat tatgtctcga
     3721 gtaatggggg atgaggggaa tgaagaattt gctagtattc tagtaagtgg tgaagcagat
     3781 gttaaatcaa aaagtaatta ttcgaagaac tatattttaa ttcaacatct attaaatgag
     3841 tatgcaacaa acgaaccact atcattttat cgttttatca gtaaaaaggc gatttgaatg
     3901 aattattccg tgtccatttg gaaaaagctg tcaatacgct tctcaaaacg gagttaacgg
     3961 ctttcctcga ttacgaaaag tacgatcgca ttggttttaa cacgggtaat tctcgtaacg
     4021 gctcctatga ccgtacggtc aagaccgagt acggggaact tcatctccag attccgcgcg
     4081 accgcaacgg cgagttcaag caacagactg ttcctgctta tagacggacg aatgatacct
     4141 tagaggagac cgtcattcac ctcttccgaa aaggtattac catgtcggaa atcgcagact
     4201 tgattgagaa aatgtatggg catcactaca cgccccaaac catgtccaat atgactaaat
     4261 catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt tatgccgcta
     4321 tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag gaagccattc
     4381 atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat gcgattgcac
     4441 caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa cgtggcgtga
     4501 aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc gtgcagcggt
     4561 tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat atcagtcaca
     4621 aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg tatcaagcgt
     4681 catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg aaaaccagtt
     4741 atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc tatgatttcc
     4801 cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt aataagcaaa
     4861 tcaagaaata cagccaccgc aaggaacagt tccaaaatga agagtcgatg gaacgtttct
     4921 tagtctcgtc ttttgatact tacaaccaaa aattcctagg tcgcagtcat aaaggtttcc
     4981 aacaggccga aggcgagctc gaacaaatgc tgagtcaacc gatggagaat tagaaacaat
     5041 ctacagtagg gaagaaccat ttacacaaaa ttattgacgc tcccaaaagc attgttttta
     5101 gcagtttaat attattcttt ttcaattaga ctgacttgat ttagttacgg aatagatact
     5161 ttaatgtgtt gaagaagcaa cgtcccgtgt ttatttacta aagcatccaa tattctaccg
     5221 aatatttaat attcacatta gccctaaaat aattgtcata ccatgttaca tttgatataa
     5281 cagcgttcat tttaaaaata gctgttagtg tgacaaaaca ttaaaccaag tgtgacaaaa
     5341 aacgtgatga aagctttccg gaaacgtatg tgaacaagta tataatgatt ttgtcaggag
     5401 gatagtcaaa atgaataaac gaattcgtga aatactcaaa ctgattatta aaaaccctga
     5461 aatgaaactt tccactttaa caagtgaact tgatttgaca cgtcgtcaaa tcaattacgc
     5521 gatcaatcaa tttaatgaag aattgaaaac aaagaacatt ccgttaattc agagaagtca
     5581 ttctggaaat attacggtac caatcgaggt tatccaaatg atatcacagc ctgattacga
     5641 cataggtgat gatcaggaac ttgtcgttct cactgatagt gaaagacaag cattgatagt
     5701 gatgactctg atcgctaata tcgattacat cagtctggat catttggtag atgcggttga
     5761 tgttagtaag acgactgtta tggaagatat caaacgaacg gaaccgttca tcagaaatta
     5821 tagtctgaca attcgatata atcggatgaa tggttaccaa ttggttggtt cggaacaccg
     5881 tatcttacag ctgttatcgg atctagtaaa acggtttccg atttttcgcc gagaaatgat
     5941 tcgcagaaaa ttggcacaag atgtttctga agaagaaatc gtccatttga tccataacat
     6001 ggaacaaatg ttgcatctta gttattctga tgagtcggta gactatttgc agacatcagc
     6061 gagattttta gtaagccgtg ggatcaagat gtcagataag cacgcgtttt ttcccgatga
     6121 tgtgacggat actcctgaat tccgaatgct gatgatcttg gtacatgaaa caggttggag
     6181 tctatccaaa agttatatgg agtggttcac gttattattt ttaacttcca atatttttga
     6241 gagcaaaact actcaaaatt atgattcgga tcaagaattg cgaaaattga tcagccaaat
     6301 ggttgaaaat ttccaaaatc aaacgttgat tttgatcgat gaccgagaag actttgaacg
     6361 taggatactg agtcatctgc gtccagcctg ttttcgaata cgctataact tgagtttagg
     6421 agtttattca atagatagtc tgatcaagga tagtaatcat gcgattttga ttgatttgat
     6481 gaaagaattg attattccta ttgagaattg gctaaataag gcctttccaa atgatgaatt
     6541 agatctttta tcctattact ttggctatca attaagtaat cataataaat tgaataagca
     6601 acgacaaagg gctgtagttg tttgtactaa tggtgtcatg gtctcaaagt tagtacgagc
     6661 caatatggaa aaactttttc cagagatcca ttttttagct tccctatctg tgcgtgattt
     6721 ctacaaattt gaagctgatt atgatctggt ctttactacg acgccactaa aaagttctat
     6781 gctgcagttt atgatcgagc caatcatgac atatcagcaa caaatcagtt tacgatatcg
     6841 agttttgagt gatttaggaa ttactaaggt tgatcgagca atggatgaat tgttaacaat
     6901 cattaggaaa tatgcaaata tcaccaatcc agtttcgttg cgagaagaac tgcagtactt
     6961 tttgatcaag gaagatggaa gcacgccgct ggacaacttc aatgtattgc cttcattgac
     7021 ttattacttg cggccgagtt atgtacaaat cgttgaagaa gagatgtctt gggaacgagc
     7081 agtggaggtg gcttgccgac cgttattgga acatcaaatc attgactcgc gttttatcgc
     7141 tgattgcttg gatcaaatca gacaaccggg atatgcaggt tacttgggaa tgaagacttg
     7201 cattccccat acaacggtcg ataaaggtgt aatcagtgac ggtgtcagca ttttagtttg
     7261 taaaaagccg atacgatttc ctaatggtga agatgtcagc ttgattttgc cgctttcatt
     7321 ttttgattta acgaagcatc tgcgagccat caatcaaatc gctgatatcg ccaaagatga
     7381 ttcattgatt gagcagttat tgattgctga tgacgaaaag acagtttatc aactattaag
     7441 acaaagatct taaggaggaa aacatgagac tagcagatta tcctaaattg acagtgatca
     7501 tgcgtggtta tacttttgat caggcagatg ctattttaca agcaatgcag gaatttgacc
     7561 atcaatttgc cgtagagatg acaatgaaca caaaaggagc tcttgaaaat atacatgaac
     7621 taaatcaacg ttatggtgac acgacttata tcggcgctgg gacggtaagg acattagaac
     7681 aagcccaagc ctgctatgag gctggtgcaa aatttttatt aggaccacat atgttcacaa
     7741 aagaaatgtt gtcttacgcc aataagaaag gattattggc ggtacctgcc gcaatgactc
     7801 cttcagaagt cgaccgtatg tttaaagaag gagctgatat tgtaaaagta tttcctgcag
     7861 cggttgttac tccacgtttt ttcaaggata ttcaagcacc tttaggtaag ttaccactaa
     7921 tgggggttgg tggaatcagt aaggagaatg cgaaagagtt ttttgaacaa ggtgctagct
     7981 atttaggtct tggttccggt atgttcaaca agcaagacat cgaagagcta aatgttaaaa
     8041 acttagcacg ttcgatgaaa gaattaattg aggctattaa ataggagtga ggactttgga
     8101 actttatttg aatgaggaac tcgtttttcg aaatgttcaa gcaaaaacag atagtgaagt
     8161 cttagcgttt ttagcaagcg agatgtacaa aaaaaactac gtgaaggagg aatatattca
     8221 agcaatccaa gaacgggaaa aagaataccc tactggatta ccatcaacac caccaggaat
     8281 cgcaattccc catgcaaact atgaaatggt caataaaaca actttagcaa ttgcgactct
     8341 taaagatccg gtcctatttc ataatatgga agataacaat gagcagttac caatccaaat
     8401 tgtgatcatg atggcgattg gggaaccaca tgggcaagtt gaaatgttac aaaaaattgt
     8461 tggaatcata caagatgagc cactgcggca agaaatgatc agagcaggca atgatactga
     8521 attattagaa ctattaaaga aagcggtttt ttaatataag gaggaaatca taatgaaacg
     8581 aattttagta gcatgcggaa atggtatcgc cacttcaaca gtagtagcaa caaaagttag
     8641 agagtatttg caacaacatg gcattgatgt ttcaacgact caaacaaaat tgatggaagt
     8701 tccaggaaaa gttcaagact atgatctatt ggtgacgact ggtcaatttg atggtcaaac
     8761 aggtggtgta ccagtaatca aagggatgcc gattttaaca ggtatcggtg ccgatcagac
     8821 aatggaagag attttgaact tactgaaata atagctgatc aaaggagctg aaaaaatgaa
     8881 aatcattatg aacgtcttta acttctttat tgatgctgga ccaacagtta tgttgccagt
     8941 catcatcacg attattggtt tgatttttgg gttaaagatt tctcgtgctt ttaaatcagg
     9001 tttgacatta ggtatcggtt ttgccgggat caagttgata ttggacttta tgacgaccaa
     9061 tgttggacca gctgccaagg cgatggttga tcgaactggt gttaaactag atgctttgga
     9121 tgttggctgg ggttcgattg cggctgttac ttgggcatcg ccaatcattc caatcttgat
     9181 ttttgccatt ctattggtca atatcgtttt gctgattttg aaacgtacac atacgctgga
     9241 tgttgatatc tggaactatc accatatggc aatcgttggt gttatagtgt attttgtgac
     9301 taagaatgtc ttcttaggtg taggtgcatc agtagtgatg gcaattgcga cttttaagat
     9361 ttcagactgg tcgcaaccga tggtagaaag tttcttcgga atccctggtg tttctttacc
     9421 aactgtttct gcgctttcat cactggttat tgcttggccg ttaaattggc tgctggatcg
     9481 tatcccgtta ttcagaaaat caaaattcac catcaaagat gcacaaaaat atttgggatt
     9541 ttttggtgat tctatgatca tggggttagt tattgggatg gtgattggtg ttttagccgg
     9601 ctatgatatc aaagcggttt tacaattagg tgtcagcatg tcagctgttt tggttttgat
     9661 tcctaagatg acttcattat ttatggaagg attgatgcca atttcagatg ctgcacaaaa
     9721 atggtcacaa aaaaaattca aaggtacacg tttatttatc ggattggatg ctgcagttgt
     9781 tgtaggtaat ccagatgtta tcacaacagc attgatcatt attccactaa cgattgcatt
     9841 agctttagta ttgccaggaa accgtgtgtt accattcgca gatttagcgg ttgttccttt
     9901 ccgcgtggca atggtcgttg ccttgactcg tggtaattta ttaaaaaata tcattattgg
     9961 cttagttgtg acagcttcat tgctatggtg tgggtcagct actgcgccaa tgctgacagc
    10021 aattgccaaa tcggttggta ttaacttagg aacttcttca atgttaatct catcatttgc
    10081 tgcgacttcc atgatccaaa gttatttggt atttatcgca tttgcttaca aaccaatcat
    10141 cggtattcca gtgttgttat tagcctttgc gggcgcatgg tattacttcg atggtatcaa
    10201 aaaagttaat catgaaatgg atgaaccatt gaaggcacaa tagtaggagg gtttaaattg
    10261 aaaatttcag atgtcacagt gtataaagta aaaccaagat ggatttttgt taagatctcg
    10321 actgacgaag gaatcgaagg ctggggagaa atgatctcag gaactaaaac agaaacggtt
    10381 gttgctggag cttatgagat aggaaaccgg ctgattggac gtaatccgtt tgaaatcgag
    10441 cgcctcttcc aagaaatgca tcgatctttc ttccgaggcg gtcctattaa tggaaccatc
    10501 gtttcgggtt tagaaatggc tctgtgggat atcaaaggaa aagcgtttaa tgttccggtt
    10561 tatgaattat tgggcggtgc agcacgtgat aaaatcaagg tttattcttg gattggtgga
    10621 gatcgaccaa gtgaagttgc agaacaagca caagatcggt ttgatcgggg ctttacggcc
    10681 gtcaagatga acgcgacaga agagctgcat tatatcgatt cctaccaaaa agttgatgaa
    10741 gtggtggaac gagttgcgtc tattcgcgaa cgttttggtg atgcaatgga tattggtgtt
    10801 gattttcatg gacgggtcca taaaccgatg gcaaaagtgc tagcaaaagc tttggagcca
    10861 ttccatccaa tgtttttgga agaagtcgtt ttacctgaaa atgaagaaca ctttaaagaa
    10921 gtagctgatt ctgtggctgt accattagct actggtgaac gcttgtacac tcgttggcaa
    10981 ttcaagaacc tctttaaaca aggtactgtt gatattatcc aaccagatgt ggctctttgt
    11041 gggggaatct tggaaacccg taaaatcgca gcgatggcgg aagcttacga catggctgtt
    11101 gcgcctcatg cgccatatgg tcctgttgca ctagcagcaa ctctgcaagt agactcatgt
    11161 acgccaaacg tctttatcca agaacaaagc ttagggatcc actataacaa aggttttgat
    11221 ctgttagatt ttgtgaagaa caaagaagtt ttccaatata aagacggcta cgtagatcta
    11281 ccttcaaaac caggtctggg cttggaaatg gatgaagaca aaatcaaaga aattgcacaa
    11341 gaaggtttga tttggaccaa tccgcaatgg aaaaattatg acggtacgat cgctgagtgg
    11401 tgataaatga aaataaagga tgatgaaaga tggcagaagc acttatgatc acagtttttt
    11461 taggactgct gattattttg caaataatcg atcaaaagaa aattatcgtg tttgctcaga
    11521 gtagtcctgt aaggaagagt ttgagtattt tactgacagt cgccatcctg ttgattttct
    11581 ggccggaaca tctgactgat cagattaaac tggttgcttt tgcttctgtc attttgatca
    11641 atggtttttt gaaggagggt ctagcaaaag attctgttgt tagatttgga gtattgaacg
    11701 gtaactttaa tcgatatcct caagtccaag tagaaaaact aggtgacgat aaatcattta
    11761 tttcatttta caaaagcaag aataattctt tttcaatgat ttttgataaa gatcaaactg
    11821 aaatgatgga tgtctttcaa gaacttgata tccattccaa aattacttta gatcaaagtt
    11881 cggaagaaaa aagagcagtt gtaaaaaaag ataatgaatg attctactac cgaaattatg
    11941 atttctttat cacgaataat gaaacttgtt ttaatgaatc atattgattg aagtatacaa
    12001 tgtatattaa gttttggaaa gaaatatatt ggtacaagat atagcaggaa tcgattactc
    12061 gatccctgct ttttttaact tcctttgcag caaaaaaagc tcgtaatcta agagatgatg
    12121 gatagttttt agaatatata tattttcttc aattccagaa aaaaatgacc aagctagtat
    12181 gctaaaaaag tacgatgctt ctatgatctt ttttccgtct ttattaacag atgttttaat
    12241 tgatcaacgc atattgtaat ctaccctaat ttccatagtt cttcaccact tttaaaaata
    12301 gtaaaacccc tacaagtatg ctatagtaaa ttgctaaaga aaaaccaata aggtgggaga
    12361 tgtgtgcgcg atcaaacaag cggaaaaatg attcgttttt attatacaag tttgcatttt
    12421 ctttattgga tgatgttttg ttctgtctat gggtttgcga cattgttttt gatttatttc
    12481 aagattgatc cagctaaaat cggagggatc ttagcattgg tcaatattgt ttcaacggtg
    12541 cttcagcctt ttgtcagtca agtgatcatc aagaaaagaa agatcccact gatcaccgtg
    12601 ctgaagacga tgatagcagg attaggagtg attctcttag ctggggtatt gattccgaat
    12661 ttgccgatca ttacttatat cttgggtggg attttagtag tttctatgca atcatttctc
    12721 aatgcgctgg catttgagta ttcaaatacc ggctacagga tcaactttgg cttttcaaga
    12781 gctggtggtt cattttctta tgctgtaact tcttttttac tgggacagct gttagcgatt
    12841 tattctgccg cgattttgcc aatcttggtt atagttgaag cagtgatatt tttcgttatt
    12901 ttgcatctgc tgccggatgt aaaaaatcaa gaagtttttc ctatgacaaa agaacaggat
    12961 cgctcagttt ctttgagaaa aaaatatcct ttcctgattt tgttattttt aggtttcagt
    13021 tttcttttta ctttccacac catgatcaac agctttttgg cacagatctt tgaaaatatc
    13081 cacggcggca gtaaagaagt tggcatcgca ttgatgatcg ccgctttatg cgaattaccg
    13141 ggaatgatct tttttgaaca gcttgtcaaa aagaaatcca gcaaattttg gctgtctgtt
    13201 gccagtattt catttgctgt cagaggattc atgatgctgc tggccagttc gatcctgatg
    13261 atggaaatga cgcatctcct acaagccttc agtttcgctt tgtttattcc agcttcggct
    13321 tatttgttca atcaatttct ggcggatgat gatcgtgtat ttggacaaac gatgatcatc
    13381 atcgccacaa ctcttggtgg ggtgatagga aatgtcttgg gcggcagtct gctgcagcat
    13441 ttcggtgtat gggagatgct gattttcggt actggttttg cggtgctagg tgctttatta
    13501 actattttgg gcattcgaaa aataccgaag acaggaaacg atagtgattc tcttgttgag
    13561 tagcataaaa atacttagat actagtttta tataaataac atatagtgat tattggcgta
    13621 attacaaaaa acgagaataa cttaagtaag catttctaaa taattgagaa acctcatcta
    13681 aaaagataag aaaatatccc taataaaact tctgtaacat tttagataac tgattgtaat
    13741 ctgaataata aactataagg agacggttag acggtctcct ttttttgcgt attataattc
    13801 tagaattgtt ggaaattgat aataattaac aagaaatatt agacaggaac ttcgaactgc
    13861 ttatcacatt cttattatta tatatttgcg taagtagtcc tggtagtctc aatgcactat
    13921 tctatcttga aactaagaag ttatagaagg agcaatgata gaaagcacga gaatacaaat
    13981 agcgattaaa tatttttcta gacaacctta gttaattcac acagatatat tttaggtaca
    14041 gtcttacaga tatagagtta gtgctgtgtg aatcctgatg ttcgcagtgc ataggagtca
    14101 agtaagattc tagccagtat agaaagtgga agtctggagc gtacctcata atctgggaag
    14161 aaggagcctt ctgatttaaa gccaacaaaa ggaatctcga tatgttccat caaacttgaa
    14221 tgacgattag cagtaagcaa aattgtcccg atctcagctt tataacagtt ttcagctgcg
    14281 gtagtcaatt cctttgtttc accgtttagt gaaacaaaga tcacgatgtc atctttggat
    14341 aattttctac taatgttttt tatgatattg ggatcagtat gcatctcaca atatttaccg
    14401 gtaagttgaa atttgaccat catttcctga gcaatcaatt ctgaaaaacc tcgggcaaaa
    14461 atcatgattc ttctagcagc cttgattcgt tgaatagcat cttcaatatt cccgctttct
    14521 accatattga tggtgcggat tacctcttgc tcatttttaa gaatagcagt ctttattcct
    14581 tcatcaactt tatcaagagc ggcaaaattg atatttgtat tttctttttc ttttaacgaa
    14641 tgtttaaaag acgtaaaccc ctcatatcct tttttcttca tagtacgaac gacggttgct
    14701 gttgaaacgt tggcatattc acttagtttt acaatggaat aggaggggat ttcgagtatg
    14761 tgactttgaa taaaatccca gagatatttc tccgattcac ttaattttgt atccatattt
    14821 tgcctccgat tagttaggat aattctattg taaagcggga gtttccttta cttcaagttt
    14881 atttaatata ttttgtaaaa gttttcaatg aatttagtca tttatgaaaa catttacatt
    14941 cgatcttgat acataatgaa tatgtaagaa aggaggagcg taaatgtcag tttatacatg
    15001 tactttaaac ttggcaatcg acctttttat cgaaacagat gaaatgaaac catcccaagt
    15061 caatcgaaca aacgatgatg atatacaagc taatggaaaa ggtgtgaatg tttcattgat
    15121 catgaagcaa ttaggtatcg atagtacagc tttaggattt aaagcaggtt ttaccgggaa
    15181 ttatattgag gattatttgc atcaaaaaga tattagcact gattttatta gcgtaccggg
    15241 aatgactaga atcaacgttt ttacacaagt taataaaacc aatgaagaat tcaaattggt
    15301 taataaagga cctgagattc ctcaggaaca aatcaaaact tttctaaatc aaatacaaaa
    15361 gctgcaagct gacgattatt tgtgtgtttc tggcagtttg ccgcgagggg tctcacccga
    15421 aattcttatt gatatcagtc gtatttgttt tgaaaataag gtccatctga ttattgattc
    15481 aagctatccg gaaataattg attgtctgcc gtatcagccg ttcttattaa aaccaaacga
    15541 ggaggaattg caatcttggt ataatgtcga gcttagtgat cgtgaaggat atattcatta
    15601 tgctgaacgc ttgattgccg atggagcgaa aaatgttctc ctttctctag gaggagaggg
    15661 aggaatattt ttaaataagg atatcgttta ttctgggaat tctcctaaag gtaaagtagt
    15721 caatacagcg tgtgctggtg acactttgtt gggaacattt cttgcaggat atttaaaagg
    15781 tgaacaggta gatattaatt tgaagaaaag tttagcagct ggaagttcaa ccgcttttcg
    15841 taaaggatta acagattttt cggatgttgc agagttggaa aaacaaatcg aaatcaaaga
    15901 gggagcgagt aaaaaatgac aacttacaaa attgttgccg caacaggatg tccaacagga
    15961 attgcccaca cttatatggc tcaagaagca ttggagcagg cagctaagcg caaaggggta
    16021 tctatcaaag ttgaaaccca tggtcaagta gggatagaga atgaattaac accagaagaa
    16081 ataaaaaacg ctgacgctgt aatcattgca gctgacaaag atgttcaagc agaacgtttc
    16141 gcaggtaagc gtgtaatcga tgtgcctgtc agcaagggga ttaaagatgc tgaccaattg
    16201 atcgaagatg cgttggctgg taaaggttca atcactgcag aaaacaaaac agttgacgct
    16261 gctggtgaga aagcggagat gggggttgca gcaggcattg gtcacagtat ttataaaaat
    16321 ctcatgaacg gtgtttctca tatgctgccg tttgtagtgg caggtggggt aattattgca
    16381 atttcgtttg ccatctgggg tgtgtactct tttgatccca atgatcctac ttacaatccg
    16441 actgccgcta tgttaaaggg tgttggtgat gcggcaatgg gaatgatggt tcctatttta
    16501 gcagcttata tcgctgaagg aatcgctaaa cgtcctggtt tggttgttgg gtttgttggc
    16561 ggactgttag ccaattctgg cggagccggt ttcttaggcg gaatttttgc aggtttctta
    16621 gcaggctact ttatcttact attgcaaaag gtcatgaaag gcttgcctaa acaattggat
    16681 ggtctgaagg cgatcttctt atatccggtg atcggtgtct tgtttgttgg tgtagtcatg
    16741 tctttattag caaatccaat ggaagcagtc aaccaaggaa tgatgaattt cttggcagga
    16801 ttccaaaatt ctaatccatt aattttaggg attatcgttg gctgtatgtc agcatttgat
    16861 atgggaggtc cagtcaataa agcggcttat gttacaggga ctgcgttgtt ggcacaaggt
    16921 aaccatacat ttatggcagg ggtttcggca gcttgtatcg cgcccccatt gattactgga
    16981 tttgccgcac tgttttttgg aaaatatttc gacacgaatg atcgcaacgc tggtttagtc
    17041 aactttatct tgggctcaac gcacattacc gaaggagcaa ttccatttgc ggctaaagat
    17101 ccgctgcgtg ttctgccaac tatgatgcta ggttcatcaa ttgctgctgt actgacatat
    17161 atgtttggtg tccaagttcc cgcacctcat ggaggttttc tagtattacc tgttgttact
    17221 ggtaaggttg catgggtagg gtcgatttta cttggttcaa tcgttggcgg agtaatttta
    17281 gggttgatcc aaaaacgtag tgtagaaaaa cgtgaagcag aagcagctgc tgttgaaatg
    17341 aaagaagcaa ccaacaacta aaggttattc tagaaagaag gatttcaaat gactttgatc
    17401 aaaacaaata aagtttttat gaataaaaat tttacgacgc aagatgaggt aatgaacttc
    17461 cttgcagatc agtcaattgt cttgagcatt agtgaagacc gagatgcagt ttacgaaaaa
    17521 ttgatggaaa gagaagaaga aagcacgact ggtatgatgg atggttttgc catccctcat
    17581 gccaaagatg ctagtatcaa ggatgccgat attgtgattg ttcggttaaa cggtaaggtc
    17641 gattggaaaa gtttagatgg agaaggaacg gattttgtaa tcgctctgtt tattccagat
    17701 tcagaggctg gaacaaccca tctaaaattt ttatctcagg tagccagact tttgatgcac
    17761 aaagatgtga cagaaggtct gaaacaagcc cagtctgctg aagaaatagc tgaactttta
    17821 aaccaaaaat tagaagaaaa ctaaaaagga agtagtgaaa tcatgccttt aacagaaaaa
    17881 aagaaaactt atattcgtca actaagtaat gaaagtggca tcatcagtgc gttagcaatc
    17941 gatcaacgtg gtgccttgaa aaaaatgatc ggtaaatttc aaaataaatc agcaaccgat
    18001 gaacaaatca tcaatttcaa aaaaatcatt tcttctgaat tgactccata tgcttcagca
    18061 atcttattag atccagaata tgggttgccg gcagccgaag ttcgcgataa aaatgctggt
    18121 ctgttgttgg cctatgaaaa aactggttac gatgcttcaa ctccaggacg tttaccggat
    18181 attttatcta tttggtctgt aaaacgtcta aaggagcaag gggccgatgc ttgtaagttc
    18241 ttgttatact acgatgtaga tgaatctgat gacatcaatg accaaaagaa ggcgtttatg
    18301 gagcggattg gttctgaatg tgttgctgaa gatttgccat ttttcttaga attggtttct
    18361 tatgatgcca acgatcctga tacttcttcg aaggaatatg ccttgaaaaa accgcataaa
    18421 gtaaatgaaa tgatgaagga attttctaag cctcgctatg gagttgacgt tttgaaagtg
    18481 gaagttcctg ttaatatgaa ctatgtggat ggttacacta aaaatgaagt tgcttatacc
    18541 aaagaagaag ctgctgctta tttcaaagaa caatccgaga ctaccaacct gccatttatt
    18601 ttcttgagcg caggagtaac tgctgaattg ttccaagaaa ccttgaaatt tgcaaaagag
    18661 gctggctcta cattcaatgg ggtactctgt gggcgcgcaa cttgggcgaa tggtgtagaa
    18721 ccattcgtta ctaaaggtga atatgctgcc aaagaatgga tgcagacaca aggacgcaag
    18781 aacatcgaag agctgaataa tgttttgaaa gaaacagcaa cagctattaa attgtaagaa
    18841 agctttaact ataaccttaa gtttgtaacc taatgatttt taataatcaa tttgagcttt
    18901 taaataaaga attggaagcg tcccgacgct tcctttttcg tttgttttta aggcatgata
    18961 ggttctatgt tgtcgttcaa taagcgcaag aaggtcagca tttttttctt agagtatgca
    19021 aaattgcttt tctacccaat gatttaaaac ggagaggcac ccttggatat ggtagatggt
    19081 gaagtaacgc atttcttctg tgatcacggt ttttttgtct tcttgtagcc agatttttaa
    19141 ataattttct tcgatcagat tatttaatct cttttgaaat tggatatctc cgtggttgat
    19201 gacgaataac ttgtatacga acgtattcgt atagacatag tcgatcaatt gagcgtatag
    19261 atcctcgtag ctatgtgtcg tatcgtagga aatgatttca tccaagtctt ggaaaaagct
    19321 attttccagt ttttcataaa gatcatagat atccgtataa tgtgtataaa atgtcgaacg
    19381 gtgaagatca gcagtttcgg tcagttcacg gatggtgatg ttttgaattt ttttatctac
    19441 taataatttt gataatgctt ctgctaacgc tttttctgtt tttttgacgc ggcgatcctc
    19501 ttttttcatt ttgcagcctc ctaatcaaca taatcaagga attgtgtcgg ttatccgaca
    19561 atttcaccgc tatctgaaca tagataaccg acaccttgtc tattataata taagtatagc
    19621 atatcaatat ttaaagaata ggagaataag tgatggaatt agcctttaag caactggaaa
    19681 agaaagacta caaaaaagcc attcaattcg cgattaaagg tatgcacttt gattggtata
    19741 ccaagaacaa actccttttg aatctttatg gaagatactt ttggtatcta gaattgaatc
    19801 gcgcgacaca aatcattgct ttatatgcgg atgatgagtt ggcaggtgtt ttattagcag
    19861 acatcaaagg aaaatcaaaa aagcatcatt catttagtca aaagttatat gttaaactat
    19921 ttgatttctt acaaaatgca tttgtgaaag acagcaaagg cgggtatgac gacaccaatg
    19981 acgaattgct ttcagactat ttaaaaaaac attccccaga tggagaaatt gtctttttag
    20041 ctgccaaccc tgatttaaag atcaagggga tcggtagtaa attattaaaa gaatttgaac
    20101 gccgagagca aggaaaagaa gtatttctgt tcacggatag cggctgtacg tatcaatttt
    20161 atgaacgacg cggtttcgaa cggaaaaaag aaaaagataa cgtcatgaag atccttaata
    20221 aagaagtaga tctccgctgt atgttatata gtaagatcat tcagtaatgt tttttactat
    20281 cagaaagcag aagtaccacg aaggtcaatg gaaaaagcga gtaattgatc catttagatg
    20341 aagataggtg tagtctcaaa gatattagct ggcaaagata tccccagtta aaaatagatt
    20401 tctgtatcca acaataaaaa tatttttgta tttattagta tatatacttt acaaaaattt
    20461 gtatatatac taattttttt gtaggatcaa tgaaatgagg ggaaaatatg ccaaaatata
    20521 aagaaatagc cttggctcta aaagagagga tcgttgaggg aaaatatcag gaaggttcta
    20581 aattacctga gcaagaaagt ctggctagag agtttaaaac aagccgtgtt actatccgca
    20641 aagctataca actccttatt gatgaaggtt tgttatatac gagacgtgga tctggtactt
    20701 ttatacgaag caatatcaga caggacaatg agaatgtcac tcaagtaaat aatgtatttg
    20761 gcacttcctc acaagaaaat acaaatgtga caagtaaaat aatccgtttt gatgtccggt
    20821 ttccgacaga ttatgaaagg cggctttctg caacctactt ccgatcccct tcctgcaaat
    20881 catgggtata aaaaataggc atattaagcg ggctaggagg agaaatattc acatatcatt
    20941 ttttcattcc gcaagctgcg tcaagttatt tggaatttgt agaacagatg agagaggtag
    21001 aagcagcact acaaactact tttcaataag taggaattat agaaatttta ctcaaaaaat
    21061 cttcattaaa aaatctgact aaataagttg agaaaacaga atatacttca atatccagtg
    21121 atttataaat atcattcact catgataccc agctccgtat cataagtgat gcggttttta
    21181 tcattgatcc taaaaatctt agcaagacaa aaagtacgta agaaagtaaa gactaaatac
    21241 ttcggaggaa caaatgaata acaatgatag gttattgcga ttgcgttatg cgattgattt
    21301 gaaagatacg gatttgatta aagcttttga gctaggcggt gtaccactga ctaaagaaga
    21361 agctcaggcg gtactgacca aagtacagga taagaacaaa gataatgcag aaaataatgt
    21421 gtatgaaaaa accatcaaca accaagtttt tgacgcattc ctaaatggcc tgattctctt
    21481 ggcgcgtggt ccgcaaaaag ataagccgat agtagacacg ccgcaaaaaa gcaaggaaat
    21541 caaatatatc aacaatgtgt tgctaaaaaa attaaaaatc gccttgtcta tgaccactga
    21601 tgatatttta gaagtttttg ctgaggcaga actctatcca tcaaaaggcg aaatcggggc
    21661 atttttgaga aaagaaggcc aacgaaattt taagccttgc ggagataaat atatgcgtaa
    21721 ttttctcaag gggttaggaa tttataatcg gagaaaagta taaagaaatt tcatgagaaa
    21781 tcaaaacaat ggttgttttg atgagagcta gtttttgtat gagattaaaa tattatctta
    21841 atcgaggagc tggattggtt ttatgaatga tgaatataag aatgatgaag ataagatgct
    21901 ttttgaagag atagagaatc gatgtaggct caattttgag cttcggggaa agatgtcttt
    21961 aatacaacaa aaaaagtatt tagcgaataa atcagagttt actctgggac atgttgaaaa
    22021 attaatctcg gattggatta gtagtagaag cgaatttaca aaaataaaac agccgattaa
    22081 atttgacatg aaaaaacttt tgttaaataa gagtgagata ggtaatagag atcaatatat
    22141 tagagctaaa gggcaagaaa taattgattc attaggtgaa atgaggtcat acaattattt
    22201 gtatgtaaca catagagctg atggaatggt cattactgtg gggaaatctt cttctaatga
    22261 tattttttta gatggagatc ttttttatca actcaataca aatcacttgt ctggcactga
    22321 aaatataatt ttaaggacgg aatatggcaa tgaaatattt gcaaaatatg atgagatttt
    22381 aaaaaattat ttggactggg cttggataat tccggttgag tcaggagatg ctaagaaact
    22441 ggagagatta ttgggcgatg aactaattaa taaaaaagta ccaattttaa attattattc
    22501 ccatagacaa taaaaggcta tttttcatga taatactagt tataactgta aaaatggaag
    22561 aaaatctggg aaagaagttt ttaaataagt gtattgttcg attttaataa ataataagca
    22621 ataccatccg tctgtgttgc gcaacatcgt actacaatga atattttttc acaaaaagaa
    22681 aatcatccat cgaatgaatg gaaggtttta gtatacacga ggttaatgcc ccctctttta
    22741 taatcagagg agtgaggttt tttctatgaa tgtttctatt ttcttattgc tagtctagtt
    22801 tctcttacga tgaaattggg attctattcc agtttttatt gatttgctga taaaataact
    22861 tgaatggtgg atataaacat gataaataag agaagaataa caaaacaagg ttgtgaaatg
    22921 attctcaaat gaatataata gaaagaaaaa ggaggaagaa tcaatgaaaa tagcaattgc
    22981 aggcgctggc gcaatgggaa gtcgctttgg gttaatgctt caccaaagtg ggaacgaagt
    23041 attattaatc gatggctggg cagaacatgt ccaacaaatc aaagagcatg gattacaagc
    23101 aaattttaat ggtaaagagg tagaagcaaa actaccaatc gttcttcaat ccgaagtaga
    23161 aaaagaagat caagttgatc tgattattct atttaccaaa gcgatgcagc tggaaaaaat
    23221 gctgcaggat atccaatcat taatcaaaaa agatacagag gtcttatgtc tattaaatgg
    23281 tatcgggcat gaagatatta ttgagaaatt tgtaccaatg gaaaatatct atattggaaa
    23341 taccatgtgg acggcaggtc ttgaaggtcc tggtcaggtc aaattatttg gaagtggttc
    23401 agtagaatta caaaatttag gtgatggaaa agaagcagct gcaaaaaaat tagcagacaa
    23461 attgtctgaa tcaggattaa atgctcattt ttctgacaat attcactatt ctatttatcg
    23521 caaagcatgt gtgaacggaa caatgaacgg actatgcaca atcttagacg ttaatatggc
    23581 tgagctagga aaaacatcga ctgctcataa aatggtggcg acgattgtca atgagtttgc
    23641 caaagtagca gcagtagaga agattgaact agatgtccca gaagtcattg cacattgtga
    23701 atcttgtttt gacccggaaa caattgggtt acattatcct tcaatgtatc aagacttgat
    23761 taagaaccat cgattaacag aaatcgatta tatcaatggc gcaatttcta gaaaagggaa
    23821 gaaatatggt gttgcgacgc cttattgtga tttcttgact gagcttgtcc atgcaaaaga
    23881 agatagtttg aacgtaaaat aaaggagaga ttgaatggaa acttcacaaa aaatatcacc
    23941 gaaaattttt ctgaataaag tcctcgctgg tacagcaaca ggaatcatta ttggattgat
    24001 tcctaatgca gtattaggtg ccattttaaa atattttagt gcgtatccct ttgcggtaac
    24061 gatcgcacag ttagcagtta tttttcaact agcaacacct ttgatcattg gtggtttgat
    24121 tgccttacaa tttggatttg atccgatgag aatgatggtt gtagccggag cttcttttgt
    24181 tggttctggt gtagtcaaat tcacacctga tttgggtgaa acgggtgttt acgttggtgc
    24241 tggtacaggc gatattatta atacgatgct gacagcatcg attgctgtcg ggctgatttt
    24301 actgattgga aatagttttg ggtcagtgtc gattgtgtta acaccgattg ttgtaggtgt
    24361 aggtgcaggt ttacttggtt tttatttatt cccttttgta acagcaatta cttctgcaat
    24421 tggatcattg attaataact tcactacctt acaaccgctg ttaatgtcta tcttgattgg
    24481 ttgttccttt gcctttttga ttatctctcc tatttcaaca gtagcaatcg gaatggcaat
    24541 ccaactagat ggtgtttctg ctggtgcggc tgcgatgggc gtagctgcaa ccacttttgt
    24601 tttagtagtg aactcttgga aggttaataa gtcaggtgtg acaattgcga ttgctttagg
    24661 cgctatgaag atgatgatgc ctaatctatt tagaaaacct gtgattctta ttccttgttt
    24721 agttagtgca gtgattacag ctattcctgt cgctctattt tctatttcag gtacgcctgc
    24781 ttctgctggt ttcggcgtgg tcggacttgt tggtccgttg gcttctttag atgcagggct
    24841 aaatggcttt cttgttgttc tttgttggtt agtcatccct gtttttgcgg cactaattac
    24901 tcaattttta tgcgagaaag tatttaaact gtatgatcga gaagaagtct tcaaattttt
    24961 aggctagtag attttactaa gaaattttca atcaatggat gcgtaagtga gatgtcaaca
    25021 acatctgact tacgtttttt tgcctttttg agtaggagat tgatgctgac aactgaactc
    25081 ttcagtgcca ttcgattttt taaaatgtag aagaaactca cctttatgtt attgattgtt
    25141 caaaaggcaa ctagaataaa aatatgtgtt aaaatgagaa aaaatttgat tgttggatgt
    25201 atcgaataat cactaaaaag taggagaaga aaatgatttt tttgcaaaaa tatggttttt
    25261 attttttatt attaggtgtg attagtgatt tatcgacacc gtatatcctt ggcctttttt
    25321 atcccaaact gaatcaaatg actaccgtaa ttagtgtgtt tggtgacgta gatagtccag
    25381 tcagacgtgc tttcttggtc tggtcagttg tatctggact tttttttgtt ctttcgttgc
    25441 ctgcgttgta tcacctattt attggaacat caaaaacact tgcaatactt gctgttgcaa
    25501 cagttggttt atatgggata ggtgattgta tttttactgg gttattcagt atcaatacga
    25561 atgaaagctc ttggaatcta tcgacttgga tccataatat aggttctggg ctaggctatg
    25621 caggctttct acttttccca ttgttacttg ttctgcttta tcgtcaaagt ggtcaaggta
    25681 cgcttagcca tttttatctt gttttaacag ttattagttt actaattgca ggtctttacg
    25741 gattagcacg gattccgtcg attagtcagt ttgccttctt taagcaattg ggcttctttc
    25801 aacgcttaag ttttttcttt aactatttgc cgatgatttg tttaagtttt tttcaactac
    25861 gaaagtagaa aaagcagtgt gaagctgatt aagtaaaaga atttctctat gggtaaccat
    25921 tcgtgtggat gtgttcgact aagaatagag atgaggagag cgaaaagcaa gttagtctta
    25981 acttgttatg cgttgtgcta aatttcaggt agtcattttt tgaatgaata agaagaaaac
    26041 ttgaaagagg gtgacttcat gttagatgtg gagcagatgg aaatagacct ttataatatt
    26101 attttaaatc gaaatacaag agaatgggaa agaacacaat ttgaagaggc aaaagaagac
    26161 ctagcaaaaa tgtcgataaa gcaggtgata gaaaaactag aggtcaactt gcgtccgcta
    26221 gcactacgag ataatttgac accagatgcg atggattttt atttaagaat cacaggggag
    26281 tcagcgagtg caaaggctta tgattatgcc aaacataaga taacttcacc cttataccaa
    26341 gaacgggcga tttttgccgg tggctgtttc tggtgtatgg ttgagccttt tgaaacaaaa
    26401 ccaggtgtaa tctcagtgtt atctggttat acaggtggag agaccaaaaa accaaattac
    26461 gatcaagtca gtggtggata tactgggcat gttgaagctg ttgagatcat atttgataca
    26521 cgtcaaataa cctatgaaga gctgttggag atttattggc aaatttcaga ccctacagat
    26581 gcctttggtc aatttcaaga tcgggggaaa caatatcgtt caattctatt tgttgttaat
    26641 gaaaagcaaa ggcaattggc tgaaaaatca agagaacaac tgattcaatc taaccgattt
    26701 agccgtccta tcgttacaga gattcgccaa gcaaagactt tttggccagc tgaaaatcat
    26761 catcaacaat tttatcagaa gcaacctaaa agatataaaa agatcaagca ttcgcatcag
    26821 cttcatttat tttttcagcg acatagatag aggaaggttg tggcgcaaag cgttcagctc
    26881 cgacaaaata atgagcaact gacaaaaata gcttttcata tttttggaca gttatgaatt
    26941 atttcgagga gttgctttga gaacaccgag gaaaaggttg tggcgcaaag cattcagctt
    27001 caagtcgcaa ggaaacagga ggtatgacaa gtcttttgtc acaactcctg tttttttact
    27061 agcctttatt tccatgtgaa agagaattcg aattgtagaa tcttgatttt tctatggaat
    27121 tttgtagaga atcatcttga taattaatag acacctaatc gaattccaca ttaatgatga
    27181 actaaattcc gtataaaagc gcaaacaata actat
//
LOCUS       DACEKH010000029        26378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEKH010000029 DACEKH010000000
VERSION     DACEKH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.029"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(81..776)
                     /locus_tag="IW662_001159"
     CDS             complement(81..776)
                     /locus_tag="IW662_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ0865995.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(866..1966)
                     /gene="ychF"
                     /locus_tag="IW662_001160"
     CDS             complement(866..1966)
                     /gene="ychF"
                     /locus_tag="IW662_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ0865996.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(1975..2166)
                     /locus_tag="IW662_001161"
     CDS             complement(1975..2166)
                     /locus_tag="IW662_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ0865997.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2187..3077)
                     /locus_tag="IW662_001162"
     CDS             complement(2187..3077)
                     /locus_tag="IW662_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ0865998.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3064..3831)
                     /locus_tag="IW662_001163"
     CDS             complement(3064..3831)
                     /locus_tag="IW662_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0865999.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3843..4556)
                     /gene="rsmG"
                     /locus_tag="IW662_001164"
     CDS             complement(3843..4556)
                     /gene="rsmG"
                     /locus_tag="IW662_001164"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ0866000.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4788..6689)
                     /gene="mnmG"
                     /locus_tag="IW662_001165"
     CDS             complement(4788..6689)
                     /gene="mnmG"
                     /locus_tag="IW662_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ0866001.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6706..8103)
                     /gene="mnmE"
                     /locus_tag="IW662_001166"
     CDS             complement(6706..8103)
                     /gene="mnmE"
                     /locus_tag="IW662_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ0866002.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8447..8785
                     /locus_tag="IW662_001167"
     CDS             8447..8785
                     /locus_tag="IW662_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866003.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(8953..9711)
                     /locus_tag="IW662_001168"
     CDS             complement(8953..9711)
                     /locus_tag="IW662_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0866004.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9729..10547)
                     /locus_tag="IW662_001169"
     CDS             complement(9729..10547)
                     /locus_tag="IW662_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0866005.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10544..10900)
                     /gene="rnpA"
                     /locus_tag="IW662_001170"
     CDS             complement(10544..10900)
                     /gene="rnpA"
                     /locus_tag="IW662_001170"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ0866006.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11018..11152)
                     /gene="rpmH"
                     /locus_tag="IW662_001171"
     CDS             complement(11018..11152)
                     /gene="rpmH"
                     /locus_tag="IW662_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ0866007.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11676..13010
                     /gene="dnaA"
                     /locus_tag="IW662_001172"
     CDS             11676..13010
                     /gene="dnaA"
                     /locus_tag="IW662_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ0866008.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13208..14338
                     /gene="dnaN"
                     /locus_tag="IW662_001173"
     CDS             13208..14338
                     /gene="dnaN"
                     /locus_tag="IW662_001173"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ0866009.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14567..14809
                     /gene="yaaA"
                     /locus_tag="IW662_001174"
     CDS             14567..14809
                     /gene="yaaA"
                     /locus_tag="IW662_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ0866010.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14796..15920
                     /gene="recF"
                     /locus_tag="IW662_001175"
     CDS             14796..15920
                     /gene="recF"
                     /locus_tag="IW662_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ0866011.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15917..17863
                     /gene="gyrB"
                     /locus_tag="IW662_001176"
     CDS             15917..17863
                     /gene="gyrB"
                     /locus_tag="IW662_001176"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ0866012.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17883..20354
                     /gene="gyrA"
                     /locus_tag="IW662_001177"
     CDS             17883..20354
                     /gene="gyrA"
                     /locus_tag="IW662_001177"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ0866013.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20590..20889
                     /locus_tag="IW662_001178"
     CDS             20590..20889
                     /locus_tag="IW662_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ0866014.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20939..21451
                     /gene="ssb"
                     /locus_tag="IW662_001179"
     CDS             20939..21451
                     /gene="ssb"
                     /locus_tag="IW662_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0866015.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21477..21713
                     /gene="rpsR"
                     /locus_tag="IW662_001180"
     CDS             21477..21713
                     /gene="rpsR"
                     /locus_tag="IW662_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ0866016.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21856..23829
                     /locus_tag="IW662_001181"
     CDS             21856..23829
                     /locus_tag="IW662_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ0866017.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23835..24287
                     /gene="rplI"
                     /locus_tag="IW662_001182"
     CDS             23835..24287
                     /gene="rplI"
                     /locus_tag="IW662_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ0866018.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24561..25928
                     /gene="dnaB"
                     /locus_tag="IW662_001183"
     CDS             24561..25928
                     /gene="dnaB"
                     /locus_tag="IW662_001183"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ0866019.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         7999 a         5199 c         5227 g         7953 t
ORIGIN      
        1 attatttaac tatggataaa caaaatgagg ttctttacta cccctagata tctagcaaaa
       61 tagctgatct ttttgctagt ttatcttctt gggacggata aacttccaat aatcccgtat
      121 tttttcttca agtagtaacg gattgctaaa gcagttgccc cgataatgat aactactact
      181 ggatctaata ctggattgat aactggtggc aatagtgttg tcgcagaaaa cagtacgacc
      241 caaagaagtg tcgttccacc taggataagc aatgtcttac ccattcctgg tttcttgctt
      301 cgatctgctc cgggatattc atattgatag atgtatttgt acatcatata aaagacccag
      361 ccaccgattg cagaacctag aacgagtgta gaaaagccat aagctggcgt tgttccacgt
      421 gaaaacaatc cggtaatcgc aatcatgata cttaatagac caagcaacag caacgtatta
      481 tctaaccaca tcaataaagg ggtggatgaa ttttttactt ctgctggctt attgatgatt
      541 gcttccgttc tttcagagac tgtcccaaac aattgacggg ctgttcttcc gcctttttgt
      601 tctgcaacta aagtaggcag aatgtcatga agggctttta ctttttcttc ttcagacaaa
      661 ttcgctgctt ctaaagattt tttcaaatca aaaatgtact gttgatttct tttggttaat
      721 tttgtttcta aactattgtt ttcttttacg attgctcgta attgttctgg ttccattatc
      781 taatagtcct ccttagccgt tccttttctc aacggtcgtc acaaaagaca ttctaccata
      841 ttctacagct agtttgatag cccttttaca cattaaagcg gaataacatt acgtcgccgt
      901 cttgtacgac ataatctttt ccttctaatc gaacacgtcc agcttctttt gctgcatgca
      961 tgtttccgta atgatccaag tcttcaaaag agacagtttc tgcacggata aatccgcgtt
     1021 cgaaatctga atggatgatt cctgctgctt gtggtgcttt gattcctttt ttgaatgtcc
     1081 aagcacgtac ttcttgttcc ccagcagtaa agtaagtagc taagcctaac aaatcataag
     1141 cagcacggat caactgatcc aaacctgatt cttcgattcc taaagcttct aaaaattccg
     1201 ctttgtcttc ttcatctaat tctgcgattt cttcttctgc acgagcactg accacgatca
     1261 cttctgcatt ttcttctgca gcaaagttgc gcacttcttg tacataatga ttcgcgtctg
     1321 catctgctac gtcatcttct gagacattgg ctacataaag aattggtttt gtcgtcaata
     1381 aaaatagtga tttaacgatt ttttgttctt cttcagtaaa ttcgatcgtt cgggcagatt
     1441 ttccttcttc cagtacaggt ttgattttat ccagtacagc tagttctgcc actgcatctt
     1501 tatctttggt tttagcaact tttgctacac gagtatagcg tttgttgata gattctaagt
     1561 ctgctaacac tagttccaag ttgatcgtat caatatcttc tagtggatca acacgtcctt
     1621 ctacgtgagt aatattatca tcatcaaaac agcgaacaac gtgacaaata gcgtctactt
     1681 ggcggatatg gcttaagaat tgatttccca aaccttctcc tttacttgcg ccttttacga
     1741 ttccagcaat atctgtaaat tcaaaagtcg tcggaacagt ttttttcgga tgtacaagtt
     1801 ctgttaagcg ttgcaaacgc cagtcaggta cttctaccat ccctacgttg gggtcgatcg
     1861 tcgcaaaggg atagtttgct gcttctgctc ctgcttttgt gatcgcatta aacaaagttg
     1921 attttcctac gttgggcaac ccaacaattc cagcagtcaa tgccataaat ttattcactc
     1981 tttctctgca tttttttcaa ttactttttt taatttcttt tcaaaatctc gacgggtcat
     2041 catgacgata tgtccgcaat tcatacatct gattttgata tctgcgccca ttcgaatgac
     2101 ttcccagcga ttcgtctggc aagcgtgagg ttttttcatt tcaacgatat cgtgcaagtc
     2161 gtacataaaa atagcctcct ttttgtttat tcttcatcaa attgaatatt caagatatca
     2221 agaattcgag tcaaatcttt ttgagaaaga tattcaatct cgatcttacc tttcccattt
     2281 ttttcttgga tagctacact tgtaccaaat ttatccatca aacgatcttc gctttctcgg
     2341 atataatatg gtttttcctt tgctagcctt gggatttttt tcttttcttt tccatcattt
     2401 tgatttaact cagaaacaag ctgttccagt tggcggacag ttaaattttc agttactgca
     2461 cgattggcta atttcaagat caatgtttta ttttttaaac ctaacagcgt acgggcttgt
     2521 cccatagata aacgctgatc ttgtaccatt tccttgacaa gatccggtaa agaaagcaag
     2581 cgcaaataat tggcgatata aggtctgctc tttcctaaac gactagctac ttctgcttgt
     2641 gttagtttca ggtttttcat cagcatctca tatgcttctg cttcttctaa cggattcaga
     2701 tcttctcgct gcaagttttc cagcactgct acttgcatca tcgcttcttc gtcaaagtct
     2761 ctaacgatag cagggatcgt ttctttttca gctagtttgg acgctcgaaa acgtcgctcc
     2821 cctgcaataa tctcatatcc ttttactgcg gaacggcgta cgatgatggg ttgaaagaca
     2881 cctgattgtt caatagaatt agctagttcc tgcaaagata cttcttcaaa tgttttacgg
     2941 ggctgatacg gatttggtcg cagctcattc aatggtagct ggacaacggt atcatctttt
     3001 acatccaccg tctctaagtt ttctaaatct tgaaacagtg catcgattcc tcggcctaat
     3061 cctttacttt tcttcacgac ttaccacttc ctttgccaat gcttggtaga cttcagctcc
     3121 tcgagaacgt ggatcgtagt cgatgatcgg ttttccatga ctaggtgctt ctgataaacg
     3181 aatatttctt ggaatgatcg tttcgtaaac tttttcgcgg aagtatttac gtacttcttc
     3241 tacgacttca tttcccagat ttgttcgtgc atcatacatt gtcagtaaga ctccctcgat
     3301 ctctaattct ggattaaagt gtttttggac taaacgaacc gtattcagta attggcttaa
     3361 tccttccaat gcgtaatact cacactgaac aggtatcaaa atcgaatcac ttgctgtaaa
     3421 tgaattgatt gtcagatggc caagagaagg cgggcaatcg atcaaaatat aatcatactg
     3481 actgcttact tctgctaaag atccttttaa tcttgattct cttgccatca ttgacgtcaa
     3541 ttcgatctct gctcctgcta attggagagt cgcaggcaca atactcaaat tttcgtgttc
     3601 agtgatcaaa gttgcctcat caattggcaa ttcattgact agaacatcat agatgtctct
     3661 tgtcacatca ggttttctga tcccgacacc actggtagca tttccttgtg catccatatc
     3721 cactagcaat acttttttgc caaggctagc aagacaagca cctagattca ccgtcgtcgt
     3781 tgttttgccg acgccacctt tttggttcgc aacagatatt atttgtgcca tctcttctcc
     3841 tcctatttga ttggctgctt attaggcagt ccgggctttc ttgggtattt ctttggggtt
     3901 ggtttctttt tttgaataac gatgatatgc cgttgatcag agacattcgg caaggtaacc
     3961 gcctcttctt tgaggaattt accacctaat aaagcaatcg cttgttttgc ttctgtcaat
     4021 tcctcttcac tttttgctgc tttcaatgca taaaaatagc cttcttgttt agccaagggc
     4081 aaacacaatt ccgctaacac attcaaacga gcaaccgcac gagcagtgac aaggtcaaat
     4141 gatgcacgaa agtgaggatt ttgcccaaat gtttctgccc ggtcatgata taaggaagca
     4201 tcgattccta ataatcgggt taactcagat aagaaggtta ttcgtttatt cagagaatca
     4261 acgatcgtta tctgcagttc tggaaaggca atttttaaag gtatgctagg aaaccctgcc
     4321 cccgaaccaa catcgcataa agataaaggt tgcgtaaagt ctacttcaaa tgctaatgta
     4381 agtgaatcgt aaaaatgctt cagatatacg tctttttcat ccgtaatagc tgtaaggttc
     4441 atcttttcgt tccattcgac taatgtccga taataaatag cgaattgatt catttgatga
     4501 tcggtaagct ctatcccttt ttctgccaag gattggcgaa attcttctgg tatcataaat
     4561 attcctttca ttgtgaaaca gcaattgttt cacagattct ctccctactt taacgcaaaa
     4621 tctctagaca tgcaatcctt ccatatggat tcttaaaaat ttgaaaagta agaaaagccg
     4681 atagactttt tctttctagt atgactcata aaatatacac agaagagaga actagctttg
     4741 agcaatttct gcaaaaacta gttctctctt ctatttatta ttacgtatta cgctggttca
     4801 ctgactttcg caatcttccc ttgttcgata tagaccatta ggatacttag gtctgcaggg
     4861 tttacgccac tgattcggct agcttgggca attgtttctg gttggatttt ctgcaatttt
     4921 tgttttgctt ctgttgctaa tccgttgatt gctgcgtagt cgatgttttc tggaatcttt
     4981 ttcgcttcca tgcgttttaa tttttctact ttttccatgg cttttttgat gtagccttca
     5041 tatttgattt ggatttctac ttgttcgatt tcttttcgtg tcaattcttc attttctgga
     5101 ataaaccgta gaagttctgc ataagtaatc tcaggacgtc ttaaaaagtc gcttgctagt
     5161 acaccatctt ttaatggtgc cgcattaatc gttgctaaaa agtcttggac ttcttctgtt
     5221 ggtttgattc gtacattcat caaacgtttg atttcttgtt caactgcttc ttttttcttc
     5281 aaataattct tgtattgatc ttcttttact aagcctacac ggtgacccat ttctgttaat
     5341 cgcaaatctg cattatcatg acgtaaaagc agacggtatt cagcacgtga agtcagcaaa
     5401 cgataaggtt cgttcgttcc ttttgttacc aaatcatcga tcatgacacc gatgtaaccg
     5461 tcacttcgtt tgatcactaa aggttctttt ccctgaactt tcaaagctgc attgataccg
     5521 gcaatcagcc cttgtccagc cgcttcttca taaccgcttg taccatttgt ttgtccagct
     5581 gtataaagat tttcaacgac tttagtttct aatgtcggac ggagctggtg tggctctact
     5641 acgtcatatt cgatagcata tcctgtacgc atcatttcta ctttttctaa tccagcaata
     5701 gaatgaagca tgttgacttg aacatcctct ggtaaagaag tagataaacc ttgaacatag
     5761 acttcttccg tatttaagcc ttctggttcc aaaaataatt gatgacgtgg tttgtctgca
     5821 aaacgcacga tcttatcttc gatggatgga caatagcgag cgcctacccc ttcaacgatc
     5881 cctgtaaaca ttggggcacg atgtaagttg tctcgaataa tttggtgagt cgtctcacct
     5941 gtataggtca gccagcaaga ttcctgatct agtttatagg cactatctgg tgtgctgaag
     6001 ctaaaatgat tcggctcttt gtcaccaggc tgctcttctg taacagaata gtcgatcgta
     6061 cttgatttta ctcgaggcgg tgttcctgtt ttgaaccgtt cgatagcaaa gccaagttct
     6121 tttagatgat tcgctagtcc aatagaaggt tgagagttat tcggacctga tgaatatttc
     6181 aattcaccga taatgatttc tccgcgaagt gctgttccgg ctgtaataac aactgctttg
     6241 gcccggtagc tcgctcctgt ggacgtcact actccatgac aaacaccatc ttcgacgatc
     6301 agttcttcga cgattccttg tcttagcgtc aaatttggtt ctttttcgat cgtatgtttc
     6361 atttctgttg aataagcatg tttgtctgct tgtgctcgca atgctcggac agctgggcct
     6421 tttcctgtgt tcaacatacg catttggata taggttttat cgatattctt acccatttct
     6481 ccgcctaacg catcgatttc tcgaacgaca acgcctttag ctggtccgcc tacagaagga
     6541 ttacatggca tgaatgctac catgtccaaa tttattgtca gtaataatgt ttttgatccc
     6601 atacgtgcag cagcaagtgc agcttctgaa ccggcgtgtc ctgcgccaac aacgatcacg
     6661 tcatacgcat ctgcttcata atgttccaat cgtttgttcc tccttttatt ttcctaaaca
     6721 aaattgactg aataattgag tgattagctc atcttgtaca ctgtcgccga caacttctcc
     6781 tagatagtcc cagcaacgcg tcatatctat ttgaacgaga tccacaggca ttcctgaatc
     6841 aatacctgca atgacttctt caagtgagag ggaagctttt tctaacaacg cgatatggcg
     6901 ggtattagaa acataagtag cgtctctttc tcctgtttct ccgctgaaga ataaatcaga
     6961 aatagctgat tccaaacgat ctagtccgtc gtttttagct acggaaattg aaaaaactgg
     7021 ttcattttcg atcaatttct tcaatttttc ttgttccagc tgtgctggca aatctgtttt
     7081 atttagaaga atgatccgtt tcaatccact tgtcgcttcc aataattgtt catcttctgc
     7141 tgtcagtggt tcgctttggt ttagaacaag taagatcaag tctgcttctg ctaatgcttt
     7201 tcggcttctt tcgaccccga tccgttccac cacatcttct gtttcccgaa ttccagctgt
     7261 atcgattagc ttgagcggta ccccccgaac attgacatac tcttcgatta catctcgagt
     7321 cgttcctgca atatccgtaa cgattgcttt ttcttctctt aacaaatggt ttaacaaact
     7381 tgatttgccg acatttggcc ggccgatgat tgccgtactt aacccttccc gcagaacctt
     7441 cccttgcttg gatgtagcaa gtagcgcttg gattctttgt tggatcatct gcgctttttc
     7501 aagcaataac ttcgtagtca attcttcgac atcatcatat tctggataat caatatttac
     7561 ttctacttgt gctaatgtct ctaaaatctc ttgtctcaat gagcggatca aagcagacag
     7621 attgccgtct aactgattaa gagctagtcc cattgctttg tctgtttttg cccgaatcaa
     7681 atccatcaca gcttctgcct gtgaaaggtc aacacgacca ttcaagaatg ctcgttttgt
     7741 gaattcgcct ggctcagcta atcttgcacc ttctcttaac aatagctgca aaatttgatt
     7801 gactacaacg atcccaccgt gacaattgat ttctactaca tcttcccgcg taaatgtctt
     7861 tggtgctctc atcacggaga ccatgacttc atcgacaagc tgattgctct tgggatcaac
     7921 gatatgtcca taatggatcg tatgacttgg tacttctgat aatcgtttgt ttccagattg
     7981 ataaacttta tctgctagtt gcacagcttg atcgccactt aaacgaacga tactgattgc
     8041 accttctcca ggtggggtag aaattgccgc aatcgtatca aattctaaag taatttcagc
     8101 cattttttca cttccttttt cattttgcgc acaaaaaaag tgcccactcc accctttatg
     8161 cacaataaac ggtgaacttc gcactttgac tgcttgttct tgattaaatt ctggtcatag
     8221 attatcacac ttgctataag gaatcaagtg tttaaaacaa ttagatgaag ttgagcctgt
     8281 tctgcttcca ttaccttata tttaattgtc cttggatgtc tctttgcgtt ctttggttta
     8341 ttcgaagaga agatactgta taaacaaggc tttatctttc ttaaaaaaaa cggtacacta
     8401 aatggtagcg ctatctaaac tatacacttt tgaaaggaga aaatatgtga gcccgttcag
     8461 atctgctgca ttggaagaag aaagtgaccc tcggatcaac gatgtcgttt tatctacagg
     8521 gaatatcaac cagaaatata tcgtccgcga tatcgttttt gttgtggaat gcttagaagc
     8581 tgatttgttt gacccatctt ttcccaaaga tgaccttttc ttaacaacga agaggaaatt
     8641 aaagacgaag gtacttgaat atggtgcgaa tgcagtaatc aactgtcatt ttgagtatca
     8701 gcaaaaaaaa cgcgacggaa agtattttct ggaacttttt gcttatggaa cagtgatcca
     8761 atttatccaa acaacgattg gttaaatgaa acagacaaaa aaagcggatc aaaactgaag
     8821 acagtttcga tccgcttttt tctatcaaag ttagtgacta cttcatcgta tactctgata
     8881 agaatgattt ctagtaaaat atacttggtt tgaaaaactt tgtcaacgtg atttattcaa
     8941 aaagtaatgg aatcaataat attttttagc aggttctact actaaataac gataaggttc
     9001 ctcaccttcc gaatgtgttt ttacatgttc atctttgctt aatgcagcat ggatttgttt
     9061 acgttcaaat gcaggcattg gttctaagaa aacaggctgc cctgtccgtt ttactttttc
     9121 agccgtgcga tcagctaaac gctgaatgat ctcttggcgc ttttcacggt aattgcctac
     9181 attgactact acagacagct tatttcttgc taaacgatgg atgaagactt gtgccagata
     9241 ttgcaatgca ttcagtgttt ttccatgttt cccaatcagt atgccttgct tttgtgtttc
     9301 taaatggaaa acaaccatat tatttgattc tctttccatt ttgacaagag ctggagcatc
     9361 aagctgtttt gaaatttcag taagataaac tgccaattcc gttaaagctg cttcatcacc
     9421 tagctcactt acttcttctg ttttttcttc aagaatcgtt tcgttgacta cctcgatctc
     9481 agctgttggt tcttcttcca aaatttcttc tacagtctgt tctactgttt cagaaacttt
     9541 ttcgctgata cttggctgga tcgaaagccg tgcattcttt cgacccattc ctaggaaacc
     9601 tttttttcct tcttccagta tttcgatttc gacttggtct ttggccaaac ccaaatcagc
     9661 cagacctagt tggacagctt cgtctattgt tgcagcttca taaatcggca ttagcattac
     9721 ctcccctatt atttccgttt tttcttagga ttcattgctt tcttaagagc acgttctctt
     9781 tcgcggattc tccgtgcttc ttcttcacgc tcttttcgaa ttttgaaagg gttgttgatc
     9841 agcagcgttt gtacaacttg gaaggcattg gaaactaccc agtataatga aagaccgctg
     9901 gctaaattga cacccatcac taaaatcatt acaggcatca cgaaattcat gatcttcatt
     9961 gatgcattcg attcaatttg actcatgcta gataaatacg tacttgcaaa agtaaagatt
    10021 gctgctaaaa tcggcaaaat aaatgttgga tccgcgttcc ctaagttcaa ccataagaaa
    10081 tgcccttgtt tcaactctgg tacacgtgaa atagattgcc ataacgccat caagattggc
    10141 atttgtacta ataaaggaag acaaccagca tatggattga cgccattttc tgcatataaa
    10201 cgctgctgct cttctcttaa cttactttgt gtttccggat ctcttgaact atattgttgc
    10261 tgcagtgctt ttaatttcgg ttgtaattct tgcgttttac gcatgctctt tgtttggaaa
    10321 tgcatcaacg gcaataggat cacgcggatg atcagtgtaa ataaaatgat ccctatccct
    10381 gcattaccaa atgacaacgc cttgatcgcc tcagcaaagt aataaacaat ataacgatcc
    10441 caaattcctg tactctgtgc gctgacttcc ccagttccgc aaccggataa gacgatgacc
    10501 aaactaacga taccggccat caaaagtaaa cgtttgtatt tcttcactta ctcttttcct
    10561 ctctgtcgat tatttttgca agttttaaga cgtggactaa attggagcga acctcatcat
    10621 aagtcaaccc cttcacactt ggtctagcaa tcacaataaa atccaactcc ggatcaattt
    10681 cttcttttat agaataaact gctgctcgga tttttcgttt gacttcattg cggtttaccg
    10741 cgttgccgac cttttttcct acagataaac caactcgaaa atgaggctgt cccggttttt
    10801 ccaaacggta aacgacgaat ttgcggttag caaaagaatc tcccgtatgg aacacggctt
    10861 gaaactctcg ttcttttttt actcgatacg cttttctcat tagttttctt tccttttcta
    10921 tctctcactt acgctcataa tcataccata attacgattg tcctttcatc atttccctaa
    10981 aaaagagtat aaaaaaaacc actgaaaccc cagtggccta agctgaaaga acttttcttc
    11041 ctttgcgacg acggctagct aacacacgac gtccattttt agtactcata cgtttacgga
    11101 atccgtgaac tttttgacgt ttacgtttat ttggttggta tgttcttttc atatattcca
    11161 cctccaaaat gttcttatct attttataaa cacagacata ctttgatagt ataacgacaa
    11221 agtagtgatt ttgtcaatat aaaatagaag atcgattgtt aaaacttatc cacaagatta
    11281 tttttttcta tgggagtcct atgtggaaaa agttatgcac agggattatc cacagataat
    11341 aaatggctgg ttttacacag aaaacacacg tgtaaaaaaa gtaatccaca atcaggtgat
    11401 ttcctgtgta ttccgtctcc taactattgg tatatctata ttttttatcc acagatgcaa
    11461 gaggaaatct attgattttt acgagttttc cacctgtgca ttagtggtgt ggaaaacttt
    11521 tttttgacaa ctgtgttttt ctcttttttc tttttgtcga ttgtgtggat aattgtaatt
    11581 aaaaaatttt tttcttcggt ttctgtggaa aactattgaa aaaaatgcta aattagtact
    11641 agttattcac tttttaatga aggaggaggc cttatatggt atccctcgat gctttatgga
    11701 atgaattaaa agcaacatac caaaaggatt tatcgcctgc tagctataat acatggatcg
    11761 aaacagcgaa ccctcgaacc cttgatcaaa accagctcgt tgttgaggtt cctagcaaga
    11821 ttcataaaga atattgggaa aaaaacctgg cgacaaaaat cgttgaagtc ggttatatgt
    11881 tatcaggaaa tgagattatc cctcgcttta ttactggcga agaagcagag caagaagaag
    11941 ttatagaaga aaaaaatcca aaagttgtgg cacctagtcc gctaaaaaaa gccatgctga
    12001 accctaaata tacctttgat acgtttgtca ttggtaaagg aaaccagatg gctcatgcag
    12061 cagcgctcgt tgtagcagaa gatccaggtt ctatttataa cccgctcttc ttttatggag
    12121 gcgtgggact tggtaagact cacttgatgc atgcaattgg tcatcaaatg ctgcaaagtc
    12181 agcctaatgc caaagtaaaa tatgtaagca gtgaaacttt tgctaatgat ttcatcaatt
    12241 cgatccaaaa caaaacagca gaagagttcc gtcaagaata tcgaaatgtt gatttactgc
    12301 tagtggatga cattcaattt tttgcagaaa aagaagctac gcaagaagag tttttccata
    12361 ctttcaatgc cttgtacaat gaagggaaac aaatcgtact aaccagtgat cggttgccaa
    12421 acgagattcc gaaactgcaa gaacgcctcg tttctcgctt tgcatggggg ctgtctgttg
    12481 atattacacc gccggatctt gaaacaagaa cagcgatttt acgtaaaaaa gcggcggctg
    12541 aacgtctaga gatcccagat gacacactaa gctacatcgc aggacaaatt gattctaaca
    12601 ttcgtgaatt agaaggtgcg cttgttcgag tgcaagcttt tgctgcgatg aatagtgaag
    12661 atatttcgac tagtctagct gcagacgcac tgaaaacgtt aaaatcagga aaaggacatc
    12721 ctcaattgtc aatcctgcag atccaagaag aagtggcaaa gtattatcac atccaattga
    12781 aagatatcaa aggaaagaaa cgcgtcaaat ctattgttgt tcctagacaa atcgctatgt
    12841 atttggcacg tgagctcaca gataattctt taccgaaaat cggagctgaa ttcggtggca
    12901 aggatcatac aacggttatc catgctcatg aaaaaatcca gcagctgatg gattctagtg
    12961 tcagtatgca aaatgaagta tcagaaatca aaaacttttt actaaattaa tttgtcgatt
    13021 ctatcaactg aaaatagcat gtggataaaa gaaaaaaacg ttcaaaagtt atgcacactt
    13081 tttccacagc tgttttttcg tttctctctt acgttttttg agttttccac agaatcaaca
    13141 tgccctatta cttttattat tctttttaac taataataaa taaacaaaca ttcaaaggag
    13201 atacatcatg aaagttactt taaaccgagc tagctttatg caggaattgc aaactgttca
    13261 acgagctatt tcaagcaaaa ccacgatccc tattttgaca ggtgtaaaaa tcacactgac
    13321 acaagaaggt ttgactttga cggggagcaa cgctgatata tcaattgaaa cttttttgtc
    13381 tgttgaaaac gaaaaagcaa atatgcaaat cgaatctact ggttccattg ttttacaagc
    13441 acgtttcttt agcgaaatca ttcggagact tcctgaagaa acatttactt tagaagtttt
    13501 agaaaataaa caagtagcga ttacttctgg aaaagcgaat tttatcgtaa atggattaga
    13561 tgcagataac tatcctcatc ttcctgttgt cgaaagccat aaccagatga aattacctgt
    13621 acacgtattg actaaactaa tcaacgaaac agtttttgct gtctcacaac atgagagtcg
    13681 tccaatcttg acaggtgtgc atttcatttt atctgataat tctttattgg ctgtagctac
    13741 tgattctcac cgtctaagtc aacgcgtgat tccagtagaa caagcggctg atcattttga
    13801 tattgttatt cctggaaaaa gtttgatcga attatctcgc tcattaacaa atgaagaaga
    13861 aatcgtcgaa atcagcatta tggaaaacca agtgttgttc aaaacagaaa cgatgtattt
    13921 ctattcccgt ttgctagaag gaaactatcc tgataccaat cgtttgattc cttcaagttt
    13981 taatacagag gttgaatttt ctgttcctag ctttttagcg gcgatcgaac gggcttcttt
    14041 actttctcat gaaggccgta acaacatcgt tcgtttatct attcgtccag atgcggttgt
    14101 tttatacgga aattcgccag aaatcgggaa agtagaagaa agcttgagtt atacagcaag
    14161 cagtggcgat ccattagata tttctttcaa tccggattat atgaaagcag cgttgcgtgc
    14221 atttggagat atgagcatca aagtgaaatt tatctctgct attcgtccat ttacattgga
    14281 accaacggaa gatggcgttc aatttattca gctgattact cctgtacgta caaattaaca
    14341 atgattttca ttgttcttga aaatttaatt gaaaagaaat agaaaaaata aaattttaaa
    14401 cagaaggccc taaaatctct tttaaggctt tctgtttatt tttgtttaaa tcaccttttt
    14461 tagattaaat cgtcttatat ggaaaaatat atggataaat ttgttttatt gcgaaaaaaa
    14521 aggtataata gagtatactt ttaataatga aaatgaggga tgggcattga aacaacaagt
    14581 cattctaaat acggaattca tgacgttggg ccaaatgttg aaagaagtca cagttatcgg
    14641 tagtggcgga caagcaaaat ggtatttggc agaaaacact gtgttagtcg atggcgaacc
    14701 agaaaatcgt cgtggacgca aactttatcc cggcatgatg gttgaagttc cagaagtagg
    14761 aacttttttt atggtaaaaa aaggaaacga cgataatgag gctgaataag ctgtatttaa
    14821 aaaattatcg aaattacgaa gagctgaaca tcgaattttc caagaacctc gtcatctttt
    14881 taggagaaaa tgcgcaagga aaaacgaatc ttttggaaag tatctacgta ttagctatga
    14941 cgcgaagtca tcggacaagt agtgagcagg aattgattgg ctggactgac gatcaggcga
    15001 tgattcaagg agagatcaca aaaggttcat ccactcttcc attagaaatc ttattatcta
    15061 aaaaaggccg aaaaaccaag atcaaccata tcgaacaaaa aaagttaagt acttacgtgg
    15121 gacaaatgaa tgtcatttta tttgcaccag aagatctttc tttagtaaaa ggcagcccgc
    15181 agatcaggcg gaagttttta gatatggaat taggccagat caatccaatc tatctttatg
    15241 atcttgtcca ataccaagca attttgaaac aaagaaacca gtatttaaag caattgaatg
    15301 aaaaaaaaca aactgatttg ctttatttgg atattttgtc cgaacaacta gcagcgtttg
    15361 gcagcaaagt attgaaagca agagcacagt tcgtcaagcg attggagttt tgggctaatt
    15421 ctttgcatca gcaaatcacc catcaaaaag agcagctgga aatcgagtat ctcaccgcag
    15481 tcgattcttt agaaacacat acgcaagagc aaatccaaga acaatttctc gcacttttga
    15541 accaaaataa aaagaaagac ctttttcggg gaacaacgac agttggtcct catcgtgacg
    15601 atctttcctt ttttattaat cagaaaaatg tgcagactta tggatcgcaa ggacagcaaa
    15661 gaacaactgc actaagtgtc aaattagcag aaatcgattt gattaaagaa gagacggggg
    15721 aatatccaat ccttttgtta gatgatgtga tgagcgagct ggatgatagt cgtcaactcc
    15781 atctgctaga aacaatcgaa ggaaaagttc aaacgttctt aacaacaacg acacttgacc
    15841 atgtaaaaaa taaaatgact gttgaacctg agatttttta tgttcaacaa gggaaactaa
    15901 aaggagaaga aactgaatga cagaagaaag aagtttagta gaacgcgcta aagagtatga
    15961 tgccagtcag attcaagtac ttgaaggact tgaagccgtt cgtaaacgac ctggcatgta
    16021 tatcggttcg actagttctg agggtctgca tcaccttgtt tgggaaattg tcgataattc
    16081 gatcgatgaa gtattagcag gttttgcaac gaaaatccat gtgattattg aaaaagacaa
    16141 cagtatcacc gttattgacg acggtcgggg gatacctgta gatatccagg caaaaactgg
    16201 acgtcctgca gttgaaacgg tctttactgt tttacatgct ggaggaaaat tcggcggtgg
    16261 tgggtacaaa gtttctggtg gacttcacgg agtaggatct tctgttgtta acgcgctttc
    16321 tacacagctt gatgtcaaag tatacaaaga aggaaatgtg tactatcaag aatatcgtcg
    16381 tggagctgta gtggacgact taaagattat cgaacaaacg gatcgccatg gaacaacggt
    16441 tcactttacg ccagaccctg aaatttttac agagacaact gagtttgatt tttcaaaatt
    16501 ggctacccgg atccgtgaac ttgctttctt gaaccgcggg atgcgtatat cgattgaaga
    16561 caaacgagaa gaagaaccag tcatcaatga ataccattat gacggcggga ttaaaagtta
    16621 tgtcgaatac ttgaatgcaa acaaaaccgt gatttttcca gaacctgtat atttggaagg
    16681 tgaacagcaa gatatcgcag ttgaagtctc tatgcaatat acagacggct accattccaa
    16741 tatcatgagt tttgccaaca atatccatac gtatgaaggc ggaacacatg aatcaggatt
    16801 caagactgct ttaactcgtg tgataaatga ttatgcccga aaacaaaagc tgatgaaaga
    16861 aaatgacgac aacttgaccg gtgaagacgt gcgagaagga ttgacagctg ttatttctat
    16921 caagcatcct gaccctcaat tcgaaggaca aacaaaaaca aaattgggca attccgaagt
    16981 acggacagtc actgatcggt tatttagcga acatttcatg aaattcttgc tggaaaaccc
    17041 aagtgtgggc cgtcaaattg ttgaaaaagg attactagct tccagagctc gtctagcagc
    17101 gaaacgtgcg cgtgaagtaa cgagacgtaa aggtgcttta gagattagta atctgccagg
    17161 aaaattagcg gactgctcta gcaacgatcc tgaaaaatgc gaattgttca tcgtcgaagg
    17221 agattcggcc ggcggatcag ctaagcaagg tcgtaatcga gagtttcaag ccatcttgcc
    17281 tattcgtggg aaaatcttga atgttgaaaa agcaagcatg gacaagatct tggcaaatga
    17341 agagatccgg tctttattta ctgctatggg tacagggttt ggagaagatt ttgatgtatc
    17401 gaaagctcgc taccacaaat tagttatcat gactgatgcc gatgtcgacg gcgcgcatat
    17461 ccgtacattg ctgctgactc ttttttatcg ttacatgcgt cctattgttg aagctggata
    17521 cgtctatatt gctcagccac ctttatacgg ggtaaaacaa gggaagaata ttacgtatgt
    17581 acaaccagga aaaaatgcag aagaagagtt gaaacaagtc gttgcttcac ttcctgcctc
    17641 accaaaacca agcgttcagc gttataaagg acttggagaa atggatgatc atcaactatg
    17701 ggaaacaact atggacccaa gcaatcggat gatggcccgc gtcagtgtgg acgatgctat
    17761 tgcagcagat cagatatttg aaatgttgat gggggatcga gtagaacccc gtcgtgcatt
    17821 catcgaggaa aatgcacact atgtgaagaa cttagatatt taatcatgaa ggaggaaaaa
    17881 gaatgagtga agaaatcaga gaaaacatcc atgatgtcaa tctaaccagc gaaatgaaag
    17941 actccttcat cgattatgcg atgagtgtta ttgtcgcacg tgcacttcct gatgtcagag
    18001 atggtctgaa acccgttcat cgccgtattt tatacggcat gaacgaatta ggtgtaacac
    18061 cggataaagc ccataaaaaa tctgccagaa tcgtcggaga tgtcatgggt aaataccatc
    18121 ctcatggaga ctatgcgatc tatgagtcaa tggtacggat ggcacagcca ttcagctacc
    18181 gttatatgct agtggatggt catggaaact ttggttctgt tgacggtgac ggtgccgccg
    18241 ctatgcgtta taccgaagca cggatgagca aaatcgctac agaaatgctg cgtgatatca
    18301 ataagaacac tgttgatttc caaagcaact atgatgatac agaaaaagaa ccagttgtac
    18361 tacctgctcg cttcccgaat cttttagtga acggaacaac ggggatcgcc gtaggtatgg
    18421 ccacgaatat cccacctcat aacttgtctg aggtagtagc ggctatcgat ttgctgatgg
    18481 ataatcctga tgtgacgacc aatgaattga tggaagtatt acctggacca gattttccaa
    18541 caggtggttt ggttatggga aaatcaggaa tccgaagagc gtatgaaaca ggtaagggtt
    18601 ctattaccgt acgtgcaaaa gttgagatta ctgagatgcc aaatggtaaa gaacggatca
    18661 tcgtgacaga attgccttat atggtcaata aagcaaaatt gatcgaacgg atttctgaac
    18721 tgcacagaga caagcgtatc gaagggatca cagatttgcg ggacgaatct tcccgtgaag
    18781 gtatgcggat cgtcattgac atccgtcgtg acgccagcgc atctgttatc ttgaataact
    18841 tatataaatt gacatccttg caaaattcat ttggattcaa tatgttggcg atcgaaaaag
    18901 gtgtgccgaa agttttgagc ttgaaacaaa ttcttgaaaa ctatatcgaa catcagcgcg
    18961 aagtcatcac aagacggaca gtctttgaaa aagaaaaagc agaagcaagg gcacacattt
    19021 tagaaggttt gcgtattgct ttggatcata tcgatgaaat cattgcgatt atccgcgggt
    19081 ctaaatcaga tgacgaagca aaagcgacga tgatcgaacg gttcgattta tccgatcgcc
    19141 agtctcaagc tattttagat atgcgtttac gccgattgac cggtttggaa cgggaaaaaa
    19201 tcgaaaatga ataccaagaa ctcttaaaac tgattgaaga tttaactgac attctggcaa
    19261 gaccagaacg tgttacagaa atcatcaaga atgaattagc agacttgaat caacgatttg
    19321 gtgataaacg acgcacagaa ttactagtcg gtgaggtctt aagtctggaa gatgaagatt
    19381 taatcgaaga agaagaaatc gttattacat tgacaaacaa tggttacatc aaacgtttgg
    19441 caaataatga attcagagct caacgaaggg gtggacgtgg tgtccaagga atgggtatcc
    19501 acaatgacga cttcgtcaaa aatctggttt cttgttctac ccatgataca ttgttattct
    19561 ttacgaataa cggaaaagtg tatcgggcaa aaggatacga aattccagaa tacgggcgca
    19621 cagccaaagg aataccaatt atcaatttac taggtatcga ttcgagtgaa agcatccagg
    19681 caatcattgc ggtgacggga gaagcagaag aaggccacta tctcttcttt acgacgcgtc
    19741 aaggtacagt aaaacgtact tctgtcactg cttttgctaa catccgaagt aatggtctga
    19801 tcgcaattgg tttgaaagaa gacgatgagc tcgtcaatgt gctactgaca gatggtcaat
    19861 ctaatatcat tattggaaca cataacggct actctgtcac tttcaaagaa gaagcagtcc
    19921 gcgatatggg acgtacagct tccggggtta gaggaatacg cctgcgtgag gaagattatg
    19981 tcgttggggc agccttaatg aaggaagatc aagaagtatt agtgatcaca gaaaaaggtt
    20041 acggaaaacg gacaaaagta tcggaatatc cagtgaaagg ccgcggaggt aaagggatca
    20101 aaacggcgaa catcactgaa aaaaatggtc cactagctgg attgaccact gtttctggag
    20161 atgaagacat catgctgatt acagataaag gtgtcattat ccgtttcaac gtatcgactg
    20221 tttctcaaac tggacgttca acattaggcg tacgtttgat gaagatggaa gctgatacga
    20281 aagttgtcac gatggcagca gtcgaaccag aagctgcaga acaaacagtt gaagaacaag
    20341 gatcagaaga atagccatca agactaattg tgtaagctag caagaatgac cgattaaaaa
    20401 atctggttat tcctgctggc ttttttgatt agttgaattt ctcttaaaat attcgaaact
    20461 gttcattctg tattgctttt gaccaaaata gagtgtataa tcagagcttg tgagtagtct
    20521 aattaattag ataactctcc ttgctcttag gaacctaaga gccgaaagtc cacagggagg
    20581 tgaatatcaa tggaaaacac gaaatatgaa atcatgtaca ttattcgtcc aaacattgat
    20641 gaagaagcaa aaactgcttt aatcgaacgt ttcgatgcaa tcttgaaaga taacggagca
    20701 gaagttatcg aatcaaaaga ttgggaaaaa cgccgtttcg cttatgaaat gaacggttac
    20761 cgtgaaggta tctatcacat cgttaaagta tcttctccat catcagcaaa tgctgtcaat
    20821 gaatttgacc gtcttgctaa aatcaacgac gatatcattc gtcacatgat tgttaaagaa
    20881 gaagcttaat tgttgtttca tgtgaaacaa tagaaaacga tgagaggaga tgactatttt
    20941 gattaataat gttgtattag ttggacgtct gacgaaagat ccagatttgc gttacacagc
    21001 aagtggtacg gcagtcgcaa catttacttt ggctgtgaat cgtaatttta caaaccaaag
    21061 cggcaatcgc gaggctgatt ttatcaactg tgtgatttgg cgcaaatcag cagaaacttt
    21121 agcaaactat gctcgtaaag gaacactttt aggtgttact ggacgtatcc aaactcgttc
    21181 ttatgataat cagcaaggac aacgtgtata cgtgacagaa gtagtagctg acaacttcca
    21241 attgctggaa agtcgttctg catctgaaaa tcgccaacaa agcggcggat tccaaagttc
    21301 tggtcaatca gctggcggat ttggcggaaa caataactct aatcaaacat ctcaatcatc
    21361 caacggtatg cctgatttcg accgtgatac ttctgaccca ttcggctcaa gttcaacgat
    21421 cgacatttca gacgatgatt taccattcta aacgaatttg ataggaggga aatagaatgg
    21481 cacaacaaag aagaggcgga cgtaaacgtc gtaaagtaga ctacattgct gctaaccata
    21541 ttgaatatat cgattataaa gatatcgaat tactaaaaag atttatttca gaacgcggta
    21601 aaatcttgcc acgtcgtgta actggtacag gcgctaaaaa ccaacgtaaa attactgttg
    21661 caatcaaacg cgctcgtatc atgggcttgc tacctttcgt tagcgaagac taataaatcc
    21721 taggaattga atgactgatc cttttgatca gctattcaat tccttttttt ctgtaaaaat
    21781 aacctctatt cgtttagtcg ctcgaatgac gaaagttatg gtaaaatcaa attagtcata
    21841 tatgggagga aaattatgga aaataaaaac aatcatttat ccccgaaacc tctcttcctt
    21901 atcctgttta tattaatcga agtattggta gtcttaattt taccatttcg ttttgttagc
    21961 gcttcggtca ttctattgat gaatttatgg ggtgttcgct attggttgaa aatgaaacgt
    22021 tggcttattt taaataaacg tgaaaaaatc cagcaggctt cgactgtagc tgaaaccaat
    22081 ctagcttatg tctcagaagt catgcctgta ggagtgattg cttatcagcc atcaagccaa
    22141 aaaatcgaat ggctcaatcc attagcattg aaaatcaaag atcaaagtgg attaacagag
    22201 gaagaattgc tagatacttt ttttactgca aaagaaaaaa agaaaaacca agtctatcta
    22261 gcagataaaa catttcagtt tgatatggat acagtaaagg gtgttgttta ttttaatgat
    22321 attacccaaa accagttgtt aaaactccat cagacgaaca gtcagccggt gattggtatt
    22381 ttatctattg ataactatgc agatgccatt gataaattgg atgacaaaga gatttcttat
    22441 ttgaactctt tcgtcactac actagtttct gactggatga atgaatacca tgtctttttc
    22501 aaaagaatca acgcagaacg gtttttcttc gtagcacaga tgatcgatct gaaaaagatg
    22561 atggataaaa aatttgattt gctggatcgc tttcgagcag aagctgaaaa acagagctcg
    22621 cctatcaccc ttagtatggg gattgcttat ggctcaagaa ccctcgatga aatcggtgga
    22681 gaagctcaga ataatttaga tgttgcttta gtccgtggag gcgaccaagt tgtcgtgaaa
    22741 gaggtaaaag aaggagcaaa acctgtttat tatgggggga aatctgctaa gaatgtcaaa
    22801 cgcacgcgtg ttcgttctcg agcgatgagt accgctttgc gtcggatttt tgatgaaaca
    22861 ggcgatgtgt ttatcatggg gcacaagttt ccggatatgg atgctttagg ctctgctttt
    22921 ggtgtcagtt gtctggcacg cttcaaccag aaaaaagctt acatcgttat caacgagaac
    22981 gagatcattc cagatgtaga acggtgtctt gaagaaatcc ataagcatcc tgaattagaa
    23041 atgcaactaa tctctccaga acgtgccata gagctgaaaa aagaagagga cctcctcgtg
    23101 atggtggatt accataaacc ttctctatcg atctctcagg gcctatatga gcaatttgat
    23161 aagattgtga tcattgatca ccatcgtcgt ggggaagagt ttccaagtaa acctctgctg
    23221 acttatatcg aatcttcagc ttcttcagct tcagagctag tttctgagct gatccaatac
    23281 caaagcagca aaacaaatcg cttagatcgt tttgaggcca cattattgct tgcagggatg
    23341 gttgtagata caaatagttt caatgtccgt acaactgcac gaacttttga cgttgccagc
    23401 tatttaaggt cttgtggagc agacgcatcc ttagtacaat atttattgag ctcggattta
    23461 gcgacatact tggagatcag caatttgatt gctggaagcg actatgtgac ttctgatatt
    23521 gttgtggcgg cagggacaga cgaaaaagag tataatagtg ttatagcagc aaaaacagca
    23581 gatacattgc tttcgatgat caatatcaat gcagctttcg tcattacaaa gcggacagac
    23641 ggcttgattg ggatcagtgc cagaagtacc ggcacgatca acgtccaaat catcatggaa
    23701 tccttaggtg gcggaggaca cttcacaaac gcagctactc aactggaaaa tacgacagtt
    23761 gaggaaacaa aacagaagct atttgctgca atcaaagaaa atatgaatca aatatatgat
    23821 aaggagtaat ccaaatgaaa gtaatttttt tacaagatgt aaaaggaaaa ggtaaaaaag
    23881 gggacatcaa agaagttcca gcaggttatg cacaaaatta tctaatcaaa aataacttgg
    23941 caaaagaagc aacgaaaagc agtatcagtc agttagcagg acaaaagaaa gcacaagaaa
    24001 aacacgaagc tgaaatcttg gaagaagcta aaaagttaaa agaattcttg gaaaaagaag
    24061 atactgtagt cgaattgaag gcgaaagcag gggaagacag ccgtttgttt ggttcgatcc
    24121 cttcaaaaca aattgctgaa gggttaaaca aacaatacaa aatcaaatta gataaacgaa
    24181 aaatcgaatt agaaaatccg atccgttcac taggctacac aaacgtacct gttaagcttc
    24241 atcatgaggt gacggctacg atcaaagttc atgtcgtaca agaataacaa atcttgatag
    24301 cgatgttcag ctggtaagta taaccagaag gaaaaaagac aaaccgaaat ttatctgaga
    24361 aaacagctgc agcaatgaaa gcaaaagctg caaatcgatt tcagattgat ttcggtttct
    24421 tttttgggaa cagcaaaata gcataaagaa aacgagtata ataaaagaga actatttttt
    24481 tattacactt tcggtacgaa tgatgacttt tctctcattt tcttgtaaaa tgaagcaatt
    24541 gaagacaagg agcgatacgt atgaatgaag tgtggcagga tcgaatccca ccgcaaaata
    24601 ttgaagctga acaagcggtt ttaggctctg tttttcttga cgcagaaacg atcattgatg
    24661 ccatggagta catagagcca aaagactttt atcgtcgtgg ccatcaaatt atttttgaga
    24721 caatgatcga gttaaatgat cgtaatgaag cgattgacgt ggttactgtc aaagaccggc
    24781 tggaacaagc caatttgtta gaagatgccg gtggtttaag ttatctctca gacttggcat
    24841 tagcagttcc gacagcagca aatatcgtct actatgcgaa aatcgttgaa gaaaaatcat
    24901 tgctgcgtac attgatccag acagcaacag gaatcgttac gaaaggattc gagcaaggag
    24961 aagaagttca aacaatttta gatgatgccg aacgcagtat cttggaggtt tcggaaaaaa
    25021 gaaatcgaag cggcttttta tctattgctg aagtactgaa ctccacgatt gaaaatatcg
    25081 atcgactagc tcaaaacaat gaagaaatca caggtctacc aactggctat caaggtctgg
    25141 ataaaatgac agcaggtttc cagcccgaag aactaatcat tcttgctgca cgtccagcgg
    25201 taggtaaaac agcgtttgca ttgaatattg ctcaaaatgt aggaacaaaa acagatcgtt
    25261 ctgttgcgat atttagtttg gaaatggggg cagaatctct tgtaaatcgg atgctttgtg
    25321 cagaaggttc gattgaggcc agccatttac gtacaggtca gctttcggaa gaagaatggt
    25381 cgaatctgat cgttgcgatg gggagtcttt ctcgagcaaa tatttttatc gatgatacac
    25441 cagggatcaa aatctctgaa atccgtgcga aatgtcggaa actagcgcaa gaaaaaggca
    25501 atctaggctt gatattgatc gattatttgc agttgattga aggtaacggt aaagagaacc
    25561 gtcaacaaga agtatcagat atctctcgcc aactgaaaaa actggcgaaa gagctgaaag
    25621 tacctgtcat tgctctttca cagttatctc gtggagtaga acagcgacaa gacaaacgac
    25681 cagtgctaag tgatattcgt gaatccggat caattgaaca agatgcggac atcgtagcct
    25741 tcttgtatcg agatgattat tatgaaagag aaggcggaga tgacgatgat ggaaacgagc
    25801 caaaaaatga caacgtcatc gaagtcatca tcgagaagaa ccgaagcggt gcacgaggaa
    25861 cagttgaact actgtttatc aaagaataca ataagttttc gtcgctttcg ccaagagaag
    25921 aattttaaag aagaggttgt gagccttgag caatcccgag agctctcgta agaagatgca
    25981 aggagtaacc gcaggaacga ctagagtact taatttaaac agatctccaa agggattgtg
    26041 acaaaagtct tgtcacagtc cctttttccg aataaacggt gttcaatcag ctgacccttg
    26101 gtattagctc agcaaacgtc aaaatatgag gaactatttt gacgtttgtt tctctaatac
    26161 tcaggtcaaa acgctgatct tacgacctct tttccttaaa cggtgttctc ctgccaactc
    26221 ctcggtcaca agtcacaata tcatcaaaca tgagaagcag ttttttgaaa ttgctccttg
    26281 tgactcagag ttaaacggca ggctcacaac ctctttctta aacggtgttc aaaagcttgc
    26341 atctgcggta atacgcccaa tcaaacgaaa aatatgag
//
LOCUS       DACEKH010000030        26351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEKH010000030 DACEKH010000000
VERSION     DACEKH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.030"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(97..1227)
                     /locus_tag="IW662_001184"
     CDS             complement(97..1227)
                     /locus_tag="IW662_001184"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ0866020.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1876..3078)
                     /locus_tag="IW662_001185"
     CDS             complement(1876..3078)
                     /locus_tag="IW662_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0866021.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3138..3341)
                     /locus_tag="IW662_001186"
     CDS             complement(3138..3341)
                     /locus_tag="IW662_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0866022.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3483..4715)
                     /locus_tag="IW662_001187"
                     /pseudo
     CDS             complement(3483..4715)
                     /locus_tag="IW662_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4878..5690
                     /locus_tag="IW662_001188"
     CDS             4878..5690
                     /locus_tag="IW662_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0866023.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6714..6866)
                     /locus_tag="IW662_001189"
     CDS             complement(6714..6866)
                     /locus_tag="IW662_001189"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866024.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7057..7935)
                     /locus_tag="IW662_001190"
     CDS             complement(7057..7935)
                     /locus_tag="IW662_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0866025.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7974..9092)
                     /locus_tag="IW662_001191"
     CDS             complement(7974..9092)
                     /locus_tag="IW662_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0866026.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9089..11188)
                     /locus_tag="IW662_001192"
     CDS             complement(9089..11188)
                     /locus_tag="IW662_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ0866027.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11239..12060)
                     /locus_tag="IW662_001193"
     CDS             complement(11239..12060)
                     /locus_tag="IW662_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0866028.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12057..12866)
                     /locus_tag="IW662_001194"
     CDS             complement(12057..12866)
                     /locus_tag="IW662_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0866029.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12887..13381)
                     /locus_tag="IW662_001195"
     CDS             complement(12887..13381)
                     /locus_tag="IW662_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0866030.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13393..13881)
                     /locus_tag="IW662_001196"
     CDS             complement(13393..13881)
                     /locus_tag="IW662_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0866031.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13958..16654)
                     /locus_tag="IW662_001197"
     CDS             complement(13958..16654)
                     /locus_tag="IW662_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0866032.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17082..18155)
                     /locus_tag="IW662_001198"
     CDS             complement(17082..18155)
                     /locus_tag="IW662_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0866033.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18152..18979)
                     /locus_tag="IW662_001199"
     CDS             complement(18152..18979)
                     /locus_tag="IW662_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0866034.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18980..19786)
                     /locus_tag="IW662_001200"
     CDS             complement(18980..19786)
                     /locus_tag="IW662_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0866035.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19798..20883)
                     /locus_tag="IW662_001201"
     CDS             complement(19798..20883)
                     /locus_tag="IW662_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866036.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20900..21442)
                     /locus_tag="IW662_001202"
     CDS             complement(20900..21442)
                     /locus_tag="IW662_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0866037.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21700..22689
                     /locus_tag="IW662_001203"
     CDS             21700..22689
                     /locus_tag="IW662_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0866038.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22686..23423
                     /locus_tag="IW662_001204"
     CDS             22686..23423
                     /locus_tag="IW662_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ0866039.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23420..23971
                     /gene="coaC"
                     /locus_tag="IW662_001205"
     CDS             23420..23971
                     /gene="coaC"
                     /locus_tag="IW662_001205"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ0866040.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23987..24619
                     /locus_tag="IW662_001206"
     CDS             23987..24619
                     /locus_tag="IW662_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0866041.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24704..25351
                     /locus_tag="IW662_001207"
     CDS             24704..25351
                     /locus_tag="IW662_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0866042.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25356..26339
                     /locus_tag="IW662_001208"
     CDS             25356..26339
                     /locus_tag="IW662_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ0866043.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8060 a         5570 c         4465 g         8256 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt gccaagcctc acatttttat agccaaactt cttttgcgac
      121 ttgctgcaat aattggattt tcttccattg ttcttcttct gttaaatggt ttccttcttc
      181 tgttgaagca aacccacatt gaggactgat acttagattt tccaatggta caaattgttg
      241 tgcttctttt attcgttctt ttattttctc cgtatcttcc aaatctggaa acttagaagt
      301 aactaatcct aaaacaactt tactttcttt gttactccaa tgtcttaaag gagtaaaatc
      361 tcctgctcgg tcagaatcgt attccaaaaa gaaaccatca taatttgttg aaaacaactc
      421 ttctgcaatg ggttcatatc caccggaaaa cagccaagaa gaagcgtgat tcccgcggca
      481 gatatgtgtt gtgatcgtca aatcttctgg tttgtcttcc agtgcgttat tgataatctc
      541 tgtacaaata tgggccattt ctttcggatc aattcctttt tgaagaagtg cttctctttt
      601 agtttgatca gctagaaacg cccaatttgt atcatcgaat tgcaggtaac ggcaaccttt
      661 ttcataaaaa gcatgaatcg ctgctttgta agtagaggac aagtcctcat aaaaatcaga
      721 tagatcagga taaatctcct tgatttttga agaaccatca ttagatagga tctcttgtcg
      781 taaaatcatc gtgggactag ggatcgttgc ttttgcaaca aaagtatcat ctgtttccac
      841 tacttctttc agaaaagcaa aatcttttaa aaatgggtga tttggattga aagataattt
      901 atctactacc cgaatattat aagctggtgc tgcttttccg tggaatgttt gattatatcc
      961 agtttctgga acatagcctt cgataccatt tagttcttct aaaaaatcaa gatgccaaaa
     1021 tccacgccga aattcaccat ctgtaattcc tttgatgccg actgcttttt ggtcttccac
     1081 taattttttt atttcctggt cttcgattgc acgaagttct tcttttgaaa gtaagccttc
     1141 ttgaactttt tttcttgctt ctttgatcga ttgcgggcgt aagaaacttc cgacatgatc
     1201 tactgtaaat ggtctatttt tgctcatttc actcactctt ttctttgttt tttgtaaaaa
     1261 aaaatccttt tactagcaaa tactagtaaa aggacgatta tactcgtggt accacctttt
     1321 ttcagaaaaa acattctttc tctcagcctg acaataaaat cagtggtctt ggtaacgaaa
     1381 gactgttcgt ttttgcctac ctttcgacaa aaaatcctct aaggccattt tcgacagtca
     1441 acttctagct ttttacacca accaaagcct ctcttgaacc attttctgtc tactttcctt
     1501 ctcatcggat actattagaa taaaattaga atagcatgtt tgaaagaaaa gtcaataggg
     1561 aataagaaca aacaaaaact aatttgttat tcttagtaat ttgtttgtgg aatacaataa
     1621 agcttttcta atatgataat tgttcagtcg tcctttcatc ttagatcact tccttaaaat
     1681 gatcataccc gtttcaacca cataaaaaag gacttaattt tgaaaaattt cccatcaaaa
     1741 ctaagtcctt tttattatag ttatgcaaat ttgcttattt tctcgctatt tccgatacat
     1801 cttgaacagg agcaattcga ctgaaaacac tggtgcaaaa gcatttattt gtcttatggt
     1861 agaaccgtgg aaaactcaga ctggtaataa attgtcattt tttgaaagaa tagcgccctc
     1921 ttgaacatta tcaatcgcac ttaccacttg ttgatctgac tttgcatata agtgaccgta
     1981 agttcctaaa gtgatttgaa tattcgcatg gcccaatcgt tcttgaatag atagcggttg
     2041 aatattctgc tcaattagaa atgcaacatg agaatgtcgt agcgcgtgaa gcttaatcgg
     2101 tttgacaccc gcttttttag cagcttcttt taaccagttc gcaaaagaat aaggtggcgt
     2161 aaatgttccg tccaactgag caacatattc aaaatttcca atacgttttt gcaagtcata
     2221 ccactgtctg agttttaagg aaagcttctt ccccaaccca atcgttcgat aagcacattt
     2281 agttttggga gtcgaaaagt accaatcatc ttttgtatta taaatcaatg tcttctcaat
     2341 cgttatcgtg tttttctcaa aatctacatt cgaccaagac aaagcttcaa gttctccaat
     2401 ccgaatgcca gtataaaaga gcatttcata cacaatcttc cgatggtggg tttgaatctt
     2461 atcatccttc aattcattca tcactttttg aaactcactg tgttcccaaa actcaacttt
     2521 cttattctca gaaggaagcg aagaaacatc ttcaacggga tttttagcaa tcaccccctc
     2581 tttaacagcc cgatccaata caatttttag gtgccctcga atccgattga tataagattt
     2641 tgataacgtt tcgccattaa cttttttccg tgaattacgt gactctgtct ggcttagttc
     2701 aaataaccaa tcctcaatat cgtgacttgt aatatctcga acacgaaaat gatagaaaaa
     2761 ctgcatacgc ttttcgaaaa tgaattgcgc attctcatat gtttgacttt tgacctgtct
     2821 ctcgtaccat tttacaaacc tatccttgta aaactcttcg aaagtcattt gattggtaga
     2881 acacagttga ttaggaattg tcttgttaaa atcatgtgtt tccatgtact tgtcataagc
     2941 aattttggca tctttttgtg aagcataacc tcgtttagtc ttctgaacct ttcttccatt
     3001 ttcatctata cctagatacg ctcggaagaa ccattttttt gtctttttat cttggtaaat
     3061 atttttaatt tttggcatcg ttccattcct ccctgtactt gagaaaagct actttaatag
     3121 taccgcgctt gtactgttta ttccgcaact tgaatgtgaa aaagatgttc aattactcgg
     3181 gatggcacca caccaacttg gcgattatta taaaaatcaa taccttcaat ccttgcaaga
     3241 tatgccttgc tctctttaat cattcgtgca gcttgactag gtttaaagcc catctctatc
     3301 aaaattgcac gatttaccat gaaatcacat tttgtttcca ttctctcacc tcctgattct
     3361 ttactccttt taaaataatc ctctttataa tctattgttt ctcattcgat ggttgtgtta
     3421 tactcgtctt gtcgaggcgg agtacgttgc acaacttgtt gtgtaagcag tactttgctt
     3481 ttttagtagc ttgttttctc atttgatata ctttgtaatt ttcgttcata atactcaacc
     3541 tcctcctgat aatactccgg gtctgccttc atattttgga gaagtttgat ctgtcttgga
     3601 ttcatttctc cttgttcaat aatcaaaggc aacaaggaag aatgaaacgt ttgatcgacc
     3661 tgtgcaataa aaactaatga tggtgaaaca ccatgaatta accaactcat agttttctca
     3721 aaactttgag gaattgtatc taaagaaatg gtgattgcgc cataattgct aataaaggct
     3781 gcccatttgg catcaacttt tgcacctaga tcatttggat cacggtcata aaaacaaatt
     3841 gctccattaa gtaactcaaa gaacagtttg gttaaatcat gggtttttga aataatcttt
     3901 gccaagcttt gcgctttctc ttttcgatac cgtagttcaa agcgattctt tacttttgcc
     3961 tcttctaaag gaattcctcg tcgcttccgt tgttcatggt ccttctgata aaagcaaaaa
     4021 cgacactgac tcttctttga gcctaaatac aaggtctcac catttgattc attgtttatg
     4081 atatccgtac cgccatgaga ttcactcact tgaaaagtgg tccacacatg gcctagtatt
     4141 accttatgct taagttctgg aatgctgaga ctccccacaa aatcatctaa agtaaaatca
     4201 atccgtgtaa agttcccctg ataatctaat accttacgaa aaaactgttg ccaatttgtt
     4261 tttcgtgttt ttagccatct tgctaagtgt ttacagcctt gacctgaaaa ctccatcaca
     4321 gttcctttaa ccgcatcgtc aatggaatac cgaatcgtga ttacttgatt ccacacgtat
     4381 tggccaatat agccctttgg cgcataatcc agttcctcaa aacgttcaat gggcatttca
     4441 ataatctctt caattagctt taaatgttga ttcgagaaaa agtgaatggt tagataatca
     4501 atcaaaacac ggttaactgg aacgacctct tcttgcaaac tatccaacac aagattcaat
     4561 ttttcttcac ttaattgtct cgttcctttt tctactttcg ataataacta tctcgaaatc
     4621 cccacttttt ctgctaaata tcgttgtgtc cacccccttt cttctcgaga aactcgaatg
     4681 gcttcaccga gatcatgttg attaacttgg ttcatcgaca aacctccttc tgctatttac
     4741 ttgtcaaaga acctattgaa cttattttaa gtacattttt attgtacgcc tgtatttatt
     4801 ttaagtcaat atgttttgac tttttttaag tacacacgta tactaaattt aagaacttat
     4861 cctgaggagg agttttcatg gagatagaca agcaagcagt cggcaatcga attcggcaga
     4921 ttcgccaaga attaaagttg agtatggaga aatttggcaa actaattggt gatttaccgc
     4981 gaagtacagt caataactgg gaacgtggga tcaaccttcc aaaaaccgaa acccttcacc
     5041 aaatcgcaga gataggtcat gtcaccaatg aatatctttt gtatggtgat caagagaatc
     5101 aatacatttt agaaatgctc caaaaaaagg caggtaagct tgatcctaag attgaaggtc
     5161 tcatagtaga cgaaatcaaa cacgctgacc ttgttagtga aaaagaaatg gatcgaatga
     5221 ttgaattttt tattacaaat ctcattccac caactgaaca agaccagttt actttccaat
     5281 gtatcgatga aaaaaagcag ctttatcttg gttcaactaa ttttggtaag gaagcccagc
     5341 tctatttgca acatgataat caaaatcata ttctgcacat gatgcccttt accttctcca
     5401 actttagtgt ggatcgcata ctcgtctatt tagcaaatca aaaatcctat tcctattttg
     5461 ggaaacattt acccaaggaa ctaggtgaga aagccatcct tctatactca attaatcaat
     5521 tgaccagcga tgtgcgaatt gccccgttgg tctattcaga agaaaccgct tcttatcagt
     5581 acacagagga caatcaatat ctactagaac agcaatatct ttatctacca ttcgtgatgg
     5641 aagtcgagaa agaacgcctg ttaaatgcag cgtatccatc atcaaaatga ggtgacaaaa
     5701 ttgataagca cgtgacaaag aaaatactgc taggacactc cccaatgggt agttgtcaca
     5761 gtttcctgtt ctttgttagc cccctgttag aaccgggggc tttattcctg cagtccggct
     5821 acgccttcct gcaatagaca aaactccaga tcgggtaatt cgcttgtggc gaaacccgac
     5881 ctggagtttg tctctgtatt ttgaacccta aaaattaatc aaacagactg atattctcat
     5941 aatcagcgcg aaataagcca ttaagtacag ttttcctacc tatttttgat aacccgctgg
     6001 ttagttaata ttccttttcc gagagagata aatgattggt taaatctata tacatctcaa
     6061 cctttaaaaa tgttttcgaa atacttcatc agtacatgtc atacaaatta ccagtgcatt
     6121 ctcaaattct agttctttaa ttgatttcag ttgaggagca gttacttttg gcggaaattt
     6181 ccatttttgt ccaaacctct tcccccgatg aaatttcttt gttgcaatta ttgcaatgaa
     6241 aaatttttgt ttcaaaaata tacataatta cttcctccaa taaaaactat ctacctctca
     6301 aattattttt ctctaataag aaaaacagaa aaggtaataa gatacaacag tgatgattcc
     6361 taataaagaa aagactacaa taatattttt ctaacttatc gccttcaaag aaatagtttc
     6421 ttatttttca ctaaaataaa aaatttattg caaaaaaaat agaagtgaca aaattatcaa
     6481 tcatttcaag gtaatataac tacctgagtt tattatcgat taaaatgcta actacatttg
     6541 caagagaagc gagagttaaa ggtaaatata gaatttctac ttttttagca atagaaatag
     6601 gaataaaata tttaaaatgt atagaaactt tctttttttt tcttgctatc tttttataat
     6661 cacaactctc tataatagaa ttacgattta ttttatacgg ctgaaaaaat ctatcaccaa
     6721 aaaaattcaa acattaagcc aaggattatg aagtcaacat ttcgaaaaat gaacatacat
     6781 tggtaagcaa ctataactat taccacagac ataaagtaac acaatatatt actatttaag
     6841 tgtacgccac cagtgaatat cctcaaggta tacagcgcac aacaaaaaga aaaaaggaaa
     6901 cgcagaagca tttcctttca gtgatttaat gaaaacaaca cgatttgact tagagccttt
     6961 ttatttgaat tcattttcgc tttatccaac ttcatttgat catttgatta ctcggttaaa
     7021 tacttttgac atgagtatca atgaaacatt cattaattat ggttgcttat ttcttaccgc
     7081 ttttctggcc aatgctaatc cgccgattat tccggcatta tcacctaatt cgggcgtaat
     7141 aatgtagcta tcaattggtg gtatttcaac atatccattt ataagctcaa caaatttatc
     7201 ctgaactttt ttcttcatgt gttcttgttt cattacacca ccgccaaaaa taattctatc
     7261 tggcgaaaac atcagtgttg tattgtatgc acattgggca aggtaaaagg cttcaatctg
     7321 ccaaaacgaa tcgtcagcca ataaattctg gccttttaca cccaaccgtt tttcaatcgc
     7381 tggtcctgct gccatcccct ctaaacaatc ttggtggaag ggacagtttc ctgaaaaatt
     7441 gtcctttgga tgtcgcttga tcaacatatg ccccatttcc ggatgactga acccttccac
     7501 gaaagtccca ttgaacaagg caccacctcc gatacctgtt cccacagtga agtatgcaac
     7561 acctgctaac cctttgccag aaccagcaac atattcacca tagcaagccg cattgacatc
     7621 tgttgtccat tcaatcggaa tattgaaata ttgctttagc gttcctacaa aattgaaatt
     7681 ttgccaagca agttttggtg ttgatgtgat atgaccgaaa ctcgttgatt tgcattgaat
     7741 atcaattgga ccaaagcagc cgaccccgat gcctgccaat tgttccttaa aaggtttaaa
     7801 aaaatcaatg acctgcttca ttgtttcaac tggagttgtt gtgggaaaac tgactctttc
     7861 caaaatcgtt aattcctcag taccgactgc acagacaaat ttagtccctc cagcttcaat
     7921 tgccccatat atcatcatag gttcctccct tcgaatatgt gaaaaacact tcattaacgc
     7981 ggcaagcgtt ctcgatcagt gaaatgacgt ttagtatttc ctgaagcttc tgttgaatac
     8041 cagtaagcga cactagcaac atcgtcttgc cgctcaaata agcgaacatc atcgttgccg
     8101 atttgttgta aagtgaccga taaatcttcg gtaaaacaga tggggtcttg aagatgccaa
     8161 cgataaagac catgtcgtgg cattgcgtcg tcaccaaagg catgaacggc attcaatttt
     8221 cccgtttcaa aacgatctct tgtggcatct ctatttgatt ggtaaggata ccctaaaaag
     8281 gctgttgaat agtttttcgc atgtggacgt ccgaactcat cgtactcatg aaaggcccac
     8341 gccccgccaa aataatcttc tgaacctgtt gaggttacgg tcggatactc actatcccca
     8401 tcaatgtaga atttaaactc gccttcgccc caccaataac gttctaggca ggtaagcgca
     8461 aggtaagtac ccacgtaata gccttgaccc tttaatcgat caattaactt ataatcctcc
     8521 tgtaatttgg taatgcgttc ccgatgccaa tttgcgtgaa aatataggct attttcttcc
     8581 aattcgtcca ccaaagcgta atttatcgta taaaaaaatg aggacatatc cttcggatgt
     8641 aaattctcga tcgtaatttt agcggatgtt tggaaaggca tttcgaaata ggaattaaag
     8701 ccccctgtag gattaaccac tatcggcaac gagttcacct cgcaacgctc tccaaaccca
     8761 ttacaaaaga aatcccctaa aggactttca acagcagggc tatccgaccc atcccaataa
     8821 attcttaaga ttaaatcacg taaaacaaaa ctgccaaatt ctgttcgatc acgaatcgtc
     8881 atccacatat gacgaattat tcccgttcca gtaatatccg caatcacagt ttcttcacca
     8941 gaagtcagcg gaattgcgcc acgacctttg cggcttggtc ctagctcgct tggcaacatc
     9001 gcagctttac ctttctcgcc tgttggattt tcgggagtga ttgctcgact ctggattttt
     9061 ttaaattggg taatattttt tagtatactc atctttttct cctttgaatg tgaatcgttt
     9121 ttcccttttc tacgtgaaga atttttttcg tatcacttat tttcaatggc ttttctgaca
     9181 aatttgaaat actcaactcg gattgcgtac attcaatcga aatgaaggct tgaccgaact
     9241 tcaaattctt ccaagaaaac gttaaattat caacaggatc aatttgcaat actccttttt
     9301 tgaatttcaa tccgaaatat tgactgatca ttaggctgac aaaaagaccc gatgcccagc
     9361 cgcatttgcc gcagccaaag tctcgcacga cttctccttg ttgagcctct aagcgatacg
     9421 gccaccacca ccacgaaccg tctaaatctg ccaaacaaat tagttcttct aatcgggtta
     9481 cgaaagcttg agggttgtgc agctcgctca ttaaaacagt gataaagcca ggaaaagtgg
     9541 cgccggattc taatccccaa gtgatgcctt tgatttcctc gctataggtt ggattgaaag
     9601 aactagcaga aaaggccatg gtgttctgat ataaatgatc tttattatct aaaaattgat
     9661 aaaaaggcat caacgtagtg tctgattctt ctccatcatg catcaataaa ttaatttttc
     9721 catccacaat cacatcactt aaaaagatta acccttgctc aagaatcggt ttttgatagt
     9781 gcttcactga ataggttgtt ttctcctcat caccgattcc ctccaaaaat tgctcaccaa
     9841 aaggaccatc gatagtcatt tgtttacaaa ttgattcctt tagtttacag gcaatgtttt
     9901 tgtatcgctt gctaagactc agttgttcta gggcttcaaa aatttctgca agtccaatac
     9961 aggccttcca aagacaaaga ttgctgccgg tatgatactt tccaagtgca aaggcgtctg
    10021 ataaccaaac agatggaaaa agcattggtt cccctgaccg acgttgagct aatatgtcct
    10081 caataattct tgaagcattc agtagaacct gcggacaccg ctcaaagaat tttgtatccc
    10141 cagtatgttc aaaataaaga ctagccagta acgcactgga taacgaatta ctcagagaat
    10201 gattgatacc tcctgtaaat tttgaaccag gaaacttaac gccatattga tcaaaccata
    10261 gaaccgtttt ttgacataac tccggttcaa acatggtgaa tggcaatgat gcgtaataca
    10321 tatccttatt ccaaattcga ttagtagcag gatagctgcc ccaattgctg ccgacgacct
    10381 gtccctgtcg attcataccg aaactagaaa agctttgata aagtgcacga cgatagagat
    10441 ggacaactct atcgtttggc attgacagct gcccaaatag ctgagcaaaa tagttcaatg
    10501 tttccttcag ccatttttcg atatcagatt cctgaaaagt cattacttct tcaaaagcat
    10561 ttgggtccac aaaagcaatg gcgaagcttg ccacttcctg ttgtgcgatc gtgtccttat
    10621 ttcctttttg agaaatcaac acattttgct gatccgaata cttctcttga tacgaaggca
    10681 catctttcac tgaaacttga aggctttcgt gtgtttcatt cacaatgtaa acctgttgaa
    10741 caagcatact taaacgctta ttagccagta tgggagcaaa ggatactaca atgaccttaa
    10801 acgtcgaata atgatgaata tatctaggca aacagtcttg aatcacatcg acttcaatac
    10861 gatgatccga ttctgacaaa tggtagacaa tcccgtttac ttcaaccttt aaggataacg
    10921 aattggtttg agagagctgt ttattgaccc aaacacctgg tttttcctca gttagtaaat
    10981 tttctcgata aaaggtcaac gtttgaatcg tcccgcaaac atcaaggttt gctaatgtct
    11041 tttgattact tacatcgaag gtcaatccct ttacatcagg aatattccga tagtagctgt
    11101 attcgtttga agtagtcaca tttccgtcgt ttaactcagc attaaaaaat gggtgtttgg
    11161 gtgaataaat tacttcatta atagtcatct tttccctcct tgctaaaaaa actgcagcct
    11221 agtagctgca gcttcctact atgcgattcc aagtaatgac aataaaattc ccaagacgac
    11281 cattccaaaa ataattttga tgacgccgac ttttttatct agtaatttga aacagctcaa
    11341 tgttaccaat aacggtacaa ttcctttaaa aatactatcg aggatttctt ggacattcat
    11401 tagttcttcg cccttcattg aaaagctcaa aacggtattg aatttgacca tactgatcgt
    11461 cattgcacct accatgatca gacccaagat actagcagct tttgtcagaa tttcaattaa
    11521 cccgttcgca tacagctttt caatatattt agagcctaat gaatagccta aaacgccgcc
    11581 atagtatcgc atcaagtaat taggtatatt gaacataact aaaaagataa ttggtgccaa
    11641 aatatttccg gcattggcta agttgatcgc tagtcccgcg gtaacgacac gccaaactcc
    11701 ccagaacaat gaatcgccaa ttccagccaa tggtcccatc aaactcgttt tgatcgcgtt
    11761 gatactttcc acgtcaaaac cagctttttc agaattttcc ttctccatcg atgccgcaat
    11821 ccccatgaca aaggacgcgc aaggaatggt cgtcgaaaaa tactgtgtat gacgttccag
    11881 cgccgcagct cgatcttctt cacttttgta gaaatgatta atgaagggca tcatagaata
    11941 ggcgaaacca ccgccgccca tcgtcaccgg attcaccgaa gcatacatag tcatcgagcg
    12001 ccaaaacatc ttccgaacca attttttctc atctttagaa attccatcag ctaatttcat
    12061 tgaaagaaat cctcctcttc ttggtcaagc gttgcattta cagcagcatc gctttgaact
    12121 gcatgaacag atatattttt tagttgtaga tctctttggg ctataacaat tgcaataatc
    12181 gttccaactg cggcaattgc aatcaatggt aactcgagat acgccataac aacggcccca
    12241 aagaaaaacc acacagcaat atttttactc caaagcatcc gtaacagcat cgccatccca
    12301 actgccggca gcagatttcc agcgactgtt aatccgttca tgatgacctc tggaatatta
    12361 tccaagacgc ttttgacaac atctgctcca aataaaacgg caaagaatgg aataagagca
    12421 taaagcgcat agtggacaaa ccacataaag aagtgcagat tgatgattcc tttttgatca
    12481 ccagattttg caaatttgaa aaatttagca gccatcggtc cacatacaaa agaaaaaatc
    12541 atattcatta actgtaatcc taatactgct agtggaaaga caatgggaat aatcgcatcc
    12601 acgtttcctc ccattttgat tgcgaacgct gtagccagcc cagcagccaa agaaggctcc
    12661 gccgtactag agattccgat attgacgaca cccataaaga tggcttctag tgacgccccg
    12721 acaatcaacc ccgtctgcaa atcccccatc aacgctcctg ctaacggagc aacaacaatc
    12781 ggacgactca gcatggacat cccgaacatt tcatgtccag caactccaat aaaaacggcc
    12841 aaagcgacta caaatgcatc atacataatt tttctccttc ctttttctaa aaatcactca
    12901 aaatagagat agctgaacgt ttttccatcg ccggtgtagg ctgaaaaacg gtatctggat
    12961 aaagttcaac taattctttt aagtcttgaa tttctgacgt cgttaacatc acttcctttg
    13021 ataaggtttt cttatcttcc gatggcggtg tcattttccc agcattcccc acgttcatta
    13081 attcgatttc cgttgggaaa gctttcgcaa cttttaccgc atctttgaca ttagggacta
    13141 aaaccatcaa gcgcatgtcc ttcgcccgcg gatcttttaa caaatgaatc gcgtcatcaa
    13201 ctgttttaat aatgactttg acattcgatg gtgcagccat cttcaatgtc atcttttgaa
    13261 tatcgttggt agccgcctca tcgcttgcta ctactaaccc ggttaaattt agactctttg
    13321 tccaagtcat cgctacttgt ccatgaatta atcgttcatc aattcgcact gctttaatca
    13381 tttttcttcc tcctaaaaaa aatcttcctc ttcatttact acatgtacct catcaagaat
    13441 ggtcatcttt aattcctgtt ggcatttatt taccagttcc tgaatatcct cctttgttaa
    13501 ttccttggtt gccaatacta tttcgagcag aattggcaga ttaaagccag acaaaatagt
    13561 tacagcaatc tctttctcca aaaaggtttt ggtaatcttc tgattgacac ttccgccaaa
    13621 taaatcggta aaaacaaaaa tctgatcctg ctcgttcacc tggtttacaa atacattcaa
    13681 ctcctgttca aaatcacact catccacata agcattaatc accgtaatct cttgaacctt
    13741 ccccgtcagt aattgaatac tgctaagaaa gcctgaagct aagtgaccgt gagtcgctat
    13801 tagaattttc ttcatatggt taattccttt ctttctgttc acttttatat aagcaattcc
    13861 tgtgccaact tttaaaaaca ttggtttaat cacgttaatt cacattaaaa aaagtgtcta
    13921 acctaaaaaa aacagattag acactttttt agacacttta tttttgaata tacggtccta
    13981 ataattcata gagcagcgaa agctcataag cattgacttt aaaattatat ttcatctcca
    14041 ttgattgaaa aatacttcta gaaacataat aaaattcatc ttcatctggt ttcaaatcat
    14101 aattcggttc ttcagatttt tctgtccgag ccaagaataa acgttccatc atcaatgccg
    14161 tatgcatata gagatttagt tttacacggc catttaaagt gaaatgatag taattttcat
    14221 atttagaaat gactgtttcc acttcactca tcacgatcgt tgggttcaga aagttcaaac
    14281 ggtccgcaat tccttccaac gaaaagaatt ttactagctc ctgaatcaag cggttaaatt
    14341 ctgccggctt catttcagga ctcaaaatat gctccaaatt ggctgctcca tcaccgtcta
    14401 agatgtcata aacattaata ttaggaattg aaaaggaacg tggaagatcc gaagtcgtga
    14461 tgaccaattt tgtcttgtca aaataagttt catcattttg gtcgattaat tcacgtagtt
    14521 ctctgtagtc cttcgtgaaa acttcgatgg attcatccga taaatgatga cgaaaaacat
    14581 ctttcaattt gtccgaaatc cccaaacccg acatacagga aatgataatg ttgctacttt
    14641 gagaaaagcc ttcaaagtat tgaacattga ttgaatactt gtctgctgca tcctccgcaa
    14701 tctgtttaaa tggcgcattc atcgtcatct tcaagccaat atcaagtgca atggaagtcg
    14761 tcaaattatt cagaagaagt aactcccctt ttaaatcgtt cttgatctgt gtgtaaattt
    14821 gatttaaaga gcccatatct acgatcagca ctagactatc tgtattcact tgccgtttca
    14881 cgaaattcct tgtcgattcg acaatctctg acacattact ctctactggc atatccaacg
    14941 cttcaaaaac ataattgccg cataattgat tcactactgc ttgaatgctg ctagccgtac
    15001 tatctccgtg agcaagcaac aggcccttca gctcgatgga ttcatctaca taatcagata
    15061 aagtcacgac aataagcagt cttagaaagt tctcgtcctt ttctgagagc ggtggtaaat
    15121 gctccaagaa ctgattacat aagtaagtcg tccgaggtag atttgcagct aatgtacgat
    15181 ttatcccggt taagtctgtc ttatagcttg gggaaatgac ataggatctg ctaataattt
    15241 ctgcaacagg tccactcgtc gtcaatccgt acgtttttcc taaaacggat acccagacct
    15301 ttctaaacat ctgaatcatc aattttctac tcattgataa aaaagaatcc ttatcaatgt
    15361 gatgcaccaa atgctgtact tcattcttta tcgaagaaaa attttcattg aaattacctt
    15421 catttatcag ctgagtaatt ttttccaaaa tcgaatcata atcaaacggt ttgattcgat
    15481 ccgtttttat cttttgattt ttctttatca ataaactagc aatcggcaag ctttctactt
    15541 cggattccag catatttaac tgaatcaaga tctcattttt cccaatcgtt aatggttctt
    15601 cttgtgttcg actatacaca tccgcacaac tgattttaat gatattcttc agcttaccaa
    15661 tattcccact aaagggatga gataacatta aagacaaggc ttctcttgaa acaataatat
    15721 ctttttgtaa aacatccgct tcattttgaa aaaataattg aatcagctct aatcgttctc
    15781 gaatggggcg atcttcaaaa gttggcaaca taacactaat ttgaatacgc cgatcaaagg
    15841 tgtctaataa ataattttca cctttttccg tcgtggcgaa aataaaacga accttagcag
    15901 aatgccaatt tacattttca ccgactggac gatagtttcc agtatcaata aatagaaaga
    15961 gcttttcttg attttctttc gataggcgat ggacctcatc gagaaaaaga tagccgttgt
    16021 tggccgttgc caataaacca tcggtatcct tttcagcccc cgtaaaggcc cccttcttgt
    16081 atccaaacag tgttgccgaa agcagttcgg gattgtccgc ataatcggca cagtttaaga
    16141 tagcaaatgg tgcatctttt tcaataattt gctcatggat agcgtattca aaaatttttg
    16201 tggctaaaaa actttttcca acacccgtgg ctccatttat taatacactg agtccgttag
    16261 gcggatattt gacagcggct atacattttt gaatgacctc tctcagactt ccgtcatgac
    16321 caactacaga atgaaaggct tcgtttattt gctccgtatt atctacaacc gcatattcat
    16381 ctaaaggctt ccacaaaaca ggctttcccg gtaatttctc gaccttttta tcttccacta
    16441 atctcgtcaa ataatggctg gcattctgac gagagatacc tagattcttc gaaattttct
    16501 cggcagtgac agaagacata ttccctgtta gtcgtatcag ctcatcaaaa actcgatctt
    16561 tagcgctctt tttcattttt tcctccagtt taatgtgtct aaaagcataa taaactatcg
    16621 gctgcaatat gtctattcta tatttgtatt ccaattttta ctttgaaaca caaatagtcg
    16681 aatatttgcc tcagatatct gacaaaataa tttatctacc aatttagttt ttatctaata
    16741 tgatttttat gttttttcat agctacaaat attgaactgg attcatatat tagttttatt
    16801 aatagattac agaagggtta agtatatttg catagtttcc aattacttta tccttctgta
    16861 actttttcaa tcttagattt actagcccgt ctcgggccct attttacaat ggttttgtcc
    16921 acttttctct ggtagaaaaa cgtggtagaa aaaataaaat aacacaaaaa aagcggcgtt
    16981 tcatccgctt ttggtagaag attggaaaac tcagatagaa aaaggacgat ttttagctct
    17041 ttagaacgtt gataagtccc cattcttact gagttttgcc attatctcct atacatcttg
    17101 aattctaaga ataggtcatt atagatgcct aggtatttcg atcctagatc tttgtagact
    17161 tcaagatggg agatcgtgtc atcagatgga tagaattgtt cgtccgacga aatgtctttt
    17221 ggcaataacg cttttgcttc ttcatttggt gtagaataac caatatactc tgcattttgt
    17281 gccgcatttt ccggttctag cataaagttg atgaaatcat aggcaccttc tttatttttc
    17341 acggtttttg gcataacgat attatcaaac caaagattgg atccttctgg tgggatcaca
    17401 taatgtaaat gttcattttc ccacatcata tcggcagctt cgccagaaaa tgtcactgcc
    17461 acggaggctt cttcattgat catatacatt ttgatctcat ctgccacgat tgctttgaca
    17521 tttggtgtta aatgattcaa tttatttgct gcttctgtca gctgtgccat attcttacta
    17581 ttcaaagaat agcctaaact attcaaagaa agccccataa cttctcgtgc accgtcaatc
    17641 agcatcaaac tgtcttttaa ttccggcttc cacaaatcat cccaatgctt aatttgattt
    17701 gcatcaaaga atttatcgtt atatacgatc cctaaagttc cccaaaaata tggaatcgaa
    17761 tactgattat ctggatcaaa atccaagtct aaaaatcgtg gatcaatatt atttaatcct
    17821 ttgatcttcg aatgatctaa tggaagtact agtttttctt tgatcatttt ttggatcata
    17881 tattcacttg gtatagcgat gtcgtagttt gtcccacctt gctggatttt tgtgaacatc
    17941 gcttcattag aatcaaatgt ttcataattc actttgtatc catattcttt ttcaaatttc
    18001 gtgatcaaac taggatcgat ataatctccc cagttataga tggtaagtac ctttgctcca
    18061 ctcatgccac ttgttttttc aagttgacga acaccaagca gcagcaccgc aataatgatg
    18121 agaataccta aaaacagaga ttgtaatttt ttcatcgcag ccgtgccacc ccctcgcgta
    18181 ctcttctgtt tttcttcagc cgttttggac ggttttcttt actaataaag tagtatccaa
    18241 tgaccaatat catggagaac aagaaaacta acgcacttag cgcattgatt tctaaactga
    18301 ttccttgtct tgcacgagaa tagatctcta ctgagagcgt cgtaaagcca tttcctgtga
    18361 cgaaaaatgt aaccgcaaaa tcatcaagag aataggtaaa tgccataaaa tatccagcta
    18421 tgattcctgg agaaagataa ggcaaaatga tatttttgac tacttggaaa ttattagcac
    18481 caaggtctct agccgcatcg accattgaat cgttcatttc ctgcaatttt ggcaagacca
    18541 tcaagacgac gattggaatg ctgaaagcaa tatgggaaag caatacactc tgaaaaccta
    18601 atccgaatcc tagtagtgta aagaaaatca agaaactagc accgataata acatcaggag
    18661 agactaataa gatgttattc atgctcaaca acgctgtccg tgcacgtcgt tttttcgtgt
    18721 aatagattcc catagcccca aatgtcccga tgatcgtggc gatcaatgca gataagaaag
    18781 ccagcatgaa cgtattcaat acgataatga tcaaacgtgt atcttcaaaa acagcttcat
    18841 aattttccca agtaaatcct gtaaagctgt tcatcgttcc tccttcattg aaggaataaa
    18901 aaatcagata gaagatcggt gcatataaga ataaaaagac gatccctaaa taaagatacg
    18961 accatttaaa ttttttcatt tatgagcgac ctcctttttt cttctcgcct gtcaggagca
    19021 tcacgatgaa catagcaatg attaaaatca caccgattgt agaacccatc ccccagtttt
    19081 gtgtaaccaa gaaatgctgt tcgatagccg ttcctaaagt gatcacccga tttccgccaa
    19141 tcagacgagt cagcatgaat aaagaaagag aaggaataaa tactgcttgg acaccacttt
    19201 tcacaccatt taatgataac ggaaaaatca ctcgtcggaa ggtctcaaag ctattcgcgc
    19261 ctaaatctct actagcactg atcaaagaag gattcatctc ttccaatgca ttgaaaattg
    19321 gcataatcat aaatggaatc tcgatatacg ttgccacgaa cagaaaactg aagtctgtga
    19381 ataatatctg tttcggcccg atcccgataa aatcaaagaa atgattcacg ctgccgtgca
    19441 cactgaaaat cccaataaat gcgtaagctt taagcagcag attcacccat gtaggcaaaa
    19501 taatcagcat cagccataat tgcttgtgct tcagttttgt taaaaaataa gcagtcgggt
    19561 aactgatgat cagcgtgaaa aacgtgatca aaaatgcata ccatacagaa ttgatcgtca
    19621 tagaaagcga tgtccccgac gtaaaataag atagataatt attcagcgtg aattgcccat
    19681 tcatatcaaa aaaagattga taaatgatca ttaatacagg cgcgatcacg aataaaccga
    19741 gccacaaaat ataagggacg gaatagatcc tgcgcattgt tttcattctt gtccccccta
    19801 ttcttcgtag ctgtcaagac gtgcatcgaa ttcttcttca gactcgttga aacgcatgac
    19861 atggatatct tctggctcaa aggccaatcc tactttgctt ccttctttag cttttttcgt
    19921 tgaatggacc atccattcat tttgctgttc atcataacag atgatttcat aatggacacc
    19981 acgaaacagc tgagtatcga ccgttacgac tagttttcct ttatctggtg tcgtgatcgt
    20041 caaatcctct gggcgtaaaa caacttcaac tggttcattt agacgcatcc cgccatcgac
    20101 acattcaaat tgcttaccaa caaactcaac tagattatct tcgatcatta caccgtccac
    20161 gatattgctc tccccaacaa aatcagcaac gaaatgattg attggctcat cataaatatc
    20221 tacgggtgta ccactttgga cgattttccc tttattcatg acaaaaatct catcactcat
    20281 tgccaacgct tcttcttggt catgagtaac aaaaataaac gtgattccta atcgttgttg
    20341 cagatcccgc agttcatact gcatatctgt acgtagtttc aaatcaagcg ccgataacgg
    20401 ttcatctaat agcaatactt tcggttcatt tacaattgct cgtgcaatcg ctacacgctg
    20461 tctttgacca ccagacattt cactaatctc ccgtttttcg tatccaggaa gctggaccat
    20521 acgcaaagca tcttttacct tcttttcgat atctgatttg gacattttct tgatcttcaa
    20581 tccaaaagca acgttatcaa aaacattcat atgaggaaaa agtgcataat cttggaagac
    20641 tgtatttact tgtcgcttat tagctggcaa atcattcaac cgtttccctt caaaatagat
    20701 gtccccttct gtagcttcgg tgaatccagc tataatacgt aggatcgttg ttttaccaca
    20761 gccggaagga ccaagcagtg tataaaattt tccttgttcg atctcaaaac ttactttttt
    20821 taatactgtt tcatcatcat agcgtttcac tacttgatca aatgaaatga ttggtttccc
    20881 cacgttttta ccccctatat tacaaatatg attgcgttgc aacaatcaat acttttgttc
    20941 gttttttgag atgattcctt aattgatgtt gttttgtcgc ttgataatag atcgactgtc
    21001 ctgcttttgc cacatagacc gtctctccta agcttaactc taattcccct tctaatacaa
    21061 aaataaacgt ttcagataat gaaggttcaa acgtcttata ttctcctgca ccttcaaaag
    21121 tcagcaagac aggctccatt tctttttcat tcgaatcagc cacgagccat tccaattcat
    21181 acccgttttc ttcatcgaaa taaaccgtat gatcttcttt tgaataaacc acttgcagat
    21241 tcgctgcttc ttgatgaaaa aattcttctg gagtcacacc taagacttca agaatagaaa
    21301 agaatgtttc cattgatggt gaacttaaat ctcgttccaa ctgggaaata tacccttttg
    21361 acaaatctgt gcgttcgcct agttcttctt gtgtcaaatt cttttggatg cgcaaatttc
    21421 ttaacttctc accaatttcc ataatttcct ccgaatcgaa gttttctaat agtaaacttc
    21481 ctgtttacta ccactcgtat tagtatacga aatttattga aaaaggcaac cctttgtaaa
    21541 attttttttg ctgaatattt ctttatcttt tggaataaat tctagttgtt tgattcttta
    21601 tttttcacct aatttttgtc taaatttacg gaagaacaag aataaaagag taggctttat
    21661 ggtatgataa aagaaaattt taaaaggagt gtctcacgaa tgaacgcttt tcgatctttt
    21721 caggtaataa aggaacctgt tttccataca gcaatcagtg atctgccatt tactgagttg
    21781 gctgaaaatg aagtcttggt aaaaattgcc tattctgatg tcaattataa agatgcattg
    21841 gcaatgagcg agtctggtca ggtgatacga acatatccga tgactccagg gattgattta
    21901 tccggaactg tcgtccaaag taacaatcca cgtttttcaa aagaagatct tgtacttgct
    21961 actggttttg ggcttggcgt cacccatcca ggcggttata gccaatatca aaaagtacca
    22021 ggagactggt tagttcctct accaaaaaat atgacgctgc gtcaagcgat gatccttggt
    22081 acagcagggt ttactgctat gttgtgtgtg aacgctttaa tccgtcaagg tatgacctcc
    22141 gataaaaaag ttgtcgttac tggtgcttct ggcggtgtag gcagtacagc gatagccctg
    22201 ttacataaac taggattcca gtcaatcatt gccttttcca gaaaagaaga gtctgtcaca
    22261 tggctaaaaa gtctaggtgc tttgcaagtc gtacgacctg atgaatttct gccagagaca
    22321 acaaaagctt taggaaaaca gcaaatcgac tatgtcatcg acacagtagg tggcgagcag
    22381 ctaagcagtc ttcttccatt gatctcttat aacggtgcag tagctttatg tggcaatgct
    22441 gggggaatca aactaaacgc gactgtcttg ccttttattc tgcgaaacat ccagttgatt
    22501 ggaattgatt ctgtcaatgt accaatagat caacgtcttt ctttatggca acagatggcg
    22561 gatttgcaga ttgcagatga actggtcgtc caagagatta ctctggatca attgcctgaa
    22621 acagcatcta aactacttgc tggaacacac caaggaagaa cactagtaaa tgtaggtgac
    22681 cataaatgaa gattctaatc acagctggag gaacgactga aaaaatcgat caagtacgtg
    22741 ctattacaaa tcattcgaca ggacgattag ggcaagcatt agctgattat ttagctgcca
    22801 atccggatac cacaatcgat tacgtgacta cgaaacaagc actgaaaccg aaaagacact
    22861 ccaatataac gatttatacg atcgaatctg cactggactt atttttacaa ttagaagaac
    22921 taacgaaaaa agaacactac gatgcgatta tccacagtat ggcagtcagt gattttactc
    22981 ctgcttttag tttttcagaa gaacagctgg caaaaaacct acccacttct tcaacgcaag
    23041 aagaactgga caattggttt gcggaaaatg aacagacaga taccactgtt tcaaagatct
    23101 cttctaacac cgagcatctt gtgctcgttt tgaagaaaac accaaaaatc atttcttatc
    23161 tacgtgaatg gcagccaaaa gcaaaaatca ttggtttcaa actattagta gatgtaccaa
    23221 aggaatctct tctggcagtc gcaaaaaata gtctgataaa taacaaaact gattttattt
    23281 tcgcaaatga tttgacggaa atccatggcg agacacacca cggctatctc ttatctaaag
    23341 atggaacagt ggaagaagct caaagtaaat ctgagattgc tgcattgatt actgagaaaa
    23401 ttagactgga ggaatcaaaa tgaaaaagat cttactaggc gttactggaa gtatttctgc
    23461 ttacaaaagc gctgatctta caagtcaatt cgtcaaatta ggtgcccagg tagatgtgat
    23521 tatgtctaaa agcagcacac attttatcac gccattaacc ttgcaatctc tgtcgaagcg
    23581 cgcagtccat acagacgtca tgcaagaatc aaatccgcag gtaatcaatc atatcgaact
    23641 agcaaaacaa gctgacttgt ttctcatcgc gcctgctaca gcaaacatta tcggcaagct
    23701 agcaaacggc attgcagacg atcttctttc aacagtcgca atggcgcttc cagtgggaac
    23761 accaaaattg attgcccctg ccatgaacac gaatatgtat caaaacccga tcactcagcg
    23821 taatctggaa acattgagat ctgtcggcta tcaggaaatt gaaccaagag aatctctttt
    23881 agcttgtgga gattttggaa aaggtgcctt agctgatatt cagacgatca tcgaacaggt
    23941 catgctgact cttaatgaaa gaaatgctta gaaaaaagga gtacttatga aaaatacaaa
    24001 aaactttaca ttaactgcga tgtttttagc tattttgatt ttattagctg ttacgccttt
    24061 aggttttatc cctatcggtc caatcaatgc aacaaccatg catattcctg taatcattgc
    24121 ttcgatcgtt ttaggaccaa gactaggtgc ttttcttggc ggtacatttg gactgatcag
    24181 catgatccgt agcacagtta tccaaacacc tttgtctttt gtcttttctc cgtttatccc
    24241 agtcatcgga acagaacatg gaagctggaa agccttgctg atcgcgttta ttcctcgcat
    24301 cttgattggg gtggttcctt atttcgttta taaaggtctg cgcaaactaa tgaaacaaaa
    24361 agcagattcc gtttctttat tcttagctgg actttccggt tcattgacca atacgatttt
    24421 agtgatgaat atgatctact ttttattcca gcaagattat gccaaagtca tcggaacaaa
    24481 tttaaatgct gtttattcag ctattctagc ggttattttt acaagtggta tacctgaagg
    24541 aatcgtcgca ggtcttgcga ctgcagcagt atgcaatgtc ttgttaagat acttcaagca
    24601 cccttctctt actaactgat agtcatcgta gccgatctct gatttttttc tcttttggtg
    24661 ttactattga atggaaagat ttgacgtcga aaggatgatc caaatgaaaa tttttgttgt
    24721 aggtgcaaac ggacaaattg gccgacacct gataaaagat ttagctccaa gttcccatga
    24781 aatttttgca ggggtgagag atgttgcttc ccaaacatta gtaaaaaaag agaatgtttc
    24841 gtatgtctct tttgatttaa cttggtccgt ggaaaaaatg accgaagcat tcaaaggcat
    24901 cgatgtattg atttttgctg ccggctctca aggaaagaat cttctgcaag tagacttgga
    24961 tggcgcaatc aaaacagtga tcgcagctga aaatgcgcat gtctctcgct atctgatggt
    25021 cagtgctgtt tatgcagatg aaccagcaaa atggcctgag tccatgacag attattatat
    25081 tactaaacat tatgcagatg aatggctgaa acgaactaac ttagattttg tcattcttca
    25141 accagtcaca ttgactaacg atgaagaagt tacttccatt caattaacta agcctaatga
    25201 gaaggcatca aaaacgatca ctcgctctac agttgctgct gtacttgccg ctctagtgga
    25261 agaaacggat atttctcgta cgactctagt actttctgaa ggtagcaaag agttgaatac
    25321 agcattccaa gaatgggcaa aggaggaata gatccatgcg tgccatttca atcaagcatc
    25381 ctggttctgt agatgtatta gaacaaattg aagtgcctgt tcctaaacca aaacaaggcg
    25441 aattactgat caaagtccat acagcagctg tcaatcgaac agatattatg aaaagagaat
    25501 cagtctcttt gaaacctccc taccctattt tgggagtcga agtagctgga gaggtagttg
    25561 agaataaaag tgatatctcc acttttactc ctgggacaaa agtatacgga ttagtcaatc
    25621 taggtggtta tgccgaatat gctgtgatgc ctgccgatcg tgcaatcctt ctccctgata
    25681 cgctagacta tgtttctgct gctggaatct cagaagtttt tttgactgct tatcaaacac
    25741 tttactggct tggaaaatta caagaaggcg aaactgtctt gatccatgca ggagcaagtg
    25801 gtgtgggaac agcagcgatc caattagcta gacagctttc tcacgcgaaa atcatcgtta
    25861 ctgcaggatc agaagaaaaa ctcgcctttt gtaaagaact tggtgcagat gaattgatca
    25921 actataaaaa acaagacttt caagaagagg tacgaaaaat cactgaaggt cggggagtcg
    25981 atgttatttt ggatttcatc ggcgcttctt actgggaaaa gaatttagct tctatagccg
    26041 ttgatggacg ttgggtcttg attggtatgc taggtgggac catcgtgcct gagatagacc
    26101 ttggtgtatt gattgggaaa cgtgtccaac tgcttggtac attattgaca cctagaagtg
    26161 atcagtataa agcagagctc gcacaaactt ttatgcaaaa agctgggcct tttctagaaa
    26221 atggcaagat tcggccaatc attgaccgta cattccaatt cagcgcagtc aaacaagcgc
    26281 atgaatatat ggaagcaaac aaaaacatcg gaaaaatcat tttgaagata actgaataaa
    26341 atcatgctga t
//
LOCUS       DACEKH010000031        26162 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEKH010000031 DACEKH010000000
VERSION     DACEKH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.031"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(64..1095)
                     /gene="queA"
                     /locus_tag="IW662_001209"
     CDS             complement(64..1095)
                     /gene="queA"
                     /locus_tag="IW662_001209"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ0866044.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1288..2463
                     /locus_tag="IW662_001210"
     CDS             1288..2463
                     /locus_tag="IW662_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0866045.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            2467..3108
                     /locus_tag="IW662_001211"
     CDS             2467..3108
                     /locus_tag="IW662_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0866046.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3105..4022
                     /locus_tag="IW662_001212"
     CDS             3105..4022
                     /locus_tag="IW662_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0866047.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4019..4690
                     /locus_tag="IW662_001213"
     CDS             4019..4690
                     /locus_tag="IW662_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0866048.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5232..5720)
                     /locus_tag="IW662_001214"
     CDS             complement(5232..5720)
                     /locus_tag="IW662_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ0866049.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVSLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRTAKSSCDGC
                     SP"
     gene            complement(5921..6187)
                     /locus_tag="IW662_001215"
     CDS             complement(5921..6187)
                     /locus_tag="IW662_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0866050.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6187..6450)
                     /locus_tag="IW662_001216"
     CDS             complement(6187..6450)
                     /locus_tag="IW662_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0866051.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDNIDWEAL"
     gene            6925..7110
                     /locus_tag="IW662_001217"
     CDS             6925..7110
                     /locus_tag="IW662_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0866052.1"
                     /translation="MALSYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7252..9075)
                     /locus_tag="IW662_001218"
     CDS             complement(7252..9075)
                     /locus_tag="IW662_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ0866053.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="IW662_001219"
     CDS             complement(9671..12376)
                     /locus_tag="IW662_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0866054.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSENLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIEAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGVLSKADQIDFESLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPTLKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12682..13629)
                     /locus_tag="IW662_001220"
     CDS             complement(12682..13629)
                     /locus_tag="IW662_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ0866055.1"
                     /translation="MRRFAIKGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13819..14088
                     /gene="rpsN"
                     /locus_tag="IW662_001221"
     CDS             13819..14088
                     /gene="rpsN"
                     /locus_tag="IW662_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0866056.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14264..14626)
                     /locus_tag="IW662_001222"
     CDS             complement(14264..14626)
                     /locus_tag="IW662_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0866057.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14637..15758)
                     /locus_tag="IW662_001223"
     CDS             complement(14637..15758)
                     /locus_tag="IW662_001223"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ0866058.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQIKKLPAGEGIGYGETYITSE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15774..16124)
                     /locus_tag="IW662_001224"
     CDS             complement(15774..16124)
                     /locus_tag="IW662_001224"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ0866059.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16254..17765)
                     /locus_tag="IW662_001225"
     CDS             complement(16254..17765)
                     /locus_tag="IW662_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0866060.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18087..19439)
                     /locus_tag="IW662_001226"
     CDS             complement(18087..19439)
                     /locus_tag="IW662_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ0866061.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19592..20668)
                     /locus_tag="IW662_001227"
     CDS             complement(19592..20668)
                     /locus_tag="IW662_001227"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ0866062.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLIFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20864..22609
                     /locus_tag="IW662_001228"
     CDS             20864..22609
                     /locus_tag="IW662_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ0866063.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRIPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAKNGTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTRASQKTETVHEKEFGSFTFRASTSTEDPVAPYTVNYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGVFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWSSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22858..23325)
                     /locus_tag="IW662_001229"
     CDS             complement(22858..23325)
                     /locus_tag="IW662_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ0866064.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVIIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGLRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAIDQGLPSTEDSR"
     gene            complement(23309..24682)
                     /locus_tag="IW662_001230"
     CDS             complement(23309..24682)
                     /locus_tag="IW662_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866065.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLSWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFILAKEMGEPIHLLLITLNRESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25006..25494)
                     /locus_tag="IW662_001231"
     CDS             complement(25006..25494)
                     /locus_tag="IW662_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0866066.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25516..26118)
                     /locus_tag="IW662_001232"
     CDS             complement(25516..26118)
                     /locus_tag="IW662_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0866067.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLLFFFVLILYAYHHGIFRSSTSLQV
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7499 a         5559 c         4834 g         8270 t
ORIGIN      
        1 ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg gcttttcata
       61 accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct cttcaatcgc
      121 atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta acatgactaa
      181 agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga attcatagcc
      241 aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat taaatttcgt
      301 tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt tcccgccatt
      361 tttacgtact tggttcaatc gctcagctgc ttcttccgtc aaacggtaga attcactatg
      421 catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta accctacatg
      481 tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta attctttcgt
      541 aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat agacagtttg
      601 atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca tttctcccaa
      661 tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc gtccgccgtg
      721 ttccaattct tctttcacta ccgcttgcaa tctgccatca ccaaaagaga ttcttgtccc
      781 aactttggca cgtttcgcag gttttatcaa tgtctcccac gtatctcctt gtgtattcgt
      841 tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata aacgagcagg
      901 cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt caatgatttc
      961 atggaaatgt ttatcttcca tctctccaat tttccgatcc aaaacaagta aacgagaatg
     1021 atcgcggtct tttaatggtg tttgtgcgat caactcttcc ggcaagtcaa aatcaaaatc
     1081 ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt ctatcatgtt
     1141 tttcattctg ttggcaaaat cagcactttt tcacttctat tttttgtttt cctaaataaa
     1201 aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag atagttcttc
     1261 attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc aaaaatttat
     1321 aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg tgagttcatt
     1381 tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat caaccgaatg
     1441 actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga aattgatcca
     1501 gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat gcctcacatg
     1561 acgatccgtg aaaatatcac ccttgtccaa aaattgttga aagtcagtca agaagaacgg
     1621 aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat gttagatcgc
     1681 tacccacatg aattatctgg tgggcagcag cagcgtattg gagtcgttcg tgcccttgca
     1741 gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc catcactcgt
     1801 gattctttgc aagaccttgt caaagattta caagagcgct tgggaaaaac gatcgtattt
     1861 gtaacccatg acatggatga agcgattaag ctagcaagcc gtatcgccat catgagtgaa
     1921 ggccgtgtga tccaatttga tacaccagaa aatatcttac gtcatcctgc aaacgatttc
     1981 gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc gacaagagtc
     2041 ggagaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt acaagaagca
     2101 atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa ttcgaatgta
     2161 ttaaaaggat ttatcgatgt agaaacgttg aataaaaagc gaggaaaagt atccagtgtc
     2221 ggcgacatat taaataaaca tgttttttac gtaaaagaat ccgcctatct tcgtgatacc
     2281 ttgcaacgca ttttaaaacg cggactgaaa tacgtgccgg tcgttgatga acagaacaaa
     2341 gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt gatttgggga
     2401 gaagatcccg caactactga tgttgcagag tcagatactc ctgaagaatc taaggaggtc
     2461 tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa aggttttgaa
     2521 catctttata tttcgggact tgctttgatc attggtatca tcattgctgt accgcttggt
     2581 gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac cagtgccttg
     2641 cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg cgtaggtaaa
     2701 ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg taatacgtac
     2761 attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat ggggatgact
     2821 gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat catggctggt
     2881 attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta tatcggcgct
     2941 ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga tctcattttg
     3001 gcaggtacga tccctgtaac gattttagca ctattggctg attggcttct aggtctcttg
     3061 gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa cgtatactaa
     3121 aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttctttc cctggtcttg
     3181 ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca gaagcacaga
     3241 tcttagcaag cctagttgcc ggaatggttg aacattatac agataaaaat accgcaatca
     3301 tcaacaatct ggctaccaca actatcaacc atcaagcgat gatgaacgga gatgcttcaa
     3361 tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt cctcctgaaa
     3421 aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga tacgatcaaa
     3481 cgtggttccc gtcctatggt tttgaaaata cgtatgtctt tcttgttcga aaagacaccg
     3541 cacaaaaata ccatttatct aaagtcagcg atctgaaaaa tgtagcggat gaattagtag
     3601 ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc ttccaagaaa
     3661 cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt tatgacgcag
     3721 tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg atcgccagtt
     3781 atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat gctgctccag
     3841 ttgtaaacaa caaaatatta gctgatacgc ctcatttaaa agaagcctta aataagttag
     3901 gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat aataatctag
     3961 tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt gaagggaagt
     4021 gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc aggaaaatgg
     4081 ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg gtgtcctctt
     4141 tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta aattagcaaa
     4201 ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca tgatctccat
     4261 tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat ttttgtattc
     4321 cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca aaaacatcct
     4381 tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg tagagttacc
     4441 actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg ccatcgggat
     4501 aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta tccgaggaac
     4561 caatgcaaca gatggcacat cgattatttt agcaggtgca ttgccaactg cattaatggc
     4621 gattatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag ccagccgtac
     4681 atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg gtcgcaaagg
     4741 cttttttagt tctgtcattt tttaaggcaa taatccactc tgattcttcc ctccttatgg
     4801 gcaaagagtt gcttttctta gctatttatc cattccgtat actccaactt catactgcca
     4861 acgggttcat ctctgcatcc accgactaag tttttttctt atccgctaag gcattttgcc
     4921 aatccatttt cccatcactc gtcatggaat tcataccaca tgatctctaa atactcgtct
     4981 gcttgttctt tgaattgggc atcaattctt acatcctttg gcgtggcttt catttaagcc
     5041 ttttccatca gtcctcgatt gttcgatgcc ttcagctaca tcatcaaaac tggtgactgc
     5101 cgtatcggta acttcgatca tcttttatac aatcgggaac ttgccatcct catccttggc
     5161 taaaaacata tctagccatt ccagcttctc gccatgggag gtttgcggtt cctaaaaccg
     5221 gtacacgatt cttatggact gcatccgtcg caactagact tggcggtacg atgatccttc
     5281 accagaagag cacccccgta aaccaattga tcgatatatt gccaataaga aaagacattg
     5341 gcgtaaaaga tatcgatccc tcgtgatgca ttgccgttga tactgctatt catggccgaa
     5401 agcgccacga ctttgacttc ttccgtttga gtgacattca tgttcggcaa tgtcgctttg
     5461 gatacccgct ctgctaaaga aacgacactg gtattgtatt tcgtatctgt atcttttcca
     5521 aaagaccatg ccaacgaatc ctctgggaac caataagacg actccggttg attgtccatt
     5581 gcttttttca acatactttt ggctgtttct cgataaacga catttccaat tgtcttcgcc
     5641 tcactttggc gataatcgat ggtcgttgcc actgccaaca ctaacaacga tgctccaatt
     5701 ttactgattt tccttgtcac acttcttcct ccttgtttta cataaacccg tcaacattcc
     5761 attcctaaac tcaaacaatg acccttcaaa ggtggagata taggaacaaa ttaaagaaat
     5821 catgcaataa atccaagaag gttggtttta gaacaaaaaa agagtctcct ttttggggag
     5881 actctttgtt aactatctaa aaaggttggc tcttctcact tcactcataa tgtgtccagg
     5941 cagaataaat ttccacaact ttattttcct catcaacttt gtaaaccact cggtgctgtc
     6001 tattcagcct gcgtgagtat ctgccttcat gtgtaggtct cagcttttca aaagactgcg
     6061 tgggcatata tggatcttcc ttcaatgtct gaatcacttc ttcaaattgt ttctttaaat
     6121 tcgattgctt gatttttctt aaatccactt tcgcagagtt tttgattgcg actgtatagt
     6181 tactcatcaa agagcttccc aatcaatatt atcgatattc gtaaagccac tgtcgtcttt
     6241 ttcgcggtct cgtactttat ctaaagtacc tgtttgctca agaaaaaggg tttcttgaat
     6301 cgctctccag tcttctagcc caatcaatac agcattgttt ccactacgat cactaataat
     6361 ctcgatttca gtatgatttt catttacctc tttcaataac tgataaaaat ttttacgagc
     6421 tgtgctagga ttcatcgttg tattagccat tgtctctcct cctttttctg atataattat
     6481 aacgtacttg attaagtacg tcaaaaaata caataaagca cttaaattca gcaaactttt
     6541 cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc cacgctcact
     6601 tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca acgtatcatc
     6661 tttagcaaag gtcaaatcca aagcctgtga gtctatgcaa caacaccaat acacgaaggt
     6721 tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg cacgaaagat
     6781 ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct caaaaatgat
     6841 tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc ttagaccgtt
     6901 gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa atggtcactt
     6961 gacgaggctg agatttctac ttatgaagac acatggcact taagagtcga ggtaaaggaa
     7021 gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa ttatgatcaa
     7081 gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt tcatacgctg
     7141 ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca aaacagaccc
     7201 tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg tttatttctt
     7261 caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc gcaatcttaa
     7321 ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta caaattgagg
     7381 aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga tatctacata
     7441 acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa aacggacatc
     7501 tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat cttcgtctag
     7561 cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca acgcaccgat
     7621 atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat aatcatgtag
     7681 ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat catggatctt
     7741 ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt cccctaaccg
     7801 atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacctgtaa tattggcaat
     7861 cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc ctgtttgaga
     7921 taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt ctgttgagcc
     7981 atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc cattggaata
     8041 gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt ttgccagatt
     8101 gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta aaatcagcgc
     8161 tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt taccaatttg
     8221 agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga taaatgtgat
     8281 ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag cggcattttt
     8341 tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc cagttaaaga
     8401 ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg gcacagccgc
     8461 tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat ataaaccaac
     8521 aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata ggaacgatgc
     8581 agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg tcacagaaat
     8641 agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag cacctgctga
     8701 gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg aaatcaagcc
     8761 agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat gaggataagc
     8821 atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa caaaagcggc
     8881 aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga caacgatttg
     8941 ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca aagcagcaaa
     9001 tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta ctaacaacct
     9061 ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag ttttatagtc
     9121 gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag tcaataaact
     9181 ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa cgctttaata
     9241 cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat aaaaggatag
     9301 aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg aaagagagat
     9361 gtaaggttgc tttctaccca tcattcttac tactcgaagc atagcgcagt tgtcacaacc
     9421 tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc aaattaaaaa
     9481 cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag attttttaca
     9541 acctcttggt acacggtgtt caatcagctg accctcggca ttagctcaaa aaacggcaaa
     9601 atatgaggaa ctatttttcc attttttctc taatactcag gtcaaaacgc tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccagtaaat gcctcctgca gattcccgca
     9841 acaagtttac cgcatgcgta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcatctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggatgccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ctctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgttgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaagga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagcttagca
    10801 gagattcaaa atcgatctgg tccgctttgc ttagcacccc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg cctcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgcaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatt ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta
    12601 tacgcaaaaa agctaaagca ttttttgtct ttgcccctac aaaaatgttt cagctttttt
    12661 cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg
    12721 atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat
    12781 tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc
    12841 tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg
    12901 caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg
    12961 gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc
    13021 agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt
    13081 ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg
    13141 gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc
    13201 tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac
    13261 cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg
    13321 caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac
    13381 atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg
    13441 tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc
    13501 ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct
    13561 gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaacctt taattgcaaa
    13621 tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac
    13681 aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaataattgt cttttttcgc
    13741 gaaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat
    13801 aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa
    13861 gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa
    13921 ggcttgcgca aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc
    13981 gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa
    14041 ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc
    14101 tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc
    14161 gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg
    14221 gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag
    14281 tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc
    14341 gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca
    14401 acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg
    14461 gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat
    14521 ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca
    14581 cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat
    14641 tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta
    14701 cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc
    14761 gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat
    14821 acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt
    14881 cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc ttcagaagtg
    14941 atatatgttt ctccatagcc gattccttca cctgctggga gttttttgat ttgaatcaat
    15001 tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag
    15061 ggattcagtc cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc
    15121 cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttt
    15181 agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct
    15241 gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca
    15301 gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct
    15361 tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgcc
    15421 tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt
    15481 gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac
    15541 tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc
    15601 tcagctgttt caatagctcc atgaccgtat ccgtttgctt ttactactgc aaacaattct
    15661 tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt
    15721 atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct
    15781 tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg
    15841 ccttcatgag gtgaacgcgt cacaacaggc tgaccgtttt cattttttaa aatctcgaca
    15901 tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa
    15961 gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc
    16021 atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg
    16081 atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc
    16141 aataaattta gaacactctc attctaccaa attataacca ttttgccaca aataagtcca
    16201 caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg
    16261 ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt
    16321 tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg
    16381 tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc
    16441 tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt
    16501 gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta
    16561 tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca
    16621 actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc
    16681 atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg
    16741 aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag
    16801 ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat
    16861 ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc
    16921 aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg
    16981 cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt
    17041 gttagttcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct
    17101 tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc
    17161 acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt
    17221 gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct
    17281 tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt
    17341 ccaagtttca atgtgtggcg gttgatgtgg tcaagcatac gtcctggtgt acccacaacg
    17401 atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg
    17461 gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata
    17521 gctaactcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt
    17581 ttttctaaca tcggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca
    17641 ataacgtctt ttccagctaa tgctagtgga atcgtttctg attggatagg tgttgcttct
    17701 tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat
    17761 ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc
    17821 gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca
    17881 ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc
    17941 caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct
    18001 atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt
    18061 ttcgttttct gcaagtttac agatttttat ttcatttgct gaagctgttc taccacttca
    18121 aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt
    18181 tttacagcat caatcaattt attcttttct ttttcaaaat agaacacagg tagttggggg
    18241 tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg
    18301 gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc
    18361 cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg tagattttta
    18421 aaagtatcta ataccagtcc catggctgtt ggatttgcat tatagacatc gctcaaaata
    18481 gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg
    18541 agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc
    18601 aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact
    18661 acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca
    18721 ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca
    18781 tctgcgggca caacgagtaa tccgtctggt acaagtccgt cagtgatttc cattttggct
    18841 ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt
    18901 gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg
    18961 cccatttcca agacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga
    19021 ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca
    19081 gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct
    19141 gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct
    19201 acaggaataa ctggaaaatc ttttggagct tgttcaacag gcaagctcca aaatgaagca
    19261 gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga
    19321 ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt
    19381 gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt
    19441 gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg
    19501 actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac
    19561 gcaatcactc cagcttcttc tttatttctc tttattcatt tttttcaaaa aaagattgac
    19621 gctgatggta tcgattcatt cctaattgaa tcagttcttc aatcaaatca ccgtatttca
    19681 agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagaat
    19741 ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcacac cggctcaatc
    19801 cgcttccgcc taacatcgtg taagctaact tcgcgtactc ttgtgctttt tgataaactt
    19861 cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat
    19921 aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat
    19981 ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag
    20041 aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg
    20101 taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac
    20161 cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta agtactggta
    20221 cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca
    20281 tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga
    20341 agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc
    20401 cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc tccgtctgtc
    20461 cacttgggtc ccatgaaaga tgcaagacat ctttgcttgc aggtttttct gttagtaatg
    20521 gacctttgac ccattgtcct tcttttgtaa taaatatgag ttgaacttgg taataattat
    20581 aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc
    20641 gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag
    20701 tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa
    20761 acgagttggt ttctaaaaga acaaacgtca tttatttttt atcagctaga ttatttgtcc
    20821 cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc
    20881 acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag taccaaactt
    20941 tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt
    21001 catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca
    21061 aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac
    21121 gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt ccaaatcgcc
    21181 ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgactcggt
    21241 cgtcgttaag atccaacacc ctaacatcgc cgaagagatt cgacttgatc ttcaactatt
    21301 tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg
    21361 ggtactccaa gaagtgaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca
    21421 aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca tccccaaaat
    21481 ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt
    21541 gaatcatttg atgaatgcca aaaacggaac agaaactttt atcaccggta ttcaaaacaa
    21601 gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac aggtatttga
    21661 tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa
    21721 agaagaacag acacgagcta gccaaaaaac tgaaacagtc catgaaaaag agttcggttc
    21781 ctttactttt cgagcctcta cttctacaga agaccctgtt gcgccatata ccgtcaacta
    21841 catcgatttt ggtatgatgg ggcatctttc tgcagggctt aggcaaaaac tcacacaagc
    21901 cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtct tgcgactttg
    21961 tcagcaagaa ggcgtgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga
    22021 acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca cacacgtcgt
    22081 gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc
    22141 attcgggaca ttagaagggg tgatccgtgt ccttgaccct gaagtttccc tcatggaagt
    22201 cgcttcgcct tttgcccaac attattttct cactcatctg gatgtagaag atacattgaa
    22261 acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct
    22321 ccaccatcta ttagaaatgt ggtcttcggg acaaggaaaa gtcaatttgg aattgaaaaa
    22381 gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat
    22441 tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc
    22501 agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt
    22561 tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt
    22621 gacagaaaga atcagcttca agaaataaga aggggtttta cataaattat ttctcaaatt
    22681 tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa
    22741 tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaaggaaca aggaatccat
    22801 ttatcctgtt tccctgttcc ttttttggtg tattcgagca taaataaaat accttcgtta
    22861 cctactatct tctgtacttg gtaaaccttg gtcaattgcc ttttttaacg cttgacgaaa
    22921 agacatatga tctttctccg taaagggttt tggacccttt gtccttatgc cagcttttct
    22981 taactttcca ctatgataac gttgttctag aagcgtgccg atattcgctt cgtcaaattc
    23041 ttttccagag tgatggaaca ctcggaggcc cttcggcaat acaagtgaag ctaaaccata
    23101 atcttgcgtg atcaaaaagt cattcttctt aaccataccg acgattttat aatccgcact
    23161 atcagctccg cggtctacgt agacgatttg gacatgatta gcgtattctt tggtcgtata
    23221 atggtccaca ctggttacga taatcacgtc gagaccatag gattctccta attttatgac
    23281 atcttctttt actggagaac catctccatc aatgaatagc ctcattcttt ttcgccttct
    23341 tccataaatg ataaacagtt actaatccgc tcagtaaaag accaacattg atccattttg
    23401 ccaagataag cagttgtaca ctttctatcg gcactgattc acggttcagc gtgatcaata
    23461 atagatggat tggctctccc atttcttttg ctaaaataaa caaaagcgtc atcaataaaa
    23521 actggtggat cacactccct ttattttgag tagtatactt gacaacgatc cggcgatcca
    23581 ttggaatagc tgcatcagtt tgataatatt ccttatttag ccagctgcct gttactacgt
    23641 gccagaaact aagcagaaag acacctatcg caaaaccatt tggaatcaag aggaccaata
    23701 aagaagaact cagtccaagc aattgcatag ccaatggaga ccatgataaa cgtttcaaaa
    23761 tccatggtcc gactattttt gctgcaatca agcctgctgc ataaggcaaa tacataaaaa
    23821 tcgaaagttt ttctaatccg tacagtatgc caacataaaa actgctaaac aaaaaaagga
    23881 aattccctag cattccattt agaaaagtaa acaaatttag ccagcttggc aacttccatt
    23941 ccttcttctg gtgtcctaaa acaaacacga acaagatgaa cagcagcaag aaaccataaa
    24001 tagcaagatc aaaaagcctt tcatcgaata acaatcgggc atttctcaaa agaaacaata
    24061 agccaaagaa tatcaaaaat aaaacaagct ctcgcgtagc taccagattt cttttgatgt
    24121 ctttgaaatc cagttcgtaa cgcggataat gactaaccgt atggtaggcc ataacgtaaa
    24181 aaagtgtata aatagtaaag cttgctgcaa tctgagtcgg tgcaggcaac tcaatcgctt
    24241 gatacaggag aaatagccag atagctgcaa ataagtactt tttcccatcc atattgataa
    24301 aaccttgcgt cttttcgtga aaattgaccg tggtattagc aggtacaagc catgctgcac
    24361 tcagccctag caaaacactt gaaaaataaa ataaaggaaa ataaaatacg cctaatattc
    24421 caagcaatga acccgcacca ccaatcagca gagcgatcat cagcaacgtg taactatcta
    24481 gaccaaatcg tagactgttg actaaaaata gtccagtcat ccgaaaggca taaaacagcg
    24541 taaatggcaa aatcgtcagc caattatgcg agtctgttag tcccaaaaat aaaagataag
    24601 gaataaaaat aaccgtgtta cttaagataa agggcgttgt ttgtgtaaaa ttacgcccaa
    24661 atcgtttgag tttttcgaac atgacaatgg ctcccttaaa cagtcttata actaatctgc
    24721 tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa
    24781 aaaaaataca gaaagaagca taagcttctt tctgtatttt tacggtactt gattactcaa
    24841 gcagacaagc ccttctccac aaaagtcttg tccgacaccc ctgcatccgg caggtgataa
    24901 agatagaaat caattatcct gtgcctccaa aatcaattag gaactctgta aaccagagtc
    24961 cttctcgacc cgtcgcataa atttacttta acacagttcc tatgtttatg cgagagcttt
    25021 tttcatttta taatcaacca tctggaatcc ttgaccgata tctgaaataa gttcttctgt
    25081 aatctcaaac ccttcatgct tataaacctc gattgcttga tgatttccct ggttcactcg
    25141 caaaaagatc tctctttcct ttgtttgcgc agcaagttct tcataccagc gaaaaaccga
    25201 agaagtcaat ccttggccac gtactttatt tagcaaatat aatttactta aataaatttt
    25261 tccatttatc tcttcataag cagtgtaacc gaccgtcttt tcttcgcttt ttaataaaaa
    25321 gtaggacaca ccttctgtca gttctttttg aatctgtttt tttgactgat aagttttcag
    25381 catgtaatcc acttgttttt gaccaatgat cggcacaaaa gcttccggcc atacttcttg
    25441 tatgactgcc atcagctcat ccagttcatt ttctgttact tttatttttt ccatacaccc
    25501 acgtcctttt ttacattata aatgaccttg cgcttgatgg aataaaaaga gcatgatcat
    25561 gctatacaca cctaccgacc atggtttcaa gaaaatcaca atctgcataa attccctaaa
    25621 agataatttc gtcaaaccag ctactagaca gatcaagtcg tctggcgcaa aggggagcag
    25681 caaggtaacg actagtactt ttttgatatc tttcgttttt cttgtcagca attcatcaaa
    25741 tttctgatac ttttttggcg ataaaataag ctggacaaag gctacgccat agtaacggac
    25801 aagtaaaaat ccgattgctt ccccaatcac taggccaata caactatata aaagcccaaa
    25861 tccattgcca aacatcagca tgcccgctac tgaggagatc cctcccggca aaataggaat
    25921 gatcacttgg atcacttgca gcagaacaaa tatcatcact gcatacttgc caaattgctg
    25981 gataaacact tgcaaagacg tgctcgatcg aaaaatccca tgatgataag cataaagaat
    26041 caacacaaag aaaaacaaca gaccaataat gggcatgatc tgaagacatt tttgtaaaac
    26101 tgatcctttt ttagacatct atattcccca catctttttt catttattaa aactgcctcc
    26161 tt
//
LOCUS       DACEKH010000032        25933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEKH010000032 DACEKH010000000
VERSION     DACEKH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.032"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW662_001233"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW662_001233"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0866068.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IW662_001234"
     CDS             complement(937..1518)
                     /locus_tag="IW662_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0866069.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW662_001235"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW662_001235"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ0866070.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IW662_001236"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IW662_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ0866071.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IW662_001237"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IW662_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ0866072.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IW662_001238"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IW662_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0866073.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IW662_001239"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IW662_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0866074.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW662_001240"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW662_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ0866075.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IW662_001241"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IW662_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ0866076.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IW662_001242"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IW662_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ0866077.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IW662_001243"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IW662_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ0866078.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IW662_001244"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IW662_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ0866079.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IW662_001245"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IW662_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ0866080.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IW662_001246"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IW662_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ0866081.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IW662_001247"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IW662_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ0866082.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IW662_001248"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IW662_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ0866083.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IW662_001249"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IW662_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ0866084.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW662_001250"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW662_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ0866085.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IW662_001251"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IW662_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ0866086.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IW662_001252"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IW662_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ0866087.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IW662_001253"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IW662_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ0866088.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IW662_001254"
     CDS             13972..14157
                     /locus_tag="IW662_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ0866089.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IW662_001255"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IW662_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ0866090.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IW662_001256"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IW662_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ0866091.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IW662_001257"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IW662_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ0866092.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IW662_001258"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IW662_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ0866093.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IW662_001259"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IW662_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ0866094.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IW662_001260"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IW662_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ0866095.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IW662_001261"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IW662_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ0866096.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IW662_001262"
     CDS             18232..18879
                     /locus_tag="IW662_001262"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ0866097.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IW662_001263"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IW662_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ0866098.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW662_001264"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW662_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ0866099.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IW662_001265"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IW662_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ0866100.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IW662_001266"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IW662_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ0866101.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IW662_001267"
     CDS             20317..21255
                     /locus_tag="IW662_001267"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ0866102.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IW662_001268"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IW662_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ0866103.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IW662_001269"
     CDS             21935..22774
                     /locus_tag="IW662_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0866104.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IW662_001270"
     CDS             22750..23619
                     /locus_tag="IW662_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0866105.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IW662_001271"
     CDS             23609..24406
                     /locus_tag="IW662_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0866106.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IW662_001272"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IW662_001272"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0866107.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         8413 a         4777 c         5584 g         7159 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg
    25501 catcaagtaa taagaacaac caaacaaaaa tagctcgtca tatttttcgt ttggttgggc
    25561 ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaatcagcgt
    25621 tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt gccgtttgtt
    25681 gagctaatac cgaaggtcag ctgattgaac accgtttaag aaagaggtcg tgagcctgtc
    25741 atttaactct gagtcacaag taacaatttc aaaaaacagc ttctcatctt tgaggaatat
    25801 cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta ttttttctgt
    25861 aaccaaaaaa cgatggaacg ttgttttgat tgcccttaat gaggacagtc ggaacaggtt
    25921 tgtcattttt ata
//
LOCUS       DACEKH010000033        24801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEKH010000033 DACEKH010000000
VERSION     DACEKH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.033"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(64..1485)
                     /locus_tag="IW662_001273"
     CDS             complement(64..1485)
                     /locus_tag="IW662_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0866108.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1728..3605)
                     /gene="tdc"
                     /locus_tag="IW662_001274"
     CDS             complement(1728..3605)
                     /gene="tdc"
                     /locus_tag="IW662_001274"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0866109.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3896..5152)
                     /locus_tag="IW662_001275"
     CDS             complement(3896..5152)
                     /locus_tag="IW662_001275"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ0866110.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            5732..7033
                     /locus_tag="IW662_001276"
     CDS             5732..7033
                     /locus_tag="IW662_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0866111.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7102..7701)
                     /locus_tag="IW662_001277"
     CDS             complement(7102..7701)
                     /locus_tag="IW662_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0866112.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            7955..8836
                     /locus_tag="IW662_001278"
     CDS             7955..8836
                     /locus_tag="IW662_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866113.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            8849..10459
                     /locus_tag="IW662_001279"
     CDS             8849..10459
                     /locus_tag="IW662_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ0866114.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWKPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(10536..11195)
                     /locus_tag="IW662_001280"
     CDS             complement(10536..11195)
                     /locus_tag="IW662_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0866115.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(11484..12872)
                     /gene="ltrA"
                     /locus_tag="IW662_001281"
     CDS             complement(11484..12872)
                     /gene="ltrA"
                     /locus_tag="IW662_001281"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0866116.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(13377..14042)
                     /locus_tag="IW662_001282"
     CDS             complement(13377..14042)
                     /locus_tag="IW662_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866117.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(14189..14794)
                     /gene="dhaL"
                     /locus_tag="IW662_001283"
     CDS             complement(14189..14794)
                     /gene="dhaL"
                     /locus_tag="IW662_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ0866118.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14822..15805)
                     /gene="dhaK"
                     /locus_tag="IW662_001284"
     CDS             complement(14822..15805)
                     /gene="dhaK"
                     /locus_tag="IW662_001284"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ0866119.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15807..16202)
                     /gene="dhaM"
                     /locus_tag="IW662_001285"
     CDS             complement(15807..16202)
                     /gene="dhaM"
                     /locus_tag="IW662_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ0866120.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            16356..16676
                     /locus_tag="IW662_001286"
     CDS             16356..16676
                     /locus_tag="IW662_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ0866121.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16788..17657)
                     /locus_tag="IW662_001287"
     CDS             complement(16788..17657)
                     /locus_tag="IW662_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0866122.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17753..19057)
                     /locus_tag="IW662_001288"
     CDS             complement(17753..19057)
                     /locus_tag="IW662_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0866123.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(19054..19563)
                     /locus_tag="IW662_001289"
     CDS             complement(19054..19563)
                     /locus_tag="IW662_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866124.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19738..21872)
                     /locus_tag="IW662_001290"
                     /pseudo
     CDS             complement(19738..21872)
                     /locus_tag="IW662_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(21955..23610)
                     /locus_tag="IW662_001291"
     CDS             complement(21955..23610)
                     /locus_tag="IW662_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0866125.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23612..23926)
                     /locus_tag="IW662_001292"
     CDS             complement(23612..23926)
                     /locus_tag="IW662_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0866126.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            24096..24356
                     /locus_tag="IW662_001293"
     CDS             24096..24356
                     /locus_tag="IW662_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ0866127.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            24370..24684
                     /locus_tag="IW662_001294"
     CDS             24370..24684
                     /locus_tag="IW662_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ0866128.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         6888 a         5169 c         4475 g         8269 t
ORIGIN      
        1 tattcacttg gcaggacctc ctactaaatc agcgacaaaa cgtcggctga aagacttatt
       61 tttttatttt tcttctaaac ttttttctac ctcaatcccc caagaatctt tcttgaagtg
      121 attgatgatc aatggaataa cgaacatgac cactgtgata gctgctacta ataatgcatc
      181 actcaatgtc gctgtaccta atgtagcaaa tacgacaacg atgatagaga agatcagcat
      241 gcagctgact acccaagcta atccgtttcc gtttttacca attcggtaag gacgttcgaa
      301 ttctggatgt ttcttacgca ttttaagcaa tgaaacagca atcaaaatgt acacgataca
      361 ataaataaca gttgtagcat ttgtaagtgt caagaataca gcacttacgt catccattac
      421 accataaagc aaagcaaata atgaaataac gattgattgt gttagaacga catttctaga
      481 tacgccgtat ttgttttctt tatggaatcc gaattttggt ggaaggaatc cttcacgagc
      541 tacttggatg attgtttttg atggtccagt aacccaagct gataattgta aaagcacacc
      601 aataaatacc atgaagctga agatatttcc gatcacttct ggtaagccta ggatttgaca
      661 gtcaatcaag ataggctgcg tgatatttgc taattccatt tttccatctg gtacgacatt
      721 tgctacaagc atcgcattga tgacattcaa taaaactaat ccgatcaaag cgataaatac
      781 gccttttgta taattttttg ttgcgtcttt caaacgaggc atgtaaacag aactcatttc
      841 gatcccaaca aaaataaatg tgataccagc taagtatttc aatgaatcaa tatgttccaa
      901 gtcagggaaa gcttttgacc atgaaaagtc acctagatag ccgtttgaga ctagtccgac
      961 tttgaaggct gccaaaacac ctaaaacgaa catgatgact actgggatat atacccctag
     1021 ccatacaccg atgttcccgc cgacttttgc catatcgaat ttcaagttca agatcgtgat
     1081 gatccagtag ataactaaaa tacaacctaa aacgaaccag tgattattgc caagttctac
     1141 attaccaaat gtgtttccta acaatggtcc taaagtagaa gcgaccatta ccatacctgg
     1201 gaacatttgc acccataata gccatgcaac gacaaatccc catttgctgc ccagtgctgt
     1261 ttttacccat aactgaggac cgccttcttg aggaagcatc gtggaaagct caccggaaac
     1321 cattgatatc ggaccggcaa aaaacaaaac tgcaaaaatt gaataaaaaa tcaatgtcca
     1381 accgactgcg gctaacgtag gaatactccg aaccgttgca cacagtgcca ttgtcagacc
     1441 gataaaggtc ccaagaccca ttttacttac ttcagattta ctcaccgttg acactctcct
     1501 tgattttttg gataaaataa cggtgtcttt gaagtagttt cagcaataaa cgaaacaatt
     1561 cagcgaatat ttgaggggat tctttgagtt tattgcttga agggtttctt caaagacaac
     1621 ctcaaaacaa agggatcgaa gagcttaggt ctccccttgc tcttcagatc caaacatgcg
     1681 tcagaaacag ttagtattca agctgttttc taacatgatt accttgctta ttttgcttcg
     1741 cttgccaata attggtctgc gtagattttt tccaattttt cttgaagagc tgctttgatt
     1801 ttttcagcat attcttcaaa gtgttcttct ttgttcatgt atggtgtcat tacgctggca
     1861 cgcaatacag ttacttttcc ggcacggttc cattcttcat ctgagaatcc tagttggttc
     1921 acaaattgta atgggctgtt gccatagtct gggatagcaa agtcagtatg tgaagttaag
     1981 aactcattac cgtaaatgct tcctttcacg tatgaagaat aatcatatac gtcatggttc
     2041 aatttattca ttgcaactaa gtcatcgttg cctttttctt tgaatacata gtctaccata
     2101 ttgaaatctg gataagttaa tggatgaact tcgatttctt tgtctcctac tttaaatgac
     2161 aagtcattca agaagttgta gaaacgatgt gcaccttcga ttgatgcacc cattaattta
     2221 ccatatcctg tgacattcaa aggtaagaca tggtgtgctg cccaaacgct ggctgctgtt
     2281 gcccctgctt ttgaaccttc caagatataa gccccaagta atgctgggat atctgcgcct
     2341 ttttcaaata catatgtcgc aaagtaagag ataacgtctc tcatgcggat atctttgatg
     2401 acgataccac cggcagaata tggaacatag cccattttat gtgggtcaat cgttacagat
     2461 tctgcttctt cgattgcttt atatgcactg tgtacttctt ctaagatata atctttgttt
     2521 tctgtaaaga cattgtattt gtaatgaaca tcttttaaat cttcaaatgg aatgaagttg
     2581 ttgtcttcgt ctaagaaaat cgcacggccg tatccgccat aagcagcatc tacgtgtaag
     2641 tagaagtaaa taccgtcttt ttctaatacg cgacgcaatg caacgatttt atcgataccg
     2701 tcgattgctc cttcttctgt tgatcctaca acaccgacta cacctaggat tggtgttttt
     2761 tcagctgcta gaccacgaac gattttttca agttcgttga tgtccatgcg atagttgtgg
     2821 tcaactggca ctggaatcac ttggtctaaa ccgataccga tgatgtcggc agcttttagc
     2881 catgaatagt gtttagtttg tggaaccaac cattttccta atttttctaa atgttttccg
     2941 ctacgtgctg agtgagcttt gatttcgtcg attttttctg ggacgctgtc taacagattc
     3001 atgatttctt ctgttgacaa gttcattaat tcccaatcgc tttttccagc aactagttca
     3061 ggtgtcactt ctttcattgc aagcggcaat gatttgatgt tgcgggcata ccaaagtcct
     3121 tctaagtttg ctaaagaacc atcagcaacg atgtggcccc agccatcttt atagctcatt
     3181 aattttgcaa attccattcc tacttcttct tccatttggc ttgttgcagg agaagattca
     3241 taggcaacgt tattaccatt ccatagcata gcaaaattgt acgctagtaa agatggcatc
     3301 aatgtttcag agttcatatg accccagtaa cgtccggcat tatgccaagg aacggaatgg
     3361 gtacgcatcc gcgctgaaat ttctgataga acatcctttg ttttattcac agtgtgttca
     3421 aaactagcac tgcttttttc ttctggcgta ataataggca tgtcttgagg catgtagttt
     3481 tgacgccagc ctaaatgttc atccaccaat tcattcaaca aagctttata gatttggccg
     3541 ttctcagctt tgtccccaat gaatagggca tttaggttta gatctttcga caatgattca
     3601 ctcatagtga aaccctccta aaaaaatatt taaattttgt cgagccctac attacacctg
     3661 ttcttctatt ctgtaaacta agttttggcc tgatttttaa tcatatttta atatgtatca
     3721 attatccgaa ttgttcacac ttttgaaata attaaaaaaa acgagggaat ttacaaatgt
     3781 aaactccctc gcaaagattg tattaacgac aaccagcgtt ctattttagg atgggtttca
     3841 aaattggaat gttgtaatag tatttccctt tttcaaggta aaaacttact aaattctaat
     3901 caattacttt ggccaaaaag taatttttct taccttttcg aataaccaca aacttaccat
     3961 cgaattcatc ggatggattt atgacaaaat ttaaatcggt aacgcggtca ccgttaatac
     4021 ttatagctcc gtttgaaaca tcttcacgtg cttgacgttt agatggttca atttttgtgg
     4081 aaacaagcag ttccacgata ttttcaggtg tgcttgaaat ttcaacgttc ggcattttac
     4141 cgaatccttg cgcgatttct tccgcattta gttctttaat atttccagag aacaatgctt
     4201 gcgttatttt ttgcgcttct ttcaagtctt cttcactatg cacaaatctt gtaacttctt
     4261 ctgccaaacg gcgttgtgct tcacgtttct caggttctgt ctctactttt ttcgctaagt
     4321 cttcgatttc ttcttgagac aagaaagtaa agaatttcaa gtatttcact acatcgcgat
     4381 catcttgatt caaccagaat tggtagaact caaatggtga agttttcttc ggatcaagcc
     4441 aaacggcacc gcctgcagtt tttccgaatt tcgttccgtc agccttcagc atcaatggga
     4501 tcgttaaacc gaacgcttct gcttctggtc cttctaattt tcggattagg tctaatccag
     4561 ctgtgatatt tccccattgg tctgctccac cgatttgcaa ttgcacatcg tatgttttat
     4621 gcagatgcaa aaagtcgata gattgcagga tttggtaagt aaactctgta aatgagattc
     4681 ctacttccaa acggctggca acgatatctt ttgctaacat cgtgttgatg ttgaagtttt
     4741 taccgtagtc tctcaaaaat tcaagtaaag agatttttga caaccagtcg tagttgttca
     4801 caaatgtgat atttgcatct ttaccaaaca attttctcat ttgattggaa agtgcgtcca
     4861 cgttatgttg cacttgttcc atcgtttgca acacacgttc tgttttgcgg ccgcttgggt
     4921 cgccgatcgt tcctgttccg ccgccaatca gaatatatgg atgatggcct gccaattgga
     4981 atcttttcat catcataaat ggaatcaaat gtccgatatg catcgaatca ccagttggat
     5041 caacgccgca ataaagtgaa atgctctttt cttcaacgag ctcgcgaagt ctttcttcgt
     5101 ttgtttgctg attaatggcg tcgcgccaag ttaattcatc aataatattc attttactga
     5161 cctccgtgta gtttttttat gaaagcagga acctgctagc atgtgtaatt aattatttct
     5221 tcaggatagg ataggaaata aaaaaagtcc cttggcatat aagccaaggg acgaaatctc
     5281 gcggtaccac ccaatttgta tgtctagata tccaacatac ctctcgaaca tttaacgctg
     5341 tcgcgtggca atcactgcca aagactcctg aaatgtaatt cgctttaaag catgtactgg
     5401 tttgcagcga ccaccagctt tctgaaacat tggctttaac aactacttcg tttcgatctt
     5461 cgtcttgtta ttattaatat gtgtctatca tagagcgcaa ctttttcttt gtcaacagaa
     5521 aaaggatatt ttttttaaat tcctcatttt ttcttttttg aatccgctct aattccgaac
     5581 attttatttt caattattgt taaaagaaat aactttttct gtattttttc tgcaatttat
     5641 cgagaagtca ctttactttg cctcttattc tttcttcgtt acaataagga atgggacaag
     5701 taaaaaaatg aaaaagcaag ggacgaggta tatggaaaaa aagcaaaaaa ttattttagg
     5761 agccacttct gctttaacta tcgttatttt aattctaagt ttgttttttg tcatccgatc
     5821 atctacaaaa aaagaaccaa tcattcctga aaaaaaaata acagaaaaag caaaagaacc
     5881 gacagatgat aagcaacaac tagttactca agcaaaaaat ctggctcaaa gctacgatta
     5941 tgaccaagcg attgcgttat tggaaaaaga tagtaagaac ggatctactg aaacacaaca
     6001 gcttcttcaa acactaaaaa aggagaaaga acaactgatc gactggtctg atcctactca
     6061 gatctctcat gtctttttcc acagcttgat cgtggacccg caaaaagcct tccactctca
     6121 acaggcacaa ggatacaaag attatatggt gactgttgaa gaattcaacc gttcgatcga
     6181 acaactttat caaaatgatt ttgttttagt taatttgaat gatttgatcc aaaaagacga
     6241 acaaggaaat ctttccttta acgggcttgc gctacctgaa ggaaaaaaac cattggttct
     6301 ttcacaagat gatgtcagct attatgaata tatggataat tccggttttc ctagccgatt
     6361 ggtcgtatca aataacaatg aaatcaaaaa tgtctataaa gagaaagata aagaagaaat
     6421 cggtgacttt gacatggttc ctctgatcga tgcctttgtc aagaaacatc ctgatttttc
     6481 ttatcgaggc gcaaaaggta ccttggccct gacaggttac aacggagttt taggttatcg
     6541 aacatcaaaa tctcaatatg gagacaatga aaaaacaaaa aaagagatta aagaagctaa
     6601 aaaagtggcg gaacagttga agaaagacgg ctggagcttt gcttcccata catggggtca
     6661 cttgaatatg actcaagctt cactagaaga tgtaaaaaaa gacaatgaat tgtggcagca
     6721 agaagttgca cctatcctag gaaaaacaaa tattctgatt tatccatttg gtgcagacat
     6781 cagcgactgg cagccttatt cagcagaaaa tcaaaaattt gcttacttga agagtcaagg
     6841 tttcgatatt ttctgtaatg tcgatgcttc tacaccagct tggggacaat taggtacgga
     6901 tttctatcga aatgctcgga tcaatatcga tggcattcgt tttcaatctg atttagacgg
     6961 aaaaaatcct attcttgatc cattcatcaa tgtccatgat gtctatgaca aagaagcacg
     7021 cgaatctgct tgattttacg tcatttatga accaaaaaag ccgaactgac attttatcgt
     7081 cagtttcggc ttttttatgc tttatttctt cttcggtgcg attcccttga tcaggacttc
     7141 tgtccattct gctgtatatg gaagcagttt tgcgtatatc tccggcttag aaattccttc
     7201 taccattgct tgaaaataga ggatcaagaa ctcatccgaa tatttcaaat ccacttttcc
     7261 ttctttccgc cctcgatgga aaagatcaag catgatactg aaactttctt gcgtatacgc
     7321 atccatcgcg tcagaaaatt cttttttttc tttttttgta tagtatgtca tcaaatcgat
     7381 ataaaattgt ttgttcactc gattgagata acggattttg ttttcactca tggcaatcag
     7441 tgtttcttca aatggaagat tttccgcaat caacgctttc gcttcttgcc ctaaatcatt
     7501 cataaatgca aaaaatactt cataaatcag cccttccttg ctgccaaaat atttaaaaat
     7561 cgtcgcttta cttacctgag cgttctcagc taccttatcc attgtcactc cagcggttcc
     7621 tatgtctgtg ttcatcaagt caaaagctgc ttgtaaaatc tctttctttt tctcttgtgc
     7681 tctcttttca aaaccgttca tttgtagagc ctcccgtaat tttaaacttt tataatatat
     7741 atagttcaaa tttattctga ggtttattat aactatctat tgagaaagta tcaagctagc
     7801 aattccttgt ttatgaattc attgacagaa atttataaaa agaatattat gtactaaaat
     7861 aataaaaaaa gttttataaa aaggtataaa acctttttat taaactattt aatcttgttt
     7921 agtatatatt tttagaaaaa ggagtggggt ttttatggca gaaatcgtca aagtcacaca
     7981 tttgaaaaag agctttggtc gtttcgaagc tttaaaagat gtctctttca ccgtacattc
     8041 aggtgaagtc gttggattta tcggaccaaa tggttctgga aaatccacga cgattcggac
     8101 acttttaggg attataaaaa aagacggcgg aacggcaacg atttttgggc aagatgtatg
     8161 ggaagatagt ttatctatcc atgaacgtct ttcttatgtt ccaggcgata tctctttgtg
     8221 gggaaatttg actggaggag cgatcatcga tctcttcatg aagcttcatg gaaaagggag
     8281 tgctcaaaaa aaagatgaac tgattcaacg tttctctctt gatcctaaga aaaaagcaaa
     8341 aaactattca aaaggaaatc gccaaaaagt gggcttgatt gctgctttgg ctgttgattc
     8401 ggatctctat ttatttgatg agcctacttc tggtttagat cctttgatgg aaacgatttt
     8461 ccaagaagaa gttgaaaaat tgaaagaaca gggcaaatcc atcctacttt cttctcatat
     8521 tctcagtgaa gtcgagcgac tagcggataa agtcgtgatc atacgcgaag gaacactcga
     8581 tgaagaagga acactcgatg aattgcgtca tttgactcgt tcgaccatca ctatagaaac
     8641 agaagcagag aatagtcact tagcacaatt gaacggtgtc catgactttg tgcaaaaagg
     8701 tcacctcgct caatttgctg ctgataacga agctttcgat gtgattttag ctgaagcgtc
     8761 caaattaggt gtgaaaaaat ttgaatctat tccaccgact cttgaggatt tattcatgag
     8821 acattatgaa ggatgaggag gaagcgaaat gactgataaa ttttcacgct ggtccaccct
     8881 tttttctcaa aaccttagcc gtgactggaa aaagatcctg atatggctag tcggtgtcac
     8941 tgctttttct ggtggttttg tccctgcgtt tgaagaattg acttccggac aaggattagc
     9001 tggattatac gaaaccttgc aaaacccggc aatgattgca atggtcggac caactccagc
     9061 ttcgaatgct gctgagtata ctttaggagc gatgtatgca aatgaaatgt tgcttttttg
     9121 cagtttgttt gctgctgtga tctctgtttt acacgtggtg agccaaacga gaaaaaaaga
     9181 agaacttggt ctgattgaac ttattcgttc ctttcgagtc ggacgtcaag caaactcttt
     9241 agctgtcatc gctgaaagta ttctgatcaa tttgctttta gcaatatcga tcagttgtac
     9301 gatgattttt ttccaagcgg attcgatcac tgcagaaggt gcttggcttt ttgggctatc
     9361 tgtaggtagt atggggattt tggggagtgc cgttgcactg tttgctgcac aactgatgcc
     9421 aagttcagcc agttcgaatg gtctgtcttt agcgttttta ggtggacttt accttctacg
     9481 agcaataaca gatgtagctg attcctccct ttcgatgtgg aaacctttag gctggtctta
     9541 tttgacctat ccatttacaa ctaacaactg gattcctttg ctttatttgt ttttgttcag
     9601 tctaatcttg ataatcagtt catttgtttt agaggaacat cgagatttga acaccggcta
     9661 tatccccgaa cgtgaaggaa aaccgtatgc taaacgttct cttttatcct tgcgtggttt
     9721 attattctat ctaaataaag ggatgacaat cagttggctt atcacttttg gtttgatggg
     9781 ggcggcatac ggctctatct acgggaatat gcaacagttt ttagaaagta acgacttaat
     9841 gaaacagatg tttatccaat ctggcgtctc aattgaaaaa aactttacaa gcacgatcat
     9901 ggtcgtcttg atttgtctag ctgctatttt accaattgct agcatcaatc gtttatttac
     9961 agaagaaacc cacggacgta tgaatcagct gttcgcaaca aaaatttcta ggaaccaact
    10021 ttattggacc aatatgttac tagctattct atttgctata ttggcaatcc tgattagttc
    10081 tggtgcacta gggctttctg ctttggcttc gatgaaggat gcgaaagaca tggatctatt
    10141 gatgtttctt aaagccgggt tcaatttcct gcctgccgtt ttagtgttta tcggaattag
    10201 tggctttcta ttcggatggc ttcctcgaat cggtaaactt gcttatatct acttagctta
    10261 ctcctttgca ctcaattact ttggcggaat tttggattta ccagaatggt tttccaaaac
    10321 agctgtccct agctgggttc cacggatgcc aaacgattct tttgatcttt ccgtgttcct
    10381 tacgttatca tgtatcagcc ttgcactgtt cgtccttggt tctattggtt ataggcgaag
    10441 agacttacta gaaggataat aaaaaagcat gtacaatttc atgctagcct ccaaatctgg
    10501 gtcaaaaatc caacatttgg aggtcatttt tttatttaca agtcttctaa ctgctcttta
    10561 tcaatgatcc agacttctcg attctttctt tttacataac cattctcctc taataattta
    10621 aattttctag ataaagtttc gggggtcgta ccgataaatg cagcaagttc tttcattttc
    10681 attggcagga aaaagtgatc cttccctgtc gctttagcta agtcaagcag atagacagcg
    10741 actcgttctt ctactttttc catcatcaaa aaagttgtct gctgctgtac ttttgttaat
    10801 ttctcaacgt tgattgctaa aagtttcaga ctaagggcgg gataatgcag taagagttcc
    10861 gagaaatctt tttgtctcaa tatgcaaatt tccgtttttt ctagtgcttc gccaaataaa
    10921 gactcatttg gaacgcctag caactggctc tctccctcat atccgcccgg ctctattact
    10981 cgtaaaagct gttcctttcc gctagcagat aattgataca ctttcatact gccggaggcc
    11041 acgatcacta attgaggttc tccattcggc gcaagaatct gttctccttt ttccacatag
    11101 tgatgtttga caagtgcgtg tactttttct tgatcttgct gtactaagtg attgaacaaa
    11161 ggaacaaatg aaacacataa atgatcatga ctcataatct ctcctccttc taaacagcta
    11221 tttctaataa agaaacataa cttcccttat tccaactgat aattttcttt tatcatagca
    11281 taaacgtcaa gcgaaccaga taccagcaaa tagagtggat ttctttttgt cccttgcttt
    11341 attttccttc ttttgagacc ttaagtaatt gattcctatc aattttttaa atatttactt
    11401 tttgaataat cgagtagagg ggaaagccaa ctgaccttcc gcccctctca caccaccgta
    11461 cgtacggacc cgtatacggc ggttcaataa gttgagtgta cgaactggta cttcaaggag
    11521 atgtcctcat atccttggcg ggccagttct ttgtctgtta atgatcgatg aagaatatga
    11581 cttttggaga ctgaccaaat accttttctt gtatttgacc attcatacgc tttcgcttta
    11641 tcgatcccta aacgaatcag attttttctt ttggttcttg gattcttcca ttgtttccaa
    11701 atatattgtc tgattcttcg cttcaaccaa ccgtttaact ccctcatgaa ttctttcatc
    11761 tcaccaattc catagtagtt gatccaacca gtcattagtt gcttgatttc ttttaaaata
    11821 agttggatac ttcttccacg gtttctttta gtgagttgtt tgagcttgct tttcacacgc
    11881 tgttttgctt ttgcgtgagg gcgaatttta atacctttct ttgttggcag aatgcagaat
    11941 ccaagaaact ttcgtttcaa tggacttcct accgcactct tttctcgatt taccgtcaac
    12001 tttagttgtt cttcgagaaa ttgaatggat ttcttcatca cacgctgtcc tgctcgtttg
    12061 cttctaacat agatattgca gtcatcggcg tatcgaacaa atttatgtcc tagattttct
    12121 agcacttgat caaattcgtt caggtagatg ttacttaaca ggggagagag attgcctcct
    12181 tggggcgttc cttcttcagt tgttgtgatg agtccatttt ccattacacc actcattaag
    12241 tattgacgga tcagttttag gacttgtttg tcctttactt tccgctcaat gaaatacatc
    12301 agcttatcgt gatttacggt gtcaaagtag gctttcatat caatgtccac cacatatcgg
    12361 taaccttgtt cataataaga ttttgctttc ttcactgcgt catgtgcgct gcgatttggg
    12421 cgaaacccat aactattatc tgagaattct ggttcaaata gcggttgtaa cacttgattg
    12481 attgcctgtt gaagcatacg atcaataacc gtgggaattc ctagttttct tacaccgccg
    12541 tttggtttcg gtatttctac tcgcaaaaca ggctgaggtt tatagttccc ttggcgtaaa
    12601 ttggatagta attcctcgcg gtgctctttc aagtaaggaa gcagttgatc gacggtcatc
    12661 ccatctgcac cagccgcccc tttatttctg actacttgaa gatacgcttg attgagatta
    12721 ttccgatcta gaattctccc aaacagattt tctacaccat cttgtttcct ctgttcaccg
    12781 caagaagtgc tgcgcacttc atcataccct tcgtgttcca cactagctct ttgatgatag
    12841 ccaagttttc ttgttttctg cggtcgtcgc atcttctcac acctcctaaa ttattcaaga
    12901 ttcttattgt tcagtccttc gtaacggtca gtgtcgttac tactatgacc tcggctgact
    12961 tctcatgatt caccatcatg tcgccatgat cgttgtgctg gtttgttttc attcaacgcc
    13021 cacaacaccc tcatgagatc tcccccggta agagtgaaaa ctttcctccc atgtaactgc
    13081 tagatttact gtataggttt cgggcagtat tggactttgt cttgtgatga agacttatcc
    13141 gacctaattc agccttgtat ctagtttctg ttcgtcagtt caggagtttg cgtccgactt
    13201 ccttcagcca caacctcacg attgccgcct tgtctttcgc taacagttcc tactgccaag
    13261 cctgtagtgg actttcacca ccaagttttc acccatgcag ggcgcacatc aaaaacaaac
    13321 cttcactcgc aagccgagag taaggtttgt ttttcgcatt gcttatttcg tgttgtctat
    13381 tgaaattttt ccgcacctag cttttgatat agtggtgcta gttcttgtaa ggtttcagcg
    13441 gttatttgct caacgtgttc gatttcttgc ggatagaaga atagtttacc catttgaaag
    13501 gttcggtctc tcatcgcttt tttcaactct tcttgtttct ttgtatcgga caaatagtca
    13561 gacaaagcta agtggtcgtc aagttcatgt tctggttgcg gccatatgta ataatccttg
    13621 atatcgactg ttttgctcca ctctttcagt atcgcaagca actggttata cgctgcaatg
    13681 ctaccatatc gttcttcgct tttataatgt tggtacatca aatagatttg gtattgtttt
    13741 tgattcaaca aaactgctag acaggcattc tcgtcttgac gagattcgct tcggtacgcg
    13801 ctccaaaaat gactgcgtaa attccagcca ttcgtccagc tttcgatttt aggtttgctc
    13861 atgccgtaca tatcaggcaa aagtgcagct gtctggagct gaatctcttt ccatttttgc
    13921 cagtgttctt tatattcttt cttgatttgt tcaatcttat ctgttggatg gctctctttg
    13981 agctgtttga atgcgtagat tggtcgatca aacaactgat aatctgattt tccaagttcc
    14041 attgtttcgc ctctttccgt tatcttttgt attctacctg aattttgcga tatggacaaa
    14101 tagaggttgt gaaaaagctg ttctgcatca agtcataaga acagccaaac aaaaaactgc
    14161 gatgagcatc accgcagttt tattgaactt aaggttgatt gatcacatct aaaagcgttg
    14221 aaaataaaat ggcactagaa gcagctcctg ggtcgatatg tccgattgca cgctctccta
    14281 gataggaagc tcgtcctttt ttagctacca agactttggt gtgttctttt gcctggtcaa
    14341 tgatttcttt tgttaaattc cctgccttca atgcctctgt gactggcagc caaacatcta
    14401 acatcgtttt atcgtcagct tctgcctttc ctcggctttg aatacccgct gttccctgct
    14461 cgatcacttc gcctaatttt tcttgagaat caatagaatc gacctctttt gtcgctttca
    14521 tcatgttcat gaatgcagat ccgtataacg gaccagaagc cccaccgact ttcgatatca
    14581 tcgccataga gaatactttg aatgtatcgc tgattgtttc aggatgatcg gcttgaaaag
    14641 cttcttcata agctgaaacc cctcttccca tgttatttcc atggtctcca tcgccaatcg
    14701 gggtatctag atcactgaga tattgtttgt tctgattgat tgcctctgca aattgcgtga
    14761 gccattcttg gatttcttga acggttagtt tcatattatc acccttttat cttgagattt
    14821 tttaccatga aatcgtctgt acattgcttt ctaaagaatc tttccattgt gtctccgtca
    14881 agtcgatcag tgttagagaa agaccttgca tatctagtga ggtcataaaa ttaccgactt
    14941 ttcggaaaac tacattgacg cctctttctt ccaacagagc taaaacatca ttcatgaaca
    15001 cgaactgttc catcaaaggc gtcccgccca ttccgttgac tagtacacct gcttcttttg
    15061 gtaaattcga gtaatcatct aaaatcttag taacaagttc ctttgccagt actttagaag
    15121 gctgcatctt ttctcgacga tagcctggct caccgtggat accaacacca tactcgatct
    15181 catcttctcc tagttcgaat cctggtttcc ctacttctgg tacagttgct gctcgaagcg
    15241 ctagaccgat cgtcttagtt gcctgtacga ccttttctcc cagttctttc aactcagcta
    15301 gggaagcacc gtttcttgct gcatcgccca cgattttgtg tacaaggaca gtacctgcaa
    15361 ctccgcgttt tccagcagta tacgtactat tttcaacggc gatatcatca tctacgatca
    15421 cactttctac ttcaatatcg tccatagcag ctaattcctt tgccatatcg aagttcaatg
    15481 catcccctgt ataattctta acgatcagca aaactccttt tccactatcg gcttccttaa
    15541 tagccgtctg aatctgatct ggagttggag aagtaaacac atcaccaagc accgctgcac
    15601 ttaacatccc ttcccctaca aacccggcat gggatggttc atgtccgctt cctccaccag
    15661 aaaccaaacc aacttgttcc gctttctgat tcttggctac aacacgggaa gattctacac
    15721 gatgaacaag ttctggatag ctttttacta atccttccag catttcttcg acaatattgc
    15781 ttggatcatt catgattttt ttcaatttat tcccatcctt tctttccggc attttgtgct
    15841 tctgttaaga tttgctgagt atcatctgtt ataccggcag tgatggctgc tgcaaaagct
    15901 ccttccacta aaggcgcatc caccagatga taacgttgct gctgctcttc ttccaacatg
    15961 tcttttgcca gttccgagtt caataccgcg ctaccgatat ccgcaaaaac aaaataacta
    16021 gctgcatcgc ttgattcatt gactgcttct acgattttca tcgggtctga tccgatctcg
    16081 ccatttattc cgccgagaga aaaaatttgc acctcttcgg atttttgcat ctgctcaatc
    16141 atttctttaa taccatctgt tactttttga ctatgagaaa taagcaaaat actttttttc
    16201 acaagaatct tcctttcttt tacatagttg ctaacgattg gtttcgcttt cattaatgaa
    16261 caattcaagt atataataaa tttctaattt tgggaaaata tgtgtagtgc ttcttgaagt
    16321 ttgttatact ttaattaaaa gagaggagtg atcgaatgat tacaacaaca acgaacagtg
    16381 tagagggaaa agccgtagta gcttataaag ggatcgtttt tggtgaggtc attactggga
    16441 tccatgcatt taaagacttg gaggcggggt tgcgaaatat ctttggcggc cgctcaaaaa
    16501 gctatgaaaa cgaactaatg ggtgcaagag aagaagcact tgccgaaatg gccgaacgtg
    16561 caaaagcatt aggagctgat gcgatcattg gcgtaaaaat ggactatgaa gtattaggca
    16621 gcgacaatgg catgctgatg gtcacatgta gtggaacagc tgtctccacg agataagtag
    16681 gggacatctg ttttaataac taaagagaga tgggacatgt agtggcactc aagaattaga
    16741 tttttcagtc taacttcgag gtactctacc ttcccatcct ctctttttta tttctttttg
    16801 cggatgattc cgtaagctgc tgcgactaac acacccgccc ctgctaagga ataactgaca
    16861 gaagctgttt tttcatttgt ttttggcaaa gtctgacttg ttgatttgga tggctctgtc
    16921 gtttgctgaa ctgtttctgt cgtgcttcca gaggtcactg atttttctcc tgccgaatgc
    16981 aaaatatctt ttattcgttg gaaactagct gctaagtcag ctttttttgc ttcaaaatca
    17041 ttagcaactg tttgtgcttc gctgatcttt ccgtggattg cttgactggc cgctgtgata
    17101 tccgcttgag tttgacttag ctttccagaa gctgtttgag caacctgatt taattgctga
    17161 ttgaattctt ttgcttgttg gatcttcccg tcaagatctg tttggacagc ttccgctttt
    17221 gctttcgctt cggcaagctt tttctcaagt tgtgcacggt catttcccac tttattttta
    17281 agatcagcta aatcttgatc gattgctgct tggtcttctt tcaatttgtt ttttgcagct
    17341 tctaatgctt cttcgagttc ttttctttta ttttccaagt cttgcaaatg atcgatttgg
    17401 tcagaagagg tagaactatt tattgatgta tcttctgaag atgaaatcgt ttctcctgaa
    17461 ttatctgtcg gtacattgga agtcatttct gtttccgagg caccgttttc agcaccagaa
    17521 gagcttatgg ttgttgaagg ttctgtttga gcaggttcag aagtttccgc aaaagctgtc
    17581 tgtcccaata agaaaatact ggaaacagca attggccaga ttaacggctt cattcttttt
    17641 ttcatcactt ttcccatcct tactactttt taactaattt cttattataa gcaaaaatgt
    17701 ttggaagaac cataaacaca acaggctgtt tgtctaaaaa acggtgcatc ttttattttt
    17761 ttgtacgttc cgggcttttc catgtcccgc ttccataacc taaaatcgtt ttgactgctg
    17821 gaatgaaagt cgtcacgatc gttatcgcgt tcactatcca gaacaaaagc atatatagtg
    17881 gtgcgaaaag aaggtatttc aattttcttc gtcgatcatc tacaatcagc gaagctagca
    17941 actggaaaaa tcctgcgacc atttcaaagc agatgaacac aaaactgaat gtaaaagtaa
    18001 tataaatctg ttcgtagttt ccttgatata cataatgccc gattaatcca gcaaaaagaa
    18061 ctacggaaat acaaaagaat attgaccaga caatactcaa tgtttgatca atgaagatga
    18121 tcgttcttcc aatatgttta aaaggatgga gcatcacttt tttgaaattc gtcagccaga
    18181 cttctgttcc accttttgcc caacgttttc tgtgtcgata tagcatcttc aacgtcacag
    18241 gaacttccat gaaaaagata atctcaggag caaaaacaga taaccagtca tttaactgat
    18301 gatcccaagc aatactgatg tcttctgtcg ctcgatcttg tctgaatccg cccacatcga
    18361 tcaaggcttc ttttcgatac atcgtatttg ctccactgta cgcataaaga ccaccgaaaa
    18421 cagctgactg tgttcgttta atgatgccca cgatacttga gaattcaacc gtttgagatt
    18481 ttgcaatcaa tttggtacga ttttggacat ccatatttgc agtgaccgca gcaatatgtc
    18541 ttgcaccagg gcgaatgaaa taattcacat acctaatcag tgcatctggc tccggtaccg
    18601 tgtctgcatc attgcttaaa atcaatttcc cttttgcaaa ggcaagtccg atattaaacg
    18661 cgtgggcttt tcctttattt ttttcgatcc gaaccacacg aagattagcg tattttttca
    18721 tcagtctagc taatatttct ggtgtttggt ccgttgaacc atcgtctgtc actaatacct
    18781 catagttgtg ataattcaat ttcgtcatca aatactcgat tgtatattcg atcacaattt
    18841 cttcattatg ggctggcacc atgatcgtga tgaacggttc gacactcaag ggaacatcta
    18901 cccattcttt ttgcttatgt ttgtatataa acacataaca ccagacacca ataaaccata
    18961 caaatccgcc taaaatcgga taagaaacga gaaaaaaata cataatagac aaacaagtat
    19021 ctaagaataa attacctgta tgggtgattg agatcatttc agttccaccc cattttcttt
    19081 atacaaatct gcaatgaatg tcgtatctag atttttctct tcggagacag aataaaaacg
    19141 gaccgtttct ctctcttctt ttgttccaaa acgttccgta tacacttctt caagtaattc
    19201 ttgtcttttt ttcaattttt catcttcata ttcaaaagat ttatttactt ttttgtatag
    19261 atttcgattg ttccacctag tcaatacaaa tgaggtaatc aggaaactga aaaaaatcaa
    19321 tacaaaaaag ccgattaaga aatacagcat tttcttttct gataaaaaag cgtacaagga
    19381 aaaatccttg gctctttctg gtctcgttaa tgggaaaaag acccatataa aaggaagaac
    19441 aacgatcatc catcctaaaa gagccactaa cgtttgccaa atctttaacc agtaattacc
    19501 ttttgaaaaa tacttatctt tcaccaaaat tgatgcaact tcttctcttg ttactttttt
    19561 catatcctca cctcacgttt ttttgttacg acgtaggagt ttaccataca tttcatttct
    19621 tttcacataa agtgttccta aatttttcct aagaaaaaga acaaaacatt tctcatcagc
    19681 aaatgaaaaa tattttgttc tttttatcag ttgttctttt tattttttat ctcttaatta
    19741 tgttgctctt tccaatacga caatgctttt cattcctcta gatatcgccg tgtacatttg
    19801 tctgcgtctt ctttgctcat ctccatcgtt ttcaaatgga tataagatta catgatcaaa
    19861 ttccagtcct tttgctagac tcaaaggaat gatttggcag ttttctaatc catatgaatt
    19921 ttttttaatc gattcttgta gcgtttgggt ttcctcttct gtttgggtga taactgccgc
    19981 tttttctcct tttagagaaa ccaaaatctc agctagtatt ttcaacaatt cttcagagga
    20041 tcgaaaagag cggtattcgg gttcttttcc ttgcggtcgg atcgatacga tgtcgatctt
    20101 ctcttgatcc acagcatagg ttttgaaaag ctctgtgatt gctccgctag aacggtaact
    20161 atttctaaga tcataccgat ggatcggcag atggtagtcg tcgaaacaac gcataatatc
    20221 agcaaaagta gtggaagaat taaagattgc ctgattctcg tcacctacta aagtaaagtg
    20281 agtttttgga aataattcga tcaatagacc taattgtgcc agggtataat cttgtacttc
    20341 atcaatcaat agatagcgaa atgctggtac atttaccttt tcaacaagta aatgacgaat
    20401 agctaatata atgactgctt catcaacagt acgtgtgggt tgccaagcat aagctgagcc
    20461 atatcgtttt tcataaattc tttcaaacaa ctgatgctca tccacccatg ccatttcttc
    20521 gatttgtctg gttatttttc gatatttttt tgtaaaagtt tccttgcata agctacaata
    20581 ctttgttccg aatcatcaga aatcaattta ccgaataatt ccaactgcct atcttctgtc
    20641 aacgagagta cctgattctg cacagcagga ctttttgctt gtttcaaaag atgattcttc
    20701 cattcgctca tcagtgcctt tttcgttgct tgaagcctat cgatcaattt aggatgagca
    20761 ggaactttct catatatttt ttcgatcgtt tttttagaaa ggactgtttt tccattttta
    20821 gtaagtccac ggataaaatt cggatgatct aaaaacaaag gatctgattg ttttagatta
    20881 tcgataaact ttttgctacg caaacgttct gtctgctccg atacattttc tcccgtgata
    20941 cgtttgaaat aagcctcttc accttctatt tcctctgctg aaagctgtga aaccagctgt
    21001 atgatggtta gattcagtgg atttctttca cctaatgatg gtaggacatt cgaaatatag
    21061 tcgataaacc gatgattagg tgatagaatc agcaagtcat cggatgtcat cgtatctcga
    21121 tataaataga gcagatacgc aatacgctgc atgatagcag aggttttacc acttcctgct
    21181 acgccattta ccaagatatt ttgacttttc acatcccgaa tgattgtgtt ttgttccgat
    21241 tggatcgtag cagtgatagc cttcatttca ttggtctcat cttggcctaa gacttctaat
    21301 aatacgtcat cttgaatagc tacagacgta tcaaaaaact tgaccaaacg atctctttcc
    21361 aaaagaaatt gtcgtctttc tttaataacc gtatcgattt tattttgacg aaccgtataa
    21421 gaagatggac caatcatgtt attgtaaaaa agttcggcaa ttggtgaacg ccaatcataa
    21481 acaagatttt catctgcatc atctgtgaaa tttgctgtac cgatataaaa ggattctttt
    21541 ggctcattat ccagaaagtc tacaacgact tttccaaagt agggactttt caataaacgt
    21601 tcgatctttg ctagctgatt ttctgcgttt ttctgtttga tattcatctg atcgatttct
    21661 ctatttttca tttcaatcat cgaaaaagta tccaggttat ctaagtaact gtcaaaattc
    21721 aatcgaagct cgccagccat ttctttcaac gactggtttc cttctatttt tgccgtatcc
    21781 atcgattccc gcagttcgtt ttttttatca attagctttt gatacacgtt cttcagatat
    21841 gcttcttcta actgtttttg tgtgttgacc atacacattc tcctcttttt ttaaagtggt
    21901 ggaaccgatt agtttacctc ttttaagagt cgaatgcaag ctttttaacg agtatcattt
    21961 gttaggatat atcagttctt caggtcggac agtttgatat aaagaaaatg caccatcaag
    22021 gttttttacg tggtagccag actgcttcaa gattctttcc gctagatagc tacgtaatcc
    22081 gctatgacag ctgacgatat attcttgttg gctgtctaat tcatttaatc ggtcccgaag
    22141 ttcattcaaa gggatatgtt tggcttcctt gaatttgccc ttttccaatt cttctgctgt
    22201 tcgcacatct aataaaattt ttccttttgc tagttctgtc ggcaattcat gccattgtac
    22261 ggtttcacta agcccttctg caatgttcat tgctgcatat cctagcatgt ttactggatc
    22321 tttggctgag ccaaatggcg gtgcatacgt caattctaat tccggtaaat cgaagatcgt
    22381 caaatgccct ttgattgctg ttgctaaaat atcgatccgt ttatccacac cttttgcccc
    22441 aacaccttgt gcaccataaa tctcgccagt ctctggatgg aagatcaact tcagtaaaat
    22501 atctgttgct tctggataat agcttgcatg atcttttcct gatacatgaa tgacttcaaa
    22561 agacagttcg ttttctttgg ctgttctttc acttaaaccg gtggatgcgg cagtcattcc
    22621 aaacgttcgt ataattgctg ttccaatacc gccttgattt cttcgagaaa ttccggaaat
    22681 cgtgtccgca acttgtctac cttgacgatt ggctggtgaa gcaagagaaa taagtgcatc
    22741 ttttccagtg atttgctgtt ttacaacgat agcatccccc accgcataaa tatcaggaat
    22801 attcgtttga taacgttcgt ctactaggat acccccgcgt atgcctaatt cgattccagc
    22861 ggtcactgct agctggttat caggacgaac accaatggat aaaataacta aatcagcagg
    22921 gatggtacgc ccatcatcca gtcgcaatcg tttcccgtgg tcttcaaatc cagcgacagc
    22981 atgagaagta atcacttcta ctccttgatg agacaattct ttttctataa aagctgccat
    23041 ttcttcatcc agtgttggaa gaatatgtgg tgcttgttcg atcaccatga cttctaaacc
    23101 tcttcttttt aggttttctg ccatttccaa tccgatgaat cctgcaccaa cgatcacggc
    23161 acgctttggc tgtttttcta atgcatgcat cactttatcg atatctggta catttctaat
    23221 agaaaaaaca ttgtcggctt ctgttatccc tggaatcgat ggaacaactg gttttgcacc
    23281 aggagataaa accaatgcat cgtactgttc aatgtgtttt tgaccctcat gtacgacttc
    23341 taccgttttg ttttcgggaa agatggctgt cacttcatga tttggacgca catctaaaca
    23401 aaaacgagcg gataatgatt ctggcgtttg gacaagaaga ttttcccgtt cactgatttc
    23461 tcccgaaaga taataaggca agccgcaatt tgcaaaagaa acatacggtc ctttttcaaa
    23521 aacaacgatt tctgcatctt ccattaatcg gcgaagccgt gtagcagcag acattccacc
    23581 tgctacacct ccgacaataa cgattttcat cttatttccc tcctactgtt cgatcaaacc
    23641 attgattcat gccaccacgt acattcacta catcataacc agatttcttt aattctttag
    23701 ctgcttgttt actcctcatc cctgattgac aaatcacata gacggtcttt tctttgtctc
    23761 catcatatct gtttatactt tgtaatggaa catttttagc tccttttata tgcccgcccc
    23821 ggtattctgc aggtgttcta acatcaagta acgctggatt ttccaaaagc ttttccttta
    23881 attctgggac tgttatcgat tttattgatt gaaatagcaa attcattttc tgcacctcat
    23941 ttacattttt ataaatacct catggggtat attaaacagg agctttatta atatgtcaac
    24001 cagaatgact cttcattttt ttcactactt tttatatttg cataaaatac cgataagggg
    24061 tattatctaa ttataaaaat agaggagaat gtgttatgga gattgataat aaaaatatcc
    24121 tgaaccgttt gaaacgtaca gagggacaaa ttcgtggtat ccaaaaaatg atcgaagacg
    24181 aaaaagaatg tatggatgtc atcactcaat taagcgctgt tcgttctagt attgatcgcg
    24241 taatgggcat gatcgttgca gataatctga aaaattgctt tgagaaacct gatagtaatc
    24301 cagaagaaca agcggctaaa ctagaacaag cgatcaagat gattattaaa aagtaaagga
    24361 atgatttcaa tgaaagtcgt tttccatatt gatgaattag aaaaatggtc agaaacaggg
    24421 aaaaacgtaa aaaacctgat aaaagcttct ccagaaacga cgattgtcgt atctgtaaat
    24481 ggcatcgcga ttaccggtta tctagactct gccaatgcag aattcttaga tttgcaagga
    24541 gtcgtttttc acgcttgcgc caatgctatg cgtgctaatc atatttcaga aagttcttta
    24601 cctgaacaag taatcgttgt accagcaggt gtccttgatt tagttgaact gcaatcccaa
    24661 ggttatgctt atatcaagcc ttagtcagta gaggttgcga cagaagtgat cagcttcaag
    24721 aaatttcggc ttatttttcg aagaagctac ttctggaaaa ccgtgaatca aaggttgtga
    24781 aaacgctgtc ctgcatcaag t
//
LOCUS       DACEKH010000034        24186 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEKH010000034 DACEKH010000000
VERSION     DACEKH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.034"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(70..615)
                     /locus_tag="IW662_001295"
     CDS             complement(70..615)
                     /locus_tag="IW662_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0866129.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(627..1319)
                     /locus_tag="IW662_001296"
     CDS             complement(627..1319)
                     /locus_tag="IW662_001296"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ0866130.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1338..1622)
                     /locus_tag="IW662_001297"
     CDS             complement(1338..1622)
                     /locus_tag="IW662_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866131.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1635..2198)
                     /locus_tag="IW662_001298"
     CDS             complement(1635..2198)
                     /locus_tag="IW662_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0866132.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2426..3088
                     /locus_tag="IW662_001299"
     CDS             2426..3088
                     /locus_tag="IW662_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ0866133.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3212..4408
                     /locus_tag="IW662_001300"
     CDS             3212..4408
                     /locus_tag="IW662_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ0866134.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4509..5885)
                     /locus_tag="IW662_001301"
     CDS             complement(4509..5885)
                     /locus_tag="IW662_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0866135.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5922..6788)
                     /locus_tag="IW662_001302"
     CDS             complement(5922..6788)
                     /locus_tag="IW662_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ0866136.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6877..7902)
                     /locus_tag="IW662_001303"
     CDS             complement(6877..7902)
                     /locus_tag="IW662_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0866137.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7920..8594)
                     /locus_tag="IW662_001304"
     CDS             complement(7920..8594)
                     /locus_tag="IW662_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0866138.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8778..9671)
                     /locus_tag="IW662_001305"
     CDS             complement(8778..9671)
                     /locus_tag="IW662_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0866139.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9745..11442)
                     /locus_tag="IW662_001306"
     CDS             complement(9745..11442)
                     /locus_tag="IW662_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0866140.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11435..12748)
                     /locus_tag="IW662_001307"
     CDS             complement(11435..12748)
                     /locus_tag="IW662_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0866141.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12748..16266)
                     /locus_tag="IW662_001308"
     CDS             complement(12748..16266)
                     /locus_tag="IW662_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0866142.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16559..17017)
                     /locus_tag="IW662_001309"
     CDS             complement(16559..17017)
                     /locus_tag="IW662_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0866143.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17315..17833)
                     /locus_tag="IW662_001310"
     CDS             complement(17315..17833)
                     /locus_tag="IW662_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0866144.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(17835..18452)
                     /locus_tag="IW662_001311"
     CDS             complement(17835..18452)
                     /locus_tag="IW662_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0866145.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18449..19303)
                     /locus_tag="IW662_001312"
     CDS             complement(18449..19303)
                     /locus_tag="IW662_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0866146.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19300..20211)
                     /locus_tag="IW662_001313"
     CDS             complement(19300..20211)
                     /locus_tag="IW662_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0866147.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20252..21586)
                     /locus_tag="IW662_001314"
     CDS             complement(20252..21586)
                     /locus_tag="IW662_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0866148.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21794..22624
                     /locus_tag="IW662_001315"
     CDS             21794..22624
                     /locus_tag="IW662_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ0866149.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(22868..23117)
                     /locus_tag="IW662_001316"
                     /pseudo
     CDS             complement(22868..23117)
                     /locus_tag="IW662_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23308..23502)
                     /locus_tag="IW662_001317"
     CDS             complement(23308..23502)
                     /locus_tag="IW662_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866150.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT         7143 a         5253 c         4166 g         7624 t
ORIGIN      
        1 aaagtcgttt gttttatgct cttcttttat tattttgtgc tttttatttt atcaaatcac
       61 tttgtttttt tataaaattt ctgcgcctag tgtggactca aaatgtctca atgcccattc
      121 atgtccaacg ggatcgaaac ttgccacggc atctttgaaa atatggagtt tgtatcctag
      181 attatacgca tcgactgctg tatgcaacac acagatatct gtacatacgc cggtcaaata
      241 aatatctgtg atctggcgtt ctcgcaaacg gatgtctaga tctgttccac taaaagcgga
      301 ataatgccgt ttgtctatcc aataaacatt ttcctcttgc tcatagtctt ggtataaact
      361 ttttaaagag ccgtacaatt cccgtccacc cgttcctatc acattatgcg gaggaaataa
      421 gcggttctcc ggatgaaagg aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa
      481 atccttttga tcaataaact tctttgtatg agcagtcagc tttttttcaa ttacttgtcc
      541 tgctgtccca gtagtcagct ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga
      601 gatcaatgcg ttcatcttag tcctccttat acgagatgtt tgacaaattc gatcaccatt
      661 tctgctgatc gtttgcctgc gtcttcgata aattcgtcaa agctttgtgt cgccgagtga
      721 tctgctgtgt cgctcattgc acgtacgatc aaaaatggga tattgaactg acgagctgtt
      781 tgtccaacag cggctccttc catttcgcaa gctaatgctt cagggaaatt cgttaagatt
      841 tctttgattt tatctggcga gtcgacaaat gtatcacctg taacgatcaa tccttctttt
      901 gcgttcaaat tcgttgcttt tgctgcttta accatttcac tgcgcaaata tgtgcttgct
      961 tcataatata gaggcattcc tggtaattgt cctgggttgt agccaaatcc tgttgcatct
     1021 gcatcaaaat aagctacttt gtctgatata acgatgtcac ctacttgtaa gccttcaccg
     1081 attccgccag ctgaacctgt attgatcacc atgttcacgc cgtattgttg gatcaacaga
     1141 cttgtggtaa tagatgctaa gacttttcct ataccggaac gtacgacgat cacttcgtgg
     1201 ttacctaatg aacctgaaat aaataaagcg cctgcgcgtt cccatgataa tggttcgctt
     1261 aggttctctc tcaaaatttt tacttcttct tccattgcgc cgataatacc gattttcatc
     1321 tttttaattc cttctttcta tataaatgcg attgctaaaa aaaccacaca taacattagg
     1381 ataacaataa taagagattt catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg
     1441 ctgatccttg ttttcgtgat tggtttattc tttttgcttt ctttacgata aaagctggca
     1501 atcttttttt cttcttgctg ataagctgtt tctgcttttc gctgtttttt taatgactcg
     1561 cttgcttgtg cttgttgtcg cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc
     1621 ataagccgat ccctctactt tcctttggtc aatagttccc agtattgaat cgcataaatc
     1681 gtctttgcat cacaaatcaa ctgatctttc atcgcttgtt gcgcctcttc aagagttaga
     1741 tgatacaact ccagtacttc gtcttcatct tgtgctaaag gattctcgac cttctctacc
     1801 ccttgtgcat gataaatatg aaggacttca tttgcaaaac ctggtgaaag atacatcgaa
     1861 gtaagatggc tcagcgactt cgcacgatag ccagtttctt cttctaattc tctagctgct
     1921 gtcatttcgg gattttgtcc ttctcctgga tcgatttttc cagctggaat ctccaaaatc
     1981 actttttcta aaggttttcg aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa
     2041 gcaatgatcc cgacaccgcc aggatgaaat accagttctc tcttcgaagt tcctccatct
     2101 ggcaaacgaa cttcgtctac agcaacatca ataatttttc cgctatagat ttctttgcga
     2161 ctgattgttt tttcttcaaa ttgctcattg ttcagcatat cttctcctgc tttctttgta
     2221 atcataactg cttttttttc atttttcaat ttttctcaaa aactccttat tagtttacca
     2281 tagataaacc ttagattttc tctaaatata gaaaatcctt ctttagtccg atactcttta
     2341 tagtagtccg tatgctaaaa taagtatgta atcgaataac gaaaagaatt caatactcaa
     2401 cgataaatga catggagggt ttattatgat aaaacgaaat tggccgtggc ttctagcatt
     2461 ggtcttgcta tttctctttg tccgaaacaa tagtttttct tttgttctag gacttttatt
     2521 gataggtgta gttttagttt tgtggggact ctttggttcg cgcaggaaaa agggaaaaca
     2581 agaagaaatt cctgcactga ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac
     2641 agcgaatgaa atttcatttt ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca
     2701 attgcaaatg aatatgcagc agacagccaa acttaaagca atcgatctcc gccatgatcc
     2761 agtcaaagca gcgaaagctt tattcaaaga attggtgaaa gaaccaaacc gactgcatga
     2821 agcaagtcag tttctttata cgcatctgcc gaatctagtt gatttgacaa ataagtataa
     2881 tgagatcaat gaccatgaga tcaaaaacaa acagacctat gaaaaactag aagaaagcgc
     2941 gcagatcatc gaccagctgt cagctttaat cgcacaagat tatcaaaaat tcgttgctga
     3001 ggatctagat gatatcgacg tagagatatc tgttgcgaaa cagagcttaa agcgtgataa
     3061 taaatatgaa ccagaacaaa aggattaact cttttgttca taaaagaaga ctcggaacat
     3121 tcagatgatg tctggctgtt tcgagtactt tttctaaagt agagacattc atgtaagaat
     3181 cgagaacaga ttcaacaagg aggaattttt tatggatcat aaaccgactg aaacaaaaga
     3241 tgttacacca gttaacgata cacttgaaga tttattgaac aacccgtttt caactccaac
     3301 agatacgctg accacttcac aacaagcgga gattgatcag ctccaagagc aacagacagc
     3361 tgctcgttta attgacaaac taccggcgga acgccaagaa caagctaaac agctagcagc
     3421 aaaaatcgat gctaatgatg cacagtctgt catcagctac ggatcggctg cccaagcaaa
     3481 attaagtgaa ttctcacaat cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt
     3541 aggagattcc ttaactgaac tgatgtatcg tctccaagaa gccaatcctg acgaactgcg
     3601 tgccggagaa gggaacatct tccagcgtgt cttcgggaag gtcaaacaat cgatctatga
     3661 agtgacagca aaatatcaaa agattggtgc acaaattgac aagatttcag tgaaattaga
     3721 taaagaaaaa gacggtctgc taaaagataa tctgatgtta gaacagcttt accagaaaaa
     3781 caaagactac tttgatgcac tgaatattta tatcgctgct ggagaattga aaatggaaga
     3841 acttcaaaca acgatcattc cagaagcgat gaaaagagca gaagaaactg gcgatcagat
     3901 ggatgttcag attgccaatg attacacgca attcctagat cgtctggata aacgtacgca
     3961 tgatcttcgc ttagctagac agatcacgat ccagcaagca ccacaaatcc gtttgatcca
     4021 aaacacaaat caggccttgg ctgaaaagat ccaagcatct atcgctacag ccattccgct
     4081 gtggaaaaac caagtcgtga tcgcattgac cttgcttcgt caaaaagatg cagtaactgc
     4141 tcaacgccaa gtctctgaaa caacaaacga cttattgaag aaaaattcag aaatgctgaa
     4201 aatctcagcc atcgaaacag cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct
     4261 tcaaaccact caaaatgatt tgatcgaaac aatccaagag acattgcgta tccaaaaaga
     4321 aggaaaagaa aaacgtcgtc atgcagaagt agagcttgga cacatggaag aagatctgaa
     4381 acaaaaactg ttggatttaa cccaatagat cacctttcgc ttgttactaa aacaaacgcc
     4441 caacaacagt ttattacaac tgttgatggg cgtttttctt atctttcttc taacttctcc
     4501 attggagtct atttctcatt catcttgctg tgtccatagc catagccaaa ataaatgatc
     4561 agtccaataa ccattacgat accgaacacg atccatgtca cagtttgcag acgaagcatc
     4621 aatacaatac aagtcaagat ggagacgatt ggcaatagcg gaacgaatgg cactttgaat
     4681 tctccagctt ttggtttgcc aaacattttt ctcaacttca gtaagcctag actaacaagc
     4741 atcaaatagg cgagcgccat gatattcgtt agttccgcta atacattcaa tgggaaaaat
     4801 ccggcaaaga atacagctgc cacaccagcg gcataagtcg cttttttcgg tgtacgatgc
     4861 ttttgatcga tttctgtcat aaacttagga agtaagccat ccttgctgac agcaaacagt
     4921 aaacgagcaa gtgaatacat catcgagatc gttaccgtca gtagcgttaa aattgcccct
     4981 acagcgatca ccgcgcccac tgttccatgt cctacatacc tcattgcaaa agccactggg
     5041 tcattgactc ctagttcagt atacggaacg attcctgtca agatcagcgt cacgatgatg
     5101 taaaggacag tagcaatcag tatcgaacca atgattcctc ttggcacatc tttttgcggg
     5161 tttttgactt cctcggctgc cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca
     5221 agagctgcac catctagcac accggaaaaa ccaaaaggca tgaatggctg ccagttatca
     5281 ggtttaacga aaaatattcc tacaatgata aacaacgcga tgataccaaa tttgacatat
     5341 accatggcat tattcaaacg taatgctttc ttggcttcaa gcgacacgat gtaagtgacg
     5401 aatatcacta cgagcaccgc aatcaagtca atgtatgtac cattttccgg attgtacccg
     5461 ccagttaatg cttgtggaag ttcggcccct aaacttttca aaaaaccttg catatagcca
     5521 gaccatccgg aagcaacaga ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct
     5581 gtcagccagg cagcaaattc accaaataca acatacagat aggcatatgg cccaccaatc
     5641 actggcactc gcgaagcaaa ttctgcgaaa cttagtccag acaaaatgac aacgattgca
     5701 gctaagacaa atgaaagtga cagtgctgga ccagcatttt gattggccgc aacccctgtc
     5761 accacaaata ttcccgtacc gatgatcgca ccaattccaa gcatgatcaa atcagacgtt
     5821 tttagttctt ttttcatctc actcgtatga ttgagattga tctctttctt acgaaacaaa
     5881 tccattgaag ttcctcccca ttttaggttg gaaaacagcg attaaactgt tttcatatac
     5941 cctaaaagga taccacctaa aatgactaaa aggcaaccaa caataacata aatgagctct
     6001 tttttcgttt ttctttcgcc aaggatgaaa atcccaccta aagtagagat aatgatcccc
     6061 atttgtgaaa gtgagaagcc aacagctaaa ccaagtgatt tgactgttag aagcatgcag
     6121 atgtttccta agccccataa taatccacag atcatgtttt tccacacaaa ctgatcgact
     6181 ttaactttac ggaaagcaaa gaagcttgcg cctaagatca ttccgatact ttgaggtaag
     6241 ataattgaca tcgcatccaa attcgcccag ttgacgatga tggtatatac accataacca
     6301 atcgtagaga agatcaatgc tctaaaaccc ttaccaaaat caagcatttt attatctgta
     6361 tctatttttc cttcgtcatc ttgtcgggca gtcaaatacg caccgcttac aagaagagct
     6421 aaagcaataa taccgtatac ataatctttc gtttgtgtcc attcatggaa gaaaacggct
     6481 cccgcaatcg tattcaaaat taattgaaaa ccagtagaca atggtagccc tacagaaaca
     6541 cccatgtact tcattccatg gaactgtcca ttttgtccaa cactccaagc taagccggat
     6601 aaaaatccga tcaagaaaat ccaaggggtg attacaggac taacaacgaa aaagacaact
     6661 aaagaaaaca gaaatgcacc gatcgtcatt cctagtgttt gttgattggc atcgccacca
     6721 attttaccac ttactaaacc aatactaccc catgccaaca tagggactaa tgctattaaa
     6781 atttccataa ccaatctcct tttatttcac atgataagaa acttatcagc gaaaacgtta
     6841 acaacacaag cacttttttt cgtttctaac gattttctaa cgtttttcat tataaaaatt
     6901 taagattggg aaaaataaag tcattgttgt cccttttcct tcagttgatt cagccgtcaa
     6961 atggatacct aatttttcgg ccagattttt tgctaaataa agtcctaatc ctgttgaatg
     7021 tgttttgcta agtcgaccat ttctcccagt aaatccctta tcaaagatcc gtttcaagtc
     7081 ttccgctggg ataccgatcc ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt
     7141 ttctatgctc acaatgatgt ttccgtgatc cggcgtatat ttgatggcat tactaagcaa
     7201 ttggttgaag ataaaagcga tccatttagc atcggtcaaa acagtatgat cctcaccaac
     7261 gatctcgtag cgaatatttt tttggataaa ataatttgcc tgactgcgta tcacaggctg
     7321 aatgattcct tttagtccat attcttggat gaggtagtcc cgcgagaaac tgtcgagtct
     7381 tgcataatac aaaacttgtt ctacgtattc atcgattttt gataattcat tttctagtaa
     7441 tgtatacttt tgatcgtcaa tatcgaactc gatcgaacga actagcaatt tagaagcgga
     7501 aagtggcact ttgatttcat ggacccatga gtcaatataa tctttttggt cttgttgatt
     7561 actgattgct gattgcatta cttcttggtg ttcacggatc agctgattga tatattcttc
     7621 taccagtttt tcttcttctg tcctcccacc actcaaataa ttttgcaaag gggaagtttt
     7681 ttgtatgtcg gctaattttc cccaccatct tctcttgctc aagtaaccac ccagcagata
     7741 aaaaatcaga aagacgccct caatcaaagc taaataagca attgtgctca aatcaacttt
     7801 gatgtttggc gccagccaca tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa
     7861 gatcaatgtc cactgatctt tcaaatattt aaaaaaattc atcttaaccg ctcatccttt
     7921 cagggaacga tatatccctg tcctactttt gtttcgatat atccttggat acctgcctgt
     7981 tcgatttttc ggcgtaaccg attgatgttg actgtcaatg tattgtcatc tacaaatcgt
     8041 tcgtcatccc acagtgctcg cagcaatttt tcccgactta agatctttcc attgtgcttc
     8101 atcaaaataa ataatagacg atattcattt ttgctcaaat caatgatctc tccgttgatt
     8161 tccatacttc cgttgtcaac attcaacgta atgccattgt ggctcatcat ttcactagat
     8221 aattctgtgt agttgtaaga acggcgaagt aatgcattga tttttgcaat cagtacatcg
     8281 atttggaaat gttttgtcac aaaatcatct gcccccatgt tcatagccat gatcatatcc
     8341 atattcgtat tcctgctgga gataaaaatg atcggtactt tcgatacttc acgtattttt
     8401 tgacaccaat aatagccatc caaaacggga agattaatat ctagtaatac aagctgtggt
     8461 tcttctcttt caaagtctga gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat
     8521 tgccatttct caagttcctc catgatcaat tgtcgaatga tctcttcgtc ttcgactacc
     8581 atgatttttg ccataaaaaa tccctccatc tttcttcttg ctactacaat atcaaagaag
     8641 cccttgtgaa acaagttggc ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac
     8701 tggaaatatg cgatttaatc gcgtttgtct cttccaagca gcttaaattt attatgttat
     8761 tcttaataat tggtctatta tctgttattt ttctttcgtt gtttttgtct tttaaatttt
     8821 cgataacacc aataaccgaa aagcgttagc actagcaata tacctagaag gagtataagt
     8881 aatcgataaa gatggtattt ttttgtctgt tctatctttg aagagacttc ttctgtaaag
     8941 ggaactcgtc ttcctgtcac tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg
     9001 ataagtgtga ctagatcctg cttttctcta attgctaatg tatttgtttt tgttggaagg
     9061 acaacaattt tctcaacgac ttcataagcg agcatccgac catacacttc aagatagaat
     9121 cggtcatctt gctccatttc ttcaagatcc gtaaagagtt ttttctcagc aataccgcta
     9181 tgggcggtaa tcacggaatg tgtactctct cctccagttg gaaaagaggt gccttgcagg
     9241 actgtcgctc ctctatctaa caaaagatca ttcgtttcat caaataaagg caagctaaca
     9301 ttgattttcg gaatatagat ggctccgatc atatgttgtt cgtaatattc ttttcctgga
     9361 ttttggacat ttctaaccgc ttgctcaaaa ggatctttga cttgtcccat tcctgggatt
     9421 actgatgtat gagcaagcgt ctgatttttc ttttcatgca ctgctaattg ttgctcatgt
     9481 ttttcttctt tatcgcgttc aagctgcttt tgataattct cgatccttct ttgatccaaa
     9541 taattgctga gagcatctgc taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac
     9601 atcactgcca taaataattg taataaaaga ctgatttggg tttgaggtct attacctatt
     9661 ttctttttca ttttcttcac cttcttattt aaaaaaactg cagcaacctt tataaaaccg
     9721 attgctgcag cttatttttg ttgcttatac ttcagacttc attttagatt tacggtacca
     9781 gctgtacgct ccaatcatca aacttattcc gattagtaag aatgccaaga gcccgttccc
     9841 accagtagca ggaagtgacc cgcgtttcat attcggtact ctttctggcc ctggattttc
     9901 tactcctacg atctgaattt cttcattata gcttccataa gagatgataa attcagtaaa
     9961 ggctgcctca cctgttggaa gtacataacc ttctggcgcc atggtctctt tcatttgata
    10021 ggttccataa tctaaccctt gaacctcaaa gtatccattt ccatctgcga caaattctgt
    10081 tgcttgtccg cttgttgccc actcaattct atctgctcct gattcataaa cagctaaagg
    10141 cgactcccct ttataaaata ttgcgtattc agaacggtct tcattccaca attcgaatct
    10201 tgcatcttta agcaattcac tgcttgatgc atcatatttt tcaaactttc ttccacctgt
    10261 tgtgactttc tctggcggct ctactccttc ttcataatca aattcatcat ttcgtccgat
    10321 ttcaataacc gctgtgttgt tatggaaatc atccgcttct agttctgtgg aaaccttcaa
    10381 attatacgtg atcgttaaat ctcctccacg tgcatctgct aatctaccta aagtatctac
    10441 tttggctact ccattttctt ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc
    10501 tactatataa tcttgtcctt ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg
    10561 atcataataa gttagaccaa ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc
    10621 gcttccgttt tcaaggacag aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga
    10681 agccgttttt cctctttcta gatcaatata agaaatcgtt tcttgttctc cagcttcatt
    10741 aataatcgtt acttgtctaa ggtcgctttc ctctacattc atctgtttat ctgcttctct
    10801 gatttcgttt ttcggataca aatgcacatc tgtattgtat gtgtctttcc atgtagaacc
    10861 atcccacacg ccatccacca tatcaggcat catcactggc atcataacaa ccattggaga
    10921 cgcgattctg gaaatatgag ctggcgaata agtttctaag aaaagataga ctttatctct
    10981 agaagaaggt gcttcttccg tcacttggac ggtagagaat accgcttctc cagcttcatt
    11041 cgttgtttgt ttgtcaatac ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg
    11101 tttttgaatc cattcaatcg cttcggcaga attaagaccg tcttctcgtt ccggtgttaa
    11161 aggattatct cgaatcaaat cgtaatatac ttccgttacg tcaaacaaat caaaatcgac
    11221 tcctggaata ttttcttcac caaaatccgt agaaaccaat ccgctattcg gtcgttcttc
    11281 cggaacagat gagaaacctt ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc
    11341 gacttcttcg gctttgacta gttttccgtt cccgaaaatc cctattcccg cagtaacgag
    11401 taacagtaca ctcattcccg tgacaatcat ttttttattc atttttttct ccccgttctt
    11461 tttttattaa taaaacagca cctaacaata aaagaattcc tagtaaagaa atcaacgttc
    11521 ttgcttcatt cgtttgcggg aatacggacc attttcctga ttgacctgga ttgcttggac
    11581 cactcggctc tttcggtttg tccgttgttt cttttacggt ctcgttataa acacgtggtg
    11641 ttgcggattc ataggcagag actggaactt tcccggattc taattcagcc atctcctgtc
    11701 cgttaactac tacatattgt ggttggcctt ttgcatccac ccaaaattca gggatgatga
    11761 cctcgattct cttacttgct tcatccatct cgtaaccttc cacacctttt aattcttcaa
    11821 aataataggt gccagaaggc aagaaacgtt gtcctgtcgt aacaaggccc tctttatccg
    11881 aaatgaattt gcttacattt ttgtcattca aaggatcatt gtctgctggt ttgatccatt
    11941 gatttttcaa gtcattagca ggagccatat ctaaataata tttcccccct tctatcatct
    12001 gatatagagc aaaaacagca ccttctaatg gttttccttt ttcagttgtt cctctttgac
    12061 gtccatactt gaagaagtat ggatctctta gataaccgac atttttagga tagatatgaa
    12121 tctcttgcag ttcctcggtt gattctgtcg ggtgataaat tggcaaaata gcagcaattg
    12181 ggatagctgt ctgatcgata tcaacatcca atccaacttc tggatcaatc gattcttcga
    12241 aaatcaaata aactgcatcc cgatcttggt ttttcttagg tgctttgata cgtgcaatcc
    12301 cgtctcctaa tccgccaacc gtatctttcc cagttgttac cgaagtccct atttggacaa
    12361 gcctttcttc tgtgattagc tgacggatat tgtttctgtt cgtttcagcg atttgctgga
    12421 cgaattcttt aggagtcatg ttttcagcag cttttttttc atgataataa tcggtcatgt
    12481 cgtaaatggc aaatgtcgct ccatttagtg gcaccgtctg tgaaatgata tctgctgttt
    12541 cccctgcttc ctcagagata gccaagccat cattttcatg gtattcaaat tgatcgttat
    12601 aatcgatatc tcgaatcatc cgtttatgca acacgaattc cacgacatct tcccctgatt
    12661 cttctgccga catcgcatgt ggaagaagga ctgctgcaag cggtagtaaa acaagtaatt
    12721 gaagcatcaa acacaacagc tttttcatta ccttgctccc tcctttttag attgtctgac
    12781 aaacaaacca taggctgctc ctgcaagcgc catgaagaat gcagcaagca ggatatagaa
    12841 taatcgtccg gatcctcctg tagcaggcaa cggattttca ggatcgttcc cgacactaag
    12901 ctgtaatctg attcgatttg tctccaaaag ttcatattct aactcttgct gtagatcagg
    12961 cgtctctgct tcttcgtttg gataatacga aatctcaact tttagtgtac ccgcttcttt
    13021 tcctggaact acccgatcat tttctatgtc aggttcttct cgataactaa tttctaaaat
    13081 aaagtaacct ggaagttttc tatagctgtc aggtgacttc acttccttga tccgatagac
    13141 cccttccaac aagtctgaag cattgaatag ggaagaatcg tttggatctg gtgccaagcc
    13201 tccctcgatg atttcatact ctcctgcact attttgtttc tcaattgaaa attctgcgcc
    13261 ttcaagacgt cggctattca tactgtcaat tttcaaaagt tccaaatcga ttggcgctag
    13321 ttcatcttct aaggttactt gtagtacacc atcgatgatt tcatagcctt ccggcaaggg
    13381 agcagtttcg tcttcgatga ccgtatcttc aatgataaac ttaccttctt cattcagtgc
    13441 aaactggaac acttgatcag atagccgata cccatttggt gcgacagatt ccgagacctc
    13501 atattcacca atagacagat gcagaggttg gtcgtaattt aaatcgacat acttttctac
    13561 tcctacggca cgagaataga agtatccgat cccaggcaaa cgtttcgatt cttctggttc
    13621 taacggatta ccagtgtatg ctcgatacag cgggaaggaa gaatccccca ctttcctgat
    13681 atcgaaacct gcaccatcta atgtatggcc ttcatggtct aattttctaa ctcggatctc
    13741 ccgataatca aacgtattgt aagcagtgaa tgctacccat aaacgatcct cttcatctgt
    13801 tgtatacgtg agatattgcc aattttcttc acctgtttgc ttgagttgga tagtccctat
    13861 agcattatcg taaagtcgca agatccatgt gtcattttca tcaggttctt tgaaaccttc
    13921 tgggttctgg ctctcaatga ttttgtattc tgcatagttg gtagtttctt cactatcctt
    13981 ataaacaaat gaatctggct ctccgattgg atgaataact ttctcaccat ccacgattac
    14041 atatttcccg tcttcatcta ctgaatagaa cgtgagtctc cctttctctt cctctacttc
    14101 gttcacacca gaaagccctt tcgctactgg atatccgatt tccagtccgt taccatctgt
    14161 ttcataaagt tcaaactctg cgccattgat tggcatatta ctgccttcaa ttagtttatt
    14221 gatattcatg tggattggct tgaattcatt tcgcatttcc agtttgacat acaactcatc
    14281 atactctgtg ccttctatgt taggctcttg tacaacagaa agcttgtgat attctccttc
    14341 ataataggaa ttcggatctt taccttccgg tccgccgact ctcgaaccag atgtatattc
    14401 atctacaagc tcgtagtgac ctaacatctc atcttcgttg gctgcatatt ttttgatttg
    14461 gaattgatac gtctcatcta ctaactgata acgtggtggt ccagcgatct ctctgatcca
    14521 aaatatgtcg ctgactgctc cagtttcatc tcctaaaatc ggcatcttcg tttcaaagat
    14581 cgctattccg tccgtggtag ttttacttgt ttcgattggt tcggcttccc cactctctgg
    14641 attttggtca tataatgcaa attcgactcc agccaacggc tcgccatttt gacccttctt
    14701 gctgacttgg aaattacggc ctttatgttc gtaacgaatg gaagaagatg ggaaatacat
    14761 ccgtccagta gtgatatcat tctcataatc ctccaggttc tgaatatagg ccatattgtt
    14821 cactgtgcaa aaatttccgt cttgagctcc cgcatcaagt tttacttggt acttgatcct
    14881 gaccatttcc ccttcaccta aagaaatcgg agaaaattta taagcttggt tttcccgtcc
    14941 gacatacgtt gtcggagtcg ccactacttc tggtggggct tgtccatgac gttcttcatc
    15001 ccatatttct ggctcttggt acttcatgcc gttttctcct ggatctagat ggtaaaactg
    15061 aatatctgcc gatctgccag ctccaaatgg tgagacatta aaaccagttt catcataaag
    15121 tgtggcttgc tggatcgttc tcttgaaggt agaagagata tcctccaagg ctgtaacaat
    15181 gtcattcttg ttgttcgcat tgtaatatcg cggtgttacg ccatcttctt ctaaatcagc
    15241 aatatttttc aatacatttc gtccaatagc atctagtcgt tgtctcccgc taacgtttcc
    15301 atcgattcct aaaccaatgg agaagatact tggcgggttc tcttgatact tgtttttcag
    15361 ccaatgatga taagcaactg tgttgatcgc agatgacttt tcttgcgaag taggactgcc
    15421 attcgtattg ttattagaag aaccatcacc aacccagtga gtcgctttat tatctccttc
    15481 ttttacctct ccataacgat agtgtacatt tacttcatct tctggacggt taaagtcaga
    15541 tgcataggta actggatgag taaaccctgc attagaagta ttcccagaaa actcctcaaa
    15601 atttctgaac aaaacgtcat tatcttcagc aatcttatct tccatcacag tccagttgct
    15661 ccaagctcca ttagtgctcg tacgataact tgactgaatc ggtgcataac tgaaggtcgg
    15721 tgtcccatct cccacaacga ttaggatttt ttctggattt ctattttctc cgccattgtc
    15781 tttatacaat gtttcatagc catttttaag ccctagtgta agcggagttc ctccgattgg
    15841 tgtacgattt aatgctgtat tctcacttcc cgtcaaacga ttgatatcat ttgtcaatgg
    15901 aaagatgtcc gaactgattt gctcatggtt attgatagct gtactattat ggtaaaaatt
    15961 caccataccg attcgaatat ccagactcgg gtcagagagc agatctttac tcatttcgtt
    16021 tactgcctca atcaacgcag catttttagt ttgactattg gtcccagcgg ttaattcact
    16081 catactcgct gacttatcta gaacaaaaac gatatcgatt ggactgattt cctttttctc
    16141 gcccccgatg acatctagat agacattgta aagggttgtt gcatcttcag gatgtagatc
    16201 agatgctgtg ctgcttggca gaacaggttc tacccatttt ttgatgtacg ccccgttata
    16261 atccaagtaa ctgttttcaa aattccgttc atttccccaa agctgaacgg tttgagctgg
    16321 aacgtttcta tcttcttcgc ctgcagttga tccattatta gtagcatagt cgtaatttcg
    16381 ccagttcgct cccccgtttg tataatcact atcatcgatc aagttctctc cgtcatatgg
    16441 cgagcctttg tctccttgat agcgttcatt gctttgtgtc caatttccta acacttttgt
    16501 atagtcattc gttgtaaact tcatctattc tttcatcaac caccacttga agaggtagtt
    16561 acccttcgtt cacatcgggg atcttttctg tttcaaaaat caatgtgccg gtttctgctt
    16621 cttttgaata aacttttccg acataccatt cttgaccatc gatcgtctcg acttttaggt
    16681 cttctccatc ttcatcatag gaggatagac ctgttccacg aacgtttttt acggtaccaa
    16741 tcccggcggc tgccgcatcg attgctaaac gtattctacc ttcattggca ctttcctttt
    16801 tttcaaaagc cagatgccat tcaagggttt cttctttttc aatatacttc ccttcaattt
    16861 ttgcatagtc ctcatctaag aatacctcat cctcctttgt ttctgttgac tcagcagcta
    16921 catccagggt cgtcatatga tagactgact ggaaatatgg tccaaatagc aaactgacag
    16981 ttactaactg caaaagcttc aattttaacc ctttcacatc cttatactcc tttcaaaaat
    17041 atttgtcttc tttcacaaca atcaacaaaa caatgcccat ctaattataa tttaatttaa
    17101 ttttaatttt aaatcaaatg atatcctttt tagttaaaaa acaccaatta tacaacattt
    17161 attattaaaa ttaaattata aaattttata acgttctcat ttaacaaaac ttaagttatt
    17221 aagacaaatg aataaacaac tacgctttcc tcgaaacaaa aaaataaagc acaaatgcgc
    17281 atttacgcac tcatgcttta ttcgtacaat taattcagat aacggcagcg cataggatgc
    17341 caataacact tcctaaaata gctcctccta aaatgtcagt aggataatgt aaaagtaaaa
    17401 aaatcctcga ataagcgatc aaacaagcaa ataaaaaggc taatattcct gcaactggac
    17461 ttaattcgaa caacgcagct actgataaaa aggctagcgc tgtatgtcct gaaggaaaag
    17521 aagaagtctt cggtttactt gtgtaaagga caagccgacg atttttttca taaggtcttt
    17581 tccgtcttgt cgtttttttg ataaaaagca aaccaacaaa aaaatagatc aaactgccga
    17641 taagaatggc tagaccatat tctctagtat caggtatcaa gactaaaaca gcagcaagga
    17701 gcaaccaaat aaaaccacca ttagagatat tactgataaa tttcaaaaaa ttgatttttc
    17761 gactatccgt tttccgttgc ttggcgaact tgttgataat actatcatcg attttagcaa
    17821 tcaaacgcaa catgttattc ctccttttta agaacctttt cttgcgtacg atggataaaa
    17881 agcaggactt gaaaataaaa caagaaactg aaacctaata aagcaaaata gattggtcct
    17941 gcaaaccaga atgcgacaag aaggatcaac gtgcctgcca aatagaaaag cgcgcttttg
    18001 aaatcattaa aaaacaagaa aaatgcagta atccaagaag cttctttttg ttccagttga
    18061 agcaagattt tgtaactaaa gagaagcgcg cttaatagaa gcaaaaacaa cgtagtatac
    18121 atcaatgtat agccaattcc cccaccccaa tagcggaaaa tctgatagtc gatcaataga
    18181 agaagaaaat aaacacttgc taaactatct tgctttccat aaagagagaa gttctcacga
    18241 aactttttcc agaatagtga accgagcttt ttctcttcat tttttttgta atgataaatc
    18301 atatacagaa ctgttcgact tgcagttcct aatgtgaaaa caggcaagac gcacaggaac
    18361 catagtagct gtaaatggat aagacgtgga atccattcaa tgatttctaa gaaataatca
    18421 ttggcttttt taccaatcga tcgattcatc atccttttga acctcctgtc aaatccatcc
    18481 cttcaatgaa ataacgttgt gcaaaaaaga acagcaaaat cgtcgggatc aagacaagtg
    18541 ttgctcctgc catcagataa ttccattgtg tcgttgcttg gttctgaaaa acttgcaagc
    18601 caatctgtaa cgtgtatttt tcttgatctt ggatatataa cagaggacct aagaaatcat
    18661 tccaagctgc gttgaaggaa ttgatcgcaa tggtaatcaa ggcaggtttc gtcaatggaa
    18721 gcatcaaatg actccaaata tacaaatgat tcgctccgtc gatttcagca gcttcgatca
    18781 attcattatt gatcgataag taaaattgac gcatcagaaa tatattgaac gcattgccga
    18841 agaacgatgg aacgatcagg ggcaaataag tacctaccca gccgatcttg gagaataata
    18901 catattgagg gatcatcgtt acaaaccccg ggatcatcat cgtagaaaga accaaagcaa
    18961 acatcagctt tttcccagga aaatcaagct tcgcaaatcc ataagcaata aaagaatttg
    19021 aaaggacatt accgatcact actagcacag tgatgaataa tgtatttctt gcatagcgcc
    19081 aaaaaggaaa ggcggcaatc gtatccagat agttttccca tacgattttt tctgggaaaa
    19141 aggtcggagg ataacgaacg atttcttcca ttggtttcaa tgaagtaaac accatccaaa
    19201 gcaatagaat gatcaaaatg attcctatta ctgtcatgag gacataactt agtatttttt
    19261 ttgtttgccg attcacactg gtctttttga aataatccat catttgccgt cacctccaga
    19321 ataatagacc cacttaggtg caagcttcaa attgacaaac gtcagaacca acacgatcag
    19381 aaacaggatc attgacatag ccatcgcata acccatatca aaagctacga acgccttgtt
    19441 ccacatatgc agattataaa aaagcaaaga atttgcaggt ccgcctgttc catttttcgt
    19501 catgacataa gcttcttgaa aaatttggaa agaaccgatc gtagaggtga tcacatcgta
    19561 aaaaataata ggagtgatta atggcaaagt aatacttttg aactttcccc aaacgcctgc
    19621 tccatcgatt tctgctgatt catataaaga tctaggaaca ttttgtaatg tagctaagta
    19681 aagtagcatt gctcctccag aactccatat tttcatcaaa atcaacgcag gtttcgtcca
    19741 ttgtggatca aacagccaag ctggtccagg gatatggaac cagcccagaa atgtgttgat
    19801 caatcctgca gatggagaaa gcagctgcat ccataagatg tagactgcta ctcctgataa
    19861 gactgccggt aaatagaaag ctgtacggaa tacactgatt cctttgattt tttgattgag
    19921 tactgtggct aaaaacacac tgaaaatcgt cgtcagcggt acattgaaga tgacgaaata
    19981 aacggtgttg tataacgatt tccaaaataa atcatcgtga acgaacattc ttttgaaatt
    20041 tgctagacca atgaaattca tcttggatgt caaattataa tcggtgaaac taccaataac
    20101 tgaaaaaagc ataggcccta gcataaatac caagaacccg ataataaaag gcgagataaa
    20161 taaataggcc catcttgctt cttttttctt tcggatagat aaacgcttca aattttcttg
    20221 cctcctctgt acctagacag tcaaaaatgg attattgatt ttgcttgact aagttttcta
    20281 ctgctttttg tgcatctgcc aatccttctt ttggtgattt tgttcctaaa agaatctggt
    20341 caatctgttc attcactaat gtcgtgtaat ccggagcatc taaaggaact ggtgtgatca
    20401 ccgtattttt aaagttttcg tccgccattt gatagatcat ttttccttta tccgttaggt
    20461 tgtcattatt cactaaattc tcattggctg tcttgctcgc catgatatca aaactttttt
    20521 cgccgaattt ttcttgtact tctggagaag aaagatattt gatgaattca taagaggcct
    20581 caggatcttt tgctccgtat ggtacttcca agacgaatcc gccaccccac gaccaatggc
    20641 ctgaaccttc ttctttttct gggagctgca tcacgccaaa atcaaaatca tccggtgcat
    20701 tttccatcaa actagagtaa taattgatat tttgggcacg catggctact aatccggaaa
    20761 taaatggatc agatacacca gacccaaatt cggcttctaa tttattgatc gtatttttcc
    20821 cataataatc ttgccagtct tggatccact ccaaggcttc cactttattt ttagtatcaa
    20881 tcttgacttt ttcatctttg tcaaaccaac tagtcccgtt atctgcgttt agcgcccata
    20941 catcagcccc taaattccat aatggataaa aaccgatgcg tgtaaattct tttccttctt
    21001 ttttatctag tttacgtgct gtttcttcta attcatccca agttgtcggt agatcttgtt
    21061 cactgattcc agcttcttta aataactttt tattataaaa aatgacttgt gtatctgtat
    21121 tgaatgggat cgcataagga tcaccttcat attctactgt atcccaaagc tgcggataaa
    21181 aatccttaga agtgtctttt tctacatatt tggagagatt agttgcttgc tgtgcttctg
    21241 ctctttgggc aacggaattt atgtcctgta caataacatc gggtgcattt ccagctgtta
    21301 ttgcagataa agatttagtc cagatgtcac cccatggctg atacttataa tttacatgga
    21361 tctcgttttg tgattgattg aaatcctcaa tgatttcttc gatagcttct tttcgtgcac
    21421 cggatcccca aaaggaccag aaatcaatat tttttcttcc atcacttgtt ttttcagaag
    21481 aatttgctgc acttgaactg gcatcgcttg cattacttga tccgcatcct cctaataaaa
    21541 acaatgctga acttgccagc aaccctgtaa acaataattt tttcatattt tacctccaaa
    21601 ttttaatgta accgatgccg gttttaatat aacgatatca ttttgttctg aaaataacaa
    21661 tttataaggt atgattctaa aaaaattatt aatgatacca atactttata taattcgcga
    21721 gcgctttctt tgaatttttt aatctttcgt agcagaatga gaatgtatta aaaatagatt
    21781 aggaggaatt ttgatgttta gacaccagaa agaattgcag tttgaagcaa aaccagatcg
    21841 tccgaatcca ttaatcgcga aatatctgca ggagctgatc ggcggccaat atggcgagat
    21901 gagtgtagct atgcagtacc tgttccaagg ctggagctgc cgaggagaag aaaaatataa
    21961 agatatgctg atggacattg caacagaaga aatcggccac gtggaaatgt tggcgacaat
    22021 ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga
    22081 tccaacaatc ggtgccatta tctctggaat gaatccacag cacgcgatcg tagctggctt
    22141 aggacctcgt cctgctgaca gcgtgggcaa tccatggagc ggtgcctata tcattgcgag
    22201 tggcaacttg ttagcagact ttagagctaa tttaaatgcc gaatcacaag gaagacttca
    22261 agtagcacgt atttacgaaa tgatcgatga tcgtggcgta aaagatatgc tgtctgttct
    22321 ccttgcaaga gatagctatc atcaaaatct atgggaatgt gcagtgaaag aattagaaga
    22381 aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt gacaaagaac gaaaagacat
    22441 tgcttatgac ttctataact tctcagaggg agaccaaagt agtgaaggtt cttgggctag
    22501 tggcaaagca ctggatggcg aaggcgaata tcaatggttg aaagaaccta agatcgaagg
    22561 cgctgcacca aaattagaac cagtaacacc aaaaatgtat gggacacctc cattcaagaa
    22621 ataaatatct acccgtagaa acacccgttt tttgttccac acgctttgtg agcaaaactg
    22681 actaaagtca ttaatcataa ccacccttca aaagggtggt tttctctaga gctataagct
    22741 caaatcaccg gccagcgcct aaagacgctg gctttcatat tttatgttca agccatattg
    22801 cctttgccag ttttgcccac ctcttaaaag gtttatgtac tactccttag cccttaaaag
    22861 gatcgtctta ctctcgtaca ctcagcttat ctaacgcact atcatgtttt tcttgttctt
    22921 gaatgtactt tttgatggtc gcttcatttt gtcctacttt tgtcacatag tatccttcca
    22981 cccaaaagtg tcgataccca aatttatatt ttagatttgc acaatctctt gatgatctcc
    23041 tgaatattgg ctcgatattg attataaatt atttttcttc tatactttgg tgtgaataca
    23101 atgtggtatt tacacatcta ctttgtacgg gctaaactat ttccctttat tgctccaccc
    23161 gcatagcaag tggttttgta tttcaatcgc tcggcgcgct caattaggtc taaagacaat
    23221 aaatctaaaa acccgctgct tcactgacag aaacaacggg ttttcagaaa aaaatgaaat
    23281 gaaaaagatc tcttacggca atctatttta tttttttgat tttttataaa tggaaaatgc
    23341 ttcaagaagt ccaagaaata aagccgtata aaaacaagaa cctatgaaat ctttatttat
    23401 tagatattca acagaaatcc cgataacact agccaacaat atatttatca gaagtgtaaa
    23461 ataaggatgc tttttataga atagaactag tgttgttttc atagactctc ctttcttcat
    23521 atttccaatt aatcacaaat tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg
    23581 aaaaatactc ttcgactttc cctacaaacc aggccatctt gtgtttaata actataccaa
    23641 atctaaggta cctctaaatc taaattccat ttgtggcggt gacctccaat ttttaactct
    23701 tctatctccc atccagtata cataaccttg atacttcgac cacttatata tcgaacacac
    23761 aaatacctat ccattgcagg agtactacct aacttagttg ctacttgcat ctaaataaca
    23821 gctgaaaaga taggacttac tcttagtaaa gtatctgaac tattaatttt taaactatca
    23881 tttttatcct caactacatg cacatctgtt gcaacttcac tttctgtctc atccagataa
    23941 atgattaagg aatcgggaat tgcaatatct tcagctgacg ctggaatagt tgagctaaat
    24001 tagtcatcat atctttatta taaaataacc aacagcaaaa aatacaatga aaaacaaact
    24061 tggaactaat gtatcattaa taaatgttga aaaattcagc aactcattac tgtatgattt
    24121 tagcagccca accaacatat accctataaa agctaataag atggttagga atcctttaga
    24181 attttt
//
LOCUS       DACEKH010000035        23025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEKH010000035 DACEKH010000000
VERSION     DACEKH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.036"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1049
                     /locus_tag="IW662_001318"
     CDS             <1..1049
                     /locus_tag="IW662_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ0866151.1"
                     /translation="EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQ
                     TLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVN
                     AFKSRALNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTEST
                     YVWNELLQDINSRGVQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVR
                     VKDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFP
                     PSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKG
                     FQQVTDTLVSMFTE"
     gene            1386..1625
                     /locus_tag="IW662_001319"
     CDS             1386..1625
                     /locus_tag="IW662_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866152.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1987..2247)
                     /locus_tag="IW662_001320"
     CDS             complement(1987..2247)
                     /locus_tag="IW662_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866153.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(2432..2929)
                     /locus_tag="IW662_001321"
     CDS             complement(2432..2929)
                     /locus_tag="IW662_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ0866154.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(3059..3769)
                     /gene="budA"
                     /locus_tag="IW662_001322"
     CDS             complement(3059..3769)
                     /gene="budA"
                     /locus_tag="IW662_001322"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ0866155.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3782..5446)
                     /gene="alsS"
                     /locus_tag="IW662_001323"
     CDS             complement(3782..5446)
                     /gene="alsS"
                     /locus_tag="IW662_001323"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ0866156.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(5651..6313)
                     /locus_tag="IW662_001324"
     CDS             complement(5651..6313)
                     /locus_tag="IW662_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0866157.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(6323..7138)
                     /locus_tag="IW662_001325"
     CDS             complement(6323..7138)
                     /locus_tag="IW662_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0866158.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(7400..7843)
                     /locus_tag="IW662_001326"
     CDS             complement(7400..7843)
                     /locus_tag="IW662_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ0866159.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(7977..8315)
                     /locus_tag="IW662_001327"
     CDS             complement(7977..8315)
                     /locus_tag="IW662_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0866160.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(8303..8680)
                     /locus_tag="IW662_001328"
     CDS             complement(8303..8680)
                     /locus_tag="IW662_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0866161.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            8904..10097
                     /locus_tag="IW662_001329"
     CDS             8904..10097
                     /locus_tag="IW662_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0866162.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(10263..10685)
                     /locus_tag="IW662_001330"
     CDS             complement(10263..10685)
                     /locus_tag="IW662_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0866163.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            11080..11274
                     /locus_tag="IW662_001331"
     CDS             11080..11274
                     /locus_tag="IW662_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866164.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            11271..11765
                     /locus_tag="IW662_001332"
     CDS             11271..11765
                     /locus_tag="IW662_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866165.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(11927..13099)
                     /locus_tag="IW662_001333"
     CDS             complement(11927..13099)
                     /locus_tag="IW662_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0866166.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(13305..14120)
                     /locus_tag="IW662_001334"
     CDS             complement(13305..14120)
                     /locus_tag="IW662_001334"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0866167.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(14270..14716)
                     /locus_tag="IW662_001335"
     CDS             complement(14270..14716)
                     /locus_tag="IW662_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0866168.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(14713..15717)
                     /locus_tag="IW662_001336"
     CDS             complement(14713..15717)
                     /locus_tag="IW662_001336"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0866169.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(15757..16086)
                     /locus_tag="IW662_001337"
     CDS             complement(15757..16086)
                     /locus_tag="IW662_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0866170.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(16212..18539)
                     /locus_tag="IW662_001338"
     CDS             complement(16212..18539)
                     /locus_tag="IW662_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0866171.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(19382..19675)
                     /locus_tag="IW662_001339"
     CDS             complement(19382..19675)
                     /locus_tag="IW662_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ0866172.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(20078..21256)
                     /locus_tag="IW662_001340"
     CDS             complement(20078..21256)
                     /locus_tag="IW662_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0866173.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(21360..21674)
                     /locus_tag="IW662_001341"
     CDS             complement(21360..21674)
                     /locus_tag="IW662_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0866174.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            21781..21996
                     /locus_tag="IW662_001342"
     CDS             21781..21996
                     /locus_tag="IW662_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866175.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(22296..22538)
                     /locus_tag="IW662_001343"
     CDS             complement(22296..22538)
                     /locus_tag="IW662_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0866176.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(22525..22953)
                     /locus_tag="IW662_001344"
     CDS             complement(22525..22953)
                     /locus_tag="IW662_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0866177.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         6858 a         4519 c         3888 g         7760 t
ORIGIN      
        1 acgaaaaata tgatcgcact ggttttaatt caggtaattc gagaaacggt tcttactttc
       61 gatcaatcaa aaccgaatat ggtgaattaa cattggaaat acctagagat cgtaatggtg
      121 agtttaaaca acaaacttta ccagcctaca aaagaacaaa cgatacattg gaaaccacta
      181 ttatccattt attcgaaaaa ggtgttacga tgtctgaaat tgctgatttg atcgaaaaaa
      241 tgtacggtca tcactatact ccacaaacca tgtccaacat gactaaagtt ctgactgaag
      301 aagtaaatgc ctttaaatcc agagccttaa atgataagta tgtcgctatt tttatggacg
      361 ctacttacat tccactaaaa cgtcaaaccg tatccaaaga agcgatttat attgccattg
      421 gtatacgaga agacggcact aaagaagtac tgagttatgc gattgctcca actgaatcaa
      481 catacgtttg gaatgagctg ctacaggata ttaactccag aggagttcaa gaagtcttgc
      541 tttttattac ggacggctta aaaggcatga aagatactat ccatcaaatt tatcctaaag
      601 caaaatatca gcattgttgt atccatgtat ctcgtaacat cgctcataaa gtacgtgtca
      661 aagaccgaaa agaaatctgt gatgacttta aggctgttta tcaagctaac tcaaaagaag
      721 aagcgaatac cttcttatcc ggcatgattg agaaatggaa gaaaaactat cctaaagtga
      781 cgcagtcact catagaaaac caagacttat taacttttta tgattttcca cctagcattc
      841 gtagaaccat ttactcaacc aatctaatcg agtctttcaa taagcaaatt aaaagataca
      901 gccgtagaaa agagcagttt caaaatgaag aatcactaga acgctttcta gtcagcattt
      961 ttgatacata caatcaaaaa tttctaaaca gaagccataa aggttttcaa caggtaaccg
     1021 atacattagt ttcaatgttt actgagtaac taattatttt gcaggaggac aatttattta
     1081 cacaaaatta ttgacgctcc cagctatcct cctagcaact atttaaatac ccaaaaaaat
     1141 tacctctaga taatatcata cctacgtttt cacttataaa ttttttcata aaaaaagcgt
     1201 ttagaggcat aatcgttaat acagatggta accaatgtat ctggtttgaa ctgggatatc
     1261 accctctcca tatatgtatt actaagacta atagatacta taaaagactt gatagttttg
     1321 cattgatttt gcctaatact gagcataggt gtatgctaaa cgatactata aggagtgtgt
     1381 acataatgaa aagaattctg tttatcagtt tatcaaattt attgttactt actgcttgtg
     1441 ggacttctgc atcaaagaac ataccttcag atacgcaaat ttcgaatagt atcgattcaa
     1501 caatttctac aaatgagaaa aattctccgg tagaaacttc cgatataaaa gtttctacct
     1561 ctgcaaaaaa cagggtaaag aaactgtcag cacgtctcct ttatttcaaa caaactctga
     1621 attagcttct tcatcttctt cagcaaataa tgatgatagt ttcgcgttta ctcctaacta
     1681 aatgcaacct ttacaaatgc tttacgataa caagcctgag ctaaatgatc ctgatatcag
     1741 ttttgcattt tttgaaatga ttggatcgga tgatcttttt aaaactacta gtttgtctat
     1801 ccgaaaatag gacagttctg gcagtatagg tttttatcga gtttctccac caggcctcgt
     1861 tacagagaca gattctctag gcgaaccttt tgaataaatc aaaaatcatg aacaatcact
     1921 gccaactaaa taaagtgcct tcaaaagctt ggtcttctcg cttttgaagg cactttattt
     1981 aaatctttat gaaactaaag tatgttcata tttgttgcta tagttatata gatattctgt
     2041 tgtttccaac tcataaacgt gagtatattt tgccactact tttccgcatt tttctaatat
     2101 ttcattgcga tcgacgtcat gatattcgtc tacgcagatc acaacacaat tttctaattt
     2161 actgattact tctccaacca ctttttgttt caagccaata ggttggcaaa tatatttttt
     2221 tcctaaaatg atagtttcgt tattcatatt tcatccatcc ttaatgtttt tccattattt
     2281 aaaatgataa taccattaaa atatgattat gaaaatcaat aacacttgtg attttataaa
     2341 aaaataaaaa aaccgaaaaa cagaaatgcc cactgtcttt ccggctcttt ttattcttga
     2401 tttctcttct tttttgtttc aatagacgat tttagttatt aataaatgaa aaagcattta
     2461 tattaaaatt tgctaataaa agatctacat atttttctag aaatgcttga tcgatttcat
     2521 catacccttt cgttactccg caatcgatat ctaaaacacc gattaaatta ccttctttta
     2581 ccattggcac tacgatttct gattgtgcgg ctgaatcgca ggaaatgtaa tttgcatggg
     2641 ttttgacatc atcaacgatc aatgtttctt gtttttcagc tgattctcca caaactcctt
     2701 ttcccatctt gatacgtgtg caagaaactc gcccttggaa cggtcctaaa atcaattcat
     2761 taccatcgaa taaataatat cctgcaaaga cagtctcagg caaagtctca ttcaacaatg
     2821 cagatgaatt tgctaaattt gctaaccagt tatcttctgc ttctaataat ccttgttgct
     2881 gtaataataa aatttcgtat gcatcttttt tctgtttttc tgtccacatg ataaaaaccg
     2941 ctcctttttg atctatggag aagattatag ctcagttttt ttgtaaacgc taggtttcct
     3001 cctcaaaaaa acgagatacg tcaggttaat caccttgaac gcatctcgct gtctttttct
     3061 attctgcttg ttggatttct gctgctaaat cagcataatc aatcgtttcg tgaacaaaag
     3121 tcggatcatc aactggaaaa tgctgtacaa gggacgagat gcggctaatc tcaacttttc
     3181 cttcagccac ttcaaaatcc attacatgtc cgccaaatgt atgtgtatca tcgataaaat
     3241 gcaagtgaaa tcccgctgca gcaactcctt ggaataattc aggcgtaaag aacccgacta
     3301 atgttccttg gacattgctt tgttggaatt ctggttgaga ctctgaaatt tttgctagtc
     3361 ttctgtaagg tttttcttgc ttcggcatga tacggatgtg catatttttg aataatccag
     3421 ttatcttcac tgccaaaaat aggttttccc cggcttctct tactaatatg tcttttttca
     3481 attgttctcc atctgcagtt tttgagacag aaaaactagt atctggcgta aaatctgtaa
     3541 ttgctgcata gggggtaagt tcacttccat ctaattgggc aactgagcca tctgatcttc
     3601 cttgataagc tacgccatct aaaaagatga cttcaccatc aagcccatgc aatgttccca
     3661 atcccagagt tcctttttct aatatatgag caatcgtttc tgttccatcc atcaagccag
     3721 ccatcaaagc tcctaatgtc ccgtgctggt ataagatttt ttcagtcatg tcgacacttc
     3781 cttaatagaa ttgatctggt aagattgttt ctcccaattt ctcattatca cggtaatcaa
     3841 tcggaatatc aataattacg ggtccttctg tcgcaaatcc ttcttctaaa gctttttcta
     3901 attcctctac acttgtagct cggattcctt tagcgccaaa agcttctgca tatttcacaa
     3961 aatcaactgg accaaagtct acacctgacg cccgctgata tttcattttt tcttggaact
     4021 ctaccatatt gtagtgtcca tcattccaaa tcaaatgaac gatattcaat tttttacgta
     4081 ccgccgtttc taaatcctgg gcagagaata aaaatccccc atcgccggaa actgatacga
     4141 tttgtgtatt tggacgcacc aatgcagcag aaatcgccca tggcaatgca acaccaagcg
     4201 tttgcatacc attactgaat aacaaatgac gaggttcata acttctaaag tggcgtgcca
     4261 tccaaatata atgactacct acatctacgg taacagtcat atcatctgtt acttttgact
     4321 gaagcgtgtt aataacttct aaaggatgca agatacctgc ttctcctttt gattgaacaa
     4381 tgtcccgctc cgttaattct gcttgaagag atgctaaata ttcttttgca tcttctgaca
     4441 cttgttgagg ctctaatgaa cctgtcaata aatcaagagt ggctgagata tcaccgatca
     4501 attctctttc aggctgcata aaaggatcga tttcagctgg cgcttcatcg atcacaatga
     4561 tccgcgcatc tttttccgca ttccagtttc ttgcttcata ttcgattgga tcatagccaa
     4621 tagcaataac tagatcactt cgtttcaaaa gcatatctcc tggttggttt ctaaataaac
     4681 ccacacgacc aaagaaatgc gcttctaatt cacgtgagat cactccagct gcttggaatg
     4741 tctctacaac aggcaatgct gtctttccaa ctaattgcct gatggctaac gtttcttttt
     4801 cacttgatcc tcgcatacct actaatagta ccggtagttt tgcttctcta atgcgttcag
     4861 ctaggtaacg gatatcatct gcagaagcag aacctaattt aggatcgctc aatggcttga
     4921 tgacattccc ttggacaggt gcatccacta catcttgcgg gatactgata aagctcgcgc
     4981 ctttttttga actcttagcc atacgataag cattggcaat cacttctgaa attgtttctg
     5041 gatcttggat ttctgcactg tattttgtga ttggctggaa cagtgctgca ttatccatgc
     5101 tttgatgggt caacttcaat agatcgcttc gttttacttg tccggcaatt gctaaaactg
     5161 ggtcaccttc tgctgtcgct gttaccaaac ctgtagctaa gttactagca ccaggaccac
     5221 ttgttgcgat aactactcct ggctctcctg taatacgtcc gattgcttgt gccataaatg
     5281 cagcattttg ttcgtgacga gtcacgatta attcaggacc ttgatcctct aattcattaa
     5341 atacaccatc gatttttgca cctggtatac cgaatacata atcaacatga tgattaatca
     5401 aactttctac tacagatttc gaaccttgtt tcattttttc gtccattgaa catcgtcctt
     5461 tttatgcttt atttttaatt attagttaga aataatcaca aataattgat acagtttcta
     5521 aacctgtttc attcaaaagt atagcacgtt ttatttcatt tgtttagcca tatgtttcaa
     5581 aaaatgaaaa agaatgacag tttttacata aaaactgtca ttcttaattg aaagtttcaa
     5641 tttataaagt ctacagatcc atagttctca tcaatttacg attgttgaag taaatcaaac
     5701 caaatacgat agaaggacct agtagaatac accattgggg ctttacgaaa aagaagagaa
     5761 acaaacttgt caatagtaaa aaatctagta aactcacata ccattttttc aacgtatagt
     5821 agaacgctat ccgtagaact tgatttggtt tcgacgctgg atttctcacc tgaaaatacc
     5881 agtagttgat gctaaaggaa actgataaac aagcaagtaa aacaaacaac ggaatcaacc
     5941 attttccata agaaaaacgt ataaaaaaga aacaatcaaa aatcattatc acactaacta
     6001 tccaagcaaa tagccaataa aaaaatccct ttagccaaaa ttggcgaaac gtctttatga
     6061 acaaacgtac tggtgaaaaa cttttttctt ttgctaattc ttctgtcata cctatcatca
     6121 cgatgcaacc tggaccaata aaaagtaaag aaaaaataaa ggaaaagaca ttccttccat
     6181 ctatcgacaa aaggaacgca gctgctatat acaagatatt gacacaccaa aaacaaaggc
     6241 tacttgccaa tgattgatac aaccaataaa agaattccat ataaacattt ttgtcaaaac
     6301 gctgctcttt cataatcacc tcctattcct tcattcctga tgaagcgatc ccttctacaa
     6361 aatatttttg catcgctaaa aatataaaag caacaggaag aacagaaaca actgaggcag
     6421 ccatgattaa tgcgtactct gcatcaaata gcccaataaa cattttcaac cctaactgga
     6481 ttgttttatt ttccgtagaa gacaaataaa tgaatggccc catataatcg ttccatgtat
     6541 taacgaatgt gatgatcgtc aaactggcaa tcgccggttt tgtaagaggt aaaatgatct
     6601 tgcgatagat tccccactct gataagccat cgatccgcgc actctcacat aaagaatctg
     6661 ggatactact gtagtattgc ttcaacaaga acacaccaaa agcattgaac gcttgtaata
     6721 gcaccaacga ccacaacgta tatgttaaac ccacttttct catcataatg aactgcggga
     6781 tcatataaga ctgccaaggg acagcaatcg tgccgatata tgctaaaaat aaaatatcgc
     6841 gccctttaaa atttagttta gaaaacccgt aagcagcaaa actagaagta aacaactgaa
     6901 tcaacgtgat cacaacgctt aaaaaagctg tattcttaaa aaatgtcact aaaggaatac
     6961 gagaccaaat aaccagataa ttctctaaat gccaagtttt agggatccat tgaatcggaa
     7021 tcgtgaacac ttcattattt gctttgaatg aagcagaaac catccataaa aaaggaatga
     7081 tcgtcaataa agctaataca atcagcagaa tcatacccaa ccattgaata aagggcatgt
     7141 tttttgaacg aatttttgct ttttttcttt catttcgaac acctccaaag ttataatctt
     7201 catttctttg taatgattac aatccagaac aacggttatt atagctgatt catttccgtt
     7261 cttcttatgt ttgtaagcgc ttatttaaca agttatgtaa atctctttat tttttttgat
     7321 ttaaaaatga aaaaatacaa aagcaagact gtcatacgtc tcgcttttgt attgaaattt
     7381 cttcgattag agatacttat tactgttcaa aagcttcttt gaaacggtga atcaattcat
     7441 tgactacagg tgataaaatc cgattctttt tccagactag catgcagttc gtttcaatca
     7501 ttgggtcaag cggaacgaat gtcactcgct ttgaaagatg acttacagca ccttctatga
     7561 ctaaagcagc acctacctga ttctcaatta gcgcaaatac attgaaaatc aaattgtacg
     7621 ttcctgtgat cagcaactgc tcctttttta tgcctaacca gtcagcaaac attcgctgta
     7681 tttctggacg ctttgagatc aaaagaggta cattcgcaaa gtcttctttt gttatcacgt
     7741 ctttttttgc taaaacagaa ttttctaaaa tgagcaatcc ccatttttcc tttcttggta
     7801 acaaaatcgt ttcgtatttc tctgtatcta ctggatctag caaaatcgct aaatctaaca
     7861 ggcttttttc tagtcactcc gtgatatcat cacttgttcc gctgtggata tgaaattgaa
     7921 cgctgggata atcacggatc aacttttcta gtaatagtgc caatgtattc gaattatcag
     7981 cttccacaca gccgatcgta aatcggccgc tgaataattg tttttttctg ttctcgaatt
     8041 cttgagtggt ttgattcgtt aaatctaaaa tttcttctgc tcggcttttt tagaaaataa
     8101 cctgcttctg tcaagatcat tttgcggttt tcgcgtacaa acagttctgt tcctagttcc
     8161 tcttctaact ccttcagttg tcggcttaag gttggttgtg taagatgtag aacttctgcg
     8221 gcttgagaga ttgttccttc ttctgcaatt gtccaaaaat attttagtaa gcgtaactcc
     8281 atagcgatgc tccttgattt ttttacttga tacattccat tttatttcta gataacacac
     8341 gatcatattt cttgattttt ccttgtaaac gttccaaaca ctcttgcatt tcagcaattt
     8401 ctttcgtcaa aagttcactt tcttcaatca aaagcttacg ccgctcaaat acagttgctt
     8461 ctccttcgct agataggcga atatattcaa tcaaagactc gatcgataat ccggcatttc
     8521 gcatacagat cgtaaagtcg acccatttta ggtcttcttc tgtataatca cggattccgc
     8581 cttttgtccg attaactggt ggaatcaacc cgattcgttc ataatagcgc aatgtatcgg
     8641 ccgaaatgcc cttttcttca ctgacttgtt tgatattcat ctgatcactc tttctcttaa
     8701 tttcttgcca ctttacttat gtattttatt tattctacaa ctttaagtgt actccaagtc
     8761 aatactcatt tctattaaaa ctaagctgga ttatctatat cggttgtttg tgtattattt
     8821 caaataaaaa agtcttgttt ttttattctt ttttggatat acttttaaca atttagggaa
     8881 attttaggaa aaggggtata aaaatgacaa cgtatcagcg aaaggtatta ttatcgacat
     8941 cggcgggaat tgctttagag aatatggata ttatgttttt agctttttca ttatcttcaa
     9001 tgattacttc ttttcatatt agtggtacgc aagcgggttt catttcaacg atcactaatt
     9061 tggggatgct cattggaggt attttttttg gcatcatagc tgataaatat ggacgtgtga
     9121 aagtattttc tcaaacggtc cttctgtttt cgattgcttc tttattgatg tattttgctt
     9181 caaacatcta cttggtttat ctctttcgct ttatcgcagg tgtaggagcg ggaggagaat
     9241 acggggcgtg tatgtccttg atttccgaaa gtttttcaaa aaaacaaatt ggacgagctt
     9301 cttcggttgc tggtattggt gcgcaggtcg gtgcggcttt ggctgcgatt ttagctgctg
     9361 tgatcattcc aacgtttggt tggaagatgt tgtatgtcgt aggtgtactg ccggtgttga
     9421 tggtcttgtt cattcgtcgc ggcttgactg aaccaaagga attcaaatct gtacaatctg
     9481 ccgaaaagaa aaccaaactt agcagactgt ttgaatcaaa acatcttgct tggcaaacat
     9541 gtggccttag tatcatggta actgtccaaa ttgccggcta ttttgggtta atgaattggc
     9601 tgccttcaat tatgcaaaat cgattagggc tttcggtttc tgggtcttca ttgtggatgg
     9661 tcagtactat tttaggcatg tctttgggga tgatgacttt tggcataatc atggataaat
     9721 tggggcctaa actagccttt tctttgtttt taatttgttc atccttatct gtctatctac
     9781 ttgtgttagc ccatagccag tggagtttgg tagcagccgc tgtgatagtc ggctacttta
     9841 tcaatggcat gtatggtgga tatggcgcag tgattagtag tttgtatcca acagaaatcc
     9901 gcgcgacggc gaataatttc attatgaacc ttggacgagc tgttggtggt ttttcttcta
     9961 tcgtcatcgg tttcttgatg gatcattata gtttaactgc cgtaatcttg tttttgagcg
    10021 gtatttacct agtcagtctg ctggtcttga caactcttac tggtgttaca gcattaaggg
    10081 aaagcttgca gcattgatag ttgagtctgt aaatgacaag aaagaggtgt ggcaaaagct
    10141 tgcatctgcg gtaataagac cagccaaatc aaaaacatga gacactgttt ccgtttggct
    10201 ggtcgtatta cttgatgcag ggcagctttt tcaactgcta atttccttct atctggcggc
    10261 gactattctt tgacgtccgt catttcaaag tactttacta ataacttagc ataacgaaaa
    10321 actgtggata cactcgtata ttgagcgtct gcataatctt ccaaattttt ctcaggatgt
    10381 tgatacatga attcaatcaa atgataatta tatgattttc tgtagaaatg aagataaata
    10441 atgtagattg ggaagttctc ctgcttttcc atagaaaagc gccgattatt ctcattcttc
    10501 aaaatgacct tatcaggata tttttggaac aattcttcta aatctgttag ataattcaag
    10561 atagacttac ttgatacctt caattcgttt tgtagttctt ctattgaaca ccaacgttct
    10621 gtttcaagca gtaaatgaat cattttaatt ttcttttgag aaacttttgt taaaaaagtc
    10681 tgcatatttt ctcctcctgt atctacttta ctatactaca tacaagaaaa aaaacggata
    10741 caaatatcat attgtcacac cgtcaatagt gcataaacct gttattattt ctcttttttt
    10801 cctaagtatt ctattgatat gaatcattta atcgtttatt atataaatgt caataatcta
    10861 ataataaaat aacgtacata ttttattatt gcatacttaa taacagaact atagcaaaaa
    10921 atcccgtgta tcaacacggg attagattaa gtttcctcaa tcactattca taacacaatt
    10981 attataacaa gtaagtaaac aattgaaaag gacatttatt gaaaacggtt gttttttact
    11041 taaggaggaa atttagcgaa aaaattagtt gcattgtttt tggtcgcagg aagtttgttg
    11101 agtgggtgtg gtgctactac gtctactact caaagcacaa gttcagatca ggcttccagc
    11161 agcagtacca cgatctccga aagtacggca gattcatcga aagaacaaga acaaattgaa
    11221 aagaaaaaag aagagaaaca aaaagcggaa aaagctgcac tagaaaaaaa gtgacagaag
    11281 cggaatctgc tttgtcacaa gctgaagcaa atcctacaaa agaaaactat gcaactgctt
    11341 cagcagctat ccaagcgcta cctgaaaaca aacaagagtt gacaagccgt cttgctgcaa
    11401 ttgattccac aatcaaagca aaagaagcag aagaagaacg gaaaaagcaa gaagaaattg
    11461 cacgacaaca agcagaacaa gcgcaacaag aagcaatcgc acagcaacaa gcagaacagg
    11521 cgaggcaaga agcaatcgca cagcaacaag cagaacaggc gcgacagcaa tcagcagctg
    11581 aacaggcctc tcagcaggca caagccgcag ctcctgagca aaataatgaa caaactgtct
    11641 atgtcacacc gaccggttca aaataccata cacataaatg tggaaatggt acgtataccc
    11701 ctgccacttt aagcgaagct caagctcgtg gtttaacccc ttgctccaaa tgttatggaa
    11761 actaattcaa aagttatact ataataataa aattcttctg aaacataaaa aaatagaaga
    11821 tggcgttcca gatagtaaac caaaaataac agtttacacg tccatttcgt cagcttctct
    11881 cttttttcat ttagtctgtt ccctctgctt ttttggacat cttcattcat tctttctcaa
    11941 tcaaatagta cacctctttt tcttttcgca gtttatatct tccttttttc aatttcattg
    12001 aaaactgttt cgaatgttta tgttttacat ggattttaag caggagctcc tcatgttgca
    12061 agcgatagtt cctgcctttt aaatcagaaa atctcactag tccgtattca tcagaagctt
    12121 tcacaaatgg aactttttct ctagtaatat agtgtttgcc gttgagcgtt tttacagaaa
    12181 agaccattcc ttcgattggt cgtccgtttg tgttctttac ataaaaagat aaaggatagt
    12241 ttcgcttagc gatggccaaa gaatctaaat aagcgatgag tagccaaact aagccgattg
    12301 atccaagtag tataccgata aaccaaataa aaaatttcca ttttttccat tgatcaattc
    12361 cttggatcgc tttttttgcc tcttttgctt gataaggaat acgatgcccc gtcacaagca
    12421 gtcgatgtgt attgaccata taaggagtac aggtaagcaa agtcacgaga tctctgcctt
    12481 tttggatacc caaagaatct acttcatcag gtaagaccac ttggattttt tctacttgat
    12541 acgccaatgt ctttccattg atctggatga aaaactggtc tccttttttc aacttaggca
    12601 aatctgtaaa taattttgct tctgggagtc cacgatgccc agaaagaacg gcatgggtgt
    12661 ttttcccgcc agtcggataa gaactttctt ccaataatga cgttcctttt tgcaaaaata
    12721 tttccgttgt ctgatcgaat ataggtaagc tgacattgat ttttgggatt tcgatgactc
    12781 caagcatatg ttccataaaa aaggcattac taggtaaatc ttttaatgct tttgcatcaa
    12841 ctgcttggtt gaaactgcct atccctggtg atacgttttg ctctgccaac tgttggtttt
    12901 tctttgtcat tttttcctgc tgttttttta tttcctctga attcttattc gatgcttgct
    12961 tttgataatg gatgatcatt tgctgggcaa gaaaatcatt cagtgaatct tctacaaaag
    13021 gataagaaaa gatcccgatc ccaacgacta aaatgattgc catgaagatg tccagtattc
    13081 ttattctgct tttattcatc atgtccacct attttctaat atgtatatga agaggttgtg
    13141 aaatcagcgc cttgacctga gtattagcga agcaaacggc aaaatagttc ctcctatttt
    13201 gccgtttgtt gagcgaatac caagagaccg accacttgtc acaacctttc tccccatttg
    13261 aatcactatc gtttatttat cgtatgattt ttgtttccaa tagtttaagc ttctgtttct
    13321 tgatggcgtt ttttgaaata gatcactgct cctccaacta aagcagtacc taccaagatg
    13381 aatacgatga tccccatacc accagttgaa ggcaagattc ctttaggttg gttatcgatc
    13441 gttgtttcat cagaagttgt tggatcatca tctactgtaa attcagtgta cgcagcttca
    13501 ttcttaggta atgcgtagcc ttctggtgct tttgtttctt gaaggcagta tgccccttgt
    13561 ttcagccctt cgattgaaaa tttcccttct gaatcagaaa tcaatttcac ttcttcccag
    13621 tcagaacctg tgacttcatc tgaccaagca tatgggtatt ctcctgaggc aaggtttgct
    13681 atttttttca ttaatttaac agttggatct tttgctctta gaatagaaaa ttctgcatcg
    13741 gctaattttt cttgcgtgtt cacatcttgt ttgatgaagt ggaaatcacc gtagttttgg
    13801 acattttcta cagtaatcgt gttatccgta gttgtatttt cccgattgac tacaccgttt
    13861 ggattcccaa gattggcttc atctgcgcca tggatgatac cggttgtttg ttgcaatttt
    13921 ccgtttttta ctataaaagt agtttctttt tgtggattgg ttaattgata accatctggt
    13981 gcttttgttt caactaaagt ataattgccg tctattaatc cattgacact gatggatcct
    14041 tcagtatcag ttgtgaatgt caacgctgac tcagcaggat catagagagc gacaccagtg
    14101 ttttcatcaa aaccggtcac gaattgattc tctgaatttt tgatctggaa ttgcgcccct
    14161 gctaacttat ctacccctga aaagttccca gctttctgga ttttgatatt gctttctttg
    14221 atgacatttt tagcatatag gtgaatcgtg tctaaaggtt tgtcagggtc tagggtaagc
    14281 ttaacacgat atttggcgac gtttcgacac cgggctttgg tgtttctaca aagacgtata
    14341 cagcatcttt cccatcagat ttacctggaa gagtcaggtt agctaatcct tcaccagaag
    14401 tcgttacact tgcgaagggt gtagcgtctg ctggaactgc tgtgtcttta cttggatagt
    14461 acgtagcagt gatgtcatat acgttgaatt caacgccgtt caacggttcg ctattcggaa
    14521 atgtcaattc cgttccgttg tttatttgat tttctggtgc tgaagtgaat ttcaatttat
    14581 gcaaattaac cgtaacattt tctggtgtag gtgatacggc tgcagaaacc tctgcagttc
    14641 ctaggccgag aacgaaagga agcagcatga tagcagttcc taaaaacgtc catatttttg
    14701 ttttgatgtt tttcatttta tttccctcct attgtttcct ttttgttgtt ttttataaaa
    14761 ccaagctgtc cctgttgaaa ggactagcaa taaccctaaa attgagaaac ctccaagcat
    14821 ctcttcgctt gtttttggaa agcttcctcc atttttttca tttgatggac catttgtctg
    14881 catctgaact ggcgtttttc cattattcgt cggatttgct tttggttcgt tgatgatttc
    14941 caatggtata ggatgaagcg gatcataact gcctttttca acttcaaaga gaatggttga
    15001 ttgactcaat atatatcctt taggcggttt aatctccact agttcgtaac ttccaaaaga
    15061 caatccatgg acagaaaatg ttccatcttt actggaagta aaaatcgttg ctcttgaatc
    15121 atctttggct acccaatgat ttatcccgtt ttcttgcgct aagtattggt catttttttt
    15181 gactagaaat tgagcatctg ctagtttttg tttcgtttgc cgatccactt tgacaaattg
    15241 tttgcctcct gtttggatgg caagatgtgt ttctactgtt tgattgtcag tcgtcaaacg
    15301 tacatcgtta tcaaatgtcg ttttttcact gttttctttc aatgtcattt ggtaacggat
    15361 cgttaatttt tttccagcaa acgaactaag atcttttcca gaatctgata agacaaaacc
    15421 atgttttatt ttttgtgtgt gaaatgtatg aatcttgtct ccaccaatca aaaaatcgat
    15481 cgtttctggt tctagccata actgaggatc agctgtatct ttgatcgtat aggttcctat
    15541 ctcgttgatg ttctcaggaa ttgttacggt gatgagataa gggactttat ccccatctgc
    15601 aaaatctgtc tgcttattct ctactgtttt tgttaatgtg agtgcagcag gtattttttg
    15661 ttcgctcttt ggatacaagt ggatggtcgt caatttatga ttagatgaat ctgtcacagg
    15721 aagcacaacg acaaatggtg ctgatttttc ttttacttaa tctgaagtct ttatttccgt
    15781 aaatcggtaa acagcgtcac gaccttgctc atctttgtcc gatgcactaa aagaagcaat
    15841 gccctcttcc tcgtctactg tttttgtcac attttctgcc agtatttttt cactatcact
    15901 tttttgtgct agttttcttt gtgcgtcttc cacactactt ccttctgacc gcagcttgta
    15961 aaattcatcc gagacatcat atatagcgaa agtaacgccg ttcaaccctt ttactccttg
    16021 aaagttatcc gggatattta ttccgtcgtt ttgactgata cttggcatct tatcgctttc
    16081 gaacattcga ttatgcaaag taaatgtaat aggtgattct ctagctgaca cagtcggaga
    16141 acctataaaa aagaccagta ggataaaaaa gagaacagca aatatatcca cacgtacatt
    16201 ttttgttttt ttcatcgctg ttcacctgct tttttcttac gatggataaa ataaaagaag
    16261 ccagaaaaaa taaagatcag tcctaaaaag acaaagggca gtagacctat cccacctgtc
    16321 gaaggaagca agagagtcgg attgatcgtc aacttagcgg tatctgttgc gataatcgta
    16381 taattattgt ttacacttcc ttcgcctaaa ctaatcgtta ctatttgtga tccaatatct
    16441 gtccggctgt taactgttag ggtataatcc aatgattcgc ctaagacaac accggtaacg
    16501 tggagatttc caaccgaaat cgagtgactt tgggcatcat aattaaaggc agcgtcatcg
    16561 acagtaatca ctgcgttagc tttatagatg gtataagttc ctgatgtgtt ggctatatca
    16621 gtaaatgaca acttgggatt tgctgctttg actttgtcta ttccgctatt cgttagttta
    16681 attgtatagc tgccagcatt aaccatattt tttgctgatt gtgcatctgc tgtgtaatcc
    16741 atactgctaa gtgccacttg tactggagta agcgaactag tgacaccctc tgctagcttg
    16801 acatggattg acgggacacc tgcttttcca tcatatgtct tactgccact gagctctacc
    16861 gcaccgctga tttgcttaat cgtatagaca tcagatgtct gcacgatagt aaataaatag
    16921 tttgttctgg cagctagttt agctagtccg ctttttgtca attgccaaga cgtataggtt
    16981 ccaatttcag taggcgcagt tacttcaact cccttttcat tcatcgcttt gaaatcagtt
    17041 ggattccatg tgatttgatc aatactgaga tcattgttta aaagggatgt tgattctgtt
    17101 ttatcattaa aggcatattt aacgatcggt acaacagaaa gaccatcata gtacttactg
    17161 ccatttgcat tgattgatac tgttgcattt gtcttataat ataggatgac ttcagaaatc
    17221 tgttttccat ttgcgtcctt tacgattaca tttccatttg tatctaaact agctaaaaca
    17281 ctcattgctg attggttctt tgtttctatc gctgaaacag ctgtaaaggt atatccgttg
    17341 atagtaggcg tattagtcgt caggtcttgt gcagcacttc cgtaagtaag attcattatt
    17401 ttagaaggag caatcatctg tccaaaatag tcttgatact taatagtgat tggtgtttgt
    17461 ttgcctgtat aggtcaccgt atcgacgtta ctttctaggg ctgtgaaaga accgtttgtt
    17521 tgtgcaaaag aatagcccgt tactgctgtt ggagtgataa ccactttgtc acccacatgg
    17581 attccagata atgtagtccc gtacgttccg atttttgctg ctccaccatt gactgtataa
    17641 gaaaaagttc ctgtccgatt cgcattgacc tgaaccgttg ctgcttgata ggttggaaaa
    17701 tttgaaacat ttcccttacc atcaacgatc tgcccatttt tgatcgagta ccccgtgaat
    17761 ggaatatcat ttgctaactg tataccgatt gaaaaagtac tcatacctcc tccggcagga
    17821 agccctcccc atgttgaggg tgtaaaagaa taacttttat ccgcattggg tgtaactgaa
    17881 tcgatcgtat ttccagcggt agccaccccc atatagatac tttttgggaa tgtgatagta
    17941 gtaccattta ctgtggagcc aataaacaga ttatggatat ctggacggtt atcaataaaa
    18001 gcgattgctt tggttacgcc ttgtaacgca gttttatccg cactcatcga tccgttaacc
    18061 gcaacaatga ctccatcttt ggcatatatc actgtatatc catctggtat ggtatgtccg
    18121 ttgaaattcg cagtggccgc aacagtacta aatgtctggc ttctacctgt ttctgaataa
    18181 gctttctccc ctgctgcttt tgctgctgtg atttcttttt ctgtcggatc aatcagttga
    18241 gtcgaggatg tggttgaggt tgtctcgttg ttgctagact gattggtgct ttctatggac
    18301 tgtatttcag aatagggtgg actatttgtc tcctctgtcg tttggttagc aaaatttgac
    18361 gtttctgtat aaacgtttac ataaatactt gataagctcc ctaagaaagt aaaaatcaag
    18421 atgatcgaaa tcataatggg atgtaagtat tttcttttag atacagcttt gagtctagaa
    18481 acaaaatgtt tttgacatat acgttggatt ttattttgag ttttcttaat cattttcatc
    18541 cctcctctca ataaaaaatc atcaaataat cctttacacg ttattctaaa tttaattttt
    18601 attattttat tgatgtatag aaagtttggg gctttttttc tctttttatt ctaaggttat
    18661 tttagtcttg tcctttaata caaagatttt atttttaaat aaaaaatact ctttttttac
    18721 gattattgaa aaaaaaagat aaaacttgat taattgaagt attgcttaaa tttggaagct
    18781 atgagggaag aataaaagag ttaggacggg attatctact atttttcttt gttttaaata
    18841 tttgttatct ttgaacataa aagagaaatg tgtcgactgc agtgttgtgt ccctcatcac
    18901 gaataaacgg tgttcaacta gctgatcttg tattcactta aaaaatggta aagtacgagt
    18961 tgctattccc cattttcaat tttctaacgc tcaggacaaa atactgtttt cacaacctca
    19021 ttggaataaa agcatatata ttgtttcaag aacgaaatct tgttatcttt ttaaagggca
    19081 tctgtccaaa atctaatttt aggaggtacg ttttggtatt tattcgtaca aataaaagag
    19141 aattatcttt attcatgtaa cgcatccaat tgattttttc atgtacgctc ctggaacgat
    19201 tttactgacg attgcttatt taactgcgaa tcgctcatta gcttttatca tagctatcca
    19261 tattttgaat aacgttcttg gctttgtatt atagatacag atgacaaaaa aagttcaagt
    19321 atgcggtgta gaaccaatat cgcatacttg aacttttttc aatgagtata aattaaaact
    19381 tctaattttc aattcccatc atctgatatg cccgaccgtc tgaacttctt ttgaaaaagc
    19441 caaatgtgat tagataacgt ctttgttcga cataatcgcc actagaaaat acgttcagca
    19501 gtatcgaatt aacctctgtt tctgtataag tcactccatg gtcaaagcgt ttcaagactt
    19561 ctttcaatag gatgtatttc ttttttgttg aacttggtac ttgttttact tttccatcat
    19621 tcatgaatgt gtctaaaact ttcatctttt cttgctccgt ataagtataa accatcttct
    19681 ttctcctttt gattctaaga atactccatt tatgttagaa tattctggat tttttattaa
    19741 agcggttact cttaagaaag atattatcat agtgctcttt tttttactac tacttatagg
    19801 tagatttatt ctaatccatt attctcccct accaataatt aaagaaaaaa ctggatcagg
    19861 gtgttcaaat tacatgctaa gaaaaaagaa cccttgaata ccaagggttc tttctcagtc
    19921 tgataacaat gattaacgac ggatttcttt gatacttgtg aaaagtcgtt aaagtattga
    19981 tataaaagga tttttattac ctctgactac atattgacta atttgttaaa aaatcgtttt
    20041 tcaagaatcc tgtaatacca gtgctttcac cgttttttta actctctaaa gaactaaaag
    20101 caatcagagg ttttaccttt tcatttgacg ttaagtggcg ataaattttc gaagttgttt
    20161 ggtcagtatg gcctaagcga tctttcaaga cttcccagct acagtgttcc gctactaagc
    20221 ttgccattgt atgacgagct aaatgagtat gaaatggctt attaattcct gagcgattca
    20281 cgcaccgttt gacgtgatca tttacatact ctcgacgata aggaacacca cgtttactag
    20341 aatcaagata cacaaacaaa tatgtttctt ctataataaa cctgtaatgt tgcctcatct
    20401 cacccaaagc cttaatcaac tgttgtagct tttccataac tagaggagtt actagaactc
    20461 gtctagcaga actaactgtc ttagtagttt caattcgaaa atccttactc ttacctttaa
    20521 ctgccgtttg cttatagaac agaatttcat ttcttttgaa atctatatct ccaggctgta
    20581 gagcaagcgc ctcgctgact cggcagccag tgtaaaatag aacatggaaa agtacagaca
    20641 tcggtaattc ctcatgaaca atgccataat tcaaaaaagt tctcaattca tcgacagtaa
    20701 gaaacttgtc ctcaatatta tttatggctt cttttaaatc agttaccgtt aactgatatt
    20761 ttggcattct cacagacttc attggatttt cacttatcaa attctttctt tgacaatatt
    20821 taaaaatcat atttagaact tgaataagtg attgtaaact atttttggca tagccaacat
    20881 ttgttccatt aacgccacat tcctttaagg taaaaagcaa ttcttgaagg cactctggtg
    20941 tcaaatcaga caacttttta tcaccaatcc atcgaacaat aatttttaat tgttcctctc
    21001 gcttatcata ggttatttcc ttttgttcat ttaacgaacg gtaatattgg aaccattgct
    21061 cacctacaac actaaacttg tcattgcttg caacaatttt tttcttaagc ctttgttgtt
    21121 tctccatttc cagataagcg agttgcgcct cacgttttga attaaagcca cgtcttgttg
    21181 ttcgatatgc ttgaaagtac catgtgatcc taccagtaga actgatttgc ttcttgaatc
    21241 gatctgtttt ttccatttat tgattcctcc tattttgaag gaacccaaca agcagaaacc
    21301 ctttggaaat ctttctatct atgtcatcat tgtaccgcat cttagaaaga aaatgtaact
    21361 tataaagatc gaagtagtcg caatttttct cgatagtcat tcgaatcact ctttctcatc
    21421 cattctaaaa catcatcacg aaaatagtgt cctcgaatcc cgtaccaaca gggaggaaac
    21481 tcaccaagtt ttactaaacg gttcagtgta ctatcactta tctgaaatag tcgctttaaa
    21541 tcagtatttg ttaataccag aggtaaatga ctgactgcaa gcaatttttc cgtgtaatct
    21601 tccagttttt tttgtaaaat tgaatagaac tctatttcca gagaattaac atttgtttta
    21661 acttccaact ccatcaccct tctctctcaa aatcaaaacg accaccacgg taccccttaa
    21721 aattgtaaaa aaacactcca gtactccatc aaaacggact agaaaagagg aatttttaat
    21781 atgaaacaag gaattgcaga cataaaaata attaaagaaa ttcttgaaaa atctacggct
    21841 aatgcaatag cattcggaac aggaatcaac ttgagtacag taaaaaaact taaaagtgga
    21901 gagagagctg aagaaaaatt aaatcttgcc gatgctatta aaatcacaga atttggcatg
    21961 aaaaatatgc caaccaaaat cgaaatatgg aaatagtagt ttttctgatc tatcagctgt
    22021 tttgccaaag cacataggaa tctcacctcc ccccagtaat ggcgggccga tttggttacg
    22081 gaagtatcat tatcgtagtc gcgattctca tattaaaatt tgtcgcttaa aagctaacct
    22141 tgtcatggcg taactagaga atcaaaatct acatgtactg atagatttgc tattcatttg
    22201 tcaaagagcg ctaggctatt ttcagcctat ggaaatcctt tattttgacc cgataaaaaa
    22261 cttccataga atgaaaagga aagggaaaaa atactttaac gagttgtatc aaattcaagt
    22321 atcttctcaa tcaattttgt ttccagttgt ctacgcatat attcatctac atacataacc
    22381 attccttttt cttcttcata aactggtttc atggctaacc gtgaaatata attttgatat
    22441 tttgacagaa cgatggacat ggcaattgaa tcaccagaag ctgcggcttt gattgtcgtt
    22501 agtggtatga atggtatata tcgtttattt tgcttcatat ttgacaccca aatactttcc
    22561 tagctcttcc aaagccattt gtcgttggta ccaaacactg ccgagacttt tcccaataat
    22621 atcgccaatt tcacgatcat taagaccgat gaagaaggac ataaaaatga tttcttgtct
    22681 ttgactaggt aatgctgcaa tcgcatttgc caactcctca ttggaaataa agattttcat
    22741 accaaggaca ataaaagtac cactactatt caaatttggt tccaaataat agaactctgt
    22801 acctaaccct ttttgttcca atatttccat tgatatttgc cgttggcgaa atctagcgta
    22861 cttcttttga atatttaacg cctcattgca gactactttc ttacaaaaac tgtcaaacgt
    22921 atgttgaatg tgggtttcct ctgagtcgcc cataatattc tccccctcaa aaaattcatt
    22981 caactcgttt tacttctatc cctttccatt attactacaa tctag
//
LOCUS       DACEKH010000036        22962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEKH010000036 DACEKH010000000
VERSION     DACEKH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.037"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            493..1200
                     /locus_tag="IW662_001345"
     CDS             493..1200
                     /locus_tag="IW662_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0866178.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1259..1903)
                     /locus_tag="IW662_001346"
     CDS             complement(1259..1903)
                     /locus_tag="IW662_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ0866179.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2030..2317
                     /locus_tag="IW662_001347"
     CDS             2030..2317
                     /locus_tag="IW662_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866180.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2425..2745
                     /locus_tag="IW662_001348"
     CDS             2425..2745
                     /locus_tag="IW662_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0866181.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2826..4139
                     /locus_tag="IW662_001349"
     CDS             2826..4139
                     /locus_tag="IW662_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0866182.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4136..4855
                     /locus_tag="IW662_001350"
     CDS             4136..4855
                     /locus_tag="IW662_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ0866183.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4983..5534
                     /gene="raiA"
                     /locus_tag="IW662_001351"
     CDS             4983..5534
                     /gene="raiA"
                     /locus_tag="IW662_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ0866184.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5756..8290
                     /gene="secA"
                     /locus_tag="IW662_001352"
     CDS             5756..8290
                     /gene="secA"
                     /locus_tag="IW662_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ0866185.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8416..9517
                     /gene="prfB"
                     /locus_tag="IW662_001353"
     CDS             join(8416..8478,8480..9517)
                     /gene="prfB"
                     /locus_tag="IW662_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ0866186.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9622..10308
                     /gene="ftsE"
                     /locus_tag="IW662_001354"
     CDS             9622..10308
                     /gene="ftsE"
                     /locus_tag="IW662_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ0866187.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10292..11185
                     /locus_tag="IW662_001355"
     CDS             10292..11185
                     /locus_tag="IW662_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0866188.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11337..12785
                     /gene="cls"
                     /locus_tag="IW662_001356"
     CDS             11337..12785
                     /gene="cls"
                     /locus_tag="IW662_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0866189.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13122..13979
                     /locus_tag="IW662_001357"
     CDS             13122..13979
                     /locus_tag="IW662_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ0866190.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14094..15014
                     /gene="pstC"
                     /locus_tag="IW662_001358"
     CDS             14094..15014
                     /gene="pstC"
                     /locus_tag="IW662_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ0866191.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15014..15898
                     /gene="pstA"
                     /locus_tag="IW662_001359"
     CDS             15014..15898
                     /gene="pstA"
                     /locus_tag="IW662_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ0866192.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15913..16719
                     /gene="pstB"
                     /locus_tag="IW662_001360"
     CDS             15913..16719
                     /gene="pstB"
                     /locus_tag="IW662_001360"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866193.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16735..17493
                     /locus_tag="IW662_001361"
     CDS             16735..17493
                     /locus_tag="IW662_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866194.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17506..18183
                     /gene="phoU"
                     /locus_tag="IW662_001362"
     CDS             17506..18183
                     /gene="phoU"
                     /locus_tag="IW662_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ0866195.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18406..19437
                     /locus_tag="IW662_001363"
     CDS             18406..19437
                     /locus_tag="IW662_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0866196.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19434..20201
                     /locus_tag="IW662_001364"
     CDS             19434..20201
                     /locus_tag="IW662_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0866197.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            20301..21869
                     /gene="liaX"
                     /locus_tag="IW662_001365"
     CDS             20301..21869
                     /gene="liaX"
                     /locus_tag="IW662_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ0866198.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22048..22353
                     /locus_tag="IW662_001366"
     CDS             22048..22353
                     /locus_tag="IW662_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ0866199.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF"
     gene            22353..22703
                     /locus_tag="IW662_001367"
     CDS             22353..22703
                     /locus_tag="IW662_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0866200.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            22841..>22962
                     /locus_tag="IW662_001368"
     CDS             22841..>22962
                     /locus_tag="IW662_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0866201.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNV"
BASE COUNT         7554 a         3957 c         4943 g         6508 t
ORIGIN      
        1 gttagttcga gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgaat
       61 taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa
      121 gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa
      181 gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat
      241 catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca
      301 ttttcatcga ttttagggtc ttttgttatt ttctaggttg aacatatcta aaaaattgtt
      361 tctgacaata ggagtaggga gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac
      421 ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga
      481 cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa
      541 aatgaaatcg aacagggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta
      601 gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat
      661 gaaggaatct tagaaagaaa gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa
      721 gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg
      781 tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta
      841 aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc
      901 cctatttgtt ttgaagttgc cagtgttcca caaaagctga tcgatggcta cagcaaagca
      961 gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca
     1021 gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta
     1081 aaacgaggag atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg
     1141 tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa
     1201 aaaacaggtg atccctgatt ccttattaga aataaaagag aatcagaatc acctgttttt
     1261 atatatgatc agttatcagt tgctcgtgat aagaaacaga accctcttca aattggactt
     1321 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
     1381 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
     1441 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
     1501 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg
     1561 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
     1621 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgccc gccgttccac
     1681 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
     1741 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
     1801 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
     1861 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
     1921 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
     1981 caattgctgt agtatgcgat aataaagaca aaggcaggtg gatctgggga tgcaggaacg
     2041 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
     2101 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
     2161 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
     2221 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
     2281 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
     2341 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcatagt
     2401 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
     2461 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
     2521 gactcggata ttttcgctgt gtccgcttct gacgcagata acaaccttgc aaataaagac
     2581 gtggatgtgt tgttattagg cccacaagtt cgctttatga aagctgattt tgagaaacgt
     2641 ctggaaccaa aaggcattcc tttagatgtc atcaatatgg ctgattatgg tatgatgaat
     2701 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
     2761 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
     2821 agaatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattgaa gcaatagact
     2881 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
     2941 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
     3001 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
     3061 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
     3121 ttactgaaaa acaaacggaa atctcaaaga gaatactttg ccattcagaa aaaagacatc
     3181 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
     3241 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
     3301 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
     3361 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
     3421 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
     3481 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
     3541 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
     3601 acaaacttgc tttacgcttt catcggcgtt tgcttcctga acccgtcttg gttttcatga
     3661 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
     3721 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
     3781 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
     3841 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gatatactgt
     3901 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
     3961 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
     4021 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
     4081 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
     4141 gtgcagttgc tgcagtaaag atatcttcca aaatttaaca ttcagcgaga ttattggtgt
     4201 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
     4261 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
     4321 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
     4381 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
     4441 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
     4501 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
     4561 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
     4621 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
     4681 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
     4741 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
     4801 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttggcga gatgaattag
     4861 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
     4921 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
     4981 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
     5041 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
     5101 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
     5161 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
     5221 tggttgttga taaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
     5281 cccgtgaaac gggcatgaat actgcagatg ctgctgcttt attcaatgaa gaggacgaaa
     5341 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
     5401 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
     5461 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
     5521 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
     5581 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
     5641 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
     5701 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
     5761 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
     5821 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
     5881 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
     5941 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccata
     6001 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
     6061 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
     6121 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
     6181 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
     6241 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
     6301 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
     6361 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
     6421 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
     6481 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
     6541 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
     6601 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
     6661 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
     6721 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
     6781 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
     6841 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
     6901 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
     6961 tcgtccagat ttactttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
     7021 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
     7081 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
     7141 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
     7201 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
     7261 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
     7321 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca caattctact tgtcattgga
     7381 agatgatttg atgaaacgat tcggttcaga acgaatcaaa gctttcttag atcggatgaa
     7441 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
     7501 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
     7561 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
     7621 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
     7681 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
     7741 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacatctca
     7801 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
     7861 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
     7921 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
     7981 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
     8041 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
     8101 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
     8161 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
     8221 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
     8281 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
     8341 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
     8401 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
     8461 aaggtcacaa gtttcaggag gtctctttga cttagatcag ctggaagaag acatcgcgga
     8521 agcagaagca aaaatggctg aacctggttt ttgggatgac agcgaagccg cacaaaaagt
     8581 gatcagcgaa aataacgcta ataaagaaac ctatgaccaa ttccattctc ttgccgaaga
     8641 actagaagaa atcgaattga tggtggagat gctgcaagaa gaacctgatg cagcaatgca
     8701 agaagaagca gaaacacgta tcaatgcatt agatgaaaaa atgaagacgt atgaattatc
     8761 tatgctgtta gacggacctt atgatcgaaa caatgcgatc gtcgaattgc atccaggggc
     8821 tggcggaacg gagtcccaag actggggcag catgctttta cgtatgtata cacgttgggc
     8881 agagcagcat ggctatcaag tagaaacact agattatcaa gcaggtgatg aagcagggat
     8941 caagagtgtg accctgctga ttaaaggaca taacgctttt ggttatctaa aatcagaaaa
     9001 aggtgttcat cgccttgtac ggatttcgcc ttttgactca gcgaaacgac gtcatacttc
     9061 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
     9121 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
     9181 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
     9241 acaacgctca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
     9301 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
     9361 aatcggctgg ggttcacaga tccgttctta tgttttccat ccctattcta tggtaaaaga
     9421 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
     9481 atttattgac ggctatttaa aaatgcatct acaataagaa ctcattgtta aattgaaata
     9541 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
     9601 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
     9661 ggtacgaccg ctatccgcaa tctctcgatt gtcatcgaac aaggagagtt tgtctatgtg
     9721 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
     9781 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
     9841 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
     9901 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgagat
     9961 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    10021 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatcgtg
    10081 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    10141 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    10201 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    10261 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    10321 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    10381 cagtgcggta acgatcacgt tgactttagt cggtgtattc atggcagtta tcatgaatgc
    10441 aactaaatta gctcaggata tagaaggaaa cgttgatgta tctgtattcg tagatatcgg
    10501 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    10561 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    10621 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    10681 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    10741 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    10801 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    10861 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    10921 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    10981 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    11041 caatccatca ttattacgtt cccattattc attgattcgt ccacaaaata tcgtttggaa
    11101 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    11161 tatgagacgt tttttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    11221 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    11281 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    11341 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    11401 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatggctg ctcgttttga
    11461 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    11521 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    11581 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    11641 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    11701 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    11761 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    11821 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    11881 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    11941 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    12001 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    12061 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    12121 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    12181 atacaaaaga agtaggctat tctcccaaat ttttcccgtc cattcacact actggtgata
    12241 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    12301 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    12361 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    12421 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    12481 cagagctgat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    12541 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    12601 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    12661 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    12721 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    12781 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    12841 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    12901 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    12961 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    13021 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    13081 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    13141 tcttaccaat aataggatta gccattcttt tatcaggctg ttcaaagtgg attgatcgag
    13201 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    13261 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    13321 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    13381 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    13441 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    13501 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    13561 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    13621 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    13681 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    13741 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    13801 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    13861 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    13921 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    13981 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    14041 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    14101 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    14161 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    14221 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    14281 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    14341 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    14401 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    14461 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    14521 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gacagggttt ggtatcttag
    14581 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    14641 cattaaaggc agtaccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    14701 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    14761 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    14821 cagcattgat gccaacagga cttactactc ctgcttctac attgacgtcc atcttaacaa
    14881 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    14941 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    15001 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    15061 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    15121 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggtgg
    15181 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    15241 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    15301 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    15361 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    15421 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    15481 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    15541 aacagtgatg aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    15601 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    15661 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    15721 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    15781 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg atcattgctg ttttactgtt
    15841 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    15901 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    15961 gagaaagaag tagcactata tacggatgat atacatgtct ggtatggcga caatgaagca
    16021 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    16081 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    16141 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    16201 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    16261 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    16321 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    16381 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgcattgct
    16441 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    16501 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    16561 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    16621 tatctaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    16681 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    16741 aaagaaatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    16801 gggatcgatc taagtttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    16861 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    16921 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    16981 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    17041 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    17101 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    17161 cataaaagtg ctttatcttt atccggcggg cagcagcagc gtgtatgtat cgcacgtgtt
    17221 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    17281 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    17341 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    17401 ggtcacttga tcgaatacaa cgatacgaag aagatattca tgaatccaga aaaacaagag
    17461 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    17521 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    17581 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    17641 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    17701 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    17761 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    17821 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    17881 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    17941 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    18001 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    18061 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    18121 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    18181 taaactgaag ctgtgattat gccgtttgtt tatgaaaaga ggttgtgaaa aagctgttct
    18241 acatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg
    18301 acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa
    18361 aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt
    18421 agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagcc
    18481 gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga ggatcatcaa
    18541 ttgattacag gaaaactgat gaactcggat caaaaaggag aatcgattta tttatcggtt
    18601 ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc
    18661 caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc
    18721 ttcacaagta tcttcattgg cgccatgttg cttattggcg gaaaacaggg tctatttgct
    18781 ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa
    18841 acagaaggga tttctctatt aaataaatct atcctgttcg tcgttatagg gatgattatt
    18901 agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt
    18961 acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa
    19021 ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct
    19081 agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca
    19141 ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga
    19201 aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc
    19261 agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcttt caaagaaacg
    19321 atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta
    19381 ttatcgatcc ccatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg
    19441 tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag
    19501 gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg
    19561 gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg
    19621 tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta
    19681 tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt ttagcgatcc
    19741 aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg
    19801 attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg
    19861 cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt
    19921 tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa
    19981 tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt
    20041 tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg
    20101 ctattcttac cacattgtcc gcactaattg ttcttccttt aacctgcgtg actacagcat
    20161 atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct
    20221 atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt
    20281 tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag
    20341 gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg
    20401 aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagataagg
    20461 caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc
    20521 caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa
    20581 ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata
    20641 aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag
    20701 gcgagttgac agaagaagaa ttagcacagc gtcaggcgtt agaggcagaa atcaaagagt
    20761 tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag
    20821 atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc
    20881 cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag
    20941 aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag
    21001 tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga
    21061 aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag
    21121 caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga
    21181 tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga
    21241 ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc
    21301 ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca
    21361 acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca
    21421 tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg
    21481 atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat
    21541 ttggtgcgac agcagaaaat ctttctgtgt ctttagtaaa tggtgatgta cgtcttacga
    21601 ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg
    21661 ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaata
    21721 gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc
    21781 aattccgtcg tgtttcagat gatgagatcg cgcaagtgca actttcaacg acaacaggaa
    21841 gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag
    21901 caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc
    21961 gataggctat ctcttgaaca ccgattaaga agaaggttgt cacaacgatt ttcttcattt
    22021 aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc caataaaaat
    22081 gttgttttga caggtacttt agcagggatt ggggaatata tcgggattga tcccactgtt
    22141 atccgtgtgc tttatgtatt cctaagctta gttgcttttg gaagtccaat cattttatac
    22201 attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta tggacataac
    22261 aatgcatatt atgaaaaaaa caattatcgt gaacaaacaa aaaaacgcaa agaagcggaa
    22321 aaaatcgatg atgacgaatg gagtgacttt taatgtcgta ctttcagcgt attttggtta
    22381 atacgttaac attcatatcg ttggctgttc tttttcctgc gatggtccat gtgagcagta
    22441 tttggacagc cgtccttgcc agcttcgtat tatctgtgtt gaacatgttt gtcaaaccga
    22501 ttctgacgat cctgtccctg ccctttacat tagtcacatt gggattgttt acgtttgtta
    22561 tcaatgcagc gattcttaaa atgacttcct tttttgtagg agcagaacat tttagttttt
    22621 cttcatttgg tgcagcgtta ttcgtagcta tcttgatgtc acttgtcaat gtaatcgtta
    22681 gtgaacataa cagcagaaag taaaaaaagc tgtgccgaga tctcggcgca gcttttttgt
    22741 ttatcgcgaa ttgtaaaatt aagttagaaa aataaaaagg catttatggt acactcaaat
    22801 tgtatttccg acgaaagaaa acaaaggagt gtaaccataa atgacctaca aacatcttac
    22861 catagacgaa ctgacaatga tagaatcata ttatcttcaa cataataagc cggttgaaat
    22921 cgctaaccga atgggacgtg ctatacaaac tatttataat gt
//
LOCUS       DACEKH010000037        22791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEKH010000037 DACEKH010000000
VERSION     DACEKH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.038"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(91..2022)
                     /locus_tag="IW662_001369"
     CDS             complement(91..2022)
                     /locus_tag="IW662_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0866202.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(2040..2954)
                     /gene="pfkB"
                     /locus_tag="IW662_001370"
     CDS             complement(2040..2954)
                     /gene="pfkB"
                     /locus_tag="IW662_001370"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0866203.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2951..3703)
                     /locus_tag="IW662_001371"
     CDS             complement(2951..3703)
                     /locus_tag="IW662_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0866204.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3862..4071)
                     /locus_tag="IW662_001372"
     CDS             complement(3862..4071)
                     /locus_tag="IW662_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866205.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4082..5410)
                     /locus_tag="IW662_001373"
     CDS             complement(4082..5410)
                     /locus_tag="IW662_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866206.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(5775..6362)
                     /locus_tag="IW662_001374"
     CDS             complement(5775..6362)
                     /locus_tag="IW662_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ0866207.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6445..7695)
                     /gene="clpX"
                     /locus_tag="IW662_001375"
     CDS             complement(6445..7695)
                     /gene="clpX"
                     /locus_tag="IW662_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ0866208.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7942..9231)
                     /locus_tag="IW662_001376"
     CDS             complement(7942..9231)
                     /locus_tag="IW662_001376"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ0866209.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9385..10317)
                     /locus_tag="IW662_001377"
     CDS             complement(9385..10317)
                     /locus_tag="IW662_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866210.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10474..11133)
                     /locus_tag="IW662_001378"
     CDS             complement(10474..11133)
                     /locus_tag="IW662_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0866211.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQMIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11136..12161)
                     /gene="sppA"
                     /locus_tag="IW662_001379"
     CDS             complement(11136..12161)
                     /gene="sppA"
                     /locus_tag="IW662_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0866212.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12174..12464)
                     /locus_tag="IW662_001380"
     CDS             complement(12174..12464)
                     /locus_tag="IW662_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866213.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12873..14678
                     /gene="glmS"
                     /locus_tag="IW662_001381"
     CDS             12873..14678
                     /gene="glmS"
                     /locus_tag="IW662_001381"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ0866214.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14750..16105)
                     /locus_tag="IW662_001382"
     CDS             complement(14750..16105)
                     /locus_tag="IW662_001382"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ0866215.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16129..17298)
                     /locus_tag="IW662_001383"
     CDS             complement(16129..17298)
                     /locus_tag="IW662_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866216.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17295..18182)
                     /locus_tag="IW662_001384"
     CDS             complement(17295..18182)
                     /locus_tag="IW662_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ0866217.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18371..22063)
                     /gene="nifJ"
                     /locus_tag="IW662_001385"
     CDS             complement(18371..22063)
                     /gene="nifJ"
                     /locus_tag="IW662_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ0866218.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22262..22783
                     /locus_tag="IW662_001386"
     CDS             22262..22783
                     /locus_tag="IW662_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0866219.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6356 a         4949 c         4047 g         7439 t
ORIGIN      
        1 tacaacaact attcatctac actacttgat tctatatgat tctataaaaa aagaagaggc
       61 tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag gagcttttct
      121 caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca ccaaatacat
      181 gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca gtaatttgat
      241 tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac ttggaatggc
      301 acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg atagtcccat
      361 cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct taaacaaacg
      421 tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag aagccatcac
      481 gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca cataagcggc
      541 tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg caccaagtaa
      601 agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt
      661 catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa acactggata
      721 aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata atttacgtaa
      781 aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga atcctgcacc
      841 gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat
      901 acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat
      961 ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt gatcatgtgg
     1021 cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc caccaataac
     1081 gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt gtccgatagt
     1141 accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga atactggtgc
     1201 agtaccacta agggctagat tgatcaactc ttctggttta cggataccat cactgacagg
     1261 acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt ctgctgcaac
     1321 gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga ttccttctga
     1381 accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct tcaaagcatc
     1441 ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa caacgaattt
     1501 tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt tttcttctgc
     1561 ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg tatttgcttc
     1621 ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg
     1681 caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat aagttggctg
     1741 tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa ctgttgcttc
     1801 tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt
     1861 ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa tccggccggc
     1921 atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc
     1981 catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc tccttgaaac
     2041 taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc gcgagctgcc
     2101 aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa ggcttcgatt
     2161 ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc ccctgcacct
     2221 actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg ttccgtgaag
     2281 agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc agctagtaat
     2341 tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta aagctcagct
     2401 aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt ttttaggtca
     2461 tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc aatcagttct
     2521 tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac gatatctcct
     2581 tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga aatagatggg
     2641 cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat tcttgtatct
     2701 tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt cgaaataaat
     2761 tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa
     2821 atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt cattcggttc
     2881 aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg attaagtgtt
     2941 actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat tcattaacga
     3001 attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca acagaggtga
     3061 aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca
     3121 tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct tgttcaagat
     3181 gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt tgttctatcg
     3241 cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa ataatggtag
     3301 caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact actgtgatct
     3361 gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct aaataaatga
     3421 catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt
     3481 ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct ttcttagctc
     3541 cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca cgtcttatcg
     3601 ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt ttctgctgtt
     3661 caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg atcacctcaa
     3721 tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa acgttcataa
     3781 aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt tttttatttt
     3841 tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt ttttcatata
     3901 aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca tcccgccaaa
     3961 catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa agagacagac
     4021 ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca ttttcttcac
     4081 ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa aattgcacac
     4141 gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt ttctttattc
     4201 gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat tctgacaggg
     4261 attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt tcatctgtta
     4321 cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca ttatccaaag
     4381 caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg tcaacccaag
     4441 gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa tctccttgtt
     4501 ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca ggttgcattt
     4561 gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat tcaacgaatg
     4621 gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca agctggtccc
     4681 taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg tctcgataaa
     4741 gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt tggatgtcga
     4801 gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt tcccaacacg
     4861 aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg caatcgacta
     4921 ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc tttttctcaa
     4981 acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta taagctcgca
     5041 tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga atcaaaaatt
     5101 gcttttcttt gaaaacagag atagacaata cattttttat caataattga ttatcttcca
     5161 agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg tctcttaccg
     5221 atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct tgttgtgtga
     5281 aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt
     5341 cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat aatttatctt
     5401 cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa ccaatgataa
     5461 agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga tagactaagt
     5521 aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag ataatttaga
     5581 taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat
     5641 cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta
     5701 agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg actggtaagt
     5761 cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc ttcttctttt
     5821 ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa atccaacgct
     5881 tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc tgccttagtg
     5941 gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg gatatcttct
     6001 tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg gtctctagtc
     6061 gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga tacttttgca
     6121 tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata aaaattcaat
     6181 gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc aatcaatgtg
     6241 ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc tggcaattgt
     6301 gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg atggactttc
     6361 atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat
     6421 gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt tatagattac
     6481 tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt cgatgttttc
     6541 atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg aacgaagtcc
     6601 tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg cttcaggttc
     6661 gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga ctagcgcatt
     6721 tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta aagctgccat
     6781 aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat cttcaggaat
     6841 gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac caaatccaat
     6901 cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg caccacctac
     6961 gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat gtttacgtcc
     7021 gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg cttgttggac
     7081 accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg caattttatc
     7141 gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat cagcagattg
     7201 aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt ctgtcaaact
     7261 tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt gtgctaaaaa
     7321 tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca gttcaacatc
     7381 atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata cggcaacagc
     7441 taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt caagaatttc
     7501 tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt cttcatcaat
     7561 gatttcttta cataggtcga tacattcatt acagatgtag acccctggac cagctacgat
     7621 ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct cattgctact
     7681 tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag aaactatcaa
     7741 agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag tgtttaacaa
     7801 aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa ggaactgtga
     7861 caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta gcttcttcag
     7921 aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg tgatcaattc
     7981 gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca cgcgtttgat
     8041 ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat tgatttcttc
     8101 ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat ttgttcttgt
     8161 gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag aaccagttaa
     8221 ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga attcgtccat
     8281 tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc
     8341 aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc
     8401 tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt cgtcatcaac
     8461 atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt catggattgt
     8521 cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt ctgggaaagt
     8581 tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt caaaacctgg
     8641 aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc catcaaacgc
     8701 ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt cagctgcagc
     8761 atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac gtgcatccac
     8821 gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat attgtcctaa
     8881 ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac ctttttccat
     8941 gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt tgacagcagc
     9001 ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt cttcaccgta
     9061 catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta cgttcaagct
     9121 ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt
     9181 aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca tttaattccc
     9241 tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct ttaaatagta
     9301 cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt ttcataacat
     9361 ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca aaattttttc
     9421 tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg tttttcctgt
     9481 acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg tatcatagac
     9541 aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac taagcagcgc
     9601 atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg cctgctgctg
     9661 cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg tcaaatagaa
     9721 aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc ttgctaaaat
     9781 atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat gataaggaag
     9841 ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag gaagttcctc
     9901 cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata attcttctgc
     9961 aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg tacttgctat
    10021 caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat aaaattcagc
    10081 aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca atgcttcttt
    10141 tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa agctttcttt
    10201 caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt ttgctagtgc
    10261 ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg gcttcacgca
    10321 gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg
    10381 acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga cttttagaag
    10441 gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct ggttgaacgc
    10501 tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca gatgttgctt
    10561 tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt ttagaatgaa
    10621 ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt catcaaagct
    10681 gatgactcga ataccaaaga tcattttccc aatcatttgg ccatgattga atttcgtcaa
    10741 taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac tcagataaga
    10801 atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac gcgtaatagc
    10861 tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa
    10921 atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat actttttcca
    10981 tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa aagagctcag
    11041 taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct tatctttttc
    11101 ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta ataatacatt
    11161 gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg actagtctca
    11221 gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact agcaaaccca
    11281 gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc ctttcgtata
    11341 gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc ttttgcttgg
    11401 acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga tttattccgt
    11461 ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc
    11521 gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt
    11581 ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt caatcctgac
    11641 atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt atctgcttga
    11701 gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata cattgggata
    11761 tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc gtagacaccg
    11821 ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc ttcaatcgcc
    11881 ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa acctgaagac
    11941 cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt actgtctccc
    12001 tcttccaaca catttggagc tagttcttca tccccatata accaactgtt caatccctca
    12061 atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat
    12121 cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc tttctaatac
    12181 aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg ctctgctttt
    12241 tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac cgtaaactca
    12301 cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc ccagccagta
    12361 tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt ggacaattgt
    12421 ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac tcctcctcta
    12481 tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa acccattatt
    12541 ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta ttttttattc
    12601 tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc cagataaaaa
    12661 acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa acagatgacg
    12721 aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc tacagaaaat
    12781 ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg aagcattcat
    12841 acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga atgattggat
    12901 taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac cgtggttatg
    12961 attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa gcacaaggca
    13021 gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata ggtattggtc
    13081 atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca catacttccc
    13141 aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa
    13201 ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt atcgctcatc
    13261 taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa gctttgcggg
    13321 tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat gttatttacg
    13381 tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat gtgattgcca
    13441 gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa gatgaagaga
    13501 tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac atcgtagaac
    13561 gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc tatccttttt
    13621 atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct caaaaatacg
    13681 taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg tctgatagcg
    13741 atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca ggtaaacaga
    13801 ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa tttggctata
    13861 acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt ggcgaaacag
    13921 cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca cttacgatta
    13981 caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta ttgcatgctg
    14041 gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct gtactaactt
    14101 tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta ttcgatatca
    14161 ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa aaagattatt
    14221 tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac attggacgtg
    14281 gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc agttatatcc
    14341 aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg atcgaagaag
    14401 gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact cgaggaaacc
    14461 tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag ggattagaaa
    14521 aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact ctaaccacag
    14581 tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc gacgttgaca
    14641 aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag gaccagtgtc
    14701 agacatctga cactggtcct tgttctttta ttttttatga aaatatctct taatccagtc
    14761 cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact ttctcatcag
    14821 taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga actaggattc
    14881 ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt gccggaacat
    14941 ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga tagattgtta
    15001 cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc agtaactgaa
    15061 ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg tgcccagact
    15121 gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa
    15181 cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat cctaagttac
    15241 tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc aagtatttag
    15301 cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc accgcaataa
    15361 tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca acgaccatct
    15421 cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac ccgtctggat
    15481 ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta acacttgttg
    15541 aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca ccgttgatcg
    15601 tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct acagttccca
    15661 atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat gcttcaattt
    15721 ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg attccattat
    15781 cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg
    15841 tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg atacccaccg
    15901 ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt gtatcccgcc
    15961 ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca taacctccgc
    16021 accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca ccgcgtacgc
    16081 cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt aattttctga
    16141 ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt ctgtacttgt
    16201 ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg atggttccgt
    16261 tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg tccgactgct
    16321 cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca cactaactga
    16381 ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag ttgttcgatg
    16441 attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg caagcaaatc
    16501 gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc tttctacccc
    16561 ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg gcttgatcga
    16621 atcagctctc actgttactt gcactggatc agagacgata cttaatactt cccctgctgc
    16681 attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca tcgaagcgtc
    16741 cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag tgatagagac
    16801 ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga acgttgaagg
    16861 aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta ctgtaatagt
    16921 cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta gcggaacttc
    16981 atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc
    17041 gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag tcactgcatt
    17101 ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg gcacccccgt
    17161 aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact ggccatttgc
    17221 attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg
    17281 tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag gaaagattga
    17341 atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg caagattgcc
    17401 aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat gctgacgtcc
    17461 ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat accgacagct
    17521 gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag aggaagatag
    17581 gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg cagcggtgta
    17641 ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa agcaatccct
    17701 gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc accgattttt
    17761 cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc atcttcctgc
    17821 tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg
    17881 atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac ttggttcatc
    17941 agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac aataaaaata
    18001 gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat cagtttgtat
    18061 acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag agagaacaag
    18121 tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac
    18181 ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg ttttaatttt
    18241 agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag aaaattgcaa
    18301 gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat tattcattat
    18361 attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt ttacgtcgtt
    18421 ctgctctggc agctcttcta gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt
    18481 cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt ttcttgattt
    18541 tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag
    18601 tcttttcaaa taaagctttt gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa
    18661 cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag tccaaagtca
    18721 ttgggttctt gtttttttcg accattgtag ggttatatcg atataatgac caataaccag
    18781 aagaaacagc ttcttttgct tctttaagcg attctttcat tccgccaagc aagccatgtg
    18841 tgatacaagg cgtataggca ataatcagag aaggaccagg atacttttct gcttcttcaa
    18901 atgccttgat cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca taaacatttc
    18961 catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca ctagccgaaa
    19021 atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta ttggaataga
    19081 cttcgttatc caaaacaagg atattcacgt ctgctccgct ggcaagtaca tgatcgatcc
    19141 cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg ctgtttttta
    19201 cgaatagatc ctttctcttg tatatatcct ccagttcagg gaaaattgat cgttcgcttt
    19261 cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcactctca aacatgtgta
    19321 ctaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat acttctttga
    19381 ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca taaccaaatt
    19441 cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat tcattggtcg
    19501 tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc gcattcgcga
    19561 tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt tcgccacagc
    19621 ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac aagacagtat
    19681 tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc caattcacag
    19741 cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt gcgggacaag
    19801 cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata cggtatttta
    19861 cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct tccatttcct
    19921 cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca cattcattac
    19981 acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc cgcttttcca
    20041 tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca agtgtaccaa
    20101 cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt atataaccag
    20161 tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct gggatcgcga
    20221 cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct acgatagcca
    20281 ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc acctcatcaa
    20341 aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc
    20401 ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta atgttatttt
    20461 cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg tcgctccaaa
    20521 gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca tagctatgca
    20581 ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac gctgaacgta
    20641 tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt ttcgaatcat
    20701 aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg atagctgatt
    20761 tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg aatgtttgcg
    20821 gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca tcatccacga
    20881 tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg
    20941 ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca ccaataacag
    21001 ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga ggttctccac
    21061 ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta tgaggcaact
    21121 tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa aaacctgttt
    21181 tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca actgatccca
    21241 tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag tcataagaag
    21301 ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt aatttatcat
    21361 aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg ttcgtaccac
    21421 taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat ttctcctgat
    21481 tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc tcgtgactcg
    21541 tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc ttcaatagaa gcaagatggg
    21601 caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata gcaaatcctg
    21661 tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca tttgtcgtca
    21721 ctgcccgact agcaacgtgg aaaacacttg gcagtagttc cccagcaatc ttgtacatat
    21781 tcggtatcat caaaagcagc ccttgtgaag cggtatatgt agttgtcagt gcaccagttt
    21841 tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct actactttta
    21901 ctggttgacc gaatacgttt tcttttcctg ctgctgacca ttgatctact aattcagcca
    21961 tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca taagaaatat
    22021 atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat catccttgtt
    22081 catattctct cttattatct gttccattat aacgtaaaac atgccatttt tttcttttat
    22141 ttataaaaat attttacatg tatctatttg aattggtcat ctaattttta gacaaacagt
    22201 ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga ggtgttttaa
    22261 catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa tcctttatgg
    22321 ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc cgtattgccg
    22381 acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg ggcaattgca
    22441 gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga acgtgatgca
    22501 ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac aaatattcga
    22561 tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg cagtagataa
    22621 actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca acgaagctga
    22681 gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct ttgatgaatc
    22741 tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata a
//
LOCUS       DACEKH010000038        22527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEKH010000038 DACEKH010000000
VERSION     DACEKH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.039"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            91..2736
                     /gene="polA"
                     /locus_tag="IW662_001387"
     CDS             91..2736
                     /gene="polA"
                     /locus_tag="IW662_001387"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ0866220.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            2781..3617
                     /gene="mutM"
                     /locus_tag="IW662_001388"
     CDS             2781..3617
                     /gene="mutM"
                     /locus_tag="IW662_001388"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ0866221.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            3581..4210
                     /locus_tag="IW662_001389"
     CDS             3581..4210
                     /locus_tag="IW662_001389"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ0866222.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            4262..4594
                     /locus_tag="IW662_001390"
     CDS             4262..4594
                     /locus_tag="IW662_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0866223.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            4906..5397
                     /gene="nrdR"
                     /locus_tag="IW662_001391"
     CDS             4906..5397
                     /gene="nrdR"
                     /locus_tag="IW662_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ0866224.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            5420..6793
                     /locus_tag="IW662_001392"
     CDS             5420..6793
                     /locus_tag="IW662_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ0866225.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            6793..7719
                     /gene="dnaI"
                     /locus_tag="IW662_001393"
     CDS             6793..7719
                     /gene="dnaI"
                     /locus_tag="IW662_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ0866226.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            7848..8288
                     /locus_tag="IW662_001394"
     CDS             7848..8288
                     /locus_tag="IW662_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0866227.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            8432..9133
                     /locus_tag="IW662_001395"
     CDS             8432..9133
                     /locus_tag="IW662_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ0866228.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            9130..10251
                     /locus_tag="IW662_001396"
     CDS             9130..10251
                     /locus_tag="IW662_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ0866229.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            10266..11498
                     /gene="pepT"
                     /locus_tag="IW662_001397"
     CDS             10266..11498
                     /gene="pepT"
                     /locus_tag="IW662_001397"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ0866230.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            11630..12538
                     /locus_tag="IW662_001398"
     CDS             11630..12538
                     /locus_tag="IW662_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ0866231.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            12573..12845
                     /locus_tag="IW662_001399"
     CDS             12573..12845
                     /locus_tag="IW662_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ0866232.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            12856..13902
                     /locus_tag="IW662_001400"
     CDS             12856..13902
                     /locus_tag="IW662_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ0866233.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            14153..16762
                     /gene="clpB"
                     /locus_tag="IW662_001401"
     CDS             14153..16762
                     /gene="clpB"
                     /locus_tag="IW662_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ0866234.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(16913..17584)
                     /locus_tag="IW662_001402"
     CDS             complement(16913..17584)
                     /locus_tag="IW662_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866235.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(17577..18071)
                     /locus_tag="IW662_001403"
     CDS             complement(17577..18071)
                     /locus_tag="IW662_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0866236.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(18091..19311)
                     /locus_tag="IW662_001404"
     CDS             complement(18091..19311)
                     /locus_tag="IW662_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0866237.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            19458..21293
                     /gene="lepA"
                     /locus_tag="IW662_001405"
     CDS             19458..21293
                     /gene="lepA"
                     /locus_tag="IW662_001405"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0866238.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(21387..22514)
                     /locus_tag="IW662_001406"
     CDS             complement(21387..22514)
                     /locus_tag="IW662_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0866239.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         7524 a         4067 c         4778 g         6158 t
ORIGIN      
        1 attatttggt atggtcccgc tttttctttt cgcattccgc aggattcttt gttagaatga
       61 ataagaactt tacagtaagg agttaagaag atgacaaaaa ataaattatt actcgttgat
      121 gggaacagtg tggcttttcg cgcattcttt gcgcttcaca attctcttga acgtttcaaa
      181 aatagaaacg gtcttcatac aaatgcaatc tatgcattca atacgatgtt tgaaaatgtt
      241 atgcaaaaag agcaaccgac tcatgttctg gttgcgttcg atgcaggaaa aacgactttt
      301 cggacagaaa tgtatgcgga gtacaaaggt ggaagatcca aaacacctgg cgaattcaaa
      361 gaacaaatgc catatatacg tgatttgttg acaggactag gggtacagta ttacgaattg
      421 cccaattatg aagcagatga tattatcggt actttggcag aaaaagtaga caaagatcaa
      481 tttgatgtcg tcgtgctttc aggtgatcgg gatttgactc aattagctag taaagaagta
      541 aaagtggata tcactgtaaa aggtgtcagc gatatcgaaa gctatacacc agaacatgta
      601 gcggaaaaat atgatgggtt gacgccaaaa cagattattg acatgaaggg actagccgga
      661 gatgcctctg acaatatccc cggtgtgact aaaatcggtg aaaaaacagc tatcaagcta
      721 ttgaaacaat atggctcagt cgaaggaatt tatgaacata tcgatgaaat gaagcaaagc
      781 aaaatgaaag aaaatctgat aaatgacaaa gagcaggctt tcttatcgaa aaaattggcg
      841 accatcaata cttcagcgcc tgtggatgtc tctatcgact cattgaagta tgaagggaaa
      901 aatctcgata aactcgttcc gttttataaa gaaatggatt tcaatcagtt cctttctaaa
      961 ttgaatatcg tagaagaaga agtaaaaatg gatgatattt tgtttgaagt cgttcatgaa
     1021 ttcaaagagg aaatgtttac tacggatatg gcgttgtacg tggaaatgat gggcgacaat
     1081 tatcatacag aggaaatcgt cggagtagcg tgggggacag aaaagaaaat ttatgtcacg
     1141 aacgatctat ctgtattcga aaaccgagcg ttccattctt ggatcactga tccgactcgg
     1201 ctgaaaaaag tatatgatgc gaaacgaact tatgttgctt taaaccgcta tactggaaaa
     1261 acgaaaggaa tcgattttga tgtcttgcta ggtgcatacc ttctcgatac gaatgaaaaa
     1321 agcacagata tcgaaggaat agctgctcat tacggataca acgatatcca atcagatgaa
     1381 gctgtatacg gcaaaggggc gaaaaaaggt ttaccagaag aagaggaact cttttttgct
     1441 catttagcgc gaaaagtagc tgcaatcaat gcgttgacac caaaattgag ccaagaactg
     1501 gcagataaaa accaagaaga tcttttccgg aaaatggaac tgccattgtc tatcattttg
     1561 gcagagatgg aaataagcgg gatcaaagta gatgcgactc gcttacaaga aatgaagggc
     1621 gagttctctg ctcgcttaag agagatcgaa caaaaaatct acgaagaagc cggcgaagaa
     1681 ttcaatttga attcaccaaa gcaattagga gtgatcttat ttgaaaaaat gggattaccc
     1741 gttatcaaaa aaacaaaaac tggatattcc acagccgtag atgtactgga acagcttcgt
     1801 gaacaagcac caatcgtaga ggatatcctc acctatcgtc aaattgcaaa aattcaatct
     1861 acctatgtcg agggactgct gaaagtgatc caatcagatg gaaaagtgca tacccgttat
     1921 gtccagacat tgacccaaac aggacgatta agctctgttg atcctaattt gcagaacatc
     1981 ccgattcgat tagatgaagg gagaaagata cgtcaggcat ttgttcctag agaaaaagac
     2041 tggctgattt attcttctga ctattcacaa atcgagttac gcgtacttgc gcatatctca
     2101 aatgatgaac atttgaaagc tgccttccta gaaggtcaag atatccatgc cagcacagcg
     2161 atgcgtgtat ttggaatcga aaaggcagag gatgtcacac caaatatgcg taggcaggca
     2221 aaagcagtga attttggtat cgtgtatggc atctctgatt acggactttc acaaaatcta
     2281 ggaatcagcc gaaaagcagc acagcaatac atcgatacgt actttgaaaa atacccaggt
     2341 gtcaaagaat atatggaaag aatcgtacgc gaagcaaaag accaaggcta cgtcgaaacg
     2401 ctgtatcacc gccgtcgata tctgccagac atcaattcaa gaaattacaa tatccgttct
     2461 tttgcggaac gaacagctat caatacacca atccaaggaa gtgctgctga tattttgaaa
     2521 atcgcgatga tcgaattgga taaacgatta aaagaaacag gtcttcaagc tacgatgctg
     2581 ttacaagttc atgatgaact tgtcttcgag gtaccggaaa aagaacttga atcattagat
     2641 aaattggtga aggaagttat ggaacaagct gtttctcttc atgtaccact gatcactgac
     2701 agcagttggg gaaaaacgtg gtacgaagca aaataaacat tgtttaagac agagcaatat
     2761 aatgaaggtg aaaagaaaaa atgccagaat taccagaagt agaaaccgtt cgtaaaggat
     2821 tggaacggtt agtagtagga aaaacgattc aaaaagttca ggtcttgtgg cccaagatca
     2881 tcgagcaacc agagacaccc atctttgagg cttctcttgt tggcgaaacc atccaatcca
     2941 ttggacgtag agggaagttt ttaatttttc atctcgatca ttgtgaactg atctcacact
     3001 tgcgaatgga aggaaaatat caatttacga aagaaaatac accaatcgat aaacatacgc
     3061 atgtactttt cttttttgag gatggaagcc aacttcgcta caacgatgta cgaaaatttg
     3121 gtcgtatgac aatcgtcgaa aaaggagcaa gtgcaactta ccgaggaatc atgaaattgg
     3181 gacctgagcc tttgccagat agcttcttat tagcagattt cgcaaatgga ctgaaaaagt
     3241 cccacaaagc aatcaagcca ttgctgctag accaacgtct agtgactggt ctaggtaata
     3301 tttatgtaga tgaagcatta tgggaagcga agatccatcc agaacaaccg gcgaacacac
     3361 tgcgccccaa agaggtagaa caattacgtc tatcgatcat tgatgttttg gatcgtgcaa
     3421 tagaagccgg aggtaccacg attcgcagtt atttgaatgc attaggagaa tcaggcggat
     3481 ttcaagtagc actgcatgtc tatcagcaaa ctggcaaacc atgtatacgt tgtggaacgc
     3541 cgatcgtgaa aacaaaagtg gcacaaaggg ggacgcacta ttgtccgaat tgtcaaaggc
     3601 taaaaatcgc aaagtaggat ttgttttagg tttgacgggt ggaatagcta ccggtaaaag
     3661 tacggcagcc aaagtctttc agtctcatgg atttcctttg atcgatggcg atgtgattgc
     3721 cagagaaact gttgagcctg gaacgcttgc tctgaggaaa attgtatcag tgtttggcca
     3781 agaggtattg cagcctgatg ggcaactaga tcgagggaaa cttgggatga ttgtttttcc
     3841 atcaaaggaa aaacggcaaa aacttgatca gctattagat ccgtttatcc gaaaagcgat
     3901 aaaagatcaa atcgcctctc taagttccaa tcatccttta gtaattgtag atatcccatt
     3961 gctctacgaa ggtcattatg accactatat ggatgcagta gctgtcgttt atactacgcc
     4021 agaaactcag cttcagcgtt tgatgaaacg taatcaactg acgctagaac aagcgcagca
     4081 gagaattagt agtcaacttc caatcgaaga aaaaaagcag cgtgcggata ttttgttcga
     4141 caacaatgga acaaaagaaa aacttgtggg acaaatcgaa aattggcttt ttgctaatcg
     4201 ctttctttga gtatctgtgt caattttgct atcctgagta tatagaaagt gagggatagc
     4261 gatgggatca ttaaaaaatc aaaaggataa agtcgtaggt aacgcaaaag aaaaaatcgg
     4321 cgagatgctc aataaagaaa atctaatcga tgaaggtcgt acgcaatcag aaacagcaga
     4381 agaaaaagaa aagcagtacc aagcaagaat cgatgaaata gaagaccagc aggcagaaga
     4441 agaagcgaag cggcaagatt tgactcaaga acgaaaaaat ccgacacctg atcgtagtaa
     4501 aacaccgctc ccagaaagtg accacatttc cgatgagcaa aaaatcgagg aacagcgaat
     4561 gaaacactat acaggaatcg aagagaagtt ataaaatagt aaaaaatcga gatgtgactt
     4621 gaagcagatg agaaggtgtt catctgcttc aagttttttg tgctagatga ctaaatcagc
     4681 ttttgtgtaa gttatttctc agtaatcaag gtgatcagta ttggaaaatt gaaatttagt
     4741 aaggacaggg gacacaatta cgcatgaaag tagaaaaaat tgttcttata tccgtgtttt
     4801 ttgggtagga cagtcgcgtt tgtgatttgt ttcgtgttat aatacaacta gttgtaaaga
     4861 aagacacatc ctttatataa aatgcgatgc aagaggtgag acaaaatgag atgtcccaga
     4921 tgtcaacata ataattcacg agtaatcgac agccgtcaag cagatgatgg acgtgcgatc
     4981 cgtcgccgtc gcgaatgtga gaattgttcg ttccgattca ctacatttga acgaattgaa
     5041 gctgctccgt tattagtaat caagaaaaac ggggaacgcg aagagttcaa tcgcgataaa
     5101 attttaagag gactcatccg ttcagcagaa aaaagacctg tagctatgga acaaatggaa
     5161 caaatcgtca accatgtcga aaaccgcgta cgcagtctcg gagaaaacga agtatcgacg
     5221 aatctaattg gtgaatatgt gatggaagat ttagttgatt tagacgaaat cgcttatatt
     5281 cgttttgcaa gtgtttatcg ccagttcaaa gatatgtctg ttttcttaaa agagttgcaa
     5341 gatatcgtgg ataaagcaaa agaaaataat aaagagaata ataaagaaaa caattaagaa
     5401 acaaaaagga ggcatttcct tggaaaatgc ttggaaagag cttcaaccaa aaaatattta
     5461 ccaaggaaga aaagcggctc cgctgagaga agacagcaag aacgccttgc tgtatcttta
     5521 tcagccacta atcggcggag aagccttgag tatctatttc accctgttat cagaaatctc
     5581 tcaagaatct ggcaaaggtc ctgaagggat gcatgcagat ctgcttagta gtctaggctg
     5641 cggtatcccg caattttatg atgcaagaaa aaagctagaa ggaatcggct tactggatgt
     5701 ttattttaaa gaagatagtg atctgggacc tcgttttatt tatgagctgt tagaaccact
     5761 cattcctaaa gcatttttca aagactctgt tctggctttt cttcttctgg aaaaagttgg
     5821 tgaacgacga ttccagaagc ttacccaatt ctttgaacct aaaaggcata cagcagaggg
     5881 atatcaaaaa atcacaaaaa aatttttaga tgtttatcgc ttcaatgaag cagtgtacgc
     5941 tgctaatcaa gaaaacatcg aatcgctgaa taaatctttt gaagaatctg aatcagtagc
     6001 attacttgaa gcagattcga cattcgattg gcagtttttg aatgattggc tggcaaaaaa
     6061 acatattgca agtcttgatg atccaatcat caaacaagtc gacatgtatc atcagcttta
     6121 cggtttcgat gaattgacac ttggcgaact gatcagtcaa tcttatgact ttagtgaaca
     6181 aaaagtatct gaaaaagaac tgcaaaaagc cattttggca atgcagagac gtccggtgaa
     6241 gcaaacaaaa gaaacagttg cggtttctga aaacagtaat gaaccctcgt caaacgaaca
     6301 tttgacaaac gcagcgcttc agctgataaa agaagcacag agcattccac cgatgaaata
     6361 tctggaagca atcaagtcag aaaaaggcgg atatgtttct aaacaagaaa actggttact
     6421 gcaagatctt gtttctcaat caggtctttc cagtagcgtg atcaatgttt tgctgaatta
     6481 tgtactagtg atcaagaata atgctagctt aaatgccagt tatgtaaata cagtggcaaa
     6541 tgaatgggca caaaagaaaa tcgccacagc agaagaagcg atccagcaca ttcggcaaat
     6601 aagtaatcaa gcaaaacaat caaaacaaaa gaagcaaaca aattatcaaa ctggcaagcg
     6661 aaatgttcga cgggaaaagc ttcctgattg ggtcaaccag ccaaaagatg aaaaacagat
     6721 cagcagcgag aaaaaagcag agattgaccg ccgttttaaa gaatacctgg caaaaaagga
     6781 gggtgatatc tagtggaaga cgtaggcaaa gaattgaaga ggatcatcag caaacgcaat
     6841 tatcaagaaa agtatgctga aatgatcgct gaagtcttaa atgatgcgga tgttcaagct
     6901 tttctaagcg cacaccaaga agaattgaca caagcggata ttgaaaaaag ttacgctaaa
     6961 ttgtatgaat tcgtgcaaga aaagcgtaaa tatcaaaaaa acgatccaac gatgatcgct
     7021 cccggctatg agccgaagct gacgttgaat tttcattatg tggatgtcac ttatgtacca
     7081 acggaagcat tgttagcacg gcaaagacaa gaagaaatca gaagccgagt aaaagcaatg
     7141 gatatgccaa aagatattca atcggcttct tttgaaaact ttgaacgtac agatggacga
     7201 ggatatgcga gcttagaagc cctagatttt gtagagaaat atactgctga tccaaaaggg
     7261 tatcataaag gactgtatct agtaggaagt tttggtattg gaaaaacata tttattagga
     7321 gcaacagcga gagaattagc gattgctgga ttcaccacga cgctagtcca ttttccgaca
     7381 tttgccgttg agatgaaaca ggcaatcgga aaagatcaag tggcagaaaa gttagatgca
     7441 gtgaaacgtt cacctatttt gatgatcgat gacattggtg cagatgcgat gagtagttgg
     7501 atacgagacg aagtattcgg tgttatcctt caatatcgga tgcaggaaca gttaccaacc
     7561 ttcttctcat cgaattttac aatggatgaa ttggaacagc atttgtccgt cactcaacgt
     7621 ggagatgaag agccgctaaa agcgaaacgg ataatggaac gcatacgtta tttgacaaaa
     7681 gaaatcgaaa tgactggtcg caaccgacgc aatggttgaa cagttgacaa gtccaactga
     7741 catttgacct agtagtaaaa gcaggtcagc gaatcattat atggacagaa atgaccggag
     7801 acaatcaccg caacaagtaa tgcagaaact taaacggagg attttttatg agggctattt
     7861 tttctagtat atctgtagtt ggtctatttt ttcttttagc aggctgcggt agcgcagata
     7921 gcaacacagg ttctgttgat tcgattgatg cgggtcaaac agtaaaaaca agcagtacga
     7981 aaacggagag acaaagcata tttgtcggaa cgattgaaag tattactcaa gtagaagcag
     8041 aagaagaaac gcttcagttg aaactcgtcg atgtcatggc caaagaagat ccagcgaata
     8101 tgggaacgtc attcagcaat gacggagtga tcttaaatgc tactgctgaa aaaataagcg
     8161 tggacataaa tgaattgaaa atcggtgaca aagtccaatt tacattagtt gagatgccga
     8221 tcatgacgat gtctatccca ccgcaagttc ctggagattc gattattgat gtttcattga
     8281 acaaataacc aataacattt tttcaaaaat aagaaaatgt tgttttatag aacgaaaagg
     8341 tatatttttt aaggagatat gggaattttt ttattcctag tctccttatt ttatttggaa
     8401 aatgctataa ttatctagaa ggagtgaagc tatgaaccat ttagatttat ctcagcgctt
     8461 agccgcagtg ggtgactttg tccctgaatc cgctcgttta gcagacattg gatcagatca
     8521 cgcttactta cccgttgcat tgatattaag aggaaaaatc gaatatgcag ttgctggtga
     8581 agtcgttcaa ggtccttatg aatcagcacg taagcaagtc aaaaaagatg gattagaaga
     8641 ccgtatcacc gtacgccttg caaatggtct agatgctatc cagccacaag atggtattac
     8701 agctgtcacg attgcgggta tgggaggttc actgatccga gatatcctag aagctggcaa
     8761 gttgaatcaa cgcttaagcg gacaagaacg tttgatcctt cagccaaaca tcggagagcg
     8821 tacattacga tcttggctgc aagaaaatca gtataaaatc caatcagaaa aaatacttga
     8881 agaaaataaa aaaatatatg aaatcattgt agccgaaaaa gaacaaaaac cagttactta
     8941 caatgaaatg gagctgatgt tcgggccatt tctgcttgaa gagaaaagtg agatcttcaa
     9001 atcaaaatgg acaagagagc ttcaacaaag aaaagctatc cgtgaacagc tcaaacaagc
     9061 agctaatcaa gaggcacgtc aaaaacaaat cgagcaggaa atccaaatga ttgaggaggt
     9121 tttaaagcca tgacaatcag cggacgcact ttcatccaaa agtttgaaag ctattgtcct
     9181 cagtggctag ctgaagaagg agacccagtc ggactgcata tcggaacatt ggataagcca
     9241 atccaacgag taatgatgac attagacgtt cgtccagaag tggtcgaaga agcaatcaag
     9301 aaaaaaatcg atttgctgat tgctaaacat cctccaatct ttcgtcctgt aaaacggctg
     9361 gttactgatc agccacaaga aaaaatgtat gcagatttgt tgaaacatga tattgccgtc
     9421 tatgcagcac acacgaacat ggatattatt tgggatggac tgaatgactg gttttgtgaa
     9481 cttctcggta ttgaagtgga gagttattta gtaaagactc atgagattca ttataaaaaa
     9541 ctggccgttt atgtacctgt ggatcatgca cagaaaatgc gtgaagtatt agctgcagct
     9601 ggtgctggta cacaaggtga ttataccggt acaagtttta cctcgattgg acacggtcga
     9661 tttactccgg aagctggtgc acaaccagcg attgggaaag tagggaagac agaacaagtt
     9721 caagaggcaa aagttgaggt tattttgccg gaaactatcg aaaaacaagt gattcaagcc
     9781 atgcggtcag ctcatcctta tgaggaacct gcttatgatc ttttcgctat agatgaacca
     9841 gtcgaaatgt ttgggttagg acgagtaggt gaattgccgc aagaaatttc tatagaggca
     9901 tttgtagaac aagtaaaaga agcgttccaa ctagatggac tgagaatagt ccagccaaaa
     9961 aatgcaaaat cctctgtgaa gcgcattgcg atttgcggtg gaagcggtga aaaattttat
    10021 ccgcaagcaa tcgcccaaag agcagatgtc tatatcacgg gagatatcta ttatcatacg
    10081 gctcatgata tgcaaagcgc tgggttgatt gcaattgatc ccggtcatta tattgaatcg
    10141 ttatgcaagc aacgatttat cgagaaattt gaatcttgga aacaagaaga aaattgggat
    10201 attaactttt tcgtttctga aacaaataca aacccatttc aatttaatta aaaggagaga
    10261 atactatgta cgaaaatctt ttgccacgtt ttttacgata tgtcaaaaca gaaacacgct
    10321 ccgatgcaac aagcaaaacg actccatcga ctcagacaca agttgctttt gcgcaagaat
    10381 tgaaaaaaga attagaagag ttaggcttag ctgatgtttt ctataatgaa gataacggat
    10441 tcgttatggg aacattacca agcaatgttg atcatgaagt gcgttccatt ggatttattt
    10501 cccacatgga tacagcagat tttaatgcag taggagtcaa tccacaaatc attgaaaact
    10561 atgatggcga gtctacgatc gtactagatg cggaagaacg ctatacattg aatacaaaag
    10621 attttccaaa tttgaaaaat tatgaaggcc acacattgat cacaacagat ggcagtacat
    10681 tactgggagc agatgacaaa tcagggattg cggaaatcat gactgcaatg gagatcttga
    10741 tcaataatcc ttctatccct cacggtacca tcaaagtggc ttttggtcct gatgaagaaa
    10801 tcggtgtcgg agcggataaa ttcgatgttg agcaattcga tgtagatttt gcttatacag
    10861 tcgatggtgg tcctttaggt gagttgcagt atgaaacgtt tagtgctgct caagcaaata
    10921 ttacgatcca agggaaaaat gtacatccag ggacggcaaa agatacaatg atcaatgctc
    10981 tacagttagc aatcgatttt cataatcagc ttccagccga tgaagttcca gaaaaaacgg
    11041 aaggatacga aggtttcttc catttgatgg cattggatgg gaatcctgaa gaagcttcta
    11101 tgagttatat cattcgcgat catgatcgtg aaaaattcga agcacgcaaa gcacaaatca
    11161 cacatatcca agaagagttg aatcagcgat ttgatcaaga acgaatcaaa gtagatatgt
    11221 atgatcaata ttacaacatg aaagaaatca ttgaaaaaga tatgagcatc gtggaattag
    11281 cgaaacaagc aatgatcgag cttggtatct ctccagtgat cgaaccagtt cgtggaggaa
    11341 cagatggttc aaaaatctct tacatgggta ttccgacgcc aaatctattt gccggaggag
    11401 aaaatatgca cggacgattc gaatttgttt ctttacaagt aatggaaaaa gcaacagacg
    11461 tgattgtaaa aattgcagaa ttgaacacaa agagttaaaa tcgaaaaaga acagcttatg
    11521 acaaggcgtt cgtcccaatg atggaatgtc atctgtctaa ggctaaaggg caggacaatg
    11581 gaattggttg tcccgccctc ttttttgaag agtaaatagg aggtaaagaa tggaaaagct
    11641 gctagttttt tataacaaaa gttcagggaa agatgagggt gagaaattag cacgctggtt
    11701 tcaagattat gtaaaagaaa agcagccaga attatatgta gaactagctg aaactggccc
    11761 atcgattgat gatgcggaat tagtcaagaa agcagaagcc atagaagctg atactttagt
    11821 aattataggt ggagacggca cgattcatca tatcgtccaa gcgttccaaa aaacattatc
    11881 tcaataccat gtcggattac ttccaggtgg aactgtcaac aaccttgcac gtgttcttga
    11941 gattcccctc aaaaaagaag aagctgctga cgtgatcctg gaaggcaatg tacggaaaat
    12001 agactatggt caagtcaatg atgaagtcat tatctctaca ctgacaatcg gaatcttagc
    12061 agatacagca gtttggatca gtcaagaaga aaaacagcgt tacggctcgt ggatctttat
    12121 caagaagttt tttaagttat tgctgaagaa gaaaaaatat catttggatt tagaaacaga
    12181 cgaggagcat tggaaaggaa aatcccaact gttgacagtc acgatgtcta actccgtggg
    12241 cggttttaca aattttgacg actcagcgac cctagatgat ggaaaatttc atgtaacgat
    12301 cgtaccttcc ttagatattt tccgatttat tttttatttg cctagaatca tgagaggcaa
    12361 attctatacg atccccggca ttacttattt cacagccaaa cgcatgaaaa tccaagcgca
    12421 ggaaaaatct gtgggcactc gaacggatgg agacacgaca gatgatcttc cagttgaatt
    12481 atctgttgta tcaaaaggat taacagttta ttcaccaaag aaagaagaaa aagcttagaa
    12541 atagtagtta acagaaagtt ggaagaagag taatggatgt attgttacac aatcttttgt
    12601 ggttttacct tttcattgtg aatttttact tattttgtct gatggggtat gataaacatc
    12661 aggctagaac tagcggctgg cgtgttcctg agaataattt gatatttgtc agcctgttag
    12721 gcggtggttt gggaggattg atctctcgaa aagttttcca tcataaaact cggaaaaaga
    12781 agtttactat aggattcgct gcaggtgtac ttgttgattt gattctcatt tattttttcc
    12841 attaaggagc aggagatgtc gtctaaagga acgaaaacta aaatcattct gaatctgctt
    12901 ttgctgattg tgatttttgc cattatttat tatttaatca atcaatcatt tgcagatatc
    12961 tttgccgaat tgctgtctac cagtgttgtt gttttggcag cggctgttat attagggaca
    13021 gtttaccaaa tcgctgaagg gcgctcaatc aaagaaattg ctgaaccttt cgcgccgaat
    13081 tttacaacga aagatgggtt cttgacctct agctatattg cgttttaccg aatcgtttcg
    13141 tttggtacag gaacgctgat ctcggagatt tatttttata acaaaaaagg aatgaagcta
    13201 tcccagggag caggggtgac agcactccat atgattatgt acaaactggc agtgatcact
    13261 tatgcattag ctggcttggt catccaattc tctctttttt attctaatgg tccatcaatg
    13321 atctggttta tcatcgctgg tatccttttg acgatcgtga ttgttgcctt tttattggcg
    13381 atgtccgtca gcttgaatgt tcaaatattt tttgtcagct tatcgaataa gttatttaaa
    13441 agtgagcgtt tgcggaaatg ggtggataca tgcaatcagc agatctactc tttaagggaa
    13501 acggtccaaa ccattatcca agacaggact gcgctattgc ggatttatct atggaatatg
    13561 ttaaaacttg tattttggta tgtacttcct tatttgattc tgattgaaaa ccatcctaaa
    13621 atagattttc tactgaccat ttcgtttatc agctttgcag tagtgctttc aggggttatt
    13681 cctacgcctg ctggtattgg ttcctttgaa tttgtctatt tgttattatt cagaccagtt
    13741 gttggaacag ttgacgcagt ttcttcgctt ttactgtatc gtttcagttc ctttatcttg
    13801 ccatttctct atggatttgt gtatgtgatt gctgaacggc gaagagtgat caaagaagaa
    13861 ataatagaag taaaaaaaga gaataaagaa aatcaagggt aggtctttta taagatctac
    13921 cctttttatc aaatcaaaga aaaacgttat ggataagata attttacgtt tgtttggctc
    13981 tttttttata tacatacaat caattttgtt tttttaagat ttatataagt cgagaaaaaa
    14041 agtcaaaaaa ccatgtgtat attttgacca aaattgacca aagcgatata atagtttttg
    14101 taaagatcaa agaaataagt gatcttacct ttaattagga gggtatacct gtatgaatat
    14161 tgagaaaatg actacgacgc ttcaagaagc gattgctgaa gctcaacaga ttgcggtcac
    14221 tcgaaaacat caagatatcg acattgccca tgtatggaaa atctttttac aacctaatca
    14281 ttttggtcga aatttctata cggatgccgg attggatgtc gaatcttttg aacatgaaat
    14341 tgatcgtcta ctggatgagt atccagttgt ttctggtgga aacgtccaat atggacaaaa
    14401 cctcagccag aatttctttc gattattgaa tgaagcagac caaatacgtg aaagcttcgg
    14461 cgacgagttt ctttcaacgg aagtcgttat tttagcgttg atgaaattaa aaaattatcc
    14521 gttaactgtt tatttgaata aaaacggatt atctgaaaaa gaattgcgta aaaacatcga
    14581 agagatgaga ggaggagaaa gagtgacctc acaaaaccaa gaagaacaat acaaagcatt
    14641 agaaaaatac ggcgttgatt tagtacagca agtcaaaagc ggaaaaatgg atccaatcat
    14701 cggtcgtgat gaagaaatcc gtgatgtcat ccgtattctt tcacgaaaaa caaaaaataa
    14761 tcctgttctg ataggagaac ccggtgtagg gaaaacggca atcgttgaag ggcttgctca
    14821 acgtatcgta cgcaaagatg tgcctgaaaa tttaaaagat aaaacgattt tttcattaga
    14881 tatgggtgca ttgatcgctg gagcaaaatt ccgtggtgaa tttgaagaaa gactaaaagc
    14941 agtcttgaaa gaagtcaaaa aaagtgatgg acgtatcctc ttgttcatcg atgaaatcca
    15001 caatatcgta ggggcaggaa aaacagaagg aagtatggat gccggaaatc tgttgaaacc
    15061 aatgcttgct cgcggtgaat tacatttgat cggtgccaca actttagatg agtaccgtca
    15121 atatatggaa aaagacaaag cgttggaacg tcgctttcaa aaggtattag tcaaagaacc
    15181 aacagtggaa gatacgatca gtattctgcg tggattaaaa gaacgttttg agatccacca
    15241 tggtgttaac atccatgaca acgcattagt agcagcagct actttatctg accgttatat
    15301 tactgatcgg ttcttaccgg acaaagcaat cgatttgatc gatgaagcca gtgcaacgat
    15361 tcgggtagaa atgaactcta tgccaacgga attagatcaa gttactagac gtttgatgca
    15421 gttggaaatc gaagaagctg cactgaaaaa agaatcagat gatgcaagta aaaaacgctt
    15481 gaaaaatttg caagaagaat tagctgaact tcgtgaagaa gcaaatgcga tgaagatgca
    15541 gtgggaaaca gaaaaagaag aagtcaattc tgtttcagca aaacgtgctg aaatcgataa
    15601 agcaaaacac gaattagaag atgcagaaaa taactatgat ttagaacgcg ctgctgtatt
    15661 gcgtcatggt acgatacctc aattagaaaa agaattgaag gaattagaag ctaaagcaaa
    15721 agacagcgag attaagatgg ttcaagagtc cgttaccgaa aatgagattg ctcaagtcgt
    15781 tggcagatta acgggtatcc ccgtaacgaa acttgtagaa ggtgaacgag aaaaactaat
    15841 taaattgaat gaaacacttc ataaacgagt gattggtcaa gatgaagcag ttgatgcggt
    15901 cagtgacgcg gtaattcgtt cacgggctgg attgcaagac ccaaatcgtc ctctaggttc
    15961 tttccttttc ctaggaccaa ctggtgtcgg aaaaaccgaa ttagcgaaag cgttagctga
    16021 gaacttgttt gactcagaag atcatatggt acggatcgat atgagtgaat atatggaaaa
    16081 acatgctgtg tctcgtctag tcggagctcc tccaggctat gtaggttacg aagaaggtgg
    16141 tcaattaacg gaagctgtca gacgaaatcc ttatacaatc gtcttgttgg atgaaatcga
    16201 aaaagcgcat cccgatgtct tcaatatctt gttacaagtc ctagatgacg gacgcttgac
    16261 tgattcaaaa gggcgagtag tagatttcaa aaatacggtt ctgatcatga ccagcaatat
    16321 cggttcacag ttactgctgg aaggcgtgac tgctgatgga acgattccag aagcagtggc
    16381 tgaacaagtc aacacgctat tacgtggaaa cttcaaacca gaattcttga accgaatcga
    16441 cgacacgatt ttatttacac cattgagcct agacaacgtg aaaggaatcg ttgacaaaat
    16501 ggtggcgcaa ttggcacaac gtctggaaca tcaagaaatc ctgctgacaa tcagtgatga
    16561 agccaaaacg tggattgctg aaaatgctta tgaaccagca tatggtgcaa gaccattgaa
    16621 acgatttatc actaaggaag tagaaacacc actagctaaa gaaatcgttg caggacatgt
    16681 tatgcctaaa tcaaaagtaa cgattacttt acttgatgga caacttcatt tcaaaacaga
    16741 agagctagaa gaaatcgtct aatcttccat tgagactata aaagaaaaaa ggggtctgac
    16801 acaactagtg tcagacctcc ttttttcaaa taaacagtgt tccagaagta gtttcttcga
    16861 aaaataagcc gaaatttctt gaagctgccc gcttctgtca caacctcttt tattaggtat
    16921 ttttcacttt ttctaattta taactttcaa agtaacgctc atctttttta ctttgagacc
    16981 atgcaaacaa tcccattaag gcagcaccta tcgtatttgt cagcaagtcg cccatcgtat
    17041 ccatcaatgc tgctcgtccg acaaacagtg tgccatcaga agcagcaaaa cgttgaagat
    17101 tcatgcctaa gaactggtcg cataagaatt cccagaactc ccaaaacacg ccgcaaagtc
    17161 cagcaaacgc aaagccgaat aagaggaaca accaaggact ggtttttgat atctcagcat
    17221 ctttcatcaa gtaaccaatc aatccatagc caagagctgt caggaccatt ggactcaccg
    17281 catgtaagat tttgtcccaa aaagagatga tactgatcaa atgcatccct gtgcctaaaa
    17341 agacagagat gaacaagaaa aaccagtaaa acaaaacgat tgctgctggt attttaagtt
    17401 tgaaaacttt caaaatgact tgtggcatga aaatcaatgc aatccctaat aaagattcca
    17461 cgataaatgt gattccttga gaagtcgaat atttttgtgt gataaattca taaaaattat
    17521 agcagacaac gaatacacca aaagcaagta aagctatttg cacctttttg atttctttaa
    17581 tcactgaaag tccctccatc taaaaatctt tttacttctg aaagcaactc aatttctgaa
    17641 gaaaccactt ttccgttcaa tttgatcaaa cctattgtat ataagttcag gtagtgaaat
    17701 tgattttcag ctgtttcttg tagtgcagcg atttttttcg ggtgggttcc gccttgctgt
    17761 cgagtatcag tatataagcc gatcactggg atgcctttcg cataagccac accgatttcc
    17821 gatgctaccc cggcatcgat cgtcagccca tctagtaaag caatcattaa atcactagcc
    17881 aacacttttt ctgtgtcagc aagagcaatc attcgactgt ctgcataagc agttttgtca
    17941 ttgattgctt cattttcttg aggaagatag atcgaaagct cagaagagag ctctctgatt
    18001 tctttaacta actgttggtt atataacaaa tcacttttag caaacattgg tgcggcaaaa
    18061 taaattttca tctaaacatc atccttttta tcaaaaataa tcgctaaagt aagcttccat
    18121 gtttggaatg atacgctcta gtgacttcag tgtttcttca tctgtttcta accgttcgat
    18181 tcttgccaaa taagaagcac ggcgataatt catcatcaag caagtcaatg tttggatatc
    18241 cagtcgaacg gattttccta taggttcatt taagacacgg acttgtccct gttcatccca
    18301 tgttaaagcg aagacaccat tattccaagg agcgacagga tcttcaatga tgaaatgaaa
    18361 agcatcagct gttccaacaa acggaaaccg ttgaaggaat tcttttacat caacgatccg
    18421 agccataaaa tatggttcga tctgctcttt gatctgacta tcttcaatca aaaatgctaa
    18481 aggctcattt ttataaatat ctccatgtac ccagtagatc atggaaaaat gtgcggaaat
    18541 aaagttccat aagccatttc gagcttcttg atcaagatag aacatttctt ttacgtgaaa
    18601 tatttcttca gctacccagt agaacagaac acctcttggt tcatggttgc tatcgtagta
    18661 gacagcggct gtgcgctcat cttcattttc gaaacgccaa tactcttccc aatgaaaatc
    18721 actgcggaat aaagcaccat ggttttgttg ggcaaatttg tcataaacgg aaaatacgtc
    18781 tggatgatca actggtaatc gctcaaccat accacttact gggatttgtt taggtagctg
    18841 tgtatctctg attttaaagg aaagtttatc agacatgatt tcccatcctt tacgccgata
    18901 gtaggggata ctgtaaggat acaaataaga gatccattgc ttattcttgc gcatattatc
    18961 caatgcaacg ataattagat cttgcatcaa tccatggtta gcatattctg gataagttcc
    19021 aactccagtc acaccaccca ttttataacg tgcaccgtga atattgactt cacaaggata
    19081 gatagcgatt tgagagatca gtttattttc gtggaaccat ccgaacactt tcgatacttc
    19141 caaaatcggt tttttggaac ggataaaagc tcgtttattc tcaaacccgc tttcctcgat
    19201 gtctgcttca gtcacttgga acacatagga aagcaattca ttgaattgat cgatatgttc
    19261 ttgagtgacc ggttttaatt caagattttc tctaaaatct ttttgatcca ttttttcatc
    19321 tccatttatt agcttgataa cagtatagca aaattctaca aaaaaaactt ctatagctga
    19381 ctaattaaaa actctttaaa ttactgcttt tcgttggtat aatggaaagt gctgattata
    19441 tgaaaagagg agacgctatg gatataaaga aaatgaaaga acgccaagag cagattcgta
    19501 atttttcgat catcgctcat atcgaccatg ggaaatctac attggccgat cgtattttag
    19561 aaatgacgca cactgtgacg tctcgcgaaa tgcaagatca gctgttagac tcgatggatt
    19621 tggaacgtga acgtggaatc acgattaaat taaatgctgt agaactcaat tacacagcga
    19681 aaaatggaga aacatatatc tttcacttga ttgacacccc agggcacgtc gacttcacct
    19741 atgaagtctc tcgaagcctt gctgcctgtg agggtgccgt tttagtggtc gatgcagcac
    19801 aaggaatcga agcacaaaca ttggccaatg tttatttagc gttagacaat gatttggaga
    19861 tcctgccagt catcaataaa atcgacctac ccgcagcaga tcccgaacgt gtccgtcaag
    19921 aaatcgaaga cgtaatcgga atcgatgcta gtgaagctgt attagccagt gccaaatcag
    19981 ggattgggat tgaagatatt ctcgagcaga tcgtggaata tgttcctgca ccaagtggag
    20041 acttagatgc accgctaaaa gctttgatct ttgactctat ttatgatagt taccgcggtg
    20101 ttgttctaaa tgtccggatc acggacggta tggtcaaacc cggagacaaa atcaaactga
    20161 tgagtaatgg caaaacattt gatgttacag aggtcggggt cttctcgcca aaagcagttg
    20221 ctagagactt tttgatggtc ggtgatgtcg gttatatcac agcaagtatc aaaactgtcc
    20281 aagatacacg agtaggggat acagtaacgt tagcagacaa tcctgccgct gaagctttac
    20341 caggttaccg aaaaatgaat ccgatggttt attgtggtct ttatccaatt gatacttctc
    20401 gttacaacga tttgcgggaa gctttagaaa aactgcaatt gaatgatgct gctttacaat
    20461 tcgaaccaga aacctctcaa gcattaggtt ttgggttccg ctgtggattc ctcggacttc
    20521 ttcatatgga tgtcgtccaa gaacgcttgg aacgggaatt caatctagaa ttgatcacga
    20581 ctgcaccatc cgttatttac catgttaata aaacggatgg tacgactgct acagtagata
    20641 acccggcaga ttttcctgaa cctgtaacga ttcaggatgt agaagaacca ttcgtcaaag
    20701 cacagatcat ggtcccaaat gattttgtcg gtgcagtaat ggaactttct caacgaaaac
    20761 ggggagaatt cattacgatg gattatttag atgattaccg agtaaatgtc gtctataata
    20821 ttccgctttc tgaaattgtc tttgactttt tcgacaaatt gaaatcaagc acgaaaggct
    20881 atgcctcatt ggattatgaa atgtctggtt atcaaaaaag caagttagtg aagatggaca
    20941 tcttgctgaa tggagaaaaa gtcgatgcat taagctttat cgttcaccga gactttgcct
    21001 atgaacgtgg aaaagcaatc gttgaaaaac tgaaaaaact aatcccacgc caacaatttg
    21061 aggtgccaat ccaagcagct attggacaaa aaatcgttgc ccgttcagac atcaaagcct
    21121 tgcgtaaaaa cgtcttggct aaatgttatg gtggagatgt ttctcgtaaa cgtaaactct
    21181 tagagaaaca aaaagaaggg aagaagcgga tgaaacaaat cggctccgtc gaagttccgc
    21241 aagaagcctt catggccgtt ctgaaaatgg acgaggacga accgaagaaa tagcaagggt
    21301 ttgtttgaaa acagtgtaaa aaataaacca tgtttacatg gatttgccac cggtttgcca
    21361 ccggtggttt ttttcggtgg caaaaatcaa gaattctttc taagattttc catgatatcg
    21421 accgtttcat ttctcatctt atctgttaca tgcgagtaag tatccatcgt aattgaaatt
    21481 ctgctgtggc ccaatcgttc agagatttct ttcatttttg cgccattttc gagaagaagt
    21541 gtagcatgag tatgtctgag agaatggaag ttaaaagaga gggagagtgc attcgatatc
    21601 ctcctagtat tccatttcac tacacttggc gtaactagct caccgtcttc cttcgtacat
    21661 actgcatttg aatcaatgta gagctttcca tacttcattc gattttctaa ttgttgtttc
    21721 ttatgttttt tcagaattgc tagcaaggtt tgtccaataa aaatcgttcg attggaactg
    21781 cttgtctttg gcgtaccata tacccatgcg ccgtcattct ttaccatttg tttctctaca
    21841 gtaattgttc catttgaaaa atcgacatca tcccacgtca gaccacaaac ttcgccaacg
    21901 cgcattcccg tataaaatcc aatattcaaa ggaatataga aaggatgtcc ttcaggagta
    21961 atttctagca tatgatcaaa gtcctcaaga gaaatgattt ttagatcttt tttagtcgtt
    22021 ggtcgtgctt catattttgg tatttttaca tacagcatag gattttgctt gattaatccc
    22081 caaggataaa ccgccatatt cagcgcattc ttaaggacag agtgagtaat agtcattgtt
    22141 ttcttcgagt aacccttttt aaattcagca ttgatgaaat tttgtaaaag agcaggggag
    22201 agatccgtaa gttttttctt tcctaaataa ccgtttatat gatttttgat ggtaaatcgg
    22261 tagttttcat aggtattgta ttttagattt agtttaacgt attcctccat ccaaaaatca
    22321 aggtattgtt ttactcgagt atccgtacct aaaaagtatt gtcctgtttc gtcaatatct
    22381 gataaaactt ttcgtaaagc agcttcggcc tctggtcggg tgtctccgcc aactttctcc
    22441 actttttttc ttgagccatc atcattgata tcttcgccgt ttgtataatt aagcgagaca
    22501 aataaaaaag ccatttatgt tacactc
//
LOCUS       DACEKH010000039        22213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEKH010000039 DACEKH010000000
VERSION     DACEKH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.040"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            171..515
                     /locus_tag="IW662_001407"
     CDS             171..515
                     /locus_tag="IW662_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ0866240.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="IW662_001408"
                     /pseudo
     CDS             516..935
                     /locus_tag="IW662_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="IW662_001409"
     CDS             complement(1027..1377)
                     /locus_tag="IW662_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866241.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="IW662_001410"
     CDS             complement(1343..2674)
                     /locus_tag="IW662_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ0866242.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="IW662_001411"
     CDS             2930..3496
                     /locus_tag="IW662_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0866243.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="IW662_001412"
     tRNA            3641..3728
                     /locus_tag="IW662_001412"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /locus_tag="IW662_001413"
     CDS             4062..5036
                     /locus_tag="IW662_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0866244.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5361..7256
                     /gene="asnB"
                     /locus_tag="IW662_001414"
     CDS             5361..7256
                     /gene="asnB"
                     /locus_tag="IW662_001414"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ0866245.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7451..7897)
                     /locus_tag="IW662_001415"
     CDS             complement(7451..7897)
                     /locus_tag="IW662_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0866246.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8125..8274
                     /gene="rpmG"
                     /locus_tag="IW662_001416"
     CDS             8125..8274
                     /gene="rpmG"
                     /locus_tag="IW662_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0866247.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8362..8880
                     /locus_tag="IW662_001417"
     CDS             8362..8880
                     /locus_tag="IW662_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0866248.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8957..9913)
                     /locus_tag="IW662_001418"
     CDS             complement(8957..9913)
                     /locus_tag="IW662_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0866249.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10079..10885
                     /locus_tag="IW662_001419"
     CDS             10079..10885
                     /locus_tag="IW662_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866250.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10882..11871
                     /locus_tag="IW662_001420"
     CDS             10882..11871
                     /locus_tag="IW662_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0866251.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11868..12872
                     /locus_tag="IW662_001421"
     CDS             11868..12872
                     /locus_tag="IW662_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0866252.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13004..13546
                     /locus_tag="IW662_001422"
     CDS             13004..13546
                     /locus_tag="IW662_001422"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ0866253.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13566..14264
                     /locus_tag="IW662_001423"
     CDS             13566..14264
                     /locus_tag="IW662_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0866254.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14285..14497
                     /locus_tag="IW662_001424"
     CDS             14285..14497
                     /locus_tag="IW662_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ0866255.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14497..15459
                     /locus_tag="IW662_001425"
     CDS             14497..15459
                     /locus_tag="IW662_001425"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ0866256.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15477..15872
                     /locus_tag="IW662_001426"
     CDS             15477..15872
                     /locus_tag="IW662_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0866257.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16035..16400
                     /locus_tag="IW662_001427"
     CDS             16035..16400
                     /locus_tag="IW662_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ0866258.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16397..18208
                     /locus_tag="IW662_001428"
     CDS             16397..18208
                     /locus_tag="IW662_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ0866259.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18270..18437)
                     /locus_tag="IW662_001429"
     CDS             complement(18270..18437)
                     /locus_tag="IW662_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ0866260.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18685..19674
                     /gene="galE"
                     /locus_tag="IW662_001430"
     CDS             18685..19674
                     /gene="galE"
                     /locus_tag="IW662_001430"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ0866261.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19686..21170
                     /gene="galT"
                     /locus_tag="IW662_001431"
     CDS             19686..21170
                     /gene="galT"
                     /locus_tag="IW662_001431"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ0866262.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21194..22174
                     /locus_tag="IW662_001432"
     CDS             21194..22174
                     /locus_tag="IW662_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0866263.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         7208 a         3804 c         4621 g         6580 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gacgggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agagagaaaa
     5281 tattcgtccg ctatttattc tatgataaga tgcatatgaa tgaaataaac agtaatttta
     5341 tattaggatg tgaaagttaa atgtgcggta ttgtaggttt tgttaacgac aaggacaaca
     5401 aaaaaacaat tattaataca atgatggatc gaatcgttca ccgtggacct aatagttcag
     5461 gagaatatat cgataaacat gttgctttag gattcagaag attaagtatt attgacttag
     5521 agggcggtac tcagcctatt tacaatgaag atcggacaaa gatcattatt tttaatggcg
     5581 aaatctataa ttaccagcct ttgagagaag aattgattgc agctggccac gtttttcaaa
     5641 cacatgcaga tacagaagtc cttttgcatg gttacgaaga atggggaaca gaattattgc
     5701 aaaagatccg cggaatgttt gcttttgcta tttgggataa tgaaaagaat gagttattcg
     5761 gtgcaagaga ccactttggg atcaaaccat attattatgc agaaatgaac ggaacattta
     5821 tgtttggttc agaaatcaaa agttttctgc ctcatcctga ttttaacaaa gaattgaaca
     5881 aagaagcgtt gaaaccttat atgactttcc aatattctcc attaaacggc gaaacattct
     5941 tcaaagatgt ctatcgtttg cctgaaggcc attattatac gtataaagat ggaaaaatag
     6001 acattcagca atattgggat gcagattttg aaacaaaaga aacgcattcc cgacaagaat
     6061 ggatcgaaaa aatcgacgaa accgttcaag cttctattga agcacatacg gtcagtgatg
     6121 tagaagttgg ttctttctta tccagcgggg ttgattccag ttatgtaacc tctgtattga
     6181 aaccagatca ttctttctca atcgggtttg atgataaaac ctataatgaa gcgattgaag
     6241 ccagaaaact aaccgaacta ttggacttag ataatacagc agcagtgatc gacggagaca
     6301 tgtcgttcaa agcattccct ttgatccagt atcacttaga tgaacctgat tcaaatcctt
     6361 cttgcgtacc tttgtacttt ttagcaaatc ttgcttcaca aagtgtgcgg gtagtccaat
     6421 ctggagaagg ggcggatgaa ctgtttgcgg gataccaaac gtatggattc catacgaatt
     6481 caaaattcat ccgtgtgatt gctcaaggat tgaaaaaatt accaaaagga acacgctata
     6541 atttaggacg taaaatcgga aaaatgaaaa atttccatgg acggatccat ttatatgaat
     6601 cacttgctcc tgcaaaagaa tattttatcg gtcatgctcg agtgttcgaa gaatcagaag
     6661 catcagaagt gttaacacca aaatatcaaa cagcaccatc tgtggatgaa atcatgactg
     6721 ttcattacga gaaaacagaa ggaatcaaag atgaagtcaa taagatgcaa tatgtcgatc
     6781 ttcatcaatg gatgccaaaa gatatcttgt taaaggcaga taaactttct atggcaagtt
     6841 ctttggaagt ccgagtacct ttattggata tcgaagtcat gaaattggct caacaaatac
     6901 caagtaagtt tttattgaat caaaacaaca caaaagatat tttcagacaa gctgcgaaca
     6961 aacatcttcc ggaaaaatgg tcgaatcgtg tgaaattagg attccctgtg ccaatcaaag
     7021 cttggttgaa agaagaacac ggctacgaac aagtcaaagc attgttcgaa gcagattttg
     7081 caaaagaatt ttttgatcaa gaaaaaatca tgaagttgct agatgaccat cacgaaggac
     7141 gtaaagaaga gcaaagaaaa atctggacaa tcttcagttt cttgacatgg tataaagtct
     7201 actttgtaga tgaaacgatt ccgcaagcag aagcaatcga ctatgtgact gtttgatgaa
     7261 aatcaaatat caataaaaaa gaggttttct cctaccgttt aacactgagt gataagaaac
     7321 aatttcaaaa aacagctctt cccgttttat agatattatg acttatggtt cggagttggc
     7381 agaggaacac cattgtttcg aacataaggg ggttgtgaca cgattttcgt cacaacccct
     7441 ttacatatgt tcaataatcg atttcttttt cttttgtccc atctttttta taaatgaaca
     7501 aagttgattc tttgttttgt gctacttgct ttcctttttc gattgcttct ttttttgtat
     7561 caaaatggtt gcttgccttt tttgcccctt tagatttcac gatccattgt tcttcctcat
     7621 attctacgat cacatctgaa tcaagcagtt ttccagcacg aggattcgta tcgtgttcat
     7681 ccgtcttgct tggatttttt tctttttcga aagttcgttt ttctgattca ctagcatctt
     7741 cataccattt ttttgcttga tcaatggcaa ttgggatcgc acggttatca gggtaccctt
     7801 catctaataa tgcatttgcg atgtcgattg ctttttttct tgtcaattta tctaagtttt
     7861 tcattgatgc aggaaagtcc ttcatgttcc atggcatatt agtatccccc tttattaaaa
     7921 aacataatct atataaacaa gtgtaagggt atttatacaa aaagaaaaag attttgtttg
     7981 agaagtaaaa ccgttgtatt tatcgtcaag tagttttact taacaaaatc gtgaaaaaca
     8041 ctagcaatct agaaaaaatc atggtaagat aatcaagtct gagagtcatc ttagaggatt
     8101 cttgtaaaaa tgggagggaa aaacatgcgc gtaaacatca ctttagagtg tacttcttgt
     8161 aaagaacgta actatctaac aagcaaaaac aaacgtaaca atcctgatcg tttggaaaaa
     8221 caaaaatatt gcccacgcga acgcaaagtt actttgcacc gtgaaacaaa ataatgtttt
     8281 taaagttctc aattccttgc tggtcaaaga attgagactt tttttatctc aaaacatcta
     8341 aaatcataag gagcggataa tatgtttatt tccatgtggg cacaagataa aaatggtctg
     8401 atcggcaaaa atggtttgtt accatggcga ttgccaaatg atatgcgttt ctttcgcgaa
     8461 catacaatgg atcgaatttt agtgatggga agaaagacct atgaaggaat gggggatctt
     8521 tcacttcctt atcgccatat catcgtgttg acaacgcagc caaattttcg cacgaaagaa
     8581 aatgcggaag taatgcattc gatcgatgaa cttttgtcgt atagtcaaac gatctcagaa
     8641 gatatctatg tttcgggtgg tagtcggatt tttcaagaac ttcttcctta cactggtcgg
     8701 atctggcgaa cattgatcga tagcacgttt gaaggcgata cgtatattgg gaatattgat
     8761 ttttcagact tcgcccttat agaggagtat gaggggataa cggatcaaga aaatctatat
     8821 gcccatcgtt ttcaaaaatg ggaaagagta aaaaataggg ttgcttcaaa agaagaataa
     8881 aatctatgcg tctagtttgt taataagaaa aaaagcatat cacgactaat cgtgaagatg
     8941 ctttttttag ttaaaatcaa tttagttttt ctttgatatc attgaccatt tcctgactgg
     9001 caataggtcc gttatatagc caactagatt caggtccgaa ttgcagtaca tgattgtctg
     9061 tcactgcttt aagattcttc cagcttggtt cattgaacat ggcagcatct ttgtcactgt
     9121 tcacaaggat gatgtagtca gcatctaatt cagctagctt ttcagaggaa acagcagacc
     9181 agtcagaagt ggcttttttg gagacctctt ccactagatc aggaacacca aattccagat
     9241 cgtcgtatac gattcttcca cttgaccgat ttttgctgac cataaaggct gagttgttgg
     9301 tcacccacaa cactgcagct gtcttgttct cgattttatc tgctagttct tctcgagttt
     9361 tcttgatgga tttttgataa tcggaaatga tcttttctgc ctctttttct tttgccaaag
     9421 ctttgccgac atcttttagt tgttcttccc atgtgacatc tgttccgttt tttacaacat
     9481 aagttggcgc gattttgcta tattgttcgt atttcccgcc ttcgacagta gcggaagaag
     9541 aaatcaacag caaatcaggc tcaaagctta acactttctc ataaggaagg tcataagaaa
     9601 tcgttgggac atcctttaaa tcatcttgca gataatcttg aatggaacca ctgccaacag
     9661 tccactgggc gataggtttt tcccctagtg caatcagata gtcttctaag taactgccga
     9721 tgatccgttt gggcgcgttt ggaatctcta ctttatgtcc aagtgtatct gtgactgtat
     9781 gagtactatt ggatgaagtc gtcgaattgg cagttgtagc tgtttcttta tctgaagagg
     9841 gggagcaagc gctcagtact ccagccagca gcaatatgct agataatgtg aatacttttg
     9901 tttttaattt catttctttc ctcctaacat aattgagaat ctttctcatt ggttattatt
     9961 cctgatattt cgaacgctgt caaccgcttc atttggctat agatatgaaa gtagcgcttg
    10021 acgaaatgtt gaaacagcgt cataattgag aatgattttc agcgaaagga tgggtgaaat
    10081 gacgagatta agagcagctg acattgcagc cggctacgaa aacaaaaaaa tcattgaaaa
    10141 catgtctgtt acgattcctg aagggaaaat cactagtttg attgggccaa atggaagtgg
    10201 aaaatctact ttattgaaaa gttttacccg tatactacct gtgaaatcag gaaagatcat
    10261 tgtcaataac tctgatatct ctacatacag ttcaaaagaa cttgcaaaaa aaattgcatt
    10321 attagcacaa actagtgaac accctacagg tatgacagta gaagaagtag tatcttacgg
    10381 acgataccct tatcaaaaac tgttcagtgg catggacacg caagatcatg aagcaatcga
    10441 gtgggcgttg gatgctacag gtctagctgg atttcgtatg cgggatatcg caacgctgtc
    10501 tggtggacaa acccagcgtg tctggatcgc tatggcgctt gctcaggaaa cagatatctt
    10561 gatcttggat gaaccgacaa catttttaga tccggcacac caattagaga tcctacagtt
    10621 attgactgaa atcaatcaaa tgaaacaaac aacgatcttg atgtcgattc atgacattaa
    10681 tcatgcttca cgtttttcag attatttatt agcaatgaaa aacggtaagt tgatcacaga
    10741 aggtacacct gaagaagtga tcacaaaaga gtgtatgaaa aaaatatttg agattgatgc
    10801 aacgattgtc acgcttccaa gaacaaataa accagttttt ctaacctatg atttgttgcc
    10861 aaaagagcag gaggatcaat tatgaggact atgaagaaaa atagactcct gcttttggtc
    10921 tgttttgttc ttttgattat tttttttgtt gctctttcaa atggcgcagc gactgtggga
    10981 aaagcagatt tttggcaggc gttgtttgat tttgatagat caaaacaaag tcaccaaatc
    11041 atacgaaata ttcgtttgcc tcgtgtggcg ggagcatttc ttgtagggag ttgttttgca
    11101 ttgagcggtg cattgatgca aggagtgaca agaaatcctt tagcagattc aggcttacta
    11161 ggaatcaacg cgggtgcttc gttggcaatg gcccttagtt ttgctttttg gcctaaagct
    11221 tctgattttt ctgtgtttct aatgtcattg gcaggttctt ttgctgtaac tttgctcgta
    11281 tttggttttt tgcagttttc gaaaatgggc atgaatcctt tacagctgat tttagctggc
    11341 gtagctatta gttctttttt tactgctatc agccaagcac ttacccaatt ctttaatctg
    11401 cagcaggatc ttgctttttg gtttgtaggc ggagctgcca atatcacatg gaaacaatta
    11461 gcagtattgt ttcctttata tatacttgct gttttggttt ctttttcatt aggaaaagca
    11521 atatctttga ttcatttgag tgaaacacat gcattagctc ttggaagtca tcctaaacga
    11581 acgcggttga ttgttttctg tctagttgca gtgttagcag ccattgccgt ttcgttagtc
    11641 ggacctgttt catttattgg gctgttggtt ccgccagttg ttcgcggaat ggttggaacg
    11701 gactatcgtt atgttctacc tgtctctttt ttagttggcg gtggattggt tatgttggcg
    11761 gattttacag cgaggatgat caatccccct tttgaaacac cttttggatt agtcattgca
    11821 ttgattggcg ttccgttttt actgatacaa gtaaggaggg aacaacggtg aaaaaaatat
    11881 attggacgtt ctatttcttg ctttttgtac ttgtcatcct gtctataggt agtttaatga
    11941 ttggtacgcc ttttatttac ctggatcagc tttttgccac gttcataggg aaagaatcat
    12001 cttctttggc gctgattgtt agagaatttc gagttcctcg attactgatc agtttattag
    12061 caggagcttg tcttggcgtt tctggttata tcttgcaagg agtaacaaga aatgaattgg
    12121 cagattctag tatattgggc attaatgccg gagcaggatt tttagtgatg ctttatttag
    12181 gtttcttttc gcactattcg ctgccgtttc tattgctagt tgtagccttt ctaggtggtg
    12241 ttttagctgc ttgctgtgtc tatcttgcgg cctatgatcg aaatggcttt ttgggtatga
    12301 atcgaatctt attgtccggt attgccgtta atgccggcct ttctgcatta acattgctat
    12361 gcacgatcca attatctaag gaaaattatg gatttgtgaa tgcatggtta gcgggatcaa
    12421 tctggggtgc gagctggcct tatgtttggg cattgcttcc atgggcggtt atcttaattc
    12481 cgctaggagg actttatgca cagaggattg tattgctatc atttggtcaa gaacgcgcgc
    12541 aaggattagg ccttaacgtg gctaaaagtc aaaaaatcct tttgttgtta gctgttgctt
    12601 tggcaagcgg tagtgtagcg gtgacaggta gtttatcttt tgtaggacta ctagctcctc
    12661 atgctgcgaa gctagtcgta cgtaaagaaa ataactggac ctttgttctg agcggtttgt
    12721 tcggttccat ttttctgctt agtgcagata tggttgctag agtgattcta ctaagtgggg
    12781 aagtacctac aggaattttg attgcccttt ttggtgcgcc atattttctg tatgcacttt
    12841 ttatcagaca aaaacacgcg ctgaacagtt gaaattcatt aaaaaaggaa ggccaatcat
    12901 ttctaggtct tctttttatt ctatttatgc atttatttga aagttcgatt gctatttttc
    12961 tttttttcct ataccatttt atgagagaag gaagggtgag tttttggacc aattgaaagt
    13021 aagattacgc cggaaaggaa tcgaaaagct gaaaaatctg gccaaaagtg gcaagaaaaa
    13081 ggaaaaagaa cagcgaatct ataaacagct attcgccagc gagtactgga agtcagcaaa
    13141 agtgataggg acgactttat cgaatgaatt tgaactggat acacagccgg ttatccaacg
    13201 agcagaaaaa gaacaaaaga aaatagttgt acctaaaact ttgccgaaac gtcagatggc
    13261 gttttatgaa atggatgaac agtccgttct tgtacgttcg agttttggtg ttcttgagcc
    13321 acaatcagag aagctctatt tgccggagat gatcgatctc ctcattgtac caggtattat
    13381 ctacaataga gccggttatc gaatcggttt tggcggtggt tattacgacc ggtatttaaa
    13441 taattttcat aaaaaaacat gtagccttgt ttttcgtgaa caattgaatg aacaatggca
    13501 accggagagc tttgatcaga agatccaaca attgtttatt gactaaaatg aaatgggagg
    13561 ttttcatgaa ttatcaacag caaataaaat tgcgtgcatg gttaagacga ccgttatgga
    13621 cctatgcttt tttagggata cagacagcgg tcttcctcat gatggaatta tttccgcgac
    13681 ttgagatccc ttattatgcc ggaatgtatg gtccttatct tgttcacttc aatgaatggt
    13741 ggcgtttagt gacaccaatc ttcatccatt ttggactgat gcatttcgta atgaactcac
    13801 tgatactgta ttttatgggg cagcaaatcg aggcaatcta tggccattgg cgttttttct
    13861 tgatttatat gttcagcggg ataatgggaa atacagcaag ctttgctttt aacgaagcga
    13921 atgttctgtc tggtggtgca agtacttcga tattcggttt gttcggtgcg ttgtttatct
    13981 tagggtttca ttttaaacat aacccagcga tccagcagct agtcagacat tttcttttat
    14041 ttattgtgtt gacatttgtt tttggtcttc tggatacctc tgtagatgtt tggggacatg
    14101 tcggtgggct aattggcgga ttagtgttag ggaatgtttt gggtctaccg aaacaacaga
    14161 caagctattc gatacaccaa aggattcttt ctacactagt ttttgtattt ctttttgtaa
    14221 tatgtatttt actgggttta aaaaaatatg gtttacttgt ataatatgaa ggaagtgctt
    14281 aaagatggag agcttatatg atgtacagca gctgttcaaa aaatttggca tctttatcta
    14341 tgtgggtgca cgcatctatg acattgaatt aatgactatc gaattgcgta atctgtatga
    14401 tagccgtttg attgatcatg atacatacat gacagcgtgg cgcatattaa aaagagagca
    14461 ccgaattgaa gaaagccgaa agaaaggaaa tttgtaatgg acaagaagat tattgggatt
    14521 gatttaggtg gaacaacagc aaaatttgca attttaacac cagaaggaga gattcagcaa
    14581 aaatggagca tcgataccaa tattttggat gatggtaagc atatcgttcc tgaaatcatt
    14641 gaatcaatca atcatcgttt gaatttatat aatatgaaag ccgaagactt tatcggaatc
    14701 ggcatgggta ccccaggaag cgtggacagc gaagctggaa cggttattgg tgcgtataac
    14761 ttaaactgga cagaagttca atttgtgaaa aaactgatcg aggcaggaac aggaatcaaa
    14821 ttcgctatcg ataatgacgc gaatgttgct gctttaggtg aacgatggaa aggtgctgga
    14881 gaaaacgatc cagacgttgt attcgttact ctaggaacag gtgttggcgg aggtatcgtt
    14941 gctggcggaa atttgattca cggcgtgagc ggagctggag gagaaatcgg acatatcact
    15001 gtggatcctg aagggttcga atgtacttgc ggtaaacgcg gatgtttaga aactgtatct
    15061 agcgctacag gtgtggtacg cctaggtcgt ttcttggcag aagaatacgc aggggattca
    15121 aaactgaaag caatgctaga caacggagaa gaagtaacaa gtaaagatat ctttgaaatg
    15181 gctcaagaag acgatccgtt tgcattgatg gttgttgatc gcgtatgctt ctacttaggt
    15241 ttagcttgcg gaaacttggg gaatacatta aatccgtcaa gtatcgtgtt aggtggagga
    15301 gtatcggcag caggagaatt tttacgtagc cgtgtagaga aatattttaa agaatttact
    15361 ttcccgcaag taagagaatc aacaaaaatc aaattagcag agcttggtaa tgaagctggt
    15421 gtcattggtg ctgcctcatt agccttgaaa tttgcttaaa aaaacggagg cattacatgg
    15481 ttttatgggt aattaatatc attttgctat tgattgtgct tgcaatcatc ctttgggaag
    15541 tctatctacg agtaatggcc aaacgttcag caacgacttt gacagaagaa gaatttcgag
    15601 aaggaatgag aaaagcacaa gtaatcgatg ttcgcgaaaa agatgtgttt gatgctgggc
    15661 atattttagg tgcaagaaac atcccttaca ctgttttgaa agattcatta ggatctattc
    15721 gaaaagacca gcctgtctat atttacgatc aaaagaaaag tttgagcatc cgtacagcaa
    15781 ataaattacg taaagcaggt tatcaagaga tttatttact taagggtgga tatgacggat
    15841 ggtctggaaa aatcaaaagt aaaaaagtat aaaacagatg attgtatgac agctttaaaa
    15901 accgaatcaa tcattagatt ttgaccaaga tcacatgaac ggcgcaatgg atagttgtgc
    15961 cgttcatttt ttttattttg tgctattatc tacatatata agctaagcga aaaggtagaa
    16021 agaaggaaac aactatgatc aaattaaaac gtgtttacct tgatgctgaa aaagaggatg
    16081 gctatagaat actcgtggat agattctggc cacgaggaat ttcaaaagaa aaagcgcagt
    16141 tagatctgtg gttgaaagaa attgcaccta gtaatgaagc acgcaaaact tttgaccatg
    16201 taccagaacg atttgcttca tttgcaaaga aataccagca agaattgatt ttcgaagaag
    16261 aaaagaagca agctattttt aaactcagtc aacttatgaa agaacatcca gtcattactt
    16321 ttgtttatgg tgcaaaaaat gaacatcaga accatgctgt tttattgaaa aaatttatcg
    16381 aaatggaggc atgcaaatga aaatagggat cattggtgcc ggacctagag gattaagtgt
    16441 agcagaaagg attctccata ataatcagga cagacattct cttgaacttt ttcttttcga
    16501 tcccaatggt cctggaggtc gagtttggcg gttgaatcag ccaccggagt tattaatgaa
    16561 ttctgtttct caacaagtga cgctttttac agatgaaaca ttgtccagtg gaggattggt
    16621 aacacaggga cccaacttgt ttcaatggag taaaggacct gctacagatt atatcgaaca
    16681 gacggtaacc aaaaataagc aactaattat agaagaaatc aatcgtctgg aaaaagatca
    16741 gcaatctact cgatgtttat acggactcta tcaacgctgg tttttctcaa agttgcaaga
    16801 agaatttcca gcttctattc agtttgttta ttctttagta aaaaaagtaa gcaaaagaaa
    16861 agatggttat cttttacaga cagatcagca aagctattct atcgatcagc ttgttttagc
    16921 aagcggtcac tgggaaaatg atgagacaga agaagaaaga gtgtgggtag actacgcaaa
    16981 gaaaaatgtt ttactgtatc agcttccttc taatcctgca gatgtagatc tttcgaagat
    17041 accgcctaaa gaaaatgtct ttttaagagg tctaggctta agtttttttg attatgtcgg
    17101 attgtttaca gtatcaagag gagggcgctt cgagaacgaa aagggaaaac tggtctatca
    17161 tccatcaggc aaggaaccga ttgtatacag tggatcaaga agggggctcc cttattaccc
    17221 tcgaggaaga aatcaaaagc aaggcggagc aatggcatgg ccacggttac taacaaatga
    17281 aaacctggag caatggcatc agtcgggatt tttgactggc gaaacatttt ttgactatct
    17341 gaaaaaagac gttgagttgt tttattacaa gaaaatcatc gaagaatatc acttgccgat
    17401 tccaaagaaa aattttgagc atgattttct ctccctctct tcagaggagt gtctcggaaa
    17461 gcatccagca ctaatccctt atagatggga ttggtcgtat ttagaaactc ctcttgccta
    17521 tcagaatgag cccttttcag aagctagccg aatatttatt gaagggcaga taaaagaagc
    17581 ggaaaaaggc aattgcacag gagcatttgc ttcaacattt gatgcattaa aagactggcg
    17641 tgatccaatc cggcaggcta ttgaatggca cgttttttct gctaaggaat acaaagaaat
    17701 actatgggga tggtttactc atctaaatag ctttttaacg atcggtccgc caatcattcg
    17761 gacgaaagaa ttagcagcac tgatagatgc gggtattttt catttgatcg aaccgccatt
    17821 tgatattcag caaaagcaca accgctatgt tatttctcta caagataaag aaatcagcag
    17881 tcgctatttg attgaagctc gattgccaca aacaagcttt tctcgttcaa gtaatcctgt
    17941 cataaaaaat ttgaaggaaa acaaacttgt tcgccctttt tcattttcag ataatacagg
    18001 aagttatcat tcagaagcta tagatgttga gcgaggtacg aatcgcgtga ttgatgcttc
    18061 aggggaaatc cagtctgact tttattgcta tggaatacct gttgaaggtg tggattggct
    18121 aactgcagca gtagctagac cttatacaga tccatggaat ctgagacagg cagataaaat
    18181 agcacaactt atattgactg acaaataaaa atacgcatga tgacttatct gttttttaga
    18241 aagtcttcat gcgtattttt aaataattat taacgagaaa cgcgtttacg cattgctttt
    18301 ttacggtttt cttcgatcat tgcttctttt tcgtcaattg gatcgatcac tgtttttttc
    18361 acagaagcaa ccaaaccagc aacggcagcg gctgttgcca gacttcctac taatattcca
    18421 gatacaaatt ttttcattga atccacctca tttattattt gttatatcta ttatggaaca
    18481 aaagagaaaa aaaggctagt tttacgtttg tttgtaaacc tttgcaaaaa atccttgaaa
    18541 atgtttacta aatgttttat tataaattta ccatatgaat ttacgaaatt ttactttgtt
    18601 tgttttcacc ataaaagtaa acttcgtttt ttaaattata tagaatcaac aaacacaatt
    18661 ttactaatca aaggagtgtt ttttatgaca attttagtac taggcggagc cggttatatc
    18721 ggttcgcacg ctgtagacca gctagttcaa aaggggtatc aagtagctgt ggtagataat
    18781 ttgctcacag gtcacaaaca agcagtacat ccagatgctc atttttatga aggagacatc
    18841 cgggataaag aatttctacg ttccgtattt gaaaaggaac cgattgaagg agtaatccat
    18901 tttgctgcaa gctctttagt aggagaatct gtcgaaaaac cattgatgta tttcaacaac
    18961 aacgtatacg gtatgcaaat cttacttgaa gtcatgcacg agttcaacgt gaataaaatc
    19021 gtattctctt ctactgcagc aacttatgga gagccgaaag agtctccaat cacagaagat
    19081 acaccagcta atcctaaaaa tccgtatggt gaaagtaaat tgatgatgga aaaaatgatg
    19141 aagtggtgtg accaagcata tggcatgcgc tatgtcgctt tgcgctactt caatgtagcc
    19201 ggagcaaaag cagatgcatc tatcggagaa gatcatacac cagaaacgca tcttgtccca
    19261 atcattttac aagtagctct tggtcaaaga aaagccttgg ctgtttatgg agacgattat
    19321 gatacaccag atggaacatg tatcagagat tatgttcaag tagaagattt gattgctgca
    19381 catattttag ctttggaata cttgaaagaa ggaaatgaaa gcaacttctt caatttaggc
    19441 agcagcaaag gatactcagt caaggaaatg ctagaagcag ctagagaagt gacaggaaaa
    19501 gaaattccag cagaaatagc accaagaaga gctggagatc ctagccgatt agtcgcttca
    19561 agtgaaaaag cgagagagat tctgggctgg aaacctgaat acacagatat caaagcgatt
    19621 atcaaaacag catgggattg gcatgtcagc catccaaatg gttatgaaga ataggggaat
    19681 gagatatgtc aatcagtcaa acgatttgtg attttgccac acttgcgatc gaagctggcg
    19741 gatggatgga aatggaccgc ttatatttac agaatcgtct tttagggatg gtaggagaag
    19801 atgctctaga aagcattgaa gtaagaccag ttaaggaaga atctgtagac ttattggatc
    19861 agcttgtcgc taaggcaaga gaaaatggtg ttctttcaga attgccagca gaacaagaga
    19921 tttttgaagc gcaattaatg gattttctca caccgccacc ttcagtcgtc aatgcattct
    19981 ttgcacagca ctattcgaaa agcccagagg aagcaactga gtatttcttt aatttaagta
    20041 agaaaaatga ctacatcaaa acaagagcca tcgcaaaaaa catcaaattt ccggcagagt
    20101 cggagtatgg tgaactggaa atcacaatca atttatctaa gccagaaaaa gatccaaaac
    20161 aaatcgcagc agaacgcgag gcggtaaaag tagactatcc tacctgcatg ctttgcatgg
    20221 aaaacgaagg atatagaggt cgcttgaact atccagcccg caccaatcac cggattattc
    20281 gaatgaatct agacggtgaa agctggggat tccaatattc cccttatgct tattacaatg
    20341 aacattcgat cattttatca gaacaacacc gcccgatgaa aatcacaaag gaaacatttg
    20401 ctagactgct tcggattact gaagtcttgc ctcattactt tgttggttcg aatgctgact
    20461 taccaattgt tggaggatcg attctttctc atgatcatta tcaggcagga cgtcatgaat
    20521 ttcctatggc aaaagcaccg atagaaaaac ttatcaaact agaagattat ccgcaagtca
    20581 tcgcaggagt cgtgaaatgg ccgatgtctg tgatccgttt gcaatcaaca gagaaaaact
    20641 tgcttattga agcagctaac tcagtattgg aaaaatggaa agcatattcg gatgaaacag
    20701 tatctatcag agctttttca gcagatggta caccacacca tacgattacg ccaattgcac
    20761 gaagaaggga caattacttt gagttggacc ttgtccttcg agacaacaat gtttcagaag
    20821 aacatcctga tgggattttt catccacata aagatgtcca acatatcaaa aaggagaaca
    20881 ttgggttgat cgaagtaatg gggcttgctg ttttaccacc tcgtttgaaa ccagaactag
    20941 tagaagtaga gcatttctta ttaggagaag aaaaccagat ggctgaatat catcgtccgt
    21001 gggcagaaca attaaaaaaa gaacatcctg atttgacgaa agatacggtc accgatgtcg
    21061 tacaaaaatc tgtaggtcag gtttttgcac gtgtactgga agacgctgga gtattcaagc
    21121 gagatgaaaa aggacaggca gcgtttgaac gatttactgc gattttataa acgtaaaaaa
    21181 ggaggagaga caaatggcaa caattaaaga cattgcgcag ttggcaggag tttctcctgc
    21241 tactgtttca agagttctca actatgatcc tgatctttcc gttggcttag ccacaaagca
    21301 aaaaattttt gaagcagcag aacgcttaaa ttatacgaaa catcgtaaaa atgcagcaaa
    21361 tactaagcaa acgcttcgcc tgatccagtg gtacgacgat acggaagagc ttgaagatct
    21421 ttattattta tctattcgcc taggaatcga aaaaaaggca gaagaagcaa acgttacttt
    21481 attgaaagaa acgtgggatt ccattagtga tcttccggca gatggaacga tcgcactagg
    21541 aaaattcgac gaagctcagt tattagaaat gaaaggatcc caaaatgcct tgcttttggt
    21601 agattctgac ggcacttctc aaggaatcga ctctctagtt gtcgacttta agtcaagtgt
    21661 tcaaaaagtc attgaacact ttgtctccca taaagcgcaa catatcgcta tgcttgctgg
    21721 aacagaatat acgaagaaaa accatcagcg gctaaaagat cccagaatta tggcatttca
    21781 acaagttttg gcagaaaaag gctacgacga aggtccaatt atcgaagcag atttttctgt
    21841 cgagtcagga tatcaagcag tgaagcagta tttaaaaacg aatccgcatg ttcctgatgc
    21901 gctttttgca gctaatgatg cattagcgat tggcgcactg aaagcgattc aagaaaaagg
    21961 tttagtggta ccagaagata tttctgtgat gggcttcaat gatgtcagtg tagcgaaata
    22021 tgtaacgccc tcgctttcta ctgtaaaagt ttataccgaa tggatgggag agctagcagt
    22081 ggagacgatt cttcaattaa gtaattccaa agcaccggtt tcaagaaaga ttattgtgga
    22141 aacagagttg attattcgag aatcaacgaa ataaaagatg gaaaagctgt gggaaaatgg
    22201 agtgataccg cag
//
LOCUS       DACEKH010000040        21837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEKH010000040 DACEKH010000000
VERSION     DACEKH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.041"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(236..436)
                     /locus_tag="IW662_001433"
     CDS             complement(236..436)
                     /locus_tag="IW662_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0866264.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(741..1973)
                     /locus_tag="IW662_001434"
     CDS             complement(741..1973)
                     /locus_tag="IW662_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0866265.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2227..2796
                     /locus_tag="IW662_001435"
     CDS             2227..2796
                     /locus_tag="IW662_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0866266.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2974..3414)
                     /locus_tag="IW662_001436"
     CDS             complement(2974..3414)
                     /locus_tag="IW662_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ0866267.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3572..4336
                     /gene="map"
                     /locus_tag="IW662_001437"
     CDS             3572..4336
                     /gene="map"
                     /locus_tag="IW662_001437"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ0866268.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4368..5291
                     /locus_tag="IW662_001438"
     CDS             4368..5291
                     /locus_tag="IW662_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ0866269.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5367..6506
                     /locus_tag="IW662_001439"
     CDS             5367..6506
                     /locus_tag="IW662_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ0866270.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6499..7299
                     /locus_tag="IW662_001440"
     CDS             6499..7299
                     /locus_tag="IW662_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0866271.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7299..8126
                     /locus_tag="IW662_001441"
     CDS             7299..8126
                     /locus_tag="IW662_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0866272.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8104..8838
                     /locus_tag="IW662_001442"
     CDS             8104..8838
                     /locus_tag="IW662_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0866273.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8938..9804
                     /gene="rfbA"
                     /locus_tag="IW662_001443"
     CDS             8938..9804
                     /gene="rfbA"
                     /locus_tag="IW662_001443"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ0866274.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9818..10390
                     /gene="rfbC"
                     /locus_tag="IW662_001444"
     CDS             9818..10390
                     /gene="rfbC"
                     /locus_tag="IW662_001444"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ0866275.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10412..11440
                     /gene="rfbB"
                     /locus_tag="IW662_001445"
     CDS             10412..11440
                     /gene="rfbB"
                     /locus_tag="IW662_001445"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ0866276.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11538..12389
                     /gene="rfbD"
                     /locus_tag="IW662_001446"
     CDS             11538..12389
                     /gene="rfbD"
                     /locus_tag="IW662_001446"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ0866277.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12424..14457
                     /locus_tag="IW662_001447"
     CDS             12424..14457
                     /locus_tag="IW662_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866278.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14501..15781
                     /locus_tag="IW662_001448"
     CDS             14501..15781
                     /locus_tag="IW662_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0866279.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15991..16797
                     /locus_tag="IW662_001449"
     CDS             15991..16797
                     /locus_tag="IW662_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0866280.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16809..18029
                     /locus_tag="IW662_001450"
     CDS             16809..18029
                     /locus_tag="IW662_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866281.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18019..19605
                     /locus_tag="IW662_001451"
     CDS             18019..19605
                     /locus_tag="IW662_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0866282.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19644..21782
                     /locus_tag="IW662_001452"
     CDS             19644..21782
                     /locus_tag="IW662_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0866283.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6888 a         3696 c         4543 g         6710 t
ORIGIN      
        1 aaaaagagta tacgaacgaa tgttcttttt gtgaaggcgg aatttaatat actgaataat
       61 agaaaacaag tacttgtgcc aaattatgtg ccaaaaaaat tcgaattcaa caaaatgtaa
      121 acaaatagaa aaccttctat attaagattt cctgcaataa caaacacaac agaagacgtg
      181 taatagttag tcaggaacgt acaaataacc cctgtatcct ttgcggtaca gggggttatt
      241 ttgtataagt agacattgta gtttctaaac tgctatgtcc taaacgtttc gatacgctaa
      301 gtatatttac tccttgataa agtaaaatag atgcatgcgt atgtcttagt ccatcaataa
      361 acagtcgaag agaaaatagg gaaaaattga ttactaaaga aaaacttact aggtcgacgg
      421 tgtagttgat tttcatactt caatagattc gaattaaacg atttcgattg gcttatacca
      481 gtcctatcca acgaaagata tatcgttctt aactctagcg gtataacttg aagctttacg
      541 taaagccaat gggaatgaac tggagatgac tttttattat ttttagtaat agctgatggg
      601 ttaatactta caactttcct tttcaaagta ccatataaaa aaacggtggg gttgtttagc
      661 gaaataaagg atctaagcca atagcttaga tcctttattc tatgctttat attatttttt
      721 gcatgtgtgc gaaagtatac tcaagcccaa tcgccatttc ggaaaatcgg tactgtagag
      781 ccatcttcac ggattccgtc gatatccatc ttgtcagatc ctaccatgaa atctacgtgg
      841 gtttgacttc tattcaatcc agcttctgct aattcttcat cggacatttc tgtaccgcct
      901 ttcacactga aggcataagc agaacctagt gctaagtgat tcgatgcgtt ttcatcaaat
      961 aatgtattga aaaagatgat gccagattga gaaataggtg aagggtcagg tacaagtgcg
     1021 acttctccta aacgacgcgc accttcatct gtatcaagca gttttgctaa gacatcttct
     1081 ccttgttcgg cagaaaaatc aacaactttt ccatctttaa aagtaaattt catacccgag
     1141 atgatggtgc cagcataact cagtggcttt gtacttgaaa tgtaaccatc tactcgatga
     1201 ctgtcaggtg ctgtgaacac ttcttccgtc ggcatatttg ccatgaattt ttctccacga
     1261 gcattatagc ttccagcgcc ttcccaaaga tggtttttag gcaatccgat aatgatatct
     1321 gtaccgggag cagtatagtg aagggcagaa aattgttctc gattcaattc ttctgctttt
     1381 tttgctaatt tttcatcatg cttcttccat gctaagacag ggtcttcttc atagacacga
     1441 gtcgttttga aaatctgatc ccataatgcc tcaacttgtt tttcttctgg cagttctggg
     1501 aagacttttg ctgcccattg tttaccagcc gcagccacga cagtccaact gactttattt
     1561 gcttgagttg ccttgcgtaa attcattagt gctttgccat tcgctgattg ataagacgct
     1621 actcgatggc tatctacacc agcaaatgca tcgggatcgg cagaaacaac gctgatcctg
     1681 cttgctcctt tttcaagcca ttcatccgct tgatcgattt tagattgtgg tacattctca
     1741 atacgatccg ttgcagcatg taaaagaaat tctctttgga tctggtcgtc agtccattga
     1801 acgataacct cagcggctcc tagtttataa gcttcttgtg tgatcaatcg agcaaggggt
     1861 gcctgttcca cgctgatctg taaaacaact gtgtgtcctt tttctgtggc tacgccagtt
     1921 tctgcaatta agcgtgcata tttttttaga agttcattaa aatcagataa cataataaaa
     1981 attcctcctc accatttttt ctggtaacta ttagcatgat accatttaat tatgattcaa
     2041 acaatgaggc tttaaataaa aaaaggaggt tattttttta aggttaagtt tcatgatata
     2101 tgttactact tgctatttca gcttgtgaag aaaatcaaaa actaattttg catatcaaaa
     2161 aaacatgtta caatgaaatt aatcaatggt ggaagcgtta taaaaaaatt aaggagatta
     2221 aaaaatatgg caagaaaaaa gacgattact agagaacaga ttttggcagc tgcttatgaa
     2281 gtagctgcca ccgatggatt tacacgattt actgcgagaa acatcgcgaa caaaatgaat
     2341 tgttccacgc aaccgattta tcttgagttt aaaaatatgg atgacttgag acaagctctt
     2401 tttgagcaga tctatgatca tttacaatat gaagtgttca ctgttgagca tacagggaat
     2461 acgattatcg atcttgcttt taattatatc cattttgcaa gaagagagaa gaagttgtat
     2521 cgttcactgt acttagaaga atatggtgga ggaaaagaga tgcagaattt ctcttatact
     2581 tactttgtga atacagtgaa acgcgatcca gcttatgtga atctttcaga agagcagatc
     2641 gaatctttgt ataatgggac atggatcata gctactggga tcgcagcttt gatgtcttca
     2701 agcatcatcc atccaactga tgaacagatt gaaaagatga tccaagacag tatagatgct
     2761 atcttgaaat tcaaagaacc aatcgaaatc gattgaatat actcaaaagt tccacaaatc
     2821 agtaggtaac aagagatgtg tcaaaataag gaaaagttcg tttttcttac agcgtatgca
     2881 tttttaactc ccgaagaact cttccatgaa tgcaatgaat aaaagagcca tacagattga
     2941 ataaatctgg atggctcttt tattgcaagt atattaaatt tttgcgacta gttctttagc
     3001 gaaagcttct aaattttcga tatcttcttc ttcagcattc agatccactt taacattttc
     3061 ggctcctttt gatgcgcctg tttttgcaaa agcagcatcg aaatcatcca ccgatttgca
     3121 gaattcatcg taaaaagtat caccagatcc gcagacaccg taaatttttc ccaatagatc
     3181 caattcttgc aagtcttcat aaaagtctac gatctcatca ggcagatcgc catcatcgta
     3241 tgtataagta gctactacgc aaatatctgc ctcctcgaat tcatctgcat ctacttgggt
     3301 acattcgttg atttcgactt cgatatccag attttcaaat gcttctgcaa caatatcggc
     3361 gatttcttca gtgtttcctg tcatgctagc atatacgatt tttgctaaag ccatgatgtg
     3421 cctcctcgtt tataattatt taacaattat gaattatacc aaaacatcat tgaaaagaaa
     3481 ataaaaccta aagacacctg gggaatgtca tttggctaaa tgtatgccgc tatgttaaaa
     3541 tgagagggat ttgtaaaaag gagacgaaaa tatgattact ttaaaatcag cacgtgaaat
     3601 tgaaatgatg gcagaatctg gagctttatt agcagatgta cataaaaatc tgcgtgattt
     3661 tataaaacca ggaatcacaa gttgggatat tgaggtattt gtcagaaact atatcgagag
     3721 tcatggcgga attgctgcgc aaattggctt tgaagggtat gaatatgcga cttgtatcag
     3781 tataaatgat gaaatttgtc atggattccc tcgtaaaaag cctttgaaaa acggtgattt
     3841 ggtaaaagtc gatatgtgta tcgatttgaa aggcggcatt tctgattctt gttgggccta
     3901 tgtagttgga gaatctacac ctgagatcga ccgattgatg gaagtaacga agaaagcttt
     3961 gtacatcggt attgagcaag cacaagtagg gaatcggatt ggtgacatcg gtcatgctat
     4021 ccaaacttat gtagaaggcg aaaatctagc gatcgttcgt gattttattg gtcatggcgt
     4081 tggtcctacg attcatgaag aaccagcagt tcctcattac ggagaagctg gaaaagggtt
     4141 gcggttgaaa gaaggcatgg ttatcacgat cgaacctatg gttaataccg gaacttggaa
     4201 aatgaaaatg gatcctaacg gctggactgc gtatacaaga gacggcggac tttcttgcca
     4261 atatgaacat acattggcta ttactaaaga tggtccaaga attttgacat cacaaggaga
     4321 agaaggcact tattgatcta tccagtcaat aggtacggga gaggtacatg aaagtttgga
     4381 acaaaataaa acaaaatgaa aatctgatgc gctttattga gacgactcaa aagcgcatca
     4441 ctgattcaga aatgagtacg acctctgtcg tcgttgctta ttatttactc ttgtctttat
     4501 ttccattgat tattgctttt ggtaatattt taccgtttct ccacatcgat caagagactg
     4561 tactgaccta tttacgagaa gtgattcctg agaccgttta ccagtttatc ggtcctgcaa
     4621 tcaaagacct tttgacacaa agttcaggag gcttgttatc cgtttctgca ttagcggctt
     4681 tatggtctgc aagtcaaagt atcaatgctt tgcaaattgc gatgaataaa gcgtatggcg
     4741 tagaaaatag aaaaaatttt ttgatcgtcc gtttcttctc actaattgtc attgtcttgt
     4801 tcatgattgc tattagtggg gtaactatcg ttcttgggtt gggacagctg attttggatg
     4861 ctattcagcc aatcgtacaa atacccgtga actttatcga tcagttccaa actttgaagt
     4921 ggccaatcac attggtagct ttatttgtca tcatgttctt gatttattta attgtcccta
     4981 atgctcagtt gaagttgaag acgatcattc cgggcacgat cttcgctact gtcggctgga
     5041 tgctgttatc acaagtgttt agtatctatg ctaggtattt tgcaacgagg atcagcggtt
     5101 atcagatcat aggaagtttt attgtactca tgctttggct taattttgct gctacgatca
     5161 tcattttggg cggaatcatc aatgcagttg ttcaagaata cgtgacgggt catcaaatca
     5221 aaaaacgtcg agatccaagc cgaaaatggt tcaataaatt gaaaactaaa ttttctcata
     5281 aaaaaaagta gaaacaagag ttcttattgt aaatttatat cagtaattta taataattat
     5341 gactcttaat taaagtaggt gcccatatgc ttgtttcttt tagtatggtt ataagatttt
     5401 ttctgacaat tttattatca ctgatattaa cacctgtatt caaaatcatt tcagtacaaa
     5461 caggtatggt cgacaaacca aatgaaagaa gaataaataa agttccaatg ccttcagcgg
     5521 gcggactgcc tatctttatt tcatttgtta tatcgacttt attcttattc cgtaaaatta
     5581 tccctcaatc gtatattgta ccgatcttga ttgcttcttt agtaatcata gtcacaggag
     5641 tgctcgatga taaatatgag ctgacaccta aacaaaaaag tatagggatc ttgattgctt
     5701 ctcttattat ttattttgta gcagatatac gtattgattc cttcacgctt ccttttattg
     5761 gatatatcca actaggctgg cttagttttc ccgtaacgat tttttggatc ttcggtatca
     5821 cgaatgcggt caatctgatt gacggtttgg atgggctggc ggcagggatt tcattgatcg
     5881 gtttaattac gataggaatc attggatatt ttttcctcca tgcttctaca gtgtatattc
     5941 cagttgttat tttctgtctt gtagcaagta ttatcggttt ttttccttac aatttttatc
     6001 cggcaaagat ttatttaggc gatacaggag ctctattcct agggtttatg atggccgtcc
     6061 tttcactaca agggctgaag aacgtgactt ttgtttcatc gatttctttg ctgatcgtca
     6121 tgggagtgcc ggtaacagac acgttttttg ctatcattag gagaaaagca aaccgtgttt
     6181 ctttttctac agcagataaa aaacatcttc atcaccgact aatcagttta ggctttactc
     6241 ataaaggtgc tgtcctgacg atttatgcga tggctttgat gttttctttc acagctatgg
     6301 tcatgaatta tactggaaga atcggaacga tcatattgat tattgccatg ttgtttgctg
     6361 caattttatt atttgaattg attggtctga tcaatgagaa acatcagttt attttaaaaa
     6421 cattgcgatt tttagggaat aaagagtacc gtacgcaagt aaaacagaaa tatttgaaga
     6481 aatggaaccg tcatatagat gaataacaaa ttaaaccagc cgatccttat tagtattgtg
     6541 acggcaaaca gtgataaaat ttttcggaca ctagataatt ttatttcaac tgtaaaagat
     6601 gaaacagcag taaaaatccg aatttttgac aacaattcac ttcccgagta caaaaaaaga
     6661 ctaacagagt ataggaaata tccctacatc gatatctatt ttcatgatga aaattccgga
     6721 tttggttatg gacataatca caatctgata gctagcgatt ctcgctatgg cattatttgt
     6781 aacccagata ttttagtgga tgaagagaca gttacagcat tggtcttgtt attgaaagaa
     6841 catccggaat gtgcgatgtt agcacctaag attcttaatg aagatggaac aactcagcac
     6901 ctcatccgtg agcagctggc tgtttttgat tacgttttgc gctttgtgcc tttcaaatgg
     6961 gtcaaaaaaa agtttgacaa acgactggct gtgtttgaat gccgaggatt gcctactgat
     7021 gctaatagtt atgtacggat gatctcaggt agttttatgg tagctgatat tgataaattt
     7081 aaagagataa aggggtttga tgagcgtttc ttcatgtact ttgaagacaa cgacttgtgt
     7141 ttgagttttg aaaaagcagg ttacaagtta ttgtacacac ccttttatgc agttatccat
     7201 ttttacggga agggtgcgca tcgaaatgca aaactgtttc gcgtgttttt gacttctatg
     7261 atgaaatttt tcaataagtg ggggtggcgt tttttctgat gaaaccagaa atattagtag
     7321 tcgttgtgct ttatcagcaa ctattcagtc aatctccagc ttatggacca ttaagcaaag
     7381 cacttagcga aaagcaaatt cagcttatta tttacgataa tagtcctcaa aggcagcaaa
     7441 atgagctgtt tgaaaaagaa aatatcgttt atcatcatga tccgcaaaat cctggtttag
     7501 cggtggctta taattttgcg ttgtctcaag cagatgaagg aacacgttgt ctcgtcactc
     7561 tggatcagga tacgaggcta gtcgacgatt attttgaact attgcgtaaa gtaaccttta
     7621 cagatgaatg tgttgctgca gttccgatga ttttttctgg gagccgacaa gtttctcccc
     7681 tatattcaga tcgttatatc aatcgtacag ctcaagcagt agaagttgaa ggcccaacag
     7741 ctgaacgaat catggcaatc aattcaggtg ttgcctggtc tgtcaaattt ctaaaaaaga
     7801 ttggcgggtt taatcctgct ttttctttag attttttgga tcattggctg ttttggaaag
     7861 tggatcagtt ggaaaaaact gtggagatcc ttcctgtacg aatggaacat gatctttcgg
     7921 ttttagacta tgaaaaagtt tcaagtaatc gataccgttc tattttacaa gcagaaaatc
     7981 gattttatca tgaatatgat caaaggcatt tatccagtca ccgacgacaa ttattgttaa
     8041 gaacagctaa acagttcttg accgtcaaaa accggcatat ctggcggatg actttgcgct
     8101 catatgttaa gaattggaag gtgtgaattc gtgcgttcgg tttgtatagc tacctttaat
     8161 ggaggcagat atttaagaga gcagttagat agtattttat ctcagctttc agcagaagat
     8221 gaagtagtca tttctgatga tgggtcagta gatgatacat gggatatatt aactgaatac
     8281 gcagaaaaag ataagcgaat ccagcttttt tcggggcctc gtcaaggatt gatcgctaat
     8341 tttggttatg cgatccagca gacgaaagga gatctaatct ttttggcaga ccaagatgat
     8401 gtttggttgg agacaaaagt gaaaaagatc actgactatt ttgagcagaa tccggatcag
     8461 ctagtagtag tttctgattt aatcattgtg gacagcgagc tgcaagagat ccatgattcg
     8521 tatttttcgt ttcgaaattc caaagaagga tggcttaata atgtgataag aagtcactat
     8581 attggtgctg gtatggcttt ccggtcttct ttaaaacccg atattttgcc gatcccgtca
     8641 gaagtgccga tgcatgatat gtggattggt ttgctgggag gaaagaagac tgggttccta
     8701 agagaaccgt taactctgta ccgaagacat gatttaaacg cgagtgagat tgaaacacat
     8761 tctaaattac ggcaaaaaat ttcttggcgt gttcatctga ttacggcgtt gatcaaacga
     8821 aaatggttta aacgttgata aaagatgttt aaatccttaa tatttccatg acaaatataa
     8881 caattgatga gataataaca aagtgaaatt tgaaatcaaa aaggagagca gatatccatg
     8941 aagggaatca ttcttgctgg gggcagcgga acacgtctgt accctttaac aaaggcaaca
     9001 tcgaaacaat tgatgccgat ttatgacaaa ccaatgattt attatccaat gtctactctg
     9061 atgttggcgg gaatcaaaga aattctgatt atttcaacgc cacaagatac accacgtttc
     9121 aaggaattat ttggaaacgg aaatgattta ggactgcaga tcgaatatgc agtacaagaa
     9181 agtccagatg gattggcaca agcgtttatc attggagaag aatttattgg agatgatagt
     9241 gtctgcttgg tattagggga caatatttac tatggtggcg gtctgtctaa aatgttgcaa
     9301 cgagcagctt caaaagatac aggcgcgaca gtatttggct atcatgtcaa tgatccagaa
     9361 cgctttggtg tagtagaatt tgatgagaat atgcaagctt tgtcgattga agaaaagcct
     9421 gctcagccaa aatcaaatta cgctgtaact ggcctttatt tttatgacaa tgaggtaatt
     9481 tctatcgcta aaggtatcaa accttcagaa cgtggagaac tagagattac ggatgtcaat
     9541 aaagcatatt tggataaagg aaaactatcc gtagaactta tgggacgcgg atttgcttgg
     9601 cttgatacag gaacacatga atcattgctt gaagcatcta cctttatcga aacaattgaa
     9661 aaacgtcaga acttaaaagt ggcttgtttg gaagaaattg cttaccgtat gggctacatc
     9721 gataaggaac aattgctggc ccttgcacag ccattgaaaa aaaatcaata cggtcaatat
     9781 ttattgaatt tggccgctga ataaggagtc cttacagatg aaagtaacag atacaaaatt
     9841 aacagatgtc aaaatcgtag aaatggacgt ctttggcgat catcgcggtt tcttcacaga
     9901 aagttattca aaagcaaaat ttgcagaaca tggattgaaa tatgactttg ttcaagacaa
     9961 ccattcacta tcagctgaag ctggtgtcct tcgggggctt catttccaaa aaggagaagc
    10021 tgcacagaca aaattgattc gggtagtcac tggagcagtt ttagatgtag tagtggatat
    10081 ccgcaaaggt agcccgactt atggtcaatg ggacggattt atcttatctg aacataatca
    10141 tcgacagttg cttgtaccta aaggatatgc ccatggattc gttactttga caccaaatgt
    10201 gaacttcctt tataagtgtg ataattacta taatgcagaa gcagatgggg gcatcgcatt
    10261 tgatgatcca gatttagcga ttgattggcc aattgatcca tcaagagcga ttatgtcgga
    10321 aaaagatcac cgtcatccga cattaaaaga atttgaagca gaaaatccat ttgtttacgg
    10381 agaaatctaa gaattaagga gaaattttct tatgaaaaac atcattgtta ccggaggtgc
    10441 cggattcatc ggctctaatt tcgttcatta cgtggtaaac aatcatccag aagtgcatgt
    10501 cactgttttg gataaactga cttatgcagg aaacaaagag aacttggccg gacttcctag
    10561 cgatcgcgta gaattggtcg taggagatgt tgcagatgca gaattagtgg accgtctagt
    10621 aaaagaagcg gacgcagttg ttcattacgc ggcagaatcc cataacgata attcattaaa
    10681 agatcctttc ccatttgtac aaacgaatat cattggtacg tatacacttt tagaagcttg
    10741 ccgcaagtat gatgtacgct atcatcacgt atctactgac gaggtttacg gcgatcttcc
    10801 attacgtgaa gacctgccag gacatgggga aggcgaagga gaaaaattca ctgcggaaac
    10861 accatataat ccttcaagtc catattcttc tacaaaagct ggttcagatc tgttagtaaa
    10921 agcatgggtg cgttcttttg gcttacgtgc aaccatttca aactgttcaa acaactatgg
    10981 tccttatcag catattgaaa aatttattcc tcgccaagta acgagtatct taagcggtat
    11041 tcgtccaaaa ttatatggcg aaggaaagaa tgttcgtgac tggatccata cgaatgatca
    11101 ttcttcagca gtgtggctga ttttgacgaa aggcagaatt ggcgaaacct atctgatcgg
    11161 tgcagacggt gaagaagata ataagaccgt gatggaattg atcttagaaa tgatgggcca
    11221 accaaaagac gcatacgacc atgtaaacga ccgcgcaggg cacgatttgc gttatgcaat
    11281 cgattcgaca aaattgcgag aagagctagg ttggcagcct gagtttacga acttccgtga
    11341 tgggttggct gacacgatca aatggtacga agaacatgaa gactggtgga aaaaagaaaa
    11401 agaggccgtt gaagcagcat atgccaaaaa tggacaataa tacagaggaa atttcactgt
    11461 attaatggaa aaacacatgt gaggcaggcg ctcgcctgtc tcttttttaa aataaaaatc
    11521 aaggagcgga taaccatatg tttttattaa ctggaggaaa tggacaatta ggtacagaat
    11581 tgcgccattt attagatgaa aaaaatctgc catatgtttc aacagatgca aaagacttgg
    11641 atatcacaga tgctgagaaa acaatggctt atattacaga attaaaaccc gatgttattt
    11701 tccattgcgc agcttacaca gcagtagata aagcagaaga agaagcaaaa gaattagatg
    11761 agaaaatcaa tgtcgatggc acaagaaatg tagcacttgc agcaaaagct gtgaatgcac
    11821 aattcgtcta tatcagtaca gactatgttt tcgatggaaa aaacaaaaat gtagaatacg
    11881 aagtagacga ccaaacgaat ccactaaatg aatatggtcg tacgaaatta ttaggcgaaa
    11941 aagcagtaga agagatttta gaagactatt atatcatacg aacttcttgg gttttcggga
    12001 tctatggaca caatttcgtc ttcacgatgc aacgcctagc ggaaacccgc gacaaactga
    12061 ctgtagtaaa tgaccagttt gggcgaccaa cttggacacg tactttggca gaatttatgg
    12121 tatatgtcat cgaagaaaaa gcaccatttg gtatttatca tttgtcgaac gaaaatagct
    12181 gttcttggta tgagttcgca aaagaaatat tgaaagatac agatgtagaa gttgcacctg
    12241 ttacgtcaga agaatttccg caaaaagcaa cacgtccgca gtattcagtc atgagcctga
    12301 aaaagacaga agcgttaggt tttgtcatac caacttggca agaagcactt gcgcaaatgc
    12361 tggaagctgc caaaaaaatg aataagtaat ttccttttta tcaaatagaa tttggagcat
    12421 gatatggaaa aacaaacaag aagtgcacga cgaaaagaaa agccaaaagc gaatcaggca
    12481 gattcgaatt tggtgcttga cctgtttcta aaactccgct atcttattgg cgttttagtc
    12541 atcgtggtca tcgttacttt taatttaaac ggaagttcac ttggtgtttg ggataaatat
    12601 gtgtcgcaaa gagacgatgg gaaaaaaact gatgtgattt ttggacagaa tcgggaagtg
    12661 cggtcagacg aatggctggt gcagacacct ttttacctct cacaagctga agaggacttt
    12721 tcgttggtga acgaggatta ttcattaaac ggacaaaaca tgatcattgc gtataattct
    12781 cctgtaaagg atattacggt tatcggaaaa ccattcaact ggggattctt tttcctcgga
    12841 agagatcgag gactttcctt ttattgggcg atgaaattag tcgtcatggt cttgcttgga
    12901 ttcgaagtac tgatgatatt aacgaagagg aaaaaagctc tatcgctaat cggtgcattt
    12961 gcgctgacat tttctccagc agtccagtgg tggttcatgc aacatgtggg cgatttgatt
    13021 ttctttactt tgggactgat gattgctttt tatcattatt tctacaatca tgataaaaaa
    13081 tggctacgaa cgttgatgat gcttttggtg gttattttcg gtattgggtt tattttagtg
    13141 atttacccag cccatcaagt gatgctggcg tacttgctga ttttttattt tgttggcctg
    13201 cttatttact atagcaagaa agtttcatgg gatctgttgg atgtagtgtt gattggaggg
    13261 gcagttttat ttattggcgg agtcatggtt catttctggc taacgtcaaa agacgcattg
    13321 ttagcttctt tgaatacctt atatccagga aaacgggtaa gtactggagg aaagtggacg
    13381 attggcgaat ttttttcttt ccttacaaat tggaaaattc cttttgaaga tatcaactat
    13441 tcgaataact ctgaagtggc cctgttctat cacttttttc caactgtatt cctagcaagt
    13501 ccatttgtgt tatttggcaa aaaagatagc gaacaaaagt tactgggacg tattttgatg
    13561 attttttgtc tgttttctat cttttggatt accgttgggt tgccaaaatg gctagcagaa
    13621 atcactctgc tgtcttatgt tcctccatta agagccatcc ttacttttag ttttgcggct
    13681 tgtttattat cgatctggtt tatcgcttat atttggagag aagatgttat tcctatttgg
    13741 tataaattca ttttgttggc agtaaatgca tggtcttatt tctatgcgat tacgcattcg
    13801 aaaatggata attatttttc ggattttgaa accatcgcag ttgctgtgtt gggaacagtg
    13861 attttagctt ttgcattatt tggctggaga cgcttgtttt acctcgcttt tgtcgcctta
    13921 gttatcgtat ctggcttttt cgtcaatcca gtcgtagaag gtacaggagc gatttttgaa
    13981 aaaacactgg caaaagaaat ccaaaaaatc gatcaacaag atcctggtaa agtttggttg
    14041 tctgaagatg aattatataa cttcacccca acattgggag tgaaatcctt caatacagta
    14101 cgcttttatc cagatatagc tgcatggaag aaaatcgatc cgaaagaaga atacgagaag
    14161 tattataatc ggtatgctca tacaagagct tttatcgcta aggaagatac aaagttcatt
    14221 ttagatcgtc cagatatgtt ttcggtcacg ttgaattttg aagacgcaga aaaattaggg
    14281 atcgactatg tgatcagtaa acgaccatta actgattaca accaattata tgaagcgcag
    14341 ttcacgcagc tatatggacc ggataaagac ggttatatga tttttaaagt agaatatccg
    14401 gattatccag atgtaccaca aacacaaact caggaaaatc agctagtcgt acagtaatag
    14461 gctagctgag aataatcctg aaatattagt agaaaggaga gtgaagataa ttcaagcaat
    14521 cgtacaaaaa tttgtcaaca aaatgagtgt cggtttcttg cttttgttgc tgacgctagt
    14581 cttttttgtt tggacagcag gggtaggttt tccagcaact ttgtttatat ataacaatag
    14641 ttcattactt atcgctttta gtgtggctat tttgctcatt ttaaatgttt ataaagcaaa
    14701 aggcgtcgat tttctttatc taggtgttgc gcttgccctt ttcattttct tttcttactt
    14761 tcgggaatta agaaatgagt ctttatttgg agacttactg tttcctttga ttattttatt
    14821 ttgtttagta attaaatgga tcgagtggac aaaatgggat cgcggtattt atctgattgt
    14881 ctttacggtc ttcctcggaa tcacgttata tcgtgtattt acagagatcc aagtaccaga
    14941 aggacaaagc atctgggctc caggcaattc gctttctgat atttggatca atacgaatac
    15001 aatcggaagt tctttgatga ctcttgcttt attgatcagt ggatttgcca gttcttttga
    15061 acgatggtat gttcgaatca tcggtgggat agcagtcgtt gcagcctttt taggtatttg
    15121 ggtctgccaa tctagaggtg cattagtcgc gttgattgtt tttgcgatat tggatctttt
    15181 acctaaatcg ttgatgcgtg tcattcgtgc gccatttatt gcctacacgg ttacgattct
    15241 tttagctttg ccgatttctt atctggcggc tgtctctgaa aaagtcaatt tgtttaccgg
    15301 tcgagaagat atttggcata aattctatca gacgctagga gaaaaatctg aacaagtttt
    15361 actaggaatg aaaacattta tcttccagcg aggaaaccaa tttttaggaa accacaatag
    15421 ttataattct attttgaata tctatggatt gataggattt gggattgctg ctttgctgct
    15481 tatccttttt attggtcgct taacgttgaa agcagattta tccaacgggc agatgacttt
    15541 tatctgggcc ttttttgcgg tgatgatgca gtcatttatg gaggatacat tgacttcata
    15601 cccatgggtt ccgattattt atatcctgtt ggctatggcc gttcatcgat atgatgaacc
    15661 agaagttacg gaggaattct atgttatgga agaggcatat gacgatatga cttatgatga
    15721 tacatcttat gaggaacaac cagatagcgc tcattcttcc aggatccaac gctatcattg
    15781 ataggcacaa aatacgagag aggctgtgac aaccgtcgca gccttttttg gtttattgca
    15841 attgtctagc atcttctgta aaatagaaac gttagagaaa atcttgtatc atagaaaatt
    15901 aagaaagaaa taagaacgca gctcggtacc aaaaatatgg taaaatggca cttgactgta
    15961 attcactgaa aatcgcttgg aggaacacaa atgtttaaag atttgtattt atttatgaag
    16021 gacatttatc agaacagaaa attactgcta cagttttcga tcaatgactt caaatcaaga
    16081 tatgcaggat cgtttttagg aatcttatgg gcgtttatca atccgctatt tactgtattg
    16141 atttattggc tggtatttgg tgttggactg aaagcacaaa tgatcgatgc aaaataccca
    16201 tttatcgtat atttagttac cgggatgatt ccctggttct tcttctcaga tgcatttgca
    16261 tcaactacgg ttgtttttcg ggaatatact tatttagtaa agaaagtcgt attcaatatt
    16321 cgtatcttgc ccacgacgaa aatcctatcc aatctgtaca cccatttatt cttcattctg
    16381 attggcatag ctgtcgcgct tataaatggt atatatcctt cagcaatgtc tttacagctg
    16441 atttattatt tattttgcat gtgtgctttc ttgacaggat tgacttggct gacggcatcg
    16501 atccaaccat ttttacctga tatcatgcaa ttaattacga tcgtgctgca gttgatcatg
    16561 tggacattac cgatattatg gagccctaat cagtttgatt caggtcttgt aagcatattg
    16621 aaattaaatc cgctgtacta tgttgttcaa ggttatcgtg agtcattttt aagtgaagct
    16681 tggttctggg agcatggaac atatactttg tatttctggg ttttcacgtt agtaatgttc
    16741 gtaatcggct ctgcagtgtt ccgtagattg aaaccacatt tttcagatgt gctgtaagga
    16801 gaatagctat gacagaatat gcaattagac taaaagatat cacaaaatct tataatatgt
    16861 acgcaaaacc ttcggatcga tttcgtgaag ccctcaatcc ttttaagaaa tcctatcatg
    16921 atgtatttta tgcgttgaaa aacgtgaatg tagaaataaa aaaaggtgag atgatcggtt
    16981 tcgtgggaga aaacggatcg ggaaaatcta caatgttgaa aatcattacg ggagtactga
    17041 caccaacatc cggcacgatg gaaatcgaag gaaaaatctc agctttgcta gaactaggat
    17101 caggattcaa tcctgagtat tctggctatg aaaatatcta tttaaatgga atggttctag
    17161 gattttcaag agaagaagtc gatgaaatgg tcgatgatat cattgaattt gctgacatcg
    17221 gcgaccatat ttatcaacct gtaaaaacct attcaagtgg gatgttcgtt cgtctagctt
    17281 ttgctgtagc gatcaatgtg aatcctgata ttttgattgt ggatgaagca ttggcagtag
    17341 gagatcttga atttcaacta aaatgtatgg aaaaatttac agaaatcaag aactcgggaa
    17401 agacgatttt attcgtttct catgatatta gttcgattcg ccgtttttgt gatcgttcct
    17461 attggttgaa aaatggaaaa gttgtcgaat atggtgatac gatggaagta acgacgaatt
    17521 acgaaaactt tttgaagaaa aaatcagtta aaacagtaga tcgaaataaa aaccaagtag
    17581 aacgcttcgc agctccagat atcgtagacg tgacgaaagc cgaattgttg gataaagata
    17641 aaaatccagt ggatatgctg gaacaaggag aaaaactata cgttaaagta acctatgaag
    17701 taaaagacga tacaatcaaa aatccagttt taggaattgc tctgcgtaca gtcgacaatt
    17761 tttatgtgtg cggactcaat actctactag atggtatcaa gatcccttgg aaaaaaggtg
    17821 agaatatctt ttatctggag tatgaaaagt taggtttgtt agctggtgaa tattattttg
    17881 acgttgcggt ctttgaagaa aatgcaacgg ttcctttggt atacaagaca aaatacatga
    17941 acttgtttgt cagcggttct tatatcggag aaggcatcgt ggtcttagac cataaatggg
    18001 aagaagggac tcatagcaat gaaatatgat tttgaaatgg acctagatga acaaagcagt
    18061 gtgggaaaaa ttgccgcaca gatcaaacca ggaagtaaag ttttagagtt tggtcctgga
    18121 aacggccgat taacgaaaca tttgatcgga gcaaaacaat gtgaagtgag cattgtcgag
    18181 ctagataagg aattgttcga ttttgtcagc gagtttgccc aagatggatt ttatggagat
    18241 atcgaaagtt ttgaatgggc aaactattat gctggacaaa catttgatta cgttttgttt
    18301 gccgatgttt tagaacattt ggtagatcct ggaaaaacgt tgaagaaagt tagagaattt
    18361 ttgaatgaag aaggagaaat tctaattaca ttccctaacc ttgcgcataa ttctgtcatg
    18421 attgatttgt tcaataatca attgccatgg gcttcttatg gtttgctaga tgaaacccat
    18481 aattcttttt atactcatga tggcttccaa aaagtttttg aaaaagcagg attatacatc
    18541 aatattgaag attatttgta cttagctgta ggtgatacag aattaaaatc aacttatgaa
    18601 gaattgccgg aagctgtacg ttatgatttc aaaatgcgac catttggcga agtctaccaa
    18661 tacttcttct cattgatgaa acatccagta gcacaaagtt ccattgcgca gccgcaaaat
    18721 tctaattatg tgaaagtatt agaagtaatc cagcaaacga aacaagatga aataattcaa
    18781 caaataccat tcaataattt cacaggggaa aatgagacac taagtttcca tgtatcagaa
    18841 ttagcagaac gaatggtgtt tcgcttcgcg cagcaaccta gctttatcga attcagcgca
    18901 gaagctgctg gagaaaaact cacatttatc gattcaaatg ctgtagtcaa aacagtaaat
    18961 gattgttatt tgtttgatgg taaagattta ccagagttct ttttaacgga tattgcaggg
    19021 aaagaagtga ctatccattg tcactatcgt tttatcggag aattgacacc aacaatgaaa
    19081 gaattgctgg aaacaatccg tccaatggct gaagttacaa agcagctttc tgagaaaaat
    19141 gatgaattag aaagagaaaa tcattatctt attgagaata atacaagatt agatcagacg
    19201 ttgaaaacaa ccataaatcg ctattgcacg ttgttggaaa gtgatgaatg gacaatcaaa
    19261 catagattag ggcgacgtaa gaaagaaaca tcgaaaaaaa tccaagaaaa agagctttct
    19321 ctatgtattg acgaaaaaat ctgggatgca gaaacaaaga tattgaaaat catcgggtgg
    19381 ggaatcgcta attcggatcg ccaaccattg tcctataagt tatcagccga ccaatcacca
    19441 ttttttgaag caattcctgt tttacgagaa gaagtcaatc aagcaaaaca gctagctgaa
    19501 ggagcaaaag caggatttga actgcggatc ctttgcgaga aggaacgttc attcttagtt
    19561 gaagcagtgg ctcaaaatgg tcaatcatgg atcattgaaa tgtaaataat gattaactta
    19621 taaagataag gagtaaacga atcgtgcaaa aaaaagaaat tgccgtattt atcgatcaaa
    19681 tcactcgtga acgggccact ggcgatttgc ttattgtggg ctgggcaatt gatgaagtga
    19741 ccaaagaaat ccctacgata aaggtagaaa aagaaaatgt gattgctgaa gcaacacatg
    19801 tcgtaagatt ggatatcaat catttatata atcttgatgt taagacccaa agcggtttta
    19861 aggtacgttt atcagggaaa atgagaggaa aagcaatctt agacttccaa acagcaagac
    19921 atcaaaatgg catagctgtg aaattaaacg gtaaatatcc ttatgatgac ggcattgaat
    19981 cttcatggga aagaaaaaaa agattactga aaaaagggat aaattatgca aggactcatg
    20041 gcgtaaaaaa agcaatccgc cgggtcaaac tggaattgaa accaggatcg atcgactatg
    20101 cagagtggat cagtcgtcat gagaaacctg atctgaaaga gcaaaggaaa gaagtacaag
    20161 gatttgccta ccgcccattg atttccatac ttatgcctgt ctataacgtt gaaatcaaat
    20221 ggctcgaaaa atgcattgat tcggtacttg atcaaacgta tgatcactgg gaactatgta
    20281 tcagtgatga tgcttctact gatccagcta tccgaaagtg tctagaatcc tatcaagcaa
    20341 aagatgatag aattaaagtc gttttccgac aagaaaatgg acacatcagt ctggcaacta
    20401 attctgcatt ggaaatggca gaaggagagt ttatagcgct cttagataat gatgacgaat
    20461 tacctccatt tgccttatat gaagtggcaa aagtgctaaa cgtacatcca gaactggatt
    20521 tgatctacag tgacgaagat aaaatcgatg cagatgggaa tcgctttgac ccgcacttca
    20581 aagcagactg gtctcccgat acattgatgg gaaataatta tatctctcac ttaggcgtat
    20641 atcggacaag cattgttaag gaacttggcg gtttccgtaa aggatatgaa ggctctcaag
    20701 attatgattt ggttcttcgt gtgacagaac aaattcctgc agaacacatt taccacattg
    20761 accgtgtctt gtatcattgg cggacgatcc cgggatcaac tgcaagcaat ggagaagcta
    20821 aaagctacat ctatgattct ggagtgaaag cattgacaga tgctctatct agaagaaata
    20881 tcaagggaag cgtacatcct ggcagaattt ctggttttta tgaaatcact tatgatgttt
    20941 tgcaagaaga cttggtcagt gtgatcattc caactaagaa tggctatgaa gatctaaaaa
    21001 cttgtgtgga ttcgatcatc gaaaaaacgt cttatccaaa ttatgaaatc attattgcag
    21061 ataatggaag tacagatcct aaaatgcaag aattatttgc agaatacaag catcagctga
    21121 aagaccgatt tatcgtcgaa ttaattgaca ttccattcaa ttattcacgt atcaacaatc
    21181 tggcagcaga aaaagcaaac ggcaaatact ttttattctt gaataacgat acagaagtga
    21241 ttgaacctga ctggatgaca gcgatggttt cttatgcaca gtttgatcga atcggctgtg
    21301 taggagcaaa attattttat ccggatgata caacgcagca tgcaggtgta ttattaggga
    21361 tcggtggcgt tgcaggacat gcattgaata actatgatcg aacacattgt ggttactttg
    21421 gccgattggt cattgacgtc aattacttag cagtcactgc agcttgtatg atggtcaagg
    21481 cagctgactt caatgctgtc aatggatttg atgaaatatt agaagtagca ttcaacgatg
    21541 ttgacttgtg cttgaaagtt tatgaactgg gtcgttacaa tgtctatgca catcaagcag
    21601 aattgtacca tttcgaatct aaatcaagag gttatgaaga tacaccagaa aaacaaagac
    21661 gttttgctgg ggaaatcaaa aaaatgcaag ataagtggcc ttcttatatt gctcatgacc
    21721 cattttataa cgataatcta actaaagaag gaatcggcga tttttcgttg agacctgatt
    21781 gattaaaaga actgtatttt catttttatt aatgaaaata cagttctttt aatttaa
//
LOCUS       DACEKH010000041        21576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEKH010000041 DACEKH010000000
VERSION     DACEKH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.042"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(541..1566)
                     /locus_tag="IW662_001453"
     CDS             complement(541..1566)
                     /locus_tag="IW662_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0866284.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1841..3526
                     /locus_tag="IW662_001454"
     CDS             1841..3526
                     /locus_tag="IW662_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ0866285.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3540..4823
                     /locus_tag="IW662_001455"
     CDS             3540..4823
                     /locus_tag="IW662_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0866286.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4893..5762
                     /locus_tag="IW662_001456"
     CDS             4893..5762
                     /locus_tag="IW662_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0866287.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5759..6595
                     /locus_tag="IW662_001457"
     CDS             5759..6595
                     /locus_tag="IW662_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0866288.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6621..7688
                     /locus_tag="IW662_001458"
     CDS             6621..7688
                     /locus_tag="IW662_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ0866289.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7685..8512
                     /locus_tag="IW662_001459"
     CDS             7685..8512
                     /locus_tag="IW662_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0866290.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8557..9582
                     /locus_tag="IW662_001460"
     CDS             8557..9582
                     /locus_tag="IW662_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0866291.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9579..11762
                     /locus_tag="IW662_001461"
     CDS             9579..11762
                     /locus_tag="IW662_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0866292.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11762..12892
                     /locus_tag="IW662_001462"
     CDS             11762..12892
                     /locus_tag="IW662_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0866293.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12889..13566
                     /gene="pgmB"
                     /locus_tag="IW662_001463"
     CDS             12889..13566
                     /gene="pgmB"
                     /locus_tag="IW662_001463"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0866294.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13650..14177
                     /gene="msrA"
                     /locus_tag="IW662_001464"
     CDS             13650..14177
                     /gene="msrA"
                     /locus_tag="IW662_001464"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0866295.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14378..15064)
                     /locus_tag="IW662_001465"
     CDS             complement(14378..15064)
                     /locus_tag="IW662_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ0866296.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15144..16682
                     /locus_tag="IW662_001466"
     CDS             15144..16682
                     /locus_tag="IW662_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ0866297.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16802..17269
                     /locus_tag="IW662_001467"
     CDS             16802..17269
                     /locus_tag="IW662_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0866298.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17788..18231
                     /gene="rplM"
                     /locus_tag="IW662_001468"
     CDS             17788..18231
                     /gene="rplM"
                     /locus_tag="IW662_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ0866299.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18247..18639
                     /gene="rpsI"
                     /locus_tag="IW662_001469"
     CDS             18247..18639
                     /gene="rpsI"
                     /locus_tag="IW662_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0866300.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18728..19954)
                     /locus_tag="IW662_001470"
     CDS             complement(18728..19954)
                     /locus_tag="IW662_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0866301.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(20026..20184)
                     /locus_tag="IW662_001471"
     CDS             complement(20026..20184)
                     /locus_tag="IW662_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0866302.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            20872..>21576
                     /locus_tag="IW662_001472"
     CDS             20872..>21576
                     /locus_tag="IW662_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866303.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
BASE COUNT         7002 a         3823 c         4488 g         6263 t
ORIGIN      
        1 gtctaaaata atttgtctaa ctagaagaaa agtgggaaag gatcttaaca acatcggttc
       61 gtatccaatt aaggtatcgg aacgatcatc ttccaagaga agtcaatcaa caaaagtaaa
      121 gatagatcat tttttgaatg caaaataaat tgatgggaag atagagcggt tatttttaat
      181 attttgctag ctattattga ataattcctc gaataaaaca aaaccagtag acatgtctgt
      241 tattctattt atgttttaga gataagtatc cccaaataac atggattgaa ataagggtaa
      301 atgtgtttaa aaaaacgaac tataaaaact tatacacaac gtggttaaaa atagtttttt
      361 cttccctgtg gaaaaaatag tttaacacaa acattatagt tatccacagc tatctaaaac
      421 agatggaaaa taagaaaaat tcgctaaaat accgggaggg ggtaacgagg gatgagtgat
      481 tgaaaaaaga acagagtaac tgacttatcc tcagtgtttc tgttctttta taattctgtt
      541 ctacatttct tgttgttgga tcgaatctgt gagagtaatc tcggttgaaa gaatgattct
      601 tttttcttca gggtattggt ggtggattag ttggaataaa agttctgtgg ctttgattcc
      661 taactgattt gtcggaatgt ctatccgagt caactgtggc gaaaataggg aggacaataa
      721 gggatcactg aatcctgcga tcgaaagatc atcaggtatc tgctttccag attgttctgc
      781 cactttgtaa atactcaatg attttaatgc atcggtagaa aaaatcgcag ttggtctttc
      841 tcgtagcttc attaaatcat tcattgctgc catgctatct tctaagcgaa agccgccgtc
      901 tataatccat tcattacgga tatctaggtg gtgatcaaat aagcattgac ggtaaccatt
      961 gagacgatca ttggatacat aaacttctgt cggagcatgg agacagccga tatgacggtg
     1021 tccattcttg atcagtgttt ccaccaaatc gtaactatcc tggaaattat ccgtgtccac
     1081 tgagtagatg ttcgtgtagt cgtttccgac ttttccgatt accacaatag gaatattgta
     1141 ttgatccaat tcaaaaagaa gtgtttcatc catcgtagag cttaaaataa taattcctcg
     1201 gatcattttt tcttggatct ttttcgcgca tttttctagc tcttcttctg gtgtttttgc
     1261 cgtttgtaga atcaagtcga agttattttg ttcagaaacg gcagaaatgg attgaataac
     1321 atctgaaaaa aaaggattgc cataggtcgt atgagaagta cgatgagata atgctagaat
     1381 cgcgtcaaaa ccatttcctg acagtcctct tgcaattttg ctcggttcat aattcaattc
     1441 tttgattgct tgaagtacac ttgcttttgc tttatcagag atatttggct ggttatttaa
     1501 tactctggag acagttgatt ttgagacatt agcttttttc gcaatgtcat aaatagtagg
     1561 tttcaatttt atatgcacct cctttttggg agcgttttag aataattcca tagtacaata
     1621 gacattcttt tttgtctatg attttgaaaa tttaggaata gcttgcatga tcatgagaga
     1681 ccatattttt ttaagaagct attaattatt gatacataag ctttcttatt tgtatttttg
     1741 tgtatatcaa aattatttaa aaaaatgcta ttgacagtaa gcgttttcca taatatgatc
     1801 aaatcgttgg gaacgctccc aaaaaagaaa ggggaatccg atgtatacaa aacttttaca
     1861 agcgatttat cgcgactcag atttgtctga gaaaaccgcc tctatgattg aacaaaaagt
     1921 cagtgtttat cagcagaaga aaggccaggc aggcaaactg cattgggacg aagaagatat
     1981 tttcttgatt acttatgggg atcaattttt tgaagagcag caaccaactt tgcgtacatt
     2041 taagaaattt tatcaaaagt atttggcaga tacatttaga gttgtacatt tcttgccatt
     2101 tttcccatat acatctgatg atggtttctc cgttgtaaat tatcaggcaa tcaacgaccg
     2161 tttgggcaca tgggaagata tagaagaaat gagaagcgaa acacggttaa tgttcgactt
     2221 tgtctgcaat catatgtctg ccgaaagtac ttggtttcag gaatatttaa acttgaatga
     2281 tacgtacaaa gattttttta ttgatttgcc actggataca gatacaagca gcgtgactag
     2341 accaaggacc agcccgttac ttacaacgtt cattgacaaa aacgggaaag aaaaacacgt
     2401 atggacgact tttagcgaag atcaagtaga cttgaatttt aaaaatccag aagttttatt
     2461 gaaaatgatc gatattcttt tgttttactt agaaaaaggc gcttcattta ttcgcttgga
     2521 cgctgtggga tttttatgga aagaactagg aacttcttgt atacacctgc cagaaacgca
     2581 taaaatcatc caattattcc gtgcaatcgc tgatgaagca gctccaggaa cggtattgat
     2641 cactgagaca aatgtgccac acaaagataa tatcagttat tttgggaatg gcgagaatga
     2701 agcacagatg gtctatcagt tttcgctacc acctttgacc cttcaagcga ttcgttcggg
     2761 taatacaagt aagttgaaag aatggttaaa aaccatcgat ttcgcatcga aaaaaacaac
     2821 tttttttaat tttttagcct cccatgacgg tatcggattg aatccggtac gaggtattct
     2881 gccggaaaaa acgatccttg aattagtgga acagctgaag aaagagggtg cccgagtaaa
     2941 ctacaagaaa aacccagatg gaacgaccag tccatatgag ataaacgcta cgtatctaga
     3001 tgcgttaagt tctccaacag atacgacgga atggagaagc aaacgctttt tagctgctca
     3061 ttcatttttg gtaacgttac caggcgtgcc ggcaatttat attcaaagtg tgctgggaag
     3121 tcgaaatgat tacaaaggcg tagaaaaaac aggagagaat cgaaaaatca atcggcaaaa
     3181 atttttgtta gcagatattg agaaagagtt gcagacagat ccattaagaa cagcagtata
     3241 tggtgaattg acccagatgc tatctattcg aaaaaaagaa aaagccttcc atccgaatag
     3301 tttgatttac ttactggact cagcagattc ttttatcagt tttattcgcg aagcgaagga
     3361 agagaaagtc ttagttatcc ataatttttc caatcaatca gcaggttatc ttttaccaaa
     3421 aggagattac ctagacctgt tttctggaca aattatccat tgcagtcaag aacgaaagtt
     3481 gatattccag ccgtatgaat atatgtggct gaaaataata aaataagagg tggaaaaaaa
     3541 tgaaaaaaat tattgttact acctgtctgc tagctactgg atttcttctt actgcttgtg
     3601 gtgaaagtca aagctctaac gaatcacaac agacaacgat tcaattcatg cactcttctg
     3661 tcgaacagga acgattaagt gtgatcaatg aactggttgc tgattttgaa aaagaaaatc
     3721 cagacatcaa aatcaagcaa gtgccggtag aagaatcagc gttcaataca aaaatcgtta
     3781 cgttagctcg ttccggtaaa ttaccagaag taatggaagt cagtcaggat tttgctaaag
     3841 tgatggataa agatgaactg attgatcaga cggcagtcca aaacgttatc gaggaagctg
     3901 gagaagataa ttattatgac ggagccaaaa accttgttcg ttcagaagat ggaaaaagct
     3961 atattgctgc accaattagc ggctgggttc aaggtatttg gtatgacaaa gaaaaattat
     4021 cagaagctgg tttttcagaa ccagaaaatt gggatgatat tttaaaaatt gctcaacatt
     4081 tcacagataa agagaataaa caatatggaa ttgctatgcc tactgctgaa ggaacaatga
     4141 gtgagcaagc gttctctcaa tttgctttgt caaatggagc aaatgttttg gatgatgaag
     4201 gcgaggtaac gatcgatacg gaaaagatga gagaagcatt gtcgttttat caaaacttat
     4261 cccaatacac gatgccaggc tctaatgatg tgacagaaat caaagatgct ttcatgaatg
     4321 gaacagcacc aatggcaatc tattcaactt acattttgcc ttctgtttat gaagcgggga
     4381 actcagaaaa tatcggtttt gctatcccaa cacaaaaaga tcaagctgtc tatggcacag
     4441 tatctggatt gacgatttct tctggtctag acgagaaaca aaaagaggct gctgaagcat
     4501 tcacagcttt cttatctcaa ccgaaaaaca tggaaaagtg ggtcttgatg tctcctggtg
     4561 gtgctcaacc agtgaataaa caagtagtcg aactggatgg ttacaaagaa aatgaagtca
     4621 tcaagtcttt tggcgagctg ccatctgaga ttgctagtgc ttttgatgaa gtacaagtct
     4681 ttggactagt cggggaaaag aatttcacga aaatgggaga tatcacaagc tcaggtgtga
     4741 tcggaaaagc agtgaaccaa gtaactgtcg gaaacgaaga tgtcgatcaa gctttagctg
     4801 acgcacaaaa aaatacgaaa taacaagaag ttctgccaat ggtgttcgaa aaaagtagat
     4861 agctattggc agattatcag ggggaggttt caatgaatcg tccaaggaaa cattctgact
     4921 taaaattagc catgggactt ttagcaccaa gcatgctttt attagctgtt ttagtgctga
     4981 ttccaatgct ttccaatatt cagatcagtt ttttcgatat gccgctgaat cctaactcag
     5041 acgccgtttt tatcggatta agcaattatc ttgaaatttt aagtgatagt gaattttatc
     5101 gttctttagg tttaacagtt gcttacacat tcttagttgt agcaggcagt acagctttag
     5161 gattagccgt agctattttg cttaaccgca agttcagatt taggaaaacg gtgcgttcac
     5221 tgattttatt aagttatgtt acgccatcta tttctttgat ttttgcttgg aaatatatgt
     5281 tcaataatag ttatggaatc gtcaatgatc tgttagtaga taggttgcag cttttcaacg
     5341 aagcaccgct gtggttcgac cagccagttg ctagcttctt tatggtcgtt ttgtttgcta
     5401 tctggagata ctatccgtat gcgtttattt catttttggc tattttgcaa acagtcgaca
     5461 aaacgcttta tgaagcagct gaaatggacg gtgcaaatgc ttggcagaag ttcaagatcg
     5521 tgacactgcc ggcaatcaaa ccagtactag caactgtgat tgttttacga tctatctgga
     5581 tgttctatat gtttacagat gtctatctac tgacgaataa agtcaatatc ttaggcgttt
     5641 atttgtatca gacagccttt gcgttcaatg atcttggaaa agcagcagct atatcgattc
     5701 tattgttcat cattattgca gcggtcgtct tgctctcacg aaagaaggtg gacttgaaat
     5761 gaaaaataaa aagagcagga aaatcttatt ttacgcgacg attttgttgc tattagctgt
     5821 caatttgttt ccatttttga tcatgctgat gacatcattc aaggattcac aagaagcaat
     5881 tgcaacgaca caaactattt ttccgaaaga atggactttt gaacattatc aggatatttt
     5941 caatgctgag atttttccat tcatcatgta tttcaaaaat agtttagtga tctccttgat
     6001 agcctccgta actgcagtag tgattgggat cagcggggca tacgctttgt caaaactacg
     6061 cttcaaagct cgctcgaaaa tcaatgctag cttttacacc gtttatatgt tttccggtat
     6121 tttgatcgtt gttccattat ttaaaatcat ttccggtcta ggtttgtatg ataccaatac
     6181 agcactgatt atcacgatgg tcgttcaaac acttcctact gcgatattca tgctaaaaag
     6241 ttattttgac acgattccga ctgatattga agaagcagca atgatcgatg gtcttagccg
     6301 gatccagatc attattcgga tcattatacc attggctatt tctggaatca tttctgtctt
     6361 cgtttattgc tttatggttt cgtggaatga ttatttattt gcttcgattt tcctgtcgac
     6421 ctccgaaaaa tttactttac caatcggttt gaacacatta ttcagtacac cagactacat
     6481 ttggggacga atgatggccg cttcattggt gaccgcttta ccagtcatca ttatgtatgc
     6541 gttatctgaa cgatttatta aaggtgattt agtcgaaggc ggcgtaaaag gataaaaaga
     6601 aaagaaagag gaaagaaaag atgaaaaaat tagttgcatt agaaccaaga atagccggat
     6661 tcaaagatta cgaagaccgt gagatcgaaa agaacgaagt acgtatccga gtaattttcg
     6721 ctactccaaa accaggaaca gaaattgtcg actttagagg attaagtcca tttatcgatg
     6781 aagaattttc agaagaatgg cgcctattct tgccaagaga agaaggaagc caacgcggtg
     6841 tggtatttgg cgaatttcaa ttaggaaata tgattgtcgg tacgatcgaa gaaacgggca
     6901 gtttggtgac agagtatcaa aaaggagagt tagtctgcac ttatggaccc attcgtgaaa
     6961 cgctgattac aaacggtgtt gataattata aactacgtaa acttgatcgg ttggatcaat
     7021 ggaaaaatgc agtatgttac gatcctgctc aattcgcttt aggtggtatc cgggaagcac
     7081 atgttcgagc aggtgatgct gtagcaatct tcggtttagg cgctatcggg caaattgcga
     7141 ttcaattagc taaaaaagca ggtgctacta ccatcgttgg gatcgatccc atagcgcagc
     7201 gtagagaatt agcagaaaaa cacggtgccg attacacgct agatcctatg aaagcagatg
     7261 tcggttttcg tctaaaggaa ttgacagata aacgtggagt agattcagtt attgaaacga
     7321 gtggcagtgc tgccgcatta caagcttctt taatagggat cggctatggc gggatcattt
     7381 cttatgttgc ctttgctaaa ccttttccag ctgggtttaa tcttggtcgc gaagcacatt
     7441 tcaataatgc aaaaatcgtc tttgctcgag catctagtga accaaaccca gattacccaa
     7501 gatggaaccg taaacgaatc gaggacacat gttgggaatt attgatgaac ggttacttgg
     7561 attgtgaaga aatcattgat ccagttgtgc catttgcaga aagcgcagaa gcttacatgc
     7621 gttatgtaga ccagcaccca gaagaaagta tcaaaatggg cgttcaagtc cgaggaggag
     7681 aacaatgaaa ttcggtacgc aagatcaagc atttttccca agagacattt tagaaaaatt
     7741 tagatttatc aaagactgtg gatttgaaga atacgaaatc gacggtaagt tgttaataga
     7801 aaatatcgac acagtcaaac aggcaatcaa agaaacaggg atcccagtag tgacagcttg
     7861 taacggctac gatgggtgga tcggtgactt tatcgaagaa cggcgtctta atggtgtacg
     7921 tcaaattgct gagattttgc gtgcactaag cgaggttgga ggcaaaggaa tcgttatccc
     7981 agcagcttgg ggaatgttca cttatcgatt accgccgatg gtttcacccc gaagcaaaga
     8041 aggagactat aaagcagtca gcgaatcact tcgttattta gataaagtag ccgaagaaac
     8101 aggtacggtt atttatttgg agcctttgaa ccgataccaa gaccatatga tcaatttgtt
     8161 agcggatgcg cgtcattaca ttgactctaa tcaattgaaa catgtgcaga tcatcgctga
     8221 tttttatcat atgaatattg aagaagatga tatttcaaaa gcattgacta cgcaaaaaga
     8281 gtatgtgggt catatccatt tagcagataa tcatcgctac caaccaggat ctggcgcaat
     8341 tgattttact cgccattttc aaacgctact tcaaaatgag tatgaaggtt atatggtata
     8401 tgaatgtcgt ttgcgaggag aaaatgaagt gaaaatttat caggaatcat tgcaatactt
     8461 gaaaaactgt ctggaaacag cgcgtcattt aaatgattta gagaaagaat agagaaagaa
     8521 taacaataag taaataagca aaggagagag aaataaatga agaaagtagc aatcataggc
     8581 gctggacaag tagcagaaaa agtacatgct gcttattatc aaaaaagatc agatgtctta
     8641 gaattggccg ctgtggttga tccagtcagc cagcgagcag aagacttttg ccgacgtaca
     8701 ggattttctc atgcgtatac atcagttaca caaatgctga atgagatttc tcccgatatc
     8761 gtgagtgtct gtacacccaa tcgctttcac ttcgaacatg tattggctgc cctcgaagct
     8821 aatgcttcag tgatgtgcga gaaaccacca gctatgacag ctgatcaagc aagaaagatg
     8881 gcagagacag ccgataaaaa cgggcagatt ttggcttatg gattccagca tcgtttttct
     8941 gacgaagcaa caatgatcaa gaatcatctt gatgttctcg gggatattta ttacgtagaa
     9001 gcgaatgcgc ttcgtcggtc tggtgttcca ggctggggaa cgtttatcga caaagaactt
     9061 caaggaggcg ggcctttgat cgattatggc attcacatgc ttgatacagc gatgtatatt
     9121 actggatttc ctaaagtaaa aagcgttcgg gcgtacagct tccaaaaaat tggtacgaaa
     9181 aaaaattcag ggaaattcgg gaaatgggat ccaagcaaat tcacagtaga agatgcaatt
     9241 tttggcacac tggaattaga aaacggcaag atcatccaac tgaatacttc gtttgctttg
     9301 aatatcagag aagacaaact gaatgtggct ttttgtggcg atcaggcagg tgctaatctg
     9361 tatccaggcg aactattcga agatgtagat ggaaagctgg tttccttaca aaagaccacg
     9421 ccagttaatg acccacatga actaagtatg gctcattttg tgaatcgggc gctcggtgac
     9481 cagactcaga tgattgcaga tggctggcaa ggatatgtca tccagaatct ggtggaaaat
     9541 ttatatcgtt cggcagaaac aggagaagtt attaccttat gaaagtagaa gaaaaacagt
     9601 ttgatttaga gtatatcaat aagtatgcga gtcagatgac cattggaaat ggattttttg
     9661 gtatcagagg aagccaggag gaagactatc ggaatcaagt aaggggaatg tttgcagctg
     9721 gtgtatataa ccgaccttta ggagcagaat cagccgagtt agtgaatctc ccagatgtca
     9781 tcagatggga aataaaaatc gacggtgaac atttttctct tgagacgatg aaagttcttg
     9841 catacgagcg ggcactagat ttagaaatgg gagagcttaa acgctatgcg ctcattgaaa
     9901 ctgctcaagg aaaaaaagtg gaagtcatta cttgtcgtgt tgctgatcaa aataatctcc
     9961 atcaggcagc cctgaaagtg gtcatcaagc cgttgaccga agactgccaa gtagatgtga
    10021 aaacgggtat cgatggacag caaaccaatt ttggtacaca gcagttggtt gaaaaagaac
    10081 tgcgagtatt tggtgacgat ttgatcagtg catcctacca gacaacagaa agtaaaattg
    10141 atattgggat tgcagctaaa ttcaataaag caggtgtatt ccaagcaaaa aatcgccgga
    10201 tcaccgcttc cttttcagaa aagatcagaa aaaatacgtc ctttacgctg gagaagatca
    10261 tcgtgctctc tacctctctt cagcaaacag acccgtttaa gaaaagtatt gcccaagccc
    10321 agaaaagcgt tagttataca gaaattttag agcagtcgat gtcttattgg acagattttt
    10381 ggcatcagca tcggataacg atccaaggcg attcttcttt cgatcagtta gcgatggact
    10441 tcgctgctta ccatttacag attatgactc cccgcaatga tccaagtgtt agtgtagggg
    10501 cgaagggact aactggcgaa ggttataaag ggcatgtttt ctgggatacg gaaattttta
    10561 tcctgccttt ctttttatat cagtttcctg aagtagcaaa gaatttgctg ctgtaccgtt
    10621 atgatcgatt agaaggcgca cggcaaaaag caagagatta tcactatcaa ggtgcacttt
    10681 tcccatggga aagcgcacac actggtcagg aagagacacc caaatttgct gcattgaata
    10741 tcaaaaccgg aacaagacaa gaagttgctt cagctgccgc agaacatcat atcagtgcag
    10801 accttgctta tgccgttcaa gagtatgtcc aagcaacaga ggatcaatcg tttttagaaa
    10861 caaagggaaa aacgctcatt catgaaacag cagctttttg gctgagcaga gctgtaaaag
    10921 tcgatgatca tttagagata cacagagtca ttggtccaga tgaatacaca gaatatatcg
    10981 acaataatgc atatacgaac tatctggcgc attataatgt ggccttagct gttaaatatc
    11041 aagctggcga tccgcttttt ttgaaagaat gtcaaaattt tttagataag ttatatttgc
    11101 cagtgcctaa caaagaacag ctgattccgc aagatgacac gtatctcagt aagccagtta
    11161 ttgatttgac cacttacaaa gaacgtcctg gcagccagtc tattttactt gattatgccc
    11221 gtagcgaagt aaatgaactc caagtcagca agcaagcaga tcttgtcatg ctgttatact
    11281 tattacctga gtatttttct caaccaatcg tttctaagaa tttgcattac tatgaaaaca
    11341 aaacaatcca cgactcatcg ttgagtaaag cgattcacgc catagaggct tgccgtatcc
    11401 aagaaatgga atgggcgtat catttattcc aagaagcgtg cctgattgat ttaggaccag
    11461 atccgcattc atcagatgac ggaattcatg ctgcttcatt aggagcacta tggctagcag
    11521 ttatatttgg tttttcaggt attacagtaa cgaatggaac tctttctatt tctccaagac
    11581 ttccttcagc atgggaaaaa atttcttttc cttttatttg gaaaaatcac aggttgctat
    11641 ttgttttgac aaaaaaacaa atagtaataa aaaaagagac gaaagaaaca ttacctattt
    11701 ttgtcaatgg agaccaatac cagctggaaa aagaactgat cttggaacgg aggggaaaat
    11761 aatgcgtatt ctgattgcgc cagattcttt caaagaaagt ctgacagcct tagaggcagc
    11821 acaagccatt caatcaggtt ttcaagaagt ttttgttcag gctgaatatg atctgctacc
    11881 tataggcgat ggaggagaag gaacggtttc atcattagca gacagtttaa ggttgaagcg
    11941 gcaaacagca gtcgttacta atgcatttgg caaaaacaaa gaaatccagt acgcttcaga
    12001 cggtcaaact gcgatttttg aaatggcaga aatcgtcgga ttgaaagatg tcccactaaa
    12061 ccaacaacag ccacttaggt tatccacgcg aggagtagga gaactgatac gacaattgtt
    12121 gcaacaaggt gttcgacaca tcatcatcgg cgttggcgga agtagtacca acgatggcgg
    12181 catcggcatg gcagaaggtc taggttatgc tttttacgat caagataatc aaaaagtaga
    12241 agcactggga gaaaatctag gtaaaattgc tacagttgat aaaacgaacg tgcctattga
    12301 gctgattaaa gccaaaatca ctgtcattac tgatgttact aatcttttat gcggaaaaga
    12361 aggcgcttct tttgttttcg gaccacaaaa aggcttgaaa gaaaatgaat tagcagtggc
    12421 tgaccaagcc atgcatgatt tttatatgag atttgcccct caagtaatcg aggcatcggg
    12481 cagtagtgct ggtggtggaa tgggtgcagg actgttagca tttgcttcag ggaaaatagt
    12541 ctctggcatc gactacgtat tggatttact ggagttcgat aaacgggtac agcaagcaga
    12601 cctcgtaatc gtaggcgaag gacgtctaga tggacaaagt ctaaaaggaa aagcgccagt
    12661 tgggattgct agaagagtac cagagggcgt tcctgtgata gctatttgcg gaagtgttgg
    12721 taaaggaagc gaaaaagcag cagatgttgg catttcggcc gtttttccga tccttcctgc
    12781 agctgtgcct ttacaagaag ctttacagca agccactgat aatcttcatc gcacagctaa
    12841 aaacatcgca tctttacttc aagtcgttca ttttcaagga ggtaatcgat gaaaatcaaa
    12901 gcagcactat tcgatttaga tggcgttctt gtagacacag ctcgttatca ttacgaagcg
    12961 tggctggttt tagctaacca gctgtccatt ccattcactg agaaagaaaa cgagcaactg
    13021 aaaggaatat ccagaaccga gtcactagag cgattgctgt catttggaaa aatggaacag
    13081 aaattctcag aaaaagagaa atcagccttt gctgagcaaa aaaacaatct atatcttcaa
    13141 gcgattcaaa aaatggatga aacatccgtt ttgcctggtg caatagctgt tcttgagtac
    13201 ttgaaaaaaa caaacatcaa gatcggattg ggatcagcaa gcaaaaatgc acgcctcatt
    13261 ttagaaaaaa cgaatttgac aagttatttt gatgtcctta ttgacggcac gcaagtatca
    13321 aaagcaaaac ctgatccaga agtttttctc aaaggagcac agcaattgaa tgtaccgcca
    13381 aatgcatgct tggtcattga agattcagaa gctggttgtc aagcagcttt ggctggtaac
    13441 atgcatgtat tgggaatagg tgaaaacata aatcttcctt ctgctgaata tgtgattcct
    13501 gatttaaccg tttttgatca agtacgttcg ttttggcatt tatcagaagc cgtatcacgt
    13561 atataatagt ttccgttttt tctgtcacaa gcaaaacgtt atcctccttt tggaaatcaa
    13621 ggtatgatga gaaaaaaggg ggattttata tgacaaaaaa agcagttttc gcaggcggat
    13681 gcttttggtg tatggtcaaa ccatttgaag agcagccagg gatcatttct gtgacatccg
    13741 gttatactgg cgggcatgta ccaaatccaa cttatgaaca agtaacaaca ggcacaacag
    13801 ggcatacaga agcagtagag atcgaatatg atccagaagt tatttcttat gatcagttgg
    13861 tggaaatcta ttggcagcaa accgatccga ctgacgcatt aggacagttc gctgatcgcg
    13921 gtgattctta tcgtccagtg atctactata atgatgaaga agaacgaaat attgcagagc
    13981 gttcaaaaca agcattgcaa gacagcgggc gattcgataa gccaatcgtg acaaaaatcg
    14041 aaccaagaag cgaattttat cctgcagaag attaccatca ggattattac aagaaaaaca
    14101 agctccatta caatctttat cgagaaggtt ctggacgtgc aggtttcctt cgtcgacatt
    14161 ggggcgtcaa gaaataagaa gccgaaaaag ctacttatgg aacaccgtat atcagaggtt
    14221 gtaaaaccag cattttgagc taatactgag ggtcagctga ttgaacacca tttagtggag
    14281 aggttgtgaa cctgtcgcct agctccaagt cacaaggatt tggtaagaga acactatata
    14341 tcagaggttg tgacaagtcg tttgtcacaa ccttttttca atacctaaga tgaaggacaa
    14401 tcagcaacgc actgctcaaa aaaggaatga aaggcagctt ttccaacttt tttttatgac
    14461 agataaagta aacagaaaaa acggatattc caaacagact agcaagaaac aagatctgaa
    14521 gcaactcttc tagagacaag ccaccactcc aagaaagcat caacagagta tcgccagctc
    14581 cgagatagga ttgaccatag cgaaggcaca caccgcctac gataaacgtt aacagcacac
    14641 tttgccattg gaagacgccg gcctgcaacc aaaaaatcca taaaattgcc caagaacaga
    14701 atagcgtgtg agaatggacc atgaagtaaa ataaatccat caacgaaaat agcaaagccg
    14761 aaagcatcca tatgaacgtc gaaaaatttc ttgcggaaga aaaagtaaag aaccaggcaa
    14821 aaagaccacc acataaggct tctgccaaca aatagtagaa gggcaaccga cacctgcaat
    14881 agcggcagcg aaatcgttgg acgatgatcg agagaatcgg aatcaattca taccaagaaa
    14941 gcgaacgaca gcatttgaca caatgagatc taggataaat aatagagtga cctaacggaa
    15001 tccgctgggc ggttaaacat aaaaatgatc cgatacagca accaatgata aaataaagta
    15061 acacgccatc tccccctttt caaaaccaag atacgcaagc ttaaactaaa tttttgtaca
    15121 tacataaaat ttgaggtgaa aaaatgaacg tttcaatcgg attagatatc ggaaccacac
    15181 aaaccaaagc agttgctttc acagatgaag cccaagaagc agcatccgct tattttcgct
    15241 accctttgat ccaagaaaca caagggatgg cagaacaaga tcaggaattg atctttcaag
    15301 gtgtctgcac cgttataaaa gaagtaatta aacagttgcc ttcagacaca atcattcaat
    15361 ttgtctcctt ctcctctgcg atgcatggcc tgatattgtt ggatgaagag aaaacaccgt
    15421 tatctcgaat gatcacttgg gcagataacc gagcagcgga cgaagcagaa aaactgaaat
    15481 ataccgtgct tggcaaagaa ttttatcggc agactgggac gccgattcat ccaatgacac
    15541 cattgaacaa aatcaagtgg ctgcaaaaca gccaacctga attattcaaa aaaactgctt
    15601 attttgtcgg tatcaaagac tatatctttt atagattgtt tggcgaactg atcagcgact
    15661 acggaacagc ttcaggaact ggatattttg atatccatga attccaatgg gcagaagaag
    15721 tgcttgacta cttgaacatt cgaatagagc aattgccgca agtctttcct tccacctatc
    15781 aagtaacagg tctaccatca aaaacagcag cagaactagg tttgccaaat gatatatcat
    15841 ttgttttggg aggggcagac gggccgcttt ctaatctagg gttaggtgct tttggtgata
    15901 cgatcgccgc tttgaccgta ggaacgagcg gagcacttcg ttttattact actcagcctc
    15961 agttccatcc agaaatggag acgttttgtt atgttctgga tcagaaacat tgggtagtgg
    16021 gaggagcgac tagcaatggt gctggcatct ttgattgggc agggcataca tttttacagg
    16081 aagtcgttca atctgctaaa gaaaaaggag aaaatccata tgatgcactg ctttcggcta
    16141 tcgcttttgt gccagcgggt gccaatggtc tgatttttca tccctattta cttggtgagc
    16201 gagcaccgct ttgggacgca gatgcgtttg gcagttttat cggtttacag cggcaacata
    16261 cagaaaaaga aatgatgaag gcgattctag aagggatctg tctcaacctt tatcggattt
    16321 taaaaggagt ttgccaggaa aatcagctca cagagatccg tgctacaggc gggtttgctc
    16381 aatctccgat ttttcgtcag atgatggctg atgtactggg ttacccgctt gttttcccta
    16441 ctgtcacaga agcatccgca ttaggagctg tgattttagg ctggagaagc atcggaaaga
    16501 tccaaagtat cagtgaagca caggaattga tagaattgaa agagcaagtt ggagtacaag
    16561 aaactgatca taaaaaatat cagaaaatct atccattatt tgtttcgaca caaaagcatt
    16621 tagcacagac ttatcagcaa tttgctcaac tgcgagaaga attagctgat tctgaaaaat
    16681 gattggcttt acggataaaa aagcgaactg aaaatcgttc tcaggtaaat gattttgttg
    16741 tacttttcat tgaaagtcta ctattatttt aagtgtaaga aaatgttagg aggcaagaaa
    16801 gatgaaattc caacaaacca aagaagtatt aaatcaatta gttgcagatc taagccaaat
    16861 gtctgttgtt gttcaccaaa cacattggta catgagagga ccaggtttcc ttacattgca
    16921 cccaatgatg gatgagttca tggatgattt gaacgctcag ttagatgaag tatccgaacg
    16981 cttgatcaca ttagacggtt caccttattc tacattgcgt gaatttgcag ataacacaaa
    17041 aatccctgat gaaattggcc gctgggatcg tactattcca gaacgtttgg aaacacttgt
    17101 tgcaggctat cgttatctag cagatcttta ccaaaaagga atcgaagttt ctggagaaga
    17161 aggcgacgat ccaacacaag atatctttat cgaattcaaa acagccacag aaaaacgtat
    17221 ctggatgatc caagctcatt tgggcaaagc gccagagatc gacgcgtaaa aactgttgtt
    17281 gaacttgacc gaaaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    17341 acgtcaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    17401 tgaacaccgt ttaccaagag gttgtaagcc tgccgttcag ctccgagtca caagaaacaa
    17461 ttttaaaaaa cagcttctta tgttttatgg atattgtgac ttgtgaccga ggagttggca
    17521 ggagaacacc gtttattcaa aataaggggt tgtgacaaga cttttgtcac agcctctttt
    17581 tgtttttcat tttgacagga aacgaccctt ttatataaaa gaaagaaaag aagtaaagtt
    17641 tcatataaat ttttcaatca agcagaaaaa cgttgacatt gactagatta gcgggtaata
    17701 tgttaaatgg tattgtttgc cccacaaaga gccccggaaa ctcgaagtgt atgtcaaaca
    17761 tcagaactta aattggagga aaataacgtg cgtacaacat atatggccaa accaggcgaa
    17821 gtagaacgta aatggtatgt agtagacgca actgatgttc cattgggacg tctttcaaca
    17881 gttgttgcat ctgtactacg tggtaaaaat aaaccaactt tcacacctca tttggatact
    17941 ggcgatttcg tcatcgtcat caatgcagac aaagtgaaat taacaggtaa aaaagcgact
    18001 gacaaaattt attaccgtca cagcaactac ccaggaggat tgaaatcaat cactgctggc
    18061 gaattgcgtg ctaaaaattc tcgtcgtttg atcgaaactt ctgtaaaagg tatgcttcct
    18121 aaaaacactt taggccgcaa acaatttaca aaattgaacg tatacggtgg agcagaacat
    18181 ccacatgcag cacaacaacc agaagtttta gacatcacaa acttaatcta ataggaggga
    18241 atttcattgg cacaagttca atatatcggc acaggccgtc gtaaaaacgc agttgctcgc
    18301 gtacgtctag taccaggaac tggcaaaatt actgtaaata aaaaagatgt tgaagaatac
    18361 attccacatg ctgacttgcg tgaagtaatc aatcaacctt tcgctgttac tgaaacaaaa
    18421 ggcgcatatg acgtatttgt taacgtaaac ggcggaggat acgctggaca atcaggagct
    18481 atccgtcatg gtatcgctcg tgcattgcta gaagttgatc ctgacttccg tttagctctt
    18541 aaacgtgctg gcttacttac tcgtgacgca cgtatggtcg aacgtaaaaa accaggtctc
    18601 aaaaaggctc gtaaagcatc tcaattctct aaacgttaat attatttata tatcaatgtt
    18661 tcaaggcact tttcaattgt ttgaaaagtg tctttttctt gtttaagaac acatttaaga
    18721 acacatacta atcacattta ttcaatcctc tctgaaaggc ttcaaaaaca gttttatcta
    18781 ctacatctgg tgtatttacg tatattctcg ttgtagaaat atctgagtgt gttaaagcac
    18841 tagatatttg attcatattt gctccgcctt gtctagctat agttgcatag gtatgacgaa
    18901 gcttatgtgg agataaatga gccaattcag gatgtcgtct tttaattgaa ttgattcgat
    18961 agtttaggta atcagcgtga acagggcaat ttacttctcc ggatggttta gtataggtaa
    19021 agagatactg atctggtgtt tgattaattt ttaatctcaa caattgctca gcttgcactg
    19081 atttccattt gcttagtaat gccattacaa tttcgggtac ttgaattacg gtatcctttc
    19141 gaccttttgt aaatttcttt ttttgatatt tatccaaagc atgtttaagg cgaaccgttt
    19201 gtttctcaaa gtcgatatat ttccattgca aagcatacgt ctcacttttt ctatcaccaa
    19261 gatatagggt aagcatgaaa agagtatagt catgaagagt aagcaagtga ttattcaagt
    19321 cagtgtttac tgcttctatc caagcaataa gctcttctga cgataatgct tgattacctt
    19381 cccgtatcct tctttcttct aaagcaattt ttttaggtgc agtgatacat tgaataattt
    19441 tggtagttcg gttaaattca atataattta gtatttctgc aatatcaaac atagaacgga
    19501 catatccttt gataattttt atatttgcgt attctttcga tttttttgtt aattcatctg
    19561 atattatctc agtatttgaa tttagatagt tcattgcata ctcaccaaac attggaagga
    19621 tgtgcaatcg gaatatatct ttagtattac taattgttac gtaacttgga gctctttttg
    19681 atctagtagt ctgacctagt tcatatctag gaagccattt tgattcatag aattccttaa
    19741 attttatttt tccttttaat tctagtgaat ttgcattctc ctctcttaaa actgtttcaa
    19801 ttttcctttt cattgcattt tcggctttaa tagccaattg cctagttgga aatacttttt
    19861 gatacttttc tgatgtaatt ccagtgattt ctctgaattt ttttggatac ctcagattag
    19921 ttttatattt cccatttgaa agttttgtag gcaaaattcc tcactccctt aaaaagtaag
    19981 gcagtatatt tcgctattct tagtatatgc ggcgagaaga aattttcaaa ttgttttaaa
    20041 tgtattcata aaatctttga tatcctctat gtcaaagcga actataccgt caattctgat
    20101 tactggaaaa ttatatatac gaatccactt atctaaagta ttattatcaa ttcctagaaa
    20161 ctcacaggtt tgttttttat tcaagtagac tctcaatttt ataaaatcat ctcctaaaaa
    20221 agattagaat ttttgaattg ctttgggtaa gcacatagga atttcacctc tccccagttt
    20281 acggcgagcc ttacggaagt atcattattt gctgctattg tcatggcatt ttgttttccc
    20341 agaacaaaaa gaaatctcta agtatttgtc gctaatcagg tcatggcgta cttgagtttc
    20401 tagctcgaat agcagggatc gttattcaaa aattgtattt aattgtcaat gatcaaaaat
    20461 aggttttttc ctattcacta ttaataacga taaacatgta taaaaagaca cactacgaaa
    20521 aaagttaaat ggagtttact gattttcata tttgtatatg gaacagacat agtcttgaat
    20581 aatttttata aatgttttga tgcttatcaa cttattcaac ccgctatcca ctttaagtat
    20641 aatttttcca tacgccaata aagtagtttt aggagcatgt tctcatttga gaacgctcct
    20701 tttttatata ttttgaaagc tacttactat acgcgtagtt tcaaatagtt ttgtcattct
    20761 aatcttcagc aacagaaaaa aatctaaatt ataaaattta gggttgacta aaatataatt
    20821 tttgtatacc atatgatcgt gataatcatt atcatttaga aggagaaagt tatgttagaa
    20881 gtgaaaaagc ttaatgtttg ttataacgat tttttagcat tgaacgagat ttctttaaaa
    20941 atacaagaag gtgctttaac aggtattatt ggtccaaatg gtgctggaaa atctacattg
    21001 cttaaagcaa tgttaaatat tatccctcat cagggagagg tctccattaa taatgaagat
    21061 atcaataaaa aactggtgaa gattgcatat gtagagcaaa aagctgatat tgattttact
    21121 tttccaataa aagtaaaaga atgtgtatct atgggaacat atgccggaat gaaagttttt
    21181 caaagaatta aaaatgcaga atggcaaaga gtttcaaagg ctttggaaaa ggtcgatatg
    21241 ggaaaatatt ctaatcatca aataggagaa ttatctggag gacaatttca acgtgtactt
    21301 ttagcaagat gtcttgctca aaatgcggat ttcatatttt tagatgaacc atttgtaggg
    21361 atagacttag ttagtgaacg aataattatg gatacgttaa aagaattgaa acatcaggga
    21421 aaaacagtgc taattgtgca tcatgattta agcaaggtaa agaagtattt tgataatatt
    21481 attcttttga atcgaaatct aattgctcac ggaagtgtag aatctgtatt taatgaagag
    21541 aatttaaaaa aagcctatgg agatactata tttatt
//
LOCUS       DACEKH010000042        21504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEKH010000042 DACEKH010000000
VERSION     DACEKH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.043"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..403
                     /locus_tag="IW662_001473"
     CDS             5..403
                     /locus_tag="IW662_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0866304.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IW662_001474"
     CDS             387..1082
                     /locus_tag="IW662_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ0866305.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1666..2553)
                     /locus_tag="IW662_001475"
     CDS             complement(1666..2553)
                     /locus_tag="IW662_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ0866306.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2738..3577
                     /locus_tag="IW662_001476"
     CDS             2738..3577
                     /locus_tag="IW662_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0866307.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3773..4747
                     /gene="sgrA"
                     /locus_tag="IW662_001477"
     CDS             3773..4747
                     /gene="sgrA"
                     /locus_tag="IW662_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0866308.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5045..5401
                     /locus_tag="IW662_001478"
     CDS             5045..5401
                     /locus_tag="IW662_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866309.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5404..6561
                     /locus_tag="IW662_001479"
     CDS             5404..6561
                     /locus_tag="IW662_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866310.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6587..7027)
                     /locus_tag="IW662_001480"
     CDS             complement(6587..7027)
                     /locus_tag="IW662_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0866311.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7187..7693
                     /locus_tag="IW662_001481"
     CDS             7187..7693
                     /locus_tag="IW662_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0866312.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7861..9774
                     /locus_tag="IW662_001482"
     CDS             7861..9774
                     /locus_tag="IW662_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0866313.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9779..10780
                     /locus_tag="IW662_001483"
     CDS             9779..10780
                     /locus_tag="IW662_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0866314.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10982..11188)
                     /locus_tag="IW662_001484"
     CDS             complement(10982..11188)
                     /locus_tag="IW662_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ0866315.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11834..12682)
                     /locus_tag="IW662_001485"
     CDS             complement(11834..12682)
                     /locus_tag="IW662_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0866316.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12846..13748
                     /gene="gnd"
                     /locus_tag="IW662_001486"
     CDS             12846..13748
                     /gene="gnd"
                     /locus_tag="IW662_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ0866317.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13750..14751
                     /locus_tag="IW662_001487"
     CDS             13750..14751
                     /locus_tag="IW662_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0866318.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14748..15032
                     /locus_tag="IW662_001488"
     CDS             14748..15032
                     /locus_tag="IW662_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0866319.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15065..15862
                     /locus_tag="IW662_001489"
     CDS             15065..15862
                     /locus_tag="IW662_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0866320.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15876..16709
                     /locus_tag="IW662_001490"
     CDS             15876..16709
                     /locus_tag="IW662_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0866321.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16727..17584
                     /locus_tag="IW662_001491"
     CDS             16727..17584
                     /locus_tag="IW662_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0866322.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17606..18115
                     /locus_tag="IW662_001492"
     CDS             17606..18115
                     /locus_tag="IW662_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0866323.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18152..18553
                     /locus_tag="IW662_001493"
     CDS             18152..18553
                     /locus_tag="IW662_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866324.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18665..19018
                     /locus_tag="IW662_001494"
     CDS             18665..19018
                     /locus_tag="IW662_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866325.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19052..19495
                     /locus_tag="IW662_001495"
     CDS             19052..19495
                     /locus_tag="IW662_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0866326.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19512..20597
                     /gene="uxuA"
                     /locus_tag="IW662_001496"
     CDS             19512..20597
                     /gene="uxuA"
                     /locus_tag="IW662_001496"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0866327.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20590..21303
                     /locus_tag="IW662_001497"
     CDS             20590..21303
                     /locus_tag="IW662_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0866328.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7400 a         3302 c         4491 g         6311 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta cttatttctc atttaaacga
     1201 tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataaa
     1261 tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc tctagtataa
     1321 cagaataaga ggaacaagtc cagaagggat atttttagtc ttttttaaaa taacttttca
     1381 gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa aaaataggga
     1441 agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa aaaacttttt
     1501 taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca aataaaaaaa
     1561 ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga atttccaaaa
     1621 atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt tgaatatatt
     1681 ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac ggattgtttt
     1741 atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta gctcagattt
     1801 gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta gataatagct
     1861 ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc cgttttttag
     1921 tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat caaaattcat
     1981 ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa gcttggagaa
     2041 attttcccct tcatttgctt ctttttttgc tttatctgct tcttctttag tcgaaaatag
     2101 aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta gatctttatc
     2161 tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt taaagagttt
     2221 cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt ttggaccgag
     2281 ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct cttttccgta
     2341 tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat tatttgattt
     2401 aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa acgttgcaat
     2461 cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg aaaggcacat
     2521 aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa cagtctaaca
     2581 gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt cttagttcgt
     2641 aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga caagaaagaa
     2701 aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta ttcggttgat
     2761 tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca ataatgcact
     2821 tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta cggtcggaca
     2881 tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa aaagaataga
     2941 agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag gtctcgttgt
     3001 tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg cgtatcggtt
     3061 agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat ttattgcagt
     3121 gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc ttagatttgg
     3181 tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg gattgaatcc
     3241 taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt tcagcgcact
     3301 tatctacagt acaggaagtt tatttacggc tattggtgct catattactt ggaattggac
     3361 acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt ttttctccac
     3421 tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg aagcatctat
     3481 atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg catataaaac
     3541 aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag caaggctgat
     3601 atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca aattcaagcg
     3661 aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca tcattttcat
     3721 tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag aaatgaagaa
     3781 aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa tgattggaat
     3841 cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga aaactgatcg
     3901 gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa acaaaagcgt
     3961 gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg gaagttcaga
     4021 aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta ttgagtatac
     4081 aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata aagttgattt
     4141 gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa gcagttggac
     4201 agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta cagaaaacac
     4261 gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa acacgagcag
     4321 cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga gcagcgaaag
     4381 cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg agagtagcac
     4441 aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg atttggtacc
     4501 tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa gtgtgctaaa
     4561 acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag aaacagataa
     4621 tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa ttacatggat
     4681 gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct tccatcgagt
     4741 gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt tttgagtgaa
     4801 tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag atctattttt
     4861 gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa ataagagcaa
     4921 gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt aacgaatcta
     4981 tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa aaggagtatg
     5041 tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat ttttgaacta
     5101 ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag atctaaacta
     5161 caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg aaaggtatcc
     5221 agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt tttcttctca
     5281 gtttttactg agtaaagttt attctatact tttagaggga acgattggtt atattttact
     5341 ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa aaattatata
     5401 agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa aaactatcat
     5461 ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg tatccgatac
     5521 ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat attatcaagt
     5581 ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata tacgcgtagt
     5641 atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat gaaacaaggc
     5701 cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt gataacaaaa
     5761 gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg gtcgatgaat
     5821 cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat gaacacctat
     5881 ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg ctggattgaa
     5941 tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac ttcagaaaaa
     6001 aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt tgggacaaaa
     6061 aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca tatatttcca
     6121 gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat tccagagctt
     6181 aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt agtcagggat
     6241 atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta tggtaaaaca
     6301 caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat aaaatatagt
     6361 gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga caaaaaggca
     6421 tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa aaatgattgg
     6481 gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc catattggaa
     6541 aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa gacattgcaa
     6601 aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg ttgagctctt
     6661 gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat ccagtcaaaa
     6721 gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat gtacgaatct
     6781 caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt ttataactaa
     6841 tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt aagtggataa
     6901 attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt atatcatgga
     6961 cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta tacaatttaa
     7021 tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa gaaatagtcg
     7081 aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg gaactagcta
     7141 gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt caaacacagc
     7201 cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt cgccgataca
     7261 cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg aaacgattgg
     7321 gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa caaaaccatg
     7381 ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa aaacccatgc
     7441 gaattcattt tataagacta atttgagtgt tttattaact gaattggact gtcattcgat
     7501 cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt ttgctcatgg
     7561 attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata atccttttat
     7621 gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat ttctgacatt
     7681 tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg gtttatccat
     7741 tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg ataaaccatt
     7801 tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac ataaaaagga
     7861 gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt atttgctgta
     7921 agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga atcggaatct
     7981 cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc gagtacttca
     8041 caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac agatcaagaa
     8101 acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa aatgtcaaac
     8161 gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa tgatgatatc
     8221 ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa acagatgaca
     8281 gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt attagtcact
     8341 gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct gattgctact
     8401 tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat gcttggaggt
     8461 acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac aaaacaactt
     8521 catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc atattatgtc
     8581 gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa ttggaataac
     8641 gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt attatttgtt
     8701 gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca atatgagtca
     8761 gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca aaagatttta
     8821 ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa agatattcct
     8881 gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga acaactacgt
     8941 tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa tcagttagac
     9001 atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca atactatttt
     9061 gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat gtcagattca
     9121 ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc taacgcgcaa
     9181 aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg agcagggcat
     9241 ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga ctttttgaaa
     9301 gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa aagtctactc
     9361 aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat gacagagcaa
     9421 aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg gatttttaat
     9481 gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc accaaaaaag
     9541 caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga ggatcaatca
     9601 agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac agatgcaaaa
     9661 aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta ttcaggtgga
     9721 tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg gtaaatgaat
     9781 gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta ttttagtcac
     9841 tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta gaaaacaatt
     9901 tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc aaaaaatcga
     9961 acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata ataatatgga
    10021 tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga tgcatggagg
    10081 cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc tatgttcaga
    10141 cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa aacatcctac
    10201 accagttatc caattgaacg aagccattca agcagctaaa aaagagataa aagatcaagc
    10261 agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta gtggagaata
    10321 tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc cgactttgga
    10381 gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt ctgcacttac
    10441 tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg cttactttga
    10501 taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca gacatgcgga
    10561 tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta cgaaacaaat
    10621 ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag tagattatgc
    10681 ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata aacaagcgca
    10741 agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga tgcaaaagag
    10801 gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac aagcttttca
    10861 tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg gcacctttta
    10921 ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta aattttataa
    10981 gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct gcggaggcaa
    11041 gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat attccgttgt
    11101 tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca ctagcgaaaa
    11161 aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata attgtatgat
    11221 aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa aatgatcatc
    11281 tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc aatcagatag
    11341 gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa tataatagaa
    11401 aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct aaactttttt
    11461 attttacata acaatcatat caactgtgaa ataaaattac aaatgataac ctaaaatgag
    11521 gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt taatttttta
    11581 gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact actgtatact
    11641 aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct tctccaaaga
    11701 tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc ttgcagaacc
    11761 aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat ttttcgcttt
    11821 ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta gcgatagaac
    11881 gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc aaaatgctta
    11941 gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca gaaatagaaa
    12001 taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta ataacgattg
    12061 tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt gattgccctg
    12121 tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc atcaattgaa
    12181 tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta tgataagcgt
    12241 cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta gattcattga
    12301 taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg tcatttaaag
    12361 ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct gttttctgaa
    12421 tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg gccattttaa
    12481 aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa
    12541 tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt ggattttcta
    12601 aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt gttttgattt
    12661 gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt atcatttttt
    12721 ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca aaaaatattt
    12781 ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga aatggaggcg
    12841 ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta gctttaaact
    12901 taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct gttagtcagg
    12961 caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct acttttgagc
    13021 agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac atgttaagaa
    13081 aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac tcttattata
    13141 aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac ttggattgtg
    13201 ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt ggaggagata
    13261 aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa agaggatatc
    13321 tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat ggaatcgaat
    13381 atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt
    13441 ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc ggctggctga
    13501 tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat cggggaatcg
    13561 tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg gctgttccag
    13621 ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa gatagttttt
    13681 cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg
    13741 gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt cggaagagtc
    13801 tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt tactgttgat
    13861 caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat cattattgca
    13921 agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact gctattgatt
    13981 tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga acaccagaca
    14041 atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa taatataact
    14101 aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa ggacgatcga
    14161 tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt tggtgtgatt
    14221 ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt tcgctgtgat
    14281 gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa
    14341 gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc gaatttgaca
    14401 gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga aaatgtttat
    14461 ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa tggtctgaaa
    14521 agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg acgtcaagat
    14581 catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc ctacacaagg
    14641 gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac
    14701 cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg atgaagaaaa
    14761 tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa gaaggtggta
    14821 tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg gtcacccacg
    14881 gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac gaaacgattc
    14941 aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga cgaatagcta
    15001 caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa aactgaccga
    15061 tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc ctactttagc
    15121 tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg gacaagcaga
    15181 agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc cccaattagt
    15241 gattgattcc cctgttccat atttcattgc cgggttagca gatacactag caaaatggta
    15301 tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt tgaagctagc
    15361 acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta aagcaatcca
    15421 agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga ttatctttgc
    15481 tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg cagcacatgc
    15541 tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac atggagaaaa
    15601 agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa aaatagacga
    15661 gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa tgggattatt
    15721 tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga aagagaaagt
    15781 gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga tggaactgga
    15841 aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt gtagtacaaa
    15901 gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca
    15961 cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg atcatggggg
    16021 aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg ggggtttttt
    16081 ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca gtaccagtcg
    16141 cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca gtcggattgc
    16201 tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt aaatatacag
    16261 acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt ctttatccaa
    16321 cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat ttaggggcgc
    16381 cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac attcttgaag
    16441 tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa
    16501 aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg aaatctttag
    16561 atattacgat ggttacttat gccatttttg ggattattat cgctttgatc tttgtccagt
    16621 cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca tcttttgatg
    16681 acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg ctgaaataat
    16741 ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct
    16801 gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg cgccatcact
    16861 actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact ctttaaaagc
    16921 agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg gcggtgtgat
    16981 tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg aagaaaaaga
    17041 gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg gggcactagc
    17101 aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg caatcgcggt
    17161 accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct gctttgggtt
    17221 gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg gccgtaatgc
    17281 tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat cagttttagg
    17341 gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt
    17401 tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg tgccagggat
    17461 attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag gattgaaagt
    17521 cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa tcggtatttt
    17581 ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg
    17641 tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga attccatttt
    17701 tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca aaaatgcagg
    17761 aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat tctattggga
    17821 tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc aaacgattta
    17881 cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga ttgctcaaaa
    17941 aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat cagatgctga
    18001 aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc aagccactcc
    18061 atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt cataaacaaa
    18121 tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg
    18181 ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt
    18241 tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag tcattgctta
    18301 cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa aagcagagaa
    18361 agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt tcatgtttga
    18421 ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca ttttgttgtt
    18481 cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg tattcgatct
    18541 gctatccgta taaacaggta tcactgtact agcatttggg agctactttt tcttattggg
    18601 aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt catccatatt
    18661 aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg cgtttgttga
    18721 tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga aaaaagaggc
    18781 tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt cgggaaatta
    18841 tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa
    18901 ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca aaacagattt
    18961 acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt ttcgataagt
    19021 aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg ttacacatgg
    19081 taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat cgagccatat
    19141 tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga aggtcaaaga
    19201 aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc taattagcgc
    19261 aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa agaaagcagc
    19321 cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca atcatcagat
    19381 gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa aagataattt
    19441 aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt tttagaagga
    19501 aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat tcgatcccat
    19561 taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg ctgactaaaa
    19621 tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct gttgagagcc
    19681 aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc aaggcaggga
    19741 cagatgaacg agattactat attgataact atatacgaac aatcaaaaac ttgtctcgat
    19801 gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg aaaactgatt
    19861 tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa atcgtcgatc
    19921 agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga ttcaaattgc
    19981 cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa
    20041 tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg attccagtgt
    20101 gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg gaactttttg
    20161 gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat gcagtagatt
    20221 ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt
    20281 tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc agaaacgtgg
    20341 agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg
    20401 atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct atacgccctg
    20461 atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg
    20521 caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa aaaccactgg
    20581 gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct tttattagaa
    20641 actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga ttatgaaatg
    20701 cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag agaacaaatg
    20761 ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat caatcaatat
    20821 aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc agaagaaatt
    20881 ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa tacacgggta
    20941 aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca aatcatttca
    21001 ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa
    21061 atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg gatcgaagca
    21121 gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg gttgcccgat
    21181 cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa taaagcagac
    21241 tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc
    21301 taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac tgagggaaca
    21361 gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt gaacaattta
    21421 gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa gagaagaacg
    21481 agaatcgtcc atatgagggt ttcc
//
LOCUS       DACEKH010000043        21203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEKH010000043 DACEKH010000000
VERSION     DACEKH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.044"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IW662_001498"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IW662_001498"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ0866329.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IW662_001499"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IW662_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ0866330.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IW662_001500"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IW662_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ0866331.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5847)
                     /gene="hflB"
                     /locus_tag="IW662_001501"
     CDS             complement(3742..5847)
                     /gene="hflB"
                     /locus_tag="IW662_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ0866332.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGARI
                     PAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETAKK
                     NAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATNRS
                     DVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGFAG
                     ADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYHEA
                     GHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGGRT
                     AEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQTK
                     AYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKSLF
                     EHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEENKA
                     EHDSDSQQSEDNHSDDQYK"
     gene            complement(5937..6482)
                     /gene="hpt"
                     /locus_tag="IW662_001502"
     CDS             complement(5937..6482)
                     /gene="hpt"
                     /locus_tag="IW662_001502"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ0866333.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6482..7927)
                     /gene="tilS"
                     /locus_tag="IW662_001503"
     CDS             complement(6482..7927)
                     /gene="tilS"
                     /locus_tag="IW662_001503"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ0866334.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8047..8517)
                     /locus_tag="IW662_001504"
     CDS             complement(8047..8517)
                     /locus_tag="IW662_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ0866335.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8563..8988)
                     /locus_tag="IW662_001505"
     CDS             complement(8563..8988)
                     /locus_tag="IW662_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ0866336.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9055..9324)
                     /locus_tag="IW662_001506"
     CDS             complement(9055..9324)
                     /locus_tag="IW662_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ0866337.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9335..10930)
                     /locus_tag="IW662_001507"
     CDS             complement(9335..10930)
                     /locus_tag="IW662_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0866338.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10939..14460)
                     /gene="mfd"
                     /locus_tag="IW662_001508"
     CDS             complement(10939..14460)
                     /gene="mfd"
                     /locus_tag="IW662_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ0866339.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14477..15037)
                     /locus_tag="IW662_001509"
     CDS             complement(14477..15037)
                     /locus_tag="IW662_001509"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ0866340.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15390..16364
                     /locus_tag="IW662_001510"
     CDS             15390..16364
                     /locus_tag="IW662_001510"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0866341.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16534..17934
                     /locus_tag="IW662_001511"
     CDS             16534..17934
                     /locus_tag="IW662_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ0866342.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18012..18734)
                     /locus_tag="IW662_001512"
     CDS             complement(18012..18734)
                     /locus_tag="IW662_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0866343.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18813..19319)
                     /locus_tag="IW662_001513"
     CDS             complement(18813..19319)
                     /locus_tag="IW662_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0866344.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19442..>21203
                     /locus_tag="IW662_001514"
     CDS             19442..>21203
                     /locus_tag="IW662_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ0866345.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTED"
BASE COUNT         5891 a         4649 c         3826 g         6837 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc gccgccttgt tgtcccatca tcatgtaaaa
     5401 gaaaaagatg atgattacga ttggcaagaa gctaagcaat agggaaaccc atataccgct
     5461 gcttgattct tcttttactg ttgtagcaac tttgttttct tttgccagtt ctgttacttg
     5521 gctcaatgtt gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag tcgtatctgt
     5581 tgaacctaag atagctaacc caccgctgtt agcgacggtc tgctgttctt tgtattcacc
     5641 agtgatctta tatacaccat ttgcaggctg cacttcaaat tctttgatct tgcctgcttg
     5701 tagttgttct gtgaattttg agtactcgat atctgacgat cgctggccgt tattaccaaa
     5761 aatgaaataa acgaccatta ccatcgctaa gattacgagg acataataca aggcattttt
     5821 catcatgccg ttgttctttt tgttcatagt tgccctccct aagcgtcaat ttaatttaac
     5881 agttttttat caatcctgac cattctattg taccataatt taattgagat acagcattaa
     5941 tttgattgat aaacactagg tttcaataca ccgatgtacg gaaggttacg atatgcttct
     6001 gcataatcca agccataacc gaccacaaat tcatttggta cgttgaagcc aacataatct
     6061 gcttcgatat ttactactct tccttcaggt ttgtcaagta gtgtaacaat tttgactgat
     6121 tttgctttgc ggtatttgaa aagatccact aaataagcaa gtgttcttcc gctgtcaatg
     6181 atatcttcaa cgatcaacag atcgcgacct tctacattcg tatctaagtc tttgacgatt
     6241 tttacttccc ctgacgagac tgttgcattc ccgtagcttg aaacatccat gaaatccaat
     6301 tccaaatacg tatcgataga acgaacgata tctgccatga atggtaccgc tccttttaag
     6361 acacctacta ctaggggatt ggtgttttgg taaatttctg ttaactcttt gcctagttct
     6421 ttgcaacgaa cttgtatctc ttcttgtgag atcaaaattc tctcaatatc cttttccaac
     6481 atcaggttct ccttccaatt gcttcttttt gatatttgta aagaagtatg tagtgtattt
     6541 tatcagtttc ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag ctatatatat
     6601 ttcttttggc atctgtaatg atccaagcat gctgcctttg tgaattaggt actttctgat
     6661 cgataaaata tctagatact ttttttgtga gattctctgt taatttaatg cggtctcctg
     6721 cttttctttt ttcgacatac agttgcacat cagcaggaat ccaaatgtca tgagaaaata
     6781 gagaccatga atctgttgct ttgatgtccc ctgcattttc aggtttgacc agaccaatcc
     6841 attctgtttc attcaaatat attccttgtt ctggaatcag caacaagcgc tggtcttctg
     6901 tctggctagt tgcttgtttt tcttttttag agagtacaac aaaatgatac tctcttttca
     6961 gtatccaatt attgccaata ctgagttgcc attggctttt tttctgttgc cactggtcta
     7021 aaatcgactc catttgttgt tctttcaaaa taattcctgt ttttgagaat gctttgtcga
     7081 aaaaggagta aaaaaaatag tatcgttcag catctgtcat ttcttcaatc atttctgcag
     7141 aaaatcgaaa ttgttcctcc cactgttcga cttccttttc gatcaactgt cccatatatt
     7201 tttgaatgac ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa tgtctcaacg
     7261 cctgtgtatt ttcttgtttc aattgaggaa ctaccaaatg acgaagccga tttctctgga
     7321 tatctaacaa ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc gctgcataag
     7381 tatataggtc ttctttgcta aaactcagca acggacgaat cagtctgccc gtagcaaatg
     7441 gttgtacttc tttgattcca gaaaaagtat ttaactgtcc cccacgaacg attttcatta
     7501 acaccgtctc gatttgatca tctccgtgat gagcagtcat caacgtgtca tattgtcctt
     7561 cttccatcac gcgagtgaat tcttgataac gaaagttacg agcttctgtt tctatggcgg
     7621 atttggctgg atcttcccaa atacttacat ataaagggag attcttctct tcacaatatg
     7681 agcgaagata ggcagcttct tctgctgatt cttctcttaa ttggtgattg atgtggacga
     7741 tacctaaaga aaaaccgatt tggcgttgca cttgtaaaag taaatggagc aaaaccatcg
     7801 aatcaacacc acctgatacc gctgctaaaa ttctcgatcc ttgttgccag aatccttgtt
     7861 cattcccgtg ccgaataaat gactgctgta atagcggtgc ttttttcccg cctttttcaa
     7921 cgttcatcct ttccacaccc tttcttccta aaaaaaagag agcgggaaaa cgtctaaaac
     7981 gacaattaac ctgccctctg ttgatactat gacccaaata gtcgcactat cattatttgc
     8041 ggctttttag ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt ttcgcttcaa
     8101 tgaagataag cggtcatcgc tatctttcaa aaatgaactc atcaatgaat cgaagtcttc
     8161 tttttgagct gaaggttgac tgcgagaaaa agattttttc tgtgctggac gatttcggtt
     8221 gtcacgggga tctttttgtt gatatttttt aggacggctt tcttctccag attcagcagg
     8281 ttgttcctgc gctttccgga tagatagtcc gatttttcca tcatcattta ctgaaataac
     8341 tttaactgtt acttcatctc ctacacttaa aacatcatgg atgtctttta catagccatt
     8401 tgacacttcg ctaatatgaa ctagacccgt ttttccagca cctagatcaa taaacgcacc
     8461 aaaatttgtg attcctgata cttttccttg cagttttgct cctacctcga ttgacataaa
     8521 aaaaatgttc ctcctaatta tttccctttt ttatattcct gcttattttg cagttgtaga
     8581 actagttgat tgatctgttt catttgtcga gctgtctgta tttgattcta atataggata
     8641 aactttttca ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag ctacatactc
     8701 atcatctttc agcaatgaaa catcttggtg cagctgatca acgtctgcgt tgacttcttt
     8761 catttcagta tttgcttcga ctctcaattc gttcaattga tgcatccttt gcatgtcatg
     8821 gaaaatctgc acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa ttgcagccag
     8881 tctgcgtctt cgaaaaatca gttgacgttg ttgttgctga aattcagcat actttttctt
     8941 tgcatattcc gtatctagcg cagcgatttt attgcttttt ttcgtcactt ggattcgctc
     9001 caatcttttt gattgtcttt ttcattatac caatatcgtg aaagcttgtc taatctattt
     9061 ctctagtgtt tctgtgcgat tttcagaaat aatttcgtac attttttgtg catcttcttt
     9121 tttggttgat tcatgtaatt ccgtaatttt cacttccaac gttttgttgc caaactggac
     9181 tttcaaaata tcgcctactt tgacatcact ggaagatttc gccaatttac cgttgatttg
     9241 aattcttcct ttatcagcta cttcttttgc tactgatcgt cttttgatga ttcgggaaat
     9301 ttttaaaaat ttatctaatc gcatatctta ttcctcactt tttaattttt ttatttaatc
     9361 gcaggatttt cttaccaaaa ggcaacatta gccattctcg tactgtaaac agacgaagac
     9421 gaatcactgc ataaatgaac acagaagccc cgatcccaac tcctatcacg ctgacaaaaa
     9481 aagcagtgga acgatgagtt acaggaccaa atactatatt caatacacca taatacagca
     9541 gcagtactac tgtcatacaa agaaggctga acatcaactt ccgtccaaaa tgccgttctt
     9601 tccaaaaagc atttagctct tttgtttcaa ttcgaacaaa ataccaaagc gtcacaccta
     9661 atcctaaaag ggtagacagg cttgcacctg ctgttcctaa taaaccggtc agaaaagaag
     9721 tagtcaatcc ttttaccaat agtccccatc ctgcgccttt taaagaaggg cgaaacgagt
     9781 tcttgctttg agcaatactt tgataagctt ggatgactgc agtgaaagca atggaaaaaa
     9841 caaataatac aagcgctgca tttcccgcat aatctttaaa caacgcataa ttgatataag
     9901 ggagcagcaa ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc aatctaagat
     9961 agattttggc tgtctgtaaa aactgacgat agatccggtt ggtcaaatga cgagtcaacg
    10021 caggcaggaa tgtcgcactt aatgcagtgg caatcactaa acctaattgt actaaaggct
    10081 gacctcgatc atagacccct tttgctatct tcgcaccgta ttctgacagt ccgtaaactt
    10141 ccaaagcatt ttttacaaag aaagagtcga tcagctggaa aaagataagc aatccactat
    10201 aaatcgaaac cagtccgcct tctaccagaa aacgccggat caagggcttt gacggctgtt
    10261 tgaacaatgg aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt tcatagtagt
    10321 gaaggatccc atatgcgcat aaaccgccaa aaacagctcc actcattgct gccgtccctg
    10381 tttggtacac cgtccatcca agctttctaa aagcaaacgc actacagaca atcacaaaaa
    10441 cacgaacgaa ctgctcaact acttgagaaa cagcagttgg ttccatcaag aatcttcctt
    10501 ggaaattccc tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag gaaactactt
    10561 ggatcagcgg tttcaattgt atgtctccca tcatccaagc aatccagctg ctaaatgcaa
    10621 agacaaacaa ccacaaaaag acacctgtcc aaaatactaa gggataaaga ttttgcaaag
    10681 cttgctttcg ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg aactgcggaa
    10741 gaccggataa agctaaagtc attgctatcc cataaattgg ataaacttgc tgataaacat
    10801 aaaaaccttc gtcgccgact aagttttgga ggggaattcg gtaaagggca cttaatactt
    10861 ttgctatgaa agaagcaatc gtcaatacaa atgctccttg catgacgcgc cgcatttctt
    10921 tatgatccaa atcattgcct acgctgacag atatttttgc tcacgcaacg ctttgacaaa
    10981 cttttggatt tcctgaagcc atactgcttc ttccatttgg ttggggatcg tcaagcgtat
    11041 caccatctgt tctttttcaa cgcctagact ggcttttagt tttgtcgctg tcagtgcttc
    11101 gaataactgt tccacactat agcgttttgt tccaacttta cttagagtaa agactatatg
    11161 cgcctcctgt ttccttatcg tttctaggag cgctcgatcg ccatccattt tgatctgccc
    11221 aattgtcaag agatgtgcta cttcttctgg atattctcca aaacggtcga gcagatcatc
    11281 ttctagttca tctagcattt cttgcgaatc aagttcacga attcgtttat agatctcgat
    11341 tttctgccgt tgatctgcca cgtatgtttc aggtagatac gcatcgacac cgaggtcgat
    11401 ctcaacagtg gtcttctcca cttgactatt ctttccttgt ttacgagaaa cagcttcgct
    11461 tagcatttga gaatacatat cgaaaccgac tgcatcaata aatccatgct gctgagcacc
    11521 taagagatta cctgcaccgc gaatggaaag atcccgcatc gcaatcttga atccagatcc
    11581 cagttctgta aaatctttga ttgcctgaag acgtttttca ctgacttcgt taaggatctt
    11641 ctgctgctca tacataaagt aagcatatgc gacacggctg cttcttccta cccgccctcg
    11701 caattggtat aacgtggata atcccatata atcagcgttc tcaacgaaca acgtatttgc
    11761 gttcgggata tctactcctg tttcaatgat ggttgtagtg acaagaacat catattgacc
    11821 ttcaataaat tcaaaaagcg tattttctaa ttgcgcctct gtcatttgcc catgagcata
    11881 accgatccgt gcttccggaa cgagttcttg gatttcttct actttttgtt cgatcgtttc
    11941 tacccgatta tataaataga atacttgtcc acctcgtccc atttctcgat ggattgcttc
    12001 gcgaatagcc ccaggatttt tttccataac atacgtttgg atcggataac gattcgcagg
    12061 cggtgtttcg attactgaaa gatcccgcac gcctaacatc gacatatgca aagttctcgg
    12121 aatcggtgtt gcagtcagag tcaaaacatc cacttgtgca cgtaactgtt tcaatcgttc
    12181 cttatgtttt acaccaaaac gttgttcttc gtcaatgatc agtagaccca aatcgctaaa
    12241 ttcaatatct tttgacaaaa ttcggtgggt tcctacaaca atatcaactt gacctgtacg
    12301 cagttgttcg atggtttctt tttgttgctt tcttgttctg aaacgactta atagcccgat
    12361 atttacgggg aaaccttcaa atctttccag catcgtttca tagtgctgct gtgccaagat
    12421 cgttgttgga actaaaaagg ctacttgttt gctttccttt atcgctttaa aggccgctcg
    12481 aagcgctact tctgttttcc cataaccaac atcacctacg agtagtcgat ccattggttt
    12541 ttctttttcc atatcccgtt tgatctccgc tgcacttctc aactggtcat cagtttcaga
    12601 gtaggggaag gcattttcga attctttttg gtaggcgtca tcaggtccaa aagcatatcc
    12661 tttttctgac tctcttgcgg catataattt gattagatcg tcagcgatat cctcgatttt
    12721 tgaagagact tttcgcttgg ttttcgtcca ttcacttccg cctagcttat tgatgcgagg
    12781 cgctttggct tcagaggcga catatttttg gatcagattc agttgagtaa caggaataaa
    12841 cagtttgtcg tcgttttgat acaaaatagt catatagtct tggtgaacgc catctacttc
    12901 tagcgtttcc atcccaatgt atttcccgat accatgattg gcatggacaa cgtaatctcc
    12961 tgcttttaac tcattatagc ttttcaatcg ctctgcgttt gttactgttt gacgtctcgt
    13021 ttgttttttc gtcgtttttt ggaaaatttc tttttctgtg atagctacta ttttttcttg
    13081 aggaagttca aaaccagcct gcaaagctcc ttcaactaat tgtacctgtc ctctgatcaa
    13141 tgcatttttt tctgtttcta ctactaatat gtcttcatct cgaagcattt gttctgcttt
    13201 gcgtattctg tcctttgtcg gaataaagat cacaacagtc tgtttttgct tgcgccaacg
    13261 gtccatttct gttttcagca atggcatttg tccgaagaac tgttgcatcg tccgatattg
    13321 aaaattatgg attgcttgga aacgaagatt tcccatccct ttttggaaga gtgagaaaaa
    13381 ggttgtggtg aacttttcct tctgcacgat tttttgtata tctgctgaaa atgtctgctc
    13441 agaaaacacc cgcaattcgc tgattttttg agtctgccat tctgccgctt ccctttcgat
    13501 ttcccgattt gtttccatga ttcgctgata atcatcaacg actagtaaac tatttgaagg
    13561 gaaataatcc aagatcgtca cagattcttg gtaaagaaga tctgagtaga attttgcttc
    13621 ttctgtcggg acacctgctt gccagcttga agtcagctga ccgaagtaat cttgcaaaaa
    13681 ctcgcggtct gcttcttctg tggtcgcagc aagccgtttt tctagcattt tttgcatttg
    13741 ctctattcca tgttctagat cctctggtcc gtagacttgt tcactcgttg gggaaaccca
    13801 gatttcttcc gttttttcga ttgagcgttg tgtttctgct tcaaaatacc gcatagaatc
    13861 tatctctaca tcaaatagtt ctacacgaac aggataggtt gtcgttaacg ggtaaatatc
    13921 gataatgctt cctcgaatac tgaattctcc cggttttgcg accatctctt ctcgtacata
    13981 gcccatcaaa gtcaattggc gaggaagatt ttccacgtct atctcatcac caatatgcca
    14041 attcaattgc gcctgtagcc aagttttctt attcggcagt cgtttgtgca aagatgcgac
    14101 tggcaacaaa tagatacctg cttttttttc ttgaagtgcg ttaagcgttg caactctctc
    14161 gcctcgcgct tctggagaag aaaaggccat ttcagcagaa agcacttcat ccactggaaa
    14221 taaatggatc tgatcttgcg gaatcacatg ttgcagatct tctgctaact gattcgcata
    14281 atacaaattt ggcatcagaa taatcatgtt tttatttgtt ttcttaaacg ctcgagcaaa
    14341 tagaagtgtt ttcgctgagc ccgctaatcc tgtgattagt tgtctaggca ttttttcttc
    14401 taaattgctg acccattctt ttacttgcgg atcttttccg actaattgaa taatatccat
    14461 tccttttcct ccttcctcaa ttgaatcgat tcatcgcatc cacgaacgaa tgcccttcta
    14521 tgagatattc gactgcttta acagattgac acatgctttc ttctatcaaa ggctgatttt
    14581 ctttgctgaa aggactaagc acatgctgga ctactgtttt tttcccttct ggcctgccga
    14641 ttcccacctt gattcgatca aagacattcg tattcaaatg ggaaataata cttttgatac
    14701 cgttatgccc gccggcactt cccttttgac ggagccggat ttttcccact gccagatcca
    14761 aatcatcgta gattacgact aattcctctg gataaatccc aaaataagtc attagtgggc
    14821 cgactgcacg accagattca ttcatgaatg tttgtggttt gactaaaagg atcttttctc
    14881 cattatgaaa aaattcggca acttctgctt cgaacggatt ttttttgaaa gtagcttgat
    14941 gctcgcgtgc aattttatcc acgaccataa agccgatatt gtgttttgta tattggtatt
    15001 tggtacctgg gtttcctaac ccgacgatca ttttcatttt tgttttccac ctaattctaa
    15061 aaagcagcac aaaaggctgg actgctaatt agcggtccag tccatcgagc ttttttcttt
    15121 tttatacttg tcaatgcata gtataacaca aaacaaggcg tttcggctaa aagagaatcg
    15181 ttaataaatg ataaagaaat taaaaatttc ttcaaagttt aatgaaataa ttgatatata
    15241 aaaatagtga aataacgcac atgtaagctt ttacatataa tacaaccttg ttatctgtta
    15301 tattttatag ccaaatagcg tgacatgggg cttgagaaat gttagcataa gcatgggaaa
    15361 aaacaaatca atggaaggat tgatactaaa tgactgcagc tgcagaaaaa aaagatcatc
    15421 aaaaagttat tcttgttgga gacggcgccg taggttctag ctatgccttt gctctagtta
    15481 ctcaaaatat cgctcaagaa gtaggtattg tggatatcaa tactgctaaa actgaaggag
    15541 acgcaattga cttgtctcac gctttggcat ttacttcacc taaaaaaatc tattctgcta
    15601 cttacgcaga cgcacatgat gctgacttag ttgttattac agctggcgca ccacaaaaac
    15661 caggtgaaac tcgtttagac ttagtaaaca aaaacctaaa aatcaaccgc gatgttgtca
    15721 cacaaatcgt tgaatcagga tttaacggta tctttttagt tgccgctaac ccagtagata
    15781 ttttgactta ctcaacttgg aaattctcag gtttccctaa agaacgcgta atcggttcag
    15841 gtacctctct tgactcagct cgtttccgtc aagcacttgc ggaattagtt gacgttgatg
    15901 cacgtaacgt ccatgcttac atcttaggtg aacacggaga ttcagaattc ccagtttggt
    15961 cacatgcaaa tgttgccggc ttgcaaatct atgaatgggt aaaaaacaac ccagaaatcg
    16021 atgaagaagc tatggttaac ctattctttg gcgtacgtga cgctgcttac acaattatcg
    16081 agaaaaaagg cgctactttc tatggtatcg cagtagcatt agcacgtatc actcgtgcca
    16141 tcttagatga tgaaaatgct gttcttcctt tatcagtatt catgaatggc gaatatggat
    16201 tgaacgacat ctacattggt gcacctgctg ttatcaaccg tcaaggtatc caaaaagtaa
    16261 tcgaaattcc tttaagtgac agcgagcaag accgtatggc tgcttctgct aagcaattaa
    16321 aagaaattct agatgaagct tttgctaaac tagacgctga ataatctttg aacatgacgg
    16381 aaaacatcta ttcggtgttt tccgtttttt tattttatca cccttatggt ttctaaattt
    16441 tttcataaag tagagataac cttcgtcttt tcctctaaat tatgctatca tgagaccgtt
    16501 ccaccctaaa cttttactta aggagttgtc gatatgacaa gatcttcgca tcgaaaaaaa
    16561 tcgaaacatg ttgctttgct ttcagttctt ggtgttctct tagtgctgat cagcttttat
    16621 acctatcgca gtacatacta cacaaagcat tttttgccga atacagaaat aaatggaata
    16681 aatgtcagca atctaacagt cgaaaaagca aatgaaaaat taaaagaagc ttattctgat
    16741 aaaaaactgg ctattaaaga agatggaaaa gtctggcagg aagtaccaaa atccgagctc
    16801 ggctacaaag atgatttcac ttcggagctt tctccgatcc taaatgaaca aaacggatgg
    16861 acttggggaa tgacttatgt ctcagcggca gaaaaacaag aaatagatcc tgtcagtcaa
    16921 aaccaccaaa aactagacac taccgttcag acattgacaa ctaagctgac tgatttgaac
    16981 aaagaccgga cacctactac tgatgcaacg atcgaaaaag caggtgactc tttcgttatc
    17041 aagcctgaag tcaatggaaa tacgatcgac gtcgatgctg cggtcaaaca attaaaaagt
    17101 gcggtaaact ctggtaaaga tacaattgaa ttgactgaat tcaaagaaaa accaaaagta
    17161 acatctgaag acagctcact gaaagaacaa ctcgcatcca tgaatgctat cgcaaatgta
    17221 aaagctactt acagtataaa cggtgagact ttccagatcc catcaagtga tattatgagt
    17281 tggctgacct acaatgatgg aaaagtcgat ttagatacag aacaagtacg tcaatatgta
    17341 acagatttag gcacaaagta taacacaagt acgaatgaca caaaatttaa aagtacaaaa
    17401 agaggagaag ttacggtccc agttggcact tacagttgga cgatccaaac agacagtgag
    17461 acagaagcat tgaaaaaagc gattctggca ggccaagatt ttactcgttc acccattgtg
    17521 caaggtggca caaccgctga tcatccattg atcgaagata cgtatatcga agttgatctg
    17581 gagaaccagc atatgtggta ttacaaagac ggaaaagtgg cccttgaaac agatatcgtt
    17641 tccggaaagc caactacacc tacacctgct ggagtcttct atgtttggaa taaagaagaa
    17701 gatgcaactt taaaaggaac aaatgacgat ggaacacctt acgagagtcc ggtaaactac
    17761 tggatgccga ttgactggac aggtgttggt atccacgatt cagactggca acctgaatac
    17821 ggtggagact tatggaaaac ccgcggttca cacggctgca tcaacacccc accaagtgta
    17881 atgaaggaat tattcgggat ggtcgaaaaa ggaacgcctg tattggtctt ctaacaaaaa
    17941 taatatataa aaagaaagca cccgtacgat tggtacaggt gctttctttt tattatctaa
    18001 caatgtgcta cttacatttc agcagcagta aagacttctg aaacatcatc atcgtcttct
    18061 agcttatcta ctagacgttc caattgctct ttttgttcct catttaaatc aatcgtcgtt
    18121 tgtggaatca tcgtcaattc tgcttgcgcc aaagtgaact ctttttccaa ctcatctcgt
    18181 acagcagcaa aatcttctgg tgcagtataa atctcgaata cttcaggaga agtttgcaga
    18241 tcttctgccc ccgcctctaa tacatcctca aacatcgtct cttcatcaac agtcaaccca
    18301 tcgcgttcaa tagcgatata tcctttgcgg tcaaacaagt aactgactga acccgtttct
    18361 cccagtgatc caccgtttcg agtaaaagct actcggacat ttgtcgctgt ccgattacgg
    18421 ttatctgtca atgcatggac taagattcct actccacccg gaccatatcc ttcatacgtg
    18481 atttcatcgt aattcgtttc atctacggca ctcgtagcct ttttaatcgc ccgttccaca
    18541 ttgtcgttag gcatatttgc agcttttgct ttgtccacaa caagccgcaa agctggattc
    18601 atagatggat caggaccgcc tgctttagct gccatataaa tttcacgaga aagtttctga
    18661 aagactttcc ctcgttttgc atcttgggca ttttttcttc cttggatatt atgccattta
    18721 ctatgtcctg acatgatttt tcctcccttg ttcttttgag aatacacttc tattctagta
    18781 ctatttcaca aaaaaacaaa gccacagcca atctataaac gcttcttgaa tgccgcaaaa
    18841 agcacacata atatcacagc agtcagagca ccgatcgaat ccaacatgac atcttggaac
    18901 ataggtgttc gtccaccggt catcatctga tggaattcgt ccaaacctgc gtaccctgtt
    18961 gctgaaagcc aagcaaggat cgctgtcagc caccaaattt ttagcttggg gaaaacacct
    19021 aaaaacaagc tgcctcccaa aataaaatag gtaccgaaat gtgcgccctt ccttaaaaag
    19081 aactctaaaa atttggcgta gcccagatgg tcgatactca ccatacttcc accataatta
    19141 aaacgtatac ccgctaatgt gtctttaaat ggctgattag gcagccagct ttctaatcgc
    19201 gaaacttgtg attgctgtcc gtaagtttga gaagaactgt aaaataagat tataacgact
    19261 atcagtgcga ttactaaata aatatttccg tttttcattt gtttttttga agaattcata
    19321 agtcacctct gtattttatc ttagactttt tttgaagaaa caacaatcat tttcatagag
    19381 acttcttcca taaaaaagtg ctatacttaa ttttgtatta caatatgaag ggagtttttt
    19441 catgagttat tcattaaatt ggaatctaga ttctattttt tccggaggca gtcattctga
    19501 tgctttgaac caacggatga agcaattgga agaccaaatg aacgagtatt atcaccgcgt
    19561 aacaaaatgg tcacctagtt ctgacaatgc agagcagtta aacgctattc ttcaattgca
    19621 agaaacgatt acgaatggtt ttacccaatg cagcagctat attactgcat tgctttctgc
    19681 caatgtcaat gattcagatg caaaggtctt atcaggcaag ctttacgcaa tgctgcctcg
    19741 tctgcaatca gccgaaactg tattgtcaaa gaaatttgca gaaatatctg acaatgattg
    19801 gaatcagatg ctttctcaat cttcgtttga aacaattgct tttcgtttaa acgagattcg
    19861 tcgtgatggc tcacaattat tatcagaagc tgaagaaaac attataaata cgctttcttt
    19921 agatggtcta aatgcttgga gcagtcatta tgatacgatt gtagccagca tttcgattcc
    19981 cttcgaacaa gatggacaag tagtcgaatt atctgctgga caagccttca ataagatgat
    20041 gggcgatcca gatccaaaag tcagagaaac acttttcgca gcatgggaag aagcctggaa
    20101 agaaaaggca cccctcttca cggatacttt gaatcattta gatggtttta gactttctga
    20161 ctatgaactt catggtgtca cagattttct acaaaaacca ttagagtaca atcggttgaa
    20221 gaaagaaaca ttgtctgtca tgtgggatac aatccagaaa aacaaacaac cccttgttaa
    20281 ttacttgact agaaaagcta atcttttcgg taaagaaaaa atggaatggc aagatcaaga
    20341 tgcacctatt attttaggcg atttgaaaga aaaaaccttt agttttgacg aagcagcagc
    20401 atttattatc gaaaacttta acaaattcag tccaaaaatg gctaaatttg cccaatctgc
    20461 ttttgaaaag agctggatcg aagcagaaga tcgtcctggg aaacgtcctg gtggttattg
    20521 tactgagtta ccagaaacaa aagaatcaag gatctttatg acctacagta attctgttaa
    20581 tgaggttgcg acacttgctc atgaactagg acatgctttc catagcagca cgatgtggga
    20641 tttaccttct ttaaatcgcg aatatgccat gaatgtcgca gaaacagcaa gtacattcgc
    20701 agaactgatc gtagccgatg ctacattaaa agtcgcaaaa acaaaagaag aaaaaatcaa
    20761 tttgctagat acaaaactgc aaaatgcttt agctatgttc atgaatatcc attcccgatt
    20821 catttttgaa aatcgattct atgaagcacg gcaagaagga ttagtctcag aagataagat
    20881 cacagagatg atggttgagg cgcaaaaaga aggctatcaa ggagctttag ctacttacca
    20941 tccatatttc tgggctgcaa aattgcattt ctttattgat gacgtaccgt tttataactt
    21001 cccatacaca tttggctacc tctttagttt gggcatctat gcttacgcca accaaaaggg
    21061 agcaaatttt gaagaagaat atatccattt actacgcgat actgcctcaa tgacgacgga
    21121 agatcttgcg aagaaacatt tgaatgtcga tcttacccaa ccagattttt ggcaagcagg
    21181 tatcgacatg gtgactgaag ata
//
LOCUS       DACEKH010000044        21005 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEKH010000044 DACEKH010000000
VERSION     DACEKH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.045"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            201..3479
                     /locus_tag="IW662_001515"
     CDS             201..3479
                     /locus_tag="IW662_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ0866346.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(4020..5927)
                     /locus_tag="IW662_001516"
     CDS             complement(4020..5927)
                     /locus_tag="IW662_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ0866347.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6057..6932
                     /locus_tag="IW662_001517"
     CDS             6057..6932
                     /locus_tag="IW662_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ0866348.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSEKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            7012..7986
                     /locus_tag="IW662_001518"
     CDS             7012..7986
                     /locus_tag="IW662_001518"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0866349.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8175..9311
                     /locus_tag="IW662_001519"
     CDS             8175..9311
                     /locus_tag="IW662_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0866350.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9448..9792
                     /locus_tag="IW662_001520"
     CDS             9448..9792
                     /locus_tag="IW662_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ0866351.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10063..11349
                     /gene="efmA"
                     /locus_tag="IW662_001521"
     CDS             10063..11349
                     /gene="efmA"
                     /locus_tag="IW662_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ0866352.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11487..12086)
                     /locus_tag="IW662_001522"
     CDS             complement(11487..12086)
                     /locus_tag="IW662_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ0866353.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12490..13611
                     /gene="mnmA"
                     /locus_tag="IW662_001523"
     CDS             12490..13611
                     /gene="mnmA"
                     /locus_tag="IW662_001523"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ0866354.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13857..15278
                     /gene="arcD"
                     /locus_tag="IW662_001524"
     CDS             13857..15278
                     /gene="arcD"
                     /locus_tag="IW662_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ0866355.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NATTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15292..16410
                     /locus_tag="IW662_001525"
     CDS             15292..16410
                     /locus_tag="IW662_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ0866356.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16470..17762)
                     /locus_tag="IW662_001526"
     CDS             complement(16470..17762)
                     /locus_tag="IW662_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0866357.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17912..18472
                     /locus_tag="IW662_001527"
     CDS             17912..18472
                     /locus_tag="IW662_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0866358.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18601..18978
                     /locus_tag="IW662_001528"
     CDS             18601..18978
                     /locus_tag="IW662_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ0866359.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18911..20983
                     /locus_tag="IW662_001529"
     CDS             18911..20983
                     /locus_tag="IW662_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0866360.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         6806 a         3803 c         4468 g         5928 t
ORIGIN      
        1 ttttatgctc ttttttatac gttattttaa aaaaacttat tctttttacg ttaataaatc
       61 tctttattat caacaactta agttatattt tttcttttta taaaatacac ttattataat
      121 tttgtttttt attgacagta aaaataaatt aggtaatact ttaagtaaac gctttctaat
      181 ttgttacggg aggaatacac ttgttaaaaa aacgtataaa atacagtgcg ctaatgatga
      241 atctccttat gctcagtacc ccggttttaa tttctgtcgt tgaagttgct gctaacgaac
      301 aacagtctgt gaacgaagaa aatgcacacg tgaacattga agggatgtca acaatggagg
      361 aaataccaag aactagtgaa gatatggata cggaagaagg aaatgatcct atgatgaaag
      421 aatcagaaaa tgaaaagaca aaaaagcaat ctgaagaagc aaatgaagag tggaaagggg
      481 ttgttttcgg agaaagcaca agtgctagta ggtatgccat tgaaccatta gaagacggcg
      541 tgcggctctc ttccacgaat aacggcggaa aattccagtc aagcggatca gatggaatga
      601 cctattatta tcaagcaatc cccaaagaca tcaactttag gatgcgggcg acgatcgaaa
      661 tagaatcttg gacctatacg aatgcccaag aaggatttgc tttaatggta agagacgcag
      721 taccaaaaaa tcatttgttt gggcaggcat tttattcaaa ttcatttgcc atactaggca
      781 gtcggatcga atatgtctgg gactcggata gacaagaagt ggtcaacacg agcggcagca
      841 aatatacgat gcgacttgga ttggggacac gggcaatcac aggaatatca tctgaagatc
      901 cgcaagctgc gccagctcct gggagtgtct cagtggaaac aacaccttta gagacttcag
      961 cagggttctt ggaaaaagaa acaggttctt ataatatatt cggaaacggt catgggaatc
     1021 tgtttccggc cacgaactcg attactaaac tggacgtaga gatgaaaaaa acgaatactg
     1081 gtttagaggc atattattac gatcctgaaa caggagaaga aatggcttct gacatcatgt
     1141 atgactggga aaaattatac gcttcagatg aatcggttat ttatgcagga tttgctgcag
     1201 caaggaatat gacgatcaag gtagaaggaa tcgaagttgc ttactcagat cctgcgacag
     1261 atcctcctgg gcaagaaaga cctactcgct tgattgatcc gatttacact gtgacctcta
     1321 gtaatcattc aggaaaccaa gatcatacta tgtcctttag agcaaatgct gacggattat
     1381 tgacaattaa aaataaagca acaggagagg tgcttaagaa cgcaaaaaac ttggctatca
     1441 ctgcgaatcg ggaatttgat tatcagacaa aactgatgga aggaacgaac gaatttactt
     1501 tcagttttac gccagataaa aactatccgc cagaagaata cgtagagatg actagttatg
     1561 aaacaaaaga gattcttcat acagtggact accgtaaacc taaatattcg acagtttatg
     1621 taacaccaga agggtcagat gcaggaaacg gaagcagaca aaatccatta tctctgacac
     1681 aagcattgag atacgcatat gctgggcaga caatcgtcat ggcgcctgga acttattcct
     1741 tcgaaagagg attgacgatt ccaaaaggcg ttaacgggac aagagaaaac ccgattcagt
     1801 tgattgcaga gaaaaatcca gaaaacaagc gtcctgtact tgattttaaa ggaactggaa
     1861 atgggatgac actgttcagt cactactggg gtttacgagg gtttgacatt acaaatagtg
     1921 cagctatgca aaaaggatta cagatttctg gaaatcataa tttgatcgaa gagattcatg
     1981 cttatcggaa tggaaataca ggaatccaaa tcagcggtag ctcaaatgat cctttcgaag
     2041 catggcccgc gcataactta gtcaagaact gtacatcttt tgaaaatgcc gatccaggat
     2101 ttgaagatgc cgatggattt gcggccaaac tgacaatagg agaaggaaat gtatttgacg
     2161 gttgtatcgc gtatcataat gcagatgacg gttgggacct ttttgctaaa aatgaaacag
     2221 gttcgatcgg aaaagtaatc atcaaaaact cagttgctta tcgaaacggc tgggtaccag
     2281 gaattgaggg agaaggtaac ggtaatggat tcaaaatggg cggttcttct ttaacgggcc
     2341 ctcatgaatt gatcaatagt ttgtctttcg aaaatcttgc gaaaggaatc gattccaata
     2401 gcggaacaga tattatcgtc aatagcagta caagttttaa taacggttca tataatgtgg
     2461 ctctttatac ctcttcagca ggaaacacag actaccatgc ttctggtatc ttatctttcc
     2521 gaacagagaa tcttgagatg agagagcaga tccgcccatt gaatcaaaac gaagatcaaa
     2581 tctaccactc gttgaactac tattggaatg aacgagagaa acaatcagaa aatactcttg
     2641 gacataccat atcaaaagat tggatcgagt ctctaaatac atgttcgaaa gaaggtcagc
     2701 agcctgttac gcgctatgaa aatggaagta tccatgttca tggactgatg cagattaaac
     2761 cggggaatcg tcaaacagaa aaggaaagag tggatggtct tccattatct gtaggtgcta
     2821 tttttgacgg agaaacctca ccgtcttcgg aatatccaga gtatgcaatc gtacaagaac
     2881 caaatcagac aaaaccaaat gaacaaaata atccgattat tatacaagaa gcaaatggtg
     2941 tggcggagaa tgcagaagga aatatcattg aactgaagcc ttttacagtt tttgtgtcaa
     3001 ctttcacacc aaatcaagga acgcctgtct ttgaagatat gcgttatttc ccagaaaaga
     3061 tccaaatcgt tgaaacacag tcaagtgctc aattttctaa tcataccgct attttttcag
     3121 caccttcaaa aggagaatat acgctagaag tcacttatcg ttcggaaata tttgatgggg
     3181 aaaaatggtc tgctcataca gaatctgtta cccagcaaaa agcgatcact gtgcgagaaa
     3241 ctgccgatgt gcccccggga aatgacaaac cagaaagaga aaatccagga aatgaatcac
     3301 caaatggaaa accaaatgga aaaccttctc atccggatag gacagcaaaa gaaagtgaaa
     3361 aaaatggcac tcaaggactc ccaaaaacgg gagaatttaa taacccgttg ctagcattag
     3421 ctggaggaat tttgttaatc ggtgtagttg tttatgtcat gaaacagcga aaaaaataag
     3481 tggaatttct ccttttatga tgtaaaacaa ttgactgagg aaagtaaaaa tagaaggaaa
     3541 tgagaccaat agatgtctca cttccttcta tctttttata gatgaggttc tccttgctgc
     3601 ttttgattgg ctgttcttat tacttgatgc aggacagctt tttcacaatc tctccactaa
     3661 acggtgttca atcagctgac cctcggtatt agctcaacaa acggcaaaat atgaggaact
     3721 atttttccgt ttttttctct aatactcagg tcaggacgct gatttcacaa cctctcgata
     3781 aacggtgttc aaaagccagt ttctcggtag taagtcaaat ttatgcaaaa atgtggaaag
     3841 ccattttcac aaaatttttc ttactacttg aaactaagca gctttttcac aacctctaat
     3901 acatggtatt ccagaagtaa cttcttcgaa aaataagccg aaatgtcaca aaaatttgag
     3961 aagcgatttt cccaaattcc ttcttatttc ttgaagctga ccacttctgg cacaacctct
     4021 taccagattc gcactctttt ttctggcgct agatacatag cgtcttgttc cgtgatacca
     4081 aatgtttcat agaattcgtc aaggtttttt ggttgcacgt ttgctcggag tttagcaggg
     4141 gcatggacat cgatttgcag taaaagttgt tggtattctt tttttgcttt tgtacgccaa
     4201 atcgttgccc aattgacaaa gaattcctcg atagaaagat ctgattccgt ttttgctgct
     4261 tccaatgcac agcttagacc gcctgcatca gcaatatttt ccgaaacggt taattttccg
     4321 tttacttttc cgtcagcaaa aggcagacca tcgaattctt cgatcattgc ttgtgctttg
     4381 ttttgaaatt gtgtaagatc ttcttctgtc caccagttgt tcaagttgcc atattcgtca
     4441 aataaagcgc cgttattatc aaatgcatgg gaaatctcat gagcgataac agctccgatt
     4501 ccaccataat tagcagagct tgattgttct agactgtaaa atggtgcttg aaggatcgct
     4561 gccggaaaga cgatcacatt ccgaaatgga tgataataag cattgaccgt gtcagcactc
     4621 atttcccatt ctgaacgatc cactggtttg ttccatttgc tgaaacgatc ttttaatgtg
     4681 agtttgctga aaacaagagt atttgaaagc aaagtaccac cctgatctgc aggtactgtt
     4741 ttgaactggt cgaataatgc tgggatctta tcggggtagc ctacttggat acctaagtta
     4801 tttaatttta tgacggcttt ttcttttgtt gcatccccaa gccagtcatt ttcttctaga
     4861 cgttttttat aaacggcgat catttttttc accatttgtt ctacgtcttt tttagcagct
     4921 tcgccaaaat atttttttcc gtagtaatcg ccaactactt gatcgaattg tccagaggca
     4981 aggtaatacg ctgctttttt ctgaggcatt gcttcgtctg ttccagacaa tacgcgagaa
     5041 tagattccgc tgacttggcg gaattcttct gatagataac cactaagaga acgtactgtt
     5101 ttaatgatca tccagctttt gaataacgga aaatgagctt ctgtcaggat gtcattcaaa
     5161 tgatcgaagt acactggatc agttacgatc actttatctg gagttgtccc aatcaaaccc
     5221 gtgacaagtt ttttcaagtc cagtttatct gtgtatttaa taaatgtatc aaaagattgc
     5281 ggattataca ttttgctgta atctgcgttt tcttctgcac tttttacatg aggagcaatc
     5341 aatttatcaa attgcactgc ttgttccgtg attgcttctg cctgactttt ttctttgccg
     5401 gcaagctcta ataactgaac agccatatca aagaaaacgc ctattagttg tgcaccgttg
     5461 ggatggtctg atgcataata cgtcttatct ggaaggaata aagaaggcgg ataagcaaag
     5521 aaggcgttcg tttgcgcatt cttcatatct gcatctacat ccagattaaa tggcaatggt
     5581 aatgtatcca acagccattc tggaaattgc ttattcaatt cgtcgaaaga ctgcaatgat
     5641 tcaacttttt cgattatagg ttgaagcggt tgagcatgca actcatttct tcggtcataa
     5701 tcgtttgcca gttgataata tgcaaggaaa tgtttcattt tttctgaaga cactttttct
     5761 ggatgggcaa gcagatcgtc tgtttctccc atcaaaagtt ggtcgatccc gtcaactaaa
     5821 tcttggaaac cgcctgtagc tggtttatct gcagggattt ttgctgtttt ttgccattct
     5881 tcattgactg cttcatagaa atcttgtttc aatcgttctt tgttcattca tcgatcgctc
     5941 cttttttgtt tcgtgtgccc attatcttac aaaatgaagg aattaacaaa tagaacataa
     6001 aaaattgaaa attcgaacaa tcaaactgat ttgttttttt caatccttca cttataatga
     6061 aaaaaaggag gcgatcccaa ctgaatcaag tagtggataa acctttatat ttcaaagtag
     6121 ataaaaaaat taaaaaatta gccagcactc agcagctgca gtcaagaaaa agtaaattgc
     6181 tttttttagc tctggttttt gaagatcaaa gttatgtgat tatcgatcaa tcaggacatc
     6241 cggtagaata ttctccagca aaatatactt atcaagaggg tctttcttgt aaaaaatgga
     6301 agctgatcaa tgaagagcca atcgagttat ctagatggat caacagaaaa gaagatattc
     6361 ccgttttgat cgaagaaaaa aggtcagaaa aggaactcgc aaattgctgg gtgggattgc
     6421 cagaagaacg ttttttgagg tataagaaat gggctacccc ctcaggctat ctttgtggta
     6481 cttacgcggc agcagttctc cttgcctatt atcaagacta tcgtaagggg tggatgcttc
     6541 caaatgagat tcgcaagaaa aatacggctg attcgttggt tttgacgaag gcattgcgta
     6601 gccagatcca accactagga cttcctacga ttccttttca ggttagtaca gggatttcta
     6661 gttttttaaa gaagaatggc aatcatgagc gtgcacgagc aactcttcta ggttcatggc
     6721 agcgagctac aaaacgtata cgtgaaggca aacctgtgat gattggtatt ttgaaagtac
     6781 ttggcagtac atatggcaat cattgggtga cagcgtacgc ttattttgaa actgaaacag
     6841 gagaacgtta ttacaaagta cacgacaact ggggcgatta tcataaagtc attcctgcca
     6901 gttggagtaa cggaacagtt tcacttcctt aagaatttgc tgaaattaac agaaaatctt
     6961 ggaaaaatat gttaaactat cgaagaatag agaagttttg gggaggacta catgagtaat
     7021 aagtatcagg atgacacgtt gaagattttt agtttgaatg gcaaccgtcc attagctgaa
     7081 aaaatcgcga aagtgttcgg aacagaatta ggtaaaagcg tcgtgaagca atttagtgat
     7141 ggcgagattt ccatcaatat cgaagaaagt attcgagggg atcatgtgta tatcgttcaa
     7201 tcaacgaatg cacctgtcaa tgattactat atggaactac tgatcatgat cgatgcaatg
     7261 aagcgagcaa gtgcgaaaac gatcaatgtc gtattgcctt actatgggta tgctaggcaa
     7321 gaccgcacgg cgaaacctca tgaaccgatc actgcgaaat tgatcgctaa cttgatcgaa
     7381 gaagcagggg caactcgagt actgacttta gatctgcaca cggttcaagt acaaggattc
     7441 tttgatattc ctgtagataa tttatttacg atgccgttgt ttgctcatta ttaccgtcaa
     7501 ttaggtctga cgggagatga catcgtagtt gtttcgccaa aaaacagcgg tgttcagcgt
     7561 gcacgtagtc tttcagaata tttgaacagc acattagcca tcgtagatca tgcagatgat
     7621 gcttcaactg agggtggtta tgtgatcggt gatgttcaag gaaagacatg tatcatggta
     7681 gatgatatct tgaacacagg agcaacgctt gcacgagcag caaatgtatt aaaagaaaac
     7741 ggcgcaaaag aagtatatgc ctgtgcgtca catggattat tgtccgatca tgcgaaaagt
     7801 attttagaca atgcaccaat caaagatatt tgtatcactg attctgtttt cacagaagaa
     7861 aatcgtcatc cagaaaactt aacaatcgtt acatgttcag aattaatggg tgaagcggtc
     7921 aaacgcatcc atgaaaatac accgatgagt ccgttattcc gtctggaaga aaaagaattc
     7981 gaataaacaa aaaaaagagg gaaagaagtt gtgacagaag tgagcagctt caaggagctg
     8041 cgacaagact tttgtcacag ctcctttttt ctatgcccat gaaacgtcct ctagacaaaa
     8101 atgagctctt ctttggtttg ttcgctattt tatgctagaa tgtgcctagc aataatttgg
     8161 aagggatgaa tgaagtgaaa actatttatc tggatcatgc ggcaactacc cccatgcatc
     8221 cacaagtaat tgaagcgatg acggcaatca tgcaagacac gtttggaaat ccttcaagca
     8281 tacatggatt tggtcgccac gcgcatgaaa aattggaaac agcaagacaa atcattgcag
     8341 acagtctgca agcaaaacct cacgagatca tctttaacag tgggggaaca gaaggggata
     8401 atacagctat cttagaaaca gcgttctcgc gtcaaaaaga aggcagacat ctaatcacta
     8461 cagctatcga acatcctgct gttcttaata ccatgaaata tttagagaca aaggggtttg
     8521 acgttacata tctgcctgta gatgaaaaag ggaatctgtc gatcgaagag gtcaaaaaag
     8581 cgttacgaga agatacgatc ttggtctctg tgatgtacgg gaacaatgaa atcggaaatt
     8641 tgatgccaat caaagaaatc ggtgaacttt tagcggatca tccagcttat ttccatacag
     8701 atgcggtaca agcgtacgga aaccaaacga tccatccaga agaattagga atcgatttat
     8761 taagtatctc tgctcataaa atcaatgggc caaaaggcgt cggtttctta tataaacgag
     8821 acggagtaaa tatccctgca ttgcttttag gcggggagca ggaagaaaag cggcgtgcag
     8881 gtacagaaaa tttagcaggg atctggggta tggcaaaagc agttgaactg ctgtcacctg
     8941 aagaacgtga agcacgtaac aacaaatata aaggattcca gcaattgatt cttgatacat
     9001 tagaagcaaa cgaaatccct tatcaagtaa atggtgattt aacgaataaa ctccctcatg
     9061 tgttgaatct tcgcttaatt ggcgaagcaa atgatttgct actgatgaag cttgatctta
     9121 atggggtcgc tatttctact ggttcagctt gtacagcagg aaatatcgag ccttcacacg
     9181 tattagaagc aatgtatgga gaagatacac cgatactaaa agaatccatc cgtatcagct
     9241 ttggtttggg aaataccgaa gacgacatcc agtatttcct tgatcaatta gttcgtgctg
     9301 tcaaaaaata actagtgaag tcattcttat atgaacagat gtctgagaga acgccttcat
     9361 tttttaggga acttaactga aaaacctagt aaaaatcagg ataaactttt ataataaaga
     9421 gaaactaatt ttatcgaggt gaaaatcatg gcatttgaaa caacagcaac ggtactgggc
     9481 gcaccaatta gctatcgttt gcatcctgat gcaaaaagat atacattgcg cgataatgga
     9541 tttacagaaa caaacggagg caattatcaa ttgatccgac cattagatgc cacgccgcaa
     9601 agcaaagaag gattcaaatt aaaaatcact gtatcaaaag atattcaaac attgaaaatg
     9661 tcgatcacta cagcaaatgg attgaaagca gtcaatattt tcaaagatcc aaaacagaaa
     9721 atgagccaag ataaatttta ttttctgatg gatgggatga ttagccgtgg gttattcgaa
     9781 aaagtcgaat gatctgtgga tataaaaaga caactagttt tgaaacggcc tattgttaga
     9841 tgactaactt tgtaaggacg acgcaaatta gttgtctttt ttatttttaa atttaacaca
     9901 tgttgttatt tttaatattt tgctgattta ttgtttctaa gtaaagaaat ttaaattgaa
     9961 acgattgtta tttagggaaa aaggtatttt ttgaaaaaag aatcccgtat aatgattgtt
    10021 gtgcaaacgc tatacaaaat gaaaaagaaa agagggatgt ttatggaaaa tgaacagtcg
    10081 gtcgttttaa cgaattggaa gagaaattat ttgttctttt tatcgggaca gtttttgtca
    10141 ggaatcacta gtatggtcgt ccaatatgcg atcatctggt atttgacaag agaaaccggt
    10201 tcggcgacaa ttttgagttt tgctacttta ctaggaatga ttcccatggt tttactaagc
    10261 ccatttgtag gaccgctagt ggatcgctgg gataaaaaag ctcttttgat tgtcacggat
    10321 attattgtag cgattttcgc gcttatttta gcagtagtcg gaacgatttc agaatccttc
    10381 ccgatttggc tagtttttgt gtctttgttt atgcgttcgg tcgcacaaac attccaaatg
    10441 ccaacgatcc agtcgattat gccgacaatc gttccatctt ctcacataac aaggacgaat
    10501 ggacagttag gaatggtcca atcagcgaat ttcatcattg cgccagctct aggtgcggct
    10561 ttgttttcag tcgttcctgt caattacctt attttattag atgtattggg ggcagttttt
    10621 ggtgtcggct tattgatttt tgtgaagatc ccaaaagttt ctcctgaaat attggaagtt
    10681 ccgcttacta tttttaaaga tgcaaaattt gggctgcagc aattgatgga taataaaggg
    10741 ttatggtaca taacgatcaa tggtgcattt gtgatgctgt tgttcatgcc agcaatcagt
    10801 ctgtatccat tgatgacgct agattacttt ggtggttccg ttggacaggc gggagcagtg
    10861 gaagtagtct acgcagtcgg aatgctctta ggcggtgcac tgatcagttt tataggaaca
    10921 tggaaagacc gaatgaaacc tattattata gcctacatca ttatgggcct gacgatcggt
    10981 gcaagcggat tggttccaaa cgatagtcaa gggttcttgt actttcttat cttgaatgca
    11041 ggagcaggtt gtgcaacgcc atatttcaat acactgctga tggcaatgat ccagcagagt
    11101 tatgaatcga atgttttagg tcgtgtctta gggaacttca actctttgat gaacctggca
    11161 ggtccaattg gattattatt tgcaggacca ttagctgatc gtttaggggt agaaaaaatg
    11221 ttcttgtttt ccggaatcgg gattttatta tgcggaatcg ttttattttt aacgtcagct
    11281 gcccgaaatt atgataaaga attacagaaa aaactggtaa aagaacacca tgagcaaaaa
    11341 gacgaatagt atggaatgga gttgtgacag aagtcttgtt acaactccat ttctgatcct
    11401 aatgttcctg tggcaataag cctactcaaa tgaaaaatat gagtaattat ctttatttgt
    11461 ttaatacttg atatagggca gttttttcac gacctatcgt acttcctctt tgcttcatag
    11521 atttctttga tttgttgctc agataacgga gtgccaaatg gtagtttttc ctttaagagt
    11581 tctctttccg gtgcgtctac gcctacattg acatcgttct cgataggtac gctgtaatga
    11641 tggatatgtc catgaagatt gcggatagtg tcgttaggcc cgagcaacat cggataatga
    11701 gtcaagtaat attgttgatg gtcgaattta acgatggcac ctacatcata aaaataaaat
    11761 ttgggacttc cgtcagtaag cctaggatca tttttttcta aataacggaa aaaagcgcga
    11821 ctgtcatggt tccctttgat caagtggatt gttccttgca gttgagtgac cagttccaaa
    11881 atttctgcat ttgctttttc atattgcgga tgcatagcaa ggtcgcctag atgataaaca
    11941 tggtcggttt cttttattac tgcattccag ctagtgatca actgttggtt catttcatcc
    12001 acatttgcaa agagccgcgg agcaaagtcg ttttttccta ataagcgttc atggaaaaag
    12061 tgcatatcac tgataaagta attcatgtat cttttgcctc ccttttttct tagagtaaac
    12121 aaaatcactt atctgagcaa ctcataacat ctaaaaagaa aaggttgtaa atgagacgtg
    12181 cagctttaag cgataagaag caattttgaa aaatagtttt tcacaattta taaaattatt
    12241 ccttattgcc gaaaggctgg ccctttttat gagtatgcca attttttata tcgaatgctc
    12301 aaaagatgag tgattttttg gttgactatc atgtttgtgt actgaatagg tccagtagaa
    12361 cagtattttt acgacttgat aattaaggga tgaaattttc agaaatatga cgtataatgt
    12421 aaaatactga aaagttgcga ccttcaactc gtagatgttt cagaatctaa ttgaaatgat
    12481 ggtgatatca tgacagacaa cagcaaaaca cgcgttgtcg tagggatgag cggcggtgtc
    12541 gactcatctg taacagcact tttgttaaag gaacaaggat acgacgtgat cggaatattc
    12601 atgaaaaatt gggatgacac ggacgaaaac ggtgtatgca cggccacgga agattataag
    12661 gatgtagcga aagtagctgc tcaaatcggg attccttatt actctgtgaa ttttgaaaaa
    12721 gaatattggg atcgtgtatt tgaatatttc ctagccgaat accgcgcagg acgtacacct
    12781 aacccagacg tgatgtgcaa caaagaaatc aaattcaaag cattcttgga ttatgcaatg
    12841 gatcttggcg ccgattatgt agctactgga cattatgcgc aagtcactcg cgatgaaaac
    12901 ggcgtggtcc acatgcttcg tggagtagac aatgataaag atcaaactta cttccttagc
    12961 caattatcac aagaacagct ttcaaaaacg atgttccctc taggcggcat ggaaaaatca
    13021 gaagtacggg caattgctga acgcgcaggc cttgcgactg ctaagaaaaa agattcaacg
    13081 ggaatctgct ttattggtga aaagaacttc aaacaatttt tgagcaacta tctgccagca
    13141 aaaaaaggaa atatggtcac tcttgatggt gaagtcaaag gacaacatgc tggcttgatg
    13201 tattatacga ttggtcaacg tcaaggacta gggatcggtg gcggaggaga ttcgcaagaa
    13261 ccctggtttg ttgtaggaaa agatctagcg acaaacacat tatatgtggg gcaagggttc
    13321 catcatcctg cattgtatgc cacaagcttg gatgcaagtg aaatccactt tacgacgaat
    13381 gaaccaatgc caaaagaatt caagtgtaca gcgaaattcc gttatcgtca gcaagacgtt
    13441 cctgtgacag ttcgcttatt ggatgataat cgtgcagaag tcgtattcga cgaacctgta
    13501 cgtgcaatca ctcctggaca agcagttgta ttttatgatg gaatggaatg tctgggtggt
    13561 ggtttgatcg accacgcata tcaagaaaca aaagttttac aatacgttta agcgatatta
    13621 aatgaatcaa aacagctatg gctaacaaaa gaaacacctt ttcttttgga tagcatagct
    13681 gtttttttgc aataaaatta atttttattc attttatact tgaaatactt gtgtaaaaag
    13741 ttcataatat ccaataatat gaataaagtt gctttttgtt aagcaagagc agttgcaaaa
    13801 tagaagggaa tcttttgcaa gtgatttgtt caattttata agtgagggtg atggaaatgg
    13861 aaaagaaatc gacgggtatt agcaaatggg gactggttgc tctggttgtc agttcgtcaa
    13921 tcggaagcgg tgtgtttggg atcacaagtg atctggctag ttctgcagct ccagggccag
    13981 cgatcctttc atggataatc gttgggattg gtattttagc tttggtatta tcgctgaatc
    14041 atttaggaga aaagcgtcca gatctagatg gtggtatttt cggttacgcg gaagcttcat
    14101 ttggtaaatt aggtggcttc atcagtggtt ggggttactg gttgtctgcg tggctgggaa
    14161 atgttgcttt tgcaacgatg ctgatgagcg ccatgggaga atttttcccg atattcaaag
    14221 gtggacaaaa tgttccttct attttatttg caagtatttt tatttggggt ctgactttat
    14281 tagtaaacaa tggagttgaa ggagcgagcg tcatcaatac aatcgtgacg atctgtaaat
    14341 tagtgccatt attcctattt ttaatatttg tatttatcgc atttaaagtg aatatattca
    14401 ctgctgattt ttggggtaac gtatctgata atctagtgaa tggaacaggg cagcaaggaa
    14461 cgatctggtt gcagattaaa ggttgtttga tggttatgat gtgggtcttt gtagggatcg
    14521 aaggcgcaag tgttttagcc aaccgcgcga agaaacgttc agaagcccag caagcatcga
    14581 tcattggtct gctttgttta ttgtttatct atatccttgc ttcgatcttg ccatacggcg
    14641 tcttatctca agaagagtta gcaactattt cacaacctgc tatggcaaat attttaaaag
    14701 gaatcgtggg aaattgggga gcagcgctga tcaatatcgg cttgattatt tcgatcattg
    14761 gttcttggtt gtcttggacg atgctcccag cagaaacaac gatgttgatg gctcgagacg
    14821 gtctgttgcc gaaaggttgg ggaaaaacga ataaaaaaaa tgccacaact tattcgttag
    14881 ttgttactgc aatcttgact aatctattct tattgacttt cttagttact gattatgcct
    14941 atcaatttgc ctattcttta tgtacagcgg caattttgat ttgttatcta cttgtcggga
    15001 tctatcaagt ccagttttct ttaaaagaag gaatcaaaaa acaagtagtg atcggtggga
    15061 ttgctgtagt ttttgaattg atgggcatca ttttagcagg attttcttat gtccttttat
    15121 gtagtattgc ctatcttcct ggtttttgct tctactggat cgcttgtcgt gaaaccaaac
    15181 atcaaattac gggaaaagaa aaagtaatga ttgggctgat ttgtgtggcc gcactgcttt
    15241 caatcgggtt gcttgctgta ggctggatca agatataagg gggaaagaaa catgaaaatc
    15301 gcaaatttcg gtgtagaaga atggctaaat aaatgggaaa agaaagcggt atatgatatt
    15361 gcacaaagct cgatcgaagc attaacatta gacgaactcg ttggcttaga tgggactagc
    15421 gtaagtaaat ttttcgaaaa acgcaaaaac gaaccattgg actatggctg gatcgaagga
    15481 tcagatgttt tcaaacaaga agtcgcttct ctttatcaaa ccgtcgatcc ggaaaacatc
    15541 ttacaaacga atggagcgac tggtgctaat cttttagctc tatacgcgtt agtggaagca
    15601 ggagaccatg ttgtttcgat gcttcctact tatcagcaat tatatgacat ccccaaatct
    15661 ttaggtgccg ctgttgactt tgttcatttg aaggaagaag aagattggca gtttgattta
    15721 gaacagctag aagccttagt caaacctgaa acaaaaatca tctgtttgaa tagtgcaaac
    15781 aatcctacag gcacattgct agatcggaaa actttagaaa aaatcgcaga aattgctcgt
    15841 acagtagatg catatgtatt gatagatgaa gtatatgcgc ctttaacgga taaaggtgaa
    15901 ttcttgtcga tcgtagatgt ctatgaaaaa ggtatagcaa ccaattccct atccaaaacc
    15961 tactccattc caggtattcg tattggctgg acagctactg gacctgagtt ggcagaagtt
    16021 tttcgaaagt atcgagacta cacgatgatt tgcggcggag tcctgtcaga tgatcttgct
    16081 gtacatgcat tgaagaataa agaaaaaata ttggcgcgaa acaaaaagat cattacagag
    16141 aaccttgtga ttttaaaaca atgggtagcc aatgaaccga aagttgaact tgtcgctccc
    16201 aattacgtct ctacttcttt cattaaatta gccatcgaag aagatgacca gacattttgt
    16261 atcaatttat tagaagaaac aggagtacta ttagtaccag gaagtgcctt tgatttaccg
    16321 aagcatgccc gactaggtta ctgttgtaaa aaagaaacct tggaaaaggg attattcctc
    16381 ttatctgagt tcttgaaaaa acaggcctaa acaaaaacac tgcaaggaca gtctgtgaca
    16441 actgttcaag cagtgttttt catttacgct tattgtctat gagaaggaga gtgccctgaa
    16501 gctatggaga ctcctgaagc tttttcttct gcagtttctt gctgatggat ctcttgtgta
    16561 ttgtgtcgta caagcaagaa gttgaccaaa acaagaagag aagtcgaaag aaagacgcca
    16621 cgataaccaa atgcactgga aacactggag ccgatcatag gccctactac gttaccagtt
    16681 gcttggaaag attggttata actgaagatc cgtccagcag cttgatgagg cgaatatttt
    16741 gtgatcaatg cttggacagc agggagcaga caagcatcag agataccaat caaaaaacgt
    16801 aaggcagcta attgccaaac attcgtgacg aatgccattg ggatataaac cagaatcgca
    16861 aaacccaaac caatcgcaag tattctttca ctcccaatct tgtcacctaa gcgaccaaat
    16921 cttggagcgg ctatcaaagt ggcaatccca ggtatcgaag caatgattcc gctgatcaac
    16981 gtaacattcc catgtccatg cagcaattgg cgaatgtaca gactgatgat tggactaatg
    17041 gaattgttgg atgcttggat gatcatcgtt gtgacgaaca taccgatgac gacgtgtgga
    17101 tatttcaagt ctttgaaaat ctgtttggca gaaaccatgt tttctttttt cactgggaca
    17161 aatttttcat ggacgaaaaa cagactcaac aagaagacga aaaataagat gatccctgta
    17221 atgaaaaagg tagggcgata gccaaacgct gaagcagaga tcccgccaag aagcggtcca
    17281 agtaatgttc ctgtaacaga accagttgct aaggttccaa gaacttgacc acttttttct
    17341 tttggcgtcc cagttgcgac aagggcagtg gcattactga tataaccaga aaaaatccct
    17401 tgaagcagac gcaatgcgac taattgatac acactagtca cgcaacccat caaagaaatg
    17461 acgattgcca ttcctaatga agcacgcaac agcatcaatt ttcttccttt ttggtcggct
    17521 aattttcccc accaaggaga gataagcgta gtcaccaaga aagtagatga aaaagtcaat
    17581 ccgctccaaa agttcaattg tgacgtacta taattaccta acgtatctat gtaaagtgac
    17641 ataaaaggca tgactaaact aaatccgata ccagccataa atgttccaaa ccataagaca
    17701 aacaagtttt gttcccaagt ttctcttttc ctaaataatt tttcctttat tcttgcagcc
    17761 aattagactc cttctttctg aaaattttca tctacagctc tttattataa actacaatat
    17821 gtagtttatc aatgaaagtc tcttttttct ctttcttttt cgtggtaaac tgaaagagtg
    17881 aaggatttat tttatgagag gaaacatgga aatgaaaaaa agaaatctgt ctcaagagaa
    17941 aatcatcgac tgctttcgag aactagcaga agagatggat gttcagcaaa taacctttca
    18001 gcatttagcc aaacgcttgg atatcaaggc tccctcgctg tataaccatt tcaaaaatat
    18061 tcgtgatgtc aaaacagcgc taacagcgaa acttctgcag gaacttaatg accagctgcg
    18121 acgggctctt gtgggaaaaa gtggtcagga agcactgcag atctatgcag aaacttatcg
    18181 atcctttgct ttttcaaatc aagcagtgta tgagttactg atcagtgtac cgcataccaa
    18241 tgaagctgtt ttattagatg gaatccatga aacgaatcag atcattttgc agatatttga
    18301 ggcttttcaa ttaacgaaag aagaaaaagt tcatcgaagc agggaacttc gtagtatgat
    18361 ccacggttat ctttccttac gttttttagg atactttaca aaagaaccaa caatcgatcc
    18421 agaagacagc tatttttgga tgatcaataa ctttatcgct actttgcctt aaacaatcag
    18481 attcatttgt aacaatttta agatgttcat taaatgaccc ttttataaga gaaaattctt
    18541 gcaactttct tgaaaagtga ctaagatgag tctagaaaag ctgaaagaag gagcacgagt
    18601 atgtatcaag taattaccat gttcggggac aatgagcctt ggtggttttt tgaagactgg
    18661 gaagaagata ttgaagaaga agaaacattc gagtctttcg aagaagcacg acaagcgtac
    18721 gaaaaacaat ggaacgaaat ccatcaaaat tatgaatata tcaatgcaaa aagtaacttt
    18781 ctcagcgcct tttggaatga cggagatgag cgctggtgtg aagagtgtga cgatgattta
    18841 cagcaataca agggacttgc gttgttgaaa aatcatcagc ccatcacagt agaaagcaga
    18901 aaggaatttt atgaaacaac taattctagc ggaaaaacca agcgttgcga aagacctaag
    18961 caaggtgctt ggtgctaatc aaaagcacaa aaattattat gaaggaccca aggtgatcgt
    19021 tacttgggcc cttggtcatt tattaggatt gaaaatgccg gaagatctga ataaggaatg
    19081 gcagagctgg caaatggaca cgttgccaat gatccctaaa aaactaggaa tcaaaccact
    19141 tcctaaaaca ggacatcaat taaaagcaat caagcagctg gctcagcgaa aagatgtctc
    19201 agaagcagtc attgcgacag atgccggaag agaaggggaa cttgttgctc gctggatctt
    19261 agagtatgtg catttcaata aaccagtgaa gcggttatgg atctcttctc aaacagataa
    19321 agcaatcaaa actggattca agcaactaag acctgcgaaa gaatatgatg cattgtatga
    19381 atctgctctt gcccgggcaa aagcggactg gctggtagga ttgaatgtga ccagagcctt
    19441 aactgtgaag tacgcggata atttatcagc aggccgtgtg cagacaccga cactagcatt
    19501 agtgagagac caagaaaaga aaatcgagca gttccgtcca caaacgtatt tcacgatcac
    19561 gttgcaagct ggaaaagaac aagccaaaat gcttcaaaaa aatcaatttg ctttaaaaac
    19621 acaagaagaa gcacaaaaac ttgttcaatt gatcagtaca agcaaaggaa ctgtcacaga
    19681 agtccaagaa aaattaaaaa cggaatctgc gccattgcct tatgatttaa cagagatcca
    19741 aagagaagcc aatcaacgct atggctattc tgcaaagaaa acattaagtc ttgtacaaag
    19801 tttgtatgag acccataaga tcgttactta tccgcgtaca gacagcaaat atctgacaaa
    19861 tgacatgaaa agtacgatga aagaacgatt gcaagcagtc agcgactttg ctcctgaggt
    19921 caagcagtat ttgaaaaacg gagccatcgt ccaacagaca aaagtattcc aagacagtaa
    19981 agtgaccgat caccatgcac ttttgccaac tgaaaacaga gcacgttatg aaaaattaag
    20041 taatgaagag cgaaaaatct atcaaatgat tgtctctcgt tttttaggac tgtttgctca
    20101 accccataaa gtcagccaaa caaaagttac ggttgaattt ggtaaagaac gattcatttt
    20161 tcggcaaaat cgtgtcgtcc aagcaggatg gaaaggcgaa actgaaactg agacggaaac
    20221 agtcaaatgg gaaaaaggaa tgagaatcaa tcccgatttc gccatcaaaa aagaactatc
    20281 tgcaccgcct aaaccattgt cagaagcaag cttgcttggt caaatggaaa aatacagtct
    20341 gggaacccca gctacgcgtg cagagattat cgagaaacta atcaaatctg aactgatgga
    20401 acgtacccct caaggactgc aagtatctcc taaaggtaag caattattag tattggtaaa
    20461 tccctcttta gtaacaccag atttgacaga aagctgggaa aaagaccttg aagctattgc
    20521 tgcaggaaag aaacaagcag gaaccttctt gaatggaatc gaaaaagaaa caaaacgctt
    20581 ggtgaatgag atcaaatcaa gcaaacaaga atatcaagat ttctcgatta cccaaaagaa
    20641 atgcccagaa tgtggggcca atctgcgcga gaaaaacaca cgtgacggca aaatctatgt
    20701 ctgcaccaat tcagattgca actaccgcag aagaaaagat ccaaaagtct ctaatcatcg
    20761 atgcccgcaa tgtcaccgga aaatggaaat catcgaaggt aaaaacggtg cttatttccg
    20821 ttgtaaatac gatggaacga cagaaaaatt gcttgataaa aaagagcaga agaagaaaat
    20881 gaccaaacat gaagaacgac gcttgctgaa aaaatatgca caagcagaag aaccagaaga
    20941 aagcccatta gcagcagcac taaaagcagc aatgaagaag taataagata tgaaagaggt
    21001 tgtga
//
LOCUS       DACEKH010000045        20817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEKH010000045 DACEKH010000000
VERSION     DACEKH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.046"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(39..1240)
                     /locus_tag="IW662_001530"
                     /pseudo
     CDS             complement(39..1240)
                     /locus_tag="IW662_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1357..1872
                     /locus_tag="IW662_001531"
     CDS             1357..1872
                     /locus_tag="IW662_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0866361.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2071..2517)
                     /locus_tag="IW662_001532"
     CDS             complement(2071..2517)
                     /locus_tag="IW662_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0866362.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2564..4042)
                     /gene="msr"
                     /locus_tag="IW662_001533"
     CDS             complement(2564..4042)
                     /gene="msr"
                     /locus_tag="IW662_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0866363.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4396..5025)
                     /locus_tag="IW662_001534"
     CDS             complement(4396..5025)
                     /locus_tag="IW662_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0866364.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5009..5473)
                     /locus_tag="IW662_001535"
     CDS             complement(5009..5473)
                     /locus_tag="IW662_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0866365.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5960..7228)
                     /locus_tag="IW662_001536"
     CDS             complement(5960..7228)
                     /locus_tag="IW662_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0866366.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7459..8196)
                     /locus_tag="IW662_001537"
     CDS             complement(7459..8196)
                     /locus_tag="IW662_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0866367.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8263..8832)
                     /locus_tag="IW662_001538"
     CDS             complement(8263..8832)
                     /locus_tag="IW662_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0866368.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8960..9832
                     /locus_tag="IW662_001539"
     CDS             8960..9832
                     /locus_tag="IW662_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0866369.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10174..11046
                     /locus_tag="IW662_001540"
     CDS             10174..11046
                     /locus_tag="IW662_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0866370.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11043..13001
                     /locus_tag="IW662_001541"
     CDS             11043..13001
                     /locus_tag="IW662_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0866371.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13252..14103)
                     /locus_tag="IW662_001542"
     CDS             complement(13252..14103)
                     /locus_tag="IW662_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0866372.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14226..14693
                     /locus_tag="IW662_001543"
     CDS             14226..14693
                     /locus_tag="IW662_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0866373.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14956..15438)
                     /locus_tag="IW662_001544"
     CDS             complement(14956..15438)
                     /locus_tag="IW662_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0866374.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15538..16401)
                     /locus_tag="IW662_001545"
     CDS             complement(15538..16401)
                     /locus_tag="IW662_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0866375.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            16999..17847
                     /locus_tag="IW662_001546"
     CDS             16999..17847
                     /locus_tag="IW662_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866376.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(18018..18359)
                     /locus_tag="IW662_001547"
     CDS             complement(18018..18359)
                     /locus_tag="IW662_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ0866377.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            18445..19152
                     /locus_tag="IW662_001548"
     CDS             18445..19152
                     /locus_tag="IW662_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0866378.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            19139..19462
                     /locus_tag="IW662_001549"
     CDS             19139..19462
                     /locus_tag="IW662_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0866379.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(19551..20588)
                     /locus_tag="IW662_001550"
     CDS             complement(19551..20588)
                     /locus_tag="IW662_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0866380.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
BASE COUNT         6202 a         4308 c         3695 g         6612 t
ORIGIN      
        1 acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc atttgtagca
       61 aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc acctgcaaaa
      121 taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa tggtgctaaa
      181 gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg ttgcagacct
      241 ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa aaatcctgct
      301 aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa taacagcaaa
      361 caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa cttccgctca
      421 agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc aaaaaataca
      481 aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc aagtaaagta
      541 aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt atgattgctt
      601 aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt ttcaggtaaa
      661 agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa aaatggataa
      721 cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat tccgcagcca
      781 agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat actgacgatc
      841 gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc acgtaaccca
      901 atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac gatgataaac
      961 aatcctgtca tcaatgtttt ttcaaaccga tccgactaga taagaaacct gtaaatagca
     1021 tcaccacacc agtgactaac atgtaactag taaataacag ggtcgtttca gctggcgtag
     1081 cgtttaactg tgcagcaatc gtttttaaga ttgggtctac tagacccact cccataaaag
     1141 cgatcataca ggtagctgct acaactagcg ctgaactttt cccctttgtt tcttcatttg
     1201 tttgttcttg tatatctatt tgattctcca atatttccat ccctttctct ctattagcat
     1261 catacatgat ttattttaca tgtaaatatg cacatgtaaa acgtggaaac tttactgtga
     1321 tataataaag aaaataagac gagaggtagg atttttatga atactttagc ctatatatta
     1381 ttatgcgtgc ttttatccaa accacgttct gggtatgaat tgaagcagct gatcacaatt
     1441 ttttgggaag cccatcacag tcagatctat acaacgcttg ccaagttaga ccagcagggt
     1501 tatgtgaccg ttttggacaa agacgagcac tctcaaaaaa agatctacca cttgactgat
     1561 gaaggacgtg ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa
     1621 gatgaatttc tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac agctactgga
     1681 ctgctattca ctagagaaca acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg
     1741 aatgacctct cgctagacaa gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga
     1801 attatgcttt gtcagcagga actccaatgg tgtcagcaag taagagaaca aatgcagcag
     1861 ttttttgagt aagggataaa cagaaccttt ctagaaatat ctattgaaga gcaaattatc
     1921 ttcaataaaa gagcaataca gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc
     1981 tatcgttcgg atcactattt ttctagaact gcattcaaga gactatgaca aaaagcatgc
     2041 caaggccttt tttcaaataa cagtgttcca tcattcatta ctttgaattg cttttagcac
     2101 acttcttgaa agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat
     2161 tttccaagca ttcatataag cagagaataa ttgatcaggt attccttgtt ccatgatcac
     2221 accgaataaa gacatacatg tcaccattcc taaaaccatc aagcatgaaa tagttaagat
     2281 catttggacc ttgctttctg gatttactgg tagtttaaag gcaatttttt tcgccagcac
     2341 accgatgatg aagacatcca aaatgaaagc caccacgaat ccaggcacta gaccaatcaa
     2401 caaacttgat aaatgaagat cttgatgcag ccataaatta tacgcgctca tccccaagac
     2461 catcaaaaaa cacatcattg ttgtaaaaat tattccttct tttttatttg taggcaatct
     2521 gtaccactcc tttaaatttg tcacgaaaat catcgtagca catttaaaaa tttctcgtaa
     2581 gtactttttt tattttctgc ccttttattt cataaacttc atcagccgta cgctcgacaa
     2641 agtaagaatc atgagaagta aacaaaatcg ttcccggata tatttgcatt agcttctcta
     2701 aagcttcgat cgttgccata tcaataaaat tagtcggttc atctaacagc aacacattac
     2761 ttggcttagt aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca cttaatgtac
     2821 ataacggttt cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa ctacggacaa
     2881 gcgattctga aaactctgtt tgtctcatga caaaacgaat gatggattct tcagatgtca
     2941 tttgataacc cttttgctga tatacttgaa agcttacttt cggagagaga aggattcctt
     3001 ctccttgttt gcggatatgt tctaataagg ttgtcttacc tgaaccattt tcaccgacaa
     3061 tcgcgatttt tttacctaaa gaaaactgaa aatcaccatt taccagtaat gttctcccac
     3121 ttctttccaa ttggacattt tgtcccatga tgggatagcg gctgtggagt tcaagagatt
     3181 ttggtactgg aaaacggatt tgtttcatct gctgcggctt ggttgtttct cctatttttt
     3241 ggagtcttct ctctaatgtt ttcgcattct tttggatggc tttttgtaca ctgtcttttt
     3301 gtttagagga agacaaccga tccggtctga tttgttgctt tttcttttta ctggacactt
     3361 tttccaactt ttccgcttgt tttttctttt cttggatcga tttttccaat cgttttttct
     3421 ctttcatgat cgactctgct tctcgtagct gagtcttttt ctccaattcc ttttgagtga
     3481 gataggcact ataattcccc gaaaactctc ggatttctcc gtcttttact tcccagattt
     3541 tcgatgctag ctcatctaga aaatagcgat catgactaac aaaaaccagc gtaccataat
     3601 aataacgaag ctcttcaatc agtcgatcaa tactttttct atctagatgg gttgttggct
     3661 cgtctagaag catcccttgt tcataagaag aaagaagacg tatcaactcg atctttcgtt
     3721 cttcccctcc gctccatttc cgctctgaag tttcttccag tcttaactgg aaatgactgg
     3781 ctaattccaa atttaggtcc tttgcttcag cgagataggt taattgaggc aggtagttga
     3841 aggtgatctc tgtttggatt ttcccatgat cgggaaaaag ttcaccggct attagtttga
     3901 gcaaagtcga tttaccgaca ccattttctc ctatgatccc tatacgatct tgctgataga
     3961 cacgaagaga atcaatagat agttctagct ggttgccaaa gctgacttgt agatttgtta
     4021 tatttactgc taaattttcc atatgtttta cactcctatt tcatgtaaaa caacaattat
     4081 cgatactaaa aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta
     4141 ctctgaacga aataactatg actaaatcac caaatctgtc atagctttca cctattctac
     4201 tgtcgttcga aaaaatggac atactccatc catgaaattg ttgtttatac atgtcttttg
     4261 aatttattta gttgttattc aaaagttcga aacgtaattt catcgatgca gtcatttttc
     4321 cgacctcctt ttttattagg ttaccacaca cgattcaaaa aaagcaatag ctactgaata
     4381 gtaactgaaa accttttatt cgaacgtagt gatcttttct ggtgctaaac gaacagactc
     4441 gtaaccttct tctaccgctt ttcctattga taaaataata actggaacat aacggtcttt
     4501 atccaaacca aatgcctctg ccaactgatc tgcttcgaat ccaccaattg gatttgtttc
     4561 atagccatga tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag aatcgacttt
     4621 gactacatcg ttcatttctt ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa
     4681 ttgttgatct ctgacttctt ttggcatctt ccctgattcg taagcttggt tatagatttc
     4741 ttcgccatat tcgtaacatt gcatatcgcc aaaaatcatg accattgcag aagaagtatc
     4801 attttgtctc gtattgaaac ggatcaaagg acgcaagatt tcttttgctt cctcgctttc
     4861 agcgaccaca aagcgccatg gttgcatatt gacagaagaa ggtgcagtcg tcgcttcttt
     4921 gatcatttcc agcatttctt cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga
     4981 tcttctacca gtcataattg ttgaaaaatc atttttttgt tccattatta ttaacctctt
     5041 tcgtttctgt tgttatattg caaagttggg tattcaattt atctaaaagt gttagaaatt
     5101 gtttcatttc tacatcgctg aaagccgggg atagcacgga acctgtatct ttatggtcca
     5161 tttcacaatg ctcgattttc ttctgggctt tttctgttag cgctacatac acttcccggt
     5221 tgttctgagg atttctctga cgcgaaacat agccatttgc ttccaatacc ttcagatgac
     5281 gagtaactgc tccttgatca atctgcaact tttcttgcaa actgctttgt agcaatggat
     5341 tttcttcttt cagtctcatc atgatctcgt atctagtaag acttataccg atattttctt
     5401 caaagatctg gacgattttt tgatcggcta atttgatttg atataaaagg tgactaatct
     5461 cttctaaaga cataatttcc tcctttctaa taattgactc gtcaacgatt gacttgtcaa
     5521 taatagaata aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc
     5581 aatagaaaag tatagagaaa agaaattttt tgtagaatga acggaaaaaa tgataaagaa
     5641 agcacaacgc taaaaccaag attattgatt cttatcaaac tatagaagat agctcttctg
     5701 cagtagaaaa ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg
     5761 aatattagaa attggatcag tatgcttaaa cagtaataaa aaagttatga caagatgttg
     5821 tcacaacctt ttttgagtaa acggtgttca aaaacttgca tctgcggtaa taagttcaac
     5881 caaacaaaaa acatgaaaat ctgtttttgt ttggctgttc ttactacttg atgcagggca
     5941 gctttttcac tacctctttt taataaccat aaaatcctat tccttcatat ctccagccca
     6001 tcggtgttag ccaatctctt tccttagcac tcaaggtata gtgatgggta ccagtttttt
     6061 catttggatt aaatagtcga taaattggct tggaaccacc agaataaaaa cttagacctt
     6121 cataacgcca accgattttt gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat
     6181 ggtctccact gtaaggattg tataaacggt agacaggttg tccgctgttt ggtgcttgcc
     6241 aagcaatccc ttcatattcc cagcctacag ttggcagata atttttttct ccggcattca
     6301 atgtatataa atgttctcct gtatgacggt tatacagacg atacatcgga gaagttgaag
     6361 ttggtgctgt atcatactgt gtcagattat aggtttcgat tacattgttc aatgtagaac
     6421 cataattagg cgcagtggca taacgacctg ttagccaagc cgtagcatct ttgtaagagt
     6481 tcgtattgct tttccatgca ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct
     6541 tcaataccgt tacgttatct tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg
     6601 cattcaccgt aatccattgc ccattcacat attccgaagt aggcatcgta acagattggc
     6661 cattatagct tcctttaatc ccaaatagat tgtaattagg ggcagaagcc aaagtactgt
     6721 tcccccagcc actttcaacg atggcttgag caatcatgac tgatgcatat aagtcattgg
     6781 cacttgccaa actttgtgca gttgaggcaa tactatcaat gaacgcttgt tgagaattag
     6841 cagatgagcg agcataaaaa gcatagccag tatagttttc gaattcagac aaatctgctt
     6901 gatgggtatg tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct tctgcattct
     6961 ctggattgtt ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt
     7021 cagttgttgg ttcttcggtt gaaattgtat tttctgagac agtagctgac tgcaaagtac
     7081 tctctgtatc gttttcaatt cctgtttctg aagaactcga tgtctgagtt gcgacagttt
     7141 ctgtcgattc ttcagctagc actgttgtgt tggcaactgc tagatttcct aaaagaataa
     7201 cgctaaataa taaattcttt gctttcatct tttttctcct ttgaattatg aaacttttcc
     7261 taaataacag tgtaactaaa aaagtaaaag caagtcaatg tccatattta aataacattt
     7321 gtttcttttt gataaatttg ctttttctac ataaaaagac gtaaatgaag aatattcttc
     7381 tccattcacg ccttaaattg atggtcgttt gaataactat ttaacaagta attctctttg
     7441 gatattatcc gttatggatt atacttcctg tgtcgtcgga cggataatca tgttactgac
     7501 agacatccgg tctggcgttt ctatcgcaaa tgctacagca gaagctatat cttcggaggt
     7561 caatccccat tctttttgcg cttcatgaag ttcaagagca gcctttttgt cgctgatggt
     7621 ttgatataat tcagtttgaa cagctcctgg agagatgatc gtagatttga tgttattttg
     7681 ccgttgctct tgacgtaatc cttccatgat tgcacggaca gcaaattttg ttccgcaata
     7741 aacggcggaa tcaggataaa caacatgtcc cgccacagaa tcagttgaaa tgatatgccc
     7801 agatttttgt ttttccatga ttggaagtac ggcggaaatt ccatttaaca cacccatgat
     7861 gttgatatca agcatatttt gccattcatc acggtgacct tcaactaatg gagcggttgg
     7921 catgattcct gcattattgt acaatacatc gattcgaccg aacttttcca tcgctaaatc
     7981 gataactgct tggacttgtg caaaatctct tacatctgct gtttgaatat atagctcagc
     8041 ttctggtaaa gattctttta ttgcttttag gcgatcttct cggcgtgcag ctataactag
     8101 tttagcccct ttttctgcta atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc
     8161 catgatgaca atcacttttt ctgataaatc attcattcta ttctcctcct gttttgaaaa
     8221 atctcttgct tatactcact atctcttatt tcatcttact gttcaaataa tccgttcgac
     8281 aggtttgacg attttggcag ggattcctgc aacgatggta ttgtctggga catctttcgt
     8341 aacagttgca ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg gcagaatagt
     8401 tgctcctgct ccgatccaag catttttctt gatgtggatt ggctttactg acaagcctct
     8461 tcgttctttt ggatcggtta gatggttcac cgtgatgagg ttgacacgag gaccaatcaa
     8521 tactgaatct tctaaacaga tgccacctaa gtcaacgaac atcacttgac gattgataaa
     8581 gacatttttc cctatactaa tatgtttgcc gaaatctgta taaaaaggca gcagaacagt
     8641 caccgtatca tctactttgt cactaatgat attttctagc cgtttgcgga tatctgctgg
     8701 ttcatggtac ccagtattca gcgctgctaa aggctcttcg ttttcagctt ggatctgatg
     8761 gatcacttca aagagcggat ccgttactcg aatctcttta ttgacgattt gtttttcgat
     8821 gaggcttgtc attttgctcc ctccttttat ccttacttta taacggacgg cttatcatgt
     8881 ctaatactta taaaacatac ttaactattc cttttaacta tacttagtgg tatgcttttt
     8941 taaaaacagg agggaaaaaa tggatattcg tatactgacg tattttttag cagtagctag
     9001 agaaaaaaat atcagccgag cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa
     9061 acaactgaaa gatttagaag aagaacttgg tgtgacactt ttcactcggg gctcccgaga
     9121 gatacagttg acggattctg gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt
     9181 ggtagaaaaa acggcgaaca atttaacaaa tgacgcaatc atctctggag aaatctacat
     9241 tggcggaggc gaaactaaag caatctcttt catttctgat agccttcatt cgttgatcaa
     9301 ggcgcatcct gatatccagt ttcatttata tagcgggaat gcagatgata tcatagacaa
     9361 actggatagc ggattacttg attttggact cgtaatcgaa ccgacaaaca aacaaaaata
     9421 ccagtcattt cgactgccag cccaagatcg ttggggactt ttgatacatc gaaatcatcc
     9481 tttagcaaag caaaccgtca tccatccgca ggaactgaaa actgttcctt tgatcatttc
     9541 ccggcaaacg tcagtggaca gtcaactagc tgaatggcta ggtttcagca tagatcagtt
     9601 atcgatcgtc ggaacatata acttattgta caatgcttct ttgatggtcg ataacgggac
     9661 ttttggtgca ttagctctag atgggatcat caatacttat gggacggaaa aaatattcat
     9721 cccactcgat ccgccattac aagcttcact gaatctcatt tggaaaaaag accagcctct
     9781 ttctcctgct gctcgtaaat ttttagatgt cctaacggag aaatacagtt aagtagagtt
     9841 tttttccttc gctttcagct ttttaacaac gaataagata agcaaaagat ccaacgatta
     9901 aataacataa gtttcttaat ttatttcttc cttttatttc tgataattcc agtaaattac
     9961 tataattata aaaaaagaat tgacaaaaca taactgtcta tataaaattg attacgttaa
    10021 caataattcg tttcatgtga ctagatcata ctaggcatga aaaatctctg aggaacatgg
    10081 tttccctagg aggtttttcg tgtctatttt tgtttggccg caatcaactg gtctagcaaa
    10141 cgtgaaaacc aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa
    10201 aatgcagtat tggtagatga cggtggacag gaaaaagtag ctattggaaa agggattgga
    10261 tttgacagaa aaagaaatga tttgattttt gatcgtgatg tcgaacggat atttgtcatg
    10321 gaaccagaag gtcaaatcaa gttgcaaaat ttacttaatc agattgatga acgcttttta
    10381 tttgcagcag aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa
    10441 catgtattga tcgctttgac cgaccacatt gctttttctg cggagaacat taataacggt
    10501 atcgtcatcc gcaacaaact tttaagagaa atcgaagtgc tttacagtga tgaattcagt
    10561 attgctcaat gggcagtaga gtatctgaca aaagagcttg ggattccata tacttatgac
    10621 gaagcaggat atatcgcaat ccatattcac agcggacgaa gcggtcggaa caataaccat
    10681 cgcagtatcc gtgaagtgac gattgtatct gacatcatcc atttagtgga atcagaattg
    10741 gaaacagata tccattcaga accattttca ctgaattatt caagactggt gaatcattta
    10801 cgactgcttc tacaacgaac ccatgcgcaa caatatgctg ttttagacac ggaaatcgta
    10861 gaaatggtca aacgaaaata cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg
    10921 ctgaccaaag aataccagct cgcgatcacg aaagaagaac tgggttactt ggccatccat
    10981 atcgagcgtt taagaagcgc aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa
    11041 aattgaaagc atatctacaa cgaatgggac gttcccttca acttccagtt gcggtcttac
    11101 ctgctgctgc tttactagta gggatcggaa actggtgggc atcttatagt aacgacattg
    11161 tcgctcattt tttacaagct ggcggtaacg cagttttagg tcaattacct ttgctgtttg
    11221 ccgttgggtt agcactaggg atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt
    11281 tggtcgcatt tgaagttcca gtaaatgttt taaaaactga ttcagtagct actttattga
    11341 atatcaaacc agaatcagtt gatccatctt ttgctcagat cagtaacgta tttattggta
    11401 ttattgccgg attgattgcg gcagctcttt ataatcgttt ccacgaagca aaattaccga
    11461 tggctttatc tttctttagc ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt
    11521 tgctgatcag tgcagtgcta ctgctcgtat ggcctccggt ttactctgct ttagtttctt
    11581 ttgctaaatt cattttgagt ttaggctggg gtggtgcagg tctttatggc ttcttcaacc
    11641 gtttactgat ccctacagga ttgcatcaag caatgaactc cgtgttctgg tttgacgtag
    11701 caggaatcaa tgatattggt aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa
    11761 tgtatcaagc tggatttttc ccaatcatga tgttcgggct tccagcagga gcatatgcta
    11821 tttacagaaa tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg ctagcagcag
    11881 cttttgcttc attctttact ggtgtgacag aaccacttga attttcattt atgttcgttg
    11941 cttggccatt atatgtcttg catgcaattt tcactgggat ttctttagca gtgagtgcat
    12001 tcttccattg gacagcagga tttgccttta gtgccggatt tgtcgattac ttcctatctt
    12061 tgaaaaatcc gattgcgaat cagccattga tgttgatcgt ccaaggatta gtaacagcag
    12121 tgatttacta ctttggcttt aactttgcta tcaaaaaatt ccatctgatg actcctggcc
    12181 gcgaggaagc agatctttca gatgacgata ctgcaacgac taattcagac aataaatatg
    12241 cggcacaagc aagcaaaatc tacgctgcat taggcggagc cgacaatgta acttcgatcg
    12301 ataactgtac tacacgcctt cgtttgcaag tcaaagatac aggcctgatc gatcaaaata
    12361 aaatcaaagc cactggcgta cctggtatga agatcatcga cggaaaaaat gcgcaagtca
    12421 tcgtaggtac agaagtacaa ttcgttgcag atgagatggc aaaactacat ggtggtgctg
    12481 cagcacgacc tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc ggtgaaacga
    12541 tcgaacaaga tatttacgct atcgcaaatg ggaaactgat tccaatcact gaagtttcag
    12601 atgatgtatt cgcggaaaaa atgatgggtg acggctatgc tgtattacca gaaaatggag
    12661 aaatcttctc tccaatcgct ggtacgatca caaacatttt cccaacgaaa catgctgtcg
    12721 gaatccaaac agatgctggt atcgaagtct tgcttcatat gggcatcaac actgttgact
    12781 taaaaggcga accattcact ttatatgtag aagaaggtca aaaagtcgca agaggtcaat
    12841 tgattgcttt agttgatctt gccgctattc aatctgcagg caaaaacacg gatatggtcg
    12901 ttgtattcac aaatggcgac aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa
    12961 aagcaaatga taaaatcggc tctgtttcca acaaagcctg agccatcgtc ttccccagac
    13021 ttttttatcg ataaacaaac aggagctgag acaaaaatct tgtctcagct cctttttccg
    13081 aataaaaacg gcgttctcct gccaactccg cggtaataag tccagctaaa cgaaacatat
    13141 gaagagctat tttttatttg actcttctta ttacttgatg cagaacagct ttttcacaaa
    13201 atttttcttc atactcaaaa ctaatcggct ttttcacaac ctctttatac attattcctg
    13261 atccaatatt tcttttaatg ctgcttcaag agaagttaat ggttttccca atactttttc
    13321 taaatcatca gaggaaatag ctaactgatc ctttttaata tcatgttgga tcgctaaaaa
    13381 catgtcagct gcttcttgag ggaatccggc ttcttttaga ttttcgacaa acgctgcatc
    13441 acttgcttca ataatttcta actctttacc tgtcgcacgc tctaatgcct tggctaattc
    13501 ttcatagtca gttagattgc cggataattc taagatttct ggagagtcac tttctactag
    13561 tacttttgct gcagcttctg catattcacg tttcaatgcc caacctgttt ttccatttcc
    13621 tgcagcataa acgaattgac cagtttttaa agcctgccca atgataggta attcattctc
    13681 tacataccaa ttattccgta aaaatgtatg ggaaatacct gaatccttaa tcattttttc
    13741 agtgaattgg tgatctggcg ctaatacact gatcgagtga tcggcatctg caaaacttgt
    13801 ataagcgata taagataccc cagctgcttg tgctgccttc actacattct tatgttcggc
    13861 ttgacgattt cctggaacgc cagagacaaa aagcaatcga tctattcctt tcaacgcttc
    13921 tttcattgag tctaaatcat cataatcacc aatacgtata ttaaatccag cttctttcag
    13981 tgcttctcct ttttcttcac tacgtactag tacatagatt tcattttgtg gaaccatctt
    14041 ttttaagaat tccagcgcat aaccaccaaa acctccagtt gctcctgtta ctaaataatg
    14101 catatttgcc actccctatt ctttttattt gttaagattt acagctgtac atttataaat
    14161 tacaccttta tcataaagag tgtggtaaga taagtcaagc aaaaaaaatg aaaggatgac
    14221 aactgatgaa gtattctctg caatttagtg atgcaatcca tgtacttgcc tatattgaaa
    14281 tgtttcaaga tacccgatca ctttctagtg agatgattgc cagtagtatc gaaacaaatg
    14341 cagcaaatgt acgtaaaatc atgagtcagc tcaaaaaatc cggtttgatc tttacaaaaa
    14401 ccggaaaacc gcagccttcc cttgccaaaa gtccagatca gatttcttta ctagatgtct
    14461 atcgcagcat cgaaggtaat acacagctta tccaagtcga tccaaaaaca aatccaaatt
    14521 gcgtcgttgg tgcgaatatc caagaagctt tagaagaaag ctacgctcgg ctgcagaaaa
    14581 aggcagaaga agaaatggct aagatcacac ttgatagtct gatacataaa atagccgttt
    14641 tggaaaaaga aaaaagacca gaaaacacag gcctcgtaga gagattttta taattctctc
    14701 tactgtgcct gcatttttct gatcggaatc gtctacataa attcttttat tctccggggg
    14761 acaaaagcct tgtcatgccc ctttttcctc tctaatgctc aggtcaaagc gctgaagtca
    14821 tgacgtcttg ataaacggtg ttcaagtagc tgaccctcgg cattagttct gaaaaatgca
    14881 aaatatggaa agctatttcc catttttcct ctctaatgct caggtcaaag cgctactttc
    14941 acgacctcta tattatcatg gtttattgac taccggtatc cagatttctc ccatatattt
    15001 cccgttggta actcccattt cgactacagc atttggaccc ccaacatagg cgtaatcttc
    15061 tacttctctt aaaacatgcc caaaggccgt attcgtcaaa tcatttctta attcttcagg
    15121 cgattctgct aaagcgctta caacaagata cgttcctttt gggaatggga tgattcgcgt
    15181 agtgtttgga acatcctgac tcgtttgaac accgacatag tacatcattt tcccgttata
    15241 gggttcgttg actgtaaaga aataatgatt ttcagcagct tgtttcaatg catctattgt
    15301 tccattaccg ataacttttt gctcaaatgc ttgtttctct gcagccaaac cgacaaaatc
    15361 cgtattgcta ctcttcaatt ccacaccaat acccgtcacc gtaaaatctg gtttttcttc
    15421 gatcctatac tttgccattt ctcccaccct ttctattata ttaagttcat cttacataaa
    15481 attaaaggga aatgacacga ttacgctctt ttttctaggg atatttttat tttttaacta
    15541 tttttttgct ttcactgcaa tccaaatctc actgtaaaca ttactgaaat cctctcccca
    15601 ttttgtaaaa gagatctcgg gagcttcgac taacccataa tctgaagaag gaagccattc
    15661 tgcatagatt ttcccccaag tatcttgtaa agtttgggga aatggccctt tattaggaaa
    15721 aatggcccaa gtgtgttctt ctatagcaag catttctaaa tcagcgtacg gattttcttt
    15781 tgtcgtagca aagccaatca tgtgcatcaa actgcccttt tcttccaagc ggtctccatc
    15841 aaatccataa gaaacgttga ttacttgatt tggatataaa tctcctaact tatgcatctc
    15901 ttccctttga gtctccgtga tcgtctgcgc taacttttgt atttcttgat tgactccttc
    15961 aaatttgaca ggaacttttt tcgccacacc tgccacataa aactgttctt tcttttcgat
    16021 acgaaattcc atcgattttc ctcctctgac atcaataaaa aatgtcaagc gtggaaaaga
    16081 tttttgtatg ccgtatctac tgatttctga aggcagatat cctgaccaat cacgaaaagc
    16141 tcttgaaaaa ccttcgactg tttcatatcc atatctaaac gccgtttctg tcactcttga
    16201 tccatctaga agatcgcgat tagccaaagc caatctacgg tttttgatat agtccattag
    16261 cgacatccct gcaataaacg taaagatgcg ttttaattga tattcagaga ttcctgtatt
    16321 ttcggctatt attttatagg aaagtccttc tgtcaaatgt tcttcgatgt aatcgatcaa
    16381 ttggttcaga tctttaagca tatttatctt cctccttgat tgccttttta tcatattatc
    16441 aaagaaagtc aattacttga tgaatcagga accaatttag tcagatgtag aaaatgttgt
    16501 atggatctaa agaaaattct caagcagcgt ttcgtaacgg ataattggta ttatctgcct
    16561 atctatgttt tacctttaga aaaataaatt ttggggaaga cagattctaa gtaaacaaag
    16621 cggtaaaaat atcttttatt cccttagtta atccgcttac tacctactgt acaaagcaaa
    16681 ttgttgttca aactgctgat cagaacgctg cttggatttt tctttgcgat acatcatacc
    16741 tcttttatca attggaacaa ttatttattt ctaacaaaat cacttcattt ttgtcagata
    16801 aataaaaaac aaaaaaagtt ccatctttaa aacacgctat tcttttactt tttctcacat
    16861 tctctaagaa aagaaaagga gaatgaattt ttgacaattc gagcaaaagt gattatcaaa
    16921 tatgtaaaat cagctacttt tttcttaaac gtttctttat actaaatata aataagtgtt
    16981 tcatgaaagg agaactagat gaataattgg cttgagtatt ttcctgaaaa cgttttggaa
    17041 cgcggttaca gctatcatct gcacggtttt gtccgtcatt tgaactatac aagcaagtac
    17101 ttgtccgcaa cagtttctgg cacggaagat tataaggtag tgatcacttg ggatgaaaag
    17161 accaacatga cttgtgattg tctatatgcc atagaaggaa aaaaatgcaa acacatggct
    17221 gctgtcttat tcgcttacga agaacgtcca ataaaaaaat ccaactactc actttccgaa
    17281 ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac gggagctttt aacagagatt
    17341 cttatagaac atccgcactt tatcgaacgc ttcaaagtta agatgccatt tcatgcaata
    17401 aattattcag ataaattaac aactatcatc cataaatacg atcatattat caagaaaaac
    17461 aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa agttcatcca agaagcagtt
    17521 gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg aattgatcaa tgaagtgatt
    17581 gctactctcg aaacattcta ttgggagcct gaggatgaac gaacccttct tctgatagag
    17641 gactgttatt atttatggaa agaattatta gcagaagcac ctcatgcaga aaaaaggcaa
    17701 atgttctctt ggttcgtctg ccaagtcgat catactgacg cctcttattc aaaacgctac
    17761 tctataaaaa tcctgaaaga agactttcgg gaaaaagaat tttctaacca aaagaaaaaa
    17821 atagataaaa aactaaagaa acggtaaaga aagacgattt caatgaaaaa tagcaagtaa
    17881 acgataacaa ccatcctaaa tccacaagaa atacagcaga ccttgcttta aaaacacgaa
    17941 tcatgcaaag gatgaagtaa agaagactta acgcgtaccc aatattagtc atttgactat
    18001 taggtacgcg ttttgtttta taagtcatga caaatttcct tcgttgctgg atagattttc
    18061 cggcattgtt gatagatttt atgatacttt tctacatttt gtaagagagg ttccgctgca
    18121 tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg tgtcgaacca ccctaaccct
    18181 agtgcagcaa tcattgttgc gcctaatgca ggtccttcct ccatacttaa acaatagacc
    18241 gttgcatcga aaatatcagc ttggatttgt agccatgcaa gatttttcgc gcctcctccc
    18301 accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt tacgtttcaa tcccaccatt
    18361 gacaaatgct cattaaaaat ttagaatatt cacaagttta acgacaatta tgaaaattta
    18421 tgtcgtgcta aaggagtttt gttattggat gaaaagttag atataaaaac tgcgatcaaa
    18481 gacacgttac ctaccgtttt cggttatatc ggtattggac ttgcatttgg tatcgttggg
    18541 aaagctgccg gatttcatcc actagtcgtc acgttgatgt ccctgctggt ctatgctggt
    18601 tctgcccaat ttatcacagt cagcatgctt gctagtcaca gcccattgct ttccatcgtt
    18661 ttctcgacct ttctagtcaa ttcccgaatg attctgatga gtatgacgat tgctccttat
    18721 ttcaagaaaa atcggctgct tcaaaatctg ctgattggca cattgctgac agatgaaagt
    18781 ttcgcactgg gaatgaataa attgaactat actggacaaa agttaaactt tcgttggatg
    18841 aatacagcta attggatttc ttatttgacg tgggtgggtt cttctcttgt tggcgcactt
    18901 ttagggaatt ttattactga tccgaaaaag tttggtttgg atttcgccat cgtcgctatg
    18961 tttatcgggt tactttatct tcaagtcatt tctgatagaa acacctctaa acgattacag
    19021 ttgatcctga ttggtctcac tttgattctg gtatatgtcg gactgatttt tatcccaagc
    19081 aacttagtga ttgtagttgt aacactgatt gggtgcggat tgggggtgtg gatcaaacat
    19141 gccttctttt gagtttacat tgctgactat tttaggttgt actttagcaa cttggctttc
    19201 tagggtccta ccatttgtct tactgaaaaa attcgattta ccgcaaccgt tgcttgagta
    19261 tttaagtttt gtcccgatcg tcattatgtc tgctttatgg ttcagcagtc tatttaccca
    19321 aaatatcgga catctgccgc agatcaatct cgaaaatgct ttggcttcag ttcctacact
    19381 tttagctgct attctttcaa aaagcttatt ggtgatcgtc ttggcaggaa ttctttccct
    19441 ttcgttgatt cgcttattat aaataaaaaa acaagagctt atggcaaatt actgtcatgc
    19501 gctcttgttt ttatcaaagt caaataagaa aaaaatcggc gtattcttgc ttatccgttg
    19561 aacggaccac taacaacgac ttttcctaac atatgtcctg tttctacgtc tttggttgct
    19621 tgtttcaagt tcttcgcatt catcccatct gaatagacct tgctcaatgt actgcggata
    19681 atcccttcat ttgctaaagc agcaatgtgc gctaaagctt ctccttgcgt ttcgatttca
    19741 tagttatagt ctgttttagc gaacatatac tcccagtcga agctgataga ttggtttttc
    19801 caaacaccta tatctaacgg ctctttgatc cctacgatcg ttcccacatg tccaaatggt
    19861 cggacaaggt gcttgatttg atcaaagtag cgggtaatat cgaataatac agcgacatac
    19921 tctacttgat cgattcctaa ttcacgaaaa cttttactta agtcgtgatg ataatcgatt
    19981 ggatagtcta cacctgtttt tttcagccat tcaaaattct tcggacttgc agtcgcgtag
    20041 acttctaggc cagcccaatg cgctagttga ttcaaaatcg aaccgacgcc cccggatccg
    20101 ttgatcacta agattttttt cccgcgattg gcattttctt ctggagttag accaaatttt
    20161 tcaaataaca attcataagc ggtcaatgag gtcaacggca aggctgcagc ttcttcatct
    20221 gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa ctagttggta ttcttgattg
    20281 cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat cgccgacaga aaatttttgc
    20341 acttcgtcac ctacagcaac aactttcccg actccatcaa aacctaagat acgtaaagca
    20401 tttcttatcc cgttatcttg gcgaagtttc gtatcgacag gattaacaga tactgcgttg
    20461 acttttacca gaagatcccg tgaactgggt acaggtatct gttctttttc atcaataaag
    20521 ctttttggat catcgatcgg caacccttca taaaaaccga ctaccttcat taattccttt
    20581 tctgacatac gaattcctcc ttagcttcat caagttggag catccataca gtgttcattt
    20641 caatatatat agtatgctat ttgttccacc ttttttattc atcataatta ttctcttact
    20701 taaagtaaac tatttgcact tttttggtta aaaaagaggt tgcgacaaga cttttgtcac
    20761 aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc acaagtcaaa atattca
//
LOCUS       DACEKH010000046        20801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEKH010000046 DACEKH010000000
VERSION     DACEKH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.047"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..300
                     /locus_tag="IW662_001551"
     CDS             <1..300
                     /locus_tag="IW662_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0866381.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(724..894)
                     /locus_tag="IW662_001552"
     CDS             complement(724..894)
                     /locus_tag="IW662_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0866382.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1088..1267)
                     /locus_tag="IW662_001553"
     CDS             complement(1088..1267)
                     /locus_tag="IW662_001553"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866383.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1277..1501)
                     /locus_tag="IW662_001554"
     CDS             complement(1277..1501)
                     /locus_tag="IW662_001554"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866384.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            1654..2766
                     /locus_tag="IW662_001555"
     CDS             1654..2766
                     /locus_tag="IW662_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0866385.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2844..3137)
                     /locus_tag="IW662_001556"
     CDS             complement(2844..3137)
                     /locus_tag="IW662_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ0866386.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3305..3619
                     /locus_tag="IW662_001557"
     CDS             3305..3619
                     /locus_tag="IW662_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ0866387.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3640..5619
                     /locus_tag="IW662_001558"
     CDS             3640..5619
                     /locus_tag="IW662_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0866388.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5632..5958
                     /locus_tag="IW662_001559"
     CDS             5632..5958
                     /locus_tag="IW662_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ0866389.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5974..6492
                     /locus_tag="IW662_001560"
     CDS             5974..6492
                     /locus_tag="IW662_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866390.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6492..7844
                     /gene="celB"
                     /locus_tag="IW662_001561"
     CDS             6492..7844
                     /gene="celB"
                     /locus_tag="IW662_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0866391.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7928..9433)
                     /locus_tag="IW662_001562"
     CDS             complement(7928..9433)
                     /locus_tag="IW662_001562"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ0866392.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9525..10115)
                     /locus_tag="IW662_001563"
     CDS             complement(9525..10115)
                     /locus_tag="IW662_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0866393.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10294..11940
                     /locus_tag="IW662_001564"
     CDS             10294..11940
                     /locus_tag="IW662_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0866394.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11989..12708
                     /locus_tag="IW662_001565"
     CDS             11989..12708
                     /locus_tag="IW662_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0866395.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13187..14134
                     /locus_tag="IW662_001566"
     CDS             13187..14134
                     /locus_tag="IW662_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0866396.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14146..15066
                     /locus_tag="IW662_001567"
     CDS             14146..15066
                     /locus_tag="IW662_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0866397.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15107..16564
                     /locus_tag="IW662_001568"
     CDS             15107..16564
                     /locus_tag="IW662_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0866398.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16909..17523
                     /locus_tag="IW662_001569"
     CDS             16909..17523
                     /locus_tag="IW662_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0866399.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17516..19240
                     /locus_tag="IW662_001570"
     CDS             17516..19240
                     /locus_tag="IW662_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0866400.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19237..20688
                     /locus_tag="IW662_001571"
     CDS             19237..20688
                     /locus_tag="IW662_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0866401.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6709 a         3510 c         4166 g         6416 t
ORIGIN      
        1 gatggcttag caccaggagc atatcagctg gtagaaactc aagcgccaac ggggtatgaa
       61 ttggatgcga caccagtaac tttcaagatc gaaaaagaac aagaagtggc gatcttttta
      121 acaaaagaga atagaaaagt tgctgatagc tcagatataa gaggaaacaa tccaacaagc
      181 agtggaaata agcatcttcc aaaaactgga gaaaccatca ttagccagtt tatattgtca
      241 atattgggtg tgctacttgt tttcatcagt ataaaattta gaaagaaacg aaatatatga
      301 gctaaaataa aaaattgaat aagcatagtt tttaaaatca actagtaccc atcaattgaa
      361 tggaaactac cgattttaga aggggtatac aaacaaatat tagtatcgga gtagagaagg
      421 ttgaacccaa ccagtaaata agtaaagaca attttgtgct gcgttgagaa gtgaaaaata
      481 aacctttgta gtaatactag ttaataaaaa atatacaaaa acactgcgaa aatcattctt
      541 gagtttcgca gtgttttttg cctaatgact caagtttatt gggtaatact ttgattttct
      601 ttctacaaat aatcagcgat cagttgtaat tttttctcta gttttaatgg ctctttgtca
      661 ataaggagaa aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa
      721 aggttacgcc tccttgaaag gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc
      781 atttggtaga acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa
      841 ttctatttct ttaggattag tgcagctaag gaaatagttt gttaatcgag gaattttttg
      901 tgtaggtaaa ttttctcgat taatttttct gtctcgccat ccgcggaaat aaaactcagt
      961 ttatcctcaa cggaaacgtg agaacgaaat tcacaaccct caacactcaa gacagaattc
     1021 ttttggaaga ctttggtaaa tagcttctca actctttcaa tgtgcgaata acggttgtca
     1081 aagataccta acaacttttc gaaataaaaa ataacgacac tttttcagtc agtaaagaag
     1141 tgattttatg tctgacatga tttgaaatcg agtgccttgg acggataaaa taactttgag
     1201 cgttccaatg gcttcgacag tttttacagg tatagcgctg cttttttagg cgtataacca
     1261 ttggcatatg attatattat tcaaagtgga caatggtttc cttcttcttg tttttcacca
     1321 ccattaattt cctatttcca tctaccacag cagaaccaca atttctacag acacatggag
     1381 cagggaagag aactgcagca acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga
     1441 cctctgtaat catcaaattt ttgtctattg atctcagaat ttttttgata gaaccattca
     1501 taaagcatat cgttctctca gttgtttatt ttgtggtgat ttaatcatac aagaaaacga
     1561 tatgtttttt tataaaaaca tgaataggaa aaatagttga caagaaaatg acaacgccat
     1621 atgatgttcc atagtattta caacctgtca tagatgactg taaaaaataa aagtaaaaag
     1681 aaaggacgac aacaggtgga tttttatcag tctctacaac tagatccgtt tattttaaaa
     1741 caaaaaatac gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt
     1801 ttacgatcat tattcatcgt attgtttgcc atttgtttta tcattataat tacaacatta
     1861 tttgaatcga agcataaacc atacgctgtg gttttattct gtatgttgat gagtattcgt
     1921 tttgttgact ttggttataa gatttcccat tcgatcatag gtttagcaat cgtcatgttc
     1981 tcactattag tagcaccgta tgtccagctg atcaaatggt cagttatggg catgttgatt
     2041 cattttattt tactaagcag catattaatg gcaaccgcta gtgatcccaa aatggggaat
     2101 gcttctttgt acggcttttc atatctcttt attgtttatt cgttacctaa agattcgttg
     2161 aacaagaatt tctttacaca aacaagtagc ttattatttt tgtttttttg ttggttttca
     2221 gttctccttt acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt taaagagatt
     2281 tttttgaaag gcatgtattc gcaggaaaaa atttggatgc tcatctatgc atttggcatt
     2341 agtcttttga tcgttgcagg agagtatgtc ccttttcagc gattgatgtg ggcagggttt
     2401 gcattttctt ctattgtatc tagctatgga ttgatgtcga ttggtttcaa agaacgagca
     2461 gtagaccgaa tcattggctc attgattgga tgtgttttat ttattggtat cagccaattt
     2521 attcctttta actgggtcgg tatattaggt ggtctagctt taggtgtttg ttctacctat
     2581 agatataaaa cagtttttaa ttcttttggt gctttggcaa tcactgccag tttattcggg
     2641 gttccaggag cagtaacgat ccgcattttt gagaatatcc ttggggtatg tttaggaatc
     2701 atgtatattg gggtgactga aatacttatc cgaaaaataa gagaaaaaca cggtttgaat
     2761 cattagatga gatcgaataa aaaataagga aaaagtgaag atacacatgg atgataaatt
     2821 gctatcattc catgtgtatc tttttacggt aaatcagtga aacgttcgac tttcattttt
     2881 aatttatact ttttacggag ttcgtttatt tgagccatca tttcggattt gtgatgcttt
     2941 tcgattgctt cttcatcttt ccaacggtcg atcagtaaaa gagattctgg atcttctagt
     3001 ggaaagtaat agtcatattt ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt
     3061 ctttcgacca tttcttctgc aaattttctg gcttgtccat ttgtacttgt atacaaaata
     3121 ttcactgtga tactcactaa atcccctcct gaaaaaattc tatcactagt atatcacggt
     3181 gccagaaatt attattcaag atatgataca cattttttcc gcaatgctac ggaaatatat
     3241 attttgaata gaaaacgctt acaagatatg cttggtttat aaaaataaaa ggagtgattg
     3301 atttatgaaa aaagcattga ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc
     3361 aaagacaacg gatttcttta agcaaaaaag agaagaggta ctcgtagatg cagtttcagc
     3421 tacagaaggc gataatatga ttaagaatag tgattttgat ttatttctga tcagcccgca
     3481 aacgacgatg tttttggata aattcaaaaa attaggagaa tctgttggaa agcctgttgt
     3541 tagtatccct ttccaagcgt atgtcccaat ccctacaggc atccagaagt tagcagaggt
     3601 gatcgaagag aacatatgat ggaataggag tggatgataa tgaaacagaa ggaaaaattg
     3661 ctgttgagat atttgattga tcacaagaag gagtatgtca ccagtcaacg tttagcttct
     3721 gaattgttat tatctgatcg gacaatccgt aattacttgc agcggatcaa agagttagta
     3781 gaaaaaaacg gaggtaagat cattgctaaa ccaggttatg gctaccaact gcatatttta
     3841 caaagactga catttgactt gtttttgagt cggcaagaaa tagtacctgg atatgctaga
     3901 gaaccgcaag gattttatga gtccgaagat cgacaaaagt atatattgaa ccaattgctt
     3961 ttggaagatg ccattctttt tatggatgat ttggcagaag agctttatat cagtcgttcc
     4021 agtttgacaa aggacatgca agaaatcaag gagcgattga ttccatattc actaaaaatc
     4081 gtttctaagc atggtcaagg gacatggatc gaaggggaag aaagaaatcg acggcatttt
     4141 atcatggata ctttttttgg aaaccattat ggaaattcat tgaaagaata tttagggcaa
     4201 agccgttttt tcccagacat cagttttgaa gagctagtca ttatcattct cgatgaaaca
     4261 agagaagcta agttaaaagt ttcggatttt atcatacaga atctgacttt acacttggct
     4321 ttggggatca aacggctccg agaagggttt gaaatcaagg aattaggcat cgaacaagaa
     4381 gtttctaagc gagtagagta tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc
     4441 gcaggtgtat cgtttcccga agaagaaatt gcttatttga cattacatct catggctaag
     4501 tcgaatcaca aagaaaatca aaatgatcag gaactatcaa aggaattagc gaaagttctg
     4561 aaaagattgt ctcaggaatt tcatttttca ttagtagagg atctccagct tttcaatgga
     4621 ctactcgatc atttgtcacc gatgatgatt cgattgagcc gaggtattcg cattgagaac
     4681 ccattgactg aggagataaa gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat
     4741 ttcagcaaca tgccggcttt gaaaaactat accatcaatg aagatgaatg ggcctatttg
     4801 gctttgcatc tcatggcggc attggaaaaa gaaagagctg cgcataaact acatgcattg
     4861 attatctgtg caacgggtta cggcagtgcc caactattaa agaatcgcgt agtcagtgaa
     4921 ttcggtaaaa atattacagt ggtaagtgta aaaggctatt atgagatcaa tgaaacaact
     4981 ttaaaaggaa tcgacctgat tatttcttct attgatcttt cgacaatgtt tttcaaaatt
     5041 ccagttttac atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta
     5101 gttggagaat ctatacccta tcattcctca gatagaccga taccagcatt ttctctacag
     5161 caaaaaaagc agatttacac cgaacagttg tctgaaaaat ttttcaagac ttatacgtac
     5221 gaaccgaaaa aggaagaaat tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa
     5281 gaaggatata taagagaaat gcgaaagcag atccagcaac gtgaaaatat gggacaaatc
     5341 atattcagtg atacagtggc agtcccacat cccgctgttc cagttggaat cgaaacaaag
     5401 gtagccgttg cattgattcc ttctgggatg cagtgggatc aatatcaaga aattcatttt
     5461 gtttttttag tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca
     5521 atcgttaggt tagttgacca gctaaaagta cagcaggaaa tcttagctga acctattttt
     5581 gagaatttca gcagtaaatt tatcaaactg atcgaataag gaggaaaaca gatggctgaa
     5641 aaaataaaca gtgaaacact acaagtgact tcttttgata tcattttcca tagcggaaat
     5701 gcacggacac tgatacatga agcatttcgc tcgatgcgta aagagatgtt tgaagaagta
     5761 gaagagaagt tgcgacaagc aaatgaagag attcttcatg cgcatcagtc acaaactcaa
     5821 cttctgaaag aatatgccag cgggacaaaa atagaaatgg aaattatcat ggtccatgca
     5881 caagatcatc tgatgacgac gatgacatta ttggaagttg cacaggaaat gaattatttg
     5941 tatcaaaaaa ataaatagaa aggaaggatc attgtgcagg atacagtaaa aaggaaaatc
     6001 gaattagaaa aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta
     6061 cgttacctga ctgccttttt ctttttcatg aacatgcaat ggatgattct gcttctatca
     6121 gctaaaagtt taggaagtag tttaccgatg attctgattt tagctatttt gcctgcagta
     6181 ggagaacaag taaagcttta tcgaaaacac caaacgaacg ttccatggac aaagtggtat
     6241 tttttgttcc agagtgtctt taatttatta gtgattccag tgttgtttac ctcaggattt
     6301 acattcttgt atccttttat ggcaaataac aatggaggac agttatttgt tttcattctt
     6361 gttgtaagcg gtatttttgc cagtgcattt atacagtatc gtttaaagaa aatttcactc
     6421 aaccaagacc aacagtatat ccgtattaaa caatatgaaa aagcgttata tttaggaaag
     6481 gagaataact aatgtttgat tttttgcaga aatacctgat gggacctatg gggaaaattg
     6541 cgcaacttaa aatcgttcgg gctgtcatgg cagcggggat ggcttctatt cctttcacga
     6601 ttgtaggctc gatgtttttg gttttaaata ttttgccatt accgttccca tttttagaag
     6661 gattttttaa tgcaactttt ttcaaagtta gtgacttgta catgatcgtc aatacaatga
     6721 cgatggggat tttatctatt tactttgcca ttgtgtttgc ttatgaatta acgtcaattg
     6781 aacgagatga acaaaaatta aatgttaatc cgctcacggg ggcattacta tctgtttttg
     6841 cattctttat gtgtatccct gaattgatca tgactgatgg taaaatcagc ttgattgctt
     6901 ctatcaatga gacagaaact gtagtaaatg gtattaggat gggctctttt gtcgaacgtt
     6961 taggaacttc aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt
     7021 gtatgtgtgt caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt
     7081 ctcgttcttt cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca
     7141 atggattatt agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt
     7201 ttgttacgca gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg
     7261 cattgtggat cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg
     7321 tattagctaa tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca
     7381 acaattctta tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg
     7441 cattcctcgc gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat
     7501 tattcaatat caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag
     7561 cggttccgtt tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc
     7621 tggaaatcgt caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag
     7681 catttgttgg tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca
     7741 tcgcattcat tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag
     7801 aaagagaaaa ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg
     7861 aactgtgaca aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata
     7921 aataccatta ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat
     7981 ctacaccatt gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt
     8041 gcggagcgct ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa
     8101 cgatcaaatc tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt
     8161 caaatgctgg gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc
     8221 caaaatcatg tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac
     8281 ggccatctgg atgttcattt ttggcaaatt tcagcaatgt tttcaagctt agataattat
     8341 gtgcatagtc cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg
     8401 gtacacgagc ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg
     8461 cggcaatgat cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat
     8521 aagcagtatc gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac
     8581 ctttatctga acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg
     8641 gaatctggaa tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta
     8701 aaacaactgt ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg
     8761 tcggatgttc gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta
     8821 aagtcaagcc gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat
     8881 gagtcatacc attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg
     8941 ttgtcaaatg ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca
     9001 atgctgtttt tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg
     9061 ttttaccttt tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa
     9121 aagccatagc aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg
     9181 aattttcgta tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca
     9241 aatattcttc tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc
     9301 ttgaatcata acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc
     9361 ttgttgaaac aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta
     9421 aagagaagct cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac
     9481 tctttttaat atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt
     9541 gacacagggg acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg
     9601 agaacgaata gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta
     9661 ttggtaagta aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc
     9721 ggtatcgtta taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg
     9781 aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga
     9841 acaatctcta aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat
     9901 tttcctaata ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact
     9961 gagcgttgga tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa
    10021 gctatatcgt gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa
    10081 aatataacgg gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca
    10141 taaaaggctg tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat
    10201 tgttcttata aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat
    10261 agtaatgttt tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta
    10321 gatcaattaa caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat
    10381 atcatttcaa gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa
    10441 aatgctaagg ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta
    10501 ctgatctcta cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca
    10561 ttgaatgaat atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg
    10621 ttaggcctgt tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct
    10681 ggtggcggag aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt
    10741 ccttgtatga gtgtgatacg agggtatttg caaggcaatc aagaaatgat gccttatgcg
    10801 ctgtcgcaga ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc
    10861 atcatgaaag tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca
    10921 tttatcggta tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg
    10981 tatacatcgg cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg
    11041 ttgttagata ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc
    11101 ttcaaattag tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc
    11161 aatgcacagt tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg
    11221 gtagttattg cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct
    11281 gttacgttga aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt
    11341 tcatttttta tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca
    11401 ttattttata caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga
    11461 ctcttcctag gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag
    11521 gcggcaatta attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc
    11581 attcgtttat ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct
    11641 tgtaccttga tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg
    11701 cgcaggacat tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca
    11761 aagatgattt ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt
    11821 cttgtagtag caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg
    11881 gcagaaagac tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa
    11941 gtgaatcaga agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat
    12001 aagtttttag cagatatggg acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa
    12061 ggaacgatcg aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc
    12121 gaagatgcgg ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt
    12181 ttgcataaac ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc
    12241 gatttattgg aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa
    12301 gacacagagg gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca
    12361 cctaaaaaac atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa
    12421 gatatccgtt tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca
    12481 aaactccaaa ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg
    12541 catgaaggaa agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta
    12601 tatttaaaaa gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa
    12661 tatcgaccca tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag
    12721 ctaaaagaat aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact
    12781 ttcggatggc ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc
    12841 gtttgttcgg aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg
    12901 actcagagtt agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc
    12961 acaacctctg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    13021 aaatatgagg aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc
    13081 acaacctctc ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt
    13141 gaaagcgcta taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa
    13201 ggggttcaat cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt
    13261 accgggtatg ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc
    13321 attcaaaaat tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg
    13381 ggtgggttgg gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa
    13441 aaatacaatt ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt
    13501 tgcaatcatt atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc
    13561 cttactacct tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag
    13621 tcctgacaaa ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg
    13681 gtataatgaa ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat
    13741 tggttataac agcattattt actttgcatc tgttatggga atcgatccga cttattatga
    13801 agctgctatg gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca
    13861 acttattcca ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga
    13921 ttttggcctg ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt
    13981 tttggatacg tatatctaca atggcctgac atccacagga gattttggta tgacggctgc
    14041 tgcaggactt taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc
    14101 ccgacgtttt gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa
    14161 gtggagagag tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg
    14221 attgcactct ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt
    14281 accgatgagt ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata
    14341 tttggttata cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc
    14401 actgtacttg ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct
    14461 tatgcgattt cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt
    14521 acgatgttgt tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag
    14581 ttaaaagata cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt
    14641 gtgatgcgca cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc
    14701 gatggagcta gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt
    14761 attgcgacaa tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta
    14821 ctatacatcc aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt
    14881 aatattcaat atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc
    14941 tcagttccag gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg
    15001 atcagctatc catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa
    15061 gaataaagaa acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa
    15121 gaaactagcc tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg
    15181 ttctggttct aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat
    15241 cggagataaa ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa
    15301 agagatcggt gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat
    15361 gaacgtcatc atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc
    15421 caatgcccaa aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa
    15481 agcttataat gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata
    15541 tgctttccca gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt
    15601 actagataaa tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt
    15661 cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt
    15721 caaagtccaa gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct
    15781 ggatggagat gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt
    15841 acgtacaatg catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa
    15901 taaggattat ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta
    15961 tgattatggc gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat
    16021 tactgttcca ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac
    16081 ttcaaaaaat aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt
    16141 attgaatggg cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga
    16201 tgataaaatc aaattgctag atggttatca gccaaataca catatgtctg cttggaatac
    16261 aggaaacaac aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg
    16321 tgatcaatcg attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga
    16381 cagtgtcaaa acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt
    16441 gaatacaggg actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa
    16501 ggctggctat gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga
    16561 ataataatga ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg
    16621 aataaacggt gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg
    16681 aaagccatat tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc
    16741 ttggtaaacg gtgttcaatc agctaaccct cggtattcgc tcacaaaacg acaaaatatg
    16801 aggaactatt tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct
    16861 ttttttgtat aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag
    16921 gttttggaag agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg
    16981 atatatacat ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat
    17041 gaactatttt atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat
    17101 atatataaaa gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact
    17161 gctttattga gttacaattt atatttatct gttcaaatca aagggctcat ttttttagct
    17221 attgatttta ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg
    17281 attttagata gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg
    17341 gttttttcca gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc
    17401 gtgacttggc aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg
    17461 aatggtacga tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa
    17521 taagtttcgg attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc
    17581 ggttatcatg atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga
    17641 acaaaatcaa aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat
    17701 gctgaatgat aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga
    17761 aaagatcgaa aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt
    17821 atacgaacag caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct
    17881 ctacgcaaat tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta
    17941 ttatgaatct tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact
    18001 agacgccttt tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt
    18061 tattggtttt caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg
    18121 actctcttta tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa
    18181 aatagctaaa gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa
    18241 ttcacaattg ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc
    18301 tgatgattat cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa
    18361 aaatcttgcc ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct
    18421 ttataaaaca ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca
    18481 aacagcagca gggaatttag agacgcggat cgacataaca aacacggaat atgaactgaa
    18541 ggaactttca atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat
    18601 ctacaaatta gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa
    18661 tccacacttt ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag
    18721 tgaagaatta gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca
    18781 agagaaaacg atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta
    18841 tcaaatgcgt tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa
    18901 gatcgaagta cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat
    18961 tgattttact cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt
    19021 aaggatcatc atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga
    19081 agaaaaactg tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa
    19141 tgaacgttta cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac
    19201 aggtggactg accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg
    19261 gtagatgatg aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta
    19321 ggctttcaaa tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag
    19381 cgacagattg atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt
    19441 attgaagcgg ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa
    19501 gaattcgatt atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc
    19561 gtgaataagt tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa
    19621 aatgaacaaa aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat
    19681 gaagaattgg atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat
    19741 aggatagtat tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa
    19801 gaacggcaac aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg
    19861 ttgttggagg cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat
    19921 caggaatggc tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca
    19981 ttccaacaag cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat
    20041 gttttttacg cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga
    20101 cgcgtgctgc aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa
    20161 cagtttcagt tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt
    20221 atggagattc aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa
    20281 aaaatccatc aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa
    20341 aagcatcaga gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac
    20401 cagcattatc aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc
    20461 atgtatctag gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac
    20521 catttacgta tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag
    20581 atagcaagtg agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc
    20641 gtcggcagat cccctaaaga gtaccgagaa aacagtggag aatcataaaa gaaaaagcca
    20701 atgtaatgaa ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta
    20761 cttgaggcaa ggcagctttt tcatgacctc tcgataaacg g
//
LOCUS       DACEKH010000047        20522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEKH010000047 DACEKH010000000
VERSION     DACEKH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.048"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            292..1431
                     /locus_tag="IW662_001572"
     CDS             292..1431
                     /locus_tag="IW662_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ0866402.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="IW662_001573"
     CDS             complement(1498..2073)
                     /locus_tag="IW662_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0866403.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="IW662_001574"
     CDS             complement(2078..3052)
                     /locus_tag="IW662_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ0866404.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="IW662_001575"
     CDS             3335..4303
                     /locus_tag="IW662_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0866405.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="IW662_001576"
     CDS             4416..4694
                     /locus_tag="IW662_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0866406.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="IW662_001577"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="IW662_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ0866407.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5160..5906
                     /locus_tag="IW662_001578"
     CDS             5160..5906
                     /locus_tag="IW662_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0866408.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="IW662_001579"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="IW662_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ0866409.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="IW662_001580"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="IW662_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ0866410.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="IW662_001581"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="IW662_001581"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ0866411.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="IW662_001582"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="IW662_001582"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ0866412.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="IW662_001583"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="IW662_001583"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ0866413.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="IW662_001584"
     CDS             10643..11185
                     /locus_tag="IW662_001584"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ0866414.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="IW662_001585"
     CDS             11208..12764
                     /locus_tag="IW662_001585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ0866415.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="IW662_001586"
     CDS             12778..13680
                     /locus_tag="IW662_001586"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ0866416.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="IW662_001587"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="IW662_001587"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0866417.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="IW662_001588"
     CDS             15123..15545
                     /locus_tag="IW662_001588"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ0866418.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="IW662_001589"
     CDS             16014..16244
                     /locus_tag="IW662_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ0866419.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="IW662_001590"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="IW662_001590"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0866420.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="IW662_001591"
     CDS             17655..17831
                     /locus_tag="IW662_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0866421.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="IW662_001592"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="IW662_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ0866422.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="IW662_001593"
     CDS             18326..18709
                     /locus_tag="IW662_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0866423.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="IW662_001594"
     CDS             18878..19762
                     /locus_tag="IW662_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0866424.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19879..20112
                     /locus_tag="IW662_001595"
     CDS             19879..20112
                     /locus_tag="IW662_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ0866425.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="IW662_001596"
     tRNA            20200..20280
                     /locus_tag="IW662_001596"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="IW662_001597"
     tRNA            20287..20358
                     /locus_tag="IW662_001597"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
     gene            20404..20474
                     /locus_tag="IW662_001598"
     tRNA            20404..20474
                     /locus_tag="IW662_001598"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20436..20438,aa:Cys,seq:gca)
BASE COUNT         6804 a         3598 c         4461 g         5659 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagtt tctataattt attctttatt ttatagattg ataatagtat
    20401 aatggcggta tagccaagtg gtaaggcaga ggtctgcaaa accttcatca ccggttcaaa
    20461 tccggttacc gccttagtac taataaatca acttgccggc gtggcggaat tggcagacgc
    20521 gc
//
LOCUS       DACEKH010000048        20508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEKH010000048 DACEKH010000000
VERSION     DACEKH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.049"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(2..1990)
                     /gene="treP"
                     /locus_tag="IW662_001599"
     CDS             complement(2..1990)
                     /gene="treP"
                     /locus_tag="IW662_001599"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ0866426.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2213..2926
                     /gene="treR"
                     /locus_tag="IW662_001600"
     CDS             2213..2926
                     /gene="treR"
                     /locus_tag="IW662_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ0866427.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3003..3449
                     /gene="msrB"
                     /locus_tag="IW662_001601"
     CDS             3003..3449
                     /gene="msrB"
                     /locus_tag="IW662_001601"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ0866428.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3619..4221
                     /gene="ruvA"
                     /locus_tag="IW662_001602"
     CDS             3619..4221
                     /gene="ruvA"
                     /locus_tag="IW662_001602"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ0866429.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4234..5235
                     /gene="ruvB"
                     /locus_tag="IW662_001603"
     CDS             4234..5235
                     /gene="ruvB"
                     /locus_tag="IW662_001603"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ0866430.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5264..5842
                     /locus_tag="IW662_001604"
     CDS             5264..5842
                     /locus_tag="IW662_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0866431.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5894..6376
                     /locus_tag="IW662_001605"
     CDS             5894..6376
                     /locus_tag="IW662_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ0866432.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6493..6867
                     /locus_tag="IW662_001606"
     CDS             6493..6867
                     /locus_tag="IW662_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866433.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7667..9019)
                     /gene="brnQ"
                     /locus_tag="IW662_001607"
     CDS             complement(7667..9019)
                     /gene="brnQ"
                     /locus_tag="IW662_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0866434.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9166..9756
                     /locus_tag="IW662_001608"
     CDS             9166..9756
                     /locus_tag="IW662_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0866435.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9884..11233)
                     /locus_tag="IW662_001609"
     CDS             complement(9884..11233)
                     /locus_tag="IW662_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0866436.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11401..12141)
                     /locus_tag="IW662_001610"
     CDS             complement(11401..12141)
                     /locus_tag="IW662_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866437.1"
                     /translation="MNEKIIEINHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12154..13746)
                     /locus_tag="IW662_001611"
     CDS             complement(12154..13746)
                     /locus_tag="IW662_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0866438.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14314..14610)
                     /locus_tag="IW662_001612"
     CDS             complement(14314..14610)
                     /locus_tag="IW662_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866439.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14786..15511
                     /locus_tag="IW662_001613"
     CDS             14786..15511
                     /locus_tag="IW662_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ0866440.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15504..16346
                     /locus_tag="IW662_001614"
     CDS             15504..16346
                     /locus_tag="IW662_001614"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ0866441.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16349..16870
                     /locus_tag="IW662_001615"
     CDS             16349..16870
                     /locus_tag="IW662_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ0866442.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17113..17676)
                     /locus_tag="IW662_001616"
     CDS             complement(17113..17676)
                     /locus_tag="IW662_001616"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0866443.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17927..18196
                     /gene="rpsO"
                     /locus_tag="IW662_001617"
     CDS             17927..18196
                     /gene="rpsO"
                     /locus_tag="IW662_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ0866444.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18384..>20508
                     /gene="pnp"
                     /locus_tag="IW662_001618"
     CDS             18384..>20508
                     /gene="pnp"
                     /locus_tag="IW662_001618"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ0866445.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLFKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
BASE COUNT         6696 a         3948 c         4140 g         5724 t
ORIGIN      
        1 actaagattt tacttcagct ttaccgactg cttcgcccgg gcgaacaagt cctgattggt
       61 caagagaaag tcctgcaact ttttcattat tggtcaaagc aacaataatg tcagttttct
      121 tgccagcttg ttctatttct ggcaaatcca tttcagcaat cagggtctct ggtgtgacaa
      181 ggtatccttc tttcacgaat acattgaaag gaacaccctt tagttcaaca gtatctaatc
      241 ccatgtgaac aagtacttcc agcccctcat tagaaagaat accaatggca tgttttgttt
      301 caaagacact cgtcacttta ccgtttactg gtgcatagac tttgccgttt gacggttcga
      361 cagcataacc gtcacccatc attttttgtg agaaaactgg gtcatttact tcagttattt
      421 ccttgatggt ccctgcagcg actgcgaata atgtttcttt tgttgcaact gccgtaccag
      481 ttgctgaaac aggttcgaaa gactgtggcg ttgctgttgc gccatttgct gtttgaagtt
      541 gtacatcagc agcattttca ggaacagcag gatcaatctt attcaaaatt ccctgacgtc
      601 taaagatgat cgtcaagcca aatggaatga tgacagcaat gatcattgcg atgatgaaag
      661 gtacccatgt ttctgcttgg atagctagta ttcctggcaa accgcctact ccgatcgcgt
      721 tagctcgaac acccatcaag ttagcaaaca tgccagctaa tccagagccg accattgcag
      781 cgacgaaagg atagacgtat ttcaaattga ttccgaacat agcaggttca gttacgccta
      841 agtaacaaga aatcattgct ggaatcgaga tttgttcttc ttttttattc ccacgatgta
      901 agaagatgat ggctaagact gcagagcctt gggcgatatt tgataaagca atcatcggcc
      961 ataaattcgt tgatccgaaa tctgcaatca gctgcatatc gatggcattg gtcatatggt
     1021 ggagaccagt aatcactaaa ggagcataca ggaatccgaa tacggcacta aacaaccagc
     1081 tgatagaaga cgtcaatcca gtatttacga tatttgagat ccagcttccg actgtccagc
     1141 cgataggacc taaaataacg tgtgcagcta aaacagttgg taacaatgaa aacaatggaa
     1201 caaaaatcat cgagatcgac tgagggatat atttgcggaa gaagatttct agatacacta
     1261 acataaaccc tgccagcatg gctggaataa cttgcgcttg ataaccaatc atctggactt
     1321 gagcaaaacc aaaatcccaa aatggaatat ctgctgcggc tgtggaagct acactataag
     1381 catttaataa ttgcggagaa actaacgtaa ttcctaaaac gattcctaat atttgtgtag
     1441 tacccatctt ttttgcaata ctccaagtga tacctactgg aagaaaatgg aaaattgctt
     1501 cacccggtaa ccacaaaaag gcattgacac cattccaaaa cttcgagtgt tcaacgattg
     1561 ttccaccaag actttcaaac tggatacctt caagtacatt tctgaatcca aggatcaatc
     1621 caccgacaat gattgcagga atcagtggtg taaagatttc tgctaaaacg gcaatagcac
     1681 gttgaacggg atgaaggttt tgttttgcag ctgcttttcc ttgttctttg gaaacccctt
     1741 caactccaga tactgcaacg aagtcgttat aaaaagtaga aacatcgttt cctatgatga
     1801 cttggaactg tcctgcattc gtgaacatcc cttttacgct aggtatatct tcgatcgctt
     1861 tttcgtccgc ttttcctgga tcattcagga caaaacgcat acgagtcgca caatgactaa
     1921 ctgcagcaat attttctttt ccgccgatat catgtaacag cttctcggca tctgcttgat
     1981 attttgccat atcaataaac ccctttctct taacttgtct atgcaagttg tttcttcatt
     2041 tactatactt tttttcggag agagttgcaa gcgttttatt aaaaagtacg agagaaaatg
     2101 atatcgtttt aaaaagctta aaataagctt ttcttaaaaa taggaacatt gaaatacaaa
     2161 aagagctgta gtatagtgaa gaaatgatgg aagaacgagt gagggaaaaa acatgaataa
     2221 gttcaatgaa atttttttag atttggaaca gaaaatttta gcgaaagaat atcctccgca
     2281 ttcgctttta cccagtgaga accaattgat caaaatttat ggtgtttcac gagaaacaat
     2341 tagaaaagcg ctgaatcttt tgactacgga aggttatatt caaaaaaaac aaggaaaagg
     2401 ttcgatcgta ctggatcgga atcgcttcga ctttcctatt tctggattga ctagctataa
     2461 agagctacag gagacccaac gtattccaag tgaaacgatc atccatacgt tagaagaaac
     2521 agaagtgaca aaagcattga acgagatcac tggctgggag atcggtgcgc ctgtttggca
     2581 tttgatacgc gaaagaaaaa tcaatggcga agtagttata ttagacaccg attatctatt
     2641 aaaggaaatc gttcctattt taactccagc acaagcacaa ggatcgatct atgaatattt
     2701 tgaaaatgag ctttctttga cgattgctta cgcgcaaaag gagatcacag tagaagaagt
     2761 aacggaaatc atgaaaacag cgatggatct tcacgatgac acttatgcag tagtagtcag
     2821 aagcctcgtt catctggaag atacccgctg tttcgagtat tctgagtcgg ttcaccgatt
     2881 agataaattc cgtttcgttg agtttgcccg acgccgaaaa gtgtaaatag ttcaaaagaa
     2941 agcaaattgt tggcatctgt ccactttggc gctatgatag gatcacaaag ggggcatttt
     3001 taatggataa accaataaga ccagaaaaag aagaactgaa agaaaagtta tcacccatgg
     3061 cttatgcagt cacacaagaa aatgcaacag aacgaccatt tagcgggaaa tatgatgatt
     3121 tttacgaaaa ggggatctat gtagatatcg tcagcggtga accgttattc agctctgcag
     3181 aaaaatatga tgcaggttgt ggctggccat cattcagcaa accaataacg aaaagacaag
     3241 tgcgcgaaaa agccgatttt tctcatggca tgcatcgaat agaagttcga agcaaagaag
     3301 cagattctca tctcggacat gtgtttaccg atggaccagt agatcaaggt ggtctacgtt
     3361 actgcatcaa ttctgcctca ctgaaattca ttccatatga aaagatggat gaattaggtt
     3421 atggggaatt caaatcactt gtagaataat ggaaatacac aaagagcggt acaatacggg
     3481 gatttactcg atcgtccgct ctttttttat tcaagcgatt gctatttgaa agaaacgctc
     3541 cccagactcc ttttttcaca aggtgaaatg tgctacaata aagggaacgt atgtgtgcaa
     3601 aagaaagagg actagaatat gtatgaatat ctgacaggtg tggttacctt tatcaacccc
     3661 tattacatag tagtagaaac caatggtatc ggctaccaga tcgcactagg aaatccttac
     3721 cgctacagta gcaaactaaa tcaaaaaata aaaatttatg tccatcaggt aatacgtgaa
     3781 gatgcgcatc ttctgtatgg atttgattcg ctagaagaaa agcaactgtt tttgcgatta
     3841 gtaagtgttt caggaatcgg tccgaaaagt gcccttgcaa tcatggcaag cgaagaccac
     3901 tccggtttga ttcaagcagt agaatccagt gatgtcactt atttgacgaa atttccagga
     3961 gtagggaaaa agacagcaca acaaatgatc ttggatctaa aaggaaaatt cggtgagctg
     4021 agtatcgata caccttttag tctatttgat gaagcaaaca caaaagacgc tacagctcta
     4081 tctgaagcaa tggaagctct ctcggctctt ggttacagtg atcgagaaat caaacgtgta
     4141 gaaaaacaat taaaagaagt agataatcag acaacggatg agtatctacg ccaagcactc
     4201 aagctaatga tgaaaaaata aggaggagac caaatgacag atgaatactt gttatcacca
     4261 gaatctggcg aaaatgaact gagtttggaa aaatctcttc gaccacagct gctttctcaa
     4321 tatatcggac agagaaaagt caaacaagaa cttcagattt acatcgaagc tgccagaaat
     4381 cgggaagaac ctttagatca tgttctgttg tacgggcctc ctggattagg aaaaacgacg
     4441 atggccatgg tcattgctaa tgagatgacc gtaaatattc gtacgacgag tggccctgct
     4501 atcgaacgtc cgggtgactt agtagcaatc ttgaatgaac tggaagcagg ggatgtcctg
     4561 tttatcgatg agatccatcg attacctaga gtggtagaag agatgcttta ttcagctatg
     4621 gaagatttct atgtagatat catggtagga caagggccaa ccgcacatcc ggttcatttc
     4681 cctcttcctc cctttacttt gattggcgcg acgacacggg caggtatgct ttctgcacct
     4741 ttacgagatc gatttggcat catttctcat atggaatatt atgacgaaca ggatctgcag
     4801 gagatcgttt tacgttcttc ggatattttt caaacagaga tcattgaaga aggggcattg
     4861 gaaattgctc gccgatcacg aggaacgcct agaatcgcta atcgcttgct caaacgggtt
     4921 cgtgattttg cgcaagtcca aggagacggg aaaatcgatc gtcctatagc ggatcaggca
     4981 cttactttgc tacaggtcga tcaggctgga ttggattatg ttgaccagaa attgttgcga
     5041 acaatgatcg agttatatgg cggcggacca gtagggttaa gtacgctttc agtaaacata
     5101 ggagaagagc gtgagacggt agaagatatg tatgaaccgt atctgatcca aaaaggattt
     5161 ttaaagcgta cacctagagg gcgaattgct actgcttatg cctatgaaca ttttggttac
     5221 gactatcata tatgattttt gcttttagat tggaggaaaa tgaatgagtg atacaacacg
     5281 tacgaaacaa ttgatcaaaa acgtattgat tgatttgtgc caaacgcaat cttttcgaaa
     5341 aatcagcgtc caaaaaatcg caaatgaagc aaaaatcaat cgacaaacat tttattatca
     5401 ttttacagat aaatacgatt tgcttcgttg ggtctattgt gaggatgcat tgcattattt
     5461 agatccacaa tgtctttcac ttgataattg ggaagagcaa gcattgaaag tgctgaaagc
     5521 aattgctgag aagaaagaat tttatgcaaa tactgtgctt tctgacccag aaattttaca
     5581 aaaagagttt tctcaactga tccaaaaatc atttatcaag atttttgaac aaatggatga
     5641 agaaagacaa ctttctgcaa gagacaaact attttatgct cgtttttttt cttacggttg
     5701 cagtggtgta ttagtcaatt ggatcacgaa tgaatatgaa gaaacaccga tggaaatcgc
     5761 tatgcagctt ttccgtttag ccaaggatac ggaattatat tcttatcgtc tttatgcaaa
     5821 agaagagttg gaagaacagt aatagaaaaa ttttttgttg gaaaagcaat aatcgctaaa
     5881 aggagatggg gaaatgacaa aaattttatt tgtttgtttg ggaaatattt gccgttcacc
     5941 gatggcagaa ggcttgctga gaaaacgaat agcaatagaa ggaagggaag cagaattttt
     6001 tgtggattct gctgctacaa gcacgtatga agttggaaag acaccgcacc ctggaacgaa
     6061 aaaaatatta aaccaagaaa aagtagatat gacgaatatg gttgctcggc aaatcacacc
     6121 ccacgatttt gaaacttttg attggatcat tggcatggat caagaaaatg tagaagaact
     6181 gaaacgaaga gcaccacgct cagcgcaggg gaaaatccat ttgtttttaa gtagcattcc
     6241 tggaaaagaa aaagaaaatg ttcccgaccc atactatacc aataactttg atgagacata
     6301 tcacttgatc acagaaggcc tgaatcattg gtggaatatt tggattgacg aaaataaaat
     6361 aacaaacaat ttgtaaagtt gacgctttct ttttaaggcg atgttagagc atttccttgt
     6421 ttttttgtaa aatgttaggt aaaataaaat taattaagta acactttctt agctaaagga
     6481 ggagtgcatt cgatgaacga agagacagta tcgccaatga agaagcttac gaagttagtt
     6541 actcatactt cgtatcaact ttttgattct tttgtaaatg acactgaaga acatgctaag
     6601 aatgtacgat tgcaaggaaa gtttattctt gagctgcaaa aagctgctac tgaacatagt
     6661 ctagttatct tacaagtaag agcgaatcgt ttgagtagca agtatgaaac gatctcaggc
     6721 tgggtggcga caaaaacggt cgaaaaagac cgagtgatga tccgcctaca aaacgaagag
     6781 cagcagatcc ggatggttcc tgtagaacaa attgaaaaaa taacaatcct ttcaccagca
     6841 ggggaacagg aacgattatc aaaataattt ataccagata gaaaagcacg tatgagcttg
     6901 taccatcaat cattactaat caaaaataac cgaatgcttt aagctgatat cagtaaaacg
     6961 gaacgtttta ggatgagtat cacagcatga cagcattggg ttgattagtt gaatcgattg
     7021 gatggtacaa gtttttttga taaaaaaagg tcctgctagc cggggagcta gcaggataag
     7081 gagttaaaaa tgaaaaagtg ttgttagggt tgtttgttgg tatgcttata taatagtcac
     7141 tagttgtgaa tattttgtga cgaaagtttt cgaaagttgt taagtttttt tgcgaaaact
     7201 atttttatat catatatatg tctaaaatct atattataaa agaaatgttg tttgatcagc
     7261 ttctcttcgg tattagttca aaacaaaacg gcaaagtata tggagctact ttgccgtttt
     7321 gtttctctaa tactcaggtc agtttataga cgctcaataa acggtgttca aaagccagtt
     7381 tctcggtatt aagtcaaatt tatgcaaaaa tgtgagaagc cattttcaca aaatatttct
     7441 taatacttga aactaagcgg ctttttcacg acctctcaat aaacggtgtt caaaagtcag
     7501 tttctcggta ttaagtcaaa tttatgcaaa aatgtgagaa gccattttca aaaaatattt
     7561 cttaatactc gaaactgagc ggctttttca cgacctctca ataaacagtg ttcaagagac
     7621 agcctatcgg caataaggct gaacgtctct ttcacaacct cttttatcaa tctgctactt
     7681 cttctttctt agcgaaactc caaatcagtc cgataacaaa accgataact gctggtacga
     7741 tccaccccat ccccaatttg aaaaacggca agtagctttc cgcaacatat aaaagatttt
     7801 gaacaatagg tgtttgtttg atagtttctg ggcaagcgtt caacccatca aaaatcgaag
     7861 caatcaatgt aaaataagta gtcattcgat aaacagccgt acgctgctta aacaaaggac
     7921 caactaacac tagtaaaatc agtgtcattg ctaatggata gatgaacatc aaaacaggca
     7981 acgaaaattg aatgatattc gtcaagccta cgtttgccgc taaacaagga aggatactag
     8041 caagtgcaac aaagaatgcg taatttcttt gcggaaacaa atctgtgaat gtttctgcaa
     8101 aagctgtaat caaaccaata ccggtcttca agcaagctaa tataacgata aatgctaata
     8161 agatactgcc ataagtacct aaataatgat cagcaatctg agccagcgtg atcccgccat
     8221 tttcacttag agcaaagcta cctaagctca tagcacctaa ataagcgagc aaagtataaa
     8281 tgattcccat caatataatg ctgatcgcac cagatttgat cgtgtcttta gcgatttcag
     8341 caggttttgt tacacccatg ttttggattg ttgtgacaat gatgatccca aatgctaaag
     8401 cagcaagcgc atctaatgtg ttatatcctt gagtaaaccc tgtgaaaaaa gcatgctctt
     8461 gataatttgg ctgaataggc gcttgattta cagaacctag tggattcaaa aaagcaagga
     8521 gaagcaaagc acctaataaa accaaaaatg caggattcag gaactttcca acataatcta
     8581 atatctttgt cggttttctt gaaaaccacc aagccagcac aaaaaataca atactgaaaa
     8641 ttgctagata tagcgtctgt ctgctaggat cgataaatgg tgctaaacca atctcgaaag
     8701 atgtggttgc aaggcgaggt aaagcaaaaa acggtccgat aactaagtaa agcaagatgg
     8761 taaaaaccat cgcatatgtt tgatttatcc gttttgctaa ttcatataca cctgagcttt
     8821 tcgatacacc aatggcaatc acccctaaga agggaagacc gataccggta accaagaaac
     8881 ctagattcgc ccaaaaggta gcagcgcctg cttcttgacc caaatgaacg ggaaagataa
     8941 gatttccagc accaaaaaat agaccgaaaa gcatggagcc tataaaaaga tagtctttaa
     9001 atgataattt ttttgtcata cataactcct ttacggtttt tttaaaagta acaaaatttc
     9061 gaaaaatatg caacaatttt cagaaaattt cctatataaa acgacggtta tttaataacg
     9121 taagtgataa aataaaagcg ctgaatcatc tgaggaggaa tcatcgtgac aaagtcgaga
     9181 ttagaagcat ttactgatgg agtggtagcc attgttttga gagttcttgt attagagatc
     9241 aagatcccta atacatctag ctttcattct ttatgggtca tccgaaatac attattagct
     9301 tataccatca gctttatatt tgttgcggtg atctgggtga accatcatcg tatatttcag
     9361 atggcagaac gaattaacta ccgagtggtc tggtcgaata tctttttgct cttttggctg
     9421 actttatgtc cttctgttac tagttgggtc gggcgtaacc ctgaagcttt ttggcctgag
     9481 atggcttatg tcttagtata cacgatgtgg agtttttctt ttggtatctt gatgaggcaa
     9541 atcatgaaag caaataaacc agattcccat gtcgtgagag ttttgtcaaa ggaccgtcga
     9601 agtcttatct ccatgatgat taacttatgc ttgattgccg gtgtcttttt tgtaccgttg
     9661 atcgggttat tcggacgttt ttttgtatct ggtatttgga ttttttccta taaaaaagca
     9721 gatgcctatt atcagcggtt gtttccgggg aaatagaaaa gaggctggga cagaagcttt
     9781 taactccaag aaataagccg aaatttcaca aaaatttaag aagcaatttt cgtaaattcc
     9841 ttcttatttc tcgaagctga ccacttctgt cacaactact ttcttacttc ttacgttcat
     9901 atttcatatg atagattagt aatagaagca agaaccacac aggtgttagt aataaagcga
     9961 ttctcgtatc ttttcctagg aacaagacga cacacacaaa ggccatgtaa agaaatacca
    10021 gataattact aaatgggtac aacggcattt taaatgaatg agcttttttg ccgaggatac
    10081 ttttacgata tttcatgtgc gtgtagacaa gcatactcca gataaacaag aaacaagtag
    10141 tggcaatact gcttactagt gtgaatactt cactaggcat cacgtaattc aagataacag
    10201 aaatgaaaat gacgatagta gaaaatgtca aagcattccc tggcacatga tgtgttgtta
    10261 actttttgaa ttttttaggg gcactgcctt cttgcgctaa agaacgaagc atacgtcctg
    10321 tactatatat tgcactgttg caggcagaag ctgctgcact taatacgact aaattgatca
    10381 aggaagcagc aatcgtgatc ccaatttccg aaaatacttc tacaaatgga ctgttatctg
    10441 gattcaagct gttccatgga tagattgcca taatgacaaa aagagatcct aagtaaaata
    10501 agatgatcct gataggaata ttattgatag cttccggcaa cactttttca ggattttctg
    10561 tttctccagc gactaatcca actaattcta tcccagcaaa agcaaaggtt accatttgga
    10621 aagctaagat aaatcctttt gcaccagttg gaaagaatcc gccatgagac catagattcg
    10681 tgatactggc tgtgccaata tctgtcgtat agtgggtgag gatcatataa gctcctacga
    10741 ggataaaggc aatgatcgct acaactttga tcaatgcaaa ccaaaactct aactcaccaa
    10801 acaatgagac agccaccata ttcaagcaaa gaagaagacc taatgcaatc agttcaggaa
    10861 gccactgagg aacaccaggc gcccagtagc gtacatacat tccgatggct gttaaatcgg
    10921 ccattgcaat cgatacccag cagaaccaat acgtccagcc agtgatgaaa gccgcttttt
    10981 tgccaagata ttctgcaaca aaatccagaa aagaatggtt attggtgttt gataacagaa
    11041 gttcgcctaa cgcacgcata atgaaaaaac agatgcatcc agtaataaga taggcgagca
    11101 atatcgaagg gccagctagc tggatagact ttccggaacc taaaaacaac cccgttccaa
    11161 tcgctccgcc aatagaaatc agttggacat gtctattttt cagtccacgc tgcagttctt
    11221 gctcttcatt catttttaaa tcatcctaac taaaaaagtt taattacttc catattaaaa
    11281 gagttttctg aaaaacacaa ctaagattcc aaaattttca gaaatttacc ttttatgagt
    11341 aattcataaa aaagaactgc ggtaaacacc gcagctcttt gtcccaatca gctattgatc
    11401 ctaagcattt aaaatacgtg aaaggaattc ttttgtacgt tcttctttcg gatggacaaa
    11461 gatatcttct ggtgtacctt cttctgcaat cacaccttta tccataaaaa tgacacggtc
    11521 agatacatct tttgcaaatt ccatctcatg cgtcacgata agcattgtca agccagtatg
    11581 tgctaagtct ttgatcgtat taagcacttc gccaaccatt tcagggtcta acgccgaagt
    11641 aggttcgtcg aatagcaata catctggatt cattgataat gcccgagcaa ttgcgacacg
    11701 ctgtttctgt ccaccagata attgtgctgg tttggcttcg atataacgat ccataccaac
    11761 tttttccaga ttttcgattg ctactttttt ggcagtttct ttatcacgtt tcaggacagt
    11821 tgtttgacca gacatacagt tttccaagac attcatattg ttgaacaggt tgaaggattg
    11881 gaaaaccata cctagattcg ttcgatattt aggtaaatta tatttaggag ctaatacatt
    11941 ttctccatta taaatgatct cgccgcctgt tggtttttcc agtaaattga tacagcgcaa
    12001 gaaagtagat ttaccagaac cggaagaacc gatgatcgtc acaacttcac ctttattgac
    12061 tgttacgctg atatctttca atacttcatt ttcaccgaag ctttttctta gatgattgat
    12121 ttcaataatt ttttcattca tgattcttcc tccttattcc tgcaaatttg aattgtcgat
    12181 ttcttcaagc ttggtataag ctgaaggacc atcgattttc ttttctaccg cccgcaggat
    12241 tctggtgatc gtaaaggtca tgactaagta gatgatcccg acgatcgtaa atgtttggaa
    12301 gaattggaaa ttcgcaccgg aagcagagtt tccttggaag aacaaatcag ctacgccgat
    12361 tacacttaaa actgctgtat ctttgatatt gataacaaac tcatttcctg ttgctggcaa
    12421 gatattccgt aaaacctgtg gaataacaac ttttctcatc gtttggccat gggtcatacc
    12481 aatggcttgc gcagcttcga actgcccttc atctactgca aaaatccccc cacgaacgat
    12541 ctctgtcatg taagctccag tattgagaga gacaataaac aaagcggcaa tcgttcggtt
    12601 caatgaaatg ccaaaagcga gtgccagacc gtagaagatg accattgctt gtaccatcat
    12661 cggtgttccg cggaatactt cgacatagat agaaagaagt gcatttccga ttttttggaa
    12721 gaagcgagtc agtgcatttc ctgattcagg taccgaacgg aagacaccaa tcagtaatcc
    12781 aagtgtagta ccgacaactg tcccgatcaa tgcgatgaac aaggtaagtc cagcacctcg
    12841 taagaacata ttgccgtatt gtttccaaat cgtagtgaaa tcagaaatga tgccaggttt
    12901 ttcttcacta tcagttgcag caggctgatc tttgactgct tggtccataa ttttggttcg
    12961 ttcatcgtta gagatgccag aaagaatctg gtttactttt tggatatccg ggtcgccttt
    13021 acgcatacca acagaaatct gaacatcttc cgggtttgtc tggaaaccat ctgcaccgct
    13081 gaattcaagc atttccaagt ctttgttcac actagttgcc gttacacctt ccggacgttc
    13141 gctgacataa ccatcgatca ttccagaagc taaagctgtt ctcattgctg agaaattatc
    13201 cattgcttgt tgtttctgca catctggtat ctgatcgatc acagtatagt ggaatgtatt
    13261 taactgagca gtgatctttg ctccagaaag atctgctaaa ctttttgctt ttgcatattt
    13321 gctatctttt tgaactacta ctactaattg tgattcgtaa taaggatcgg taaaatcaat
    13381 ttcttttcgt cgttcagcag tagggctcat tccggcgatg atcgcgtcga tcttgcctga
    13441 ttgaagggca ggagccagac catcccattt cgtttggacg atgactaatt ttttccctaa
    13501 accatctgct acttttttag cgatttgaac atcataaccg ccggcgtaac tattttggcc
    13561 ttgaattggc aaggcattat tctcatctgt ttgttgagac cagttgaagg gtgcataccc
    13621 agcctccata cctacgcgaa actcatcgct cggtgttttt tcatctgcat ttgccagaga
    13681 tcccacgctg aatagtgaga agacaaaagc aaatagtacg gcaactgaaa ccatattttt
    13741 tttcattttt ttctcccttt ctgattatat gatacacgag cggtctagat gaaatgcggt
    13801 aaagctttat ctgaaaaaat aaaaagcgac cgatttatag atataaaaag gccgctaaaa
    13861 ttccataagg tgataaacct ctcacaaaac atagcgcaac ttagcatcac tgctaagaca
    13921 gtccgcaagc tcttaacttg cgtcccaaca tacactccaa gcaaaaaagt gcacatttcg
    13981 gcgatcctcc ctattcctgc ttctccatgt ttgcttcact ccacagggtt aatgattctc
    14041 gcgacctcta cctcattgat tgaggcatct cgtattcagt tatttataca aaaaaagttt
    14101 acgatactcg agaggctttg tcaatctata actgctgttt tttgaaaaaa tactgataaa
    14161 tatagaaatc tttactttta ccgaaaaaac aatcaaaatg tgaatcatct atccattgta
    14221 aacgatagta taattataca caaaaatgta tgtaatgcaa taaaaaaaga actagaacag
    14281 aaaatttcct gtgtccagtt ccatctcgat catttaagct gccgctttct ttggctcacg
    14341 tcctaggatc gcttgcaaaa gaacaacgac gccataaatg gctaatatta gataaatcaa
    14401 acttgagtat tgggtgattc cccagcccca tgttcgatat acggctaatg taatggctaa
    14461 aacaattgcg gcaatcacgt tgaataaagc aaaagcccaa agatttccat tttctttgcg
    14521 tacaagataa aaaacagaga aataaacgct taacgctaac caggcgatac aagccagaat
    14581 aacgccccaa taaataaaga atggtaacat atcgattcac ctcactttta tacagcagta
    14641 ttgtaacacg ttttcattct tttgtcagtg aacattgtga tgaattgctc aaatgcccat
    14701 aacacttttt tattcccatt tttctgactt ttcaacaata aatttgttcg agtatgggct
    14761 ataatggaaa gtgaggtgac aaaacatgaa acaattaaca gtagacgaag cagcaaaaga
    14821 gatattagct tatcagcaaa ttacattttt gaccggtgca ggggtatcca caccttctgg
    14881 agtgccagac tatcgatctt tgtcaggaat ctaccacggg atggagcagc cggaatactt
    14941 gctgagtcat caagcaatgg tgaatgaacc ggataaattc tatcaatttg tcaaaaagat
    15001 ttatcatcct gaagcaaaac ccaatatcat tcatttggaa atagcacatc tagcgaaaga
    15061 aaaaaatgtt tgggttgttt cccaaaacat cgacggtttg cacgcaaagg ctaaaagccc
    15121 gcaactcgtc aattttcatg ggagtctgta tcaatgtcat tgtcgaaagt gtcaacaaac
    15181 ggtagactgg aaagaatatc ttcacagcga caaacatcaa gcttgcggag gacaaatacg
    15241 cccagacatc gtgttatatg gtgaagggtt tcaagatgga gtcatggagc aggcggctta
    15301 tgccgtcagc caagcagagc tgatcgtcat tgttggaacg agttttcaag ttcatccgtt
    15361 ttgtgatctg gtacagtttc gccagccgtc agccaaaatc ttagtaatca atcaaacgcc
    15421 tgtttattta gcggagcctt atacgtttat tcaagaagac ggagcggttg ttttcaaaaa
    15481 aatacaagaa ttaggagcag attatgactg aattcaaaac cgaaaaagaa aaaatgattg
    15541 caggcgagct ttattttgcc aatgatcctg agctggttgc cgaccgtatg tttgcaagaa
    15601 gtcaaagcca aatcatcaac caagcagaat cagcggaact gcgatcccaa ttgttgaaag
    15661 agacttttgg ccgtacaggg aaaaaaatct atatggagcc agttatcaat tttgattacg
    15721 gatataatat atttgttggg gagaattttt atgcaaattt caattgtaca tttttagatg
    15781 tcagcacgat tgaaatcggt gataactgca tgttcgctcc gaatgtccag ctttacacgg
    15841 cgacgcatcc actacatcca gtgaaacgta acagcggtct ggagtacgca aaacccatta
    15901 aaatcggtaa taatgtttgg ttaggcggag gagttatcgt cacgccgggt gtcactctag
    15961 gggacaacgt agtagtcggc gcaggttcgg ttgtgaccaa atcttttccc gacaatgtag
    16021 tgattgcagg aaatcctgct cgtatcatca agacagtaga ggaagaacta acagaagagt
    16081 ctcttgaaac attacgtcat aagatcgata tgatcgatcg agagttggtt gctttattag
    16141 aaaaaagaat ggatacggta actaaaatag gacaagtgaa aaaagaagaa aaccaggcag
    16201 tttatgatgc aaaaagagag cagcaagtat tggataaagt tgttagtctt ctgaaaaata
    16261 agggatataa ggaaacaata acagacacgt atgttgattt gatgaaacac tcgcgcgaat
    16321 accaaaacaa aatgaaggag gaataagtgt gatcgaagga gagacacggc gaaaacaaat
    16381 catgcaaacc ttggcaaata cagaaaaacc agtgagtgct agcaagtttg cgaaaacttt
    16441 tggagtcagc cgtcagatca ttgtaggcga tattgcttta ctacgagctg caggtaaaag
    16501 tatagtggca acagcaagag gttatatcct cgaaacagaa gaaaccaata ctgggcacat
    16561 cagtaaaatc gctgtccagc atggaaaaga tcaaacagaa gaagaactga gactaatcgt
    16621 tgaaaatggt ggagaaatca ttgatgtaat tgtggaacat cccctttacg gtgagttagc
    16681 aggaactttg cacatcaaaa cgccacaaga tatcgattcc tttatgagac gttatcaaaa
    16741 aagtaatgct actttgttat ccgaattaac aggcggcatc catctgcata cgatccgcta
    16801 cccagaaaag catatgctga aaaacatcaa acaaaaacta gctgaagcag gaatcctcta
    16861 cgaaggataa catcgtagct gctacttttt atcctagaaa ctattctatc atctctgaga
    16921 taaacaccca tatggaaaaa ataagaaaag atgcgtatcc aatacacaga attttttgga
    16981 attgcgattt ctagatgtct atctaaattt cgattagtgg agaaacataa aaaagggaag
    17041 tgcagctttt gagcactccc cttttttata aagctttctt gtagcatagt atcgaaggaa
    17101 cctgccaatt ctctactcga ttaccaatac gccttctttt agtgcaaatg gattttgatc
    17161 gttgatgtgg tcatagaaca tcacaccgtt gatatggtcg atttcatgtt gaacgacgat
    17221 cgattcataa ttcttcaaac gaatcttgtg tttttcacca ttcatatcat aataagatac
    17281 agtgattttg gcatggcgaa caacatatcc tggtacttct cgatcaacag ataaacagcc
    17341 ttcaccttcc cctaaacaag catcttgaac agaatggctc aaaattttag gattatacat
    17401 aacagtactt agagaaggtc catctgcctc tgggtcagga cttggtacgt gaacagcaat
    17461 gattctttta gaaatatcta attgaggagc agctaagcca acaccgcctc gtaagtgtag
    17521 ttcttcagct ttgattggat cttggctgtt ttttaagaac tcaagcatct cttttcctaa
    17581 agagatatct tcttcgctta atggaagaga aacttctttt gcaacttcgc gtaaggtcgg
    17641 atttccttca cgaatgatat catccattgt aatcatggaa tatgtacccc tttcttaaaa
    17701 gactgtgttt caagtctatt ttcgcattat agtttaccac aaaaatggaa aagtagtagt
    17761 aagattgttt gaaaactacc ttttcttaat gattaactac ttgattttca acccggtttc
    17821 aggtaaagta tagctgtgtg ctctaggcac atgacctgcc acttggttaa gcgaatcacc
    17881 aacgttttac tcagtcgcag gggaataatc gaaagaggtg aaagaaatgg caatttcaaa
    17941 agaacgcaag aatgaaatca tcaacgaata cgctcgccat gaaggggata ctggttctcc
    18001 agaagtacaa atcgctgtat taactgaaga aatcaaccac ttgaatgaac atgcacgcgt
    18061 gcataaaaaa gaccatcatt cataccgcgg cctaatgaaa aaagttggtc accgccgtaa
    18121 cttgttagct tacttacgta aaactgacgt tcaacgttac cgcgaattga tcaaacgttt
    18181 aggcttgcgt cgttaattgt agtcaaaaag agtgggattc acatgaatct cacttttttt
    18241 actcgtatat caattaagta aaattccgtg atcaagctag atggactcct actaaccaaa
    18301 tgaaagctgc tcaaaagacg gctttcggca gttttcattt gtttagtagc gtcttgcttt
    18361 cacgagaagg aaaaggagaa gttatgtcag aaaaacaagt attcaaaacg acatggggcg
    18421 gccgtccctt acaaatcgaa gtcggtcagc tagcaaaaca agcaaacgga gccgttttag
    18481 tccgttatgg agatacagta gtcttaagtg cagctgtggc ctctaaagaa gcaaaagata
    18541 cagacttttt cccattaacg atcaattatg aagaaaaaat gtatgcagca gggaaaatcc
    18601 ctggaggttt catcaaacga gaaggccgtc caagtacgga agcaacattg actgcccgtt
    18661 tgatcgaccg tccaattcgc ccgatgtttg cagaaggctt ccgtaatgaa gtccaagtaa
    18721 cgaacatcgt gatgagtgta gaaacagact gctcgccagc tatggcagca atgctaggtt
    18781 cttctttagc actatcaatc tcagatatcc catttgacgg cccaattgca ggcgtagaag
    18841 ttggtcgtgt aaatggcgag tacgtcttga atccaacagt agaacaagca gaacaaacag
    18901 atatcgaatt gactgttgcc ggaacgaaac aagccatcaa tatggttgaa agcggcgcga
    18961 aagaagtttc agaagaagac atgcttggtg cattgctgtt tggttttgac gctatcaaag
    19021 agttagttgc tttccaagaa gaaatcgttc aagcagtagg gaaagaaaaa atggaagtga
    19081 ctttactgca agttgatgaa gtattgaaaa aagaaatctt tgatgcttct tacgctacaa
    19141 tgaaagcagc agtaatgact gaagaaaaac ttgctcgcga agataatatc gagcaagtga
    19201 agatcgatat ccgcgaagct tatgctgaga aatttgcagg tcatgaagat gaagatcact
    19261 tgttgaaaga agttaaacaa atcacagaag atcttgaaaa agatgtcgta cgtgaattga
    19321 tcaccatcga taagatccgt cctgacgggc gtaaattgga tgaaatccgt ccattgtcat
    19381 cagaggtcag cttattgcca cgtgtacatg gatcaggttt gttcacacga ggacaaacac
    19441 aagctttatc agcttgtaca ctcgcacctc taggagagca ccaaatcatt gatggtttag
    19501 gggtggaagt cagcaaacga ttcatccacc actacaattt ccctcaattt tctgttggtt
    19561 ctacaggacg tgcaggctct ccagggcgtc gggaaatcgg acatggcgca ttaggtgaac
    19621 gtgcgttagc acaagtaatc ccttcagaag aagaatttcc ttatacgatc cgtttagtag
    19681 cagaagtatt ggaatcaaat ggttcttctt cccaagcaag tatctgtgct ggcacattag
    19741 cattgatgga tgcaggtgta ccaatcaaag caccagtcgc cggtattgcg atgggacttg
    19801 tttctgatgg tgaaaactat acgatcctaa ctgatatcca agggctggaa gatcatcttg
    19861 gcgatatgga ctttaaagtt gccggtacaa aagatggtat cactgcttta cagatggata
    19921 tcaagattca aggaatcaca gaacaaatcc taactgaagc attaactcaa gcaaaacaag
    19981 cacgtatgga aatcttagaa gaattgactt caacgattgc tgctccacgt gaagaattaa
    20041 gccaatatgc accaaaaatc gaaatgatcc agatcgaacc tgctaaaatc aaagatgtta
    20101 tcggtaaagg cggcgatacg atcaacggaa tcatcgatga aactggtgtg aaaatcgata
    20161 tcgatcaaga tggaaaagta agtattgctt cagctgatgc agaaatgatc aaaaaagcta
    20221 tcaagatcat cgaagatttg accaaagaag tcaaagttgg tgaagtttat ctagggaaag
    20281 ttgtccgtat cgaaaaattc ggtgcattcg tcaacttgtt caaaggaaaa gatggacttg
    20341 tccatatttc ccaattagca aacgatcgtg tcaataaagt cgaagatgtt gttaaactag
    20401 gtgatgagat ccttgtgaaa gtaactgaaa tcgataaaca aggacgcgta aatctgtcaa
    20461 gaaaagcaat gttgaacgaa gatggttcaa cgaaaaatga agaaaaat
//
LOCUS       DACEKH010000049        19827 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEKH010000049 DACEKH010000000
VERSION     DACEKH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.050"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(128..343)
                     /locus_tag="IW662_001619"
     CDS             complement(128..343)
                     /locus_tag="IW662_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0866446.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(362..598)
                     /locus_tag="IW662_001620"
     CDS             complement(362..598)
                     /locus_tag="IW662_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0866447.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            856..3009
                     /locus_tag="IW662_001621"
     CDS             856..3009
                     /locus_tag="IW662_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAQ0866448.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2978..4393
                     /locus_tag="IW662_001622"
     CDS             2978..4393
                     /locus_tag="IW662_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAQ0866449.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            4426..5460
                     /gene="sppA"
                     /locus_tag="IW662_001623"
     CDS             4426..5460
                     /gene="sppA"
                     /locus_tag="IW662_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0866450.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            5462..5983
                     /locus_tag="IW662_001624"
     CDS             5462..5983
                     /locus_tag="IW662_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0866451.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5980..6366
                     /locus_tag="IW662_001625"
     CDS             5980..6366
                     /locus_tag="IW662_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0866452.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(6862..6934)
                     /locus_tag="IW662_001626"
     tRNA            complement(6862..6934)
                     /locus_tag="IW662_001626"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6899..6901),aa:Lys,seq:ctt)
     gene            7315..7884
                     /locus_tag="IW662_001627"
     CDS             7315..7884
                     /locus_tag="IW662_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ0866453.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            7871..8731
                     /locus_tag="IW662_001628"
     CDS             7871..8731
                     /locus_tag="IW662_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0866454.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            8921..9625
                     /locus_tag="IW662_001629"
     CDS             8921..9625
                     /locus_tag="IW662_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0866455.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            9630..11465
                     /gene="walK"
                     /locus_tag="IW662_001630"
     CDS             9630..11465
                     /gene="walK"
                     /locus_tag="IW662_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ0866456.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            11462..12778
                     /locus_tag="IW662_001631"
     CDS             11462..12778
                     /locus_tag="IW662_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866457.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            12779..13648
                     /locus_tag="IW662_001632"
     CDS             12779..13648
                     /locus_tag="IW662_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866458.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESINAFSNT
                     IVTNNTVPKVEDQ"
     gene            13703..14512
                     /locus_tag="IW662_001633"
     CDS             13703..14512
                     /locus_tag="IW662_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0866459.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(14615..15244)
                     /locus_tag="IW662_001634"
     CDS             complement(14615..15244)
                     /locus_tag="IW662_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAQ0866460.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            15414..16706
                     /locus_tag="IW662_001635"
     CDS             15414..16706
                     /locus_tag="IW662_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0866461.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(16782..17444)
                     /locus_tag="IW662_001636"
     CDS             complement(16782..17444)
                     /locus_tag="IW662_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0866462.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            17669..17953
                     /locus_tag="IW662_001637"
     CDS             17669..17953
                     /locus_tag="IW662_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ0866463.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            18004..19629
                     /gene="groL"
                     /locus_tag="IW662_001638"
     CDS             18004..19629
                     /gene="groL"
                     /locus_tag="IW662_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ0866464.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         6724 a         3252 c         4049 g         5802 t
ORIGIN      
        1 tataaaaact agcacaagaa gctttagtaa aaagttctgt ccattccaaa cttttgaaaa
       61 gataaagaac atacctatgt tcgtaatcga cacaaaccaa tcttgaggtt tgatattttt
      121 taattgttta cgcaccggtc cacatgctga atccagcacc taataaagct ccacctacac
      181 cacaagcagc tattgcataa ggtcctccgg ctgaacaaag ttttactcct gctgttgccc
      241 cagaaaaagt agtgcctaaa actcccacgg cactcccacc ttgaatattt ttcaactctt
      301 ctgaagataa tataaatgtt gtttttgtgt ttttaatatg catactactt attctccttt
      361 tttatttttt tcgagcatcc cagccagctg cagcacctcc agcgacagct ccatataacc
      421 ctccaacaac accgcaagct ggtccgcaaa ttgctgttcc taaacctgca ccacctactg
      481 cccctgtggt aagtccacct acaacttgcc ccaattttcc ccaagcatta ttgcctccac
      541 caacagtttg tgcatttcca caatacttag ttcctttaca ttttgcatgt ttaaacacgc
      601 ttctttaaat aatatttttt acggtgctac tgtaacatac gtatttgatt aacagtaaaa
      661 atcttcctga aacttatcaa gtttattctt gaatagtaaa atttccaaaa taaatttgat
      721 tcaggaatga aaaagttagt gttcaagaac tgttttcaaa gtttttgtgg aattatgaga
      781 caatattttc actgtattaa agctatattg agaatttttc tataaaaatt ttttagaagg
      841 agttgtgtta gataagtgat aaaatcattt ccttttgtgc agcaacaaga tgaaaaagat
      901 tgtggtgttg cttgcctttc gatgatttta aaatatttta aaactgatat cccgattcat
      961 aaattacggg acctatctgg gactgattta gagggtactt cagcatttgg attgaaaaag
     1021 acatttgaaa aattgtcttt tgattgtatg gctattcaag cagatagtga gatctggaaa
     1081 gaaagagatc ttatcttacc attaattgca catatattgg ttgataacag ctatatgcac
     1141 tacgttgtaa tttataaaac gaagggaaac gtactatgga ttgcagatcc cgctaatgga
     1201 aaaaatagaa aaacgattga agcgtttcaa aaagaatgga caggtattct tttattacct
     1261 atgcctagag aaacttatat tcctattaaa gaaaaagtag ctggattaag ttcttttttg
     1321 cctataattt ggaaacaaaa aggattagta ttccacatcg ttttagcatc gattttcatt
     1381 acgttttttg gcataggaag ctcatattat tttcaaggta ttcttgattt tttcatccct
     1441 aatcaagcac gttcaacttt aaatataatt tccattggct tgatcgctgt ctatttattt
     1501 cgggtattgt ttgaatatag tcgtagttat cttcttatta ttttaggaca gcgaatgagt
     1561 atggcaatta tgcttcagta tttcaaacat gtgttaactt taccaatgaa tttttttgct
     1621 acgagaaaat caggggaaat tatttctcgt tttcttgatg ccagtaagat tattgatgct
     1681 ttagcaagtg ctacgttatc aatattttta gatattggta tggtgttgct tgttggggtt
     1741 acgttagcta ttcagaatag tacgttgttt ttaatcactc tatgctcttt gcctttttat
     1801 gtagttgcaa tattcgcttt cgtgaaaagc tatgaaaaat cgaacaggga agaaatggca
     1861 gccggtgcag ctgtaaattc tagtatcata gaaagtctta aaggaataga aacaattaaa
     1921 gcatatagcg gtgaagagaa agtatataac cgtatagacc atgaatttgt taaattaatg
     1981 aaaaaatcat ttcgtacggt tacgcttgat aatattcaac aaggaattaa acatttgatt
     2041 caattaatta gcagtgcatt aattttatgg ctaggttctt attttgttat ggataataga
     2101 attagtctag gtcaacttat cacatacaat gctttactag tatttttgac agatccttta
     2161 caaaacatta ttaatttaca agtgaaaatg caaacggctc aagttgcaaa taatcgttta
     2221 aatgaaattt tcgcgattga ttcagaaaaa tcgaaccagt cttttgaata tatgctttct
     2281 gaacaattat ttcaacaaga tatcactgta aaaaacgttt cattctcgta tagcatgaag
     2341 gccccaacgt tgaacgaaat ttcatgtaca attccttcgc acagtaaaac ggctatagtt
     2401 ggagttagtg gttctggaaa atcaactctt gcaaagttat ttgttcgttt ttatgaagca
     2461 tcagaaggat cgatccatta tggaaacatt aatcacttag acattccttt tcagatatta
     2521 cgcgatcgag tgacatattt gcctcaagag tcatttttct ttagtggaac aatcattgag
     2581 aatcttatat tcggtttaga tagcccacca agtttcgaac aaataataga agtttgtgag
     2641 atagtccaac taaatgaatt tattaatcaa cagccattac gttacgagac attgttggaa
     2701 gaaggtggaa ataatctttc aggagggcaa cgacaacgat tagccttagc acgggcttta
     2761 ttaaaaaatc cggacatatt aattttagat gaagcaacta gtggattaga taccttgtta
     2821 gaacatgcaa ttatgaatta tctcctcaat ttaaaagaaa aaacgattat ttttattgcg
     2881 catcatttac caatagcaaa agcatgtgat caaattttag ttctccatga agggcatttg
     2941 gtagaaaaag gaacgcacga tgaacttcgt tttgccgatg gtatctatca aaaattatgg
     3001 gaaatctagg agagggaaac aatttatgca gaataataat tggttcgaac attctagtat
     3061 ctatactaaa caacatcata gcttctaccg ttgggtgtta taccctgtaa ttcttttttt
     3121 atgtattatt ggattgtttt taacctttgc taaaaaagag gttgttattc ggacaacggc
     3181 tcaattgaac cccgaaaaaa tagagaagtt acaagtgcca ttagaagcta aaataatgga
     3241 aaacttccta aaagaaaatc aatttgttca taaggacgat gtgttggtta gattggattg
     3301 ttctttaata gagaatgaaa aggctcagat agaacaagaa aaccaacgta ttactcaaca
     3361 aataaagatg gctcagctat ttattgaaag tataagtaaa ggaaaaaatt tgttttcaac
     3421 ggatgacagt tttggctaca gtaatcaatt aaagagcatg ttgtcagaaa aagaatcact
     3481 ccgctacgct ttgaagcaaa gtgaattaaa tgatcaaaag caattagaag tatacgaaaa
     3541 gacaaaaaga caactagaaa aacaaattga gagttcagat agtaaattac aagaatggca
     3601 acaagtacag gtagcttgga gtaataatca atcattaaaa gatttttcaa aagaaatgat
     3661 ggcaaactat gagaattggc aagaacaact aaataatgtt tctgatgatc aaaaaaatca
     3721 agtgaaactg acaatttcag caagcataaa tgaacaaatt gagcaactaa aaaaagaagt
     3781 agaacagtat cagtcagaaa aagctaaatt agttaaacca actacttctg agaatgacag
     3841 aattagtcaa acggaaaaag gaaagcaaga gctagaacaa gcaataacaa ctgtaaaaca
     3901 aacagttaca gagctaaatg aaaaacaaga gaagaaccag tcagttatca aaagtttgga
     3961 cgatcaactt tcaaaaggaa tactaaaagc tcctgtatct ggaacggttc atttaaatga
     4021 agaaacaaag ggacaagcag aactagccaa aggaacagtg cttgcagaaa tttatccgat
     4081 ccacgaaaaa tcaaaaatga aatttacagc acttttacct gcgaatgaaa gtacctatat
     4141 taaagaaggg atgaaggttc attttaaatt ggatcaaaaa ggtggggctc cgattacgat
     4201 cgacggaact ttagatgaaa tttcagaaaa ttctactgcc actgagaaag gtgtttttta
     4261 tgcagtgaaa ggaacgttaa aaacaactca atcgtttccg tttcgttatg gattaactgg
     4321 agagttgtct ttaattgtag gagaaaaaac atattggcaa caaattgtcg atgtgtttct
     4381 aaataaaaaa taattatttt tactaagaat ataggaggag tttatatgaa taaaagaaga
     4441 tggattgctg tgggagtagc gtgcggatta ttgttactat ccattatcgt cgcgctaatt
     4501 ccaggaaaag ataaagagga agcatcaaat acaaccctaa caggaataaa taagattttt
     4561 tacggatcta acgaaatcac agaagaaacg ttagaagaag gcgcatcaaa taaaaagatt
     4621 gtcaaactat ccgtcaatgg agttattgca gatacaggtg aaagcaatct ttttagtagg
     4681 gaacaatata accatcagaa ttttttaaca cagctaaaga aaattcaaga agacaaagcg
     4741 gttaagggag tgttattaga agtaaattca cctgggggag gaatatatga aagtgctgag
     4801 attgctaaag aaatggcaaa aattaaaaag ctagatattc ctatctatac tgcatttaaa
     4861 aatacagcgg caagtggagg gtactacatt tctgctggtt cagataaaat atttgctacg
     4921 gaagaaacaa caactggatc aattggtgtc attatttctg gtttaaatta ttctggctta
     4981 ttagaaaaac ttggcgtaac tgatgcgacg tataaaagtg gtgcattaaa agatatgatg
     5041 tcacctcaac acaaaccttc agaggaagag aataaagtta tacaggaatt tgtgatgagt
     5101 gcttatgatc gttttgtcaa tgttgtagca aaaggaagaa atatggatac taatgcagtg
     5161 aaagaacttg cggatggaag aatttatgat ggtaaccaag cagtagaaaa tgggttggtg
     5221 gatcaaattg gttactctga agatgcatta gattccctaa aaaaagagaa gaaattaact
     5281 gatgcgacgg ttatagaata taaaaatgac acaactggct ttgcttcttc ttggttaggt
     5341 aataagattg ctgaatggca agggctaaag gcaactacat cagatcgtgt aatttcagtt
     5401 tttgaaaaac ttggattttc tgatactcca aaaccaatgt attactatgg agggctataa
     5461 aatgaataag aagagtggca ttgaaacttt gcctaattat gtatttgcgg gattttggtt
     5521 acgatttttt gcttttctta ttgatttaat ttgtattagc atcatcaccc atgctactat
     5581 aggtctcttt tacaaaatca tgggactgga atctgacaaa tccattatta ctttttatgg
     5641 tatagcgtca ttagctattt atttaaccta ttttgtactt ttgactaagc tgaattcagg
     5701 acaaacaatt gggaaaatga tttttgggat aagagtagtg agctttgagg aaaaagagct
     5761 aagttggcaa actatcttta ttcgtgagtg tatatgtagg tttatattaa aaaattttgt
     5821 atttcaaatt gggtatttgg tggctgcttt cacgcccaag aaacaacata ttggggatta
     5881 tttttctaac acgtcagtgg ttgttattcg ttcattagaa gctattaact atggtcaaaa
     5941 aatttctaat tctaaaacac ttaatctgga gagaattaaa tgaataagag aaaaacagag
     6001 tttattttga ttttaattag tggaataaca gggattttat gtagtctttt agcattgatg
     6061 ggtggtatgt ttctatcttc tattgattca actactttaa atactacaga actaaccagt
     6121 aaggatatag atatgttagt caatctaagc gggaatgttg ttttatttgg aggaattacc
     6181 ctagtttttt cgataatgat tttcataata ggttttttag taagaaaaaa tcaaaaaaca
     6241 gtggtctatg gtattagttt actcatttta tctattattc cattttttct tttaacatta
     6301 ttgtgggtaa ttcctggagt tttaggtatt attgcgggat tgatgcttct aataagaaaa
     6361 tcttaattag gcaatgtgta gacaacgtga aacgattaat caattaaaag ctaaatacgt
     6421 tttgagaaat gataacttgt agtcgatgga agacatgtag ataaaaaaac tacgtgtctt
     6481 ttatttattc taattttttc gaaaaaaaga cactctctga ttgttgtatt aatggaaaag
     6541 gggaaagcag caagtaaatt tagtttttct attaaatatt cttcactttt taacctttta
     6601 tagtacttag atttaatcct atatgtttag agagttcatt ggcagcatga ttttgcaaag
     6661 cttctcgaat tattgttata ttagaaactt tactgttcat aattatgctt cttttttaac
     6721 tcaaagtcca aagggctaca gtggactttg agttaaaaat aaaaagccct agttattttg
     6781 aaaaataaaa aaagaacagc gtaaatgttt tcgaaatgct ttcgcaccaa catttacctg
     6841 ttccgtgggt ttggcaccca atgggccgtg aggggctcga acccgcgacc cgctgattaa
     6901 gagtcagctg ctctaccaac tgagctaacg gcccgtacaa aaagtactta ataatagtag
     6961 cactaagaat tttaaaatgc aagactacat ttaaagaaaa tcagaaaaat ctttacttcc
     7021 ttataggaaa tcattcatag attattcaca tttgctcgct attatataag cataccaaca
     7081 aacaaagttc ttgcaagaga acaaacccta acactttttt cattttttac tcctttcccg
     7141 ccgtctcccc ggcgggtctc ctgttcttaa cgataaaaga caagaaggct tagccttctt
     7201 gttttttgtt ttcatggaat cccgctaatc aaatcaccta gttaaagcaa ctagattgaa
     7261 tgtttatcat aagtctgata tcatgtttat gaaatgaaaa aaggagcggt tatgatggaa
     7321 aaaatgcgtc agcaattaat ggagtggggg aaggaactag aaacattccg tctgcctcac
     7381 tgggaagaac tccctgactt agaactttat atggaccaag tcagaacgtt agttgatcgt
     7441 tatctttcac ctgtgataca aggagaaaag catccattgc ttacctcttc tatggtaaac
     7501 aattacgtta aactaggttt gataccagca ccagttaaaa aaagatataa caaagaacac
     7561 gtagcatttt tacttgcaat cactacgttg aaacaagtat tgactattcc tgaaatcaaa
     7621 gaagggatat tgttccaagg aaaaacagta ggaattcgtg aagcctataa tctattttgc
     7681 gatgaacaag aagcagctgt gtggatggtc agccaattag cacaaggtaa atctcatccg
     7741 caaaaattcg accaggcaac ccctgtagaa tatattgctg taagagcggc aactttatcg
     7801 tttgcaatga aacttcttgc ggaaaaaacg attgttctag aaaccgaata tttaaaagag
     7861 gagaaaacaa atgaaaaaca ataaaatcgc aattttagta gattcaggta cggatgttcc
     7921 agaagaattg ataaaaaaat acgagatgta tatgatccca ttgaaaatta tttataaaga
     7981 tcaggtatat acagataaaa ttgacattac ccctgaagag atctatcaac gtttgccaga
     8041 ggaaatccct ggtacatctt tgccggatgg tgagtcgatc acagaaatct ttgagcaaat
     8101 caaaaaagac ggttatgaaa aggtacttgc ggtaacaatt tccagtggat tgagcgggac
     8161 atataatgtg gttcgtttaa ttgcacaaca gcaagaagaa ttggaagcat atgtactaga
     8221 tacgaaaaat attggtattg gtgcaggttt ttctgcgatc caagcagcaa aatggctgga
     8281 agaaggagta gaatgggatc aactgatcag taatctgaat gagttagtcg aaagaacaaa
     8341 agttttcttt aatgttgcga cattggaata tcttcaaaaa ggtggacgga tcggtttagt
     8401 tgcttctatt gtaggaaccg ctttaaagct taacccaatc atctcatgta atgaagaagg
     8461 gatttattac acagtaggaa aagctagggg acgtaaaaaa agtttggata aaacggttgc
     8521 ttacgtcaaa gaaaaaatcg gtaatgcaaa ggtcttcaac ttagctgttg cccatgggga
     8581 tgcgaaagaa gaagcaattg aaatggaagc gagacttaaa agagagttcc cacaagcaaa
     8641 ccaaatctac tttggccaga tttctccggc attagtagtc cacactggtc cgggattatt
     8701 gggtgtcggt gtacaagttt tagaaggata aatcaaaaaa aggaaacact gaggtgaaaa
     8761 tcagtgtttt ccttttttat tgaagttaga acaaaaaata ataaagtatt ttgcactttc
     8821 attttaattt tctcttttgt tttataggca aagatgaaac aaaagcagaa atacggtata
     8881 atgagaaggt atgacaataa tatataagag gtggctcttt atgaaaaaag ttttagttgt
     8941 cgatgatgag aagccgattt cggatatcgt caaattcaat ttagccaaag aaggctatga
     9001 cgtttatact gcatatgatg gggaagaagc cttggaaaaa gttgctgagg tagaacctga
     9061 tttgatttta cttgatttga tgctacctaa aatggacggc ttagaagttg ctcgtgaggt
     9121 acgcaaaact tatgatatgc cgatcattat ggtaacagcc aaagattctg aaattgataa
     9181 agtcctaggc ttagagttag gcgccgatga ttacgtgacc aagccatttt ccaaccgaga
     9241 attagttgca cgtgtcaaag caaatcttcg tcgcggtgcc acggctgcga aggaaccaga
     9301 agaggcagca cctgcggaat tgacgattgg tgatttgacg atccatccag aagcttacat
     9361 ggtgacgaaa cgaggagaaa caatcgagtt aacacatcgt gaattcgaac tactctttta
     9421 cttggcgaag caccttggac aagtgatgac aagagaacat ttattacaaa cggtttgggg
     9481 ttatgattat tttggagatg tacgaacagt ggatgtaact gtacgtcgtt tgcgtgaaaa
     9541 gatcgaagat aatccaagcc atccaaatta tttagtgaca cgtcgtggtg tcggttacta
     9601 tttgcgtaac ccagaacagg agtaattgga tgaaaagaaa agtccgcttt tttcgatcag
     9661 tcaattttaa gatcgctatt acctttattc tgattttatt gatctcgatc gaaattattg
     9721 gtgcctactt tatacgtggt ttagaacgtt ccacgatcaa tacatttatt aaagatatga
     9781 atcaaacggt ggaatcatta gcaacaacaa tcagcccgga attgaatcga aaagataatg
     9841 cagatgatga agaggtaaat gcgaatatca aaagatttat tgaaaacagt gcgactagcg
     9901 atatcatcga aatacgggtc gttgacgaaa aagggatcat ccgaggaaca accgatgtca
     9961 atgaacaaag cgctgtggga aagaaaaatg attatgttga tatcaatgat ttcacgacta
    10021 aacgttatgt tgctttagat aacgacgata aacgggtgaa tatcaatgtt caaccgatcc
    10081 tgtccccgac cggcgatgcc gttatcgggg ctctttacgt caaaagtaat atcgaacaaa
    10141 aatattcgga aatcaataac acagcagtca ttttctttac tgcctcacta attgcggcat
    10201 ttatttcaat gatcgtatct gttttagttg ctcgttcgat tacacagccg atcggtgaaa
    10261 tgagagaaca agccattcga atcgctagag gagactacag tcggaaagtc aaagtgcatg
    10321 gacaagatga acttggacag ctggctgata ccttcaatca gttagctgaa cgtattgaag
    10381 aagctcaaga tacaatggaa gctgaacgca atcgtttgga tagcgtgcta tcccatatga
    10441 cagacggcgt cattgcgaca gatcgcaggg gtaaagtcat tacgatcaat gatatggctg
    10501 tttccttgct agatatcaaa aacgaggaag caatcggaca atcgattttg acacttttag
    10561 atatcgaaga agaatatacg ttgcggaaat tattagaaga tccaaacgaa atggtacttg
    10621 accgttcaac cagtacattg gaaagtgatc agatgatctt gcgagtagac ttttctatga
    10681 tccgtcgcga atcaggtttt atcagcggat tagtagctgt cttgcatgat gtgacagaac
    10741 aagaaaaaac agaacgggaa cgtcgtgaat tcgtctctaa tgtttcccat gaacttcgta
    10801 caccgttgac aagtatgcgt agttatatcg aagcattgag cgaaggtgcg tggaaagacg
    10861 aggaagtagc accaaatttt ttgaaggtaa cgctagatga aacagaccga atgattcgaa
    10921 tgatcaatga cctcttaaat ctatctcgca tggatacagg taatacccag cttcaattag
    10981 aatatgtcaa tttcaacgag ttagtgaatt ttgttcttga ccggtttgat atgatggtgg
    11041 gaaatcagga aaaaaattat aagatccgtc gtgaatttac gcaaagagat ctttgggtcg
    11101 aagtagatac agataaaatc atccaagtag tcgataatat catgaataac gcgatcaagt
    11161 attcaccaga tgggggaacg attacttgtc gcttattaga aacgcataat aacgtgattt
    11221 taagtatcac cgatcaagga ttggggattc ctaaaaaaga tctgaaccga gtgttcgagc
    11281 ggttctatcg agtagataaa gcacgtgcaa gagcacaagg tgggacagga cttggattag
    11341 ctatttctag agaagtgatt aaggcgcatc gaggcgctat ttgggcagag agcaaggaag
    11401 gaaaagggtc gactttttat atctcgctgc catatgaacc ttatgaggag gaatggtggg
    11461 aatgagaatc tctgaaaaag tcgttcgtat aggactcatt ttgatggttg cgttaagcat
    11521 atacttctct tatgcaatct ggctgagccc agcaggaaaa agttcaatca acgtagatga
    11581 gacaaacact caaatgatcg agtctcaaag caatcgaaaa acttccgaag tctttcttcc
    11641 gcttcatgtt acatggcacc attcgggaat gatccaagaa accaacagtg aaaatctcgt
    11701 gtaccgattg caattgatca tcgataaagc aagatttgga aagctttctg aagtagtttc
    11761 aggagacaaa tcacagtttg aacaggtgaa aaatctgaac tctggttttg agttggccta
    11821 taatgcgcct tttttgctaa gagaatacaa ggaagcattt gatttagatt tagatgtgtc
    11881 gaacttggca aaagaaagcg aaaatatgta ttttactaga atacaattcg atcaaggaaa
    11941 taaaaaaatc cgatttttga attattcgaa ccatctcgta tatgaagcaa atatttcgtt
    12001 gaattggaca gatatcaata aagaattaac agcaaaagac acaaaatgga cagaaatgac
    12061 aacagaacca tcgacgatcg acacgcaata tgctacgaaa cattcgatca aactgaagaa
    12121 gtatagttat atcctgtcac agcaacccta tacacttttt agaaatgctt ttttccagaa
    12181 accagatagc gtgaaaaata atgatgaaag caatgaattg attttttacg gtgggaatga
    12241 aacaatgtct atccattcgg aaacgcaagt agttgatttt cgtggagagc tgccagagaa
    12301 aaaagaaaat gctagtgtat tttctgaaag tttcccctat attagcaaat taggcagttc
    12361 tttagggaac ctcagatatt ttgaccgcag tgacaatacg atcgattatc ggatattcgt
    12421 ggaaggcttc ccggtttttg ggacagacag cagaggacag attggtgtgg aagttggcgg
    12481 tgaaacgaat ggttctgtgc aagtgaatat ccaaaccagt ttgaatacga ttcaagtgcc
    12541 gataccttca gacgaagaag tagaacttgc aagtacgaat gaaatcatcc agcagttaaa
    12601 agctaaggga gcagatagca acaagattgg ttcagtgatc gttggctata catggcaaaa
    12661 tgtcaaagaa acgaatcgtg tcgttgatct cttacctgaa tggtatgtga aatataaaga
    12721 taactggtat tcagctaacg accttctgga aaaattacct ggattggagg cgaattaatt
    12781 ggatttcaaa aaaatcgaat ggatattttt cgtcgctttt ttagggttga atgtgttttt
    12841 attcagcagt tatcatgacg cggtatacca agaaaacaat gtcattcgct ctaatcagac
    12901 tgaatcaatc caacaacgtc tggctagcga cgatatcact tacacgggga agatttcgtc
    12961 tgagaacaag caaggctatt atcttagcgc ggagcagacc aatcttagca cttcattagc
    13021 gaattatcgc aaggctggta aaagcggctt gctggcaagt ggtgtgtttt tagacggtga
    13081 tgttttgacg aaatctttat ccagttcaga tgctgaaaag aacgtgatcg atccagataa
    13141 aattagtagt tccttggaag attttttatc taacaaagac aatattttat tcggtaatga
    13201 ttatgtgtac acgaaaaact tttctcattt agaagaagaa ccactagaaa tccaagcaag
    13261 ccaagagtat gaaggcatcc cattcaatga tgatacagca aaaatcgttc tttctttaga
    13321 aaaaaacgat aatgattata gcatcacgaa atacatgcag acgcatttat ctgatatcga
    13381 gcagctgcgc gaaaagacgg aactgtatac ggaagaggaa gcaatcaata cattgtacat
    13441 caataacaaa ataccaagag gatcaaaaat tttatggcgt cagctagctt attcctgtat
    13501 tctgaaagtc cgggaaaaaa atgtgtatgt tcctgtttgg catgtagcga tcgaaacaag
    13561 cgataaagtg gtccaaattg agagcatcaa cgctttcagc aatacgatcg tgacaaacaa
    13621 tactgtacca aaagtggaag atcaataaaa aagcgagtat aatgaaaaaa gtttacaaag
    13681 taagaaggga agtaggagtc aaatggctga agaaaaacca gcttttaaaa tcagtatcct
    13741 agcaagtgga agttcgggta attcacttta tatcgaaagt gaaaaaaaac ggcttttggt
    13801 cgatgcagga cttagcggta aaaaaatcac ttccttgatg gctcaaattg atcggacacc
    13861 ggaccaatta gatggaatac ttgtgacgca tgaacatcga gatcatatac atggcgtggg
    13921 tgtcttggct cgtaagtatc atttggatgt ttttgccaat cagcagactt gggaagcgat
    13981 ggaaccactg attggaaatg taccagtgga acaaaaacat ctatttgaga tggggaaagt
    14041 cagaacattc ggcgatatcg atattgagag tttcggcgtc tcccatgacg cagcggctcc
    14101 gcaattttat cgtttccata aagaaaatcg gtcattcgtc atgttgacgg acaccggata
    14161 ttgcagtgat tatatgcgag gtgtcattga aaatgccgat ggttatttga tggaaagtaa
    14221 tcatgatctt gagatgttac gcatgggacc ttatccgtgg aatttaaaac agcgtatttt
    14281 aggagataga ggacatcttt ccaacgaaga cggtgcactt gtcatgactg atgtgatcgg
    14341 taatcggacc aaacgaatct atcttggcca tcttagcaag gaaaacaata tgaaagaact
    14401 tgctcattta acaatggaaa atgttttgaa agaaaaagat tttggcgttg gacatgattt
    14461 tgaaatctat gatacagatc cagatgaagc aactgagcta ttttcgattt aaacagttca
    14521 atcaataaaa atataaaaag cagaagataa aatcaatcag ggttggtttt atcttctatt
    14581 tttatttttt tagcatatta aaaaaataac cgtattatcg actgactaca ctgatgtgct
    14641 gcttttcatg tttctcatcc agtatctcat ctactaatgc aatcgcgtaa tctgccatgc
    14701 tgatcgtact gtcgctagca gcgtttcttt gtaaataatc atcacttaac aaataagtac
    14761 ctgtacgttt gccatgtgga tcaaataaag aagctggact gatataagtc caatgtagat
    14821 ttttagattg tttcaatttt aacaatcctt cgtacatatt gaaagctgtt ggatagaagg
    14881 gtgcattttt aggtacttgg tcaatcatac gagtcgtctt ttctgtatct aagaataacg
    14941 aagaggcacc cccaacgaca attaagcgtg tatcggttcc ttcaagcatc tcaatcaaat
    15001 gggacaaaga agtttgatgc atttcttctt ttcctttggg agcgttgaaa gcatcaacga
    15061 caacatcaaa agatgcaaga tcagtagttg tcaactcaaa taaatccttt tgtataaacg
    15121 gaacatctga tggcaatttt tctggatgac gcacgattgc cgtcacgtct aaatctctgg
    15181 acaatgcttc gtctaaaata aatgaacctg catggccagt agctccaatg agtgcaattt
    15241 tcataaaaaa cctccttttt tattcctgtc cattgtaagc agttgctgaa tattcgtcaa
    15301 agaatccgat ttttgaaaag ctcagtgcgc attcctttca tggttcgtga tcattgggta
    15361 tagttaaatt aagaaaactg attgataaaa atagaagttg aaggagataa gaaatgaaag
    15421 agtggttaga tcgtgccttt ttaccaatga taacatctgt tgtgaatctt agacccttaa
    15481 ctgctttgcg taatggaatg acttacacga tcccaataat tattgttggc tcgctgtttt
    15541 tcttatttgc tcattttcct gtggattaca tcgctcagtg gatagacaaa acaggatttt
    15601 ctatgtattt tacacaagta taccaggcta catttgatgt gatagccttt tggatcgttt
    15661 ttgccattgc atacgcttat gcaaaagaag aagacatcga tcgcttacca gtcggcataa
    15721 cagccttgtc tgcttatcta ctgcttattc agccactaaa tactcagata gatactgtct
    15781 ggacaggtgc tgaagggata ctaggagcag ttacaactgg gctcatcatc ggatggggct
    15841 atgcacgttt agtaagactt gcgaaagggt ggaagcaaag agagaagata cctgaaaatg
    15901 tttggcagtc ttttacttca ttgattccaa tcattgttat agtcactttt acgcttatcg
    15961 gttcagcggt ttttcaacat ttgttcagaa tgactgttgt tcaaggagta tggacatatg
    16021 tccagcttcc cattcaatcg ttcatagaca cgttgtttgg tgtgctttta ttaggttttt
    16081 ttgttccgtt tttatggtta tttggtcttc atggatctgc gatggtcaat gggttggtta
    16141 gtcccgtctt gcaagcgaat tcattagcaa atgcagaaat cttagcctca ggacaagagt
    16201 tgacggtggc aaatggcgga catatcgtta ctcagcaatt tctcgatcag ttcatgacgg
    16261 ttacgggtgc tggattgacg ttaggggctg tattttttat gacggttttt gcgaaatcga
    16321 agaaatacag agaattagga aatcttgctc ttttaccagc ctttttcaac atcaatgaat
    16381 caattatttt ttccacaccg atcgtgatga acccgatgat ggctgttcct tttattttcg
    16441 caccggtcgt ttcaggattg attacctatt ccgctttata ttttggattc gttcctttat
    16501 ttacagcagt ccaagtccct tggacaacac cgccgatcct ctcgggtttc ttagtaggcg
    16561 gagtgcctgc agcgatttta caaggtatcg tgttgtcaat tagttttttc atttatttcc
    16621 catttttgaa aaaaatagat tctgcaaact gcaaaaagga aagacagaca aaaaatgatg
    16681 gagcagcaga aaagatgaac gactaaaaaa caaaaatact cctgtcgctt tagattggga
    16741 aaatcaccaa tctaggatga caggagtatt tatagtcatg tttatactag ccctttttgg
    16801 acagctaatt gaacaagaac aactaatgtg ttcattccta cgtgtgtgat catcgatgtc
    16861 cagatccgac cagttgcttt gtactctagg ctgaagaaga aacctaggaa aaagtagatt
    16921 agcagatgac catcattatg agcaaacgca aacagcaaag agctgatcac tgctccgacc
    16981 caaaaatttg aatatcgact gatgatcccc agtatactgc ggcggaatac gaactcttcc
    17041 atgatgggtc cagcaacagt tgtcgccaaa ataaaagcgg gtgcttccat gatcatctgg
    17101 atgatatttt gtgtattctc ggaaggggtt gctttgccaa acaatacaga ttctaacatg
    17161 acagcgatgc tttgcagaat caatgcgaca aagattccaa ccaatccata aataacaatc
    17221 tttggagcag aagctttttt taccgctgat tcgatcgcta agggttcacg ttgcttaaaa
    17281 taaaaaatag ctaggacaac ggcgcctagt atatacatga ctgcagttgc tgtgatcaat
    17341 tcagacgtag attttacaag tcctatcgaa gctaggatca agggtgagat aaaaattaga
    17401 ccatagcaaa aaatgctgag gaagctatat ttttttaaag acatgtaatt cctcctttta
    17461 agggataaca agcatgcaag gcaagcttat atcgattgat ttcaattata tcataatttc
    17521 aataagcagc tgtcagaata aaatttcttt tctttgattt aaaagcttgc aaaaaagagg
    17581 gagaatgagt attattaaaa atgtaggtta gcactcgaag gttaagagtg ctaacagatt
    17641 aaaaaaagag ttatcggagg gatttatcgt gttaaaacca ttaggtgatc gcgtcattat
    17701 cgaagtcgca aaagaagaag aaaaaactgt cggaggaatc gttttagctt ctgctgctaa
    17761 agagaaaccc caaactggga cagttgttgc agtcggtgaa ggccgtttgt tagaaaacgg
    17821 tgaaaaagtt cctgccgctg taaaagcagg cgaccaagtt atgttcgaaa aatacgccgg
    17881 tacagaagta aaatacgaag gaaaagaata tctgatcgtc gcaggaaaag atatcatggc
    17941 gattgttgag taaatttgga agataaaaaa ataatcaaaa ataatcaatg aggtgaatca
    18001 atcatggcaa aagaattgaa atttgcagaa gacgcacgcg cagctatgtt acgcggggta
    18061 gataaattag cagatacagt gaaagtaaca ttaggaccta aaggccgaaa tgtcgtacta
    18121 gaaaaatctt acggctctcc attgatcaca aatgatggtg tgacgatcgc aaaagaaatc
    18181 gaattagaag atcattttga aaacatgggt gcaaaacttg tatcagaagt agcatcaaaa
    18241 acaaatgata ttgcaggaga cggtacaacg acagcaactg ttctgacaca agcaatcgtt
    18301 cgcgaaggtt taaaaaacgt aactgcaggt gctaatcctc taggcattcg tcgcgggatc
    18361 gaattggcaa caaaagcagc agtcgaagaa ttgcataata tctcaactgt tgttgattca
    18421 aaagaagcca ttgcacaagt agcagctgtt tcttcgggtt ctgataaagt tggacatttg
    18481 attgctgatg caatggaaaa agttggaaac gacggtgtca tcacaatcga agaatcaaaa
    18541 gggatcgaaa cagaactaga tgttgtagaa ggtatgcagt ttgatcgtgg ttacttatca
    18601 caatatatgg taacagataa cgacaaaatg gaagctgttc tagaaaaccc atatatcttg
    18661 atcacagaca aaaaaatctc taacatccaa gatattctgc cattgttaga acaaatcttg
    18721 caacaatcac gtccattgtt gattattgct gacgatgtag atggtgaagc attgccaact
    18781 cttgtattga acaaaatccg tggaacattc aacgtagtag ctgtaaaagc tcctggtttc
    18841 ggcgatcgtc gtaaagcaat gcttgaagat atcgctattt tgacaggtgg tacagtaatt
    18901 actgacgatc taggtttaga attgaaagat gcaacaatcg aaaacttagg aaatgcttct
    18961 aaagttgttg tagataaaga caatacaacg atcgtagaag gttctggaga aaaagaagca
    19021 atcgaagctc gtgtgcaatt gatcaaaaac caaattgctg aaacaacttc tgattttgac
    19081 cgcgaaaaat tacaagaacg cttagctaaa ttagcaggtg gtgtagctgt tgttaaagtt
    19141 ggtgcagcta ctgaaacaga attgaaagaa ttaaaattac gcattgaaga tgcattgaat
    19201 gctacacgtg ccgcagtaga agaaggaatg gtctcaggtg gtggtacagc tctagtgaac
    19261 gttatcagca aagtttctgc tgtagaagca gaaggagacg tggcaacagg tatcaagatc
    19321 gttgttcgtg ctttagaaga accaattcgt caaatcgctg aaaacgctgg ttatgaagga
    19381 tcagtgattg ttgataaatt gaagaacgtg gaactaggca ctggtttcaa tgctgctact
    19441 ggagaatggg taaacatggt tgaagcaggt atcgtagacc caactaaagt aacacgttct
    19501 gccttgcaaa atgccgcttc cgtttctgcc ttgttattaa cgacagaagc agtcgttgca
    19561 gataaaccag aaccagcagc gccagcagct cctgcaatgg atccatcaat gatgggcggt
    19621 atgatgtaag aaaaagcaac ttgcttatct acaactgatc attagtgaaa gacagataaa
    19681 aagaggctgg aacagaagcg tttaactcca agaaataaga agaaattcac ggaaattgct
    19741 tttcaaattt ttgtgaattt cagcttattt ccgaaggagt tgcttctgct tccgctgttt
    19801 atacgtgttt agagcgtgag acaaaag
//
LOCUS       DACEKH010000050        19452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEKH010000050 DACEKH010000000
VERSION     DACEKH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.051"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            133..3660
                     /locus_tag="IW662_001639"
     CDS             133..3660
                     /locus_tag="IW662_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0866465.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IW662_001640"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IW662_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ0866466.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IW662_001641"
     CDS             7448..7789
                     /locus_tag="IW662_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0866467.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IW662_001642"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IW662_001642"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0866468.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IW662_001643"
     CDS             9146..11566
                     /locus_tag="IW662_001643"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ0866469.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKEINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IW662_001644"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IW662_001644"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ0866470.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IW662_001645"
     CDS             13000..13737
                     /locus_tag="IW662_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866471.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IW662_001646"
     CDS             13751..14566
                     /locus_tag="IW662_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0866472.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IW662_001647"
     CDS             14579..15226
                     /locus_tag="IW662_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0866473.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IW662_001648"
     CDS             15242..15898
                     /locus_tag="IW662_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0866474.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IW662_001649"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IW662_001649"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ0866475.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IW662_001650"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IW662_001650"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ0866476.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IW662_001651"
     CDS             18240..18758
                     /locus_tag="IW662_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0866477.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IW662_001652"
     CDS             18865..19365
                     /locus_tag="IW662_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0866478.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6539 a         3340 c         4176 g         5397 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaaga
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga ca
//
LOCUS       DACEKH010000051        19401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEKH010000051 DACEKH010000000
VERSION     DACEKH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.052"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            168..362
                     /locus_tag="IW662_001653"
     CDS             168..362
                     /locus_tag="IW662_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866479.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IW662_001654"
     CDS             631..930
                     /locus_tag="IW662_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866480.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IW662_001655"
     CDS             complement(1027..1461)
                     /locus_tag="IW662_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0866481.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IW662_001656"
     CDS             complement(1693..2403)
                     /locus_tag="IW662_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0866482.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IW662_001657"
     CDS             complement(2393..3253)
                     /locus_tag="IW662_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ0866483.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IW662_001658"
     CDS             complement(3258..3896)
                     /locus_tag="IW662_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ0866484.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IW662_001659"
     CDS             complement(3911..4210)
                     /locus_tag="IW662_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0866485.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IW662_001660"
     CDS             complement(4253..5737)
                     /locus_tag="IW662_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0866486.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IW662_001661"
     CDS             complement(5757..6218)
                     /locus_tag="IW662_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0866487.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IW662_001662"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IW662_001662"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ0866488.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IW662_001663"
     CDS             7521..8291
                     /locus_tag="IW662_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0866489.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IW662_001664"
     CDS             complement(8564..9391)
                     /locus_tag="IW662_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0866490.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IW662_001665"
     CDS             9959..11194
                     /locus_tag="IW662_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0866491.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IW662_001666"
     CDS             complement(12193..12453)
                     /locus_tag="IW662_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0866492.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IW662_001667"
     CDS             complement(12480..13781)
                     /locus_tag="IW662_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0866493.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IW662_001668"
     CDS             complement(14073..14531)
                     /locus_tag="IW662_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0866494.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="IW662_001669"
     CDS             complement(14544..15869)
                     /locus_tag="IW662_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ0866495.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="IW662_001670"
     CDS             complement(15886..17298)
                     /locus_tag="IW662_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0866496.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="IW662_001671"
     CDS             complement(17315..18079)
                     /locus_tag="IW662_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ0866497.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="IW662_001672"
     CDS             complement(18183..19058)
                     /locus_tag="IW662_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0866498.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="IW662_001673"
     tRNA            complement(19313..19397)
                     /locus_tag="IW662_001673"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
BASE COUNT         6171 a         3989 c         2986 g         6255 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata tttgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa t
//
LOCUS       DACEKH010000052        19298 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEKH010000052 DACEKH010000000
VERSION     DACEKH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.053"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            958..2208
                     /locus_tag="IW662_001674"
     CDS             958..2208
                     /locus_tag="IW662_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ0866499.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            2227..3237
                     /locus_tag="IW662_001675"
     CDS             2227..3237
                     /locus_tag="IW662_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ0866500.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDVHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFEDGGTRVIHYTPETYIEKQ
                     NG"
     gene            3247..3900
                     /locus_tag="IW662_001676"
     CDS             3247..3900
                     /locus_tag="IW662_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0866501.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3919..4917
                     /locus_tag="IW662_001677"
     CDS             3919..4917
                     /locus_tag="IW662_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0866502.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWKPTVSLEEGLRESYNWIEKQIKG"
     gene            4993..5754
                     /locus_tag="IW662_001678"
     CDS             4993..5754
                     /locus_tag="IW662_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ0866503.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5772..6878
                     /locus_tag="IW662_001679"
     CDS             5772..6878
                     /locus_tag="IW662_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0866504.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRDVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            6868..7101
                     /locus_tag="IW662_001680"
     CDS             6868..7101
                     /locus_tag="IW662_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866505.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            7300..7755
                     /locus_tag="IW662_001681"
     CDS             7300..7755
                     /locus_tag="IW662_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAQ0866506.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            7769..8542
                     /locus_tag="IW662_001682"
     CDS             7769..8542
                     /locus_tag="IW662_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0866507.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            8529..9659
                     /locus_tag="IW662_001683"
     CDS             8529..9659
                     /locus_tag="IW662_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0866508.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            9690..10925
                     /locus_tag="IW662_001684"
     CDS             9690..10925
                     /locus_tag="IW662_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866509.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFVPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLSVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            10950..12059
                     /locus_tag="IW662_001685"
     CDS             10950..12059
                     /locus_tag="IW662_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866510.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAKYEKYMDFVWEE"
     gene            12101..13435
                     /gene="gmhB"
                     /locus_tag="IW662_001686"
     CDS             12101..13435
                     /gene="gmhB"
                     /locus_tag="IW662_001686"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAQ0866511.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            13470..15107
                     /locus_tag="IW662_001687"
     CDS             13470..15107
                     /locus_tag="IW662_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ0866512.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            15209..15604
                     /locus_tag="IW662_001688"
     CDS             15209..15604
                     /locus_tag="IW662_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ0866513.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            15622..16353
                     /locus_tag="IW662_001689"
     CDS             15622..16353
                     /locus_tag="IW662_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866514.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVGRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            16377..17828
                     /locus_tag="IW662_001690"
     CDS             16377..17828
                     /locus_tag="IW662_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0866515.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            17854..18465
                     /locus_tag="IW662_001691"
     CDS             17854..18465
                     /locus_tag="IW662_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0866516.1"
                     /translation="MIDIISAIMVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            18647..19162
                     /locus_tag="IW662_001692"
     CDS             18647..19162
                     /locus_tag="IW662_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAQ0866517.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNGERLSF"
BASE COUNT         6219 a         2696 c         4218 g         6165 t
ORIGIN      
        1 gtttagacat tttcctctag gaaaaaatta actagcacca cgggtgatat atatgtatat
       61 ggtgactgaa gacaaaaaaa cagaactaat gaaactgaat aaaaatatag agtgaactag
      121 tttctaagag ggattttaaa ctagatttca ttggtaataa aatttggaga gatggaaaaa
      181 cagcacttaa ggaagcagat tttgttgtca tttttgttcc tacaaactat ggtagtgaat
      241 gtgttattga attagtgatg tctgttattt cagaggcatt ggggataatt aaatccacta
      301 tacctgtagg atatacaaaa agtggaagaa aaaaatatta tcaatagatt ttatttagtt
      361 cagaattttt acgtgaaagt agattattaa tgataatctt tatccaatta gaattattat
      421 cgtaggatgt gatgatgaaa gccgtgaaat tgttcaagta tttaccaatg tctccctgcc
      481 agtaaatccg cttagaattt gagccttgaa aatgtgcaat gataaaaaac aaaaactcgc
      541 tcaaaataga cagtttgggg tgggaataga ccaataatgt accagacaaa cttccatttg
      601 gggtgaaaat aggtcaataa agcgatgtta aacatacctt tggagtaaga atagagggta
      661 ataagtgaat ttataaactt tttggtgact ggttgagtgg aaaatgtagg tacatttcaa
      721 tgcgaaaatg tattataacc ccaaacgagg tcaataaaaa tgactcaaac ttgcaatata
      781 accctaaacg ggtaggggat ttatgaaaaa ttgaatttgc cggcgtagga gctggctgtt
      841 ttaagtatga atgaacacag ctttcgggct gttttatata aatagcaggt tactgttcac
      901 aaacagcatt ttgttaagaa agtagtgacc acagcagtgg tggaaagagg aaatattatg
      961 aagaaacagt ataacattgc agtcgccggc accggttatg ttggtttgag tattgccact
     1021 ctgttggcac agcaccataa ggttacagcg gtggatatca ttccagagaa ggttgaactg
     1081 attaataacc gtaaatctcc aatccaggat gattatattg aaaagtacct ggctgagaaa
     1141 gaactgaatc tgaccgcaac attggatgca aaggcagcat accagtctgc ggattttgtt
     1201 gttattgcgg ctcctactaa ctacaatagc cgcactcaac attttgacac atccgctgtt
     1261 gaggctgtta ttaggctggt catggaagta aatcctgatg ccattatggt tattaagtcc
     1321 accattcctg ttggatacac cgcttctgtt cgtgtaaaga ctggtagcaa gaatattatt
     1381 ttcagccctg agttcctgcg tgaaagtaag gcactgtatg acaatctgta tccttcgcgt
     1441 attattgtgg gtaccgatat ggatgatgag cgtttggttg aggatgcaca cacttttgct
     1501 acactgctac aggaaggcgc aatcaaagag aatgtggaca cattgttcat gggctttact
     1561 gaagcagaag ctgttaagct gtttgccaat acatatctgg ctcttcgtgt cagctacttc
     1621 aatgagctgg acacttatgc agagatgaaa ggcttgaaca ctcagcagat cattaatgga
     1681 gtttgccttg atcctcgtat tggtactcat tacaacaacc cttcctttgg ttacggtggc
     1741 tattgcctgc cgaaggatac caagcagctt ctggccaact acgcagatgt tcctgaaaat
     1801 ctgatcgagg caatcgttga atctaaccgt actcgtaagg actttattgc ggatcgtgta
     1861 cttcagatgg ctggttatta caattacgca gaagcaaatc agtatgatgc tggcaaagaa
     1921 aagcctgttg tgattggtgt ttaccgtttg accatgaaat ccaattctga taacttccgc
     1981 cagagttcta tccagggcgt tatgaagcgt gtaaaagcta aaggtgcaac tgttgtgatt
     2041 tatgagccga ccttgaaaga tggagagact ttctttggca gtaaggttgt gaatgatttg
     2101 gaacagttta aggcaatgtg tgattctgtt atagcaaatc gctacgatgc ttgccttgat
     2161 gacattaagg aaaaagtata tacccgtgat atcttccaga gagactaaga ttaggagaga
     2221 gtatttatga ttattacaaa aacaccgttt cgtatgtctt tttttggcgg tggaacggat
     2281 atggaggatt atttcaaaga gaatggcggt gcagttcttt ctactacttt tgataagtac
     2341 tgttatgtga atgtgagaca tctgcctaga ttttttgatt acagcgctga gttatcttat
     2401 tctcgtactg agagagtaac tagcattgaa gatattgagc atcctgcgat tcgtaatgcg
     2461 atgaaaatgc tcgatgtgca tgaaattcgg cttacatacg aagctgattt acctgctcgc
     2521 tctggtctgg gtacatccag ttcttttgca gttggtatgt tgaatgcttt ttatgcactg
     2581 gagggtaagt atgctgacaa gaagaaactt gctgatgaag ccatttatct tgaaagagtt
     2641 ctgtgtaatg aagccggtgg atggcaggat cagattgccg catcttatgg tgggttcaat
     2701 cgaattaatt tcaatgtgga tggttatgag gtgctgcctg tcattatttc gcctgaacgt
     2761 aaaaagcagc ttaacaacaa tctgatgatg ttctttaccg gattcactag attttcttct
     2821 gatgttcaaa aggctaacaa cgtcagtggc acagaggata agagagtaag actgaaaaag
     2881 atgtatgagc ttgtagatga agctgaggca attcttactg ataagaacag aaatttagat
     2941 gatttcggta gacagcttga tgttacttgg agattgaaaa aaggtactgg tggagcaatt
     3001 agtacaggaa gcattgatga actatatgaa aagggtatgg ctgcaggtgc tttaggcgga
     3061 aagttgctcg gtgcaggtgg tggaggtttc ttggttttct atgtacaacc tgagaaacag
     3121 gatgcagttc gttgggctat gcgtgatttg atgtatattc cgtttgaatt tgaagatggt
     3181 ggaactagag ttattcacta cacgccggaa acttatattg aaaaacagaa tggataagga
     3241 gtaacaatgt tagcggaaaa acttaataaa catattgact tattgattaa acgatatcct
     3301 acacttgaga cgattaaaca ggacattatc gatggatacc taattatgga ggaatgctat
     3361 ttgaatggtg gtaaacttct tgtggcagga aacggaggat cagccgctga ctcggaacat
     3421 attgctggcg agttgatgaa gcggtttaag acgccacgtc ctgtaagtaa ggaatttgcc
     3481 gataagttag ttgcaattga ccctgtaaga ggtccggaat tagccaaaaa tctcgagtgt
     3541 ggtttaacgg caattccttt ggtcgcacac gaagcattgt ctactgctta tattaatgat
     3601 gtcgatggtt tgggtgtctt cgcacagcag ctgtttggct ttggtagatc tggtgacgta
     3661 ttccttggta tttcgacgtc cggaaatagc aagaatatta tgagcgcaac tgttgttgct
     3721 agggctctcg gtataaaagt tattggcttg acgggcgcaa agggtgggga acttgccacg
     3781 gtagcagatg tttgtgttaa ggctcctgaa gttgaaacat acatgattca agagtatcac
     3841 ttgccgatat atcattgttg gtgtttgatg ctggaagata aattctttgg aaacgaataa
     3901 aatttggagg aatgttaaat gaaaaagaca attatggtta ccggtggttg cggtatgatt
     3961 ggctccaatc ttgttaagag actggtgaag gaaggctgct gggatgttta tgtagctgat
     4021 aatctgtgga gaggaaagtt ggagtacctg aacgacgaag atgggcatcc tgtcatcgac
     4081 ctggatactc atttcttcaa tgctgatctg actgattatg agcaggctaa aaaggttatt
     4141 ggtacgactg agtatgtagt tcatctggct gatgttgtgg ctggcattga ctatgtgttt
     4201 aagaaccagg gcgaattgtt ccgaatcaat aatctgatca attctaatgt ttttgattgt
     4261 tgccgtaagg ctggcaagga gaaaatcaag ggtgttatct atgttggtac cgtatgctcc
     4321 ttccctctga ccagacagaa tactttgaat cctgagcctc tccgggaaga ggagttgttc
     4381 cctgctcttc ctgagtctgc ttatggctgg agcaaactga tgggccagtt ggagatgggg
     4441 tatttggaaa aagaaaccgg aattccgtgc tgtatcctga tgcttcataa tgtttatggc
     4501 acacctactg attttggcga acgtagtcag gttattcctg ctctgattcg taaagcaatc
     4561 aataatcctg aagagccttt taatgtgtgg ggtagcggag agcagggacg tgctttcatt
     4621 catgtcaatg atatcgtaaa tgcattggtg ctcgccctgg ataagggttg gggtcatggt
     4681 catattcaga ttggcccttc tcactgtact tccatcaagg aaattgcata taagatcatt
     4741 gaaatttctg gcaaagacat caagcctttc tttgatactt ctaagcctga aggtgataag
     4801 gctcgttgcg cagattattc taaggcgaaa gagattttgg attggaagcc tactgtttct
     4861 ttggaagagg gtcttcgtga atcctacaat tggattgaaa agcagattaa gggttaataa
     4921 gtatataagc tgttcttcct ccgtgtaatt agaggatgcg gaggaagaac atatgagtaa
     4981 ggatgtactg agatggaaaa gaatatgtat aaaagaaaac gtgatttcac tgtgtgtgga
     5041 atagtaggtg tttgtgctac tacaaagaac gagtttgtaa gggaagtttt ttccatgcca
     5101 acatctccgg taagtgcgac tgttgatctc gtgggagtgc cggccattat ttcggcaaag
     5161 gaaaatccca aaattgctga gatgtatagc caggctacaa tggctgcaat agatggtatg
     5221 cctattgtta aaattggacg gcgcaaagga tttacctgtg aacgatgtgc tgcaccagat
     5281 attatgggaa tggtttttga agaaggcgtt agacagggaa aaacacatta tttctatggt
     5341 ggaaaagata acgaagtgtt aaataagctt cgtgcaaacc tagaaagaga ttacccggga
     5401 atcaaaattg ttggaatgta ttccccacca tttcgtccgt taaatgaaga agaggatagc
     5461 aagatttgcg aggaaatcaa caacttaaat ccagattttc tgtgggttgg tattggtgcg
     5521 cctaagcaag aaatgtggat gtgggaacac cagaagaaga tccataacac agtaatgttc
     5581 ggtgtaggcg ccggctttaa cttttttgcg ggcactttgg aaaaagctcc tgaatggatg
     5641 gaaaaagcta gtctagagtg gttatttaga ctggtaaagg aacctcgtag actatggagg
     5701 cggtatatct tgggaggtat aaagtatatc tactatacta ttgaatctaa gtaggggggc
     5761 aataaaaata gatgaaaata cttatagtag ttacaatgac gaataacttt ggtaagaagg
     5821 ggtcctataa ttctcaggaa ataggattag ctcgagggtt agctgaatta ggccacgaag
     5881 ttattgtata taaaagtgta aaaagtgatg aggaagttgg aacagagcaa ttaagcaata
     5941 cggcgatgat tcactatatc aagtgtcgtt atgtaggatc aaatggattc ttatcaacaa
     6001 agaattttga taagaatgcg gatgttctaa tacaatttgc ggaccttcaa ctatcagtac
     6061 ctcatgtata ctcatggact gtaaaaaatg gaataaaata tataccttat aatgggataa
     6121 cgcgaagtca aagtggcaat aaatatataa agacaattat tgaaatattg gcgcatcgaa
     6181 attataaaat ttataagaaa aatgggtgtg ttgccaaaaa tgatcgagtt tctgaaaaat
     6241 tagtacaatt aggagtaaat aaagtgaaac ttgctcctgt ttgtgtggat tttgaacagt
     6301 tacataagaa ttacgaagca atagatttta ctactctcag agaaaaatgg aatattcctc
     6361 gcgatgttcg tgttatttta tatgtaggac ggtttgaaaa ggataaaaga ccgttagata
     6421 tgattagttt atttaataaa cttgatgagc ataattattt gctgatggta ggaactggtg
     6481 ctttactcga gcaaacgaag cagcagataa atttacttgg actgcagaat agagtaaaaa
     6541 taattgagcg tattcctaac aaagatattt gggagttata ttgcttatcg gatgtatttg
     6601 taaatctaaa ctatactgaa atttggggta tggttttgct agaggcaatg tactatcgcc
     6661 tcggaattat tgctgttaat gcacctggcc cacagaatat tattttggat aatagatatg
     6721 gaatgttggc taaatctgac gatgaaattt tgacattctt aaaagacgga cgatttacca
     6781 acgatatcat agatgcagca tatcagcgtg taactacaga atttagatgg tcaaagtcgg
     6841 gagaaattat gcttagcatg ttggaaggtg ataaatgata tggaagtaac gaaattaatg
     6901 gcattgagga atcgatatgc tttaaacata gttgataatt gcacgagaaa aatagccaaa
     6961 attcttggtt gttgtattgg taaaggtgca cagataggga actctgttga atttgttcat
     7021 aattctgtag gcactgtgat tcattctgac acaatccttg aagatggtgt taaagtttat
     7081 cagaatgtga cgtgtggttg agcagatagt aatgaacgta tcatcattcg agtaaaaaag
     7141 aacgctgttt tatgtgtggg tgctaaaatt ctttgtaaat ctaacacaga attatgcgtt
     7201 ggggaaggtg ctgtagttgc tgcaaataca gtggtattga ggtctattcc accctacgaa
     7261 gtgtgggggg tacctgctaa aaagatgggg agataaaaca tgaatgcata taaattatac
     7321 aaagtagaac gatggtttta cttacacaaa atggagtttt ttgccaagat tgtaaaggca
     7381 ctaatttatg tacttcataa tagctcaatt tcatatcaaa cagaaattgg agataattgt
     7441 aagttcttat acggtggcat aggttgtgta attggaaaag atacaaaaat aggtaatcac
     7501 gttataatcg gaacgaatgt actgactggt ggtagatcca ataagcctgg tatgcctaca
     7561 attggaaaca atgtatatat cgctactggc gcaaaaattt taggtaatat cacaataggg
     7621 gacaatgtaa tagtaggggc taatgctgtg gtcattaatg atgttgaacc taattgttcg
     7681 gtaggaggcg ttccggctag agtattgaaa aaagatattg atgtaaatga ttattgtagt
     7741 ttgaatcgtt actaaaagga gagattgtat gttgtatttt gttattcctt taatgagtgc
     7801 gaaggcagca aacaattggg agatggttag taccttattt aaccgaacat tatggtcgtg
     7861 ttataatcag actgatcctg atttcaaaat cattgttgct tgccatgaaa ttcctaaact
     7921 cacgcatgaa tatgatgaca gagttgaatt tattcaagtt agcgaaaagg atgcccctat
     7981 tcccacaaat caagacgaaa aaatgattga taagggttat aaaacacatg ctctcgcaat
     8041 gcgtttgcga gagttgggcg gcggtttcgc tatgatggtt gacgcagatg atcttgtaag
     8101 ttctcatttg gcggaatatg tgaattccca tccccaggaa aatggatttt atgtcaaaac
     8161 gggatacgtg tactttgttg gagacgatta tatgaaggtt ctaccaaagt tctcatcagg
     8221 aagtgcgtgt atagtaaaat atgcagtgga agacctgcct gaggcatatc cggaagttat
     8281 gacagcaaat tgtgatgata atccatggat cattagaaaa cgacatgggg gcgttgttcc
     8341 agcatgtgaa gagcaagggc gtccattaaa acctcttcca ttcaaagcag cagtatatgt
     8401 attaggaaca ggggacaatc actcgcttta tggtaaaacg acaaaatatc agacgaagat
     8461 gcgagagata ctggaaatgt ttatagcaaa acgtaaaatt aaaggaaaac tgaaagcaga
     8521 attttctgtt gattggttat aaggtgagta ttatgaaggt attgatgtgt gaaagccaaa
     8581 tgccagagaa ttggtattcg tttgatcttt ctaaagaact taatcaatat gttgatttaa
     8641 cattgctgtg taagcgtgat gttcctcctt tagatcctaa aattaagagc aaacatattt
     8701 tgtatcataa aagtacaaat aaagtgtttt cggcacttgt ttttctgaaa agcttgttct
     8761 taatttggaa ggaaataaga tttggtgatt atgatattgt tcatttgcaa ttgatgaaaa
     8821 gtctcaaata tgaaggcgga ttgtacgttt tatgtaagag acactataaa aaattaatta
     8881 tgacagttca taatgtgtta ccacatgagg ttcgtgaaaa ggatcgccaa gagcacttta
     8941 atatatattc aaattgtgat ggtttcattg ttcacaatcg agtaacagaa cagcatttat
     9001 atgctgaatt cccagaatta aatgcaaagt catatgtttg tccacatgct atctatggag
     9061 atgttaaaca tatatctaga gcgtatgata aagataagtt tgaatttatc ttgtttggac
     9121 aaattcgtcc atataaggga attgatgtac tgcttaatgc aatcacagaa cttccaatgg
     9181 atgtaagaaa gaaagttcat tttaccatag caggtcaaca acatcctaaa caggatagta
     9241 ctgactataa aaaaatgatt gaagatttaa aggtttcaga ttgtgttcta tataagccag
     9301 agagaatttc tgatgaagaa atggacagta tgttcagaaa tgcagatgcc gcaatatttc
     9361 catataaaga aatctatgga agtggagcgc ttcttatggc atacacttat tgcttaccaa
     9421 ctattgttag taatgtacct gcttttatgg aagaaaccga ggaaggaaag atagggttac
     9481 ttttcgaaaa tttgaattct aagagcctag cagacacaat tgtaaagtat gtgaacctta
     9541 gcccagctga gaaaagcaaa atagaggatg atatatatga gcttgtaaac cgcaagtata
     9601 cctgggcggc atctgcaaaa ctcacatacg aggcatacaa acaaattttg aatgagtagt
     9661 aaaacaatta gaaaggaata ataattaaca tgcagattag taaatggaaa atcatacagt
     9721 ttctaatggt ctttctaatg attatctcat caagcatatt gaagcctttg ggcggctggt
     9781 tgggagaaac gatgaacgtt agacttgtga cagtcattct agcaatagta tgtttgttaa
     9841 tagcctcatt accagcattt agaaaaaata gatatttaaa tttctgcact ttactgattt
     9901 gtgttgttat aatagcagct tacatctact cagataagac aatatgggtt aaatctaggg
     9961 aaagtatgag tttatactat gtattattgg cgtatccatt gttctgtact ttaattaacc
    10021 aagcgtggaa tttggattca atgctaaata cgatatgtgg tgtaacattt ctttcataca
    10081 ttcttagaac atctatttca cttattcaga aattttcagg agtacttctc tatgaaaaca
    10141 tatatcgtga gtgtgccccg gaaaattggt atcgtggagg ctttttaaga attaatccac
    10201 catgttttac aattataata ataccgattg ctttgtatat gattgagcga caagttgtag
    10261 ctagaagaaa agtaaaatat tatttactga ttgctagcgc actggcatat tctgctgtta
    10321 ttcatggttc tagatctgtc cttgtctatc aaattattgt acttggttgc tatatccttt
    10381 ttaagaaagg ttcttcgaaa agaaaaattg caatgtgggt tttagctggg ttgcttgtag
    10441 tcatatttat taattcgacc atgttctcaa catttgttga aactttcaca aacagtacag
    10501 gtgaatatgc aggatctgct gaatctcgat ttgcttcagt ttggtttttt gttcctaaat
    10561 ttttgcaatc gcctttgttc ggtactggta ttcttactgg tgaggaattt acgtttatat
    10621 taccaggagg agttagagga cacctatctg atattggatt tttctataca attactcaat
    10681 atggtgtagg tatacttata tttgatattc tgtttattat aaaagggttc aaaacagcat
    10741 tacttgcatc aaaagtgggt atgactggct atcaatattt agcatttgga attacattaa
    10801 gtgttttgct aacagggatt aatatagact ggttttatcc gatatttact ccagctattc
    10861 ctgtatgctt agcggttata gaatacatct atcaagaatg tagaagtgtg gctaacatag
    10921 aataggaaat attaatagga gtgcatctca tgaataagaa agagatttta tataacatat
    10981 tgaagaagta cgaaaattta ccatatgtgt ttaggaacat gtttggtcat aatataagta
    11041 aactcgtatc ttggaaattg actgctgatt gtgcaacagt agaagaaaat tctgaagttt
    11101 atgcagcgtt tcaattgcgc tcaaaggaca tgtctgatgt tccagtgatg ggtttagtta
    11161 attgttgcaa agatgtagca attgtaatgc aggggccact atgtttaaag gatgatttct
    11221 cttataatac tctggcctac tataagaaat gttatccaaa tgctttaatt attctttcga
    11281 catggaagga cgaagatatt aaggcattga aaaggataga ggatcttgga gttatagtta
    11341 tttgtagtga gaaacctgaa aagcctggac acttaaatat taattatcaa gttggaaaca
    11401 ctttgcctgg aatagtcaga gcaaagcagc ttggtgcaaa gtatgtttgt aaaactagaa
    11461 cagatcaaag aatataccat ccaaatgcaa tggcgttttt ctgcagttta cttgaacagt
    11521 atcctgttaa taacgaagat gattggaaat taatacaaaa tcagcgatta ctgttactat
    11581 caatgccgta tggagacatg tttttcccgt attgtttgtc agacttcctg tattttggtg
    11641 atgttgatga tatgataaac ctcttctcga ttcctagtga tgtgagagaa aagggagctg
    11701 tgtctagagg agtctcaaga agggagatat ctgaaaataa tttagctcca gaagttcagc
    11761 ttcttcgaag ctatatttca cgaatggggg gcaatgagga gtgctctatt agggcatatt
    11821 gggagtttgt aaaaaaccat gttattacta ttaacaaaaa tcaaattgat ttgtactggc
    11881 ataaatatgt aggtcgttat tcaaataata ctatttatgg tacgtattat attgatgata
    11941 gccaagatgc acttcactgc tataattttg attttataaa ttggttgaat ttgtacactg
    12001 gaaaatacga atacaaagca aaatatgaaa aatatatgga ctttgtttgg gaagagtaat
    12061 taacttttat agtaaaaagt actttaggga agggcatatt atgaaaacag taattatggc
    12121 gggcggacga ggaagccgaa tttcggaatt gtttccggat attcctaagc caatgattcc
    12181 tatagatggc attccagttt tagaacgaga attgtgttcg cttagagatc aaggctttaa
    12241 tgatattatt ttgactgttt cctatttgca tgaaaaaata gaggagcatt tcggagatgg
    12301 ttcaaaatta ggaattaaaa tcaaatattt tattgaaaaa actccacttg gcaatgcagg
    12361 tgcattgtat tttttgttgg atgaactggg tgatgaaccg tttttacttt tgaatgctga
    12421 tgcagtattt gatgttgatt tcaggcagat gattagattt catgttgaga ataacgcaga
    12481 tgttactttg tttacgcatc ctaactcaca tccgtatgat agtggattaa ttatatccga
    12541 tgacaataag aaagtgcttc aatggttagc gaaggaagat caaagacctg aatattataa
    12601 aaaccgagta aatgcaggtc ttcatgttat aaatccgaag gtgttgagag aaaatccatt
    12661 aatggaaatt gtaaataaat caatagttga aggtgggggg ctaccataca aaattgattt
    12721 agatagaatg attctgaaac cattggctaa cacagggcgt atgtattgct acgattctcc
    12781 agagtatgtt aaagatatgg gaacaccgga ccgatataga gctgtaatta aggactttca
    12841 aaagggaatt attgttggta aaaatctatc caatccacaa aaagcaatct ttttagatag
    12901 agatggaacg attaataaat acgttggttt tttgagaaat gcagaagaat ttgagttaat
    12961 tgatggagta tcggaggcaa ttaagattat taattctcta ggttttctgt gtattgttat
    13021 aacaaatcag cctgtaattg caagaggtga agttactgtt gatgagctta atagtattca
    13081 caataagctt gaaacaatgc tagggcatgc tggtggattt ttaaacggta tttattactg
    13141 tccgcaccat ccccataaag gatatgcagg agaggttata gaattaaaaa ttgattgcaa
    13201 ttgtcgcaag ccaaaacctg gactgttatt agaagcatca aaggatttta acataaacct
    13261 aagtcaatca tttatggttg gagatagtga aagtgatgta attgctggaa aaaatgctgg
    13321 ttgtaagaca gtactgatta cagaagacat caccttgcct gctaattatg gccaagacta
    13381 tagtgtctgt tcattgttag aatttgttaa aaatcattta tgcactatgc aataattggt
    13441 ttagtgaaac ttgtaaaagg agtaattaga tgaaggtatt aattccagca gcaaaagttg
    13501 ttgaaaaaga attgcagagt ataggaatgt taccagcaat tatctatcca attaataaaa
    13561 aaattgtctt tgattatttg tataatcagt ataatgcaat ctgcgattca ttggatatta
    13621 tctgttatga aaaagcggat aaggttcata gaagactgaa aaactatcat gattccagaa
    13681 ttcatatatg tgaccttcct gaattagggg atctagggca aaccgtgtat tttgggttat
    13741 cagatgaaga tgagcctgtt ctaattaact tcgcagatac aattgttatg gagagtatgt
    13801 ctgatataga aatggataca ttctattatt ctgaagattt tgtgtcgagt acttggacat
    13861 actttaaaat taatgaagga ataattactg atatccacga taaagaggaa gcttacgtat
    13921 ccgataaacg tgaaaaatta tttattggcg tatttatgtt ttcttcgtca aaacttctga
    13981 agagttgctt aagagaggca gttgagagtc ggaaacaggg gggaataaac tcattttatc
    14041 gggctttaca gctatacagc gaacgttgtc cgctaaaggc cgtaagaaca gaaaagtggt
    14101 ttgatattgg acacatcgat aaatattaca atacaaagtt ggaagttaaa gcacgagagt
    14161 ttaatcatat ctctatagat aaagatcgag ggatattgaa gaaaactagc gatgacaagg
    14221 ataaatttat tggagaaata aagtggtatt taaaacttcc tgctgatatt gaatatgtaa
    14281 gacctagaat ttttgattat tctacatctt acgtagctcc ctatgttgat atggaatatt
    14341 atgcatatca tacggttcat gagttatact tatatggtga tttaaaatat aaccagtggc
    14401 atgatctgtt tagccgtata aagtttatct gtagcgattt taagcgatac accttaaaag
    14461 atgaaaagat tagagagtca ttggaagata tgtatttgcg aaaaaccatg cagagatttc
    14521 aaaaattgtc tgaagataaa agattttcag ctttttttga gaaacaaatt caaattaatg
    14581 gtcgtaagtt tatttcttta accggtgtaa ttgaacttct aaagaatctt atcccaagta
    14641 tgctctatga tgtaaatgaa ttttgtatta tacatggaga tttgtgtttt gctaatatga
    14701 tggtagattc caattgttcc tttgttaagg ttattgatcc taggggaaaa tttggaaaat
    14761 atgatattta tggggacagc agatatgaat tagcaaaact atttcatagt ttagatggta
    14821 aatatgatta tatcatcaag gatctattta ctgtagaata tgataccgag tgtgcagtga
    14881 tagattatac aattaatacg cgtgtaagag attacgattt atttgaattg tttaagtcgg
    14941 tcttttcgga cgagattaag gacgattttg ataagattgt attgatagaa gcgttgctat
    15001 ttcttagtat gattccatta cacggtgaaa gttttgatca ccaaatagtg atgcttgcta
    15061 caggtatgga acttcttgca agagtaagta ataaagttat actctaatat aaattgtgcc
    15121 ttaatgaata acggttacaa tactgtttta gcaatgtagg cattaaggca cgattatatt
    15181 aattaataca atagaaggga agagaaaaat gtatagtgaa tattcttttg tgtttgatat
    15241 cgatggcact ttgtgtccga taaagaagaa ggatgagcga tatgaggatt tggtgccgta
    15301 tgagaatatg gttgaaaaac tgagattcta taaggagaat ggtgctaaaa ttacattgtt
    15361 cacctcacgc aatgttaatt cctataaagg aaatttggga atgataaatg ctaatactgc
    15421 aaaagtgctc ttggcgtggt tggataaatg ggacattccg tatgatgaaa tcctgtatgg
    15481 taagccttgg ccggggcata aaggcttcta tgtagatgat cgaacggtta gacctgatga
    15541 gtttttgaga tgctctgtag atgaattaaa tgaaatctgc aacagttcaa aaaaaagatt
    15601 ctaaataagg aggttagttc tatggaaaaa aagatgacag ttatcattac gatggcaggt
    15661 ttgggaagtc gttttcgaaa aattggttat gcggttccta aatatatgat taatgccaaa
    15721 ggaagaactt tatttgattg ggccatggat agtttggttg gatacaataa aaatgttgat
    15781 aagtatattt ttgttggtcg tcaggaagac aacgcttcag cttttataaa ggagcattgt
    15841 gcttactttg gtattgaaaa cgtagaagtg attgaaatag actatatgac agatggtcaa
    15901 gcaactacat gtatgttggc tattccttat tgcaatcaag acaatgagat tatggtttac
    15961 aatattgata catacgtgga atcaaatgag atgaaatacg aagatatttg tggagatgga
    16021 catattccat gctttcatgc tgatggcgat cattggagtt ttgcaaagct taacgatcga
    16081 aatgaagtaa tagaagtccg cgaaaaagtt cgcatttctg ataattgtac tcttggtgcg
    16141 tattatttct catcggcaaa gctgtataaa gctttatatg aagagtatta tgcagactcc
    16201 tctaatatgg agaagaatga aaaatatata gcaccattat ataactttat gataaaaaaa
    16261 gggttaaagg tgactattag catagtagat gcgaaaaagg ttcatgtgct tggtactcca
    16321 gaagagctca atgtgttttt gaatcaagaa taaatagtgt ttaggagaaa atagcagtga
    16381 ccagtaataa tctaattgta aaaacattaa gcagatataa gaatactcca cttcctgtta
    16441 aggctgcatt atggtttatg gtatgtggtt ttttacagag aggggtttca atgattacaa
    16501 ccccaatttt tactagagtt ttgtctacag aacaatacgg tttatacagc acttatctat
    16561 cgtgggaatc cttattgctc atgattacat cgcttagttt gttcaaagcc atgatgaatc
    16621 tttatgtaaa atatgataat caagaacata ttctttcgac agtatccgga ttgactttag
    16681 tactgactat tttttggttt gttattgcta tttttattaa ggatatattg gcagaaattt
    16741 taggattgac accagttcta gttgtctgta tgttctcata ttttatattt caggcagaaa
    16801 tacaatgttg gtctttacac aaacgatata tatatgatta taagacttta ttagtagcta
    16861 cccttgcagt tactgttggc tcatctgtag ttggctgttt agcggttctg tttatatctc
    16921 ctaccgctga ggcgagagcc ttaatgaatg tcctagtgac tggtgttgtt ggcgtggctg
    16981 tgtatatatc ggtatttaga agatgtaagg attttaagga taatgaagtg tggaatttcg
    17041 cagtagtatt ttgtgtatcg ttgatgccac actatttatc tgaatttatt ctgaatggtt
    17101 ctgataaaat tatgataaat tacttatgtg gttcgtcaga tgttgctatt tacagcgtgg
    17161 catattctat tggtgctcta attaaccttg ttacaaattc tgtcaattcc acttttgcac
    17221 cttatcagta tcaaaaaatc aagtcaggag aatatgattt actatcaaaa agagctaatg
    17281 aagttcttgg tttggtagcg gctatgcttt ttgcattaat gctatttagt aaagaaattg
    17341 tgttgatatt tggtgggcag aaatatatcg acagtgttaa tttaattgta cctatatgta
    17401 tcggtattta tttcaactat atgtttcagc tgtttgctag agtacaggaa tactttgaaa
    17461 agaaactcac tgtcgttatt ccatcggttt tgtgtgcaat tatgaatatt gtactaaatt
    17521 atgtttttat aaagttatat gggtatcagg cggtagcata cacaactgta ttctcatata
    17581 tgtttttctg cgtcatccat tatatttttt atagaaaagt ttgcaaagaa ctattggatg
    17641 gaaaaaacat atatgatgtc agaggaatac ttttgatttc tgcaatcacg atagctggtg
    17701 gcatcctaat ttcaataatt aataaatttg atttcataaa atatttaatt atagcaattg
    17761 cttgtgtcgc tgcaattata tttagaaaac gattgctact tttagtaagg agaattatga
    17821 gcaaataaac atttgctcat agagagatag agaatgatag atattatttc tgctataatg
    17881 gtaaatgtat taacagcatt atatcagccg ttcttatttg ccgtattaac agcgtatctg
    17941 tttatgtact ggtatctatt tatagttgat aactctcgag atcctaaaaa cttaaaagat
    18001 tctgtattaa tctggttgag atattttaaa gcatctagta aatttcgcag actctttttt
    18061 cttgcgtttt attttacttt gatattattt aaaaccttgc ttaatcgaaa tatgtggatg
    18121 aacccgcttt caaacgttat tggacagtgg tcattatata ttatagataa tgctacagga
    18181 gagagcagat tgacaacaga atgcgtggag aacacgatgc tgtttatccc tttttgcatg
    18241 ttattgttat ggtggcgtga cattaaatgt gatattatac gcaccatcta tgttgggatt
    18301 aagtatgtgt ttttattctc attaagtata gaatttacac aactgttttt taggcttggt
    18361 acatttcaac tatcggattt gttttataac accctagggg gcttaattgg tgctttgcta
    18421 tattggctat tctaccgatt aaataagtat atagacttaa aatagaactg ctttgaattt
    18481 taatatggtt aatatttttc tgcagacagg attgtgtaat ctcatcaaga atactttaca
    18541 tgccatctat ttttgctaaa acattgttgt tatatcttca agaggtaggg aattttattg
    18601 ctttaaatga ggtcgagcga attaaaaaat tggtaaaata tataacatgg tggtagttat
    18661 ggtaaaagtg tttgatataa ttaagttttc ggatttcaga ggatatactg ctgttgcatt
    18721 ggataatgag actcttttct cgattggtat tgagtttcac attaagcaaa taaatcaagg
    18781 atttagtcat aataaatata ctatgcgagg gctacattat cagagtggag agcatgctca
    18841 aggaaagata gtgatgtgcc tacatggtga aattatgaat atcgcacttg atattcgaca
    18901 aggatcttcc acttatggat gtgtgtatgt caacacattg agtgaaagca atggtaaatg
    18961 tgcgtatatc ccacgtggat atgctcatgg ctatcttact atggaggaca atacgctagt
    19021 tcagtggtgc gtagataatg atttcaattc taatgctgcc aaatgtataa gttatgaaag
    19081 cgtagatatt gaatggccgg tcagtcggca gtgtttcgtt ataagtgaga aagacaaaaa
    19141 tggggaaaga ttaagctttt gattaaaatg agctgtggag aaaagaagga tactggaaaa
    19201 taagaagaac aataaccgag caaatgctcg gttatttcta tgaggaatac ataaaactaa
    19261 atttagatat agatatttat tcacgaaaaa ctaatatt
//
LOCUS       DACEKH010000053        18978 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEKH010000053 DACEKH010000000
VERSION     DACEKH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.054"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(93..968)
                     /locus_tag="IW662_001693"
     CDS             complement(93..968)
                     /locus_tag="IW662_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0866518.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(1050..1841)
                     /gene="codY"
                     /locus_tag="IW662_001694"
     CDS             complement(1050..1841)
                     /gene="codY"
                     /locus_tag="IW662_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0866519.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(1859..3259)
                     /gene="hslU"
                     /locus_tag="IW662_001695"
     CDS             complement(1859..3259)
                     /gene="hslU"
                     /locus_tag="IW662_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0866520.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(3273..3821)
                     /gene="hslV"
                     /locus_tag="IW662_001696"
     CDS             complement(3273..3821)
                     /gene="hslV"
                     /locus_tag="IW662_001696"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0866521.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(3858..4763)
                     /gene="xerC"
                     /locus_tag="IW662_001697"
     CDS             complement(3858..4763)
                     /gene="xerC"
                     /locus_tag="IW662_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0866522.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(4961..6337)
                     /gene="trmFO"
                     /locus_tag="IW662_001698"
     CDS             complement(4961..6337)
                     /gene="trmFO"
                     /locus_tag="IW662_001698"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0866523.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(6306..8384)
                     /gene="topA"
                     /locus_tag="IW662_001699"
     CDS             complement(6306..8384)
                     /gene="topA"
                     /locus_tag="IW662_001699"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0866524.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(8506..9363)
                     /gene="dprA"
                     /locus_tag="IW662_001700"
     CDS             complement(8506..9363)
                     /gene="dprA"
                     /locus_tag="IW662_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0866525.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(9419..10186)
                     /locus_tag="IW662_001701"
     CDS             complement(9419..10186)
                     /locus_tag="IW662_001701"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0866526.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(10179..11039)
                     /gene="ylqF"
                     /locus_tag="IW662_001702"
     CDS             complement(10179..11039)
                     /gene="ylqF"
                     /locus_tag="IW662_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0866527.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            11299..11697
                     /locus_tag="IW662_001703"
     CDS             11299..11697
                     /locus_tag="IW662_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0866528.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            11934..12476
                     /locus_tag="IW662_001704"
     CDS             11934..12476
                     /locus_tag="IW662_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0866529.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(12559..13110)
                     /gene="lepB"
                     /locus_tag="IW662_001705"
     CDS             complement(12559..13110)
                     /gene="lepB"
                     /locus_tag="IW662_001705"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0866530.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(13262..13489)
                     /locus_tag="IW662_001706"
     CDS             complement(13262..13489)
                     /locus_tag="IW662_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0866531.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(13486..14004)
                     /gene="msrA"
                     /locus_tag="IW662_001707"
     CDS             complement(13486..14004)
                     /gene="msrA"
                     /locus_tag="IW662_001707"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0866532.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(14024..14737)
                     /locus_tag="IW662_001708"
     CDS             complement(14024..14737)
                     /locus_tag="IW662_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0866533.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(14740..15636)
                     /locus_tag="IW662_001709"
     CDS             complement(14740..15636)
                     /locus_tag="IW662_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0866534.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(15794..16636)
                     /locus_tag="IW662_001710"
     CDS             complement(15794..16636)
                     /locus_tag="IW662_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0866535.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            16768..17421
                     /locus_tag="IW662_001711"
     CDS             16768..17421
                     /locus_tag="IW662_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0866536.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(17484..18011)
                     /locus_tag="IW662_001712"
     CDS             complement(17484..18011)
                     /locus_tag="IW662_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0866537.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(18031..18978)
                     /locus_tag="IW662_001713"
     CDS             complement(18031..18978)
                     /locus_tag="IW662_001713"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0866538.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
BASE COUNT         5043 a         3971 c         3333 g         6631 t
ORIGIN      
        1 aatcgatgtc acactccctt attacttaca ggatagcttt tttacacacc ttggtaaacg
       61 gtgttccctg ctaacttctc ggtcacaacc tcttacttca ctgtgatgga atattttgtc
      121 ttgaagattt catgggcacc tagttgattg atccctactt tttccatcaa gtttccacta
      181 ctgttcaccg tgtcagcgat tccgcaccac ggttcgatac aaacgaaagg cgcttctttt
      241 ggataaggag accagatccc tacataaggc atatctttat agcttaaagt tacgctatgg
      301 gaagattttt cacttcgaat ggcgtacgca tttagacccc ttgtttcaaa aaccaaggca
      361 tcttgctcaa aaagttcacg attcaaagcc agattcgtat ttgtctgacc taacgtcttt
      421 tgatccatat cgatgaatgg gcctttcaat ggtaggcgta atcttgattt caatggagaa
      481 aatgccagat agaaatcttc aaatgacaat ccttcttcaa gaggtacatt gaaagcagga
      541 tgtccaccga tagaaaagaa catttctttt tcaccggtat ttgccacttg ataatgaacc
      601 gtgatgccgt ctcctcctaa ttcatagctg atcactaatt caaaatcaaa tggatacttt
      661 tctaatgtct ccggcgtact ccttaagatg aaactagcca tttcttgtcc atgttcaatg
      721 acttcaaaat ccatatcccg agcaaatccg tgttgcccca tttgatatgt tttcccttga
      781 taaacatact gatcatcttt taaacgtcca acaaaaggga ataaaaccgg tgcatgtctg
      841 ccccaatacg ctggatcacc ttgccagata tactctaaac catgttcttt tgatttcaga
      901 ctgatcaatt ccgcaccgtg ttcagcgatt tcggcgatca gctggtcatt ttgaataata
      961 acagtcattg ccattcctcc aacttcgact tactttttaa aatgggaagg ctggaacaaa
     1021 catttctatc tgccccagtc cccttacttc taataactgt gttttgctaa ctcgtcttta
     1081 aaacgggaat tcaatacttt taaataagtt cccttcatgc ctaacgaacg agattcaatg
     1141 attccagctg attccaattt tcgtaaagca ttgacgatca cagaacgagt gatgccgata
     1201 ctatcggcaa tattagaagc agtcagacga ccttcatctc catccaatgc ttcaaagata
     1261 gcttgtacag ccttcaattc gctataagat aatgtgttga ttgccatttg gacagccgtt
     1321 gcactccgta catctgcttc gatgcttcga gacttctgat aaaggatctg cataccgaca
     1381 accgtagctc catattctgc taagactaga tcgtcatctg caaacgattg ttccatacga
     1441 gataagataa tcgtacccaa acgttcgcca gctccaaaga tcggaacgac agtcgtcaga
     1501 ccaaatggat atttctctct tgactctacc ggaaatgcag taatatcact agtaattggg
     1561 atattagctt cagttttcat caactgtgcc actgtatcag tataagattc aggaaattgt
     1621 ttctcaacaa acattgtttt gattcgttca ttgttcacat cgtgacgttc attataacct
     1681 agtaatactc cgtcacttcc tatgatatag gtgttgctgt caagaatacc acctagtgtg
     1741 acagccattt tatcgtaagg caaatctgct tgcatgtcaa aagtattctt ttgctgcaaa
     1801 agtgtgttta tcttgcgggt tttcgttagc aagtctgtca ttgtcgtcct ccgatttttt
     1861 ataaaatata gcgacttaaa tcttcatttg ccgcaattga gccaagtttg tcatccacat
     1921 atgcttctgt gatcgttatt tctcccattt gcatatctgg tgcttcaaat aatagatctt
     1981 ctagtagttt ttccaaaatg gtatgcagtc tgcgtgcgcc gatattatct gtgtcacggt
     2041 tgacatcgaa agcgatttga gcgatccgtt caattgcttc ttttgtaaac gtaactgaca
     2101 cattttctgt accgatcaac gcgacgtatt gttttactaa agcattattt ggttcggtca
     2161 gaatcttcac aaaatcttca gctgtcaaat catctaattc tacccgtatc gggaagcgtc
     2221 cttgaagttc tggaattaga tcacttggtt tagacaaatg gaaagctcct gaagcaatga
     2281 ataggacgtg atctgtttgg atgatcccgt attttgtgtt tacttgtgaa ccttcgacaa
     2341 ttggcaatat gtctctttgg acgccttctc tggatacttc tccagattgc tgtgatttag
     2401 aagtgatctt atcgatttca tcgataaaaa taattccact ttcttccgct aatttgattg
     2461 cctcactatg gatatcagca tccttgacta attttgctga ttcttccttg atcaataact
     2521 ctaaagcttc tttgactgtt accgtccgtt ccactttttt cttcggtgtc aaagcaccta
     2581 gagtttcatt taggtcgatc cccatttgct ccaatccatt gttcatcatt ggtgcaggtt
     2641 tcttttggtc ttcaacttca atggttactt cacgactatc cagtaaaccc ttttgtagtt
     2701 gttccaagac tgtacggcga ttgattcgaa tatcttctgt cacttcttct tgcggttcag
     2761 cggtattttg ctgactagca ttaaaaagat tcatcatttg ttcaaattga ttagtggatt
     2821 gtttctgttc ttttttgata ccaggtacca agatttttac caaacgtttt tctgcatttt
     2881 ttttagcact tgtgtataca cggctgtact gctctttttc tacgatttgg atcgcatttt
     2941 ctactaaatc ccgaaccatt gattctacat ctcttccgac ataaccgact tcagtaaatt
     3001 ttgttgcttc gactttgaca aatggtgcat tgacgatttt tgccaaacgt cgtgcgattt
     3061 ctgttttacc gacccctgta ggtccgatca tcagcatatt ttttggtgta atatcttgtt
     3121 gcattttttc atctaactgc aaacggcgat agcggttacg caaagcaacg gcaacagatt
     3181 tttttgctgc gtgctgtcct acgatatatt catctaattc acttacgatt tgtcttggtg
     3241 tttgttttaa ttcatacata tctactcacc tcttacagtt cttctacgat aatattatga
     3301 ttagtaaaga tacagatatc tgcggcaata tttaacgcat tttctgcaat ttctttcgca
     3361 ctcatgtcgt tttgagcata gtttttcatt gctcgggctg cagccaacgc aaaattccct
     3421 cctgatccga tagctaagat accatcatct ggtgtgataa cttctcctgt acctgaaaca
     3481 agaagcattt ctttatcatt cattacgatc agcatcgctt caagtttttg catagattgc
     3541 tgtgtccgcc attcttgagc taattcgaca gctgcgcgtt gtagattccc gttatattcg
     3601 tttagtttgc cttcaaattt ttcttctaaa gtaaacgcat ctgcaacact tccggcgaaa
     3661 cctacaacaa cttctccatt gtagatacgt cgtactttct ttgcagtgcc tttcatgaca
     3721 acagattcac ccattgttac ttggccatct ccagccatag caaatttccc gtctttttcg
     3781 acggcacaga tagtggttga atgaaatggt gattcgacca tgtttgtttc ctcctaaatt
     3841 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
     3901 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
     3961 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
     4021 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
     4081 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
     4141 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
     4201 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
     4261 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
     4321 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
     4381 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
     4441 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
     4501 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
     4561 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
     4621 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
     4681 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
     4741 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
     4801 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     4861 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     4921 agagcgagac tatccgtctc accctcttca tggagtttcc ttactcattt gggattgcag
     4981 ttttttctaa ttctgttttt attgcctcat tagcttttaa agctcgttgt gcaattgctt
     5041 catatcttgc ttttttgtct cgaatgcgct ctggcagttc aggaaacaga ccatagttgg
     5101 cattcatcgg ttggaaatgt tttccttccg catgagtaat gtagtaagcc atacttccaa
     5161 tagctgtttc ttgtggaaac tcgactggtt ccatttcttt tgctaagcgt gcggcgttga
     5221 ttccagcaag tagtccgctc gctgcacttt caacatagcc ttctacacca gtcatctgac
     5281 ctgcaaagaa cagatcttcg cgtttcgttg attggtaagt cggtttcagc aattctggcg
     5341 aattcataaa gctgtttcgg tgcatcacac catagcgaac gaattcagca ttttctaacc
     5401 caggaatcat ccggaaaaca cgtttttgct cgccccattt caagtgagtc tgaaagccca
     5461 cgatgttgta taaagaagct gcagcattgt cttgacgcag ctggataaca gcataaggac
     5521 gtttgccagt tttaggatct tccaatccaa ctggtttcat aggtccgaaa agcatcgttt
     5581 tgaatcctcg ctgtgccatt acttcaattg gcatacatcc ttcaaaatat ttttctttct
     5641 caaaagattt aagttctgcc acttcggcat tgattagtgc ctcatggaaa gctttgaatt
     5701 cctcttctgt catcggacag ttcaagtaag ctgcttctcc tttgtcatag cgggacttca
     5761 aatatacttt atccatatcg atcgtagctt tgtctacgat tggtgcagct gcatcataga
     5821 aataaaaacc atctgaacca ttgaatgctt tgatgctttc tgctaatgga tgagaagtca
     5881 aaggtcctgt tgctactacg gtgattcctt ccggtatttc agtaatttct tcgtttatca
     5941 ctgttactaa tggatgctct ttgacttttc gggtaattgt ttcagaaaaa gaatcgcggt
     6001 caacagctaa ggcaccacca gctggcactg ctgtttcatc tgccgaagtg attactaccg
     6061 aattcaatcg gcgcatttct tctttcaata accctactgc attagccaaa ctgtttcctc
     6121 ttaatgaatt ggaacatact agttcagcaa aattacttgt gtgatgcgcc tcagtcgatt
     6181 tgactggacg catttcatat aaacggacag ggactcctgc ttgtgcgatc tgccaagcag
     6241 cttcacttcc ggcaagccct gctccaacaa catttaccat tttcaatgaa agaacctctt
     6301 ttctattatt tttggatgtc ttcttcgtaa tctccattta cacagactac ttgtttaccg
     6361 cctttgactt tcttctcgac cagatatccg tcacattttg gacaatttct gcccactggt
     6421 ttatcccaag aagtgaattc acattctggg aaacgggaac aaccatagaa tagtctgttt
     6481 ttcttagatt ttcgttcaat gacttgtcct tcatgacata ctgggcaagt cacaccgatt
     6541 tcttttacga ttggtttcgt attccgacaa tcaggaaaat tactgcaagc ataaaacttc
     6601 ccatatttcc ctaattttat caccatcgga tgtccacaga catcacaatc gaatcctgct
     6661 ggttcatctt tgatttgaat tttttcgatt ttttcttccg cattcgtcag ttctttttca
     6721 aacggtttat agaaacgatc aaccactttg acccacgctt ccgtgccttc ttcaatttta
     6781 tccaaatctc cttccatttc tgctgtgaaa tggatatcga cgatttgagg gaagaattca
     6841 cagattaatg aattgacaat ttctcctagt tcagtcggct caaatctttt ttgtgtcaat
     6901 tttacatagt agcgtctttg gatcgtatca agtgttggtg cataagtgga aggtcgccct
     6961 accccgtttt cttctagtgc acgaatcaac gtagcctcac taaaacgtgc tggtggttga
     7021 gtaaaatgtt gttttggttc aatatcaaca gattggacta catcgccttc ttcaagttct
     7081 ggaagaatat tttctttctc ttctttgccg tcatctctac cttccacata cacttgcatg
     7141 aatcctttga atttgatttt tgaaccgttc gcaatgaaga tgacaccatt ttgttctaat
     7201 gtcactttca ttgtatctaa aatagctggt gtcatctgac tagccacaaa acgtgaccag
     7261 atcaatgtat aaagtttcaa ttgatcttta tctaaatatt gtttgagttc atctggtgta
     7321 cgaagcacac tcgttggacg cacagcttca tgggcatctt gtgcgccttg agcatttttg
     7381 atctttcggt gatcatgtct agaaaattct tttccatacg tatcttcaat aaatgctgcg
     7441 gcctctgctt ttgctgtatc ggcaatccgg gtagaatcag tacgcatgta tgtaatcaag
     7501 ccgactgttc cttgtttacc caaagtgatt ccttcatata attgctgtgc taccatcatg
     7561 gtttttcttg ttcgaaagtt taacttacgg gcagcttctt gttgcaggct acttgtagtg
     7621 aaaggattcg cagggtttct tttgcgctct ttcttttcta cttttttaac ttcgaagtct
     7681 gttccatcaa ttctagcagt gacttctttt acactttgtg catctggtaa tttctttttc
     7741 ttaccgtcga ttccccagaa atttgctttg aatttcttgc gagctttttt aaaattaccg
     7801 tcgatgctcc aatattcttc tggaacaaat tctcggatct ctttttcacg gtcaataata
     7861 atttttaaag cgacagattg gacacgtcct gcacttaacc ctttcttgac cttacgccag
     7921 agaataggac tgatcgaata accaacgatt ctgtctaagg tacgacgagc ttgctgtgca
     7981 tctactaaat ctacatcgat cgttcttggt tccttaaaag ctgctttgac tgcgtctttt
     8041 gtgatttcat tgaagaccac ccgatttttt tctttcagat ccaacccaag taggtacgct
     8101 aaatgccaag caatcgcttc tccttctcta tccgggtcac ttgccaagta aactttttga
     8161 gctttttttg ctgcggcttt caggcttttg atgacatcgc cttttccgcg tatagaaata
     8221 taatggggtt cataattgtt ttcaaaatcg atacccattt tacttttcgg taaatctctt
     8281 atatggccaa cactggccat tactttatag tttcgtccaa gatatttctc aattgtcttg
     8341 gcttttgcag gtgattctac aatcactaaa tatttatacg ccattactga atggactcct
     8401 ttccattgtc gtgttcctac tattaaaaga ggattcatac aaatcgtagc taatcatatc
     8461 cattcagaaa acgtttgtca agaaaagcaa atgaaaagta tggtgttatt ctttaaattc
     8521 tgccaagata tcttcacccg aaaccgtgca aactgcaccg tcttggatca aatgatgaca
     8581 tccggtagaa tgttctgtta aaatatttcc tggaacagaa aaaactgttc ttccaagatc
     8641 caatgcttgc tgtgctgtta tcagagagcc gctttttttc ttggcttcaa agacacatgt
     8701 tcctttagac aaagctgcga taatccgatt tctcattgga aaacaatgct ttttcgggcc
     8761 ggattgagga ggatattcac tcaaaagaag atgatcattt gcaatcacct cttgcaagtc
     8821 acgattttca gcaggataga cttgctctat ccctgttcca atgactgcga ttgtccgtcc
     8881 accatgagag attgctgctt ggtgaccgta tgtatctatc cctttcgcgc agccactgac
     8941 gatcacatat ccagcttcta tcactggttt tagtaaataa taaactgttt ttttggctaa
     9001 agcagaagtg tgtcttccac caacaacgct taacatgtta caagaaagta aagatagatc
     9061 accattataa aacagtacaa ttggcggata ggtcaagtga agcagctgag ggggatattt
     9121 gggatcaaag caagtgataa acgactgttt cgattggatt ttgccccagt tagcgtgaat
     9181 ttcctgccaa cttttgcgaa acagctcccg gtgtttggtt attttaccta tcttaatgat
     9241 gtcttcaaat ccgatttctt tataaggata ctgcatcatc cagttagcaa gtgcccattt
     9301 tccttgacta ctgattcctt ttgtataagc aagtttcaat aaattttctt ctatttgata
     9361 catcataaaa ccgcctttcg tctctctttc taataagata cgtaaaagcg gctgtttttt
     9421 attgtaaata tttttttaca ggtgcaaaac ttttacgatg gattggcgta attccttgtc
     9481 gttcaagtcc ttctaagtgt tcctttgttc cataacctgc attatgcgag aaaccatatc
     9541 caggataaag cacgtcatat tctttcatca attggtctcg atatacttta gcaatgatac
     9601 ttgcagcagc aatcgacaca gatcgtgcat cccctttgat tattttttcc tgctgaatag
     9661 gcaaatccaa ggtcatcgca tcgatcaaca aatagcttgg tacaatggac aatttttcta
     9721 ctgcttggat catcgctaat ttacttgctt cataaatatt cacatcatcg attgtctgct
     9781 gatcaacgat cccgatcccg attgccaggg ctttttcttg aacttgttga taaagaagtt
     9841 ctcgcttttt ctctgataat tgtttggagt cattcagccc taatatgatc tcatttttcg
     9901 gcaagataac tgcagctgcc acgacaggtc cagctaatgg tcctcttcct acttcatcaa
     9961 tgccggcaat ccattggtga ccttgtgcgt acccttgctt ttcaaagcaa aacatttctt
    10021 catatttttc cttaattaat gcttcctttt tcaatcgtcg ttcaaaagaa gccagtgctt
    10081 gctgcacgcc tttgcgtgga tcatttctta atgattctag tgtaggatca tctattctct
    10141 gtatgctttt caatcgttct ttgatacttt ggatggattc agtcatcact tttttgctcc
    10201 atttcttcgc aacgatccag tgtgtatcgt ccaagttttc cttgacgaat atcatgcacg
    10261 actaattcgc tgccacgatc atagtcttct ttatacccac gttttgctgt aatatccatc
    10321 agtaattccg gtgccaattt ttcttcttgc tcgattgata aaccataacg atcgctgatt
    10381 cttcctgaat aatatcgggc aaaaaagtct aatccgtaaa tcgctaaatc atctaaatgg
    10441 acaagctggt ctttgatcgc gcctgtcagc gctagttttt tgccgatttc ttcatcttca
    10501 aatttgggcc ataaaatccc tggtgtgtct aacaattcca actcattacc taaacgaagc
    10561 cactgctgac cttttgttac gccaggctta tttcctgtct gtgcaatttt cttgcctact
    10621 agtcgattca tcaacgtaga tttcccaaca tttggaatac ctaagaccat tgcccgaata
    10681 gcgcgaggct tgatcccccg agcagcatct cttgcccgtt tttctttcaa tgcttcttta
    10741 gccgcaggta ctagaccttt aattccttta ctttcttgtg ccgtgatagc taatgcaaga
    10801 tggccttgtt tacgaaaata ttcttgccac agctttgtct gttgtggatc tgccagatcc
    10861 gctttattca ataaaacaag gcgaggtttt tgctgaatga tctgatctag cattggatta
    10921 cgagaagata aaggtaatcg tgcgtctacc aattcaaata cgatgtccac atatttcatt
    10981 ttttcagaaa cttctcgtcg ggcttttgcc atatgtcccg gaaaccattg gatggtcatt
    11041 gttctctcca ttcctttcat accggaatat tccccggctt tacttgtttt tttctttttc
    11101 aatataaatt cactttttta ttataaagaa attccatcta ttagttaagt gaaagtgaac
    11161 cagtcctcat tctatcacat cttcactttt ttgatgatgc aaatttttat tttggtttct
    11221 atatatgaat ctaaattttt gacagcgttt cctaaataag agatgataga cgaacaaaac
    11281 agaaaggaga cgaaacaaat gaatagtatg gtttttgtta attttcctgt tgctgatgtc
    11341 aatcgttccg ttgcttttta tgaaaagctg ggttttaaaa aaaatgaaga attttctact
    11401 gaagaagctg gttcaatggt atgggatgac actttttgga taatgcttct ctctcatgat
    11461 ttttataaaa aatttataaa agataaagaa atagctgata ataaaaaaac aagtggtgct
    11521 ttgatttcgt ttagtgttga aaatgcggat gaagtaaaaa aatttgctca aattgcaaaa
    11581 gaaaatggtg gtactttcca cgaagtagat atgggaatgc ctgaagaaga aatgttttca
    11641 ttagaagtcc aagatctaga cggaaataca ttagaacccg tatggatgaa gatgtaagca
    11701 gataactaat ctgacgtttt ttataacaaa aaaacaaagg cggatgatac gataagtatt
    11761 ctccgcctct atttttttta ttcgtacaat ctctttttta cagtttataa aagcagtctt
    11821 ctcacccttt ttttaattgt tagatgccag tgtatgtaaa tcaccaatgt gtcatgacac
    11881 attggtgatt ttatggtatc cttttattat taactgacaa aggagagaat attttgaaca
    11941 ctttgattgc aaaagacgtt tttagattgc ccgaaatgaa tgcaagattg ttgtcgacct
    12001 ctgctctttt attagccctt gatgtcatct tatacaagat agcgattggt cctgcttatt
    12061 ttcaaatcac attcggattt ttaagtatgg cgcttatggg ttatctatat ggaccgatct
    12121 gggctggttt gcttgaaatg ttgtcgaaca ctttaaattt cacgatattt ggttcaggaa
    12181 cgtttcaagc tgcttttctt atcacggctt tccttggtgg atttattaat ggcttatttt
    12241 tatatcataa aaagataaac ctgccttccg tcatcgctgc ccaattattg attatgatcc
    12301 cagtcagcct ttttatcaat tcttggctga tgtctgtttt gtttggtgca gatttcaaag
    12361 taatttttct aggtagattc atcaggaata tgatccaaat ccctattcaa ataatggttg
    12421 tttattcatt tatcaaaact ttagaggcta gaaaagtgtt taaacgagct gtttgatttt
    12481 acgtactaag gtagtaaact gattttggat taaacaataa agcgtgcagt ctcaacagac
    12541 tgcacgcttt attgtttctc actggatcca ttttatttca tcaagtggat aatagacaaa
    12601 tcttgcttta cctagaatat cagttttatt gatcgtgcca aacgaacgac tatcttttga
    12661 aactcttcga ttatctccaa gtacaaaata attatcttcc ggaagcttgg attgtcccgt
    12721 tagctcttgt agtgtaaagt ccgtcgtata ggatgcattt gcatgatctg atttcaggtt
    12781 tttagttaga taaggttcct taactacttt cccattgatc ttcaattgat cattttggta
    12841 gctgacattt tctcctggaa gaccgattac tcgtttgata taaatcgtgc catctgctaa
    12901 ttgaaaaaca acaatatcaa atcgtctgat ctccgaaaat ttttccatca caaccatatc
    12961 accttgcttc aacacgtttt ccattgaatt gccttccact ggtaccggga tgaataaaaa
    13021 tccccgcaac atgaacgcaa ggaatacaga aacaagtaag tatttgaata caagccagaa
    13081 gcgattaata tagcgttgtt tttttgtcaa tctctatcaa tccatccctc tttttttcat
    13141 tctttcttta ttataagaaa aatatcaaaa aaatgcgact agatagctga aataaaaata
    13201 attctttcaa ttaggctatt ggacaaaaaa cttttttgat cataaagtac aaaactatct
    13261 cttatatgtc atgattattt tctaaatatt tttcccatgc ttcgtcaaag atatccatgg
    13321 acggcaaata gtcgacattc agttccagat aattagaaat ttcttcgtag ctagagctgt
    13381 gcttaggaaa ttggatgtct ttaccggctt ctgtagcgaa aacagataaa gcatcatgtg
    13441 tgccaccgcc tcgcagtgtc attaaataat gaaaaaagct tcgattcacc cttcgttcct
    13501 ccaattttct tcgataaagg cagcccggcg tgcatggttt cttttaaaat tttcaggatg
    13561 ctttttataa tactcttgat ggtcttcttc tgctagataa aatggttggg ctggttcgat
    13621 ttttgtaacg attggctgtt cgaaacgtcc actttcagct aactgtttct tacttttttc
    13681 agcaatgatc cgctgttctt ctgtcaaata aaagatcacc gggcgataat tgtcaccgcg
    13741 atcctcgaat tgtccgaaag cgtcagttgg atcagtttgc tgccagtaaa gttctactaa
    13801 ttgttcatat gaaatcaatg ttggatcata gataatctcc actgcttctg tatgtccagt
    13861 cgtttggctt tttacttgtt cataagtcgg atctggtaca tgccctccag tatatcctga
    13921 catcactgta tggataccag gaagcgtgtc gaaaggctgg atcatacacc agaagcagcc
    13981 tcctgcaaat attgctcgtt ccattatgat tccccctcta atactactca tccgatgatt
    14041 ttttattttc ttttgtcttg ctttcagtac tgctggtgct agttttgttg gtatttgagt
    14101 tgtttgaagc atcagaggta tcttttggca aatatagact aaaccgaatg tcatcattta
    14161 ctaagtcgat tttatctgct ttaatataca tcccgttttg cattcggaat tgatccaacc
    14221 taagcatgat agtcagatca tctgtattga cttcgatcca atctggtaat ttatagtttc
    14281 tcttgatcat attcatcaca tctttgattg gcaaactcaa tgttccgata gaaagacttc
    14341 tagcttttag ttggacattt ccgttttcca tcacatacgg atcaaaatat aaatagaaat
    14401 cgataggaaa acctagtact tcgaaagtac cattgagtag tgcttcattt tccaaataaa
    14461 atttatattc aatatcacta tctttttgat actcactaag gaaaaagctg atgatttgat
    14521 tgactttttc tttatttgaa ttgatcgtta agacaggatc tccttgtctt tccgtaagag
    14581 ctggtatttc tgggagttct ggttcccgat tagcaaaaat acggctgcct aaaaatacgg
    14641 tcgaaccaag tactacccca agaaggataa ggaaggcgaa tttccagtaa tttgttttct
    14701 ttgttttgtt tgaagatacg tcagatcttg ttttcatgtt tattgatcat cctccttctc
    14761 taaccattgg ttttgtgtct cgatcagctg atctctcact gcatttgcca tcaattgata
    14821 acctaaatta tttggatgga aatgatcttc ttcatagagc acattatttt tgatgccatt
    14881 tagattttct gtcgtgctag ttgtttgatc tgttccttct tctgtgacac ctacttcctg
    14941 attcaatcct tgataaagca aatcattgat tgggataaag tagcagttcg gcattttttt
    15001 tgtcaccgtt tctgttcctt tgttccaatt gttaacaatt gtctgcatgt cagtgatctc
    15061 aggaaagttc aaataaaatg gattgtagat gcctaaaatg taaataggtg cggttttatt
    15121 gagggatcga aattcttcca atagttctgt gacattttcc tgatagtttt tcagtggctt
    15181 tttaaaggaa ttgaccgtta acccgaataa attattttgg ataactttca tcaaatcatt
    15241 accgccaaca gtcagtgtga taaaatcagc tgttttgata ttgtttcgaa tttcttcttt
    15301 tgtttttact cgctttaaga tctggtcact tcgttcacca tttacaccat agttttctgt
    15361 ttcaaccgcg gtcaaattat aacggtcttt tagatcattt gcgacaaggg gaacgaatcc
    15421 tccttgcttg gtttgatctc ctactccttc agtcagcgaa tctccgatag ctatgtagtg
    15481 aagtttttct tttttatttg gatcttctct cacagtagtt actggtttca ttagtgggcc
    15541 agctttcggt atagaagcag ctaaacctgc atagatgatg atcgcaatta ataatgggaa
    15601 caaaacgagg atcaaatgtc gtttcgttaa tttcattttt tcacctcttg tattcgattg
    15661 gttctaatgt tatcaaaaat tttatcaaaa aggtagtctt tatgaaaaaa gaaaacaatt
    15721 attttcctaa ttttataaag aaaaagagaa taagctgcaa gcctttcagg ctgagggctt
    15781 atcctctttt cgactaatct acataataca tgattgcaaa cgcgtcttta ccagtatgcg
    15841 tagcgataac cggattcgta tgcagtacag ggatatgcat gtccgggaat aattcttgca
    15901 aaccctcttt gaattcttgc gccaattctt ttgcatccgc atgtgaaata ccgatttgtc
    15961 gcacatttgg caattctttc agttcatttt tcaaattatt aaaccattta tcgaacgttt
    16021 tctttcctcg ccccttagtt acagggatta attcagaatg ggcgaaattc atcaccacgc
    16081 gtatgttcaa aacactagac agcaaaccag ttgttcggct gattcttcca ccttttacca
    16141 agttatctag tgtggatatt ccgatgtata attttgtatt gtcttgtact tttttgactt
    16201 cttcaaggat tttctctact gttttacctt cttttgctaa ttcagctgct cgaattacct
    16261 gaaaagataa tccttggtca gtggtatcac tgtcgataac cgttactttc gtttttgtta
    16321 gattactggc ttgtcttgct gcttctactg ttccactcaa gccttttgtc atatggatcg
    16381 aaatcacttc actgccgtct tctcctagct catcatacat cgagatgaat tctccaattg
    16441 gtggttggct tgttttgggc agattttttg ctttcgccat catttccata aatctttcgc
    16501 cgtctagttg gtcatcatcg ggatatactg ttccgtcgat catgatggat agcggcatga
    16561 cacgaatgcc taaagcatcg cgtacatttt tttccatcgt acatgaagaa tctgttacga
    16621 tttttacatt tgtcattatt ttctctctct ctttctttaa caagatgcct tattcatgtt
    16681 aaactacatg taagacaatt tctattttta gtataacaga caaataatga tttttcatta
    16741 attacaccta gtaaaggagc cgttttcatg gaaaaaacga aatttaatcg aacgtatgaa
    16801 atattaaatg aagtgtttaa tgcagtcacc catggaattg ggactggatt gagtatcgcg
    16861 ggactagtgg ttcttttagt aaaaggtgcc cacttgcatt cctccctcca tattgtctct
    16921 tatgctatct atggatcgat gatgattctt ttatttttaa cgtctacttt gtttcattca
    16981 ttgattttta caaaagcaaa aaaggtgttc caagtattcg atcatagttc gatttttctt
    17041 ttgattgcag gaagttatac tcctttttgt ttgctgagta tccgaggatg gctaggttgg
    17101 actttatttg tattaatctg gttgtttgcg attcttggca ttatatataa agcgttgacg
    17161 ttgcataaac gggagagagt atcaaaagta tctacgatca tctatatttt tatggggtgg
    17221 ctctgtgtcg tggctgctgt tcctctttat cactctcttg gaatgactgg tgttattttg
    17281 atggtagcag gcgggctttc ttacacgata ggcgcattct tttatagctt gaattctatc
    17341 cgctatatgc atgttgtttg gcatttgttt gttatgttag gtgcaggttt tatgtacttc
    17401 tctgttttat tctttactta ataaaaaaaa taaagctatg gatgggacag ctctaaataa
    17461 cagctagtct atccatagct tttttatatc gtcaattcgc ctgtcccgct ttttacagga
    17521 ttctcattac gctgatacgt ttcaaattga tgcggataac gatttttttc atccatttct
    17581 ccttgactag tattgatcag tgaccaatca gaccagtcaa caggtggaaa ataagcatct
    17641 ccatcgaata catgatggat gactgtacgg tataatatcg tacaaaatgg cagaaattct
    17701 tgataaacag ctgaccctcc agcgatgaaa aagataccgt ctgtttcttt ttccagagca
    17761 agcacgtctt ccacactatg catgacatgc acgccttctg ctttatagga agaatctctc
    17821 gtcaaaacaa tcgtctgacg attcggcaac ggacggctac ccatcccttc gaatgttttt
    17881 cgtcccatca ctagtgtatt tgcttctgtc atttgtttga agaatttcag atcatttggc
    17941 aaacgccaag gaagacggtc ttcttttcca atcaatccgt tttcatcctg tgcccaaatt
    18001 gcagctaaca ttttctcatc ctttctgatt ctaaacagcg ataggagctt tgatcgccgg
    18061 atgtgggtca tatccttcta attggatatc ttccatctca aaatcaaata ctgactgctt
    18121 ttcctgattc aaaactaaag tcggaaatgc gcgtatttct cggctcaatt gttctttcat
    18181 ttgttcgata tgattgatat aaagatgagc atctcctaat gtgtgaacaa attcgccaac
    18241 cgctaaacct gtttcatgtg caattaggtg agtcagcaac gcataacttg caatgttgaa
    18301 cggaacgcct agaaacacat ccccactgcg ttgatacaat tggcagctta gccgtccctc
    18361 ttgaacataa aattgaaaca tcgtatgaca aggaggcaaa gccatgctag gaacatcttc
    18421 cggattccat gcagaaacga tcaatcttct agaatcagga gtctttttga ttgcttcgat
    18481 cacttgtttg atttgatcga taaagccacc gtcttttgtt tcccagtgac gccattgata
    18541 gccgtagatg tgtccaagat cgccgtatgt ttcagcaaag gaatcatcgg ttaggatctt
    18601 ctcgcaaaaa agcttgctct gctttttata ttcttcattg aattcaggat ctgtcaaaca
    18661 tctatggcca aaatcagtca tatctggtcc attatagtct ggacttttga tgtagcgttc
    18721 aaatgcccat tcatcccaaa tatggttttt attttgtaat agatagcgga tattcgtatc
    18781 tccttttaaa aaccaaagca attcgctttt gatcaatcca aaaggtactc gtttcgttgt
    18841 taacaaagga aacccttttt gcagatcaaa ccgcatttgg tatccgaata agcttaatgt
    18901 gcctgtgcct gttctgtctt ctttacgatg cccttcttcc aacaattttc ttcctaagtc
    18961 aagatacgct tgttccat
//
LOCUS       DACEKH010000054        18776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEKH010000054 DACEKH010000000
VERSION     DACEKH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.055"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(17..2101)
                     /gene="cadA"
                     /locus_tag="IW662_001714"
     CDS             complement(17..2101)
                     /gene="cadA"
                     /locus_tag="IW662_001714"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0866539.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(2243..2431)
                     /locus_tag="IW662_001715"
                     /pseudo
     CDS             complement(2243..2431)
                     /locus_tag="IW662_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(2625..2984)
                     /locus_tag="IW662_001716"
     CDS             complement(2625..2984)
                     /locus_tag="IW662_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ0866540.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3014..3355)
                     /locus_tag="IW662_001717"
     CDS             complement(3014..3355)
                     /locus_tag="IW662_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866541.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(3352..4026)
                     /locus_tag="IW662_001718"
     CDS             complement(3352..4026)
                     /locus_tag="IW662_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0866542.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(4821..6119)
                     /gene="asnS"
                     /locus_tag="IW662_001719"
     CDS             complement(4821..6119)
                     /gene="asnS"
                     /locus_tag="IW662_001719"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ0866543.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6159..7349)
                     /locus_tag="IW662_001720"
     CDS             complement(6159..7349)
                     /locus_tag="IW662_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0866544.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7370..7897)
                     /locus_tag="IW662_001721"
     CDS             complement(7370..7897)
                     /locus_tag="IW662_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0866545.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(7944..10733)
                     /locus_tag="IW662_001722"
     CDS             complement(7944..10733)
                     /locus_tag="IW662_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0866546.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDSSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAIYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKEIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(10880..11080)
                     /locus_tag="IW662_001723"
     CDS             complement(10880..11080)
                     /locus_tag="IW662_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0866547.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(11532..11852)
                     /locus_tag="IW662_001724"
     CDS             complement(11532..11852)
                     /locus_tag="IW662_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866548.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(12153..13271)
                     /locus_tag="IW662_001725"
     CDS             complement(12153..13271)
                     /locus_tag="IW662_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866549.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            13742..15049
                     /locus_tag="IW662_001726"
     CDS             13742..15049
                     /locus_tag="IW662_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0866550.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFDNLF"
     gene            15168..16367
                     /locus_tag="IW662_001727"
     CDS             15168..16367
                     /locus_tag="IW662_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0866551.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(16394..17662)
                     /locus_tag="IW662_001728"
     CDS             complement(16394..17662)
                     /locus_tag="IW662_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ0866552.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLITLTL
                     FGTITTHLFGGSVGREGTAVQMGGSLADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(17825..18472)
                     /locus_tag="IW662_001729"
     CDS             complement(17825..18472)
                     /locus_tag="IW662_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ0866553.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILNHELPEIDTFLTTVLDVKLTKAQAERALEKLKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
BASE COUNT         5351 a         4005 c         3313 g         6107 t
ORIGIN      
        1 tcgtcctctt cattgatcaa cgtttatttt ttacatgcaa gcttcgcatg gcattcaaaa
       61 cagctaatac tgtcacccca acatctgcaa atactgcatc tcccattgat gcaaaaccaa
      121 aagcacctaa ggaaagtacc aaaattttta tcccaatagc aaagacaatg ttctgtttga
      181 cgattttcaa tgttttcttt gctaaatgga ttgcttctgc aatcttcgat ggttgatcat
      241 tcatgatgac tacatctgct gcttcaattg cagcatcgct tcccaatcca cccattgcga
      301 ttcctacatc tgctcttgct agcaccggtg catcgttgat tccatctccg acaaatgcgg
      361 tctttttatt gttactttgt aagatttcct ctaaccgctg cactttatct tctggaagca
      421 attcgctata tatcttgtcc acgcctactt gcttcccgat atgatcagca atttttttcg
      481 agtcgcccgt caacatgact gtgtttttca caccagcctg ttttaattga accaatgcgt
      541 ctacggcatc ttctttcaag gtatctgcaa taacaagata accactgtaa ctttgatcga
      601 tcgctagata aagcaatgta ccgatttctt gacttgaagg aaaagagatt tcaaatcttt
      661 ccatcaattt gtgattacct actaatactt gtttgccatc aacttcagct aagatccctt
      721 cacctgcaaa ttcctggata ttagctgttg gtaaaagcgg acctgcgtag ccttctaaaa
      781 cagattgagc aatcggatgt gtagaaaact gttctgcact tgcaacatat tgcataaatt
      841 tctttggatc aattcctgtt ggatcgatcg tctggatcga aaagtcacct ttcgttaatg
      901 tgccagtttt atcgaatacc actgtctcag tttgtgcaag caactccaag taatttgaac
      961 cttttactaa tacaccgatt ttacttgctc ctccgatacc gccaaagaaa ctaagtggca
     1021 cggaaatcac taatgcacat ggacatgaga tcactaaaaa tgtcaatgct cgatagatcc
     1081 attcagaaaa cgccgctcta gaaacaacaa gtggaggtaa aacagctaat aaaacggcca
     1141 atcctacaac gactggtgtg taatagcgcg cgaatttagt aatgaaattt tcagctggtg
     1201 cttttttact gctggcattt tctactaatt ctaataattt gctgatagta gaattttcat
     1261 aagatttttc tacttgaact aaaaatggtt tatttttatt gataaatccg cttaatactg
     1321 tttcaccaac atatacagat ttagggactg attctcctgt aagtgctgac gtatcgacta
     1381 atgattctcc ttcgatgatc gttccatcta acggtacacg ttcaccaggc ttcacaagta
     1441 caaactgacc gatcagcacg tcttcagggt tcacggtcat caatccttcc gccgtctgca
     1501 cgtgagctgt ttctggacgg atcgccatca attcgcgaat cgattttctt gaatgactga
     1561 ctgccaatcc ttggaaatac tcgccgattt gataaaatag catcactgct acggcttctg
     1621 gatattcacc gatcaacata gcgccaatcg tagcaacaga cataagaaaa ttctcatcaa
     1681 agatttttcc tttgacgata ttcatcactg ccttcttcac tacatcatat cctatcagca
     1741 gataaatcaa taaataccct atcaagcgaa ttggttcatt aaaatcaaat aatagcaata
     1801 aagcgatacc tgcaagggaa aataaaatcc ggaacagcgt atttctcttt ttttcatttc
     1861 catgttcaga tactttttcc ttatctattt ctgttacttt gacatctggt tctaattcct
     1921 tcactacttt ttttgtttct tgttcaatcg tagccaaatg atcttcctct gcgtcaatcg
     1981 ttagttttcc agaagtgaag tttaccgttg cttccttgac tccattgatt ttctgaactc
     2041 ctttttcgat tttcattgca cagttcgcac aatctaaacc ttctaatctg taacttttca
     2101 ttttaatgac ctcctcgcct caacatatga atatctcttc ataactaatt ataaatgaat
     2161 ggttattcat atgtcaaggt atccgaaaga aaaaatgaaa aacatctgca tcggaaaatt
     2221 tcttccatac agatgttttg gcttattctt tctttttctc ttcgatcatt gccactgctc
     2281 ttatccaacc cgcacttgcc ttcttgatct tgacaggata gcaactaagt ttgaagcctg
     2341 ttgctggcaa tttgtctaaa ttcattaatt tttcgatagg acaatatctt ttttcaatcc
     2401 ctgcaaaatg tccttcccag atatacgaag ctccactacg ctttcaaaaa tatagaaaga
     2461 gttctttaat gaaaattatt tcaactttcc ataaacagta accccatata aataaaagac
     2521 gtaaaagtca catagaagaa cttatacagg aaaacgcata gagtcaggtg aaatacatgt
     2581 atcccacctg gctctatgct ttatctaacc tttattattc tcttttatac aatttctgca
     2641 ccgaatggca tcaacgccaa tcgagcaagt ttgaaagatt gcttggcaaa aggtatccct
     2701 atgatcgtga tacatagaag gatgccgctg acgacatgtg cgagagcaat tggtaggcct
     2761 gaaacgatca gccagatgat attaacgatc aatgacatgc cagactggtg ataaattact
     2821 tctttgccaa aaggcgcaaa agaaagggag gcgatcttaa aacattgcat accgattggg
     2881 atacctatga tcgtgataca ccaaagtatc cccgctgcta accagctaag accaccgatt
     2941 agtccgccga atataaacca gatgacattt cctaaacaac tcattgtatt tctccttcta
     3001 ttactgtttt tgattaaaac tgggcaaaaa ttgattttat tcttcttttt ttagcttcca
     3061 gcatcaagta ttctgcaaat tttttttgga tttcttccgt attttctttt gttactaatg
     3121 tgtgttggat tagttttcct gatggtcgct gatccaatcg ttcttctgac tgttgcacgt
     3181 ccgcttgcgc aaatccgata atccgttttt cttttgttac agcaacgagc aatagtttat
     3241 tcttatcatt taggtcagct gccgtttttg cttttacgaa tgattctgga tgatcagtaa
     3301 tcagctgatt gatttgaggt aaatcttctt ttactgccca ccttatttcc atcatttttc
     3361 acttttcttt cgctcatttt tatctccact aaaaaaatcg actgcgacaa ttgcatctat
     3421 ccagctttgt gcaatgatct catgtccgcc aatcgttggg tggaccccat cttctcttgt
     3481 atatttcgaa aaaccataag caatccctaa agcattgaat ataccatcta aaggaaccag
     3541 caatgcgcca tattccttag caagtttacg aacaatttgg attttagggt ctaagtcttt
     3601 acgccagcca tgacggtcgt cggttatagg aagtacaaac ggctccatca agatgatgtt
     3661 gcagatttcc gctgtttgaa gttcatttag taattttcga taaacggatt caaattgctg
     3721 agcagatttc tcagttccga atatagcctg atcgctagta ttatgccagg tatcattgat
     3781 tccgatcaaa atcgatacat agtcaggttg taaagctaaa caatcttctt gccagcgtac
     3841 ttgcagatct gcaattttgt ctcctcctat cccacggttc atgcattcaa attccatcgt
     3901 tggatattgg gagattagtt ttccggcaat caaaagtgga taacctttac ctagatcagt
     3961 tgcttctgct cggtatcttc ctgcgtctgt aatgctatca ccgataaata atatacgttt
     4021 tttcatttgt caaaaactcc tttttgaata aaacgatgat tttcttcctt atattatccc
     4081 ataagcccta tcaagacgaa agagaaaata tatgtataaa aaatgacacc agcctagttc
     4141 cttcaaacaa ttgaatgaac gctcatcaag tgtctagttt tttatgctat ttttttctta
     4201 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagagatcga tcacacccaa
     4261 gcttccgtca atcagaccac tgatgacgcc atcgaaacat atatttatag gctcatctaa
     4321 gacttctagt aaatgtccct ttactaatat ctcttctaga ttgcttggga tagattggtc
     4381 ataactttat ctggtccgat gatttcaagg gtgaattgaa cttacatatg gattattgca
     4441 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtgct
     4501 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaattacc
     4561 ttctttgtaa atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     4621 atttcttttg ctttcttctc ttgtctgctg gaaacttcca ttatcccgta tttaaaaaaa
     4681 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccacaagtc
     4741 acaatagtca tcaaacatga aaaactgttt tttgaaatta ctccttgtga ctcggagctg
     4801 aacggcaggc gcacaacctc ttatggataa attcggttca acaaacgtgg gaatggaata
     4861 gcttcacgta catggtcgat accagcaatc caagtgactg ttcgttcaag acctagacca
     4921 aaaccagagt gcggaacaga accgtattta cgtagatcaa gataccatga atattctttt
     4981 tcatccaaac cgtgattacg gatttgttct agcaagaaat catagtccgt tgcccgttcg
     5041 cttccgccaa taatttcacc gtaaccttct ggtgcgatca tatctgcaca aattaccacg
     5101 tcttctcttg ttggatgtgg tttcatgtag aatggcttga tggcttttgg atagtttagg
     5161 ataaagacag gctgttcgaa tgaattcgca atgaacgttt catgaggtga accaaagtct
     5221 tcgccccatt cgatgtcatc aaatccattt ttcttcagta attcgattgc atcatcgtaa
     5281 gtaatacgag ggaatggcaa ttttgtataa gtttttaaga cttctttgtc tcgtcctagt
     5341 acatctaatg cgtattcgca atgatctaaa acattttgta ctaagaatgc tacatattgt
     5401 tcctgtactt ctaaactttc ttcttgatga gtgaaagcca tttctggttc gatcatccag
     5461 aattcgatca aatgacgtcg tgtttttgat ttttcagcac ggaaagtcgg accgaatgaa
     5521 aagacttttc cgaaagccat agcagcagct tccatgtata attggccact ttgagataaa
     5581 taagcttttt gatcaaagta atttgtttcg aatagatccg tagtgccttc tggtgcagaa
     5641 ccagtcaaga tcggcggatc aattttgaca aaatgatgtt gattaaagaa ttcatacgtt
     5701 gcacgaatga tttcatttct gatctgcata atcgcatgtt gtcttgaaga acgcagccat
     5761 aaatgacgat gatccatcaa gaagtctgtt ccgtgttctt ttggtgtgat cggataatcg
     5821 tggctctcgc ttaccacttc cacatttgta attccgattt cataaccaaa ttttgaacga
     5881 ctgtcttcac ggatttctcc agttactaac acggatgttt cttggtgtag atttttagct
     5941 gtttggaaaa cttcttccga cacttcgctt tttacaacta caccttggaa aaaggctgtt
     6001 ccgtctctta attgtaaaaa tgtgattttt ccgcttgatc gcttatttgc gacccaagcg
     6061 ccgattgtta ctgtttctcc cacatggttt ttcgaatcga tgatttgaat ttgttccacg
     6121 tatgactctc cttttatatt ccttgaattt cttcaggatt aattttgact tttactctca
     6181 ataaattgct tgattctgcg taccgcttct tctaacgtag cgcgatcagt agcataactc
     6241 atgcggacat tttctggtgc cccaaagcct tcgccagtca caagcgctac ccctgtttct
     6301 tctaaaagat cttctaccca ttgtgtgaca ttttcatatt tacacatgcg tagcgtttct
     6361 tttacattag gaaaaagata gaaagctcct tgcggctttt caagagaaac acctggcagt
     6421 tctgccacaa gcggatataa cgtattcagg cgttcttcaa aggcgttccg catttcttct
     6481 actgtatctt gctcaccttt caatgcttca acagctgcat attgactgac tgctgtcgga
     6541 ttactggttg actgggaagc aacagcaatc attccattga tgatctcaga attccccacc
     6601 gcataaccaa tgcgccagcc tgtcatcgca tatgttttag acacgccatt gatgatgatg
     6661 gtctgatttt tgattgcttc tgaaatcgtt gcaattggag taaactcatt gccattataa
     6721 actaaacgac cataaatatc atcagcaact atcaaaatat cattttttac tgcccattca
     6781 ccgattgcct gaagttcatt cttgctataa atcattccag tcggattcga tggagaattg
     6841 atgattacgg cttttgtttt ttctgaacgt gcttgttcta gctgttcaac agtaactttc
     6901 cagctattgg attcttcccc tttcacaaag acaggaagtc cttctgccag ttttacttgc
     6961 tcaccgtaac tgacccagta aggcacagga atgatgactt catcttgcgg gtcaaggatc
     7021 gtttggaaaa gggtatacaa agcaaatttc gctccgtctg ttacgactgt ttgtgaaggt
     7081 tcatagacaa gtccatagtt tttcttcaga taatcaacga ttgcctgtcg aagctctggg
     7141 attcctgctg ttggtgtata atagctagct ttcccattgc ggattgcttc gattgctgct
     7201 tcttggatgt tttctggtgt cgcaaaatcc ggttctccca cagtcaaact cagcacatct
     7261 ctgccttttg cttttaatgc cttggccttt gccgcagctg ccaaagtgac agacggttct
     7321 aatcgttttg ttctttttga taactccatc ccattcattc ctttaccttt taaattccct
     7381 tgattgtctt gacttcatct ccagattcaa agccaatcag gtaataattc agtttcccat
     7441 ttgtttcttt tcctactact tcccacacag gctgatcatc atacatccct aaagcagctt
     7501 tttcgatttg gatttcagga tgggccgcag caattgtctg ttttgcttcg ttttcagtca
     7561 gtccctttgt ttgatccaat acttttactt tttcaccaga tttaggaatg ataacggcga
     7621 tttcttttcc tttatcattt gtacctgtca gtgaaaagta tgtcttcttt cgagtaaacc
     7681 aataaaaatg atcggtttcc tcaagatttg catatttctt tgcgatttct actgcttctt
     7741 tcttcgcctg caccgccgga cgactggaac gcaagtaaaa aatcgagctg atcacgatga
     7801 ccgctaataa aaatacgatc aagccaagta acacgcgatt aatcgttttt tcctttgtct
     7861 gttttttctt caagggtcgt cgctccttta tcttcattcc ttttcattat agcagatttt
     7921 aattatgaaa aaagacagct cctctaactt ccttcttcat ttttctttaa gaattcatgg
     7981 atatcctgat agatttcctc catagctagc tctttgatcg gtactttttt cggtaatgca
     8041 tggatgatcc gtgcgccata ttttgcagtg actagtcttc gatccaaaat aagcaatacg
     8101 ccacgatctg tctccgtcct tatcaagcga cctaatgctt ggcgtaactt caaagctgct
     8161 ttaggtacgg attcctggta aaaaggattg atcccctctg attctaaata agcgttccta
     8221 gctttgacca tcgggcgaag cggattatcg aagggtaaac gtgtgactac aacgatctgc
     8281 aaagcatctc caggcaaatc tacaccttcc caaaagctgt cagcgccaaa caatacgctt
     8341 ttttctgaca aaatgaagcg tttcaaaagc ttctcccggc ttccaccaaa cccttgagct
     8401 agtatttctc gtcctttttc tagcatagac agatgcattt tctgatagac cttctgcaaa
     8461 acatcatgag aagtgaataa cacaagtatt ggccgatctt cttgttcgat cagacctgta
     8521 agtgctgtct ctaaataatc gatgtatgct tgattgctga cttgatggac ggcaggtgcc
     8581 tcttttggca catagatcct tgcctgtttc tcataatcat aaggagaggc aatcacttta
     8641 agaggtgctg cttcaatgcc aagcctctta gagagatagt gtcgattagg gccgatcttc
     8701 agcgttcctc caatataaag aatacgatca tatcgaggat accaaacagt cgacggcaac
     8761 atagccgctt ggaaatcatg gagatgaaaa atcatctgct gcgctgtgtt cccaggaacg
     8821 aaccaatgga tcaaatgatc tcgccagtct tccagccatt gatccatcag caagtactgc
     8881 ttgtctaatt gatcaaataa gctatataaa cttgctaaat cgatccgcat ctggtttgtc
     8941 cagctttctt ggataatctc aaaagtcaga cgcatttgtg actgtagctg ctgaatctcc
     9001 tgataaaaaa gctgcaggtg atcaatggct gtcccagatt ccagtggcag ctgatccagc
     9061 atctcttttg tcatcatgac ttcttctgga tactgtccgt tgggttgctt gccgctcact
     9121 aaactcatta atccttctgc catacaaatc tgtgcttcgc atagcgcaga aagttcttct
     9181 cgataaatcg ataaccagcg taaggcttca tgttctttcg gtaaatagct ttgtattcgg
     9241 tcaaacagct gatttggttc ttctaattgc tggatttgtt tatggaaata gttaatactt
     9301 aatcgtttgt catttacttt ttcagcgatt tccggcaaat gatgtacttc atcgatcaac
     9361 aaaaatggac tagctggaag caatggttct tttcgctgtg tttcttgtac taaaaacgca
     9421 tgattgacaa tcaaaaaatt actttgcctg attttctttt gcaagaaacg aacaaagtct
     9481 tgttcgtaaa acgactgatt tcttgctaaa aacccagtac ctctgtgcgc gatatctgca
     9541 aagaatgcat ggttcaagtt tgttaaatgt aattcatcca aatcaccggt cttcgtttgc
     9601 gtcagccaaa ctaagaccgc catttgatac aaagcatatt gtttttgttc tggtggttcc
     9661 acaagtgttc ctttaaatcg ttgcaggtcg atatagtggc gataactttt gataattaca
     9721 ggttgtatgg gctgatctag caattgattc agcaatggaa tttctttttc tatgatctgc
     9781 tgctgcaaaa ctagcgaaac ggtactgatg acagcgggtt tttcaggtgt agccaaataa
     9841 ctcaatggca acagataacc gattgtttta cccattcctg tagctgcttc gatcatcaaa
     9901 tcttttgatt catcttttgt gaaatggtcg tagatcgcat tcatcagtct gttctgttct
     9961 tttcgataca tcagtgtttt accaaacatc ttttcttttg cttgtttttt tcttggatat
    10021 gtccgctcgc cgtaatggac ggatgtgaat agttccactt cttttttctg caaagctagc
    10081 ccatcgacga tttctaatga ggaatcaagt ggctctggtt tttctttcat ttcttcaatc
    10141 acatgataaa tgaaacgact cgtatccata cctgtgtgtc tacttaatcg cgcaatctgc
    10201 tctaacgtga tgattggcaa ttttctcatc cgttcttcga tcagcaaaag aagttgacct
    10261 gtcacttcgg catcactatc tgcctgatga ggattctcat gaataaatcc aaggctttct
    10321 gacaaatcag ctaagcgaaa gcttggttcc gtcggcaaga aaatttgtgc aagttctact
    10381 gtatcgatgc ctggtatctt caaactagga agtccgcatc gcatgagttc atggttcaaa
    10441 aaattataat caaaataaat attatgcgcg acaaatatgg tgtctgccaa aagattgtaa
    10501 atcgtttggg cgacatcttc gaaataaggc gctttttgga cgcgttgatt cgtgatccct
    10561 gttaaatgct ggatttgttt tgaaatcttc cgccctggat gaatatcgat cgaaaaacgt
    10621 gaagtgatcc gacctgattc aatcatgacg cagccaaatt ggatgatccg atcttctttt
    10681 ggattcgtcc cagtcgtttc gatatcaacg actgcataag ttcttggttt caaaatatct
    10741 tcctcttctc ctgcctctaa tgttcttttg aaattatact acaaagtgca gaaaaatact
    10801 ctcatcgctt tttaaagaaa atagacaaaa acgcccagac acgcaaatgt ctgggcgttt
    10861 attttttagt ataggttaat taagctttag taacgtttgt tgcttgaggt ccgcgttggc
    10921 cttcttcaac atcgaaagtt actgcttggc cttcttctaa agttttgaat ccgtcgcctt
    10981 ggatagctga gaaatgaacg aatacatcgt ttccgttttc tgcagtgatg aatccaaatc
    11041 ctttttctga gttaaaccat tttactgtac ctgtttccat aataaaaatc ctccttgtgt
    11101 atcaacacat attttgcaat tacttgaatg gtgtatatct ggaagatgtt tatttgaaac
    11161 aagctaccac agattaccga acaaaaacta ctcctatagt atatcatacc gtttacaaaa
    11221 aagctagagt ttttcgactt tttttattaa aaaaagtttt ctcagcgatt acataaagtg
    11281 aaaaaacgaa ctttttttaa aataaacgac atattgttca gttggtgttt ttttgctctg
    11341 atgttcttct ttaaactaaa aaaattattc tttctgacta cttatcggtt ctgtttcaac
    11401 ggtcgaggct ggggaagatt ctttagtagg tctgatgttc tcatggtatt tctgatagcc
    11461 tatgacagtg accagaagaa caaggcaaac caatacgatc aacgagatta gtttttcttt
    11521 tttgctcata tctatacctc ataaataaca ggatgatgtc catcatattt ctgagtatga
    11581 gtaaaagcaa aagctgcagc tgtagaatgt aagaaatgcc agcaactttc ataatcacag
    11641 gtcggacagt accaaaagta accaaatctt ggctgcaaag accgttcttc tgtttcagga
    11701 atcgcagaat caagatctgt tgtctgtaca taaacatttt tgtacagacg tgtgcctgta
    11761 ataggagacc gctgtgtttc ttcagcaaaa actggctgac ttgttaaact tagggataga
    11821 aacagcaaac accataacag tctttttttc ataaagacct ccttatcgat aagtttttcc
    11881 caattaaaat catatctcaa gaacgcccgt tattcacatt ttgttcataa atgatgtttt
    11941 ttgtatcata atcgtcaaaa agaggtcgtg accacgtcac aacctctttt ttaaacggtg
    12001 ttccagaagt agcttcttcg gaaataagcc gaaatttcac aaaaatttga gaaataattt
    12061 ttgtcaatct cttcttattt cttgaagctg accacttctg tcacaacctc ttccttaata
    12121 ctcaggtcaa aacactgatt tcacaacctc ttttaataac gaagaatcgc tgtcagactc
    12181 ttttcatctg gcgcatcttt ttgatccaaa ataaacactt tcccgccatt aatgatcaca
    12241 tcatctgcta atgcattcaa cacttgacgc cgatcatatt ctgtttcagg atcttcaccg
    12301 aaaccatcag ctaagtttga cgtagcgata aataaatgtg acacttttcc ttctttcgca
    12361 gctggaacga tatcaactaa ctgatcgatg aatttcttat ctaagagttt attgtattct
    12421 tttgattctt gagctgcgag ttcacttgac agcttcttcg ccccttcttc gatatctcta
    12481 aaggatagct gcgcaggaga agcagaaatc gcaacgtctg cacaataata gggattttta
    12541 gcaatttttt tgaacatcgt ctgattttct ggtaatgcaa ataaataaat cggccattgt
    12601 tcaggatttt cgaaagaatc ctttaagtac gtatcaacag cttggtaata gttgacccaa
    12661 tcgatctcta cttcttcatc ttttggattt acgccatgga aagttactcc atctcgggct
    12721 ccgcctcctc cattagaaga gaagttcaaa ccgccacctg tcagttcaga tcccaatgca
    12781 gtaacgacat caaccggtgc cccttctggt agagatactt cttttacttg tttgttgctg
    12841 acctcataca atttcatgga gtcgcggttc aatcccaata aataataaga atagttgaac
    12901 tgcccatttt tgataatagc cagtaaataa ggaagatcac ttacatagta ttgatcgtct
    12961 accgggatac tcaaacgatg gatataggtc tggtcagcag ttataatgac cgctacactc
    13021 gtagttgcac tacgccaaaa cgaaggatct cctaacaaat tttcaatttt ttcttggaac
    13081 ggagaccagt catgttcgga atattttttc tcaaaacgtt tctttgcttc tttcgcaaaa
    13141 tttttgaatt tgatctgatc tttttccaca tcttgatggg cgacatgcgt attcagcatg
    13201 atcgtgacga atggtccttt agcgccttct gaagtcaatt gtgtcaataa ttctttatcc
    13261 ttttttgcca taaacgtacc cctcctaaaa aaatcattcc tttgtttcta tctaaagtct
    13321 accacaacac tatttttcca tacaataaaa cgtatctaaa aaaataaaac ggtttctacg
    13381 gttttaaatt tagctatttt tttaagttat cttcataatt tcctcaatta tttagcccca
    13441 gtcttcaact attcttcatg aaatgctgat aaactaagaa aaagaataaa agaggtggat
    13501 cttatgcatt taattatgat tttcagttta ttaagtatta tcagtttatt tgctttcagc
    13561 gcttcccgct tattagatgc ggaaacacgg cctattcctg taaaagtacg taaaaacgac
    13621 cgctaaactt ccataacgcg cgttcttatc ctaaagtttt ttcagaaaca ttgaaactct
    13681 ctcccctctc tgataaaatt ttaatttgta gcgaaacaaa ttaattacaa ggagatcgga
    13741 tatgtccata aaaaaaacac gtctagtcct attattggct agtctcattc tttgccttgc
    13801 ttcttttcta ccagctgttg ctgtgcaagc tgaagatacg ttcaaagtaa atgcaaaagc
    13861 agcttttgct gtagatgcgc aaagcggtaa gattctttat gatcaagatg gagaaaaaac
    13921 catggggatc gcttcaatca caaaaatcat cggtctttac atcgtccttg atcaagtaaa
    13981 agaaggaaaa ctgtcatggg atgataaagt ttcgatttca gattatgcag aaaatttgag
    14041 tatcacccca gatttatcga atgttccttt gcataaagaa aacacctaca ctgtcaaaga
    14101 attattcgac tctgcaatca tccagtcagc caatgcttca atggtggcct tggcagaaaa
    14161 aatctctggc agcgaagcta aattcactga acgaatgaaa gaacaattga aagattgggg
    14221 aatcaaagat gcaacaatcg ttaatgcatc aggattgaac aactcttatc ttggcgaaaa
    14281 ccgaccagaa ggcactggag aaaacgacga aaatcaaatg tctgctcaag atgtggccat
    14341 cgttgcccgc catttgatct tggatttccc agaaatactg gatgtatcaa gtactacgac
    14401 ccaaatgttt ggcgaaaata cgcaatcacc agtggaaatg gtcaactgga actggatgct
    14461 cccaggattc gttaattaca aggaaggcgt ggacggatta aaaactggta cgactgagct
    14521 tgcaggtgct tgtttcgttg gtacgatcac taaaaacgat caacggatca tcactgtcgt
    14581 attgaatgcg gacggccatg cggaaaaccc gagtgcccgc ttcaatgaaa cagaccgttt
    14641 gatggactac tgctatgaca attggtcaga aaaagagctc ggaaaagcta acgcaagtat
    14701 tcctagtcta aaaaccattg atgtaaaaga cggaaaagaa tcaacagttc ctgttgtgct
    14761 taaaagccct gtaaaagtat gggtacgaaa cgatatggat actggcaaat taacgatcac
    14821 gccaacactt gacaagaagc aagtcactga caatgagatc caagcaccta tggataaagg
    14881 tacaaaaatc ggtaccgcta cgatcacctt ggcagacgat aaattaggct atttagaaga
    14941 caacaatgca ccatcaacag agatcattac ggataaaaca gtggaaaaag cgaatatatt
    15001 cgtgattggc tggcgacgcg tcgcagaatt tttcgacaac ttgttttaat tgaacaataa
    15061 gaaaaacatc tttccatcgg aaagatgttt ttttgctttt tctagtttaa ataacagctt
    15121 ttttcatcta ttttcgttat actgtataag aaaaaggagt tgaatttatg aatctaacca
    15181 gagatctgat ctttttagtt ctctatctta ttggggtatt ctctatcgtt tattggcgaa
    15241 aacagaagaa ggatccttcc ttgttctttt acggcagtac gtggacattt acggtaatct
    15301 taacttattt tgtcgtgttg gaaatttttt attcgacctt ttattttttg attccattgt
    15361 tgttctttag tatctttgct ttttcttatt tcacagaaaa acgaaggctg ctgaatggtc
    15421 ttttattcaa tgttttcctc atcagtttcg gcatctactt gtttgtcttg ctgtatgaaa
    15481 cgcaaaatat tttccttggc ggactgatcg ccttgatcac gattccattg ctacttgtcc
    15541 ttttatttgg tatttatgga ttgattgttt ttcttttttg gaacggggtc acagttttac
    15601 gtagagaatc gcattcatta gctaatcttt taacgttgat actagccatt tttcttacac
    15661 tgtttcttgt ttttgacttt ttcttgttaa aatatttgcc gcaatggata aatgctttgt
    15721 ttttctgcgt tcccctgatc ttgatctatc tatttatcgt attctacaac tttttgactg
    15781 tctcattctt gtatcagttc aaccggcctc gatacaatca agacttcatc gttgtgttag
    15841 gcgctgggtt gatcaatgga gaaacagttt ctcctcttct agccaagcga atcaataagg
    15901 caattgcttt ttatcgggct caaagccgtg ctacattaaa tccgcccatc ttattgatgt
    15961 ctggcgggca aggagcagac gaaaaagtac cagaggccat tgcgatgaaa caatatgcca
    16021 tggaacaagg aatccctgaa agagacattc ttgtcgaaac caattcgaca accactttgg
    16081 agaacatgct ctattcaaag gaaatcatgg atcagcaaat gaaagggcca taccgcgcga
    16141 tctttagttc caacaattac cacattttta gagccggtct ttatgcgtgg caagccaaat
    16201 tgaaagcaaa tggtattggt gcaaaaacag ctttctacta cttgcctaat gcgtttttaa
    16261 gagaatacat cgccatcttg atgatgaaca aaaaaagaca tatgatcatt tgcggattgc
    16321 ttttcgcagg ctccgcattc ttatcgctga ttactttact tttctaaaaa aacaaggtgt
    16381 gacaaaacat atgtcacacc ttgttttttt ctttcattct cttaactatt ggcaaaaaga
    16441 gtatcccttt tttgaccgct acttgctggg cagaataaat accgctgttg cctgaacaca
    16501 taaagctgat caagcagatc ataaagaagt atactgccgc ttgtgaaccg aacaattcga
    16561 tccccatcac aaagcaggca atcggtgtat ttgtcgcacc agaaaatact ccgataaagc
    16621 ccaatcctgc aagaaaagga acagaaacat gaagcaaagg cgctagtgct gcaccaagcg
    16681 ttgcaccaat ttcaaacaat ggtgtcactt ctcccccttg atatcccgcg cctaaagaca
    16741 aaactgtgaa gaataacttc ccaataaaat catagacatg tgcctgtcca ttaaatgatt
    16801 cttgaagtaa cggaagactc aatccgagat aacgttgtgt ttgcaaaatc aatgctgctg
    16861 ccacaactac agctccacct atcgtatttc gccaaacagg atgaggaatc cattgtgcat
    16921 atcgcgcttt taaaaagacg atcgaacggc tgaataccca accaatcaaa ccaaaagcaa
    16981 tcccagcaac aaaaactttg aagaacagca acatactcca ttcaggaatc acacccattt
    17041 ggtaatgtag atgagaaaca ccatagcttt ctgtcatgaa atttgcaaac agtgcagcaa
    17101 agaagctagg gaagatcgct tctgttcgga ctttcccgat tgccaacact tctaatccaa
    17161 ataatgttcc agctaaaggt gtgccaaata cagaactaaa acccgcactg attccactga
    17221 taatcaagat ttcccgttct gctttatcca atcggaacaa acgtgctgta ttatctgcta
    17281 acgatccccc catttggact gctgttcctt cacggccaac agacccccca aataaatggg
    17341 tagttatggt tccaaacagt gtaagtgtaa taagccgcaa cgggattttt tcttctccac
    17401 catttccttg atcgatcact aagttgtttc cacgactaga aagaccgccg tgatacgcat
    17461 agagataagc aaaaagcgca ccactaactg gtagtagata aaggagccaa ggatgtgata
    17521 gtcggatatc tgtcacaact gtcaggctat ttaagaaaaa agcagacaag ctccccatga
    17581 atataccgag aatcgctgtg atcaccagcc acttcagcat gtacactaaa atgcgcattg
    17641 gactcggtaa ttgtttttcc ataaaaaatt cctcctcaat aaaaaaacgc ctactaaaga
    17701 aaggggctgt ccccagactt tagtaggcgt cattagctat ctttcgttag catttaaagg
    17761 cgaacaccat cacctattca cttccattaa aagatagcat atttttctgt aaaaaacaat
    17821 aaaattatcg ttctttcttt acacgttcag caaattcata gaaaggagcc aatttcgtta
    17881 actgttgatt acattggtca ctatcatgac aaatataatt cccctttttc gtatacgtgc
    17941 catcagaacc tgctttcgtt gtagaaagaa aaagcgacac attcgatgtt ttctgacaga
    18001 ttgcacagat tcccttattt atcgttggtg ataaagtacc gctaatccct tttaattttt
    18061 catcttcgta atagaccatg aattttttct gcgtacctgg atcattccag ccgatgtacg
    18121 tatgttcttg caagtcgatt tcattccaag ctggttgttt gagttttttt acttttcgga
    18181 ataatttctc gatttgtggt ttgctaggct gcataaacgg ctgaacgatt tgcttcagct
    18241 tctccaatgc tctttctgct tgcgcttttg tcagttttac gtctaaaact gtcgttaaaa
    18301 atgtatcgat ttctggcagt tcgtgattta atatgtctcg aatcttttca gctgctagtg
    18361 cctgaactgt ttgtaccgtt tttggatcat tgacagatga gtacgcattt aataactgtt
    18421 ctacttctct ttttataaaa aaatattggt atggttttat tgtttgttcc atgattttaa
    18481 agtcctttct tctaaccaaa gtgcatactt tgtagaaaaa agtgatcttc tgcaaagtat
    18541 gtgttacctg aagatcccgg actcttttaa ataagatacg ttttgcatct tatcatcacc
    18601 ccctttaatg cttattcttc tatgtacatg attcattcga taaataaaag taaaccgtat
    18661 cttgaacaga tagtcaactg caaacagaaa aaaggactgt gacaaaagtc gtgtcaaagt
    18721 cccttgtttc gaatgaatgg tgttcaaaag cttgcatctg cggtaataag ccgaaa
//
LOCUS       DACEKH010000055        18329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEKH010000055 DACEKH010000000
VERSION     DACEKH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.056"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(121..687)
                     /locus_tag="IW662_001730"
     CDS             complement(121..687)
                     /locus_tag="IW662_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0866554.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(687..1643)
                     /locus_tag="IW662_001731"
     CDS             complement(687..1643)
                     /locus_tag="IW662_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ0866555.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1643..2299)
                     /locus_tag="IW662_001732"
     CDS             complement(1643..2299)
                     /locus_tag="IW662_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0866556.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2669..4687
                     /locus_tag="IW662_001733"
     CDS             2669..4687
                     /locus_tag="IW662_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0866557.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5114..7528
                     /locus_tag="IW662_001734"
     CDS             5114..7528
                     /locus_tag="IW662_001734"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ0866558.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7876..9429
                     /locus_tag="IW662_001735"
     CDS             7876..9429
                     /locus_tag="IW662_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0866559.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9810..11594)
                     /locus_tag="IW662_001736"
     CDS             complement(9810..11594)
                     /locus_tag="IW662_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866560.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11609..13330)
                     /locus_tag="IW662_001737"
     CDS             complement(11609..13330)
                     /locus_tag="IW662_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866561.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13430..14191
                     /locus_tag="IW662_001738"
     CDS             13430..14191
                     /locus_tag="IW662_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0866562.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15158..15426
                     /locus_tag="IW662_001739"
                     /pseudo
     CDS             15158..15426
                     /locus_tag="IW662_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16098..>18329
                     /locus_tag="IW662_001740"
     CDS             16098..>18329
                     /locus_tag="IW662_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866563.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6014 a         3271 c         3709 g         5335 t
ORIGIN      
        1 ttattaccgc agatataagc ttttgagcac cgtgaataaa gaggttatga aatcaacgct
       61 ctaacctgag tattagagaa aaaaacgcaa aacatatcat ttatgttttg cgttttttat
      121 ttatcttttt ttctcaatac aatataaaat aggcggctgg ttcttttggt tgataaaccg
      181 ataattcagc acattatatg tttcctgtgg cagtttggaa caaaaagaat caaccatatc
      241 cagttctttt tctccacctt catgaccata gtagacgaca aggatcaatc gtccgcgagg
      301 cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta gtctctggca tagtgatgat
      361 tgctttatca ctttttggga gataccctag attgaaaatc cctgctttga gattttgttg
      421 ttcagctatc acttctccta acgtttcgtg gccttgtaga aacaatgaga cacgttcttc
      481 taactgacgc tgttctagcc tttcacgagt agcatcgatt gcttgttgct ggatatcaaa
      541 actatagaca tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat cgtggccatt
      601 gcccatcgtt gcatctacga cgtgatcccc tggttgcaag atttcttgca gtaatgtatg
      661 gctgaaatgc aaagcagttt gcaacatcag acgctcacct ttttccgtac atcatatttt
      721 ccttggaagc tatctctgcg aagaagttct tcatcaattg cattcaagac ttcccatttt
      781 ttcaagctcc acattggtcc aatcaaggaa tcccacggcg catcccctgt caaacgatgg
      841 atgatgatct cttttggtat catttccagc tggtcgcaaa tgacattgac atagtcagga
      901 cggctcatta attgaaggcg accttcatga tagtcccgca acattctcgt tttgcgcatc
      961 agatgcatca aatggagctt gattccttgg atatctgaat ctaaaatggt gcgtctgaca
     1021 ttttctttca tcatttctaa actttcgcct ggtaatccgt tgatcaaatg cgtacaaaca
     1081 cggatattat gtttgcgtaa tttagccacg ccatctaaat aagtttgata atcatgtgct
     1141 cggttgatca gttttgaagt ttcttcaaaa gttgtttgca atccaagttc tacccataaa
     1201 tacatccgct cgttcagttc agcaaggtaa tcaactactt catctggcag acaatccggt
     1261 cgtgtaccga ctgataatcc tacgacacct ggtaaattga ctacttgctc aaatcgttct
     1321 ttgattattt caactggcgc atgggtgttt gtaaaatttt ggaaataaac gatgtattgt
     1381 tggacatgag gccatttttg atgcatcatg tcgatttctt tctggaattg gattggaaga
     1441 gggtcttcag gtgcaacgat catgtctccc gaaccagata cactgcaaaa agtacaaccg
     1501 ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc ctccatcgat cggcaccttg
     1561 aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc aagaatgata tcttttattt
     1621 ccttctgaat aaggaaaagt cattaaaagt ccttctttct gtaagagtta tctatttgtt
     1681 ttcttttttc tctatacact ggataagtca ggcacagcca agaaagactg agtaagtagc
     1741 cgccgatgat atcagtagga aaatgaacgc ccagataaat gcggcttatc ccgacactta
     1801 ctataataaa catcatcagc aattgaagca atgtgcgtaa ggttcttttt tgaacaaaag
     1861 ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca tgtccactag
     1921 ggaaactgta acttgtttca actactaagt gcgccaatga aggacgctct cgcatgaaaa
     1981 atagtttgat caccgggttg atgattccag caattacaat aaatccagaa acgaaccaaa
     2041 atgtttctgc cctttttttc ttataccaca tccataaagc cgcacaagca aataaaatga
     2101 tgacggtaag aggattaccg aatttagtca taaagagttg gtagctgttc agatcaggat
     2161 aagaagctct gacaatgcct gtaaaccact gatcgaaacc agaaagccac gacagatgga
     2221 actttacgat ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca gccacctgaa
     2281 atgatgtttt atatttcata caattcctcc tttgaagcaa tgacttatta tagcattttt
     2341 ggtccagcga aaaaaacggt aaagagataa aaccaatgta tgattcctct ttgaataacc
     2401 aatattctta taaaaaaaat aactgtaatc ggaaagacat tttttggtaa tgcaaatgaa
     2461 atgttaggga aataaaatca gtgtttttcg aaatcctttt aaacaagaac gtagacgtag
     2521 gaagaacttg aaaatgaaca gaagttgttc attacggtaa attacaatat gattacaaaa
     2581 aacaacaaag ctcttaaata acttttaata aatattaaga gaagttataa ttattaataa
     2641 tagataaatt gaagttggag gatagaagat ggaaaacatt gcaagaaaag aacggagacg
     2701 tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg gcagcaatcg tgggaactgc
     2761 gatggtaggc tgttctgtcg ctgccccatt agtacagccg gttcctgttc aagcggatga
     2821 aacacctgtt cagttcggcg caagaatcaa tacagcggca tttatcacag aaattgctac
     2881 ttatgctcaa ccaatcgctc aagccaacga tctatatgca tcagtgatga tcgcacaagc
     2941 agttgtagaa agcggttggg gcagtagtgc gttatctcaa gcaccttatt ataatctttt
     3001 tgggataaaa ggcagctatc aaggacaaac cgtctacatg gatacgcttg aatttttaaa
     3061 taatcaatgg gtaaataaaa aggagccttt ccgacaatat ccttcttttg ctgaatcttt
     3121 caatgacaat gcctatgtac ttagaaatac ttcatttggc aacggttatt actatgccgg
     3181 tacttggaag agcaacacga aatcctatac agatgcgaca gcttgtttga cagggcgtta
     3241 tgcgactgat cctggttacg caggcaaatt aaataatatc atcgcaactt acggattgac
     3301 ccaatatgat acaccagcta gcggaaacgc aggcggtgga gcgactacag gtggcaataa
     3361 tactggaagt gcaggtagta ctggcggttc aagcaattct ggtaatacaa actcaggttc
     3421 agcaacttcg accacttata ctgtccaatc tggtgattct gtttggggaa tctcaaataa
     3481 attcggtatc acaatggatc aattgatcca atggaacaac atccaaaaca atttcattta
     3541 tccaggacaa aaactgacaa tcaaaggtag ccaggaaaat ggctcttcga cgaataattc
     3601 cggtaacaat accaactctt caggaaatgc tggtacatca aacggaggac aaacaactgg
     3661 tgcgaaatac actgttcaat ctggtgattc tgtttggaag atctcaaatg atcacggaat
     3721 tacgatggct caattgattg aatggaacaa tatcaagaac aatttcgttt atccaggaca
     3781 acagctaatc gtatcaaatg gcggttcttc taatgctgga gcaaatactg gctcaaatac
     3841 tgggaataca agtggctcta gttcgtccag ctcaagttct tctaatacag cttcaggaac
     3901 gaaatataca gttaaagccg gagactctgt ttggagcgtc tcaaataaat acggaattac
     3961 gatggctcag ctaatccaat ggaacaatat ccaaaataac tttatctacc caggacaaca
     4021 actgactgtt tcaaacagtg gatcaacaaa cagcacgtca ggtaatacag gttcgtcaac
     4081 caatacaaat aacagcagtt cagttaatac tggcagcagt tcgtctcaag gcacttatac
     4141 cgtgaaagca ggtgaatctg tttggagcgt ggcaaataaa aacggcatca cgatggatca
     4201 gttaatcgaa tggaataata tcaaaaacaa cttcatttat ccaggacagc aattgatcgt
     4261 taaaggtgga acttcggcaa atacaaatac aggttcaact acttctgcag cgaaaccaaa
     4321 tacaccgaat acgtctgcta cgacaagtac atcatccact ggtaatacga tgtataccgt
     4381 gaaagcgggc gagtcagttt ggagcgtagc gaacaaacat catatcacga tggatcagtt
     4441 aatcgaatgg aataatatca aaaataattt catctatcca ggacagcaag tgattgtgaa
     4501 gaaaggttca tctcaatcaa caaaccaaca atcagttact ggttcgaaaa cctatactgt
     4561 gaaatctgga gaatctgttt ggagtgttgc tgacagtcat ggaattacaa tggctcaact
     4621 aatcgaatgg aacaatatta agaacaactt tatttatcca ggtcaaacgt tgattgtagc
     4681 taaataaaat agctgttcga agaaataacg attgaattcc acaaaaatat ttgttactat
     4741 aaggctactt aaatatgaca acacttgaaa aggaagtagt aaaaagactg aagtttcaga
     4801 gagcgcatgg ctggtgagaa tgtgcacgga tgcttttgaa ctcgcctttg agttgccctt
     4861 gctgaatacc cgagtaagca agcggcggtg acggccgtta caacgttcga ggccttttca
     4921 ctaaggtaaa attaggtggt accgcgtaga ttacgtccta taaaaagagc aatcttttta
     4981 taggattttt ttatttcttt tgtgaagttt gagaaaacaa agcaaaagga aataaaaata
     5041 aacagattct gccggttttt tcgttatatt tataatagaa acaagtgttt tcaatagaac
     5101 aggaggacag caaatgagtt acaatcataa agaaattgag aaaaaatggc aaaaatactg
     5161 ggcaaaaacg aatactttca acacccatga tgatcctgaa aagccaaaat tttacgcact
     5221 ggatatgttt ccctatccat ccggtcaagg gctacatgta gggcatcctg aaggttatac
     5281 ggcaacggat atcctttcac gtgtgaaaag aagtcaagga tacaacgtgc tgcatccaat
     5341 ggggtgggat gcattcggtc tgcctgctga acaatatgcg ctagatacag gaaacgatcc
     5401 agctgagttt acccaaaaaa atatcgaaac attccgacgc caaatcaatt ctttaggatt
     5461 tagttatgac tggaatcgcg aagtgaatac aactgatccg gagtattata aatggacaca
     5521 atggatcttt acgaagcttt atgaaaaagg tctagcttat gaagcagaag tagcggtaaa
     5581 ctgggtacct gaattaggaa cagttatttc aaatgaagaa gtgatcgatg ggaaaagtga
     5641 acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa tggatgctga aaatcactgc
     5701 ttatgctgat cgcttattgg atgatctaga attagttgat tggccagaaa gcattaaaga
     5761 aatgcaaaga aattggatcg gtcgttcagt aggagcaaat attgaattta aagtggctgg
     5821 tacggataaa tcctataccg tatttacgac acgaccagat actctattcg gtgcaactta
     5881 ctcggttctt gcacctgaac tagatcttgt tagagaaatt acgacccctg aacaaaaagc
     5941 tgctgtagaa gcttatatcg aagagacagc taaaaaatca gatctaaaga gaacggatct
     6001 tgcaaaagaa aaaacaggag tatttactgg tgcttatgca atcaatccag ttaatggcaa
     6061 ggaaatccca atctggatcg ccgattatgt attatcttca tacggcacag gagccatcat
     6121 ggctgttcca gcacatgatg aacgtgactt tgaattcgct caaactttcg gactagaaat
     6181 tcttccagtt atcgaaggtg gagatgttga aaaagctgct tacacagaag atggtacgca
     6241 catcaactct gaatttctag acggcatgaa caaacaagaa gcaatcgata aaatgaatga
     6301 atggctagaa gaaaatggcg tagggaaaaa agaagtcagc tatcgcttac gtgactggct
     6361 attctcccgt caacgttact ggggtgaacc aatcccaatc atccattggg aggacggaac
     6421 agtcacagct gtcccagaag aagagctacc attacggttg cctaagacaa aaaacatcaa
     6481 gccaagcggt actggagaat ctccattagc aaatatcgag gagtgggtca atgtcgttga
     6541 tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca atgccacaat gggcaggaag
     6601 ctcatggtat tacctgcgtt atatcgaccc tcataacaaa aaagaactag cagattatga
     6661 aaagttgaag cgttggctac cagttgacat ctacattggt ggagcagaac atgcggtact
     6721 tcatttacta tatgcacgat tctggcataa attcttatac gatatcggtg ttgttcctac
     6781 aaaagaacca ttccaaaaac tatacaatca aggaatgatc ttaggcgaaa acaacgaaaa
     6841 aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat gtggtcgaaa cttacggtgc
     6901 agatacactg cgcatgtacg aaatgttcat ggggccatta gatgcttcga ttgcttggag
     6961 cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt gtatggcgtt tgatcgttga
     7021 tgagaacaat aaaatgcgtg accgtatcac tacattgaat gatggcaagc ttgataaagt
     7081 gtatcatcaa acagtgaaaa aagtgacaga agactatgaa aatcttcatt tcaatacagc
     7141 aatctcccaa ttgatggtct ttatcaatga agcctataaa gtggacgctc tgccttatga
     7201 atatatcgaa ggatttgttc aactactcgc accgattgct cctcacatgg gtgaagaact
     7261 atgggcaatc cttggaaatg atggtggtat ttcttacgca ccatggccaa catacgatga
     7321 agcagcactt gttgaagacg aagtagaagt tgttttccaa gtaaacggta aagttcgtgc
     7381 gaaaagcaat gttccccgag acttaggaaa agatgagcta gaaaaagcag cattagccaa
     7441 tgaaatagtg caagaatata ttgaaggcaa aacagtcaga aaagttattg cggtaccaaa
     7501 caagttagtc aatatcgttg ctaattaatt ggctctttgt caataaggac tgatgagttg
     7561 tgcaaaatca aatctgggcc agaatgaact tcattctgtc tcagattttt tgttatttca
     7621 aattgattaa ttgattgatc aaaaaggttt tgattttcat gttctcaaac acgttcaatg
     7681 tctttttctt ctgaatggtc ttgtaagaga tctctgtaat catcaaattt ttgtctatta
     7741 atcttagtat tttttgatgt ataaactacc tgaaattgag agtacatacc aaattcataa
     7801 attttttatt agtgggacta gtgattgtat ggattttgac gcataatgaa tgaagtatga
     7861 gaaaaggaga gacaaatgga tcaagaacta tttattatcg aagtattatc agctaaacaa
     7921 aaaattatcg agaaagcgaa aaaaagagcg aaaagaataa tgttcaagct tttaagctta
     7981 gtatttttct ctggtttgct ttttttcgga gctgtatcgt tcctatatcg ccatatgagt
     8041 gcagtagtcc gttcagctga agggatgcaa aaattaaata atttgtatca cttttcgtgg
     8101 ggagttggcg gaataacaat tgttgtgctt attgtttttc ttctacttat ttggctccgt
     8161 taccatcaat tgtcgcgtca tttcgatcaa tggcttagtc aaagagcaca acagttttta
     8221 cataataaac caacttctca agatgatgac tatctttatt ttgagaagac ttcgacgagt
     8281 caagcttttc ggatacaaaa aagtgattgc cagttattgc tgagatcact tgatgattta
     8341 cctatctact taggcgaaag caaatcaaaa ttcggaatga acacattcca tattttttta
     8401 ctagaaaacg aacccagatg ggaaaaaagc cagtcttcgc ctggtggata cctgacaact
     8461 aagatgagta cattagtcac tggttttttc ctcattcttt tagctggaac aatctattat
     8521 gggagataca ctcagccaaa aatgtcttct catataaatg ctgcttctca catgacaatg
     8581 aactcaacta acgatacgca tatacaaaag acaaatgaag ggttgttagc tgatcctaag
     8641 caaaaggtaa atttgacaac acaaaagata aatgatctca aacttgatac agagacgcac
     8701 tctctctata tgacaacaga tagtggtgct aattgggtat ttgtgccgat tgattcgcaa
     8761 tggttgcgtt ttggcgaata caccctgaca acggggctga ttcctgaagg ttattggatg
     8821 gacaacacat atgatatttc cgccgatttc tcatggttta tctattcttc tggggatgat
     8881 gtacgtctgc tgacttcaag agataatggt aaaacatggc aaacgtctat tatcacccaa
     8941 tcagttcagc cgatacgtta tcgaaaagcg gtattttatg gcaacgatgg tctatttatc
     9001 tgtacatcag ccagtgggat gtctgcagaa actttgtaca tttacactac tatggatggc
     9061 ggaatcactt ggataaaaag tggaagtaca atgatcaatc agccagtaca aaatgtcagc
     9121 ttcgttacaa cttctttagg ttttgtttcg acacgtgaaa gtttgtttta tacaaacaac
     9181 ggaggacgct cattcaaaga atcagttctg tcgattccgg aaaactatca gacagggggg
     9241 atagatcttt ttcaatcacc aaatgaagtt actcaaataa gcgcgaatac gttagagaca
     9301 aaattttatt tgttgaaaaa aggaccaatc gatgcaggga aaatgtttgc ctgcctcttc
     9361 cgttcaactg ataacgggga aacatggcaa ttcgttcagc aactatcgca agtagaacaa
     9421 ggagattaaa gaagagagat tgtgagcctg acgttcagca ccgagtcaca agaaacaaat
     9481 tcaaaaaaca gcttctcata tttgttgaat agtgtaactc gtgattcgga gttgacaaga
     9541 gcaacaagaa gcgattttga agtatagctt ctcgtatttt acaagatcgt gtcttattgc
     9601 cgataggttg tctcttgaac accgtttatt cgtaaaaagg gattgtgaca agtcttttgt
     9661 cactacttac cgaacgaaac tgaaggagct atccaaaaag tatcattagt attattcttt
     9721 gtgtttctag tggcaccact agtacattta aaaaagctat cgatcatgga ctaatcatac
     9781 atgatcgata gctttttaga taatgtttgt tacgctatct gaaattgact attataaaga
     9841 ctggcataaa gcgttggtga ttggagtagt tcagaatgtg ttcctttttc gacgatatcg
     9901 ccattcttca ttacaaggat catgtctgca ttttcaatcg ttgacaaacg gtgtgcaatc
     9961 acaaaacttg tcctatcttt tgtcacagct tccattgctt gctgaatcaa tgcttctgtt
    10021 cgtgtatcta cgctagaagt tgcttcatct aaaataacga caggaggatt cgctagaatg
    10081 actcgagcaa ttgtcaataa ttgttgctgt ccttgagata acgtgccgtt ttcgctagaa
    10141 atgatcgtat catatccgtc tggcagtgtt cgaataaagt gatcacattg tgcgatttca
    10201 gccgcttcga taatttgttc tcttgtggca tccattttcc cgtaggcgat attatcagct
    10261 accgttcctt caaacagcca ggtattttgt aaaaccatcc caaaagaacg acgtagttct
    10321 ccacgtgaaa gcttggtaat atcaagaccg tcgaattcga tctttccttt attcagttca
    10381 taaaaacgca tcagcagatt gactaaagtc gtttttcctg caccagttgg accaacgatc
    10441 gctaccgttt gttttggctt tactgaaaaa tcgacttgat tcatcaatat tttatctgat
    10501 gtgtaaccaa actgtacatt tttgaaagct atttttcctt tcggttgttc taaaagtctt
    10561 gcatctggaa ggtctggaat ttcatctgtt tcatctaata tctcaaatat cctatcgatt
    10621 gctgccattg cggattggat cgagttgatc acataagaag cggtagaaat tggttcagaa
    10681 atctgattga tgtactgaag ataagcttgt aagaagccga tcgtcacacc acctgataga
    10741 acaagcatag caccgaatac cgcacttatg atgaaggcga gttgattaat gaaacgaatg
    10801 gccggataga tagcacagtt cataaattga gcacgtttaa aagcgttgta ttgcttttta
    10861 tttacttcgt gtatagcagc ttctgcatga gcttgctgat tgaatgtttt gaccactaga
    10921 ttaccagcta agatttcttc cattcgatta tttaaaacac ccagttcata ctgattctgt
    10981 tcagaaatct gtttgttttt gttcgcaatc ttagttgtaa caattgaact gccaacgatc
    11041 agcaataaga cgagcaaagt aagcttgctt tcgatataaa acagcatcag accagcagca
    11101 gctattgtta cgacagaagt aaaaaattga ttgatacctg tcaataacac ttgagaaagt
    11161 tgattgagac cggtagttgt ccgactaatg atgtcaccta cttgatgatt gtcaaaaaac
    11221 gacatcggta atgttttgaa tttttttgtg atttcttttc gcaccctcaa tgtgatcttt
    11281 tcgcttaacg ctgccattgt ccgttcctga ataaatgaag tgatgctgct taagactgaa
    11341 cagccgatca gcaataagac agggaacaac aatgcttgtt tgatatcact gaaagaaagc
    11401 tgtccaatac ctgtttttct tatttctgat aagagatcat cgatcccgat tcccatgatc
    11461 aagggcatgg ctacgactaa taggttcccg aataaactgc atagaaacaa tccgtaaaaa
    11521 tatttatgtt caggtttgat cattttccaa aaacgtatca aagcacgcgt gttcaacgtt
    11581 tttttctttt ccatcgtatc ccttctttct atattccttg tgattttgca aactcctgat
    11641 aagtttggca attagacagt agttcttcat gagtaccttg tccaacgatt tttccttcat
    11701 ctaaaacaat gatgttatct gcttgttgga ttgtactgag gcgttgagca acaatcacga
    11761 gagttttctc cgacatgtgt ttatgaaggg ctgctcttaa tgctgcatct gttttatagt
    11821 ctaaagcgga gaaactatca tcgaatacat agatctctgc tggtttgatc aacgctcgag
    11881 caatacatat ccgctgtttc tggccccctg aataattaga accgccttga gcaacaaagc
    11941 tgtccaatcc gtcaggaagg gtgtccacta gttcttctaa ttgagcgatc ttgatcgctt
    12001 tagacaactc ttctgttgag gcatattcat ttcccatctg caagttgctt aaaattgtcc
    12061 cgctgaataa gaaagccttc tgagggacat aactgatgtg ggagcggatc tgttcttgag
    12121 tcatttgagt aagaggaata ccaccaaggg ttacttttcc tatcgtaggg tcactaagtc
    12181 tcaagagaag tttcgccaaa ctactttttc cagatccggt cccgccaacg atcgctgttg
    12241 tttttccttt aggtattgtg aaatcaatat cttctagaac tggatcagag atttcagtat
    12301 atttaaatgt gacatgttca aaagcagctg catttgtctg atcaaaggtt ggttcagcta
    12361 ctgttttgat cgtctcttcc tcaatttcga tctctgcttg cagtatttct tctatccgtc
    12421 gtaatgaggc aaacgattta gggataatga ccaataccat agctgcgatc atcaagtaag
    12481 caagtgtcag catagagtat tcgactacag cagtcacgac accgatttgt aatgctcctt
    12541 ctgctacaag atttccgcca aaccacaaaa tagaagcata aacgattccc atgagcaaaa
    12601 aagcaattgg agtgacccaa gcaaatattc ggttcacttt gatcatttga ttagcatagt
    12661 cagcaaagct cttattcgtt cgcttttctt catatgattg attatcaaag gcacggatca
    12721 tattaatccc agtaaagaat tctctcaatg tgatggtgat ccgatccatt tttggctgga
    12781 tactcaaaga taaaggtgcc cctcgtttca tcagtcgata aacaatccat acataaaaaa
    12841 caacagagat caatgtaacc catgttagta atagagaata acgaaacatc atgagaatgg
    12901 tgaaaacgca aataaccggt gctggtaaga tcatttggca aaataaaaca atcatttgct
    12961 ggacattatc gacatcattc gtcattcttg tcaaaagaga tccagaacta aaacgactga
    13021 tatccttcat tgacagctgt tggaatttac ggaaatattt ctcccgcgtt tggaatccaa
    13081 acctagcgga agccaatgct gaaaagtaac tagaggcaac tgcagcgatt gttcctagaa
    13141 acgctacaag cagcatttca actccaattt tttttataac tgaggcttcg ccacttgcta
    13201 tacctgtgtc gatcagcttt gctactaatt ttggtacacc taatgtaccg gcgatttcta
    13261 aacagatcat cacaaaggta aggagagcga gtcctttgtt tttctgtaag aatgttttta
    13321 agatagacat attattcctc cttttttcct gatacttttc gattctatac cttcaagtaa
    13381 cttgaaggtc aatagaaaaa agaataaact gaaaaagagg tgagaaaaaa tgaaaaaaat
    13441 gtatacgatt ggccaagtat cggaactatt tgatttgcca aaatcaacga tccgctattg
    13501 ggatgaacaa ggattaatcc gttcaagtag acaagaagag aatgattatc gattgtttga
    13561 tatcgatgat atttttatgc tttatgatat cgttttttat agaaaattag atataccgat
    13621 caaacagatg aagaatttat atggaaaaac attgacggag ttatatgaga cacttgatga
    13681 aacagaaaga agaatccata aagaactggt ggtgatgaaa caaaaacaaa aagagataag
    13741 ggagcaaaaa aaacagttga aattgatgat cgatacaaac gaggaagaat ttcctgtaga
    13801 agagatccct tttgactgta tgatctcaac ggagtttgaa gatattgtgg aaatcaaaaa
    13861 atttctacct aactattcaa gctttgggat gatgagtatg ccagcttcca gttcacagac
    13921 gatgtatgga ttttttatcg accctagtga agtacattta tttactcaag acgtgatttg
    13981 ggagaaaaaa gatacggctg tttatcgtcg attcttattg aaatccgaga tgaaccatgc
    14041 ggaacgtaat aatattttag aaatcagaga aagaatgtat gaaaaaggat ggaaaacagg
    14101 agaagtgatc ggccagtatc tattgacgaa tacggatgaa aacaatatca gaactgaata
    14161 ttatcatgca tggatagaaa tgaaaaaata ggaaatatgt gtttaagata actcgaaaat
    14221 gatggttgtt ttagttataa agaaaaattt tctggttcga ttacgggttt aatgtggggg
    14281 attattagtc gtggatagat agaattttag ttttttagag gaaatatgtc tttaaaaatg
    14341 gaatggttgc aattcttagc gatatatacg atgtaattgt cctcttaaaa aagtatggaa
    14401 aaataattgt attgggtgtt atgtataatt catggtgaac tgagactaat gagaagaaaa
    14461 attcatattg ttatgagtga gattgtatca gatgagaatg ataaattaaa taggtgcata
    14521 aaagcattac acttaaaata cattgttgct tacattatta caatatgtta ataaggcaaa
    14581 aataaataga tggattgaat aaacatattt tttaatcaaa tagtccctac tgtgactttc
    14641 ctcagctatc ggtgatacta ccgatagcat ttttttgctt ataaaataat tataagtatt
    14701 tagagatttt tcttctaaca ttcttcaaac aagaaaaatc acaaaggtta ttgttatttt
    14761 tatatattct ttttaagaat gattgttttt ttatagaaat tttaaaaaat aaaacacgtt
    14821 attgatggat agttagtttg ttatttggta atattataga agttgactgg tagtgattta
    14881 tttttcgtcg ttttatgaaa atgttatttt ttggaaaaat tcacaaatat gtaacgaatg
    14941 tttttctgga atttgctgtg ttaaaaagca gtatgactaa tataaatact attgtcattt
    15001 atgcaatttt ttatggatag taattttaga ggggaatcaa tgaaggaaga atagaaggga
    15061 aaaatagttg caaatacgcc agttagcaaa aatagcaata catcttcaag ctttaaaatt
    15121 cgagagaatc atcttggtag tggaatcaac tacaacaaaa ggattaaacc aattaataga
    15181 tagaattacc aatggaaaaa tagaaaaggt agttattcta tacaaaagtt gtttaattcg
    15241 cttcggttat gaacttagtg aaaccctttg caatatatgg tacagaatat agaaattatt
    15301 gacattacag aaaatgatga agatttaata cagattgtca ctatttgtag atacctttta
    15361 caaggaaacc gagccaataa agctaagaag atgattaagg agttgatgga agacgataca
    15421 agttaagaaa gtgaaactac gcccaaataa agagcaaaaa gagtaattgg acgtggaaaa
    15481 tgcaagaaat gaaccatcga ttcggtggac agtttattag taatgacggt ttgagaacat
    15541 ccatgacgag aatgaaggaa catcctaaat gcgtttagtt gaaccaagtg ccaaataata
    15601 tggccaaata agcgatcagt tatcgatgaa tcccaagtat tctaaactat atattcattt
    15661 tgatgctaag cattgatata tttttttggg attgatgaag gttaaccaat acagggactc
    15721 actgatgaaa ttctagagtc tgaccttggt atcaacgatt tagtgattgt ttctaatgga
    15781 aaagtataaa agtatataag tacattaaca aaataactat tagggttacg ctccagcttg
    15841 tgcaaggcac tggcaggagg aatcgtgaaa tcataattat ggttaagcca cacggacaac
    15901 taaaagtcat acaaagctat cgaatcaatc aacagaagac aatcggcaat cattagcttt
    15961 gtttacagct tacattgcaa cgcttgagtt gaaccgaaaa tacgagggag gtaacgcagc
    16021 cattttgagt tggggaatct ttttagaaaa ttatcgggta ccttgttgtt tagtcaaaat
    16081 tataaggggg agtaatagtg aggaacaaat ttcataaact gttaagtatg tttctagtta
    16141 tgcttattgt gatcaatcaa tttgggaaca taacggttct agctgagtct ttagcagaac
    16201 agccagagcg ttcgctgacg atcgaagttg atcaagagga ggctgctttg ggtgagcagt
    16261 tccagttgcg agtcagagga acaccagacc tgctaaatgc attggtcatt gataagagtg
    16321 aggatttgat tgaaggggaa gtgaagcaac tcaatgatta cgaacgtctg attacttatt
    16381 cttcccagca ttcaggtaaa tacgaattga ccgcaagaag cggtgatatt caatcaaata
    16441 cggtctcaat agaaatcagt gtaccagaag aaagcacaag tgagtctttt aatgtaccag
    16501 aagaaagcac aagtgaatct tttaatatac cagaagaaag cttaagtgaa tcttttaatg
    16561 aagccagatc atctgagcca tctataacgc aggaatctca gattgaaaga caagaggagc
    16621 tcttgaatta tgacatcggc atctctgctg agcgccccac tttgccgagc aggcatacac
    16681 aaacgttaaa gggatacatt caaggaggcg ctggcgatat tcttgaagaa tatggggaga
    16741 tggtcgtacg agttcctaga gcatcgattg gcttaattga gttcattaga gtggaggtcg
    16801 ctgatgattc acctttaaag ccactggcat tttaccaaga aggcacagac gttgtttttc
    16861 catttacctt tgattatgaa ggattaaatg aagccgctca gtcgtataca ttcagtatca
    16921 cgtttgaagt tgctttcttt caatcaggcg gtactgagcc aaataatgac tttcaagtga
    16981 gtcttactta tgctgagcga gcagcaaaca cggaattttc aattacgcct attccttcat
    17041 caagacccat tttttcgaaa ttatactatg gctctagaga aatcgacagc caaggaaatc
    17101 ttgggaatct gctgattgac gggcaagacg tgaccaacag taataaattt attttgcagg
    17161 taaattatga aggatcacga aaagacggtg tagtggttac cgatgtacta ccgcaaggaa
    17221 caactttggc accgaatccg actacatgga gttccactgg aaatagagaa cctatttcag
    17281 gtggggttcg gatcgcagaa gtaacgaatc caggtgaagc aaacgttaga actcattatg
    17341 tttccgagga atttgtggac aaagtatctt ttaataagga aacgaataca cttgttgtgg
    17401 actttggtaa tctagcagag ggtcaatcgt attttattga atatgctctt gaagtgaccg
    17461 atgtcgactt gttcaatgaa ggaagacaag aggatgggtt ttctcggaat cgagcgagta
    17521 tgacctacaa taatcaggta atgagccgag actaccgctt gagaatccct gaaagtaatc
    17581 ttccggcaat ctctttaaga aaaagtgtgg acaaaaatgt tatcaatctc aatgaaaatg
    17641 aactaacgta tactctgcat gtctcgatgt tttctggaac aagaaatacg cttgttattg
    17701 aagatgtttt ggaagaaggc atggaatttg tgtcatttct atcagatatt ccctctttca
    17761 ttgatgccga agtcaataat ggaatagttc gtttcagtgc gaataatacg attggtggtc
    17821 cgacaagttt taccctctct tttaaggtca atgtagaggc gtatgcggtc ggcgatcaaa
    17881 ttttaaactt tgcaacgctt gtgaatagtg acaatcgatt gaatacgagt actgtaagaa
    17941 caaggaaaat cgatgggcgt attaaagtaa ttaaaactga tgagaatggc caaaggctgg
    18001 caggagcaaa atttgaaatt ttatcaagcg attcacaagt gatacaagaa ggtataacgg
    18061 cagaaaacgg agaattttta agtgaaccgc ttcctgtagg tagctatcaa gtccgagaaa
    18121 cagaagcacc ggaagggtat cttttagatg cgacggtaca tgatgttttt ataaatgaat
    18181 actccataga agaagtgacg ttaaccattg aaaacatcct tgatacgggt ggtgttgaac
    18241 taattaaaca tgctgaaggc agtggtgaag tccttcaagg agctgttttt gagttacaga
    18301 accgagaagg agaaacgcta caaaccggt
//
LOCUS       DACEKH010000056        18005 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEKH010000056 DACEKH010000000
VERSION     DACEKH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.057"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..724)
                     /locus_tag="IW662_001741"
     CDS             complement(26..724)
                     /locus_tag="IW662_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ0866564.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(717..2069)
                     /locus_tag="IW662_001742"
     CDS             complement(717..2069)
                     /locus_tag="IW662_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ0866565.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2292..3692)
                     /locus_tag="IW662_001743"
     CDS             complement(2292..3692)
                     /locus_tag="IW662_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ0866566.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3853..4590
                     /locus_tag="IW662_001744"
     CDS             3853..4590
                     /locus_tag="IW662_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ0866567.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4715..5194
                     /locus_tag="IW662_001745"
     CDS             4715..5194
                     /locus_tag="IW662_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ0866568.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5191..5958
                     /locus_tag="IW662_001746"
     CDS             5191..5958
                     /locus_tag="IW662_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866569.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6188..6757
                     /gene="lepB"
                     /locus_tag="IW662_001747"
     CDS             6188..6757
                     /gene="lepB"
                     /locus_tag="IW662_001747"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0866570.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6861..7463)
                     /locus_tag="IW662_001748"
     CDS             complement(6861..7463)
                     /locus_tag="IW662_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0866571.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7569..8038
                     /locus_tag="IW662_001749"
                     /pseudo
     CDS             7569..8038
                     /locus_tag="IW662_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8144..8728
                     /locus_tag="IW662_001750"
     CDS             8144..8728
                     /locus_tag="IW662_001750"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0866572.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8731..9804
                     /gene="prfA"
                     /locus_tag="IW662_001751"
     CDS             8731..9804
                     /gene="prfA"
                     /locus_tag="IW662_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ0866573.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9797..10636
                     /gene="prmC"
                     /locus_tag="IW662_001752"
     CDS             9797..10636
                     /gene="prmC"
                     /locus_tag="IW662_001752"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ0866574.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10629..10808
                     /locus_tag="IW662_001753"
     CDS             10629..10808
                     /locus_tag="IW662_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866575.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10830..11855
                     /locus_tag="IW662_001754"
     CDS             10830..11855
                     /locus_tag="IW662_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ0866576.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12030..13274
                     /locus_tag="IW662_001755"
     CDS             12030..13274
                     /locus_tag="IW662_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ0866577.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13427..14374
                     /locus_tag="IW662_001756"
     CDS             13427..14374
                     /locus_tag="IW662_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0866578.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14508..15137
                     /gene="upp"
                     /locus_tag="IW662_001757"
     CDS             14508..15137
                     /gene="upp"
                     /locus_tag="IW662_001757"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ0866579.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15276..15518)
                     /locus_tag="IW662_001758"
     CDS             complement(15276..15518)
                     /locus_tag="IW662_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866580.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15639..15711
                     /locus_tag="IW662_001759"
     tRNA            15639..15711
                     /locus_tag="IW662_001759"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15672..15674,aa:Thr,seq:cgt)
     gene            complement(15830..16468)
                     /locus_tag="IW662_001760"
     CDS             complement(15830..16468)
                     /locus_tag="IW662_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0866581.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16591..17223)
                     /locus_tag="IW662_001761"
     CDS             complement(16591..17223)
                     /locus_tag="IW662_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0866582.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17574..17873
                     /locus_tag="IW662_001762"
     CDS             17574..17873
                     /locus_tag="IW662_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0866583.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6165 a         3152 c         3558 g         5130 t
ORIGIN      
        1 tgtcacattc ccctttttcg aataactagg ctgtttgtcc ttcaagatag ggcagatcca
       61 attctttacc gtaacgttgt tctaaagcta ggcggatcaa acgagctgcc aggttctcat
      121 ttcttgcaag gataggtcca tggaaataag aaccgaagac atttcggtaa atgacacctt
      181 cagattggtc ttcaccatta tttccttgac cttgtaccac tttccccaaa ggtcgttcac
      241 cttcacctaa gaaagtccga ccattgtggt tttcaaatcc ataataagtt tcattgaatt
      301 cttcgttatg gataacgata tcaccgataa accggttatt ttcctgactt aacgtgtagt
      361 gatccagtgc actgattcct tggatctttt ctccattagc tcccatgtag tagtgaccta
      421 gcaattggta gccaccacaa atggctaaca tgacgccatc attttcgata tagttgatca
      481 aagaatcttt ctttttctgg atgtcttttg aaatgattaa ttgctcaaag tcttgaccac
      541 cgccaaagaa aaccaagtca tatttatcag catcaaaagg ttcatgtata ctaacgatct
      601 ccgtatggca agaaaccccc attttttctg ctatatattt caacatcaaa aggtttccgt
      661 tatcaccata tgtgttcatc aaatttccat aaaggtgggc gatattgagt tcataattag
      721 ccacggttca ttcctccttt gataaatcct tgagcagcta gttctttacg aagctgcaat
      781 acagcagtgt aagtagctaa aatatagaca tgctcagttg gcaattcttt gattttttcg
      841 attacttctg gtaattcttt tgtttctgtc aatttatttt ctggaatccc ggcaactttt
      901 agacgtaaag tcatatctgt atgacggtct cctccagaaa taacagctgg gatatccata
      961 tcggcaaagt tttcaaaatt accatcccaa atccagctga catcgattcc gtctgcgtaa
     1021 ttggcattta ataaagccac taaagaaaat ggataaggag ctagaccaat catatcgatt
     1081 acttgattga tacctacagg atttttgaca agtactaatg tacatttttt cccattgata
     1141 ttgatgactt cttgtcggcc gaatactttc tcgtcatatc ctaaaccttg tttgatttta
     1201 tcaggatcga cattgaagta ctctgccact gcagtagctg ctaaagcatt gtagacatta
     1261 tacataccgc caacttcgat tccgtattca tgaccatcga tcacgaaatc tgctgacaca
     1321 ttgtccatcc gtaccatatc agttaatcga taatccaatt caggacgttt gaagtcacaa
     1381 tttggacagt aatatttccc taagtttgca taagtgatca ttttataatg caagatgtga
     1441 tgacaatttg gacataagac accgtctgta ttgtaatgag ccatttgttc acgatcttct
     1501 tcatggtcaa agccataata ttttctagga ttgactgttt cgatagaatt gaagattggt
     1561 gaatcaccgt tactgataat cgttgcattt ggtgactttg cggcaccgtc tacaatcatt
     1621 ttataggtcg tatagatttc tccataccga tccatttgat cacggaaaat atttgtaaat
     1681 aagaacagtt ctggtttcat atattctgtg actttgctca aacttgcttc atctatttct
     1741 aggacagcaa actttttctg tccttttttt ggttttgcct gcaagaaagt cgatacaatt
     1801 ccctgtacca tattcgcacc agtagggttg gttagaactt catcgaattg ctggcgcaaa
     1861 atattgactg ttaaagcggt agtcaatgtt ttaccgtttg ttccggtgac aacgacgact
     1921 tggtagtctt ttgccaaact atctagtata tgtggatcga ctttcagcgc tagtttacct
     1981 ggtaaacttg atccgccttt aaaaaaagtt tttaatgccc attgcgacgt ttttccaact
     2041 gcaatcgcaa aatgactgcg tattcccatt atgtaacctc caaaaacgat ttgctgacga
     2101 ataaattcaa ctaatcatac catatgttga aaaaaaggtt aaattgcttt tcttattctt
     2161 aagaaaaggt tacggctgaa agtgcatgaa atcggacaaa atcgcatgat ttaacaaaaa
     2221 aacagcttga aacaaaatga ttgtttcaag ctgctgtgat tttattattc cctcttacct
     2281 atattcccat tttaaccgat aataattttc tcgcttggat attcatagcc tagatctcgt
     2341 gtttcttttg gcacgaataa catcaatgta tatagcattc cgatccgccc aataaacatc
     2401 agtgtagcaa tcgtgatttt gcccacaaca gacaagtctc cggtaatccc taaagataaa
     2461 ccagttgttc caaacgcaga agttacttcg atgatgatcg agatcagcgt ttgttcttct
     2521 gtagcagata aaaaaacgat acaaaagaaa cacataccaa gagaaagcat aaagacaacg
     2581 actgattttc gcacatcatc ttgatcgatt ctccgtccaa aaatattgat gttatcttca
     2641 cttttgagga aggaatatag atacaaaccg atgattgcta cagtagttgt tcgaatcccg
     2701 ccgcctacag aactaggact gcatccgata aacatcagta aagaaaaaat gatcagtgtt
     2761 gtgatttgaa aatcacccaa atcgtgaatc tgcagaccag cattacgagt cgtaatcgaa
     2821 taaaacatcg aattgatcca tttgacactc atattggaat cttggaataa atggtccttt
     2881 tccaatagat aaatcaagac agttcccgaa acaaacaaga taacaaaagc taacacagct
     2941 aatttggtaa acaaggaaaa acgaaacgga agcttcgcat tggaacgttt gtataatagc
     3001 cattcccggc actccatcaa tacaggaaaa ccaattcctc cgacaaaaat caaaaacatg
     3061 atcaggatca agaaaaaata gtcatgtgca aagggcttga tggaatcccc agtgacgtca
     3121 aacccagagt tcgtgacagc cgatatcgcc tgataaaaac cgtaaaaaac ggcatcacgc
     3181 caacgatcaa aatagcctct gtaataaaaa taaatagaaa agaatgtccc aaaaagtaat
     3241 tgaaaccaga ttaaaatagc aaaagtgatc cgaatcagtc ggacgatacc agacaaacgc
     3301 ggttgattca tatccgtcat gatcagctgt cgctgtttca gtgtgatccg tcgtttagaa
     3361 aaaatgacaa aagcagtaga aatcatcatg atccccaaac caccaacttg aaataatatt
     3421 tccagcagta taataccgtt atcattgaac acagaattga tatcaaaagt cgatagtcca
     3481 gtcacactca ctgtactgat tgccatgaac agcatgtcca caaaagaaac atggctatct
     3541 ggttcgcgaa aaatcggcat ataaaataga acgagtgata agaccgtcat caatatataa
     3601 taaaaaacga tgatttggat cgatgagaaa tgattggcag caaacttctg tcctcgccgc
     3661 tgcagccttc gaaagaataa atcaacatgc acagtactat cctcctgaca atagtcgttt
     3721 ctaccagtat acaaggggaa ttaggaatac tcaaagaaat tatcaaacag ataacaatac
     3781 agctttcgat taaaatagaa atggcttata ataaggaaaa tcctagaaaa aagaaggagg
     3841 aggaataaca aattgattaa tgctcaacaa atcgtagaaa aaatgaaaaa tcaaaaaata
     3901 aattatgata cagttctgaa aaaaatgatc atgcaatggg agaaagaaga agtaaagcct
     3961 aagatattaa tacatacatg ctgtgctcct tgcagtacgc acacattgga attcatgtct
     4021 caatatgcag agatcacgtt atttttttca aattcgaata ttcatcctaa aagtgagtat
     4081 ttgcgaagat tacatgaaca aaaacgattt gtagaccaat ttaatgagaa aactgaccat
     4141 catgttgcgc taatcgttga tgaataccgt ccaaatgaat tcaccaaaat ggttttaaca
     4201 aaccatcttg aaaacgaaaa agaaggtggt gctcgctgca gtgcatgctt caatatgcga
     4261 ttagatcttg ttgcgcaaaa agcgcaagaa ctaggatacg attactttgg aagtgccctg
     4321 accatctcgc caaagaaaaa tgcagctttg atcaatcaga tcggaatgga tatccaaaag
     4381 atttacggga cacaatactt gccaagtgat ttcaagaaaa acaaaggcta tgaacgctct
     4441 ttgcaaatgt gcaaagagta tgatatttat cgccaatgtt attgtggttg cgtgttcgct
     4501 gccaagcagc aaggatgcga tctgaaagaa atcaatcggg aagcaaaagc ttacttaaaa
     4561 acatcaaccg tagtttttga ggaaaattaa agaaatcttt cggaaaaacg taagaaaaaa
     4621 ataaagaatt tatggtagaa ataaggtaga ctgtataatt tgtccgtagt aaagtaagga
     4681 aacaagaata aatttcaatg aaggaagaga taagatgcga aaagtagcac ctattttgaa
     4741 gccaatcgct gtattaacaa tctcgacgat tgtcgcgact actacaggta ttgtgaatag
     4801 ttccagctac cgtgcaccaa gttcaagttc tagtactgta gaagaaacaa caccaactac
     4861 ttcagtctcg acatctgaac caacaacaac ggaaagtttg aatgaaacca ctacgacaag
     4921 tacatgggac gatacaacaa ctagttcgtc gtatgatgag acatatacaa ctgacagctc
     4981 aagcgaaaca agtaatacgg taccagatgg cactccatcg gattcaacca atccaagtac
     5041 aagtccggat acaagtgcta gctccgaaac gataccaagc agtacgactg tagatgataa
     5101 cacgcaaaat gatgatgcag cacgtacagc acagcaaaac caagattcag gaaataccca
     5161 aacgtataac cagcaagcag gtgattctca atgacatttt tactagacct tatcaacggg
     5221 gtgcataaat tccttggcta tttagacatc agtcctaaat atcttaaccg tggatatacg
     5281 attttaagtg tgattcccac gctttattta ttgagaattg tttatggact ttggcaaaac
     5341 caaaactatc ttcaattttt cttatatgga cttgcatttc tagtgcttgt ttattttact
     5401 gttttaaatg tattttatta ctttttagat aaaaacacga aagccgatgt cactcagctt
     5461 tttgtgaagt atttacctga tgaagcgttc aacatccaga cagaagaatc gatagataat
     5521 ggaaattcga tcgataaagt gaatacacaa gaggtcatgg tttcttatga cgaagattat
     5581 caattgaaat tagccgagaa tatgcggtat ttgattggaa atggtgagat caaaacaaat
     5641 gaactcggtc gcatggatgg atttctggtc gatagaaata cattgtatcc ttattactat
     5701 gtcaagaaaa caggagagaa agagtatgtt ctaaatattg gacgaagcta ctcggacatg
     5761 gaaaaagtag ggaccattcg acttgactca acagaagaca cattggaacc tgtcggattg
     5821 ttcatcgtcg gcggtgattt tgtcaaagac gggttacgct atcatgagcc ttatcgtctg
     5881 aaattgattg caaaaaaggc acagtcgcag gaagaaccag tggtttatag tcgtagccaa
     5941 agaagaagac attcataatg tgatgaaacg ccgtgtatca gtggttgcga taaaaacgtt
     6001 tattcggaat aagggggatg tgacacgtct tttgtcacgt tcctttttta tataacgaga
     6061 aagataagac gaacacatca cttattcttt cctttcattt ataaacattg tagaataaag
     6121 aaagtacaac gaagtattgt gaaaaaacgt aataaataac gaggaaaata caagtaggag
     6181 gtaagctgtg aaagaatttt tgaaaaattg ggggattttg atccttgtat tagcggctat
     6241 tttattagct agagtgtacg tgtttacacc agttactgta aacgggcatt cgatggaccc
     6301 tacattaagt gatggacaac gattgatttc atctaagatc tcaaactatg aacgaatgga
     6361 tattattact acaaaggaac cgggggatga agaacggatg attgttaaac ggatcatcgg
     6421 tatgcctgga gatacagtca agatggaaaa tgaccagttg accattaatg gcaaaaagta
     6481 tgatgaacct tatctggatg agttcaaaaa agaattttca gaagataagt tacaaggaga
     6541 atatgcttat agctcaggat tccaagcaca agcagaaagt tcatccacct ttacgagtga
     6601 ctttgaatat actgtgccaa aaggaaagta tcttgttcta ggcgataacc gcttgatttc
     6661 aaaagacagc cgaatgtttg ggttagtcga caaagatatg atccaaggaa aagttgtttt
     6721 ccgttattgg cctctttcag aaattaaaat catgtgatga aaaaataaaa agaagctgtg
     6781 acatgttttt tgtcacagct tctttttccg aaaattcctt cttatttctt ggagttgctc
     6841 actttttttg caatctcatt ttaagctttt tggctgatgg ataagaaaag cgaagcgttt
     6901 ttgttcataa atagcttttc cccacgatgg aaggtatgac tagtagcaga gacaaagccc
     6961 gttttttcca atcgattatt caattcttct tggctaaagc cattatggac tttcggatga
     7021 ctaatttgtt cattcttgtc aaagtctacg atgatcaatt gaccgccagg agctaaaatt
     7081 tcataaagtt ttgtaagaat attctccgta tcaggaatgt gtaaaagtac aagtgataga
     7141 aggatgagat tagcttggac agcaggaaat tcgacagaag catcagcgtg gatcgttcgg
     7201 acatttttca gatctgctgc ttggatcttt tcttctgcca ttttcagcat agtttctgaa
     7261 gcatcggcaa tgatcagttc tttgaatctt tccgcaagcg gtaaactaac aagcccagtt
     7321 ccgcctccgt aatccagcag gactttttgg tggctgtcgc gcggtagata agtagttagt
     7381 tcttcgcgaa taatcttggc taatgcttga cggtcagggg aatcataatg gttagcgatg
     7441 gaatcaaaaa tattttttgt catcaaatag ctccttacgt aaaactttga aattttaact
     7501 tattatagca cgtgaaaaga aagaaagcat gctatactca attcaaaaag ggagaatcta
     7561 gaaagaatat gccgatatcg tttgtaaaag atagagagga aaaaggaaag tgtgtccgag
     7621 aaatattact agacttgcct gagtggtttg ggcttcctga atcaacggaa aaatatatcg
     7681 aggaatcaag taaactacct ttatggtgtg aaaaaagaaa agaagaatat ttaggcttta
     7741 tcactctttc acagacaagt gaagatactg ccgaaatcta ttctattgta tgggaataaa
     7801 aaaagactat catcaccaag gattaggaac aaaacttttt aaagaagcag aaggttacgc
     7861 agcaaaacat tacaaatatt tgcaagtgaa gactgtagac gaggggcact actctatata
     7921 tgatcaaact atttgtttct acgaatcatt aggattttca aggttggaag tttttccaaa
     7981 tttatgggat gaatggaacc catgtttagt tcttgtcaaa aaattggaac aaaagtgagc
     8041 aatagattta atactaatct gtgcttgatg aaaattctca ctgtaaaaaa tgctgaaaat
     8101 caagtataat accataggaa caaactggaa ggtgaaaaag agcatggcac aactattttt
     8161 taagtatggc gcaatgaata gcggcaagac gatcgagatc ctaaaagtcg cacataatta
     8221 tgaagaacaa aataagccag ttgtcttaat gacaagcggt attgatacaa gagatggttt
     8281 aggcgttgtt tcaagccgaa tcggactgaa acgtaatgcg atgccgatat ttgaagaaac
     8341 agacgtattt gacgtaattc agaaaatgga aagcaaaccc tattgtgtgc tgatcgatga
     8401 agcgcaattc ttaaaaaaga aacatgtcat tgcttttacg agaattgtgg acgaattgga
     8461 tattccggtt atggcatttg gtttgaaaaa tgattttcga aacgagttat ttgaaggatc
     8521 aaaatattta ttgctttatg cagacaaaat cgaagaaatg aaaacaatct gttggttctg
     8581 ccacaaaaaa gcaatcatga atttacatta tattgatgga aaccctgttt atgaaggcga
     8641 tcaagtgcaa attggcggaa atgaagctta ttaccccgtt tgtcgaaaac attactttca
     8701 cccatcaatg attacagaag gagaataagc atgtacgatc aattacaagc aattgaagac
     8761 cgttatgaag agcttggaga acttttaagt gacccagaag tgatttctga tacaaagcgt
     8821 tttatgcagc tttcaaaaga agaagcaaac acgcgcgaaa cagtcgaagt ttatcgtcgt
     8881 tataaagaag tagtacaagg aatcaccgat acagaagaac tgcttggtga aaaactggat
     8941 gatgaaatgg ctgaactagc aaaagaagaa ttatctgaat taaaaaaaga aaaagaagtc
     9001 ttagaagaaa aaatcaagat cttgctttta ccaaaagatc caaatgacga taaaaatatc
     9061 atcatggaaa tccgtggagc agcaggcgga gacgaagcag cattattcgc tggcgatctt
     9121 ttcaatatgt accaaaagta tgctgaagca caaggctgga aaacagaagt gatggaagct
     9181 aacgttaccg gaatcggtgg atataaagaa gtgatcatga tgatcaccgg agacaacgta
     9241 tattcgaaat taaaatatga aagtggtgcg caccgcgtcc agcgtgttcc ttcaactgaa
     9301 tcgcaagggc gtatccatac ttccaccgca acagttgtcg tgatgccaga agctgaagaa
     9361 gtagagatcg atattgaaga caaagatatc cgtacagata tctatcatgc ttcaggtgct
     9421 ggcggacagc acgtcaataa aacagcatct gctgtacgtt tgactcactt accaacaggg
     9481 atcgttgttg ctatgcaaga tgaacgttct caaatcaaga accgtgaaaa agcaatgaaa
     9541 atcttgcgtg ctcgtgtgta cgataagatc cagcaagaag cacaaagcga gtatgatgcc
     9601 aatcgaaaat cagcagtcgg aactggggat cgttcagaac ggatccggac gtataatttc
     9661 ccgcaaaatc gtgtgactga tcatcgtatc ggattaacta tccaaaaatt agatcagatt
     9721 ttagcaggga aattagatga gatcgttgat gcattgatca tgtatgatca aacatcaaaa
     9781 ctggaagcta tgcaaaatgg gtaaaatgac ctatcgagaa gtcctgtcac gggcttcttc
     9841 ttttttagag gatcatggga aagaagcata tagtatccaa tttttatttt tggaacgaaa
     9901 acaatggaaa aaacttgatt ggctccttca tatgaacgaa gagatttcag aagaagaaca
     9961 gcggttgatc gaaaccgatc tgcgacttct gcttgcagat catccacctc agtatttgct
    10021 aggatatgca gatttttatg atcatcggtt aaaggtaact gaagcaacac tgatccctcg
    10081 accagaaaca gaggagttag tcgaatggtg tttagacgag acgcctggtg taccgttaga
    10141 agtgatagat attgggacag ggactggagc aattgctatt agtttgaaag cagcaagaaa
    10201 aaattggcat atatctgctg tggatctttc agaagaagca ttagaagtag cgaaagaaaa
    10261 tgctcagaaa gaaggaacga aaatcagttt ttatcatgga gatacattag gaccagtaat
    10321 ggatcagcaa tttgatgtga ttatttctaa tccgccgtac atcagtcgga atgaatggga
    10381 actgatggat gaaagtgtcc ggtcattcga acctaaaatg gccctttttg ctgagaacga
    10441 tggattagct atttatgaaa aaatagcagt tgaagcacca agcgtactaa aatcaaatgg
    10501 aaagattttt ttagaaatcg gatttcgcca aggtgaagca gtaaaaaaaa tatttcagca
    10561 agcatttcca gataaaaaag tggcgataaa aaaggatctt tttggcaatg aacgaatgat
    10621 ccaagtttat ggttgaagtt gtggatgaaa aattttttat ttgttttttt attataaaaa
    10681 aacacttatt tgacaaggat gtaaacgact ttttggttta tcaacaagtt tatccactat
    10741 tgacaaatag attatccaca aacagtggaa aacttgagga taaactcaga caaaataaga
    10801 atgtatgaaa aaggggttgt ggataaactg tggaaacgaa acgatatgat gaaacagaat
    10861 tgcaagaggc agccgaagca ctgaaagaag gagaacttgt tgcttttcct accgaaacag
    10921 tatacggatt gggcgccaac gctcttttac caaacgctgt caagaaagtg ttttctgtaa
    10981 aagggagacc gcaagataat ccattaatcg tccatgtcgc ctcttttgaa caagtaaaag
    11041 agtatgtgga caactttcat ccggcagcag agaagattgt gaagaacttc tggcctggac
    11101 cattaacttt gatatttaag atcaaaaaag acactttgcc ctcagttgtg acgggaggat
    11161 tatccacagc tgcatttcgt ataccggata acaaaaaaac actagaagta atcgaacttt
    11221 ccggtgttcc gcttgtcggt ccaagtgcaa atacgtcggg aaaaccaagt ccaacgactg
    11281 cagatcatgt atatcatgat ctacaaggaa aaattaccgg gattattgat gatggagcta
    11341 cgcgaattgg tgtagaatcc actgttcttg atttaagtga tccaacagcc atgcctatga
    11401 ttttacgacc aggtgcagta acgaaagaac aaatcgaagc agtcatcgaa tctcctgttg
    11461 ctattgatca gcatcttgta aaagaaaatg aaacaccaaa agcccctggg atgaaataca
    11521 aacactactc acccgataca cgtgttctga tggtcagaga tggcgattgg tcaactgcag
    11581 ttcaatgggc aaaaaataag aagatccgtg caggtgtcat agcaagcccc gaaattgcgg
    11641 atcaagtgcg gacagatact gcagctgttt atatgtataa cgacaactca gtagaagcgg
    11701 cggctaaagg attgtttgca ggtctaagag gattagatga accaacactt ggattagatt
    11761 tgatttttgt acaagtgtat ccagaaactg gattggggaa tgcttacatg aatcgcttga
    11821 aaaaagcagc aggacaaaat tattttgaaa aatgatcaat tggttattgt cgcattcttt
    11881 tagtatgata caatgctatc aagtaaatct tctatcagtg aacgaaaaac taagcgtaac
    11941 taatcgtaat agaagagata cgaatatttg gcaatttgtt tttgatggga aaaataaaat
    12001 tgtaggaaaa taaaaaggag ctgttgtttg tggtagatta caaaacgttt gaccctgatc
    12061 tttgggctgc gatcgccaaa gaagaagaaa gacaagaaca taatttggag ctgattgcct
    12121 ctgaaaactt tgtctccgaa gcagtgatgg ctgcacaagg aagtatcttg acaaataaat
    12181 acgcagaagg ttatccaggg catcgctatt atggtgggtg tgaatttgtc gatatcgttg
    12241 aaaatttagc gattgaccgt gcaaaagaat tattcggagc gaaatttgcg aacgtccagc
    12301 ctcactccgg ctctcaagcc aacacggctg cttatctcgc tttggtagaa ccaggggaca
    12361 cgatcttagg aatggatctt tccgcaggcg gacatctaac acatggatca ccagtcaact
    12421 tcagtggcaa aacctatcac tttgttgctt acggtgttga tccgacaaca gaagttattg
    12481 attataatgt ggtacgtatt ttagctagaa aacatcaacc aaaattgatc gtagcaggtg
    12541 ccagtgctta cggacgaacg atcgactttg cgaaattcag agaaatcgca gatgaagtag
    12601 gcgcaaaact gatggtagat atggcacata tcgcaggatt agtagcagca ggacttcatc
    12661 ctaatccagt tccttatgca gatatcacaa cgactaccac tcataaaaca ttgcgtggcc
    12721 ctcgaggcgg catgattttg acaaacgatg aagcattagc aaaaaaaata aatagtgcgg
    12781 tatttcctgg tattcaaggc ggccctctag agcatgtgat tgcgggtaaa gcagtagcat
    12841 tcaaagaagc attagatcct gcatttaaag aatatagtga acaaatcatc gccaatgcaa
    12901 aagcaatggt caaagttttc aatcaagcaa ttggaaccag agtcatttca ggggcgacag
    12961 ataatcatct gatgttgatc gatgttcgcg agttaggtat aaatggaaaa gaagcagaga
    13021 gcattttgga cagtgtgaat atcactgtaa ataaaaactc gattcctttt gaaacactaa
    13081 gtccattcaa aacaagcggt attcgaatcg gcacgccagc aatcactact cgcgggttca
    13141 aagaagaaga tgctgtaaaa gtggctgaac tagttgtcaa agcattacaa gcaaaagatg
    13201 ataacgctca actagacgaa gtcaaaacag gtgtacgcga attaactgaa aaatttccgt
    13261 tgcataaaaa ataaaaattt aaattccatt agtaaacaga aaagttcaat gttgggcttt
    13321 tctgtttatt ttttgggaaa acggattctt tgacaatatc ggtgatgctc tctagtatat
    13381 gaggtgtaat tttagtcttc acactaaatc tcagggagga aaagtcatga ctgatcgtat
    13441 ctcgttagaa aaggaagcag tagcattcag tgatgcaaat gcgcctcatc cgcgtatcta
    13501 tgaactagca ccggaagaag gaagagtttt actagaagaa gtacaagaat cacctgtgaa
    13561 caaatattcc gtcgatattg aagacttgat ggtggatact ggcgaatggg gaaaactcaa
    13621 tattcgtctc gtacgaccag aagggaatat ggataaatta ccagttattt tttatattca
    13681 tggagcaggc tgggtttttg gcagtgctca aacccatgat aaattgatca gagaacttgc
    13741 ggttcgtacg aattccgtcg ttgtctttcc agaatatagt cgatcaccag aagcaaaata
    13801 tccaacagca attgaacaaa attatgctgt gttgcaacag ctgaataaat tagccaagtc
    13861 aaaaaatctg gatctcgaga atctaatcgt tgcaggagat tcagtaggtg gtaacatggc
    13921 tacagtcatg acgatcttaa caaaagaacg aaagggcctc ccaatatcca agcaattgct
    13981 gtattatcca gtcacaaacg ctgattttga tacagattcc tatcatgaat tcggagaaaa
    14041 ctattttttg acaagagaag gaatgatgtg gttttgggac caatatacga cgaatgaaga
    14101 agagcgtgct gaaatcacag cttcgccttt gagagcaaat ataaatgaac taaatgatct
    14161 tccttcagca atgattttaa cgggagaagc ggatgtatta agagatgaag gtgaggctta
    14221 tgcgcgcaaa cttcgtgatg caggggttcc tgttacacaa gtacgcttcc aaggaatcat
    14281 ccatgatttt gtcatggtaa atgctatgga tcagacaaag gcaacgcgtg cagcaatggc
    14341 attatctact tcttggatca agaaaaaaga ataagctttg cttgtccgca ggaaaacttc
    14401 ttttcaaaaa cggattttct taggaaaaat ggcgagtttg actagtcaat cacttgattt
    14461 tgttttacaa ttaaaaagaa acgacagaac aaaggagagc ttaaaaaatg ggcaaatttc
    14521 aagtgatcga tcatccattg attcaacaca aactaactat tattcgggac aaaaactgcg
    14581 gaacaaaggt ttttcgtgaa gtagtcgatg aaatcgctat gttgatggca tatgaagtat
    14641 cacgggatat gccattggaa gacatcgtga tcgaaacacc gatcactgaa acaacacaaa
    14701 aaacgttgtc tggtaaaaaa gtagcgatta ttccaatctt gcgcgcaggt attggaatgg
    14761 ttgacggtat cttggaattg attccagctg ctaaagtggg acatatcggt ctttatcgag
    14821 atgaagaaac attggaacca catgaatatt tcgtcaaact tccagaagac attgatgcac
    14881 gtcagttgtt tgtagtagat ccaatgttgg caacaggcgg atcagcaatc atggcaatcg
    14941 atgcattgaa agaacgtggc gcgtcgaaca ttaaatttgt ttgtttggta gcagcacctg
    15001 agggagtaaa agctttgcaa gaagcgcatc ctgatattga catctataca gcttctctag
    15061 atgaaaaatt gaatgaacat ggttatatcg ttcctggact aggggatgcg ggtgaccgtt
    15121 tgtttggtac aaaataagta aatacgaaaa agggtgtgac acaacttttg tcacatcctt
    15181 ttttggtcat ttttaacgtt tgatgcagaa cagctttttc acagctttcg tttgcatata
    15241 aaaaaaccat cccaaaaatg cagagatggt caaattcaaa tcttttttag ttctttgtag
    15301 ttaacgatta ctacaccctt actttgcgtc atcaagttcg cattttctgc agtttcatca
    15361 atctcaacaa cagcggaatt tttcaattgc tttgaaatcg ttccagtttg cgacttgccg
    15421 tgaattgtga acattacgtg agtattgatc ttgaattttt caccaatttc cgatggtacg
    15481 atagttgtat caaattttcc aaaagttgcc attttcataa aacctgcctt tcacatcaat
    15541 tataccacgt tttatgaaaa atatcatgaa attaaactaa ttttccatcg gactctttac
    15601 aatcagccaa atagagtata taatttcttt tggataaggc cagcttagct cagttggtag
    15661 agcaacgcac tcgtaacgcg taggtcgtag gttcgatccc tatagctggc attaaaagag
    15721 aagacagata tatcaaggat ttttaatcca ttgatacatc tgtctttttg tttaaaggaa
    15781 ataaagtatg ctttcttagc aatagagcat gtattatttc tataaaaatt taggagaata
    15841 aaacgactag caatatagct gtgaaatcaa atatcacatg aactgcccaa cttgtaatga
    15901 tcgagttgct tttaatggca gcttggttga ataacaatct tgccacccct tgtatcaaaa
    15961 ggatgtgtgc tacagtgtgt aaaatatttc cattatcata agtactgtaa tggataaatc
    16021 caaagatgat tgctgacaag aaactagcga caggaatgct gaacttacta gaaaaaatag
    16081 cgagaaaata cactgagaac aattcttctc caagtatcat gataggcaaa ataataaata
    16141 ataacgggct gaaagcctca tttattggat tgcctgttaa accccatttc aaaatttgtt
    16201 gcaaaaatac cgagactgcg gcactgatta ctacattgat aaaaaaatat ttggctatat
    16261 ttttgacagg agcgacaggt ttcgagaaca tttttttcgt atctttttta ccaaaaacaa
    16321 gtgtaaatgc tataaatcca gtgatagtta aagaaaaaat gctaataatg gatggcgcaa
    16381 atagcaaccc taacaaaccg agtacaacga caatagtgcc caatatccct tttttaagtt
    16441 ctattttttt tcctaaaatg ttattcatga taataactcc tactattcag tttttgaaga
    16501 tagagtcagt atacatgaag agttaaaata ggcaatgttt ttttatggat atttgtattg
    16561 ctaaaaattt atcttatata ttcattttga ttatactact tttatttgta aaaattttct
    16621 agcaaaacca agtgcttgca actgattaag cgtacaattt tttagatgtt ccgagcgtac
    16681 ttcaatcatt tcaaaggagt tgctacttaa atcgatattt tttaaatcag tatcaaaaaa
    16741 gctagcattt tctaaatcat tagtagaaaa cattatttta gtgagggaac agtctacaaa
    16801 atctgcgctt gataagacag agtgctcaaa ggtactttct tttatgtttg aaccagataa
    16861 atttacatag tccattttac aattctcgaa taacgtgttc ccaatataac tttccatcaa
    16921 tttagtacct gttaatttac aattgacaaa tttcacgcga tgcaacatgc tttttgtcaa
    16981 tatacagcct gaaaaatcac agttttcgaa tatacagtcg gtaatctcca ttctttgaaa
    17041 atcattggaa gcaaaaaatg catacttgaa aatagatttg taaataattg gatggcagat
    17101 tatttggttt tcgatgacct ctttttgtga aaggatatta tacagttcaa aatcttcgtt
    17161 ttcatagata tgaaaaaagg atcctttatt caagcttgct tcattaactt taggaaaaat
    17221 catatttaat tacctccatt ttcataaagt catattaaca aaagctaaac caacttacat
    17281 tagcagaaaa tgagataggt tttcgccttt ttcgataacg atagtgtcgc tatctcttga
    17341 aacgttctta gaattgccct gagagacaca aattaggcgt aattctaata aaaatcttag
    17401 aataaaaatt tttactgatt taatttagaa tgtttttaaa ataaaaatcg ttgccataac
    17461 agtgtattga aaggttttaa caatccagta atgatttaaa aaaatctaga tgtagcgtat
    17521 actttgttta tagaatgtag tgggaagaaa tatatttttg ggaggaaatc gtgatgtcta
    17581 aaagtgaaga aatagaaaac atggtactaa aattatatga tctcgtttta aatccagaga
    17641 tcaaagaaaa ggaacgtata cttttgatag acgcaaagac aggcttagaa aaaggtcagt
    17701 attatcctaa agtcataaat aatttggaac gtagtttacg tccgttagca atcaggggag
    17761 aattgtctaa accagtatca ccgttttata tggagatatc aacaatagga aaatttgaaa
    17821 aagaattagg tcgaggaatg gcttcggcac cgattacttt tgggcattta taaaaacgag
    17881 ttattcatct caatgaatta aaaagtatag tcaatctcat aatgcaggct aggttcattt
    17941 atttgaacag agtctgtatt tttttattct aaataaagaa acagatgttt ctttatattg
    18001 tattt
//
LOCUS       DACEKH010000057        17420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEKH010000057 DACEKH010000000
VERSION     DACEKH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.058"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            7..849
                     /locus_tag="IW662_001763"
     CDS             7..849
                     /locus_tag="IW662_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0866584.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            846..1796
                     /locus_tag="IW662_001764"
     CDS             846..1796
                     /locus_tag="IW662_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0866585.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1811..2044
                     /locus_tag="IW662_001765"
     CDS             1811..2044
                     /locus_tag="IW662_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ0866586.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2375..2959
                     /locus_tag="IW662_001766"
     CDS             2375..2959
                     /locus_tag="IW662_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0866587.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3090..3758
                     /locus_tag="IW662_001767"
     CDS             3090..3758
                     /locus_tag="IW662_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0866588.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            3760..5184
                     /locus_tag="IW662_001768"
     CDS             3760..5184
                     /locus_tag="IW662_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0866589.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            5202..5771
                     /locus_tag="IW662_001769"
     CDS             5202..5771
                     /locus_tag="IW662_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ0866590.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5881..7635
                     /locus_tag="IW662_001770"
     CDS             5881..7635
                     /locus_tag="IW662_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866591.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7589..9361
                     /locus_tag="IW662_001771"
     CDS             7589..9361
                     /locus_tag="IW662_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866592.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            9884..10153
                     /gene="rpsN"
                     /locus_tag="IW662_001772"
     CDS             9884..10153
                     /gene="rpsN"
                     /locus_tag="IW662_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0866593.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10168..10347
                     /gene="rpmF"
                     /locus_tag="IW662_001773"
     CDS             10168..10347
                     /gene="rpmF"
                     /locus_tag="IW662_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0866594.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10379..10756
                     /locus_tag="IW662_001774"
     CDS             10379..10756
                     /locus_tag="IW662_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866595.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10777..10926
                     /gene="rpmG"
                     /locus_tag="IW662_001775"
     CDS             10777..10926
                     /gene="rpmG"
                     /locus_tag="IW662_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0866596.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10950..11933
                     /locus_tag="IW662_001776"
     CDS             10950..11933
                     /locus_tag="IW662_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0866597.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11963..12085
                     /locus_tag="IW662_001777"
     CDS             11963..12085
                     /locus_tag="IW662_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0866598.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12103..13626
                     /locus_tag="IW662_001778"
     CDS             12103..13626
                     /locus_tag="IW662_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0866599.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13650..13889
                     /locus_tag="IW662_001779"
     CDS             13650..13889
                     /locus_tag="IW662_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866600.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14082..14543)
                     /locus_tag="IW662_001780"
     CDS             complement(14082..14543)
                     /locus_tag="IW662_001780"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0866601.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14738..14956)
                     /locus_tag="IW662_001781"
     CDS             complement(14738..14956)
                     /locus_tag="IW662_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0866602.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15074..16690
                     /locus_tag="IW662_001782"
     CDS             15074..16690
                     /locus_tag="IW662_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0866603.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16931..>17420)
                     /locus_tag="IW662_001783"
     CDS             complement(16931..>17420)
                     /locus_tag="IW662_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0866604.1"
                     /translation="LLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDDSLKRGY
                     EVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQTEIKRALRVPYSNG
                     PIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKI
                     AV"
BASE COUNT         6039 a         2442 c         3312 g         5627 t
ORIGIN      
        1 aataatatgc ttcaaaattt tattaatggt ctgtatgact ttcacttttt acagaatgct
       61 ctaattacat ctgtagttat agggattgtt tctggaacag taggttgttt tattatttta
      121 agaggaatgt ctcttatggg tgatgctatt tctcatgctg ttttgccagg agtagcaatt
      181 tcttatattt taggtattaa tttttttatt ggtgcgattg tatttggtct attagcttct
      241 atcattatta cttttataaa aagcaatagt gtaataaaag gagatacagc aattggtatt
      301 actttcagtt cttttttagc attaggagtt atccttatag gagttgcaaa tagctcaacg
      361 gatttatttc atatcttatt tggaaatatt ttagctgtac aagatattga taagtggatt
      421 acaattggcg tttctgtttt agtgttggtt acagttgttc tattttttaa agaattatta
      481 atcacttctt ttgatccatt aatggcaaaa gcaatgggga tgaatgtcag tttttatcat
      541 tatctattaa tgattctatt aactttagta gcagtaacag ctatgcaaag tgtgggaact
      601 atattaattg ttgcaatgtt aattacacca gctgctacag catatttata tgcaaacagc
      661 ttaaaaacta tgatatttat ttcggcgaca gtcggagcgt gctcatcatt gctaggtttg
      721 ttcattggtt atagttttaa catagcagca ggatcaagta ttgttttaac agcggcattc
      781 ttatttgtaa ttagttttct aatctcacca aaacaaaaat ttttaagaaa gaaggagaga
      841 aagttatgat gagaaaatgg aaagcagtac tgggaagtct ggggattttg attgctcttt
      901 ttatattcgg ggcatgttca acaaatagta aagacaaaga tacagtggct tcgaacgaaa
      961 aattaaaggt agtagttact aattcgattt tagcagatat tactgaaaat atagcaaaag
     1021 ataaaattga tttacacagt atcgtaccta ttgggaaaga tccccacgaa tatgaacctt
     1081 tgcctgaaga tgttcaaaaa acttcaaaag cagatttgat tttttataac ggtgttaact
     1141 tggaaactgg aggaaatgct tggtttacaa aattagtaaa aaatgcgaac aaagaggaaa
     1201 acaaagacta ttttgcagca agtgatggca tagatgttat ttacttagag ggtcagagtg
     1261 agaaagggaa ggaagatccc catgcttggt taaatttaga aaacggtatt atttacgcta
     1321 aaaatattga aaaacagtta gcggaaaaag atcctgataa taaaaaattc tataaagaaa
     1381 atctagataa gtatattgaa aaattggatt ctctagacaa agaagctaaa tctaaatttg
     1441 cttcaattcc gaatgataaa aaaatgattg ttacaagtga aggatgcttt aaatatttct
     1501 cgaaagcgta taatgtgcct tctgcttaca tttgggaaat caacactgaa gaagaaggaa
     1561 caccagatca aataaaacac ttagttgaaa aattacgcac aacaaaagtt ccctccttat
     1621 tcgtagaaag cagtgtggac gatagaccaa tgaaaacagt atcaaaagat accaatattc
     1681 ctatctattc aacgattttt actgattcaa ttgcagaaaa aggacaagat ggtgatagtt
     1741 actatgcgat gatgaaatgg aacctggata aaattgctga aggcctttcg aaataatttg
     1801 gagggtaggt atggtacaaa ttaaaacgac tactatacta atttatttac tagcaattca
     1861 ggaattatca aaaagaaaaa agaaattaaa aaataatgat ttagcaaaag ttctaagtgt
     1921 tagtccagca tctgtaagtg agatgctggc taaacttaga tatgaggact atttagatat
     1981 aggatttcaa ttaactgata aagggaaaaa atttattgac gattacaaaa aaggttttaa
     2041 gtaaaagact ttatttttag ggaggataag aaaatggcta ttggagtgat tatgatagga
     2101 cttaccctaa tagctggata taattttttt aaatctttct cggattattt tagaaaaggt
     2161 gaatgattta gatgagtcta ttaattcttg taggattgct ttttgtatca gttattttgc
     2221 tctttctcat tagtttagct ctaaggaatg agttcagtaa tagacgttaa cgattgaaac
     2281 ttcaagtcct gtgtaagata atatagttaa ttcatcttat taggaggaag tacccataga
     2341 aaccagagat tatattacaa ctcaccaaaa attaatagaa agtggactaa gccattttga
     2401 aaaagaagga tttcagcaaa ctaagataag gcaactgtgc ttgaccgcag gcgtaactac
     2461 gggtgctttt tataaacatt ttacttctaa ggaagcgtta tttgaagaaa ttattcagcc
     2521 gcatattaat gcgcttatta atatgtataa tgaagagttt accaaatacc tcttaaactt
     2581 agaaaatata gatatagaga aaagttggag agagaacttc aatgaattag atccatttat
     2641 taattatatt tttgagaata aacgtatatt cgatttggtt ttattccaat cagctggaac
     2701 caaatatcaa gatattgttg aaaaaattac atcatttgtg acaaaaaaga cagtctgttg
     2761 tctagataag ttaaagacta tcggaatgat caatcaaaat ttagtcttta atgaagaaga
     2821 aatccacttc tacatttact cattctacgc tacattctat gatatattga agcatagcta
     2881 ttcaaaagaa aagactatgc tattaaccag aagattgtat caattttctg ttcctgggtg
     2941 gattgaatta ctgagttaat taaaaaaaag cttgcattga gaattgttct cagttatgct
     3001 ttttgataag atcgcactgt tatctgaata acggtgcatt tttgtcagct taagataaca
     3061 gtgcgatctt attttttgga ggtgaaagag tgtcaaaaat caatccgtta accttgatta
     3121 ctttaaatat tttttttccg gtcattattt ttttaggaaa gggaaatata tttcaagtgg
     3181 ggtgtttcat tattgctaca gttttagtat tgatgtttcg taaaataaag acatgtattt
     3241 atttcatctt gatttattca gttctgtttt tagtaatgaa aattattgat gctatcaaga
     3301 tagagttttt tactatattt tttgggacat ccttatatat tgtatttcga atgttgccgg
     3361 tcgtaatgat ctcatggttg ttggttagtg tctatactag caatgaatta ttatcctctt
     3421 tagaaaaaat acatttaccg aaaaaaataa tgttaagtat tacagtagtg atcagattct
     3481 ttccgactta tcgttctgaa ataagaatga ttaaagaatc tttaaaaatg agaaacattc
     3541 gcttgtcttt ttttcagcct ctgcaatatt tggaatttct gttagttccc gttcttatac
     3601 gtgcaactat gattgctgaa gaaatgaccg ctaatgcaat aacaaagggg atagaatctc
     3661 ctgtgaaaag aagttcttac tataaaacaa acatgagctt attagactat atcatcctgt
     3721 tttccgttac tatttttttc ttaagtattt tgatttgagg tgaaagaatt gtttgaattt
     3781 aacaatgttt ctttttcata taaggatgtt aaggaacaag cgcttgttga ccttaatttt
     3841 tcaataaaaa aaggcagtct tacattgatt actggttctt ctgggagtgg gaaatccaca
     3901 ctattaaagt taatgaatgg acttattcca agactttatg acggaaaatt ggttggggaa
     3961 attttatttg aaaataactc gctacaatcc ttaacacaag aaagtatatc aaaaaatatt
     4021 ggctacgtaa gtcaaaatcc tagaggacaa ttctttacaa caaatacaac gagtgagtta
     4081 gtattttcga tggaaaactt tggatttaca aaagagcata tggataaaag aatcaatgaa
     4141 ttagctgaat tattagatct ttcaaatata ttagataaaa acattttttc gctatcaagt
     4201 ggtgaacggc agaaaatttc tattggatgt agtttatcgc taaactctaa attaattata
     4261 ttggatgaac cgtcttcaaa tttggacttc aaagcgacta agaaacttgc tgacttactt
     4321 tgtgatttaa aaaataaagg atttactatt gttatagctg aacatcgttt ttactatttg
     4381 aaagaattaa ttgatcaagt tctatttgtt aaaaatagaa ctgtaaaaac tacaactatt
     4441 cagttgttaa aaactgaaaa caataacgca ttaaggaaat ttgacatttt taatagttcg
     4501 attgaagttc cctatatcga aaaggtaagt gcagatgtat taaaagttga agaaattact
     4561 tatgaaaata ttctaaaaga cataaatttt gatgtcaaag ttggagatgt tattggtttg
     4621 gtagggaaaa acggtgtggg gaaaacaacc ttactaagaa tactcatgaa gattattcgc
     4681 cctaccaaag gaaagataat cgaaaataaa aaaaatttgt catcgccgtt tttagtaatg
     4741 caagaaatgg attaccaatt tttcacagaa tcagtgagga gcgagttagc cctggggaat
     4801 gaaatagtta gtaaagatca gcaagaaaaa attttaaaaa aaatggaact ttataaaatg
     4861 aaagatcaat taccgtttga tttatccggt ggagaaaaac aaagattatt ggtttctata
     4921 gcttcattgt caaaaactaa tttatttcta tttgatgaac ctacaagtgg gttggattat
     4981 atcaatatgg aaaggattgc tgaccttatt aaagacttaa aagaaaaggg tgcagtagtt
     5041 atagccactc atgatccgga atttctttat aaaacatgca atagaattat ttatttaaaa
     5101 aatggtggaa taaaaaaaga tataaaactt agagtagaag attcaacttt gatagaacag
     5161 atttttaatg acataacgaa atagaattga ggagaatata tatgaaaaca aaagattata
     5221 tttttattgg tttagctaca gttatttccc tagtaattta ctttttttca ataatggtta
     5281 gtagtattgg tggggcgttt ggtcacagca tatccccagg gatttttgga ttattatctg
     5341 gaatcatatt tgtttatatt agctataact accctagaaa aggaattttt actatttaca
     5401 caattgtttt attgttattt tttacgttaa tgggcggggc gtacttacct tggtttatca
     5461 gttcgattag tatggcgatc ttagctgata ttattctatc tgtatttgga tatgatcgag
     5521 caattcctca agttgtgtct tgggttctaa tgcaactagg cagtgctgcc ggacagtgga
     5581 ttcccatatg gttctttaca gatcgttttc gtcaagactg gatagataga ggacaatctg
     5641 ctgcaacaat ggatgcaatg atccactacg cggtgggtat atggggaatt atttcagtgt
     5701 tagttgtagc aagtttatct atgattggtg tattgattgg aagaaaagtc ttaaaaaaat
     5761 acaaaaaata gaacgtagtt taccttctat ctgtttgtac atctctaatt tacgctatta
     5821 cggaaagcat taacagttgt aaacgaataa gagaagaaat tgaatgcggg ggaggaaaaa
     5881 atgaaagtat acaaaaaatt atttagttat gtccctaaaa tgaaatacta cggatggtta
     5941 gccactttat tttctgattt ttctgtcgta ttaacagtct atggatatta cagtatttac
     6001 aaatttcttc agtctctaat tattgtagga gatgaatatt tagctaagag ttatgcggtt
     6061 caaactgttg cttggttaac tttaggagct ttattttata ttttttctgg cttattttct
     6121 catatccttg ggtttcgctt agagactaat ttgagaaaaa agggaattag cggtttaaca
     6181 gaagcaagtt ttcgtttttt tgatttaaat tcttcgggat atattcgaaa gacaattgat
     6241 gacaacgcgg ctaaaacaca tatggcaatt gctcacttga ttccagatag tgctcaagcc
     6301 atagtaacac ctgttttatc agttgcctta ggctttttca ttaactttaa aattggattg
     6361 gttctaatcg ccatgttagt aattggtgta ttcttgttaa agaaaatgac agagaataca
     6421 aattttattc aaaaatatca agaatcgtta gatgtgttaa gctctgaaac ggtagaatat
     6481 atccgcggaa ttcaagttat aaagatattt ggtgctaaag taacatcatt taaagccttg
     6541 aataaagcaa ttaacgatta tgctaagttt gcatataagt attctttgag tgggaaaaca
     6601 ccttttgttc tttttcaatg gctatttttt ggcatagttt gcattttgat agttcctata
     6661 acctttttta taacttctct taccactccc aagatcttag ctgttgactt gttgatgttg
     6721 ttttttttaa gtggaattat ttttgtttct ttcatgagaa taatgtatgt aagtatgcac
     6781 ttgtttgaag caagatatgc agtcgaaaca ctagactccc tttatttaga aatgaagcaa
     6841 gaccaactaa aatatgggaa cgagacacag attaaaaatt gtgacattgc atttgaaaat
     6901 gtcagctttt catacaaaga agagtgtgtt ttagaaaatt tcaacttagt tttagaacaa
     6961 aaaagaattt atgcattagt aggaaaatca ggaagcggaa agagtacgat agctaaacta
     7021 atttcaggtt tctacaatgt ggataaggga cagattaaaa ttggtggtaa gcctttaaat
     7081 cactattcaa aagacacaat tattaacaca attgcttttg tctttcaaga tacgaaacta
     7141 ttcaatcaga gtatttacga taacgtttct atagcaaatc ctcaagcgcc agcaagtaaa
     7201 gtatttgacg cgctaagatt agccggttgc gaatcgattt tagaaaaatt tcccgaaaga
     7261 gagcatacct taattggtag taaaggagtt tatctgtcag gaggagaaaa acaacgtata
     7321 gctattgcca gagcaatttt aaaagatgca aagattgtta ttatggatga agccagtgca
     7381 tctattgatc cagataatga gtatgaacta caaaaggctt ttcaatattt gatgaaagaa
     7441 aaaacagtta ttatgatagc tcatcgatta tccagcatta aatctgtaga tgaaattctt
     7501 gtcttgtcag aaggaaaaat agtagaacgt ggaaatcata atgaattaat ggctaaacat
     7561 cgagtgtata aacaattggt cactaattat gagaatgcaa atgattggag gcttgacaat
     7621 gaaaaatttt tataaaaaaa aatttgcttt aactgatcag ggcgcagaag ctttaactaa
     7681 agcttctatt tctagttttt ttgtatactg cattaatatg gtaccagcat tcattataat
     7741 gatgctgata gacgaattag ttttagaaaa tgcaaagccc cgttggcttt attttgcagt
     7801 atcgtttgtt actttgcttt tcatgtattg gttgttagat agggagtatg aaaacttata
     7861 taatagtact tataaagaaa gtgcgcattt aagagtgcaa attgcagacg atttgtcaaa
     7921 tttaccatta tcctattttt caaaacataa tttatcagat ttatctcaaa ctatcatgtc
     7981 tgacgttgaa ggtattgagc atgcgatgag tcatgcaata cctaaagccg gcggtatggc
     8041 tctgtttttc ccttttattt cagtgatgct tttggttggt aatgtcaaaa tgggattagc
     8101 tgttattttg ccaacgttat ttagttttgt cttaatcttg ttatcaaaga aatcccaaac
     8161 gaaagccaat actaaatatt acgatacttt gagagaaaac tcggaagaat ttcaagaaac
     8221 tattgaattg cagcaagaga ttaatagctt taatctatct aaaaaagttc aagacagact
     8281 tttcaaaaaa atggaagaga gtgaaaggat tcatttaaag gtagaattaa gtactttttc
     8341 agtcatggcc ttatcctcta ttttctcata tgttagttta gcagtagtca ttctagtagg
     8401 tgttcactta ctgttaacgg gtgaagtgac tatactctac gtcgttggtt acttactagc
     8461 cgcaataaaa ataaaggatt ccttcgattc tatgaaagaa gcagtgcttg aaatatttta
     8521 tttagctcct aaaatacagc gaattagagc tatgaaggaa accagtatcc aagagggatc
     8581 agatagtcct ctgaaatctt ttgacattga gttgcgagat gtctctttct catatgataa
     8641 caatacccca attttagatc atatctcttt tacggctaaa caaggagaag tgactgcgtt
     8701 agtaggtgct agtggttccg ggaaaactag tatcttgaaa ttagtgtcta gactgtatga
     8761 ttatgatgaa ggctgtattt taattgatgg gtatgatatt aaacgtgtat ctccagcttc
     8821 tttgtttagc aaaatcgcta ttgtttttca agaggtaact ttgtttaata caagtatttt
     8881 ggaaaatata cgtattggaa atagtcaagc gtcagatgag gaagtgaaaa aagcagctcg
     8941 tttagctaac tgcgaagact ttattgaaaa actacctgac ggttatcata cattggtcgg
     9001 agaaaacgga agttcacttt ctggaggaga gcgacaacgc ctatccatag cacgggcttt
     9061 tttgaaaaat gcgccaattt taattttaga tgaaattact gccagtttag atgcggaaaa
     9121 tgaaaaacgt attcaggaaa gtttaaatcg tttaatacaa gataagacag tactaattat
     9181 atcgcatcgc ttgaagtcta ttgaaaaggt aaataaaatt gtagtgatgg atcaaggaaa
     9241 actagttgat caaggtactc ataatgagtt gtatcgaagg tcagaaatat acaaaaatct
     9301 cataaagaaa actaaactat ctgagaaatt cgtctatgaa aaagaagctc aatcacgatg
     9361 atgaaatagg ctttaaatta acctacaatt tttgggatat agttataatt ctttttcaaa
     9421 aagaataatt ttattaaaca gcatctaatt ttgaatacac gaaagaatca gtatgattga
     9481 attagttcac ttaatgtcta ttcttataat ataaggaata atcaaagttt taaaaaattg
     9541 gcgataaacc agttaaacat aaaagattga ataaaaagtt atagtttctt tcatattgcc
     9601 gttttaagta gaggaaaagc tacttaaaac ggtatttgtg tttgattgga ctaataaaag
     9661 tgaattctta tttttcagag cctaaactgc atattctttt aaatgatttg attatctcta
     9721 tgtaccgata aattcgtttt caaatagtta tctattcata tgagttttat ttcatgttat
     9781 ttattgttga caagagcaac tcttgttggt aaagtatcag tgtcaaaacg taacgattac
     9841 gatttgaggt gtctaaatca aaataaaagg agtataaagg tatatggcaa aaaagtcgaa
     9901 aattgctaaa gcaaagaaac aaatggcgat gattgaaaag tatgcagata aacgtcagga
     9961 attaaaagcc gcaggagatc gcaccgctct cgctaaattg ccaagagatt caaatccgaa
    10021 tcgtctgagg cttcgtgatc agacggatgg tcgtccaaga gggtatatgc gaaagttcgg
    10081 tatgtcccgt attaagttta gagaacttgc ccatcaaggg ttgattcctg gagtaaaaaa
    10141 agcaagttgg taaaaggagg aaatatcatg gcagtaccag caagaaaaac atctaaggcg
    10201 aagaaacggt taagaagaac tcatcaaact gtagtaaaac cagaaatttc atttgacgaa
    10261 gcgaatggtg attatagacg tagccatcat gtatctttaa aagggtacta taatggaaag
    10321 aaagtaatta aaggagcttc aaaatagttt aatatataac ttttaggagg gaatcaaaat
    10381 ggaaattatt tacccacctt tagttgaaga aggattaaag tattatcttg aaacgaccca
    10441 gcaatcgcta gataaatgca ctttttatcg ttcgatggta gagagaggaa ttattactga
    10501 aacgggttta ccgacccaac aggcaattga aaatggatta gtcaaagatt actatgagga
    10561 ccaaggctta tcatttgatg attttttaag aatctatcca atttttgaag agtatgacga
    10621 ggagttattt caatgtatcg atggttattg ggagatacct gttgatatga aagaaaattt
    10681 agtttcacaa ttggaatctg gcgagttaac ttttgaagat gcacaacaaa ttcaagcata
    10741 tcttgaagat cgataagtat tgaaggagga agatttatgc gtcaaaatat tattttggaa
    10801 tgtgtcgaaa ctggagaacg tctgtatctc accagcaaaa ataaacggaa taaccctgaa
    10861 cggttagaat taaaaaagta ttcgccaaaa ttgcgcagac gggcaatttt taaagaagtg
    10921 aaatagctaa gtggtaaaaa ggtgacaaaa tgggcatacc agttacaatc gtttctgggt
    10981 ttttaggggc tggaaaaaca acgctaatca atcaagcctt gcaagtctca ccctacccca
    11041 gggaagaaat tattataatt gaaaatgagt ttggtgaagt tgggattgat cacaaattat
    11101 tagttcatag cgaggaacgg gtgttacaac taaataatgg atgtatgtgt tgcagtcttc
    11161 gcagtgattt attagcaata ttgatttctt tactggaaac aatagaagaa catcaacaac
    11221 caatttcaca aatcatcatt gaaactacag gtatagcaga tccccaacca atcattcaaa
    11281 cacttttaac agcgcctcaa ctaagaggaa gagtatatat cgatagttta ttgactgtta
    11341 ttgatagtga cagttttgaa cttattgaaa gagaaccaca agcccttaag caacttgtca
    11401 tggcagatcg catttttgtg tctagaaaac aaaacttgaa tttatctgca ctaaaaaaat
    11461 tacagagaga aatcaaaagt attaaccctt tatccgatat tcgaatgttc acacatagtg
    11521 aaacgattca aaaaaaggat ttttataatt tgaaaaaatt taatcatccc ttagtttttg
    11581 aaagtgattc atcagaaaat cactataatg gcaaatacta tcatcaacac catcaagtac
    11641 atgagtttaa atcaattctc ttaacttcag atcaagacat aaaaaaagat tttttactcc
    11701 aatggattga ctggctgatt ttaaccaacc aagggagcct gtatcgtttt aaaggattga
    11761 ttgctttaca agagcaggat tttatagttg ccatgcaaga ggtaaacgaa caggttaatt
    11821 ttcaatacac cactcgacaa aaagaccaaa ccactattat tctaatcgga aaaatgttgg
    11881 atgaagaaag gatcaaacag agttttgata aattactggc ggaaagcaaa taatgataag
    11941 tagtgattgg gggtaatttc taatggagaa aacggatata tctagtgcat atcgacgact
    12001 taaaagcccg aatattaaaa ctagaaaaag ggcattaaaa attatcaaag aacataaacg
    12061 aaataaaatg aagaaattag cataattaag gaaggaaaaa gaatgaagaa aatcagtgta
    12121 ggacttattg ggatagcagc tctaggatta ttgggagctt gctccagcac taatgatgca
    12181 aaagtatcga atgataaaga tggaaaatta gaaattgtaa caacattcta tccaatgtat
    12241 gatttcacca aaaacatcgt cggcgatgaa gcgaacgtgg atttaatggt tccagcggga
    12301 tcagaacctc atgactatga gccctcagct aaggatatgg caaaagctca tgatgctgat
    12361 gtttttgtat atcataacga aaacatggaa tcatgggtac caaaagctaa agaaagttgg
    12421 aagaaagctg ggcctaatgt agtagaagga acaaaagaca tgattttatt gccaggtagt
    12481 gaagaagaag atcatgatca cggagaagaa gatcaccatc atgaactaga tcctcatacg
    12541 tgggtttcac ccaaaatggc aattaaggaa gtttcaaata tcaaggatca gttagttaaa
    12601 ttgtatccga aaaaagcaaa agtatttgag acaaatgctg aaaagtattt gactaaactg
    12661 aaacggttgg atgctgacta taccactagt cttaaagaag cgaaacaaaa aagttttgtg
    12721 acgcaacacg cagcctttgg atatttagct ttggattatg gattgaaaca agttcctatt
    12781 gctggactaa gtcctgaaga agagccatct tctggtcgat tagctgaatt aaaagagtat
    12841 gttaaaaaaa ataagatcaa ttatatttac tttgaaaaga atgctaatga taaaattgca
    12901 aaaactttgg ccaacgaagc tgggataaaa ttggaagtac tgaatcctct tgaaagtctt
    12961 acaaaagaac aaatggataa tggtgaagat tatgtttcag taatggaaga caatctaaaa
    13021 gcgttagaaa aaacaactat ggttgctggt gaagaggtag ttccagaaaa agaagcgaag
    13081 gatgaaaaga cagttgctaa tgggtacttt aaagatgccg atgtgaagga tcgcgaattg
    13141 agtgattata caggcgaatg gcaatctgtt tatccgttgt taaaagatgg aattttggat
    13201 gaagttttcg attacaaagc aaaaatcaat aaagacatga cagctgccga gtacaaagat
    13261 tattatacta ctggctataa gacagatatt gatactatca acattaaaga caacaccatt
    13321 gattttgtag tcaacggtga acatcatcaa tacacataca aatatgttgg atacaaaatt
    13381 ttgaattatg aaaaaggaaa ccgtggtgtt cgttttaatt ttgaaactga tgatgcaggt
    13441 gctggtcgtt ttaaatatat tcaatttagt gatcatggaa ttgcaccaag taaggcagag
    13501 catttccata ttttctttgg tggagaaagt caagaaaaac tctataatga attgcataat
    13561 tggcctactt tctatccagc ttcattgtct aaacatgaaa ttgctcaaga aatgatggct
    13621 cattaaaatt caatttttgg aggaaataca tggtaagaaa agtattattg tctatggatt
    13681 ccaagaatat tgatgcttat cggcgttatc gatttgcaaa gcaaatgaga ttaaatggga
    13741 gtacattagc tgatattgct gcaactttac atgttagtat tgacacagtt catagagtat
    13801 taaaacggga tcctgattgt tgttttatca aggtcaatac tggaagaagt agacgtttag
    13861 caaaacggca taatagcttg gccaattaat tgataatacc agagtagatc atttttccct
    13921 tatttggatt tgatctgctt tttattatgt cttgaattgt caaatcaagc gcaacacttt
    13981 atcaaagaag acttcataag gtgttctcca atctaaacac tttctaggtc tgttattcag
    14041 tttttcaatc atctgattta tcgttgagtt atctaccgta gctatctctt tcgattttgg
    14101 caagtattct cgaatgagtc tattcgtatt ttaatttgtt cattgttgcc aagggcatga
    14161 ggatctggaa aataaaaggg catattattc aatgcttctg taactgcgaa atgattggag
    14221 aattcttttc ctctatctgg agtgatcgtt ttgaccgcct catttggaat actgcttaaa
    14281 agttcaatca ttttctcgct cacataagcg gctttctttc tggggacctt ctttcctgct
    14341 aaaaggaagc atgattttct gtctgtcaaa gtcactaaac aagtaccacc agttttgcca
    14401 gctacaatat cggctttcca atgaccaaaa tcacttctat catctgcgga ttttggacgg
    14461 ccatggatcg aatgagtaag gcaaatcttt ccacgagact cgacatgatt ttttgtatgt
    14521 cttgtttttc ctcgatggtg caatgaacgg atcaggcctt tattgccgtg tgagaggggt
    14581 tctgtatcga aaagatcatt gcaaattgca cgttagatcg tgataaaact aattgaaaag
    14641 tccatatgtt catgcttcaa acgattcgag atttcctctg gcgaccactg aaaatcgaca
    14701 aataaatgac gaacaattga ccaaactcac ggatcattta gaatgtgata acgtccgcat
    14761 ttttttcttc gataagcata atttttttcc gctttggaag gtgaatagtt tttttcttgt
    14821 ttcttctcag ttcatgtaaa atagtaaacg ggctatgctg aatgtcgttg ccaattatac
    14881 taatagaaaa tccttcttcg tgaagcagaa agattctttc tcgttcgagt atagtaagat
    14941 gacgatactg gctcataatt attttccttc taggagtgtt ctcgacaaac ttatactagt
    15001 tggatatcga ttatgactca tttttatgtg ttgcacttaa agtgtaaatt caaggtatag
    15061 aaagaggttc cctatgaaaa aaatacctat tacaatctta actggctatc ttggttcagg
    15121 aaaaacgata cttctaaatc atattctgta cggtgatcac ggaaaggaaa ttgctgtcat
    15181 tgtaaatgac ataggggaag taaatgttga tggggtatta gtcgacaaga aaggttttaa
    15241 aagaactgga gaaaaacttg tttcgatgcc gaatggatgc atttgttgta cattacgcga
    15301 agatttgatt gttgaactag atcaattagt gcaattttct gacttgaatc aaattgttat
    15361 tgaagcttca ggaatcagca aaccaattcc aattattcaa gcaattatat tagctaaaaa
    15421 ccctttagac attgatttga cccaacgatt agagattggt gccattatta tagtagtgga
    15481 tgtaaaacgt ttttgggata atttcgaaac gggtgaaaag attcaggaaa gaaaagctga
    15541 aggtaatgta gttgaagaag aacaggatat tcgtggcttg ttaattgatc aattcgagtg
    15601 ttgtaacata ttacttttaa ataatagtga cttagtgtca accaagacga tttacaaaat
    15661 taacggtcta attgctaagt tacagtccaa tgccaaaata attaggacaa ttaaaggtga
    15721 agtagactta tcgaaaattc taaatacaaa tctttttaat atagatgaga cttcctattc
    15781 agtgggctgg ttcaaagagt tagagcttgg ctatcaaaat catattcctg aaacagaaga
    15841 atatggtatc tcaagtttta tctatcgaaa tcggttactc cttgacgcac aaaaatttgc
    15901 ccatcttacg gataaaaatt ttcctggcaa tattacaaga gcaaaagggt ttctttggtt
    15961 tgcccaacat agtgattttt cgacgatctt tgaaacatcg ggtagctata aaagtattga
    16021 atcattaaat tattgggtag ctagtttacc taaaattgag tgtcaaatga ttatacaaaa
    16081 aaatcccgaa ttgaaggaag cctggcatcc gttgtttggt gaacgcatta ataaactagt
    16141 ttttattggt atgaagatgg atcaacaatg tattaaggaa caacttgagg gttgtttaac
    16201 tgattctctt aatgcagata atttccgaaa aatttcacca tttccatgga aaaagtctaa
    16261 tatgtttagc cagatatcgt taatgaaatt tttaagaaaa gctggtgtca agataggcga
    16321 tgtcataatt attggcattt tgattattac ctcttttatt cccttatttg tattctctat
    16381 agaaaaggag aatgaaacaa ctgaagatgc tatcaaatat gccatcgttc gaattgatgg
    16441 caaggaaatg gatcgcttcg atttggataa gatcaatcat aaactcgtga attatcatcc
    16501 tgcgaaggga caatataata ttgttgagat taaagatgga agaattcgag ttaaagaaga
    16561 taatagtcct gatcaaatcg cggttagaac tggatggatc tcaaaacccg gtcaaacgag
    16621 tatttgttta ccccataagt tagttattag tattgagaaa aaagaaagca cagattacta
    16681 catttactaa tctggaaata gatgaggagg tttttaataa gaaaaagata ttattattaa
    16741 taatagcagg ggctttatta agtgtgttaa gcgcttgtgg acaagaaaaa gaagcggtga
    16801 caacaggcac aagtatcttt aacgggacct gcaatggttt agttaacagt cggttagatt
    16861 acttattttg gctcttcgtc aatgagtgtt ggtgaagaaa ttcaatagaa taatcgagat
    16921 actcacacgg ctagacagct atcttgtcta gccgtgttgt tgttctggct ttagtcagtt
    16981 gggtagtatt cttagttttc ttaaaatatt ttcctcgata taaaaagatt cgttctcggt
    17041 agttttgaaa gtttctatag ccataggcaa ttcgttttag tactttgata tggttattca
    17101 aacattctat agggccgttg gaatagggaa cgcgcaacgc tcgtttgatt tctgtttggt
    17161 atttcttgaa ggtcgtgaac actggttggt agtgctctgg caactccttg tagtcttctt
    17221 ttaggagcgc gacgaattca ggaacatctt gtcttctgat aacggaaaga aaatcctggt
    17281 agacttcgta gcctcgcttt aaggaatcat cataagataa tagccgatcg actatctctg
    17341 cttctgtcag atgatctcga aatgacggac gccagtagcg atgctgacct ttcaattccc
    17401 acgcattctt ttgaagtagt
//
LOCUS       DACEKH010000058        17272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEKH010000058 DACEKH010000000
VERSION     DACEKH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.059"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(176..1462)
                     /locus_tag="IW662_001784"
     CDS             complement(176..1462)
                     /locus_tag="IW662_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0866605.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="IW662_001785"
     CDS             complement(1455..2723)
                     /locus_tag="IW662_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0866606.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="IW662_001786"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="IW662_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ0866607.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="IW662_001787"
     CDS             complement(3495..3941)
                     /locus_tag="IW662_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0866608.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="IW662_001788"
     CDS             complement(4034..4279)
                     /locus_tag="IW662_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866609.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4394..4972)
                     /locus_tag="IW662_001789"
     CDS             complement(4394..4972)
                     /locus_tag="IW662_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0866610.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5049..5579)
                     /locus_tag="IW662_001790"
     CDS             complement(5049..5579)
                     /locus_tag="IW662_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ0866611.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="IW662_001791"
     CDS             complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="IW662_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ0866612.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6436..6831)
                     /locus_tag="IW662_001792"
     CDS             complement(6436..6831)
                     /locus_tag="IW662_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0866613.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6828..8240)
                     /locus_tag="IW662_001793"
     CDS             complement(6828..8240)
                     /locus_tag="IW662_001793"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ0866614.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="IW662_001794"
     CDS             complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="IW662_001794"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ0866615.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(9095..10555)
                     /locus_tag="IW662_001795"
     CDS             complement(9095..10555)
                     /locus_tag="IW662_001795"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ0866616.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10725..11882)
                     /locus_tag="IW662_001796"
     CDS             complement(10725..11882)
                     /locus_tag="IW662_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ0866617.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11916..13286)
                     /gene="radA"
                     /locus_tag="IW662_001797"
     CDS             complement(11916..13286)
                     /gene="radA"
                     /locus_tag="IW662_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ0866618.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13349..13876)
                     /locus_tag="IW662_001798"
     CDS             complement(13349..13876)
                     /locus_tag="IW662_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ0866619.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14106..14912
                     /gene="proB"
                     /locus_tag="IW662_001799"
     CDS             14106..14912
                     /gene="proB"
                     /locus_tag="IW662_001799"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ0866620.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14909..16156
                     /locus_tag="IW662_001800"
     CDS             14909..16156
                     /locus_tag="IW662_001800"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ0866621.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16232..16576
                     /locus_tag="IW662_001801"
     CDS             16232..16576
                     /locus_tag="IW662_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866622.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16693..17019)
                     /locus_tag="IW662_001802"
     CDS             complement(16693..17019)
                     /locus_tag="IW662_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866623.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
BASE COUNT         4701 a         3704 c         3157 g         5710 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
     4381 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
     4441 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
     4501 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
     4561 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aatcgctaaa ggttcttgca
     4621 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
     4681 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
     4741 tgcttataat gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
     4801 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
     4861 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
     4921 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
     4981 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
     5041 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
     5101 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
     5161 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
     5221 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
     5281 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
     5341 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
     5401 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
     5461 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
     5521 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
     5581 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
     5641 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
     5701 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
     5761 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
     5821 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
     5881 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
     5941 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
     6001 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
     6061 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
     6121 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
     6181 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
     6241 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
     6301 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
     6361 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
     6421 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
     6481 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
     6541 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
     6601 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
     6661 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
     6721 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
     6781 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
     6841 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
     6901 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
     6961 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
     7021 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
     7081 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
     7141 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
     7201 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
     7261 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
     7321 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
     7381 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
     7441 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
     7501 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
     7561 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
     7621 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
     7681 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
     7741 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
     7801 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
     7861 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
     7921 ggctcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
     7981 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
     8041 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
     8101 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
     8161 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
     8221 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
     8281 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
     8341 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
     8401 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
     8461 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
     8521 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
     8581 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
     8641 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
     8701 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
     8761 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
     8821 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
     8881 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
     8941 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
     9001 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
     9061 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
     9121 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
     9181 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
     9241 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
     9301 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
     9361 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
     9421 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
     9481 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
     9541 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
     9601 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
     9661 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
     9721 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
     9781 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
     9841 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
     9901 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
     9961 caaaattata agttggcata ccatcgcgtt tttgtatgac aaagtcgcca ccgatattat
    10021 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    10081 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    10141 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    10201 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    10261 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    10321 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    10381 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    10441 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    10501 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    10561 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    10621 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    10681 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    10741 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    10801 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    10861 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    10921 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    10981 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    11041 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    11101 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    11161 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    11221 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    11281 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    11341 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    11401 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    11461 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    11521 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    11581 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    11641 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    11701 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    11761 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    11821 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    11881 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    11941 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    12001 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    12061 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    12121 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    12181 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    12241 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    12301 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    12361 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    12421 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    12481 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    12541 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    12601 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    12661 atcccatttg ttttagcgat tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    12721 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    12781 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    12841 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    12901 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    12961 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    13021 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    13081 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    13141 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    13201 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    13261 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    13321 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    13381 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    13441 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    13501 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    13561 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    13621 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    13681 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    13741 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    13801 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    13861 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    13921 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    13981 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    14041 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    14101 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    14161 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    14221 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    14281 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    14341 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    14401 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    14461 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    14521 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    14581 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    14641 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    14701 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    14761 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    14821 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    14881 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    14941 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    15001 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    15061 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    15121 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    15181 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    15241 cgtgccacta ggcgtgaccg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    15301 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    15361 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    15421 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    15481 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    15541 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    15601 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    15661 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    15721 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    15781 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    15841 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    15901 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    15961 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    16021 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    16081 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    16141 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    16201 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    16261 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    16321 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    16381 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    16441 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    16501 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    16561 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    16621 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    16681 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    16741 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    16801 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    16861 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    16921 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    16981 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    17041 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    17101 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    17161 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    17221 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac gg
//
LOCUS       DACEKH010000059        17208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEKH010000059 DACEKH010000000
VERSION     DACEKH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.060"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(92..1111)
                     /locus_tag="IW662_001803"
     CDS             complement(92..1111)
                     /locus_tag="IW662_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0866624.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1133..2140)
                     /locus_tag="IW662_001804"
     CDS             complement(1133..2140)
                     /locus_tag="IW662_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0866625.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2133..2798)
                     /gene="pgmB"
                     /locus_tag="IW662_001805"
     CDS             complement(2133..2798)
                     /gene="pgmB"
                     /locus_tag="IW662_001805"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0866626.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2791..5085)
                     /locus_tag="IW662_001806"
     CDS             complement(2791..5085)
                     /locus_tag="IW662_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0866627.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5345..7510
                     /locus_tag="IW662_001807"
     CDS             5345..7510
                     /locus_tag="IW662_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0866628.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7814..8647
                     /locus_tag="IW662_001808"
     CDS             7814..8647
                     /locus_tag="IW662_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ0866629.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8749..10449)
                     /locus_tag="IW662_001809"
     CDS             complement(8749..10449)
                     /locus_tag="IW662_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866630.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10956..12116
                     /locus_tag="IW662_001810"
     CDS             10956..12116
                     /locus_tag="IW662_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0866631.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12132..12575
                     /locus_tag="IW662_001811"
     CDS             12132..12575
                     /locus_tag="IW662_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0866632.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13058..13588)
                     /locus_tag="IW662_001812"
     CDS             complement(13058..13588)
                     /locus_tag="IW662_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ0866633.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13693..14244
                     /locus_tag="IW662_001813"
     CDS             13693..14244
                     /locus_tag="IW662_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0866634.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14397..14870)
                     /locus_tag="IW662_001814"
     CDS             complement(14397..14870)
                     /locus_tag="IW662_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0866635.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15008..16480
                     /locus_tag="IW662_001815"
     CDS             15008..16480
                     /locus_tag="IW662_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0866636.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16477..17187
                     /locus_tag="IW662_001816"
     CDS             16477..17187
                     /locus_tag="IW662_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ0866637.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5350 a         3094 c         3537 g         5227 t
ORIGIN      
        1 acagataaac aatgtgttag aagtagtatc ttcttttaca taaaaagctg accataggat
       61 aaatggtcag cgtatttagt gagtcagcta tttagtatgg gttttctaaa ggaacgactg
      121 tctctctttt gatcaagcga tgagggatga ccaaacgtac gccactgctc aattcttcat
      181 ggatcaattc gcgcagcttt tgcatagcga cacgtcctaa ttcggagata tcgatatcga
      241 tacttgtaag gtaagggtgt gttaatgttg agaagatcga gttgttgaag ctaatgacgg
      301 agagtgtctc tggaatgaca tagccataca tttgcgccaa ctgcatcgtg cgaagggcga
      361 agatatcgtc aatgaccacc aatgctgtgg cttcgttttc tttcaacgtt ttctcaaagg
      421 acagataatc ctccggatgc tctagtacta cactggataa cgcaggaaga ccagccatca
      481 tcattgcttt ttgataaccg aaatatcgtt cgaaatacaa actttcatga gtcgtgtttg
      541 tcacaaacag gatattctta tgtccattct ggatcaaaaa ttctgttgct tgtttaccta
      601 gaagctggtt atcattatct acataaacga tttcgttctc atgatgataa ggctgtccga
      661 tcaaagtgaa agggatgtgg ttttggtata aataatcaat gacagtatct tgactatctg
      721 aatacactag aataaaacca tctacttgtt tttgtaaatg catacgctgg acgttctcta
      781 acagcaattc aaaattttta gcagtagcga ttgctgtagt catatcataa ttccgcgctt
      841 cttcgttgat tgcttccatg atctcaagat aaaatgggtt accgatccgc tcttttgaat
      901 caagaggggg taagattacg ccgatggcac tggaaatgcg ttttcctaga ttgcgggcag
      961 tcatgttagg aacataacct agttcatcca taatttttcg gactttcttc tttgttgtct
     1021 cggaaatact aggatgatcg ttgatgacac gagagacggt cgaggtggca acaccggctt
     1081 ttttcgccac atcttttacg gtaatagcca tgataaaaac acctttctct tgttattgtg
     1141 cagagaaccg ataaatcgtc tgactgatcc gttcttgacc aggatggagg ttgatcgatc
     1201 caaatttggg gaaatgaacg atatcaggga tttcttgcgg ttcgattgct aatccgtagt
     1261 ttgaatgcat ctgttttcca ttgacagaga aatcagcttc gaatccagtc gtagaaaata
     1321 ataccatact tttattagaa gttgctatag tcatttggcg accagaatgc ttctcgaata
     1381 attgtaagga agttttggaa agcctaggat gatgcaaaag aaatacatca tctagtccat
     1441 ctggataaca tgcaaggatc tcttgcagtg ttggggattt tctaaagtcg attggcagtt
     1501 tttctttttt tgtccaattt ccagtaggca gtttttcttg atctaattca agcacaccat
     1561 ctaaaaagac cgttaactga tgttcataga tgtctcttgt tccatttcct gaaaggttga
     1621 agtaggcatg gttggtcgga ttgcagatgg tttcaacaag gctactagca gaggttgtca
     1681 tctctaattc gttttttgtt aaacggtaag tgatcgtagc agtgattggt cctggatagg
     1741 tagaaaattc atcttttaag taaaagacca cacctattga tgatggagtt ttgaaaactt
     1801 ctacttccca aaattggaaa gaccagcctt tcgtgccacc gtggatatgg tgtgatccgc
     1861 agtttttctc caatctatgc tcaccccaag aagcatcccg gatccttccg gctacaggtc
     1921 cgacagttgc tccaaaaaag cttttgtctg ttagaacatc ttcgaaacga tcgtaagaca
     1981 ataaaacatt ttctgatttt ccttctttgt ctggtgcgaa gatttggtgc aggcgggcac
     2041 cgtaattgag aagaacgact tctaagtagt cgttcttcaa tgtgattttc tttatttcag
     2101 tctctggaac tggtacgacg gagatctctg gattatccat ggtcatgcca cttttctttc
     2161 aagtaatcga tcgtaagaaa agtcgtatca gggacaagcg ggatctcctc tccaaggtct
     2221 tcagcacgtc ccacgccaat tggttgagcg ccacttgaga ggatggcttg gataccggct
     2281 tgcgcgtctt cgatcccgat acattcttct gcttttagtc cgacagcttt tgctgcaagg
     2341 aggaagatat ctggcgcagg ttttccgttt gttgcttctg ccgggtcggc aatagcatca
     2401 aagtaaggtg taagacccat tttttcaagt aggaatggcc cgtttttact tgcagaagca
     2461 agagcaatct tgattttttc ttcttttaaa gaatccagta aaggcacgac cccggggaag
     2521 acatcttttg gttcgatcgt ctggatcatc tctaaataat aatcgttttt tcttttcgct
     2581 aattctgcaa attcttcctt tgaaaaagag tgttcttttc cgccataagc taaaagcaaa
     2641 gagagtgagt cttctcggct gacgcctttt agctgttcgt tgaatgtgtg gtcgatcgta
     2701 atgccgattt cctgaccaag ctttcgccaa gcacgataat ggaattcagc agtatcggta
     2761 atgacaccgt ctaaatcaaa caatacccct ttaaacattt gcttcctcct ttcttagcgt
     2821 tatttctaga cgatccgcta aagaataaga ctgttcatag acgtaaattt ctagaggaga
     2881 accttctaat aactcgaaag taacttgttc atctgtgact gagatgaaca ataaacgtcc
     2941 gcgataattg atatggaaag aataagcttt ccaagttttt ggcagaaatg gtgcgaatct
     3001 caactgatca tgggcagttt tcatttgagc aaatccttgg acaatcgaca accagctacc
     3061 agtcatggaa gtgatgtgca agccgtcttc tgtgtcgtta ttgtaattat ccaaatccaa
     3121 gcgagctgtt cgctggtaca tttctacagc tttgctttcc attcctaatt ctgctgctaa
     3181 aatcgaatgg atacttggcg aaagggaaga ctcatggaca gtcattggtt catagaaatc
     3241 aaagtttttt tgcttttctt cttttgtaaa agtatcattg aagaaataaa tcccctgtag
     3301 tacatctgcc tgcttgatga agcaagatct aaggattttg tcccaagacc aatgctgatt
     3361 cagaggaaga tcgcttgtgg gaatatcgga tacaggcatg agatctttat ctaagaatgt
     3421 gtcatgctgg acaaagattc ctaattcttc gtcaactggg aagtacatgt tctctgcgat
     3481 atctttccat ttggctagtt cttcttcgct gatagtgata gcagcttctt tttgataagt
     3541 taaataattt tctcgcgtgt aacgcaagac ccaagctgcg atcgtatttg tataccaatt
     3601 gttattgata ttgttctcat attcgtttgg gccggtcaca ccgtggatca tgtatttatt
     3661 tgctcgtttt gaaaaatgga cacggtctgc ccagaagcgt gcgatttctg ctaaaacttc
     3721 cagtccttca tttttcaaat attccgtatc gccggtgtaa ttcgtataat tgtagattgc
     3781 ataggcaatc gcgccatttc ggtgaatctc ctcgaatgtg atttcccact cgttatggca
     3841 ttcgacacca gtgaaggtga ccataggata aagcgcgcct tttaatcctt gttgctgagc
     3901 gttgtggatt gcttgcggca gttgattatg ccgatatttc agcagattct tagttacttc
     3961 aggtttggct aaagcaagat aaagagggac agcatacgct tctgtatccc agtaagtggc
     4021 tccgccgtat ttttctcctg tgaatccttt ggggccgata ttcaatcgtt catcttcgcc
     4081 gtagtaagta gagaatagtt ggaacaaatt gaatcgaata ccttgttgtg cttcttcgtc
     4141 tccttcgatg accacatcgg caagcttcca tcgttctgcc caagccgctg tttgctcttc
     4201 ttttgcttgg atataattgt ttgtctggtt cgttaattct gagacagctt tttctgcttg
     4261 ctgcgcttct ggaatatctc tactagtaag aacgataatt tctttttcca ctgaccatgt
     4321 ctcaccttga gataattctg cagacaagca accatgtaat gctaaagtat cttcgtgata
     4381 gtcagggatg actgattttc cattaacgaa atggcgcatt gctgcagtaa cagtaaattg
     4441 ttcaatccca aagttgtttg gaatagtttt agtcgtcaaa tagccgattt cttctatagt
     4501 tccacgatca atctcattcc aaaaatgttc atcatagttg ctgtcttcgt tttgaacatt
     4561 tccatccaat tttgatacga gtttgactgt tccgctacct tcaagcattt ctatagttaa
     4621 atagatataa gctgattctt ttttgacaat gctcagaaaa cgttcgaagg aaatgcgtac
     4681 tttattagta gaagtagtga ttgtgaatgt acgagataat acaccatttt tcatatctaa
     4741 ttcccagtaa aagtcttctg gttccaaaga agccaagtcg atcagctgat cgttgatcga
     4801 aatatctatc gcgataaagt tcaaggcgtt gatcactttt ccaaagtatt ctggataacc
     4861 atttttccac cagccgacac gtgtcttatc gggataccag acacccgcca aataagtacc
     4921 ttgatggtgg tcccctgagt aagtttcttc aaagttcccg cgcatcccca tataaccgtt
     4981 tccaatactt gttaaagatt cttgcagtcg aagatttttt ttatctaatt gtgtcgtgct
     5041 gattttccat ggatcaatag caaatagtcg tttgatctgt ttcatttttc tcctcctaat
     5101 gagctgtttt atacgttcat aataaacgca accgattgca caagtcaata gacaaaagaa
     5161 aatatttcat agaaaaaagc ttaaaaaaat aaatttttag gagaaaatta tagaagggaa
     5221 cacacgtatt ataggtttta tagaatggta tagacagaaa aataaaaaat aaagcattta
     5281 caaaaaagaa aacgcacgct ataatacgtt taacgaaacc gattgcgtaa aggagctaaa
     5341 cggaatgaaa aagatattta gttttgagtt ttggcaaaaa tttggaaagg ctttaatggt
     5401 tgtggtagca gttatgccgg cagctgggtt gatgatcagt atagggaaat caattccctt
     5461 gatcaatcct gattggacgc cgcttgtcac aacgggcgga gtaattgaaa atattggttg
     5521 ggcaattatt ggtaatcttc atattttatt tgctttagca ataggaggaa gttgggcaaa
     5581 agaaagagca ggcggagcat ttgcagctgg gatctcattt attttaatca atcggatcac
     5641 tggggctatc tttggtgtca caagtgacat gcttgcgaat gaagatgcat ttacccacac
     5701 tctgttcggt acgaagatca tggttaaagg attttttaca agtgtattgg aagcaccagc
     5761 tttgaatatg ggagtattcg tagggatcat cgctggattt gtcggggcaa tggcttataa
     5821 taagtactat aattatagaa aattgccgga tgctttgtca tttttcaatg gaaagcgctt
     5881 tgttccgttt gttgttatct tatggtcaac aatcgtggca ttgattcttg ctgtggtgtg
     5941 gccaaacgta caggctggaa tcaataattt tggtttatgg attgcagaat cacaagaaag
     6001 cgcaccgatt ctagcaccgt ttttatttgg aacattagaa cgtttactat tgccatttgg
     6061 attgcatcat atgctgacga ttcctatcaa ctatacacag cttggcggat cgtatgagat
     6121 tttatcaggc gcacaggcgg ggacacaagt atttgggcaa gatccgttat ggttggcttg
     6181 ggccactgat ttagtgaatc ttaaaggtgc aggggatatg tcgcaatatg agtttgtgtt
     6241 gcataactgg acgccggctc gtttcaaagt aggacaaatg attggttctt caggtatctt
     6301 gatgggaatg gcttttgcga tgtatcgtaa tgtcgatcct gataagaaag caagatataa
     6361 atcaatgtat ttttcagcag cgttggctgt ctttctaaca ggtgtaacag aaccattgga
     6421 gttcatgttt atgtttgctg ctgtgccatt atatgtcatt tattcagtga tccaaggggc
     6481 tgcatttgcg atggcagata tcttgccatt gcgggtccat tcatttggga atatcgaatt
     6541 gctgactcgg acaccgttag ctatcaaagc gggtctaggt ggagatctta tcaacttcgt
     6601 gttgtgtgtg atcgcttttg gtgttgttac gtatttctta gcaaatttct tgatcaaaaa
     6661 attcaacttt gctacaccgg gacgtaacgg aaattacgac aatgacagtg aagaaaccgt
     6721 tgtatctaac agcggaacag gaacagcaga tcagcaagtg gtccagatca ttcatttgct
     6781 gggaggaaaa gaaaatatca aagatgtcga tgcttgtatg actcggttac gcgtaagtgt
     6841 aaatgatcga gaaaaagtag ggactgaaga agcctggaaa cgagcagggg cgatgggatt
     6901 gatcgtcaaa gacaatggag tccaagcagt ttatggacca aaagcagatg tcttgaaatc
     6961 agatatcgaa gatctgctgc aatcaggagc agagattcca gagccagaaa tggtttctac
     7021 aggaaatgga caagcaggta gtaagcaata cttaggtata gaacaagaac tagtttccgc
     7081 agcttctgga gaagtgattc ctttatctga agtaaaagat ccggtattct cacaaaaaat
     7141 gatgggtgac ggctttgctg ttattccaac atcaagagaa gttgttgcgc cgatcgctgg
     7201 aaaagtgaca agtattttcc ctagcaaaca tgcaattggg ctggaaacga aagatggtat
     7261 cgaagttttg atccatatgg gaatcgatac agtccaaatg aatcagccag cttttgagat
     7321 tgctgtcaaa gaaggccaag aagtaggaac aggaacgaaa ttagctgaga tggatcttga
     7381 tgtgatccaa aacgaagaaa aagatccaac gatcatgatc gtatttacaa acgataaagt
     7441 agaagaagta gtgatcaagc aattaggtac agtgacagct ggtaaagtga tcggctccat
     7501 taaaatgtaa ggagaggttg tgaaaaagct gtcctgcatc aagtcataag aacagccaaa
     7561 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgagaag cagcagtttg
     7621 aatactgttt agtggagagg tcgtgaaaaa gctgccctgc accaagtgat aaaaacagcc
     7681 aaacaaaaaa tagctcctgg tccattgagc ttattactga ggagttagca ggagaacact
     7741 atttgctgga gaggttgtga caaaaatttt gtcacaaccc ttttgtttcg aaatgaagac
     7801 aaggagaaaa ccaatgaaat tcatgacact caacacgcat agctggcttg agccagaacc
     7861 agagaaaaaa ttgcaagaat tagcagataa gatcctgtta gaagattatg aagtgattgc
     7921 tttacaagaa atcaaccaat tactggaatc tgaagaagta gaacctgcga agctaatgaa
     7981 gttctgtccg gtaaagaatc aagtacctat ccgtaaagat aattttgcct accgtttagt
     8041 tcagctttta aaggaacacg gaaaagagta tttttggagt tgggaaatga gccacatcgg
     8101 ttatgataaa tatgaagaag gaaatgcact gcttacgaaa aaaacattgg aaagtcaggt
     8161 ccttaccgtc tcccaatttc aaaagaaaga agattatcag acacgcaaga tcttgattgg
     8221 gaaaacaaag atagatgatc aggacatttt tgtctcgagc tgccactttt cttggtggac
     8281 agataaaaaa agtggctttt attttgagtg gaaaaacttg gaaaactact ttttggaaac
     8341 tcgcgtacca ctttttttcc taggtgattt caataatcca gtagactctc aaggttatta
     8401 tactgtaaga gaaagttgcc ttcttcttca agattcgtat gttgtcgcga atgaaaaagg
     8461 aaaagcagct actgtggaaa agaaaataga tggttgggaa caaaatacag aaaaattgcg
     8521 tatagatttt attttcgtcc ctgaaggaat gcaggttaaa aaatatcaac ggatttttga
     8581 tgggatcgat agtccaatca tcagtgatca ctacggggtg gaaatcgaat tagatgtgaa
     8641 cgaataaaaa atataacata taacataaaa aaactgatta aaataaaact ctccaaacga
     8701 ttataccgtt tggagagttt tttgatcgga tgtatttatc ccttttgcct aagcacgtaa
     8761 aagcttgtat ctatgataaa taaataaaca tagcaaggta aaccagccaa tcaaagtaag
     8821 agaaagaatg aaatagaaat agattggcgt ttgataatga agcgtgagct cgttgattcc
     8881 tttttgctgc ttcacgattg gggtcccgat atctgttact tcataatcag tcaatgtttt
     8941 tccattttgc tgtagcacag tccgatcgta gacgataaca gggatatgaa cagttttgtc
     9001 cccagtgttt ttccacttga ttgtcaaaag accgcctgga tgtgttttta taaaagttgt
     9061 ttcgttttct agtatttctt cgttataccg gtcgtattta ttgtcttttg tctccttata
     9121 gattggcaga tagtcaggtg tggttttttg gaaaacgaac aagctggtag acatatcttt
     9181 gctgaagaat gttttccttg cttcttccgg cgtatcgaat agataagtat gtcgactgac
     9241 aaatgtttca ttttcccact tttccagatg ctggtataag ttctggatcg tctgtgctga
     9301 aaaaataaga atgccaaata tacttaaaag acggaaccac ttttgttcag accactggtt
     9361 cagtgaaagg gcaaggtaaa gtagaagcaa tagcgtaaat ggcacgaaaa atcgaaatgg
     9421 aaactggatc aaactaataa aaggcacatt tttttcgatc aatgtcgtcc atggaatcaa
     9481 gctagtagaa agaaaaaaga aaacaagact tgtgtacaac acgatttttt ggaataaggt
     9541 gttcttttgt tgaaagaaaa taaaaagcaa aaaacctgat aatatcaata taggaaaggc
     9601 cttagggtaa tggagccaat aactgccatc cagatctatc gccttttgac tcatttctct
     9661 gttaacaaag ggaggaagaa tcttattccc actgtaaaca atagctaatc ctgcccaaat
     9721 atttgctgtc agaacaaaat aaagcaagac cgacttaatc agttgaagca gtgcttttcc
     9781 tttttcagtc tggttaaaaa agtaataaag ataaaaaggg atatacatca aagctaacat
     9841 gatcgaagac agtaaatgga tctgtgtcat aagtcccact gagacagcga ctttgataac
     9901 agggaacttt ttatgaagga cgaaatcagc aatagggatc atacataaag gataaaaaca
     9961 agtcccccag ctagtgaatc cttggttgat agaccagtat tgaatagaat acgtactcat
    10021 gaataaaacg gctactggta cacttaccca atttcgaaca ttgacatatc tcagtaaaat
    10081 cagcatagaa aatcctgaaa taagataaag aatgaaattt gaaagaatct gataatgaaa
    10141 ccagctacct gcaagcaaca cgatcaatcc ttgtaaataa gcgaaaaatg ggccgtaaac
    10201 ggcattcacg atcctgccac tttgctggaa tccataaagg gaaggaaaat aatggaaatc
    10261 cccatattta atttgttgcg cggcatcata gaaccgattg taatggaatg gggcatcagc
    10321 acccaaaatt agtttatgtg tgaataaatg aggcatgaga aatgccaaag caatgaaaaa
    10381 aattgctaaa aatgggccat attttttcaa ttgtcgaact cctttgcaaa ctttagataa
    10441 attcatcata tatcctatgc gagaggtagg caagaaatta gaaaattcat gaaaatattt
    10501 atgaatttta caagaaaaac ataagtgaaa gttctaagga gagaggatgc gacataagtt
    10561 ttgttgcatc tccttgtttt gaaacagaat ttttcttaat actcgaaaca aagtagcttt
    10621 ttcatgacct ctcggtaaac ggtgttcaat cagctgcttc tcggtaataa gctcactagc
    10681 ctagaaaata tgagaagcta ttttctgact agcgctctta ttactcaaag cataacgcag
    10741 ttttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccaa
    10801 cccaacaaaa aatatgagta gctatttttt tgggctgtac ttattacttg atgcaggaca
    10861 gctttttcac aacctcatcg gctttcattt tctatttttt tcctgaaccc atataataaa
    10921 agaaagaaca aaaaaacttg caagggagga cacatgtgaa gcagagtaag caaaaacagt
    10981 ctcgtcgact tttgtttcgt ttgtttttat taatatgtat cgtcgtaggg ttactgatcg
    11041 ctttatatcc attttatgtg gattctttaa atagttttat tgatcaaaaa cgtatcgaac
    11101 aagtacaaaa acggaccgca gctgaaattg aagtgcagcg aaagaaaatg gaggagaaaa
    11161 accaacgatt aactgaccaa ggattcaatc ctggtactga tcctttcaat gaaaagaaca
    11221 gaaatgaaag tacaacatcc agccaattag aggaatggct gatcggaagt atcaatatac
    11281 ctaagatcca attaaatatt tcactttatg atcggctcaa tggtaggatc cttgaaaatg
    11341 gagcaggcgt cttgcaagga acctctttcc cgcttggtgg aaattcgacg cattcagtga
    11401 tctctgctca cagcggttta cctaatcgac ggttatttac cgaattagat cgtttagaaa
    11461 acggagacac gtttattttg actgttttgg gggagaaatt ggcttatcaa gttaaaaata
    11521 tacaagtggt actgccagat gacacgtctg ttctaacgat ttcagaagga gaagacttag
    11581 ttaccttgct tacatgtacg ccgtatatga tcaacacgca tcgtttgcta gtgacaggac
    11641 atcggattcc ttataacgaa tcggtaaaaa aagaagaagg aaaaggaaat caggaaagaa
    11701 ccatgcggca atggtttatt cttgcaggaa cgataatagc tgtagtcata ctgttagtgt
    11761 ttatcggacg actaatctat cagtatagat taagtaaaaa agtgcttgat ttttcattta
    11821 ttattagtga ttcgtcaggg aaaccggtaa atggtagttc attcattttg aaacataaga
    11881 aaaaaacact tactagaaat ggtgtgccgt tttcagtaca aagcgaccat catgggaaag
    11941 tcaagctgga tcaattgcct ggtggaactt atcgaatcgt ttcagctgat gatcctaaag
    12001 ttgctgtttc ttttggtatt agaaaagtaa aacaagagaa gatatacttt tttgaggggc
    12061 gaaaacttgt aaaagagctt caaaaaaatg gtttttattt taaaatcaat gaataagtaa
    12121 ggagagaaaa aatgatccgc acaacaacaa aattgtctgc caaagaactg gaatctgttc
    12181 tatcgatttg gctgcatgct aacttggatt ctcatgaatt cattccatca acttattggg
    12241 agaatcaagt agaatctgtt aggaaagctt taccagatgc agtgatttac ttagcagaaa
    12301 tagatcaaga aattattggt tttgctgggg tgattgatca gtttatcgca ggaatctttg
    12361 tgaaaaggga ataccggcaa caaagaatcg gtacgcaact aatgaaaaaa ataaaagaaa
    12421 ccaataaaac actgactttg acagtctatc aaaaaaatca atcagctatt catttctatc
    12481 tttcacaagg ttttacaatt caaacacagc aaaaagatga agcagtaaat gaggaggaac
    12541 ttgtgatgaa atggaatcaa aacgctgatt tttgatgaac agaataacga aataatgctc
    12601 ccagcctaat tttttgctgg gagcattatt tcattttgaa gtcactaatt taactgtgtg
    12661 aatatgcagt tgttacaatc tttgataaac ggtgttcaat cagctgaccc tcggtattag
    12721 ctcaaaaaac ggcaaaatat gaggaactat ttttccattt gtttctctaa tactcaggtc
    12781 aaaacgctga tttcacaacc tcttgataaa cggtgttcaa acggctgctt ctcggtaata
    12841 agcccactag cctagaaaat atgaaaagct attttctaac tagcgttctt attactcaaa
    12901 gcataacgct gatttcacaa cctcttgata aacggtgttc aatcagctga ccctcggtat
    12961 tagctcaaaa aacggcaaaa catgaggaac tatttttccg tttctttctc taatactcag
    13021 gtcagaacgc tgatttcaca acctctcttt cacaacctta ttttactcgt ttataatttt
    13081 catcgaagta ttttccgcta cggatatcat ctggcattcc atcgtatggt tcttcgctaa
    13141 taacatcttc attgtctata cctgcatcac ccatataagt gatcgtagca aattcaggaa
    13201 caagtaattt tggataagtt tcatattttt cacgagctgc ttccatttct gcgatatgtt
    13261 cgttttggaa agtaacagtg atttctggat gttcttcaac ccatttaggg aaaaagatca
    13321 ttccgtgaag tttcttttca tttggcataa agtccaatgc cacgtctgtc cctgttggct
    13381 ctgtccaagt gattttgaat acgccgtccg tcaatttaac gatatcagct tcttggtctt
    13441 ttacccaacg gcctttgacc attccgccat ggatacggta atcaacagta tggtcatttt
    13501 tggcatacca ttcatattcc caaccattgt catacgtata gataaagtga gttccaataa
    13561 aatcttccaa ttctttaaat tctttcatgt ttaagcatct ccttataaat taaaacatgt
    13621 aataaactac atgttttcga ttactcattt atgatagaat ggatttaaag atttgtcaag
    13681 gagggaaaac tgatggcaag aaaaaataca cttcgatata ttttgttagg gttattaagt
    13741 aagaagaaga tgacaggata tgaattgaat caatcgttca aaaatgaaat aggagaattt
    13801 tggcaagcaa aacatagtca gatttatcct gaacttgcaa aaatggaaga gcaaggaatc
    13861 attcagcatc aagtagagat cacaggtgaa aaattggaaa agaaagttta tgaattaaca
    13921 gaagaaggga aggaacaatt ggcagagtgg attcatacac ccaccaatga actaccggtt
    13981 aatcgagacg aatttgttct gaaattgtat ttcgtcaaag acgtgaatga tccggcatta
    14041 gtagaaatta ttcagcagca gcgtgacctt catgaagaaa aacgacttca tctattagag
    14101 cgaaaaaaaa cgatctttcc tactgaaaaa gaaaaacatg aaaattacgg acactacctg
    14161 attttagcgc atgcgatcaa tcgggaaaca gaatatgcaa attggctaac agatgtatta
    14221 gaagaaagta accgaaaaaa ataaaaaaat atccgaacag tgatggagaa ttcctttcta
    14281 tatgaaagaa acgcagatca ccgttcggat attcttatgt tcaaacaggc cttttttcaa
    14341 gtatttatgt agcatctact tattaaaaag cttggtactt aggagattag tggcgtttag
    14401 agttctttta acaactgttc gtagtcagct ttttcatcga caggaaggag cttgtaagtt
    14461 aattcgtctg tctcgttgtc atgaatgatg tacatcttag cattctcttc tttgaagtta
    14521 ccgatttgta aaatcgtata gcgttcgtcg atcaattcga tatctttgat tgcttttgaa
    14581 aaaactttac ggacatggtg cattttcttt tggcggattt ctgccttttc tgcagctttt
    14641 gacaaagtaa atccgtcatc taaatatttt ttgatcagtt ctacttttat gactgtaggt
    14701 aaacgatatt ttctaggaca attcgcatca gcttcgatcg aacgaatgaa tcccttttgt
    14761 tcccaatacc ttaattgacg tggagacaca cctacgatat cacttaattc actgatgcca
    14821 acaaccaatt gatcattttc caacaaacgt ttaagggaag ccccaaccat ttttgtcact
    14881 ccttttgtcc tcttgcttcc cttaattata tccgtattaa cttatgtgtc aagtaggttg
    14941 acaaatagcg aatttaggac taaagtattc tagtagtttc agaaaaaatt cagaaagaag
    15001 gagaaatatg agtaacaacc aacccgtaga tatttacgga aaaccatata atcggacact
    15061 tttagttgtt gtcttattaa ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac
    15121 gacagctttt cctacattga tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt
    15181 aacgactggt ttcttgctag tcaacgggat catgatccca ataacagcat ggctgatcaa
    15241 taaattcagt tcaagaaatt tatatctttc tgccatgacg atctttttga tcggaacgat
    15301 tacttgcttt acagcaccta attttagtac attactaatc ggtcgtttga tccaagcttg
    15361 cggtgtggga gtatcgatgc cattattgca aaatatcatg ctatctattt tcccgccaga
    15421 aaaaagagga tcagcaatgg ggatgtcagg gatcgttatt ggacttgctc ctgctttagg
    15481 ccctacatta tctggttata tcattgataa ctaccattgg cgtgacttgt ttggtatggt
    15541 actgccaatc gttgttcttg tcttggtttt agcattcttc ctaatgaaaa gtgttattcc
    15601 attgtcaaat cctagcattg atattttatc cgctatttta tcaacgattg gattcggtag
    15661 tttactttac ggattttcta gtgtaggaga tgacggctgg ggaagcttga aagtaattgg
    15721 tttcttagtt gttggtgtga ttttcatcgc cttgttctca tggcgtcaat tacatttgga
    15781 acatccattc ttggaacttc gtgtattcaa atcatctaca ttcacgattg cagctgtttt
    15841 agctggtgta acaaatatgg cgatggtagg ggcagaaatg gtcgtaccat tgtatattca
    15901 aaatattcgt ggagaatcag cattccattc tggcttgatg ttgttgccgg gtgcattgat
    15961 catgggggct atgatgcctg tgacgggtgc gattttcgat aaatacggcg cgaaacgttt
    16021 agccatcaca gggatgttca ttttgacagc ggcaactgca ccatttgctt tcttgacaaa
    16081 aacgactcca gttttatata tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat
    16141 ggtcatgatg ccggtaacga cttctggtat gaatgcattg ccaaataact tgatcagtca
    16201 tgggacagcg gtaaataata cattccgtca aatcgcaagc tcgattggta cagctatttt
    16261 gatcagtgtg ttgacgaatg tgacaaaagc aaatatccca agcaaatctg tattggataa
    16321 tacgccatta gcttataaag atcaggcaat caatgccaca ctgtcaggtt accatgccgc
    16381 gtttatcgta gcgatcatct ttgggattct tggctttatt attgcctttt tattgaagaa
    16441 aaaagaacct gttctaatga aagcggggga taatgcatga ttattggagt tttgattgtc
    16501 agcttgcttg cttttgcttt aacgaacgtg ttcgctaaaa aaacatggca aaccttttta
    16561 tctttgattt tcggactaat cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc
    16621 aatcactttg gaatggaaaa agtaacggaa acaaaaacag aaaaaattgt ttccagtgct
    16681 gattcccaag gagcagatat gcttttgtat aaagctttag ggaatggaaa agaaaaagtg
    16741 tatctttacc gaacaaatga aaaacaagaa aaaccaaaag caactggtac ggataacgaa
    16801 acaaataaag tagaaaagac agacggcgat gctgaaaaag tgacgaaaac tacttattgg
    16861 gaatacaaaa acgacatgta taaattctgg ttcaatattg ctgataacaa tcacgaatat
    16921 gacagccgag tgaatacatt caaaatccca gagacatggg tagaattaag tacggaccaa
    16981 gcagccaagt tagctgaatt agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa
    17041 gcttatgttc aagacggtat ggtaaaagca atgactgaaa atccaaaaat gagcaaagca
    17101 gagcaagaac aacgtacgaa agccttagct gctgaattcc aacaacaagc attcgcaaaa
    17161 ttagtgaaag aagcaaaagg cgaataatat tttgaacacc gtttaagg
//
LOCUS       DACEKH010000060        16676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEKH010000060 DACEKH010000000
VERSION     DACEKH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.063"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(90..824)
                     /locus_tag="IW662_001817"
     CDS             complement(90..824)
                     /locus_tag="IW662_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0866638.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1150..1560
                     /locus_tag="IW662_001818"
     CDS             1150..1560
                     /locus_tag="IW662_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ0866639.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1553..2254
                     /locus_tag="IW662_001819"
     CDS             1553..2254
                     /locus_tag="IW662_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ0866640.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2254..3867
                     /locus_tag="IW662_001820"
     CDS             2254..3867
                     /locus_tag="IW662_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0866641.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3857..4078
                     /locus_tag="IW662_001821"
     CDS             3857..4078
                     /locus_tag="IW662_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ0866642.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4075..4836
                     /locus_tag="IW662_001822"
     CDS             4075..4836
                     /locus_tag="IW662_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ0866643.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5217..5726
                     /locus_tag="IW662_001823"
     CDS             5217..5726
                     /locus_tag="IW662_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0866644.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5796..6335)
                     /locus_tag="IW662_001824"
     CDS             complement(5796..6335)
                     /locus_tag="IW662_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ0866645.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6475..7086)
                     /gene="rpsD"
                     /locus_tag="IW662_001825"
     CDS             complement(6475..7086)
                     /gene="rpsD"
                     /locus_tag="IW662_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ0866646.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7402..8199
                     /locus_tag="IW662_001826"
     CDS             7402..8199
                     /locus_tag="IW662_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ0866647.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     QGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8227..8862
                     /locus_tag="IW662_001827"
     CDS             8227..8862
                     /locus_tag="IW662_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ0866648.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8882..9655
                     /locus_tag="IW662_001828"
     CDS             8882..9655
                     /locus_tag="IW662_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0866649.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10063..10860
                     /locus_tag="IW662_001829"
     CDS             10063..10860
                     /locus_tag="IW662_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ0866650.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10838..11251
                     /locus_tag="IW662_001830"
     CDS             10838..11251
                     /locus_tag="IW662_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866651.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11235..13880
                     /gene="mutS"
                     /locus_tag="IW662_001831"
     CDS             11235..13880
                     /gene="mutS"
                     /locus_tag="IW662_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0866652.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13898..16006
                     /gene="mutL"
                     /locus_tag="IW662_001832"
     CDS             13898..16006
                     /gene="mutL"
                     /locus_tag="IW662_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ0866653.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16028..16588
                     /locus_tag="IW662_001833"
     CDS             16028..16588
                     /locus_tag="IW662_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ0866654.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5410 a         2978 c         3613 g         4675 t
ORIGIN      
        1 aagctcgagt tacgagtttg gaacagcttt tgtctacagt ctgacttggt acgcaatcgc
       61 tgtaccaagt ttttttattt agcttttgat taagccataa ataataattt atataagaat
      121 actgctacga caccagcaca gataggtgct acaactggta cccatgcata tccccattga
      181 gaagaacctt tatgtttcaa tggaagaagt gcgtgtacaa aacgtggagc aaagtcgcgg
      241 gctgggttta gtccaggacc tgtaggacca ccaagtgaag caactaatgt ccatacaagg
      301 aaaccgagtg ctaagtgagc tgtaccaaga ttatcttgga agaatggtgc ttttgtaata
      361 cctaaggcac caaagaataa aacgaatgaa ccaaagaatt cattgacgaa accattgaat
      421 ttagaaccaa cgctgttgat tgttgagaat gtacctaaca catggttcac gtcttctgtt
      481 tggtcgtagt aaggtttgta acaagctact acgatcaatt gtccaatcgc tgcaccaatc
      541 aattgtgcaa tgatataagg aacaacttca gcccaaggga atagaccatt agatgctaaa
      601 gcaattgtaa atgctgggtt gatgtggtta ccgctgattc caccaaaaat cattgctggc
      661 atcataaccc cgaatccata cccgacagca atcagcatcc agtcactgcc atatcctttt
      721 gtacctttta aatctacgtt tgctacagca ccgttaccta gaacgattag taatgctgta
      781 ccaatcattt ccgccgcaag acgggtcatt agtgagtaat ccataaatac ctccaaaagt
      841 tataaaaatg caaaaaaaaa gaaacatcaa cacgaaaagc atcggtgcag tgtttcgtgt
      901 cggaaggatt tctgcagtct taacaaatgt gaataaaaaa atgaattcca ttacttataa
      961 gaaaccagac caacagtaaa actccaactc tttttaaaag ttctccacaa accaccaagc
     1021 ttaactataa atataccaaa aaaaaaacgt tttttttctc aaaaattgta tcttagaaac
     1081 agcatgtttt gaaactttca gaaaaggttt gttagtaaaa tgaaagctat acgtaaggag
     1141 tgttgtttga tgaaattttt tcaagagttt ttcattatta ttttcttttc tgtattagga
     1201 gaaggcatac ggatttttgc tcatttacct attcctggaa gtatcttagg tattcttttt
     1261 ttatttttag catttgaaat gcatcttgtt gatcctgaaa aaatcggagc aactggtgat
     1321 tttttattga ataatctagc tattttattt gttcctgctg gtgtaggttt attggaatat
     1381 tttgatgata ttgcttcgat atggcctgtt ttactgggag cagtcgtcgt gtgttccatc
     1441 gtcacgatgg ttgcagaggg aaaaacggca gaaggtgtag aagctttgct gggctatgtc
     1501 cgtaagaaaa aagcaggaag ggtatctgta aacgaaatgg aggaaaatca aattgactga
     1561 gttttttgtg aatccactat ttggcttaag tttgacttta ggagtgtatg tattatctga
     1621 taaatttttg cggaaatata aactgcctgt tttgaatcct ctcgtattat cgattgttgt
     1681 tattatctta tttttgcatt tttctgggat cagctataaa gactataata tcgggggcaa
     1741 ttttttgact atgctgatta ctcctgcgac agtggcactt gccatccctc tttataaaaa
     1801 cttccgttta ctgaaagaga atttttatcc tgtaatggct gcgattttag caggaatagt
     1861 cgcaaattgc ttggttagtg tagggttcgg ttacttcttc tctcttcata aagagatggt
     1921 gatctcatta ctacctaaat ctgtaacgac ggctatttct gtcgatttat ctcatacgat
     1981 gggcggggtg aatgccgtga cacttgctat tgttgtctct acaggtattt ttggttcgct
     2041 gatcgccact catattttcc gcctattcaa gatcgaaagt ccagttgctc gcggtgtggc
     2101 gttaggaagt acttctcatg ctatcggtac agcaaaagca atcgagattg gcgaaataga
     2161 aggaatcatt tccggacttg cgatatgcgt aaatgggata ttgaccgttt tgttgctgcc
     2221 gttattcttt cagccttttg ctggcttgtt ttgatgcgaa aaccactatt ttatgctaca
     2281 ataaccaaaa cgttcgagca taaaaaagga gggaaagacg tgtatttgtc ttccgagtat
     2341 cttaaacaat ttgataaaga tctatattat aagattctcc tcctagaaag ttttgaggat
     2401 caagcttggc ataccgctgc acaattagcg caagttgttc agctggatgc ccgcagcgtg
     2461 tcgaaatacc tgaacgagct gtctaaaaac tatcagcagt ttagcggtaa gacacaccct
     2521 ttgtttacaa aaaatcaccg ttctggttat aacttttatg atacgctcga ttcaattgaa
     2581 catgagcgtt ttttgatcta tctcgtgcaa agtacgctga aattccagtt gctgcacgac
     2641 attttttttg aagaattcca tacaatgtac caatttgcgc aaaagcatta cattagtgaa
     2701 tctactgctc atcgaaaaat caatgaatgg aaacagcaac tgcaaactta cggaattcgg
     2761 ctgcaacgag gtacatatat cgctcaaggc gaagaagaaa tcattcgact ttacttgcat
     2821 atgacattct ggcagttgtt tcgagggaaa atctggccat ttgaaacgat ttctcaaatg
     2881 gacgtaaaga atatggcaga acatatcatg gcttttttta atgttcgtct gaacgagatc
     2941 aaaaaaagac gattggaata catgctaggt gccttttttt taagaaaatc acagaagcat
     3001 tatgttgtat tgaatgagaa aaaaagacgg ctgatttctg acaatctgtt gttccagcga
     3061 ttttgccaag tcatggagcc ggtatttcct aattattttc aagtagaaga cgagttaggc
     3121 gcattatttc ttgtactgat gactcgtgaa gaatattata gtgatcctaa gatacggaag
     3181 aaaatttttg attttcatca acaagcaaaa acaccgccct ttacagccct ttcagaagca
     3241 aaagccgctc tttctcttta tcaggaagag caggggctgc cagcagaaaa tctaaccttc
     3301 gaggcagaaa actatttatt cagcagtcat tttttcgcct atctttttcc taatgcgaaa
     3361 gaaacgattg atggcaatag tagtgatttt attaatcatt tagttattga aaacaaggaa
     3421 ttgaaacaat ggctcgtaca tttttttgaa agtagacaca aacatcccaa tcatctggca
     3481 tttaaaaatc atgcgttttt gatgggtcgg tatctgactg tgtttaaaac attaggagcc
     3541 tttactccac agcttccaaa gatcacgatc cttttaatga cagatttccc attatttgaa
     3601 gagcaactgt tagaggaggg attacggaca tttttccgta atgagtatca gctgattttt
     3661 ctgccaacag attatcgggg gagagaagtt gatcttctga tttcgacaag taaagtccac
     3721 cgcaaaccat gggcggatct tgactatttt attgtgaccg aagaactaaa attaattgat
     3781 tacatccaac tatctcaaaa atttgaaatg attcaaaaaa aaactattaa aacaataaca
     3841 aagaaggaga aaaaagatga catttgaaga aattttacca gaattgaaaa aaggtgcaaa
     3901 gattattcgt aaaggatgga gtggatttga actttatgtt gttctcgtat ccgatgatga
     3961 atttgatggc tgtccagtga caccttactt attgatcaaa acaagtgacg aaggtttttc
     4021 cagcttcgct cctacagttt gtgatatttt agcagatgat tgggcgatcg tagaatgaaa
     4081 aaccagtatc cagatctagc taaacaggtt gtatttatca caggagctgc ttctggaatt
     4141 ggtgctgcgc aagcccgagc gtttcttgaa caagatgctt ttgtatttgg attagatcaa
     4201 aagttcggta aaatggaaga actctctttg cgatttcctg aaacttttgc ctacgcacta
     4261 ggtgatgttc gacaaatgaa agaactccaa caagcggtat cgaaatgcca ggatcgtttt
     4321 ggtgaagtta cgattttact gaatacatcg gggatattgg atgcctacaa accattgatg
     4381 gaaactgacg aatcgctgtg ggatcttatt tatgagacga atgtgaaaag catgtatcag
     4441 ctgacaaaaa tcatccttcc tgatatgatc cgccgaaaaa gcggaacaat catcaacatg
     4501 gcttcgattg cgggtttggt tgctggcgga ggtgggatcg cttatacaag tgcgaaacat
     4561 gcaatcgtcg gatttaccaa acaactcgct ctggattacg caggacaggg tatccgagta
     4621 aaaggaattg ctccaggagc catccaaaca ccaatgaatg cggctgattt tgaagggaaa
     4681 ggtgagatgg ctgagtgggt cgcaaatgaa acaccagtca agcgatgggc acagccggaa
     4741 gaagtagctg agctgacgtt atttcttgcc tctcctcaag caagctacat acaaggaacg
     4801 atcatcccaa tcgatggcgg atggttgtta aaataacctt gtgttttttt caagcttctg
     4861 tatgataaaa ctaaagagag aaacaacggt tgatgataaa agaaagtcga tgactgttcc
     4921 cgacattcga tgccgaacag ccagttcaaa aaattctttt taataatcgt taatattcga
     4981 aactaagcgg cttttcacga ccttttgata taaacggtgt tcaaaagctt gcaactgcgg
     5041 taacaagtcc agccaaatga aaaatataag gagctatttt tatttgacta ttcttattac
     5101 ttgttgcagg gcagcttttt cactaccttc tgcggcactt cagttggaat tttcttccca
     5161 tcctttggtc gcagtcatta ccaagataaa attatcttgc aaaggagaaa tagaacatgg
     5221 aaaaaaataa tacccgttca gccaaacgct ggacaacgac agatgtgact cggatggcgc
     5281 tagtcactgg tttatatgtg gcagtgacgc tggttttgtc tgtctttagc tttggagtga
     5341 ttcagattcg tctctcggag atgttcaact atctgtcctt gtataacaaa agatacatcg
     5401 tcgcggtaac agcgggagta gctttggcta atatggcttc tcctcttgga ctgatagatg
     5461 ttgtagtagg aagcgtcagc acgctgcttg tattgcttat caactataaa atcactagtc
     5521 gcatcaaaaa catgaaaatc aaaatggctg tgacagcatg cgtatttgct ttttccatgt
     5581 ttacagtagc tggtcagcta acgattttat atcaactacc ttttttcttg aattggtggg
     5641 tgattgcttt aggagaattg gcatcaatga ttgtcggtgg gataattatt tattggatcg
     5701 gtaaaaaagt ggatttgtca aaataaagat ctgtttaatt taaaatatgg cagcgtggaa
     5761 gagactttga gtcttcttcc atgctgcttt tttgattaat ttgtatgaag aaatcgattt
     5821 agccgttttt tcatcaagat accgatataa gctgctccag ttgccttgat tgcttccggc
     5881 agtaaaaacg gcagtagtcc actggcaaat gcagttgtaa aagtaagatc accacttatt
     5941 ttcagccaga tcgttccgca gatcaatgca gcaagagagc ccattagatt ggcaaagatt
     6001 gcatataagt aatgagcatc cgttctttcg atcatccaac cagttatcca cgtttgtaag
     6061 agaaatccaa tcaaatatcc gccagttggc ccaaataaaa cagctatacc tccagtcatc
     6121 cctgaaaata caggaagacc aatcaagcca agtaaaagat aaactacaac agatatcaat
     6181 cctagttttc ttccgagaaa agttgctgtt aatccaatag caaatgtttg ccccgtcaaa
     6241 ggaaccaagc caaaaggaat ggtcatctga gaaaggacag aaataatcgc ggcaaattcc
     6301 gcagcaagta ttaattcttt caatgttcgg ttcataatgc cctcttttcg ttaacttaat
     6361 atttttattt ggttaacgaa attgtaaaac aaagtgatta ctttgtaaat agaaaatata
     6421 aaaaaagacc agctatccaa tagatagcgg tctttttcaa gaaagctttt gaaattataa
     6481 gctttggttg tagtattcaa cgacaagagc ttcgtcgatt tctggatata gttcgtcacg
     6541 ttctggtaaa cgagtcaatg aaccttccaa tttttcagcg tcaaagctta cgaatgctgg
     6601 gcgtccaaca gttgcttcta cagcttcttt tactgttacg atgttttgtg atttttcacg
     6661 aactgagatc acttgaccca cttctacgtg gtatgaaggg atatcaacgc gtttgccatc
     6721 aactaaaatg tgaccatggt taactaattg acgtgcttga cgacgagtcg ttgcaagtcc
     6781 taaacggtaa acaacattgt ctaaacgttg ttctagtaag atcatgaagt taacaccatg
     6841 tttaccttct ttgattttgc tagcttttac gaacaagtta cggaattgac gttcgttcat
     6901 tccgtacatg tgacgaagtt tttgtttttc agttaattgc atgccgtatt ctgattgttt
     6961 gccacggctg ttaggtccat gttgacctgg tgcgtaaggg cgacgtgcta attcttttcc
     7021 tgtgcctgac aatgagatgc ctaagcgacg agaaattttc catgatggtc ctgtataacg
     7081 agacattaaa atttcctcca atgaatataa gtttttggag taaaataatc cgttgaaaaa
     7141 ttcataatca tatagtttgt tcttcaatct tcacctttgc agccgcggtt acacaatcga
     7201 acccgagacg gggcaacaaa ctagggatga gttattattt ttctgctgca ttattttaca
     7261 caaactacag tatatgatag atcatccacg aatgtcaaga taattagtac ataaaaacgc
     7321 tttacaaacc taggctgtct ttataaaatg agagggcatg tgcaaatgga aaaaagcgaa
     7381 ggaagtttaa ggagaaaatc aatgaatccg gtatcaccat tatttgagaa aatcgatgct
     7441 tcaattgaaa agaaggcgaa tctttttgaa aacagctttt cacgttacgc tgttcgagcg
     7501 gttctcgcct gtttgttttt gactttagga acggctgtgg catttgcaat cgcgatgaag
     7561 ggggaagata tcgcgcatgg tctaggaaag attttatacg catttatgtt cagctggggt
     7621 ctggtcatga ttctatacat gaacgctgaa ttaggaacat cgaatatgct ttatatgacc
     7681 gtgggtgttt ttcggcagag acttgcttta agtaaggcag ccaagattct atttgcctgt
     7741 atttttttca atatgattgg cggtatatta tgtggatttc ttatttctat gactacgcca
     7801 tttcaagatt taacgttgga caactttatg ttagaatcaa tagcagtaaa actagcaaaa
     7861 cctgcagcaa cgattttggt ggaagggatg tttgcgaata tcgttgtcaa taccgctgta
     7921 ctgatcagca tgcggatgaa agaggatgcc gggaaagtga tcacagtcgt attcatcatt
     7981 ttcatttttg cttttttagg atatgaacac gtgatagcga attttcctgc ctttacgctt
     8041 gcttactttg cttctcaagg acagacggat ggaatgacag tcagcaatgt gttacataat
     8101 ttgttttttg ctttggcagg aaattatatt ggtggcggtt tagtaatggg actgggatat
     8161 gcttggctga atcagtcaaa atcatcctat gtggactaaa aagtcgttca ttaaagggga
     8221 agtgccatgg aagttttagt ttttccaaaa aatgaggaaa aagcgttaca aatggaaaag
     8281 tacatgaaaa atcttttttc ctttgctgga gtacctaagc cggaacgcgc tcttttacaa
     8341 caaccgttac tggatgagag caaaaattta tctatttctg aagttttagc ttttgtttat
     8401 tattattatg aacaaatcga aagagagtat cagtatgtag cgatcgattt agtccagaaa
     8461 aacatcaaac ggcttacatt tgaagactta gtcgtattgt tgccactagt tcagaaaaaa
     8521 gaatggtggg acagcatcga ctcttggaga aaagtataca gcacatggtg taaagcctac
     8581 ccagagcagc gtgaagaagt ctttcaattg tttgaaaaaa aggaagattt ttggctacgt
     8641 cgaatagcaa tcacgttgca attaggttat aaacagcaaa cagacacagg actattgatc
     8701 cgagcaatag acgaagatcg tcaaacaaag gaattcttta tccaaaaagc aattggttgg
     8761 gcattacgcg attacagtaa gaccgatgcc gattgggtaa aaagacagct gacaacagat
     8821 ttgagtaagt tagctgttcg agaggcaagt aagtatttat aagaaagcga gacgacaaga
     8881 gatgagacgc aaattatttg cttttgatat tgatggaacg ttgttagatt caaacaaaca
     8941 agctttagat agcacgagag aagcattgga aaaattacgc aaagccggtc atctggtcac
     9001 gattgctaca gggcggagcc gatttcatgc gcaagagatc attcgcgatc ttgattttac
     9061 gaactacatt ttatgtaatg gagcggccgg ttttttagac catgaacaag tatataagaa
     9121 tttactagat cgcgaccagt tggatgcgtt cgttcaagaa gcgcatcatg agcagatcga
     9181 tacagctttt gtcagcttag acaatatcaa gcgttcttct tccaatgatg taacggtgat
     9241 ggaagaagcg atgcattctt tcggtgctgt cttgcctgaa ttagatcagt tttttgcaga
     9301 ggaacaagaa atataccaag cgttagcatt tttcgatcaa acatatgatg ggaagttctc
     9361 ttcatatgat aaaattcgat ttgtccgctg gcatgagaac agtgtagatg tcgtacctaa
     9421 aaatggttct aaagcagcga caattctgca cttagcagaa caagtaggca tccctcatga
     9481 agacatcatc agctttggcg atggacaaaa cgaccgtgaa atgttgcgca tgtcaggaat
     9541 tgggatagct atggggaatg ctgtgccaga agtacaagca gaagctaaga tggtcactga
     9601 tacaaacgat caggatggaa tttggaaggc attgaaagag ttaagcgtga tttaaaagag
     9661 gttgtgaaaa agctgttctg catcaagtaa taagcccagc caaacaaaaa tagctcctcc
     9721 tatttttcgt ttttctgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga
     9781 agaggtcgtg acagaagtgg tcagtttcaa gaaataagaa gggatttacg aaaattgctt
     9841 ctcaaatttt tgtaaaatta cggcttattt ctgaagaagt agatgaaaca acgtttattc
     9901 gggaaatggg actgtgacaa gatgtttgtc atagtcccat ttttttattt tatttttcaa
     9961 aactaagaaa taccaggaaa gagttccttt tgtttgacct tttctttctt attgaaaaca
    10021 actataatat agagtgatgt taaaagagaa gaggaattta gtgtgcgggt attgtttatt
    10081 ggtgatgtag taggttcaat gggacgagag atgattactg aatatcttcc tagattgaag
    10141 aagaagtacc gtccgcaagt aacgattgta aatggtgaga atgccgcttc tggacgtgga
    10201 atcacagaaa aaatctataa aaaatttttg caagacggcg tagatgtcgt gacgatgggg
    10261 aatcatacgt gggacaaccg cggaatcttt gagtttgtaa atgaagctaa aaagatggtt
    10321 cgtccggcaa attttccgga agggacgcct ggacaaggga tggtctttgt caaagtgaac
    10381 caagtcgaac tggcagtgat caacatgcag gctcgttctt ttatggtgga cttggatgat
    10441 ccttttcgta aaatgaatga gctggtagaa gaagccagaa aacgtacgcc gatcattttt
    10501 gtagattttc atggagaaac aacaagtgaa aaacaagcaa tgggctggtt tttagatggg
    10561 aaagtttcag cagtggtcgg tacacataca cacgttcaga caaatgatgc gcgaattttg
    10621 ccaagaggaa cagcgtatct gacagacgtt gggatgacag gtccctatga tggtattttg
    10681 ggtatgcgca gagaacctgt aatcgaaaag tttctgaccg cattaccaaa acgttttgaa
    10741 gttgtggaac aaggccgatc gattttaagc ggctgtatct tggaattgga tgatacgaca
    10801 ggtcatgcaa aaaaaatcca gctgattcag atcaacgatg atcgaccgtt catggagtag
    10861 aaagagaggc agttttttgc agcaggagcc ggaaattcaa aaagaagtaa ggaaactgac
    10921 aaaactttta agagagaatg aaacgatcat tcgctacaaa gaacttgaag aaaagatcca
    10981 gcaaaaccag tatttagctg agttgagaga aaaaatcaaa caagcgcaaa aagatgctgt
    11041 tcattttgcc cactatgaca aaccagcagc agaacaagaa gcaatcaaac aagcggatca
    11101 attcatgcaa gaatttgatc agcatccgct tgttgtagct tatagaaaac agctgttgga
    11161 agcagatgat ctgcttcatc atttaacaac tatgatacaa gaagaaatca atggacaaat
    11221 agaggaggaa aaacatgcct caaaaaacta aaaatactcc tatgatggaa caatatctaa
    11281 gcatcaaggc ccaataccaa gatgcttttt tgttctatcg tttaggagac ttttatgaac
    11341 tattttacga agatgcgatc aaagcgtcac agcttttgga attaacgttg acgagcagaa
    11401 atcgaaatgc agaagaacct attcctatgt gcggtgtacc tcaccatgca gcacaaggat
    11461 atatcgatgc cttgattgaa aaaggctata aagtagcgat ttgcgagcag gtagaagatc
    11521 caaagacgac taaagggatg gtcaagcgcg aagtgatcca gctagtcact cctggtacag
    11581 tcatggacag caaaggattg tcggcaaaag acaacaattt tttaacagct gtagttagtc
    11641 aaggaaatga atttggcttt gcttatgccg atttaagtac gggagaattg aagacggctc
    11701 ggttgcatga tgaagaagct gttttaaatg aagcctctgc attgcagaca aaagaactcg
    11761 tattaggcag tgagatcact gatacccttc gtgaacagct aagcgggcgt ttagggctag
    11821 tcttttctca acaaaatgag atggaagaaa acgcggaatt tagctttttg acaagcgaac
    11881 tagtcgatcc gctgaaaaaa gaagcaaccg gcaaactatt gacttattta tcggtgacac
    11941 aaaagcggag tttagctcat atccaaaaag cagtagaata ccaaccagat cattttttga
    12001 aaatggatta ttattcaaaa ttcaacctag aattaagcca atctattcgg actgggaaaa
    12061 agcacggcac tcttctatgg ttgttagatg aaacaaaaac agcaatgggt ggccgtttac
    12121 taaaacaatg gctagaccgt ccgcttatcc aggcaaaaca aattaaagca agacaagaaa
    12181 tggtcgcttc tttgttagac agctattttg aacgtatcga tttgcagtca gcattaacga
    12241 aagtctatga tctggaacgc ctagcaggtc gagtagcttt cggtaatgtc aatgggcgag
    12301 acttgatcca gctgaaaaat tctttagaac aagtgccaat gatcagaggg ttgattgaag
    12361 gaatcgatca aggtcaatgg caaagtctgc tttcagaaat gcagccgatg gaccatctcg
    12421 tccaattgat cgaccaagct atccaagatg atccgccgtt acaaataacg gaaggaaaca
    12481 tcatcaaaga tggattcaat gagcagctcg atatgtaccg ttctgctatg agaaacggaa
    12541 agaaatggtt ggcagaactt gaagcaaaag agcgtcaaga aacaggaatc aaaaacttaa
    12601 aaatcggatt taaccgcgtt ttcggttatt atatagaaat cacaaaagca aatcttggaa
    12661 atgcagaact ggagaagtat gaacgaaaac aaactttagc aaatgcagag cgtttcatca
    12721 caccagaact aaaagaattg gagacacaaa tcctcgaagc agaagaaaaa tctgttgatt
    12781 tagaatatca gttgttttta gctgtgagag aagaagtcaa aaaggctatc cagccgctac
    12841 aagtacttgc aaaagcaatc agtgcagtag atgtgctaca aagttttgca acgatcagcg
    12901 aacgctatca gtatgttcgg ccagaacttg tcagtgacaa gcatcagctt ctgatcaaag
    12961 acggacgaca tcctgtagta gaaaaagtat taggacatca ggaatatatc cctaacagcg
    13021 tagaaatggc agaagacgaa atgatcttgc tgattacagg accgaatatg tctggtaaaa
    13081 gtacgtatat gcgacagttg gcattaactg ttctgatggc gcaaatgggc tgtttcgttc
    13141 cggcaaaaca agcagtttta cctatttttg atcggatttt tacccgaatt ggtgcaagtg
    13201 acgacttaat tgctggacaa agtactttta tggtagagat gatggaagcc aatcaagccc
    13261 tgcgttatgc tacgccaaac agtttgattt tgtttgacga actagggcga ggaacagcaa
    13321 cctatgacgg aatggcgttg gcccaagcaa tcatcgaata tatccatcgt catgtacaag
    13381 caaaaacact cttttctact cattatcatg aactgacggt acttgagaaa gaacttccgc
    13441 agcttaaaaa tgtccatgtt ggagccgttg aaaaagacgg agaagttgtc ttcttgcata
    13501 aaatgatgga tggacctgcg gataaaagct acggcataca tgtggcaaag atcgcaggat
    13561 tgccttccaa tctactagaa cgagcagcag atatcctgca tacgctagaa aatggcgata
    13621 acggacacca tactgttcca gctgaagtag aagagaaaac taaaacagcc aaacaggagc
    13681 aaataaaacc acctgcagaa aaagtaaatc aagaccatat acaagaccgt gtaaaagaag
    13741 aaaaccaaca gctttcttta ttcagtgaat tatccgaaaa tgaaacagaa gtcattgaat
    13801 cactgaagca aataaattta ctagaaatga cgccaatgga tgcactaaac acattatatg
    13861 aattacaaaa acaactgtag gaagaaggcg gatatcgatg gccaaaatcc aagaattatc
    13921 tgaacgttta gctaatcaga tcgctgcggg agaagtagtg gagcgaccag cttccgttgt
    13981 gaaagaatta gtggaaaatg caattgacgc cggcagtact cagattgata tcttagttga
    14041 agaagcggga ctgcggacga tccaagtgat tgataacgga gaaggcattt tggcagacga
    14101 cgtagagaat gccttcaagc ggcatgcgac aagtaagatc cataatcgcg acgatctttt
    14161 tcgcatacga acgctgggct ttcgagggga agctttgcca agtattgcct ctgtttctga
    14221 aatgactctg gaaacggcga atcaggaaga aaaacaaggc acctatctgt cgctgaaagg
    14281 cggagaagtt attgagcatc gaccagcacc actgcgtcaa ggaacgaaga ttaccgtcag
    14341 caatctgttt ttcaatacgc cagcccgctt gaaatacgtg aaaacactgc acacagaatt
    14401 agcgaacatc ggggacatcg tcaatcgact ggcattgagt catccatcga ttgcttttcg
    14461 tttagtacat gatggaaaca aaatgctggc aactgctgga aatggcgatc tgaaacaaac
    14521 aattgcggga atctacggat tatctaccgc aaaaaaaatg ctgaaaatcg aaggaaaaga
    14581 tctagatttt gagttatttg gctatgtttc attgcctgag gtgactcgtg ccagtaggaa
    14641 ttacttatcg acgatcatca atggacgttt tatcaagaac ttttccttga ataaagcaat
    14701 cgttgcaggt tatgggtcaa aattaatggt cggtcgtttc cctattgctg tattggaaat
    14761 caaaatggac ccgcttttag tagatgtaaa tgttcatccg acgaagcagg aagtccggct
    14821 gtcgaaggaa aaagaactaa cagagctaat tagtaacgcc attcaaaaag cattgtctca
    14881 agaaaatctg attccaagtg cagcggataa tctacggttt aagaaaaaag tacctgatca
    14941 gcctaaaaca gaacaaatcg aaatcgattt gtcctacgct tttgaagaag aaaaacctaa
    15001 aaagccaagt agtttgaatt ttgatcgaga gacaggtacc tttttcgtag aagaaccaaa
    15061 gaacacattt gttgacagaa ttgttgataa gtctgtggaa aatgttgaaa actattctgg
    15121 tttttctgaa gaaaacacca cttatccaga aaaaacagcg aaaacggtgg ataagtttgg
    15181 tgaaaatgtt gataactttg tagaaaaaac agtggaaaaa tctgaaaaga aagaatttcc
    15241 cgttgtggat aacatggaga aaacaaatga agaacaagca gataaatccg tagaagagga
    15301 tgaaaagatt tacgaggaag aattgacact ccatcctgaa tttgatttca ctcaaggaaa
    15361 cgacaaaaca ttagaacaag ccatcaagaa attagaaacg gaaaaaccag tacaacgttt
    15421 tcctcagttg gaatattttg gacagatgca cggaacgtat ctttttgccc aaagtagcga
    15481 tgggttgtac atcattgatc agcatgctgc ccaagagcgc attaagtatg aatatttccg
    15541 ggaaaaaatt ggggaagtat ctgatgatct gcaagaattg cttgttccat ttgtgttaga
    15601 ctatccaaac agtgatgctt tgaaattaaa agagcaaaaa cacttgctag aagatgcagg
    15661 gatttattta gaagaatttg gtcaaaacag ctttattgta cgggcccatc ctacttggta
    15721 tcccaatgga gaagaagaat cgattattcg ggagatgatc gatatgctgc tgacaactgg
    15781 aagtgtgagt gtaaaaaaat tccgtgaagc aacagcaatc atgatgagtt gcaaacgatc
    15841 gattaaagcc aaccattatt tgaatgaagc acaagcacgt gtgctgctga aggatctggc
    15901 acgatgcgaa aatcctttca attgtcccca tggccgtcca gtattgattc actttaccaa
    15961 ttcggatatg gaaaggatgt tcaaacgaat ccaagacccg cattaagtca aagagaagag
    16021 ggaaaacatg aagattgtat tagcatccca atcaccaaga agaaaagagt tattggctca
    16081 tatcgtttca gaattcgaga ttgaacctgc tgatatcgat gaaacgccat ttccagacga
    16141 acagcccgtc gattatgttc gtagaatggc tgaagaaaaa gcacgaattg tctgggaaca
    16201 agatgaaaaa gaagaccatt tagtgattgc cagtgacacg actgttgttc tcaatcaaga
    16261 gatcatgggt aaaccagaag atctggctga ggctgaattc atgctgaaaa aactaagcgg
    16321 ggaaacacat actgtatata cagctgttgt tttaaaaaca aaggaacgag aagaacggat
    16381 actagcagaa gcacatgtca ctttttatcc tttgacagac gaagagatca aacgctactt
    16441 ggagacgggg gattatgcgg ataaggcagg ggcatacggc atccaaaatg ctgcgagtgt
    16501 attcgtcaaa gaaatttctg gagattatta cagtatcgta ggatttccga ttggtgctgt
    16561 caatcaggca ctgaaaacat tcatttaaac aaaaaagcgg actatctgat tttcgattga
    16621 aaatccatga tagcccgttt tttttattga aaagattact tatctactct gactaa
//
LOCUS       DACEKH010000061        16526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEKH010000061 DACEKH010000000
VERSION     DACEKH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.064"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(138..413)
                     /locus_tag="IW662_001834"
     CDS             complement(138..413)
                     /locus_tag="IW662_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ0866655.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(656..1966)
                     /locus_tag="IW662_001835"
     CDS             complement(656..1966)
                     /locus_tag="IW662_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0866656.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2148..3377)
                     /gene="rpsA"
                     /locus_tag="IW662_001836"
     CDS             complement(2148..3377)
                     /gene="rpsA"
                     /locus_tag="IW662_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0866657.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3512..4192)
                     /locus_tag="IW662_001837"
     CDS             complement(3512..4192)
                     /locus_tag="IW662_001837"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0866658.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4241..4876)
                     /locus_tag="IW662_001838"
     CDS             complement(4241..4876)
                     /locus_tag="IW662_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0866659.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4942..6345)
                     /locus_tag="IW662_001839"
     CDS             complement(4942..6345)
                     /locus_tag="IW662_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0866660.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6332..7363)
                     /locus_tag="IW662_001840"
     CDS             complement(6332..7363)
                     /locus_tag="IW662_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866661.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7394..7615
                     /locus_tag="IW662_001841"
     CDS             7394..7615
                     /locus_tag="IW662_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0866662.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7780..8517)
                     /locus_tag="IW662_001842"
     CDS             complement(7780..8517)
                     /locus_tag="IW662_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0866663.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8670..10010)
                     /locus_tag="IW662_001843"
     CDS             complement(8670..10010)
                     /locus_tag="IW662_001843"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0866664.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10247..11770)
                     /locus_tag="IW662_001844"
     CDS             complement(10247..11770)
                     /locus_tag="IW662_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0866665.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12148..12759)
                     /locus_tag="IW662_001845"
     CDS             complement(12148..12759)
                     /locus_tag="IW662_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0866666.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12882..13007)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13089..13805)
                     /locus_tag="IW662_001846"
     CDS             complement(13089..13805)
                     /locus_tag="IW662_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0866667.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13811..14380)
                     /gene="scpB"
                     /locus_tag="IW662_001847"
     CDS             complement(13811..14380)
                     /gene="scpB"
                     /locus_tag="IW662_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0866668.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14364..15161)
                     /locus_tag="IW662_001848"
     CDS             complement(14364..15161)
                     /locus_tag="IW662_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0866669.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15131..15502)
                     /locus_tag="IW662_001849"
     CDS             complement(15131..15502)
                     /locus_tag="IW662_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0866670.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15521..16408)
                     /gene="xerD"
                     /locus_tag="IW662_001850"
     CDS             complement(15521..16408)
                     /gene="xerD"
                     /locus_tag="IW662_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0866671.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4642 a         3577 c         2798 g         5509 t
ORIGIN      
        1 aattaaataa aatacatgtg ttttctatgt atgacatacg gattcggaat ctctgtctct
       61 tatttattcc ttttctgcta ggaaataaaa agaaaagcac ttgtagccct agagccacaa
      121 gcgctttttc aactgtctta tttaacagcg tcttttaatg ctttacctgg tttgaatgca
      181 ggtactttgc tagctggaat ttcgatttct tcaccagttt gtgggttacg acctttgcga
      241 gctgcgcgtt cgcgaacttc aaagttacca aaaccgatta attgaacttt ttcgccgtta
      301 gctaaagcat cttgaatagt tgaaaataca gcatctactg ctgcagttgc atctttttta
      361 gttaaatcag tagctgaagc aactttttcg attaattctg ctttgtttgc catggataga
      421 ttcacctcct ccacaagaga tatgatagaa atatcattta gttaaaactt tctgagtggt
      481 ccagaagttt aaaaagaaat attgattaac ttcaatattc tgttatcacg atatcatatg
      541 aaccgcttaa tagcaaggtt tttaccaatt ttcacatgct ttttttagtc atagaaacgt
      601 aacaaacgaa actgtctttt tcttttgaaa aaaaaacgtc gatatggtaa aatgcctatt
      661 ttctcctacg agggataatt cggatggacg tcccttcaaa agtaaatgct tttctgattt
      721 gattttctaa gaaacgttca taagaaaaat gcatcaattc ctcttcattg acaaaaacga
      781 caaatgtagg tggtttgaca gcgacttgtg tagcataaaa aatcttcagg cgttttcctt
      841 tatctgttgg tgttggattg attgctacag catccataat gatatcattc aacacagccg
      901 aagagatacg aagattctgg ttcatactta cttgttcgat catttcaggc agacgttcca
      961 accgttgttt agtcactgca gaaacaaaaa cgattggagc gtaatcaaga taccggaatt
     1021 catcacgaat ctcttgttcg aaatcacgca ttgtattggt ttctttcttt acaagatccc
     1081 atttattcac gacgataata attccacgac cagcttcatg ggcgtaaccc gctactcgct
     1141 tgtcttgttc ccgaatccct tcttcagcat tcagtaccat caaaacgata tcagaacggt
     1201 caatggcacg cattgcacgc atcacgctgt atttttcagt attttcgtat acttttccgc
     1261 gtttacgcat accagctgta tctatcatta agaatttctg accactttca gaaacgaagt
     1321 gggtgtcgat tgcgtctcgg gtcgttcctt caatatccga aacaattaca cgttcttctc
     1381 ctaaaatcgc attgatcaaa gaggatttac caacatttgg gcgaccaatc aaactaaact
     1441 taatcgtgtc gtcgtcctct tcttcagtct cttcagagaa atacttcact gcttcatcta
     1501 aaatatcacc aatccctagt ccatggcttc ctgaaacagg ataaggatcg cctagcccta
     1561 aggaataaaa ttcatagata tcgtttctca tttctggatt atctacttta ttgactgcta
     1621 aaatcactgg tttattactt ctgtataaaa ttttcgcaac catctcatct gcgtctgtca
     1681 cgccttcgcg tccgctgact acacaaatga ttacatctgc ttcttcaatc gcgatttctg
     1741 cttgatgctt gatctgatcc ataaaaggtt catcgccaag atcaattccg cctgtgtcga
     1801 tcacgctgaa ttctcgacct aaccattcac cttttgcata aatacgatca cgagttactc
     1861 caggagtatc ttcgacaatc gagatacgct caccagcgat gcggttaaaa atcgtagact
     1921 taccgacgtt cgggcggcca acgattgcaa ttgttggatt tgccatatta tttcctcctc
     1981 acaagtttcc aatctcactt agtttaacga atgcattgcc aactttcaag catagattgt
     2041 caattaaacg aagtattttt attccttatt gaaatactaa tcgcttttca aaagaaaaac
     2101 tgagacaaaa gcagctgctt tcatctcagt cacagataca aaatcaatta tttttcagaa
     2161 tcatcggttg ctttttcttc tacttcttct ttcaacgcat cacctaaaat gtctcccata
     2221 gtaaatccag tattttcttc tggtaattca taagactcaa cattttttgg ttcttcttct
     2281 gaagctggtt tttcttctaa agctttgata ctcaatgcaa tacgatgttc ttctggatgg
     2341 atctctaata ctttcacttt cacttcgtca ccttcatgaa gcacttcatg tggtgtcgca
     2401 atatgtttat gtgaaatttg agagatatgg actagccctt ctactccagg gaacacttca
     2461 acaaaggcac cgaagcttgt caaacgttta actgttccgt ctaatactgt gccaactgga
     2521 gcttttgtat cgatttctga ccaaggtcca ggctgtgtgt ctttgattga caaggaaaca
     2581 cgttcttgtt caggattgat agacaacact ttgacattta catcatcatt gacagccaat
     2641 acatcacttg gttttgctac atgactatgt gaaatctctg agacatggac tagaccgtca
     2701 attccgccta ggtcgacaaa tgcgccaaaa tcagtaagac gtgcaacacg tccactgaga
     2761 acatctccct catggatagt tgaaagaatt tcttttttct tttcttctct ttcgttttcc
     2821 actaccgctt tatgcgataa gatcaaacga ttttctgatg gttcgatttc gatgatttta
     2881 aagctcaatg ttttgccttt atagtctgaa aagtctgcaa caaaatgatc ttccaccatt
     2941 gatgcaggga caaaaccacg tacaccgacg tcaacgacta aaccgccttt gacaacattt
     3001 gtaacaggtg cttcgatgac cttgccctct tggaaatctc tttcgatatc ttcccaaact
     3061 tttttagcat ctaaacggcg ttttgaaagt aggtagctgc cgttttcttt atctttgccg
     3121 atcgtactga taacgactaa atctaataca tcaccgactt ttactacttc gctgatatct
     3181 tctaccggca aagttgataa ttcttttgcc ggaacgactc cttcaactcc agccccttcg
     3241 attccgacaa tcacttgttt gtcttcaatg actaaaactt cgcccttaac aacatcgcca
     3301 acttttactt cttgtacgct tttcattgcg tcttccattg actggttgtt ctcttgattt
     3361 tgatcaaatt ctgtcataac acttgtcctc ctaaccaaca ttctgccgta agtacggtac
     3421 tgtttttagt ttaattgatt attcaaaaaa aataaagcga tttcgttcgt tttccttgaa
     3481 aaaacaattg tttttcattt tttttcttaa tttaaaaaag aagataacct tttttcaaaa
     3541 caacagcttt tattgctgca acgacttctg gaatcgattt gccagtcgta tcgatacgaa
     3601 cagcatcctc tgcttgcttc agcggagaga cgtcccttgt tgaatcataa tggtctcttt
     3661 cctgtatctc tttcgtcagt gtttcaagat cagtcggaat tcctttttct tggttctctt
     3721 tgaaacgtct ttctgcacgc tcacttacgc ttgctactaa aaagattttc acttctgcat
     3781 tcggtaatac agctgtcccg atgtctcttc cgtccatgac aacgccaccg cgttccccta
     3841 tttttcgttg gacagcaact aattcttctc tgacttttcc atgtgcagag acttcagaaa
     3901 cagcttttgt gacatctggt tgacggattt ctaaagtcac atcttttgat gctatgaata
     3961 catgctgtcc atcttcttgt tgcgaaaaag agataggata ctttttgatc agcgaaacta
     4021 gcccttcttc atctgaaaaa gatacattgt tcgtaatagc taaataagtt actgcgcgat
     4081 acatggcacc ggtatctgta taaatataat tcaattcttt tgctaatatc ttggcaacgg
     4141 tacttttccc agatgaagca ggcccgtcga ttgcaatact gatttgtgtc atgatataac
     4201 tcctttttct cagaaaaaaa gccgcaaagc gcggctatta ttatttaatt cttaattctt
     4261 gaccagggta aagcataaag ttgtttggat ctaagccatt caattcaaac aattgatcag
     4321 tggtgatccc tgcacgctca gccacttgtt ttggaccttc gcctgcttga acagtcgttg
     4381 ttgaaccggc tgcagctgat gaagaaggtg ttgtttctgc tgcattgctg cttgaatccc
     4441 ctgcttgata agttgcctca cttgaggaag tcggtgttgt ctctgctgca atactgcttt
     4501 ctgacgttac acctgtatct tgagacacag tagattcttc cgcgattgta ctagattcta
     4561 ctgttgaagt ctcagttgac tcctcttttg ttgtacttgt agaactttct actactgatg
     4621 tcgaagtttc agaagaagtt gctgctgttg cattgttgct tgatccattt ctgatccaga
     4681 aataagcacc tgctggaata gcgatacata agaccagtaa aatcaaaaat agcgaaagaa
     4741 ataatgtatt tcctttcttt tgttgtctct gagaacttct tgatgaaact tcatcatctg
     4801 tatcgtagat tggttgttcc catggttctt gtgtttcgtt attttctgta gattgatgtc
     4861 tatcttttct gctcactgtt ttacctccta aactctatcg tgtgtattat accataggaa
     4921 aaagtgtctt gtcctaaaaa atcaatgttc catgtttttt ttaaataaat ttagtaaaat
     4981 cgtttcccaa tgaagttgtt cagtcttaga aaaaagcttc ctctttggca gaagttctgc
     5041 ttttgctcca tcaatatcac aacaattctg cggcttttcg gagagttttt cgccaaaata
     5101 agctaagatg aattttctgc ggcagttttc ttcattaata tagcgaagca tctgctgcag
     5161 gcggaattct ttttcttttt cctgacgttt gagccgttcc acccaatttt ctggtttttc
     5221 agactgctgg atcagctcca tccatttttt ttgcagctca tcaaagggag cttgctttgt
     5281 ggcatattcc aaataaagct caaaactttg tcgctgttct ctagaaagtt gattaaagaa
     5341 ataatggata cgttcatccc cactttgata aaaaagaatt gctgcacttt cttcctgatc
     5401 tctgcctgct cgaccgattt cttgtacata gttttctaga ctgtctggca aatcataatg
     5461 gaccacataa cggatatcag atttatcgat ccccatacca aatgcattcg tagcaatgag
     5521 aaactgcagc tgatttttta caaactgctg ttgaagttgt cttctttgag ctgcatcaag
     5581 accaccgtga tagtatccaa ctgtaaaacg ttccctgaat aaatgataca atcgttcgac
     5641 ttcttttttt gttgcacaat aaatgattgc cgctccttgt gctctttcca tgaagtgttc
     5701 aagttcttgt tctttttgct gcgttttccg aacaaaaagc gagatatttt gccgattgac
     5761 tgattgtctg atctcttttg gtggtcgatc aaatagtaca tgacgtatat ctcttgctac
     5821 taaatctgtt gctgtcgccg ttagtgccag tgtgaccggg tttcctaaaa gctttcggat
     5881 ttttcctaac tgttgatatt ctggacggaa atcaactccc cattgagaca cgcaatgagc
     5941 ctcatctact acatataacg caatattttg ttttttcaga tgctctaaaa gagaaggatt
     6001 agtcaacatt tcgggactca aaaacaaaaa tttatattgc gatagatgac ggaggacgta
     6061 tcttctttca gattgcgaaa gtaaactatt gaaagcagct actcgtttct cccctcgttt
     6121 ttgtaattgc gtcacttgat cttccattag agaaagcagc ggtgacacga tgatgaccag
     6181 accttccatt aaataccccg tcaattgata gcaaaggctt tttcccgttc ctgtaggcaa
     6241 aatagcgaga gtatcttctc catcaagcaa cgcctgaata atttcttctt ggccgggacg
     6301 aaatgaatca aatccaaaat atttttttaa ttcatttatt aacattctgt atcgcttcct
     6361 ttctcaatag atagatttga tacaaacgaa attctccata atctaattgt ccatctacat
     6421 tccattcgct aaagcgccac tctcgcacgg aatccttatt tggttccaaa cgttcgattg
     6481 tttctgcaga gagaatccac tcgtatggaa aatcatcaaa aaacaaggcc cactcaataa
     6541 gatgatctgt tactgttcct ctttttaaat gacggattgc catgatctgg tcaattgtct
     6601 gaccattcat aaacatctgc ttggtgatca tcatgctttg attgaaattt tgctgtaata
     6661 atgaatcgat caaagcgtgc caaagcgaat ctggccgttt ttcgatttgt gctaacaaaa
     6721 ggtggataca tctgctttgt tgcagataaa cctctgactc atcattgttt tttgccagct
     6781 gataaggaag cagccctgtt cgctcatttc cagataattg gttagctaaa aaatctgctg
     6841 cttcttctgg tatctccgaa aacagctgca ccaaactctc atacgcagta tcaatcaaaa
     6901 tttcacgtgg caattggctt tcactaagcc atttttttag ttggaaagta taaaaaggac
     6961 ttgtctctgc tggaagatag tcttgaatac ccgttgccag attagaaatt acttgaacag
     7021 caaatttaat gagccgccaa ctagtttccc ctgttctgcc atagcggtca aaacgcaaac
     7081 cagtatgttc tatttgatgt tcggacaaca tatcagcccc tgcaggcgtt agctttgctt
     7141 cattttcatt tatttcgatc caaccttgct gcgcaatttt ttgcaaaatt tgataaaact
     7201 tatcctgctt caattctgga aaactgttat ggataaacaa aagttcgtgg gtaaatccat
     7261 aaataagtac tgaagtcgtt cgttttccca caaggagttg gtaaagagaa ctaatccgca
     7321 gcttgtgtcc tgatgaaaat agcgataaaa taaaaacaat catattattc tccttaagtt
     7381 aggatggtta gaaatgtcac tactatgtaa acttgtacct gaacgctgta ttgcatgcgg
     7441 tctttgtcaa atcgaagcgc cagatatttt cgattatgat gaagaaggaa tcgttttatt
     7501 tgcccaagaa ccagaggctc atcaacagtt cgtgatgccg gaagaagaaa catatgtaaa
     7561 aaaagcgtat caaaaatgtc cagttcgagc gattttatta gaaaataaaa tataaatata
     7621 aacgaaactt taaaaacgta cactaagttc tctaaacttg aaaataagcc gggatctgtt
     7681 tctcttgttc cgtgaaatcc cgacttattt tgaaagattc atttgctatc tatagttaat
     7741 tgactagttt tttcacggac cctataggac ccatttatat tatttcaact ttttcagcca
     7801 gttttccaat gtattgacga tcacagtttg ctggtctgcg ttagaaattt ctgcctctcc
     7861 atctcctttt tgctcaccat agctgccaaa acctccatga tttccgccat tgattgatac
     7921 atactctgtt ctttccggta aatactgctt agcttcttga taagcttcct gattcaatac
     7981 tttatctttt gtcgccgtga tcgataaaac aggaacagct gattctgtca atgttccttt
     8041 tttgtcggga taacttgcca tgaagaatac cccttttaaa tgtttgtctg aatgattctt
     8101 attgacataa cgacttgcca ttacgccacc taatgaatgg ccaccgatca cataatcgga
     8161 atttggaaaa tcttctttga ccttatccgc tttgtttccg cccatcactg ctagatcaag
     8221 tggcattttg acaatagcga ctgaaaagcc gttttttgct aaatcttgcg cccaaatact
     8281 ataactttct ggctctacga gagcaccagg ataaaaaata agtaatggag actttccatg
     8341 attctttttc tctccttcaa aaaataagta atcttctttg ttttttcctt cttttaccaa
     8401 cacttgcgct tctgacgatg gttcgtacgt attttgctgt atatagtaat aaccggctcc
     8461 accgagtaat cctaaaatga tgactaagcc aattagtatt cgtctaaata ttttcacttt
     8521 ctcccacctt ttctatctga gtgacccctt tttcttaatc aagtatgatt ataccttaaa
     8581 aaaataagag agaaatgcaa actttccagc atcaaataaa aaaagacgga atatccattt
     8641 gaatatccgc cctttttgta tgtttatttt tatttagcac ataaacgata cacagcttct
     8701 gcataaattt ccattgcttt atacatacta cttaattccc atcgttcatt cgcctggtgc
     8761 ataaagtcag gagtatcagg gaacatcgca ccgaatgcca cacactggtt catcgtacga
     8821 gcaaaagtag cgccgcctga tacaaacggt tcagtcatgt ctccagtaat atcgcgatat
     8881 gtccccaata gatttttcac taactcactg tctagtggca cgtataatga atcaaggaag
     8941 tcaaattctt cataagtcaa cccatatttt tctgctgttt tagttaattt agctactaaa
     9001 tcatctttct tgaatgtaac agggattctc atatctacac cgatttttgt ttgttctggt
     9061 gtgatttcca aacttgcaaa gttcatcgtt agctcgccgg attgttcatc ttctgttttg
     9121 cccacgacag cttcaccagt tgcattttct tgaactaatt ttcctaagaa attgattggg
     9181 ccaaaatcaa atacttctga aagagcaatt gccagacgag aaatagcatt cactccttgt
     9241 gctgcatctt tcgcgtggat acttttacca agaacgacga tgccttcacc ttgttcttcg
     9301 aaatcgaatt cgtgtttttt caatgcttct tttacttcag aaagcttttc tccagagtaa
     9361 ggagctgcat caggaacaac attcaacgct cctccagctt ttacagaaaa ctcgttcgtt
     9421 cctggtcctg tcaagtaagc ttgcaataag cctttttctg cataaattaa aggaaattca
     9481 gcatctggcg caaatccttg cgtaattcct tcttcttttt cgttatattt ttctaaacaa
     9541 cgccataaat tttcttcatc tgtaccaaag ataaaacgaa tacgtgtctt gaattccacg
     9601 ccagcatcaa tcaaagcttt gacggcatac attgccgcaa tagaaggacc tttgtcatct
     9661 tgggatccgc gaccaactaa ccagccgtct tttatagttg gatcgaatgg ttttgtttcc
     9721 caattatttt cgtcccctgc tggaacaacg tccatatgac acaagatacc gaataattca
     9781 tctccagagc caatctctga ataaccgtaa tagccttctg gatctttaaa tgttctaaat
     9841 cctagctttt cactgatttc caatacttta tccaaagctt caatcacttt tttaccgaag
     9901 ggatgtcctt gtcctgaatc tgcttcatct agtacggaag gaattcggat caattcgctc
     9961 aatgattcta atgcttgttc atgatgttgt tctgtcacaa attttttcat gatattatcc
    10021 tcctaatatg ttaaaaaggg tcatgtcaaa ttcctccgac acaacctctt cttctcaaat
    10081 aatgacgttt taagcctgct ccatcagcaa gcagaatgtt cttaacaaat gatgaaagaa
    10141 aaaacactca ctgaatggag ctattgactt gtccacgtgc ttatcgatag ccgtttatcg
    10201 gtttcttcat tcttatgata gaccttgtac ctgtatttgt ctaggtttag aacattgctg
    10261 ctacacctag gatcacacag atggctacaa ataatatcgc aattaattta ccggtaaatt
    10321 tccaccacac tgaaacatcg aaacgagcaa ttgctacagc acccattacg atagcagatg
    10381 ttggtgtaat taagtttact aaaccagatg cagattgata agcagtgatt actaaatcct
    10441 taccgacgcc ggcaaattca cccataggtc ccatgattcc cattgttgca gcagctaagc
    10501 cggaagttga aggaatcaag aatgacatag ggatatagaa aataaatgta ataatgatga
    10561 atacacttga agataatgaa cttaatcctt gttcgcccca atgtaagatg gtgtctgtga
    10621 ttaatccatt gttcataacc acttggatac cacgagcaac agctactacg attgctacac
    10681 cgatcaaatc agaagcacca gctaaaaatt cagagacaaa ttcagactct ttcatgccat
    10741 aagtgattgc tactacgatg gacattacta agaataacat agtaatttct gggaagtacc
    10801 agtcacctaa tggaagcatg cttttaccta gaaccgtacc tagtaccgga aggtttgtta
    10861 accagtttgt caatgattca aaaatcgtga aattcgcatt caaggttgtc caaggaatca
    10921 aactgatgat catgatacca aaagtaataa agaacaatac attaacatgt ttttgttttt
    10981 tcgtgattac ttcttgacga ccgattgctt caatatcgaa atgcttcata tcttctttac
    11041 gttggttgta tactaatgat tttgttggat ctttttcgat ctttgatgca taacgataca
    11101 catatacaat agaaatagct aacaaaataa cgaacatcaa gaatcgaaga ccaatacctt
    11161 cacccatcga aatcccaaca gcttgtgaag ctacgcctgt tgcaaaagga tttaccgttg
    11221 aagctagaca gcctacttga gaccctacta atacgattgc tacagctact actgtatcaa
    11281 agcctacagc catcatgact gggatcagta aagcatagaa tggaatcgtt tcttctgcca
    11341 ttccgtaagt agagccgcct aatgcaaata acagcatcaa tactggaata agcatttttt
    11401 cttttccttt gaatttcttc acaactgaag caatcccggc atctagtgcc cctgttttgt
    11461 tgataatacc taaaaatcca ccaacaacca aaatgaagaa agagattggg attgcagcag
    11521 gtgtttcttt agttagctcc cccgctggta cacctagcat cccattgatt ggcgccatga
    11581 aaatatccca cagcccttga ggattttgtt gtactcgttc atatgatcca gcgacgatgt
    11641 tgcctgcatc gtctacacta tagtgcccag ctggtataaa ccaagtaagt acagcgatta
    11701 ctgcaataat aataaataag accgtatacg ctgaaggcat ttgaaatttt ttcttttttg
    11761 tttgttccat cttcttcttc ctttcttttc cgttagagac aacaattttg gctctaatgt
    11821 tattttatca aaaagagtat cagaacaaaa ataataacac gcattttgta aaacttttgt
    11881 tcaaaaagtt cgtttttccc tattccctcc tcttttttca ggtttttaaa gctttcttct
    11941 ttttcattgt agcgtttcct tttttttata aacaaacatt ttcgtctttt aaaaggagaa
    12001 gaatttcaca tttgcaaaga attgattgat ttttatgcat aaatcaaaaa aatactcaaa
    12061 acaaacaaat aaaacgttta cattattgca tgtcttgtat ttttttccaa aaaaaaagac
    12121 caagaaattt cttgatcttt ttgatattta tttgactgct gtatgacgtt gttcgaactg
    12181 atgttgtttt cgacttaacc atggaagaag ttgcgcatgc agcactaaaa agacagcact
    12241 cacgatgaat cctttgatca agttgaaagg aacgatccca atcagtacat agtttgctaa
    12301 agacatgccc agcatctgtg ttgcgttcag ccctaaaaac attaagtaaa gaggcgtaat
    12361 aacgaaataa tttgcgatac tcataaaaat agttagagcg atggttcctg taatgacacc
    12421 taaaaatttg tttcttcctt tatgctgttt ctttacacta aagaaataat aaatcgggaa
    12481 tgtaaagatc gttgttgata agaaactagc agtgtctcct accaaattat ccggagaaaa
    12541 acctgtagtc agcagatgta atactgaacg caagaacgct gttgcaactc cggccgctgg
    12601 accgaataag aacatgctga tcaataccga gatatcacta aaatcgatct tcaaaaaact
    12661 aaaagccggc atgatcggaa aagcaaaata ctgtaaaact aatgcgatcg cagcaaacat
    12721 cgctactgca accattttct gtacacgggt gttcttcatt tgaaatcctc ctgttaggac
    12781 tcctcttcag cgaacttttt gacgtttctc tccaaatgaa aaaactctat tttccaggga
    12841 aaatagagtt agagttattt gatcaagcgt cgtcaaataa gctctatctt ctttatccag
    12901 actatactgt cggtatcgga atttcaccga ttcagctatt tttttgcaaa ataggtcgtg
    12961 gactataacc accggtcggg aatttcaccc tgcccctgaa gacgaacctt atataatttt
    13021 tgtttacata ttcattatac atatctattc aaaaaacgca acatctaaca cttataaaaa
    13081 gtaagtgttc attttgagtg gttcagcaaa ctgctgactt cttttttgtt cagttcacgg
    13141 taatccccag gacgtaggcc ttgtaaagtc agatctccgt atttttcacg ttttagtttt
    13201 tgcacaggaa gaccaactgc ttgaagcata tttttcactt gatgattacg tccttcgtgg
    13261 atcgtcaatt ccacgatact gtggtttgtc ttaggatcca ctgataaaat ttggaattta
    13321 gctggagatg tttttcgtcc ttcgatcttt attccttttg tcaaaggtgc aagcatcgat
    13381 tttgtagcga tcccttttac ctttgcaacg taaactttat ccacttcatg ttttggatgt
    13441 gtcaggcgct gagcaaaatc tccatcattt gtcagtagca agatccccga agtatcgtaa
    13501 tccagtcgac ctacaggata gatacgttct tttaccatcg gcaaaagatc cgtaacaact
    13561 tttcttcctt tgtcatctgc aacagcagaa ataactcctt taggtttgta caataaatag
    13621 taaccgtatt cttcttgata aattgggact tcatccactt ccacagtgtc atgtttccca
    13681 acttttatcc ctaattccct tacaacttgt ccgtttactt ttacacgtcc tgcaaggatg
    13741 aattcttctg cttttcgacg agaagtgatg ccagcgtggg caattacttt ctgcaatctc
    13801 tccattacaa ttattctcct ttattcattc catcaaagaa tagatctggt gacacgtctt
    13861 cttcaagtga aatttccatt tcttgtgtat caggcagttc ttctaatgat ttcaaaccaa
    13921 aataatctaa gaagtagcca gttgttccat ataatatcgg ccgacctggt ccttctactc
    13981 tacctttttc ttcaatcagt ttatgggcag ctagacgctg taccgctcct gaaacctgaa
    14041 ctcctcgaat ctgctcgatt tcagctcttg tgattggctg tttataagcg atgattgcta
    14101 aggtttcgac ggcggcttga gataatgtgt ttgatgtccc ttgcgcatat cgcttaagta
    14161 agggagccag ctctttttta gtcgttaata cataatggct ttccgtttca aaaatatgca
    14221 aagctgagtg cgagttttga gtgtaagatt cttttaattc tttcactaaa cttgtgactt
    14281 ctgcttcgct gagatttaac agtccgctta attcttcatt actgatccct tcctcgccaa
    14341 caataaaaag aagggcttca agttcactta atttcgtcat gttcggtatc tcctactgct
    14401 ggataaagca taattgattc atagttttct ttttgttgcg caatgatctg cccttttttc
    14461 atcaattcta gcaaagccat aaaagtagtg acaacttcac ttttagaagg tgactcaaaa
    14521 aaagaatcga aagtacagcc atgcgcattt ctttttatac gctcttcgat aaataccatt
    14581 cgttcttcga tcgttgtgtc atcgggcgtg atcgtttttt ccactttttg agaaaatttc
    14641 ttacgttcta agattttctg catggcacgg atcaaatcga tttttttgac ttgtcctcgc
    14701 ttcaacggct tttcagcatc cgctaaccca tcgatgttca taggttcttt tgtgtaataa
    14761 agacttcgtt cctctgcttt ttctgtcagt ctttcagctg catatttata tttacggtat
    14821 tctaaaagct gagtgaccaa ttcttctcga ggatcttctt cccataattc ttcatcgtct
    14881 gtgccgaatt cttggacagg aagcaatgtc ttgcttttga ttgccatcaa tgtagcagcc
    14941 atcactaagt attctcctgc aacagctagc tcgagtgttt tcattgtatg gatataattc
    15001 atatattgct ccgtgatcgt agcgatcggt atatcatata tatcgatttc catttgctga
    15061 atcagatgaa gcagcagatc gagaggaccc tcgaatacat ctaatttaat attgatctcc
    15121 gtcaactcat tcactctttc ttctttttct ttgctagcca ttttccaatg taaggctctg
    15181 tgatttcagt tgcacggatg gagacatccg ggtacttttc ctgtaattca tctaacattt
    15241 gaaagcccat attctcaccg cgataagacg gattcaaaga aatgtcatgg atgatcagct
    15301 gatcgttcgt tgcccagtaa ataccaataa tcccttgaat gttggttgaa ccttctggcg
    15361 taaagaaaaa aagttcataa tcgtcttcag attcgtacgt atcaatctcc ttcaacaaag
    15421 aagttttacc tgtcagtttc ttatggaaac tcaatagtcc catagcaatt ttacggtttt
    15481 ctttccggta atgtgttaac atcttctcac cttcctatga ctaagccctt ggaaaatgct
    15541 gcttataaac atccgtcatt cttttcttcg tgatatgtgt ataaatctga gtcgtagaga
    15601 tatccgcgtg acctaacaat tcctgtactg tccgcaaatc agctccattt tctagcagat
    15661 gtgtggcaaa gctatgccgc agcgtatgtg gtgttacttc tttgtaaatc ccagcctctc
    15721 gtactaactg cttcagattt ttccagattc cttgccgaga aagacctttc ccatgattat
    15781 tgacaaatac atgcatttca gatgcgtcct ttacaagtaa tggacgtgct tcgtctaagt
    15841 agcgttccag ccattggatc gcataatcac ctaaaggaat gatccgctct ttatctcctt
    15901 ttccaactgt ctgtaccagt ccaagagaaa gatgaagatc tcccaatttc aatccaatca
    15961 attcactgac gcgcatccca gtagcataca tgacttccaa gattgcacga tctcttatcc
    16021 ctaacgtttt tgtcgtatca ggtgcttcaa tcagcttttc tacttctgtc agagtcaatg
    16081 tacttggtaa tttctgtgct ttcttcggcg tatcgatatg ttgcatcgga tcatgatcag
    16141 tgatacgctc ttgccgtaaa aactgatgga atcttctgag acttgagatc atccgagtaa
    16201 cagttgctgt cgcttttttt tcttcatgca acttctccag aaaacggatg actaaaaagc
    16261 gatcgacatc ctgccatgaa gcaattttct gctcctgtag aaaatgcaag tactgttcca
    16321 aatcacgttc atagctttgt cgtgtattgg cggacaatcc ccgttcaata ttcaagtagt
    16381 ggagataatc gttgatttgc tctttcataa taatccccct catgcctctc tatcatatca
    16441 gataccgctt cattctgata gtctttttcc ataatctgag caaagaaaaa gccgtctaga
    16501 cactcagacg gactggtttc tttttt
//
LOCUS       DACEKH010000062        16518 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEKH010000062 DACEKH010000000
VERSION     DACEKH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.065"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            157..1095
                     /locus_tag="IW662_001851"
     CDS             157..1095
                     /locus_tag="IW662_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ0866672.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            1110..1940
                     /locus_tag="IW662_001852"
     CDS             1110..1940
                     /locus_tag="IW662_001852"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0866673.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLLVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            1984..3009
                     /locus_tag="IW662_001853"
     CDS             1984..3009
                     /locus_tag="IW662_001853"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0866674.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNHYFRIYTNPDVVGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            3024..3962
                     /gene="galU"
                     /locus_tag="IW662_001854"
     CDS             3024..3962
                     /gene="galU"
                     /locus_tag="IW662_001854"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0866675.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKIELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            4088..4528
                     /locus_tag="IW662_001855"
     CDS             4088..4528
                     /locus_tag="IW662_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ0866676.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIIRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            4531..5133
                     /locus_tag="IW662_001856"
     CDS             4531..5133
                     /locus_tag="IW662_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0866677.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSAELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(5195..6298)
                     /locus_tag="IW662_001857"
     CDS             complement(5195..6298)
                     /locus_tag="IW662_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0866678.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGADSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVVWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            6508..7509
                     /gene="ccpA"
                     /locus_tag="IW662_001858"
     CDS             6508..7509
                     /gene="ccpA"
                     /locus_tag="IW662_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ0866679.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            7863..8258
                     /locus_tag="IW662_001859"
     CDS             7863..8258
                     /locus_tag="IW662_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866680.1"
                     /translation="MPKKRRKTIVIILTILAIGIGGKFYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGCYGALVTMTNNEGKSVYFDYFFSKQMNEISSY
                     GVENEEIQNEGVTTPKIRVIYSNGEAEEV"
     gene            8255..8509
                     /locus_tag="IW662_001860"
     CDS             8255..8509
                     /locus_tag="IW662_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866681.1"
                     /translation="MTVKDEEYRYISEHVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKDSLDGMQAMAAAPIVHSKLEKNFKNKKIPTNFRVLK"
     gene            8552..8947
                     /locus_tag="IW662_001861"
     CDS             8552..8947
                     /locus_tag="IW662_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866682.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGAYRMFVTMINNHDESVYFTFGFWKERNELGDY
                     GVENKSIQKEGITVNKIRVIFSNNKEEYI"
     gene            8947..10002
                     /locus_tag="IW662_001862"
     CDS             8947..10002
                     /locus_tag="IW662_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ0866683.1"
                     /translation="MMADKQYSVISEAVYWLDPKHRDYDHTYKENSIKKIDSYNFKIL
                     KIKDSLDGMQAMGVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRPG
                     ASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALYV
                     AAEKQWKNVGLNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEVR
                     ISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQEL
                     NNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELEK
                     KWHGKKACGKHVLSPLR"
     gene            9999..10205
                     /locus_tag="IW662_001863"
     CDS             9999..10205
                     /locus_tag="IW662_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866684.1"
                     /translation="MTEQEIIEALASVVATKENLVDSAKEVYLLRINKARRMGEAFDT
                     LVKEIQDKINEIVTRDRELAQQFN"
     gene            10217..10540
                     /locus_tag="IW662_001864"
     CDS             10217..10540
                     /locus_tag="IW662_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866685.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            10533..10799
                     /locus_tag="IW662_001865"
     CDS             10533..10799
                     /locus_tag="IW662_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866686.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNHQ
                     MTAEEAAQEFSNRHVHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(10962..13307)
                     /locus_tag="IW662_001866"
     CDS             complement(10962..13307)
                     /locus_tag="IW662_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0866687.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKVIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            13586..14488
                     /locus_tag="IW662_001867"
     CDS             13586..14488
                     /locus_tag="IW662_001867"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ0866688.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEGGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQAFDPEVFPELLAEEVTL"
     gene            14699..16474
                     /locus_tag="IW662_001868"
     CDS             14699..16474
                     /locus_tag="IW662_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ0866689.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSRRKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT         5451 a         2959 c         3457 g         4651 t
ORIGIN      
        1 tttattctag cttaatttga caaatcatat ataattaaga atgcgttttc tattacattg
       61 gtaattaaat aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat
      121 ggtagagaac actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc
      181 caattagtaa aaaaattatc tttggaaatc gtcacaggcg acgagaaaag tttagaccgc
      241 gtcatcgtga ctggggacat ctcacgccca ggactagagt tgaccggtta ttttaattat
      301 tattctcata atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg
      361 atcccagaag agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt
      421 attatttcaa gaggattgcc ggctccggaa gaaatgatcc aagcagccaa tgaaaatggg
      481 atagctgtgc tgcgttcgcc tatctcaacg tctcggctgc taggtgaatt atctagttat
      541 ttagatgggc gcttagcccc tcgaacaagt gtccacggtg tgttagtcga tgtctacgga
      601 ttaggtgtac taattcaagg ggacagtggg atagggaaaa gtgaaacggc gttggaactg
      661 attaagcgcg gacatcgatt gatcgcagac gatcgagtag atgtctatca gcaagatgaa
      721 ctgacggtaa tcggagaacc tcctaaaatc ctggaacatc tgattgagat tcgagggatc
      781 gggatcatag atgtaatgaa tcttttcggt gcgagtgcgg tacgaggctc tatgcaagtc
      841 cagttagctg tttatctaga agcatgggcg aaagataaga aatacgaccg tttgggttct
      901 gaagacacga ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa
      961 acaggacgaa acgttgcaat catcattgaa gtagccgcga tgaatttccg cgcgcgtaca
     1021 atgggctttg acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac
     1081 tcaggcaatg actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg
     1141 agcttgtagg aattcctatc tactggtatg cattgattat cgtatcagga atcattattg
     1201 ccatgtggct cagcagcaga gaagctgttc gggtaggatt aaaagcagat gacgtgacag
     1261 attttatgtt gtggggattg ccgatttcca ttattggtgc ccgtttgtat tatgttttat
     1321 tcgatcttcc ccaatatata gctgatccaa tccaaatatt caatatccgt tcaggaggat
     1381 tagcgattta cgggggattg attgcaggtg caatcacact gtactttttt accaaatatc
     1441 attttatttc tttgtggacc tttttagata tcgctgcacc aagtgtatta ctggcgcagg
     1501 caatcggacg atggggaaat ttcatgaacc acgaagcgta tggtccagac acgacacgaa
     1561 gtttcttaga aagtctgcat ttaccgcaat tcatcattga taacatgtat atcgaaggga
     1621 tctatcgaca accaaccttt ctttatgaat cactttggag tttgattgga tttattctgc
     1681 ttttggtttt gcggaagaaa ccgcatcttt tgaaaaaagg agaagtcctt ttatgttatc
     1741 taatgtggta tagtttcgga cgtttcttta tcgaaggatt acgcacagac agcctttact
     1801 ttttaggtgt gatccgcatt tctcaattat tgtctgctgt tttacttgtc ggcacattga
     1861 ttctgtttat ttggcgtaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa
     1921 aaaatcaaca aattatttga tttttagagc gttaaaaatg aaaaaaattt caggagtggt
     1981 aaaatgaatt atagagtagc ggttttagga ccaggctctt gggggactgc tttagcacaa
     2041 gtcctcgctg aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa
     2101 gaaatcaaca cccaacacac caatcaacgt tatttaccag atcatcatct acccgattcc
     2161 atccgagcat tcaccgatat ggaagatgca gtaaaagatg cagatgcggt actattcgtt
     2221 atcccgacga aagcgatacg ttctgttgca aaagaactag ttgccaaatt gaatacgaag
     2281 ccagtgatca tccacgcaag taagggatta gaacagggaa cgcataaaag aatctctgaa
     2341 atcttgttag aggaaatccc gacagaaaaa agaaaagctg ttgctgttct ttctgggcca
     2401 agccatgcgg aagaagtagc aaagcatgac attacgacga tcacagcggc aagcacagat
     2461 gcaaaagcag caaaatatgt acaagagctg ttcatgaacc attattttcg aatctatact
     2521 aaccctgatg tggttggtgt agagatgggg gcagctttga aaaatattat tgccattgga
     2581 gctggtgcta ttcacggctt agggtttggc gataatgcaa aagcggcaat catgacgcgc
     2641 ggattagctg agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatt
     2701 ggcttaagcg gagtaggcga tctagtcgtt acttgcacaa gtgttcattc cagaaactgg
     2761 cgcgcaggca acttgttagg acaaggacag cctctagaag aggtgctagc caatatggga
     2821 atggtcgttg aaggtgtgaa tactactcaa gcagcgatgg aactagccca aatgatggaa
     2881 atcgaaatgc ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa
     2941 acagccgcaa aagaaatcat gttacgtgat gggaaaaaag aaaatgaatt ctcattaaca
     3001 attttttgaa ggagcgaatg tatatgaaag taaaaaaagc agttattcca gcagcaggat
     3061 taggaacaag atttttacca gcaacaaagg cgatggcaaa ggaaatgttg ccaatcgtgg
     3121 acaaaccaac gatccaattc atcgttgaag aagcgttgaa atcaggaatc gaagacatct
     3181 tgatcgttac aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatatcgaat
     3241 tagaaacaaa cctaagtgaa aaaggaaaaa tcgaattatt gaaattagta gaagaaacga
     3301 cagatgtcaa cttgcatttc atccgtcaat ctcatccaaa ggggttagga cacgccgttc
     3361 ttcaagcaag agcctttatt ggaaatgaac cgtttgtcgt tatgctaggc gatgatatca
     3421 tggaagacga agtaccattg acacagcaat tgatcgatga ctatgaacgt acacacgctt
     3481 ctacgattgc cgttatgcaa gttcctcacg atgaaacttc aaaatacgga attatcaacc
     3541 caggggaagt actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc
     3601 caagcaaagc accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct
     3661 ttgatgtatt atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa
     3721 tcgatacgtt gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg
     3781 acgttggtga caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag
     3841 aagtaggggc acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag
     3901 gaagcaagat cgatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat
     3961 aatcattttt ttctaacgag cttcagctgc taattgaata aaaagtagtt gaagctcgtt
     4021 tttatttgtt taaaaaacac gaaaatgcta tccttataga aaagagagac aagaagggag
     4081 tttgatcatg tcaggaggag aaattgcagg attgatcgca gcaatcgcct ttgcgatact
     4141 tgtcgttttc atcattcgcg tattgttgca agtttccaaa actttagaaa aagtagacca
     4201 gactgtcgca gaagcaaata cgacaattga gatcgtcaca aaagacgtag atcttttatc
     4261 tagacaagta gaaggcttgc tggtcaaaag taatgagctg ctcttggata tcaatggaaa
     4321 agtcgcaacg atcgatccat tgttcacagc agttgctgat ttaagtgaaa gtgtatcgga
     4381 acttaatcat tctagtaaaa acatcgtgac gaaagtcggc ggaatcggaa aaacgacagc
     4441 taaagcgact gttgccggaa aagtcggtgg aacagcgatg aaatttttta aaaacaaaaa
     4501 acatactgaa acgacaggag gaaaataaga atggctaaaa aaggtggatt tttattaggt
     4561 gcagtagttg gaggtacagc cgcagcaatc gccgcattat tgttagcacc caaatctggt
     4621 aaagaacttc gggatgaatt ggctgctcaa gcagatgatt tgatggacat ggcttcagat
     4681 tacacagata tggcaaaaga aaaatcagct gagttaacag gcattgcaaa agaaaaagcg
     4741 caagatcttt ctcaacaagc aggggatcta gcaggtaatg ttaagcaaaa ggtaagtgaa
     4801 tcagcagagg aagcaggaag tgaatcagat gatatcttgt taaacctcaa agaacaatca
     4861 gctgatttat ctaatcgttt caaaaaaaca gcagaagatc tgaaaaaacc agcagacgaa
     4921 ttaggtagta ttgctaaaga tacttcagaa gatatcatga tcgatgtaaa agatgctgca
     4981 gataaagcag gagagacggt caatgaagga atgtcagaag caaaaccagt tgcagatgac
     5041 acaaaagaaa tgttgaaaat ggcaaaagaa gatgcaaaag aagctaaaaa agaaatgaaa
     5101 gatacaatga cagattcgac taccaatcac taagaatact catttctaga ataaaaaggt
     5161 gagagcggac cagctcccac cttttttatt ttcattactc taaaacaagt aattcttttg
     5221 gtgtagtcgt gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta
     5281 cccctacttt ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcga
     5341 tcactaagtc gtttccttct acaagagaag ggaattcgtg gacggttgtc ccaattccgt
     5401 gacctaaacg gtgattgaaa tattcgccgt agccatagct gttgattacg tttcgtgcga
     5461 tttgatcaag ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg
     5521 cttcaagagt gatgttgtag attttttctt ggaattctgt tggtttttga taagcaactg
     5581 tacgagtagc atcactgcaa tagccattcc aaacaacgcc aagatcgaat aacactagtt
     5641 catttggtga aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg
     5701 caaggactaa ggtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga
     5761 tttctgcgat aattgcttgt tcagtcacgc cttcttttac tgttttgaag ccaatttcaa
     5821 aggcaacatc ggcccattta ccagcttcga tcagtttgtc tttttcttct accgtcttga
     5881 tcagttgcag acgctggata atagaagtta gatcagtgta agaatctgca cctgtcaaga
     5941 tatcagagat agcatcgaag cgatcgacag tcagagcatt cttttcaatc cctacattac
     6001 caggttgccc atagcgtgcg cgaagctctt ttccgatgat ctcccaaggg ttttcgctgt
     6061 ctaagtagcc acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag
     6121 caggagtgaa aaggaaagga tcttgttcaa gagggataaa aagagctagt acacgctcgt
     6181 gaggatcact ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca
     6241 attctacttg gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt
     6301 ggcaacctac tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag
     6361 accaacgttc ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt
     6421 gcaattgttg agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac
     6481 atataaaata tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta
     6541 gcgagagagg caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac
     6601 gtcaaaccag caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca
     6661 aatgcagtcg cacgtggctt ggcaagtaag aaaacaacaa ctgttggggt aatcattcct
     6721 gatgtcagca atatgttctt tgcctcttta gcacgtggta tcgacgatgt agcaacaatg
     6781 tacaaataca atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta
     6841 ttgaataatt tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca
     6901 gacgaaatcc gtggagaatt ttcacgttca aaaacacctg ttgtactagc tggttccatt
     6961 gatccagatg aacaagtagg tagcgtaaac attgattatg tatcagcaac aaaagatgca
     7021 gtaaataaat tagcaaaaag cggcaataaa aaaattgctt ttgtcagcgg tgcgctgatt
     7081 gatcctatca atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat
     7141 ctaccatata gcgaaggatt aatctttgag gcacaataca actttaaaga tggtctgtct
     7201 ctagttgacc ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta
     7261 gccattggta tcttggatgg tttattagac cgtggcgtaa aagtaccaga agaattcgaa
     7321 atcatcacaa gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc
     7381 acacaaccat tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat
     7441 aaagaagaag tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca
     7501 actaaataat aaaagatcaa tcatttagag gttgttccaa aagtggccaa cttctggaac
     7561 aacgtttatt cgagaaaagg ggttatgaca aaattttgtc ataacccctt ttttttgtga
     7621 ctttaaaaaa agagaaaatg gcggaaacaa accttacgtc catcgtatac gacacaatct
     7681 gtgcgtagat gctacatttt gttcactact atcaaaatta ctttaaaatg aaaacgtgat
     7741 aaaaaagaaa gtagaaaagt agaaaagtaa ctctaaaaaa tccaaaaaaa tccaggctcg
     7801 attgcgtact tatcttaaag gagaaacaat ccatcataca taacccgaaa ggagacttct
     7861 atttgcctaa gaaaagacga aagaccatag tcatcatttt gaccattctt gctataggaa
     7921 tcggaggaaa attttacatg gacaaaagag aagcacaaag acagaaagat ttactcgcag
     7981 tagaaaaaca atccgtcaaa gttctgaaaa atacctttgc agatatcagg gaagtgaagg
     8041 tagaagaatt taaaaaaaac cctgttactg gatgttatgg tgcacttgtg acaatgacaa
     8101 ataatgaagg gaaatctgtt tattttgatt attttttttc aaaacaaatg aatgaaatta
     8161 gcagttatgg tgtagaaaat gaagaaatac aaaacgaagg ggttactact cctaaaataa
     8221 gagttattta ttctaatgga gaggcggagg aagtgtgaca gtaaaagacg aagagtatag
     8281 atatataagt gaacatgttt attggctaga tccaaaacat cctcaatata gtaaaagtta
     8341 caaagaaggc gccattaaaa actttggaga aacaaaattc caaatcctaa aaatcaaaga
     8401 ctcattggat ggcatgcagg cgatggctgc agcgccaatc gtccatagca aattggagaa
     8461 aaatttcaaa aataaaaaaa ttcctacaaa ctttcgcgta cttaaataat gaaggtatca
     8521 atacttacat aaaaagaaag gaggcacgtt cttgtctcgg aaaataagaa aactcagctt
     8581 agccatttta ttaatcgtca tattaggaat cggagggaaa gtctatatgg acaaaagaga
     8641 agcacaaaga cagaaagatt tactcgcagt agaaaaacag tcagtcaaag ttttgaaaaa
     8701 tacttttgcg gatatcaagg aagtgaagat tgaaaaatct gctcgaaatg aaatgacggg
     8761 agcatatcgt atgtttgtga caatgataaa taatcacgat gagtctgttt attttacgtt
     8821 tggattttgg aaagaacgaa atgaattagg agattatggc gtagaaaata aaagtataca
     8881 aaaagagggg attacagtaa ataaaatcag agtaattttt tctaataata aggaggaata
     8941 tatttaatga tggcagacaa acaatacagt gttattagtg aggctgttta ctggctagat
     9001 cctaaacaca gagactatga tcatacttat aaagaaaatt ctattaaaaa aattgatagt
     9061 tataatttta aaatcctaaa aatcaaagac tcattggacg gcatgcaggc gatgggtgta
     9121 gcgccaatca agaacggtaa agtggatctt tctcaagtag tgattgcttt tgcaggaacg
     9181 aacttaagtg attggaaaga tttggaaacg gatgcaagga gtatcatgaa tctctatggg
     9241 aatagaccag gagcaagttt ttctgaaaaa gcaacaacaa acaagatttt atccgttagt
     9301 ggattgaaaa cagcggggca aatcaattca gcgattgcat ttgctaatga agtaaaaaag
     9361 gaatatggag aagctgctat ttctactacc ggccattcat taggtggatt cttagcatta
     9421 tatgtagcag cagagaaaca atggaaaaat gtcggcttaa acgggccgga cccgtatgat
     9481 ctcttatcac cagaagcaaa aaaatgggtg gaagaaaatc ccggtatgct caccaattac
     9541 cgaaatcacg ccgattttat cggtaatttt ggtggtaatg gcacaggtgc tgaagttcgg
     9601 atcagtatgg aaacagcatt cttgaatctg cttgcttccc acagtttaac ctcttggaag
     9661 tttgatgaaa atggaaagct gattattcct gaaaatgaaa ataatgttaa agctcgaaaa
     9721 caacagaaag aaaaccaagc gacccagttg ttttcctatc agttgcaaga actgaacaac
     9781 atacgtaaaa aactacgatt aagtggcggc cggctgacag ttaatgaaca aatttactta
     9841 gatgacacac aagctaggtt agtagtggaa aagttatcag caaaaatgaa aagcgctatg
     9901 gaagaagtta tccgcatcaa tcaaagagga atttttgagc tagagaaaaa atggcatggc
     9961 aagaaggctt gcggcaagca cgtgttgtcg ccccttcgtt gactgaacag gaaatcatag
    10021 aagcattagc tagtgttgtc gccacaaaag agaacttagt cgattctgcg aaagaagtct
    10081 atctgctgcg aatcaataag gcaagacgta tgggggaggc ctttgataca ttggtgaagg
    10141 agatccaaga caaaatcaat gaaatcgtga cacgagatcg agaattagca cagcaattca
    10201 attaaaggag aagtagatga aactagaaga attggaaaga gattatcgaa gtgaacgaag
    10261 acaattagaa gctcaagaag atgaattgat ccactttaag aatcaaggat tacgattgat
    10321 ggagcaagag gagcagcgac tgcatcatgt attaaagaac ttagctttag ataatgagcc
    10381 tctgtttcaa gcaagacgaa gttattcgaa ttatcaggag cattatcaac aagccctaca
    10441 agtagaacga cgaaaactct atcagcagca agacgaacta gaacgttcct atcgtcaaaa
    10501 aatcagacaa ataactatgg agaaatcatc ttatgaatga atttttaatg caattactaa
    10561 aagaattaga gaaaaatcta catcgatcaa atgtacgttc agttggtatg gggatatctc
    10621 agcaaatttg ggcagaaaaa cgattgacag atcaaaacca ccaaatgact gcagaagaag
    10681 ctgctcaaga attttctaac cgacatgtac acttgaaaga atcagtaaaa gggcaatttg
    10741 cagaaaaact aaccgaatta ttccaaagac aagaaaagaa attaaaacag tttcgttagt
    10801 aaagttatat agaaaaattt tgtgaaaatg gcttttcaca tttttacata aatttgtctt
    10861 aatatcgtag atgcaagctt ttgaacacca tttattcgga aaaagggagc tgtgacaaga
    10921 tttttgtcgc agctcccttt aattctgatt aatctattag atcaatcatc tttatcgtct
    10981 gattctttgg tctctggatt cgctctagca taagctgaag cggtgttcca gtaatctgta
    11041 tagttttcat cagtaccgcc aacgccgaat ttaaaggcgc ttttcttcgg accatttttt
    11101 gcccaaagtg attccactgt ttttcctggc gtttggatag attttttatt gacagtaact
    11161 tttccagctg tttgtcctgt gaacccagat acttttttct tcatcacatc agatgataag
    11221 tcaaatttct catcagtgcc gaaaatctca gggtttgctt ggtaagcttg attgattaga
    11281 ttggcaagat aattagccga acgtatcctt gcttggctgt ccattccagt attgtcatcg
    11341 tgtcctgccc agctactgat agtgatagac ggagtagaga cgatcagcca tgaatctctg
    11401 tattcgttgg tagaaccagt ttttccgacc caatctgctt ttcctagatt tccgtttaag
    11461 cttgaaatga catctttgaa tggtgtagtg attttttcat tgatcacact gcgcatcatg
    11521 tcattcatga tcgaggccgt tgcttcggaa taaatacgga cagaattact ttcatgttta
    11581 taaatcacat tacctgcatt atctgtgatg gaatcaatga tatagccttc ttcgtagacg
    11641 ccaccattag ctaaggcctg gaatccattt gtctgttgaa gcgtcgttac atctactgtc
    11701 cctaaaggag cagattcaac gccccaatta tcatttgctg ggtagttcat cttcgccaag
    11761 tagtgctcgt aagcatattg tttcgatcca cccgaattca atacgtcttg gtataagtga
    11821 taagctggga tattgttgga ccattccagt gactcacgga cggtttggaa ggtgttcgac
    11881 ccttcattgg tcgcattgac gattttctcg ccagcatttt ctccttcttg ccaagtcgtt
    11941 gcataatctg atacacggga ttctgatccg acaagtccca tatcgattgc aggaccgtaa
    12001 acaagaaccg gtttgatgga tgaaccagct tgacgttctg tatcaaaggc gtggttattt
    12061 tgattctggc tgtaattccg gccacccacg aatccataga tacgtccagt cctattgtcc
    12121 atcaatacat ttccagtctc cacttggcta ccagcaccat catctaacat atagccgtaa
    12181 ttttctacgc cggtttgcaa tgcagcatag atatctttat cgatcgtcga atggatcgta
    12241 tatcctttgt tttggatcgt ttgctgtgct ttttgataat aagcttcact gaccgatgaa
    12301 tctgacatat cagcattatc tttttctgcc aattgttgtg caataatcct tgttgcttcg
    12361 ttcatcacgg tataatacaa gaattctctg tcagtctgtt ccgcgatttg ttgtgggaga
    12421 aagtcttttg taagatcata ggctttcgct tcttcgtatt gttctttcgt aatttgtcct
    12481 tcacggtaca tactgaataa aacaaaatct tttctggcaa gaccggcaga aagatcttct
    12541 tttagcgcgc ctgtattcgt gtagggacta tacgtgatag ggctttgagg caaaccagcg
    12601 atataagcag cttgtggcag agtcacatct tttgcatcta caccaaagat tcctcttgca
    12661 gcttcctgga ctccagcaat attttgcccc ttattattcc gaccaaatgg cgagacattc
    12721 aaatacgtag caatgatctc atcttttgaa aagtattttt ctacttgtgc agaaagaacg
    12781 atctcgtttg ctttacgttt gaatgttgtt tcatcggtta ggatctgctg tttgactaat
    12841 tgctgtgtca aagtggatcc tccagacgag ccgatgccag tagcttctga aactaatgct
    12901 cgtaaaacag ctttagggac ataaccttgg tgtttatcga agtattcatc ttctgtactg
    12961 ataatcgcgg ttttcaataa aggcgaaatc ttatcagatg aaatagtcgt ccgcatcaga
    13021 tctgtctgga tttttgatat ctctgtatta tctgcataaa caatctttga cgtttccgta
    13081 atgtttccta gttctgtctg cagttctttt ttagtcggca attgtgtgtc ttcgacaaga
    13141 taggcaaagt agccagcacc tattccgata cctagagctg cgaacaacaa aagaagaaca
    13201 gtaataaaaa cagtcaatga ttgaaacact ctaaaaataa ccttaggaac aaaccatttt
    13261 tctttttttc ttctattctg tttttttcga gtatttttgc gtggcaaaag ggtcacctca
    13321 actaatggtt ttatttcgct aattatagca aataataaga caaaaaaaca gacaaggctg
    13381 tttttgacag gaatttcttt ttcgaatgag ataaaacgtg aggaaatgat cttttttatg
    13441 aaagagttct taagcttttc tttagagaaa gtttcagtta ctatggagaa cagggcaaag
    13501 aaaaacaaac cattttcttt gtacatcatc ttaccaatat ataggaattg ttcgaaacaa
    13561 ttgcctttca aggaggaatc catccatgtt attaggctca cacgtcagca tgagcggaaa
    13621 gaaaatgctg ttaggcgcgg cagaagaagc agccagttac ggatcttcga cctttatgat
    13681 ctatacaggc gctcctcaaa atactagaag aaaagctatc gaagagatga atattgatgc
    13741 cggaaaagca tttatggcag aaaacggact aacaaatatc gtcgttcatg cgccctatat
    13801 cattaatttg ggaaatacga tcaaaccaga aatgtttcca tttgctgtac aatttctgag
    13861 agaagagatt gttcgtgcgg aagctttagg tgctactcaa atcacccttc acccaggcgc
    13921 tcatgtagga gcaggggcag aaggtgggat tgctagaatt attgagggtt tgaatgaagt
    13981 tttgacaaaa gaacagacgg cacagatcgc tttggagacg atggcaggga aaggtacgga
    14041 aatcggtcga acatttgagg aactagcgca aatcatcgat ggagtaaaat tgaatgaaaa
    14101 attatccgtt acttttgata catgtcatac gaatgatgcc ggatataata taaaagaaga
    14161 tttctcaggt gtgatggaag agtttgataa ggtcatcgga ttagatcggc ttaaagtact
    14221 ccatatcaac gattctaaaa atccacaagg ttctcataaa gatcggcatg ccaatatcgg
    14281 ttttggaaca attggatttg acacattgaa caaaatcgtc catgatccaa tgtttgacaa
    14341 catttcgaaa atcttagaaa ctccatatgt cggtaaagat aaaaagaaag cttacgcacc
    14401 atatggaaaa gaaattgcca tgttcaaagc gcaagcattc gatcccgaag tgtttcctga
    14461 gttattagca gaagaagtca cactttaagt tttctatctg acagcaacaa aaaaggattg
    14521 ggcagtctgc cttgtccttt tttgtttaag aaacaaagga gtgctttttg cagtcgtttt
    14581 cgccatgctc ctttcgcccc ctctttttaa attcagacaa acagggtata ctgatagaaa
    14641 agtaaaaaac gaaaagattc acaagcaagt agcaaaacgt gattgaaagg acgaaaagat
    14701 gctgaacact ttaaaaccct tgatcaacca actaaaaaag aaacatacat actttattct
    14761 tggcatagcc gtattattta caattattcg gttgtattta tatagtaaag caatctatgg
    14821 aacagatatg tcggcaacct atgatgatca gctgctgatt cactatgctc aaaatatttt
    14881 ggatggtaaa tggctagggg agtacacgac tacgacactt tcaaaaggga tctcctacag
    14941 cttgttttta gtacttgcac ataaactgca tctttcttat gcagttttgt tcggcatgtt
    15001 cagtattctt gccagcatat tggttgcact agcgtttaaa ccgatcgtga agaacaagta
    15061 tcttctttta agtatctatt tttatttttt gttttctccg attagtttta cacatgagta
    15121 ttccactcga acgtatcgta atgctctcgt gatcccggct gttgagatca ttatcgcttg
    15181 tttactagct atttattacc gtaaatatag ttcccgcaga aaattgctgc cgtggttttt
    15241 agggttagct gtttcttttc ctttcttttg gttcattcgc gaagattcca tctggcttct
    15301 tccttttact gtagtcgcgt taggcatcag tttcgttcag atcttgctag gacaagactt
    15361 ttctttcaaa gaagcaaaga agttaaaaga acgaccattt ctcctttcga aacaagaatg
    15421 gctgaaactt tcattgtttt tactccctct agtcggtttc tccatcgtct atcagtggat
    15481 cgaatataaa aacgagacaa catatggtat tgccaccatc aatgaccgtt cgagtggtga
    15541 atttggacgt ttgatgaaac agctgatccg aatagatgac gaaacgaact taaataaaga
    15601 aaacagtgag atatgggtct cgcatgctgc attaaataaa gcattagacg catctcctac
    15661 atttcatact gtttctactc gtattgacga attgtatacg actcatgcgt ggacccaagg
    15721 cggaaaagta aaagaattac caggagatat cattttttgg gcattgcgag atgtggtaag
    15781 cgaatctggt tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa
    15841 tcaagaatta gaagaagcct atcaaaaggg aactatcaaa aagaaaaaag aattctatct
    15901 gatgggatca ggggatggaa agacgatgaa tgatctgcca atcgtatttg cttttctgaa
    15961 atcaggatat gcctatgcat ggcgatacaa agatttccat caaggaggga attatagttt
    16021 cgggacgcaa gaacaagtcg aacaggccca agaattattg aatcttccac taatggataa
    16081 ttggatcgat agcagtgatc cgagaacacc tgatttccag ttgacaaaaa cagcaaaagt
    16141 cgcgaatgtc atgataaaaa tttaccaagt tctggccatt cccgtgctga ttgcttcaat
    16201 agcagggata ttacttatga tactaggaac cgtatttgca aaagctgatc gtgcctatta
    16261 tagttcctct ttgattgtag tcaccggatt aatactgaca caattggttt ttcttttcgg
    16321 tgtctcgtgg ttttgttctt atgcgccaga aaaaaaggat tattttttaa gtgtctacac
    16381 tggcgcaggc gtgccattaa cacaattcat cgtagtaaca agcatgctag ttttcagtaa
    16441 attgaatctg aaaaaagctt tatcttctaa ataaaaaggg aactgtgaca aaagtcttgt
    16501 cgcagttcct ttttccga
//
LOCUS       DACEKH010000063        16241 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEKH010000063 DACEKH010000000
VERSION     DACEKH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.066"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            238..906
                     /locus_tag="IW662_001869"
     CDS             238..906
                     /locus_tag="IW662_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ0866690.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1106..1492)
                     /locus_tag="IW662_001870"
     CDS             complement(1106..1492)
                     /locus_tag="IW662_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866691.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1464..2846)
                     /locus_tag="IW662_001871"
     CDS             complement(1464..2846)
                     /locus_tag="IW662_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0866692.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3002..4189)
                     /locus_tag="IW662_001872"
     CDS             complement(3002..4189)
                     /locus_tag="IW662_001872"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ0866693.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4196..5227)
                     /locus_tag="IW662_001873"
     CDS             complement(4196..5227)
                     /locus_tag="IW662_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0866694.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6693)
                     /locus_tag="IW662_001874"
     CDS             complement(5230..6693)
                     /locus_tag="IW662_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0866695.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPIKKGS"
     gene            6868..7773
                     /locus_tag="IW662_001875"
     CDS             6868..7773
                     /locus_tag="IW662_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0866696.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7864..8541
                     /locus_tag="IW662_001876"
     CDS             7864..8541
                     /locus_tag="IW662_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0866697.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8822..9718
                     /locus_tag="IW662_001877"
     CDS             8822..9718
                     /locus_tag="IW662_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0866698.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9773..11143)
                     /locus_tag="IW662_001878"
     CDS             complement(9773..11143)
                     /locus_tag="IW662_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0866699.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11381..12127
                     /locus_tag="IW662_001879"
     CDS             11381..12127
                     /locus_tag="IW662_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866700.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12124..12963
                     /locus_tag="IW662_001880"
     CDS             12124..12963
                     /locus_tag="IW662_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0866701.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12964..13914
                     /locus_tag="IW662_001881"
     CDS             12964..13914
                     /locus_tag="IW662_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0866702.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14055..14807
                     /locus_tag="IW662_001882"
     CDS             14055..14807
                     /locus_tag="IW662_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866703.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14826..15977
                     /gene="queG"
                     /locus_tag="IW662_001883"
     CDS             14826..15977
                     /gene="queG"
                     /locus_tag="IW662_001883"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ0866704.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5274 a         3022 c         3253 g         4692 t
ORIGIN      
        1 ctggcaaagt ctcgtccaat ttcgtaaaac aaccgtaacg gttgtgtaaa aatatcattt
       61 tagagcataa aaaaaaggag tggatttctc cactccaaaa aagtctaact tttgggggtc
      121 actacagtgt cacagtcctt tttttagtaa aaggatacat ttcttccttg tatattccat
      181 gcgtgttcgt gagaaagcat atataattta attaacagat agttaggggg agaaaaaatg
      241 attttttcaa ttacaatgaa actggtgttc gggttgatcg gtttattatt agtggtacgt
      301 ttgcttggga agaaatccat gtcagaaatc acaccgtttg atttagtcta tacgcttgtt
      361 ttaggtggga tcttagaaga atcaacctat gatgacaatg tccatgtagg gcatgtccta
      421 tttgccattg cgctttgggc agtaatgatt tatggtatcg aacgtatcgt ccaaaaaaat
      481 gaaaaggtca atcgttgggt caaaggcgag ccatctgttc taatcaaaga cggcgtcatc
      541 aacatgacag agttgacaaa taaccatatc gagatggagc agttgcgagc aatcttaaga
      601 cagcaagaat gcttttttct tgaaaatgcc aaacatgtta tcctagagaa tgccggtcaa
      661 atgagtgtat tgaaaaaatc agatgaagac aaagctttat cgatcctgct tgtagatgaa
      721 gggcaaattc agcataaagt gcttcagtcc aatcaattga ctgaagcatg gcttatggag
      781 aacttgaaga aagaaggcta tgcagatgta aaacagctga tttatgttga atggtcagaa
      841 gaaaaagggc tctacatact aaataaagaa gacgtcatgc atgaatctta ccgaatagat
      901 ggatagatag acagaggttg tgaaatcagc gccttggctt gttactcaaa actaagcgac
      961 tttttcacaa cctctgatat ctaatatacg ttgttccaga agttgcttct tcgaaaaata
     1021 agccaaaatt ccacaaaaat ttaagaagca attttcgaaa aatcctttct tattttttga
     1081 agttgatcac ttctgtcaca acctctcatg tcacaaacca tttttttgtt tcctttaact
     1141 caatataccg tacgtcttcc cacaatatcg gctgatggtc aatcataaag taatcttcat
     1201 aggcttcacc aatgaagtgc ccttcgacct catccaagag tcttccaaat tcatctttta
     1261 aattgagctg aatgattact gctttacgag ataaaaaggc ttctgtcagt atactatcga
     1321 tttcttgcaa tgtcatttgt tgtttaggaa gaggctgttt gcgtgcttct tcttcatttt
     1381 ttttgatacc gttgactagt tcttccatcg caaaagcagt cccccatttt aatccaaatg
     1441 gccgatctcg atactcgtta tagtcaatga atttttttgc tgtccgtctc atcttttcca
     1501 ccttctaatc cactcatacc acctgcatgc ccgccaacga gagaactacg tgcgatggcg
     1561 cgtcctcctt gcaagagact atgcgcatgg atgattgatt gaaagccgta tttttttcta
     1621 atcgtatcga caacaaaatc aactttcgct tcatttatct gcttttcagg atcttcaaaa
     1681 aggttaagct gtaaacctgt cgcgtatacg aggtcggaac agttcacgcc tacacgccga
     1741 atatcctggt agtggtaatg ctggtggaaa atggtcaata aagcatctgc tagatctctg
     1801 ctagcatttg tcgcttcgat tttcatttgc tggtgaaacc cggtcttgcc atttggatca
     1861 agatagccta aagagtatcc tacccataag ctgactactt gtgtttttgc tgcagatcgt
     1921 ctcaagcgag tgccgacttg gtcggccatt tctttgataa cgatctcgat ttcttctcgt
     1981 cttgtataat ctctatctag gatttgacta tttccgatac tttttgattt tggtgtatat
     2041 tcttgtccca aaaaacttcg atcgatgccc caagcatggg cataaagctg agtaccgatg
     2101 acccctagct tgcttttcaa gtgataataa tccgcgtgag ctaaatcata aacagaatgt
     2161 atacccattt gtttcagacg ttttgctgtt cgtttgccga ttccccaaaa gtcagtgata
     2221 tctgagattt tccataactt ttctggtaca tcttcataac gccattcagc aaacatttgc
     2281 tttccgtctt ttgagccatt gtctaaggct agtttagcaa gtagcgggtt atcgccaatt
     2341 cctatagtcg tgtagattcc ggtctgctca aacacatctt tatggatcag tttagccatg
     2401 tcgtacgcat ttgttttacc aaagagtgcc atgctatttg ttacatcaag gaagctttca
     2461 tcgacggaat agacatgatg gttcgcttcg tctgcatatt ttttatagat agaattgatt
     2521 tctctgttct tttccatata ataggccatc cttggggcaa caatatataa atcacttgga
     2581 taggggaagg gaagatctct ggcacggctg atattggtga tgccgtaagc tttttttgct
     2641 gcaggactgg aagccaagat taatccgcta ccccgtgttt ctgtcgaatc ggacggatag
     2701 gacatgacta ctaatttagt tgtaagggga tcaagatcct tttcaacgca ctctacggag
     2761 gcgtaaaaag atttgcagtc aatacataaa atatctcttg aaggttcttt ttcataaaca
     2821 aataccggat tttgattcgc ccacataaca atcacctctt caacacaatt agaacacacg
     2881 ttctctttcc gtgtcaataa aaaacagcaa ttttcaaata cgagcaaaag gctcacattt
     2941 ggaaattgct gagaggttat ttttatctat ttgtttttag ctgagatcgt tgattttttt
     3001 atcaaacatg aacagcttct ggatcgatct ttgttgtccc atttccaatg tgtgcaacat
     3061 aaaattccgg agcatagccg acaactttca tgtattcatc atagacattg tttttgaagg
     3121 caggtatatt ttcttctttg acaagagcaa tcgcacagcc accaaatcca gctcctgtca
     3181 ttcgtgcacc taagacacct tcttgttttt gagcagcatc tactagtgta tctaattcga
     3241 tcccagttac ttcataatca tagcgaagag agtggtgaga atcatttaac agttgaccaa
     3301 atgcttctaa gttcccagct tctaattcag cttttgcctt caaagtccgc tgattttcag
     3361 aaacggcatg gcgagctctg cgtatcaatg tatcatcgcc gatttggtca atagttgatt
     3421 caaattcttc ttcatctaag tctcctaaag aagagatagg caaagttttt tgcaaacgtg
     3481 ccaaggcttc ttcgcattcg ctacggcgtt cattgtattt tgaatctgcc aattcccgcc
     3541 gcttattcgt attcatgatg acgattgcat aaccatctag tttcactgga accatttcgt
     3601 atttcagcgt atttgtatct aataagattg catgatctag ctcaccgaag ccgatggcaa
     3661 actgatccat gatacctgaa ttgacaccga tgaattcatt ttctactttt ttacctgttt
     3721 ggactagttc caagcgatct gtttttaaat caaacagatc ttctaataca acacctacta
     3781 ataattctaa tgaagcgcta ctggaaagtc ctgcaccatt agggattgtt ccttccacta
     3841 ataactcaaa cccatgatcg attgagtgtc cagcttcttt taatttaagg accattcctt
     3901 ttacgtaatt tgcccagttt gcttttttat catattctag ctcatctaaa gtaaaactaa
     3961 tgacaccgat atcgtcaaag tttgtggaat aaactaatat tttattatcg tctcgaggtg
     4021 ctgctacacc ttgcgttcca taagtgatgg aagcggggaa gacatggcct ccattgtaat
     4081 ctgtgtgttc accaatcaaa ttgatgcgtc ctggtgagaa gtattgtgta gtttttttag
     4141 gtccgaatgt ttcggcaaat tttgtgttta agcttctttt gatttccatg atgagttatt
     4201 cctttcttgt tttgattttg aattctgtca ctgtttcaaa tactgatccg gcttttaaat
     4261 gaatcgaacc aaatgctgag aagcgctctg cacctggagc tgtttgagtc tcaaatgtga
     4321 tgccaccgtg atgtgccagt tttcggttgc gcatttctgg tgtttctgtg ccgaaatttg
     4381 cagtgaaaat aaccacacta gaagcatctg tagcaatatc tacttggatt cttttatcgg
     4441 ggctagtcaa gcgagcggct tctttaccca agccggtttc ttttaagaaa aatggatgat
     4501 cgattccgtc aacaagattt ttttgatcca aatcactagc aaaaacggac gctaattttt
     4561 taggtatttg aaaatcgaaa gcacttcctg cagcattttc ttttacccct ataggaatac
     4621 tatctgttcg caaaggagca taagcttcgc tattgagcca aagctcatgt tgatcgattg
     4681 actgagaagc atctcccgtc aagttgaagt aaacatgatt tgtcggattg aataaagtat
     4741 cttggtcact ggtgccgcga gaggtcactc gccagatatt atcttttgtc aaagtatagc
     4801 ggatttccac atccattgta cctggaaaac catgttctcc gtcgggactt gtagtagtaa
     4861 aaatgacaga agcttcattt tcgccattca aaatgacata agaccatttt tttaattcaa
     4921 atccaggagc accaccatgc agattatgtc cagtttttgg atctgtggct aattggtaag
     4981 tttttccatt gagagaaaag tggccatttt cgattctccc tgcggtgcga ccgatagaag
     5041 caccgatata cggatctttt tcgatatatt cttcagcggt gtcaaatccc aaaactaatt
     5101 ctcgatcatt tgattttaaa ctgacgattc tagcacctaa atcagagata gaaagtgcta
     5161 gcttgttttt gttctccaag gttatcaagt gataaccttg gccaaaatct ttttctgtaa
     5221 tcttcatttt caagatcctt tcttaatggg aactactgca tcgtgaagtt catcttccat
     5281 tgtatcagct ggagccaatt tgttttccaa tttctcaacg atttgtgcat gttttttctc
     5341 tgtcaatgta atcttccaag caaaaataat agcggagaaa atcatcaaag cagcaggagc
     5401 atagaatgca taagctttga aagtcgttac gccatgagca gtgatatcag cagcagtagc
     5461 attaccagtc atcccagaag cgatagcgac aaaccctacg atcccgttag acaaagcacc
     5521 agcgatttta tctagtaaag ggcgaattga tagagtaaca gcttcgttac ggataccatt
     5581 tttccattga ccatattcaa cagagtctgt gatcgtcatc aaagcggata agaaaatcaa
     5641 ttgttgaggg aagtagaaca atactagccc gatgataacg attggcaaac ttgaacctgc
     5701 gatcgtaaag aaaaagtaag aagaaatcat cattgcgatc cctccaaggt aaacgtagcg
     5761 acgagagatg acgcgtgtaa gcattgggaa tagtggaaca gctactaaac caagtaaggc
     5821 agcaactaga ccaacgatcc aaaattcagt tggttggcca agtacatatt tgaagaagta
     5881 gaataatacg gcagttgttg ctgcattggc aatcgcataa gcgatataag caagggcaag
     5941 ccacatcaac tgatcatttt gagcaactgc tttgaacaca tctttgatcc cagctttttc
     6001 attttgctga cggatcgcag tatcgacttc ttttgtgccc aaggcagtga taatcgcagt
     6061 accgcctgaa ataacagcag caatcacacc gaaccagaac cagcctgaag cgccttcaat
     6121 atgtttatga gtaacaagat aggtaaagaa agtaacgata gggacaacga ctagagtagt
     6181 accatttgca cctagagaag agccgaaacg agcaaacgta gcaaattttt cacgttcctt
     6241 tgtttccgta ctcaaagctg gaatcattga ccaaaaagcg atatccttaa atgaataaaa
     6301 actatccaat aagatgaaaa cgattgcaaa taatatgata aataaagtcg tattatgagt
     6361 agctaagcca aagaaattgg taaagattgc taccaagagt acggaactaa caacgccgcc
     6421 gataactaac catggtttga atttaccgta ttttgtacga gtattatcga tgatgccacc
     6481 aatcaaagga tcaaaaataa tttcaactaa gcggatacct acaactaagc ttgtaactgt
     6541 agcaatcatt tttgcattcg ttgccttatc tgttcctgcg aacatctggc ttgtcacgaa
     6601 taccataaaa taagtgctta atgtaacata gaatgtgtcg tgtccaaaag cgcctaaagc
     6661 gtaggacaca cgttggatca ttttgctttt cataatctgt attcctctcc tgttcaatat
     6721 ataatactaa gtaaaccggt tacaacgaat aacaaagatc ttttaatgac ttaaacttaa
     6781 caagaaatcg cttccttttt aatatatttc tgtgactttt atttaacttt ttgttgcatt
     6841 atactaaagg agtaaggagg gatagacgtg gagcaatcga tttttttaaa aaaggaacat
     6901 caacaagtag aaaaagaatt gtattttgat ttttgtggtt tctctaaaac gatgccgttt
     6961 cattcttttg gtcctgctat tcgcaatgat tacatcctgc atgtcgtgat ggaaggcaaa
     7021 ggatcttatc atgtgaaaaa tcaacaattc caattgaaaa aaggtgatct gttcttgatt
     7081 cgtccaggag actcgacgtt ttacttatca gatggagaag atccgtggat gtattgctgg
     7141 ctttcatttg gtgggagtgc tgcagaagaa atcattcggc agtcgctttt cagagaagac
     7201 caatacacga tggtctcttc tgacatacaa aaatatatcg acattatttt agaatgcatg
     7261 aaagatacgg aagaaacctt ggaaaatgaa ttacagttaa cagcattaac ctaccgtttt
     7321 ttatcagtcc ttttaaaaga tggcggaaag gtcagcggtg gtgtacaaaa ggttcattcc
     7381 tctttagctg ttgaaacagt caaatacatc gaggcacatt atgctgaaaa attgtctgtt
     7441 gaggaaatcg cccagcattt agcggtcaat cggagccatt tgtcgcgagt attcaaaaat
     7501 catatgggta ccagtatcaa ggaatatttg atcggggtac ggatcaatcg aggggcgttt
     7561 cttctttctc tgacggatga atccgtggaa agtattgcct atcaagtagg gttcaatagt
     7621 ctagtcgtat ttagccgaat gttcaaaaag agtaccggag aaacggctac gaactatcgt
     7681 aaacgtatga aaaacgaaaa ttttaagaat attgctttgg ataccttgaa aaaacagttg
     7741 gaaaaacaaa ccgcagtttc ttggacaacc tgattagtct ggttaaaatg acaagcatcg
     7801 gcaaaagaaa cttgtggcgg ttatgctaca ctgtaaagcg aagactaaaa aggaggaaaa
     7861 gggatgcgta cacttatcat cattagccat ccaacgatcg aagaatcttt aaatcagcaa
     7921 tttttcaaag aagcttctgc tggtttacca gctacttggc atcatttgga atcaagttat
     7981 ccagacggaa aaatagatat agagactgaa ctacagctct tggcccagca tgatcggatc
     8041 attttccagt ttccgtttta ctggtatagt tctcctgctc atttaaaaat ttggcaagat
     8101 aatgtcttgg aacgagcagc acatgtatta aaagggaaag aactaggaat cgtattgaca
     8161 acaggtgtgg cggaaaaaga ataccaagct ggaggaaaag aagaatatac gatttccgaa
     8221 tttttgaggc cgtatcagcg aatcgcaaat aaattccata tgacctatct tccgccattc
     8281 gttttagcac aatttatgta tttatcccag gaaaaacgtt gggagaagct aattgcttat
     8341 cagcagtatc tctcattgga aggcaaacca tcgctgactc agcggattga ctggtttatt
     8401 caaagagtac aggaaaatca gaaaatgcag gaagaagaat ctgaaaaaca agcatatatc
     8461 gtagaagcac tgactgaggc aaaagagcag atcgaagact tgacctttac actgcaagaa
     8521 atgaaaggaa cttcattatg acaaatgatt ggctgatcca acaattagat gaattaagcg
     8581 aaaaatcaga atatcaagac cgtgcgctct tatatgaaat aaagaaactg atggctgaga
     8641 tagacgaacg aacaatgcag ctgcaaggag aaatcgacgg acaatcttgg aatcatctca
     8701 actggtgaaa ttttcttata aataacaaag ataaaaatgt gcacagcgac aattatttgc
     8761 tacaataaga atatgaagcg tttgataacg ctattataca taaaaattgg tggtgaaaaa
     8821 aatgtatgat gaaaatgagt ggggagaaaa tgttcggatg acccgaagaa gacagcaaaa
     8881 acaggcagct cgtaagcgga tgatcatgat tggggcagca gttgccgtgt ttttgatcat
     8941 agcaggagga gttgcgggtc tatattttca tggaaaaaat tcgtcagaag cgaaaagtac
     9001 gaccataacg gaaacaacga caaagaaaac agagacgaaa caaacaagtg agtcatcaac
     9061 taaaacaaaa gagacacaaa cttctgaaga aaaaacagca aatggtttgc cagagcaact
     9121 tcaagcaatc gtaaatgatt attcgaaaaa gatcgaagaa aagacgcctg cattgattga
     9181 agaataccag acagaaattc agggaaatca agagggaata gctggtttat cagcagttgc
     9241 taaccaaaaa gcacgtgagc ttcaagcaat ctccgacgaa gggatcagaa aattacgtgc
     9301 agcgtatcag gcagcagaaa ataaagacgg tgtagactta gatactttga tcaatcaatt
     9361 atcggctaat tatacgaatc atgtcgctaa gatttccgat atttatttgc agactagcgc
     9421 ctctcttcaa gcagagtcaa caagcacaca agacacaaca tcttctgatt ctgagacagc
     9481 agagtcggta gaagcttctg aaatgcaagc tcgaacaaca caagacacaa cagattcctc
     9541 tgaaactaat accaacagcg cgacttcaca ggctgcgaca accgtagttt tggaaggaga
     9601 aggtccaaat caaatagccg aacgaactgg cgtaccagta gaaacgatct tgtcgctgaa
     9661 tgggatgagc atggagaatt atttcctgac tcccggtgag acgctaaaat tgaattgata
     9721 aaagcaataa aaaagaacga agcttccact aaggtgaagc ttcgttcttt ttttattttc
     9781 cggttctttt ttcgcgtaca aagtaatgga tcgtcatgta aagcaagaaa acgagtccaa
     9841 taatcaatga gattctcgtt tcaggattga taaacatgaa aatcaatacg atagctaaaa
     9901 aagcaagtgt cacataattc gtataaggag atagtggcat tttaaaagga tgatcatgca
     9961 ttttatctgc gtgcttcttt ttgaagcgaa tctcgctgat caaaatcact acccaaggaa
    10021 ccataccagg caaaatactt gagctgtata cgaaaacgaa aatattttct gactggttga
    10081 tgaacattgg tagaagataa tttaagatca gaccaattaa gattccacct gagattgcta
    10141 cgacaggata aaaaggcgca ccgtgacgag atagtttcag aaatttttta gacatctttc
    10201 catttatggc aagtgtatag accatacgac ttgcgctgaa aatccctgaa ttgcaacctg
    10261 ataatgccgc tgtaatcacg acaaaattaa tcagtcctgc agcaaatgta atgccgactt
    10321 tagaaaaagt ttgtacaaaa ggtgaaccaa tctcactcaa ttgattccac ggatagatac
    10381 tcacaatgat aaaaatagca ccaatataaa aaatcaagat tcgaccgata gtggactgga
    10441 cagcttcaat gatcgtcttt ttaggattct ctgcttcacc agcagtcatt ccgatcagtt
    10501 cgattccttg ataggaagca acaacgatcg ataatgcaaa gaaaaatcct ttgacgcctc
    10561 cagtgaagaa tccaccattt ttccaaagat ttgaaatccc tactgcctga ccatgattac
    10621 caaatccaaa taagattacc ccaagacctg caatgatcat caaaatgatc gttatgactt
    10681 tgatgatcga aaaccaaaat tctaattcgc caaacgcctt gacagaaata agatttgcca
    10741 gcataagaaa caagacgaca atgattccgg gaataattgc cggcaaatca ggccaccaat
    10801 aattcatata agatcctaca gcaatcactt cacttattcc tacgacaata tattgaaaaa
    10861 tattactcca agcggtcaag tagccagcca caggatggat gtactcgctc gcgtaatttg
    10921 caaaagaacc agtcgaagga tcaacgtaaa gcatttcgcc taaagcacgc ataatcaaat
    10981 acaaaaagaa tcctgcaatc gcataggctc ccatcactga gggccctgtc caacgtatgg
    11041 ttgaggaggc acccataaac agaccgacac cgatcgttcc acctagtgcg atcatttgga
    11101 tatgacgggc actaagactt cgagttaact tttcttcttc catacaaaaa ctcctttcgt
    11161 tgttgagtca acaagaaaaa ataatcaaac gtttcaacac atgtttttca ttattaggtt
    11221 agccttattg aactcttgat gtgattctac agaaaatttt cataaaatgc gaggaaaatt
    11281 cgaaaaaaac tttttcatca ttctacttga aaatgctttc cattttagtt aaactatatt
    11341 taaataaaaa gttaggtaaa cctaaaaagg agtgtaagat ttggaacgta cggccattgt
    11401 aattcaagac ctttcagccg cttatcaagg gaaaactgtt ttgaaaacta tcaacttgac
    11461 gataaatcca caaaaaatta ccggtattat cggaccaaac ggtgcaggaa aatcaacatt
    11521 tatgaaatct ttgctagagt tgataccagc tactactggt aaagtcacct tttctggaca
    11581 gtcggtaaac tcagtccgta aaaaaatagc ctacgtggaa cagcgaagtg aattggatct
    11641 ttcctttcca attgatgtat taggcgttgt acttttagga acatatccat ctttacgaat
    11701 tggacaaaga cctgggaaaa aagaaaaaga acgtgcaaga caagctttga aaaaagtagg
    11761 gatggaagaa tatgccaaaa gacagatcag cgaactatcg ggtggacagc ttcagagagt
    11821 ttttattgca agagctctag cccaaggagc agaatggatc tttttagatg aaccattcgt
    11881 agggattgat gcgttaagtg aacgaaagat ctttgacatc ttgcaggaat tgaagaattc
    11941 aggaaaaacg attttgatcg tccatcatga tcttcataaa gtagaagaat atttcgatga
    12001 ggttattctt ttaaataaac agctgatcgc ttccggtcca gtacaagaag tctttacatc
    12061 gaagaacctt caattggcct atggtgagat catcagacat ttagtaaaag gaggagagaa
    12121 aaaatgatcc aatcctttat tgatggatta atgaattatc aattcctgca atacgctttg
    12181 gtcacttcca tgcttgtagg acttgcttct ggagtcatcg gttcttttat tattttacga
    12241 gggatgtctt tgatgggaga cgctatctct cacgcagttc ttccaggtgt agccatttct
    12301 tatatgtttg gttttagtta catctttgga gccactgctt ttgggatgct aacagcagca
    12361 gtgattggtt tcgtgactca gcggagccga ttgaaaaatg atacagcgat tggaattgtc
    12421 tttagctcct tttttgcatt agggattatc ttgatttcat atgcccagag tgcgacagat
    12481 ttgtatcata ttttatttgg gaatgtctta gctgttaggg aaagcgattt gttgctgaca
    12541 gcactcgtta gtggaatcgt cttgatattt gtctttttct tttataaaga attgaagatc
    12601 acatcattcg atccgacaat ggcaaaagct tatgggctga acacatcgct gatccattat
    12661 cttttgatgt tctttttgac attagtggct gtagtcagtt tacagacagt aggaacaatc
    12721 ttggtgattg ccatgttgat cacaccagcc gccacggctt acttgctaac gaaccattta
    12781 ctgaaaatga tcattacagc tgcaggaatc ggtatgctaa gtgcagttgt cggtgtgttt
    12841 ttcagttata gttacaattg gccatcagga gctacgatcg tgttagcatg taccgcattt
    12901 tttatccttg cttttttatt ttctccaaca aaaggaattt tatttcagaa aggaagcaga
    12961 tagatgaaaa caaaaaaatc actcttttta atcttggcag ttagtttctt ggtattagcc
    13021 ggttgcggaa agcaggcttc tgaccaagcg gatgaaggaa gtaaagaaaa gttgtcagtc
    13081 gtggctacca attcgatctt ggcggacatg gcaaaagagg tcggaacaga tcaaatagat
    13141 atccacagta tcgtaccagt cggaacagat ccgcatgaat atgaagtact accagaagac
    13201 atcaaaaagg caagcgatgc agatgttatt ttatacaacg gtttgaatct tgaaacaggt
    13261 aacagctggt tcgataactt gatggaaacg gctaaaaaag aagaaggaaa agattatttt
    13321 gcagttagca aaaatgtaga acctctttat ttaactagcg gtgaagaaca tacaaaagca
    13381 gatccccatg cttggctaga cctatctaac ggaataaaat atgtggagga aatcgcacgt
    13441 atattctctg aaaaagatgc agaaaatgcg acactctata aaaaaaatgc agaagcatat
    13501 gtggaaaaac taaaagaatt agatacacaa gcgaaggaaa gttttgcttc tatcgaagag
    13561 aacaaaaaat tattagtaac aagtgaaggg gcattcaagt atttttcacg agcatatgat
    13621 ttgccagcag cttatatatg ggagatcaat acagaaagtc aaggtacgcc tgatcaaatg
    13681 aaagcgatca ttgatcagat aagagcaaaa gaagtaccgg ttttattcgt ggaaaccagt
    13741 gtagactcaa gaagcatgga acgggtagca aaagaaacgg gtttgaaaat ctatgataaa
    13801 ctgttcactg attccatagc aaaagaagga gaacaaggag attcttacta tcagatgatg
    13861 aaatggaata ttgaaacgat ccatgaaggt ttgtcacaaa caaaagaaag ctgaaaagtg
    13921 caaggtctag cttgaggagc agaccttgca ttttttataa gagatatgaa aaaaaattat
    13981 gctgtttttc gtcgtacaga tacctttttt gaagaaattc cgttatagtt agagtaagga
    14041 aggaaggtgt ggctatgaat tttacacgta aagataaata tacgattaaa gaagaagtct
    14101 ttcgattgat cagccagcag gaaaataccg acaaggatca agaccttcaa tttcttgaag
    14161 cagaattagc taaattattc atagcaaatc aaaatgagac aacagaacca gatgaagagc
    14221 tgtacatagg caaatggttg tttgatgaat ttcagccttt agatgaaaat accggtatcg
    14281 gttattttgt gttcctttct ccagtcggct tcacacctgg cgtattagtt aaacaaaacg
    14341 acgaaataac cgtcaaagca tggcctgcat tattttcagt catcaaagca gaaggagtaa
    14401 tcaaaaagat ccagtcggcc tattataaaa gtcaatttat tgccaacagc aaattaatga
    14461 atatgtttta taatcgtcta gtagaaattg ctaggcctgg gaaaatcccg caaacattca
    14521 cgtacacaca gcaaaaaaat ctctatgaag aagtattgta ttcccgcgat ctgtctgagt
    14581 tttcgccatg ggaagaggta aggctccatg aactgttaag tacaaaacca gtttcattga
    14641 atttttttaa tgcattagat aacaaaagaa aattaatttc aacaaattat tgggaagtat
    14701 ttgctgcaag aaaaaaatgg atggaagaaa agaagattgc cgatcagcag atccagtttg
    14761 tccagctgaa aaaagaaaaa ctatattatg ggttatacag ccgttaacag caagcgagga
    14821 agaccatgac attaaaagaa gaaatcatac aagaaagcaa acgattagga attgacaaaa
    14881 tcgggttcac cacggcggaa ccttttgatt cattaaaaga atcgttggaa gaacaaaaag
    14941 cagctggcca tacttctggc tttgaacatc caaatatcga tgagcgtctt tatccagaaa
    15001 tgacatttga tcagccgaaa tcgatcattt ccattgcact tgcgtatcct actaggatcc
    15061 atgaagaagt gcctagggat gaaaaacgcg ggcagtttgc tcgtgcttct tgggggatcg
    15121 attaccatga tatactcaga gaccgtttga accgattgat cgaattcatc caaacaagag
    15181 cagaaaaatg gcaaaaagaa gaggaatggc gtctagctcc gcaagtagat actggagaac
    15241 tgatcgatgt cgctgtagca cagcgagctg gtttaggatt tattggacga aacggattgt
    15301 tgatcactga agagttcggt tcatttgtct atcttggtga gatcgttact aatattgtat
    15361 ttgagcctga taaaccagga gaatttggtt gcggtgactg tatgcgctgc gtgactgctt
    15421 gtccgacaaa agcattgctt ggtgacggaa gaatgaatgc ccagcgatgt ttgtcttatc
    15481 aaactcagac aaaagggatg atgccggaag aatatcgtaa aaaaatgcga aatgtcattt
    15541 atggatgtga tatctgccag ttagtctgcc cttacaatca aggaaaagat tttcattttc
    15601 atgaagagat ggaaccaaaa gtagacgaaa tatatccaaa attaaagcca atgctttcga
    15661 tgtctaataa agacttcaaa cagcaatttg ggcatttagc tggttcatgg cgtgggaaga
    15721 aacctttgca gagaaacgca ttgattgctt tagctaatct aggtgatcga acggcattgc
    15781 ctgagatttc agaatgttta acagatgtcc gccctgtcat cagaggaaca gcagcttggg
    15841 ctattggacg tctaggaaca aaagaaccgg aaaaatggct tgatgtatta gatgaaatat
    15901 tagctaaaga aacagaagaa gtcgttataa acgaattgga acatgcccag aaattattgc
    15961 ggagaaagtt aaaataaaaa aatagttaac attattcaaa gtttttgtcc agtaattgtt
    16021 gtaacaatta ctggtttttt tgtatttttt acattttttt aagagaattt gataaaaatc
    16081 aaaaattttt ttgctttttt ttatggatta ttgcagtgaa tgttacagaa tctgcgtttt
    16141 tcattcaaaa ataaaaaaac atagaaaaac gattactttg cttatacaac aaggctttta
    16201 tggttttgta tcaaaaaaat atttgtttaa atgttacaaa t
//
LOCUS       DACEKH010000064        15508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEKH010000064 DACEKH010000000
VERSION     DACEKH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.067"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(125..1234)
                     /gene="rpoD"
                     /locus_tag="IW662_001884"
     CDS             complement(125..1234)
                     /gene="rpoD"
                     /locus_tag="IW662_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0866705.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1258..3099)
                     /locus_tag="IW662_001885"
     CDS             complement(1258..3099)
                     /locus_tag="IW662_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0866706.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3281..5674)
                     /locus_tag="IW662_001886"
     CDS             complement(3281..5674)
                     /locus_tag="IW662_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0866707.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5781..6641)
                     /locus_tag="IW662_001887"
     CDS             complement(5781..6641)
                     /locus_tag="IW662_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ0866708.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6700..7221)
                     /locus_tag="IW662_001888"
     CDS             complement(6700..7221)
                     /locus_tag="IW662_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ0866709.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7541..8260
                     /locus_tag="IW662_001889"
     CDS             7541..8260
                     /locus_tag="IW662_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0866710.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            8286..10877
                     /gene="mprF"
                     /locus_tag="IW662_001890"
     CDS             8286..10877
                     /gene="mprF"
                     /locus_tag="IW662_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0866711.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11065..12252)
                     /gene="tuf"
                     /locus_tag="IW662_001891"
     CDS             complement(11065..12252)
                     /gene="tuf"
                     /locus_tag="IW662_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0866712.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12620..12976
                     /locus_tag="IW662_001892"
     CDS             12620..12976
                     /locus_tag="IW662_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0866713.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(13217..14221)
                     /locus_tag="IW662_001893"
     CDS             complement(13217..14221)
                     /locus_tag="IW662_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ0866714.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            14387..15238
                     /locus_tag="IW662_001894"
     CDS             14387..15238
                     /locus_tag="IW662_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0866715.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            15255..15500
                     /locus_tag="IW662_001895"
     CDS             15255..15500
                     /locus_tag="IW662_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866716.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         4509 a         3219 c         2743 g         5037 t
ORIGIN      
        1 aaaataatga atgaatttcc ttcctataga aaaaaaagct tgagactgcg tagtctcaag
       61 ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg tcaatagtcc
      121 acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc aattttctta
      181 atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaatact ttccctactt
      241 cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg acattctctt
      301 cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac tcataagctg
      361 cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag tgagagtcat
      421 cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc aagatttctc
      481 ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta ggttctcttc
      541 caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt tcaaccatat
      601 gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa
      661 tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg
      721 ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg cctcgtccta
      781 cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga cgctgtttcg
      841 cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc gcagttaaaa
      901 gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt tttacaccgg
      961 ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca ctcttcaagc
     1021 tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct tcaatctttt
     1081 ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc tgattggata
     1141 aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct gctactgctg
     1201 cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct tgagtagcta
     1261 tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt caagctcaag
     1321 ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg ttatttcatc
     1381 agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg gcgaactttc
     1441 ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt catcttgcaa
     1501 aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg catcgaataa
     1561 aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt ttaaaatatt
     1621 acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct tttctacttg
     1681 cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt gttgactttc
     1741 ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat
     1801 cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat cttgttcgat
     1861 tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga tcttgtcttt
     1921 ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa agactgtttc
     1981 gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga gatattcatc
     2041 aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt tttgccgaag
     2101 caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac
     2161 caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac ttgtccccat
     2221 agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca taaacccttc
     2281 gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa gattaaataa
     2341 aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg gctgatcttt
     2401 ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga
     2461 attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc catcatcatg
     2521 cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat tcttgaatac
     2581 ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt caattaattc
     2641 taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt ttgtatgcaa
     2701 tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc
     2761 agattcagga ttttggacag ctatagtacg ctgacgccat tgatcttcta acggtatctg
     2821 ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta gttcttgaag
     2881 aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc
     2941 tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca agtaattttt
     3001 accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg tttgactgcg
     3061 tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc ctgctctatt
     3121 gatatctttt gggtttaaca gaagatattt caggagaata accggtaaaa aatcgcacta
     3181 actgaaatca gtgcgatttt aaaggacaca attatacatg tttgattgta tcatatttca
     3241 ttgctttttc aagtataaca catttctatc tcattaagca tcaatttgct actcttttca
     3301 gtaactgatt ttgaatatca gccttccagt acaatactcg aagaacaata tttacgagca
     3361 tgaatacaaa gaagtatcct acgctctctc ccaaccctaa gtttacttcc aaaattccat
     3421 ggaacagcat tgcagctaca tatatcccta cgatcgtcgt tactgctagg aagatgcgca
     3481 atgggttgaa gggataacaa gcttttacaa ctgccatgca actgatccca atcagcaagt
     3541 aatacatcaa agtagtagtt tcagcatttc cccatgcgaa tgtttttcca agtagataca
     3601 ccgcaatgat attcactact acaagcaagg catttggtaa agcattaata agtgctgtag
     3661 gcaaataacg gtaagtgatt tttcttttat cttgttcaaa agataaaaag aaggaaggat
     3721 acccttcaat cgctaaatca atcagcgtga tttggatagg gataaacgga aatccaattg
     3781 ctgtaacggc acatataatc gataaaataa atgaataaat cgttttgata aaaaagatgc
     3841 ctgagacttt cgtcacattg ttgaccactc ggcgtccttc aaacaggact tctggcaatg
     3901 tagtaaaatc agagtccaac aataccagat tcgcaatctg acgtgttgct ccatcacctt
     3961 ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa aacatcattc acaccgtcgc
     4021 ctgtcattgc aaccgttctg ccgctttctt tcaattcatt taccagcaat ttcttttgct
     4081 gcggactcac tcgtccaaaa accgtatagc gatgagcagc ttctcgcact tccatttctt
     4141 cagtaatttg agaaagatcg acatacgcat cataattcgg taatcctgca cgtctagcga
     4201 tattggagac agtaaaagga ttgtctccag aaatcacttt cagatccaca ccttcctcat
     4261 gaagataagc tagtgtttct tgagcgtttt ttcggattgg atcatcgatt tcaaacagag
     4321 caaccggctc aacaaatgct ggtttttctt cgttcaattg ctgatttgga gcgatcccca
     4381 acataagtac gcgatagcca ttcgcttggg ctgtttttag actgtccgga agccgatctt
     4441 cttccaccaa acgttcaggc gctcctacgt acacgactcc cagttctgga aattccatcg
     4501 cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac cgattagaaa
     4561 gctcaaaata atctctcata gcccgcatag tgatgttgtt atcactgctt tctgccagat
     4621 acgtccccat aatttctggg atggctttct cgtacagcgg atgtagtgct tctacccgct
     4681 ggatactcat tttcccttca gtaatggtcc ctgttttatc caagcataat gtatcgacat
     4741 gggccagcgt ttcaattgaa tacatgtctt gaacaaggat tcgttttttt gccagtttgg
     4801 ttacccctgt cgttaatgca atacttatta gcaataccaa accctttgga agcatgccta
     4861 acaaagctgc tgcagatgca ataacagaaa ttttgataga agcatcgcgg atgaacagtg
     4921 cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa actagtgaat ttggatactt
     4981 tgcgaatcga atctacaagt tcagaacgaa tagctttgtg agtctttgcc tcgctagtca
     5041 agcggacagc ataattatct gcacctactc ggataacttc agccatgaca gaaccgcttg
     5101 tcaaaaagct tccagaaagc aattcagctc cagtttcttt gacaatcaaa tcagattcgc
     5161 ctgtcaaaag agcttcattt gcttctgccc gtccagatat aacctgtaaa tcggctggta
     5221 tctgttctcc tgctccgatt tttaccaaat ctcctaatac cagctcttta gtagaaatcg
     5281 attgttcttt accgtcccgg atcactagta cttctgattg ggaaacaatc gataatttcc
     5341 gtacaagatt tcttgcatgt atttcttgat aaattccaat aacgatattg acgacaataa
     5401 ttgctaaata cgccatgttg gaatacgcac ctacaaataa cagacaaatc ccaataccaa
     5461 agttcagaaa attgaataac gtcaatatat tatcgctgaa tatttgtttt gtactttttg
     5521 atgcatcttc ctggtagtca ttttgctggc cagaagccat tttctgaact acctcttgct
     5581 gtgtaagtcc attcattttc tttttctcca ttttccgcca actttcaaaa ataatcacgt
     5641 gtttaagtct agcgtgttcc aagattaaaa acaacttgtt tttataaaat ttatagaaaa
     5701 acacacgaat gattttacaa aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg
     5761 tttttattat caatctatct ttacttatat tgttcctcca aacggctcat ccaccaacct
     5821 agtgctgtac cggctataaa aagaacagga caagctagta ccccagcaac tcccaatccc
     5881 gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg
     5941 aatagcttgc tatatgcctt tgagaaaatg taatccatga tttttgacaa tccaagaaca
     6001 caaagaatcc caccaattcc gatcggcaca atcacttcaa gtcgtacttc tttaaatccg
     6061 tcagccatcg ctttatacaa tcccatatac accaaaaaat tagaagggct caatcctggg
     6121 acgatcatac ccaaaccaat caatccgccg gcaatcagcc atgttccaaa attctgatcg
     6181 acttgcccgt caaataattg agcgccgtat aataaaaaca aataagccag aacaaaagta
     6241 acaaccatga tgatcacatc agaagtatct cttccttttt tccctgcttc tttccataac
     6301 gcaggaacag ttccagcgat acatccgaca aaaaaccata atatcgttgt ttcatacgca
     6361 cctaataaaa aactgactaa aaaagaaagt agaaagatgc ctgtcactcc gcctagacca
     6421 acaggcagaa aaaacaaaac attttctttg aaattttttg taaggtgggc taaaaaagaa
     6481 atcagccgct catacatgcc aaagacagct gctaatgcac caccgctgac tcctggcaag
     6541 ataaatcctg aaccgataaa cattcccttc acaaaccgta acatccagtc tttggttgtg
     6601 taccccactt cttgattttc cattttttgc cttttttcca ctatttgcgc actccttctt
     6661 tcatttaatt ttattcatga ttgaacattt cctgctattt tacttaaaga ctttttcata
     6721 ggtctctatt attttttcat tcattttgac aatatcagga taaaacatgt tacctccatt
     6781 atgatagaga tagccgccat tgtaaagcaa ggactggatc cgccaatatc ggtattgttt
     6841 cccttcctca tttcctaaaa gtgggctcag cacatcttta gaatacgctt ctgctaattc
     6901 tactgtgttt tctccgccat gattttttac ataggcaata taatctttcc caaaattata
     6961 tgcttggaca gctgtccata aatcgcatcc ttgtttcttt gcttctacta tcatttccgc
     7021 caaatatttg acaccttgat caatactgtc agctggatta tcaatgatct cagcttctcc
     7081 atgcagactt tccgagctct gcatgagatc tgtccctttt ccttttgatt cggtcaatat
     7141 tatcgccttg atcaaattct cgtagttttc catatgataa ttttttgcag ctgttgttat
     7201 ttctatttga tagctttcca ctcgttttac cgaacgataa gttcttcctc ataaaaacaa
     7261 caccgcacca ataaccgtca gaatgatcat gtaaaaaaga atacggataa tagataatcg
     7321 ttttcgcttc atttcatcac ctagttattt tgacatcttt ttttatctta ctatttcttt
     7381 atcttcttgg ctatttcttc cttgatttct gtgtacgcct taagaaaaaa tgaattttcc
     7441 tgtatttttt gacatcttac gaaaatgatc gttgtttttc agtaaaaaca attgcccaat
     7501 gactgatata atcgttatac tttacacagt aggtgattat gtgaaaaaag aaaagaaata
     7561 tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag agcggtcaag gcttccctct
     7621 ctttttactg catggcaatg acggcagcgg ccgctttttt tcagaacagg tccctgtact
     7681 cgaacgctac tataccgtgt atctagtaga tagccgagga catggtcgtt cgacaaacga
     7741 agcatctatg ttgaattttc agttaatggc cgaggatctg aatacgatca tgttgctgga
     7801 aaaaatcgat caggcagatt ttcttggatt tagtgatgga gcaaatctag cactggtatt
     7861 tgccagcagc ttccctaaaa aagttcatcg attgatttta aattctggaa acacgcttgt
     7921 taaaggcgtt cgtttttctg ccagagtcat cagtaatatt cattatgcat gggtatggtt
     7981 actctcttta tttcgcccaa gtcttcgcaa aaatctttta gttatcaaac ttttgctgca
     8041 tgacatcggg cttacagaaa atgatttaaa aaaaatcaac tcccccactt tgatcatcgt
     8101 gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat atagctaaaa ctataccaaa
     8161 agcttccttt gtgcttgtga aagaacaagg acatgaatta gctagaaaag atcctgaacg
     8221 cttcaatcga gaagttcttc aatttctttc tgaaacataa ctattaggaa tagaaggtga
     8281 tttatttgtt aaaaaatacc atacaatggc tgaaaaacca cctaggactg ttcaaaacaa
     8341 tctttttgat ttctgtcatt gtcattattg tcggtgaatt gatgtccatc ggtaaaactc
     8401 tatctatcca gcaattagga gaaacgtttg ccactatacc tgtatggaaa agcggactaa
     8461 tgctattgat tggtcttatc tctgtgttac ctatggttgg ttatgatatt attttgaatc
     8521 ggatgcttga acaaaagcag aatcctcgtt acctgtttga gacaagctgg ctgatcaata
     8581 cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat
     8641 atggacaaaa gaaagagagc agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt
     8701 tgatggctgg actttctatt tacagtctgc tttctttttt acttgtagtc ttcacgccag
     8761 ttgctccttt tttgaagcaa tattggatat ggttgatcgg cggcggtcta tattttcctg
     8821 tcgtcttttt atttactact ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa
     8881 ctcaaggaca gctattgcta gtttcattgt tggaatggac cggtgtcctg tttagtttcc
     8941 tttctgttgg ttatttaatg gaaatccgga ttgatctgtg gcaaacaatt ccattattca
     9001 ttgctgcttc agtgatagga atcgtgtcta tgattccagg agaaattggg agtttcgatg
     9061 taatgatgat tatcgggttg tccgctattg gtgtgccaag agaaatagtc gttgtgtgga
     9121 tcttgctcta tcgcctgttc tactacatca ttccgttttt gattggaatc gtctttttct
     9181 tcaaaaatat cggttccact tttgatcagc gttattctgg tatccctaaa caattagcaa
     9241 cagaaattgc gcataaaatc gtggttgtct tattatactt ttctggaatc atgctagtac
     9301 tttctgccac tatcccgcaa gcttttacag agtttcgctg gcttcatagt ttgaacccat
     9361 tgaaatttca ttttattatc caatttccta gtatactatt gggatttctt ttaatcatta
     9421 tgggcagagg gatcgcagca cgtgtgaaac gagcatattt gcctacaatc ttcttgattg
     9481 ttctagcttt actttatgtt ttattaagtg attttagttt cacaccggtg gttttcttgt
     9541 ctattctgct tctgatcatt cttgcttcta aaaacgaatt atttcgggaa caattgatct
     9601 attcatggga gtggcgtacg atcgatggaa tcattattgg tgcactgtcc ttgctttaca
     9661 tcgttattgg ggtatacaat ctgccggatt tccctcatcg gcgtcaccat tttatttcct
     9721 ttttcttatt tccttctgaa aagatttggt tttcgggatt gttggccatc atcgctgtta
     9781 gtttcatgat cgttctattt gttcatttcc ttcaaggaga aaagaaacag attggcgaag
     9841 cctttaatga agaaaaggcc ctgaagatcc ttacaactta tggaggaaac tccgatagtc
     9901 aattgatctt cttaaaagat aagcggatgt ttgcctatga aaaagacggt gaaccaactg
     9961 ttcttctcca attcgcttgc ttcaataata aatgtattgt aatgggtgat ccttctggta
    10021 agaaagaaga ttttccagaa gcaatcgaag cttttatcga agaaacagat cgactatgct
    10081 atttaccagt tttctacgaa acaagcgaag agattgttat gattttgcat gaattcggct
    10141 acgatttcat taagatgggg gaagaagctt atgtagacct gaatagtttt acaacttcag
    10201 ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat cgaacgagaa ggatttacat
    10261 ttgatgtctt acaaccacca ttttctactg agcaaatgag tactttcaaa aatatctctg
    10321 ataattggtt gggttcacga aaggaaaaag ggttttcttt aggctttttc tctgaagatt
    10381 atctacaaag agcgcctatt gcagttgtca aaaataccca agaagaaatt gtcgcttttg
    10441 caacgatcat gcctacttac acgaacaata aagtaggaac gattgatctg atgcgatatg
    10501 attctaaaaa agcaccttct ggcagtatgg atttcttgtt cttgcatttg tttgcctata
    10561 tgaaagaaga acatatccaa tggttcaatt tagggatggc ccctctttca aatgtcggaa
    10621 cttccagaaa gagtttttta caggaaagga ttgcatactt ggtttacgag tttggttctc
    10681 atttttattc tttccatgga ttaagagaat ataaaagcaa gtacgctaca aactgggtct
    10741 cacgttatac tctatattct agagatagtt ggattgcata tgttatgatt gctttgctga
    10801 ttattgataa tgcgccagtt gagcagacac aaggtagaat aagtggtttg aaaaaatgga
    10861 tacgaagaaa gtattaaaaa ctgcataccg atagatgaac actctcatga tgaataaaag
    10921 agactgaagt aaaacgaaag ttcatgcttc tacaatctct tcttatacaa ttgaagaaca
    10981 aagagcggtc caaacagaaa tcctgtttgg accgctcttt tattttaaac atttttcatt
    11041 accaaaatat tcgagtcgcg tttcttatgc ttcgatctct gtaacgatac ctgaacctac
    11101 tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt tcaacagcga ttggatggat
    11161 caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc atttctgtat cttctggcaa
    11221 tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac tgtggacgat agttgttgaa
    11281 gaatggagta tgacgtccac cttcttcttt tgtcaacacg tacacttctg ctttgaattt
    11341 tgtatgtgga gtaatggaac ctggtttagc aagtacttgt ccacgttcga tatcgtcacg
    11401 ttggatcccc cgtaacaaca cgcctacgtt atccccagct tccccgtaat ctaacgtttt
    11461 acggaacatt tctacacctg ttacaactgc tttttgtgtt tcaggtttga tccctacgat
    11521 ctctacctca tcaccgacac gaacagcacc acgatcaata cgacctgagg caacagttcc
    11581 tcgacctgta attgagaaga cgtcttccac tggcaatagt aatggtttat ctgtatcacg
    11641 ttctggtgtt gggatatatt catctactgt atccatcaat tccataatag cagcttcagc
    11701 atctggatcg ccttgcaatg cttttaatgc ggaacctttg atcacaggag tgtcatcgcc
    11761 tgggaaacca tattcgctca ataactcgcg aacttccatt tctaccaaat cgatcaattc
    11821 ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc aggtatttta caccaacttg
    11881 gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac
    11941 taagattgca ccatccattt gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg
    12001 tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt
    12061 attgatcgta atcccacgtt cacgttcttc tggagctgca tcaatactag catagtcttg
    12121 aggatttgct aggccttttt tgcctaatac tgtagtgatc gcagcagtta aagtagtttt
    12181 cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg
    12241 ttcttttgcc ataatttaca aacctcctaa ataatataga tcactgaaaa atttcagctc
    12301 tcaccttttt attatagtct ttttttgact aaatcaaaaa gaatacgtcc gacttacttt
    12361 ttgtaagatt cgaactaagc ttattatacg tcaatattgg tcaaagtcaa ctaagagcac
    12421 tcgtctgtct tatacgcgaa aaaagtacct tttataaata aaaaaatgtg ttttttgaga
    12481 aaaacttcaa gatagtcctt aactttcttt tgaagcaata agaacaaaat gaggaaaagt
    12541 ttaaaattca gctgttgcca acaactttca atagacgaaa tcttctattt cgttaataat
    12601 aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta cttgtcgttt taggcatatt
    12661 tctcggcgga agttttgcag cttatcatta tttttacggc ggcgaagctt attatacaaa
    12721 aatcacgacg aatggcgaaa tgtcagctac ccaagcagat aacggagaga aattcacgac
    12781 ttatacctac aagcaagcgg cttataataa agatggtgaa aaaaaacagg tgacgcttcg
    12841 cgaagaaaga gaacatccat taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg
    12901 taaaggtgta ttaagctggg aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga
    12961 aaaaatcgat aattagtaaa tggtcttaaa attttattag ataaaaagag gctgggacag
    13021 aagcgtttaa ctccaagaaa taagaaggag ttgcttctgc ttccgccgtt tatacgtgtt
    13081 tagagcgtga gacaaaagtg ttctttactt ttgtctcacg ctcttttttt gttatattcg
    13141 tcttttccga tccttctctt gacacaattc atttaactga tcactggtac ttgaacacct
    13201 aatattctag tcatttttat aaacgattca atacttcatc cacatttttg atcatcacgg
    13261 atatcgtacc atccggatta ttcacaaatt caatcaaatc tgggttgcga tatacatcta
    13321 ctggtacgat cagttcgatc ccgtttgata gcttgaattt ttgttttcca aattttttct
    13381 cagaaatttc ttgtacttct tttacaggag gtgcttctgg gacaaatcct tgctcttgta
    13441 cttcttcttt aaaagccatt tttgccgtga tgttttcctt gaagactttt tccgcaatca
    13501 tctcatgatc caaccgacct ttttcttcta tcgtatcata gacagcttct ttcaaatcag
    13561 ccatgatatc aaattcttct gtctcaaatt tttttccaag atgcttggct actttttgga
    13621 tcacttttac attttcttct aaagaaggtg caggaacact ttcgatcact cttccagaaa
    13681 aataaagctc ttttttgcca gaaaactcat attttttttc aattaattcg taggtccaat
    13741 cagtcaaatt cacagcgaat gcttcatctg ctttttgtgt tttcgcccct agaattgctc
    13801 gattcaaaat caagcggttg tcgatccctg cttcgtctgc ttcaacaaag tgagtgtaag
    13861 cctcacgata attgactttc aataaagata agtacatcac tgtatctaat tcatacaaga
    13921 caacaaatac atctgcacta ggtgcttctt cactttcttg gtagatatcg aaccaccgct
    13981 gaacgattgc ttcacttgct tccacaaaat tttcttgagc gattttaagt gattcaacga
    14041 tcatgctttc ttctgctagt tgtccagttt ttgtttgagc agaagagatt ttttggatct
    14101 tcttttgaag aaaatcacga atatattctt ttgtcagatc cagctctttt tgtgaaaaga
    14161 ctggagaacc agtttctctg tcaataatat gcagaattgc tttttttaaa tagatatcca
    14221 ttttctcatt cccttctgtc ttagtttacg gaaaaaaaat caaaaatacc agaatcaaaa
    14281 tcattttctt ttaaacaaaa atttcaagag tagaaacaaa atggattctt tttttcgaaa
    14341 aatgcgctac acttatagtt gaacgaataa aaaaggagct gaaagtatga attcattaac
    14401 aagcacttat cgtctatcaa atggttacga aatcccggta gtaggattcg gcacatggca
    14461 aacacctgat ggggatgtcg ctgtttcctc tgttaaagaa gcgctggctg caggttatcg
    14521 ccatatcgat actgcccaag gatacaagaa tgaagaaagc gtcggccaag cgattaagga
    14581 aagtggaatt cctagagaag agatattctt gacaacaaaa ttatggaatg caaaccacag
    14641 ctatgaactt gtgatgtctt cttttgaaga atcattgaag aagctccaaa cagattattt
    14701 agatctgttt ttgatccact ggccaaaccc tgttgctttt cgtgataatt gggaacaagc
    14761 aaatgcagat acatggcgag catttgaaga actgtatgaa gcaggtaaaa tcaaagcaat
    14821 cggtgtgagc aatttcttac cccaccatct agatacacta gcaaaaacag caaaaattat
    14881 gccgatggtc aatcaagttt tccttgcacc aggcgaatta cagacaactg tagttgagta
    14941 tgcaaaaaaa catgatatga tcctcgaagc atatagtcca ttaggcactg gtaaaatctt
    15001 cgatgtgcct gaaatgaaac aaatcgctga agcacatgac aagacgattg cacaagttgc
    15061 tttgcgttgg tcgctgcaac acgaattcct tcctcttcct aagtcagtga caccaagcag
    15121 aatcaaagaa aatactgaat tatttgattt tgaattaaca gaagaagaaa tgaaacaaat
    15181 cgatcagtta gatggtgtag tcggcaaagc aaaagaccca gatactaccc aattttaagt
    15241 cgaaaggtga ttttatggat ccaatcgaac gcttgaattc tctctcggaa gaagttaccc
    15301 agacttttca ttccgatttt gtctttttga tcgatgcaga gaaaatccaa cattttcctg
    15361 caagaaactg gacacatgat caaatcatcg aagaactgaa aaaacggttc gatcattcgc
    15421 taatggtcaa accctggcac gaacatgaag tcatttattc acctgaacta cctgtttttg
    15481 ccttgatacc gaaaaaatag aaagaatg
//
LOCUS       DACEKH010000065        15356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEKH010000065 DACEKH010000000
VERSION     DACEKH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.068"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            332..946
                     /gene="gmk"
                     /locus_tag="IW662_001896"
     CDS             332..946
                     /gene="gmk"
                     /locus_tag="IW662_001896"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ0866717.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            951..1256
                     /locus_tag="IW662_001897"
     CDS             951..1256
                     /locus_tag="IW662_001897"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0866718.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1509..3917
                     /gene="priA"
                     /locus_tag="IW662_001898"
     CDS             1509..3917
                     /gene="priA"
                     /locus_tag="IW662_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ0866719.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3932..4423
                     /gene="def"
                     /locus_tag="IW662_001899"
     CDS             3932..4423
                     /gene="def"
                     /locus_tag="IW662_001899"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0866720.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4416..5354
                     /locus_tag="IW662_001900"
     CDS             4416..5354
                     /locus_tag="IW662_001900"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ0866721.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5354..6712
                     /gene="rsmB"
                     /locus_tag="IW662_001901"
     CDS             5354..6712
                     /gene="rsmB"
                     /locus_tag="IW662_001901"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ0866722.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6725..7465
                     /locus_tag="IW662_001902"
     CDS             6725..7465
                     /locus_tag="IW662_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ0866723.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7462..9531
                     /gene="pknB"
                     /locus_tag="IW662_001903"
     CDS             7462..9531
                     /gene="pknB"
                     /locus_tag="IW662_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ0866724.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9696..10595
                     /gene="rsgA"
                     /locus_tag="IW662_001904"
     CDS             9696..10595
                     /gene="rsgA"
                     /locus_tag="IW662_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ0866725.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10608..11258
                     /locus_tag="IW662_001905"
     CDS             10608..11258
                     /locus_tag="IW662_001905"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0866726.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11259..11897
                     /locus_tag="IW662_001906"
     CDS             11259..11897
                     /locus_tag="IW662_001906"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ0866727.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12052..12906
                     /gene="mreC"
                     /locus_tag="IW662_001907"
     CDS             12052..12906
                     /gene="mreC"
                     /locus_tag="IW662_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ0866728.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12910..13419
                     /gene="mreD"
                     /locus_tag="IW662_001908"
     CDS             12910..13419
                     /gene="mreD"
                     /locus_tag="IW662_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ0866729.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13674..15248
                     /locus_tag="IW662_001909"
     CDS             13674..15248
                     /locus_tag="IW662_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866730.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         5149 a         2879 c         3332 g         3996 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt ttcttcatac
       61 tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc ttgcatctgc
      121 gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc tgttcttatt
      181 acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt ttaccaattt
      241 ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa
      301 gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat taatcgtgtt
      361 gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg agagtgagga
      421 taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga
      481 aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg aagctggaga
      541 gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt catatatcaa
      601 caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag gagcaaaaca
      661 agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag atttagcgga
      721 attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg aagaacgaat
      781 gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg ttgtaaatga
      841 tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac attttcgagt
      901 agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt atgatgttaa
      961 aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta gtgattttag
     1021 caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat tcttttgatt
     1081 cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta gtcaatgatc
     1141 cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt caagcacaaa
     1201 aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa atgtaataaa
     1261 gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt
     1321 cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag ttcttttttt
     1381 tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa tccatcgatc
     1441 tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa aaaaggagga
     1501 aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg cagaccgatc
     1561 agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag
     1621 tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg gctatccaaa
     1681 aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta gaccttgctc
     1741 cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt acttacgcat
     1801 tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa tatgataaat
     1861 tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag cgagaagtga
     1921 tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc tggcgccaag
     1981 agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt
     2041 tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa
     2101 atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa gaagaaccaa
     2161 ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg
     2221 gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac cgagtatttg
     2281 accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca
     2341 cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact ggcagcggga
     2401 aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa acggctatta
     2461 tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg
     2521 gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat gatgaatggc
     2581 gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct atttttgccc
     2641 cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt tacaaacaag
     2701 atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt
     2761 gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga gctcaaaaag
     2821 gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag cttccagcta
     2881 ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact ttttctgcta
     2941 atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc ctactgctca
     3001 atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt cttccatgtc
     3061 ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc tgccattatt
     3121 gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat aaaatccgct
     3181 actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat ccgcaagcta
     3241 ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa cagattttac
     3301 aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt gctaaaggtt
     3361 tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc ctgaatttac
     3421 cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca ggacgagcag
     3481 ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa cattacgcca
     3541 tagaattagc taaaaaacaa aattatgagc agttttatca gcaggaaatg catgtacggc
     3601 atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc catcctgaag
     3661 aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa gtgttaagcc
     3721 ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa aaccgatatt
     3781 attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt ttggatgaaa
     3841 ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt gacaacgaac
     3901 cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga tccaccctaa
     3961 tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga caatcgcttt
     4021 attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg ctgctcctca
     4081 agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga aatttgaact
     4141 gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag
     4201 tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc gttattatga
     4261 tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg ccttccagca
     4321 tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc agatcccgga
     4381 agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta tttatgggca
     4441 cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac gagatccagg
     4501 cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg ccgacaccgg
     4561 taaaagaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa atctccggtt
     4621 cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact gcggcatttg
     4681 gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc aatgtccatg
     4741 cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc atgaatgggg
     4801 agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca ggcggaatct
     4861 atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg tttgaaaagc
     4921 ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg gatggacaaa
     4981 aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag
     5041 aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc
     5101 cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta tcggttgaag
     5161 tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc
     5221 tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag ccagctggta
     5281 aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt cagattggtc
     5341 agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac gatatatggc
     5401 attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga
     5461 gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag aattgatcta
     5521 tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga tcgctaaagc
     5581 aaaaaaagtg gatgactggg tcaagacgct tctttatctt tctgtatacc aattagaatt
     5641 tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa
     5701 aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg
     5761 agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct
     5821 gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct
     5881 cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac gccgactttc
     5941 cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa aaagccaagt
     6001 cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat tgttccacga
     6061 tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga
     6121 gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga cgcatattgc
     6181 ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac acgaacacaa
     6241 agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg tctacgctca
     6301 aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg atcggatttt
     6361 agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata ttcgttataa
     6421 aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt tagaaagctg
     6481 cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga tcttaaaaga
     6541 agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg agaggattga
     6601 tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga ctttgtatcc
     6661 gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga
     6721 aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa atcaagacta
     6781 tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag acgggatggg
     6841 tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag gcgaagcttg
     6901 ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc aatcgattca
     6961 agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata acggtatggg
     7021 aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca atgttgggga
     7081 tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg atcattcttt
     7141 agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga accatccaag
     7201 gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag atgtttctac
     7261 tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga cgaacatgct
     7321 gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac
     7381 tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg ttcttcttat
     7441 cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa
     7501 atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca cgatttaatc
     7561 ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga ccagactgcg
     7621 attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc aaacattgtt
     7681 agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga atacgtcaaa
     7741 ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga aacagcagta
     7801 aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca aatcatccac
     7861 cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa aatcacggat
     7921 ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac gatgctggga
     7981 tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa gcagtcagat
     8041 atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc gtttgatggt
     8101 gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc tataaaagca
     8161 cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac ggctaaagaa
     8221 cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac agtattatca
     8281 ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga aacaaaagtt
     8341 atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat gcctctgcca
     8401 aaagacaagc cagaaaacga aaccttgcca gaggaggaag aagttctgcc tgatcctaaa
     8461 aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt tttaggaatc
     8521 ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc tgatgtttct
     8581 gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca
     8641 aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa gacagacccg
     8701 gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag tagcggcacg
     8761 aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga agcaagtttg
     8821 aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag ttcagaggta
     8881 gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga tcctaaaaca
     8941 gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag tagtcatgta
     9001 ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa tatcaataat
     9061 attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat cagtcaggat
     9121 ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt aagccaagga
     9181 acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca aagttattta
     9241 gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa cacggtggat
     9301 aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa aggaatggtt
     9361 gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga aaaagactct
     9421 tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc aaaagaagaa
     9481 tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata agtgctgaat
     9541 aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt gtgtttctat
     9601 ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat aacacctact
     9661 ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa ggacaaatca
     9721 gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat caaacaagag
     9781 gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag gtgatctttg
     9841 agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa aatcaattag
     9901 ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc gtagagcctg
     9961 cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag tcgatcgaac
    10021 cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc gaagagatca
    10081 agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg ggtgatacga
    10141 cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag tctggagcag
    10201 gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca ggagagattt
    10261 ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt cctatctatg
    10321 gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca attgaagcag
    10381 gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc aaatttagag
    10441 agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa acaggtggta
    10501 tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa aaaagaaaac
    10561 caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg aagatagcac
    10621 catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta gtggaaaaag
    10681 gaggcgcaga ttacatccac gtagatgtaa tggatggcca atttgtccca aatatcacat
    10741 tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta gacgtccatc
    10801 tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga gcagatatca
    10861 ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg acaaaaagct
    10921 taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg atcaaacatg
    10981 ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc ggcggacaaa
    11041 gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt agagagaaca
    11101 attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca aaaatctgta
    11161 aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc gaaaatccag
    11221 tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta ttagctgctg
    11281 gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga
    11341 tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg gcagtgggcg
    11401 attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc aaagagtata
    11461 cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta aaaacatttg
    11521 aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga ttggatcatc
    11581 ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc catcagctta
    11641 ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat atagtaagac
    11701 agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg aatcttacat
    11761 taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca tcttatgcaa
    11821 gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg attgcagtta
    11881 ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga
    11941 cagaatctat tatttgctaa aaaaaagctt aagaggagca acttaccttt tgtttcacgg
    12001 catttagtga taaaattgaa cacaagagtc taattaattc gaggtgtctt tgtgaagaag
    12061 ttcaatccta ataaaaatat tattattacc ttggttattg ttatcatcgt ggtgacgata
    12121 ctaagtgtga ccgctgcgca gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc
    12181 gttaatgaca gtgttgcaat ggttgataaa acaatctctg ccccagtacg gtgggtacga
    12241 aatggagtag attctgtcca taatcttttt tcaacttatg ccgaaaatga gcggctaaaa
    12301 gaaaaaatcg atagctatga tgacgtttca cagcaaaaca agaattacga gagagaaatc
    12361 gaagcgttga aaaaagaact tgagttgaat cagacgctga ctagttacga aaaagtaacg
    12421 gcaaatgtca ttactcgttc gcctgacagt tggcaggaca tgctgatcgt agacaagggt
    12481 tctaaagacg gtatcgaagc aaatatggca gtcatgtcgc aaaaaggatt gattggccga
    12541 gtgatcgagg tcaatacggc ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc
    12601 tccaatcatt ttccagtacg ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa
    12661 aactatgatg aaaagctcca tgccttagtg gtgacccaat taactggtga tacggatatc
    12721 aaagaagggg atgttgtcca gacatccggt cttggaggga attctccagc taacttgccg
    12781 atcggtacgg ttattaaaac gaaaccagat agttatgggc tggatcggga agtttatgtg
    12841 aaaccttatg cagaaatgta tgacgtgtca gttgtgacga ttgtccaacg gctagcggag
    12901 gattagtcca tgttgaagaa agaaacaatg aagtactatc tgccaatcgt tttgttcttt
    12961 ttgatgttga tagatggtca tttaacaaga atgctagggg agtggtcgaa aggcacctat
    13021 atgtcaaatg cccactttct gatattggca ttattatgtt gcagtatggc gtttgaaaaa
    13081 cgttatttac tgattaccac gattgttctc ggggctatct atgacgctta ctatattggc
    13141 gttatcggta tctatgcagt agctctccct ttaattgtat ggttgatgta tgtaatgaaa
    13201 gacgttatcc atgtcaacat ctttactgaa tttttcagta tgatcatctt tgtcacgggt
    13261 tatgaattgt ttacgatggt ggtccagttg atttttaaat tagcagtagt aaataacacg
    13321 tattttatta caaggttttt aggacctaca ctgctgttga acatgattat atttgtatta
    13381 ttcatttttc cctttaagaa attattcagt gatgaataat ttcttttttt atttttaggt
    13441 cgaaagcgaa aaagaatcgt tgttatctta agaaatgcta gacttaacgc ggctttttca
    13501 tgatcatttt ctgaaacgaa cagttttgtc atttttaaaa gttgttttga aacatttttg
    13561 taacataact attatatcgt tgacacactg ctatggtaca atttttatat cgttaagaaa
    13621 gacttaaata tatttagata tttattaaat taagccagtc ggaggaatga agagtgaaaa
    13681 agagtttaat atcagcagta atggtatgtt caatgacctt gactgccgta gcatcaccga
    13741 tcgctgccgc ggcggacgat tttgattctc agatacaaca gcaagatcaa aagattgcag
    13801 acttgaaaaa tcaacaagca gatgcacaat cacaaatcga tgccttagaa agccaagttt
    13861 ctgaaatcaa cacacaagca caagacctat tagcaaaaca agatacattg cgtcaagaat
    13921 ctgcacaatt agtaaaagac attgcagatt tacaagaacg tattgaaaaa cgtgaagata
    13981 caatccaaaa acaagcacgc gaagcacaag taagcaacac aagctcaaac tacatcgatg
    14041 ctgtattgaa tgctgattca ttagcagatg ctattggacg cgttcaagca atgacaacga
    14101 tggtaaaagc aaacaacgac ttgatggaac aacaaaaaca agataaaaaa gctgtagaag
    14161 acaaaaaagc tgaaaacgat gctaaactaa aagaattagc tgaaaaccaa gctgctttgg
    14221 aatctcaaaa aggcgatctt ttatctaaac aagctgattt gaacgtactg aaaacttcat
    14281 tagctgctga acaagcaact gctgaagaca agaaagctga cttgaaccgt caaaaagcag
    14341 aagctgaagc agagcaagct cgtattcgtg aacaacaacg tctagcagaa caagctcgtc
    14401 aacaagctgc acaagaaaaa gctgaaaaag aagcacgcga acaagcagag gctgaagcac
    14461 aagctactca agcttcatct actgcacaaa gctcagcaac agaagaatca tcagctacac
    14521 aatcttcaat gactgaagaa tcatcatcag ctacacaatc ttcagcaaca gaagaatcaa
    14581 caacacctga atcttcaaca gaagaatcaa cagcgcctga atcttcagca acagaagaat
    14641 caacaacagc gcctgaatct tcagcaacag aagaatcaac aacagtgcct gaatcttcag
    14701 caacagaaga atcaacaaca gtgcctgaat cttcaacaac agaagaatca acaacacctg
    14761 cgcctacaac accatcaaca gatcaaagtg ttgatacagg aaacggcaca ggaagtagta
    14821 ctccggctcc aacgccaaca ccaacacctg aacaaccaaa acctgtaaca cctgctccag
    14881 ctccttctgg aagtgtgaat ggtgcagcaa tcgtagcaga agcttacaaa tatattggta
    14941 ctccatatgt ttggggcggt aaagatccaa gtggctttga ctgctcaggg ttcacacgct
    15001 atgtttacat gcaagtaact ggtcgtgaca tcggtggttg gacagttcct caagaatcag
    15061 ctggaacaaa aatttcagtt tctcaagcaa aagctggaga cttgttattc tgggggtcac
    15121 aaggcggaac ttaccacgta gcgattgctt taggcggagg acaatatatc catgctcctc
    15181 aaccaggcga aagcgtaaaa gttggttcag ttcaatggtt tgcccctgac tttgctgtca
    15241 gcatgtaaga atgattaatc aaaaagagtt agcccattct ggactaactc tttttttatt
    15301 gtgataaaat agaaatcaga taaattcttc tgtcaattcc atgaacgttt ggatat
//
LOCUS       DACEKH010000066        15111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEKH010000066 DACEKH010000000
VERSION     DACEKH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.069"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IW662_001910"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IW662_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0866731.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IW662_001911"
     CDS             complement(322..891)
                     /locus_tag="IW662_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ0866732.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IW662_001912"
     CDS             1077..1979
                     /locus_tag="IW662_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0866733.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IW662_001913"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IW662_001913"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ0866734.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IW662_001914"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IW662_001914"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ0866735.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IW662_001915"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IW662_001915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ0866736.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IW662_001916"
     CDS             8547..8732
                     /locus_tag="IW662_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ0866737.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IW662_001917"
     CDS             complement(8794..9990)
                     /locus_tag="IW662_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0866738.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IW662_001918"
     CDS             complement(10160..11596)
                     /locus_tag="IW662_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0866739.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IW662_001919"
     CDS             11889..12698
                     /locus_tag="IW662_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0866740.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..15037)
                     /locus_tag="IW662_001920"
     CDS             complement(12884..15037)
                     /locus_tag="IW662_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ0866741.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4418 a         3201 c         2670 g         4822 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag
    15001 aagtatttaa tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac
    15061 attcatgctt cattgtaccc aaaaacttct taataaaaaa gaaaaaatgg g
//
LOCUS       DACEKH010000067        14669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEKH010000067 DACEKH010000000
VERSION     DACEKH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.070"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            9..1013
                     /locus_tag="IW662_001921"
     CDS             9..1013
                     /locus_tag="IW662_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866742.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1014..1958
                     /locus_tag="IW662_001922"
     CDS             1014..1958
                     /locus_tag="IW662_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866743.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            1958..2920
                     /locus_tag="IW662_001923"
     CDS             1958..2920
                     /locus_tag="IW662_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0866744.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            2947..3861
                     /locus_tag="IW662_001924"
     CDS             2947..3861
                     /locus_tag="IW662_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0866745.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3887..5668
                     /locus_tag="IW662_001925"
     CDS             3887..5668
                     /locus_tag="IW662_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0866746.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6016..6702
                     /locus_tag="IW662_001926"
     CDS             6016..6702
                     /locus_tag="IW662_001926"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0866747.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6722..10303
                     /gene="smc"
                     /locus_tag="IW662_001927"
     CDS             6722..10303
                     /gene="smc"
                     /locus_tag="IW662_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ0866748.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10312..11121
                     /locus_tag="IW662_001928"
     CDS             10312..11121
                     /locus_tag="IW662_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0866749.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11133..12131
                     /gene="ftsY"
                     /locus_tag="IW662_001929"
     CDS             11133..12131
                     /gene="ftsY"
                     /locus_tag="IW662_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ0866750.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12318..14585
                     /gene="gshAB"
                     /locus_tag="IW662_001930"
     CDS             12318..14585
                     /gene="gshAB"
                     /locus_tag="IW662_001930"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ0866751.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
BASE COUNT         5008 a         2639 c         3225 g         3797 t
ORIGIN      
        1 gtattcgatt gtccgataac caattattag aagttcagaa cctacataca ggattccgac
       61 tcaaagatga ctattacgat gcagtagatg acgtttcatt tactttagac aaaaacgaga
      121 ttttagcaat cgttggggaa tctgggtgcg ggaaaagtac cttggctact acgattatgg
      181 gattacttga tcctaataac acaaaaatta caggtgagat tttatataac gatctgaatt
      241 taatcgacct gaacgaaacg ctctataata aaatccgcgg aaatgatatc ggaatgattt
      301 tccaagatcc gttgtctgca ttaaatcctc tcatgagaat cgaagaccag atcaaagaag
      361 gactttccta tcacacaaag atgaatgcag aacagcgtca agctcgtgca ctagaattgc
      421 tagaacaagt agggattcct aatcctgcga gagttggacg ccaatatccc catgaactat
      481 caggcggtat gagacaacgt gtcatcatcg caatcgcaat cgcctgcaaa cctccaatcg
      541 tcattgcaga tgaaccgacg acagccctag atgtaaccat ccaagcacag attcttgatt
      601 tactgaaaga ccttcaggaa gaaacaaaaa cagggatcat cttgatcact catgacttag
      661 gagtcgtagc agaaatggct gatcgagtgg cagtgatgta tggcggacag ttcgttgaag
      721 tagcaggtgt aaaagaactg tttgatcatc ctaagcatcc ttatacacgc tctttactga
      781 actctatacc acaagaagga aatcatgatg cagaactcca tgtgatcgaa ggtgtcgttc
      841 cttctctgaa aaacatgcct cgtaaaggtt gtcggtttgc accacgtatt ccatggatac
      901 cagcaagcga tcatgaagaa gagcctatcc ttcatgaagt cgcaccgaac cacttggtac
      961 gttgctcctg ctataagcat ttccacttta gagacgaaaa aggagaggtg tagatgacag
     1021 aattaatcac aatcaaagac ctgaaagttc attaccctat tcgcagtgga ttcttcaatc
     1081 gagtaacgga tcatgtatat gctgtagatg gtgtggattt tattatcgaa aaaggaaaga
     1141 catatggttt agtgggagaa tctggctctg gaaaatctac tacgggaaaa gccgttgtgg
     1201 gattagaaaa ggtgacagcc ggacaaatca tgtatgaagg aaaagatgta acgaagcgaa
     1261 gcaaccgcaa aaaaatgggt tataacaagg atgtgcagat gatcttccaa gattcaatgt
     1321 ccagtttgaa tcctaaaaag cgagtgttgg atatcattgc tgaacccatc cgaaattttg
     1381 aacggttaag cgatcaagaa gaaaagaaaa aggtaaaagg actgctagat atcgttggga
     1441 tgccggaaga tgcgctatat aaatatcctc atgaattttc aggcggacaa cgtcaacgtc
     1501 taggagtagc ccgtgctgtt gctaccaatc caaaactgat cgtagccgat gaacctgtat
     1561 ctgctctgga tctatctgtt caagcgcaag tattgaattt catgaaacga atccagcaag
     1621 aatacggcct cagctattta ttcatttcac atgatttagg tgtagtaaaa cacatgtgcg
     1681 acaatatcgc aattatgtac aaaggtcgat ttgtagaaat cggtacaaga gaagacattt
     1741 ataatgaccc gcgacatatc tatacgaaac gtctgctttc agctattccg caaatcgatg
     1801 tagaaaatag ggaaagccac aaagaaaatc gacgaagagt agaacaagag tatatccaaa
     1861 accaaaaaga atactatgat gcgactggtc gagtctacga tttgcgtacg atcacaacta
     1921 cacacaaagt ggcgttgaaa gatggaggtg caagctaatg tggaaaacga ttctgcgacg
     1981 tgttttattg atgatcccgc aagttattat tttaagtgtt ctgatcttct tattagccca
     2041 agcgatgccg ggggatccat ttaccggttt gatcaatcca aatcaggatc cggcagtgat
     2101 cgaacagatg cgacaagcag ctggcttgaa tgacccatgg tatgtccagt acttccgttg
     2161 gatcggaaat gctttgcaag gcgattttgg tcaaagtttc ttatacaaac gctctgttgc
     2221 tgacttgatc ggcgaacgga tcatcaacac tttatatcta tcgatcttga cagttgtcct
     2281 tacttatttg attgctgtgc ctctggggat gcttgctggc cggtatcaaa attcaatctt
     2341 ggataagatt gtcgtaatct acaacttctt tagttttgct gttcctttat ttatcttcgg
     2401 cttgatcatg ctatttgttt ttggttatcg tttaggctgg ttcccaacaa gtggatcaca
     2461 aacactaggt ttgcaaggtg gatttatcac tcaatggata gaccgtttga aatatattct
     2521 gttaccatca attacccaag catttttggc gactgccgtc acaatccaat atctgcgaag
     2581 tgaagtaatc gattcgaaat cgctcgattt tgtcagaaca gcccgttcca aaggggtacc
     2641 gacgaataaa gtattcagtc gacatatttt ccgaaatgca gcgttgccga tggcttctca
     2701 aatggggtat gaaatcactt cgttgattgc tggttctgta gttatcgaaa agatttttgg
     2761 ttatccggga gtcggaaaac tatttattga ttccatcggc caacgggact ataccgtgat
     2821 cacatcctta gtattgattt taggaatcgc gacgctagtg gggacattac tttcagatat
     2881 catcatgagt atcgttgacc cgcgtattcg gattcaataa aaccaatcag aaaggagaat
     2941 ggatcgatga gtgaaaaaaa caatacagca gttcaagaaa gtattcctcc tatggggata
     3001 cgaatgattg ctcgagaatt taaaaaagat aaaattgcta ttttttcatt aggtttgcta
     3061 gtggttttat tattagtgat ctttatcggt gcgatgctga ccgatcaaga caaagtgatg
     3121 tatgttagta tcttcgacaa atacgctgca ccaggaacag tttcgacaca aggacaaaag
     3181 ttcattctgg gagcagatga aggcggacgt gatgtattag gacaattgat cataggtgct
     3241 agaaactctg ttacgatcgg atttgcgatc acgatcctta cttcgatcat cggggttgga
     3301 ttagggatca tctctggcta ctacggaggg atcattgaca acatcttgat gcggatcgtt
     3361 gattttatca tgattctacc gatcatgctg atcattatcg tcttcgtatc gattatttcc
     3421 agatacaatg tctggtcctt tatattcatc atgagtgcat tttattgggt agctaaagcc
     3481 cgattattcc gaagcaagac attatctgaa gcaagaagag attatgtcag tgcctcaaaa
     3541 acaatgggaa ccaatgattt taaaatcatg ttccgcgaaa tcatgccgaa tctaagttca
     3601 ttaatcatta ctaatttgac gatgaacttt gcggcaaaca tcggtatcga gacaactttg
     3661 actttcttag gtttcggctt gccggcaaat gtacctagtt taggtacatt gatcggttat
     3721 gcaagtaacg gagaagtctt agtgaatcgg caatggatct ggttacccgc atcaatcctt
     3781 attttagttt tgatgttgag tatcaattat gttggacaag cattcaagcg ttctgcagat
     3841 gcgcgccaac ggttaggcta gtatagaagc gaagggggaa ataggaatga agaaaacgat
     3901 ttttggattt gcagcgctga tgtctgctgt cgttcttgca gcatgcgggg gcggaggtac
     3961 agatacctct ggaaacaact ctggaggcaa cggcaataac aaagaggaat taacttttcc
     4021 aattgcaaca aaaaataatg atgatgcaat cgaagacggc gaattagaag ctgcggtcgc
     4081 aacggacagt cagtttaaag gattgttcca gtgggagttt taccaagatt catatgatgc
     4141 agcttttatg gctccttctc atgaaccgct atttgcaaac gatgacgatt tcagaatcac
     4201 agacgacgga gctgcaacgc ttgatctaga tgaagatacg aaaaaagcga caatcaaagt
     4261 aaaagacaac gtcaaatggt cagatggtga agatgtaacg gcggacgatg tgattttccc
     4321 ttacgaaatc attgggaata aagattacac tggtatccgt tatgacgaca ctttccgtaa
     4381 catcgtaggt atggaagaat atcatgatgg aagtgccgac acaatctccg gaatcaaaaa
     4441 aatagacgac aaaacagttg aaatcgaata taaagaagta aacccaagca tgcttcaatc
     4501 gggtggtggt atttggtcat atgcagcacc aaaacatacg ttagaaggtg tagcaatcaa
     4561 agatatggaa tcaagcgatc aagtacgtaa aaatccagtg acttttggcc cttattacat
     4621 gagtaatatc gttgcaggag aatcagtaga atacttgcca aacgagcact actggaatgg
     4681 tacgcctaaa ttgaaaaaga tcacgatgaa atctgtaccg atggcaagtg caactgaagc
     4741 attgaactct aaattatatg atatgatttt ccaaatgcca acagatactt acgataccta
     4801 taaagaaatt gctggctata caaacttagg ccgtgaacaa acttcttata cttatctagg
     4861 attcaagtta ggtaaatgga atgcagatac aaagagtgtg gaatacaatc cagattcaaa
     4921 aatggctgat aaatcattac gtcaagcaat gggctatgca ttagataata atgcagtagg
     4981 tgagcgtttc tatgcaggtt tgagaagcaa tgcgacttca ttgattcctc cagtattcca
     5041 gtcataccat gatgatgaga tgaaaggatt cacacaagat ctagacaaag cgaaaaaact
     5101 attagatgat gctggctaca aagatgtgga tgacgatggt ttaagagaag acaaagatgg
     5161 aaataaacta acaatcaact ttgcttcgat gtccggtggg gaaacagcgc aaccattggc
     5221 agattactat atccaacagt ggaaagaaat cggtttggat gtccaataca caacaggtcg
     5281 tctgatcgaa ttcaattctt tctatgataa attagaaaat gatgatcctg aaattgatgt
     5341 ttaccaagct gcttggaata caggaactga tccaaatcca agtggactat ggggaccaaa
     5401 tgctgcgttc aactacactc gttatgaatc agaagaaaat acaaaattga tcaacgatat
     5461 cagttcaagc aaagcatttg atgaagagta ccaaaaagaa gcatatcaaa aatggcaaca
     5521 atatgcattt gaagaagcat ttgcgattcc aacgttgttc cgtaatgaag tattgccagt
     5581 caacgaccgt gtcaaagact gggattggtc ttatgatgta ccaaacccat gggcaaatgt
     5641 ttctgttaca gcagattcac gttcataatc actaaaaatg accgaaaaag ggcgatgtcc
     5701 tttttcggtt ttttgatatt tttctgttta tttctctaat attcaggtca aggcgctgat
     5761 ttcatgactt ctccaataaa cggtgttcaa agtccagttt ctcggtatta agtcaaattt
     5821 atgcaaaaat atggaaagcc attttcacaa aattttttct tcatactcga aactaaacga
     5881 atttttcaca acttctcgtc aatcactagg attattaaag ctttacttac cttttgaccg
     5941 aaccatttca aatttgacaa aaaaccagta taataaggaa gtatcgaaaa aatatccata
     6001 aaaggaatgt ggaaaatgga caaccagctg acaaaagaac taaaagaaaa ctataacatc
     6061 gtttttcacg atctaaattt gcttgaacaa gcttttaccc attcatccta tgtgaatgag
     6121 catcgcaatc tgcaattatc ggataatgag cgacttgaat ttttaggaga tgccgtttta
     6181 gaactgatgg tttctcagta tttattccgt ttatatcctc atttgccaga aggaaaattg
     6241 acaaaaacac gagctgcgat cgttcgcgaa gacagcttgt cgaaatttgc caaagaatgt
     6301 cactttgatc aatatgtttt gttgggcaag ggagaagaga attcaggagg acgtacgcga
     6361 ccagcattgc tttgcgacct attcgaagct tttttaggcg cgttatatct tgaccaggga
     6421 ttcgacacga cccatgcatt catcgaaaaa gtgatttttc ctaaagtcaa agcaggtgct
     6481 ttttcacatg agatggatca taaaacaaaa ctacaagaag tcttgcaaaa atcaggtgat
     6541 gtatcaatcg aataccgatt gatcaatgaa gaaggtccag ctcatgaacg agtattttgg
     6601 atcgaagttt atgtagatga tcaattgatt ggcactggac aaggaaaatc gaaaaaatta
     6661 gcagaacaag ctgcggcgga aaatgcattg gcagcactgt aaaagtaagt gagggaaagc
     6721 tttgtatttg aaacgaattg aaatagccgg ttttaaatca tttgcagaca agacagtcat
     6781 tgactttgaa aatagcgtca cagcagtggt tggaccaaat ggcagtggaa aaagcaatat
     6841 tacagaagct atccgctggg tattaggaga acaatctgct aaaagtctgc gaggcggaaa
     6901 aatgccggat atcatctttg ccggttccga tacccgtaag cagctgaata ttgcagaagt
     6961 cacagtgatt ttagacaata gtgatcatta tctgccattg gagtacaatg agatcagtgt
     7021 aacccgccgt tatcgacgga ctggggaaag cgagtttttc atcaataaac aaagctgcag
     7081 attaaaagat atccaagaat tgttcttaga ctctggatta ggaaaagaat cgttctcaat
     7141 catttctcaa ggaaaagtag aagcgatttt cagtagtaaa ccggaagacc gtcgtgggat
     7201 ctttgaagaa gcagctggtg tcttgaaata caagcagcgg aaaaagaaag cagaacagaa
     7261 gctttttgaa acagaagaca acctcagccg cgtccaagac atcatccacg aattggaaga
     7321 acagttaact cctttggccg cccagagtga ggcagcaaag gagtttttac ggctaaaaga
     7381 aacactgact caaaccgatg tctctttgat ggttgccgaa atcaagacag caaaaaaaga
     7441 ttgggacaac aaacaagcac agcttgcgaa attcaatcta gaattaggca aactatctga
     7501 gtcgatccaa gaacaagaat ccattttggc gaagcagcgt aaagaaaatg cgcaagccga
     7561 tcgtctgatc gaaaaaaatc agcaagtatt attagatctt tcggaaaaac tgaagcagac
     7621 agaaggacaa aaagatgttt tgcaggagcg gacaaagcat acacaaaaaa gctcgcaaga
     7681 atatcaaact tcattagcag aagcacaaaa aaaggttaaa cattttgaaa agcttcaaga
     7741 aagcttgttg aaagaagtag cagaaaaaga aacagaaatc caaaaagctg aagcaagtct
     7801 cataaaaaca cagcaagaat tagaaaaata ccaaaaatca acgaaagaat tactggcaga
     7861 actaagagat caatatgttg atttgatgca agagcaagca gctgtaggaa acgaactgaa
     7921 gtatctagaa cgtcaatatg tacaagaaac agcaaaaagt aagcagacgt tagccaagca
     7981 atcggaagta gaggcatcag tagaccgatt gatcttacaa aaagaagaat tgacacaaaa
     8041 gcaggcgcag ctaaaatcat ctttaactga aacaaaagag aagttagaaa tgatccaaca
     8101 aaacgggaaa aagttccaag aagcattagc aaaagaacag cctaaaatgt atcaattaat
     8161 gaatcaagtc cagcagctac gtgctcgtaa gaaaagcctt caagaaatcc aagaaaacta
     8221 ttttggtttt taccaagggg ttcgattggt cttgcagcat aaacagcagc tttcgggtat
     8281 tgtcggagca gtggctgaac tgatcgatgt tcctgcagat ttcacgttag ctatcgaaac
     8341 agctttaggt ggggcagccc agcatgtcat cgttgaaaat gaaaaagatg ctcggcaagc
     8401 catcacgtat ttgaaacagc aaagaggagg aagagcaaca ttccttccac tgacgacaat
     8461 caaaccaaga caattaccgg ctcatatatt gacacaagca gcagctgtag aagggtttat
     8521 agggattgcc agtgaacaag tcagttatcc cgatcaaatc caaactgtgg ttcaaaacct
     8581 gctaggaacg atccttttag ccaaagactt aactagtgcc aatgcaatcg cgcaaacgat
     8641 ccgctatcaa tatcgagtgg tttccttaga aggtgatgtg atgaatgccg gaggatccat
     8701 gacaggtgga gcgaataaac gaggaaacca aggcagtttg tttgtacaaa atcaagaatt
     8761 gaaacagttg actagtgaat ttgaagaagc agataaacaa ctccaagcac aagaaaaaaa
     8821 agttcaggag ctgcaacaag aaacagctcg tttagccgaa gaacaagaag ttttgcgtac
     8881 acgaggcgaa caattacgtt ttgaagaaca agaagcgaca aatcagctgc aaaacatcat
     8941 aaatgaactt gaacgttttg aaaaagaaaa gcaaatctca acttttgaga cacgtgaact
     9001 gcagcagttt atcgaagatt acgaaaagca aacgaacgaa ttgaaagaca aacaaacaga
     9061 tcttgaatcg caacgacaaa aaatcgacga agaaatcaaa tcgttaagtc aagaaagcga
     9121 ccagatggaa gccagaagag cacaagtaca aagtcaaaaa gcacaagaac aagcagatct
     9181 tgctgtcttg aaagagcagt ttaatcatct gcagatccag ctccgcggtg cccgagtcca
     9241 aaaagcagag gcaaccgaac gtcaagaagc gatagaaaag cagttggcga cattgacagc
     9301 agacttttct gatcatgaag tcacagaaga aagtttggaa aaacaaatta acgaattatc
     9361 tgctcagcga gaaacattga aagccgagtt ggcaaaagcc aaagagcagc gagatagaag
     9421 gcaaaaggag atcgataagc ttgaagcagt cttggcagag cgtaatcgtg agcaaaaagc
     9481 acgactgtcg gaacaatcca aattagaagt ccaaaaagac cgagctgaaa tgattttaga
     9541 taatcatttg acctatctgc agtcagaata tcagctgaca tttgagaaag cttcccaaga
     9601 ctatcaagaa acaaccgata tcgaagacag tagaacaaag gtttcatcct taaaagaaca
     9661 gattgaaaaa ctgggaccag taaacttaaa ttcaatcgaa caatacgaac aagtcagtga
     9721 aagacataca ttcttagcaa cacagcgaga tgatctgtta gcagcgaaaa accaattgtt
     9781 tgaaacgatg gatgagatgg atgatgaagt gcggacaaga ttcaaagaag tatttgaagc
     9841 tatccgtcaa gaattcaaaa ttgtctttcc aaatatgttt ggcggcggta aagctgaatt
     9901 agtactgaca gaaccaactg atttgctgaa aacaggtatt gagattgaag tacaacctcc
     9961 tggcaaaaaa ctgcaaagtt taagtttgct ttctggagga gaacgagcac ttacagccat
    10021 cgcgttgctc ttttctatta tccgtgtctg tcctgttccg ttttgtatct tagatgaggt
    10081 agaagcagcg ttagatgaag ccaacgtgaa acgttttggt cgttacttga gcgatttcca
    10141 agatgacaca caatttatcg tagtcacaca ccgcaaagga acgatggtcg cagcagatgt
    10201 actgtacgga gtgacgatgc aggaatccgg tgtatcaaaa atcgtttctg tacggatgga
    10261 agatatcaat gaagaggcga aaatcaacga aacgaaagtg taggaacgaa aatgatcaaa
    10321 ttagtagcaa tcgatttgga cggtacatta ttaaatgaac aaaagctgat ttcagatgag
    10381 aataaacaag cattagcaca agcaaaacag caaggggtca agatcgttct atgtacagga
    10441 cgccccttag ctgcgatggt gcattatctg caagaattag gactggtaga tgaaggtgac
    10501 tacagcatca ctttcaacgg cggtttggta caaaaaaatg atactggtga aatcatggaa
    10561 aagaaagtga tggaagtatc agatatccaa cgcttataca aactggctca gcaattggat
    10621 ctgccactag atgttttgtc tgacaatgtg gttttacagc tgcctagtgc cccaaggaag
    10681 caatccttgt acaatgtgtt gaataagctg ctgcaattcc aaccagcgga tataagcgat
    10741 attacagatg aatttgtgct taataaggca gtaatcgctt atgaccaaga agaattagat
    10801 cctaaaatca aagaaatacc agcagaattc catgaaatgt atgagatcat caaaacgaga
    10861 agtatgcttt tggaatttat gccaaaaggt gttacaaaag cttatggtat ttctttattg
    10921 gcaaaagatc ttggtttgaa acaagaagaa atcatggcaa tcggtgatga agaaaatgat
    10981 ttaccgatga tccaatacgc tggactaggc gtagcgatgg agaatgctgt gcctttcgtc
    11041 aaagaagcag cagatttcgt gacagccagc aatgaagaaa atggcgtagc caaagtgatc
    11101 caaaaatttg tattagagta ggggggagtc aaatgggatt ttttgataaa atcaagcgtg
    11161 cttttactgg cgaagagaaa acagaagaag ttgaagtaca ggagaagtac gacaagggtt
    11221 tagaaaagac gcgtaaaaca tttggtgaac ggatgaatga gttgtttgcc aattttcgaa
    11281 cagtcgatga agactttttt gaagaattag aagaaacact tatcggtgca gatgtaggtt
    11341 ttgaaacagc gatacgaatc accgaagcat tacgtcaaga agtgaaactc agaaatgcga
    11401 aaaaatcggc agaagtccaa aatgcgatca tcgagaaact tgtggatctc tatgaagcgg
    11461 aaggaatcaa tgaagtaaat gaactaaata tccaagaaga cggactaaca gttattttgt
    11521 ttgtcggtgt taatggtgta gggaaaacaa caagtattgg taaacttgcc catcagttca
    11581 aaaacgaagg taaaaaagta ttgatggcag cagcagatac cttccgcgct ggagcaatcg
    11641 accagttagt tgtctggggc gaacgcgctg gtgttgaagt tgttcgtggc aatgcaggcg
    11701 gcgatccagc agctgtcgta ttcgatgctt tggaaagagc aaagatagag caggcagatg
    11761 ttttactagt tgatactgca ggccgactgc aaaataaagt gaacttgatg aacgaattag
    11821 aaaaaataaa acgagtaatc cagcgggaat taccagaagc accccaagaa acacttcttg
    11881 tactagatgc aacaactggt caaaacgcga tggttcaagc aaaacaattc aaagaaacaa
    11941 cagatgtgac cggtcttgtt ttgacaaaat tagatggtac agcaaagggc ggtatcgtac
    12001 ttgctatccg taatgaattg catttaccag tgaaattggt cggtcttggt gaaggaatcg
    12061 atgacttgga gccgtttgat ccaaatgatt ttgtcgttgg attgttcaaa ggattactaa
    12121 aagaagaata acccatgtct aaaaagcagg agctgtgaca aaagttgcag ctccttgttt
    12181 gaatgattgt ttttaacata gctgtgtttt tgatgccttc atttttatac ttatttgtaa
    12241 gcggttctca aacaatttcg ttctttttct tagacttttt gattataatt agattcatat
    12301 gaattggagg tcacttaatg atgaatttta agcaattatt attgcatgtc aatgcgcgcc
    12361 cttttatcga ccaagctcga tttggtatag aacgggaagg acagcgggtt gatcttgcag
    12421 gaaatctagc aaaaaccgat catccagcaa tctttggcga tcgatcctat cacccctata
    12481 tccaaacaga ttttagcgaa acacaaacag agatgatcac tcctgttacc gattctattc
    12541 ccgaattatt tcagtatctc gctgctgttt atgatgtgac tgctcgttct ataccgaaag
    12601 aagagatgat ctggccatta agtatgccac ctgccttacc ggaaaaagac gaagcgatca
    12661 ttattgcaaa attaaaaaat ttcgaagatg tcttgtatcg acgttattta gcaaaagaat
    12721 acgggaagcg aaaacagatg gtaagcggta tccatttcaa ttttgaattt ggcgatgagt
    12781 tgctaagaac attgttcagc catcaggaag aatttcaaga tttttccgaa ttcaaaacag
    12841 aactttattt gaaaacggcg agaaacttta tgcgctatcg ctggatgatc acttatttat
    12901 tcggtgcttc accaatgagt gagaagaact actttttaga tgaatcacat ccgcaagaac
    12961 ctgttcgcag tattcgtaac agtgcgctgg gctatacgaa tcatccaaat gtgaaagttt
    13021 cctatgcttc tatgaaacag tatttagcag atatcgagcg aatgatcgaa gaagggaaac
    13081 tttcggagga gaaagaattt tatacaccgc ttcgtttccg aggcggaaag aaagtcgcag
    13141 atttagcaac aacaggtgtt cgttatattg aattgcggaa tatcgatttg aatccttatg
    13201 caagattggg tatcaatcca gagcaagttc ggttcttaca attattcctg atgtacatgt
    13261 tatggacaga agaaaaagaa gactgtgacc aatgggtagc agaaggtacg actcgaaata
    13321 acaaagtagc actagaacaa ccatcagatc aaacagaatt tcatcaagaa ggtagagaaa
    13381 ttcttgaagg gatgaagcaa atgctggttg aattggattg gttggattct ctttatttag
    13441 tagaggaagc attgactcaa atggatcatc ctgaacaaac gttagcagcg aaactctatc
    13501 aagaagcaca gctatcgagt cagcaagaag ttgctgtggc attgggacat caatattata
    13561 aagaaagcca tgaacgtccc tatcagttgg ccggttttcg cgaaatggaa ctttctactc
    13621 agatatttat gttcgatgcc atccaaaaag gtgtccaagt caaagtatta gatgagtcgg
    13681 atcaattttt gcgtctgcaa ttccaagacc atgtagaata tgtgaaaaat gcaaacatga
    13741 caagtaagga cagctatatc gtcccactta tcatggaaaa caaaacagta acgaaaaaag
    13801 tcttaaaaga agctgggttc cgggtaccag gaggcgcaga attttcatcc atggaagaag
    13861 cggtaaaagc ttatccgaga tttgccgagc aggcatttgt gatcaaacct aaatcaacca
    13921 attacgggtt aggtatcacg attttcaagg aaggagctag tctggaagat tatcaagcag
    13981 gtttagccat tgcttttcgt gaagacagtt cggttttagt agaagaattt atgccgggaa
    14041 cggaatatcg tttctttgtg atcgacggag aagtccaagc catcatgttg cgagtccctg
    14101 ctaatgttat tggtgacggt atccgaacag tgaaagaact tgttgaagaa aaaaatagtg
    14161 accctttgcg tggaacgaat catcgtgcac ctttagaatt gatccaatta ggtgaattag
    14221 aacaattgat gttgaaagaa caagggttga cgatcgaatc agttccccaa gccaatcaaa
    14281 ttgtttatct aagggagaac tcaaatatca gtacaggtgg tgattcgatc gatatgactg
    14341 atgaattttc tgaagcttac aaaaaaatcg ccgtttctgc ggtagaagca ctaggagcta
    14401 agattagtgg tatcgatttg attataccag ataaagaaat cgatcctaca actgataaaa
    14461 aagcatatgg gatcatcgaa gcaaatttca atccagccat gcatatgcat gtttatccct
    14521 ttgcaggaaa aggcagaaga ttgacgatga atgtattgaa attgttatat cctgaagtat
    14581 tttaaaagaa aaagcttttg acagattgtc ctatttgatg tcaatctgat caaaagcttt
    14641 tttgcatgta aaaatttatg tgttttttc
//
LOCUS       DACEKH010000068        14546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEKH010000068 DACEKH010000000
VERSION     DACEKH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.071"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..813
                     /locus_tag="IW662_001931"
     CDS             1..813
                     /locus_tag="IW662_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ0866752.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            810..1610
                     /locus_tag="IW662_001932"
     CDS             810..1610
                     /locus_tag="IW662_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ0866753.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1771..3039
                     /gene="rseP"
                     /locus_tag="IW662_001933"
     CDS             1771..3039
                     /gene="rseP"
                     /locus_tag="IW662_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ0866754.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3107..4816
                     /locus_tag="IW662_001934"
     CDS             3107..4816
                     /locus_tag="IW662_001934"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ0866755.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5028..9386
                     /locus_tag="IW662_001935"
     CDS             5028..9386
                     /locus_tag="IW662_001935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ0866756.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9531..10004
                     /gene="rimP"
                     /locus_tag="IW662_001936"
     CDS             9531..10004
                     /gene="rimP"
                     /locus_tag="IW662_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ0866757.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10027..11202
                     /gene="nusA"
                     /locus_tag="IW662_001937"
     CDS             10027..11202
                     /gene="nusA"
                     /locus_tag="IW662_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ0866758.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11224..11517
                     /locus_tag="IW662_001938"
     CDS             11224..11517
                     /locus_tag="IW662_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ0866759.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11514..11825
                     /locus_tag="IW662_001939"
     CDS             11514..11825
                     /locus_tag="IW662_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ0866760.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11838..14144
                     /gene="infB"
                     /locus_tag="IW662_001940"
     CDS             11838..14144
                     /gene="infB"
                     /locus_tag="IW662_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ0866761.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14171..14518
                     /gene="rbfA"
                     /locus_tag="IW662_001941"
     CDS             14171..14518
                     /gene="rbfA"
                     /locus_tag="IW662_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ0866762.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4957 a         2583 c         3237 g         3769 t
ORIGIN      
        1 atgttacgtt tttttccgca aaaaaacaaa tatattcaag aaaaaagcga atttcaattt
       61 aataccgatg gaccaattcc tcagcacatt gccatcatca tggacggcaa tggtcgttgg
      121 gcgcaaaacc gtcgtttgcc acgagttgct ggccataaag aaggcatgga aacagtaaag
      181 aaagtcacaa aaaaagcatc aaggcttgga gtgaaagtgt tgacgcttta tgccttttcg
      241 actgaaaact ggaagcgacc aaaagatgaa gtaagttttc tcatgcaact acctgtcgac
      301 ttctttgata cgttcgtacc agaattgatt aaagaaaatg tcaaagttca tgtcatgggt
      361 tatgaaaacg tattgccaga acatacgcaa gatgctgtaa gacgtgcaat tgagcaaaca
      421 aaaaacaata ctggaatggt attgaatttt gctttgaatt atggaagtag agcagagatc
      481 gtgactgctg tccaagaaat tgccgaagaa gtagcaaaag gagaaataca cgcagaagaa
      541 atcgatgatg aattgattgc taagcattta atgacaggtt tcttgcctaa agagttacaa
      601 gatcctgagc tgatgatccg tacaagtgga gaagagcgaa ttagtaattt tctgttatgg
      661 cagatcgcat atagtgagct gtattttaca aaagcgcttt ggcctgattt tgatggtgcg
      721 catcttgaag aagcaatcgc ctcttatcaa aatcgagacc gtcgattcgg cggtgtaaaa
      781 aaaacagaag atgagaaagg ggatcagtca tgaaacaacg tgtgatcaca gcagctgtgg
      841 cgctagtctt gtttattccg attatatgga tcggaggcat agcggttgag cttacagcag
      901 caatcttagc ggtagtagga gtttacgagc tgttccggat gaaaggattg acactcttaa
      961 gttttgaagg agttttatcc gccgttggtg cagtgttact tgttttgcca aaagaaagat
     1021 ggtttttctt tcttccagag aatgtaagta catttatgtt attttatctt atagttatga
     1081 ttcttttagg aacatcagtt atttctaaaa atacgtatac gattgatgaa gcaggttttc
     1141 ctgtgcttgt cagcttgtat gtgggaatag gtttccagaa ttttgtcaat gcacgaacag
     1201 aaagcttggc tgttttgata tttggtctat ttattgtttg ggcaactgat atcggagcat
     1261 atatggttgg tcgacgttat ggtcagcata agttaagtcc tgatatttcg cccaacaaaa
     1321 cgattgaagg tgcgctcggt ggtatcggta gtgctgttgt agtcgcattt ctttatttat
     1381 tgctataccc agcaaaagaa ctattcaatc atggtatgtt tgtcatgctt ctgatgacga
     1441 ttattttttc agcagtcgga caatttggtg atttggtcga atctgcaatc aaacgtcatt
     1501 atgatgtgaa agactcggga aatatcttac caggccatgg tggaatctta gaccgtttcg
     1561 acagtttgct gtttgtattt cctatcatgc atttatttgg cattatttag taagtatacc
     1621 tgcgtttctt tgtgaatttc ataaaagacg caggttttta tttttcatta tattgatact
     1681 attggtgtct ttggtatact atggtaaaat tatagtgttt tatcgttgtt ttataaaaaa
     1741 cacaaaaaac aagtaaagga gaaaaaagct atgaaaacga ttctgacatt tatcatcgtt
     1801 tttggtatat tagtgattgt tcatgagttt ggtcatttct tctttgcgaa acgatcagga
     1861 atcctcgtcc gcgaatttgc tatcggtatg ggtccgaaaa tttatgggca tcaagcaaaa
     1921 gacgggacaa catatacgct tcgcctatta cctattggcg gttatgttcg aatggcaggc
     1981 aatggggatg atgaaacaga aatggcacca ggaatgcctc tttctctttt actaaattct
     2041 gacggcatcg tagagaagat aaatttaagt aagaaaatcc aattgacaaa tgctatcccg
     2101 atggaattga gccgttatga tttagaagat gagctgacta tcactggtta tgtgaatgga
     2161 gacgaaacag aagtagtaac ctaccctgtt gatcacgatg cgacgattat tgaaaacgac
     2221 ggaacagaaa tcagaattgc gccaaaagac gtacagttcc aatcggctaa attgtggcag
     2281 cgtatgctga ccaactttgc tggacccatg aataacttta ttttggcaat cgtgttattc
     2341 atcattctgg catttatgca aggtggtgtt caagtgacga acactaatcg cgtaggagag
     2401 atcatgccta atggagctgc ggcagaagca ggcttaaaag aaaatgatga ggtagtcagt
     2461 gtggacggaa aagaaatcca ttcatggaat gatctgacaa cagtcattac gaaaaatcca
     2521 ggtaaaacat tagactttaa gatcgaacga gaaggtcagg tgcaatctgt cgacgtgaca
     2581 ccgaaatctg tagagtcaaa tggcgaaaaa gttggccaac ttggtataaa agccccaatg
     2641 aatacaggtt ttatggataa gatcattgga gggacgcgtc aagctttcag cggttcattg
     2701 gaaattttca aagccttagg atcactattt acaggtttca gtttagataa attaggcgga
     2761 cctgtcatga tgtaccaatt gtcttcagaa gcagcaaatc aaggtgtaac aactgttatt
     2821 agtttgatgg cgcttctttc aatgaatctc ggaatcgtca atctgcttcc gattcctgcc
     2881 ttagatggcg ggaaattagt cttgaatatt tttgaaggga ttcgcgggaa accattaagt
     2941 caagaaaaag aaggaatatt gacattagct ggctttggat ttttaatgtt gttgatggtg
     3001 ctagttacat ggaacgatat tcaacgattc tttttctaga aaaagttgac aaatagggta
     3061 taataacaaa cgataactat tttgaggaaa taaaggagta tatagaatga gacaatcaaa
     3121 aatgttgatt ccaacattac gcgaagttcc aaatgatgca gaagtattaa gccatcaaat
     3181 cttgctaaga gctggatata tccgccaagt atcagcgggt atttattcat atctgccatt
     3241 agcaaatcgt gtattggaaa aactgaaacg cattatgcgg gaagaatttg aaaaaatcgg
     3301 agcggtagaa atgctgatgc ctgccatctt gccagcagaa ctttgggaag aatccgggcg
     3361 ttacgaaaca tatggaccaa acttgtatcg tttcaaagac cgcaatgatc gaaaaatgat
     3421 ccttggacca actcacgaag aaacttttac tgatttgatg cgtaatgaaa tcaattcata
     3481 taagaaatta cctttaaatc tttatcaaat ccaacctaaa tatcgtgacg aaaaacgacc
     3541 tcgcttcggt ttgcttcgcg gaagagagtt tatcatgaaa gatgcatatt ctttccatgc
     3601 aacggaagaa agtttggacg agacatataa agattacgaa aaagcctata ccgaagtatt
     3661 caagcgttgt ggcttagaat ttcgctcgat catcggagac ggcggcgcaa tgggcgggaa
     3721 agactcaaag gaattcatgg cgatctcaga aatcggggag gatacgatct gttattcgac
     3781 tgaaagtgat tatgctgcaa acttggagat ggctacaagc tactatgtat caaaaaaatc
     3841 gcatgaaaca cagcttgaat tggagaaagt agctacacct aacgtaaaat caattgacga
     3901 agtagcagat ttcttttctg ttgaacctca aaaaatcatc aagtctgtat tgttcattgc
     3961 ggatgaacaa cctgtccttg ttttggtacg tggagaccat gaagtcaatg acgtaaaact
     4021 gaaaaacttt ttatctgctg actttttaga agaagcaaca gaagaagaag cacaaaaata
     4081 tctaggtgct gagtttggtt ctgtcggacc agtcggtgtt tcagatgacg taaaagtata
     4141 tgcagaccgt tatgtccaag atttagcgaa tgccattact ggagcgaacg aaacaggatt
     4201 ccactataaa aatgtgaatc cagaacgcga tttcactgtg ctttcttatg aagatcttcg
     4261 ttttgtacaa gaaggagatc cttcaccaga caacaacggt gttcttgcct ttactcgagg
     4321 aatcgaaatc ggacatatat tcaaaatcgg cactcgttac agtgaatcta tgggagcaac
     4381 tgtactagac gaaaatggac gagaaaaatt tgtgattatg ggctgttatg gtatcggtgt
     4441 aagtcgttta ttatcagcaa tcgtagaaca acattcagat gaacaaggaa tcaattggcc
     4501 aaaaggaatt gcgccatttg atctgcatat cgtccaaatg aatctaaaag acgaatacca
     4561 aacaagcctg acgaatgaac tagaagaagc gatgacaaaa gctggctatg aagtactggt
     4621 tgatgacaga aatgaacgcg caggagtgaa attcgcggat tctgatttga ttggctgtcc
     4681 aatcagaatc actgtcggta aaaaagcagt ggatggaatc gtagaagtga aaatcaagaa
     4741 aactggcgaa atggttgaag tacgtaaaga agaattggcg aatactttac caatcctctt
     4801 gaaccaaggc gaataataag cgttaaaaca cagaaaataa aaatagctga atcagctgtt
     4861 tttgacaatc tgatgattcg cttttaaaat tggaactgcg atttgaatcg cagttccaat
     4921 tttttaatgt aaaactcaga aaaaacgagt atactaaaag gtactgaaaa agaagaggat
     4981 aagatgcgat caattgcatt gtttctcatc agaaagggcg tatatctttg tcagaaaaac
     5041 gagatttatt tgaaaaactg ttggaacaaa tccaactaga ggaagcagaa aaaaatcatc
     5101 cactgcttac tgctggtgaa atggatagtg tagtggtcca tcgtaaatcg cgtttatggg
     5161 aatttacgct acatttttcc aaaattttgc ctattatgct atatcggtca ttgactcagc
     5221 atctgactat cgcttttaaa gatattgcac aagtcaaact gaatatctta gcagatgacc
     5281 agacattcga tgaacagctc ttgcaagatt actgggccca agctttggaa aatcagcaat
     5341 gcgatacacc actagcacag caagtattga aaacgcaggt acctgtgata aaagatcgta
     5401 aagtcatctt gccaatcgac agtacaggag ctatttctta tttgaagcaa caatacttgc
     5461 cattagtaga agagttgttc gtcagttacg gattcccaaa atttcgtatc gaaccagaag
     5521 tggatgagca gcaagcggaa cgagtattga aactttttga agaacgcaaa caagaacagg
     5581 cagaagcctt tatgaagcaa gcagctgaaa gtctagttgt ccacgaacaa aagaaaaagg
     5641 aaagaaaaga gcaatcgcca gctcttgaag gacccatcca catccagcta ggacgaaata
     5701 ttccagccga tgaaccaacg acaccaatga tcagtatcgt tgaagaagaa cgaagagtca
     5761 cacttgaggg gtacgtattc gacaaagaag tcagagaact acgttcaaaa cgcaagatct
     5821 tgactttgaa aatcacagat tatacttcat catttatcgt caaaaaattc tcgaacggcg
     5881 aaaaagatga acaagtattt gatgcgatca gtgtcggtag ctggttaaag gtacgaggaa
     5941 gtattcaaga agatacgttt gtccgtgatt tggtcatgaa tgcgcaagat atcatagaag
     6001 tcaaacatac acctcgcaaa gattatgctc ctgaagggga aaaacgagta gagcttcatg
     6061 ttcatagcaa tatgagtacg atggatgcaa caaacagcat ctctgatctt gtggcacaag
     6121 cagggaaatg gggacaccga gctatcgcca ttacagacca cggtggtgcg caagcctttc
     6181 cggaagcaca tagtgctgga aaaaaagcag gtgtgaaaat cttatacggc gtggaagcga
     6241 atgtcgtaga tgatggtgtc ccaattgctt ataatgatgc acatgaagca ttgagtgaag
     6301 ccacatatgt cgtatttgac gtggaaacga caggattgtc tgcagtatat gacacgatca
     6361 tcgagctagc cgcagtcaaa atgtataaag gaaatgtgat cgaaagcttt gatgaattta
     6421 tcgatccagg acatcctttg tcacgaacaa cggtagatct gactggtatc acggatggaa
     6481 tggttcgagg atcaaagtcg gaagaagaag tattgcggat gttccttgaa ttttcaaaag
     6541 atacaatttt ggtagcccac aacgcggctt ttgatatggg gttcttgaat actagctacg
     6601 caaggtatgg tattcctgaa gcggccaatc cagtcattga tacgttggaa ttagctagat
     6661 atctgtatcc tcaattcaaa cgtttcggat taggtgtgtt atctaaaaaa tttggtgtca
     6721 gcttggaaca gcatcaccga gcaatctatg atgctgaagc aactggtcac ttagcatgga
     6781 tctttgtgaa agaggcaatg gacaatcata acatgcttta tcatgatcag ctaaatgagc
     6841 atatcggaga aggagattct tacaaaaggg ctcgaccttt ccatgtaacg attttagcaa
     6901 aaaatcaggc aggactgaaa gatttgttca aactgatttc aatgtcgaat gtcgaatact
     6961 tcgaacgcgt cccacgaata ccacgctccc aattgaaaaa gatgcgtgaa aatctattga
     7021 tcggctcagc ttgtgataag ggagaaatat tcgaagccat gatgcaaaaa ggggtagaag
     7081 aggcaagaaa ccgagcaaaa ttctacgatt atattgaagt tatgccaaaa gcggtctatg
     7141 cgcctttgat cgagcaagaa ttggtaaaaa atgaacatga tttggaagaa attatccaaa
     7201 atttggtaga aatcggaaaa agtttggata aaatcgttgt tgcaacaggt aatgtacatt
     7261 atctgaacga agaagatgcc atttaccgaa aaattttgat caattctatg ggcggagcga
     7321 atccgttgaa tcgtcatagt ttgccggatg tccatttccg aacaaccgat gagatgctaa
     7381 ctgcatttca ctttttaggg gaagaaacag cgaaagagat cgtagtagaa aatacgaata
     7441 aaatcgcaga tatctgcgaa gaagtgattc cagtcaaaga tgaactttat acaccgaaga
     7501 ttccaggttc agaagatgaa atatcagaat tgagttacac aaaagcaaaa caaatgtacg
     7561 gagatccgtt acctgagatc attcaaaaac gattaaaaaa agagttgaat tctattaatg
     7621 gtaatggctt ctcggttatt tatctgatcg cccaaaaact agtgcataaa agtaatgaag
     7681 atggctatct agtaggttca cgtggctctg ttggctctag ctttgttgcg acgatgacag
     7741 gaatcactga agttaatccg ttagcccctc attattattg tccagagtgc caatattccg
     7801 agttttttga agatggtacg tatggttctg gatttgacat gccagaaaaa caatgtccaa
     7861 aatgtggtgc acgcttaaac aaagacggtc atgatattcc atttgagaca tttttaggat
     7921 tccatggaga caaagtgccc gatattgatt tgaacttctc aggtgattat caagcagaag
     7981 cccataacta tacgaaagta ttgtttggcg aagactatgt ttaccgtgcc ggaacgattg
     8041 gtacagtcgc cgacaaaaca gcatatggct atgtaaaagg atacgaaagg gacaataatc
     8101 tgcagtttcg aagtgcagaa gtcgacagat tagcaaaagg cgctactgga gtcaaacgga
     8161 caaccggcca gcatccaggg gggatcatcg ttattcccga ttatatggat gtctatgatt
     8221 ttacgccgat ccaatatcca gcagacgatc aaaattcgga atggaaaacg actcattttg
     8281 attttcactc gattcacgat aatgtattga aattagatat tctagggcat gatgacccaa
     8341 ctgttattcg gatgcttcaa gatttatcag ggatcgatcc acaaacgatc cctacggatg
     8401 acccggaagt gatgcggata tttgccggtc ctgaagtact tggagtaagt caggaacaaa
     8461 tttattccaa aacagggaca ttaggtattc cagaattcgg cactcgtttt gttcgaggga
     8521 tgctagaaga aacgcatcct acaacttttg cagaactact gcaaatttcc ggactatctc
     8581 atggtacaga cgtatggtta ggaaatgcgg aagaattgat tcgacgagga gatgcgacac
     8641 tggcggaagt aatcggctgt cgtgatgata ttatggttta tttgatccat gctggattag
     8701 atagcgggat ggcatttaaa atcatggaaa ctgttcgtaa aggacaatgg aacaagatcc
     8761 ctgatgaatt gcgagaaacc tatctatcgg cgatgaaaga aaataatgtg cccgactggt
     8821 atatcgattc ttgttcaaaa atcaaatata tgtttccgaa agcccatgct gcagcttacg
     8881 tattgatggc tcttcgtgtc gcttatttca aggtttattt ccctattttg tattactgtg
     8941 catacttttc tgtacgtgca gacgactttg atctagtctc catgtgcaaa ggaaaagatg
     9001 ctgtgaaaca agcaatgaaa gaaatcacgg acaaaggatt agatgcttcc gtaaaagaaa
     9061 agaatcaact gacagtgctg gagttagcca atgaaatgct ggaacgagga ttcaaatttg
     9121 gaatgattga tttgtataaa tcggatgccg ttaactttgt tattgaaggt gatacgttga
     9181 tcgcaccatt tagagctgtc ccaagtttgg gaaccaacgt ggcaaaacaa atcgttgaag
     9241 caagaaaaga cggtccattt ttatccaaag aagatcttgc cacccgcggt aaagtttcaa
     9301 aaacattgat cgaatatatg aacgacaatg gcgtattgaa agatctacca gatgaaaatc
     9361 aattatcgtt attcgatatg ctataagcat ctgttttcct gtctgcttgt aattttaagc
     9421 agagtatggt atagttctta taggtaatga tactaactgt gagtgagcgg atttttcgct
     9481 cactcttttt aatggaatta cgcacttaat tttttaagga ggcgaaactt ttgagtagcg
     9541 tcgttgaaac agtcacagaa atggtgacac cgattttaga tgaacaaaat tttgaacttg
     9601 tcgaagtcga atttgtaaaa gaaggaaaaa gctggttctt acgagtattt attgacaaag
     9661 aaggcggtat cgatatcgaa gaatgcgcct ttgtcagtga gaaactgagt gaaaaacttg
     9721 atgcagcaga tccagatccg attcctcaag cttatttcct ggaggtatcc tccccgggag
     9781 cagagcggcc attgaaaaaa gaaagcgact atgaaaaggc acttggtgat tatgtccatg
     9841 tctccttata ccaaccagta gatggcgaaa aacaatatga aggatttctt caatcatttg
     9901 atgctgaaca gcttacattg aaaatacgca taaaaacacg agaaaaagaa ttgacttttg
     9961 accgaaaaaa tattgcaaaa gctcgcttag ctatccaatt ttaatcacgg agaggaatca
    10021 gaaaaaatga gtaaagaaat gttaaatgcg ttagacgctt tagaagctga aaaagggatc
    10081 tcaaaagaaa tcgtgatcga tgctttagaa gcagcattgg tttctgcata caaacgtcac
    10141 tatggccaag cacaaaacgt cgaagtggaa tttgatcaga aaaaaggaaa gatccatgtc
    10201 tatgcggtaa aagaagtaac ggaagaagtg atggattcac agttggaagt ctcattgaaa
    10261 gatgcacttt taatcaatcc agcatatgag atcggtgata cgatccgttt tgaagtaaca
    10321 ccaaaagatt ttgggcggat cgctgcgcaa acggctaaac aagttatttt acagcgtgta
    10381 cgtgaagcag aaagaacgat tatttataac gaattcagtg cttatgaaaa agacatcatg
    10441 caaggaatcg tcgaacgcca ggataaacgt tatatttatg tcaatcttgg taaaattgag
    10501 gcagttcttt ctaaacaaga tcaaatgcca aatgaatttt atcaaccaca tgatcgaatc
    10561 aaagtttatg tttcccgagt agaaaatacg tctaaaggtc cgcaagtatt tgttagccgc
    10621 agccatccag acctattgcg ccgcttattt gagcaagaag taccagaagt ctatgatgga
    10681 atcgttgaaa ttgtcagcat cgcccgtgaa gctggcgatc gttcaaaagt ggcagttcgc
    10741 tcaaatgatc caaatatcga tgcagtcggt acatgtgtcg ggccaaaggg acaacgagtt
    10801 caagcaatcg tcaatgaatt aaaaggcgaa aacatggata tcgtcgaatg gaatgaagat
    10861 ccagctgtat tcattgcaaa tgcattgaat cctgcacaag tcatggatgt gatcttcgat
    10921 gagacaaatc caaaggcctg cacagttgtc gttccagact accaattatc gctagcaatc
    10981 ggtaaacgcg gacaaaacgc acgtttagct gctaaattga ctaaccataa aatcgatatc
    11041 aaatcagaat ctgacatgac ggaattttat gaaaatcaag aacaacaaaa agaaacagaa
    11101 gaactgcatg atgaagcaat cgttcagtca gatttaacag acgatgaata tgaaacaatt
    11161 gcattcaaca atgaaacggt cgaagaaaaa ccagaagctt aactttagta aggaggcaaa
    11221 gagatgaaac aaaggaaaat tcctttacgc aaatccgtgg tttcaggtga aatgaaacct
    11281 aaaaaagaga tggtccgaat cacccgttca aaagaagggg aagtatcgat cgatccaact
    11341 ggaaaaatgc ctggacgagg agcgtatgtc tcacttgagc cggaagaagt acaacaagct
    11401 tgggataaaa aaattctcga tcgagtactc gaaacaacat taacagatga gttttatcaa
    11461 gaacttcttg actatgtcac tcaccaaaaa gcacgtaaag agttatttgg aaaatgaata
    11521 cagttaatcg gcaaaaagcc atgaatctca tcggtctagc gatgcgcgcg ggaaaactaa
    11581 tcacgggtga agaactgacc attgctgaaa tccgcaaaca aaaagcaaaa ttggtgtttg
    11641 tggcaaccga tgcaagcgag aatacaagaa aaaagataaa agataagagt tcatattatg
    11701 aagttccttg ctttgagcta ttttctgaag cagaaatcac gcagatgatc ggaaaacctc
    11761 gcaaggtatt cggaataatg gatgccggat ttgcgaagaa gaccaaggag ctaattgaag
    11821 gttaggaagg tgattgcatg ggcaacaaaa gaatctatga attagcaaaa gaactcaaaa
    11881 aatctagtaa agacgtcgtg gaaaaagcac aacaacttgg catcgatgtg aaaaaccata
    11941 tgggtgcgat tactagcaat gatgagaaga aattgcaaca agcgttcaaa aacccaagca
    12001 cgaaacaaca agctgcacaa caagcaagca gaaaaccagc caatcaacag ccgaacgatc
    12061 aacgtaaaaa agaaacaacg aacaatcaac aaaaaaataa aaataaccgc agttatcaag
    12121 atcgcggtca agggagcggt caagtgaatc aaggaaaaaa accaacaaca aaccaaaata
    12181 atacgggaaa taccagcagc caaaatcgtc aaggaacgag ccaaggcggc aatcaaaacc
    12241 gtactaacaa taacaacaaa ccaaacaata acaatcgcgg caaatttaat aataataacc
    12301 gtaatcgtta taataaaaaa ggtaaaaaag gcaaacagca acaatcaact aaaccagctg
    12361 taccaccacg caaattccgt gaattgccag atgtattaga gtatactgaa ggtatgaacg
    12421 tagcagatat tgcgaaaaaa attcatcgtg agccagcaga aatcattaaa aaattattta
    12481 tgcttggtgt catggtcaat caaaaccaag cactggataa agatacgatt gaactgttag
    12541 cgacggacta tggcatggaa ccacaagaaa aagtccaagt tgacattgct gatatcgata
    12601 aattcttcga ggcagaagaa gtcgatccag ataaattagt ctcacgtccg ccagtcgtaa
    12661 cgattatggg acacgtagac catgggaaaa caactttatt ggatacatta cgccattcac
    12721 gtgtaacaag cggcgaagcc ggcggaatca cacagcatat cggtgcctac cagatcgata
    12781 ttgatggtaa accaatcact ttcttggata caccaggaca tgcggccttt acaagtatgc
    12841 gtgcacgtgg agcaagtatc acagatatca cgatcttagt tgttgctgca gatgatggcg
    12901 tcatgccaca aacagttgaa gcaatcaacc atgccaaagc tgcaggcgtt ccaatcatcg
    12961 tcgctgtaaa taaaattgat aaaccaggtg caaaccctca acatgttatg caagaattaa
    13021 gtgagtatga gttaattcct gaagcatggg gtggagaaac gatctttgtt gagatctcag
    13081 cgaaattcgg ccaaaacatc gaagaattgc tggaaatgat cctgttagtc gcagaagtag
    13141 aagatttaaa agcagatcca acacaacgcg caatcggaac agtgatcgaa gcacgtcttg
    13201 ataaaggtaa aggtcctgtg tcaaccttac ttgtccaaga aggaacactt catgttggtg
    13261 acccaatcgt tgtcggtaat acttacggac gtgtccgtgt aatgacaaac gatcttggcc
    13321 gtcgcgataa agaagcagga cctgcaacgc cagtagaaat cactggcttg aatgatgtac
    13381 ctcaagcagg tgaccgtttc gtagtcttcg aagacgaaaa gacagcacgt gcagcgggtg
    13441 aagaacgcgc gaaacgtgca ttattagaaa accgtgctgc cacaagccgt gtgactttgg
    13501 ataacttatt tgaaagcctg aaagaaggcg aattgaaaga agttaacgta atcatcaaag
    13561 cagatgtaca aggttcagca gaagcactgg ctgcatcatt gaagaagatt gatgtggaag
    13621 gtgtccgtgt aaaaatcgtc cattcagctg tcggagcaat caatgaaagt gatgtaacct
    13681 tagcagcagc cagcaacgct attatcatcg gtttcaacgt tcgtcctaca ccgcaggcaa
    13741 aagtgcaagc tgatactgaa gaagtcgata ttcgtcttca ccgtatcatc tataaagcca
    13801 tcgaagaaat cgaaactgcg atgaaaggga tgctagaccc tgaatttgaa gaaaaaatca
    13861 ccggtcaaat gatcgttcgt gaaaccttca aagtttctaa agttggtacg attgcaggtg
    13921 cctatgtaac agaaggatat attcgtagag atagcggtgt ccgtgtaatc cgtgacggaa
    13981 tcgtaatcta tgaaggccaa cttgcaagtc tgaaacgatt caaagatgac gtgaaagaag
    14041 tcaaaatggg ttatgaatgt ggtgcaatga ttgaaaaatt caatgacatc aaagtagatg
    14101 acgtaattga aggatttatc atggaagaag tcaaaaatga ctaaaaactg ataaaaggag
    14161 gccaatcatt atggctaact accgtgaccg ccgagtcggt caagaaatct taaaagaagt
    14221 caatgatatt ttgcgcaaaa aggtccgtga ccctcgtgtg gagaacgtaa cgatcacaga
    14281 tgtgaaagtt acgggggact tacagcaagc aacgatctat tacagcattt tatctgactt
    14341 agcatcagat aagaaaacag cacaagatgg attgaataaa gcatcaggat tgattcgtcg
    14401 agagttaggg cacaatctaa gtatttataa gacacctgaa ttgttctttg aacttgatga
    14461 atcagttgtc tatggcaacc atatcgatga attgcttcgc gacttgaaca aagactaatc
    14521 aaaaactccc gcaaatcctg tgaaat
//
LOCUS       DACEKH010000069        14525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEKH010000069 DACEKH010000000
VERSION     DACEKH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.072"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(334..696)
                     /locus_tag="IW662_001942"
     CDS             complement(334..696)
                     /locus_tag="IW662_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866763.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="IW662_001943"
     CDS             complement(770..1150)
                     /locus_tag="IW662_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ0866764.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1232..2407
                     /locus_tag="IW662_001944"
     CDS             1232..2407
                     /locus_tag="IW662_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0866765.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2484..3068)
                     /locus_tag="IW662_001945"
     CDS             complement(2484..3068)
                     /locus_tag="IW662_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0866766.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIAKVQRGPQDRPVHDVVIETIEISE"
     gene            3269..4267
                     /locus_tag="IW662_001946"
     CDS             3269..4267
                     /locus_tag="IW662_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0866767.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4379..4888
                     /locus_tag="IW662_001947"
     CDS             4379..4888
                     /locus_tag="IW662_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ0866768.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4918..5577)
                     /locus_tag="IW662_001948"
     CDS             complement(4918..5577)
                     /locus_tag="IW662_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ0866769.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5579..6343)
                     /locus_tag="IW662_001949"
     CDS             complement(5579..6343)
                     /locus_tag="IW662_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ0866770.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6351..7028)
                     /locus_tag="IW662_001950"
     CDS             complement(6351..7028)
                     /locus_tag="IW662_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ0866771.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7065..8450)
                     /locus_tag="IW662_001951"
     CDS             complement(7065..8450)
                     /locus_tag="IW662_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ0866772.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8660..8744)
                     /gene="ffs"
                     /locus_tag="IW662_001952"
     ncRNA           complement(8660..8744)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IW662_001952"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="IW662_001953"
     CDS             complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="IW662_001953"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ0866773.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9389..10246
                     /locus_tag="IW662_001954"
     CDS             9389..10246
                     /locus_tag="IW662_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ0866774.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10327..10662)
                     /locus_tag="IW662_001955"
     CDS             complement(10327..10662)
                     /locus_tag="IW662_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866775.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10729..12099)
                     /locus_tag="IW662_001956"
     CDS             complement(10729..12099)
                     /locus_tag="IW662_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ0866776.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12177..12713)
                     /locus_tag="IW662_001957"
     CDS             complement(12177..12713)
                     /locus_tag="IW662_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0866777.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13022..14503
                     /locus_tag="IW662_001958"
     CDS             13022..14503
                     /locus_tag="IW662_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0866778.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4475 a         2892 c         2359 g         4799 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acttttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat tgtttcctcc taatatttgc atacttcata ataataataa cataatacct
     3121 aacctctcgt aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta
     3181 taacttaccg tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct
     3241 gctatagtgt gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc
     3301 aggtcctgta gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat
     3361 cattgacagt ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat
     3421 ttacgatatt cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa
     3481 acaattaact acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg
     3541 tgaagacgaa gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat
     3601 tttagcttta ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc
     3661 atttgaaaac cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa
     3721 agtcgctatt gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat
     3781 tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt
     3841 atcacgcttg aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc
     3901 aggcaataat ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat
     3961 tgatttgatg attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct
     4021 ttcttcttgg ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac
     4081 ttctattcca ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact
     4141 gattgctact gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa
     4201 tccaaaggaa cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc
     4261 tagttgattt tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt
     4321 tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat
     4381 ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt
     4441 aactgtagaa gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt
     4501 gactttggcg gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa
     4561 cgccattatc actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc
     4621 gttgcaacat attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt
     4681 gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt
     4741 gaaacctggt attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt
     4801 agatgatatc gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc
     4861 agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta
     4921 gacgagttct ttcttttttg tttgtttcaa gctatttttt cctaagaaaa gaaaaacagc
     4981 aaaaaataat tctaataata caaaaaataa gataaagcta tgcatgaaaa attcttctgg
     5041 aagtacattg atgattggat cagtaacggg atcgaataac cagtcatcgt tgttgaaaaa
     5101 cgtttcatga aaaaggataa aaaagcgatc aaatcctatc atcatgaaaa aaccgaaaac
     5161 aactggcagt aacatgccga tttgaaacgg acgaatcaac cgccaaagcc gatcattctt
     5221 ttgcaaatat ttgataaaca aaatactcgg gatcaaagtg atgaaaaaca cagcataatc
     5281 cactaaaaac aaaattttca cttcataaaa gtgatgggct ccgctagcag aaactgggaa
     5341 atctggcaag ctgaggatgg ttttgaaggg attattgaga taattcatta ggtgatcaaa
     5401 atttttcagt aacgtctctt gatctaaaga agtataatcc aaaatttgca aatgtccgat
     5461 atcaaatacg tatagtggtc gaaagttgat cgtcacacca actgccagac tgatcagtgt
     5521 cagaaatagg ctgaacaatc cagcatattc catccactgc catctcgaat ttttcatatt
     5581 agaagctcca ttcatccagt gattgtttca cataggtagc tgggataggc aacccaggta
     5641 catcttcttc ttttgtaaaa cctgataaaa cgagcaaagt atctatatta ttgcgtatcc
     5701 cagcttggat atctgtttca tagttatctc caaccatgat tacttcttct ttttgcagcc
     5761 ctaatacttc aacggcttta tccataatga tggcttctgg ttttccgatg tagatcggcg
     5821 gcgtttgggt tgctgtctct acaaaagaga tgactgaccc tgctccagga agaaggccgc
     5881 gttcagtagg tatattctta tctgggttcg ttccaatgaa agtagcacct ttttgaatag
     5941 caagtgttgc tttaactact ttttcatagg tcagataatt atctaagcct accactacat
     6001 agtcaggagc ctcttcttcc catgtaaatc cggcagctaa aatcaagtca atcaatcctg
     6061 cttctccgat aacatagact tttttccctt ttccatctgc tttcataaaa tcgattgttg
     6121 ccaaagtagc tgtgtagact gtctctggag ctacatgaat gtcaaactca tctgctagac
     6181 gattagctac tgtttctggt gacttagtcg tgttattggt aacaaacaag aaaggtaatt
     6241 ttcttttttg caattcttcc acaaatcgtt taccggccgg tatgggttgt gtcccacgat
     6301 aaatcgttcc gtctaaatca attaaatatc ctttatagtc cattcatcca ctactcttcc
     6361 ctttttctga tcgtaaatgc tgtttttgtt ttttctactt gtttaggttg ttcttgtttt
     6421 tttacttttt cagaggtttt tgctgactct gtttttcgat ttttaataac cggttttttg
     6481 cgattagatg cgactggtcc ttttttctca tcgatatacg cacgattatg gttttttttc
     6541 ttttttcggc gctgagttgt cttttctttt ttaccgccga ttctctcgat gacaaaatac
     6601 gcacaaccaa aattgcaata ttcgtaaaga tagtcttcca aggaatcgat tcgctgatct
     6661 ggatgcgccc gtcgattatc cgcttggaaa aatcctttta ggcgtagttg ttcataaccc
     6721 caatctccta cgatataatc atatcgggct agtacatcac tgtaacgttc tccaagcttt
     6781 tctgcattaa atccttcacg ataatcggac acaagccgat aatgtctttc acctatcaaa
     6841 aaattactgt catctaacag tgtcactagc tcgcctttga ctttttctgc tttaggctcc
     6901 tcaacgattt cttccaaagc agctgtcagt tcttcggtga ctgttgtttc ctgttcagct
     6961 gatgattttt tcttagcaat ttctgtttta tgctcaacgt tctgctgttc attttgtttt
     7021 gcagacatgc gttcaccacc tatctttttt attatacctt gtttttattt gattgggtag
     7081 tgagctttca agtaatctcc taccacactt cggataaatt cagggaacaa aaattcattt
     7141 tttccagcaa tctcaatcgt tggaaaaaat ggaacaaaca tcaaatgatc tacggtagta
     7201 aaagtataat ttttattcag atcgattggc ttgttcttcc aaaagacttg atggttaata
     7261 gaatcatatg tgattccatt atagaccatc tgcccaaaaa tcttaccgcg aaatcccatt
     7321 cccttcatag gatagttacg taaaaagtta cgattttttt ctatttctaa aactaagcgg
     7381 atcagatcat ttccacccaa tgtgacattt aacaagtgca tcggatgcgg caacgcagtg
     7441 tgaagctgat cctgattgac ccgccctttt ggaatttcag ttaaaaacag accactgttt
     7501 aatattgctg cttccgtctt tcctcgttct ttcaccgcct caaaggctac ttggataaac
     7561 gaatgctcat tgaagatatc tagactcaga gaaaatggca aatctgctac ttctttttct
     7621 tttaataatt catgcccacg ttctagataa ctatttattt cttgttcatc ttctgtaaaa
     7681 gctgtcattg tttcggtagg gatggctttg gctgaatgct taaggatttt tttctcttca
     7741 tcgatggtta aatggacttc accgacatac tgcccgtact ttccagcggc tgctatttgg
     7801 acatcattga ttacgtgacc ttctctgaat aaatgatggg tgtgcgatcc taaaataaca
     7861 tcaattgctg gtatttcttt tgcaatttgg aaatcatcct gaatcccgag atgactcatc
     7921 aaaactaaaa catccacttg tgaacgtaaa tcttcgacta attctggcaa aatatcataa
     7981 ggattccgta tgtcccagcc attcggatta taagtaagcg gaaatggagc agtaaatgca
     8041 attagtccga ttttcgtctg gtgtttcgtt gtaatgattt tatatggttt cgtccatgcc
     8101 ggtggctgga gcgtattttt atcgaataag ttatccagga tgatatcaaa ctcagcatga
     8161 tcgtataaat gattcaactc ttctttggaa tttcctacgc cttcattgtt tcctatcgtc
     8221 gctgcatcat aaccaacttc attcatcagc aagatatttt cctgtccatt cgttgcttct
     8281 gacaatggat gccaacgatc cacaaaatca cctagatcta ctgcaatcgt tgtcgttttt
     8341 tctttctttt cgttttctcg tttacgttga tccaaaaacc ttctgatcct tggccaattt
     8401 tctaaatgag aatgcagatc attcgtatgt aaaatcacta ttttttccat gtaccctctc
     8461 gcctctcttt atctttatca taccataggc agctttttct tccattgaaa caagctctat
     8521 ttcagagttt ttcctgcctt gaagcaaatt gtataagtac aatattcgat aaatcagtct
     8581 ggctaaaaaa tcaggaacat tcatttttac agtactataa aaataaaaaa aacagacccg
     8641 aaagtctgtt gtcgtttcat tataatcaga cacatggcgg aacctgctta gtgctgcttc
     8701 cttccagacc tgacacgctt cacaagccca ccattgtcct agcctttcgg caaagaatag
     8761 tataccttat ttttcattcg ttgaccagtc tttttttaat ttcttttttc gctggcggag
     8821 ctttcggaaa aagtcagtca aaatcatccc acattcgtct tctaaaatgc caccttctac
     8881 atatgcttgg tggttaaacc gctcgtcctc caaaagattc atcaaggtac cggctgttcc
     8941 gccttttgga tcatacgcgc cgaaataaac ttctgctaca cgagacaaaa tcatcgcgcc
     9001 gctgcacata ggacaaggct ctaaggtaac aaaaagtgca gcatcttcca agcgccagct
     9061 tcccactttt tcacaggctt ccttgattgc aagcatttct gcatgtgtag tagcatcttg
     9121 cgtggtttct ctaaggttat atcctcttcc aatcactttt ccatccaata caaccactgc
     9181 tccaatcgga acttcatgaa gcatttctgc tttctttgct tctgccaatg caaggcgcat
     9241 ccatttttct ttttctactg catcaagatt tgtatttacc aattaccctt ccctgccttt
     9301 gttttttctt gttttacttt agcatatgat aaaaaggctg taactaactt ttttttctat
     9361 ggtaaaataa atacaagagg tgaacaagat gaaaatggat aaattacttg ctctttaccc
     9421 acaagcacag aaaaaaaact ctccagcatc agaagcggat atcctttctt ttgcaattga
     9481 tgatgctttt atctggatca aacaagattc attatctcaa caagaaacca gccttttgaa
     9541 agcgttattc cctgtaatca acgatcaaaa aaaacaccca tggtatcgct atctttttaa
     9601 agatgcttct tgtgttcaag aaaaaagctt tcgtttgatc cagcttcacg tcgaatcaaa
     9661 aggagaattc ttaaaaaaag aatggcaaga tactattttg gaattatttc gctattctgc
     9721 tgatttcttt ttttatacag acacagatgc gctcttagtc gaacaaataa accgtgatta
     9781 tcttgatgct actgaaatca acggtatttt tctatcttta gatgctgatt ttgatacgac
     9841 aacgtctgta ttcgttggtt ctttccattc acctggcacc gacctcgtcc ctttgtttgc
     9901 tgaagaaaga aaaatttttt taacagagca aaacaatctt tatactcaca gccgtacttt
     9961 ttctatgcaa gatgtggctt tacattacta tacaaaagat actgtaaaag atagtaagtt
    10021 gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg gaagtgatca ttcaagaatt
    10081 atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca ctgtttcttc atcgtaatac
    10141 tctaaaatat cgaatcgaaa aatttcaaga acagtctggt ttcaatctaa aaaaagccaa
    10201 cgatttactg ttttgctatc ttttattgct tcatgaacat aattaaagat agattaaagg
    10261 ggttgtgaca ataattttgt cacaacccct tatttcgaat gaatagtgtt caaaagctag
    10321 tttctcttac atcatttttt caacaaaatg cattagccgg tcttttacat cttcggttga
    10381 atctttgatt gaatcaccat aaacagagat tttctttttt cctgatttga tttttgctaa
    10441 cattcccatc aaagaatcca agtcactatc tgaaagacca tctacgattt ctagtaattt
    10501 ttcatttcca tcaaatttgt catagacgaa tttttttact ttcgtccgag tagtcatatg
    10561 atggatcttt tccattactt ttcccgatac aacgactgct gtcgctacac tggcagcggc
    10621 tgctacgctt aatccgattg ttacttttgt tgatgttttc ataagtatca gctccttttt
    10681 atctatctcc atcatagcat agacaaaaaa ataaacgaac taatccactc agtgaaatct
    10741 caatccttta gggaaaaagt tctttactgt tccttttact tgtttcccaa atccgacagg
    10801 tatcttatct aatactagca aaacccatcc ttgattccca tcacgttgaa atgtttctcc
    10861 tgctgtgtaa gattgccatt ccttttgagt gatcggcaag caaggtatat tctctatctt
    10921 ttttgtagca agcgctaatg catagctagg ttcaaatcga ttctttttga aatctcctaa
    10981 atgcaaacct gtacgtacga cttttaaacc atcgagagaa ggggctaact caggaacttc
    11041 ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct tcataatgaa aatcatttga
    11101 aaactccgtc cagagttttt cctgctcttt tgtcatttgc actttacttt tttttcgtgt
    11161 cttcttttct ttatgcatct gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc
    11221 ttcaccctga tctttatgag gccatatacg tattgttttt tccaaaccag ctacgcttcc
    11281 ccattcagaa cgtcctgaac taacggattg agtcaaagga atctcctcaa tcgttactgg
    11341 ataattttct acaagccagc taatgatctc ttcattttct tctggtgcaa acgtacaagt
    11401 cgaataaatc agttgtcctt tattttttag catttttatt gctgaagaca aaatctcttg
    11461 ctgacgtttt tgacagtata gcggagattc ttctgtccac tcttttattg catttggatc
    11521 ttttcggaac attccttctc ctgaacaagg agcatcaacc acgatccgat caaaaaagcc
    11581 cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc
    11641 ccatcgttct atattttctg ataaaatctt cgcacgtttc ggaaagatct cattcgtcac
    11701 taataaccct ttccccttca tttgagcagc taattgggtt gatttaccac cgggagccgc
    11761 acacaaatcc aaaacttttt caccgggttt tgctgcagca gctgttccaa cgatcattgc
    11821 acttggttct tggctatatt catacccagc ttgatggagg aaagactttc cattgacagt
    11881 gccaagaaat ccctcattgc tgtagggagc gggttgtaat tcttcgctat ggtaagtttg
    11941 gaccatgtcc aatcctgccg gttttagtgg attccaccga aatccttttt tcacgcttcc
    12001 ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct aaaagaagac gatatttttt
    12061 gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt
    12121 ttacaatctt ttttacgaaa aataaaggtg gctgatctac tttttcatgt tgctgcctaa
    12181 ggtgactgtt cctttaagat attttccaag tattcatccg tcacctcgtt tttatcccaa
    12241 acagcgttca tcatttcaag aaaatcttga gaagaatcat ctgagcgcaa acttcccaat
    12301 gcattaagcc ataccaattc aagcgacttt tcttctttga tcttcgtgtg acctttgaca
    12361 tatgagaaaa atggccgctt aaatagcgaa tccatgtcta aatcagattt tgcaatgatt
    12421 tcacgaatac tttcatggat agaaaaaaaa tctttcaata agtagacaat gcttaaaaca
    12481 tcatcggaac tgcattcagc taagtagttt tcaaaataaa cagtgatttc ttctaatatc
    12541 ttctctttta acccgctgat tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg
    12601 cagaactctg ctaactctct tgcagtaatt gacttcattc catattcttc cacgtatacc
    12661 atacttttga acagcaaata ttctttacta taatttgtta ctctccccat cattcttacc
    12721 tccgtctctt ttcatctacc cataaataaa taacataaat aattctaaaa atattattga
    12781 taattatcaa aacatattta gttatatcca ttaaaaatca atttttccgc cttaaaaaca
    12841 agaatatctt gacaaaatac cctaggaaat aaacaataga ataaacaagg taacaaaaaa
    12901 attaaataac gttatataaa aaagaaggcg acactctgtg agaataacac agactgaaat
    12961 gactttccac tcacagtttt tgtctaacta tatacaaaaa cagaataaaa aggaggtatt
    13021 tatgctaatt gaagcaagaa aaaaagagtt acttgttcta caaatggtca ttcaaaaaaa
    13081 taattgttct ttacaagaat tatccaatga cttacatatt ccaaaacgga caatcaaaga
    13141 actgatccga aaaataaatg tatcactcaa gcaattttta ttgattgatg aatttattta
    13201 ttctaatcat aaaggagaga tccaaaccaa agaaaccgat caaactaaaa agttagctgt
    13261 tttttctaag ttaaaattat gctacctaaa agaatccaat cgttttaact atttgatgtt
    13321 actaatcaat tatccacaaa ctgctgtacc aaaaaaatat ttacttgaac aactttatat
    13381 atcgccctcc tatctcgaaa aactgacaaa acagctcaat cattccttaa aaaaattcca
    13441 tttacggatc ctatctgtaa aaaattgctg tactcttgaa ggaaatgagt tatctatccg
    13501 tctctatctt tacttttttc tttcagatac atttggaggt aacgattggc cattttcctc
    13561 acttgaatta aatgcactaa aaatgaacag tacagatgag caaatagaaa aaatttcaaa
    13621 acaagaaaaa aatatctact tgctgttaac tttattcata ttacgaaaca aacaacaggc
    13681 atattttcca aaaacttctt ctgaaatcac tgaattgatg gacttattgc agcatacaca
    13741 tgattattca gaatacttta cttctttttc agtgagtcgc ccttgtgaag agacagaaac
    13801 agagagagcc tactttaatt attttttcta ttgttttttt ccaaatcaaa tctcttttcg
    13861 acaaaaacag gagattggtt ttttattttc cacaagcaaa aaccttattt gtcgacaagt
    13921 cggtcaattg atgacttact taactcaaca gtttcctctc aaccatttag aaaataaaat
    13981 accaatttat ttttattttc ttgtgttggc agttgttttt ttcgaactgt tgggtgaatc
    14041 cagcagtagt ttttctacac tttgctttcc agaagccacg tccaatcatt ctagttcttt
    14101 tatacaggaa aagaaattaa gagaaaaaat cgatacaatt ttttcaaaaa atggggaaag
    14161 aaaccaaatc agtcaatacc ttagtcaatt gatccaacac ttactacaaa cagaaacgaa
    14221 agagcagtta ctcatccatt tgcagataac caaacagttt acaggaaatt atcatcttca
    14281 aagccatttg cagcagttat ttcgcaaaga tgctgttaaa attaccgcat attcagaaca
    14341 tgctgatttg atcattacag atcagcatga gaaaatttca gatgaaacgg agactttcta
    14401 tctggataca acgcacaata cagtgttatt tggcaagtta ataaaagcgg ttcatgaact
    14461 gtatatgaaa aaaggttatg agaacaaaac aaagaggatg tgacaaaatt cttgtcacat
    14521 cctct
//
LOCUS       DACEKH010000070        14332 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEKH010000070 DACEKH010000000
VERSION     DACEKH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.073"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(244..813)
                     /locus_tag="IW662_001959"
     CDS             complement(244..813)
                     /locus_tag="IW662_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0866779.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(887..2176)
                     /locus_tag="IW662_001960"
     CDS             complement(887..2176)
                     /locus_tag="IW662_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866780.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2530..3378
                     /locus_tag="IW662_001961"
     CDS             2530..3378
                     /locus_tag="IW662_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ0866781.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3392..4465
                     /locus_tag="IW662_001962"
     CDS             3392..4465
                     /locus_tag="IW662_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ0866782.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4455..6626
                     /locus_tag="IW662_001963"
     CDS             4455..6626
                     /locus_tag="IW662_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAQ0866783.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6738..8909)
                     /gene="copB"
                     /locus_tag="IW662_001964"
     CDS             complement(6738..8909)
                     /gene="copB"
                     /locus_tag="IW662_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ0866784.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(8929..11115)
                     /locus_tag="IW662_001965"
     CDS             complement(8929..11115)
                     /locus_tag="IW662_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0866785.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11115..11324)
                     /gene="copZ"
                     /locus_tag="IW662_001966"
     CDS             complement(11115..11324)
                     /gene="copZ"
                     /locus_tag="IW662_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ0866786.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11337..11777)
                     /locus_tag="IW662_001967"
     CDS             complement(11337..11777)
                     /locus_tag="IW662_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ0866787.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11851..12324)
                     /locus_tag="IW662_001968"
     CDS             complement(11851..12324)
                     /locus_tag="IW662_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HAQ0866788.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12634..14100)
                     /locus_tag="IW662_001969"
     CDS             complement(12634..14100)
                     /locus_tag="IW662_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0866789.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4095 a         2991 c         2524 g         4722 t
ORIGIN      
        1 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
       61 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
      121 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
      181 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
      241 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
      301 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
      361 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
      421 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
      481 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
      541 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
      601 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
      661 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
      721 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
      781 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
      841 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
      901 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
      961 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcggtgatc actagatctg
     1021 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1081 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     1141 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     1201 gtaaaagcat cccccactct actgcggggt gtgcaaatgc ttgttggatc gctagccgaa
     1261 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     1321 cttcttctat cagccggtca tggataccaa ttacgtaacg aagccattct tggtaaagta
     1381 cttttgtcag ctttgatttg ttttcgctct caaattcgct attcagccaa aacagcgaat
     1441 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     1501 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     1561 ttattggcaa aatctgctct tctctaaata gcaattcagg aaacaattca aagaatagtg
     1621 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     1681 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     1741 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     1801 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     1861 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     1921 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     1981 gaacggtttt cttatgaaga atcagctgga tgggacagtc gctttccgcc aaacgtttca
     2041 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     2101 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     2161 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     2221 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatttt
     2281 cctcattatt tttaatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     2341 taaaaaaaca taataaaatc actttgcttg taggatgtat tcccttccat tacaatgaaa
     2401 taaatagcaa ttttatgaga aggtgatgtg aaaatggaag aaaaaatcga agaatccaaa
     2461 caaaaaatcg atcagttgat acaagaatta gaaaaagcgg tcatcggcaa acgttcaact
     2521 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     2581 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     2641 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     2701 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttggca
     2761 gatgagatca atcgcacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     2821 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     2881 acgcaaaatc caatcgagta tgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     2941 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctactttta
     3001 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3061 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     3121 cagttagtgt cagcaacaag aagccatgct gccatccagc ttggcatcag tccacgagga
     3181 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     3241 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     3301 gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc atactcttgt tgcacaaatt
     3361 cctgttcctg tgaggtagat tgaggtagat aatggaaaaa atcaaagatt atttcattaa
     3421 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     3481 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     3541 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     3601 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     3661 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aaacaagaga ttttgattct
     3721 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
     3781 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
     3841 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
     3901 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     3961 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4021 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4081 tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa cctctgctag acttgtattt
     4141 ttctttatgg catttgagaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     4201 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     4261 agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     4321 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     4381 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     4441 aggaggtgaa tggattgacg attaaatggg aaaaatggct gtgtggcgtt tactcctttg
     4501 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     4561 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     4621 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttgtat tttccttcga
     4681 agacattttc cttattatgg atcttacagt ttttttcgtc agtcacagag gaattcttcc
     4741 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     4801 tgctgatcta tttatttatt acattgaccg tggattacga ctatttgtac gcaccctttc
     4861 tttctttgat ggcttacttc atgatgctta ccgtttttcg ggaaaaagat ttactgtacg
     4921 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     4981 tgcggttgtt gtcgggaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5041 tcttaactat tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     5101 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgat acactccatg
     5161 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     5221 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaaaaagga aatggctatt
     5281 ggaagatcga aaataaaaat cattacaccg gttctggatg gatagaagat caggacgcac
     5341 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatggaag
     5401 acaaagaaaa cattacattg accctaaaaa aacaagcttt cttaccttat ccttatggaa
     5461 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     5521 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     5581 ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt aaaagctgat gatttagaaa
     5641 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     5701 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc gaacagtatt
     5761 taaaaacaag cggaaatttc cgctattcca aaacggatac cggacatact ccgaaagaac
     5821 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     5881 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     5941 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6001 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6061 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     6121 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     6181 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     6241 tattcagcct tggggtgtgt ttatacgtct tgttgttctt tctattgtta ttcagaaaat
     6301 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     6361 catatcaaaa acttcttaac catttggaaa cattggtacc gcgcaaaccc gcagaatctt
     6421 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     6481 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aatttcgata
     6541 aaaaaaccta tttagaaatt acgcatataa taagaaaaac ggtatacaaa aaagttctga
     6601 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctggaatgc
     6661 cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag gttgtgacac gttttttgtc
     6721 acaacctttt ttcattatta tttgattttc aatgtcaaag catttagagc tacaactacc
     6781 gtacttaacg acatcaagac agcaccaact gctggactta atatgatacc aattggcgca
     6841 agaattccgg ctgctagcgg aattgcaaga atattgtaac ctgcccccca ccagaggttc
     6901 tggatcattt tctttcgcgt ttgcttggct agatcaagaa aatgcaaaat atcttttgga
     6961 tcgctgtttg tcaatacgac atccgcagaa tcgattgcga tatctgtccc tgcaccgatt
     7021 gccatcccga ttgaagcacg ggctaaactt ggcgcatcat tgatcccatc acccaccatg
     7081 attacttttt tcccttggtc aaggtatttc tggacaacag cttctttatc atctggtaat
     7141 aaaccgccat aatattcttc gatccctaaa taatctgcta ctgcttgggc agcgttttta
     7201 ttgtcaccag taagcatgac aggagtgatt tttctttctt tcaatgttcg gataaattct
     7261 tttgcttctg tttttactac gtcacctaaa gcaacgattg cagctagatg attttctaaa
     7321 atcaggaagc tgatggtatt tccttgttct tggtaaggtt tcaataatgc ttgttctacg
     7381 tttaatccga gacgctcaac ttctttttca ttaactattt tcacttgttg gtttttgact
     7441 gttgcctcta gcccaactcc tgaaagattt tttagatttt cggcttgata aggcttgatt
     7501 tcatgttctt tcaaataatt cataatacca acagccaatg gatgattggc attcgcttcc
     7561 aatgctccta aatattgaag gatttcattt ttattgaacg catcatcaag tacttcgaca
     7621 cccgttacag taaattttcc ttctgttagt gttcctgttt tgtccagcat gatcacatct
     7681 agatcattgg cttgctccag tgcattgcgg tttttcaaaa gtaatccgtt tttagctgct
     7741 attgatgtag agcgagcaac aactaatggg atagctaaac ctagagcatg aggacaggcg
     7801 atgataaaga ctgttaccat acgttctaat gcattaggaa gatctgtcaa gaacaaccag
     7861 gcgatgaatg ccaggatacc cacaattaat gcgacataaa acagccattt tgccactttg
     7921 tcagataatg actctagctt ggatttttct ccttgtgctt ttcgcaccat ttccataacc
     7981 ttcgctagat aaccattttc accagttcca gttacttcga tttcgatcgt tccatcccca
     8041 ttgatagaac cgccaatcac tttgtcattg gcttgttttt tgattcctct tgattcgcca
     8101 gtcacagctg attcgtcaac aattgtttca cctttcaaga ttacaccatc tgttggcatt
     8161 ttatcaccag accggacgat caaacgatca ccttcgttta cttctttcaa tgaaactgtc
     8221 tcttcttttc catctttcgt caagcgtttt actgattctg gcagtaattc tgctagtttt
     8281 tgtaaagcat tgctggcatt cgacacagca ttcatttcaa tccagtgacc tagtagcata
     8341 ataacgatca aagtagccaa ttcccagaag aaatccatga cgtgtgtatg tggattgagc
     8401 agatttgcga taaacgaata gacactataa atatatgcaa ctgtaatccc catcgcgatc
     8461 agtgtcatca tagcaggact tttttgtttc aattccatct tagctccact taaaaatggc
     8521 tgaccgccat agataaatag tatagttgct aagatcaata cgagccaatc agaaccagga
     8581 aacgttactt gaaatggaaa ttccattccc atcattggtg aaaaaagaat gattgggata
     8641 gctaggataa gcgatagcca aaatttttgt ttgaaatttc ccatgtgcat ggaatgatcc
     8701 atgccgccat gatcgtgatc catgtgcatc gaatggtcca tttgtttgct gtttgctata
     8761 tctccatggt tatgtcccat gtgcatggaa tggtccatct ggctatggtc atgtttcatc
     8821 tccatctgtt tttcatgaga ttctgtactt tctttgttta ctacttcttt tttatgttca
     8881 tgttttttct ccatatcgtt cccattcatt tttactccat cccttctctt actttttcat
     8941 gtgccgattt aagctcaggg agttcaacaa tacactgacg gaactaaacg ccatcgctcc
     9001 tcctgcaatg attggattca aaaagcctag tgcggcaaaa ggaataccaa tcgtattata
     9061 gacaaaagcc cagaaaagat tttgtttgat ttttctcaat gtcaccctag acaactcgat
     9121 tgtttgtgca atggatgcca aactgctact catcagcgtc acatcggctg tttccatcgc
     9181 gatatccgtt ccacttccca ttgccatacc aacatcagct aatgctaaag ccggtgcatc
     9241 attgattcca tcgcctgcca taccgacttt tttgccatct ttctgaagtt tttctacgta
     9301 ggctgcttta tcctctggaa gtacttcagc aaagatatgc tttggatcaa taccaacttg
     9361 tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc agcataaaaa catctaatcc
     9421 tttattctgg agctgcttga ttgcttgttt ggcttctagt ttgatttgat cagccacggc
     9481 aatcaacccg atcacttgct tttcatcagc taaaaacatg actgtttttc cctgctgttc
     9541 taattctaac gcgtgttctt ggtattcatc aaatgatagg ttcaattcaa ttaatcgttt
     9601 acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg ctgattccag cacctgggtg
     9661 agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc agttttccgt attccacgat
     9721 tgctttacct agtggatgtt cagaagcttg ttctaaagta taaaaaatag ataggacttc
     9781 tttagaaccg actagatctg taactttggg ctttccttct gtaatcgtcc cagttttatc
     9841 caaaacaatg ctgtctaagt gagcagctgc ttctaatgct tctccgcctt taattaaaat
     9901 tccatttcgt gcaccaacac ccgttcctac cataatagct gttggagtcg ctaaacctaa
     9961 tgcacatgga cacgcaatga ctaatacaga gacactatgg atcaatgcga gttgccaatc
    10021 accagtgatc aaacctgtag ctatgagcgt gataaacgcg atgactaata cgatcggaac
    10081 aaaaatccct gagatccgat ctgcgatttt ttggattggg gctttgctcc cttgagcatc
    10141 ttccaccatt tgaatgattt gcgctaatac tgtctgcttg ccgacttgtg tgacttccat
    10201 gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc gtgtcatccg gattcttttc
    10261 tacaggaaga ttttcaccag taagcatact ttcatctatc gcactgcttc ctgaaataat
    10321 tcgaccatct gctggaatct gctcaccagg acggatgact agttgatcac caacgactac
    10381 ttcttcaatc gctaacgttt cttctttccc gtttcttatc acttgggcgg tttttgtttg
    10441 aagagacatc aattgtttga ttgcattacc tgttttcgtt tttgctgtat gttccaaata
    10501 tttgccgagc aaaatcagcg tgatgatcat actgctgctt tcaaaataaa gatcttgggg
    10561 gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag gcagccgatg ttccaattgc
    10621 taccaataca tccatatttg gtgctttcga cttgattgca tggtacgcac ctttataaaa
    10681 acgagcaccg acataaaatt ggacaggagc agacagcact aactggacga ttggaaaatg
    10741 aaaaaagtga acaatcgccg catggcttcc taacatcata gcaatcattg acaacatcaa
    10801 cggcaaagtc aaaaccgtac tcaatataag atcgaatagc atttttttca aatacgcttg
    10861 cttttcttct gcaatttttt gtttgtgggc ttcatcgtac aaaattgcac catagccgat
    10921 gttttctacg ctttggatta atttttcagt ggtcgtccct tcatatttga cacttgcttt
    10981 ttcagtggcc aagttgactg tggcattgac cacaccaggt tgtccgttca gctctttttc
    11041 gatcctagca gaacaatttg cacaagtcat acctgtaatg acgaacgtat ccattttggt
    11101 ttctgctgtt gccatcaaat cacctctgct ttataaccca atccattgat tgcttgacaa
    11161 atcttttcgg attgaacttt tgtttcatca aacttgaccg taccgtttgc ttttttcaaa
    11221 tttactttca ctttttgcac gccgtcaagt gcgcttactg tttcttctac tcttgctgca
    11281 caatgattgc agctcatacc ttcgattgaa aattgttgtt tcattttttc ttcctcctat
    11341 tgatgttgtt tacattcaca ttgacctggg atacagttac attcgatcgt atctactggt
    11401 tgcttttgtt ccagttgttt caaaatctca ttgatatctt cttgagtcaa agtggcttct
    11461 tctactagat ccgcaatcgt ctctccaatc ctttttgcac agatatggga aaaaagattt
    11521 tccgtcgcac tgcgaactgt ttccatctcc gagacagctg gatgataaat gaattttttt
    11581 ccttcttgtt ctgtccagat cacttctttt tttaccaaac gacccagcaa agttttgatc
    11641 gttgctggtt tccaatccat cgaatcagct aagatctgcg tgatttcttg ggctgtcgtc
    11701 tgtccaagtg tccaaataac acgcatgatc tcccactctg aatcactaat ctttactggc
    11761 gttttgactt tttccattta tttcacacct ttcgtttaca aatgtaatca ttaacttatg
    11821 aatacagatt acacttgtaa acaaaaactg tcaattattt ttgctctcta ttttcaattt
    11881 agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg atcagtttat ttcttgcatt
    11941 agctacttgg atcagctgct gtatttttgg ttcattgttt ccttcaaaat gccagataaa
    12001 agataaaaat tctaagtatt tcctgtttag tttctccgaa aatgattcag gcatatctgg
    12061 ccgagatttt ttgtttatct ttctttttag agaacgccaa actactcggt agatggcgag
    12121 ataacgcggc actttaagcc aaatgatctt atctgcttct ttaatccttt cctctaacgt
    12181 agaagtataa tttccttcaa cgatccatga cggattagat accataaacg attgctgttc
    12241 ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt gatttctttg atcttccttg
    12301 cgagggacga ttttcccgac ccactttgtc caatcacgat gatcttcatt cttctccctc
    12361 acttctcttt ggaatttggt tcgatcatac cacttataac gccgcaaaaa aacaagggac
    12421 tgtgacaaaa gtcgtgtcaa agtcccttgt ttcgaataaa tggtgttcaa tcagctgacc
    12481 ttcagcatta gctcaaaaaa cggcaaaata tgaggagcta tttttgtttg gctgttctta
    12541 ttacttgatg caggacagct ttttcacaac ctctgattca cggtgttcca gaagtagctt
    12601 ctgtcacaac ctcttgtaac aagtatttct attttaaggc tgaaccactg tttctggttc
    12661 agtaatttct ttgtttttgt ctcgacgagc aattgctggc aagatcatac ctaataagat
    12721 caagacaatt ggtgtgatga tattcgtgaa taattggaac caccatgctt taggatcagc
    12781 agtataatct acttttggca ccattcctag gatacatgca aacgcagtga aggcaaagca
    12841 ccaagcacca aagataaaac cgatttttgg atttttaaca aatttatatt cagaattgaa
    12901 tttcttataa gctttattca acatcatata tgccaagaac acccataggt aacgcattgg
    12961 catgacaact gagttcaagt tcgtcaacca ttttactaat tcattcatat ctttgatacc
    13021 aaagatcggc aacaaaataa tgattcctac tagtatacct gtcagaagat aaccattcac
    13081 caatgtacct ttacgattac gtttacgtag ccagtttggt acaaattctg gatccgcatc
    13141 tgctaacaag atttttagtg gtgcatcaat tgaaaaggct aatgcggaga tttgtccaat
    13201 catgtttgtc aatgcgtaaa tgatcatcaa tgagttaccg ataccatagt aattacctaa
    13261 aatttgaaaa gcactgtatg ccccatttgc catcaagtct ttagggatat tatcgctagc
    13321 aaacagcata cccattgcga ctgagcctaa tacagcacaa atccctacca ttgctgctaa
    13381 gaagatcatc cctttaggaa attcctttgc aggatttctt gtagagttga cataagggga
    13441 tattttttcc gcccctccaa ctgcaaatac tagcatcgaa acggttgtga aataactgaa
    13501 atcaaatgtt ggaatatatg ttttcacttg attcatatgt ggtgtggcaa attccatcgt
    13561 tggattgatg aacggtgccg ttacagctaa aagaataaat aagatcgaca tgacgaacat
    13621 cgctgttccg gccagtccgc caatcacttt caaagtagaa agtccttttg ttgataaata
    13681 taaaaaggca agaaatataa taagtgaaat aactgctacc atttgtactg acatcgtatt
    13741 tacgatattt ccatttcctt ggactgccca accaccagca atcagaattg cttgaggctt
    13801 ttgtgccaaa taaggaatat ggacgaccca gtaagtccaa gcggcatagt atgccagtcg
    13861 tttattagat gtcgtatttt cgacccatga actgacgcct cctttactat ccttgaaggc
    13921 tgatcctaat tgtccaacga tcaatgcgta tggaataaaa taaatggcca aaatcaatag
    13981 ccaagaggtg acaactgata ctccttgctg agcatagtta ttaactacat tccccatgcc
    14041 ccaaaccatg ttaaatgcaa ttaacgcaac agtaaaccag cgtaattgtt tgttgttcat
    14101 tcgttctctc ctttatgata aaaatttgtt atccagatgt aatcatattc aatttttaac
    14161 aaacttacaa gacagtgtta ttttatgtgt gtattctttt atttaataca catcgttttt
    14221 tcatattttt tccatcaaaa aaataagtta ttttctttta tttcattgac cttattgaat
    14281 ttcaagctat tttgaatttt ctgcaaaatt aaaaacatta gttattttat aa
//
LOCUS       DACEKH010000071        14144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEKH010000071 DACEKH010000000
VERSION     DACEKH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.074"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="IW662_001970"
     CDS             complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="IW662_001970"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ0866790.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="IW662_001971"
     CDS             complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="IW662_001971"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0866791.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8128..9096
                     /locus_tag="IW662_001972"
     CDS             8128..9096
                     /locus_tag="IW662_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0866792.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9114..10019
                     /locus_tag="IW662_001973"
     CDS             9114..10019
                     /locus_tag="IW662_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0866793.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10016..10822
                     /locus_tag="IW662_001974"
     CDS             10016..10822
                     /locus_tag="IW662_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0866794.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10979..11947
                     /locus_tag="IW662_001975"
     CDS             10979..11947
                     /locus_tag="IW662_001975"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ0866795.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12264..12788)
                     /locus_tag="IW662_001976"
     CDS             complement(12264..12788)
                     /locus_tag="IW662_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ0866796.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13075..14144
                     /gene="scm"
                     /locus_tag="IW662_001977"
                     /pseudo
     CDS             13075..14144
                     /gene="scm"
                     /locus_tag="IW662_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
BASE COUNT         4006 a         3050 c         2793 g         4295 t
ORIGIN      
        1 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
       61 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
      121 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
      181 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
      241 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
      301 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
      361 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
      421 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
      481 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
      541 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
      601 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
      661 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
      721 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
      781 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
      841 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
      901 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
      961 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
     1021 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
     1081 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
     1141 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
     1201 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
     1261 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
     1321 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
     1381 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
     1441 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
     1501 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
     1561 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
     1621 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
     1681 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
     1741 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
     1801 tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
     1861 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
     1921 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
     1981 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
     2041 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
     2101 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
     2161 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
     2221 atatcttgag aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
     2281 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
     2341 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
     2401 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
     2461 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
     2521 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
     2581 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
     2641 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
     2701 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
     2761 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
     2821 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
     2881 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
     2941 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
     3001 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
     3061 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
     3121 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
     3181 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
     3241 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
     3301 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
     3361 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
     3421 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
     3481 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
     3541 gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag
     3601 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
     3661 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
     3721 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
     3781 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
     3841 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
     3901 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
     3961 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
     4021 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
     4081 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
     4141 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
     4201 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
     4261 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
     4321 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
     4381 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
     4441 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
     4501 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
     4561 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
     4621 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
     4681 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
     4741 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
     4801 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
     4861 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
     4921 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
     4981 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
     5041 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
     5101 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
     5161 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
     5221 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
     5281 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
     5341 tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
     5401 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
     5461 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
     5521 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
     5581 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
     5641 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
     5701 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
     5761 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
     5821 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
     5881 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt
     5941 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
     6001 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
     6061 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
     6121 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
     6181 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
     6241 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
     6301 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
     6361 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
     6421 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
     6481 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
     6541 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
     6601 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
     6661 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
     6721 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
     6781 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
     6841 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
     6901 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
     6961 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
     7021 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
     7081 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
     7141 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
     7201 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
     7261 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
     7321 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
     7381 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
     7441 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
     7501 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
     7561 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
     7621 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
     7681 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
     7741 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
     7801 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
     7861 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
     7921 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
     7981 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
     8041 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
     8101 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
     8161 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
     8221 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
     8281 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
     8341 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
     8401 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
     8461 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
     8521 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
     8581 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
     8641 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
     8701 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
     8761 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
     8821 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
     8881 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
     8941 ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
     9001 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
     9061 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
     9121 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
     9181 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
     9241 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
     9301 cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca
     9361 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
     9421 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
     9481 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
     9541 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
     9601 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
     9661 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg
     9721 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
     9781 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
     9841 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
     9901 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
     9961 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    10021 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    10081 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    10141 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    10201 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    10261 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    10321 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    10381 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    10441 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    10501 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    10561 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    10621 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    10681 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    10741 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    10801 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    10861 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    10921 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    10981 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    11041 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    11101 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    11161 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    11221 acaaacgatg ttgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg
    11281 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    11341 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    11401 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    11461 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    11521 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    11581 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    11641 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    11701 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    11761 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    11821 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    11881 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    11941 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    12001 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    12061 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    12121 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    12181 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    12241 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    12301 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    12361 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    12421 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    12481 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    12541 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    12601 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    12661 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    12721 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    12781 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    12841 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    12901 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    12961 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    13021 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    13081 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    13141 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    13201 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    13261 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    13321 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    13381 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    13441 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    13501 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    13561 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    13621 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    13681 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    13741 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    13801 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    13861 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    13921 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    13981 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    14041 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    14101 acagtacaga tacatctacc acaagtgata gctcagattc atca
//
LOCUS       DACEKH010000072        14046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEKH010000072 DACEKH010000000
VERSION     DACEKH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.075"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            304..2469
                     /gene="acm"
                     /locus_tag="IW662_001978"
     CDS             304..2469
                     /gene="acm"
                     /locus_tag="IW662_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ0866797.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2741..3226)
                     /locus_tag="IW662_001979"
     CDS             complement(2741..3226)
                     /locus_tag="IW662_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ0866798.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3706..4347
                     /locus_tag="IW662_001980"
     CDS             3706..4347
                     /locus_tag="IW662_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0866799.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4366..5001
                     /locus_tag="IW662_001981"
     CDS             4366..5001
                     /locus_tag="IW662_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866800.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            5019..5834
                     /locus_tag="IW662_001982"
     CDS             5019..5834
                     /locus_tag="IW662_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0866801.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6153..7589
                     /locus_tag="IW662_001983"
     CDS             6153..7589
                     /locus_tag="IW662_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0866802.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7778..10225
                     /locus_tag="IW662_001984"
     CDS             7778..10225
                     /locus_tag="IW662_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0866803.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10455..11522
                     /gene="rlmN"
                     /locus_tag="IW662_001985"
     CDS             10455..11522
                     /gene="rlmN"
                     /locus_tag="IW662_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0866804.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11715..12818)
                     /locus_tag="IW662_001986"
     CDS             complement(11715..12818)
                     /locus_tag="IW662_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0866805.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13371..13919
                     /gene="aac(6')"
                     /locus_tag="IW662_001987"
     CDS             13371..13919
                     /gene="aac(6')"
                     /locus_tag="IW662_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0866806.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4830 a         2311 c         2961 g         3944 t
ORIGIN      
        1 ttttatgaaa ctatcgttat ttattaatgt tgcatttttg taagttaaaa attggaaata
       61 tagattgaaa aaatgaaagc aataagtaaa ataaaatgaa tatatgcgaa aaaataattt
      121 acattaggaa aaaccttgta aaaacagttt tttggcatat ttatttttta taaatatttg
      181 taaaataact atcaattttt tagttaaaag attggaaatg agtgggggag gaattgaaga
      241 aaggattttt gagagatgat atagtaggtt ttatttttag ttaaaatagg ggagatagaa
      301 aaaatgaaaa agtgtagaaa tattatttta tcaatgttat ttattattac taatataaca
      361 tcactgattc ctgttcatgt ctatgcggat gcaggcagag atatcagcag taatgtcact
      421 tcgttgaccg tggacccaac gaatattaca gatggaggaa acataaaagt aaaattctcg
      481 tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc tttggatcaa ttggccaagt
      541 gaaggaaata ttcgcggtga aggattccaa aaagaaatcc cattaatgat tgaaaataaa
      601 aatgttggaa ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa tgaaaatatt
      661 aaaaacctag actcagtaga aggttggggg caatttgaga tacaggccag aaacgtaacc
      721 gataccggtg aagaaaatac gggaccattc accgtgacga gcggtgataa aacagctact
      781 gtaaatgtga caaagccagc ttctggttct tcttctagcg ttttttatta taaaacagga
      841 gacatgctac ctgaagatac taagcacatt cgatggtttt tgaatattaa caataacggc
      901 acttatgttg aacaaccagt taaaatcagt gatgaaatcc aaagtggaca aagattagat
      961 ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac agaaggttta tagaggagaa
     1021 gagggaatcc aacaattttt acaagatttc cccagcgcta catttaattt ttcagtaact
     1081 gataattaca ttgaaataac tataccaaaa aactttgtca atcttaggaa aataatggtg
     1141 agttataaga cgatcataga aaacccagaa caaataaatt ttgagaatca ttcggaagct
     1201 tggtttaaag aatttaataa acctgctgtt gatggagaaa gtttcaatca tacagttaaa
     1261 aatatttcag cttctggtgg agtcaacggt acagttagag gcgaattgaa gattttcaaa
     1321 tatatcaatg acacagagat tggtatccca aatgtgactt tcgaattaag acgagcagat
     1381 gaacagccta ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca gggagaagct
     1441 agtatcaaag gcctgcaagt aggagactat gttgtaaaag aaaaagaagc acccaattgg
     1501 attgattttg atccactaag ttcaaatgaa ctcaaattct ccataaacga aaatgataca
     1561 gagggagtca gtttacctat ttataacaag aaaaaggtta cgaacatcac agcaactaaa
     1621 atttggaacg gtggaaccac tccaagaccg agtatttatt ttaaattatt cagagcaagt
     1681 acaaacaatt gggaaccagt tcctgatgca gaaacaaaac gattagacaa tggaataact
     1741 tcagtgactt gggaagatat ccaacagtat gatgatagtg gaaacgaata tacgtttaaa
     1801 gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt caggttatca aaaaattgaa
     1861 aacgggctag tcgttacaaa tgagaataaa gaagtcattt cagtctcagg acaaaaaaca
     1921 tgggaagaca atgagaacca agacggcaaa cgaccgacga cgatcacagt aaatctatta
     1981 gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa aagatgattg gcgctatcgt
     2041 ttcacaaatc tgccaaaata cagagacggg caagaaatca tctatactgt aacggaagac
     2101 aacgtgcccg aatacagtac gacgattgac gggtataaca tcaaaaacag ttatacaccg
     2161 gaaaaaacaa atatatctat accaggtagc catataacac cgtggaaaaa ttttccagag
     2221 aagacagaga gagagaaaat tagaaatcta ttctctcaaa aattgcaaat atttgctcat
     2281 acaggagaat ccagcgaaca aagaagcaat tatattagta agagagctgt acctaaacag
     2341 ataacaaata aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc tagttttgct
     2401 gtaatcattg gtttattgat agtaactatc tgtgctggga tattcttaca aaaatataaa
     2461 aaagtttagt tagaatataa tattttttgt taaatatgac tgaaatcaaa agaataaaat
     2521 tccgatttat aatcgaaaaa agaagcattt ttatatatgc ttcttttttc gtagaatcaa
     2581 taaatatgga tttggctaac tatggaagaa tactcgtatt agttttcagc gttcatacta
     2641 gttgttacat tgtttgattg gccaaaagaa tttctgatat attttgtgct aaataaagaa
     2701 aaagacgctt gaattgggaa ataagaaaac tactctatca ttacaaattt tcataaactg
     2761 gcagatgttc taagtaaaca agtatctttt caaagtcgga aaaatccata atcaacgtag
     2821 tggtattgat atttggatga aagcctacgg tatcttgacg atcaatagca gcatcgatca
     2881 cgacttggat ttttttctct ttgtcgtgca tcaatgctaa tggagtcact gtacctgctg
     2941 gaacgcctag gaggtcagga agctgttctt cagaaacaaa agataagcgc ttttctttta
     3001 attgttcagc tagttgtttt aaatcggcgt gcttttcatc aggcaagatg acaagataga
     3061 tttgttttcc ttttcttgat ttcaataata gattttttac ttgcggacca ggtagggaaa
     3121 aagggacatt tttgactgac gtaatcgcag gatgatcgac tttttgaaaa gagataccca
     3181 gtttttttag tagttcataa gcttcttgtt cggttgcacg actcatcgcg catcaccctc
     3241 ctttttttat tagtgtatca taaaaaagag ttgacaaatg aaaaataatc atgtaaatta
     3301 cacgtaaagt taaaacaaat gacatcgaaa agaagagtag ccgttaggtg tttttagaga
     3361 gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa cacatcttgg agtcgatttt
     3421 ctgaagcgta tagtaggaaa atccggggag cccgttacag ctgcggtcgt tgctagatga
     3481 ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa aggtggaacc acgtgattaa
     3541 cgtcctttga tcttcggatc aaaggacttt ttgttttcat ctgaacggca acgactgtgt
     3601 gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac cacgtataat acgtcctctt
     3661 ggcatttgcc aagaggattt ttttatttta aaggaggaaa agaagatgga gtatttgatt
     3721 gaagttatgc cgcaattatt agtaggagca ctcacaacat tgaaattatt tttatttacc
     3781 ttgattggct cattaccatt agggattgtt ttagcattag gattgtcttt gcattttaag
     3841 ccgctacgct acttgcttca aacatatgta tgggttatgc ggggaacacc gctgttgttg
     3901 caactgattt ttatttttta tgggttgccg acgattggga tcgtttttga tcgtttcgaa
     3961 gcagcactca ttgcctttat cttaaactat gcagcttatt ttgcagagat ctttcgtgga
     4021 gggatacaat cgattcctca aggtcaatat gaagcagcgc aagttttgca attaagtccc
     4081 tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa agattgtttt accttctgta
     4141 gggaatgaag ttatcaactt ggtcaaagat tcttccttga tctatatatt aggtttaggt
     4201 gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg cgactttact gcctttgcta
     4261 ggcgtggcaa tcatctatct tacattgact ggtattctaa caatcataat gaaacaagtt
     4321 gaaggaaaat taaattatta ccgctaactg ataggaggaa gaaatatgat tgaattgaag
     4381 aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag atttgaatct aatcattcca
     4441 gatgggaaaa cattagctat cgtaggtcct tccggtggcg gtaaaaccac gcttttacgt
     4501 atcttggctg gcttagaaag ttctgacagc ggagaatttt atttagacgg tgtaccgttc
     4561 aacccattcg agcttaaaat gaaagaacaa gttgtaggag tcgtattcca agacttccag
     4621 ctatttccac atctgaatat ttttgaaaac attaccttag caccaaaaat ggtattgaaa
     4681 cagacaaaag aagaatacac caaaaaagca gaagaattag cggcttcatt agggattagt
     4741 gatttattga atatctatcc ttatcagctt tctggcggac aaaaacagcg ggtagcgatt
     4801 gccagagcat tggctatgaa tccacgggta ttggcttatg atgaaccaac aagtgcttta
     4861 gatcctgaat tacgccagca agtcgagcgt ttgatcctat cattgaaaga agatggggta
     4921 acccaaatta ttgtcaccca tgatttagta tttgcagaaa atgtcggtga tcaattacta
     4981 aaagtttccc caattcatta agaaaggaga agatagagat gaaaaaaacc agttttctat
     5041 tcgctttgat cgcaggtctt cttctttttg caggctgttc gaacaaaaaa acttcagcag
     5101 ataattgggc gactatcaac gaagagaaaa aaatcgttat aggtttagac gatacatttg
     5161 ttcctatggg atttagagat aaagacggga agctcacagg atttgatatt gatttagcca
     5221 aagcagtctt tgaacaatat gggattacgg tagactttca gcccattgac tggtcaatga
     5281 aggaatttga actgaacaat ggaacgatcg atttgatatg gaacggctat tccaaaacgc
     5341 cggcaagaga aaagaaagtg caatttacaa aaccttatat ggaaaacgat caagtgctga
     5401 tcacacctaa agaatcagga atcacaaact ttggacaaat gaataataag agattaggtg
     5461 cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca accagaagta ttaaaagaga
     5521 tagtcaaaaa ccaagatgct gttttgtatg atagttttaa tgaagcattg atcgatctga
     5581 aaagcagacg ggtcgatggt ctgctgatgg ataaagtata cgcagactat tatttgaaac
     5641 aaagaaacga actagaagac ttcaacataa caaaaggttc ttatgagagt gaggattttg
     5701 cagttggtgc acgcaagtca gatagtacat tagtgaaaaa aatcaatgaa gccctagatc
     5761 aactagagaa aacaggtgaa ttccaaaaaa tatccgataa atggtttggc gaagatgtta
     5821 cgccgagaaa ttaacaaata ggggactgtg acaaaagacg tgtcacagtc cctttttccg
     5881 aataaacagt gttctcctgc caactccttg tgactcggag ctgaatggca ggctcacaac
     5941 ctcttggtaa acggtgttca aaagccagtt tttcggtatt aagccaaatt tatgcaaaaa
     6001 tgtagaaagc cattttcaca aaatttttct tactactcga aactaagcga ctttttcaca
     6061 acctccttta cataatttta ttttgttgtg gaaggggttt cgtgttattc taaaagagga
     6121 acgattgaag gttataggaa aggaagagat gaatggaaca caaagaactt acctcatttc
     6181 caaaagattt tctgtggggc tctgcttctg cagcgtatca agtagaagga gcttggcaag
     6241 aagatgggaa aggagaatca gtctgggatc gatttgtacg gatacctgga aagacattta
     6301 aaggaacgaa cggtgatttg gcagtagacc actatcatcg ttataaagaa gatattgctt
     6361 taatgaaaga acaaggtctg aaagcttacc gcttctctgt agcttggaca cggatttttc
     6421 ctaatggacg aggagaagtc aatcaagctg ggctcgcttt ttatgagaga ttgatcgacg
     6481 aattgattga aaacgatatt gaacctatgt tgactttata tcattgggat cttccacaag
     6541 cattgcaaga tgaatataat ggttgggaat cacgtcagat cattgaagat ttcactcatt
     6601 atgcagaaac attatttgag gcattccgcg ggaaagtcca ttactggatt agtttgaatg
     6661 aacaaaatat cttcacttca ttaggttatt tgttagcagc acatccgcca ggtgtgacag
     6721 acccgaaacg aatgtatgaa gttaaccata ttgctaattt agcaaatgca tctgtcatca
     6781 ataaatttca cgagttagaa atgcctggaa aaatcggacc aagctttgcg tatacgccaa
     6841 attatccaat tgacagtaat cctgaaaatg ttctggctgc tgaaaatgca gaagacctga
     6901 tggccaatta ttggatggat gtctatatgt ggggaaaata tccgattgct gccatgaggt
     6961 tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg cgatgcagaa ttgctagaat
     7021 ctgctaaacc agattttctt gggatcaatt actatcaaac ggcgacgaat gcattcaatc
     7081 cattaaacgg agttggtgct ggaaaaatga atacgacagg aaaaaaaggt tcatcagaag
     7141 aaactggagt tcctggaatg tataaaaaag ttgagaatcc atttgtcgaa cgaacaaatt
     7201 gggactggga gattgaccca gaaggattaa ggatcggact gcgtcgtatc acaagccgtt
     7261 accgcatgcc ggtactgatt acggaaaatg gcttaggaga gtatgacaag ttgacagaag
     7321 ataaacaaat ccatgacgac tatcgaatca attacttaca aagccacgtc aaagccatca
     7381 aagaagcaat ttcggatgga gcagaagtcc taggctattg cacctggtct tatacagatt
     7441 tgcttagctg gctgaacggt tatcaaaagc gatatggatt tgtatatgtt gatcaagacg
     7501 aaacacaaga aggatctttg gaacgatata agaaagacag ttattactgg taccaaaaac
     7561 tgattgaaga aaatggagag aacctttagt tcacatactt aaaacaagaa ggtacggctg
     7621 gatatggccg tgccttttct tgataaaatc atttagaaat taagaaaaac tgtaaaataa
     7681 gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa ctttagttcc cttacttttt
     7741 tttgtataat gaaacttgat gaaatgaggg ataataaatg gcacaaaaga aacggcctac
     7801 taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa cagcagcagg aacaaatgct
     7861 gacgattggt gttgggtttg ccttgatttt atttgctgtc ttcggctttt taaaattagg
     7921 ttttttagga atattgattg caaacggctt tcggatcatt gcaggaaata cttaccaaat
     7981 cctctgtctt ttattagccg tattaggatt ttggattgtc attaagaata cagagttttc
     8041 aattggtaaa aaccgacgct ggtttggcgg catactcttt tatttcggta tcttattatt
     8101 gttacatgcc catttatttg gcaaactcca tactggggaa ccaaatatca tggggacgac
     8161 atgggatttg ttagccagtg atataaaaca aagtcaagta gacaataacg ttggtggtgg
     8221 gatgattgga gcaatccttt atcattttac ttacttcttg attgcacagc ctggaagtta
     8281 tttagcagca gtgctattac tggcaggagg agcattcctg ttcagtaatc tggaaggcta
     8341 tcagctgtta aatggtatcc aatcaattgg agaacgagta caggaattat tggaaggtga
     8401 tcctgccaag caagctcgaa agcaagcggc aaaggaagag agaatgaaac agcgcgcaga
     8461 agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca gaaaaagaag ctgcagaata
     8521 tgagaagaaa aaagcggtga gccagcaaag ggaaaatact gctgatgaat ggcaggaacc
     8581 agaaaataca gagccggagc agctaagttt tgttccaatc gacagttttc aagaaaatat
     8641 ccatcctgcg aatctagaga aaccagtacc agatacacca aaacagacca atacagcaga
     8701 aggtttttca gatgagttgc cagaggatga cggtacctca ttagaatttg agattgaagc
     8761 agaacaggaa aatcaagact atgaattacc gacagttgat ttgcttgatt ctataccaac
     8821 tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat atcggcgtat tagaacaaac
     8881 tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc agcttaggac catccgtcac
     8941 aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt aaaattgtga acttaacaga
     9001 tgatatcgcg cttgctttag cggcgaaaga tgtccggatg gaagcaccga ttccaggtaa
     9061 atcactgatt ggtattgaag taccaaatgg aaagatcagt atggtttcct ttagagaaat
     9121 catcgaagca caaccaaacc acccagataa attactagaa gtacctttag gacgtgatgt
     9181 ttctggacgg gtacaaacgg ctgatctttc gaagatgcct cacttactcg tggctggttc
     9241 gactggtagt gggaagtcag tagcaattaa tgggattatc acaagtatat taatgcgtgc
     9301 taagccccat gaagtaaaac tgatgatgat cgatccgaaa atggttgaat tgaatatgta
     9361 caacggcata cctcatctgt tgacgccggt cgtgacgaac cctagaaaag cagcgcaagc
     9421 cttacaaaaa gtagtacaag aaatggaaga acgatatgaa aaattcgcgg caaccggagt
     9481 aagaaatatt tctggttaca atgaatttgt tcaacagaaa aacttggaaa acggaacgaa
     9541 acatccaacc ttaccattca tcgtagtgat cgtagatgaa ttagctgatt tgatgatggt
     9601 agccagcaat gaagttgaag atgcgattat tcgtttagcg caaatggctc gggcagcggg
     9661 gattcatatg atcctagcta ctcaacgtcc aagtgtggat gttatcacag gaatcatcaa
     9721 agcaaatgta ccttccagaa tggcatttgc ggtatcaagc ggtacagatt ctcggacgat
     9781 cattgattct aatggggcag aaaagctctt agggcgagga gatatgctat tcttgccaat
     9841 gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt tctgaccacg aagtagaacg
     9901 ggtcgttcaa tttgttacag atcagcaaga agctcattat gaagagaaaa tgatgccgac
     9961 agatgaagtt gaaacgacag gtgctccaga acagccacaa gatgagctgt ttgaagaagc
    10021 aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc cttctccaaa gaagatttcg
    10081 gatcggttac aaccgagcag cacgtttagt agatgaactg gaagctcatg gagttgttgg
    10141 cccgtcagaa ggaagcaagc ctcggaaagt attcattgag caggaagaaa catcagaaac
    10201 gttgccggac gataatgaca tgtaaaactt gtgtgatgaa gggttgaaaa acgaataacc
    10261 aataaaaaga caaggcatga tgataccaaa agaaaaaatg gtttttttcc atcttttcta
    10321 atacggtatg atgtcttgtc tttttagttt ttgtgagatt aaagttctat tcgatttatt
    10381 tgtgaagtat tgcattaagg aaatgaatca tgtatagtat gtagcgaaac tttgagaaaa
    10441 agagggaaaa tactttggaa aaacagtcca tttatggctt gacgaatgaa gaattaatca
    10501 attggtttat agaaaacggc gagaaaaaat tccgagctgc ccaagtttgg gaatggttgt
    10561 atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct atcgaaacaa ttgatcgaaa
    10621 aattatcgga gcattttatc atcaatcctc taaaacagat ggtcgtccaa gaagcttcgg
    10681 atggtacagt aaaatatttg tttgaactcc ctgataaaaa catgattgag actgtactga
    10741 tgagacaaga atatggcttg tcagtttgtg tgacgacaca agtaggatgt aatatcggct
    10801 gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga tctgactgct ggagaaattg
    10861 ttgcacagat catgatggtc caacattatt ttgatgagcg caatcttggc gaacgcgttt
    10921 cacatgtcgt tgtaatgggg atcggtgagc catttgataa ttacgacaat gtaatggact
    10981 ttttgcatat catcaatgat gccaaaggat tggcaattgg tgctcggcac attacagttt
    11041 ccactagtgg attagcacac aaaatcaagg aattcgctaa taacggcttg caagtcaatc
    11101 tggctatttc cttgcacgcg ccgaataatg aggtgcgaac atcgatcatg cggatcaacc
    11161 gaagtttccc aattgagaaa ttgatggaag ctgtggatga gtatttagag aaaacaaacc
    11221 gtcgaatcac gtttgaatat atcatgttga atcaagtaaa tgatagacca gagcatgcgc
    11281 aacaattagc agatctatta aaagataaga aaaaattagc atatgtcaac ttgattccat
    11341 ataatccagt cagtgaacat gatcaatatt ctcgcagtcc aaaagctgat gtattgagat
    11401 tttatgatgt attgaagaaa aacggcgtaa actgtgtgat acgtaaagaa catggtacag
    11461 atattgatgc agcttgcgga caattgcgaa gcaagcaaat gaaaaaaaca gttgccaaat
    11521 aatcgtatcc tactcgtttt ttcaattacc ataaaaggag cagttttctt aattaaggaa
    11581 ctgttcaact taaataagag aaaagattct atagcgaaag ctataaagct ttttttgaca
    11641 agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga tgacagcctg cttgatgggg
    11701 gttttagcaa ttatttaact gattttctta tatccttgct ggctttcttg tgtagcatga
    11761 taagtttcta cgtcaagaat ggcctgttta aatttttctc gggtgatttc ataaggcaca
    11821 tgtttaatat catttgttgc catggcttta tctaagattg cttctaaatc ggtcgtgaca
    11881 ttgagattca tgtcggttaa cgatactgga agttggttct ttttcataaa cacatagatt
    11941 ttatcaaaca aatcgaactg ctgatccatc atcaataagg tcagtacacc ataactgaca
    12001 gatgctccgt gaaggtatcc ctccattttt tctagaacag tcgtaccgta atagaatgag
    12061 tgtgccatag cagaattata gttattgtta actaaaacag aaaccaatcc ggtagtaaca
    12121 atgatatccg aaacgacttg ttcaattgca tatgagggac gatcgtcggc actatctttc
    12181 aatgcttgcg taccataagt caagagacga tcaatgcaca tttttccgat ttggattcct
    12241 aatctatccg tgtagcctag atcatcgcca cgagcagaaa aagtagattc gacttctttg
    12301 gataaagcat cgccaatacc tgcccataag tatttatcag gtgcttcggc aataattgct
    12361 gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga agagtccgtc cattacgcct
    12421 tcttcgttat agatgacaca taccgcagtg acgggcgaac agttcgaacc aatagtggga
    12481 aaggtgaaga ctggtttagc tagtttttca ccaatgactt ttacagtatc gcaagcacgg
    12541 ccaccaccga ctgcaaacag gacgtctgct ttttggacac tttcatgctg gatcaactgt
    12601 tcaatatttc cataagttga attacccccg taccagagac tggcgacaac ctctatttcc
    12661 tgtgtcaaag ctacttttat tttgttttct gcctttgaaa gtgcagtctt ccctccaatc
    12721 agcactactt ttgtcccata ttctttgacg attccatttg ctttttcata cgcatctgtt
    12781 ccaatggtat aatttggtaa gaaaacactt tgactcatat tcatttcctt ctttcttcgt
    12841 tgaaacgggt taaaatttta aagagtgatc gatctgactg cggctgtaga tggagtagag
    12901 gtgaacctaa gacaggataa gtgatgaata aggtggcatg aacaccttga aatcatcaat
    12961 aagtatccta aaaacgaaca agtacaaaaa cattgaaaac gacaataatg aatttttgtt
    13021 cctcttagta tttctaccga ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa
    13081 ttaagattat tatatgaaaa caggttttat ttgtcaattc ataattggtt ttttttaaag
    13141 caaaagataa tctatatcat gatgaacaaa cagagttttt tcctccaatt gatggctttc
    13201 ctgatctttc ctattttgaa taaagtgttt ctgtataatt tttttatttt ataagtaaga
    13261 taaataatga aagaatagag atactgattg gtagctaaag atttttgagc gagaacattt
    13321 cgttttttca aaaggactgt tagaataagc aagaacaagg gggaagacgt atgataatca
    13381 gtgaatttga ccgtaataat ccagtattga aagatcagct ttctgattta ctgagactga
    13441 cttggccgga agaatatgga gacagctcgg cagaagaagt agaagaaatg atgaatccag
    13501 aacgaatcgc ggtagcagcg gtagaccaag atgagttagt aggatttatt ggtgcaatcc
    13561 ctcaatacgg tatcacaggt tgggaattgc atccattagt tgtagaaagc tcccgacgaa
    13621 agaaccaaat aggtactcga ttagtcaatt acttagaaaa agaagtagct tccagaggag
    13681 gaatcacgat ttatttaggt acggatgatt tagaccatgg aacaacgtta agtcaaacgg
    13741 acctgtatga gcatacattt gataaagtgg cttccatcca gaaccttcgt gaacatccgt
    13801 atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct
    13861 gggacaaacc ggatatttgg atggcaaaaa cgattattcc tcgaccaaat tctcaataat
    13921 aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata agaaggaatt tacgaaaatt
    13981 acttcttgaa tttttgtgaa attccaactt atttccgaag aagctacttt tggaacaccg
    14041 tgtacc
//
LOCUS       DACEKH010000073        13871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEKH010000073 DACEKH010000000
VERSION     DACEKH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.076"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..824)
                     /locus_tag="IW662_001988"
     CDS             complement(<1..824)
                     /locus_tag="IW662_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0866807.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(797..1477)
                     /locus_tag="IW662_001989"
     CDS             complement(797..1477)
                     /locus_tag="IW662_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866808.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1500..2438)
                     /locus_tag="IW662_001990"
     CDS             complement(1500..2438)
                     /locus_tag="IW662_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ0866809.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2683..3534)
                     /locus_tag="IW662_001991"
     CDS             complement(2683..3534)
                     /locus_tag="IW662_001991"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ0866810.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3688..6261)
                     /gene="mprF"
                     /locus_tag="IW662_001992"
     CDS             complement(3688..6261)
                     /gene="mprF"
                     /locus_tag="IW662_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0866811.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6402..7208
                     /gene="proC"
                     /locus_tag="IW662_001993"
     CDS             6402..7208
                     /gene="proC"
                     /locus_tag="IW662_001993"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ0866812.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7287..8843)
                     /gene="rny"
                     /locus_tag="IW662_001994"
     CDS             complement(7287..8843)
                     /gene="rny"
                     /locus_tag="IW662_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ0866813.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9254..10303)
                     /gene="recA"
                     /locus_tag="IW662_001995"
     CDS             complement(9254..10303)
                     /gene="recA"
                     /locus_tag="IW662_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ0866814.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10412..11653)
                     /locus_tag="IW662_001996"
     CDS             complement(10412..11653)
                     /locus_tag="IW662_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ0866815.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11893..12471)
                     /gene="pgsA"
                     /locus_tag="IW662_001997"
     CDS             complement(11893..12471)
                     /gene="pgsA"
                     /locus_tag="IW662_001997"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ0866816.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12680..13564)
                     /locus_tag="IW662_001998"
     CDS             complement(12680..13564)
                     /locus_tag="IW662_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0866817.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         3967 a         3096 c         2427 g         4381 t
ORIGIN      
        1 tagttatttt ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac
       61 aaagcaataa agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta
      121 gttagtcctg acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg
      181 aagcttttgc ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa
      241 agtgcaccgg caatcgggat catcacagca atagccacac cagtaatcac attgaacatc
      301 atcgacatca aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga
      361 acatacatcg gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct
      421 aaaaagatga cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata
      481 cttgttgctg aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca
      541 gccattaaaa tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc
      601 aatatcacag ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa
      661 aagaatccta acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat
      721 tgtcttctga tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg
      781 gcaagaaacg ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc
      841 tcaccagtcg gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca
      901 ttagaactga tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc
      961 tggattcttc atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag
     1021 caaaaatacg cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc
     1081 gatttcgcat atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt
     1141 tttcagagag acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga
     1201 tcaccgtact aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac
     1261 ttaatttttt tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca
     1321 gtaatcctaa cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa
     1381 tgacaaattc acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat
     1441 agtaaaaggt caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact
     1501 taattgatac tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg
     1561 cctgctttga tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact
     1621 cctgtttcat tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc
     1681 actttgatgt tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt
     1741 tcctgatctg gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa
     1801 taagcaaaag cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg
     1861 gagtattttt gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa
     1921 gtttcctttt tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt
     1981 atagctaata ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct
     2041 tcttctgatt cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct
     2101 gcttcgatca tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat
     2161 tccatgtctg aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt
     2221 gcacttggtt cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct
     2281 tcatccccta cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc
     2341 aaatcctctt ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca
     2401 cctatcatta ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac
     2461 aaatcggaat tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc
     2521 aatagaaatc catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg
     2581 atgagttgct gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt
     2641 aggattcctc agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac
     2701 taaataaact tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata
     2761 atgttgacac aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc
     2821 gtacttgatc atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc
     2881 ttctaaactg tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc
     2941 agcaagtccg tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt
     3001 acgtgtagag acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg
     3061 taatacttgg atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta
     3121 aggaacatct gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag
     3181 attccataat tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa
     3241 tccagctgct actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta
     3301 tttatttttt agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa
     3361 tgctttattt gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc
     3421 tgccaagtct acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata
     3481 taatacatcc agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc
     3541 acccattcga aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct
     3601 tatgtctgcc tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt
     3661 cataatccaa acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact
     3721 cggaacattt cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa
     3781 attacttcct ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt
     3841 cttttcataa gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat
     3901 gactttttct tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc
     3961 tagattggct tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata
     4021 gtcagctaat tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt
     4081 cttcgtcatc ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc
     4141 gacaccgcta gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg
     4201 tgcaccttga agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag
     4261 ttgatcgtac caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg
     4321 actcagccga gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt
     4381 gaagtgaaaa cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc
     4441 aagctgatag cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat
     4501 tttaggattg ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc
     4561 aaaaacgact tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg
     4621 aagctgatgg cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt
     4681 ttcgtactca ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa
     4741 agctagcaat gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa
     4801 caaaataaaa tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc
     4861 aattgcgtaa acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca
     4921 agaataaacg aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat
     4981 caagacatat accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc
     5041 aaaagatagt aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct
     5101 agcaaggccg agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa
     5161 tgagaacggc aataatactt gaaatagccg actgattgca gacagattag tgattgttga
     5221 tagaagtacc atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac
     5281 cttttggaaa aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg
     5341 tgtgatgaac aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata
     5401 taatagccaa actactgcta catcttgccg aacaggcaat tgactcattc ctaagatcat
     5461 caaaacgtca aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc
     5521 aatcatgaac ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat
     5581 gatcaaaaat accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag
     5641 caagcctttt ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa
     5701 aatgactggt gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact
     5761 ttctgactga ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt
     5821 cagcataaat agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt
     5881 tccataaaaa ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt
     5941 gatcgtgttt gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc
     6001 ttgtttttct aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag
     6061 cagtccagcc agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca
     6121 aatatcttgc caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa
     6181 cacggagccg aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat
     6241 caagtatcgt ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc
     6301 ccaatgttat cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga
     6361 tagaaaaaga aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg
     6421 gcacagggaa tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga
     6481 aagaagaggt gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa
     6541 ctggcgtcca tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt
     6601 tagccgtgaa accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga
     6661 aagtcttatt catttctatt gcttcaggaa aatcattaac agatttagaa acagcgcttg
     6721 aagatcctca ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag
     6781 gcgtttctgc gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga
     6841 tgatttttga agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca
     6901 ttggccttgc tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga
     6961 ctggtgtact gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta
     7021 tgggttcttg tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag
     7081 tttcttctcc tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca
     7141 tcccagcaat catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata
     7201 aaaaataatg atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg
     7261 ccgcggctct tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga
     7321 ataacgatta ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata
     7381 tcacgtacta aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga
     7441 acttcgcgtc cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata
     7501 ttttccagac ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt
     7561 cttgctgctg agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc
     7621 acatctccat gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca
     7681 gctaattccg caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca
     7741 atatcatgga gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct
     7801 aagataccag ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta
     7861 gttctgaatc gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg
     7921 acttcaaacg ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat
     7981 ttctcgacca tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc
     8041 aaggtcatcc gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc
     8101 ggtgtatcat cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga
     8161 ccttctcgtc caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca
     8221 gtcgtttctg aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc
     8281 gctttgcgat ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac
     8341 tcatgattca gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat
     8401 aaaccagcaa ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt
     8461 tctcgctctt caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag
     8521 ttctcgcgtt tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa
     8581 cgattctctt gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa
     8641 cggtatttct gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct
     8701 tctttttttg cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc
     8761 tcgtgacgtg actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata
     8821 gcgaggagaa tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca
     8881 tctatacagc ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg
     8941 tgtacaaaaa acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa
     9001 taaacaaaga aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac
     9061 tagtttcata taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag
     9121 atgaacagat atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt
     9181 tattcaataa aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg
     9241 tttatcataa tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg
     9301 ggacagcgac ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca
     9361 tcatttcagg atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat
     9421 cttctttata agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt
     9481 ctagaagttc tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt
     9541 tgaaaggagg tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat
     9601 ctgtaccttg tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt
     9661 tcaatgcgcg tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac
     9721 ggatttggtt gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac
     9781 gcaatgcttg agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt
     9841 cgatttctgc tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc
     9901 cacttgatac taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa
     9961 gaagtagttc atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct
    10021 cagcatcaat gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg
    10081 caactgttgt tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt
    10141 aaccgccgac acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt
    10201 gttggtcgat tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt
    10261 ttttcagtgc agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta
    10321 tatttacaaa tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag
    10381 caagaaaaaa ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa
    10441 gatcaaatcc ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat
    10501 atttttccga aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt
    10561 gaccttctaa cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg
    10621 ctatctcacg tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt
    10681 ctgctaatag ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa
    10741 cgaatccgcc tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg
    10801 cgccggctgt caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca
    10861 ctacgacttt tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt
    10921 cttggatttt ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt
    10981 tcactgtcag tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga
    11041 tctgtgtctc aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa
    11101 agcgcaaaac tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac
    11161 gtacttgttc cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg
    11221 catgtgtatc tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa
    11281 ccccgccttc gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg
    11341 tttcaaaata tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga
    11401 ggtgagccgc agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata
    11461 agagtatgag ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat
    11521 tatctcctac aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca
    11581 agaaagtggc attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta
    11641 tttctgcttt cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt
    11701 tatttaaaaa gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat
    11761 aaacagtgtt ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa
    11821 gctgtttttt gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc
    11881 actaaacgat acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac
    11941 acctgaatag atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc
    12001 gatcaggctg aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc
    12061 cgtcttgact tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa
    12121 acgcaagcct gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc
    12181 ctttccttgg tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg
    12241 gtccgcaaac ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa
    12301 ccagtctgtg atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag
    12361 tgtggtgtct cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa
    12421 aatcgggatc atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac
    12481 tcctttcttc cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt
    12541 ccaaatgaat ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag
    12601 aaggaccgcg acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg
    12661 cgtaaactaa tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac
    12721 tctgattgtt ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat
    12781 tgcttgctgc accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct
    12841 gtgtttcatt tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta
    12901 accatacacg attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat
    12961 ttactgcgat ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac
    13021 ttgattttgt tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg
    13081 aagaggactc ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg
    13141 gatcagcttg acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa
    13201 ccaatcctaa taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct
    13261 gatgcattgc ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc
    13321 ctgtcaattt gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag
    13381 cttcagcgta ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat
    13441 catcattgtc gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat
    13501 ctaaagatat ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag
    13561 ccactcgttc atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa
    13621 gacgttatgt ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac
    13681 attgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg
    13741 ttttttgaac ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta
    13801 aacggtgttc aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga
    13861 tatttttccg t
//
LOCUS       DACEKH010000074        13693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEKH010000074 DACEKH010000000
VERSION     DACEKH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.077"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(221..1177)
                     /locus_tag="IW662_001999"
     CDS             complement(221..1177)
                     /locus_tag="IW662_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ0866818.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1281..1883
                     /locus_tag="IW662_002000"
     CDS             1281..1883
                     /locus_tag="IW662_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0866819.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1971..3470)
                     /locus_tag="IW662_002001"
     CDS             complement(1971..3470)
                     /locus_tag="IW662_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ0866820.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3486..4010)
                     /locus_tag="IW662_002002"
     CDS             complement(3486..4010)
                     /locus_tag="IW662_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866821.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4308..5057
                     /locus_tag="IW662_002003"
     CDS             4308..5057
                     /locus_tag="IW662_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ0866822.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5048..5833
                     /locus_tag="IW662_002004"
     CDS             5048..5833
                     /locus_tag="IW662_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0866823.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5802..6518
                     /locus_tag="IW662_002005"
     CDS             5802..6518
                     /locus_tag="IW662_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0866824.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6620..7639)
                     /locus_tag="IW662_002006"
     CDS             complement(6620..7639)
                     /locus_tag="IW662_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ0866825.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7658..8716)
                     /locus_tag="IW662_002007"
     CDS             complement(7658..8716)
                     /locus_tag="IW662_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ0866826.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8937..10085)
                     /gene="nagA"
                     /locus_tag="IW662_002008"
     CDS             complement(8937..10085)
                     /gene="nagA"
                     /locus_tag="IW662_002008"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ0866827.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10161..10997)
                     /locus_tag="IW662_002009"
     CDS             complement(10161..10997)
                     /locus_tag="IW662_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0866828.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11181..12398)
                     /locus_tag="IW662_002010"
     CDS             complement(11181..12398)
                     /locus_tag="IW662_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0866829.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            12781..12864
                     /locus_tag="IW662_002011"
     tRNA            12781..12864
                     /locus_tag="IW662_002011"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12815..12817,aa:Leu,seq:gag)
     gene            complement(13104..>13693)
                     /locus_tag="IW662_002012"
     CDS             complement(13104..>13693)
                     /locus_tag="IW662_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866830.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         4140 a         2794 c         2365 g         4394 t
ORIGIN      
        1 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
       61 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
      121 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
      181 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
      241 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
      301 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
      361 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
      421 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
      481 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
      541 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
      601 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
      661 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
      721 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
      781 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
      841 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
      901 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
      961 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     1021 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     1081 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     1141 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     1201 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     1261 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     1321 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
     1381 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     1441 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     1501 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     1561 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     1621 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     1681 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     1741 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     1801 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     1861 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     1921 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     1981 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     2041 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     2101 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     2161 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     2221 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     2281 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     2341 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     2401 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     2461 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     2521 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttga
     2581 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     2641 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     2701 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     2761 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     2821 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     2881 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     2941 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     3001 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     3061 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     3121 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     3181 ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     3241 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     3301 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     3361 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     3421 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     3481 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     3541 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     3601 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     3661 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     3721 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     3781 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     3841 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     3901 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     3961 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     4021 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     4081 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
     4141 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
     4201 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
     4261 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
     4321 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
     4381 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
     4441 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
     4501 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
     4561 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
     4621 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
     4681 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
     4741 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
     4801 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
     4861 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
     4921 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
     4981 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
     5041 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
     5101 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
     5161 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
     5221 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
     5281 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
     5341 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
     5401 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
     5461 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
     5521 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
     5581 agtattcatc gtgaccgtct gcgatgacgg tgtcgggata tcagaagaaa tctcagaaaa
     5641 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
     5701 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
     5761 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
     5821 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
     5881 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
     5941 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
     6001 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
     6061 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
     6121 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
     6181 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
     6241 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
     6301 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
     6361 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
     6421 tattgaacga aagaatcaaa tatttggaaa tcggccggcc gctaacctgt tatgaattga
     6481 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
     6541 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
     6601 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
     6661 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
     6721 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
     6781 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
     6841 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
     6901 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
     6961 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
     7021 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
     7081 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
     7141 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
     7201 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
     7261 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
     7321 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
     7381 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
     7441 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
     7501 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
     7561 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
     7621 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
     7681 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
     7741 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
     7801 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
     7861 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
     7921 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
     7981 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
     8041 aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
     8101 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
     8161 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
     8221 ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc
     8281 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
     8341 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
     8401 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
     8461 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
     8521 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
     8581 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
     8641 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa
     8701 tgtcaaataa gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc
     8761 ttttattttg ctaatacgta ttaacatttt taattttatc tttacattgt tacttattga
     8821 acaataggaa attaaagaaa cttttgaaac ttatgtaaag tgatttttac tagaacacaa
     8881 aaaaaacctt aaaaccaaac ttaagaagtt tagttttaag gttaacttaa attatattat
     8941 gcgtggaaac gttccacgcc atctagataa gttgcttcca atgtcatatc agggttcaat
     9001 acgataaagt ctgcatcgcg tccttgagcg atttttccac atgtatcatc gattttacag
     9061 ctgattgctg gaatcagact agccatcatg actgcttgtt ctggtgttgc taatccccag
     9121 tctacaacat ttttaattgc ttctttcaat ttcaatatac ttccagctaa gttgccagat
     9181 tctagacgtg ctgttccttc ttttactact actggaaatt cgcctaaatt gtaatttcca
     9241 tcaggcatcc caccggccat catacagtct gtaattaatg cgacatgatc atgtcctgct
     9301 ttttccatta ggattcccgc agcatttgga tgtacatgat ggccatcaca aatcaattca
     9361 gaaaagacat gttctaaaga taataacgca cccaccatac ctggttcgcg atgattcaat
     9421 ccacgcatac cattgaaagc atggacaaat acacttgcgc ctgcttcaac agctttttgc
     9481 gcttcttcta gtgtagcatt actgtgtcct agagaaacaa ctactccttc ttcagtcact
     9541 tgtttgacaa actcttctac accttgacgt tccggtgcta aagcaatttt tttgattaaa
     9601 ccacctgaag cttcttgcca ttcatggaaa gtatccaaat caggatcccc gaaataactt
     9661 gggttttgtg cccctttgtg ttcttctgtg aagaaaggac cttcaaaata gattcctttg
     9721 attttagctc ctggtgcttc ctgatacatt ttgccgattg tctcagctac atcttttaaa
     9781 cgttctttgc ttgacgtcaa tgttgttggt aaaaatgaag tcactccaca agataacaga
     9841 ccttcagaca tcactttgat gccttctgca tcattatcca ttacatcatg attcatatag
     9901 ccgtgaatat gtgtatctac caaaccagga gcaatccatt tgccgctttg gtcaatgatg
     9961 ttgacttctc cctcaggtag ttctttggtg tattcaccaa aaagtccatc ggtaatttca
    10021 agatagcctg catgtttgac acctgacgct aaaaagaatt tgtcagcata gataaaagtt
    10081 ctcattctat tctctccctt tgtttttcta cctctaaagt actcttattt agagaagaag
    10141 tcaagaatgg tctacaccat ttacaaattt tttgaaattt gtttcacagc ttgtgctaag
    10201 ccttcataga atgctgctct ttctggttct tctttacgct tgtatattaa gcttttaagc
    10261 acaaataaat cattgaggta atagtagcta ttcttctctt gagcccaata gattccttga
    10321 ttcacctgtt ctaacgcttc gtcgatttca ttttgatgga ataagaaatt tccataatta
    10381 taaaaaattt gcgaaaattc actttttgat cgttcatcag ctgattgatg cagcagttgg
    10441 atgcttcgac tcaaatagta gcgtgcttca gccgtttttc ctgccacacc atatattttt
    10501 ccgatacaac taatcagttg tatttctgtg tcagaaacat ggattctttc tttttggcta
    10561 gagtagctta atgcttcttt caaataataa agggactgag agatattttg acgcaacgtg
    10621 aattcgcaaa caccgagata ataataatag cgttgaaagt cagttgcttg atacagattt
    10681 tcagaaatat cagaagattt tagtacttgt tctaacttcc gataatcacg tttttggaaa
    10741 caactatcca acagttcaaa taatttatcg tttgtccgag tagtttttcg agagagattc
    10801 atcacatgat caatcgttac ccctagccgt tgacaaatct gcatcatcac caatacattc
    10861 ggcagttcct cattattttc gatccgactt aatacacttt gtgaacaaat atcatgcgaa
    10921 agcatttttt gcgtcatttt ttgttctttt cgaatctctt taatgatcgt tccaaaggac
    10981 tcgattaacg tttccataag tatcctcccc aaaatatgcg tattcgcatt ctattcgtat
    11041 tttactttag gaagtcgcaa acttcaagaa aagacttgca actaaataat atactttagt
    11101 tatttaagaa aactccacta gaaagagagc ctcagtgtgt ttgtctgtgg ccctcctact
    11161 gaaaaatttg gtttttaaaa tcatttttgg cgataggttg ctaggaattc tctcattttc
    11221 ttagctgttt gtttcaaatc atccatttta ggaagataaa cgattcgaaa atgatctggc
    11281 tggttccaat taaatccacc accatgtaca agtagaatat gatgttcatg taaaaagtcc
    11341 aaaacgaatt tttcatcatc caaaatattg aatcgctttg tatcaatttt tgggaatata
    11401 taaaaagcag cttttggttt tacggctgaa agaccaggaa tatcattgat tgcattatag
    11461 atatattctc tttgttcata gatccttccg cctggcagga gcagctcatc gacactttga
    11521 taaccgccta gcgcggtttg gataatttgc tgagacaata cattggaaca caaacgcatg
    11581 gaggacaaca tattcagccc ttctatatag cctttgacgt gtgacttatc tccacttagg
    11641 accatccagc ctactcgaaa tcctgctaca cgatgagatt tggatagacc gttcagcgta
    11701 acaacgaaca aatcaggcgc taatgtagca atcggaatat gttctagacc atccatcact
    11761 aagcgatcat agatttcgtc ggaataaata atcaggttat tttgtcgagc tacttcaacg
    11821 atttcttcta ataattcttt tggataaagt gccccagtag gattattagg attaatgata
    11881 acaatagctt ttgtccgact cgttattttt gatttgatat catcaatatc aggataccat
    11941 tctgcctctt catcgcaaat ataatgaaca ggtgtgcctc cagctaatgc aatagaagcc
    12001 gtccacaacg gatagtccgg cattggaacc agtacttcat ctccattgtc cagcaacccc
    12061 tgcatacaca tcgtgatcaa ttcgctcact ccgttgccgg tgtagatatc attgatcgtc
    12121 acattcggaa aatgtttcag ttggcaatat tgctcaattg cttttctagc agaaaagatc
    12181 cccttggaat ctgagtagcc ttctgagtca cgggcattca tgatcaaatc acgaatgact
    12241 tcgtttggtg cttcaaatcc aaaaggtgcg ggatttcccg tgttcaattt taaaatacta
    12301 atgccctctt cttgcatccg ctccgcttct tctagaacgg gaccgcgaac atcgtaactg
    12361 acaccttcta atttgttgga tttttcaaat tctctcatca cttagccaat ccctcatttc
    12421 aaaaagttag aactttccaa attttttgtt atttactatt tcaatatcat actccatcct
    12481 agaaaatttt gccacttatt taggaaaaac gtaagtatta aaaaaacaca aacaagcgtt
    12541 gagaacttaa ttgtcatgat agtcattcca tagattcgct gcccaatcca ttgcaattcc
    12601 acaaatgatt cctagcataa aaccaagcaa aaaacgtccg aatgcaagtc caatcaatac
    12661 acctaagatc attcccagtc ctgaaaattc tccgtcatat ttctccatct taataagtcc
    12721 tcccattttt gagcttgaca actatgtaaa acctcgctat cattagtgtg ttattattac
    12781 gcagatgtgt tggaattggc agacaagcat gattgagggt catgtgagcg ttagctcgtg
    12841 tgggttcgag tcccatcatc tgcactacaa ctcgtgaagt taattttgct tcacgagttt
    12901 ttttgtttga acgtttcttt atgccattat atacatatca ctgtatcctt tacaaaaaga
    12961 acgtacaatc atgtaaacag cctgaggtga gtatatgaag gtcggaaaca agctactcgt
    13021 agaaacttgc aagtgttaaa acaattaatc gaaaaacgag taaaatccct cacctaatac
    13081 taaagcgagg gattttttgt atattatctg tttatcaccg ttattataaa ggtacaaata
    13141 ccaagcacgg ttaatccaaa ttgcataaat tcattttttt gtacactcat gcttttcatt
    13201 agctctttaa ctgccattag tactaagatg gcgataacaa cataaagtat atagtacgtc
    13261 cgcattccgg aagcccatac tgtgggagaa aatcccataa ttaaccttga acaaaatccc
    13321 attatcatca aaataactaa aaaaatagaa gttttcttat tgtcaaaact taaataaatt
    13381 ccaacaatta aacatagtaa taatgccaag actactaaag tagctatcca agtacctgga
    13441 taaaaaagtg acagtttcgt tcctaactct gtaaagagat tattcaggtt actactactc
    13501 caaatcattg cccttttatt tccattgacg taagtaaagc tttgacccat tgtattacca
    13561 aaataaataa tcaggtttaa gaaaaacggt aatgcagtca atatacgcac ataatagttt
    13621 tgacatttcc tatatgttag aaggaaaatt aagaaaaata gcaataaaaa taagatattc
    13681 atgtctaaaa aaa
//
LOCUS       DACEKH010000075        13274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEKH010000075 DACEKH010000000
VERSION     DACEKH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.078"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            241..1980
                     /gene="yfcC"
                     /locus_tag="IW662_002013"
     CDS             241..1980
                     /gene="yfcC"
                     /locus_tag="IW662_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0866831.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2137..3321
                     /gene="iadA"
                     /locus_tag="IW662_002014"
     CDS             2137..3321
                     /gene="iadA"
                     /locus_tag="IW662_002014"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ0866832.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3432..3635
                     /locus_tag="IW662_002015"
     CDS             3432..3635
                     /locus_tag="IW662_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0866833.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3736..4398)
                     /locus_tag="IW662_002016"
     CDS             complement(3736..4398)
                     /locus_tag="IW662_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866834.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4411..5913)
                     /locus_tag="IW662_002017"
     CDS             complement(4411..5913)
                     /locus_tag="IW662_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0866835.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6461..8140
                     /locus_tag="IW662_002018"
     CDS             6461..8140
                     /locus_tag="IW662_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0866836.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8267..8746
                     /locus_tag="IW662_002019"
     CDS             8267..8746
                     /locus_tag="IW662_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ0866837.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8965..9645
                     /locus_tag="IW662_002020"
     CDS             8965..9645
                     /locus_tag="IW662_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ0866838.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9653..10984
                     /locus_tag="IW662_002021"
     CDS             9653..10984
                     /locus_tag="IW662_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0866839.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10999..11961
                     /locus_tag="IW662_002022"
     CDS             10999..11961
                     /locus_tag="IW662_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ0866840.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12072..12410
                     /locus_tag="IW662_002023"
     CDS             12072..12410
                     /locus_tag="IW662_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ0866841.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12489..13196
                     /locus_tag="IW662_002024"
     CDS             12489..13196
                     /locus_tag="IW662_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ0866842.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4253 a         2330 c         2899 g         3792 t
ORIGIN      
        1 acgagatttt ctctaatcta aaaataaaaa aagaatttta aggaaaaaca gagaaataaa
       61 atacttaata taaatggaaa gagaaaaaaa taatcataca taggagaaaa aaaacaaaat
      121 agaaaaaagt gattttctaa taaattattc atgtggaaat gtcattttta ctgtataatt
      181 catttgatta tccatttttt attcatcaaa attactgaaa aaagaaagga agaaataaat
      241 ttgaagaaaa caaattcttc aggaaaaccg aaacgcgact tcactaaatt atcgacaccc
      301 catacgtatg tcattatttt tggtgtagtc atctttgcat ggatactgac ttttgtcgtt
      361 ccagcaggaa agttcagcac gcaagatatt gaatacaaag atgcaaatgg cgaaacaagt
      421 actcgtactg ttttacgtca agattccttc cgttatgctt acgagctgga taagtcgtat
      481 gtgtttgatc aattagaaga actgcaagat catccagcag aacgtgaaaa actggatgtt
      541 ccggaaaaag gattggaaaa agtcattgct gacggagaaa aaaatcttac gcaggaaaaa
      601 ctagatgaaa tctccttgac agatgatgtg ctttacgatc agtacggcga aaatatctat
      661 gatacttcta aaaaattgca taaaacagcc aaaatatggg gaacagatga ttttggcggc
      721 tttggtttct taaattttgt ttttgaagga ctagtatctg gagataagta tggatctgct
      781 gtagggatcg ctgcattgat ccttgttgtt ggaggtgctt ttggtattat tatgcgtacc
      841 ggagcgatcg acgcaggtat ctatgcgttt atcagtaaaa ctaagggact ggagcgttta
      901 gctttaccgc tattattctt tgctttttca ttcggtggcg ctacattcgg gatggcagaa
      961 gaggtaattc cattctcaat ggtgatggtt ccatttgtca ttgcgttagg atatgactcg
     1021 atcgtagcag taaccgtgac ttatgtcgca tctcaagtcg gaaatgcgac ctcttggatg
     1081 agcccgttta gcgtagcggt ggctcaaggg attgctggaa tccccgtgtt gtctggtgcg
     1141 acattccgat tgattatgtg ggtagttgtt actgcattag ccgctggtta tatgatgata
     1201 tatgcagaaa aaatccgtaa aaaaccagaa agttcgttga cttacaaatc agatgattat
     1261 ttccgttctc atatcaaaaa gacatctgat gaaaataaaa aattcatgct tgggcataaa
     1321 ttgatcttat tagagatgtt agttgtcctt gtctggatcg tttggggcgt gactcaaaaa
     1381 ggctattaca ttccagaaat cgcttctcaa ttctttgtga tgggattagt tgctggaatc
     1441 attgctgttc ttttcaaatt agatggaatg aaagtcaatg acatcgcttc ttctttccaa
     1501 tctggtgcag ctgatttagc tggtacagca atcgtagtag gtatggccaa agggattctt
     1561 cttgtcttgg gaggatctga tgcatctgtt ccttcagctt tgaatacgat tttgcatggg
     1621 atcgggacgg cgttgaccgg tgttccagct gtcattggtg cttgggcgat gtacatcttc
     1681 caaagtttgt tcaacttggt tgttacatca aattcaggac aagcagcttt gactatgcca
     1741 atcatggctc cactggcaga tttagtaggt gtatccagac aagtagcagt tcttgcttat
     1801 cagcttggtt caggttttat ggacgctttt acacccgtat cagccagctt gattggtgtg
     1861 ctaggtgttg cacggatcga atgggcaaaa tgggcaagat tccaaatcaa gatgcaagga
     1921 ttcctgtttg ttttaggaac tattttcatc atgattgcca ttgcaatagg tctgcagtag
     1981 tactggagac ctgtaaataa aaaagtaatc caaacagtag ataaggactc caagaaccgt
     2041 cattttacgg taaaatcaaa ggagtaaaga tcctatcgtt tggattattt tttaagatcc
     2101 tatcatcagt tatacttaaa gaaagaggaa aaatgtatga aaatcattcg acaaattgaa
     2161 gtgtttgcac cagattactt aggaaaaatg gatgtactga ttgccggtgg gaagattttg
     2221 gctgtagaag aacagctaga aggcggctac gaaggagtgg aagtagaaga actttcaggt
     2281 gagggaaaag tactaacccc gggtttcatt gactgtcatt tccatatttt aggtggagga
     2341 ggagaaggcg gttatcaaaa tcgaacgcca gaagtcacat tgagccaatt gactacagct
     2401 ggtgtaacga cagttgtcgg ttgtttggga acagacggag aaggtagaga tatgactgcg
     2461 ttgatcagca aagcgaaagg tctggaagca gaaggaatct ccacttatgt atacgaaggt
     2521 tcgtaccgat tgccggtaaa aacagtaaca gattctatca tcaaagactt tttgacaatt
     2581 gataaaatta tcggaatcgg agaaatcgca gtatccgatc accgttcttc tcagccaagt
     2641 tttgaagagt tcgctcgtgc agtagcagat gctcgtgtag gaggcatgtt atccggaaaa
     2701 gctgggatca tcaatgtcca cttaggcgga ggaaaaagaa agctagagct attgacacga
     2761 atcgtagaag aaacagaaat tccgatcaca caatttttgc ctactcatgc aaatcgcaca
     2821 ccagaattat ttgaagcttg cgtggcattt gccaaacgtg gtggcacgat tgacttcact
     2881 gctagcgaag atcctgattt ttgggagaag acagatggtg aagttcgctt cagcaaagca
     2941 ctgaaacgat tgatcgagga agatgtttct ttagataatt tcacgatgac ctcagatgga
     3001 caaggaagct tgccttattt tgatgaaaac aatcatttcc tagggttagg agtaggaagt
     3061 gccaaggcat tgctagtagg gatcaaagaa gctgtgcaaa aagaaagtat ccctttggaa
     3121 atcgccttac gagctataac cagtaatcca gcacgtatat tgaaattgga taaaaaagga
     3181 aaaatcgaaa ttggtgctga tgcagatctg tgtatcttgg ataaagaaac gcttgatatt
     3241 gatacagtta tcgctaaagg agaaataatg gtccaagaaa aagaagtcaa agtatggggg
     3301 actttcgaaa aaagttttta atcaacagct ctaactatct tttttagaaa acgagcgttc
     3361 tgtttgtctg tatctgttgt tgatgttacc ctgaaggtag atcaataaac aaactaagag
     3421 gaggaagcac aatggcagat ttaaaaggac gttctaaaga tatgaaagat aaagtagtcg
     3481 gtgaaggaaa agaagcatac ggcaaagcaa cagacgacaa gagcaaagaa acagaaggga
     3541 aagcacaatc actaggtgct gacatcaaag aaaaagctcg aaatttaggc gacgaagtca
     3601 aagacaagtt ttctcgaaaa gacgaaaaaa aataaaaacc acttattact aattgaatca
     3661 agcaaataaa aaatccgaac catatctgcg agacagatat ggttcggatt ttttatttga
     3721 tcattatcct gttttttaaa cttcgatttt ttgaaatttt ttatttttta gttcaaagac
     3781 ttcatcacag ctttttgcta cttcaatctc atgagtcacg atgataatac agcgattttg
     3841 ttcatgggcg atttcttgga aaagtttgac gatatcttgt gaagttgttt catctagatt
     3901 tccagtaggc tcatccgcta cgattagttg atgttcacag cagatagcac gaacgatagc
     3961 gactcgctgc tgctgacctc ccgaaagttt ggtcacctgt ttttttgcta atatttcatc
     4021 aattcctaca cgagccagtc gatctaaagc aacttgtcga taattctctt gcttggtctt
     4081 agtgatttcc attgctgcta tgacgttgtc tagagccgat aaataaggaa gcaagttgta
     4141 agcttggaaa ataatcgaga catcattttt acgataactt cttaacccga ttttgttcag
     4201 tgaacgatct tgatagtata cctttccttc ttttggtgca tctagtccag aaataagcga
     4261 aagaaaagtc gtttttccag aaccacttgc tcctaaaata gcgtacattt ttccagcttc
     4321 gaaattcagt tctacatctt tataaagcgc atcttgcgga tcttgatacc aaaatcccaa
     4381 ttgttctgtc cgtaacatca aattcctcct ttagtttaat aggatttttc ttgggtttaa
     4441 tcttaatatc cctatagaag ctaaaaagac agaaaacaga ctgattccaa atgctactcc
     4501 ggctaatagt cccaattgtg caggtttgac attgatttcc aattcgctga cttgatcaga
     4561 aacagcaaat ggattattcg tggttccttg ttgaccatcg cgttgaccgc cttgttcttg
     4621 tctgttacct ggcatttgtc caccgccagg agcttgctgg ttttgtgtcg tggtttctgt
     4681 ctgttggtca agtagctgct gtcccacggc attcgcgacg acgtttccgc taagcgaagc
     4741 aactcctaat gcaagaatca tacaaatagc cacttcggta aacaattgga gaatgatttt
     4801 acttctggat tctcctaaag atagcaagac gcctaactca tgccgacgct cccgaatggt
     4861 aatcataatg atcagagtaa gtatgataat accggctaca gccactagta aaacaacatt
     4921 tttagaaaaa cttgccacgt tgtttaaagg ttccaacatt gactgataca tggaatcatt
     4981 tgattgtaag ctaaatgtat ctgtgtcgat cagcttctca gcttctttga gaaactcatc
     5041 cattttatct ggatcagata gagtgtaaat agcagaatct atcgtgttat cgtcagatgt
     5101 cccatttagt tcatttgcaa aagtgtaaga agtgtaaagt gtattcgacg gattcatgaa
     5161 gttgaatttc atacccatac tgccgcttgt ttcgctggat tcataaattc ctttgatcgt
     5221 catttcataa gtggtatctt catcttcagt actcgtgatt gtgaaagtat ctccgactgc
     5281 caggtcattt gtttctgcta gagtagaatc aatgactaca ttgtttgtgc ctttgtcgct
     5341 gtcatctatt ccttcaccat ccgtgatttt tgctgttcca tcagaaaaat tactattttg
     5401 tgcagttgct gacacaccac tgatttgaaa gtctgcttga gtcatttgcg gattcccgcc
     5461 cattcctcca gcaggttcac cgcctgcatc agtagtttct gaactgtctt cacttgaatc
     5521 agaactagtg atggcagtta ttccatcttt tgccatagct gaggcggaac tttcaaagga
     5581 ataactcttt acattatcta attcagctat tttttccgcg tccgttaagg aaactggtgt
     5641 aagagaaaaa ctgcctggat ctggacgttc ttcagtggta gaaccgcttg aagaagtttg
     5701 ttcctgtttt ttaaatgaag cttctcgatt ggcacttaaa gtcactgttg ctccaacttc
     5761 tttttgtgct tgattcgctg cttcttctgc agcgcttcga atcgtgaggc cagctagcac
     5821 aaaaataaga atggctgtga acacggcaaa caataacaat gaacgtcccc attttacttt
     5881 tgtacttttc catgcacgtt tcataaaatt catattttgc atccctccta gtagatgcat
     5941 ttgataatag acaacctatc ttaaagctag cttaaatctt atgctgtttg cacaccatca
     6001 acatgaggtt ctgtatatat aaaaagtcac tatagttatt gcaaaaaatg tggtaaaatg
     6061 aaaaaagtat gtttcaaacc gacgaagaag aaatgaaaga ggaatagcaa aagaaatcct
     6121 agatgctaca gagggatttt tacgtgcata gttgttctaa caattgctga tactagcgat
     6181 ttagtttatt tgttgtataa taatggggaa cagctgaata cttatataag tattctggct
     6241 atagcgtttc gcaaaagatc cattttgtta atgaagtata tccaaaataa aatgcccatc
     6301 caactgtcag aacgcggatg tgctggtaat tttcctgctg attctccata agttgagaaa
     6361 tagtgatttt gcatatgctt cattggaatg gctcatatat ttagataaag aagaaaatcg
     6421 acatctttaa acttggatgt cataagaaga ggtgaagttt gtgagtacca ttaaaattat
     6481 cccattaggc ggcgttcgtg aaaatggaaa aaacatgtat gtcgcagaag tgggcgagga
     6541 tatttttatt ttagattgtg ggctaaaata tcctgaaaat gagttgttgg ggatcgacgt
     6601 cgtaatccct gattttacgt atttagaaga aaacattgat cgtgtagctg gtgtcttttt
     6661 aacacatgga catgcagatg cgatcggggc acttccttac ttgttgtcaa aaatttctgt
     6721 accagtattc gggacagagt tgacgatcga gttagcaaaa ttgaatgtca gtcgtaatga
     6781 agcagccaaa ggatttaagg atttccatgt ggtagatgag catacagaaa tagactttgg
     6841 tgaaacagta gtgagctttt tccgtaccac tcatacgata ccagattcag tggggatcaa
     6901 tctgaaaaca aaagaaggta gtatcgttta tacgggagat tttaaatttg accaaacggc
     6961 tatcccaatg tatcaaacag attatgcacg attggcggaa atcggcaaag aaggagtctt
     7021 agccttacta agtgaatcgg caaatgcaga aaatcctcag cctgtagctt ctgaagccca
     7081 aatagcagat gaagtatttg atacgatcaa atattgggaa gggcgtatca tcgtcgcttg
     7141 tgtagcaagt aatttgcaac gagtgcaaca agtattggat gcagcagaaa aagcacaacg
     7201 aaaagttgtc ttgactggtc aagatttcga acgaattatt cgcacagcga tgcgtttaga
     7261 aaaacttcaa ttaccaagtg aagatctgct tgttacgttg aaagacatga aaaactatga
     7321 tccagaagaa ctgatcattt tagaaactgg ccgaatgggt gaacccatca agtctctgca
     7381 aaaaatggca aacggtacac accgtaatgt caagattgaa gaaggagatt tggtttatat
     7441 cactactacg ccaagcatcg cgatggaaac catcgtggcg aaaacggaag atatcattta
     7501 tcgagctggt ggaacagtga agttgatctc tgagaatatg cgcgtttctg gccatgccaa
     7561 tcctaatgac ttgcaattaa tgatcaattt gatgaaacca acatattttg tgccagttca
     7621 aggagagtat cgtgaattag ctgctcatgc agatttggct catgcagtag ggatgccata
     7681 caaaaatatc tatattactg gacgcggaga cgtgctggaa tataagaaca agcgtatgag
     7741 tgttgcagga actacaagtg cagaaaatgt aatgattgat ggattaggca taggtgatat
     7801 cggaaacatt gttttacgtg atcgaaaaat cttgtcagaa gacgggattt tcgtagcggt
     7861 tgtcactatc agtcggcgag aaaagaaaat catttcaaaa cctcaaatta cttcacgggg
     7921 attcgtttac gtaaaagcta gccgtgatct gatgaaagaa agcagcaata tgattgaaac
     7981 aatcgttgag aaacatttag aaagcaatga ttttgagtgg agcaaattaa agcaagacat
     8041 ccgagatcaa ttaggccgtt acttatttga acaaacaaaa cgccgcccag tgatcttgcc
     8101 agtcattatg gaagctactc aacgtcgaaa taaaaaataa acaaaaagag taagatgaac
     8161 ctctctatag agtgctcgtc ttgctctttt tttgcgaaaa acaaataatt tttgaataca
     8221 gcatccctcg ctttataata aacgatagaa taaagggagt gaatcaatga tgagtcaaac
     8281 aaaagtcgaa cgttatttag aagaacaaaa gatcagctat caaccacttg attttagtca
     8341 tgtggtagaa gaatctttca cagaagaact acaaaaaaga gggattgatc cagctttgat
     8401 atgtaaaact ctcgtagtga aaggaaacaa aactggggtg atcatcgcat tgattccttt
     8461 gaacgatcac ttagattaca aaaaaacgag acaagtgacg aaagatcgta aagttggttt
     8521 tcctggaatg gattttgtat taatgcatac aggatatccc cacggagcaa acacccctat
     8581 tggaataaaa ctcgcacatc ctgattattg ttttttagca gacagttctt taaaagaaaa
     8641 agaagaagta attgtgtcaa gcggtgaaat cgggagaagc cttcggataa aagtagccga
     8701 tttagaaaag atcgtccatc ctatttacgc tgatttaatt agttaaggga cgagaagaga
     8761 agctaagcag agcagagacc tcaaacttga gattatatct gcttggtact tggtgcatcc
     8821 tgtattatct gttacaattg ctttgtacat actgtactat caatccaatg aaaatatatt
     8881 tagcagctat attttttcat tgatgtgcaa gtgatgaaat cgtttttaat aaaaaagata
     8941 cagagaaaac gcgaggtgta aaatatgaaa attacgtatc atggacattc ctgtttaaca
     9001 attgaaatgg aaaatggcca acgcgtgatg attgacccgt ttattacagg aaatccattg
     9061 acagacttaa ctgcagaaga agtaaagaca gattggttgc tgattactca tggtcatagt
     9121 gaccacatag gagatatgat tcctattgca aaagaaaatc aagccacaat tatcagtatt
     9181 gttgagattg ctgattatgc gaacagcaga ggagtagatt cattcggtat gaatatcggt
     9241 ggaaaacatg cgtttccatt cggcgtggta aaattcgtac atgcacagca tagttcaagc
     9301 tatgaagtag acggtatcgt tcagtatatg ggcgagccta gcggaattat tattcaagca
     9361 gaaggaaaaa cgatttatca tgcaggggat actgcttatt tcagtgatct aggattgctt
     9421 gcagaagaat tcgatattga tgtcgctttc ttgccgattg gcgataacta tacgatgggg
     9481 ccagaagatg cagcaaaggc tgcgcaaaca attcgtgcca agaaagtagt tccaatccat
     9541 tacaatactt tccctgtcat tgaacaagat cccgaatctt ttgtagagtt actggaagat
     9601 agtgaaggaa aaataatgag cgtctcggaa acgctggaag tgtaggcaga aaatggcgac
     9661 aaaacatgat ttgattttag aatatattga aagtttacct gttggcaatc gaatttctgt
     9721 aagaagcatc gcaaaagaat tgaatgtcag tgaaggaaca gcgtaccgtg caatcaaaga
     9781 tgcagaaaat gtcggactag tttcaacaat tcaacgagta gggaccatcc gtatcgaacg
     9841 caaattaaaa aaacatattg aacgtttgac atttggcgag gtcgtcagga tcattgaagg
     9901 ggacgtttta ggcggggcgg caggattaga caaggtcttg aataaattcg tcatcggcgc
     9961 gatgactaaa catgccatgg aacgctatat tacgccagga tcactaatga tcgtcggcaa
    10021 ccgggcggaa gtccaaaaat tagcattgga agatggcgcc gcagtattga tcacaggagg
    10081 attcaatacg actgctgaga ttgcaaagct agctgatgat cttgaaatgc cgatcttacg
    10141 cacctcttat gatacattta cagtcgcaac catgatcaac cgagcgttaa gcgaccaatt
    10201 gatcaaaaaa gatatcatgc ttgtcagtga tatctatatg ccagctgaaa agactcatta
    10261 tttgcagcag acagatacga tttctgatta tcagcgtttg tcagaagaaa cgcagcattc
    10321 gcgttttcca gtcgtcaacc gccatcatcg tttgatgggg attgttacgg caaaagatgt
    10381 tttagggaaa tcgccaaacc agatcatcga tcgggtgatg acgaaagaac caatcagtgt
    10441 aaagaaaaca atgagtattg cctctgttag ccatcaaatg atctgggatg ggctagaagt
    10501 catgccggtc gtttccgatg acttgacatt ggaaggcttg attacccggc aagatgtaat
    10561 gaaagcgatg cagcttgtcc agcgacaacc gcagattgca gatacgattt cggatcagat
    10621 atctggaaat atccatacga ttgatactga ccgtgaagga aatcgtttgg aaaaccctaa
    10681 gttcaaattc gtagtagcac cacaaatggt caatggcgtt ggaaccattt cttttggcgt
    10741 acttagcgaa atcatttctg atgtcgcaca aaaaacaatg gtgatgaacc aaaaacgaaa
    10801 tatcttgatt gaacaagtga acctacatta tttgcggctg atccaattgg aaagcgaatt
    10861 agacattcgt ccacgtgtat tggaaatcgg cagacgttcc gctaaactgg atatcgaagt
    10921 gtttattgaa aacaccatcg tttccaaagc aatcgttgtt tgtcaagtaa tggaacgttc
    10981 ttagaaagca ggagtgaaat gacagtacag caagaaatag tagatacaat aaaaaaatac
    11041 gataaaatca ttatccacag gcatttgcgc cctgatcctg atgcattagg atcacaagta
    11101 ggattagccg aactattacg ggaaagtttc ccagaaaaag aaatacgtca agtaggtgaa
    11161 acgattgaag ggcttcgctt tttagcagaa atgcaagaag ttgaagatgt cttctaccaa
    11221 ggagcgctag tcatcgtgac tgatacagct aattcaccca gaatcagtga tcaaagatat
    11281 cagttggcag aaaaactgat caaaatcgac catcatccca atgatgaccc ttatggagat
    11341 cttgtgtggg taaatacaaa agcaagtagc tgtagtgaga tgattgcaga atttgctttg
    11401 atgtttcctg aatctttaaa gatgaacgtt aaggctgcac ggttacttta tgcaggaatc
    11461 gtcggagata cagggcgctt tttgtatccc tccacgacgt cccgtacatt agagattgct
    11521 tcgatattga gaaattatcc atttgatgca agtgctttga acagagagat tgaacaaatg
    11581 ccgatgaaag tcgctaaact ttccggttat ctttatcaaa atatccaggt ggatgaaaat
    11641 ggtgcaggaa gaataatcct tccgcaagaa attcttgata aatatggaat cgatgattca
    11701 gagacagctt cgatcgtttc acttcctgga gtgattgacg aagtgctggc ttgggggatt
    11761 tttgttcagc agccttcagg gtattaccgc gtacgtttac gttctaaggg gccgatcatc
    11821 aatgaattgg ctaaacgcca tcatggagga ggccatccat tagcaagtgg tgcgaatgca
    11881 aaagatttag cagaagtaga ggaaatctat aaagagatcc aagatatatg ccaatcgtat
    11941 aagagcagaa aaacagaatg atacttcgtt cgaaaacgtg ccttttgtag taaaaatcac
    12001 aaatgtatct tttcaagatc ttctttttcc gttacaatag tgctcactaa agattttaaa
    12061 ggagaaagaa gatgacatta ccaaattgcc cagaatgtgg ctcagagtac acatatgaag
    12121 atcgaggctt gttgatttgc ccagaatgcg ggaacgaatg gaacaaagaa gaacaagaaa
    12181 ctgtcataga agacggatta gtggtattag atgcaaatgg aaaccgtctg ttagagggcg
    12241 atagcgtgac agtgattaaa gacttgaaag taaaaggtgc gtcaggtgcc attaaacaag
    12301 gaacaaaagt caagaacatc cgtttagtag aaggcgatca taatatcgat tgcaaggtag
    12361 aaggatttgg accgatgaaa ctgaaatcgg aatttgtgaa aaagaattaa agaatattca
    12421 aggtcttttc acattctttt cctactacta atgataaaat aaagtaaacg aaaaggagtg
    12481 aaacgaaaat gtatggttat ggaatgtatg gttttttaga tccgacattt cttttggtca
    12541 ttcttggatt agttatttcc ggaattgcgt cagcgtacgt caatagtact ttccgaaaat
    12601 acgatcaagt gagaagcaaa aacaatgtga ctggtacaca agcagcacaa tatatattac
    12661 aaagtcaaca gataaataat gtcggtgttc aacaaattgc cggagatctc acagataact
    12721 acaattcagg taataagatg ttgagtttgt ctcaagcaac tgcacagtct acttcagttg
    12781 ctgcgatagg tgtagcagct catgaatgcg gacatgcggt acaagatgct tcgggttatg
    12841 tcccattgaa gattcgtgca gcacttgttc cagcagctaa ctttggttcg atgatttcgt
    12901 tccccttgat tatggtaggt gtattgctta gctggaactc tacgttaatc aatatcggga
    12961 ttttcgcttt ttctttagca ttacttttcc aattggtgac attaccagtc gaatttaacg
    13021 cgtctcggcg agcaatccaa attttgagtg aaggcggact actgacggaa gaagaagtac
    13081 cgatggcaag acacgtatta tttgcagcag cactgactta tgttgcagca gcgctttcga
    13141 cattcctgca gctgttgcgc ttgatcttat tgttcggcgg caaccgaaga gattaaaata
    13201 aagaagttgt gaaatcagcg ctttgatctg agctaatacc gagagttagc tgattgaata
    13261 ccatttattt ggaa
//
LOCUS       DACEKH010000076        13212 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEKH010000076 DACEKH010000000
VERSION     DACEKH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.079"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            147..1016
                     /locus_tag="IW662_002025"
     CDS             147..1016
                     /locus_tag="IW662_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ0866843.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="IW662_002026"
     CDS             1225..2250
                     /locus_tag="IW662_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0866844.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="IW662_002027"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="IW662_002027"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ0866845.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="IW662_002028"
     CDS             3333..5036
                     /locus_tag="IW662_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0866846.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="IW662_002029"
     CDS             5038..7071
                     /locus_tag="IW662_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0866847.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="IW662_002030"
     CDS             7082..7522
                     /locus_tag="IW662_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0866848.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="IW662_002031"
     CDS             complement(7722..8849)
                     /locus_tag="IW662_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0866849.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="IW662_002032"
     CDS             complement(8852..9529)
                     /locus_tag="IW662_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866850.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="IW662_002033"
     CDS             9793..11559
                     /locus_tag="IW662_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ0866851.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="IW662_002034"
     CDS             11574..12011
                     /locus_tag="IW662_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0866852.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="IW662_002035"
     CDS             12024..13175
                     /locus_tag="IW662_002035"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ0866853.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DACEKH010000077        13133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEKH010000077 DACEKH010000000
VERSION     DACEKH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.080"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(93..635)
                     /locus_tag="IW662_002036"
     CDS             complement(93..635)
                     /locus_tag="IW662_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ0866854.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            770..1159
                     /locus_tag="IW662_002037"
     CDS             770..1159
                     /locus_tag="IW662_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866855.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1163..1927
                     /locus_tag="IW662_002038"
     CDS             1163..1927
                     /locus_tag="IW662_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0866856.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2056..3003
                     /locus_tag="IW662_002039"
     CDS             2056..3003
                     /locus_tag="IW662_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0866857.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3133..3873
                     /locus_tag="IW662_002040"
     CDS             3133..3873
                     /locus_tag="IW662_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866858.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4164..4832)
                     /locus_tag="IW662_002041"
     CDS             complement(4164..4832)
                     /locus_tag="IW662_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0866859.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4966..5253
                     /locus_tag="IW662_002042"
     CDS             4966..5253
                     /locus_tag="IW662_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866860.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5669..7207
                     /locus_tag="IW662_002043"
     CDS             5669..7207
                     /locus_tag="IW662_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ0866861.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7204..8169
                     /locus_tag="IW662_002044"
     CDS             7204..8169
                     /locus_tag="IW662_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866862.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8156..9478
                     /locus_tag="IW662_002045"
     CDS             8156..9478
                     /locus_tag="IW662_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0866863.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9541..9645
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9719..10462
                     /locus_tag="IW662_002046"
     CDS             9719..10462
                     /locus_tag="IW662_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0866864.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10477..11469
                     /locus_tag="IW662_002047"
     CDS             10477..11469
                     /locus_tag="IW662_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0866865.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11481..12218
                     /locus_tag="IW662_002048"
     CDS             11481..12218
                     /locus_tag="IW662_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866866.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12208..12930
                     /locus_tag="IW662_002049"
     CDS             12208..12930
                     /locus_tag="IW662_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0866867.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13014..>13133)
                     /locus_tag="IW662_002050"
     CDS             complement(13014..>13133)
                     /locus_tag="IW662_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0866868.1"
                     /translation="YLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4373 a         2238 c         2707 g         3815 t
ORIGIN      
        1 atatgtcgat gttttttaat caaaataata aataaaaact gtctaacatt taaaatttaa
       61 tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt tctcactttt
      121 ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa agcacgctct
      181 tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga aagtatttcg
      241 agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc ataaccgtgt
      301 tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa aatcgttgtg
      361 accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact gatacctact
      421 gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc taaaacaagg
      481 gataagccga cgatataaat cgtgatctcg accatactga caagtggagc aatcagtcga
      541 tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc aaaattaatg
      601 aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt catttatata
      661 acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga aactttatac atacaaaaaa
      721 atgataaaat gattataata gaattataaa gataggttag gtgattaaaa tgaaaacggt
      781 caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt ctcagtatat acttaggtgt
      841 ctattcgatt caacagccaa ttgaggtaga gaacattgtt tttcagatgt cgctgacaga
      901 tggtttaatt ttaagcgttt ttttcttttt gatactgttt attggtaata ttttcggtag
      961 cgttgccagt tttttgaatt tttctattta tccactcctt tcgctagcat taggagtggt
     1021 gggactttgt tctctgtgct tatttcccga gccattcagt cctgcattta tattatttgg
     1081 tacactgaac ctgttccaga caactgttgg agcatggttg ctatggcgat caggaaattt
     1141 aatgaaaata ggtgagtaac aattgaatcc aattatattg aaacgttctg tccatcatat
     1201 tccagtgtta gaagttgttc tgaaagaatt aagagaggaa cgtattcccg ttgtcatcta
     1261 ttatcacgga tggcagacaa gtaaggagtt agttttgacg caaggaagaa aaattgcgca
     1321 gcagggcatt cgtgtgatcc tgccagatgc gatgaatcat ggggagcgca aacagccggt
     1381 ttcttctatt ccatcctata cattttggga tagtatctac ggaaatctgt ttgaatttga
     1441 cacacttatc aagcatttag aaaaaagagg tcttctacat gatcagcttg cagttggcgg
     1501 ggtttcgatg gggggaatga caacatgcgc cttgttggct aaacatcctc atatagtagc
     1561 gggcgtgtgt ttgatgggtt cccctgcacc tttggaatat ggaaaagaaa tcgtaaggcg
     1621 cgcatcggaa tataattatg tcgtaccaaa agactatttt gatttgattt catgggttaa
     1681 gagctatgat ttgtcattgc agccggaaaa gttagctgga cgtcctctgt tcttttggca
     1741 tggacaagct gacgaaaaaa tcccttatat acaaacggaa cgatttgtag aaaaaaatcg
     1801 gcataagtct tacggaaaag aaattgtttt tcgttcctca aaaacacagc gacatatggt
     1861 acaaatacca ctaatggaag aagcttccac ttttttagca aaaaatttga taaaaaagta
     1921 taaataattg aaacggaaaa tcactcatga cgatagagga ttttccgttt tttattttgg
     1981 gctataataa actctgttag gaaaatttgg tataattgat agtaagaatg acaacatgtt
     2041 agaaaggaag agagcatgcc cactttagca gatgttgcaa aacgtgccaa tgtttcaaaa
     2101 atgacggtct ctcgagtcat caatcatccg gaacaggtga ctgacgaatt gaaagagtta
     2161 gttttctcag caatggctga actggattac cgtccaaata ttgctgcaaa agcacttgtc
     2221 acgaatcggt cgcagatcat caaattattt atactggaag aaatcgacac aactgagccc
     2281 tattacatga atttgctgat gggaatcgct aaacggatcg gtaaagcgca ttattctctc
     2341 caattagtta caaatgatgc cttcgacgtc ggttcttgtg atggatacat catcacaggt
     2401 gtcagaaaaa aagacttcga ttggatcgac cgtttggaaa aaccagttgt cttgtttggg
     2461 gaaaattctg aaggatatga ttatgtagac agcgataatg aatatggtac agcaaaagca
     2521 acgcaatatg ccttagaaag agattatcaa acgattattt atatcggtat cgaagtggac
     2581 gagccttttg aaatttcaag ggaaactggt tatttatctg tgatgaagga agttgatctt
     2641 cctagtaaaa tcttgcggtt tccaaatcat tcgacagaag tagaaagata tatgacagaa
     2701 aactgggcag attttacaga taaaacttgt tttgtatgta gttcagatcg gctagcacta
     2761 ggagcagtaa gagggattat aaatgctgga ggacacttgc ccgaagaatt tggtgtgatt
     2821 ggatttgatg gggtattttt agaccaaatc agttcaccta aattgacaac gatcaagcaa
     2881 gatattatac gtatgggtga agtatgtgga gaaatgttgt taaaaaaaat agaagaaaaa
     2941 ggggcgagtc aaggttatcg tcgttttttt cctgaactag ttatcagaga gacaacgaat
     3001 taagtcttat tactatatat atatttatga aattcatcgc tattttctta aaataatcag
     3061 cgcattgcta ctgaaaacaa tgaatctgtg ctattattga gaaaagttcc atttttgaga
     3121 aggatggttt gaatgaaaga aatcagacga aaacatttcc gttttcctgc gtacgatgat
     3181 gagttaggcg taaaattaac caacgacaag aagcgagtat tatttgacgg acaagaagat
     3241 cttttgactg aacagaatca gtccccgttt gaacagatga aagaatttca tttcacgaac
     3301 acgaacaccg agaaaatccg tcgcaagcgt cgtgcaacaa atggattgaa gtcagctccg
     3361 cgtaaggaaa atttggaaaa gcataaagga aaattaccgg attataccgg ccattaccaa
     3421 tcagaaatca catcgacagg acgaaaaaaa ctttttggtg attcctctcg ttctgtcaga
     3481 caaagtgaaa caaggacaga gtccaagcat actacacgaa acgcatctgt tgacacgaag
     3541 gtgcgggagc gttcttattt tgttcctaaa tatgtacccg catcgattat cccagaccct
     3601 agagaaagca aaatttctga ggatcattta atcgaatcga tgaaaaaaac ggatgactct
     3661 tatctgttgt tcgatacaga accgacgccg tatcaagaaa aaagaaatgg tgagccgtct
     3721 gtacgaacat tcaatcgaac aaaaacgtta gaggaaacaa agcagtcaga agaaaaaaaa
     3781 tcgaaaaaaa gccatggtat cttagaacgc tcattacaag gattgatcga tgatcaagga
     3841 aactctttgg gagaaaatag ctattttaaa taagtgatat acaaatttat attcggctac
     3901 agttggaaag gaaacgctgc ggcgtttcct tattttatac tctatccgaa agtttatatc
     3961 gtatcctcaa atttttttga gtttaagcga attttttctc ccaataagta agaaaataat
     4021 ggcaaaataa aaaaacaaac tattttcgca gatcgtttgc aaaaatagtt tattttttta
     4081 taaagaagat tttactgaat aatatcattt tagtttacat aatgatattt ctgtaaatta
     4141 aataaagtag tgaaaaataa gaatcagtgc tctaactgtt caagaggatc aagtggttca
     4201 aatgtttctg tagtctgttt tgcttccaat tggggcaaaa cagatccaat ttccagatac
     4261 ttcactttat tcaatgtata agatagaata gaacagctaa gtaatgtata cagaaaatgt
     4321 gtgagatcca aataaagcaa ggagacaact aaaataccaa aagtaatcaa gaaaagactt
     4381 ttgccaatct taatatgaaa atatttttca atggcttgat aaatgatatc tgttccgcct
     4441 gtgcttccgc cgaatcgaaa gacgagaccg caaccaatgc cagaaagaat acctgtcatc
     4501 aaactattga tcagtagact atctacagca aaatgtatat gaaggctctc ccataaatgt
     4561 aaaaagaaaa ctagcgcaaa tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa
     4621 agaaacaatg agataagcaa taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg
     4681 aatccaaaaa gattatgcaa aagcaaagca ataccagtaa ccccaccatc tgccagctta
     4741 gatggaatat tgattcctgt cacagctaat ccgtagattc ctgcgcctaa aatgattccg
     4801 cacaattctg taaataaatt taattttttc atttctagcc tcttttcttt tgaaaatttt
     4861 cagcaacttc tttattatac tgacttatac tcttacttca agaggaaaat gaaaaaaaat
     4921 tatttttggc tgtttctttt tatttatcaa ataaggagtg gaaaaatgcg agcagcagac
     4981 ttattgacaa ttcttacaaa atcacccaaa aaaacagagt atcaaggagt ttttttggct
     5041 caagaacaaa aactggtgcc tgtgacaatg gtccagataa ataaagcaaa tcaatttatc
     5101 tttttatttg aacacggcaa agcaccactt gctatgaaag acatctttct ttttttgatg
     5161 aagaaccgtg agaaagctgt ttattatcag tcaaaagaag aactcattcc tctttatgga
     5221 gtgaaagaga tagaaaataa agtggtgata taagtaaaaa taagaaaaca agacgtttat
     5281 gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag tgtcatgata cagtataata
     5341 ttcgttgaac aggccaataa aggtgttctc ccccacgaga tttctacttt attggctgtt
     5401 tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa
     5461 tttcgtatag atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga
     5521 ctttgttgct tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg
     5581 ttcgtgctgt aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata
     5641 cttaaagaat aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag
     5701 aaaaggcgag ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct
     5761 gattgcagca ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc
     5821 agaagtctta ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg
     5881 tttacttatc ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta
     5941 cgctttgctt ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat
     6001 tgaagaagca gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct
     6061 tcccatcgca ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg
     6121 tacggctact ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg
     6181 tattgaccga aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc
     6241 aattatattc agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat
     6301 tactttagca gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt
     6361 taaaaatgaa accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca
     6421 aatgtataaa gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt
     6481 tggcaagacg agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga
     6541 gttcacaggt actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga
     6601 tttgacaaaa gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat
     6661 gacattatta aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt
     6721 tgcgaacgaa catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa
     6781 agcgggattc acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc
     6841 atatggcttg aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat
     6901 caataatgga gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta
     6961 cgatctagtg actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct
     7021 gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag
     7081 cggaaaaata acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa
     7141 aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga
     7201 tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc
     7261 atcgatgacc taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc
     7321 ggtagtggaa aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga
     7381 gacatctact tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat
     7441 atgggatatg tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt
     7501 gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa
     7561 cttcttcaaa aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg
     7621 tctggaggag aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg
     7681 attttgatgg acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa
     7741 ttggttttgt ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg
     7801 gaagaagcaa tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa
     7861 tgtgacacac cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt
     7921 gatgaaccga aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc
     7981 ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa
     8041 gtatattcgc ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt
     8101 gggtcgctga caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa
     8161 aacatatgat gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt
     8221 gaaagaagca ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc
     8281 taaccgtgga tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt
     8341 aaaacaattg agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca
     8401 aaaattcaaa cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga
     8461 agctgaaatc gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt
     8521 caacgaagaa gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat
     8581 cttaccagtg gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt
     8641 actgcctaaa gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat
     8701 tgccaatgct gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga
     8761 atttgaggcg tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt
     8821 tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg
     8881 aaaagagacg gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac
     8941 agaatcttta gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa
     9001 tgattatctg caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa
     9061 aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac
     9121 ggatgcaaca agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc
     9181 taaacttgct gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga
     9241 gatggatctt accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga
     9301 attgaccttt gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga
     9361 cgaattgatc gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga
     9421 tagctatatc atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc
     9481 agcatgataa ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa
     9541 tacctgtttc tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg
     9601 aacctgtcag ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta
     9661 tgtcgtgccc atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat
     9721 gaagaaaaat ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt
     9781 tgtcatagtt tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc
     9841 attagcggca gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt
     9901 gcttggtttg gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg
     9961 gaagttttta gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat
    10021 ggtgttaggg ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct
    10081 tgttgtcttg ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt
    10141 atcaaaagag caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg
    10201 aatatataaa ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac
    10261 ttattgtgtc agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta
    10321 ttacatgata cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg
    10381 tgtcattcta ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta
    10441 tcgtatgctt tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta
    10501 ccgctactgt tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa
    10561 ataaaggatt tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact
    10621 ggcatctacc tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc
    10681 atcgaaccag gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt
    10741 ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc
    10801 aaagcaattg ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt
    10861 aacatccatt caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt
    10921 gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca
    10981 atggtaggaa gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg
    11041 tactttgaag gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc
    11101 ccattgaaag aattagatga acggttagat tactatactc cggtaattat tacgaatgat
    11161 caactgatca aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc
    11221 tatcaggaag ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa
    11281 gaaaatgatc ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat
    11341 gatcctgaga attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg
    11401 caggaaaacg gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt
    11461 ataaaatagg agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa
    11521 acctgaaaat attagaagac atccatttca ctatccctaa agataccttt acatcgattg
    11581 tcgcgccaag cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta
    11641 tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta
    11701 gttatatgcc gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt
    11761 tatatcaaaa aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata
    11821 tctttgggtt aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc
    11881 agcgaacggc cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac
    11941 catttggagc gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc
    12001 ctaaaaaagc aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt
    12061 tatcagacag gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag
    12121 tgcctgaaaa acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga
    12181 tttatcagtt attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg
    12241 agtcagagtt tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat
    12301 tcgccagaag aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt
    12361 tacggtatcc atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg
    12421 aaaaaagcaa ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg
    12481 actacacaaa aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct
    12541 gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct
    12601 aatcactatt tgatccattg gtattcttct ttggactatc aacaagacta tattgattta
    12661 ggctgttatt tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct
    12721 aaagcagtcc caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg
    12781 gctttagggg ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag
    12841 acaatcgctg agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac
    12901 ttggaacgtt tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac
    12961 ctctttttgg ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac
    13021 caaccgtttg cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca
    13081 tattgttctg ctactctttc ttggtttgca gcagagtagt caccatttaa gta
//
LOCUS       DACEKH010000078        12670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEKH010000078 DACEKH010000000
VERSION     DACEKH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.081"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..305
                     /locus_tag="IW662_002051"
     CDS             6..305
                     /locus_tag="IW662_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0866869.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IW662_002052"
     CDS             378..1739
                     /locus_tag="IW662_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0866870.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IW662_002053"
     CDS             1761..2021
                     /locus_tag="IW662_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866871.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IW662_002054"
     CDS             2035..2787
                     /locus_tag="IW662_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ0866872.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IW662_002055"
     CDS             complement(2881..3486)
                     /locus_tag="IW662_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0866873.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IW662_002056"
     CDS             complement(3539..4513)
                     /locus_tag="IW662_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ0866874.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IW662_002057"
     CDS             complement(4891..5670)
                     /locus_tag="IW662_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0866875.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW662_002058"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW662_002058"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0866876.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IW662_002059"
     CDS             7161..8558
                     /locus_tag="IW662_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0866877.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IW662_002060"
     CDS             8639..9079
                     /locus_tag="IW662_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0866878.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IW662_002061"
     CDS             9227..9553
                     /locus_tag="IW662_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0866879.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IW662_002062"
     CDS             9554..10303
                     /locus_tag="IW662_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866880.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IW662_002063"
     CDS             complement(10383..10838)
                     /locus_tag="IW662_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0866881.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IW662_002064"
     CDS             complement(11265..11540)
                     /locus_tag="IW662_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866882.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IW662_002065"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IW662_002065"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ0866883.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2176 c         2586 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttgctct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACEKH010000079        12264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEKH010000079 DACEKH010000000
VERSION     DACEKH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.082"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..282)
                     /locus_tag="IW662_002066"
     CDS             complement(<1..282)
                     /locus_tag="IW662_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0866884.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKD"
     gene            complement(279..932)
                     /locus_tag="IW662_002067"
     CDS             complement(279..932)
                     /locus_tag="IW662_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0866885.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1220..1740
                     /locus_tag="IW662_002068"
                     /pseudo
     CDS             1220..1740
                     /locus_tag="IW662_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1642..2358)
                     /locus_tag="IW662_002069"
     CDS             complement(1642..2358)
                     /locus_tag="IW662_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0866886.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(2482..2667)
                     /locus_tag="IW662_002070"
     CDS             complement(2482..2667)
                     /locus_tag="IW662_002070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866887.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            2894..4167
                     /locus_tag="IW662_002071"
                     /pseudo
     CDS             2894..4167
                     /locus_tag="IW662_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4274..5305)
                     /locus_tag="IW662_002072"
     CDS             complement(4274..5305)
                     /locus_tag="IW662_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0866888.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5312..6142)
                     /locus_tag="IW662_002073"
     CDS             complement(5312..6142)
                     /locus_tag="IW662_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0866889.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6139..6945)
                     /locus_tag="IW662_002074"
     CDS             complement(6139..6945)
                     /locus_tag="IW662_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0866890.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(6951..7775)
                     /locus_tag="IW662_002075"
     CDS             complement(6951..7775)
                     /locus_tag="IW662_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ0866891.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7765..8865)
                     /locus_tag="IW662_002076"
     CDS             complement(7765..8865)
                     /locus_tag="IW662_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0866892.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9043..9828
                     /locus_tag="IW662_002077"
     CDS             9043..9828
                     /locus_tag="IW662_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ0866893.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9861..10244
                     /locus_tag="IW662_002078"
     CDS             9861..10244
                     /locus_tag="IW662_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0866894.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10320..10451
                     /locus_tag="IW662_002079"
     CDS             10320..10451
                     /locus_tag="IW662_002079"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0866895.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10420..10656
                     /locus_tag="IW662_002080"
     CDS             10420..10656
                     /locus_tag="IW662_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0866896.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(10734..11705)
                     /locus_tag="IW662_002081"
     CDS             complement(10734..11705)
                     /locus_tag="IW662_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0866897.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3679 a         2765 c         2227 g         3593 t
ORIGIN      
        1 atctttgatt tgtatttcat tcttattatt tttttctttt atgaatactt ttatttcact
       61 actcttggaa taaaactcca aagtttgttc tatatatttt atttcttttc cattcatatt
      121 actagaaatt tcatttgcca tattatgaat cttttctttt ctcgtctcca aatatgtttt
      181 aggaaagata aaaaaaatta atgaatgaac taatataatt attattccaa gaacagaaaa
      241 tatttgtatg aacatttttg gaaatatctt taattttttc attttctctc caatttatat
      301 cctacatttc ttatagtagt tatacagtct aattgtaatt tttttctaag ttcttttata
      361 tatacatcta ttactcgatc ataaggagtt tcttcgctat ctttccatac ataatcaata
      421 atttgcattc ttgttaacac ttgtccatta ttatccaata aacattttaa tacttctagt
      481 tcttttgcat ttacatctat ttcttcacca tttattttag ctgtatagct attaaaatta
      541 accgatagat ccttatattc aaacttctct aaatgtccat aattcttctt aattaaagaa
      601 tctattctag cttttaagac cggcaatgaa aatggctttt ctacatatcc gtctactaaa
      661 ttagtaaatg catcaatttt atattcttca tcactaaatg cagtcaagat tagaattggt
      721 aaattgcttt tctttctaat ttctttcaac acttctaaac catttataaa aggtatctga
      781 atgtctaaga taaccaaatt tatatcgcta ttaaattttg acaaggcttc tcttccatct
      841 ttagcttgaa taacagtata tccaaattct gaaagatatt cacttattcc ctctcttatc
      901 atattatcat cttcaacagt taatattttc atatatacct ccacttaagc ctatacacta
      961 gatattatac agcatattat ggctctataa tatctagtgt tggtgacgcc ttctgttgcc
     1021 aatacttttt ctttttttta ataaatgcaa acagacacct tgaaatcctt tagaaaaaag
     1081 gcttctataa tattttgtgt tgttggaaaa tcgaaggcga ttttctacta acactttttc
     1141 ttcgtttcaa ctcaaaaaga gactcacttt tggtaaaatt tagtcgacca aaacatcacc
     1201 ctaccaaagg agtcgctcta tggataatca tactagaaaa ttactcggat taacagataa
     1261 acatttattt tttgatgaag aatggctgat tgaaaaagaa tataaaggcg ttcaagcgca
     1321 gtttattaaa gggagattgg atgactcacc ttcccattgt gaacactgtg gatcgattcg
     1381 tattatccgc aacggttcct atacgacacg tactcagatg ttgaaagtca aagaaaagtt
     1441 gactatttta gaattaaaac gtacccgttt tctatgctat gattgtgggc agaccttttc
     1501 tgcgaaaacg gacttagttg atgaacatca tcaactaaca aaggaactga agcaggcgat
     1561 tctcatagaa ctgtacgaaa atcaatcacg taatctgaaa atcagaagaa gtaccgtcgg
     1621 ctcaagcgat attggaaact cttactcaaa gattccacga cattggagcc gctcaaacgg
     1681 cattatcatc gactgtttaa acgaccaatt agtcaaacag aaatcgtcga tgaaccgttg
     1741 tgcacaatgg tgtcctgaac gtacagcgac accttccata tccagagcag tcgccacatc
     1801 acggggatgg atgccatcca aattaaaagc aaccacgcca gtatgatgtt caggatcttg
     1861 cggtccgtaa atggtgatac cgccgatcgc ttgtaattta ggtaaaacgt aggcaaccag
     1921 ttcttgctca taacgatgaa tattctccat accaatctca ttcaagtaat cgattgctcc
     1981 tgcaatgttc ggagtaccag cttcaaattt ccatggcagc tctttccatg aactatcgta
     2041 aagattgaca aaatctatca tttcaccgcc gaattcgact ggctccattt gttctaacaa
     2101 ttgacgtttg ccatataata caccgattcc tgtcggcccg cacattttat gcccgctaaa
     2161 ggcgaaaaaa tctgcatcca acgcttgtac atcaactgtc atatgcggtg ctgcttgggc
     2221 accgactgga ctatcctcaa ctacaacagc ctgttcaacc ctcagccgtt tcaagcacaa
     2281 caaataacct tctggatctg gtttgccgac ctgaagatca tcgatcgtca caaaggaatc
     2341 aattttttcc ttaatcatcg gatgtgcttc aaaaaaatag tctagctccc gcagttcaga
     2401 attgagacta ctccgatttt ccagtgctgc tgatccgctg ctcaaaatgt tcagcatcca
     2461 ctggtctccc aaccgcttga cttatccctt cataaaagga cagttctgct gccgtcccaa
     2521 caccttgttg atagtctgca aaaggaattt tatagccgaa ctgctccagt aaataggctt
     2581 cagtaaatag tgatgaatac tctcgtaaaa catccattca ctgtcaatca tcgtcccatc
     2641 aaaatcaaaa ataagtgctt tggtcatctt ctttccccct tacccaaaac aagcaaaagc
     2701 tgtgtcctgc tctctctaca gtatcagcat aaaaactgcc gtctgaaaag tcaaaggtga
     2761 aagaaatgtg gcgctataat ttctaggact gatagtaaat cattctatca gtccttttga
     2821 gcataaaaaa acaaaacata cagaagctcc gttagaattg aatcaccaca acacaatcaa
     2881 aggagcgttc tgtatgcctg atcttcatct tatcaaacta ttactaggaa tcaaagatgg
     2941 aaacattcaa atcactcatg tcgagaagaa aaaaatacat tcaatcaaca gtatcgttgt
     3001 ttctgccacg ctcattaaaa cgattcagcc ctgtcctcag tgtaaacagg tgaatcaaca
     3061 tgggctgatc gttaagaacg gaaagaaaaa atcgctggtc cttctcaata agtgtgcgaa
     3121 tcaacggact tatcttgctt tggcaaagca acggtacctc tgtcgtggct gtcacaccta
     3181 ttttacggcg aatacctaca ttgttgaaaa aaactgtttt atcgccaaac aagtacatta
     3241 taaaatattg gaggaattga ctgaaaaaca agcaatgacg acgatttcta aacattgcgg
     3301 cgtttcttgg tcaaccgttt cgcgaacact cgcttctctg ctccccatga caaacgtgaa
     3361 aataaattgg ttgcctcgct gttcattggt agatgaattt cgttcattga aaaatcaagt
     3421 cggtccctat tctttttctt gtatggatgg cgatactggg aaattactgg atattcttcc
     3481 ttcacgtaag aagaaagatc tcatttctta ttttatgcaa tttgaacgac gtgcacgttt
     3541 aaacgtaaaa atactcgtaa cagatatgaa cgcctcttat gctttgctga taaaagagtg
     3601 ctttcctcat gccaaactcg ttgtggatcg ctttcacatc gtcaagcacc tcattcgcag
     3661 ttttaaggat atttgactat gagtcatgaa aagcttcgac cgaaacgatc caatacaagc
     3721 caaacactat cgacaagtga aggctttatc tcgacttctg atcaaacgac aggacacgct
     3781 tgtttatgat aaatggacaa aatggcgtaa ttttggttgg gcctatttaa ccgaaagtga
     3841 agtcgtagaa cgtctacttt ctacttctga tgaactacga atagcttatg cttactatca
     3901 agaaattctt caagcttttt atgacaaaga agcggacatc ttctttcagc ttgtaaaaac
     3961 aatgcccaag tcggttccaa gagaactgca tcatataaaa aaagcgttta taaactatga
     4021 atccggcatt cgtttagcct tagagttgcc ttactcaaat ggcaaaatag aaaatctgca
     4081 tacccacatc aaagcattga aacgaatagc ttacgggtta ggatgagaac cagaattttc
     4141 ttaatcaatc atctgatcac ttattaaaca taaaaaaaca tctaaagcag aaaatcaatt
     4201 ctaccttaga tgttcttata tcactctatc agtcctattt gacaaagagc cagaaatgtt
     4261 tgccgactat acactagctt tgttgtgcaa aatgctgagt atcaatttcc acagggattg
     4321 ctagaatatc tttcagctga atgtataagg catcgctaat tttctcttcc cgatcgttca
     4381 accgatccac taaaatttct ttgccctcca gcgtttgaaa aacataccgt tgaatgttgc
     4441 ctaaaataaa tgattccttc catagaaccg gaatcttaac cccctgagga accggttcaa
     4501 attgaatcag ctccggtctt aaataataaa tgcagcgatt gcccttagcg gaaaactcgt
     4561 gggtatactg attcagctca taccattcga aacgattata gttgccgata aattccgcaa
     4621 caaatcggct ctgaggattc tgatacaatt ccttcgaggt ggaaacctga gccacccgcc
     4681 cagcttccat cacaaaaatc cgatcactga ttcgcatcgc ctcttcttgg tcatgagtca
     4741 caaaaatcat cgtcatccct agcgattgct ggatagcgcg caaatcccgc tgcagctgtt
     4801 ttctaattcg ggcatccaaa gcacttaatg gctcatccag cagcaggact ttcggttcag
     4861 tgaccaatga gcgagccagt gccacccgtt gtcgttgacc gccagaaaga aactggacat
     4921 ttttatctgc ctgctccgtc atatccacca atgatagcat ctcaaaaacc ttttcctgaa
     4981 tcacctcagg actcagcttc tttaccttca aaccaaaggc tacattttca aaaacagtca
     5041 tagttggaaa caatgcatac tgttgaaaaa caaagccgat attgcgatcc ttcgtaggct
     5101 tatcggcaat attctcattg tccagcacaa tctcaccgct ctttggttgt tccaatcccg
     5161 tgatacagcg cagcaacgta gactttccgc acccgctggg acctagtaaa gtaaccagct
     5221 cacccttttc aatctgaatt tcatcaatag ctaaaatctg tttttcatca aaaaacagat
     5281 tgagatcatg tatttctaaa aacattttcc gtcacaactc ctttaagggc ttctttttcg
     5341 actgcttctc ttccctcgtc tgcttaaggg acagctgttg cttttgtcga taagtcagca
     5401 taaaggtagt taaagcaatt cccaccataa acagcaaata caaaaccatc acagcgctag
     5461 ccaaatggcc gttttcgttc atccgccgca gcatatagat gcgtaccgtc tgaaatcgac
     5521 cgccaatcaa caggttcgtc agcatgtact ccccaaaaac cgaagaaaaa atcatcagag
     5581 aaactagggt cacaccgata cggatgtttg gaaacagcac ctggataata atcgtagaca
     5641 tcggggcgcc caaggtttct gctgcatcca ctaattcctt cagattaatt aagcgcatct
     5701 ggttgttaag actaagatag acaatcggca acgccgtaat aaacaatgca cctaccaaca
     5761 ctacaaacat tgggatacca gtttcagcat acgttcgaag caaagccgtt accaaaatca
     5821 ctcctggtaa agcatacggc agcaggagca gcttttccag ccaacgattc aaacgaggga
     5881 aatacaaatg aatccaaata atggtgggaa tcatcaacaa cagaatcgtc accaagacca
     5941 cacctgctaa taaaagtgag cgacccaccg cagcaataaa ttccctgtcc gtcagcagct
     6001 gttggaacca ttgcaaggta aaatcactag gtaaaagcga ttttgaccat ttagtggagc
     6061 tggcatacaa aatcgttcca ataatcggca gcaagtaata caggacaaag cagaacaaaa
     6121 tcggttttat taactttttc acgtctgcac caccttgcct ttcttttgaa taatcaacat
     6181 tgccgttacc agcaggacac ctaaagcgac tgccaacgca ctgcctaaat taggcttggc
     6241 gaacacatct cccgacacta aagccgcaat tcgaatcgtt aaaagattta cattactgct
     6301 ggtcagggca tacgctgttt cataggtccc catgccatta gcaaataaga tgacaaaggt
     6361 gctggcaatc gatggtcgca aaaaaggcaa agccaccttg aaccaagcat acgctgatga
     6421 agcgcccagc aattgtgcgg actccagcca ttcctttttc acttccttca tgctgggata
     6481 caaaaacaag acccctaacg gaatttggaa ataggtatac gtgatggtca atccccacca
     6541 agaatacaaa ttgaagtccg ctagaaatgg caaaatcatt ttcagcacac ctacattccc
     6601 caacaaaata ataaaagaga atgccagcgg aatcccagca aaattagctg ccaaattgga
     6661 aacaaaggtg attttctcct gcaatttagt cgatagattc attaagcata aacataaaat
     6721 aaacgtcccg attaacccaa tcacactgga gctgacagca atcagtacgc tgttaacaaa
     6781 ggcctgtgtg taatatttgt tgctgaaaat atcactgtaa ttggccaatg tccactggtt
     6841 agttaaatca ctgtgcaagc tgcacagaac cataaaaagc agcggcagca gtaaaaatgc
     6901 acagaagata aaggaggcag gaaccagaac caagcgtttt ttcatctctt tcatcccctc
     6961 atcaattctt gaatgtcctg cattttctct gacggtgcaa ttcctaataa ccagcaaaac
     7021 aatggagccg ccagcagctg tggtacagtt tgttccttca gcttttggcg tttatttgaa
     7081 atcaaataaa aaggaacttc acgatgtgca gccaagctgc cgccatgcag tccgtgcgca
     7141 tccatcccgt gatccgcagt caccacgact tgataacctt tttgcagcca ttcaggaagg
     7201 taaatagcca ataatctgtc cgcctgatta accgctgctg catattcatg agaatcggcg
     7261 gtaaatttgt gaccgacatc atcaatattc atcgaatgta cgaccaaaaa gtctggctga
     7321 tactgattca ctaaataatg ggcatccgca aataaatgcg aatcagggta agtgtcctca
     7381 ctataaaaga tgccatgctg aatcagtcgc tcctcctgat gctggatgcg atcaaccaaa
     7441 cgattatagg gggcttgatt gtacaactcg ctgtaccagt ggtaagcggc ggctcctgtt
     7501 tttccgccag cttccttaac taattcaaac agcgaccact ctttggatcg ctgagcatgg
     7561 taattactat agatgccatt ttcataggct ggaacgccgg tcatcagtac ttcgtataac
     7621 ggacgtgaat tgctgggcag ctcacttttt accttcaata agcttgcttg ctggtgctca
     7681 actaaatgat tcatgaaccc aagctgggcg acggctgtgt cgtagcgaca gccgtctagt
     7741 ataactacaa tcagtttgtc aagtctactt tgcataggtc agtacctccg cctgccaaag
     7801 ctctggaagc tctacagagg tcttgttcca agcttcattg tcttcaactg gcaccgccgc
     7861 ttcataagca tcctccggca gtaatttttc agctacttct ttaggcagct ctaccttgcg
     7921 aataggtcgc gcatagccct ttgccaaatt gatttgccct tcatcagata aaatatattc
     7981 ccgtgctagc tttgccgcat tgggatgagg ggcattttta ttgattaagg ttgtataccc
     8041 gctaatgatg gagccgtctg ttggcacagt tacctcaaaa cgctcggagt caatttggtc
     8101 acgataattt agtccattga agtcccaaac aactgccaca tcgatttcac ctttttcaag
     8161 atttgctacg ctggcatcaa cggtggacaa acggcctttt tctgccattt ttttgtagta
     8221 attcagacct ggctgaatat ccttttcgtc acctccgttg gcaatcgccg cagccaatac
     8281 agcaaattgt gcttggttgg caatcgtcac atcccccacg ctaacttgat agtcaccctc
     8341 ttgcagctcc ttccagctag tcggtgcttg cttcacattc tctttatcgg tgataaagga
     8401 aatggttccc gtatagctta acaaccaatc cccatcatca tcctttgccc aatccggaat
     8461 ttcctcccaa tagttggtct tataaggtaa ggttaactct ttgctttcag ccaacggacc
     8521 gaagctgata ccgacatcac caatatcggc ggttccattt tttccttctg attcaaactt
     8581 agccaattct tctgcactgg acatatccgt atccacgtga cttaagccgt attctttttc
     8641 aaggtcgttc caagtgccta cccaatttgc ccaagtatcc ggcattccca agctggcaat
     8701 ctctccttct tccttggctt tttctataat cgtatctaaa gagtcgtctg ccgcagccga
     8761 ttgcttcgtt gcttcacctg agacgcagcc tgtcagcagg atacttactg ttgctgcaca
     8821 aaataaaccg atagtcatgt tacgaatttt ttcattcatt ttcattcatt cttctcccct
     8881 ttcgataatt ccattctaac aaagctttgt aatgagagaa tcagtttctt gaaaaggttt
     8941 catgaacaat gtaaaggttg tatatggttt gtaaatttcg acgaattccc gttaaccttc
     9001 taaagctttt gcggtatgat agtagaaaaa aggaggctcc ctatgcagta ttatgatcag
     9061 catctccaca cgtatttttc ctttgattca gaggaaaaat ttgaaaacta tttagcctat
     9121 cagccggagt ttttcgtttc tacggatcac tttgatttaa acaatccatc taccggaaaa
     9181 gatgacattc cggattatcc agcctatata aacaaactgg actctttaaa agaaacctat
     9241 tccaccaaat ttctgcgagg tatcgagatt ggcgttgttc caggacaaga agagcggatc
     9301 gtggattacc tcgctcagca tccttatgat ttaaagctgg taagtattca tcaaaatggc
     9361 cgctttgatt atatggacga tgtggtttta accaaagatc ccattcaaac taccaaggaa
     9421 tattttgatc agatgttgca ggtcttgacg gattttccag aaggagatat tttgactcac
     9481 tttgattatg gattacgccg cttcaccttc acagcggaag aattcgcgga gcattttgag
     9541 gctcagctgc taacgatttt caaaaaggtt gttgaacgag aattggcgat ggagttaaat
     9601 gccaagagct tcttgaaata cggcaacgaa gacctttacc gttatgccgt tcctttgtat
     9661 caaagtgtcg gcggaaagct ctttacctta ggctcggatg ctcatgttgc cgaagattat
     9721 cagctgggct ttaaaaaaat gagtcagctg ttggcagact gtcaggtaga aaagttgcta
     9781 gtcattcaag gaaaagaacg ccgcctgacg ccgctgccga ttttgtgatt cctatataat
     9841 agaagaaagt ggtgaaaaaa atgagcaacc tgtgggacta caatcaagag gcacccatcc
     9901 attatttaat tgcgcgacat tgggatgcgt tgaaaattga ggcggtttgc cgatccttgt
     9961 tagcagccgt tccaaaacaa caactggaaa attttttggt ggctgacagc ttgcagcggg
    10021 aaaaagttca ggcttatttt gcagccttta aagatcagcc tttggaatat ttgcatgcgc
    10081 agtttcatct tttttatcaa gtagccgccc cagatgacta caacgattta cgaggacagc
    10141 tgcaattgac ctttcaagcg gatgaaaccg cctataccgt tttattggga atggctcgcc
    10201 ttggtgatca ggcgaaggtc gagtggcgga ttttcgatat ttgatggcag gatcaaggcg
    10261 tttcagttgc aaaccttcct ctctttcaat tagaaaaacc gcagcaggaa gaagtttgtg
    10321 tgctggtcac cgatgctggt gaaattcgtc tccgcttatt tcctgaccaa gctcccctgg
    10381 ctgtagccaa ctggcgctcc ctagctgagc aaggcgttta tgacaacaca cctttcggct
    10441 cgtgtcatta aggaatttgt tattcaaggt ggcgctttgg atggttctgg tgatgaagct
    10501 gtttccagct atgagggctt tttcgctgat gaagtgcatc gcggacttta tcattttaat
    10561 ggcgccttgg ccttagggga ccacggcccc cacaccaacg gcaaccagtt ttttattgtg
    10621 caaaacacaa aggcgcaggc tgacctgcta atgtaaagca agcatacttg gagcttggag
    10681 gactaccgga gctggatggt cgttatactg tttttggaca ggtataccaa gggttagaaa
    10741 tcgtagcagc gatcgcagct cagccgacaa acagtgaaga tgtaccgatt gagccagtgt
    10801 tcattcgaca aattaacttc gaaaaaacat cgtaaaaaga cactcactga cttcagcaaa
    10861 agctctattc ttgagctggc tgcagctagt gagtgtcttt catttatcca taagataaag
    10921 cttggattca ataaataaat aaatgttttg gttaaagctg tgaccagcaa atgaggaacc
    10981 gtgcgagcca gttgatctgc caatgcaaac atttctttag gtgaaggcgc tggcgtgtct
    11041 gccaacggat actccagtcc caatgcttgg taaccgtgca taccatagca gtggtacggt
    11101 agtaattcta acggtacgaa ccgatcaaga tctgccacaa aacaagcaat cgtcagcagc
    11161 tcggtcaggt tcatattgat acctggaacc acaggaactc gaattcgcac ttcgcagtta
    11221 ggatactcaa ttaaaaagcg acgaatattc ttcaaaattt gttggttatc taagccggtc
    11281 cattgtttgt ggagcttgct ggtaaacatt tttacatcca caaacaatgt atccacatag
    11341 ggagctacct tttggatttc attataagca ccacaaaagc tggtttccag tacggtattg
    11401 attccaatgt atttcgattt ttgcaaaatt tctttagcaa aatccgcctg aaccagtgcc
    11461 tcgcctccgc taatggtcac tccgccatcc gagtgaaagt aaaaaacagc atccttctca
    11521 atctcatcca ttacctcttc agcggtcatg gtctggccaa atcctttttc aatctgcttg
    11581 ctttgtgatt cgggaatcga acaccaggca cagcgtaatg ggcagccctt taaataaaca
    11641 acggtccgta aaccagcacc atcatgtaga gaaccctgtt ctattcttaa tactgttcct
    11701 tccatataga tttttgaatt acacactagt ttaaactaaa acacacaagg aaaaacttac
    11761 catttaatca taaaattagc tcatataaat atactaggat gtaaaataga ctggtcaaaa
    11821 tctggtcaaa aaagaggact aatacaggta gtcctctttt ttgttatttt aatattctaa
    11881 atcatttttt attggtttat tcccttattc gatgtagact caaatcacta tatcactaga
    11941 tatcaaacgg cgtcatacgg aatcaaaaac atcaaatttg acctgttaaa tggtcattga
    12001 ataacattca aaatcaataa aacggagtca gacacggagt caaaaacgct gagagtaaaa
    12061 aatatataca gccttgtagg taaagtacca cagggctgta tattttttat ttcagattac
    12121 cttaaatgat tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc
    12181 tcctattttc tcaataaact atttttggat acatggtatc tgaaacagct aaaaccttgt
    12241 cattacagca aaaaatcacg ttat
//
LOCUS       DACEKH010000080        12246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEKH010000080 DACEKH010000000
VERSION     DACEKH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.083"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW662_002082"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW662_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ0866898.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW662_002083"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW662_002083"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ0866899.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IW662_002084"
     CDS             complement(2169..2906)
                     /locus_tag="IW662_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0866900.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IW662_002085"
     CDS             complement(2997..3524)
                     /locus_tag="IW662_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ0866901.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IW662_002086"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IW662_002086"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ0866902.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IW662_002087"
     CDS             complement(4401..4886)
                     /locus_tag="IW662_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ0866903.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IW662_002088"
     CDS             complement(5039..5236)
                     /locus_tag="IW662_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866904.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW662_002089"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW662_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ0866905.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW662_002090"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW662_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ0866906.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IW662_002091"
     CDS             9211..9423
                     /locus_tag="IW662_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ0866907.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IW662_002092"
     CDS             9425..11104
                     /locus_tag="IW662_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0866908.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IW662_002093"
     CDS             complement(11358..11558)
                     /locus_tag="IW662_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0866909.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="IW662_002094"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="IW662_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         3575 a         2636 c         2117 g         3918 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgacatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaat
//
LOCUS       DACEKH010000081        12094 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEKH010000081 DACEKH010000000
VERSION     DACEKH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.084"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            598..1926
                     /locus_tag="IW662_002095"
     CDS             598..1926
                     /locus_tag="IW662_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0866910.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2128..2961
                     /locus_tag="IW662_002096"
     CDS             2128..2961
                     /locus_tag="IW662_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ0866911.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2977..3714
                     /locus_tag="IW662_002097"
     CDS             2977..3714
                     /locus_tag="IW662_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ0866912.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3869..4837)
                     /locus_tag="IW662_002098"
     CDS             complement(3869..4837)
                     /locus_tag="IW662_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ0866913.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5069..5902
                     /locus_tag="IW662_002099"
     CDS             5069..5902
                     /locus_tag="IW662_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ0866914.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5936..6775
                     /locus_tag="IW662_002100"
     CDS             5936..6775
                     /locus_tag="IW662_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0866915.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6801..7361
                     /locus_tag="IW662_002101"
     CDS             6801..7361
                     /locus_tag="IW662_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0866916.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7560..9281
                     /locus_tag="IW662_002102"
     CDS             7560..9281
                     /locus_tag="IW662_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ0866917.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9446..10588
                     /locus_tag="IW662_002103"
     CDS             9446..10588
                     /locus_tag="IW662_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0866918.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10604..11815
                     /gene="thiI"
                     /locus_tag="IW662_002104"
     CDS             10604..11815
                     /gene="thiI"
                     /locus_tag="IW662_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ0866919.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4129 a         2030 c         2511 g         3424 t
ORIGIN      
        1 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
       61 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
      121 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
      181 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
      241 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
      301 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
      361 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
      421 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
      481 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
      541 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
      601 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
      661 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
      721 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
      781 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
      841 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
      901 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
      961 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     1021 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     1081 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     1141 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     1201 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     1261 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     1321 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     1381 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     1441 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     1501 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     1561 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     1621 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     1681 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     1741 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     1801 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     1861 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     1921 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     1981 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     2041 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     2101 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     2161 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     2221 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     2281 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     2341 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     2401 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     2461 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     2521 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     2581 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     2641 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     2701 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     2761 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     2821 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     2881 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     2941 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     3001 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     3061 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     3121 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     3181 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     3241 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     3301 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     3361 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     3421 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     3481 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     3541 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     3601 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     3661 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     3721 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     3781 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     3841 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     3901 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     3961 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     4021 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     4081 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     4141 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     4201 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     4261 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     4321 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     4381 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     4441 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     4501 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     4561 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     4621 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     4681 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     4741 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     4801 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     4861 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     4921 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     4981 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     5041 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     5101 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     5161 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     5221 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     5281 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     5341 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     5401 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     5461 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     5521 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     5581 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     5641 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     5701 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     5761 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     5821 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     5881 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     5941 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     6001 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     6061 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     6121 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     6181 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     6241 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     6301 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     6361 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     6421 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     6481 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     6541 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     6601 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     6661 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     6721 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     6781 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     6841 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     6901 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     6961 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     7021 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     7081 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     7141 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     7201 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     7261 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     7321 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     7381 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     7441 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     7501 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     7561 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     7621 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     7681 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     7741 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     7801 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     7861 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     7921 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     7981 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     8041 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     8101 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     8161 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     8221 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
     8281 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
     8341 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
     8401 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
     8461 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
     8521 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
     8581 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
     8641 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
     8701 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
     8761 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
     8821 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
     8881 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
     8941 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
     9001 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
     9061 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
     9121 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
     9181 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
     9241 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
     9301 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
     9361 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
     9421 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
     9481 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
     9541 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
     9601 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
     9661 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
     9721 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
     9781 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
     9841 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
     9901 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
     9961 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    10021 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    10081 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    10141 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    10201 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    10261 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    10321 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    10381 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    10441 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    10501 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    10561 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    10621 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    10681 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    10741 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    10801 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    10861 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    10921 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    10981 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    11041 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    11101 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    11161 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    11221 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    11281 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    11341 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    11401 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    11461 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    11521 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    11581 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    11641 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    11701 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    11761 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    11821 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    11881 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    11941 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    12001 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    12061 aagtgggcag cttcaagaaa taagaaggaa ttta
//
LOCUS       DACEKH010000082        12033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEKH010000082 DACEKH010000000
VERSION     DACEKH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.085"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            960..1928
                     /locus_tag="IW662_002105"
     CDS             960..1928
                     /locus_tag="IW662_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0866920.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1945..2469
                     /locus_tag="IW662_002106"
     CDS             1945..2469
                     /locus_tag="IW662_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0866921.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2845..3126
                     /locus_tag="IW662_002107"
     CDS             2845..3126
                     /locus_tag="IW662_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866922.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3128..3544
                     /locus_tag="IW662_002108"
     CDS             3128..3544
                     /locus_tag="IW662_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866923.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            4176..5330
                     /locus_tag="IW662_002109"
     CDS             4176..5330
                     /locus_tag="IW662_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ0866924.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            5324..5746
                     /locus_tag="IW662_002110"
     CDS             5324..5746
                     /locus_tag="IW662_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866925.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            6057..6437
                     /locus_tag="IW662_002111"
     CDS             6057..6437
                     /locus_tag="IW662_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0866926.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            6419..7333
                     /locus_tag="IW662_002112"
     CDS             6419..7333
                     /locus_tag="IW662_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0866927.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            7293..7571
                     /locus_tag="IW662_002113"
     CDS             7293..7571
                     /locus_tag="IW662_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866928.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            7578..8267
                     /locus_tag="IW662_002114"
     CDS             7578..8267
                     /locus_tag="IW662_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866929.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(8388..8474)
                     /locus_tag="IW662_002115"
     CDS             complement(8388..8474)
                     /locus_tag="IW662_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0866930.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            9111..10055
                     /locus_tag="IW662_002116"
     CDS             9111..10055
                     /locus_tag="IW662_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ0866931.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            10076..10609
                     /locus_tag="IW662_002117"
     CDS             10076..10609
                     /locus_tag="IW662_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0866932.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            11249..11473
                     /locus_tag="IW662_002118"
     CDS             11249..11473
                     /locus_tag="IW662_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ0866933.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            11493..11780
                     /locus_tag="IW662_002119"
     CDS             11493..11780
                     /locus_tag="IW662_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0866934.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
BASE COUNT         4582 a         2042 c         2084 g         3325 t
ORIGIN      
        1 cactttagtg agttttgtag aaagtacctt taaaggtact ttcacattac gaacgatacg
       61 tccgtaggac gtactgaaag ggtgggattt atgagcgaac taatgcaaaa tttggctagc
      121 aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat gaacactttt tcaagtgagc
      181 gaatccgcca tgacaaagct gaagcagttt atttcagctt tgtcatggcg tgtgcctaga
      241 cttttaaaaa agtcggcaca cgagcaaata tctttatcaa gctctaattt gcgttttaag
      301 acgtttgaaa cgtctaagta tataaatata taagagtagg tttaagagtg cttaaatcga
      361 attttagagg gcattcagac aactttaggg ttgttagggc ttgccctgac cgcctgtaag
      421 gcgctttaag aacaagataa gggatttata ctaagactgt cttaaaacgt ctcaaacgaa
      481 ggctcatagc ttctcaaaga gttccttatg aacattttta gcaaaggagc cggaagcgac
      541 tgcgcagtat aatgtgagag cagagcgatc acaccttttt aagcgactgt agggagtaag
      601 attcccagtt gagaaaaatg cgcgaatctg tttggagaaa aaagaagtgg ttttttaggc
      661 gggaaatttt gtccccctct tttataatct ctttttaaac ctctttttaa acctctttac
      721 cgtcggctat atccttactc tcccaaggga atgacgcttt tatctacatc gttttgtctg
      781 tttatctaca tcgttttgtc tgtttatcta catcgttttg tctgtttatc tacatcgttt
      841 tgtctgtttg tacattgaat tagtacatcg acgatgttaa tataagatgt agataaacag
      901 gcattttgta tttcataacg tgtatctctt gtgcgagtaa gaaaggagct attttaaata
      961 tgtctaatga aatcgtaaag tatcatcatg aattaaatac aattccgttg cgaaaattta
     1021 caccagtcga aatgaatttg ttcttttcta tcgtatcaag aatgcgtgat gtaggtgata
     1081 aaaaagtaca atttacattc gagcaattaa aagatttaag caattataaa gcaactgcaa
     1141 atgttcgatt catagatgat ttagaaacaa cgtatgacaa attaatggat ttacgttttg
     1201 gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt tttatttaat caatttaaaa
     1261 tcgacggaaa atctgatatt ccttttgctg aaatacaagt tcatgaaaaa gccttaccct
     1321 tacttaataa tcttgaagaa tgggtgcgct attctctcca acaattcaat gaattagaaa
     1381 gcagttattc aaaaacaatg tttcgattac taaaacaata cagaaccaaa ggtttcgctt
     1441 atttttcaaa agaagacttt catgaattac ttgatattcc taaaagttac aaccaaggta
     1501 cgattgatca acgtgtgttc aatccaatac gacaagaact aaccgcaatc tttaaaggtt
     1561 taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc tgtcattggc tatcaattta
     1621 catttaaacc tgagataaaa aaatcagacg actttaacaa acatgcccca acaaaagaag
     1681 aacaaaacaa agcattagaa ctagctaacc aacaagttca atctgaaaaa gcacctactt
     1741 cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga acttttacaa cagttagaaa
     1801 agttagaaag agaacaaaat acccaaaata aagaaaataa tgcacaaaaa tcagagataa
     1861 attccgatat tccttcagaa ctaaaaaaag acttattaga caatcttcaa aacttattca
     1921 aagattgagg ggtttttaga ttttatgaca gaaaaaacaa tcaaagaact tgctgaagaa
     1981 ttaggagtta gtaaaacagc aattaacaaa aaagtttctg acgaaaatcg aaaactttgg
     2041 ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg gacaaaaggc tataaaatca
     2101 atgtttttta acgaaaacga aaactcaaat caacaactag ttggcgaaaa aacgaaaacc
     2161 gaaaaccaca aaaacgccaa ccaagttccc gaattagaag tcctttctct cctagaacaa
     2221 cagttaaatc aaaaagatat gcaactacaa gaaaaagacg agcaaataaa acaaatgcaa
     2281 aaattactcg accaccaaca acaactcacc ctgcaagcca accaacaaat cgagaaactg
     2341 caggaacaac tacagttgac ttatacggaa gaatcttcgg aaaataaatc aacgactctt
     2401 tctgaagaaa cagaatcgat agaaaaacaa tcaaaggcgg agaaaaaatg gtggcaattc
     2461 tggcaataaa accagcaatt taaattaata aaaattgctg gttttattct tctttttgct
     2521 tttgcgagca gagcaacatg gacccatgtt gcgatatttt ggtggcgccc caatccccgt
     2581 tttttcgttg aatcttactc ggactgcact cgttttacag ctgatttttc tgaattttga
     2641 aagagcataa ggaaacgtgg tatcactttc ttacgaaagg tgctagatag gctgtgccta
     2701 tcacgtttct cagaaaaaaa tttgctatca cttttttgcc attgtggcta ggctatgtat
     2761 agtgttttgg cgaaaaatag ctatacaaat actatatttg tatggtatac ataaattata
     2821 cgctgatacg aaaggaaaat ttccatggac attttagttc gaaatatcaa tcccaaaatt
     2881 attgcaagaa tagataaacg ttgtcaggaa ctcacaacca aaacgggcaa aaaatggagt
     2941 cgaaataaat atctaaaagt tctaatcgaa agtgattttg accgtcaact tatgaattac
     3001 aaaaaagatc aattcgattt attactcgaa aaatttattg ccattcaaac agaaaatacg
     3061 aacatactga atgactatgt tcacacaaac aaccaactca ttgaaacact tatcggagga
     3121 atataacatg agaaaaactt ctgttcaaaa acacgtaacc ctttatccca gtcacgttga
     3181 cgttttagat tccatcatcg aaaaaaatcc agacgtcaac aattattcag aagccattcg
     3241 ttttttatgt atgagttatg aacctgatac caatacaacc acaaccagta accaagcaaa
     3301 actcaatgcc atgggaaaag aaatttctat catgacagaa atcatggcag aatttgctga
     3361 tttatatcta aaagatactg gtgttcttat cggtacaaat tctaaagtgt atcaagaagt
     3421 gaaagaaaaa attgaaaatc aactcaatcc ttcccgacga aatcaaccca aaaatcgtca
     3481 aaaagaaacc aatcaaacct ttacttctgg aaattttgac ccaacaaact ttctaaaaag
     3541 ataaaaaaaa gaaaccgatc cctaaaaaaa attcctcgat ttctttttag ggatcagtct
     3601 tccagcaaga tttgcaagtt ggtaccaact tgcgaaaaaa aagaaaaatc cgcttatgca
     3661 acttcttctt tttgtatttt gaggcgctac cccaatcccc aagtatcggt tatttaatag
     3721 cgcggaaatc caaagaatag gacgtaccct gaaacaaaaa aagactgctc tcacgagcag
     3781 cccaaaatga gattatccta ttcatcaaca taaaaatatt acaaagtgaa gaactagaga
     3841 aagacaaaaa aagcatatca aatcctctca aaaataacaa gtctttaaaa aatgacagat
     3901 aaaaatgcac aaagaactaa aaaagagaaa tcgcgtaaaa tcaagcatta gaagctagta
     3961 actagctaga ccaattaaaa atcacaaagt gagcgtctct atccattggt agaggggcta
     4021 cttggcatag tgtacattat gttaactagc tcgataaata acgattatgt tcccaaaaca
     4081 atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta attatataat
     4141 tataggtgta taccacttaa atttaacaca aagacgtgat tattttgaaa cccaaaaaga
     4201 aaatttatct aggtctcctc ttttccttga tgattggaat ttttagtata ggaattacaa
     4261 atacctatgc ttcagaaaat tctaatttca atatcaactc ccaaccaatt gaagaacaag
     4321 caattaattt tgcgaaaact gattttgaac actccataac ttcaattctt ttaatggaaa
     4381 ataacaccga aactgatatt aactatactc ttggttcgcc atttaaaatg ctcgatggag
     4441 aaaatacggc aaatagttct attttctttc cagtattttc agacgctgat aatacgatta
     4501 aatatattta taccgtcaaa aaaaatacaa atggagaata ttcaggaagt atctcacaat
     4561 ttttagcaaa agaacttcaa aatctagtag atagtaatat aacagataca accttatttt
     4621 ttcaagataa tagcatttat tattccgata atggacaaat taaactactt tggaaatcac
     4681 ctttaagtac agaaaaaaat ggtgtttctt cctttacaga aaaaaatgat cttatatccg
     4741 taaatataaa aaataaaatt aaaaatttct ctaaaaaaca aaattttcct gcggttaaac
     4801 gtgtaaaaag tgtctcctcc ggagataact ttattatgat taattggcgt atagcagaaa
     4861 cccaaacaag ttttccgtgg tgtgctgctt acgtagaagc tgctatctta tgtaacaaaa
     4921 cagatgtacg tgctacttca gctagagcca ttatgcatta tacctatcct aatttaaaca
     4981 acagtcaaat tgaatcaaaa agtcttaatc aagatcaaat aataaaatat gcaaattact
     5041 gtggtgttta tcctaaaaaa gtagcacgaa tactaaacct ttcagaggtc caaacacaac
     5101 taagaaaagg aaatgccatt tatatgggaa ctgctggtta tggaaattat agtaacagcc
     5161 gtcatgcact tgcattattt gggtgggtca atcaaggtgg ttatcaaact tactatgtgt
     5221 ggaatccttg gtataactac ccaactgtaa taagtgcaaa tgcaggacca gttacaattc
     5281 ctgtacctgg aggtggtttt acttgggaca ataccattac aaattggtaa ataaacccat
     5341 tgctttagtt atgggtctag tgttagttct tttcttaact agttgttcaa aaaaacaaga
     5401 aatacaaaag actgatctga ttccttttct aggaacttgg caaactaaag atcattcaga
     5461 acgtctagta atcactaatt taaatacttc aaaaaatata gaacaatata ccatcttagt
     5521 aaaagagaac aatcataaaa ttcaaatcat tgataaaaat ttaaatataa atgaacaagt
     5581 tttcatttac aaagcaacaa ataatttact aacactgcgc tctaataaaa aacaattaga
     5641 ttacactttt agtttggatt ctccaaaaaa gctcgtattt attttgacaa cgaatgatgg
     5701 gacagaaggg acagctaaac caattgaatt ttttaaagct gattgatgaa tagttgtttg
     5761 atattacaaa acaataattt caccgaaggt gaaacaagcg aaactttaca aaaagtggct
     5821 agcatttgct agcctttttt gtattgtgga gcgtggtttt tgagggggat tcaaggggga
     5881 gcttgcgccc ctttgacaag cggttatttg caccaaatac gtaggtattt ggctagcaaa
     5941 tatagagctt gccaagccct ttagtgagct ttagtgagct ttacttattg taccttttta
     6001 ggtacaatgg tattgcgaac gatacgtccg taggacgtac tgaaagggtg ggacttatga
     6061 gcgaacaaaa tcaatatttg gctagcaatc attctaaaaa atatacctat cgatccgagc
     6121 caaaacaaat cagttttcga gtgagcgaat ccgaatttgc aaagctgaag cagtcagctg
     6181 aagctttgca aatgagtgtg ccggctttcg tgaaagccaa ggcacaaaac gcgcgtctgg
     6241 tgacaccgaa agtggcgcca gacattgctc aggcaatcgc gcgcgattta gccaaagctg
     6301 gcgggaatat caatcaaatt gccaagtggt gcaacacaca ccaacacgac gttgatcctg
     6361 gcgacgccca acgcttatcc gaaaacctaa aaatcatgca aaaggagcta caaaagatat
     6421 ggcaacagtt aaagtaagcc gaaccacttc ttgtagccga gcgatcaatt acgccgaacc
     6481 acgagcaacc gtcaaaacag ggataaactg tgacattgat tatgccaaaa gcgaaatgaa
     6541 acaaattcgc atgctgtatg gaaaagacga tcgtgtacaa gcacacttat tgatccagtc
     6601 ttttcgacca ggagaaatta ccgctgaaaa agccaatcaa ctaggggcag agtatgcgaa
     6661 aaaaatagcc cctgaccacc aaatcgcgat ttacacacat acagacaaag accatattca
     6721 taaccacatt gtcattaact ccgtcaatct ggaaactggg aacaaatttc aagcccatgg
     6781 tcaagcattt ttaaacaaat gttatgacct aaatgacgag atttgtcttg aacatgactt
     6841 aacaatcact gaacgaggga agaaacccga aaaacgaacc atgtctgaaa tcaaattaaa
     6901 agaaaaaaat gaacctgttt ggaaagacga aattcggttt gcgattgatc aaacaatgaa
     6961 aaacaaaaaa actcgaacgt ataaccaatt ttgtgattct cttaaaacat tcggcattca
     7021 ttgcttgaat cgtgggaaaa attttactta cgaattgatc cacaaaaaga aaaaagtacg
     7081 atccaacaaa ctaggaaagg attacgaaaa ggagacgatt ttacatgagc tggacagacg
     7141 agaacgagcc tacaatcaac aacgaaccaa cgagctacga agagctcgag ctagcattcg
     7201 cgaaactgca aaagaagtac gaagagaaca acgaactctt acgactgaac aaccggaaat
     7261 tagaccgact cgaagaaacc atgaccaaca cgttgaaaga ggagcagact accaacaaga
     7321 attatcgttc taaaacgtta caagacatag aaaatgaaat aaagaaacaa cgtgcttttc
     7381 ttgcacctta taccaaagaa agaatacaaa ctacaattga ttcacaagtc aaaaaattcc
     7441 accatgcgct attaaaccag cgaaataaag aacagaaaaa agtaattatg cagcgatcaa
     7501 tggatattct tgccccctta tgttgcttaa tcatgacact gttagccttt tggctggtat
     7561 ggggagaata aatagaaatg gcattctatt taccctatct actcattttt gccagtattt
     7621 taggaattat ttggcttagc tataaggctt atcaaacaag atattcatta aaaggctcta
     7681 aaattcgatt taagaggttt ttattgccag gggttatatt tacattatca atcgttttaa
     7741 gtggtttatt cggcgtttta gagggaaata aacgtatttc aagaagtatt ttattagggg
     7801 atatcactca aaagtatgaa gcagcatgtg ataaaaagaa aaaggagcaa gtgttaacac
     7861 aaaaaatgga agagtttacc acttgttatg aggaaatgaa cgatattttt gtgaatcaag
     7921 aaaaacgttt gacggataaa aacatggaaa aactcactcg tctttatcaa aacttgccag
     7981 aaaaacagca aaaagaggtt caagacaact atgagcgaac caaaaaagac gtgcaatatt
     8041 ttaaagatac gcagacagaa gaagcttgtt ataatttatt cagtgataca atcccttttt
     8101 caactagtga acaggaacga aaagaacggc aacaatcagt tacctacgaa cgttataaag
     8161 cactgcttca gcaagcaacg aacatacaaa atccaacgaa aaaagaaacg gcattaaatt
     8221 atctaaaaag tgtcaaggaa tggttagatc agcaacagca aaattgaatt tgatcggcaa
     8281 tcatgttaaa ctaaacatgc aaatgatcaa acattagata cacgaaagcc ccagagaggt
     8341 cggattctct ggggcttttt ttcaggttga tgtcgccttt attgatttta cttcttgttt
     8401 cggttattca accaataact gaataacgag acacaaactt ctataagaag ggggcaaatg
     8461 aacaattcaa acattagata cacctccttc cagtcgcaag actgtcggat aggcgacaag
     8521 ttattatatc ttaaaaaatt aaacttgtgt ggtgttttta agtatcgctt attttagggt
     8581 tgttagggct tgccctgacc gcctgtaagg cgctttaaag ctagataagg atttttatcc
     8641 aagactgtct taaaacggct tagataagca ttcagaaccg ctcaaaggcc tctttaccaa
     8701 catgttttta gcaaaggagc ctttaggcga ctgcgcagta caatgtgaga gcgtagcgat
     8761 cacacctttt tggcgaccgt aggaagcaag gttttggatt ggcagttttg aaaaatgcgc
     8821 gaacctgttt aaagaaaaaa gaagtggttt ttaggcggga aaattagccc ccctcttttt
     8881 atatctcttt ttaaacctct ttttaaacct ctttaccgta agctgtgtcc ttactctacc
     8941 aatggataca gaatgttatg aaggacattt tgtctgttta tgaaggacat tttgtctgtt
     9001 tatgaaggac attttgtctg tttatgaagg acattttgtc tgtttgaacg tcaaatatga
     9061 atgtcttgac atttaaaaaa tgtcattcat aattggattg aggtgattat atggctaatg
     9121 agattgtaaa ataccaccat gaattaaata caattccttt aagaaaattt acaccggttg
     9181 aaatgaattt gtttttttcc atcgtgtcta gaatgcgtga aaaaggggat aagacagttc
     9241 gttttacgtt tgaccaacta aaagatttaa gtaactacaa agcaactgca aatgtacgat
     9301 ttgtagaaga tttaaaagca acttatgaaa aaatcttatc tttacgcttt ggtcgtagaa
     9361 gtgagtctgg gctttcttac gatatgtttg taatgtttac agaatttaaa atcaacgggg
     9421 acgtagaaaa tccttacgta gatatacgaa tttacgaaaa agcactgcct ttgttaaata
     9481 atttagacga gtgggtacgc tattctttac aacaattcaa cgagctacaa agtggctatg
     9541 caaagactat gtttcgtctg ttaaagcaat acagaaccaa aggtttcgca tatttctcca
     9601 aagaagactt tcttgaatta ttagatattc ctaaaagtta taagcaacct gatatagata
     9661 aacgagttat aaaacctata cgtcaagaac taactgcaat cttcaaagga ctaacaatca
     9721 aaaagaaata cggaaaagga cggggaaaac ctgttattgg ctatcaattt acgttcaaac
     9781 ctgagatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa gaccaaaata
     9841 aagcattggc tttagctgat aaacaaaaag aatacgaaga acccgctaca acaattaacg
     9901 aaaaaatcaa acagaagaaa cgagaacttg aactcctcca acaaatcaaa caactagaaa
     9961 aagagaaaaa agctcttgaa aaacaaacag agtccgcact ttcggaaaaa gagaaaaaaa
    10021 gtttattatc tgatttagat aatttattcc agtagcaacc taggaggcaa ccagtatgca
    10081 accacaagaa acctttacaa taaaggaatt gtcagatctt tttaagatga gtaggcaagc
    10141 aatatcgaaa catatccaaa aattagattc ttccatgata gctaaaaatg agcgtggtta
    10201 taaggttgta ttgcgttctg gagtattgca acttgctcgc aacttagaca atcaaaggtt
    10261 gctagataag ttgcataaac atgaacaaaa caacgataaa aatgcttata taccaaacaa
    10321 aaacgtggtt gcaaacaagt tgctagatca gttgcgtgaa gaaaatgaat ttttacgaga
    10381 acaacttgcc ctcaaagagc aaaataattc tcatatacaa aaactactcg accagcaaca
    10441 acaactcacc ctgcaagcca accaacaaat cgaaaaactt caggagcaac tgcagttgac
    10501 ttatacggaa gagtcttcag agaatagttc aacgactctt tctgaagcaa cagaaacaat
    10561 ggacgaacaa acaaagaatt ggaaaaaatg gtggcagcta tggaaatgaa gaaggaaata
    10621 cttatgacaa aaaaacactt gctattcggt tctatcctac taggaatatt aagctttagc
    10681 ccaatgttat tatcagtggc caacaaagga aataaaaaag gggaactaaa actcaatcct
    10741 aattactaca acagtcaaaa tgaggaagta tttgtagaaa aacttgataa ataagaaaag
    10801 actgctcgta cgagcagtct ttctgaaaga gattatccta ttattaacct tagaaaagaa
    10861 taataaatga atatgagata tgtaaaaagc ataacaaaca caaacaaaaa taacaagtat
    10921 ttaaaaaatg accgatcaat attcacaaag aattagaaag aaacaaccgc agaaaaccaa
    10981 gccttaaaaa gagcttttaa cctagaccaa taaaaattca caaaatcagc gcctggatcc
    11041 cttggtacag gcgttacttg gtagtataca cattatgtct actagctgtt taaataactg
    11101 atcttataat tttatttaaa cgattatgaa ttcaaaacta ctttttatga cgttaatcat
    11161 tgttatataa taaaatgtta tataataaaa ttactaagat tatttaatat aacaagcaat
    11221 tttaaaaaaa cgaaaaggaa aaacaatcat gaaaaagaaa gtattaaaac attgtgttat
    11281 tctaggaata ttaggaactt gtctagctgg catcggtaca ggaataaaag ttgatgcagc
    11341 tacttactat ggaaatggtc tttattgtaa caaagaaaaa tgttgggtag attggaatca
    11401 agctaaagga gaaattggaa aaattattgt taatggttgg gttaatcatg gtccatgggc
    11461 acctagaagg tagtggtagg gaggttatta taatggattt tactaaagaa gaaaaacttt
    11521 taaatgcaat tagtaaagta tacaatgaag caactataga tgactatcct gacttaaaag
    11581 aaaagctctt tctttattct aaagaaatca gtgagggaaa aagtgttggt gaagttagta
    11641 tgaaattaag tagttttctt ggaagatata ttttaaaaca taaatttgga ttacctaaat
    11701 ctttaataga attacaagaa attgttagta aggaatctca agtatataga ggatgggctt
    11761 ctattggtat ttggagttaa ttctttggaa taaaatgaaa atgggactga agaatcaatt
    11821 ctgattcatc agtcccataa attatagagc cgaaaaatta tcatatttat agatttatta
    11881 atagtaaatg gcataagaat ctttgaacaa cgaaaaaaat gtagctctac ctaattattg
    11941 atttgcaaca atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
    12001 tgctagtctt ttttgtattg tggagagtgg ggg
//
LOCUS       DACEKH010000083        11995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEKH010000083 DACEKH010000000
VERSION     DACEKH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.086"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            106..1524
                     /locus_tag="IW662_002120"
     CDS             106..1524
                     /locus_tag="IW662_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ0866935.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            1595..3064
                     /locus_tag="IW662_002121"
     CDS             1595..3064
                     /locus_tag="IW662_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0866936.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            3074..3541
                     /locus_tag="IW662_002122"
     CDS             3074..3541
                     /locus_tag="IW662_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0866937.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            3577..4449
                     /locus_tag="IW662_002123"
     CDS             3577..4449
                     /locus_tag="IW662_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0866938.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            4542..7064
                     /locus_tag="IW662_002124"
     CDS             4542..7064
                     /locus_tag="IW662_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0866939.1"
                     /translation="MKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQLFSD
                     GKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLPLIIK
                     GNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKKGAFT
                     GAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEYANVR
                     LILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITIDSKIF
                     CSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWIDPEI
                     SIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESDEFIA
                     LRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFRMKND
                     KQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITASAIAR
                     ETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKI
                     ESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKN
                     VIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLST
                     TEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLRFL
                     DSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEGA
                     QLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            7220..7993
                     /locus_tag="IW662_002125"
     CDS             7220..7993
                     /locus_tag="IW662_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0866940.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            7986..8777
                     /locus_tag="IW662_002126"
     CDS             7986..8777
                     /locus_tag="IW662_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0866941.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            8787..9278
                     /locus_tag="IW662_002127"
     CDS             8787..9278
                     /locus_tag="IW662_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0866942.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            9296..9706
                     /locus_tag="IW662_002128"
     CDS             9296..9706
                     /locus_tag="IW662_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0866943.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            9710..11110
                     /locus_tag="IW662_002129"
     CDS             9710..11110
                     /locus_tag="IW662_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ0866944.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            11133..11507
                     /locus_tag="IW662_002130"
     CDS             11133..11507
                     /locus_tag="IW662_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0866945.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         4127 a         1624 c         2404 g         3840 t
ORIGIN      
        1 attccttcgt cgatttaaaa atatttttat gaaacgggtt gacatatgaa acgggttgac
       61 atatattagt tgtgaagata aaaacctttt cactggagga agaaaatgga gaaattatta
      121 attaaaagca ataatcatca actttactac caacctaagg atgtttgggt cggagatgtt
      181 atgccttacg ggaaagaagg aaaattcttt atttatcatc aacgtgatac tagaaatcca
      241 gttccttttg gagaaccgtt tggctgggct ttagcaacta cgaaagattt tgttcatttt
      301 gaagactttg gggaatctct tttaagaggg gatgacaatg agagagacca gtttatctac
      361 gctggttctg tatttgaggc aaatggaaag gttcacgcat tttatacagg gtataatcgt
      421 gaattcttag cgcagggaaa gacatctcaa gtgttgcttc atgctgagag tgatgatttt
      481 gttcattggg aaaaatcaga aacagcattg gagttagaac cacaagaagg gtatgatatt
      541 cgaaactgga gagatccatt tgtcgtttgg gatgatgaaa aagaagaata tttgctaatt
      601 ttaggggcgc gaaagggtca ggataaacat ctacaaaccg gacgtttagt ggaatttaca
      661 tcaaaagatt taactaattg ggaattcaaa ggtgattttt gggctccggg attgtatacc
      721 atgtttgaaa tgccagaact tttcaagatt ggtgactggt ggtatcttgt ctattcagaa
      781 tactcagatg gaaataaaat actctatcga atgagtcaaa gtttggaagg tccttggatt
      841 aagcctaaag atgaagcttt tgatggacgg gcttattatg caggtagaac agcttatgat
      901 ggaaaacgtc gagtcctatt tggatgggta ccaacaaaag atcaagaaaa tgataaaaac
      961 aattatttat ggggaggtac tttggttcct catgaagtct atcaaaaaga ggatttttca
     1021 cttggggtaa aacccataga cgaagtgtgg aatgcattca taaatttaca tgatattgcc
     1081 gatgtagaga tagaaacagc agacacaaga gaagaagtaa cgcttgtcaa aaatagtgga
     1141 actttgttta atttagaaac aacaatttca tttgatgaga atcttagtga cttttcactg
     1201 agattatata aaaaccttga gacagatgaa tcttacgaat atcgcttcaa tttagatgag
     1261 gggcgtctta catttgataa aaacccttgt tacccttggt ttcaatatat ggataaagga
     1321 cttattagac ctatatcttt aaagcctaac caatcttata aactaaaagt gattgttgat
     1381 gacaatataa tgacacttta tattgatgga atcgctctta acgctcgtgt acatggttgt
     1441 tttggtcaag caattactat tacagcaaca aatggatcaa tgactgcaat aaataccaag
     1501 ttttcaaatg aaataaagaa ataataaacg tcaatcatgt ggcaggtctg aaaatcaaac
     1561 atgacctgtc acatttaaaa gggaggataa atgaatggac tacaaaaaaa ttgccgaaga
     1621 tgtattggaa aacgttggag gaaaagagaa tatctcaagc gctacacact gcattactcg
     1681 tttacgttta attcttaaag acaaaagcaa agcgaatatg gaaatgcttg aggaaataga
     1741 aggtgcaaaa ggtgttcttt tcaatagtgg acagttacaa gtaattttcg gaacaggtat
     1801 aattgaaaag gtttacaaag agttcgttga attgacagga gctaaggaag tatccgtttc
     1861 tgaaatgaaa gacgaaggag tcaataattc aggaaataga cttcaacgag cttttaaagt
     1921 tttctcggat atctttattc cgatcattcc agcttttatt ggggcagcaa tggttttagg
     1981 aattcgttcg ttgattacca ctcctggaat gtttggaatg gatggctcat tcgctgatca
     2041 caatgtattc ctaaatgatt tatccaaatt tctgggtatt attgcaacca catttgatta
     2101 tttaccagtt ttagttatgt attctgcaac aaaacgtttt ggcggaaacc ctattttagg
     2161 attacttgta ggatttgtta tgattcatcc aggactagcc aatcggaatg atttcgtcat
     2221 gggaactata aagcctgatt attggcattt atttggatta agcattccag cagttgcgtt
     2281 tcaagggggt gtttttccag caattttgac ttcttggttt ttagcaaaag ttgagaaaat
     2341 tacaaataaa tattctcctc aagtacttgc ttacatttta gtcccaactg tgacaattgt
     2401 tcttgccaac tttgcactat ttttagtgtt tggtccattg gggaatttta tcggtaatgt
     2461 tttaggtgga atcattgatt tcctatatat gaagatgggt gcctttggtg cttttgtttt
     2521 tgcaggcgcg ttacaaccat tagttgtaac aggaacacaa catgctattc aaggtattga
     2581 ggcaaactta gtcgcaacaa ctggattcaa ttatatccaa cctatttggt cagtttcaat
     2641 tattgcccaa ggtggcggtt gtattggtat gtatttactc tttaagaaaa aatctaaaga
     2701 tagagatatt gcgatttcaa gctttattcc aacactggta ggtattactg aaccggctat
     2761 ctttgctgtt aatttgaaat attcaatcgt tccttttgtt tgctcgttta ttggtgctgg
     2821 ttttggtgga gcatttatga aaatttttga tgtaaaagcc attggacaag gattgactgt
     2881 attgccaggt ctgactattg ctaatccagt tcttccttat atatttggta atctcatagc
     2941 attcatttta ccaatcattt ttattctcgc ttatgataaa tataaagtgt ttatccctga
     3001 agctaaacac cgtaaatttg cagaaaaaac tgactcaaaa aaattgccag ttcaagtaaa
     3061 gtgagatgag agcatgttaa atttttttag aaaaaatgaa ataagcgttg tagcacctgc
     3121 aaccggtgtt tttgaaaaac tggagaaatt aagtgatcca gtcttttcaa aaggaatgat
     3181 gggacaagga tttgcaattg atgcaaaaaa cgaaacgatt gtatctccaa tcgatggttt
     3241 tgtttcaagt atttttccta caaaacatgc cttaggaata aaaacaaaat caggaattga
     3301 gatacttgtt catgtaggaa tagatacagt tgagctaaat ggtgagggat ttgatattaa
     3361 agttcaagaa ggacaaaaag ttaaggctgg ggatattctt atgaccatta attttgtagt
     3421 ttttgaaaag aatcacaaga ctaaagatgt cattgttgtt tttccagact accaagacga
     3481 aattttcatt ccgaaacttg aagaagtaaa acaaggacaa gaattgtttg ttcttggcta
     3541 atgataagta attaataata gaatcgagga aaatgtatgg tttttggggc tattgaagct
     3601 ggtggaacaa aatttgtttg tgccattgga gataaagatt tgaacatatt tgaaaaagtc
     3661 agctttccca cgactacacc caaagaaaca atgaatttag ttttagattt ttttaatcat
     3721 cataaagatg atttagaagg tatcggagtg ggatcgttcg gtcctattga tattcattgt
     3781 gaatctaaaa cgtatggctt cattacatca acgccaaaac ttgcttggca ggattttgat
     3841 tttattggaa gtttaaaaaa gcaacttaat attccaactg cttggacgac tgatgtaaat
     3901 gcctcttgct atggtgaata tgtttctgga aatggcagag gcctttcaag tgttgtttac
     3961 tataccgtag gaacgggcat aggaggtggc gctatccaaa acggaaactt cattgaaggt
     4021 tttagtcatc cagaaatggg acatatgctt gtaaaacgac acaataaaga caacttttct
     4081 gggacatgtc cattccacgg agactgttta gagggcttag ctgctggtcc ggcaatcgaa
     4141 agaagatcag ggaaaaaggg acaagaagtg actgaaaatg acgttacttg ggaaatagaa
     4201 gcttattaca ttgctcagtg tgcatataat acaacattga tgttatcacc tgatgtcatt
     4261 atatttggtg gcggagtaat gaaacaaaaa cacttaaaaa agaaagtaca agataagttt
     4321 attgagttga tcaacggata tgtcgctatt cccgatgttg acaattatat tatgacacca
     4381 aaattgttag ataattctgg tattgtaggt tgtttagctt tagcaagaga cgttaaagaa
     4441 aaatcttaat ttggaaaaaa gatcgtctcg actatttgtt tggttgatgc gatctttttt
     4501 ttcaattagt aattgaaggg acgcctaaaa gtgaaaaaaa aatgaaagat gaagtattag
     4561 agtatatcaa aaataattta aagtattacg atttttctgc tcaagatatt gcaatgaaat
     4621 tttgtattaa aagaaatgtc gcttcccact atttgaatca gttattttca gatggaaaat
     4681 tactaaagaa tgattctgtt agaccagtaa tgttcaagta taatcaacaa aagccaaaag
     4741 actgcttttc taagtttatt ggagcagaca tatctttgaa atccactata gataaatgca
     4801 aagctactgt aatgtatccc cctaatggcc tcccattaat aataaaagga aacagtggcg
     4861 ttggaaaaag ttttttagca agtttgatct atcagtatgc tcttgataga aaagtaattc
     4921 ataatgacgc aaaatttgtt gttgtgaatt gtgcagatta tgcaaataat ccagagctat
     4981 tgtcagcagt tttatttgga tataaaaaag gagcctttac tggggctgaa aaagatactg
     5041 cagggatgct atcttctgct aatggaggat accttttttt agatgaggtt cacaatctaa
     5101 gtgcagaaaa ccaagagaaa ttgtttcttc ttatggattc tcaaaagtat cgtaaacttg
     5161 gtgaaagtgt gcaatgggag tatgcaaatg ttcgccttat cttagccaca acagaagata
     5221 aaaatacctc tttgttagca acttttagaa gacgcatccc agctgaaatc actctgccag
     5281 attacaataa tagaagtaca tctgaaaaaa ttcaattatt atttgaattt cttaaagatg
     5341 aagcaattac aattgattca aaaatatttt gttcgattga actttttact gatcttttat
     5401 cgaaaacatt tgaaggaaat gttggagaat tgaggaatga aatcaagctt ctttgtgcag
     5461 aaggttattt aggaaataag agaaagtctt caatttattt aggggacaaa ctagacagcg
     5521 gtttttggat agatccagaa attagtatag atttaaaaaa aatgtttctt aaatcacttt
     5581 ctgaaattga tttgattgaa ctttttcaaa atcacatttc aattgaagaa agcatgagtc
     5641 aattaagaga tagaatactt aaagagtgtg caggttcttc tgagtataat tatttagaaa
     5701 gtgacgaatt tatagcatta agaaactatg ttgttaataa aatatcacca attattgatt
     5761 cgacaggtct atgtttatta gatgaattct tggcggatat cagtctattt ataatgttta
     5821 ttgatgtaat tgaagaaaca tcaagaaatt ttttgctgtc taagttcaga aaatttcgta
     5881 tgaaaaatga taaacaaaaa ttattggcaa atgagatttg gagtagttta gaagtcgaag
     5941 aaagtaaaca agaacttttg ttgaaatgga tactttttct tgtaaagaag ttctatgtga
     6001 aaataccaga aacccattgt ctgatagtta tgcacggtaa aataacagct tctgcgatcg
     6061 caagggaaac aaataagttg ctgaatacat atgtttatga ggctttcgat atgccaattg
     6121 aaggtgagac tagtgactta attaacctaa tcaacaattt ttgtaaaagt atagatacga
     6181 cgaatggact cattctgcta gttgatatgg gatcattgga acaaatgtat gaaaaaattg
     6241 aatccaatgt aataggtgat ttggtaattt taaacaatgt ttctacagct ttagcgattg
     6301 aatgtgggat tcaagtttgc caaaaaaagc caattagtca tttttaccag atggattttg
     6361 attcgtttca agttaaagtt cagtattata aaggattatc acaaaagaaa aatgtaatag
     6421 tatcttgtct ctctggtgag gggatttctg aaaaagttaa ggatatttta aaacggtatc
     6481 tcgataatca agtggagatt ttgatttttg attttaacgc attaaagaaa atagcaaagg
     6541 aaaaagatac tatgattttt aagaatacaa tttgtgttct ttcaaccact gaattttaca
     6601 ttcaaggaat cgattgttta aatttagaaa atctaattaa tgggaatcaa accttagaaa
     6661 aattaaataa gtatatggac tcggatagct gtgagttctg cttaaatgaa ttagttaaac
     6721 tctttactat tgaaggcgct agtactaagc ttcgcttttt agattccaaa aaagtattca
     6781 atgagataga aaaagtacta tatttgtatg aaaaatatta cgatgtcaaa atcccaagtt
     6841 ttctgagaat taatctattt ctacatcttt ctggaatgat tgaacgaatt atgattggtg
     6901 atggaataac aaatgaacat ttgaaagagg gagcgcaact atttgatact tttttaaatg
     6961 tatcaaaaga ttttttttca gaaatcaaag atatttactg tattgaaata cccaaaggag
     7021 agtatgagtt aatctataca atttttgagc agactatttt ctaaatagca tactagcgtc
     7081 taaaaaaatg aaagggtgtc caaagtatat ttagacgctc atttaattaa tatttactgt
     7141 attttcccgc tttattggat aatgttagtt ggcacagtaa ttgcttatat aaaagtataa
     7201 gaagaaagga gagaacaaaa tggcgttttg gcaaacattt ttagttgtgt tgtatggatt
     7261 tttcattaac tgggataagg aagggcccaa aatcggaacc tcccaacctg tcgttgcagg
     7321 tttcttgacc ggacttatta tgggagatat tcaaactgga ctttatatcg gtgggacttt
     7381 gcaattaatg acactaggta ttactagttt tggtggtgca tcagtaccgg attatcaaac
     7441 agcagcattg atcggtactt atttagcaat ctcaactaac caggatgctt ctataggaat
     7501 aactttagca ataccggtag caatgttaat tgtacaacta gatgttctaa agtggtcaac
     7561 aaacattttt tttcaacaaa aggcagaaaa attcgcacaa aaagaagatt atcaaaaatt
     7621 agagtggatg caatatttgg catcaattaa tacaagcttt gtatctggaa ttcctgtttt
     7681 gttaactgtg atattaggcc caacgttagt gggaagtgca atcaattata ttcctgaatg
     7741 ggttaccggt ggtttaaagg tagcaggggg actattacct gctgttggga taggaatgct
     7801 acttaaatat ttacctacaa aagattactt tgcctatttg ttagttggtt tcgttttggc
     7861 agtttatttc caaataccaa ttttagggat ttcgttactt ggcgctgcaa tcgcactaat
     7921 cgtatataaa aatagatcaa ataatactca agtagcacca gctgcaacag gaggttttga
     7981 cgaagatgaa taagaaactg acaaaaatcg acaaacaagt aattttccgg tggattctca
     8041 atggtgcaac ttcattaaat tatgaaaaaa tggaagggtt gggttatgcg tattctatgc
     8101 ttccttttat caaggacaca tataaagatg atcctgaagg aatgaaacaa tccgttttag
     8161 ctcacctaca attttttaac actacgccgt atacagcgcc ttacattatt ggtatgaatg
     8221 ttggaattga aaaagaagaa aaaaataagt ctttggaagc agtaagtgct ctgaaaacag
     8281 gtctaatggg tcctttagcg ggaattggag atagtgtatt tgtagttatt ccatggacta
     8341 tttttggagc aattgcagct aatatggcat tggaaggtag ttttgttgga attattttgt
     8401 ggatactggc atcaatttta ttaaggatta ctgcttatcc tttatacctt gctgggaaag
     8461 aatcgggtgc gagcctacta actcaaattg aaacaaaatt aaagtctatt actgatgcag
     8521 tatcaatttt agggttaatg gtggtcggtg cactcattgc cacagttgta aaagtgaatg
     8581 ttggctttga atttaaacaa ggcgacttaa caatgaccgg aaatgaaatc tttgatcaag
     8641 ttatgcctgg tttattacct gcatgtgtag tagcaattgt gtattatttg ttaggacgaa
     8701 aggttaaacc agtttatgta attctattaa tcttagtagt ttctattgcg ttaagagcgt
     8761 taaatatttt gaagtaggtg ttgaatatgg atgaaaaaat gaaaggtatt atacatgttc
     8821 gtattgatga tcgaatgatt catggacaag tagcaacaca atggacaaat caattgagtg
     8881 ctaacagaat tatggttatt aataatgaag tagccaatga tgacgtaaaa aaagctgtag
     8941 ttcgcctcgc tgcaccacct aatgttagaa cttcaataat tacaagggaa gtagcagtta
     9001 aaaatattct ttctggtaaa tatgaaggac aaaaagttct tattgttgca gtttctccaa
     9061 tcgatgttaa atttttgatt gataacggtt taccaataac aagtgtaaac gtaggaaatt
     9121 tatcaagaag gaatggaaca gaacgcatcc gtccgacgat aaatattact gaagaagaga
     9181 gagaggcttt taaagaacta attagtgatg gagttgaagt gacagtgata caaactccta
     9241 gagattcaaa agaattttta aaggatttta tttattaaga acagaggatg agtggatgga
     9301 gtttataatt acatctcatg gaaatattgc cattggattt gtagatacat taaaaatgtt
     9361 ttttggcgat aatttaaatt gtcatgtggt aacacttgga gatgcaggca ttgaagaatt
     9421 ccgtgaaaac attgaaaaaa tagtttctca agtaaaaaat caagaagtta tggtatttgc
     9481 tgacttgatt ggtggaacac ctttcaacga atttgctaaa agaagtgaag tatttcaaaa
     9541 tgggtttcaa cttcttggtg gcatgaattt aggaattttg attgaggctg tcaatttgag
     9601 attacaagga aaaaaaagcg atgatatcat cgaaaatcta agaaatatga atacaattgc
     9661 gtgtcttgca gatatgcaaa gacatactaa cgaagaggat gagtgataaa tgcaaaaaat
     9721 tttttacaag cctaaagatt tttggtttgg ggattgcatg cctttttata aagatggaac
     9781 tttttatcta tttcatcaac gagatacgag aaatcctaaa cctttaactg atccttttgg
     9841 gtggtcacta gttacaacca ctgattttgt gaaatttgaa gaatacgggg aagtaattca
     9901 tcgtggtggc aacgatgaca ttgatcaata catttacgct ggaagtattg tccaaggaat
     9961 caatgagtat ttagctttgt atactggaca taatcgcaaa gctaagttgg atggaaaaac
    10021 ttcagaagtg ttaatgattg caagttctaa agatttaatt cattggaaaa aggatggcga
    10081 agcagcaaaa cttgttcctc aagaaggata tgataagaat gattggcgcg atccttttgc
    10141 aatttttgat caagatcatg gaaaatacgt tttgatacta ggagctagaa aaccagcaag
    10201 taagaaaaag ccgacgggga gattggtcta ttttgaatcc gaagatttaa aagattggaa
    10261 tttcaaagga gactactggg ctaataatga atttaacatg attgaaatgc ctgatatatt
    10321 tgaaattgat ggaacgtggt atttactttt ttcagagtat tgtgaagata aaaagactaa
    10381 atatcgagtc ggagcaggat tattttcaga ttggaaatca ccagtagacg aagctttcga
    10441 cggaaaagcc tattatgcag caagaacagt cggaactaat gaaactggcc gttttctaac
    10501 tggctgggtg gcaacaaaag aaggggagaa cgatcaaaat aactgggatt ggggcggtgc
    10561 acttgttcca catcaaatta ttcaaaagac agacaaaact cttggtgtta aactgcctga
    10621 aaaaataatt gagaatttta aagaaatcac tccattttca agttttaagt tagagaaaga
    10681 atttggaaaa aaagaaagag taattgttcc taaaatcgaa gattattttc tatttgaaac
    10741 aactttgact attacatcgt taacaaagga gtttgcttta aaattatttg tagatagtga
    10801 tactcaagaa ggatacgaat ttcgtatttc attgattaat aatcagctta ctttcggaaa
    10861 aactccgaac tatccttggc ctcaaatgtt tgataagggg ctgcaacgac cactattttt
    10921 ggaaataggc aaagaatatg aactaaagtt aattgttgat gatacgatag ctgttttata
    10981 tattgataat gttgctttaa gtactagaat gtatgataaa gtcggaacag cattaagtat
    11041 gactgttacg gagggagcaa tcaaggtaga taaactagac tattcaataa attatgaatg
    11101 tggattttaa aacaattgaa gggatgatgt aagtgaaatt aaatggttta caacatattg
    11161 gaattccaac aaaagatttt ggagcttcaa aaatctttta tgaatcctta gggtttagtc
    11221 tgattaatga agaaatgaat gtgggaagta aagtcggctt tttcgagttg aatggaacga
    11281 taatagaaat atgggaagat aaaactactg agtgtagagg agccattgat catctagcat
    11341 tagatacgga tgacatagat gaggtattca attcaattca agatttagga tatgaattat
    11401 tagataaaaa tattatggaa ttaccgttct gggaaaatgg aatccgttac tttaatttct
    11461 acggaccaaa tcatgagatt atagagataa gccaaatgaa gaaataaata gataacgaag
    11521 tgctctaagt tggtatgaac gaaaagtact tcattttttg gtatcagcat ttcaatagtt
    11581 taatgagaat ttactatttc aaattaattc aaagtgacag tattttttac ttatttggtt
    11641 tcaaattttg taatccttcc tatttattgg aatacactcc acattttgta tacaagtcat
    11701 atcatgtttt agcaaagctc ttaccaagca aagttataat gatgttttcc aaagtttgat
    11761 ctttcgatgg aggctaacac atattttttg caggacagcc aaaattagat gtatagaata
    11821 gagtctgtga ggcgtataga cagtttattg tctgtatgcc tttttttatt ttgaaaaatt
    11881 tttagtattt ttcgaaaaat gctcactcct agagtaagcg ccccatagaa cagtacctta
    11941 ctaacaaatg cacctcaaga tatacttgag ctatcaagaa gtctggaggt tttct
//
LOCUS       DACEKH010000084        11700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEKH010000084 DACEKH010000000
VERSION     DACEKH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.087"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..586)
                     /locus_tag="IW662_002131"
     CDS             complement(5..586)
                     /locus_tag="IW662_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0866946.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IW662_002132"
     CDS             complement(769..1395)
                     /locus_tag="IW662_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0866947.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IW662_002133"
     CDS             complement(1417..2748)
                     /locus_tag="IW662_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ0866948.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IW662_002134"
     CDS             complement(2903..3304)
                     /locus_tag="IW662_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0866949.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IW662_002135"
     CDS             complement(3390..3596)
                     /locus_tag="IW662_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866950.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IW662_002136"
     CDS             complement(3691..5193)
                     /locus_tag="IW662_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ0866951.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW662_002137"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW662_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0866952.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="IW662_002138"
     CDS             complement(5764..6381)
                     /locus_tag="IW662_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866953.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="IW662_002139"
     CDS             6602..7975
                     /locus_tag="IW662_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0866954.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="IW662_002140"
     CDS             8050..9162
                     /locus_tag="IW662_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ0866955.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="IW662_002141"
     CDS             complement(9234..10871)
                     /locus_tag="IW662_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ0866956.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="IW662_002142"
     CDS             complement(11039..11647)
                     /locus_tag="IW662_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0866957.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3074 a         2468 c         2120 g         4038 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggatag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacaaccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
//
LOCUS       DACEKH010000085        11515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEKH010000085 DACEKH010000000
VERSION     DACEKH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.088"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(251..493)
                     /gene="acpP"
                     /locus_tag="IW662_002143"
     CDS             complement(251..493)
                     /gene="acpP"
                     /locus_tag="IW662_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0866958.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(615..1616)
                     /gene="plsX"
                     /locus_tag="IW662_002144"
     CDS             complement(615..1616)
                     /gene="plsX"
                     /locus_tag="IW662_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ0866959.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1665..3701)
                     /gene="recG"
                     /locus_tag="IW662_002145"
     CDS             complement(1665..3701)
                     /gene="recG"
                     /locus_tag="IW662_002145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ0866960.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3818..5500)
                     /locus_tag="IW662_002146"
     CDS             complement(3818..5500)
                     /locus_tag="IW662_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ0866961.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5552..5914)
                     /locus_tag="IW662_002147"
     CDS             complement(5552..5914)
                     /locus_tag="IW662_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0866962.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            6184..6372
                     /locus_tag="IW662_002148"
     CDS             6184..6372
                     /locus_tag="IW662_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ0866963.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6610..9279
                     /locus_tag="IW662_002149"
     CDS             6610..9279
                     /locus_tag="IW662_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0866964.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(9370..9990)
                     /locus_tag="IW662_002150"
     CDS             complement(9370..9990)
                     /locus_tag="IW662_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0866965.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            10102..10503
                     /locus_tag="IW662_002151"
     CDS             10102..10503
                     /locus_tag="IW662_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866966.1"
                     /translation="MKKLFSALFILLFGFIGIGTSENIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            10638..10976
                     /locus_tag="IW662_002152"
     CDS             10638..10976
                     /locus_tag="IW662_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAQ0866967.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
     gene            complement(11357..>11515)
                     /locus_tag="IW662_002153"
     CDS             complement(11357..>11515)
                     /locus_tag="IW662_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAQ0866968.1"
                     /translation="IKNHEKFLIRNYKISGGGCYLLECRFTSKKSMNSFLEQLNEYAN
                     YKLSIVID"
BASE COUNT         3311 a         2305 c         2031 g         3868 t
ORIGIN      
        1 cttcctaaat ggaagttttt cttaataaaa aaggacatta cacagaataa agcgttttat
       61 aacaaaggtt attcttatct ttgtgtaatg aattattaat aattatatag aaaatcgctt
      121 tactttgcaa cataatttct gtttatcgcc agaattatca gaaaaatgac ttttacgtgt
      181 caaaaagatt aaaaaaagaa ggataaaaag ctgtatgcag ctttttatcc ttctttgctt
      241 ttatttgaaa ttatttaaga tggctgctga tataatctac tgcgccaccg actgtttcaa
      301 tttgttcagc gtcttcatct gaaatttccg taccgaattc atcttcaagc tctaaaacga
      361 attccataat actaattgaa tcagctttta aatcatcttt gatgttcaat tcatccgtca
      421 ctttatcggt atctatctca aaatgatttg aaataatttt cgctactttg ttaaatactt
      481 cttcgcgagt caactttttc acctccaaga atcatacact tgtcaattac ctacaccatg
      541 gctaatttta ctggcatttt tcaggaatgt ccaccttttt ttcaatggaa cggattgctt
      601 cagatatcca aatactattc ttcgcttttt tcaaattgtt cgactaattg tccaactaca
      661 tctgtctcaa gcattgtgtg gatttggcgg atcgtatagc gaacagcatc ggggcctgtt
      721 gctccatgag ttttaatgac tggtgctttc aagccaaaca aaacggcacc gccatgtttg
      781 gaataatcca tttctgcttt caaagaacgt aagccgtctt ttaaaagcat agcgcccatt
      841 tttcctttga tcccttcatt tagaatagca gattttaata gattcatcat attcattgct
      901 gttccttcaa tagatttcaa aacagcgttg cctgtaaatc catctgtgac cactacatca
      961 gccacaccat tgagtagctc acgagcttct acgtttccaa taaaattcaa gcttgtttca
     1021 ttctgaagga gttcataggc ttttttcgtc acttcactac cttttgttgc ttctgttccg
     1081 ttgtttaaga gtgcgacacg aggacgtccg atacctcgga ctttacgtgc ataaaaagaa
     1141 cctaaaatac cgtattgcaa caaatgttct ggtttgtttt ctgcgttagc gccaagatcc
     1201 agcatatcga aaccggcacc ttctttccct actactggca aggtagacat caagccaggg
     1261 cgttcgatat ttttgatacg tcctacgatg aacaaccctg ctgctagtag cgcgcctgta
     1321 ttccctgctg aaaatacagc atctgcttca ccgtttttca ctgcttgtgc agcaaggacc
     1381 attgatgcag tcttttttcg tcgaattgct tttactggct catcatcact attgattttt
     1441 tcgtctgtat ggataatcgt gatgtttttt tcatttgtta agtatttctt gatttctgct
     1501 tcttttccgt aaagtaagaa ttctacttct ggaaaatctt gctgcgctaa agccacgcct
     1561 tctacaattg cttgaggtgc gtgatctccg cccattgcat ctacggcaat tttcattgtt
     1621 tccactcctt tttccgtcat cagtataaag tatagcacag tctgctaatc gaaataacga
     1681 ttatcctgat tttctatttg gaggctttgc gctaaaggct gatactcagg aagcatctgc
     1741 caatccttta tctgccaaag ggctgcagcc tcttctctgg ctacctccaa aacatttcca
     1801 tccgtcacca agtcagcaat cgcaaattga ggcagaccag attgacggaa gccaaatact
     1861 tcaccaggac cacgtaattc cagatctttt tcgcttagta caaaaccatt ggtggtctct
     1921 gtcatgattt tcatccgttc cacacctagt tcatttttag gattagcaac taagatacag
     1981 taggaagcat cacttccgcg tccaacacgt ccacgaagtt gatgcagctg tgccaatcca
     2041 aatcgatccg catccatgat cagcatgacg gtcgcgttcg gcacgtttac ccctacttca
     2101 attacagtag tcgaaactaa tatctgcatc tggttgtctt tgaaggcttc catgattact
     2161 tctttttcct gattcttcat tttaccatgt aacaagccaa cttcatattt tggtgcaaat
     2221 agttcacgaa gtttttcata gatttccact gcatttttta catctaatgc ttctgattct
     2281 tcaatcagcg gacagatgac atacatttga tgtcctcttg ccagttcttt tttcgtccaa
     2341 tccagtacac tgtccaattg aggtgttctt acccagcgtg tttcgatcgg gatacgtcca
     2401 gctggaagtt catcaatgat agatacatcc atctcaccat atgctgtaat agctaatgtt
     2461 ctaggaatcg gagtcgctgt cataaataag acatctggtc gccagccttt ttcacgaagt
     2521 actttccgct ggttcacgcc aaaacggtgc tgctcatccg taataactag acctaaattc
     2581 tgaaattcga ctccatcttg gatcaatgca tgtgtgccga tcacgatgtt aatttcccct
     2641 tgctctagtt tttcaaggag ctcacgacgt tcttttgctt tcgttgaccc cgtaagcaat
     2701 gcgatggtta cttcattggg atcaaacagc tgttgcaagc tttccatatg ttgttgtgcc
     2761 aagatttctg taggcaccat caacgcacct tggaaacctg ctgtaacggt agcgtataac
     2821 gcaatcgcgg caaccactgt ttttccgcta cctacatctc cctgcaaaag tcgctgcata
     2881 tgctgcggac tgcgaagatc ccgacagatt tcattcgtta cacgtttttg tgcatttgtc
     2941 agttcaaaag gtagcttttg agtaaattct ttcaattttt ggacatcata aaggatctcc
     3001 aaaccattcg tttctgcttt ttctgctttt ttcaatcctt gaagacgcat ttgaaataaa
     3061 aagaactctt caaaaacgac acgtcgtttg gcttgatggt tttcttcgtg gtcttttgga
     3121 aaatgcatac ttcttacagc agaagctcgg tcaagcagtc gatatttctc cacaaggttg
     3181 tttggcagat tttcttcaat caatgtgccg tattcttgaa accctttttt gattagatca
     3241 actaatgtcg tttgacgaat tgatttgttg acgtgatata tcggcgaaaa gtcatccatt
     3301 gtttgagaac ctaaaatctt catcccgttc aatgctttac gctttgcatc ccatttaccg
     3361 tatacggcga tttcctccga taaaatgact ttttctttta gataaggttg attaaaaaaa
     3421 gaaacgttga acacatcatg ttcttgcatc atacgaaatt gcaatcggct ttttttataa
     3481 ccaaatcgat tcatcacagg aggcgagact acgattcctt tcaaagtaac cttttcttga
     3541 tcttggatct cgtttagatt tctttcctga atatcttcgt agcggaaagg ataataagtc
     3601 agtaagtctt caatcgtttg gatgcccaag ctggcaagac tagctgctct tttttctccg
     3661 actcctgcca gcatagtgat tggatcatct aaagaacgca taaatttttc ctccctccct
     3721 taacaaaaaa aggacttggt cagcgatcat acctgatcac taaccgaagg ccttttatga
     3781 atgaatactg tgttagaaag atattctgac acagaaatta ttcagcagaa aatagataag
     3841 gatagacagg ctgatctcct tggtggatct cgatttccaa atcaggatgt ttttctgtta
     3901 gtttctcagc aaattcattt gcttgttcaa ctgttccttc atcacctgcc aagatcgtta
     3961 cgatctctgt gtcctcattg atcatttgtt ctagtgtact taacgctgtt gctgtgatat
     4021 ctgggttaga gatcaggatt tttccatcga tcatgcccaa aaagtctcct tctttgatct
     4081 ctacaccgtc aattgaggta tctctgatcg cgtgagtgac ttgaccgctc acaacgctag
     4141 acaacatttc tgtcatcgtt tctttgtttt cttctaaaga agcttgatca ttgaatgaaa
     4201 gcatagctgt caatccttgg gaaatcgttc ttgaaggaac aactgctact gggatatctg
     4261 ctacttctgc tgcttgatca gctgccatga agatgttctt attgtttggc aagacaatca
     4321 ctttatctgc gtttacttct tgaatcgctt taacaatgtc ttccgtactt ggattcattg
     4381 tttgcccacc actgataaca taattagcgc ctaaactttc aaacagctct tggacgcctt
     4441 taccagcagc gatcgctacg ataccatacg ggatacgtgg tgtttctttt ttcgcaaact
     4501 cgttcacttc ttcatcgtgt tccaaaatcg tctcatgctg caagcgcatg ttgtcgactt
     4561 tgattttgac taatgaaccg aatttttgtc cataattcaa gacttctcct gggttttctg
     4621 tatgcacgtg gactttgatg atctcgtcat cattgaccac aagtaaagaa tctcctatac
     4681 catctaaata gttacggaat tcttcgtaat cgaatgtact atcgactgta ggtccttctc
     4741 cgatttttac catgatttct gtacagtaac caaaatggat atcttctgtc gccatttgac
     4801 cttggacact gcgatgatgt tctgcattga ccatatcatc catttctgct ggagaaggtt
     4861 catatgtttc atccgcttgg aattctccat tcaatgcact taaaaagccc tcataaacaa
     4921 acagcagtcc ttgccctcca ctgtctacga caccgacttc ttttaggact ggaagcaagt
     4981 ctggagtttt agctaaagca cgctttgccc cttttacaac agctgtcatt acttcaacga
     5041 catcatcagt actttttgct ttcttttctc cagctttcgc tgcttctcta gctactgtca
     5101 aaatcgttcc ttctactggt ttcattactg ctttatacgc agtttcaact ccatgagtga
     5161 aggctgcagc aagatcggaa gcatttaatg agacaacatc tggaatttgc ttagaaaatc
     5221 cacgaaacaa ttgagacaaa ataacgccgg aatttccgcg cgcacccatc agcaatcctt
     5281 ttgaaagcat tgctgctaat tctcctactt tttctgaggt tgaatctact actgccttag
     5341 ctccactagt catagataga ttcatatttg ttcccgtatc gccatctggc acagggaata
     5401 catttaatga attgacgtat tctgcattta cttgcagacg attcgcacct gcctggacca
     5461 tttcctggaa ctggcttgca cttatttctg ttacactcac ctagataatc ctccttcaga
     5521 tcgactgctg agttaccagc cagtctcctg cctagtctgg cagtacacga acaccttgta
     5581 caaagacatt aactgaattg gcagttacgc ctaataatgc ctctagattg tatttcactt
     5641 tttcttggac gttgcgggaa acttcagaga ttttcgtacc atagctgata atcgtataaa
     5701 catctacagc aataccatta tcttcttggc ggacaactac tcctcttgag taattttctt
     5761 tacgcaaaat ctcagtaata ttatctttaa tttgattttt gctggccatt cctacaacac
     5821 cgaacacatc tgtcgcggcg ccaccaacaa ctgttgcaat tacatcattg gtaatttcaa
     5881 tggttccagc tgacgtttga atttttacag ccatgaatga gcctccttaa cgggtaaaaa
     5941 actcccattt ttcttctatc atattttatc atagcgcaac ttgaaataaa aggcagttac
     6001 ccaaagtact caaaaacctt gcaaaatttc aggtataacc ttgtttaatt tacaattatt
     6061 cttctcaatt gtcaattgta tctatgaaaa tgtacttgca aatttccatt ctatatgata
     6121 tgttagtctg gtatgagctg aattattcta ttgctccaaa tcaaggcaaa ggaggaaatt
     6181 accatggcaa aagtttgtta ctttacaggt cgcaagacaa aaagtggaaa caaccgttca
     6241 cacgctatga actcaacaaa acgtactgtt aaacctaact tacaaaaagt tcgcgttatg
     6301 gtcgacggaa aacctaaaaa agtttgggtg tcaactcgtg ctttgaaatc aggaaaagtt
     6361 gaacgcgttt aattgaatga tacttggacc gttcagatag tgaacggttt tttatttaat
     6421 aaaaattttt aagggtggga cttttgattc ctaccctttt tttgcgttta tttttgttat
     6481 acgaacaaat tatgaagtta tttattctaa attttatttt tttgtgaaaa ttagactctt
     6541 tttaagcaga gaaaaacaag gatatgataa actgaataca acttcataga aatttggtgg
     6601 aaaataaata tgaaaaaaaa tttaaagtca ttctttagag aaaacggacg ctacgcagca
     6661 gctagtttcc tgattccgtt tttgatttta gcacttgttt atctaagtat cgggatctat
     6721 cctggaagca gcagaagtat tttggcaagt gatgcctttt cacagttttc taattttcat
     6781 gccagcttcc gaaatgttct tcttggaaag caaagtattt tttatacatg gaatgcttct
     6841 ctaggattga attatttatc tttaatttct tactatctag gcgggatctt cacaccactg
     6901 gtgttctttt ttccaaacca gatgatgcca gatgcactgt atgttctgac tttgcttaag
     6961 gtcggctgtg ccggattggc tttttggttt cttgcacgta cgttcaagat tcctcgctgg
     7021 gggcatgttg cacttagcgt cagttacgca ttgatctcat ttatcactgc acattcggag
     7081 atcatcatgt ggctggatgc atttgtttat cttccattag tgattttggg gattcatcgt
     7141 gttatggatc ttaaacggcc gaaactttta tttatcagtt atttactctt gtttttatcc
     7201 agtttttata tgggttttat gattggcgta ttttctttct tatattttat cgcccgtcta
     7261 ttgacaaact ggtcagaata taaaaaaacg attgttcctt atggtatcac ttctttactg
     7321 gcaggcggcg cttcaatgat cattgtattg ccagcagtgc tggatcttcg gtcaaatggc
     7381 gaggcactga gtcagatcac tacgttgaaa acagaagcga cttcttatct ggatctaatc
     7441 atgaaaaata tgattggtgt ttatgacacg acaaaatacg gatcgattcc gttcgtttac
     7501 gcaggtctgc taccattagt cctctgtctg ttttattttg ccagtcggaa agtaccgttg
     7561 aaaaataaat ttttgtacgg cagtcttttc gccatactta tcgcaagttt ctatcttgtt
     7621 ccattgaatt tgttctggca tggtatgcat gcaccgaata tgtttttatt ccgttatgcc
     7681 tatctgttct ctttcttagt gctcctgctt gcaggaaaag gatgggaaaa gatagaacaa
     7741 gacgatcgtg ggctgttgtt aggaatcatt ttggtcgtag ctgctctctt tgcggtggca
     7801 tggggcatca aacctgaagg aagctacacc tatgtgacga tgacttcctt tgtattgaca
     7861 gtggttttct tagggattta tgcttttgct gtgggatttt atcagcataa aaaactttca
     7921 ttcaatcatc tgagtatatt gttattattg atcatgtctg ctgaagcact ggtcaatacg
     7981 aacagtatga tccatggcat tcttgatgat tggaattatg cttcccgcag tttatatact
     8041 gatccttatc ctgacttgaa agctcttgta aatgaaacaa aaaaagattc cgactcgttt
     8101 taccgcttgg aaaatctgga tccagtatct gcaaacgatt cgatcaacta tggttacagt
     8161 ggtatcagtt tgttttcttc tataagaaat cgtaattctt cctcttacct ggataaatta
     8221 ggtttccgtt caagagggac aaatttgaat atccgttata acaataatac tttgctaatg
     8281 gatgggttta caggaatcaa atataacatt tctaaaaacg aaagcacgtt cagtaaatat
     8341 ggcttccgcc aagctggtca aagtggcgac tatacactgt atcagaatgt caatgcattg
     8401 ccactagcat ttactgcacc gttgtccgtt aatgatgttg agcagccagt gacagataat
     8461 ttgaccagtc agacaaactt gatcaataca ttgtctggtt taaatgaaca gtattttaca
     8521 ttctatacac ctactttgaa gaatcagttg aatacgacga tcacaaagac aacgactggc
     8581 gtcacttact cagaaacagc agataaccag ccaaaagagc tcacttggga agtccatgtg
     8641 cctgcaaata cacaagctta cttaagtttg ttcccaacca attttgcaca attagaaagt
     8701 tctagtgcga cgatcactgt caatggtaca tcgcgaaaaa cgcaaatcaa catttctggt
     8761 caatactatg atcttggcta ttatcctcaa gagacaacag tgacatttac tgcaagtttt
     8821 tatggaacga aagaagtaag tttcatggag cctaaagtcg ttggtctaga tgttgtagcc
     8881 tatcaagccg caatgaatca gatcaaagaa aaaggtgtcg aaatggaagc cactggcaga
     8941 aaagcttctg ggactgtaaa taccgctgaa gattccatgt tggtgacaac tattccttat
     9001 gacaaaggat ggaaagctaa aattgatggg aaaccagtca aggtcagcaa tttcaaagac
     9061 gcattcttga tgataaaagt tccagcaggt aaacatactg tcactttcag ttacttgcct
     9121 gaaggattta cgataggtgc aatccttttt gtcctatgta ttgcattgtt tattctttat
     9181 gtctggtacc ttgaccgcaa tcgccccttt tatggcacga ttgatccagc aatagaagaa
     9241 aaaccgcgaa atacacacag aagaagacga agaaaataaa gaaacgatgg aatctgtgtc
     9301 actcttctat ttaaatggca aaaatttcaa tctttaaatg acaaaaacca cctgattttt
     9361 ggtggttttt tatataccta tgactaaact atctcttgct gcttcgacaa cttctacagt
     9421 gatcttatcc atttgattga ttcttaaaat ccgatcaatc tgcgcaaata aacgatgaat
     9481 aagcctaaaa tttccttttg tgatcttaat gatagttgtg atagcctcat aatcagaaaa
     9541 atcttctagc ttgatatcaa ttcctaaatc tgcccatttg tattccaaaa tatgatgtgt
     9601 ttcgtcctta ctaaggttat caaattcatg tgcaaatcct atgcgagagt aaagctgcgg
     9661 ataacgagac aatctttttt caataccagg cataccgata agaaccattg ccagatcatt
     9721 tctatcgtaa atatctctta gctgttctaa atgctgaagc tttaaacgat caatttcatc
     9781 gacaataatt aagtcaattg cttcataggt tttaggcaca ggaggatctt cttgattgta
     9841 ttttagtacc atatgcattg ctttagtcag acctattcta ctagttattg tgttaatatc
     9901 cgaactgaca cgagacatct tttcagctgg tacagtataa aaaactttat ttacacttaa
     9961 tatttcatct gttgcattta tcaagaacaa gaaaaagtct gaatattcaa aaaataacgg
    10021 tgtacatttt taaaaggaga tgtataatcg agatttagaa agcgttttcc taaaaattta
    10081 atgaattagg aagtgattac tatgaaaaaa ttatttagcg cactttttat tctgttattt
    10141 ggctttattg gtattgggac ttctgaaaat atagttaaag ctagcacgat ggttgaacaa
    10201 acaagtgaaa tggcaaaaac ttttagtcaa ttaacaccag aagaacaaag ttacttcaaa
    10261 tccaaaggat ttagtgataa agatactttt tacactcaaa agatagagcc tcaagatccg
    10321 aaaacacgtt tttctgcaaa tgtattgcgg ttaaccgcat ctactaaaaa aattactaat
    10381 acgcaggggc ggacagaata tattataaca agtcccaatt cggcgatgta tcgtatttct
    10441 acacgcttga gtttaggtag tgcaaaatca tatactcaaa cagtaaatct agctaattct
    10501 taaggttatg gtggaggaat gtattttaca tttactggga aaagacaata ttacagtgta
    10561 aaattagcta tgcaagttta taatacattt ggtgtagggt cagtctcttg cactgcggga
    10621 ggattgacaa taggaaaatg ataatttctt actatgtctt aaatagaagt gatgatgaag
    10681 gactcttcgt ttttcaatca ttacatgagg atacgatgct aaaagagttt tataatcgga
    10741 ttaagaataa acagatagaa acaaatttat attttttaag tgactctatc ttgcctttta
    10801 ttagcaaccc taaaattctt aacttgttgc agacaaaaga tttgaaaagt ttaccgatta
    10861 ttgtcattga tgaggaaatt gtaaaatatg gcgcttatct aagcgtagaa gaattagaaa
    10921 aattaactaa cattggaatg agtattcagg aggataatgg ggtacatgga acatagaatg
    10981 aatatgcaca gttgagaacg gaatttgaca tttatttaga ccaacagggt ataccctgtt
    11041 ggtctttttg tgcgttcatg atatacttgg tgtcattttt atatttaaat ggcacaaata
    11101 tttctaatta tttgtcattt aacgctgaaa atttttgcta tttatctaaa aaagtgacag
    11161 gatactcatt tggccttcat agaattttct aaatatgaat catcaatgaa tttaattaaa
    11221 aaaataagta gggcaaaata taaatgtttt tgccttactt gtttccatca gtttagtttt
    11281 aaattgataa gttatatagt tatggttgaa tgtgtgtctc gcatattagt atttttacca
    11341 tctgctagtt ttattattaa tctataacaa tagataattt ataattcgca tattcattaa
    11401 gctgttccaa aaagctgttc attgattttt ttgaagtaaa tctacattcc aaaagataac
    11461 agccccctcc actaatctta taatttctaa tcaagaattt ttcatgattt ttaat
//
LOCUS       DACEKH010000086        11340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEKH010000086 DACEKH010000000
VERSION     DACEKH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.090"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            19..936
                     /locus_tag="IW662_002154"
     CDS             19..936
                     /locus_tag="IW662_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0866969.1"
                     /translation="MPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKNLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="IW662_002155"
     CDS             939..3122
                     /locus_tag="IW662_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0866970.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="IW662_002156"
     CDS             3119..4120
                     /locus_tag="IW662_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0866971.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="IW662_002157"
     CDS             4135..5049
                     /locus_tag="IW662_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0866972.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..5410
                     /locus_tag="IW662_002158"
     CDS             5294..5410
                     /locus_tag="IW662_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0866973.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5426..7345
                     /gene="tet(M)"
                     /locus_tag="IW662_002159"
     CDS             5426..7345
                     /gene="tet(M)"
                     /locus_tag="IW662_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0866974.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7443..8816
                     /gene="tet(L)"
                     /locus_tag="IW662_002160"
     CDS             7443..8816
                     /gene="tet(L)"
                     /locus_tag="IW662_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0866975.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9380..10642
                     /locus_tag="IW662_002161"
     CDS             9380..10642
                     /locus_tag="IW662_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ0866976.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10887..>11340
                     /locus_tag="IW662_002162"
     CDS             10887..>11340
                     /locus_tag="IW662_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0866977.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKI"
BASE COUNT         3736 a         1896 c         2376 g         3332 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa aaccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgctc
     6061 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
     6121 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
     6181 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     6241 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     6361 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     6421 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     6481 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     6601 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     6661 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     6721 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     6901 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     6961 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     7021 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     7081 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     7141 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     7201 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     7261 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     7321 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     7381 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     7441 ttatgaagtg taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg
     7501 ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg
     7561 caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa
     7621 ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt
     7681 tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt
     7741 ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc
     7801 cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat
     7861 ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa
     7921 tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca
     7981 ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata
     8041 tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat
     8101 atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca
     8161 ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg
     8221 gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt
     8281 atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc
     8341 ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag
     8401 gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt
     8461 cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg
     8521 ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg
     8581 aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta
     8641 ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg
     8701 aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg
     8761 tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat ttctaaatcg
     8821 ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa caggaattca
     8881 aatctttttg ttccattaaa gggcgcgatt gctgaataaa agatacgaga gacctctctt
     8941 gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa
     9001 attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt
     9061 ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc
     9121 ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca
     9181 ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt
     9241 ggtcggcact gccgacagcc tcgcagagca cacactttat gaatataaag tatagtgtgt
     9301 tatactttac ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt tgtgccacct
     9361 aaaaaggagc gatttacata tgagttatgc agtttgtaga atgcaaaaag tgaaatcagc
     9421 tggactaaaa ggcatgcaat ttcataatca aagagagcga aaaagtagaa cgaatgatga
     9481 tattgaccat gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa atattgatta
     9541 caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa aaacgaggaa
     9601 agatgctgtt cttgtaaatg agttgctagt aacatctgac cgagattttt ttgagcaact
     9661 ggatccagga gaacaaaaac gattttttga ggaaagttat aaattatttt ccgaacgata
     9721 tggcaagcaa aatattgctt atgcaacagt tcataatgat gagcaaaccc ctcacatgca
     9781 tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt ttaatcgtca
     9841 agaactgtta tggctacaag ataaattccc cgagcatatg aaaaaacagg gttttgagtt
     9901 gaagcgtggt gaacgtggct ctgaccgtaa acatattgag acagctaaat ttaaaaaaca
     9961 aactttggaa aaagagattg attttctaga aaaaaattta gcagttaaaa aagatgaatg
    10021 gactgcttat agcgataaag ttaaatcaga tttagaagta ccagcgaaac gacacatgaa
    10081 aagtgttgaa gtgccaacgg gtgaaaagtc catgtttggt ttgggaaaag aaataatgaa
    10141 aacagaaaag aaaccaacca aaaatgttgt tatatcggag cgtgattata aaaacttagt
    10201 gactgctgcg agagataacg ataggttaaa acagcatgtt agaaatctca tgagtactga
    10261 tatggcgaga gaatataaaa aattaagtaa agaacatggg caagttaaag aaaaatatag
    10321 tggtcttgta gagcgattta atgaaaatgt aaatgattat aatgagttgc ttgaagaaaa
    10381 caagtcttta aagtctaaaa taagcgattt aaagcgtgat gtgagtttaa tctatgaaag
    10441 cactaaggaa ttccttaagg aacgtacaga cggcttaaaa gcctttaaaa acgtttttaa
    10501 ggggtttgta gacaaggtaa aggataaaac agcacaattc caagaaaaac acgatttaga
    10561 acctaaaaag aacgaatttg aactaactca taaccgagag gtaaaaaaag aacgaagtcg
    10621 agatcaggga atgagtttat aaaataaaaa aagcacctga aaaggtgtct ttttttgatg
    10681 gttttgaact tgttctttct tatcttgata tatatagaaa taacgtcatt tttattttag
    10741 ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta
    10801 aaattggttg cacagaaaaa ccccatctgt taaagttata agtgaccaaa caaataacta
    10861 aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa
    10921 aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga
    10981 aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa
    11041 gagtaaaaga ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa
    11101 agttgtatcg agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag
    11161 caatgaaaca tggcattcag tcacaaaagg ttgttgctga agttattaaa caaaagccaa
    11221 cagttcgttg gttgtttctc acattaacag ttaaaaatgt ttatgatggc gaagaattaa
    11281 ataagagttt gtcagatatg gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta
//
LOCUS       DACEKH010000087        11238 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEKH010000087 DACEKH010000000
VERSION     DACEKH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.091"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            83..1129
                     /gene="gmd"
                     /locus_tag="IW662_002163"
     CDS             83..1129
                     /gene="gmd"
                     /locus_tag="IW662_002163"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAQ0866978.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1209..2222
                     /locus_tag="IW662_002164"
     CDS             1209..2222
                     /locus_tag="IW662_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866979.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            2259..3203
                     /locus_tag="IW662_002165"
     CDS             2259..3203
                     /locus_tag="IW662_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAQ0866980.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWKNGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            3271..4584
                     /locus_tag="IW662_002166"
     CDS             3271..4584
                     /locus_tag="IW662_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ0866981.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVISASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            4668..5570
                     /locus_tag="IW662_002167"
     CDS             4668..5570
                     /locus_tag="IW662_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAQ0866982.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSVSFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            5575..7227
                     /locus_tag="IW662_002168"
     CDS             5575..7227
                     /locus_tag="IW662_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0866983.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEKVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKADSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            7251..8117
                     /locus_tag="IW662_002169"
     CDS             7251..8117
                     /locus_tag="IW662_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0866984.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(8159..8350)
                     /locus_tag="IW662_002170"
     CDS             complement(8159..8350)
                     /locus_tag="IW662_002170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866985.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCGKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            8380..8919
                     /locus_tag="IW662_002171"
     CDS             8380..8919
                     /locus_tag="IW662_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866986.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGADVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            9047..9400
                     /locus_tag="IW662_002172"
     CDS             9047..9400
                     /locus_tag="IW662_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866987.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            9867..10343
                     /locus_tag="IW662_002173"
                     /pseudo
     CDS             9867..10343
                     /locus_tag="IW662_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            10343..10537
                     /locus_tag="IW662_002174"
     CDS             10343..10537
                     /locus_tag="IW662_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866988.1"
                     /translation="MAETRPKGYPKLKEYTPNRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            10543..10828
                     /locus_tag="IW662_002175"
                     /pseudo
     CDS             10543..10828
                     /locus_tag="IW662_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            10842..11208
                     /locus_tag="IW662_002176"
                     /pseudo
     CDS             10842..11208
                     /locus_tag="IW662_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
BASE COUNT         3153 a         2303 c         2855 g         2927 t
ORIGIN      
        1 ttatcgtgta ttcttgaaaa gtattagaaa ttacgagcca tttatatggt cttatcactt
       61 acataggaaa ggataatgta tcatgaaaaa agcgctgatt actggtatta ctggtcagga
      121 cggttcctat ctggcagatt tcctgctaga gaagggctac gaagttcacg gtatcactcg
      181 ccgtgcttct atttccaata ccgctcgcat tgaccatttg atgggcaaga tcacccttca
      241 tgacggtgac ctgtccgact catcttctct gattcgtatc atcagcattg ttcagcctga
      301 tgaaatctat aacctcgctg cacagagcca tgttcaggtt tcttttgatg ttcctgagta
      361 ctccggcgat gttgatgctt tgggcgttat gcgtattctg gaagcttgcc gtattctggg
      421 cctgacgaag aagactaaga tttatcaggc atctacttct gagctgtatg gcaaggttga
      481 agaagttcct cagcgtgaaa ccactccgtt tcatccttac agcccttatg ctgttgcaaa
      541 gcagtatggc ttctggatca ctaaggaata tcgtgaagct tatggcatgt tcgctgtcaa
      601 cggtattctg ttcaaccatg aatccgaacg tcgcggtgag aatttcgtca cccgtaagat
      661 caccctcgct gccggccgca ttgcagaagg cctgcaggac catctggaac tgggcaacat
      721 ggactccctg cgtgactggg gctatgcaaa ggactatgtt gagtgcatgt ggatgattct
      781 ccagcatgag actcccgagg acttcgtgat cgctaccggc gagcagcaca ctgttcgtga
      841 cttcaccgag aaggcattcg cagcaaatgg catgaccatt cgttgggaag gcactggcat
      901 tgatgaaaag ggctacgatg ttgaaaccgg caagatgctc gtttgtgtaa atccacagtg
      961 gttccgtccg actgacgttg acaacctgtg gggcgacccg actaaggcaa agaccgttct
     1021 cggttggaat ccgcagaaga cctcttacga ggaactggtt aacatcatgg caaagcatga
     1081 ccgtgaattg gcaaagcgcg aagctgcaat gaagaacgca gtcaagtaag ctgacattga
     1141 ataaacatat ctgtttctat ctttccagtc acggcttcgg acatatcgcg cggaatctgc
     1201 ccattattat ggcaacactc tgccgatgcg aagcgaagat tacactggtt tgcggcgcaa
     1261 atcatctgga ttttgcaaag cgcaatttgg agcaggaact gcccaaagag ctgatagacc
     1321 gcgtgcgcta tcgtccaata aacacagata tcggtctgat taaccgtgag ggcaccctac
     1381 tggtggacac gggtaagctg accgagatct gtgaatattt tttggcaaag gttccttctt
     1441 tggcaaagac agaagccgaa tggctcaagc gagaagctgt cagcgcagtg ctttgcgata
     1501 tgccaatctg ggcaatggat gcctgcgata tggcaggagt acctttcctg tatgtaggaa
     1561 acttcacttg gacagagctg tatcgcgaat ttttacccga acatatttgg aaagaatatg
     1621 ccaagcgtta cggtaaaatt cagcacacca tgctttatgc gctaaataac gaagaaatgt
     1681 tggaatttct ccaaaacagc gaaaaaacac cgacttccgt tgtggcgcgt ccgattcata
     1741 acgacgaagt gcaacgcatt aaatcacagc atgaaaaacc gcctgtattt gtcgcactcg
     1801 gtatgtccgc gcggttcaaa cagccaattg atgtcagtca cgcgccgtat gatttcatta
     1861 caacgctagg tgttccgctt gtgggagaaa acgtaaccgt tatttctaac agcaccccca
     1921 atacacagga ctatatagcg gcggcagatt acgttattac aaaggctgga tggggaactg
     1981 ttgcggagtg tattctcgca cagcgtccga tggcactgtt cgcccgcgat acagttttgg
     2041 aagatagaac aactatttgt aaattgcaaa aactgggtct ggcaatttcc gtcacaccgg
     2101 aaaacctcca ccgcgtagag cgggtgattg aggacctcaa acagctagac acctcaaatt
     2161 ttgatacatt ttatgatgct gcgagcgaaa tcgcagacaa attgatttct ctcatgcaat
     2221 gagagatgct ctaaatttag aaaggagcaa gcattattat gatggaaaaa gacgcaaaaa
     2281 tctacgtagc cggtcatcgt ggcatggctg gttccgcaat cgtgcgcgag ctgaaccgtc
     2341 aggactacaa caatattatc acccgcaccc ataaggagct tgacctgtgc cgtcaggacg
     2401 ctgttgaagc atttttcgca caggaaaagc cggattatgt atttctcgct gctgcaaagg
     2461 ttggcggtat tatcgcaaac cagaacgctc tggcagactt catgtatgaa aacatgattc
     2521 tggaaatgaa cgtcatcaac tccgcatgga agaatggctg taagaagtta cagttcctcg
     2581 gttcttcctg tatctatccg cgtatggcac cgcagccgat gccggaaagc tgtctgctga
     2641 caagtgagct ggaaaagacc aacgaggctt atgcactggc aaagatttct ggcctgaagt
     2701 actgtgagtt tctaaacaag cagtacggta ctgactatat ctctgttatg cctactaacc
     2761 tgtatggacc taacgacaac tatcatccta ctcacagtca tgtgctcccc gctttgattc
     2821 gtcgcttcca tgaggctaag gaagcaggtc tgcctactgt tacctgctgg ggtgatggtt
     2881 ctcctctgcg cgaattccta tatgttgacg atctggcaaa cctgtgtgtg ttcctgatga
     2941 ataactatag cggtgacgag actgttaatg caggtactgg caaggaactg tctatcaagg
     3001 aactgaccga gatggtagca aaggttattg gttacgaagg cgagattctg tgggatacca
     3061 gcaagccgaa cggtacacct cgtaagctgc tggatgtttc caaggcaacc aagctgggat
     3121 ggacatataa gactgagttg gaagatggca tccgtctgtc ctacgaggac ttcctgaata
     3181 acccgatgcg tgctgaacgc taatttaccg aggttgctgt gatttttgag gattgcagca
     3241 gcctctttct tataaaaagg agagatcatt atgaatattg ttttactgtc tggcggttct
     3301 ggtaagcgcc tttggccgct gtctaatgac atccgttcca agcagttcat taagattttc
     3361 aaaactgaag atggatatga atccatggtt cagcgtgtct accgccagat taagaaggta
     3421 gataacgacg caactgttac gattgcaacc tctaagacac aggtttctgc tatccataac
     3481 caacttggtg aggatgtggg tgtatctgta gagccttgtc gccgcgatac cttcccggca
     3541 atcgcattgg ctaccgctta tctggtagat gtgatgggca ttgatccaga agagtccgta
     3601 gttgtttgcc cggttgaccc ttacgtagaa gatgactatt ttgaggccct gaaatctctg
     3661 tccgatcagg ctgacaaagg cgaagctaat ctggtgctca tgggtattga accgacttat
     3721 ccttccgaga agtacggcta catcattcct gagagcgcag agcagactgc tttcgtaaat
     3781 accttcaagg aaaaaccttc tgaggctgtg gcaaaggaat atatcgcaca gggtgcactg
     3841 tggaacggtg gtgtgtttgt ctacaagctg aagtatgtca tggagcgtgc acatcagctg
     3901 attgactttg tagattatca ggatctgttc aacaaatacg atactctgac taagatttcc
     3961 ttcgactatg ccgttgttga gcatgaggac aagattcagg tacagcgttt tgctggtatg
     4021 tggaaggatc tggggacttg gaatactctg accgaaggta tggaggaatc cattatcggt
     4081 aaaggtgtta tgaatgagaa ttgcgatggc gtacacatcc tgaacgaact ggatgttcct
     4141 gtgttggcta tgggtctgca tgatgtggtc atctctgcat ctcgggaagg tattctggtt
     4201 tccgataagg aacagtccag ctatattaag cctttcgttg acaagttcga gcagcagatc
     4261 atgttcgcag agaagtcttg gggtagttat aaggttatgg atgtagaagc agagagcatg
     4321 accattaagg ttactctgaa tgctagtcac tccatgaatt atcacagcca caagaaccgt
     4381 gacgaggtgt gggtggttct ggccggtact ggtaagacca tcgtggatgg atatgagcag
     4441 gttgttaagg ctggcgatgt gatcaccatg agtgctggct gtcggcatac cattatggca
     4501 gatactgagc tgaagctgat tgaagtacag cttggcaagg atatcaatgt taaagataaa
     4561 cagaaatttg agttagagta ttaaaaaacg gaggagggct cgttatacag aaaccgtttt
     4621 tattgaagcc ttccgcaaag gattatctgt gggggggaac cgcctgaatg atgaatttgc
     4681 aaaaggaatt gagatgtctc ctcttgcgga aacttggaat gctctacgca cccggatgga
     4741 ccgagctttg ttgtgtctgg tcagagtatc aggggatgac cttgacggag gtgttgcaag
     4801 aacacccaga gtttctggga acgcatccgc agaccaaagg agaattgccg atcctgatca
     4861 aattcattga tgcgaaaaag gatttgtctg ttcaggtaca tcccacagat gaatatgcaa
     4921 ttgaaaaaga aaacgggcag cttggaaaga cagagatgtg gtatgtactg gatgccagct
     4981 taatctatgg gctgcagaaa ataacagaag aagctgtgat ccgtgaagcg attgctcagg
     5041 gaactgttga gcgttatctt cagaaggttc cggtcaaaaa gaatgatgtg ttctttattg
     5101 aggcgggaac gattcatgcg attggtgcgg gtgcactaat tgcagaaatt caggaaaatt
     5161 ccaatctgac ttatcgtctc tacgattatg accgtgttga caaaaatggt aaaaagagag
     5221 aacttcatgt agacaaagcg ctagaagtag cgaacttgaa agggttgtca gaaccgaaac
     5281 agccgcttcg tgttctaaaa tatcgtgctg gtgtagctgc ggaattgctt tgcagatgca
     5341 agcatttcga ggtctctcgt atgattgtca acactgagag aagacagacg gtacactatc
     5401 gtgcggattc agtcagcttc cgtgtgttgc tgtgtgtcaa tggctgtggt accgttcgat
     5461 ttgataatga atcgttggac atctataagg gggactgcct ttttgtgccg gccgattcta
     5521 ctgaaatcag cattcacggg tcgattcagt ttctggatgt gagaggataa taatatgaat
     5581 tataaagtag aatatgagcg ttggctgaat catgccaatg cagatattat agaagaactg
     5641 cgttcctatt ctgagaagga cattgaggac tctttctata aaaatttgtc tttcggtact
     5701 ggtggtctgc gtggtaccat cggtgcaggc actaatcgca tgaacgtaca caccgtaggt
     5761 aaggcaagcc aaggtctggc agactatctg aataagaact atgaagccgc ttccgtggtg
     5821 gttggctatg attcccgtat caaatctgat gtgtttgcaa agactgccgc agacatcttt
     5881 gctgcaaatg gtattcaggt gcacttgtgg ccagaactgc ttcctgttcc tacagtatct
     5941 tttgcgactc gttatctgaa ggcagatgcc ggtgttatga ttacggcatc tcataaccct
     6001 tctaaatata acggctacaa ggtatacggt gcagacggct gtcagattac taccgaggct
     6061 gctactgcaa ttctggcaga aatcgaaaag ctggacatct ttgctgatgt aaagatggat
     6121 ggcgatgctt ctaagattgc atccattcag tcggaagttc tgactgcttt catcgaagaa
     6181 gtgaaggggc agtccgtcct gtttggcgaa aaagtggaca agaatgttgc tatcgtgtat
     6241 tctcctctga atggtaccgg tctggtgcct gttacccgca ccctggcaga gatgggatac
     6301 agcaatgtga ccgttgtgga agaacagcgt ctgccggacg gtaatttccc gacttgtcct
     6361 tatccgaacc ctgaaattaa ggaagctatg tctctgggta tggagtatgc caagaagtac
     6421 aatgcggatc tgctgttggc cactgaccct gactgcgacc gtgttggtat cgcagttaag
     6481 agcgcacatg gtgactatgt tctgttgtct ggtaatgaga ctggtctgct acttctggac
     6541 tacatctgtt ctcagagaat taagcatgga aaaatgcctg ctgaccctgt gatggtcaag
     6601 accatcgtca ccatggattt gggtgaacgt atcgcatcca actatggtgt tcgtaccatc
     6661 gatgtgctga ccggctttaa gtttatcggt gagcagattg gtatgttgga aaaggctggt
     6721 aaggctgaca gctatatctt cggctttgag gaatcttacg gctatctgtc cggtagctat
     6781 gtccgtgaca aggacggtgt taacgctgct tatctaatct gtgaaatgtt ctcttactac
     6841 aagactcagg gtatcagcct gctggataag ctgaatgaga tttatacaaa gtatggctat
     6901 tgtttgaaca ccctgcattc cttcgagttt gacggtgcgg caggttttac caagatgcag
     6961 gaaattatgg ttgaattcca caagggtctc gatataattg gtggtaagaa ggttattgaa
     7021 actttagact acagtaaggg cctgaatggt ctgccgaagt ctgatgtttt gaagtttatg
     7081 ctggaagata actgctctgt tgtggttcgt ccttctggta ctgagccgaa gctgaagacc
     7141 tatatcagcg tcagcgcaga gaacaaggaa gccgccgaag ctatggaagc tattatggct
     7201 gctgaactgg cggaaatgtt cgagtaagaa aagaaaggag aaatcttgta atggaggaac
     7261 tgttttctaa cgctttgaaa tcggtgaagt caaatcgtgt gctgtacacg gcatcgttct
     7321 ttgcaagatc ctctcttttg tatttgcagg aaattggaca gctgactgct ctgaaggcac
     7381 atacatctag gcgtacagga ttgtcatctt acctttgttt cctggtagaa gttggttcag
     7441 gcacattgga gtataagggg cagaaatata cgcttcaagt aggagatatg gtgtttattg
     7501 actgtttgaa gccgtatgtc catgagtcat cagaagatga tttgtggact ttgcggtggt
     7561 gccattttaa tggcattggt atgacggaag tctacaaaaa ataccttgaa cgaggtggtc
     7621 aaccggtctt taaaacatca aaaacagata agtatgctga cttgctgagg agccttcata
     7681 tggtggcaga gggacaggag tatgtccggg atatgaaaat caatgttcta ctcagtgaac
     7741 tgctgatgca tttgatggaa gattcttgga atcctgacaa agtgccgaat ttctctacaa
     7801 aaaaaatgga attgcagaac ataaaacaat atctggatga ccattacacg gagaagatta
     7861 cgctggatca gttgagtgca gagtttttta tcaataaaca gtacttagct aagatttttc
     7921 gtgaaactta cggtacaact gttgtcggtt atctgaatca gaaacgaatc acacaggcca
     7981 agcacttgct acgctttaca aatctaagta tggaagagat tgcaacaaaa attggcataa
     8041 cagatgcaaa ctatttttcc agaacttttt tcaaggtgga aggtgttcgt cccagcgaat
     8101 tccgcaagat gtggtaataa aaagccggcg gtgttgagcc gccggtcagt acctgtcgtt
     8161 aacccgcatt caaagcttta atctcagctt tgatttcagc acgaacacga tagtaatctt
     8221 cagtttcgat ttcggtatcc agccgagttg ctgtctttcc gcagtcagga caacgctctg
     8281 gctgttcgtc ggcagggaag ctgtagtggc aggaatcaca ggtgtaaatg tgatcgtcac
     8341 gtataatcat cacctaccaa tccagtgctt agatttttca tgcaattact attatatcga
     8401 attggtgatt ccacaaaaat ccgcttcttg ataaatgtac gattcagagt gatatatagt
     8461 actgtactgc caaagggaat accacaacgg cagaaatgga tgattcacat ggaagaaatt
     8521 atgcagatga ttttagggaa cgcaaaattc cagctcggac aggtggtttc gacccctgct
     8581 gtattggaaa ctttcaaccc cctgcagacc gcaaacggca ttcccgaagc cttgagcaac
     8641 ctttggttta gacaggaaat tagtattgcg gagatcgagg acgcagttct ggatgccaag
     8701 aacggcaagg agctagcaaa cagcctcaac cgcatcaagc ttttccgcaa gttcaccctc
     8761 gaccgagagc tggacggcat ggtcagactg aagggcgcgg acgtctggaa gaacatcagc
     8821 tacctcaagg tctacaccgc cacgccgcct aagaaaccga cccccccaag gttccagtcg
     8881 aacggctgga gccttttctt ctctcataaa tcggcataat gtatacaaat acagctccaa
     8941 atgattgtgt agtatatgcc tgtggattga cttgatatag tcgagagact atggcaatat
     9001 acacacaacc aaaggggaaa atcccaaaaa caaggaggaa acgctaatgg caatgagcga
     9061 aaaggcaaag aaatatttga aggagatcaa gggtgcaaag accatcccgg agctgaagga
     9121 tgtggagatc gcaatcaagc gagacggtat ccttgcttgg gcagagttca ccaagttgaa
     9181 cgaggcggtg gaagagaaga aggtcgccct tcgcaagaaa aagcaggaga ccagccttca
     9241 ggagatcctt ttctgggcct acaaaaagga atcagacaag ctgctgaaga tgatggacga
     9301 gggagcagac aaggatgcca tccagatgca ggtccagcgt tacgacagca tcggccagat
     9361 cattgcagag gcagacctcg aagatgagta tgaggtttga gaatacagcc tgaaccacta
     9421 agaaccaaat accgaaaggg acttctgaaa cagacagagg tccctttctt ttgctcactt
     9481 tggcggtcaa ttatgaacca aaaaccagtc gaatgattgt gtacattatg gctttcaaac
     9541 gacttgataa taatcacttt tagagtgata tatgtactac aaaaaacaaa agactggaga
     9601 aaacaacaga tgagcgaata cagaatcatt tatatcagcg acgaggacgg cggcgaggtg
     9661 gaaggatgtg gctacacggc tacgatggtt atgatgcggt cagaaacttc ttggaatttg
     9721 cctgcggcat cttgaaaatc ctgcgggtgc gattcatcag aagagtggac tgaaatacaa
     9781 gttagcagat agagaagaga gtcaggtttc gacttggctc tgttctctgt ctactggcag
     9841 aaatacattg aagggaggtg acgactgtgg caaccagagg aagaaaatca accccgacag
     9901 ccatgaagaa gctggaggga aatccgggca atcgcccact caatgcaaaa gagtcaacgc
     9961 cggaaaagaa ggcaccgacc tgtccgaagt ggctggaacc agaggcaaag aaggaatgga
    10021 agcgtctggc aaagcagatg gaggccatcg gaatcttgac agatgtggat atggctgcct
    10081 ttgccggtta gtgccaagcc tacgcccgct ggaaggaagc cgaggaattc attacccagc
    10141 acggtatcat cgtgaaaacc ctatccggct attggcagca ggtaccacag gtgtttattg
    10201 cccagaccta tctgaaaatt atgaaccgat tttccgagca gttcggtctg acgccttcgt
    10261 ccagaagccg tatcattgcc ggtgatggcg gtggtgtcgg agcagaagat gcgatggagg
    10321 ctctgttggg aggtggtagc tgatggcaga aaccagacca aagggctatc ccaaattaaa
    10381 ggaatacaca ccaaaccgat tcatgctttc ggaatgtcac tacgacaaag ccagagcgga
    10441 tcgggcggtg aactttatcg gacagctccg acacaccaaa ggcaaatggg caggcaaccg
    10501 cttctggctc ttgccttggc aggaacagat catctagtgt aatctcgctt aactgtatta
    10561 acggacgtag cgactttatt aagagtgttc atggtaggag cattagttgc aacaatgatt
    10621 gatattaaat ttttatggta ccaacgattg gcgatgtgac attgaatatg caaaactata
    10681 tcgatatgat attatctaaa atcttactcg ctgggatcgt cgaggtgttt attggttgtt
    10741 aggaaagaaa aatatgaccc aactcgagcg atttttatcg tattacttgt gtgtggcttt
    10801 atctgcattg tgtgtgattt cgaaataagg gagagccaaa aatgagtaga aaattgatat
    10861 tgattagtca cggaaaattt tgtgaggaat tgaaaaaaag tacaaaaatg attatgggac
    10921 ctcaagagaa tatctacacg ttacctttat taccagaaga tggcactgaa acatacagac
    10981 aaaagctctt actgtcatag attcgttaaa tgattttgtg gtactatgcg atcttttagg
    11041 cggtacacct actaatatca tttctaaact gataatggaa ggacaacaga ttaaacttta
    11101 tgtcggatta atcttcctat ggtcattgaa tttatcaact cgcaagtgat tggttcagag
    11161 cccgagtttg ttcgtgcagg atgtgaaagc attgtatctg tcaactaatt aattagtgga
    11221 atagtagaag atgaagag
//
LOCUS       DACEKH010000088        10958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEKH010000088 DACEKH010000000
VERSION     DACEKH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.092"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            90..1349
                     /gene="lysA"
                     /locus_tag="IW662_002177"
     CDS             90..1349
                     /gene="lysA"
                     /locus_tag="IW662_002177"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ0866989.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1336..2136
                     /gene="dapF"
                     /locus_tag="IW662_002178"
     CDS             1336..2136
                     /gene="dapF"
                     /locus_tag="IW662_002178"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ0866990.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2139..3020
                     /locus_tag="IW662_002179"
     CDS             2139..3020
                     /locus_tag="IW662_002179"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ0866991.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3017..3733
                     /locus_tag="IW662_002180"
     CDS             3017..3733
                     /locus_tag="IW662_002180"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ0866992.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3739..4434
                     /gene="dapD"
                     /locus_tag="IW662_002181"
     CDS             3739..4434
                     /gene="dapD"
                     /locus_tag="IW662_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ0866993.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4450..5451
                     /locus_tag="IW662_002182"
     CDS             4450..5451
                     /locus_tag="IW662_002182"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ0866994.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5448..6809
                     /locus_tag="IW662_002183"
     CDS             5448..6809
                     /locus_tag="IW662_002183"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ0866995.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6769..7920
                     /locus_tag="IW662_002184"
     CDS             6769..7920
                     /locus_tag="IW662_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ0866996.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7895..9064
                     /locus_tag="IW662_002185"
     CDS             7895..9064
                     /locus_tag="IW662_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0866997.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9239..10618
                     /locus_tag="IW662_002186"
     CDS             9239..10618
                     /locus_tag="IW662_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0866998.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3639 a         1913 c         2456 g         2950 t
ORIGIN      
        1 tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac
       61 ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag
      121 caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga
      181 gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc
      241 tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat
      301 tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta
      361 gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg
      421 aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat
      481 taagccaacg ctatttaaaa aaaatttgta tcatgcttcg gttgaatgtc ggagtagaag
      541 cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg
      601 aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag
      661 aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa
      721 tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg
      781 gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa
      841 aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat
      901 tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg
      961 gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg atgacagata
     1021 atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg
     1081 aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa
     1141 ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg
     1201 gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt
     1261 ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa
     1321 ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta
     1381 ttagattacg cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa
     1441 aaatatgata tagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac
     1501 aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac
     1561 gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca
     1621 agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca
     1681 gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata
     1741 gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt
     1801 gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat
     1861 catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa
     1921 ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc
     1981 tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt
     2041 cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca
     2101 gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg
     2161 cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga
     2221 tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat
     2281 cttctacgct cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg
     2341 ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa
     2401 aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca
     2461 acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga
     2521 ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat
     2581 tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg
     2641 cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg
     2701 atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga
     2761 atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc
     2821 aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca
     2881 atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc
     2941 tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag
     3001 aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt
     3061 cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga
     3121 agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga
     3181 taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac
     3241 gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt
     3301 gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc
     3361 agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa
     3421 agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct
     3481 gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag
     3541 tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat
     3601 atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc
     3661 agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga
     3721 caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct
     3781 aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt
     3841 aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg
     3901 gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac
     3961 agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt
     4021 atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat
     4081 attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga
     4141 gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa
     4201 ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg
     4261 attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta
     4321 gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt
     4381 gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga
     4441 tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa
     4501 acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct
     4561 tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa
     4621 ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta
     4681 tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca
     4741 gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat
     4801 acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt
     4861 aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt
     4921 tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca
     4981 gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa
     5041 aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag
     5101 cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc
     5161 tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc
     5221 cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt
     5281 cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag
     5341 aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc
     5401 gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc
     5461 tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc
     5521 gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac
     5581 gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc
     5641 atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag
     5701 atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg
     5761 aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac
     5821 tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact
     5881 atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat
     5941 atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat
     6001 tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa
     6061 gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc
     6121 atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct tatatggggg
     6181 ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa
     6241 taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa
     6301 aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc actttattca
     6361 aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac
     6421 atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag
     6481 cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg
     6541 tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata
     6601 aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca
     6661 catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc
     6721 aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa
     6781 tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa
     6841 tatttatatg aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg cgaaacagga
     6901 ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat
     6961 gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg
     7021 catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact
     7081 aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt
     7141 tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca
     7201 atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga
     7261 cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt
     7321 gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt
     7381 tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc
     7441 caaggtggaa cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt
     7501 tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag
     7561 tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac
     7621 catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca
     7681 gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt
     7741 ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt
     7801 ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa
     7861 atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag
     7921 tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc
     7981 gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt
     8041 caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg
     8101 attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta
     8161 tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt
     8221 gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata
     8281 tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg
     8341 gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa
     8401 agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt
     8461 gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata
     8521 tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac
     8581 aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat
     8641 ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc
     8701 cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag
     8761 cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc
     8821 tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc
     8881 tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc
     8941 gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc
     9001 tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca
     9061 gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag
     9121 acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta
     9181 atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat
     9241 ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga
     9301 actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat
     9361 ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga
     9421 tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca
     9481 cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa
     9541 cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa
     9601 tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc
     9661 aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc
     9721 gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt
     9781 gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt
     9841 agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt
     9901 tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct
     9961 agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt
    10021 agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta
    10081 tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc
    10141 tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt
    10201 accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca
    10261 ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt
    10321 gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa
    10381 agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt
    10441 atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact
    10501 gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg
    10561 gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga
    10621 aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag
    10681 ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    10741 cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc
    10801 aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact
    10861 tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta
    10921 aaaatccaga tcaagagatt gaagcggaaa tgcttaaa
//
LOCUS       DACEKH010000089        10798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEKH010000089 DACEKH010000000
VERSION     DACEKH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.093"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            90..308
                     /locus_tag="IW662_002187"
     CDS             90..308
                     /locus_tag="IW662_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0866999.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(597..1109)
                     /locus_tag="IW662_002188"
     CDS             complement(597..1109)
                     /locus_tag="IW662_002188"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ0867000.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1123..3420)
                     /gene="recJ"
                     /locus_tag="IW662_002189"
     CDS             complement(1123..3420)
                     /gene="recJ"
                     /locus_tag="IW662_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ0867001.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3562..3996)
                     /locus_tag="IW662_002190"
     CDS             complement(3562..3996)
                     /locus_tag="IW662_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ0867002.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4006..4794)
                     /locus_tag="IW662_002191"
     CDS             complement(4006..4794)
                     /locus_tag="IW662_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0867003.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4791..5732)
                     /locus_tag="IW662_002192"
     CDS             complement(4791..5732)
                     /locus_tag="IW662_002192"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ0867004.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5909..6496)
                     /locus_tag="IW662_002193"
     CDS             complement(5909..6496)
                     /locus_tag="IW662_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ0867005.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6717..8030)
                     /gene="obgE"
                     /locus_tag="IW662_002194"
     CDS             complement(6717..8030)
                     /gene="obgE"
                     /locus_tag="IW662_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ0867006.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8318..9328
                     /gene="gap"
                     /locus_tag="IW662_002195"
     CDS             8318..9328
                     /gene="gap"
                     /locus_tag="IW662_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0867007.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9413..10348)
                     /locus_tag="IW662_002196"
     CDS             complement(9413..10348)
                     /locus_tag="IW662_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0867008.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            10534..>10798
                     /locus_tag="IW662_002197"
     CDS             10534..>10798
                     /locus_tag="IW662_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0867009.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGW"
BASE COUNT         3036 a         2413 c         1873 g         3476 t
ORIGIN      
        1 tttatcactt ccctctttac aaaccgaaca aatattcgta tactaaatat acgaacaatt
       61 gttcgcataa attaaagagg tgagaattca tgaaatcaat gagacgtctt ggcttagtat
      121 taactgtttt gtttttaggt attattattg gtaatttcgg catacgtcca gctttacttg
      181 tgtttgttcc gttatttact ttgtggttca tgttgtggga tgaaaagaag taccgtcaat
      241 ctgagcggcg cagaattcat gaagatcatt tctatcatga gccctactac agatccaggc
      301 aatcataata aggattgact gctaattatt caaaagacaa gaagttaaac ttttacgggg
      361 gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa agccagtttt
      421 tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg tctttgcttg
      481 tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg acttttgagg
      541 gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt ttctgcttaa
      601 tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg gagatccatc
      661 aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat cagttcgatc
      721 gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac acgttgacct
      781 ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa tccataagtc
      841 acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa accgactcct
      901 aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc aactaccata
      961 tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg ataagcgtca
     1021 ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg atagtcggga
     1081 atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg ttaaccattt
     1141 ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt tattgagccg
     1201 tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga ctttttgtaa
     1261 aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca taaaaattaa
     1321 taatttttca gggatacgca aatattgagc tattgctttt attttatgac gtatatcaat
     1381 tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg tgcctacccc
     1441 attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga atgaataaga
     1501 ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa ctagagagtt
     1561 tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa caatcgcagc
     1621 ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta aataaaggct
     1681 accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa atacttgcag
     1741 accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt tccattcatt
     1801 gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt cttgagtacc
     1861 aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa atttcagatg
     1921 ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa ataaaaactg
     1981 aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa ttcgctctac
     2041 tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc tcatcaagtc
     2101 aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga cattttcact
     2161 tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata aatctcgcat
     2221 ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc ctttagctga
     2281 tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca ttattttccc
     2341 agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat ggatatgatt
     2401 ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg cttttctttc
     2461 ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat caaacgtcgc
     2521 aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta gtctgggagc
     2581 aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc tttcttcaaa
     2641 tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta atgaaacaag
     2701 gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg ctactaaatc
     2761 caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg ctactccgac
     2821 tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa caatcgcata
     2881 agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt ctacaccagc
     2941 ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag tgatgatcaa
     3001 ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat acccatgctc
     3061 aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg tttctttcat
     3121 taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga ttttttctcc
     3181 actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta tcagatacgg
     3241 atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt cctcatggta
     3301 acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac ggatcatttg
     3361 gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg cttgtctcac
     3421 tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa gcagaaagaa
     3481 agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat cttttgtacg
     3541 accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg gcgattgagt
     3601 tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt gtaactccgc
     3661 ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct gttcttcttt
     3721 tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct gagtcagctg
     3781 tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata caatcagtgc
     3841 accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg aagtgaagcc
     3901 aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa tcaaaactaa
     3961 aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact ttttgttaaa
     4021 aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt ttgatgcaac
     4081 ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc ggacgatcat
     4141 ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat agcttccgct
     4201 tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg tcgtaacagc
     4261 tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga ctgcaaattt
     4321 cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag aggcgatatt
     4381 aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg tcaaaaccat
     4441 cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga atgtaaggaa
     4501 gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc cgacttcttc
     4561 catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta ccttgaaagc
     4621 ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta caaatgccat
     4681 tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac agatttgtgc
     4741 gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat tcatgcctct
     4801 tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt cgtgttttca
     4861 aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata tctcgcactg
     4921 atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc agcttgatga
     4981 gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa tgtgctttca
     5041 tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt tctcgtatcg
     5101 cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt tccattgatc
     5161 gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta cagtggtcca
     5221 gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg atcatgttca
     5281 accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata aacagaaaat
     5341 tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag cggataactt
     5401 aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc ttttggcccg
     5461 taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag cccgggaagt
     5521 ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt tctcggacga
     5581 atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa ccaaacttca
     5641 ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt tgcaggaaca
     5701 cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt taagttcctc
     5761 tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg gttcaaatta
     5821 aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa cagcagcgaa
     5881 cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt tcattgtcgt
     5941 tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc gcatgatttt
     6001 cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag cgagtaattt
     6061 ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct tcatcagcaa
     6121 tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg aacacggtct
     6181 gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg gcaaaccatc
     6241 ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt aatccacccg
     6301 tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat tgatgtgtcc
     6361 cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga acagcatgta
     6421 actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa tttaaaatga
     6481 ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta ttttaccttt
     6541 aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg ctgtgacaaa
     6601 agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat cacttctgtc
     6661 acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt gcttgcctat
     6721 tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt cgcaccgcgt
     6781 gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg catcactgtt
     6841 tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc agataagatc
     6901 cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt gaatccatag
     6961 tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt tacttctaac
     7021 aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc agaaattgga
     7081 aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg ttctttgaat
     7141 ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat gatttgcgga
     7201 cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag atagtcttca
     7261 taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac tcgtgttctt
     7321 tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc ttcaatcaag
     7381 cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat tcctagatta
     7441 ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga aataacggat
     7501 agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc caatactttc
     7561 aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat ttccggtgca
     7621 ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc ttttgccaca
     7681 actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc cgcatctcgg
     7741 acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc gtgcatccct
     7801 ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg aaaatccatc
     7861 aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc accgtctccg
     7921 ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat tccgtctccg
     7981 cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat aattaagtcc
     8041 tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa aagtcttact
     8101 tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg tttaccttct
     8161 tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt ataatttttt
     8221 gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat tgaggatgcc
     8281 gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag taggtattaa
     8341 cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct cagatgatat
     8401 tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat tattgcaatt
     8461 tgactctact catggtactt atcctggcac agttactgca acagaagact caatcgtagt
     8521 ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc cttgggcaaa
     8581 agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg aagaaaaagc
     8641 aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag ctggtgctat
     8701 gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca aaatcatctc
     8761 agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa ataatgaatt
     8821 tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac aaatgctctt
     8881 agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg acaatactat
     8941 cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc tacaaggaaa
     9001 attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg aattggtttc
     9061 agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa aacacacagt
     9121 tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg tcattggaac
     9181 aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg gtgatttcca
     9241 acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc aaatgattcg
     9301 cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa gaagcctttg
     9361 actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc gttcaatgaa
     9421 catattggct taaaaattgt tggactactt gctgatattt ttcaggatct gtcttgtatg
     9481 cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc gttgcacgat
     9541 agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt ataaagagca
     9601 ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa aagtagccag
     9661 cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga agactaaaca
     9721 tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa tcttcaatga
     9781 ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt gctgctccca
     9841 tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc actttgttta
     9901 tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc ccttcgcttt
     9961 ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga aacatctttg
    10021 cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga gctgtttttt
    10081 cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct tttgaagtga
    10141 tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc gtttctttgg
    10201 atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa ggcacaacag
    10261 cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct aaagcgacgc
    10321 ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct tttttcatat
    10381 aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt tataacccgc
    10441 gaattgtaaa attaagttag aaaaataaaa aggcatttat ggtacactca aattgtattt
    10501 ccgacgaaag aaaacaaagg agtgtaacca taaatgacct acaaacatct taccatagac
    10561 gaactgacaa tgatagaatc atattatctt caacataata agccggttga aatcgctaac
    10621 cgaatgggac gtgctataca aactatttat aatgtagtca ataagttcaa gcaaggcaag
    10681 actgctcttg attattggca ccagtataaa gaaaataaga aaaaatgtgg tagaaaagtc
    10741 attcaattac ctgctcatga agtagattac attaaagaga aagtcactct tggttgga
//
LOCUS       DACEKH010000090        10779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEKH010000090 DACEKH010000000
VERSION     DACEKH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.094"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            91..1137
                     /locus_tag="IW662_002198"
     CDS             91..1137
                     /locus_tag="IW662_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0867010.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1486..2118
                     /locus_tag="IW662_002199"
     CDS             1486..2118
                     /locus_tag="IW662_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ0867011.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2706..4145
                     /locus_tag="IW662_002200"
     CDS             2706..4145
                     /locus_tag="IW662_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0867012.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4440..4658
                     /locus_tag="IW662_002201"
                     /pseudo
     CDS             4440..4658
                     /locus_tag="IW662_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4907..5122
                     /locus_tag="IW662_002202"
     CDS             4907..5122
                     /locus_tag="IW662_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867013.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5339..5595)
                     /locus_tag="IW662_002203"
                     /pseudo
     CDS             complement(5339..5595)
                     /locus_tag="IW662_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5782..6693)
                     /locus_tag="IW662_002204"
     CDS             complement(5782..6693)
                     /locus_tag="IW662_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0867014.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6712..7716)
                     /locus_tag="IW662_002205"
     CDS             complement(6712..7716)
                     /locus_tag="IW662_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0867015.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7713..9836)
                     /locus_tag="IW662_002206"
     CDS             complement(7713..9836)
                     /locus_tag="IW662_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0867016.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9841..10761)
                     /locus_tag="IW662_002207"
     CDS             complement(9841..10761)
                     /locus_tag="IW662_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0867017.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3297 a         2036 c         2019 g         3427 t
ORIGIN      
        1 tcaacaactt cgtcaagtga aagtagtacg atttcaagta ccgaatcttc aacaacttca
       61 agtagtacaa gtgaaataac ttcttcgaaa gtgacaaaac caaaccatat agaatcttca
      121 aaaaaggaag cgccaaaaac ggaaggacaa acaaaaactt tttcgacacc acagcaaata
      181 gaatcaacag aatctttcca atcacaacca atactatctg gtaatgaaga aattcatttt
      241 gacaaaaatg aatctgtaga aaattttatt atgaaaattg gtgagtctgc gagaaaaatt
      301 ggtcagaaat atgatttata tgcttctgtt atgattgcac aagcgatctt agaatctgcc
      361 agtggtcaaa gccagttagc tcaggcaccg aattacaatt tatttgggat aaaaggaact
      421 tataatggaa actttgtcat tatggtaaca aacgaagatt tgggtaatgg aacgctttat
      481 acgacccaat caaaatttag agtgtatgaa aactatgagg aaagctttga agattatgcc
      541 aaattattaa ctaagggaat tagtggaaat aaagattttt atgcaggagc attaaaagct
      601 aatagtaaaa catatcgaga agcaactaaa ttccttacag gacggtatgc aacagataca
      661 caatactatt tgaagctaaa tgaattaatt aaaacctacg atttaaccaa ctatgataaa
      721 gaagttaaag gtccacaaat gagtaagact ggataccaag tgcctattca taattattca
      781 ataaccagtt tgtttggaaa tcgtggaaat gaatttcacc gaggacttga tatggcagct
      841 acacagggtg agccgattca tgcagttaaa gctggtaagg ttgttaaagc agagtttcac
      901 tactcttggg gaaattacgt ggtgatacaa cacgaagatg gatcaaccgc gttatatgct
      961 caccagcaac aatacctagt aaaaactgga gataatgttt tgcaaggaca aataattggc
     1021 tatgtaggaa gtactgggaa tagtactggc ccacaccttc atttggaaat ttgcaaagat
     1081 agtagtcttt ctcagagcat gttagttgat ccgaaaacct tattattcgg agactaaaaa
     1141 aggtaatcaa tctgacaaag taacaagaaa atggttggat tcttagacct ttgatcagga
     1201 tattgagact ggaattgata tctcgattac gatgggtttg acaaataaaa caagtccatt
     1261 atcgaatatc gagtgtatta ttgccgtctt tatgctcata ttttgagcag gtttcactag
     1321 atgaaaacaa tttatctatc ttgaggattt cgtgtacgta tcgttcagtc ttgtattgta
     1381 aattagtcat aaaatcagac taagaaaaat tggaaatatt tttttctgat ttattaaaag
     1441 ttaattgaaa ttatatggta cttctttctt gggagggtag gtgcagtgaa ggaaagatat
     1501 ggtcacctta ctgttgttga ggagattact cataatggat ataaaaaata tcgttgccta
     1561 tgtgactgtg ggaatgaaac gattgtatat cgatcaaact tactatcagg tagaaccaaa
     1621 agttgtggat gtggaaatta taaaaatagg cataaatata aaaatttgac aaataaaact
     1681 tttggtaagt tagttgcaat caagccaact aaactgcgaa gtgaaggaac tgttgtttgg
     1741 gaatgtcaat gtctgtgtgg gagaaaaaag ttagtatcgg caaagcgact tctaaaaggt
     1801 gatgtaaaaa gctgtggatg tttgaaatca aaaaagcatc acattgaaaa taaaaaattc
     1861 ggattactta ctgcaaagta tcctttggac aataactgtt catggaaaac aaaatgggtt
     1921 tgtcattgtg agtgtggcag tatgtgtgag ataagttata gtaacttatt ttttggacat
     1981 actcaaagtt gtggatgttt aaaaaggcaa gaaacaagga catgtgttga agggacagta
     2041 atagagtgtc taacgtcaaa attaccgaaa aataatacaa gtggtgttaa gggagtttat
     2101 cgttctaaag agaaatagat tgcgtatata actttaaaaa aaaagaggta ctatttagga
     2161 tcgtatgatt ctattaagag tgcagctcaa gctagaaaag atgctgaaaa taaattgttt
     2221 caacctttga ttgacaaata tcgaggtaat cattaattat tgtggataat aattaacagt
     2281 gatatataaa catctataat atattattca tactattgat atttagtata atcctatgga
     2341 attgaatatc atagcaatgt atttgaaaag caaattggaa aacaaatagg tgcaacacca
     2401 taaatatgaa aaaattggga aaaagtagaa tggaaatttg cttacgtctc acgagatagc
     2461 tcacactatt aatctgaaga acaatgaagt cgacttttag aaattgctag aagaaagtaa
     2521 agcgaattat cactagttac tatagagtaa gttcaaataa agcatctcca acttacatgg
     2581 ttacatgagg atatcaaatc gaaattactc aagcttccta tggctagagt cgcgagaagt
     2641 tgttgggcag tagacatagg ttatattgtt cttagctaca cgatcattag aaaggaaatc
     2701 ctattatgta tagttatcaa gaagatgcaa aagtccaaaa aaatcgctgg tggattttga
     2761 ttgctatatc tatgtttacc ttcatgtcca cattggatgg cagtattgtc aatattgcac
     2821 tacctaccat ctcagatgat atgggcatac caatgaatca agcggaatgg gtagtgtccg
     2881 tttatctgat gattgtctgt tcttgtttgt tgctgtttgg taaaattggc gatagccgtg
     2941 ggaaaatcaa agtatttcgg attggtacgc tgatctttgt cttgggatcg ctgttgtgtg
     3001 ggttcaatca ttcgctaggt attttattgt tcgcacgggt ggtacaagcc attggtgcta
     3061 gtatgactat ggctacaaat gccggaatca tcacggaagt ctttcccata catgaacgtg
     3121 gtcgggcatt gggagcaata ggtgcgtttg tttcattagg ctcgattgct ggaccaggtc
     3181 ttggcggctt gattttgtcc caattttctt gggcgtacat tttttggatc aatgtaccgg
     3241 tgggaatcgt aacgatgttg attgctgaaa aattcttacc aaaagatatc accatgagcg
     3301 gccatacaat cgatggaggg ggctttgctg ccattgcggc tacgattatc agcttttttg
     3361 gagcaatctt tttggggcaa gaaagtggct ttttccatcc agtccccctt gtactgttcg
     3421 taatttcagt gattgccttt gtcagctttt taaaagttga aaagaaagca gcctctccat
     3481 tgatttcttt cggtattttt aagaacaaga tttttaccat gagcttgatt acagccttgt
     3541 tgattttttc ttccaacttc tttgtcaatg tagtgattcc tttttatctg caaaatgctc
     3601 gcgggttgag tcctagctat gctgggctgt tgatgatggt ctttcctttg atgatggtcg
     3661 tcggggcgcc tttaagcggc tacctgaccg accgggttgg cccggagatt ttggtattag
     3721 taggcttggg cttattgtcg gtaacccagg tcatgtatat gtttttgtca gtggactctc
     3781 ctttgtggct atacattgtc gctactgcca tcatgggtgc cggcaatgcc ttgttccagt
     3841 cccccaataa tacgatggtt atgagctcgg tcactatcga aaacttggga ctggcaggct
     3901 cgatgaattc ctttgcccga aatttgggga tggtcagcgg gatttcgctt tctacgacgg
     3961 tcttatatca agcgatgtct gctaagatgg gccagcaagt aactaactat atcgcaaatc
     4021 ggccggatgt ctttttatat ggaatgaaga ttaccttttt aggcagcttt gttttgtgtt
     4081 ttgttgcctt tttcctgact atctggcgta tcaaaaaaca aggaggtatt cagaaaaatg
     4141 tctgaaaaaa ccactgttga agaatttttg gaggcctgcg tatgggtgag tgggatttcc
     4201 atggatgatg gaagatgaag tgaacttcta aaagccgaaa aactcaagta ttatcttcaa
     4261 aatactagtg ctcaatgaga tgcttgattt gatcacaaga agactgcgaa agccgctggc
     4321 aaccgcaagc taggtatccc accgatggac tacgttttag aggctactgg ttatgaatac
     4381 ggagccaacg ctctcatctg cataagaata catcacccag attatgtaaa gattttcgat
     4441 acttctgtca aaaaatcatt aagatagata aatgatttcc gtctagtcaa attggttcag
     4501 aatgtagaca cacagatggt aagaagtcac tcacaattcg agaatggact tgtcctattt
     4561 gccgtataca tcataatcga aacgtcaacg atagtatcaa tattttgacc aagggctatg
     4621 aatacaagca ttgccttaga aaacatcaag aaccttagga acaacgggga tagcttggtt
     4681 aataagagat accgctgttt aggcttgcaa gtatactctt ttatcaagaa ttccgtggct
     4741 ttagtcatga gaggcttaat actatgaaaa ttttctttag catttttgat ttaggttcct
     4801 tttctaatta taataagatg accactacag taacctactg aaatatataa acagccactg
     4861 tagtaccatt ttcaaagtag tctatgagag gagaatcttc attcttatga acgctggaat
     4921 tgcggatatg aatttaataa aaaaaaccct taatgatttt acttccaatt caatatcaaa
     4981 aggtactggt atcaatttaa gcacaataaa aaagctgaaa agtggtgaaa gatcagtgga
     5041 aaagctgaat cttttagatg ctataaaaat aacggaattt gctatgaaaa atggaaaagc
     5101 tgagattgaa atatggagat aattatcgtc tagtgaatta cttgccactt agctgaattt
     5161 aacgatcctt ggtgcttgaa gggggagatt cttgggaata gagcatactt gcaagcctat
     5221 gtctttacta acaacgatat ctcttattaa ccaagctatc cccgtagttc taaggctttt
     5281 acttttatct aagcaataat cgtattcaaa ggaaattttt gacccttaga aagtctcatt
     5341 ttatcagacg tgagcttagg cattgctgaa ttttgttaat tcggttttag gttgtgcttt
     5401 gagcataata tgaaagtatt ctttatcacg atttcattca tctaaggtaa tgtgatgtga
     5461 ttctgaaatt ctttcaaagg cagtttttgc ataagctgac attacatcat caatgacatg
     5521 tttacaatgt ttcactacta aaataagggg gtaaccaaga agaaaaactg aatgattatt
     5581 tgtatctaat tccatggtat atcaaccatt tcctatatag actgattatg acatggatta
     5641 aaataaaaag gcactatgtc ttaattgtcg gttttcgagt taccgaatgg cattcatcac
     5701 ccacctatag aggatgggcg acttctgcct gaaataagtt acactactta taacttctaa
     5761 taaaattaga ttgagtcatt attatttaac atcaacgatt ttccagtttc catttttttg
     5821 taatactaat ttgtactgaa agtagctagt ggtttttgat acattatcaa ggtattttat
     5881 ctttgtactc actagaaaat tttttccgtt ttgtagataa actggattct ctaactccga
     5941 aaactttaag tcaatattga ttggtcgcat cgcaccattt tcaacataat actccaattc
     6001 tttttcagaa gctgatggat aaagtttaaa aaatgtttct aaaaaatcgg tgatttcttc
     6061 cgttgttttg gcatcaattt ctgaatcgtt ttctacttgt tttgcttcat attttgcctt
     6121 gtctggcttt ccaatcattg ttggcaaact agttactaaa tgattcctgt tttcatcttc
     6181 aaagattgtc actcgatatg cagaagaaat tgattgcgtc ttttttccct ctgtaatatg
     6241 ttgctccact aaaaatgaaa cgacaaattc ttccgagtgt ttttcaacat ctagaatttt
     6301 tacagattga acttcggaac tcgtgggaat atctgctcga accatatctt ggattaaagc
     6361 gagaccgttt tctgtaaggt actgttcgag attactcatt ctcttttcaa ttgcttcttt
     6421 gttatttttc catgaaaaat attctttcgc aaaatcctct gtaaaacttt caacgccaga
     6481 agtattcact accttctctt ttataaccac tttttcatga gttgtatgtt gatcaatggc
     6541 ggtgaaattt ttataaacgc caaaagcaag actacaagac aataaaagcc aaagggcaag
     6601 caccattttt cgatgcttac ccacggatac aactcgttct tttttctttt ttgggctctt
     6661 ttccttttga ttccgttcaa tttttatctt cattcttttt cctcttttct cttatttgta
     6721 tcggccagct ccgataagat gctgccgcca atagttttcc gttagattcg tccaccccac
     6781 tggatctcct gcatgaaaca tgcgattctc ccctgcgtaa atgcccacat gggtcacata
     6841 agtaccggca tcataggttg aatgaaagaa aactaaatca cctggtttgg catcttttag
     6901 atcaatatga gtcatggcat catattgatc ctgagccacc cgaggaagtt tcaggtcaac
     6961 tttggcataa caccattggg tcaagccact acaatcaaac cccgtttctg gacttgagcc
     7021 accaaacaca taggccgttc cttcatactt aaatgcctca tcaaaaattt cttggactat
     7081 tttgtcatca aactgagttg tagttaggta ttgcttcact aataaacagt aaaacatatt
     7141 gccataatta taccgccacc caccattttc cttaatcgcg attgggttgg ggtaatcgac
     7201 tttttgacca cctgattttt cctttgaaaa agattcagct aaagcaaaac tatgcttctt
     7261 cccgtttttc ccgacatgat ctagataccc gccaccatag ttataggcct gaataatact
     7321 atcttgatca caccctagtt ctttggaacg cttaaccaaa gacgcaaaat agagacagcc
     7381 ttgtttgatg gatgcctcgg tacttaatga attaggtggt aatccggctg actcagaact
     7441 ttgcataacg tcttccattg tgccaccaga ttccacttga ataatcgcca aaagaattgg
     7501 aatatactct gttatatccg attctttggc atacttttcc accattgcac ggtgtttcaa
     7561 tacttcttct gagacactag ttccgccagg agaagtgcca gaactaccat ttccctcact
     7621 gtcttcacca aacaacaaac ccacacacat caaaataccc ataaaagaga tggtcagaaa
     7681 agttaatgaa ataacaccca ctttcttcaa gttcattttt tctcacctga ccttgatttt
     7741 ggacgatggt tcattctttt ttgtactttt tgattggtct gctttggcag agtggacctc
     7801 tgtttcactt gttggaaaga atttttctta cttgctgatt ggcttttccc ttttgaaatg
     7861 atttcttgac tagttagttt tcgtttgatt tcgggattca gatttgctct cacaatccgc
     7921 tttggtgctt cttttttcag catcatatct ttctgaggtt gagctgcttt atctattgga
     7981 atattcgccc ttctttttcc ctgacgattt gttgcttcac tcagtatctt gcgtttttcg
     8041 tccattttcg catttcttcg ttgtcgttgt ttttctcgca aatccactcg atccgctttt
     8101 tcttggaaca gtccgcgctt gaattcttct ggtgcttttt tcgacttttc gattccttta
     8161 tggagattgt atttcaaatt cgttggcgta ttccttacct gatcctttac ttgtttagtc
     8221 ttatctttca ccaaagcttg ggtatccaaa actttgccga tttttctccc agccaaattc
     8281 attcgggatt gtttcttatt atttgaaact ggattctcct tagttaagtc agatagtatt
     8341 tcgtgatcgt tcggtaatcg tgaattcttt cgtagagaag aaccggatgg cttaaatttg
     8401 gattttgatt gttcctttgc caacttattt ccgactgctg caccaccacc aaaagccaaa
     8461 gctcgtccca catttcgatt gagttgccgc aattttcgat tcattaacat ttgcggtttt
     8521 cgcatgacgc gtcttcccat tgactggcta tcattactct ggaggttgaa catactcata
     8581 atgtcgccca atttgaaata gataccggca aaggtcacaa tttgcagaaa agcaaccatg
     8641 aaaaatggat aggttgccga aatattgaaa aacatcgtgg agatgctaaa tgcagtcgta
     8701 atcaccaaag taacacccgc tcgcatcata atcgtattga ataaacgaat aatcgccttc
     8761 tttcctaaac tttcataagt cggcaacatg gacaataaga aactaattgg cagaaacatc
     8821 gcaaagataa tgaagagaat ttgcgagaac aacataatcc ctgttagaag aaaaacaaag
     8881 aaggaaatgc caatattgaa caataccaaa aagaccacgg tccctaaacg gttcatggtc
     8941 ttggtaatac tgagattcgc attgtcattg tcttcaatct ctgccttgac cgcttcttcc
     9001 cgatctttgc ctttgttttc atctggactc acagatagaa tcttgttgac ccggtcttca
     9061 ccaatttctt ctatgttgga atcatcaaat tgcagtagta accaaggttg ttggacttga
     9121 atcgaaaaca aactatcccg aatcaaatcc acactatctt ttccttggct ttctgaattg
     9181 ggtacaacga tctcggttcc aagggtaagg gccgcttcac taacatctga gctaaagtca
     9241 ttgatttttg tgatataggt aggcgcataa gcaatgaacg aacccgatag tagaaaaatc
     9301 atcacaaaat taatcacggc tcgaactgct ttcgtggttt ctcgttttaa taatccggta
     9361 tacgccacat acacccccag agctaaaatc atcaataata gaaacccaaa ataaaatccg
     9421 gaggtctgga gtccattttc agtaatacca gccaatgttt ggatattctt tccaatactt
     9481 tcagccgtat ccgaaatgaa atctaatttg taagcctctt gaaccacata acccgtggcg
     9541 ttcgacaaat acaaactcac cgtccaaatg aaattagtaa tggcataaag accatacatc
     9601 acgcttttcc ctaagccatc accccaattc caaggcaacc aatcccaaga gctatccaca
     9661 aagaaatcta gttgatagtt gttcagtgaa tatttggaat atagatttcc tgcttcaact
     9721 gtatcatcaa ctagccctgt cgcctcggct accgtaccaa ctaaactcaa tagaactatc
     9781 agagtgagta tgctaataaa aacgattccc acaatccgaa aaagtttctt tttcatcccc
     9841 tcactccatt ctctcttgaa caggtggacg ggtatcaaag gcatggaaga gttcttcaaa
     9901 gactggatgt acctgaacca cgcctacccg accatacaaa tcctgcatca agcattggcc
     9961 attttccaaa tctcgtaggc gtttttgatt gctttcatct tctcgatcca agccaaagaa
    10021 ttccaaggtt ttcttgattt catgaatatc ggtactgcga aacgcaaact tcaacccgat
    10081 attattcttc aagcgttcat ccagtaaatc atcactattt tgcgtgacaa agtaaacccc
    10141 ggcattcata gcccgcccag cacgaacgag cttattcgat aatgttttcc cttgcgctac
    10201 ttgaagaaaa ctccacgcct catctaagtc tacaattttg aagatttctc gattactatg
    10261 gataaagtcc aaggcaaaag tggaaatgac gattagcatg gaaacactaa gcaattccat
    10321 ggtggtatat tcttcaaaag tggtttctgc atctggtaaa accaaatccg ctacttgaat
    10381 gatatttaac tgcctatcta aactaatcga ctgggtaatc gaaccatctg agaaaagcaa
    10441 atgggcaaaa tcataatcgg taaaggactc gatgtgatcc gcgatatttt ccgcaactgg
    10501 tgagccttct tttcgaagct cattaattac ttgtaataat ccccgttgat tgctttgggt
    10561 aaccgtccga atggccttac gcaacactgg aaacttctcc ccatctcgac tagaaattcc
    10621 tgttaaaaag gtcagaatat caattgctag actttcagaa tccttctttt tctccataat
    10681 cacataagga tcaagtaatc cttgattttt tggcttacta gttaagttga gaatattgat
    10741 ttcatccgca atttctggca aggtttcttt ccagccacc
//
LOCUS       DACEKH010000091        10420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEKH010000091 DACEKH010000000
VERSION     DACEKH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.095"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            504..770
                     /locus_tag="IW662_002208"
     CDS             504..770
                     /locus_tag="IW662_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0867018.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            914..2905
                     /locus_tag="IW662_002209"
     CDS             914..2905
                     /locus_tag="IW662_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ0867019.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            3259..4560
                     /locus_tag="IW662_002210"
     CDS             3259..4560
                     /locus_tag="IW662_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0867020.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(4690..6060)
                     /locus_tag="IW662_002211"
     CDS             complement(4690..6060)
                     /locus_tag="IW662_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ0867021.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(6242..6565)
                     /locus_tag="IW662_002212"
     CDS             complement(6242..6565)
                     /locus_tag="IW662_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0867022.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(6891..7496)
                     /locus_tag="IW662_002213"
     CDS             complement(6891..7496)
                     /locus_tag="IW662_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ0867023.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            7826..8542
                     /locus_tag="IW662_002214"
     CDS             7826..8542
                     /locus_tag="IW662_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ0867024.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            8555..9250
                     /locus_tag="IW662_002215"
     CDS             8555..9250
                     /locus_tag="IW662_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ0867025.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHAELYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            9334..9963
                     /gene="udk"
                     /locus_tag="IW662_002216"
     CDS             9334..9963
                     /gene="udk"
                     /locus_tag="IW662_002216"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ0867026.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT         3315 a         1893 c         2157 g         3055 t
ORIGIN      
        1 tcataggaat aatgtacttg tgatcgtgaa ggtgacaggg ggacaataat tattcgaaaa
       61 aaggggactg tgacaaaagc cgtgtcacag tccttttttt tcgaataaac ggcgttcaaa
      121 agcttgcatc aagtaataag tccagccaag ttaaaaacat taggagctgt ttttacttgg
      181 ctgttcttat tacttgatgc agaacagctt ttttacaacc tctccaataa acgatgttca
      241 atcagctgtc cctcgatatt agctcaaaaa aaacggcaaa atatgaggaa ctatttttcc
      301 gtttgtttct ctaatactca ggtcaaaccg ctgatttcac aacctctgat tcacggtgtt
      361 ccagaagcag cttctacgga aataagctga acacttttgt tacaacttct ttttttctgg
      421 agtttcttgg cgcatatttt tggagtcctt ctgaataaag cgtaaaataa aagtgagaaa
      481 tatggataaa ggagggaaag gagatgctga attttttatg gtcgttattt atcggcggta
      541 tattaggttt gatagctggc gcaattttag gaaaggaagt cccgggaggc gtatctggaa
      601 atatcattat cggtttttta ggtagttggc tcggcgaatt tttactagga ccactgggac
      661 cggaactcgg cggtttttat ttgataccag cattattggg tgcaattttt tgccttgctc
      721 tttattcatt tgtggcaaga cgtatcagag aacgtccaaa gaaagaataa ttttattcaa
      781 ataagttgac acttcagtgt gttacttgga taattagaag caatttagga aagcgggtgt
      841 catggataag aatatcatct atcattcaag cttacctaaa ggatatccat ataacaagaa
      901 atgagggaaa aacgtggcaa caacgcacaa taccaaaaag atatcaatgg ctggcctgct
      961 tgtagcgatg ggagtcgtct acggagacat cgggacaagt ccgctttacg ttatgaaatc
     1021 gattgttgaa gataatggcg gattgtcagg aatcaatcct gattttgtta tcggatcagt
     1081 ctcattgatt ttttggacat tgactctact gacaacaatc aaatatgtcg tgattgcatt
     1141 gaatgcagat aaccatggtg aaggtggaat tttctcactc tacacacttg tccgaaaagg
     1201 tgggaaatat ttgatcattc ctgcaatgat cggaggagct gccttactag cagatggggt
     1261 attgacccct gctgtgacgg taacgactgc gattgaaggc ttgcgaggaa ttccgcagtt
     1321 ttttgaccgg ttcggtaata accagaacgt gatcgtcatt atcactttag ttattatttt
     1381 tgttttattc atggtccaac gctttggtac agatattgtc ggaaaagcat tcggaccaat
     1441 catgttcgct tggtttactt ttttaggcgt aatgggatta atgaacttca gtcaagactg
     1501 gacagttatc cgtgcactga atccttatta tgctatccag ctgcttttta gtgcagataa
     1561 caaattaggc ttgttcattc ttggaaatat tttcctggct acgacaggtg cagaggcgct
     1621 ttattccgat cttggacatg tcggcaaacg aaatattcgt ctgagctggc cttacgtcaa
     1681 aatttgtttg gttatcaatt attttgggca ggctgcgtgg ttgttgaatg tttatcaaaa
     1741 ccctgaagca cagcaaatca agaacctgaa tccattcttc caaatgatgc ctcaaagttg
     1801 gatcgtgatc ggcgttgctt ttgcaacagt cgctgcagtc attgcttcgc aagcattgat
     1861 tacaggttcg tttacgctag tttcagaagc aatcaagttg aaactgctgc ctcggttaaa
     1921 gatcatgtat cctgggaaca gtatcgggca aatgtatatc ccagcagtca atctgatttt
     1981 atggatcgcc tgttctctag tggttgtcac attccgaacg tctcatcata tggaagctgc
     2041 ttacggtcta tcgatcactg tgacgatgtt gatgacaaca gcactgcttt acttttatct
     2101 tatccaaaat gggcattctc gttggcttgc atatttagtt acctttttct tcggtgcgat
     2161 agaagtcgtt ttctttatct caagtgttgt caaattcttc cgaggcggtt ttgtagccgt
     2221 tctgattggt ttgatcatct tagtcgtgat gtttatttgg gaacaaggga acattattcg
     2281 cgaatcgtca accgaggatg tgtctttgag agattatatc ccgcagctta gagaattacg
     2341 tgatgatact tctttaccaa tctatcagac aaatgtggtc ttcttggttc ctgatatggc
     2401 tgaagataaa gttggccgac agttcatcta ttcgatttta gataaacggc ctaaaagagc
     2461 acgagtctat tggtttgttc atgtggaagt gacggatgag ccatatacga aagaatatca
     2521 agtagatatg atggacactg attttattgt ccaagtgaat ctgttcttag gtttcagggt
     2581 ccaacaggaa atcaacgtat atgttcgcca agtcatccat gatttgatga aacaaggccg
     2641 tttgccaaaa cagccacaaa gatactctct gacaccaggg cgagaagttg gcgatttcca
     2701 atttgtcctg attgatgaag tagtttccaa tgtgacaact ctaggaaaat ggcaacgtca
     2761 aatcatgcag gcaaaactgg caatcaaaaa aatcgctacc tcgccagaaa catggtttgg
     2821 gctagagtac agcgaagtaa aacatgaaac tgttccgctt atcattggaa atactcggaa
     2881 aacatggttg aaagagcggt tgtgaaagta gcgctttgac ctgaattaat gtcgaatgtc
     2941 agctacttga acaccgttta tcagaggtca tgaaaaagct gtactgcaac aagcagtaag
     3001 aacgagcaga taaaaacaac ttctcatgtt tttcatctgt ttgggcttaa tgccgcagat
     3061 gcaagctttt gaataccgtt tttatcagag gtcatgaaaa agctgtttag caacaagtaa
     3121 cgcaatgatt ttgaagtgat aaggagatgt ggctgaaaaa tgccgcatct ccttttttat
     3181 tcctaaatat gaatacgcat tattttcaat ctttttgtag aaaaagggta caatagaaaa
     3241 agagaaaggc gggagaatat ggttatcaat ttcattattt tatttttgat gattgcaatt
     3301 acttcatttt ttgtagcaag tgaatttgct ttagtgaaaa tcagacgatc aagattggaa
     3361 caactagaaa aggaaaatgt aaagaacgcc aagctagctc ttcatgttac acaccattta
     3421 gataactatt tatcagctag tcaattagga attacgctaa ccggtttgat catcgggtgg
     3481 gtcggtgaag gatctgtcgc ggctttacta gaaccagtga tcggtaaact accttttagt
     3541 tcggctatta gcagtacaat ctctgtggcg ctgggattta ttttagttac gtatattgat
     3601 gtggttattg gcgaactgct tcctaagagc tatagtatcg tcaatacgga gaaggtcgtc
     3661 ttgttcgtag ttaaaccact tcattatttt tataaagtaa tgtttccttt tatttgggta
     3721 ttgaatcatt cagctgcagg gctagggaag ctattaggtg tgcgtctcgt atctgaagga
     3781 gaagagacat tgtctcaaga agagctgaca ttagtcgcat tgaattctta tgaaaaagga
     3841 gagttgacca aagaagaata tcattatttg gaaaacgtgt ttgaatttga tgataccttg
     3901 gcaaaagata tacaagtaga ccgtacgtcc atgcaagtat tcgaagcttc agaaaccgtc
     3961 aaagaagcga taaaagcttc gattgaacaa ggacatactc gctatccggt tatcttagaa
     4021 tcaaaagaca atgttttagg ttatgtcacc ttgccagatt tgatcaagca atcttataaa
     4081 gatgaccagc tgacagtaga acagttgatt gaagagccga tcgtaacaac tgaaacgatc
     4141 cctataaaaa aattattgac gatcatgcga aaaaagggaa agcatattgc tatcttaaaa
     4201 gatgaatacg gagggacaag cggcttagtg acgattgaag atattttaga agaaatcgtc
     4261 ggcgaaatcc gagatgagac agatttggac gaagcattga tagcggaaca gtcagatggc
     4321 tcttatatca tctccggcaa actaacatta gatgatttcc agcgttattt tcatgtagag
     4381 attccagagt ttgaagaaac gaattttact acacttgcgg gctttgcttc tagccgatat
     4441 aaagaaataa aagcaggaac aatcattgag attgcatcat tccgtttcac ggtattagaa
     4501 taccagcatg cacatatcga ttatttcaaa gtagagtcca cggaaagaaa aacagaataa
     4561 aagaaaaaag gctgtgacat tttttgtcac agcctttttg ttatctaaaa aacctgtttt
     4621 cggtaaacga agaagatatt ttcaataaaa gttttcattt ttagccgcaa cgatttcttt
     4681 taatcaaggc tatcttgctt ctgcatgttc taatgtgaaa tgttcctctt cacccatgtg
     4741 tttcaattgt ttcatcgaac gcatataaga gtaaagcaac acggcaacag aaccgatcca
     4801 agctaaagga gaagctgctg ctgctcctgg gtatccccac attcctgtaa gaacgatagc
     4861 tgcaaaagaa cgcataagca attccatcat accggcgatc gtagggatct tgctttgtcc
     4921 aagcccttga agtgtgtaac gtaaaatgaa caggactgct aagatccagt aaagagaccc
     4981 attgatattg aagtaggttt gtgctaattc aaatacacgt gtttctgatg ggctaacaaa
     5041 caattgaaca aagaaatgac cgaagaaaat gacgatagca cctgctaaga gactaaatcc
     5101 gacactcatc aataaagttt gacgtacccc ttgcaagatt cggccatatt ctttcgcacc
     5161 atagttttgt gcagtgaaag ttgccattgc aataccaaat gacatcatcg gctgatttgc
     5221 aaattggtcg atacgaccag cagcggcttg agcagctacg acatctgtcc ctagactatt
     5281 tagtgctgct tgtaacacga tagcaccaac agcgatgatc gaagattgaa acgccattgg
     5341 caaagcagcg tttaggtgta cagcaaactc ttctttttca aatttgaaat cttttttgcg
     5401 taactgtaac aaaggcattt ttttcttgat atacactaga cacagtacgc tggaagaaac
     5461 ttgtgcgatt actgtagcaa tagctgcacc ttccacaccc atgccaaaat agataataaa
     5521 gatcaaatca agtacgacgt tgatgatgac cgcaatgatc agaaagaata gtggtgtgcg
     5581 gctgtcacca agtgcgcgaa tgacattaga aagcaaattg aaactgactg atgcaaagat
     5641 ccctaataaa ataattgaaa taaatgtttg tgcttgatca agtatttcag gaggagtttg
     5701 catcaattga agcatcggac gaataaacag gagacttagg acagttaaaa tcacagtgac
     5761 gacaagacta atgacaacac tagcagcaaa acttttcttc agaccccgat aatcttttgc
     5821 accgtaacgt tgtgcggtaa taatggctaa accagctgtt aaaccttggg caaagccaat
     5881 gatcaaaaat gtcaggcttc cagttgcacc aaccgctgcc agcgcatttt tacctagcgt
     5941 ctgacccacg atgatcatat cgatcatatt gtagaactgc tgaaaaacat ttccgatcag
     6001 taaaggaaca gtgaacatca aaatcagttt agcggggctt ccttttgtta aatcgcgcat
     6061 cttttaaact ccttatatct ttaaaattta gttagtagta tatagcgttt atcctgagaa
     6121 taccaattag aaatcaatga taatttatga aactccataa aaagaagcca agaattgagt
     6181 caaagaatca acaacttggc ctggtctgta aaataaggga catccacgtt tatctatcac
     6241 cttaatattg tctgatctca cttggtaaaa ggatcgtttc tctcgtaccg tcatcatagg
     6301 cgtatacttg agcaggaaat tggttcgaat aggcaaacgg caaatcaata tccatttcca
     6361 cattgcttcc tgcttgagaa aaatgcatcg tcacttttcc acgattttcg atcaaatcaa
     6421 aggttaatat attttctaat ggaatgactc ccttaagatc taggtcaata ataaaccata
     6481 tactgtcaat caattcatct ggcaaacttg atactacacc aactgaagcg taacggttgc
     6541 ggctgctatc aaaactttca aacatctgtt taacctcctt cgaatcaacg aatgttttat
     6601 ttttataatt aatcctagga taaactagaa atacttactt caacgagggt tcattctaat
     6661 aaagaatcag gaaaaatgca acaataattg aatacttttt ttcaaagtat aaatttttat
     6721 aaaggtacga taaaagtatg cgaggaaaag cttggtaaat taggattttt cgtttgcaaa
     6781 gctatttgta aaagtggaag cctcatatga aaataaacga ttagaaagaa aaaaaggcgt
     6841 gttaaaagct gctgacccgc cgcttctaac accacctgat tgtttcagta ttattcttct
     6901 gttgaaccga ttacttctgg ttcgccacct tcacctgtgc tttcttcttc actttgagcg
     6961 actacagcag cgatttgctc ttcactgtcg gtcacgaatt tgtaatcgtc gttttttgga
     7021 agatcggcta ctgtaataga atcacctaat tccaagtttg tcacgtcaac ttcaattcgt
     7081 tcaggcaatt tgtctggtgt agcagatacg actactgtat acaagttttg agctaacact
     7141 ccaccagatt tagcacccac tgattcgcca acaagaacga cttctgtttc gacttctgtt
     7201 tcttccgaca tgtttacaga tagaaattct gcatgttcga aatgattggt aaaagtgttc
     7261 gtttggatct tgtgaaccaa tgtattgact ttttgtccat cgatgtccat cgtgatgact
     7321 gtgttcgctc catgctcacg caacaatttg cttagtgtca aaccatcgaa tgagataggg
     7381 gtactttcta ctttataccc ataaacaaca gctggaactt tcccttcgtg acgaagtttg
     7441 tttcttaatg aacggggacg tacttctctt ttacttactt ctaatgatac tgacataacc
     7501 aaaattcctc cttaaaatgt gactgtctgc tttggtaacg gacagatttt atttaagtga
     7561 attctaaagg ctaatctcgt gcacatcatc gaaggcaggg cgcttaaaaa agcgcaaaag
     7621 gagagatgcg cgcatctccc cttagaaagt caggattcaa cttttctcca ccaggtcatt
     7681 acgctgtgta tgagaaatcc aattcacttt actatttaac cgtaacgtga actgttaatt
     7741 aagtcaagga ataactatga tgatgtattt gcaagcaaga atattcaagg tataattaat
     7801 tagtagtaat aagaaaggaa acagcatgcg tttagataaa ttaatagaag aaaaattaga
     7861 aacgacacgc aaggaaatga agcggctgtt tgctttgaag caagtgatga tagatggcac
     7921 agtcgaattt cgacaaaatc gaaatgtaga tagtttactg catcaaatcg aagtagcagg
     7981 tgaacactta gaaacaaatg agtcctatta tcttctcaat aaacctgaaa aagctgttac
     8041 agctgtatca gatccagaaa agacgacagt aatcgatctg attgcaagtg ccgatcgttt
     8101 ccaaccatta tatccagtgg gaagactaga tagagataca acaggactat tgctgatcac
     8161 ttcgaatgga caattgggat atgaaatgct acttccagaa aaaaaggttt caaagactta
     8221 tcaagtagtt gtgaacgaaa gggtgacacc ggcagacgta acagcatttg aaaatggaat
     8281 cgtattcgct gatgggtatc aatgtaagcc ggctgattta aaaatactca aagcagacaa
     8341 gcaccaaagt acggttttat tgacgatcca agaaggaaaa ttccatcaag tcaaaaagat
     8401 gttcttagcc caaggaaaga aagtgaccgc attgaaacgg ctaagcatgg gaccattgga
     8461 attagatgaa cagctaggag taggcgagta tcgatcgttg acattagcag agatgaaaaa
     8521 gttaagtatt tattttaaat aggaggtcaa aaagatgaaa tataaaactt tattgttcga
     8581 tgtagatgat acattgttag attttcaatt aactgaagcc aatgctttaa gtgctttgtt
     8641 tgcagaacaa ggaatcacat tgacaccaga aatcgaaacc agctataagg ctttgaatca
     8701 tcatttatgg caggagtttg aaaaaggcag actatcacga gaagaaataa taggcagtcg
     8761 ttttgggtta cttttccagc aatttgggaa aacagtagat agtgatcaaa tggaaaaacg
     8821 ctaccgccac tacttgaacc aaggtcatga tcttgttcca ggaagtctag aaatcctgaa
     8881 aaaggtccat ccgcatgctg aactttatat tgtgacgaac ggtgtgtcgc acacgcaaca
     8941 tcggcgatta acagatgcag gaatgcttga ttacttcaag gatatctttg tctcagaagc
     9001 tgtcggagcg caaaagccga tgaaagaatt ttttgatcat gtcttttccc acattcctaa
     9061 ttttgataaa aagcaaactg tgatcattgg tgattctttg acttctgata tcaaaggagg
     9121 aaatatggca gggatcgata cgatttggtt caatagaaat aaattaccag aaatcgaaga
     9181 aatccagcct acttatcgaa tcgattcatt agaggaactt tattcattat tggaaattga
     9241 aaaggattaa ttttcagaaa aatatagcat tttcgtgttt tttcgctata atagattaga
     9301 atgaaaaaac ggatacgaaa gaggttttag tcaatgacaa aaagcaagcc aattatcatt
     9361 ggtgtgacgg gtggatccgg aagcggaaag acaagtgtca gccgtgcgat tttcaacaac
     9421 ttcccggacc attccatcat gatgcttgaa caagattctt attacaagga tcagagccat
     9481 ttgagttttg aagaacgatt gaacacaaat tacgatcacc catttgcctt tgataatgat
     9541 ctgctgattc agcatgtagg cgacttactg aactataaag ccattgaaaa acccgtgtat
     9601 gactatgtag cgcatacaag aagtcaggca acgatcattc aggaaccaaa agaagtgatc
     9661 attctagaag gaatcctgat tttagaagac gaacgtctgc gtgacttgat ggatatcaaa
     9721 gtatacgtag atacagatga tgacattcgt atcattcgtc gtatcaaacg ggatatggaa
     9781 gaacggggac gcacattgga ctccgtgatc gaacaatatt tgacagttgt taaaccgatg
     9841 tatcatcaat ttatcgaacc aacgaagcgc tatgcggata tcattgttcc agaaggcgga
     9901 gaaaaccacg ttgctattga tttgattaca acaaaagtag ctagtttctt aaaccataaa
     9961 tagtgcgcag cgaaacagtt gaacggactg cgaccgtttg aaaagcaagg attctgcttt
    10021 ttgaacagtc accagccaac tgttttttta tttataacaa gaggttgtga aaacagcatc
    10081 ttgacctgag tattaaaaga agaaacggaa aatagctgtt tatattttac cgttttttga
    10141 gctaataccg aaggtcagct gattgagcac cgtgaagtag aggttgtaaa aaagctgtct
    10201 ttgcattaag cgataagagc atccaaataa aacagctcct cacgtttttc gttgggttgg
    10261 acttattatc gtagatgcaa gcttttgaac accgtttaac gagaggttgt aacagaagtg
    10321 gtcagatcca ataaataaga agaaacttac gaaaattatt tctcgaattt gtgtgagatt
    10381 tcgacttatt tttttgaaga agctcttttg tcgcaccccc
//
LOCUS       DACEKH010000092        10037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEKH010000092 DACEKH010000000
VERSION     DACEKH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.096"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..524)
                     /locus_tag="IW662_002217"
     CDS             complement(<1..524)
                     /locus_tag="IW662_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0867027.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVK"
     gene            complement(691..2811)
                     /locus_tag="IW662_002218"
     CDS             complement(691..2811)
                     /locus_tag="IW662_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0867028.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            3041..4219
                     /locus_tag="IW662_002219"
     CDS             3041..4219
                     /locus_tag="IW662_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ0867029.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            4235..4993
                     /gene="aroD"
                     /locus_tag="IW662_002220"
     CDS             4235..4993
                     /gene="aroD"
                     /locus_tag="IW662_002220"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ0867030.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            5016..5501
                     /gene="ybaK"
                     /locus_tag="IW662_002221"
     CDS             5016..5501
                     /gene="ybaK"
                     /locus_tag="IW662_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ0867031.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5572..6825)
                     /locus_tag="IW662_002222"
     CDS             complement(5572..6825)
                     /locus_tag="IW662_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0867032.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6942..8291
                     /gene="gdhA"
                     /locus_tag="IW662_002223"
     CDS             6942..8291
                     /gene="gdhA"
                     /locus_tag="IW662_002223"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0867033.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            8403..9749
                     /locus_tag="IW662_002224"
     CDS             8403..9749
                     /locus_tag="IW662_002224"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0867034.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
BASE COUNT         3207 a         1810 c         2083 g         2937 t
ORIGIN      
        1 tttttcaccg atttgaattc atcgaaacac accacttctg gtaacggctg gttcaaagaa
       61 acggttttag gccgaaggct gcgaagtatt cgaatgacag tggtcggaga aatgtgcttc
      121 atacgtgcaa tcaaagacat agaaataggc tcacttaata gctcaagaat ggcttgtttg
      181 actctgcggg caatggagca gcgacgatcg ctaactgaat cttctgctaa aaaagtcttt
      241 ccacagtgtt tacatttgaa gcgctgtttg ttaatccgca caatggtctt gtagttactg
      301 acatcattca ataagatgga tacttttttc cagccccatt taatgatcgt ttgcttattt
      361 tgatagtggc aatggaagca ttcctctggt tgataggtca atgtgccatc aaaaacaaaa
      421 catgtttcgt cattaatgcg ctctttgcga aaggcatctt catgaaaggt aagatttagg
      481 tctagaatat ctagtaactc tttggtaaac tctgaatagg acatcttgaa acactccttt
      541 gaatggtttt cgtcgacttt attctaaagg atttcaaggt gtctgtttgc gtttattaga
      601 aaaaagaaaa agtattggca acagaaggcg tcaccaacac tagatattat agatcctaat
      661 tttgtcgctg ctccttttaa atttatttta tcattcggaa gaacttgtgg tcgttccgtg
      721 aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccgt tgtgttcact
      781 aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt tctctaagcc
      841 attcttgtag tcataatcac tggtatcaat tgggtctaat ccactattcg tatagaaccg
      901 aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa ctttttcttt
      961 cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt caccagtttc
     1021 gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat caccatcacg
     1081 gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact gaatataatt
     1141 ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt gtccgccgta
     1201 agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag ggacacgctg
     1261 catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta attcagctag
     1321 atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga tgactgaatt
     1381 atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg tatctaaata
     1441 attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta acgggaatcc
     1501 tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa tgaattttga
     1561 ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa atggtttatc
     1621 atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag aatcaaagaa
     1681 ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac cagcgttccc
     1741 atggaatacc gcactaatgt aaccttgtgt ttgacttaaa atatcaggtg cagcttggaa
     1801 agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa acaatgaatt
     1861 ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga aaaagttgtc
     1921 aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa cttcatgctg
     1981 gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc tttctaaatg
     2041 gatataaatc acattttttc ctctagcgag cccgaacaaa tcgtcatttg gtgctgcata
     2101 atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt ctgcccgcac
     2161 ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca agccaagata
     2221 tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat cagcttccgc
     2281 cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa aagccattct
     2341 tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa cagtcaagat
     2401 aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg cactttctcc
     2461 cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga agtccgtgaa
     2521 ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat aaatgaccat
     2581 catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa gtaataacaa
     2641 tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt cgattccgag
     2701 atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacga ctagtaaaga
     2761 aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca agataatctt
     2821 cctttctgca cttcttaatc acagaagtgt cctgaaacca tgttacacag ctcacttatt
     2881 ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt ttatgctgtt
     2941 tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt cctatgtttt
     3001 ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa aagtaaaaaa
     3061 aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag accttgctca
     3121 tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc aatttatcgc
     3181 aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc agcagaacat
     3241 cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa aaagaacttt
     3301 ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag gagacgggat
     3361 cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt ataacgaaac
     3421 gttatatgca catcgtgaat tattgattca agcttttcgt gaaatctacc cggaaatcat
     3481 aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc agttcattta
     3541 cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat ttgccgttta
     3601 tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac ggggatcatt
     3661 agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata ccggcgcctt
     3721 ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc tagcaaaacg
     3781 cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg atcagcaaaa
     3841 aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac aatttttata
     3901 tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag tctttcgcgt
     3961 agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag aagacgggat
     4021 acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa caatgattga
     4081 agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc gtctgccaaa
     4141 agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt ttgtttatca
     4201 aattaaaaat aaccattaga aggagcgatg cagaatggtt cgggtaaagg gattgaatat
     4261 aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag aggaagtatt
     4321 acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc gagtcgatca
     4381 ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta tccatgagat
     4441 gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg gagaacaaga
     4501 catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag gattagtaga
     4561 tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga tagaggaaat
     4621 caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa caccagcaga
     4681 ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccgata tcggaaaact
     4741 ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga ctagacgggc
     4801 aaatgctttt ttctccatgc ctattattac gatgtccatg ggaaatcttg gtaaaatttc
     4861 tcgtctggtt ggcggaacga cgggttccgt tatgacattt ggttccttaa gcagcgattc
     4921 tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc aattattaaa
     4981 tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa atagtaaaaa
     5041 cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac gaatatgagt
     5101 gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa agccaatctc
     5161 gtatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt gctgtgatac
     5221 cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat aaaaaagtag
     5281 aaatgctcca tttaaaagat ttggaagcaa cgaccggata catacgtggt ggctgctcgc
     5341 ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa agctatgaac
     5401 aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct caagatatct
     5461 gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt ataaaagaaa
     5521 gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttttatac attatgctct
     5581 ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga tattaaagcc
     5641 gccagtataa ccattgacat ccagtaattc tcccgcaaaa tacagaccat caatgatttt
     5701 actctccaat gttttaggat aaatttcctt tagagaaacc cctccgccag ttacaaatga
     5761 tttctcaata ggaaaagttt tgttgattga tagcttaaac tctttgcata gttgaacaaa
     5821 atcttgaatt tgtttttgag aggcttgttg tcctgtcaga tgatccattt caagccgctc
     5881 aagaaagaaa actaacaatc gctcaggcaa tataccatgc caagcattga ccaagttctt
     5941 tttggttgct ttactttttt ctgtcaatat atgaataagc tcttgtttgg tttgtgtagg
     6001 gaaacaatcc agagaaagtg tcactggttc tcccgttttc tctaattctt tattgacaaa
     6061 acttgaacaa cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa gtaaatccat
     6121 cgtatgttca gtcaatacac gacctttttt atttaaaacg cgtaaagtga tgtcttgtaa
     6181 agaaagccct tgaagtgttt tctcctgaat atacggttca tccgatatca agggggactc
     6241 tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt acccatctcc
     6301 agtagaacca gtagatggat aagtccgtcc accagttgtt aagataatgg caggagcatg
     6361 gaactcttcg aattccgtgc gtataccgta aattttatgt tctttgtgga tcaatttctc
     6421 tactcgagta gagaatagaa gcgtcacatc tagttctttc agacgattga gcaatgcttc
     6481 gataatcgtt tttgatttat ttgttacagg aaacatccgt ccatgatcct cttcttttaa
     6541 atgtacgcct tgtgattcaa agaagttcat gatatcgaaa ttattccatt gcgcaaacgt
     6601 actatataaa aactttccat ttccaggaat atgtgcaatc aaatcatcaa caggacgatt
     6661 gttcgtaaca ttgcaccgac caccacctgt catcagtagt ttttttcctg cttttttgtt
     6721 tttttcgatc aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg ccatcatccc
     6781 gcttgttcca gcgccgacta cgatcacatc atacagtttt tccattccct cacctcaagg
     6841 aaagtgtagc atatctgaag aaagaaaaca gaaaaaaatg ttttgagcca aaagagaaaa
     6901 tgagctaaaa tgagggaaga taagcaaagg agcgtggaac gatgacaaac gcaacagaat
     6961 atgttcaagc aattcaagaa aagctacatc aaaaggatca agggcaagaa gaatttttac
     7021 aagcaatcga tgaattcatg cccacggtta tgtctttttt ggacactcat cccgaatata
     7081 tcgaaaagaa tatcttagaa cttttaacgg aaccagaaag agttattcaa tttcgagtgc
     7141 cttggcaaga tgatgaagga aactggcgag taaatagagg gtaccgtatc caatacaact
     7201 cagctatcgg cccttataaa ggtggattgc gcttccatcc tagtgtcaat ctaagtatct
     7261 tgaaattttt ggcttttgaa cagattttca agaatagttt aaccggtctg ccaatcggtg
     7321 gaggaaaagg cggaagtgac ttcgatccta aaggaaaatc tgataatgag atcatgcgtt
     7381 tttgtcaaag tttcatgctg gaattgtcaa aacatatcgg cccatcgatt gatgttcctg
     7441 cgggagatat cggtgtagga gctagagaaa tcggctattt gttcggcgaa tataaacgat
     7501 tgaaaaaata tgatactggt gtgttgacag gtaaaccttt ggatttttgg ggaagtaaga
     7561 tacgtacgga agctactggt tatggattag tgtattatgt caaacatcta ttgaacgaag
     7621 aaaaagattc atttaatcaa aaaacagttt ttgtttccgg aagcgggaat gtagccattt
     7681 atgcaatcga aaaagtgcaa gagctaggcg ggaaagttgt tacatgttca gattctagcg
     7741 ggtacatcta tgatccagaa ggaatagatg tgaaactatt gaaagaagtc aaagaaatca
     7801 atagacaacg tttgacggaa tacgcaaacc aacgtccttc tgctatttat catgctggag
     7861 aatctgtttg gacattgaaa gaaaaagcgg atatcgcttt gccatgtgct actcaaaatg
     7921 aaatagacga aagcttggcc gaaatcttag tagaaaatgg gatcaaaatc gtagcagaag
     7981 gcgctaatat gccatgtaca gtaaaagctg tttctgttct tcatgaagca ggcgtgtggt
     8041 attgtccagg aaaagctgct aatgctggag gagtagcagt atctgcttta gaaatgagtc
     8101 agaacgtcca gcgattgact tgggaaagag agcaagtgga tgagcagctg gatcaaatca
     8161 tggaaacgat ttaccatacg tgtcgccaaa cagcagccga gtacggcgac gaaaaaaatc
     8221 tgcttcttgg agcaaatgtt gctggatttg aaaaagttgc caaagcaatg gcagcagaag
     8281 gtttagtttg aaaaatgaaa ctttcttgga aatattgtca gcattttgtt tatttgcttg
     8341 aaaaataata aaaaaaaacg taaagtatta cctgtagagt aaacctataa ggaggagaac
     8401 aaatggcaca cattcatttt gactattcaa aagtagcacc gtttgtcaac gaacaagagt
     8461 tagggtacat gcaaagccaa gtaacagcag cccacaacga attacgggaa ggtactggcg
     8521 caggaagcga cttccgcggc tggatcgatt taccaacgaa ctatgacaaa gaagaatttg
     8581 cacgtatcaa agaagcagct aaaaaaatcc aatctgattc tgaagtatta gttgttatcg
     8641 gtatcggtgg ttcttattta ggcgctcgtg cagcgatcga cttcttgaat cacacattct
     8701 tcaatttatt agatagcaac acaagaaaag caccacaaat cttctttgcc ggaaattcaa
     8761 tcagctcaac atatattgct gacttgatcg aagtaatcgg tgatcgcgac ttttcagtaa
     8821 acgttatttc aaaatctgga acaactactg aaccagcgat tgcgttccgt gtattcaaag
     8881 agcttcttgt taagaaatat ggacaagaag aagcaaacaa acgtatctac gctacaacag
     8941 ataaagcaaa aggtgcagtt aaagtagaag cagatgcaga aggttgggaa actttcgtga
     9001 ttcctgatga catcggcgga cgtttctctg tattaactgc tgttggtttg ttaccaatcg
     9061 ctgttagtgg tgcagatatc gatgcattga tgcaaggtgc cgctgatgca agcaaagcat
     9121 actcaagcga caaactagaa gaaaacgaag cttaccaata cgcagcaatg cgcaacattc
     9181 tttaccgtaa aggcaaagta actgagttgt tgatcaacta tgaaccagga atgcaatact
     9241 tctcagaatg gtggaaacaa ttgtatggtg aatctgaagg aaaagaccaa aaaggcatct
     9301 atccttcaag cgcaaacttc tcaactgatt tgcactcttt aggacaattc atccaagaag
     9361 gacgtcgcaa tatttttgaa acagttgtga aggttgaaaa acctcgtaaa tcaatcacaa
     9421 ttcctgaaca agcagaagat ttagacggtc taggctactt gcaaggaaaa gaagtggact
     9481 tcgtaaatac aaaagcattt gaaggaacat tgcttgctca tacagacggt gacgtgccta
     9541 acttgctagt caaaattcca gaaatggatg cttatacatt gggttataca atgtacttct
     9601 ttgaaattgc tgtaggtatt tcaggttact tgaatggaat caacccattc gaccaaccag
     9661 gtgtagaagc ttacaagaaa aacatgttcg cacttctagg caaaccaggc tttgaagatc
     9721 ttgctaaaga attgaacgaa cgcctgtaag ataagggttt ttcgctagat ttaaataatc
     9781 agtgatagaa acatgttcgc tttaaaatga aaactggtac ccatttaggt actcaaacat
     9841 actgcatcgt ttattcagaa taaggggctg tgacaagacg tttgtcacag tcccttgttt
     9901 gtatagaaat cgattgattg ccttgctctt tagtttgttt taggacttta agaacagtga
     9961 ggctgacttc gaaggttaat tctgtttttt tgttttctag aatcagtaag caaaaaagca
    10021 ctcacttcga gtgcttt
//
LOCUS       DACEKH010000093         9940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEKH010000093 DACEKH010000000
VERSION     DACEKH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.097"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..173)
                     /locus_tag="IW662_002225"
     CDS             complement(<1..173)
                     /locus_tag="IW662_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0867035.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IW662_002226"
     CDS             complement(593..1714)
                     /locus_tag="IW662_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAQ0867036.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /locus_tag="IW662_002227"
     CDS             2042..3451
                     /locus_tag="IW662_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ0867037.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IW662_002228"
     CDS             3448..4176
                     /locus_tag="IW662_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0867038.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IW662_002229"
     CDS             complement(4304..5215)
                     /locus_tag="IW662_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0867039.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IW662_002230"
     CDS             complement(5232..6239)
                     /locus_tag="IW662_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0867040.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IW662_002231"
     CDS             complement(6236..8365)
                     /locus_tag="IW662_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0867041.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8495)
                     /locus_tag="IW662_002232"
                     /pseudo
     CDS             complement(8370..8495)
                     /locus_tag="IW662_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8666..9853)
                     /locus_tag="IW662_002233"
     CDS             complement(8666..9853)
                     /locus_tag="IW662_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ0867042.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         3020 a         2041 c         1738 g         3141 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttttcagtca agtccacaat cttgtgtaaa
     8521 agttcaacct catcaaaagc acaatgtttt gcgttgtgaa cctaactgcc atgcctttct
     8581 ttctgaggag agaggtcagg agcagtagcg cagcgactct tgatgtctct cctcagaaag
     8641 aagagactcg cacaggttca tggtgctaaa tgttattcga attaaattct aaatattttc
     8701 gtggagaagc cagccactca gtgtcttttt ccatcaaaag tgttccaatt atccgagtga
     8761 tggaattgat atttggaaaa atccgaatca ctcgttctct ccggcgaatc tcttcgttca
     8821 accgttccaa cacattggtt gtacgaatcc ttcggcggat attttcagga aaagacaaaa
     8881 tttgaatagc atcttcaaag ccgttttcta aaatgtcgca agcagcactt aattttgaat
     8941 cacaaccata tttttccaag aagtgctctt ttctttcacg tgtcaattca agttcagagg
     9001 ctttaaagat actcttcaac tcgtctttta catcagaaga aactttcttt ggcagcttat
     9061 caaaaatatt tctcaaaaag tgggcttggc atctttgcca gctcgcacct aaaaagttct
     9121 tgcgaacagc cttaactaac cctttgtgtg catcagaaat gaccagtttt acaccgaaca
     9181 aacctcgttg aacgaacgat tgaaaaacgg tatcccaatt atcttccgat tcgccgtctt
     9241 gaacatcaaa accaagaatc gtacgtcgtc catcagaatc aatgccgata ccaatatata
     9301 caccttttga gacaatacga tgattttccc ttactttcat atacagggca tcacacatga
     9361 aaaatggata ttccgagcct tctaggctcc ggtttctcca ctcaaaaaca aatggatcca
     9421 actgtttcat cagatttgaa acgaatgatt tagaaacagt cgcaccatcc gttagcagtt
     9481 caactgtttt agttactttg cgagtggaga caccagaaat gaccatttca atcatggcag
     9541 cgatcaaagc tttttcgctt cgctgatatc tttcaaagat agtaggacta aacttaccgt
     9601 ctctggttcg tggaacatga agttctaacg taccaattcg agttgtaaac gaacgatcgt
     9661 agtaaccatt tctttgtgat tgccgttctt ctgaacgttc ataggcattt gcctgaatat
     9721 attgatttcg ttgttcttcc atcagctgat taaacatgat agtcataagt gtttttgcgg
     9781 tatcattcgc accgctttcg tttataattg cttgaatttc ttcgctttca aaagtaaaat
     9841 gtacttgagt cattgatatc agtcctattc gagtatgttt tctgcaaaaa acattgtact
     9901 cctaactgag atcaatggct tttttgatgc ttcactttta
//
LOCUS       DACEKH010000094         9857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEKH010000094 DACEKH010000000
VERSION     DACEKH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.098"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            40..3267
                     /gene="fss3"
                     /locus_tag="IW662_002234"
     CDS             40..3267
                     /gene="fss3"
                     /locus_tag="IW662_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0867043.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3380..3694
                     /locus_tag="IW662_002235"
     CDS             3380..3694
                     /locus_tag="IW662_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0867044.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3707..4081
                     /locus_tag="IW662_002236"
     CDS             3707..4081
                     /locus_tag="IW662_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0867045.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4091..4426
                     /locus_tag="IW662_002237"
     CDS             4091..4426
                     /locus_tag="IW662_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0867046.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4509..5849
                     /locus_tag="IW662_002238"
     CDS             4509..5849
                     /locus_tag="IW662_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0867047.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            5927..6601
                     /locus_tag="IW662_002239"
     CDS             5927..6601
                     /locus_tag="IW662_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0867048.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6834..7268
                     /gene="dcm"
                     /locus_tag="IW662_002240"
     CDS             6834..7268
                     /gene="dcm"
                     /locus_tag="IW662_002240"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0867049.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            7269..7976
                     /locus_tag="IW662_002241"
     CDS             7269..7976
                     /locus_tag="IW662_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0867050.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGMLLGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            7966..8256
                     /locus_tag="IW662_002242"
     CDS             7966..8256
                     /locus_tag="IW662_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867051.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8515..9699
                     /locus_tag="IW662_002243"
     CDS             8515..9699
                     /locus_tag="IW662_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0867052.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9714..9833
                     /locus_tag="IW662_002244"
     CDS             9714..9833
                     /locus_tag="IW662_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0867053.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         3256 a         1661 c         2239 g         2701 t
ORIGIN      
        1 tcttattttt ttgtgcttaa tttgaaagga gaacatttca tgtataaaaa acaaaaaaag
       61 aaattatgtg tattaactat tttgagtttg gctgttcaag caattgttgg agcaattgct
      121 cctacgatta cttttgctga tgaaattacc catccacaaa cggtaaccgt agaactagat
      181 ttagcacatc aatatgctgt tgaaggaact tttagtgatg gtcgtccaat gtcagaagtc
      241 actgttccgc attatgcagt gtacaacggc tttaaacaag atattttctg tattgaacca
      301 ggagtcccga tttacaatga gttcactcct ggctatgaga aaaacccgtt accagatatg
      361 tccgataaag ccaaattagt ttccgtttta tggaaaaaag taggtacaga tgcagatact
      421 catattgtgg cacagaagat gatctggcaa gaagttaatg ggtataccct tcactcaata
      481 aaacgatcag atggtagtgc cgtaaatatc gcagccattg aggcaaaaat aaatcaagcc
      541 attgacgatt atcagaagaa accaaccttc cataatagta cggctaaaac agtattaggg
      601 caatcgacta ctgtgacgga tacgaataat ctgaatttgt cagagttcga tgaggttgtg
      661 gaaaatgccg caaacattga ttatcgtgtg aatggtaatc agttgataat caccccaaat
      721 gccaattcaa aagaaagtgg tgtgctgacg cttaagaaat ctgctggtac cggaacacct
      781 gtcgcctata aaaaggctgg ccaacagact ttaatggccg gggcgattga taaacctaat
      841 acctacacaa tcaaaattga tgtggaaacg gagggttctt taaagatcaa aaaagtcgat
      901 aaagaatcgg gtgatgttgt accaggaacg gttttccatt tagactttgg aaaaacttta
      961 cctgcaaaag acgtgactac tgacaaagaa ggcattgcta cattggatga gattcctcat
     1021 ggtacaaaag taaccattac cgaaaaatca gtaccggcgc cttatacgat tgataccacc
     1081 cccatgacta ccaccattaa agcaggcgaa accatttacg tgacttcaaa aaacgcacgg
     1141 gaaaaagggc aaatcattct ggataaaagc ggagttgaaa cagggagtga tctttggaat
     1201 gacaattatt ccttagctgg taatacgttt gccattcgca aagacagtcc aaccggtgaa
     1261 atcgttcaag aaatgaccac ggatgaaaac ggtcatgcgg aaacaccaaa agagattgct
     1321 aatgcgttgg aattgggaac ctattatgtg accgaaacta aagctagtca tgggttcgtg
     1381 aatacgttca aacctgtaaa agtcgaattg aagtatgcca atcaaaccgt agcccttgtt
     1441 accagtaacg taaaagggca aaatcaagaa gtcactggcg aaaccacttt gacgaaagaa
     1501 gacaaagata ctggggataa agcacaaggt aaggcagtct ttgagggtgc tgaatacacc
     1561 ttgtttactg ctaaggatgg aaaagctgtt aaatggagtg aggcttttaa accagagtta
     1621 gcgaagggaa cgaaaggttc tgatgaaacg gtcactttga ctttagacga aaagaaccaa
     1681 gttgccgtta aacatttagc tatcaacgag tattattggc aagaaacaaa agcgcccgaa
     1741 ggatatagcc ttgatgaaac aaagtatcct gtatccatca aaaaggtgga tgatagcgaa
     1801 aaaaatgccg tgattacccg aaatgttacg gcaaaagaac aagtgattcg ctttggcttt
     1861 gatttcttta aatttgctgg atcagctgcg ggcactaccg aaactggctt caatgatctg
     1921 acctttaaag tgtcgccatt ggaagggacc aatgaaatca caggtgccga agatgaagcg
     1981 accacagctt ataacgagca attagggttt gatggttatg gcaagtttga aaatcttcct
     2041 tatggggatt atttacttga agaagtagaa gccccagaag gttttcaaaa gattacgcca
     2101 ttagaaatcc gttctacttt taaggaaaac aaagaagact ttgtgaagag tgagtatgtc
     2161 tttaccatta ccgaacaaga ccaaaaacaa ccaattaaaa cggtgaccgt tccttacgag
     2221 aagctgacga acaaagcatt ttctgttagt ttgaaccgtt taatgctcta tgatttgcct
     2281 gaggaagaag atagtttaac ttctcttgcg acttggaaag acggaaacaa agaattaact
     2341 tctctggatt ctaccgagct agttgataaa ttgagttaca atctacatga aatcaaagaa
     2401 gactggtatg tggtagcgca agccattgat gtggatgcga caaaagctgc ccaagaaaaa
     2461 gatgaaaaag ccaaaccagt ggtgattgca gaaacaagcg caacgttggc gaacaaagag
     2521 aaaacaggaa cttggaaaat tcagcataaa ttaaccgccg aacaagtttt aaataaaact
     2581 attgtcttat tcaactatgt gtatgaaaat aaggaagctt ttgaagcagg cgataagcca
     2641 gtagcgaagg atgttagctt gaataaccaa gcccaaactg tcagttgtac agtggaacac
     2701 catgtttcta tccaaacaaa ggcccaccta gaaaacggtt ctcagacctt tactcatggt
     2761 gatgtggtgg atatgtttga tgatgtgtcg attactcatg atgtactgga tggctcaaaa
     2821 gaagctttcg aaacaattct gtatgcttta ttaccggatg gtacaaacaa agaaatttgg
     2881 aaatctggca agattgatta tgaagtaaat gataaagaat tcaccaaaac tgtgcttgcg
     2941 aaaaaagtag ataccggaaa atatccagaa ggaaccaagt ttacttttgc ggaaatcaat
     3001 tacgataaag atgggaatat caatggaaaa cacaatgaag atttgaaaga aaaatctcaa
     3061 accttaacac caaaagaagt tccaaccata ctaagtacgc cgaaacaacc ggaaacacca
     3121 actgttccaa gtggctctca agaatctagt cccacagtga agaccttccc tcaaaccggg
     3181 gagaaaaatt ccaatgtttt actgttcatt ggttttactt tgatttttgc gacggcgggc
     3241 tattatttct ggaatcgccg gaactaaggt gatgagatga tacaagtaaa tcctaagaac
     3301 caacgtgcgc gaccgcctcc taaaaaagta gtcgcgctat ttttgtatcc aaaactaaca
     3361 atgaaaagag gaaaagaaga tggaattgaa atttgtcgtg ccagatatgg ctgaaacctt
     3421 tggaaagtta agttatgccg gtgaaggaga aattataaca gaaggatatg gacggaatac
     3481 tacggtgatt ggtcgcagtt atcatttgta ttccagtaag caacgggccg atgatattga
     3541 ggtggtagta gctgcggaag ccggtgaaaa ggattttgat caagaccaac cgctaaaagc
     3601 cgtgaatccc catttggttg ccaaaggcta tgagattgag aatcgtgggt ttactgatta
     3661 cgtgttgtat gttgatgatt tagtgaaagc ataggaggaa aagaagatga gattaacaga
     3721 aggtattgtc gtagattcag gtttaacatt tgggaagttg cgtttttccg cattacgccg
     3781 agaagtacgg aagcaaaatg aggatggaac aattggtaat gaggtaaaag aacgaaccta
     3841 taatttaaaa tcctctgcac aagggcgaat gattcaagtt agtatcccag ctaatgtacc
     3901 cttacgcgag tttgcttatg atgcagaggt agagttggtc aatccaatcg tggataccgt
     3961 tgccaattat gtttttcgag aaggaactac cgttaattgg ttcattaagg cggatgattt
     4021 agtcttgaag cggcagccga atcaaggaaa tcctacgaac caaaatgaaa gaaagaaata
     4081 ggtgaagatt atggaaatta atgttgaaca tattttagat tgtctcgatc aatatggaaa
     4141 aggcgagctg actgaggaac aactcgttaa agcattaacc tctgatgaaa aaatgtttct
     4201 gattatacat caaggtttac ttggagaaaa tagcgacagt gaagaagact ctgatgtact
     4261 agactggtta ggagaagaag agtctttctt tatagttgtc gaagtaaatg aaaacttatg
     4321 tcggcaggca gaatccattt tggaagaaat tggtgtggag atgccggatg ctattgaagg
     4381 ctttttaaat caattggttg aaacgaaaca actcccctcc tcgtagcagt aaatgactag
     4441 cataactttt tgaaattgcc taatgaaatc ctattgggct ttttcgattg gaggtggaaa
     4501 ccatcaaaat gtacaaagga catcgcatac gcgcaggaga tcagcacttg gtttatcact
     4561 ttgttcttgg ctggctactc gctctgttca tcggttggat gagcgtcttt tattttcaag
     4621 agtttagaca atttgatatt tctaagctat cactttctac tattgaaatc gtttggtcta
     4681 taaaagattt agtatgtctg cttggaagtc tcgctttttc aggagctatg atattgcttt
     4741 atattcactt ttttcttgat cattggcgaa gcttgtggca tcgacaaaag ctagctcgga
     4801 tgattctaga gaaccattgg tatgaagtga aacaaactca aagtgaaggc tttttcaaag
     4861 atttgaatag tagccgaacc agagagacta tcagttactt ccccaaaatc tattatcgaa
     4921 tgaaagatgg attactgtct attcgcgttc aaatttcact tgggaaatac caagatcagc
     4981 ttttaaaatt ggaaaagaag ttagaaagcg gactgtattg tgagttagta gaaaaggaac
     5041 tcaaagactc ctatgtggag tatactttac tgtatgatat gatcgccaat cgaattggga
     5101 tagacgaagt agtggcagaa aatgggactc tgcgattgat gaaaaatcaa gtatgggctt
     5161 atgattcttt accccatatg ttaatcgctg gtggcacggg tggagggaag acctatttcc
     5221 tcctcaccat tatcgaagca ctattgaagt cagatgcaga attgtttatc ctcgatccca
     5281 aaaatgcaga tttagctgat ttaggtacgg tgatgcctca tgtttattca caaaaggaag
     5341 aaatttctgc ttgtgtggaa gatttctatg aacgtatgat ggctcgtagc aaggcaatga
     5401 aagaaatgtc taactacaaa acaggagaga attatgccta tcttggactt ccacccaact
     5461 ttttaatctt tgatgaatac gtggcttata tggagatgtt aacgacgaaa gaaagtgcgg
     5521 tgattttgaa taagctcaaa caaatcgtga tgttgggtcg tcaatctggt ttctttctga
     5581 ttttggcttg ccaaaggccg gatgcgaaat atttaggaga cggaatccgc gatcaattca
     5641 actttcgcgt ggctttgggt cgtatgagtg aactcggata ttccatgatg tttggggagg
     5701 tcgaaaaaaa cttctttatg aagcacatca aaggtcgagg ttatgtggat acaggtggca
     5761 gtgtgattag tgagttttat acgccacttg taccaaaagg gtatgatttc ttagattcaa
     5821 ttagtaaatt agaaaagatg aatgattgag tgatcttcaa tactgacttt atacttcaag
     5881 taacgtataa tacttttata tagtaggaag aaaggggata attaatatgg aaagtaatga
     5941 ttcttattta gagtatgcaa aagcaaataa ggataagttt atttcttata ttatcaaagg
     6001 gaaatccccc gaagttggac gagacgcagt ttttatggca gggagtcctg gggctggaaa
     6061 atcagaggtt gccattggat tagctgagaa ttatgataac catgtgatta ttgatgcgga
     6121 ttactttcgc acccagtttc ctgattataa tggaaaaaac tctagtctct ttcaaaaagc
     6181 atcttcttgg ttggtagagc aggcatttag atatgtctta gatcatggct attcttttat
     6241 tctggatgca acttttgcaa tattaagtgc tgaaaaaaat atctcgcggg cgttgaaaaa
     6301 acattttcga gtgacgattt tctatgttta tcaagaccct aaaatcgctt ggaaatttac
     6361 acaagaacgt gaaattgcag agggacgtca tgtgccgaaa aaggcattta ttaacgcttt
     6421 tttcaaatca agagagaaca ttgcaaaagt aaagagtaaa catccagatg tattgcttca
     6481 tatcataata aaagattata cgaataatat ttccgaagtt cattatgcta cggataatct
     6541 agaattattt ttaccactag ggtatagtag gaaagatttg gaggttcaat tacatgactg
     6601 agaaagaaca agtaacgaaa attgtaaaga aatacaataa gagcattgcg gatttatcgg
     6661 aaaatgctac tgcaaaagag ttcaaaacgg tgattaaata tgtagccgat caggccaatg
     6721 aaaaacaacg gaaattagtt ggattaaata aaaaatagta ggacaaatta ataatgtatg
     6781 agccaagagc aaaatgttct tggctttttt ctttgcaatg aatgaggtaa tgtatgagat
     6841 ttttagattt attcgccggt attggtggct ttcgtttagg tatggaacag gcgagccatc
     6901 attgtatcgg tttttgtgaa attgataaat ttgctagacg tagttataaa gcaattcatg
     6961 atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt atcagatgag tttattcaat
     7021 ccctcggacc agtggacatc ctttgcggcg gatttccgtg ccaagctttt tcaattgcgg
     7081 gaaagcggca aagattttcc gatactcgag gtactttatt ctttgaaatc gctcggttca
     7141 ccactcttct ccgacctaag cttttattcc ttgagaacgt ccgaggattg ctcaatcacg
     7201 aaggaggggc tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgacgtgg
     7261 agtggtaggt gcttaactca aaggcctaca ttccccaaag ccgggagcgg atattcctta
     7321 tcggacattc tcgagacgca tgtaccgaac aagtatttcc tatcattgga ccgtcttcaa
     7381 catctgatca aaacatcaga aatttggtaa atatcaatcc ttccaatcga ggaatgggtg
     7441 gacaagtcta tggttcagat ggcgtagcgc caaccttaac aggtgatgaa ggaatcaaaa
     7501 tcgcactgcc agcaagggat ggaatctctg tagcgggtat gttactggga aagtttgaac
     7561 aaggtaatcg agtttatgaa gttactggaa ccgcccccac cttgtcaacg aaacaaggtg
     7621 gtacaaaaat catgattcga aaaaatgaaa cagaatatca agaggtgaag ccagaagaca
     7681 gtgtgaatct ggcttttcct aattccacaa gtcgccgagg tcgtctggga aaacagagtg
     7741 ttcatacctt actaacagga gaccaacaag cgatcgtcac ggatcagtat caaattcgga
     7801 agctaacacc tagagagtgt tggcgacttc agggctttcc cgattgggct tttgatcgag
     7861 attctcaagt caattcagat agtcagttgt ataagcaagc cggaaactct gtaacagttc
     7921 cggtcatttt tgatattgcc agaagattaa aagagttgga tgtacatggt ttatgatgag
     7981 ttgattggag aaatttattg ggtgattgga aagattcagt cagacccaga attagaagaa
     8041 caattgcgac ggttaaaatt tgatattcgc aagaatggtg tcaaagtcgc tggcgatcca
     8101 tatttaatga atgaggagac ggatgctcga attgaaataa atcaagtaat tgcagaattt
     8161 gagcggattg cggaccaagc aaaagaacca gatattcgtc aatacttgtt agaaatgaaa
     8221 gcagagttgg aagtaaatgg tattacagat gagtaaccta aaccaacaca actgactgtt
     8281 aggcgctgac cgcggaggat tcgcgaagca gaaatcctcc gtggtgctgt gcctgttcat
     8341 ggacggcgtt agccgaccat gatgtctcac ccccgtatct aacagggggg tacaaaaaca
     8401 caaaaaataa gtaagcaacc attaagaatg cttgttgtat caaagatagt aagtaatttg
     8461 tataggtgtt aactcatttg aaaaagttaa caccttgaat ttggaggtga gaacttggca
     8521 caacggaatt tagattatcg gctattaaaa gaccgacgca acgaatacgg cgtttcacaa
     8581 aataaattgg ctacggcttg tggacttagt cggccgtact taaatcagat agaaaatggt
     8641 ggcgtaaccg catctataaa aaccatgagg aaaatttttg accaactgga aagctttaat
     8701 cccgatttgc ctttgacgtt actgtttgac tatgtaagga ttcgctttcc cacaacggat
     8761 gcacgaaaaa ttattcaaga aattctccat ttgaaatttg agtatatgct ccatgaagat
     8821 tatgcctttt actcctatca agaacagtat gtcatgggtg atattgtggt gatgttgtct
     8881 catgaagaag ataaaggtgt gcttttagaa ttaaaggggc gaggttgccg acaatttgaa
     8941 acttttttac tcgctcaaaa gcgtagctgg tacgactttt tcgaggattg tctaaaggcc
     9001 ggtggcgtga tgaaacgctt ggacttggca atcaatgatc gagtggggct attggatata
     9061 cctgagctaa cgaaaaaatg tcagaaggaa gaatgtatct ccttgtttcg tacctttaaa
     9121 agttatcgtt ccggggaact tttaaaggct gatgaaaagg acggcatggg caatacctta
     9181 tatatcggca gtctcaagag tgaggtttat ttctgtttgt atgaaaaaga ctatgaacaa
     9241 tatatcaagt tagggattcc attagaccaa actgagacga aaaatcgttt tgagattcga
     9301 ctgaaaaatg accgtgcttt tcatgcgatt caagatttgt taaaaggtcg tagcattgag
     9361 agcaccacgt tttccattat caatcgttac ttgcgatttg cggataaagt agaaggtaaa
     9421 aggcgaacca actggccctt gaatgaacaa tgggggcgtt ttattggacg gaatcggaaa
     9481 gagattcaat taacttccga gccaaaaccg tataccattg aacgaacctt gaattggcta
     9541 ggccgacaag tcgcccctac gtggaaaatg gctaaggagt tggatcgatt gaatcggaca
     9601 acctatatcc aggatatggt acagaatgca cgcttatcag accgacataa aaagattttg
     9661 gagcaacaaa gtatggcaat tgaaaatttg attatatgaa aagaggaatc gtcatgggaa
     9721 ttgtaaaaga tatttttttg gtatttggtg gcagtatgct tggtgttact gtgatgtgct
     9781 tgatgaatgc aagtgcagca gcggatcgag caatggaaaa gaaaggaaag tgacaagatg
     9841 aatttcggac aaaattt
//
LOCUS       DACEKH010000095         9538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEKH010000095 DACEKH010000000
VERSION     DACEKH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.099"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            195..617
                     /locus_tag="IW662_002245"
     CDS             195..617
                     /locus_tag="IW662_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867054.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            627..830
                     /locus_tag="IW662_002246"
     CDS             627..830
                     /locus_tag="IW662_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867055.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1041..1625
                     /locus_tag="IW662_002247"
     CDS             1041..1625
                     /locus_tag="IW662_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0867056.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            2567..4429
                     /gene="ltrA"
                     /locus_tag="IW662_002248"
     CDS             2567..4429
                     /gene="ltrA"
                     /locus_tag="IW662_002248"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0867057.1"
                     /translation="MENVSASHSIRHMEYYGMIDSFDNLYKESRDDHIFNNLMEIVTS
                     PNNILLAYRNIKGNSGSVSPGVDNKNIDDLKEIPNADFIRIVQGKFSEYKPQPVKRVE
                     IPKPNGKNRPLGIPTIWDRIVQQCLLQVLEPIMEAKLHDKNYGFRPNRSAHHALAQAV
                     RLAQLSKLTFVVDIDIEGFFDNVNHSKLIKQLWTLGIQDRWLLGVIRAMLKAPIIHKD
                     GRIEHPKKGTPQGGILSPLLANVVLNELDWWISSQWETHPTRHNYDCYQANQGYWNKG
                     NKYRALRRSKLKEIYIVRYADDFKIFCRRRSDADNIFIATKLWLKERLKLDISLEKSK
                     VVNLKKKKSEFLGFTMKLVRKRKTFVIETHMCPKAMKKVSDNLTKQIKVIQHTPTKED
                     LQKQLVKYNSMVIGIHQYYRRATHISVDCPKINQRIRIIIKNRLDVKKNGSPPSDFVK
                     KNYARSKQLRWINGYPMIPLGFVKHSHTSLPKMGICKYTPEGRVLIHKYLTFPIDIIN
                     QLMNMNNPDESIEFRDNRVSKYSAQRGRCRVTKELLELEDIYCHRIIPKNLGGKDNFQ
                     NLVIIHQNIHQLIHENDRTRASLLITRMRITGAQLEIINQFRKKAQKCELSLNL"
     gene            4511..5806
                     /locus_tag="IW662_002249"
     CDS             4511..5806
                     /locus_tag="IW662_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0867058.1"
                     /translation="MEIISKDYLSLSSVECIERNLDPLTTELVVMSRSDNTGSGLILA
                     SSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNIFRRYVADEDL
                     LIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIATVGVGDNPLL
                     AKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGILSIRD
                     LANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYTRRN
                     EIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKIPITDNTKELQ
                     NYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDKIRQ
                     KYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            5799..6149
                     /locus_tag="IW662_002250"
     CDS             5799..6149
                     /locus_tag="IW662_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867059.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            6146..6325
                     /locus_tag="IW662_002251"
     CDS             6146..6325
                     /locus_tag="IW662_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867060.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            6461..6754
                     /locus_tag="IW662_002252"
     CDS             6461..6754
                     /locus_tag="IW662_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0867061.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFNSNQQRKVKKQID"
     gene            6980..7798
                     /locus_tag="IW662_002253"
     CDS             6980..7798
                     /locus_tag="IW662_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0867062.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            7817..8224
                     /locus_tag="IW662_002254"
     CDS             7817..8224
                     /locus_tag="IW662_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867063.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            8544..>9538
                     /locus_tag="IW662_002255"
     CDS             8544..>9538
                     /locus_tag="IW662_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0867064.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWF"
BASE COUNT         3527 a         1473 c         1782 g         2756 t
ORIGIN      
        1 tgatggagga aatagtatgt gtcacttttc tttttaaatg gcaatatttt ttgtaaataa
       61 atgtcaatca agaaaattta aacgattatg acattaaaaa tatattttat gaattttcta
      121 gtatataact tgatttatct gttaagattt attaagaaaa cgttttctta tttttgattt
      181 cgaggtgata gacaatgaaa aataagttta ttttatcttt aggtatttta actattggaa
      241 ttagtgtaac tggaactaca attcctacat ttgctgatag tacaaatgaa ccaactactg
      301 tagtagataa taaccaagaa ttaactcctt tagataggaa cggaatagcc attggtaacc
      361 caactaacga cttaactgtt actgaaaatt atttaatagt ttctccatta cttgctcgag
      421 cagcttctaa aacgatacga gtgacaggaa cgatgatttt tgataaagtt ggttggcctt
      481 ctagacctcc tcaatcacgg aattggaaag aaaaaagaaa tggttattgg tactatggaa
      541 aattgtattt acaaagttat aaatcagctg gaaacaattg gattgcgact tattcaggaa
      601 ccttaactag aaagtaggga ttttttatgc cagaatttgt gaacacacca aatttttatc
      661 aagatagcga tattctaagt aaaattaatt taccgaaaga agaatcagct ttacaagttc
      721 agcgaacttt acaggagaca caaaaaattc aaaaatcaat ttctaatcta gaagattctg
      781 aagaaaaaag aaaaaaaaga gaccaaaaaa catatcaaaa agaaaaataa tgattttaaa
      841 aaaagagtgt ctagtcgaca ttcttttttt ctaattgaaa gtgttcataa aatcacccat
      901 ttctggacac tttctttttt tgctttattt ttcaaaaaaa caattcattt catgttcata
      961 aacccataac aatatctatg ttcagttaaa ctatatggat aaagttatgt tacaataaaa
     1021 ttaatgaagg agtgattttt ttgaaaatag gatacgcccg ggtatctact ggtttacaaa
     1081 atttggattt acaagaagac agcctaaata aatttggttg tgaaaaaatt tttactgatc
     1141 atatgagtgg ggcaaaaagt aatcgaccag gattagaaat ggccattgat tttgttcgct
     1201 ctggcgatac acttgtcgtt tggcgcttag atcgtttagg tagaaatatg gaagatttga
     1261 tttcaatcgt caatcgactg aatgagcgtg gtgtgagttt tcatagtatt caagaaaata
     1321 ttacgatgga caaatcaagt tctacaggac agctcatgtt tcatttgttt gcggcatttg
     1381 cagaatttga gcgtaatttg atcctggaac gatccgcagc aggacgagca gctgctcgtg
     1441 caagaggacg gtttggtggt cgtcctgaaa aattgtctga acaagatttg gaattactga
     1501 agactttagt ggatagcggt acacctatta agactattgc ggaacggtgg aacgtctcaa
     1561 gaaccaccat ttatcgttat ttggcaaaaa tggaggagaa aaatggaaag acaaattctc
     1621 attagttttt gtcatttaat gctgaaaatt tttgccattt atctagaaaa gtgacaatag
     1681 tatgagaatt ttttgtaagt gtctactgat aacttattct caaatattta gctatttctt
     1741 ctttacaaca tcgaacatat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
     1801 attattcaaa agagccagta aatgattact ttttaatcga tatgaaaagc ttttacggtg
     1861 cgaaacgttc ctaactgaaa aggttaggca cacaagactt tcttgtgtag aactaatgat
     1921 ttgaggagtg aaatgacggt gtaacgacct gaagcgctcc ctctgatact ccgattggca
     1981 atacctttag ttagaatagg aattgtcaaa agctcggtaa agtcggctga gagttaccgt
     2041 agttgcatta tgcaatcgaa agctgttcag cggtgaatgg tgtaacctat aggtcgggag
     2101 tccagaaatt actcatggtg agaatgtgca gttgcactga cgaactcgcg aatgtacgaa
     2161 tctataagct tggggtgcag aaatgtatct tacccgcaca aatattactg tgacaaaggg
     2221 tgtagcttgc ggttaagtaa gaatcgtaga tatgaacaac cgtgcaatgt ggtagacatt
     2281 ggaaagttac aggttcagag tgagcaccta aaagtaaatg cagggataaa atcattggaa
     2341 cgttgaaagc tagtgtcgtg gaggactaac ttcctttgaa acggttgaga cgaagaataa
     2401 taagctcatt tacactagtg aaagtagtgg cacagtacct atgaagcagt gataataaac
     2461 tgtagaggga tagccacaag tcaatttact ctactaacag taatgatata atccatagaa
     2521 tcgagtatga caaaataaac gccgacgtcc gaaaggaggc ggtgctatgg aaaacgtatc
     2581 agcttcacat tcaatccgtc atatggaata ctatggcatg attgacagct ttgataatct
     2641 atacaaagaa agtcgcgatg atcatatttt caataacctt atggaaattg taacctcgcc
     2701 aaataatatc ttactagcgt atagaaatat taaaggaaat tctgggagtg tatccccagg
     2761 cgttgataac aaaaatattg atgatttaaa agaaatacct aatgcggatt ttatcagaat
     2821 cgtgcaaggc aaattttcag aatataaacc acaaccagtt aaaagagttg aaatcccaaa
     2881 gccaaacggg aaaaatcgcc cactcggcat cccaactatt tgggatagaa tcgtacaaca
     2941 gtgtctacta caagtcctag aacccattat ggaagcaaaa ttacatgata aaaattatgg
     3001 ttttcgtcct aaccggtcag cacaccatgc acttgctcaa gcagtacgct tagcacaatt
     3061 gtctaaactt acttttgtag tagatataga tattgaggga ttctttgata atgtaaacca
     3121 ttcaaaactc attaaacagc tatggacatt aggaattcag gacagatggc ttttaggtgt
     3181 tattagggca atgcttaaag caccgatcat ccataaggat gggcgaatcg aacaccctaa
     3241 gaaaggaact ccgcagggcg gaattctttc ccctcttctc gcaaatgttg tgttaaatga
     3301 attagactgg tggatatcat cacagtggga aacccacccc acaagacata attacgattg
     3361 ctatcaagct aatcaggggt attggaataa gggtaacaaa tatcgagcgt tacgtagatc
     3421 aaagttaaaa gagatctata ttgtgagata cgccgatgac ttcaaaattt tctgtagaag
     3481 acgtagcgat gcggacaata tatttatagc taccaaatta tggctaaagg agcgcctgaa
     3541 gctcgatatt agcctagaaa aatcaaaagt ggttaatctg aagaaaaaga aatccgaatt
     3601 ccttggtttc actatgaaat tggttcgtaa aagaaagacc tttgtcatag aaactcacat
     3661 gtgccctaaa gctatgaaaa aagtatctga taatctaaca aaacaaataa aggtaattca
     3721 gcacacccct accaaagagg atttacaaaa acaactagtg aaatacaact cgatggtgat
     3781 agggattcat caatactata gaagggctac ccacattagt gtggattgcc caaaaattaa
     3841 ccaacgtata aggattatca tcaaaaatcg attggacgtt aagaaaaacg gatcaccgcc
     3901 tagtgatttc gttaaaaaaa actatgcacg ttcaaaacag ttaagatgga ttaatggcta
     3961 tcctatgatt cctctaggat tcgttaaaca cagccataca tcgttaccta aaatggggat
     4021 ttgtaagtac acgcccgaag gacgtgtgct aatacacaaa tatcttacct tcccaatcga
     4081 tattatcaac caacttatga atatgaataa cccggatgaa agcattgaat ttagggataa
     4141 tcgtgtctct aagtactctg cacaacgagg caggtgccga gtaacaaaag agctcttgga
     4201 gctagaagat atttattgtc accgtatcat acccaaaaat cttggtggaa aagataactt
     4261 ccaaaatcta gttatcattc atcaaaacat tcatcaactt attcatgaga atgatagaac
     4321 gagagcttca ctcctcataa cgaggatgag gattacaggc gctcaacttg agataatcaa
     4381 ccaattccgg aaaaaagctc agaagtgtga attgtcgctg aatctataac tgtatgataa
     4441 ttgttagtaa acattgatgg aacgccgtat gcggtgaaag tcgcacgtac ggtgtggagc
     4501 gggggaaaat ctggagatta tatcaaagga ttacctatcg ctatcaagtg tcgaatgtat
     4561 agaaagaaat ttagatccat taacaacaga acttgttgtt atgagtcgat ctgataatac
     4621 tggttcagga ttgatattag cttcttctcc tgaagcaaaa aagcggtatg gtattacaaa
     4681 tgtgagtaga ccacgtgact taccacaacc atttcctaaa acactacacg ttgttccacc
     4741 acgtatgaac ctatatatca agcgaaatat gcaggtaaat aatattttca gaagatatgt
     4801 ggctgatgaa gatctactga tttactcgat cgatgaatcg attttaaaag tgacaaaatc
     4861 actgaatctt tttacgactg aagaaacacg aagtcagcga aggaagaagc tcgctcaaat
     4921 gatccaagaa cgtattaaag aagagttagg tttgattgct acggtaggcg tcggcgataa
     4981 tccccttttg gctaaattag ccttagacaa tgaagcaaag cataatgaag gatttattgc
     5041 ggaatggacg tacgaaaacg ttcctgagaa agtatggaat attcccgaaa tgacggactt
     5101 ttggggaatt ggttcgcgaa tgaaaaaaag gctcaaccaa atgggaattt tgagcattag
     5161 agaccttgct aactggaatc cttatacgat taagaataga ttaggtgtga ttggattaca
     5221 gctgtacttc catgctaatg ggatcgatcg aacagatata gcaatcccac cagaaccgac
     5281 aaaggaaaaa tcatatggaa attcacaagt gttaccacga gattatacta gaagaaatga
     5341 aatcgagctg gtggtaaaag aaatggcgga acaagtagcg atacgcatta ggcagcataa
     5401 ctgtaaaaca ggttgtgtcc atttacacat tggcacgtcg atcttagaaa cgagaccagg
     5461 gttttcacac cagatgaaga tccctatcac agataataca aaggaattgc agaattattg
     5521 tcttttcttg tttgataagt attatgaagg acaagaagta aggcatgttg gaataaccta
     5581 tagcaagctt gtgtacaccg attcgcttca gctggattta tttagtgatc cacaaaagca
     5641 aattgacgaa gaaaatttag ataaaattat tgacaagatt cggcaaaaat atggttttac
     5701 gtcgattgta catgcttctt caatgttaga aggcgctaga agcattacta gaagcacact
     5761 tgttggtggt catgcgggcg gaaatggggg aattaaaaat gattgattac aatgatcgtg
     5821 gacgtttaaa gtgggcaggg ttttatttat ctgatcatac tgaagaaatt gatgccgaca
     5881 atgaacaacg aaccagtcaa aatttagcaa aagaacaaat gacgactgaa gagattgcgg
     5941 aagtattgaa cgctgcagtc ataaaaaata gatcagtatc tattcaaaaa gaagaacgaa
     6001 acgctgaagg atactatcca gaagatatca ttggcaagat cttaggtagt gatgagctgg
     6061 ggatctacgt tggaaaagac aaaattcact acgacgaaat acgtcatatc gaattttacc
     6121 aggataataa atggagtcga ttagaatgaa cattatccag caatatgagc taaagtatat
     6181 aacctttgat cagctatcag aagagatttg gggttatggc caacgtttga taaatgaggt
     6241 tggtgttgag cgcttctctt tctatgtaga agctgcagca ggttatcata attttaggtt
     6301 ttatatcttc ccgttatata tataaggtat tttacggatt ctatagagtt atctctatga
     6361 atcctttttt gtttttacaa ataggagtag atactagaat aatgcgtgtg ttaaactatt
     6421 attgcaaacg caataataaa agaaagtagg cttacgcacc atgtctatca atacatttgt
     6481 ttactcgcac ccgatcaatg tttacattat caagaattta ggaatcactg tagaacaatt
     6541 ttgtgagtta tatgcctacc cacaaggaac ggttgcaagc tggataaccc gacaacgtag
     6601 aataaaatca ttacctgctt catttgtata tgatctaagt cttgcttcta gtatgaatat
     6661 gaccgatgta tacgaaaagt tattaatact agaaaatgaa tatgatcaat tcaattcaaa
     6721 tcagcagcga aaagtaaaga aacagataga ctagaatagc tattttcgaa aagcaatatt
     6781 taaggtataa gttatacctt taataataaa agtataagtt ataccataaa tatatatggt
     6841 ataacttata cttttattat taaaaaccgc tatatccaaa cgtttttaga tattattatt
     6901 taagttatac ttttaatgtt gaccatttcc gatatataag ttatacttta attatcaatt
     6961 aaagattggg aggatttaca tggatacttt tcaagggact acatttacag ttggcaattt
     7021 taaaggagga gtaggtaaaa ctaagatagt cagcatgctt gcttatgaca atgcaatgat
     7081 aagaaataga aaaacgctta ttattgattt agaccctcaa gcgaacttaa cacaagtcat
     7141 agcaagaact ttcggtataa ccgatatcaa aaccactata acaaatggtg tcgcaaatgg
     7201 agaattagca agtagtatta ttaaggtaaa tgaaaatctt gaccttctgg catgtgacac
     7261 tagttttcga tcattctccg cttttgctaa taaacatgaa aatgaattag accaagttac
     7321 tgtcttgaaa aaattacttc ttccaataaa gaacaattat caagaaatat ttattgatgt
     7381 accaccaacc attagcgaat tttccgataa tgctatggca gcttcagact attcgataat
     7441 ttcatttcaa acagctgaag aatccttaga gggagtcaac aaatatgtaa attatcaaaa
     7501 attcatggtc gatagatacg atcttgattt gcaaattata gatattgtac cttgtatggt
     7561 agaaccaaat gatgattttg atgaagaaat cctagaagaa gcaattgaaa aatacggaac
     7621 cgtggtatcc aaaaatctca ttcattatca aaaaagattg agaaggtatt ctaaaattgg
     7681 tatacattta agaaaataca aaaatggaaa ttttgatcaa tgggattata aagcacacca
     7741 agtatttata aatattttgg cagaactaga tgctagacaa gagtttttaa ataaataata
     7801 aggagttgca taagtaatgc ctaaagattt tgatattcct aaaatgatgg agaatggtga
     7861 atttgagcca ttattaccca ataacaaacg ccaagatgtg aaaataagcg gtgaatataa
     7921 ctttagcaat agtgcattga gtaacacaag aaatattaca ttgaaaaaaa gaggccgacc
     7981 agtttcaatt cctgataaaa gaaacaaggt atcagttcct aaaaagatct ctccggcatt
     8041 aaatagcaaa ctctcaattt tacaagacta tatgacggag ttacaaagtg aaaaagggag
     8101 aatttctttc gaaaaaattg ttaatacatt agcagactca tatatccagc atagactagg
     8161 agtagcaaaa gaagaacaca taagagaaga aatacaagaa gagtttgaaa aattaccaaa
     8221 ataatttttt ttcatgcggt attcaattaa agtataagtt atactagata aatgaatatt
     8281 aaagtataag ttatacatta aatatttaag gtataactta ataaacaacg tgtaaatttg
     8341 cgattaaatc aacattataa aattattatt taaagtataa gttatacatt atttgtattt
     8401 aaagtataac ttatacttat ttttttaata tataacttat accttaaata ctgaaagtgt
     8461 tttattcacc cttaatacat ggtatagttc acttgttgaa aatcctgaat atatacaagt
     8521 ttctaagaag aaggagaaat ttaatgtcta cagaaaatca atctcaaaaa atgataatta
     8581 aaaattgggg atataaagca acgaatgttt atattgaaaa atattataaa tttcctagtt
     8641 ttttaattca gaattctaaa tactctaaaa tgaaagacag ctcaaaaata gcctatatgc
     8701 tttttaaaaa tgaatttcgt agagccttac atgaaaaatg gatcgacgat gatggtctat
     8761 tatatttgga atttacacaa gcagacctaa tggacaaatt aaattgctac caaggcaaag
     8821 taaaaaccat tttaaacgag ttagaaacat ataatttgat ttcagtcgta aaaggagaat
     8881 ttaacccaaa aacgaaaaaa aatgaaaaaa ataaatacta tttacttcaa cctgaaataa
     8941 gtgccgatga tctttttatt caaaattctt tcgatgacga tttaagcgaa actattgata
     9001 aatcaacctc ttctcggaat gcggaaatcg cacgtcgaga ggaaactgct aaaacccttg
     9061 ataacactgg aaatgcggaa atcgcacgtc gagaaaaaac agctgaaacc cttgataaca
     9121 ctggaaatgc ggaaatcgca caagattatt atattaataa atctttagac actaaaagac
     9181 acttaaaaga cactgaaaca gaccaattac aaaatcaagt tttgctagat aacttcgttg
     9241 aaatcatgaa agaagacagc ataaacacat ttgtaccaga aaaggtatta agcttgataa
     9301 aaacattttc taacacttac gaagaagccc aacaaaccgt caccacaata cacaaagcaa
     9361 aacataaagc cgaggaactt gagaagacgg tagttgtgta tgaagaactt gaatcttacg
     9421 gaattgatgc ggataaagga ttatatatga cgttgctgaa agcttatcaa aaacaaaaga
     9481 ctgagaaagt aaataatttg cagaatttga tttttgtgta tgtaaaaaac tggtttat
//
LOCUS       DACEKH010000096         9496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEKH010000096 DACEKH010000000
VERSION     DACEKH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.100"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            806..1006
                     /locus_tag="IW662_002256"
                     /pseudo
     CDS             806..1006
                     /locus_tag="IW662_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1493..1672
                     /locus_tag="IW662_002257"
     CDS             1493..1672
                     /locus_tag="IW662_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867065.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1718..2323)
                     /locus_tag="IW662_002258"
     CDS             complement(1718..2323)
                     /locus_tag="IW662_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0867066.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2505..2840
                     /locus_tag="IW662_002259"
                     /pseudo
     CDS             2505..2840
                     /locus_tag="IW662_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3005..3232
                     /locus_tag="IW662_002260"
                     /pseudo
     CDS             3005..3232
                     /locus_tag="IW662_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3358..4329
                     /locus_tag="IW662_002261"
     CDS             3358..4329
                     /locus_tag="IW662_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0867067.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4469..5782
                     /locus_tag="IW662_002262"
     CDS             4469..5782
                     /locus_tag="IW662_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0867068.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5825..6691
                     /locus_tag="IW662_002263"
     CDS             5825..6691
                     /locus_tag="IW662_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0867069.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6688..7536
                     /locus_tag="IW662_002264"
     CDS             6688..7536
                     /locus_tag="IW662_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0867070.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7550..9022
                     /locus_tag="IW662_002265"
     CDS             7550..9022
                     /locus_tag="IW662_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ0867071.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9039..>9496
                     /locus_tag="IW662_002266"
     CDS             9039..>9496
                     /locus_tag="IW662_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0867072.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHP"
BASE COUNT         3005 a         1599 c         1957 g         2935 t
ORIGIN      
        1 tctatatttt gatatacaaa ttacagcttc agatactaag ttgcataaac cttttccgga
       61 ccccatacaa aaggcaattg atgaattggg cataaaaaat aagaaacgat ttatattggt
      121 gattcattat atgacatgca aagtgcgaaa gcttgtggga tagcatttgg tttagctaaa
      181 tggggagcca aaaatataga aaattttcaa agtgtagata taattattga ttctcctcaa
      241 gaaatacttg atttgttaga aaaatagaca aagagcgtga gataaggata gggtctcacg
      301 ctctttgttt ttcgccattt aaagattgaa atttttgcca tttaaaaaga aaagtgacag
      361 ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt ctaatactag
      421 gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt gtcagctatt
      481 ctatattaac attgattgct atggttttag gtacttcatt ttataatgct tttgtaagta
      541 agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca agtaattaat
      601 atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca cattaagaac
      661 gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc atatttgttt
      721 gttatgctat acatagatct gcataattag acttgcgctg cccgttattg aagtttcagg
      781 cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga aacgctggca
      841 gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct accccactgt
      901 tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag atatagatac
      961 ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag aaacaagcga
     1021 agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca actctaagct
     1081 cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa tacatggatg
     1141 tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag ttgagtattg
     1201 cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag atagctccat
     1261 ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc tgtataatag
     1321 aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg ttcactatcg
     1381 aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt taactgtggg
     1441 aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg gggtgactga
     1501 actaaccgct gatatgagcg atacttggct gcaggaatat ctttttccgg aaaaacaaac
     1561 aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac taatgaaaaa
     1621 gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat agaaacgaaa
     1681 atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc gctttcttta
     1741 ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca actggaactc
     1801 cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga tctgttgagt
     1861 tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc aataatactc
     1921 cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa cttagcaatg
     1981 ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta aaatctaaaa
     2041 actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta actgtatttt
     2101 ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca caatcacctt
     2161 ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta ccactggctt
     2221 tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt cgatctaaat
     2281 tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact ccttttaaaa
     2341 aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata gcatttttaa
     2401 aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat gaaattaagc
     2461 gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag ttaaataaag
     2521 gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc aaactttatc
     2581 aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta actaatgcta
     2641 tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa acggaagggt
     2701 tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat cacattaatt
     2761 ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata ctacgtccgt
     2821 taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca ttttcgcacg
     2881 gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata ctaataaatc
     2941 aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat gttccatgta
     3001 ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact tagtcgtggt
     3061 gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt aggctgctgg
     3121 aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat tgacaaagta
     3181 tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa atttttttac
     3241 gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa tttggaatcg
     3301 tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt tcgtgaagtg
     3361 aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac ggtatctaga
     3421 gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc tgcaattagt
     3481 gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag tagcaataca
     3541 atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc ctactatgta
     3601 gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa cggcagtgcg
     3661 gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg aattataggt
     3721 attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt cagtatagac
     3781 cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg tgggcaaatt
     3841 gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg gggtgtttca
     3901 ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg taaggaaaat
     3961 aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga ggaacaattg
     4021 ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa tgatttcatg
     4081 gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa ggattttcaa
     4141 attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact atcaacgatt
     4201 gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc gatgatttta
     4261 aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg agagacgact
     4321 aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga aagtgatttc
     4381 aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa ataaaacgga
     4441 aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa ttgcagcaat
     4501 tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa gttcaggtag
     4561 cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc ctgaaggaaa
     4621 agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat ataaagctgt
     4681 tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag taaatgctgc
     4741 aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata ctgcagctta
     4801 tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg acatggatga
     4861 tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg cgtttggttt
     4921 ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg ctggaattac
     4981 agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc aagcaatttg
     5041 ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg cgtcgggaga
     5101 cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg atgtcgttaa
     5161 tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg aagcgttgga
     5221 atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg aaaaaggatt
     5281 tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag atttagagga
     5341 aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg caactgggga
     5401 gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag agaaaaagga
     5461 tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta ttatgagttt
     5521 agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag atgaagtgtc
     5581 ggaaggaatg caggtattaa ttaagcaaaa ccaaatttct ggacatgccc gcccagtggt
     5641 tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata ttagctactt
     5701 cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa tgcaacaggc
     5761 aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt taaaaaaacc
     5821 gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc tgctgctgac
     5881 gatttttctg atcatacccg taatcatggt aatttattat gcctttactg attattattt
     5941 gttaatgcca gatgctagac aatttatcgg cttagaaaat tttaagcgac tgttcgctga
     6001 tccaattttt gtaaaaagta tttggaacac ggctaaattt gttgtttgga ttattccggt
     6061 ccaaataggg gcagctttag gaatggcact tattatcaac aaaaatcgta aaggaaatat
     6121 cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta tttcgatttt
     6181 atggttgtac ttgttaaatc ctaatgaagg tctggttaat acgctatttg caaagattgg
     6241 aattgcttcg cagccttttt tgactagtcc caaacaagca atgtatacgc ttgtgttcgt
     6301 atcggcatgg caaggtgcag gatatcagat gctgctgttt ttagggggaa tgcaaaatat
     6361 tccgcaagac gtttatgagg cagccgaaat tgatggtttt tctaagtttc agcaatttcg
     6421 ctatatcacc atgccattat taaagcccac ggcgattttt attttactta ccacattgat
     6481 ttcggccttt aaactaatca ttcagccaat ggtaatgact caaggtggac caatgaattc
     6541 tactatgaca atggtttatt acatttacca aactggattt acagaccgta tggtaggcta
     6601 ctccagctcc attgcgttaa tctttaccac tatgattggt ttaattacaa ttgctcaaag
     6661 aaaattggta aaggaggatg atccagcatg accaagaaat ttggaccgct aatgatcgta
     6721 gaatattgtt tgctgttcct tttagcggca ttattcattt tcccgatgct gtggatggta
     6781 gtttcctcta tgaagcctga agcagatgtc tatcaaaatt tagcttcctt caaagctttt
     6841 ttacctagtt tgaatccagc taattggttt aaaacttatc aagaggttat ttcccggttc
     6901 agtgtgggaa cttatttatt aaacagtatt ttttatggtt taacctttgc ctgtgggtca
     6961 atcattgtaa actctttggc gggttttgcc tttgcgaagg ttaatttttc aggcaagaaa
     7021 attatctttg gagctttgtt agctctgctg attgtcccga tggagacggt tttaattccg
     7081 caatttacca tcgttaattc actaggctta gtgaatactc gcttggcagt gattttacca
     7141 gcgatggcca gcgcgtttaa tatctatctg tttagaaatt tctttatcgc gattccagaa
     7201 gaaatcattg aatcagcgaa attagatggt gcaaatattg ttaaaatttt ctttcacatc
     7261 atgctgccaa tgtctaaacc agccatagcc acagtaggtg tcttatcctt tatctccagc
     7321 tggaatgatt atatttggcc gttaatggtc ttaactgaca gcagcaaatt ttcaatgcaa
     7381 gtggcaatta ctacgatcaa tactacacag ccagtttata tcaatcaagt gatggctgtt
     7441 ttaaccattg caacgattcc actgattatt gtttatatcg ttgcacaaaa atatattctg
     7501 cagggacttg gcggttctgg aacgggtata aaataggagt gaatagtgaa tgttgcaaaa
     7561 acttttaaag gccaatgagt ttattgagaa aaatgctcaa acagtcaatc aagaataccg
     7621 gaataaattt catttgatgg ctccgattgg ctggatgaat gatccaaatg gttttgttta
     7681 ttatcaagag gagtatcact tgttttatca atattatcct tacgacagca tctggggacc
     7741 aatgcattgg ggccatgcta aaagcaagga tctaattcac tgggagcatt tgccagtagc
     7801 actggcacca ggtgaaagct atgacaagga aggctgtttt tccggcagtg ccatagaaaa
     7861 ggatggcaag ctgtatttga tttatacagg acacaatgtt gtggaagggc aagttcgaca
     7921 agttcagtgt ttggcagttt cagaagatgg cattcacttt gaaaaatatg ccggaaatcc
     7981 aatcatcgca gaagatcact tagcttctgt tgcaacgacc gaagatttcc gcgatcccaa
     8041 agtgtttcag cgaaaagaag actattttac tgtagtcgct actaaaactg cagatgctcg
     8101 tggacaaatt gtgatgttcc attccaaaga tttactaact tgggagtttt attccgtatt
     8161 gctggaggga actccagaac aaggcattgt atgggaatgc ccagacttgt ttgaactaga
     8221 tggcaaagaa gtcttgattc tttctcctat tcaaatgact ccacaaggca atgcttacca
     8281 aaatacgaac tcaactgttg ctttcatcgg tactgtcgat tggcaaacag gcaaatttct
     8341 agtagagaat tttcatgaaa tcgatggcgg tttagatttt tatgcacccc agacctgtga
     8401 aaacgaagca ggccaaagaa taatgattgc ttggatgcaa atgtggcagc gtaaaatacc
     8461 tacgaatgat ctgggccatg gttggtcagg ttccatgacc ttgcctagag agctgagggt
     8521 ggctgataat caattgttgc aacggccagt ttcatcaatt tatcaagtaa ttgatccgca
     8581 ggaaaggatt gaatgggcga agactgaaga gaatcgcttc ttactgaagg acttagtata
     8641 tgaccagcac tatttacgct tggaaatcaa tttaaaacag gcaacgaaac tagaacttca
     8701 ctatgcgaag ggacccgacc atacattact gcttaactat gatgttgaac gggagtgctt
     8761 tactgcttcc agggaacaca tgccgtatga tttatcagga gctgaaaagg aagtgttgaa
     8821 aaagcgcagt gtgacgattc ctttggaaaa taattgtcta accatcgaaa ttttccgtga
     8881 tacttcagct attgagcttt ttttaaatga tcgtctgact atgagtacaa ctttttatga
     8941 gacagaaaaa ggacaggatt ttcttttgct ggcagacagt gaagtaacct tttcattgga
     9001 aagcggctta attaatctat agaagggaga gctcagtgat gatttatggt tcaatcgaag
     9061 caggcggcac aaaatttgtg tgtgccattg gcgatgaaga aatgacgatc aaggaaagag
     9121 tgagctttcc gacaacgaca cctgaggaaa caataccttt agtcattgac ttcttcaaac
     9181 aataccaagc agatttagca gggattggga ttggctcatt tgggcctatc gatatccatc
     9241 gcgattccgc cacttatggg tacattacct cgaccccgaa attggcatgg caaaattttg
     9301 atttcatcgg taccatgaaa aaagaatttc cgatcccgat ttcttggaca acagatgtca
     9361 atgcggctgc ctatggtgag tatgtcttcg gcagtggcaa aggattgagc agtgtcgttt
     9421 actatacgat cggtacgggt gttggcggcg gtgccttaca agaagggcgc tttatcgaag
     9481 ggtttagtca tccaga
//
LOCUS       DACEKH010000097         9118 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEKH010000097 DACEKH010000000
VERSION     DACEKH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.101"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            27..1214
                     /locus_tag="IW662_002267"
     CDS             27..1214
                     /locus_tag="IW662_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAQ0867073.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            1195..2499
                     /locus_tag="IW662_002268"
     CDS             1195..2499
                     /locus_tag="IW662_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0867074.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2525..3538
                     /gene="citC"
                     /locus_tag="IW662_002269"
     CDS             2525..3538
                     /gene="citC"
                     /locus_tag="IW662_002269"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAQ0867075.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3542..3835
                     /gene="citD"
                     /locus_tag="IW662_002270"
     CDS             3542..3835
                     /gene="citD"
                     /locus_tag="IW662_002270"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAQ0867076.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3840..4745
                     /gene="citE"
                     /locus_tag="IW662_002271"
     CDS             3840..4745
                     /gene="citE"
                     /locus_tag="IW662_002271"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAQ0867077.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4738..6297
                     /gene="citF"
                     /locus_tag="IW662_002272"
     CDS             4738..6297
                     /gene="citF"
                     /locus_tag="IW662_002272"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAQ0867078.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6294..6851
                     /gene="citX"
                     /locus_tag="IW662_002273"
     CDS             6294..6851
                     /gene="citX"
                     /locus_tag="IW662_002273"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAQ0867079.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            6844..7707
                     /gene="citG"
                     /locus_tag="IW662_002274"
     CDS             6844..7707
                     /gene="citG"
                     /locus_tag="IW662_002274"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAQ0867080.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            7941..8350
                     /gene="tnpA"
                     /locus_tag="IW662_002275"
                     /pseudo
     CDS             7941..8350
                     /gene="tnpA"
                     /locus_tag="IW662_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(8987..>9118)
                     /locus_tag="IW662_002276"
     CDS             complement(8987..>9118)
                     /locus_tag="IW662_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0867081.1"
                     /translation="RRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         3180 a         1420 c         1849 g         2669 t
ORIGIN      
        1 taaaaaatgt aaaaaggagt gaaggtatgc ttgatgaagt tttagaaatc cataaaaaaa
       61 atgtaggtgt tttacggatg gaaacaacag aagccgtgca agatgatcaa gatttagcaa
      121 aaatgtacac cccaggtgta gcagaactaa gtttaatgat tgagaaaaat catgctttgg
      181 caagagaatt aacaattagt ggtaaattag tggccgttat tacagacggg tcggcggtat
      241 taggtcttgg aaatgtagga aaccaagctg gattaccaat cgtcgaaggg aaagcgctgc
      301 tttataaaaa tttagcaggt gtcaatgcga ttccattagc catcgaacaa aaatcagtag
      361 acgagattgt acaaacaatt gtcaatttgc aaaatagttt tgcagggatc catttagaag
      421 atattgcagc accgaagtgt tttgagattg aagaaaaatt acaagaaaaa ctttctattc
      481 ctgtgtatca tgacgatcaa gaaggaacag cgatagttgt tttagctgga ttgatcaatg
      541 ctgctaagat ccaaaggaaa acattaactg aacttcgagt tttgatcaac ggcatgggag
      601 catctggggt tgcaacagct agactattaa tcgctgctgg tatcaaaaat ctaacattgg
      661 tggataaaca agggagatta aagaagcaag atagtacatt aaataaatat caaagagagt
      721 tgttggatca tatacatgat acaaataata atgcaagcgt tttggaagat gcaatcgttg
      781 gacaagatgt ttttatcggt ctttcaatag gaaatgtttt aacgcctgaa atgattggaa
      841 aaatgaatga taatccgatt atttttgctt tagccaatcc agtaccagag atcacacccg
      901 atttagcaaa agaatctggt gcaaagctaa tcgctacagg ttcttcgaaa tatcctaacc
      961 aagtaaacaa tattttagta tttcccggat tgttcaaggg actattacta tcgaaagcga
     1021 ataaagtcga tctttcatta caacttgaag tagccaaaca attagctgct aaggtcgcag
     1081 agcctacggt agaaaaattc atccctaatg tttttgatga aggagtcgca aacgtcgttt
     1141 cacaggcagt tttggattat ttacaaaaac taaaagaaaa cgaggataat atacatgctg
     1201 aaaacattaa atagaattaa aattggtttt attcctttat ctctttatat cattttggta
     1261 ctcgcgtatc ttgcaatgac gataagcaat atcgttccag aaaatgatat gttagtttct
     1321 ttgggtatta tgactgtttt tgcttattta ttagaagaaa ttggtaaaac tattcctatt
     1381 ttaaaagatt taggtggtaa agtattagtt gttacatttt tgcctgcatt tctagtctat
     1441 aaacattggc tacctcaaag tactgtaact gtggtttctg attttatgaa taataacaat
     1501 ttcttgatgt tctttatcac actactagtt gtcggcagta ttgcttcaat gaatcggcaa
     1561 acattggtta aagcaacttc acgtattatc attattttaa ttttatgcga tgtgattggt
     1621 tctttgatag gaacaggtgt gggaatactc cttgggctat ctgcttggaa tgcgtatttc
     1681 tttatcgttg cgccgattat ggctggtggt gttggggaag gagcgatgcc attatcgatt
     1741 ggttatgcag caatattatc tggtatggga caaaatgata tctttgggaa gattctaccg
     1801 tgtgtgatgt tgggaagttt agtcgcagtt ctactgtctg gtatgctcaa aaaaattgga
     1861 gagaaatacc cacaattaaa tggtcagggt aatttgatcg atggggatga tgaacattta
     1921 gagaacttga agggttcagc taaaccagtc aatattgaaa aaacattgtt agcaggcgtg
     1981 gtagcaataa cgatttattt tgttgctgtc tatgttgagc acttattaaa atcactcctt
     2041 ggcttttcta ttccttcacc tgtgatctta ttagtctctc ttatgggggc taaaatgctt
     2101 ggttttatcc ctaaaaatat cgaagaaggt ggaaattcgt tgtatggatt cactgttcgt
     2161 gggatcacac ctcccttact ttttggcgta ggggttgcaa aaacagattg gttagagctg
     2221 atcgaagtat ttaaaaatcc tgcaacgata gtaacaatca ttgtgacggt agtagctatt
     2281 gtcgctacag cttttatcgc cgcaaaattt ttaaaaatgt acccagttga tactgccatc
     2341 gcagtttcat gttgtagcgg acaaggcggt acaggtgctt tagctatctt agctgcgggt
     2401 gatcgcatgg aattaatgcc atttgcacaa gttgctgttc gactaggggg tgctatgacc
     2461 gtaacgttcg ctatcttttt aatgggtctg ctatcataag aacataaaga aaggacgtga
     2521 ccatgtgttt gatcaaacag atatccaacg attattttta aaaaataact atatcaaaga
     2581 ccaatgggaa aatcttttgc ttgattcagg aatacaaaag aagcaaatcg aagattttca
     2641 acatttagat caaacattgg gtatatacca taaagaaaag ttagtaggaa cagtatctta
     2701 tcaaaacaac gtgattaaat atatcgccgt gtctgaaaaa tataaagatg gtggacatac
     2761 atttaatgta ctagtctctg caataattca ggcaatgagt caacaagaga tttttcatta
     2821 tattgttttc acgaaaccgc gctatcaaaa aagttttaac tttttaggtt ttgaaacaat
     2881 cgtatctacc ccaaaaatag ctgtattaga aatgggagat agttctataa aacagtacct
     2941 agagaggatt cctaaagtga atggtgaaaa aaaagcagca attgtcatga atgcgaaccc
     3001 atttacagaa gggcattatt atttagtaaa acaagctgcc aataacaacg attgggtata
     3061 tgtatttgta ctcgaaaaag aacaaaatct tttttcgacg aaggaccgac tcaaattagt
     3121 tcaacaagga ttacaaaaac tgaaaaatgt cattgttcta tcaggtggcg agtatattat
     3181 tagccctgca acgtttccca cctatttttt acgagaagat gatgaaatag caaaggagca
     3241 aatggtcgta gatgctactt tattcaaaga aagaattgcc acttatttag atatctctgc
     3301 tagatatgtg ggagaagaac cactttcccc aatgactcgt tcatataacg acatactgga
     3361 aacgatttta ccccctgaaa taaaagttca tgtccttgca aggaagaaaa cggaacaaca
     3421 ccaagttatt tctgcttctc aagttcgtaa agcgtatctt gctgggcaat tggaaaaaat
     3481 aaaatatatg gttccagaaa caacctatca atacttaaaa aataaaaggg agcgttaagt
     3541 aatggaaata aataaatcag cgattgctgg aacattagag tcaagtgatg tccaaatcat
     3601 gatttcaccg aatacagaag gaatagaaat cgatttagac agtcaagtga ttgagcaatt
     3661 tggaaaacaa atcaaaaaag ttatctatga cacactagca gtttatcaga ttaaaaacgt
     3721 aaaactcaag gtagtagata aaggggcatt agattgcgtt attgttgcac gattaaaaac
     3781 agcgatccat cgttccttag gtatccaaca ccaagaaaca ccgtgggagg tttaattaga
     3841 tgatgaaaga acgtttgaga agaacaatga tgtttgttcc aggaaataat ccagcgatga
     3901 tcaaagatgc aggtatttat ggtgctgact cgattatgtt cgacctggaa gatgcagtgt
     3961 ctatggcaga aaaagacagc gcaagaatgc ttgtggctga ggcattgaaa acagtggatt
     4021 atggtgacac agaattaatt gttcgaataa atgacctgca ctcagaattt ggacgtgcag
     4081 acatttttgc aatggttaaa gccggcgcgc atgtattacg tttacctaaa acagaaacct
     4141 ctgaagatat aacagaagta gaaaaagtga ttatagaagc agaaaactat cataatgttc
     4201 cgatagggcg aacattgatg atggcagcca ttgaaggtgc ttctggtgta ttgaacgcac
     4261 ctgcgatcgc caaagcatct gatcgattga ttggaatcgc cattggggca gaagactatg
     4321 tgacaaatat gaaaaccaaa cgatatccag atggaaacga attgtttttt gctcgcagta
     4381 tgatcgttca tgctgcacgt gcagcaggca tttccgcatt tgacactgtt tactctgatg
     4441 tggatgatat ggaaggattt ttaaacgaag tacgtttaat ccatcaatta ggttttgatg
     4501 gaaaatcttg tatcaatcca cggcaaattc cagtagtcaa tactgtttat cagccgtcaa
     4561 tcaaagaaat caaacgatca ctagacatca tctcggcaat cgaagaagca aaagaaaaag
     4621 gttctggcgt aatttcttta aatggaaaaa tgatcgataa accaattgta gaacgtgcac
     4681 aacggacatt gatgttagca aaagcatcac gattgattga tgaggaggga aatatccatg
     4741 cataataaac aaagacgtag cattccatca agtgttttaa caaagtataa aaaagaattc
     4801 caaagtgttt cattatcgcc acaaacgatt caacgttgtg caccaaaagt aacgactgta
     4861 actaatcaga caaataaatt gatgcactcg atcaaagaag ctatagaaaa atcgggctta
     4921 aaagatggaa tgacgatttc atttcatcat catttgagag aaggtgattt tgtttttaac
     4981 caagtgatgg ccgtgatccg tgaaatgggc atcaaagaat tgaccttagc accaagttct
     5041 ttaacgaaca ctatgaatga gatggttatt gaatgcatca aggacggtac agtaactcat
     5101 atcacaagca gtgggatgcg cggcagctta ggtgaattta tttctcatgg aggaatggac
     5161 aatcctgtga ttttacgttc tcatggtaat cgtccacgtg caattgaaag tggtgaattg
     5221 aaaatcgatg ttgcatttct aggagtacca acatctgatg attatggaaa tgctaatgcg
     5281 actgttggaa aatccgtttt tggttcatta gggtatgcaa aagtagatgc acaatatgca
     5341 gataaagtca ttcttctaac tgatcattta gttccttacc ctaatacgcc cattagtatt
     5401 ccgcaaacac aagtcgacta tgtcgtggaa gtagaagcaa ttggtgatcc gaataaaatc
     5461 ggtgctggtg ctacacggtt tactaaaaac ccaaaagaat taaaaattgc tgaaatggtc
     5521 aaagatgtca ttgttcactc accttatttc aaagatggct tttcttttca aacaggaacg
     5581 ggtggtgcag ctttagcagt aacgcgttac ttagctagtg aaatggaaga aaaacagatc
     5641 aaagcttctt ttgcattagg tggcatcact aaaccaacgg ttgatttatt ggaaaaaggt
     5701 cttgttgaaa aagtgttaga tgtccaagac tttgacaaag gagcagctga aggaatggcg
     5761 cggcttgaga accaacagga gatcgatgct tccttttatg ctgatccaag taataaagga
     5821 gcggttgtta accaattaga tgtcgctatt ttatctgcat tagaaattga cacaaacttc
     5881 aatgtaaatg ttatgactgg ttcagatggt gtgctaagag gtgcgattgg tggacatcaa
     5941 gatgcagcaa cggctaaact tactatcatc tcagcgccgt tagtacgtgg ccgaatacct
     6001 acagtagtaa atgatgttac tacattgatc acaccaggga aaagtatcga tgtattagtc
     6061 acagaagtag gggttgctat caatccccaa cgtaaagatc taatagctat ttttgaaaga
     6121 attccacaaa ttcctgtttt tacaatagaa gaattacagc aaaaagcaga aaaaatagta
     6181 ggcatcccgg aacctttgca attcacagat cggatagttg cctatgtaga ataccgagat
     6241 ggttcaattt tagatgtcgt tcgtcaggtg aaagaatttg aagaagagag atcatgatgt
     6301 ttgatactgg agaaattcaa gaaatcgagg atattttgat ccataaagaa caacgtgcta
     6361 acttgcaaag caacctgctt tccaagtatt caaatagttc attgatggtc tttacattga
     6421 atattcctgg gccgatcaaa aataatccaa tgattgagca ggtatttcgt cagggacagc
     6481 agtcaattca gcaaatgttt ttaaaaaaaa aggtggaaat gacgtacaca aaagaaatca
     6541 atttgccaag tggattggac tggtacgtaa tagtaacaga agcacctttg aatctcaaaa
     6601 aatggttggt cacgttagaa gagcaagacg cattaggtag actttttgat ttagatgtct
     6661 tgttcaaaaa aggagagcag ataacaattg tcagtcgcca agctcttaat catggggaac
     6721 gtccttgttt tatttgtggg aaatcagcaa aggtatgtgc acgtaatcgt acacatactg
     6781 tgaaagaaat gcaaaagaaa atcgaacaaa tagtagccga ttccttaaaa ggagcattga
     6841 aacatgcatg ataatattca aaaaatagca caagaagcac aattagcgat gctttatgaa
     6901 gttacatgta tcaataaacc aggattggtt gatccagtag accccgggtc tcatcaagat
     6961 atggacattt ttacttttct acaaagtagt gtagttttga ctccctattt tgaagaattt
     7021 atccaaacag gattggaccg acaacatcaa ccaattgaag aaacctttat ggcgattcga
     7081 cagattggtt tagaggctga aactgcgatg ttctcagtga caaatggtat aaatacacat
     7141 aaaggtgcta tcttttcatt ggggattttt cttgctattt gtggccggtt aactatttgg
     7201 aaagttcctt gtaaaaaaag tttctttcaa aaagaaattc aaaaaatgac gaaaaatcta
     7261 cttaacgatt ttgggaagat tgatcaaaca aaaccaaaag catggacttg gggagaatat
     7321 ctatttgtta accatggatt gacagggata cgaggggaag ccaaaagagg ttatccttgt
     7381 gtttttgacc gaggattgcc ttattatcaa gcatatcaag ggtcgcaaca agataagatg
     7441 atagacactt tactttttct atcaatggaa gtagaagata ccaatttgat caaacgaagt
     7501 aataatagac atgtttttga agagtatcaa ttacttattc gtccttattt tgaattaggg
     7561 ggtgtaaaaa ccatacaagg taaaaaatat ttggattacc taaatcagca atttaaaaag
     7621 aaaaattgga gtattggtgg aagtgcagac ttattgatcc ttacaatctt tttagataaa
     7681 atcatggaca aaggatggtt atgttgatac tataatacat tattttgtat tagtaagcta
     7741 ataactaaaa aatggttcaa tacaaatatt ttataaaaaa tggtagtgcc tatcctttca
     7801 gattgtagac aaagaaaaat ttctttgtct acaatttttt tgttaatgac ttcagtcagt
     7861 ttcgtgcgca aagcgtgcga aacaagaaaa ccatgcgttt ctacacgtgg aagaactagc
     7921 atatcgaaag gagttttttc atgtcaaatg acgataaaag tttagcccat actcgttgga
     7981 attttaagta tcatattgta tttacaccaa aatatcgacg aaaagtaatc tataggcaat
     8041 tacgaaaaga tattggaaaa atacttagga agttgtgcaa aataaaaaac gtggaaatta
     8101 tagaagcaca tgcaacgcca gatcatattc acatgcttgt tcaagtacca tcaaaaataa
     8161 gtatatccag ttttatagtt atctgaaaga tcgtagtgct gtgctaatcc acgagcagca
     8221 cgtaaatcac aaatatagat acggaaataa atcattttgg tcggaggaat attatgtgag
     8281 tacagtcgaa ttgaatcaaa agacaattga aaaatacatc cgtgaacaag aagctgatga
     8341 tcgcatttga gatagtataa gtaaaagaga gtagatgttg caataacaaa agggtaaaaa
     8401 tggactacct ccagtaaaat accgaaaaat agtttaactt ttggagataa gtccatttaa
     8461 gggtctatag aatgacaatt tttttagggg attttactat tattacttca agacccgctt
     8521 ggattcgcaa agtgagtgaa ctcttaaaac tacctgatag taggttgttt taagagttat
     8581 ctgaaagtaa aaattgcatt tatatttttg ataaatacgt aaatttatat atacgtattt
     8641 atcaaagttt taggccaaag gaatattgtg taaatacact aggattgaat gaatagacca
     8701 gaagaaattg cgtccaagaa caaaagaagc atgatagtgc tctatataaa ttgggcattc
     8761 aagaatctga aatttaaagg tagaatttta gttagacttt tagattttaa acacaaaaat
     8821 aaccatacaa gcaacaagaa gcaaagtgct atattataaa ttgtccgtcc gtttacttac
     8881 ataagatcat tggggctatt tttaaaagag aataatagat aataaatgaa aaaaaagtca
     8941 agtccacaat cctgtgtaaa atactataca atgctctcac tattttttac ttggcaaatt
     9001 taatatattt tcttgatgaa gaaagccact catcgtgttg atcaataagg actgctccaa
     9061 ttaaacgatt ggctgaaacg cagtttggaa agatacgaat aattttttct cttctgcg
//
LOCUS       DACEKH010000098         8994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEKH010000098 DACEKH010000000
VERSION     DACEKH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.102"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            112..930
                     /gene="purR"
                     /locus_tag="IW662_002277"
     CDS             112..930
                     /gene="purR"
                     /locus_tag="IW662_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ0867082.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1433..2806
                     /gene="glmU"
                     /locus_tag="IW662_002278"
     CDS             1433..2806
                     /gene="glmU"
                     /locus_tag="IW662_002278"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ0867083.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2900..3871
                     /locus_tag="IW662_002279"
     CDS             2900..3871
                     /locus_tag="IW662_002279"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0867084.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3877..3979
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4045..4614
                     /gene="thiT"
                     /locus_tag="IW662_002280"
     CDS             4045..4614
                     /gene="thiT"
                     /locus_tag="IW662_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ0867085.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4695..5468)
                     /locus_tag="IW662_002281"
     CDS             complement(4695..5468)
                     /locus_tag="IW662_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867086.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5735..7435
                     /locus_tag="IW662_002282"
     CDS             5735..7435
                     /locus_tag="IW662_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ0867087.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7405..7623
                     /locus_tag="IW662_002283"
     CDS             7405..7623
                     /locus_tag="IW662_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867088.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7643..8533
                     /locus_tag="IW662_002284"
     CDS             7643..8533
                     /locus_tag="IW662_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ0867089.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8551..8835
                     /locus_tag="IW662_002285"
     CDS             8551..8835
                     /locus_tag="IW662_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867090.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         3035 a         1537 c         1888 g         2534 t
ORIGIN      
        1 attttaataa ataccgaact attttcttat tttttcataa aatgctcgaa tttttgaaga
       61 aaaacttata taatgtaaaa cgataaaatt tcttaaaagg agttggaata ggtgaaagta
      121 cgtcgcagtg aacgtctagt tgacatgaca cagtatttat tgaatcaccc gcatgaattg
      181 atttccttaa caagctttgc cgaacgttat gaatctgcta aatcttctat cagtgaagat
      241 ttgggaatca tcaagaaaac atttaaagaa cgagggtatg gaaccttgga aacaatcccg
      301 ggtgctgcag gtggtgtatt gttcatccct gaaatttcat atgaagaggc gaaaaaatat
      361 attgaatctc tagcagaacg tctatcagaa caagaccgtc tgcttccagg aggatatgtt
      421 tatctctctg atttgttagg agaacctgaa ctgctgcgtc aagttggaag aatcattgct
      481 tctaaatacc ttggtgaaaa aatcgatgct gtaatgactg tagccacaaa aggcgtacct
      541 attgcccaag ctgtgtccta ttatctgaat gcgccattcg ttattgtacg ccgggactca
      601 aaaataacag aaggatcaac agtaagtgtc aactatgtgt ctggatcttc tgaaagaatt
      661 gaaaagatgg aactgtctaa gagaagtttg aaacgtggtt cacgtgtcct tgtagtggat
      721 gatttcatga aaggcggcgg aacagtcaac ggaatgaaga gcttgattga agaattcgaa
      781 tcagaacttg ttggcatcac tgtatttgcc gaatcaaaat tcaacggtca tcgcgccatc
      841 gatgattata cgtcactgct ttatgtcaaa gatgtagata ctcaaacgaa aaatattacg
      901 gttgtgccag gaaattattt ttcagaataa gaggttgtga aaaagctgct tagtttcgag
      961 tagtaagaaa aattttgtga aaatggcttt ccacattttt tcataaattt ggcttaatac
     1021 cgagaaactg gcttttgaac accgtttatc tagaggttgt gaaatcagcg tcctgtcctg
     1081 agtattagct caataaacgg aaaaattgct cctcatattt tgccgtttat tgagctaata
     1141 ccgaaggtca gctgattgaa aaacgtttaa tgaagaggtt gtaagcctac cgtttaactc
     1201 taagtcacaa gaaacgattt caaaaaacag cttttcatgt tttatgagta gtgtgacttg
     1261 tgaccgagga gttggcagga gagcaccggt taccaaatag ataggcccgt tatggtctat
     1321 ctattttttt atccagaaaa cgctgggata aaagaaaatc acttgttttg ttgaaaaaat
     1381 gggtatactg ggataaactg aaatcagcaa actatattta ggagtgacgc agttggacgc
     1441 acgttatgca attattttgg cggcagggaa aggtacccgc atgaagtcaa aattatataa
     1501 agttttacat cccgtttctg gtaaacctat ggtagaacat atcattaatc gggtcagtga
     1561 aacgaaacca gatgaagtga ttacgatcgt tggacacgga gcagaacaag ttaaagcaca
     1621 actaggagaa cgcagtaaat atgcgttaca agcagaacaa ttgggaacag gacatgctgt
     1681 tttgcaagct gcttctttct tggaaggaaa aaaaggaaca acgttagtta tcagtggcga
     1741 tacaccatta ttgactactg aaacgctgaa caatcttttt gaatatcacc aaggaaaaaa
     1801 tgcaagtgca acgatcttga cggcacaagc agaaaaccca acaggatatg gacgaatcat
     1861 ccgtgaccat attgggatcg tagaaaaaat cgtggaacaa aaagatgcaa caccagaaga
     1921 agctcttgta caagaaatca atacaggaac ttactgtttt gacaacgaag ctttgttcga
     1981 tgcgttaagc aaagttggaa caaacaatgc tcaaggtgaa tactatctaa cagatatcat
     2041 cgaaatctta aaagaagaag gccatacagt tgctgcttat caaacggatg attttgaaga
     2101 atcaatggga gtcaatgatc ggatcgcgtt agctaaagca aatgagatca tgagaaagcg
     2161 aattaaccag atgcatatgg tcaatggcgt aagctttgtc gattcagcaa cgacatacat
     2221 cgatgcaggc gtagaaatcg gtccagacac attgatcgaa gcaggtgtac aaatccaagg
     2281 aaatacagtc attggttctg attgtgtcat cgggtcacat tcaaaaatcg tagacagtag
     2341 gattgaggat cacgtcgtca ttgaaaactc agtcattgaa agcagccatg tcaagaaaca
     2401 tgcagatgtg ggaccttatg cacatttacg accaaaagct gaaattggcg aaaacgtcca
     2461 tatcggaaac tttgtggaag tgaaaaatgc tcaaatcggc aaaggaacta aagtcggtca
     2521 cttgacttat gttggtgatg ctacgctggg agaagaaatc aatgtcggat gtggtgttgt
     2581 cttcgtcaac tatgatggaa agaacaaaca tcatacgact gtcggcgacc acagctttat
     2641 tggttctagc acgaatatca tcggacctgt cgaggtagct aaaaacagtt cgattgctgc
     2701 cgggtcaaca attacagaca atattccaga atatgcgctg gcaattgcga gagcaagaca
     2761 agtcaataaa gaaggatatg caaaaaaact cccttatcta aactaagact tttgcaaaat
     2821 cacttgattt atttaaaaaa tatgactact atttattagg tagaattcta gagttgtgaa
     2881 aaagaaagtg gaggtcccca tgtcaaaaca ttactttgac ccaagattaa aaatttttgc
     2941 gttaaattcc aatcgcccat tagcagagaa aattgcagca gcagtcggcg tcgagttagg
     3001 aaaatcatct gttactcaat ttagtgatgg agaaattcaa gtaaacattg aagaaagtat
     3061 tcgtggatcg catgtctatg ttattcaatc aacgagcagc ccagtcaatg ataacttgat
     3121 ggagttgctg atcatgattg acgcactgaa acgagcaagt gcaaaaacaa tcaacgtagt
     3181 catgccttat tatggctatg cgcgtcaaga ccgtaaagca cgtgcacggg aaccaatcac
     3241 agcgaaatta gtagcaaaca tgattcaaaa agctggtgca acaagaatgc tgactttgga
     3301 ccttcatgca gttcaaatcc aaggtttctt tgatattcca gtcgatcatt tgatgggtgc
     3361 gccacttatt gccaattatt tcttagaaaa aggaatcaaa ggcgatgatg ttgtcgttgt
     3421 ttcaccagac catggtggtg tcacacgggc acgtaaatta gcggaatttt tgaaatctcc
     3481 gattgcgatc atagacaaac gccgtccaaa agcaaatgtt gcagaagtaa tgaatatcat
     3541 tggacaagtc aaaggcaaaa cttgtgtact gatcgatgat atgatcgata cagcaggtac
     3601 gatcacattg gctgccaatg ctttgaaaga agctggtgca actagtgtgt atgcttcatg
     3661 tacacatcca gtattatccg gccctgcttt acaaagaata gaagattcag ctatcgagcg
     3721 tctagttgtt acagactcga tctatcttcc agaagatcgt aaaatcgaca aaatcgatga
     3781 agtaagtgtt ggcggactaa tgggagatgc aatcaaacgt atccatgaaa acaaaccagt
     3841 tagtccattg tttgaaacaa aaaataaata ataaataaac tcacaaaggg gagtccaatt
     3901 gggctgagat tgaatctatt tctaaaccct tcgtacctgt atcggttatg cgagcgtagg
     3961 aattgtgaat aaacagctgt ttgactattt tcgaaagctg cttcctcctt tgtgactttt
     4021 ttattcaaag gaggaagttt ttttatggga aaaaatagag tttgggtaga aggaacgatt
     4081 gttgcagcgt tagcgatggt gttgtcgttg attccgatcc agatcggcag cagtttttct
     4141 atttcattag ggcagattcc attgactctt tttgcgttaa gaagagggac aaaggcaggg
     4201 atgctggcag gtttcatttg gggcatccta cattttccgt tgggacaagt ttattattta
     4261 agtgtcgttc aagtgttgat tgagtacccg attgcctata cttttgcagg cgcagcaggg
     4321 ctcatggctt caaatgtcca aaaaggctat gtagacgata atacgaaaca aattagaaaa
     4381 agcatcgtgt ttggtgcatt gattggcgca ctttccagat atttttggca ttttattgca
     4441 ggagtggttt tctggggagc gtatgcacta tgggggatga atccttggct cttttctttc
     4501 gtaatgaatg gtgccagcgg gttgagtaca gctattgtaa cgatcatcgt tttattaggg
     4561 atccaacaaa cgactccttc gatttttgtg ccagataaac gacaggagct gtaaataaaa
     4621 aagaagcaag aaacttgaga cagaatcatg tcctagtttc ttgtttcctt tttatttttt
     4681 gataagaaat agatctactt caatggcaga taagaaagat cggcatcttc taccaattgc
     4741 cagtgtcgat cggtgacaga gtagattaaa gtagcgatga tttcctcttc tcgataccgg
     4801 gtgcctttta atttccaaat ataatcttca atcacataga ttgggatacg atatttacca
     4861 tcgatcaaac caacttgatc aaaagaaaaa atgatccctt cctgcatcaa attacgcatt
     4921 tctttcaata attccacact gattttttca acaggtgttt cttttttgaa aaaagatttt
     4981 tttacattga tcttatctga caagatcgaa cttaatttac tagcaaaaat cctataaaat
     5041 tcatctaaat agcgataata aatccgattc agctgatcat taagatcgaa gtatacaaaa
     5101 ttattctgta gtttataaaa aaacggcgaa tgaagatgtg ttttcatatg accaaaatat
     5161 aataattctg aaatttctaa aggtgtcaat tctttcagca tcgttaaatc agtaaagtcg
     5221 atccacttca attcatcgaa tccacgcctg ttacgagaag aggaaaagaa acgctgaata
     5281 ttttctgtac cacggattac tttcagccct gtatgggatt catattctcc agcagaagca
     5341 gatggatcaa gcaagagcaa gttttgagga aaatgcacga tactgtttgc aaagtcagta
     5401 gcagtcagcc cttttgttaa aacagcatta cttgtggtat caatatgtac ataaactgac
     5461 tcagtcatca tgttcctcct cttatttcct cttaatctct tttattttac aacatttgca
     5521 ttaaaaaagc ttgtttcttt tattttaaaa ttccattaca aaatcacgtt ttcattgaaa
     5581 acactctcat tttcaattaa tttttgtgat ttttgtattt ttttatttaa aggaggtttt
     5641 atgaattgtt ttcttatgga atctacaaag tcgaatacga gactcatgac ctttttatga
     5701 cgtactggta tacttgaaga aaaaggagag cgcgatggag ataaaaaaag tttacttttc
     5761 acagaaaatg tcgctgtatt cgcctatcca gcaagcaaaa caaccccttc cattttttca
     5821 aagtcatcaa gaaagtagag acgctttttg gcgttctgca aaaaagcaat gtgcttttaa
     5881 agcagtatcg aaaaaatata ccgtctattt tactattgct aaaagtagag gagatgttat
     5941 ttttaataat ctgttgattg aaggtgaaac ttttgaatgg aaaaaatttt ttcggtatat
     6001 ggaagcttgc gctcgttttt ttattaaaga aaactgttgt tttttatttc aatctcctct
     6061 atcagaagaa tggttgcgta tttttcataa aaacggctat caaggaacca cacaattaaa
     6121 taaaaagctg gtctatcata cagcacttgt tttaggtgga ggaggagcgc acggtgctta
     6181 tcagattggt gtgtggcagg cgctgaagga acatgatatc aactttgaga ttatcacagg
     6241 tacctcggta ggtgcgttga acggcgcatt gattttacaa ggagatatga aaaaagctgt
     6301 gaatttatgg aaaaagctgt cgacaagaca agtgcttgcg ttaccagaaa tggctgcagt
     6361 tgaagacctt cgtgaacgct tctatcgaga aagacgccaa atgacgaaaa cagcactaat
     6421 tgaaggcggg gtttcttctg ctccattgga aaagatggtc aaagacaatt tggatttgtc
     6481 cttactcaaa cataatccca aaatacgttt atatacggtg tctactaaac tgccagaact
     6541 agcggaagta gtgactgata tccaacagac aaaaacagaa gaaataccag attggatact
     6601 cgcctctgct tcattttatc ccgctatggc ttatcgaaag atcggaaaaa ataaatatgc
     6661 agacggtggg tatcgcaaca aaatacctat tgatattgcg attaacaaag gagcaacaga
     6721 ggcatttgtc gtagatgtgc aagggccagg cccagcgaaa aaaattcgta tgccagacat
     6781 ttttatccat tggaaatgtc aaacattgtg gacactaggc agctttctgc tttttgatag
     6841 ccaaaggaac caattaaatt tacaattagg ctatctggaa atgaaaaaac gattaggcta
     6901 ttactatggg aactggtata catttcattc tgttaaacct gcaagaacat actggcgagg
     6961 atttttgtct tacttaacaa aagaaattca gttagaatta tcttttttca aaagcataaa
     7021 attttggcaa aaactgcgta atttatataa gaacagggtc gtaccagaga catgcggatt
     7081 ggcgatgcta gaacttttag caaaaaaaca ttttttactg cccaatgaaa tctatcaagt
     7141 agacatgatg attcaaatga tctgccaaca agatataaaa agtatgccgg atgatttatt
     7201 ggcacaaatt ggtcaattaa gtactgaaga atggcgaagg tatcgaaaat accagcaaaa
     7261 aatacaaaat gagcgcacga aaactgttta ttttgattat ctgctacaag gaaaacgcaa
     7321 ggatcgtttg cagcatgaat tgcttaagca accagttgac acattactga tcctatattt
     7381 atattatctc aaggaggaac aaccatggca caagaatttt catatgaaat cgtagaagaa
     7441 atcgctatat tgtcagaaaa caacaagggg tggcgtaaag aactgaacct agtcagctgg
     7501 aatggtcgcc cgccaaaatt cgatcttcga gattgggcgc cagaccatga aaaaatgggg
     7561 aaaggcctga cattgacgaa cgaagagttt gaacaactgc aaaaagcaat agaaaatatg
     7621 taatgaggaa ggaccctgtc gaatgaaaag tatgactggt tttggcaaag ccatccgcga
     7681 aacaaaagcg tatcagttag atattgaaat aaaaagtgtc aaccagcgtt ttctagatgt
     7741 tcaaatacga agtcctaaaa tcctgaactt tgttgaaaac gattttcgtc agcttattaa
     7801 acagcagtta agccgcggtc gaatcgaagt atttgtgaat ctagcttata ttggcaaaaa
     7861 cgaaaaacag atcgtcatca actgggaatt gattgattca cttatcactc aattgacaga
     7921 cggaattcaa caacgatacg gtagtcaaaa tcaagtgtca attagtcgga tggcggaacg
     7981 cttcgcgctg gacgaaaact tcgtaacggt agaagaaaaa ccggaagaga gcatggaaga
     8041 attggcacag ttagctctag atacattgaa aagcgcactt gattctattg aggcgagtcg
     8101 agaaaaagaa ggtcaagctt tagctttagt gttgaaaaag aatactgaag aattcaaaga
     8161 agtactaaaa aaactcgata gttttgtaga aatctatgaa aaggaatttc aaataaggta
     8221 tcaaaaaaag ctggaggaat ttcttggagc gaaagtcgat gagcaacggt tgttgacgga
     8281 gctagccatc ttgttggaac gcggagatat tcacgaggaa ctggatcgtc tggcgatcca
     8341 tgtacaaaaa ctagatgaac ttatcgaagc acaatgtcct gtaggtcggg aattagattt
     8401 cctgatccaa gaaatgaatc gggaaatcaa cacgatcggt tccaaatcaa atgcaatcga
     8461 aattaaaaat caagtcgtcc agtctaagac gatcttagaa aaaatcagag aacaagtaca
     8521 aaatattgaa tgacgaaaga ggtagagata atgaaatatt atgacgtaac gttccacgaa
     8581 ttaagtggaa aaactgtggt taaacgggat atcccttctg aaaaaaacgg ctttgacgta
     8641 tggaaagatg cctgtgcaga ctataatgag aacgaactgt ttattttgat caacgacggt
     8701 gcttacgtga cgatgaatcg gaaatttatt gttcgaattg acacagaaga ggtagaagat
     8761 ccgacagaaa aagcacgttc tcgaaaagat gagatcatgg gtgtagtgaa tactttgtcc
     8821 aatatgggct tttagcaagg atgctgaaca aagaaataca ataaataatc agaattggat
     8881 ttcttcgctt agcttctttg ctctaggaat acatttaaag gttgtgacaa aattcgtgtc
     8941 acaatctttt tttattttga ataagtgatg ttcaaaaacc attttctcgg tatt
//
LOCUS       DACEKH010000099         8942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEKH010000099 DACEKH010000000
VERSION     DACEKH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.103"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(435..932)
                     /locus_tag="IW662_002286"
     CDS             complement(435..932)
                     /locus_tag="IW662_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0867091.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(972..1781)
                     /locus_tag="IW662_002287"
     CDS             complement(972..1781)
                     /locus_tag="IW662_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0867092.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLAFT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2115..2972
                     /locus_tag="IW662_002288"
     CDS             2115..2972
                     /locus_tag="IW662_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0867093.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2965..3867
                     /locus_tag="IW662_002289"
     CDS             2965..3867
                     /locus_tag="IW662_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0867094.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3900..4250
                     /locus_tag="IW662_002290"
     CDS             3900..4250
                     /locus_tag="IW662_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0867095.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4284..5222
                     /locus_tag="IW662_002291"
     CDS             4284..5222
                     /locus_tag="IW662_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0867096.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5300..6193
                     /locus_tag="IW662_002292"
     CDS             5300..6193
                     /locus_tag="IW662_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0867097.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6231..6794
                     /gene="amaP"
                     /locus_tag="IW662_002293"
     CDS             6231..6794
                     /gene="amaP"
                     /locus_tag="IW662_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0867098.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6808..6996
                     /locus_tag="IW662_002294"
     CDS             6808..6996
                     /locus_tag="IW662_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0867099.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7009..7911
                     /locus_tag="IW662_002295"
     CDS             7009..7911
                     /locus_tag="IW662_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0867100.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7943..8185
                     /locus_tag="IW662_002296"
     CDS             7943..8185
                     /locus_tag="IW662_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0867101.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8441..8812)
                     /locus_tag="IW662_002297"
     CDS             complement(8441..8812)
                     /locus_tag="IW662_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867102.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3113 a         1549 c         1734 g         2546 t
ORIGIN      
        1 gcagacggct cataaccgtc cggtcgtagg ttcgagtcct acaaggtaca taagaggttg
       61 tgaaaaagct gccctgcatc aagtaataag aacagccaag taaaaacagc ttcttatgtt
      121 tttacttggc tgttcttatt actgcagatg caagcttttg aataccgttt aaggaagagg
      181 ttgtgaaatc agcgttttga cctgagtatt agcttaacaa acggaaaaat agttcctcat
      241 attttgccgt ttgttaagct aataccgagg gtcagctgat tgaacaccat ttaagaaaga
      301 ggtcgtgagc ctgccgtttc actctgtgtc ataaaaaagc caatctactt tgatagaaaa
      361 agcacgtagg actaaaacag ttctgcgtgc tttttttatt gtgtgttaaa tatcgtaaat
      421 gaagatagat atttttatga ttcgacgata gagacgttga aatctttcaa cagctctaaa
      481 gcggattctc cgtgttttct actggagact aacattggat caacaaaaaa gtctgcttca
      541 ggaaaagcac tctgcaggcc tatcagatta gagagtacac acaagtttgt ttcaatcccg
      601 actacttcga tctctttttg ttcttctttg ctttcaaata aagtttcctg gatttcagct
      661 aaagtttcag gtggaatcgt aaagtaatac tttttaatca ctcgttcatt ttcttgtgga
      721 gaaagaagag ggatcaattg caaatcttct cgttcttctt ctttgtcctt cagttcaact
      781 ggtatatccc gtgtgaagag aatatattca ttattttggc gtgctttagc taatctttga
      841 gcaatacgtt ctgccaattg atctgactct ggaatataaa attcgctgtt tggatcaaga
      901 atatgatttt gcatatcaat aataactaac attttctttc ctcctacatt catatcgtca
      961 ttttattctt tttaatagaa gttttttaaa atataagcaa caccagcttc ttcattcgtc
     1021 agtgttactt ggtttgcaat ttcttttact tgtcgctgtg cattccccat cgcaatactc
     1081 aacccgctgt aattcaacat atcaatgtcg ttttcttgat cgccaaaagc catgatgtca
     1141 ctcgattcga ttccaaggta atccgccaga aaaacaagtg aatgagcttt agtgatacct
     1201 gtaggcgtga tttctaagaa atgcggatca gagaatactg attggatacc atctgcttgc
     1261 agatctcttt gtagcttctg tagtttttct tcactcttat ctgtgaatgc taatttaaga
     1321 tagctaagag aaggatcttc caacatattt aaaatttgat caaaagaatg gttctgtaaa
     1381 ttgaaatgtt cataataagg gtcatctttg actagatttg attcatcgaa tgtcagctga
     1441 tcatcttggg tttctcggct agcaatgatg gatattttcg cttcttgagc atagaagaag
     1501 gcttttctca gtgtatcgct atctattttt gaacgtctga gaactttcgc catttttcca
     1561 tccttcattt cagcaatcag tgaaccattg gaagaaatca aatagccacc gattcctaat
     1621 tcggaaaaaa tatctttagc agaaatgctg ttccttccgg tagcgacggt tacgattccg
     1681 ccattttcat caaatttccg aagtgcttgg atattttctt ctggtatctt tccatttcga
     1741 tcaagcaagg taccgtctaa atcaatcgct gctagtttca ttttatcttc ctactttctt
     1801 tttacatatc tattttcctg ttaccttaag attaaatgag aatttaaaaa aatgaaacta
     1861 acttgctttg tcattatgaa ttttttcaaa aaacattctt aagaaaaagg tggctatata
     1921 tagtagtttt tggtcaatac ctttgaataa aaagccatga aaaacgaata gttagcgctt
     1981 accgatgcgt tttgtgataa aaaaatttta aaatattcaa tgaaaacaaa gcatatattt
     2041 tgctttaaaa ccaaatgagg aatattctag atgtagaaca ggttagaaaa agattagaaa
     2101 aaggagtgat tttgatggag aagagatacg gcgtagttcc tgtggtaact gcttcagacg
     2161 aaaattatgc tccttacttg agcgtgatga tcgcaactgc attagaaaat tgcaataaaa
     2221 caagacgcat aaaattttat gtgattgacg atggtctatc agaatatagt aaagaaggat
     2281 tggaagaaac ggtaaataaa tattcaagta atgcaagtat ccaattcctg acagtggaaa
     2341 aagatatcta cgaagacttc ctagtcagtg accatatcac aactaccgct tacttaagaa
     2401 tttctttacc aaacttatta gctaaagagg attataagaa agtcctttac ttagattcag
     2461 atgtgttagt actagacgat atcgtaaaac tatatgatga gccgttaaat ggaaaaacaa
     2521 tcggcgctat tatcgatcca ggccaagtga aagctttgga aagattagga atcgattccg
     2581 atgacttgta cttcaattca ggagtgatgg tgattgacat tgatcaatgg aataaaaaag
     2641 aaattacaga aaaaactatc cattacctca gtgaaaatgg cgatcgaatc atctatcacg
     2701 accaagatgc tttgaatgct gtactttatg aagattggga acaacttcat ccaaaatgga
     2761 acatgcagac ttcgctgatt tttgaacgtc atccagcacc aaatgaaaaa tatgaacgtc
     2821 agtataaaga aggaaatgaa aaaccttcga tcgttcactt tacaggacat gataaacctt
     2881 ggaatacgtt gaaagatcat ccgtatacca atttatactt aaaaaaatta gcacacagta
     2941 ctctaacgaa agtaggcgag atcaatgaat aaaaaagaaa tagcagtagt cgcaagttgt
     3001 aacacaaaat tcgtaccaca tttagctgca ttatttgtct cagtattaga taattgcaac
     3061 ccgtctaaat ttgttcggtt ctatgtgatt gacgatgata tcgattttga aagtaaacag
     3121 cttttacgct tctcagtgaa aaacgcacga atgaactcag atgtggaatt cttgaaaatc
     3181 aataaagaat tctttacaaa tgtggtaatc agcgatcgta ttccagaaac agcttattat
     3241 cgtattgcga ttccggaatt gttccgcggc acagaagttg aacgtatctt atatatggat
     3301 tgcgacatga tcgctttaca agatatctct aaattatggc gtcttgactt cggtgattca
     3361 atcgtagcag ctgttgaaga cgctgggttc catcaacgac tggaaaaaat gaaaatttca
     3421 gctaaatcaa tgcgttattt caactcaggt ttgatgctga tcaatgtgaa aaaatggtta
     3481 gatgaaaata ttacgcaaaa agtattagat ttcatcgaac ataatccaga aaaattacga
     3541 ttccatgacc aagatgcatt aaatgctatc ttacatgacc gttggttacc acttcatccg
     3601 cgttggaatg cacaaggcta tatcatggca aaagcaaaaa aacatccaac agcagcaggt
     3661 gaacgtgaat acgaagaaac tcgcaacaat ccttacatta tccatttttc aggacatgtg
     3721 aaaccgtgga gcaaggattt tgaagggcca acgaagaaat attacgaaaa atatgcaggg
     3781 atgacggctt tccgttgtgt agctaaattc cctaaatatc caaaatatgc aaaagttcaa
     3841 ccaaaaacag atgtagttgt taaataatag aagacaacaa ctgaagaggg ggagtaggaa
     3901 tggaaattac aatgtctaaa aatgcagtag agacgttaat tgaaaaggtt ggggaaaata
     3961 caaaaatcgc actcgcgctt atcaatgatt ccgatccatt tctaagggac aaaggtgctt
     4021 ttgcaaaagg cagctttttt caaatcattc cttttgtttc ggaatttgga gaatatgcga
     4081 ctaaaatcga gcatccatta ttggacatct atacatctaa attagagcaa aattatttcg
     4141 gtaaacgatt gaacatggac ttcaacaaac agcttgacag cttttcatta gaaaatgaaa
     4201 tcgcagtact cgattataat atcaaattaa agaactgctt cttcagctaa tagtttcgag
     4261 aaagtccaaa aaggaggtat acattgagat actactttgt ggatgaagaa aagaatgtaa
     4321 tagaaacaga tgaaaaaaaa tataattggc tcgtacttga tagtgcaaag cccgaagaga
     4381 ttgagcaagc aatcgaagaa ttcgagcttc ctgatgatat tttcgtagga accgaatatc
     4441 cggaagaagt ttctcggatg gagcgccttt atgagacgaa actagagcat ccattttctt
     4501 tggtagtgat taatctagat cagtcagata atcgtatcga aaaaaaatta acgccaatat
     4561 cttttgtact ttccgataat ctcttgatta cttgtatcga tagaaaaacc gaatttgtgg
     4621 atcggctgct gacaaagcat ggaaatcatt taaactcgtt tgaaaagatc atcacgtata
     4681 cattattaga tatttacacc cattacgtga aagaactaag agagatgaaa aagcgaattg
     4741 acgctttgga taaagcagct cgtaaaacaa cggaaaatga agaactgtat aaacaggcag
     4801 atcttgaaag agacatcgta tatatcgatc ataccttaag agatcaacgg gaaacaatgg
     4861 atcaattatg gaattctgaa actttcatca aacgtttaga cgatgaacag ctactctatg
     4921 atgtccattt gagacagcga caaacagaaa aaatgatcga gatctatcga gatttattag
     4981 agagtattgg tgatctattc aacgggatga tggacaataa tctaaatcat atcatgaaat
     5041 atctagattc agctgcatta atcatttccg ttccggcgtt gttcgcaggg atttggggaa
     5101 tgaatacagg aggattaccg ggtaaaagtt ctgctctagg ctttatactc gtagtctttg
     5161 gctctttgat agcagcagta gttttaggtt accatttata ccggaaagat tatacaaatt
     5221 aaagaatcta tttaaaacag gtatcaaaca aagaaatttg ttttggcaat acaatttaaa
     5281 aactaggagg aatacagcaa tgacagaacc aaaattaatc gacccgcgaa acttgtacca
     5341 tacggagaaa tttcctaaac aagatcagga tacaccagct ttacagcaga atatgattcc
     5401 tgttcctgat tgtggagaag aatcctatga aggcaataat caactagaaa accgccgtgt
     5461 actaatcact ggtggagatt cagggatcgg tcgtgcagca gcgattgcat ttgctagaga
     5521 aggggcagat atcgctcttc aattcttccc aggagaagaa aaagatgcag aggaagtagc
     5581 aaaatacgtg aaagttgcag gacgtaagat tgtcttacta ccttttgatc ttcgtgacga
     5641 acaagcacct aaagaaatca tagataaagc cgtgagtgaa cttggcgggc tagatacgat
     5701 ggtactaaac gcaggacagc aaatttcttg tcagtctatt gctgagttac cacttgagca
     5761 agtcaaagac acatttatga taaacatcat cgctatgttt gctttagtga aagaagctgt
     5821 tccgcatatc ccagcaggag gagcaatcgt aacaacaact tctgttcaag cattcaatcc
     5881 aagcgaacat ttattagatt acgcagcaac aaaagcttca atcgctaact ttacgatcgg
     5941 tttagcaaaa cagcttgcac ctaaaggaat ccgtgtaaat ggtgtagcac ctggtccaat
     6001 ctggacacca ttacaactag acgaaggaca accttctgat tcgatccctg aatttggtca
     6061 gcaaagttta ctagaacgtg caggtcagcc aggagaatta gcaccagttt atgttttctt
     6121 agcatcgaat aaagcaagtt atgttacagc ccaaatttac ggagtaactg gtggagaagc
     6181 gatcaactta taagaagttg atcgtttcac ccctatgagg aggtgcgaaa atgaataaag
     6241 gtgtaaaaac gatcggagtg atcggaacat tacttttgct atctgtgctt ttgtttgcga
     6301 tactattaag tgcaacctat ccattgcctt ttcagctaga gaagtttcga ttcttttcca
     6361 tcactaattc atacgtgcaa caatatgtat tttggcttgc cgttgcattt gctattttga
     6421 caattatcac gttacttgtt ttcctgtttt atccaaaaac aatcggaaac tttctactga
     6481 aacatggtga tggggagttg acgttggata aaaaagcaat tgaaggaatg gttcgttctc
     6541 acttgcatga agatgaattc atccattcac caaaagtaaa tgtcaaagca acgaaaaaca
     6601 agatcgccgt caatgtaaaa ggggagttga aacgaacttc agcactcatt ggtaaaacag
     6661 gaacattgat gaaagaaatc gaaaatgaag tacaacacat attgggaaca aaagagccag
     6721 tgaaagtatc ggtgaaatac acaggctttg aagaaccagc taaacgaagc aatgaccgca
     6781 aacgagtaga ataggggggg accaaaaatg aaagaactat tttcgacata caagctccct
     6841 atcatctttg gggtacttgg attgatatta gccgttttat ggatcaccgt gggctttttc
     6901 aagaccttat tgatcttgat catggcagcg atcggcgtcg ctatcggttt ttatctgaaa
     6961 caaacgaaaa tcttagatca ctattttaaa tcttaggagg aaacatccat gaataacaat
     7021 aacaaaaata ataacaataa aatgaacaac caaaaaccag tgtctgcaca tccagaccca
     7081 acgacaccaa accaatcaaa tggtgtgaac gatgaaaaga acaaaacagc accagtatct
     7141 gcacatccag atccagtagt ccctaacgtt caacaaggag tagaagagaa gaaacatgat
     7201 gcagaaaagc atgcacctca tcatccagga actccagaat ctaaagttcc agaacaacat
     7261 gatgcaatcg atccttctaa acctgtggaa gagcaaaaac atccttctga ccatgcgaaa
     7321 gcacaagaaa aacttagttc tgaccacaca aacggacaag gaaaacttgg ttctgatcat
     7381 acaaataacc aaggaaagca tgattctgaa caggaagatt caatgcgcgg tgagctgaat
     7441 tatgaagata aagttgtgca aaaaatcatc ggaatcgcaa ttgaacaagt agacggttta
     7501 ttatctgcaa acggtggttt cttctctaat gtggcaggaa aacttgtgaa tacagataat
     7561 gtaacggctg ggatcgaaac agaagtcggt aaaaaacaag tagcagttga tatggatgtc
     7621 attgtggaat atggcaaaga tatcgaaaaa atctttgaag aaatgcaaga agtaatcggc
     7681 aaagaagtaa aaaatatgac acatttagaa gtgatcgaag tcaacgctaa cgtagtggac
     7741 atcaaaacaa aagaagaatt tgaaaaagat caagaaacgg tgcaagacaa agtaactgac
     7801 gcagcgaaaa aaaccggtga atttgcttcg aatcaaacag ataaagtaaa atcagcagct
     7861 ggaagcggcg cacaaaaagt aaaagaaaat acagaaccac gggttcagta ataaggaagt
     7921 aaaacattag gaggaacgaa acatgggatt tatttggtca cttatcgtcg gaggaattat
     7981 cggagcaatt gcaggagcaa tcacgaataa aggttcttcg atggggatta ttgcaaatgt
     8041 cgtagcaggt ttagttggat cagcacttgg tcaagcatta ttaggttctt ggggaccaag
     8101 tattgcagga atggcaatcg tgccatcatt gattggtgcc atcattttag tactaatcgt
     8161 atcattcttt gctcgaaaac tgtaaaaaga cttttaaaaa gaggtcgtga aaaagctgtt
     8221 cttcatcaag taataagaac agccaaacaa aaatagcttc tcatattttt catttggttg
     8281 ggcatattat cgaagatgca agcttttgaa cactgtttat acggaataag aggctgtgac
     8341 aagacttttg tcacagcctc ttatttatgc gggcaaattc ccaattagat ttttaaaagt
     8401 ttttccttaa atgtgtcttt attgatacag agaagcttct ttaataaaac aatgcctccc
     8461 taaattttct tgagatagga aaggtgaaca tacaaaataa tacgaccact ttgaaaaccc
     8521 agatgatcca caaatggatc gtatgatact gatgaagtaa aaagcaatgg atcagaaact
     8581 caaagagatt gaaaaagaca aggaagaaat aatatctctt ttttactctt gtctgtttta
     8641 gccaataata gctcgtaaag taatctagac aaacaaatag aagtaaacaa agtttcgtaa
     8701 gagcagcaat cgtatcatgt tgctctgcta gacgattgac gagcaggaaa aatgttggta
     8761 atggaatata gatcacgagt aatgaaagaa aaaagtaaac aataaatggc atactattcc
     8821 tcctaattgt gtgagttcgc cctcatttta aaataacgat atttgtttgt gaagcttttt
     8881 cgtgtcgtaa caaatgttat ctaatgtatt tttaaataaa taatcattat tatcaaaata
     8941 at
//
LOCUS       DACEKH010000100         8661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEKH010000100 DACEKH010000000
VERSION     DACEKH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.104"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="IW662_002298"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="IW662_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ0867103.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="IW662_002299"
     CDS             complement(738..1460)
                     /locus_tag="IW662_002299"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ0867104.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="IW662_002300"
     CDS             complement(1596..2477)
                     /locus_tag="IW662_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ0867105.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IW662_002301"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IW662_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ0867106.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="IW662_002302"
     CDS             3717..4196
                     /locus_tag="IW662_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ0867107.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="IW662_002303"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="IW662_002303"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ0867108.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="IW662_002304"
     CDS             5497..5625
                     /locus_tag="IW662_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0867109.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5629..6174
                     /locus_tag="IW662_002305"
     CDS             5629..6174
                     /locus_tag="IW662_002305"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0867110.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="IW662_002306"
     CDS             complement(6628..8319)
                     /locus_tag="IW662_002306"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ0867111.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
BASE COUNT         2410 a         1771 c         1568 g         2912 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaaccca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta ctgtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8341 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8401 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8461 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8521 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8581 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8641 tagtcgtttc ataaaaaaaa a
//
LOCUS       DACEKH010000101         8555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEKH010000101 DACEKH010000000
VERSION     DACEKH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.105"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="IW662_002307"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="IW662_002307"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0867112.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="IW662_002308"
     CDS             complement(1098..2201)
                     /locus_tag="IW662_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0867113.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /locus_tag="IW662_002309"
     CDS             complement(2291..3226)
                     /locus_tag="IW662_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ0867114.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="IW662_002310"
     CDS             3528..5462
                     /locus_tag="IW662_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0867115.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTENSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="IW662_002311"
     CDS             5919..6335
                     /locus_tag="IW662_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0867116.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="IW662_002312"
     CDS             6349..6915
                     /locus_tag="IW662_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ0867117.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="IW662_002313"
     CDS             6938..7924
                     /locus_tag="IW662_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0867118.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8555)
                     /gene="scm"
                     /locus_tag="IW662_002314"
     CDS             complement(8079..>8555)
                     /gene="scm"
                     /locus_tag="IW662_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0867119.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2517 a         1651 c         1736 g         2651 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactga aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc catcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgct
//
LOCUS       DACEKH010000102         8482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEKH010000102 DACEKH010000000
VERSION     DACEKH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.106"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            671..955
                     /locus_tag="IW662_002315"
     CDS             671..955
                     /locus_tag="IW662_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0867120.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            958..1875
                     /locus_tag="IW662_002316"
     CDS             958..1875
                     /locus_tag="IW662_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0867121.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            1868..2650
                     /locus_tag="IW662_002317"
     CDS             1868..2650
                     /locus_tag="IW662_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0867122.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            2651..3331
                     /locus_tag="IW662_002318"
     CDS             2651..3331
                     /locus_tag="IW662_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0867123.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            3324..4220
                     /locus_tag="IW662_002319"
     CDS             3324..4220
                     /locus_tag="IW662_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0867124.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            4415..6064
                     /locus_tag="IW662_002320"
     CDS             4415..6064
                     /locus_tag="IW662_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0867125.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVCSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            6658..7593
                     /locus_tag="IW662_002321"
     CDS             6658..7593
                     /locus_tag="IW662_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0867126.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            7586..8356
                     /locus_tag="IW662_002322"
     CDS             7586..8356
                     /locus_tag="IW662_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0867127.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         2646 a         1457 c         1847 g         2532 t
ORIGIN      
        1 agcctaaaaa tttaatctaa aatacgaaat atactgcctt taaattagaa cacattttag
       61 aacacaaaca tttcaaattt aatcaaatat aataaaatat atttacggga aaacctttat
      121 aaatcaaggt ttttcaaact tattcaatga tacgaagaaa aaaccaggtc ttaagaaagc
      181 ccgtaaagct tcacagttct caaaacgtta attgcgatta tcgttcgagt tcacgagtta
      241 cgagaatcgt actgggcgaa gcaatgctga gcgcagttac cttaatccta acttttctgg
      301 cactttccac aatttggaaa gtgccttttt tgtgttttta gctatgttaa taggtaggat
      361 tcttggtcta taatatcatt cgatctataa acaaacggga aaaagataaa gtatcatcaa
      421 acaatattat ttatccgctt ctgaggttgt gacagaagtg gtcagcttca agaaataaga
      481 aggaatttac aaaaattgct tctcatgttt gataaatatt gtgacttgtg accgaggagt
      541 tggcaggaga acaccgttta ttcggaagaa gggattgtga aaagtctttt gtcataacct
      601 cttatttggt agctaattgt aaatcccaaa cggcatgtat tatttttcat aagtccgttt
      661 ttaaggatta atgttttctt ttgcttttta tatggtctgg cttcagcgag aagttccaac
      721 gcatatacaa ggacgtctga tgtcaacgat aacaacgatt accatggtgg ccagtccact
      781 tggattagca ctgtttggtg cgctctttga ttggcagaca actcaaccac gtctgcgaga
      841 cagtctgatt tttcttgaaa ttcaattgga aaatttacac tttctttata catttctggc
      901 tgctttcttt atacccgtca actatacttc acttatcaat aagaaagaag gataagtatg
      961 gattttggaa atgaaacggt cttgaaagca actggtataa ccaaaaaata cggtgctgcc
     1021 aaagcattag ataaagtatc gatcgaaatc aaacgtggaa tgatctatgg tttgattggg
     1081 gagaatggag cgggtaagtc tacctttatg agaactatta tgggtttgat ctcgattgat
     1141 gaaggttcga tcgaattatt tggaacgacg gatttgcagg cagcaagacg acgtatgggt
     1201 caatcgattg aaacccctgc tctttatcca gaattaactg caagagataa cctacgtatc
     1261 caagcagcga acggcggtgt gtcggataga gaaatcgaag acctgctgaa aatgatgcga
     1321 ttggaaaata cagggaaaaa gaaagcaaag aatttctctt taggtatgcg ccagcgttta
     1381 gctattgcaa atgcactgat cacgaatcca gaatttttga ttttggatga accgacgaat
     1441 gggatggatc cagcagggat ggcagaaatg cgagaaatca tccaacgttt ggtaaaagaa
     1501 cggggcatca ccgtcttgct ttctagccac ttgctagatg aattgtcaca gatcgccact
     1561 cactacggta ttttgcatga aggacacttg atcaaagaat tatcgaaaga agaattggca
     1621 caagaatctc gtcaatttat aaaaattgac acttcggcga cggaacaagc agtaacggtt
     1681 ctcgactctc ttggttatag ggattatttt gtccaatcta gccgtgttat ccagcttttt
     1741 gaaggaattg accaagtagc agccatcaat caagcattgg tagaggcaaa agttcctgtg
     1801 gatggtatcc acctagttgg gcaaaaactg gaagattatt tcttacaatt aactggaggc
     1861 aatccacatg tttaatctat taactgctga aaaaataaaa gtatggaaga gccgtaaaat
     1921 gtgggtctca ctaggactga tggtcgtact tccgcttttt tattcttgga acgaatggta
     1981 tatgcataca aaatacggca atgaattaaa ccaagcaacg gatacagtca tcaacggtgc
     2041 aacagggatt ttaatggtgg aaaaaatggc tgcatggatt cttctggctt tcgcagcatt
     2101 tgcgtgcttt tatattgggg aagaatttca aaacggtacg attcgcaata cgttatcttt
     2161 aggacggaat cgaatgactt attatgtgtc gaaacttctc gttacgcttt tgatcacaac
     2221 acttggcgtg gttttgatta cggggctagc catgattgct tacacacttg ctttcggatt
     2281 tggtgaagtt gagggaatca aaaactatgg aaattatgtt ctaaaagtat ttcctgtatt
     2341 gcttctgctg atattagcga cattatctgt tccagtggct ttgacattca tcactagaag
     2401 taccagtgtc agtttgcttc tttcttttct ttatatcatg ggaacggcct tcgttcccgg
     2461 tgttttcgca aaaatcaaag gcttggaatt tctaacggag tggtttacgg aaacgtggct
     2521 catgtatacg gactttgcgc aacaagcaac ttattcgcaa gcaccgaaaa tgattctggt
     2581 cagtctagta acaatcgtgg tatcatcagc tctaggaatg tttattttcc aaaaatctga
     2641 cattaagtag gtgttcctgc tggaaactat tttgattatc gaagacgatg aagctgttca
     2701 ttctttactg gaggaggttc ttgaacagag atacaaactt cttgatgctt attcaggtac
     2761 ggaaggaaaa ttattgttaa gcacatatcc tgtcgatttg atccttctag atctcatgct
     2821 tccagggctt tcaggtgaag cattgttagc agaaatccga cagacatcga atgttccgat
     2881 catcgttctt tcagcaaaaa gcgatcaaag agataaagtt tccttgttgg cagcaggagc
     2941 ggatgactat gtgacgaaac catttgatat cgaagaactg ttgttacgaa tcggcatcca
     3001 acttcgtcat cagacttctg tccaagtaaa agatctgtcg caacgcatct attacaagga
     3061 aatcttactg gacaaagagt cgagggatgt aaatgtgaat ggtgtggctg tccatttgac
     3121 aggaagagaa ttcgatctgc tgaagctttt tttagaacat ccgaaaaaag tattttcgcg
     3181 agcaaatctt tatgaaacag tttggcaaga accatatttt gatagtgaga aaacagtgaa
     3241 tatgcatatt tccaatcttc gagggaaact agcaaaggca catgctgtgt atattcatac
     3301 ggtgtgggga gtggggttta gatttgatta agtggctgtt gcttctcgtt gttatcttta
     3361 tgggaagttt tattttcttc ctgcaaaggg aaatcaagag attaactaag aaaatcgcca
     3421 ctctttctga acgacaagaa tatggtggtc gtctttcagt cgaatttcga gaaaagaatc
     3481 taatggacct agtagatgca ctgaatacat gggtggatga aaatgaacaa aaaggacaag
     3541 catttcaaga aatggaagaa aacatcagac tatcgatcgt cggcctttct catgatctca
     3601 gaactccttt gactgctatt aacggttatg tacaattact ggaacaggta gaggatacgg
     3661 agaaacggga gaagtattta acgatcatca aacaatctgt tgcaagatta ctcagtatga
     3721 cggatcagtt ttatgatctt gctcgaatag agacgaagca aaaagaaatg gatctccata
     3781 cgctttcttt ttctaaagag gtggaagaag tatttttctc tttttatgaa caattttctg
     3841 agaaaaatat cgaggtagcg ttcgctgaac actcgataaa tacaactgta ttagcagatc
     3901 cgctcatgtt gctgcgagtg ctgcaaaata tcatacagaa tttattgcgt tatgcagatg
     3961 gacaagcaaa ggtgacattt tcaaaagagg attcattcat tcgattaaca gtggccaatc
     4021 ctatcaaacc aggagcgcaa acctctgttg aaaaagtctt ccagcgtttt tataccgaaa
     4081 ataccagccg cacaaatacg gaatctagcg gaatcgggct ttacctgtca aaaaaattgg
     4141 tggaaggaat gggaggagag atgaccgcta gattagacgg gggcatcttc tccatctccg
     4201 tcaagttgcg gagggtttaa ttggctctaa tttacctgaa gtgaatgaca aaaaattgta
     4261 tctcgtttta aagacatctt cttttttgga aggtgttttt attttggtgt gattgatacg
     4321 aattaggtaa cgaagacagt tatttttgta cccccttttc aaatagcgca acagtgctat
     4381 actaaaacta acaaatcaag gaacggagag ggatatgggc caaagagaat ttatcgtttt
     4441 aattattatt gcagttgtta ttttattagt tgttttagac attttttcac gtttgaaagt
     4501 aaaggaaaca gtttgtagta attggggaaa aattccttat caaccgagat ttgataaaga
     4561 agaaagtttg aaggatgcgt ggctcacgga gaaaaaattc cgttcatggg atagtgaaat
     4621 cgacgatctt acttggtatg acttggatat gttcgaagta tttgaaggga tcaacagtac
     4681 ctattctagt gtaggttcag aagcgttgta tcagcgttta cgtagctttg attttggaga
     4741 agattatcag ttagaaaaac taattgcttt ttatcaggaa aatcctcaac tgagagaaag
     4801 aatccaatat caattcgctc gtttaggaaa aaaagatcat aattttgcaa aacaatatct
     4861 agcggatgga aaaagtaaaa agattggacg cgtaggttta tttatttttc ttggcttgct
     4921 tccgctggtg ggtgtaggtt tgcttctttt agggcaagta ctaggaatct atctagcaat
     4981 cggtggctta gtattcaata cggtctatta tctagtgaag aaacaaacac tagaaacaga
     5041 gctgaatagc atgagttatt tggtttcaac gatttcagcc gccaatcagt tagcaaaaat
     5101 gacgacacca cttcaaaaag agctgaaaca gcaaactaaa ccattaagag acatccctaa
     5161 attaggtttt tcatttcgaa tgaagagcgg gagtgaagca gaattgattt ttgaatatgt
     5221 caacatcatg ttcatgctgc catttatctc ctatcatttc gttctcacta aattagaaaa
     5281 gcattcaact gaagccatca agctgtggga attgctggga gaattggaag ttgcagcagc
     5341 cgttttgaat tatcgcacct atatgccgat cacttgccga ccagagttta ctgagggcgg
     5401 tgtgacagcg aaagatatct atcatccatt gttaaaagaa gcggtagtta atccagtcaa
     5461 ctggcagaag aatacattag tcacgggttc caatgcatca ggaaaatcta cgtatgtcaa
     5521 aagtatagcc atcagctgta ttctagcaca gacgatccaa acagcgattg cagaacagtt
     5581 taccctgcaa cctggacatg tgttgacttc catggcaatc gaagatgatt tatttgaagg
     5641 cgacagttat tttgtttcag aaatcaaatc gatcaaacgt ttgctggacc aagtggcttc
     5701 caaagaacgc tgctactgct tcgtagatga gattttaaaa ggaacaaata cgatcgaacg
     5761 gattgcttct tccgcttcag tcgttcggtg gcttgcggag tatccatctt tagcatttgt
     5821 cgccacacat gatatcgaat tgacagagat tttaaagaat tattgtgaga atgtccactt
     5881 tgaagaacaa gtaacagaag gaaaaggaat cagttttgat ttcaaattaa aagaaggacc
     5941 ttcaaggacc cgaaatgcga ttgccttgct gaaagttttg aattatccta aatccatagt
     6001 tgaacaggcc caaaaagaaa gtttgttgtt cgatgaacag cgtcagtggt atccgtttga
     6061 ttaactttat caaacgaagc atgatgcgtg ggtccaaaaa gacgattatc aaaagaagta
     6121 cattgatgcc aaaaaagtgg cagaattcaa tgggaacgag tctgctgtcc agatgatctt
     6181 ggaagattta gcaaaagacg atacaaaata aaccacgctg tcttccttct agcagctttt
     6241 atcgaaaaaa cttagcatca gcgacgagac gcacgttcca tggcaaagtt ctccgtaaaa
     6301 caaacagcga aaacagaaat tgaaaataac tatttccatt ttatttattt cggttaaaat
     6361 aaaagaaaaa agctttcgat aaaaggacgg aggaaagctt aacatgaata aaaaagacaa
     6421 gaacctcagt tataaggatc ttctatatgt catcgtagta gttgctgtcg gtacttttat
     6481 gattttaaaa gttccagagt tgtttagata gttattctat cgaagatgat catgaactag
     6541 aaaacacatc tcctctttgt tagcaggtgt gtttttgtgt ggaagccaag ttgttttttc
     6601 atctgctgta ttccatgata caattaagaa tcatgtctat aaggagatag gaaataaatg
     6661 gcaaatgcgc ttgaaataaa taatttgaaa aagatctatg gaagtggggt cgaagcattg
     6721 cggggaatcg atctttcggt gaaagaagga gatttctatg cgctattagg gccaaatgga
     6781 gctgggaagt ctacaacgat cgggattatt acttctttag tcaataaaac atctggaaaa
     6841 gtgaatgtct tcggttatga tctggacaag gatctagttc gagcaaagca acagattgga
     6901 ttggttccac aagagttcaa ttttaaccct tttgaaacgg tccagcaaat cgtcgtgaat
     6961 caagcaggct actacggtgt ctctcgcaaa gaagcgatga aacgcagcga aaaatatttg
     7021 aagcagtcca atctttggga aaagagaaat gtccgtgcac gtatgctttc cgggggaatg
     7081 aagcgaagac tgatgattgc tagggcattg atgcatgaac cacgtttgct gatcttggat
     7141 gaaccaacag caggagttga catcgagttg agacgcgaga tgtgggagtt tctacgagaa
     7201 ttgaacgaaa atggtacaac gatcattttg acgacgcatt atctagaaga agctgaaatg
     7261 ctttgtcgca acatcggaat catccaatcg ggtgaactga tcgagaatac aagtatgaaa
     7321 gagcttctgt caaaactgca atttgaaaca tttatctttg atttagcgcc atatgatacc
     7381 aaacctgtga ttgaaggata tgcttgtaca tttgaagatg agcagacaat cgctgtcgaa
     7441 gttgaacgaa atcaaggggt gaacggaatc ttcgatcagc ttacaagaca aggaatcaaa
     7501 gttctttcta tgaggaataa gtcgaatcgt ttagaagaac tgttcttgaa aatcacagaa
     7561 gaaaataatc atacggagga gaaaaatgtt taatctttat tttactgcat taaaaagttt
     7621 agctatcaag gaaacgaacc gctatttgcg tatatgggtc caaacactgg ttccgccagt
     7681 gatcacgaca tccttgtatt ttgttatttt cggaaaaatg atcggtgggc ggatcggtga
     7741 catgggtggt ttttcatata tggaattcat cgttcctggt ttgattatga tgtctgctat
     7801 cactagttcg tatgccaatg tttcttcgtc cttcttctct caaaaatttc agaaaaatat
     7861 cgaagaaatc ttggtagcac ctgttcctac gcatgtgatt atttggggct ttgtgattgg
     7921 tggtgtgggt cgcagtattc tcgtcggctc tctcgtaacg atcatttcct tgttctttgt
     7981 accacttcat gtgtattcat ggttcatggt catcattaca ttattgatga cggcaatctt
     8041 gttttcatta gcaggactgc tcaacgggat ttttgctcag tcttatgatg atgtatcgat
     8101 cgtgcctaca tttgtcttgc agccgttaac ttatctaggt ggcgttttct atgccatctc
     8161 tatgttaccg cccttttggc aagctgtttc aaaagtcaat ccgatcgttt atatgatctc
     8221 cggtttccgt tacggattcc ttggaacgat cgatgtgcca gtagcaacat cgatgatcgt
     8281 acttgtgtta ttcatcgttg ttttgtacgc cgttacttgg tatttgatca ataaaggaag
     8341 aggattgaga agttagaaaa gaggttgtga aatcagcgtt ttgacctgag tattagagaa
     8401 acaaacggga aaatagttcc tcatattttg ttgttttttg agcgaatacc gagggtcagc
     8461 tgattgaaca ccgtttagtg ga
//
LOCUS       DACEKH010000103         8478 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEKH010000103 DACEKH010000000
VERSION     DACEKH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.107"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            163..1104
                     /locus_tag="IW662_002323"
     CDS             163..1104
                     /locus_tag="IW662_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ0867128.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1173..1904
                     /locus_tag="IW662_002324"
     CDS             1173..1904
                     /locus_tag="IW662_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ0867129.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2048..4228
                     /locus_tag="IW662_002325"
     CDS             2048..4228
                     /locus_tag="IW662_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ0867130.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4248..4730
                     /locus_tag="IW662_002326"
     CDS             4248..4730
                     /locus_tag="IW662_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0867131.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4746..5507
                     /locus_tag="IW662_002327"
     CDS             4746..5507
                     /locus_tag="IW662_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0867132.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5638..6972
                     /locus_tag="IW662_002328"
     CDS             5638..6972
                     /locus_tag="IW662_002328"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ0867133.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7279..7764
                     /locus_tag="IW662_002329"
     CDS             7279..7764
                     /locus_tag="IW662_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HAQ0867134.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIKSEGELN
                     E"
     gene            7757..8440
                     /locus_tag="IW662_002330"
     CDS             7757..8440
                     /locus_tag="IW662_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ0867135.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
BASE COUNT         2801 a         1485 c         1858 g         2334 t
ORIGIN      
        1 tttttctgtg cgaaacagga aagcgaagtc tttttaattc aaataataat attatgtaaa
       61 caaaaaactt tccatgcgta attatacaca agcaaataaa tcgtcaaatg cgaacgcctt
      121 ctttatactg tttaggaacg aaaagaagga aggaagaaat acatggatta tcaagtactg
      181 ctttattaca attatacaaa aatcgaagaa ccagaggaat ttgccaaaaa tcatttagcc
      241 ttttgcaaat ctttggatct taaaggtcgt attctagtag ctgaagaagg aataaatggt
      301 actgtgtctg gaaccgttga aaacacgact gcctatatga aagcaatgaa agctgatcct
      361 cgatttgctg atacgatgtt caagatcgat ccaagcgaag gacatgcctt taagaagatg
      421 tttgtaagat accgtccaga gcttgtaagt ttaaagctag aagaggacat tgatccaaac
      481 caagtgacag gtgcctatct ttcaccgcaa gaatttaaag aagctatttt agatgaacag
      541 acagtggtga ttgatgcacg gaatgattat gaatatgact tgggacattt tcgaggggct
      601 gtacgcccag atatacgcag ttttcgagag cttccagact ggatccgcga gcacaaagaa
      661 gaatttatgg aaaaacgggt agtgacctac tgtactggtg gcattcgttg cgagaagttt
      721 tctggttggc ttgtccgtga aggattcaaa gatgtcggac agctgcatgg cggtattgct
      781 acttatggta aggatcctga agtccaagga gaattatggg acggcaagat gtatgtgttt
      841 gatgaacgga tcgctgtcga tatcaaccaa gtagataaac aaatcgtcgg tcgcgactgg
      901 tttgacggaa caccatgtga acgatatatc aattgtagca atcctgagtg caatcgtcag
      961 attcttgctt ccattgaaaa tgaagaaaaa catttaggag catgtagcat agagtgtgcg
     1021 aaacatccgc gcaatcgcta tgttcaagac catggaatct ctccagaaga agcagaactg
     1081 cggtttacag ctttaattaa atagaaaaca atagttttga ttgtcgacca tttccaatat
     1141 gcgctatact agcgttgagg atggtgatcc acatgaaatc attgatttat gctattgggg
     1201 atgtccatgg caaacatgag ctttttgaaa aacttattca ctattatgat ccaaagatcc
     1261 atcagcttgt tttattaggg gatctcaatg atcgaggtcc tcgtacaaaa gaatgttttt
     1321 tattaggaaa gcaattagtc gaagaaacag gagccattta cctccgggga aaccatgaag
     1381 aatatttttt gcaatttctg caatcaccgg aggactggta tgcgccttat atgagaaatg
     1441 gtggaaaaga aacgtttgaa agcctgattc acccaggggc atcggaggaa tactccccaa
     1501 ctgaaatctc gatgatgatc caatcgagat attcagaact gattaaattt ttagtccagc
     1561 gccccttgta ttatgaatgg gaaaattatc tttttgtcca tgccggtgtg gatttgacaa
     1621 agaaagactg gcgtaagaca agtctaagag actttatttg gataagggaa ccatttcatg
     1681 aagggaaaaa caatacaggt aagacaattg tatttggaca tacgattaca ccaatgctcc
     1741 atggagatat gcagacaaca gacctctgga ttcaggatca taaaatcgga atggacggtg
     1801 gggctgtatt cggagggtca ttgcatgggg ttgtttttga caaggaaggt attcttcaag
     1861 atattgaata tcagaacaac gaaggacctt ggcatggaat ctgactaatg gaaagaatga
     1921 aggagctgtg actttgttgc ggctcttttt atttttatag agaatattgt ttctttcagt
     1981 agtttaaccc aggataaaaa gtatgataga ataaagaagc aatttttagc agaaaagagg
     2041 cattttaatg acggaaaatt tgaaccagac gatcattcag cttgtacaaa aagaactttc
     2101 agactatcgt cctaagcagt tgactactgt acttaatttg ttaaatgaag gaaacactgt
     2161 tccttttatc gcgcgttacc gaaaagaaat gactggaagt cttgatgaag tccagattcg
     2221 tgaaatcgaa gagcggtatg cctatcttga aaacttagaa aaacgtaaaa ctgaagtgat
     2281 tcggctaatc gatgaacaag ggaaactgac tcccgagtta gaagcagaaa tcacccaagc
     2341 cgtgaaaatg caacaagtgg aagatttgta tcgtccatac aaacaaaaac gacggacaaa
     2401 agcaacgatt gctaaagaaa aaggacttga accactcgcg aagtggctga tgcagcttac
     2461 agatggagag gtgcaatctg aagcagaaaa atatattgac aaagaaaaag aagtctcttc
     2521 tgccgaagaa gcgttgcacg gtgctcatga gattattgct gaacaagtca gcgataatgc
     2581 aaaatttcgg acttggattc ggtcttatac gtataacaaa ggtatgtatg tcagcaatgt
     2641 caaggatgag caagcagatg aaaaaggcgt atatgaaatg tattatgact ttgcagaacc
     2701 cgtccataaa atggtttctc atcgaatatt agctactaat cgtggagaaa aagaagatat
     2761 tctgaaagta ttctttcaag tagatgaagc agcaatttta gcttacttgg atcgccaaat
     2821 agtcaaaaat cctgcctctc cttcttcttc attcgtcaga gaagcctatc aggatagtta
     2881 taaacgcttt attcagccag caatcgagcg agaactgcgc aatgaattga ctgaaaaggc
     2941 agatgagcaa gcaatcgcta ttttcggcga aaacctccgt aatcttttgt tgcaaccgcc
     3001 actaaaaggt aaagttgtcc tgggatttga tccggcttat cgtactggat gtaaactagc
     3061 agtcgtagat gcgacaggga aagttttagc aatcgaagtt atttatcctc ataaacctgc
     3121 agcgcaagca aaaagagaag ctgctggacc agcttttatc caattaatta ataaatatca
     3181 agtagatatg gtagctatcg gaaatggtac agccagtcgg gaatctgaac tttttgtggc
     3241 tgagcagttg aaatccgccg accataaaac ctattacgct atcgtcaacg aagcaggcgc
     3301 ttctgtttac tccgctagcg aaattgcacg aaaagaattt ccgcatctac aagttgaaga
     3361 aagaagtgct gtttctattg cccgtcgcct gcaagatcct ttagctgaat tagtcaaaat
     3421 cgatcctaaa gctgtcggag taggccagta ccaacatgac gtttctcaaa aacgtttggc
     3481 tgaacaattg gattttgttg tggaaacagc ggttaaccaa gtaggtgtgg atgtgaatac
     3541 agcaagcccg cagctcttgc agcatatatc aggattgaat aaaacaactg cccaaaacat
     3601 tgtcatttat cgtgaagaaa acggagaatt caccgcacga actcaattga aaaaagtgcc
     3661 acgcttagga ccaaaagcat atgaacaagc aatcggattc ttgcgagtcc ctggtgggaa
     3721 aaacattttg gacaacacag gtatccatcc cgaaagttat tctattgcaa aagaaatatt
     3781 aacttcagtc caactttcag aaaaagagct tggtacagaa gaagcagtag aaaaattgac
     3841 acgattatcc gtagagaaac tggctgaaag cctatcagtc ggagaagaaa cgttagccga
     3901 tattttagct gggctgacac aacctggacg agatatgcgt gatgagatgc cagcgccctt
     3961 attacgtaca gatgtattga gtatggaaga cttgaaacca gggatggaat tgactggaac
     4021 ggtccgtaat gtcattgact tcggcgcatt tgttgatatc ggtgtgaagc aagacggact
     4081 tgtccatata tcgaaactca gcaaaaaatt cgtcaagcat cctacagatg tcgtgtcagt
     4141 aggagatatc gtaacagttt ggatagaaca agtcgatacg aaaaaaggca gaatcagttt
     4201 gacgatgctt tccccttatg aagagtaaca atgaggagta acaaaaattg acacaagaag
     4261 aactgcagca gttagtagaa gagatatcat taaaaagctt ccagcttcct ttcaagcacc
     4321 aagcaatgtt caatccacgc ttacgtacaa ctggcgggcg ctatcattta aacgatcatc
     4381 atttagattt taatgcgaag ctatttgaag agtcagacga agagacgatt gcaggaatca
     4441 tcaaacatga actttgccat tatcatcttc atctagctgg aagagggtat cagcataaag
     4501 atatggattt caagtatctt ttgaaaaaaa caggagggtt gcgctatgca ccagccttga
     4561 cgaagaaaca gataaagatg gagtattatc gatgccaaga ttgttctgcc acgatctgcc
     4621 gcaagagaag aatcgatgta cgcaaatatc gttgtggaag atgtcatgga gaaatcaaat
     4681 ggattgagac aaaaagagaa gactttaatg aagcacaaaa aatcagatga aaggatagaa
     4741 ggcctttgga aaaaagtgag cagaggaatc taaaagactg gaatcagcta ttaaattttt
     4801 tagccgcacc tgaagaggga aattttgagg aactgacggc acctacactt attttagctg
     4861 gaaactgtct tcctatctta gctgataaag cagcacaaat gtatttaaat ggccaagtag
     4921 atcaattgtt tttggtcgga ggggtggggc atgcgactag aatcctttat gagaattttg
     4981 aaaagcaagg atttcatttt gaagatggca tgagtgaaag tgaaatctgt cgtcagtatc
     5041 taaaagaagt ctatgatttg cccgataagg cttttctgat agaatcgaag tctacaaatt
     5101 ctggagagaa cgccactttt tcactggaga ttcttcattc cttaggagct gtaccagaaa
     5161 aaatcttatt gatgaatgat ccgacactgc aacgcaggac tcgagcaact tttgagaaag
     5221 tatggcaaaa tgaacaaact attttcgcga atgctgtgcc atttgtgccg gaaatccttc
     5281 acttttctga agagatcatc tttacagcaa aagaactgaa tcatcaatgg cctaaggaat
     5341 atttccatgc gctagtatta ggagaaatgg aacgtctcca tgacgatgag aacggctatg
     5401 gtccgaaagg aaaagatttt attcctcaca ttgatatatc agaggaagtt tggtcttctt
     5461 acaccagaat taaagagtca ataaaaacgg atttttctcg tacttgacca ttttagcaag
     5521 cgaatcctcc tttccaaata aatttggttg aaattttcaa tcgtttataa gataatgagg
     5581 agcgtgttga atgtaatagc taaaagaatg gacgctagat aagagaagag gattcaaatg
     5641 gaaaatcaaa ataaattgta tcattttgtt ggtatcaaag gttcaggaat gagctccttg
     5701 gctcttgttt tacatgaaca aggtctaaat gtccaaggat cagacattga aaaatatttc
     5761 tttacacaaa gagatttaga aaaagcaaat atcacgattc tgccatttaa cgcagataat
     5821 gtaaaaccag ggatgacgat cattgcagga aatgcatttc ctgattcaca tgaagaaatc
     5881 cagcgagcaa aagaattagg tttggaagtg attcgctatc acgactttat tggtcatttt
     5941 atccaaaatt atacgagtat cgctgtaaca ggctctcacg gaaaaacaag tacgactgga
     6001 ctgctctctc atgtattgag tggtgtccgt ccaacaagtt atctaattgg agatggaaca
     6061 ggacatggtg atccgcaagc ggaattcttt tcatttgagg cctgtgaata tcgccgtcat
     6121 ttccttgcct attcaccaga ctatgcaatc atgaccaata tcgattttga tcatccggat
     6181 tattatacaa gtatcgacga tgtatttaca gctttccaaa caatggctgg acaagtgaaa
     6241 aaagctattt ttgcgtatgg tgacgatgct tatttacgca aattaaaagc aaatgtgccg
     6301 atttattatt atggtgtcac agaaaacgat gatatccaag cacgaaacat cgaacgcaca
     6361 acttctggtt ctgcttttga tgtctatcat ggggatgaat ttgttgggca cttcactgta
     6421 ccagcattcg gtaaacacaa tatcttaaat gctttaggtg ttattgcagt agcttatttc
     6481 gaaaaactgg atctgaaaga agttgcagaa gaaatgctga ctttcccagg agtaaaacgt
     6541 cgattcagtg aaaagatcgt agcagacatg accgtagtcg atgattatgc gcatcaccct
     6601 gcagagatca aagcaacgat tgatggcgca cgccaaaaat atccagataa agaaataatt
     6661 gcagttttcc aaccccatac gtttacgcgt acgattgcat tgatggatga atttgcagaa
     6721 gcacttgatt tagctgataa agtttactta tgcgatattt ttggttcggc tcgggaagaa
     6781 caaggaaacg ttaagattga agaccttgga gcgaaaatca aaaaaggtgg agaagtcatc
     6841 aaagaaaata atgtatctcc attgttagat tatcatgatg cagtagtaat cttcatgggt
     6901 gctggagatg ttcaaaaatt tgaacaagcg tatgaaaaac ttttaagcag cacgacgaaa
     6961 aatgtactgt aatacaatta ctgcagtgtc cttataaaaa tagaaagagg gggagtggca
     7021 aaaatcttgc caagccccct tttttgcaac ttatgataaa cggtgttcaa aagcttgcat
     7081 ctgcggtaat aagcccaatc aaatcaaaaa catgagaagc tgttttttct tggctgttct
     7141 tattatttga tgcaggacag ctttttcacg acctctgtaa actataggaa tctgttaaat
     7201 caagggagca gaacttgcct tttacgctac ttttggtaca ataatgtatg attaaaaata
     7261 aactggaggg tttctatctt gaacataggt aagccgagaa aactaggaaa aacaataaat
     7321 gaaatcgagg aaggcgactc gttatcctta acagaatcta ttgaagataa ccagcttttg
     7381 ctgtatttgg gattgacgaa tgatgcaaat cctttgtata tccaacatga ctttgcacaa
     7441 aaaacagaat atcaacagcc aatcgttcca tcagtgatgc tgatggggat catcacaagt
     7501 gcaatttcaa agcatcttcc tggaccagga tcacatgttg tcaatttttc tgtcaatttt
     7561 attgaaccgg ttttccatta tgaaacactg acttttcagt ttgaggtcat aaaagtagat
     7621 aagatgaagg atgtggtgac gatttctgta gaagcggaga atacgaacga agatcgtgtg
     7681 ttggatgctg tcgttatggt ccaaccgcca caagttcctg aattggaaat agcaatcaaa
     7741 agtgaaggtg aattgaatga ataatccaag tacatcaatg tcaggtatca atcgttttta
     7801 tgcaaaagtt tatggatttt taggtatggg acttgccctt agtgcagtca cttcttactt
     7861 ggctctttgg gtatttcctt tgcaattagc aacttttatc aataattttc cactaggctt
     7921 tgtgggtctg tggatcgttg aactgatttt agtaatggtt ctaggcgtaa aagcacaaaa
     7981 gaatccgagc ttagcaatcg gtggatttat tgcctactct cttttgaatg gcataacatt
     8041 agcggttact cttgcatact acgatattgg aacagtaacg caagctttct tgacagctac
     8101 tttgatgttc atggggatgt ctgttgtcgg agtaacaaca aaaagagatc ttagcggggt
     8161 tggaagagca ggacaggcag ctttaatcgg agtgatcatt gcactagtct tgaacatctt
     8221 cattttgaga agtagcgtag ttgagctgtt catctcccta ctgttagtcg tcatctttgc
     8281 agggattaca gcttatgata accaaagcat ccgtaatatc tatgtccaga caaacggaac
     8341 agcacatagt ggaattgctg tattcatggc attgaagctg tatttggact ttatcaactt
     8401 gttcttggcg tttctgagaa tctttggacg gaacaactaa aaaagaaaaa aattcatcag
     8461 ttggggctat accgaatt
//
LOCUS       DACEKH010000104         8425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEKH010000104 DACEKH010000000
VERSION     DACEKH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.108"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            471..2108
                     /locus_tag="IW662_002331"
     CDS             471..2108
                     /locus_tag="IW662_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0867136.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IW662_002332"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IW662_002332"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0867137.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="IW662_002333"
     CDS             complement(3184..3819)
                     /locus_tag="IW662_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0867138.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IW662_002334"
     CDS             complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IW662_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ0867139.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5405..6401
                     /locus_tag="IW662_002335"
                     /pseudo
     CDS             5405..6401
                     /locus_tag="IW662_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6417..>8425
                     /locus_tag="IW662_002336"
     CDS             6417..>8425
                     /locus_tag="IW662_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0867140.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2785 a         1547 c         1293 g         2800 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttt cttctctaaa acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactta caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataagcga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc tattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt ttttttacgt acaagctggc agcttgaata
     5761 ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact tatgctgttt
     5821 cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat aaattatcga
     5881 aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc tgctctactc
     5941 tatttaataa agacccgttt gcattacaga atggacagag tattttatgg attttttatt
     6001 tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc cgctataagc
     6061 ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg atgacacaaa
     6121 tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa ccatattctc
     6181 tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta tctgataaat
     6241 taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact caagttatat
     6301 gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa tttccaaact
     6361 cagggaaggc atggctgata aatatcgggc agttttttat ttgctattta ttagcagtgt
     6421 ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat cgtttcatcg
     6481 aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa agaattcatt
     6541 ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc tttaccttcg
     6601 tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag gttgccttaa
     6661 aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt atgggatttt
     6721 tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct accttgatca
     6781 ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa gagcctgtct
     6841 ttccttcaga tttaaaaata ctgaccggat tgtcagaact attatcgatg gttagcccag
     6901 tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt attattgtac
     6961 aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga tttatcagta
     7021 tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa aattcgcctt
     7081 ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag caaggcgccg
     7141 taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa atcatggaag
     7201 aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac aacacaaagg
     7261 cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc gttctcaatt
     7321 taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct acagacccta
     7381 ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta tccgtcggtt
     7441 atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt atcagtaacc
     7501 tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa ataagtccaa
     7561 atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct gctctttatc
     7621 gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta gacagtccgg
     7681 ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat gattcagcat
     7741 acaaagaaac cttgaaagct ttacaaagca atacagaaac gtcacaattc atccaattat
     7801 ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac tacactgctg
     7861 aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg caaggccttc
     7921 actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc gataaaccga
     7981 ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat gattcgaaaa
     8041 aatatggatt agaacagcac cagacagatt atttcattta taacaacaag tatagtcgtg
     8101 aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt gctatggctc
     8161 ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa gtatctaatc
     8221 atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt aatggaaaac
     8281 aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact gagaaacaaa
     8341 aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt aaccagtaca
     8401 gtgcaacatg ggcgacacaa aaaat
//
LOCUS       DACEKH010000105         8338 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEKH010000105 DACEKH010000000
VERSION     DACEKH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.109"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            264..1457
                     /locus_tag="IW662_002337"
     CDS             264..1457
                     /locus_tag="IW662_002337"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ0867141.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1587..3059
                     /locus_tag="IW662_002338"
     CDS             1587..3059
                     /locus_tag="IW662_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0867142.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3142..3930
                     /locus_tag="IW662_002339"
     CDS             3142..3930
                     /locus_tag="IW662_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ0867143.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3997..4665)
                     /locus_tag="IW662_002340"
     CDS             complement(3997..4665)
                     /locus_tag="IW662_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0867144.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4780..6492
                     /locus_tag="IW662_002341"
     CDS             4780..6492
                     /locus_tag="IW662_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0867145.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6495..8267
                     /locus_tag="IW662_002342"
     CDS             6495..8267
                     /locus_tag="IW662_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0867146.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2553 a         1391 c         1829 g         2565 t
ORIGIN      
        1 gaagatactt tttgagtatc tttttctttt tatctgaaag aatgatttta aaaagcttag
       61 cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa tgtaaaatat
      121 ttttatattt tttaagcaaa aaaatgaaaa aatacgagca gaaaactgtt ttatctgcta
      181 tacttggttc gttgcaagtt cccgaaagga tccgcaggac ggagatgcct tgtaaccgaa
      241 tttaactagg gggaatcatt ataatggtag aaagacactt atttacatca gaatctgttt
      301 ctgaaggaca tccagataaa attgctgacc aaatcagtga tgcaatcttg gatgcaattt
      361 taaaacaaga tccaacagca cgagtagcgt gtgaaacgtc tgtaacaact ggtcttgttt
      421 tagtatttgg agaaatatct acgacggcat atgtagatat tcaaaaagtc gtacgggaaa
      481 caataaaaga aattggttat acacgtgcaa aattcggatt tgatggagat acagcagctg
      541 tattggttgc gattgatgaa caatctcctg atattgctca aggagttgat gaagcacttg
      601 agatccggga tgaagataaa aaagacgtat tagatgagat tggtgctggt gaccaaggtt
      661 taatgtttgg gtttgctgta gatgaaacac cagaattgat gcctttacca atcgctttga
      721 gtcatcgtct ggtacgacgc ttggcagatt tgcgtaaatc aaatgaatta acgtatttac
      781 gtccagatgc aaaatctcaa gtaacggttg aatatgatga tcaaggacaa ccggaacgcg
      841 tagatacaat cgttatttca acacagcatg atgatgcagt agataatgaa acaattcgtc
      901 atgatgtcat cgaaaaagta gtgaaggaag ttattccagc tgaattatta gatgatcaaa
      961 cgaaatatta tatcaatccg actggccgat ttgtcattgg tggtcctcaa ggggatgccg
     1021 gattaacagg aagaaaaatc attgttgata cgtatggcgg ttatgctcgt catggtggcg
     1081 gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc tgccagctat gctgcacgtt
     1141 atattgctaa aaacattgtt gcagcagggc ttgctcgcaa agcagaagta cagctagctt
     1201 atgcgattgg tgttgctcaa cctgtttcga tctcaatcaa tactttcggt actggaacgg
     1261 ttccggaaga agaactgatt gctgcagtga gagaaaactt cgatcttcgg ccagcaggaa
     1321 tcatcgagat gcttgatttg cgtcgtccga tttacaaaca aacagctgct tacggtcatt
     1381 ttggtcgtac agatgtagat ttgccttggg aacatacgga caaagtagat gcgttgaaag
     1441 caagtttagc aaaataagtt atattcagct ggaacgatcg ccaagtggtg gttgttccag
     1501 ctttttttat ggcagttaac ctcttgaaaa agatagtaat tatttatcta tttttagatc
     1561 ctgtcataat gaaagggagg aataagatga aaaaagaaac aaatgttact ttggtcacag
     1621 tgagtatttt cattgcaacg ttcatgacgg ctattgaagg tacaatcgta acaaccgcta
     1681 tgccaacgat tgtcggttca ttacatggaa tggaaatcat gaactgggta ttttcgattt
     1741 atttgctgac aaacgctatg cttactccga tttacggaaa acttgcggat aaagtgggac
     1801 ggaaaccaat ctttattttt gggacattat tgtttattat cggttcgtta ttttgcggtt
     1861 tatctcaaag catgattggg ttgattgctg ctcgtgcgat tcaagggatg ggtgctgggg
     1921 caatgatgcc agtagctttg acgatcatcg gcgatcttta ttctattgaa aagcgagcaa
     1981 aagttttagg tttgaatagt tcagcctggg ggattgccag tgtttttgga ccgcttgcgg
     2041 gaggattcat cgttgaaacg atcagctggc actggatctt ttttatcaat gtgccgatcg
     2101 gaattctatt aattattttg atttggctat atttaaatga accaaaaatc atacatgagt
     2161 ctaaaccaat ggatattttt ggtagtttta gtttgatggc catgcttgtc tcattgctgt
     2221 tagggttcca attattagga gacgaaggaa tgagtccgct tgtttctgct ttgctgctag
     2281 gtagtatcgt aatgttaggt atttttgttt ttgccgaaaa aagagctaaa gatcctgtca
     2341 tcatgatgca tttattcaaa aatccactat ttgttgtggt caacttggct gcagcattgg
     2401 ctagtggttt tttgatggga atcgatgttt atatccctat gtggatgcaa ggtgtattag
     2461 ggctgaatgc agcaattggt gggctggtat tagcaccatt atcactagtc tggatggtcg
     2521 gttctttcct tgcaagcaag tggatgactc aaatgaaaac tcattgggtc ttgagaattg
     2581 gactactaat cgcagctatt gggggcgctt ggttgtatct tatgcctttc agaagtaact
     2641 atttatggtt ttttgctata tcaacggtca tcggtatagg cttaggtgtg accatgacta
     2701 cctcgactgt cagtgcacaa aatagtgtat ctcgtgacca gcttggtgta gcaacttctt
     2761 ttaatacgtt agttcgaaca attggtcaaa cagtcatggt ttctattttt ggtttattga
     2821 taaatggggt aactaaacga gaacttgcag cagcaaattt aacaaatgat gcagatatca
     2881 tgaataaact ggtgaatcca caaacggcta aatatattga tgcggatatt ttgttaccat
     2941 taagaaaaat tctttatgat gggttacatt ccgtttatct agttggcttg ttactcgtga
     3001 tcattgcttt ggtcttaacg cttttcgttc atgaaaaaag tggtaaaata aaacagtagt
     3061 ttcggttaag atatgtacgt agccgaatca ttcaaattta aagttattca aaaataactt
     3121 cttactaagg aaggcgaaaa tatggcattt attcaagcaa atatttattc gaatgtgtta
     3181 gaaatggaag tcaatctaaa tgtgatacta ccgcaaacaa caaaaaaggt gattggcacc
     3241 agttcagagc aagagacaac ggatatacct gttttatatt tacttcatgg aatgggtggc
     3301 aatcatagtg tatgggaaag acgtacgtcg attgaacgtt atgtagcaaa ctacggatta
     3361 gctgtcatca tgccatctac tgatcttggc tggtacacag atacagttta tgacatgaag
     3421 tattggacat tcattgcaga agagctacct gagatttgtc atgaattatt cccgcaatta
     3481 acaagaaaaa gagaaaaaac atatgccgct ggattatcga tgggcggata tggcgcgtta
     3541 aaactaggcc ttgcaaaatc agaaaatttc gcagcagtaa tctctctttc aggcgctgtt
     3601 tctgtaggaa atcgaatgga tgatcttttg atggttcgta aagcatcttt ttgggaaggg
     3661 atctttggac cattggatca aatcgaaggt tcaaaaaatg atccgcaata cttattgaaa
     3721 gaactagtaa aaagcggaaa accagttcca agattttata tggcttgtgg ggaatctgat
     3781 tttctttatc aggcaaatca ggaaatggta tctgctatgc aggaacaagg cttggcggtc
     3841 acctttgaac aaggacctgg cgatcataac tggatcttct gggaccaatg gattcaacgt
     3901 gcattggcgt ggctaatgga agaaaaataa aaaaagagtc ttaaaaagat agctcctata
     3961 gagatcacaa acatagtgat ctctatagga gcttttttac tgctcgtttt ctcttctttt
     4021 attttgttgg cataagcctt caatataggt ttgtgcactc gttagaaaga gtgctttttc
     4081 attcaattct gcatgggact gcatactttc tttaaatccc tctaattgat tttcaactac
     4141 ataattacgc ataaatagaa ctgtttcttt caaaaattca tttccgtcca taattttttg
     4201 tcgaagctgc tgttcatgct gctgatccat taactgtcca atcaataaat tgtggagaga
     4261 ggcaatggcc tgatgacttt gggtgactga aaatccttca cgaacaagta attggatcat
     4321 ttgctccaaa tgttctaatc gactcggaaa agcgggcaca gtattgattt ctaattctgc
     4381 cccacaaggg aaacgtgtat aaaggtcata gtaattttcc atatactgca gcaactgttc
     4441 attccaaggc aaagttggat ctggaagcgc taattcttct tccattgttt cagccatttt
     4501 ttgcaagagc tgttgtttgt ttttgaaata ccaatataaa gcaggtgctt gaacatgaat
     4561 tctgtttgcg atgtttcgca tggaaagctg cgaaagagct tgtgtttcag ccaggatatc
     4621 aaaggcggcc tgaatgatga ttgcttgaga aagctttatt tccattttat caacctcgtg
     4681 tattgcatta tctttttaaa catggtaaat tgttagtcgg tctaatttta cagtgttaaa
     4741 ttagtcctgt caatttaaac aaaggggtgt aatcaacgaa tgaaattaat cttacgacat
     4801 gcgaaaaaat ataaagtagc tgtttttatt tccttgctgt ccgtagcagt catggttgca
     4861 gcagcattat ggcaacctaa gttactacag caagtattgg aagctattat tacagaagat
     4921 aatgatgaaa tgaaaacaat tggtatttac ctgatcagtc tggctttgct aggattagcc
     4981 gcaggtgtaa cgaacacgat tttctctgca aaagtagctc aaggcgtaag tgcagatatc
     5041 cgagaagaag catttcgaaa gatccagaca ttttcatttg gaaatatcga acagttctct
     5101 gctggaaatc ttgttgttcg tctaacaaat gatatcacgc aaatccaaaa tttagtgatg
     5161 atttctttgc aatcactttt tcgaattcca ttcttattta ttggtgcctt cattttagct
     5221 atgatcacga tgccgcaatt atggtggatc atcgtagcgc ttatcgttac ggtatttctg
     5281 attacggcac tttcatttac aaggatgggc aagcacttca tgatcattca aaaactgatt
     5341 gatcgtgtga atagcattgc aaaagaaaac ttactaggga ttcgtgtagt gaaatctttt
     5401 gtacaagaag aaaaccaatt gaagtcattc agcaaagtaa gtgaggactt aacaaaacac
     5461 aacatcatag tcggtacatt attttctgtg atgatcccgt ccttcatgct ggctgccaat
     5521 ttggctgtgg ttggggcgat cttttttgtg agcgatcttg ttaaagatga tcctacgtta
     5581 attggtggaa ttgcttcatt catgaactac ttgatgcaga ttatgatggc aatcattatt
     5641 ggcggaatga tgatgatgat gacttcaaga gcagccgtat cgctgaagcg gattgcggaa
     5701 attttagatg ctgaaccaga tcttacttat ttagacgttc cagaacaaga gttgacgggt
     5761 tctgtcactt ttgaccatgt ttcttttcgt tatcctggtg aggatacaga tacgcttaaa
     5821 gatatcagct tttctatcaa acctggagaa atggttggta tcgtcggggc gactggtgcc
     5881 gggaagtcaa cactagcaca attgatccct cgtctatttg atccgacaga aggcaaagtt
     5941 gaagtaggtg gtgtagatct tcgtcaagta aatgaaaaga gtttaagaca aacggtttcc
     6001 tttgttttgc aaaaagcaat tttattctca ggaacgattg ctcaaaactt gcgccaaggg
     6061 aaaaagaatg caacagaaaa agagatggaa catgcttctt ctattgctca agcaaaagaa
     6121 ttcatcgaaa agttatctga tcgttacgaa gcaccaatag aagaacgtag cagtaacttt
     6181 tctggtggtc aaaagcaacg tttatccatt actcgtggcg ttatcggtaa tccaaagatc
     6241 ttgatcctag atgacagtac gagtgcactg gatgctcgct ctgaaaagct ggtccgagaa
     6301 gcactcgatc gagatttgaa gggaacaacg acgattgtca tcgcacaaaa aattgcttct
     6361 gttgtcaaag cagaccgcat cttagtattg gatgaaggca gattagtcgg cgaaggaacc
     6421 catgaggaat tggtagctgg aaaccgtgtc tatcaggaaa tctttgaaac tcaaaaagga
     6481 acggaggagt aagtatgact gatctaataa aagcaagtaa atttttctat cactatttga
     6541 agcggtacaa actctccttt ttctttatct ttatcacagt gatcattgct acctatctac
     6601 aagtaaaggc acctcaatac atcggggaag cttttcaaga attagctaat tacgtaggag
     6661 gcttgatgca aggcgtagac gataaaagca aatttgtttc tgtaatatgg aaactcttgc
     6721 tgttttatgt actagctagt gcagccaact ttatctatag tattttgttc acacaagtag
     6781 tagggaagtc aacgaaccga atgcgtatcg gtctattcaa caaattggaa aaattgacga
     6841 tccgtttctt tgattctcat caagacggtg aaattttaag tcgttttacc agtgacttag
     6901 ataatattca aaatagtttg aaccaagctt tgcttcaagt catcacgaat gctgttttac
     6961 ttgtcgggat cttgatcatg atgttccgcc aaaatgtaga gttagcttgg gctacaattg
     7021 cttccactcc agtagccgtt ttgatagctg tcttgattat ccgcaaagca cggaaatatg
     7081 tggatcttca gcaagatgaa gtggggaaac taaatggtta tatggatgaa aaaatcagcg
     7141 gacaacgtgt catcatcaca aatggcttac aagaagaaac gattgaagga ttcttagagc
     7201 ataatcaaag tgttcgcgaa gcaaccttca aaggacaagc ttattctggt ctactatttc
     7261 cgatgatgca aggaatgtca ctggtcaata cagcgatagt tatctttttc ggcggctggt
     7321 tagcattaaa tggtgatttg gaacgttcgg tggcattagg cttagttgtg acattcgtcc
     7381 agtattcaca acaatattac caaccactga tgcagatttc atctggatac agcatgatcc
     7441 aattagcgat tacaggggca agacgattga atgaaatatt tgatgaaaaa gatgaagtca
     7501 aaccagaaaa cggtaaagca tttgatggtg ttgaaaaagg tcttgcgttg cagcacgtag
     7561 attttggtta ttctccagaa aaactagtct tgaaagatgt ctcgatcaat gtggacaaag
     7621 gagaaatggt tgctcttgtt ggtccaacag gatcggggaa aacgacgatc atgaatctgc
     7681 taaaccgttt ttacgatgtc aatgatggtt cagtagtcat cgatggaata gatatccgag
     7741 attatgatct tgatgcatta cgtagacatg taggaatcgt tttgcaagaa tctgtattgt
     7801 tctccggaac gatcaaagac aatatcacat tcggcaaacc ggacgcttca gatgaagaag
     7861 tcatcaatgc agcaaaacaa gcgaatattc atgacttcat catgacgtta gaaaaaggtt
     7921 atgatacaga gatatccgaa gaaaataact tattcagtac aggtcaaaag cagttgatca
     7981 gtattgctcg aacgatcatc acaaatccat ctttattgat tttggatgaa gcaacaagta
     8041 atgtagacac ggtaacagaa gcaaggatcc aaaaagcaat ggatgaagca atcaaaggaa
     8101 gaaccagctt tgtgattgct caccggctga aaacaatctt gaatgcagat agaatcgttg
     8161 tcttgcgtga tggggaagtc attgaagaag gcagtcatca tgagttatta gcagaaaatg
     8221 gattttatgc ggaactttat cataatcaat ttgtatttga aagttaaatt aaaagaacct
     8281 cgttataacg gggttctttt tatatatttt atgttgtttg tttttataag gggcagaa
//
LOCUS       DACEKH010000106         8290 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEKH010000106 DACEKH010000000
VERSION     DACEKH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.110"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(45..1511)
                     /locus_tag="IW662_002343"
     CDS             complement(45..1511)
                     /locus_tag="IW662_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ0867147.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="IW662_002344"
     CDS             1898..2494
                     /locus_tag="IW662_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0867148.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="IW662_002345"
     CDS             complement(2552..3034)
                     /locus_tag="IW662_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867149.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="IW662_002346"
     CDS             complement(3140..3337)
                     /locus_tag="IW662_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867150.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="IW662_002347"
     CDS             3632..4390
                     /locus_tag="IW662_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ0867151.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="IW662_002348"
     CDS             4450..4878
                     /locus_tag="IW662_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867152.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="IW662_002349"
     tRNA            4924..4996
                     /locus_tag="IW662_002349"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="IW662_002350"
     CDS             complement(5146..5517)
                     /locus_tag="IW662_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0867153.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="IW662_002351"
     CDS             5732..5917
                     /locus_tag="IW662_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867154.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="IW662_002352"
     CDS             complement(6000..6188)
                     /locus_tag="IW662_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867155.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="IW662_002353"
     CDS             complement(6212..6475)
                     /locus_tag="IW662_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867156.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="IW662_002354"
     CDS             6633..6890
                     /locus_tag="IW662_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ0867157.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="IW662_002355"
     CDS             complement(6961..7152)
                     /locus_tag="IW662_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867158.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="IW662_002356"
     CDS             complement(7293..7613)
                     /locus_tag="IW662_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867159.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="IW662_002357"
     CDS             complement(7636..8070)
                     /locus_tag="IW662_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0867160.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2628 a         1635 c         1314 g         2713 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg
//
LOCUS       DACEKH010000107         8061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.111, whole genome shotgun sequence.
ACCESSION   DACEKH010000107 DACEKH010000000
VERSION     DACEKH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.111"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IW662_002358"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IW662_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ0867161.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="IW662_002359"
     CDS             complement(2944..3378)
                     /locus_tag="IW662_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867162.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="IW662_002360"
     CDS             complement(3395..4600)
                     /locus_tag="IW662_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0867163.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5294..5680
                     /locus_tag="IW662_002361"
     CDS             5294..5680
                     /locus_tag="IW662_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0867164.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5774..5965
                     /locus_tag="IW662_002362"
     CDS             5774..5965
                     /locus_tag="IW662_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0867165.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6479..6811
                     /locus_tag="IW662_002363"
     CDS             6479..6811
                     /locus_tag="IW662_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0867166.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7107..7262
                     /locus_tag="IW662_002364"
     CDS             7107..7262
                     /locus_tag="IW662_002364"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0867167.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7538..8061
                     /locus_tag="IW662_002365"
                     /pseudo
     CDS             7538..8061
                     /locus_tag="IW662_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2664 a         1443 c         1186 g         2768 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtcctt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4861 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4921 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     4981 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5041 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5101 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5161 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5221 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5281 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5341 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5401 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5461 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5521 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5581 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5641 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5701 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5761 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5821 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6061 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6121 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6181 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6241 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6301 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6361 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6421 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6481 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6541 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6601 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6661 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6721 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6781 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6841 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6901 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     6961 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7021 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7081 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7141 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7201 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7261 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7321 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7381 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7441 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7501 taaagtcgac aaaaaccatt caaagtagtg tttcaagatg tcctattcag agtttaccaa
     7561 agagttacta gatattctag acctaaatct tacctttcat gaggatgcct ttcgcaaaga
     7621 gcgcatcaat gacgaaacat gttttgcttt tgatggcaca ttgacctatc aaccggagga
     7681 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     7741 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     7801 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     7861 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     7921 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     7981 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     8041 tgaattcaaa tcagtgaaaa a
//
LOCUS       DACEKH010000108         7826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEKH010000108 DACEKH010000000
VERSION     DACEKH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.112"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(315..398)
                     /locus_tag="IW662_002366"
     tRNA            complement(315..398)
                     /locus_tag="IW662_002366"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..1426
                     /locus_tag="IW662_002367"
     CDS             926..1426
                     /locus_tag="IW662_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0867168.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1616..1960)
                     /locus_tag="IW662_002368"
     CDS             complement(1616..1960)
                     /locus_tag="IW662_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ0867169.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2114..3034)
                     /locus_tag="IW662_002369"
     CDS             complement(2114..3034)
                     /locus_tag="IW662_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0867170.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3221..3712
                     /locus_tag="IW662_002370"
     CDS             3221..3712
                     /locus_tag="IW662_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ0867171.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3736..6111)
                     /locus_tag="IW662_002371"
     CDS             complement(3736..6111)
                     /locus_tag="IW662_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ0867172.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6437..7063
                     /locus_tag="IW662_002372"
     CDS             6437..7063
                     /locus_tag="IW662_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0867173.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7086..7790
                     /locus_tag="IW662_002373"
     CDS             7086..7790
                     /locus_tag="IW662_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0867174.1"
                     /translation="MTETTLYIVRHGKPMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
BASE COUNT         2410 a         1500 c         1361 g         2555 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta
     1381 tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc
     1441 aaaaggaaag ggatttgaat gtcctttatc aatagaacac atttatttaa ttaacttata
     1501 atatagcaga caaaatttta taaacaaaga aaccttttat ctcttttctt ctagaaagga
     1561 taaaaggctt ttttgttttt cctatttgct tctcatagct agtcagccaa gatttctatc
     1621 gtatccccaa cgcaatccgt gcataacggc tcatcttatc tgtcgtccat gctggatacc
     1681 agactagttt gacttctgct ttcgttactt ctggcacttc ttttaatgcg tcatggatag
     1741 attctgttaa aatatctgct aatggacagc ccatagttgt tagagtcatc ttaatgactg
     1801 tttctccatt ttcaggattg aattctatat cataaatcaa gcctaaattg acgatgtcga
     1861 ttcctaattc aggatcaatc accatttcca aggcagcaag aatacgttct ttgattagtt
     1921 ctatttcttg tccagaccgt tcttggtttg cttcgctcat cgtacgacac tccttaacac
     1981 gatttttatt tatcatactc tttttttagc tgcttgtaaa tgagaattta gaagtaagat
     2041 tctcgtttaa atttataaaa atgttcttat tagatgtaat agattaaatt tattctaaaa
     2101 taaacctgat tcctcatttt ttttgtgaac gattttttat ttctggcaat acgccagttg
     2161 ttcgatacat tttttgttcg ttttcatttg caaaccggcc aaatactgaa tgagttggta
     2221 aaatattgat aaatgcatcg tcgtcgattt catgaacgat ctgatcaaga tcatacattt
     2281 cgtagcgggt gatgaccatc atgatcatgt tcccttgttt ttttgaatag ccaccgacag
     2341 acggcagtaa tgtcattcca cgtaccatcc gttttgctag ttcttctgtt acctcttctg
     2401 gcttgacggt aacgatcatt gctgttactt tttgatgact cgtgtggatg gcatcaatta
     2461 cttgagtcat tgcataaatg gaaataatcg tatacaaggc actttcccag ttaaagaaga
     2521 agcctgcgat gactacaatg ataccattta gaatcagcat gtagtttccg actgtttttc
     2581 cagttgtttg tgaaagtaca agagagatga tatccatccc accagttgta aagcccattt
     2641 ttaaagataa tcctacgcca agacctaata aaacaccgcc tactagggca ttcatcaaaa
     2701 tattatcagt gatctgtccg actggaagca gcatcgtcat caccgataca catacaacat
     2761 tcacaaaact aaggatcgtt gctcgttttc ctactttgag aaaccctaat acaaacacag
     2821 ggatattcaa aagaaaaata aatgttcctg tgtctatctt gatatgaaac cattcatata
     2881 aaagcgagga aatgatttga gcgatcccat tcatccctgc agaaaagaca ttggctggaa
     2941 tcaaaaaata atttaaagcg accgcagcgg tcaatccggt tatcaaaata accactattt
     3001 tctttaatct ttcattttta tgatacgcag tcataatttt actcacaagc acctctcccc
     3061 tactttggta ttccaaatgt aagaataaca gtttatctat taaaataaaa tacttcgaac
     3121 tcaaatgaga agaattttag caaacaggta agaatctgcc ctaatcctat gataagatag
     3181 atgtatggta aatatttata tgtatatcag gagggttcct atggcaaaag atgctagctt
     3241 cgacattgta tcagaagtca atacagaaga agtaaaaaat gcgattcagc aaacaacaaa
     3301 agaattgtcc aatcgctttg acttcaagga ttctacagta gaaatcaaat tagagaacag
     3361 caatctagtt gtattgagcg atgatgattt caaaatcgaa cagatcaaag atgtattatt
     3421 tggtaagctg atcaaaagaa atgtgccaat caaaaatatc catttttccg aatcagaaca
     3481 cgctctaggt ggcaaagctc gacaaactgc cgatttggtc aacggaatcg atcgtgaaaa
     3541 cgcgaagaaa atcactacag ctatcaaaaa tgaaaaactg aaagtaaaag cacaaatcca
     3601 agaagatcag attagagtga caggtaagaa ccgtgatgat ctacaagctg tgattgcttt
     3661 attacgtaaa cttgacttac caatcgagtt gcaattcact aactatcgtt aatcaaaaaa
     3721 cgaggctgac gtctgtcatt cctcgttttt ttgatttact gcttcttttg ataaccaaaa
     3781 acgctgtatt ccttcttcca attccccaac acttcgtact tctcttgctg tttgccaatg
     3841 ttgtgggaat aaacgtcgat aagaaaaact agaaaatcgt tgatctgcca atacaacgat
     3901 tccttgatcc gccatgccac gaatcactcg accaccagct tgcaagactt tgttcattcc
     3961 aggaagctga taagcgtaag caaatccaag gtgttccttc tcatcataat atgatttgat
     4021 cagttcctgc tcatgattca tctgcggcaa acctacacct acgatcatac tgccgatcaa
     4081 tcggtcccct ctcaaatcga ttccttctga aaaaatcccg ccaagtacgc aaaatccaat
     4141 caatgtttca gcgggatttg tttgaaattt tgttaaaaat cgttcgcgtt cttcctcatt
     4201 catgtccgtt tcttggatta gcacattgct gtcgggatat tgctgtctga atgcttccac
     4261 cgcaagatct aaatattgat aagaaggaaa gaaaacgaaa taattcccga cctttccatc
     4321 agcaaaagca tgaatcgtct ctactaattt tgccagactg tgttcccttt ttcgataagt
     4381 cgtctctagg taatttgcga tgaaaacttg ctggtttcct tctgggaaag gactcggcag
     4441 tttgtaagcc agactttttt gtccccccag tgtttcctga taataagaga gtggtgaaaa
     4501 actagcagaa aaaagaatac tgctttttcc tttatccaac gactgctcaa gaaacaagct
     4561 tggatcaata caaaactctt ttactatcag atcgcgatac gttttttcga ctgttgtttc
     4621 gtagtgatca tcataaaatt cactgacctt caaaaaatga agcaaatcaa aataataagg
     4681 aagcaattgt tcttgtgatg ggtgctcagg aaattctgcc agccattctt taattttttc
     4741 gcttaattga taacctgcct ttactaaaga ttcagccggt gctttttgat gatgataatc
     4801 ccaatgatct tcttttgcta tttctcggat cgaatcaaat tcttttaata gtttgttgaa
     4861 acggcgatga agttttttga attcctttgg tatagcttcc ttacttcgtt tcgttttttc
     4921 ataagatagt tctgccgaat acatctctcg ggaacgattc accaaattat gtgcttcgtc
     4981 aatcaaaaac agatagtcct cgtttttttc atcttcaaaa aaacgtctta aataaacagt
     5041 tggatcaaat aaataattgt aatccccaat gattacgtca caccagatac tgacatctaa
     5101 tgatagttcg aaaggacaaa gtgtatgctt tcttgcataa gtttcgatga cttcacgagt
     5161 gatctgattc tcatgattca aaagatccca taaaccttca ttaatccgat tgtaataacc
     5221 attggcatat gggcactgat ctggattaca ggtcgtctca tctaaaaagc agatcttgtc
     5281 tttcgccgtc aaagtgacac tttttacttc agctcctgta tcttttaatg ctgtcatagc
     5341 atcttcagct acttgtctag ttattgtttt agccgttaga taaaagatct tttctccctc
     5401 ttcttcccct acagctttca aagcggggaa taacgtagag attgtttttc ctgttccggt
     5461 aggtgcttct acaaacaatt tctgtttcgt acggatcgtt ttataagcag ctaccgctag
     5521 ttcacgctga cctttcctat actcatcatg ggggaatttc aaagccatca aagaggcatt
     5581 gcgtacagtg cgccagtttt cttgaaaaac aagccattct ttataatctt cagtcagctt
     5641 tttaaaaaaa gtttctaact gctctcttgt ctgatgttca acctttctcg tgatcacttc
     5701 ttctgccgtc tggaagtaag tcaattgcag atttatttct tgtaaatcat gttcttgact
     5761 atagatatag gcatacaccc ttgcttgatg aaagaaaagt tccacttgtt ctggttctaa
     5821 atcttcaaat cgtggttcgg aggttttgat ttcatctata taataaatac cgtctctttt
     5881 gaaaataccg tctgcacggc cttcaacgat caggtaatat tcatctaatt gaactgttgt
     5941 ttttaaaaag acttcttttt catagttttc tcccgcatct ttttggagct tgcggtggat
     6001 tctcgctcct tctaatgcag tatggttact ttttttacga ttgtcgatac ttccacgtcg
     6061 caaaataaat tctactaagt gccgcacaga tatttttgac tgtttgatca tttccaccac
     6121 ctctttctct ctaagattac ctgaaaaaac ctagtttagc aaaaagagct ctctttccaa
     6181 acataaaaaa acaccctgct gattaggcag ggatatagga tatagaattt ggatttttgg
     6241 atgaatctaa tgtaacaagg acatattaaa atgctatgaa aacttacttt ccttctcttt
     6301 agaatatcca aatacttttc atatagtctg ataattcaga taagtaaaga gaaataagta
     6361 cctgtttttg tttcctagca atgaatgcgt tatactaatg gatgaaagaa gttttggcta
     6421 tcaaggagga atgcacatgc atatcgaaag attaaaaaca ggaatgattg aagaaaactg
     6481 ttatttggtc tacaacgaag aagcactttt gatcattgac cctggagaag atgcggaacg
     6541 aatcaaacaa gaaatcaaaa aaacaaacca acagccggta gcgattttgc ttacccatac
     6601 tcactacgat catatcggtg cagtggaacc tttacgcgcg tattatcaaa tcccagttta
     6661 tgtcagccca ctagagcaaa aatggctagg tgatccgatt ttgaatcttt ctggactcgg
     6721 tagacatgat gatattgctg atgttattgt ctcgcctgca gaatatgaat ttgaaatgaa
     6781 aacttatact ttaggaaaca tgtcttttcg cgtcgtccct accccagggc attctatagg
     6841 aagcgtcagc tttatttttg atgattttgt cgtttctggc gacgccctgt ttagaggaag
     6901 tatcggtaga accgatctgt atacagggaa tttagaacaa ttacttcata gtattcggac
     6961 ccagctgttt gtattacctg atgaatttgc agtctatcca ggacatggcg atgcaacgac
     7021 gattgaacag gaaaaaagga caaatccttt tttcaatcgt taaaacgact taggaggaga
     7081 ataaaatgac agaaactaca ctttatatcg tacgacacgg aaaaccgatg ttcaatacaa
     7141 ttgaacgagt acaaggctgg tgtgatacac ccctcacaaa gcaaggacaa gaaggcattc
     7201 attatttagg tcgcgggtta aaaaatgtcg acttttcatt tgcttattct agtgacagtg
     7261 gtcgtgcaat cgaaactgcc cgtattattc taggagaaca ttctaaggga aaagaaattc
     7321 cttattttat tgaaaaaagg attagagaat ggtgtttcgg gtctcttgaa ggtggatatg
     7381 acatggaaat gtggggtgtg atccctcgtg tattgaattt taaaacatat gatgaaatgt
     7441 ttaccacaga agtttctttt gaacaaattg ctaatgctgt atacaactta gataccgctg
     7501 ggtgggcgga aacttatgaa acattgcgag accgtgtatg gacaggattt gaagatatcg
     7561 cccatcactg cgaaaaaaat ggtggtggaa atgttttagt cgtatctcat ggtttgacga
     7621 tcgccttttt gcttaatctg atcgatccta aacagcctgt tcgtgctggg cttttaaatg
     7681 gcagcgtaac aaaggtcact tatgcaaacg gtcgattttc tatccacggc atcaatgata
     7741 cgtcttatat cgataacggg aaaacacata gtcgcgactc gtttctataa tggggatatt
     7801 ttaattttta ccagagaatc ataaaa
//
LOCUS       DACEKH010000109         7534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEKH010000109 DACEKH010000000
VERSION     DACEKH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.113"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="IW662_002374"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="IW662_002374"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ0867175.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3273)
                     /locus_tag="IW662_002375"
     CDS             complement(2032..3273)
                     /locus_tag="IW662_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0867176.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="IW662_002376"
     CDS             complement(3291..4214)
                     /locus_tag="IW662_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0867177.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="IW662_002377"
     CDS             complement(4435..6927)
                     /locus_tag="IW662_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0867178.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="IW662_002378"
     CDS             complement(6934..7401)
                     /locus_tag="IW662_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ0867179.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2075 a         1638 c         1208 g         2613 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatc
//
LOCUS       DACEKH010000110         7047 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEKH010000110 DACEKH010000000
VERSION     DACEKH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.114"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..104
                     /locus_tag="IW662_002379"
     CDS             <1..104
                     /locus_tag="IW662_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0867180.1"
                     /translation="FQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(172..465)
                     /locus_tag="IW662_002380"
     CDS             complement(172..465)
                     /locus_tag="IW662_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ0867181.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(493..1158)
                     /locus_tag="IW662_002381"
     CDS             complement(493..1158)
                     /locus_tag="IW662_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ0867182.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1298..1930)
                     /locus_tag="IW662_002382"
     CDS             complement(1298..1930)
                     /locus_tag="IW662_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0867183.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1937..3004)
                     /locus_tag="IW662_002383"
     CDS             complement(1937..3004)
                     /locus_tag="IW662_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0867184.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(3001..3732)
                     /locus_tag="IW662_002384"
     CDS             complement(3001..3732)
                     /locus_tag="IW662_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ0867185.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3900..4379)
                     /gene="greA"
                     /locus_tag="IW662_002385"
     CDS             complement(3900..4379)
                     /gene="greA"
                     /locus_tag="IW662_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ0867186.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4515..5690)
                     /gene="mltG"
                     /locus_tag="IW662_002386"
     CDS             complement(4515..5690)
                     /gene="mltG"
                     /locus_tag="IW662_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ0867187.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5855..7015
                     /locus_tag="IW662_002387"
     CDS             5855..7015
                     /locus_tag="IW662_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0867188.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
BASE COUNT         2086 a         1486 c         1105 g         2370 t
ORIGIN      
        1 attttcaaaa gaaattaatg gctatttttt taaccaatct gaaaagctac tttgccaccc
       61 atgagcttca aaccaatcaa gtaaaattgt ctgcaggata ttaatagctg aaaacaatat
      121 tttcaaaaga aaaaagagaa ggtgacaaaa attgtgtcac cctctctttt tttattgttt
      181 tttaaagtga taggttggct cctccaggtt gggatcataa tcaacaagta aatcatatcc
      241 tttaaagaac cattcatctg cttcctcgat aaaaaaaagt gtcccatcca tttccacttt
      301 ctttacagga tgttctggct gttcaactga cattccaatc gaaaaaccat catgaacctc
      361 tgttttgccg tagacttttc caaaaaaacg tattccgtaa gtttctggca gatcaatttc
      421 tgagtcaaac cacttttgtg cttttggcgt taccgttaat ttcatcccaa caacctactt
      481 tctcttttct ttctattgat tcatcttatc gtctagaata tccacatcgc ctgtctctcc
      541 aataaccagt agattatcat tttcacgaac atattcatct gctgcaggag aagcgataac
      601 cgtttgatct actcttcgta tggcgactac tgttaagtca aaacgttgcc tgaaatttaa
      661 ttcagctaat gttttgttga aaaatttagg attggagact cggatttctg ctaaagaata
      721 tttatctgac agctctaagt agtctagaat attctttgat actaagttat gtgcaatccg
      781 tatccccatg tctcgctcag ggtgtacgac acgatctgct ccgatcttct ctagtacacg
      841 ggcatggtac tcattttggg cttttgctaa aatattcggt acgcccattt cctttaccat
      901 cagagtaact aagatgctgg cttggatatc ttctccaata gcgacaatta catgatcgaa
      961 atttcttata cctaatgaac gaagtgtcat ctcatcctgt gcatttgcca caacagcatg
     1021 ggtggctata ttcatatatt cattgactcg atcttcatta ctgtcaatag ccagtacttc
     1081 ttgtccagct tcgatcagtg tacggcagat gcttccgcca aatcgtccaa ggcctacaat
     1141 tgcaaaattt tgtttcacca ttttcctcct ttagtatctt cttttctcag tataacaaag
     1201 cctttccaaa aacaaaagca agcatcatta ttgtaaaaaa gccaagaaaa ataatagtta
     1261 ttttctcagc ttttgattat cctctttatc atcgttttta tttcaccaaa ccatgtttaa
     1321 acgcatagat agctgcttga gttcggtctt ctacctctaa cttagctaat atattcgaca
     1381 catgcgtttt gactgttttc agtgtgataa acaattcatc cgcgatttcc tgattacttt
     1441 tcccttcaga aatcagcatc aaaatttcat tttctcgatt cgttaattct tcatgcaata
     1501 ctggatcatt tcgccgactc attttttcca tcatttttgt tgtcacttca ggttctagta
     1561 ctcgctcgcc gcgttgcgta gcccttatag catcagctat ttcatgtgct gtagacgtct
     1621 tcaacaaata tcctgctgct cctgcttcaa ttgcaggata aactttttca tcatcaataa
     1681 aacttgtgac gataatgatt ttggcttttg gccaatcttt taagattgct tttgttgatt
     1741 cgatgccatc catttcttcc atgactagat ccattaaaat gacatccggt cgaagtgcca
     1801 tcgccttttc atagccttga cgtccatttt cagcttctcc aatcacttca atgtcttctt
     1861 ggatagataa ataagaagag acgcctaagc gtaccatttc atggtcatct actaataaaa
     1921 cttttatcat ctttatctat gcctccttca tcaaagggac cttggtctcc acgctcgtac
     1981 cttgcccttt aaaactaatg atttttactg ttccaccaat accagctact cgttctttga
     2041 tattgttcaa tccataactt cccgttttgg tttcattcat attaaatcct gtcccgtcgt
     2101 caatgatacg taataaaaga ttgttatcta ttttatgcaa atagacttct aattcattag
     2161 ccttagcatg tcttaatgtg tttgataaca attcttgaac gattcggaac aagtgatcct
     2221 caatggaact agttaatttt acatcttcta cctcccattt tagcgaaatc tggattttat
     2281 tctgcaattc cttcaataac tgctcaatgc cttgttttaa acttttttct tctaaattga
     2341 ctggacgcag atgaagaagc agcgcacgca tctcagactg ggaagcgttg atgatctctg
     2401 ccaccatttt caactgctta cggaacattt ctggtgtctc acttttttct gcttgctccg
     2461 tcaatgccga catcatcatc atcgctgcaa ataattgctg tgaaactgaa tcatgaagct
     2521 cacgagctaa tcgatgcctt tctaatttta aaatctcttc tttggtttgt ccatctacat
     2581 attgaggacg gctagtcata atctgtaatt cactggatac ttcgatcatt ttttctttta
     2641 ttttagtaat ttccatatca atatctttga tgtacagatc atccgacgcg cttgggatcg
     2701 gctgatgcaa aagtttggat tcataattgc cagaagaaag tgcacgaagc ttttcttcga
     2761 ttttcccgta ttttgccttt tgaataagtg ataacaatag aaatacgatc aatcctacac
     2821 ctaatgaaat tgccatcaaa tggaagataa gcggaacgta taataaacgc gcacgcagca
     2881 attctcccca ccaatgactt tggttactgg cataaaaata agtgaaaagt gaaaatagaa
     2941 taataaggaa agccgcaatc cccgaataaa cagctagcat tgctttggat attcttccca
     3001 tcatattcgt atcacctcga catctccaac caaagtattg gtaatgatct tcaatctgcg
     3061 aggattttca tcataatcac tgctgcagat tttgatctgt tcgtttttta aggaaaactg
     3121 ctcttcttca aacaagacat ttcctactaa tgtcgcatgc tctaaacgaa tagccactcc
     3181 caggggtact aagatccgtg ttctgcctat cccttttctt acaatcacaa tattatcatc
     3241 ttttggcaaa agtgtattgc ccaaatcgat gatagtatca ccagaaatga tggcaatgtt
     3301 gatatcatcc cattcataga cgtcgtttcc aatacgttgg ttaccaaata attgctgtct
     3361 tttacgctca ttattatgtg tttttaattg ctctgtttgg accatcatga tctgtttttt
     3421 tcgccaaaaa gcattttttg tcaaatctat tcctgaaatc tctacacctt tcagcccgat
     3481 aaataaaacg gccaatacaa ccatcatcca taaagcagga ctgttgacta agctaaaaaa
     3541 aatgaccaag ctccctaaga tcaattgaaa attttgaaat ttagtacgag gatatttgcg
     3601 catagctaga tatatattga aaataccgaa tattactaaa agcaaaagtc cagtattgtt
     3661 tacgatttgc caaacagcaa ataacagcag cagtgcttcg actaccacaa aaaaacgcca
     3721 agaactattc atcgcttttt ctccctcctg acacattctc atttcatcat acctattttt
     3781 ttgactgttg gctataggac tttagatgta agatcgttca gtgaaacctg caatactcaa
     3841 agagaaaaga tctagagtgc gtcatcagta agatgaactc tctagatctt tcagtcagac
     3901 tacgcttgtt ctactttgac gattttaact aacatatcgc cacctggtgt agcaatggtt
     3961 acttcatcat ttttacgttt gccaatcaat gcttgcgcga taggagaatc attggaaatc
     4021 tttccagaaa acgggtctgc ttcagcactt ccgacaatcg tgtactcttc ttcatctcca
     4081 tcaggcaact cgatgaacgt aactgtttta ccgatcgata cctcatctgc atctacgccg
     4141 tcattatcga tgatttgtgc aaaacggatc atattttcaa ttgttgtgat tcttccttca
     4201 acaaatgctt gttcatcttt agctgattcg tattcggagt tttcggaaag atctccgaaa
     4261 ccgcgagcga ttttgattcg ttcaatgatt tctccgcgtt taactgtttt tagttcttct
     4321 aattcttttt ctaatttttc tttaccttct agagtcattg gataaacttt ctctaccatg
     4381 tgtaacgtct ccttttttcg ataaaatacc gcaaaaggta atcttttgag gaatatagtc
     4441 caaaaaagat taccacgctt ttcttaaaat gtaaatgttt ttctaaaaat gatcaatgac
     4501 tagcccctgt ttttctaaga attatttaca tatttttgca ctagttccat atgttcttca
     4561 tacgttttag cgaaataaac atttccagtg ctcgtatctg ctacgaaata ataatagtca
     4621 ttttctgctg gttctaatac tgccttgatt gcctcttcgc ttggattgtc aaaaggacca
     4681 ggcccatagc cggtattggt atacaaattg tatggtgaat ctacagctgt atcttcatac
     4741 gtcaccaatt ctttatgttc tcctaatgca tataaaacag agatatctga ctgaagtggc
     4801 atatttgcct tgattcggtt aaagaagact tgtgcaatat ttttacgatc attttctttt
     4861 acgccttctt tttcaactag agaagccaaa gttaaaactt cttgtacagt caaatttttc
     4921 tgtttgatag ctgaataata gttttgcatg accgtatttg ttttgttcac catctcaatc
     4981 acaagatcct taagcgaagt actcgtgtag taatcataag tagctgggaa taagtagcct
     5041 tccaaacggt attttgtgtt ctcagcttta gaggcacttt ccaacaattc tgggaatttt
     5101 tgatacaact cgttaaagaa agtctcatca tttactaaat ctaaaaattc tttcttgtct
     5161 ttaccagtta ccttcgctac ccgctcagca atctgatcga tgtcgtagcc ttctggaatg
     5221 gcaattttcc catctgcaac ttttgttggc tcagatgtgc cgccttcttg caactgcttg
     5281 ccaatctcat caagagtcat attaggcgct aactgataat aaccagcttg aaagccagtt
     5341 aagtttttaa attttgtata ataattgaaa acaatcccac ttttaataat attgtctttc
     5401 tccaaaattt cgccaatctg cttattagaa gagccgcttg ggatttccac ttggactaat
     5461 tggtcgtccg atttatctaa aggcttcaac ccgctatcta cataacgata tacggtaaac
     5521 ccgagaaatc cgccaataat caataatgta agagcgatca ctaaaatgat cttcctcacg
     5581 atcttatctt cttttttacg cagatttctc cgcttgtcga ttatatctgc tgtagctgcc
     5641 ttactggctt cttttttgtc tttgcttggg tgttgtttgt ttcctgacaa aacaactcct
     5701 ccttactatc atcttcgtaa ctctgtttat tatacatgaa atttctactg ccgtgaatta
     5761 ttttaccaaa tattatgtca aaattgtaag tggcaaatgc tctttttgag gattacaatg
     5821 aaaaatagtt aaaaatggat aggaaggatt taatatggca aaaggaaaac atcaaaaaaa
     5881 acagcagatc tctctttctt ttctcgcagt tttgttgctg gctgtcagct tcttttcaac
     5941 aggcatcatt acaggatggc aattaacaaa acaacctcaa ccacaaattt ataaaacgga
     6001 acccgcacaa cttgcttcca atttaacatc taatgagcag atgcttgcca gcaaaattga
     6061 tcaattactg caaaaatcag attatatcgg ttcgatttat gtacgcaaaa ataatcgagt
     6121 gatattagaa aaaggatatg ggtacgcaaa tctacgcata aaagaatcga attctccttc
     6181 tctttactat caaatcggtt cgatccaaaa agcgatgact gctttactta tcctaaaaca
     6241 aattcaatct ggcaagataa cttttgacac aaagctatct gaattttatc ctcagattcc
     6301 tggaagtcag caaatctcaa tcaaaaatat gctttacatg cgctctggtc ttcatcgaac
     6361 cgcttctcca aaaaaaccta tgtcagatag tgagatcatt cagtttgccc ttcatcattt
     6421 gaaatttact ggttatgata cgtatcatta tgaaccatta aattatacat tgcttacagg
     6481 ggtattgatc aaactaatga atcagcctta tgaaactctt ttaaaaaaag aaatcattcg
     6541 gccactccat ctagaacata cagattttta tgaaaatgta aaaaattccc ctcagcatgc
     6601 tgtatcttac caaatgtctg caactgacga ttattcaaaa gcattggtcg agcctgaaac
     6661 agacatccga aatgaattag gcacaggaaa tataagtatg tctgtttatg acttgaacaa
     6721 attctttacc agtgttctgt ctggcgatat tataccaaaa gaactacttt tctcattgtg
     6781 gcaaaataat ggtgataaac atccttacag cggcggtgtt tacagtggaa atgattatat
     6841 ccttgctcag ggaaatatca atcgattcca tgcagtagcg gcgatgaaaa aagacacaaa
     6901 agatgcaatc gtcatggaaa gtaatatcca atctgataaa aacatcaaat tgccggccac
     6961 tgatttaaga aatcagattt acgaactgat ggaaggtgtt aatttaaaaa gctaagtcga
     7021 gacaaaaaaa ccagcaggat gctggtt
//
LOCUS       DACEKH010000111         6934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEKH010000111 DACEKH010000000
VERSION     DACEKH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.115"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            24..1178
                     /locus_tag="IW662_002388"
     CDS             24..1178
                     /locus_tag="IW662_002388"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ0867189.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1200..1952)
                     /locus_tag="IW662_002389"
     CDS             complement(1200..1952)
                     /locus_tag="IW662_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0867190.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2316..4982)
                     /locus_tag="IW662_002390"
     CDS             complement(2316..4982)
                     /locus_tag="IW662_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0867191.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5323..6774
                     /gene="cls"
                     /locus_tag="IW662_002391"
     CDS             5323..6774
                     /gene="cls"
                     /locus_tag="IW662_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0867192.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2047 a         1433 c         1261 g         2193 t
ORIGIN      
        1 atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc ttttttattc
       61 ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat cctgcaaaat
      121 accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa gacatcatta
      181 cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag ctaatcgaca
      241 tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg gtaattatcc
      301 accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag gcttgctacg
      361 gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca gaacgaaaag
      421 tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt gaagtgacgc
      481 aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta tcgattgaag
      541 acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat tatagcgaat
      601 ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc caaaaagttt
      661 ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg attgctttcc
      721 atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac caaacagacg
      781 aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac agccgacgaa
      841 tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg gaaaattcga
      901 aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt gcagtaagtg
      961 aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca caatctcatc
     1021 aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg attttcagtg
     1081 agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct ttttcgatta
     1141 caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa aagaaccctt
     1201 cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct ataggtcagc
     1261 ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta cctggtcaca
     1321 acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt aattgattcc
     1381 tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat taagtatgtt
     1441 tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat tcctagttgc
     1501 ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat cacgcaccat
     1561 ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc tggatttgct
     1621 gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa cgactgtttg
     1681 ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt ttctagcatt
     1741 cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga aagaaatctg
     1801 ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc cgttttcaga
     1861 taatttttta catcttcagc aatagttact tgcgattctt gctggatttc ataatattct
     1921 tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt ttgttatatg
     1981 ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct tttgatgaat
     2041 aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga ccaaagtcgt
     2101 gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag tattagctca
     2161 aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc ttcggtcaca
     2221 agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt gtgactcaga
     2281 attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg tactcgtaca
     2341 atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc catacatgta
     2401 aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag tcctgggatc
     2461 attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact tctgttagag
     2521 aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt ttgcaatgtc
     2581 cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga atgctgcatg
     2641 ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata cgttacagaa
     2701 atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg gttcatcgta
     2761 gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt gactaaattg
     2821 atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa ggcaaagatg
     2881 atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt cttgatatta
     2941 tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt atcatccgtc
     3001 aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc aattccgatg
     3061 tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc tgagatatga
     3121 cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac acgtgcataa
     3181 acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc tagttccgtc
     3241 ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat agcaacagct
     3301 gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct tgcttctgtt
     3361 accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc aacaaggatc
     3421 aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta agcaaaagct
     3481 agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt tttcatcaat
     3541 tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg ttggaagacg
     3601 atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg tccagtagac
     3661 attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt gtttcggatt
     3721 tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc cgtagggtcg
     3781 cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact gatttcgatc
     3841 aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc agtatcgcca
     3901 ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc agtcttatct
     3961 gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg aataatggca
     4021 tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat agactgtaat
     4081 gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct gacgatatct
     4141 gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat acctaaaatc
     4201 aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc cagacctcgt
     4261 tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg tccgatctcc
     4321 gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac gatcgttcca
     4381 ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc cgttacttct
     4441 ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa tgatcccgca
     4501 tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac gatatctcct
     4561 accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac tcgagcatcg
     4621 ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt ttggacaaca
     4681 ctgattatag aattcaacat caatactgca aaaataacta agaattcgat gtagtcaccc
     4741 atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc tttgaaagtt
     4801 tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt caagccatct
     4861 ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct ctgcgtttgc
     4921 tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc cttgttttcc
     4981 aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa aagagaccta
     5041 agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa aaccatttgg
     5101 ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta aggagatatt
     5161 caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta gcccgattta
     5221 cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc tttcttctat
     5281 catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc tagtattata
     5341 accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat caaaccccga
     5401 gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg ggtgggcttt
     5461 gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta tctccgacaa
     5521 agtgacttga aagaattaga aaactttcag tcttttagag gagataccat tgaacattac
     5581 gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc ttcattaaat
     5641 cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca agagaaattg
     5701 gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga atattacgca
     5761 tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa agccgcagaa
     5821 ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa agcaaaagat
     5881 ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc acaaagggca
     5941 ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat cgatggaaag
     6001 attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac aaaaaaattc
     6061 ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt gctccagctt
     6121 cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg ctatcatttg
     6181 aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac atccatccag
     6241 cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt tatcaaattg
     6301 attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc tgatgaaagt
     6361 gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat catgattccc
     6421 aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg gcagctgatc
     6481 aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa aacattgata
     6541 atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag ctaccgattg
     6601 aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact tgctactcag
     6661 ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa ggaaatgtct
     6721 aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct ataatttttt
     6781 tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat cttgtcacag
     6841 tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt
     6901 caacaaacat gagaagctgt tttttgaact tgtt
//
LOCUS       DACEKH010000112         6857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEKH010000112 DACEKH010000000
VERSION     DACEKH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.116"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            127..579
                     /locus_tag="IW662_002392"
     CDS             127..579
                     /locus_tag="IW662_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867193.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IW662_002393"
     CDS             593..1282
                     /locus_tag="IW662_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867194.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IW662_002394"
     CDS             1407..3758
                     /locus_tag="IW662_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ0867195.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="IW662_002395"
     CDS             3838..4233
                     /locus_tag="IW662_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867196.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="IW662_002396"
     CDS             4558..5175
                     /locus_tag="IW662_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0867197.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="IW662_002397"
     CDS             5216..5515
                     /locus_tag="IW662_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867198.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="IW662_002398"
     CDS             5549..5716
                     /locus_tag="IW662_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0867199.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6324)
                     /locus_tag="IW662_002399"
     CDS             complement(5761..6324)
                     /locus_tag="IW662_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0867200.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(6352..>6857)
                     /locus_tag="IW662_002400"
     CDS             complement(6352..>6857)
                     /locus_tag="IW662_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0867201.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIK
                     VLMGITA"
BASE COUNT         2309 a          989 c         1356 g         2203 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttcggt
     6301 tctgttgcga agtttgaaaa tcaaacgcgc cctctctgaa ctccaaatat cttaggctgt
     6361 tattcccatt aataccttga tttcagtaga caccgaaaag ccgaagagcg ttccatttct
     6421 tcggttcttt ttatatattc ctcgaatggt ctccatgccc ttaatcgtgg aagaggctgt
     6481 acggagactt tgataaaatt tatttcgtcg tttaataggt cgatggtctt gttctattaa
     6541 attgttaaga tacttcacag ttcggtgctc tgtcttagta tataaaccca cactctgtaa
     6601 ctttctaaag gcggagccaa gagaaggtgc tttatcggtc acaattgctt tcggctcacc
     6661 aaactgttta tggagtcgtt ttaagaaagc ataggctgct tgcgtatccc gtttctttcg
     6721 taaccagata tctaaggtta agccgtccgc atcaattgca cgataaagat aatgccaacg
     6781 tcccttaatt ttgatatagg tttcgtccat tttccatgaa tagaaggatt gtctattttt
     6841 cttcttccaa agataat
//
LOCUS       DACEKH010000113         6845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.117, whole genome shotgun sequence.
ACCESSION   DACEKH010000113 DACEKH010000000
VERSION     DACEKH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.117"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            238..1629
                     /locus_tag="IW662_002401"
     CDS             238..1629
                     /locus_tag="IW662_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0867202.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            1688..2491
                     /locus_tag="IW662_002402"
     CDS             1688..2491
                     /locus_tag="IW662_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0867203.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2524..3378
                     /locus_tag="IW662_002403"
     CDS             2524..3378
                     /locus_tag="IW662_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HAQ0867204.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3375..4076
                     /locus_tag="IW662_002404"
     CDS             3375..4076
                     /locus_tag="IW662_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAQ0867205.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4084..5151
                     /locus_tag="IW662_002405"
     CDS             4084..5151
                     /locus_tag="IW662_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0867206.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5144..6109
                     /locus_tag="IW662_002406"
     CDS             5144..6109
                     /locus_tag="IW662_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0867207.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTFEDAYIIIDLLFQTEKIVVSNEEKYFYLQRNDSIMGKSFSM
                     NNFDVIEAWQYNKEKILDAFPDLHDSYYRRLCWAYFYVLDKMVLSSSYQQIPRKKTVI
                     EFLKKNRNFILTYKGFTKSRKIAIIALSFRVDWYRKLVEQKNKKN"
     gene            6154..>6845
                     /locus_tag="IW662_002407"
     CDS             6154..>6845
                     /locus_tag="IW662_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0867208.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENE"
BASE COUNT         2473 a          952 c         1313 g         2107 t
ORIGIN      
        1 tatatgaata ttgtaacttg tgaataagga attagcagga gaatactagt tcagcataaa
       61 ggaagtgatg ggacttctga gacaactttc tgttatgcag aaatacacat ataaacagac
      121 aataaacgct tttccccgtt tcttttaatt tcctttacaa tttggttatt cactaggttt
      181 ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag tgtatatatg
      241 aataaaaatg gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt
      301 gtattaaatg tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg
      361 agaaacttag aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg
      421 aatattttgt taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt
      481 actgttattg gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga
      541 tggtttgcat ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt
      601 agtgtttacc ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc
      661 cttatcgtgg gtttggagaa agatgtcgca ccagctatcc aaaattttca atctaagaaa
      721 aacaataaac ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa
      781 ttgatcgatg atatggatat cgtctatttg gcttctaaaa tcccagaaga tgaaaaaatc
      841 aacatttatc aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag
      901 aatctgacaa tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct
      961 tctgggttca gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt
     1021 tcgcttattt tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa
     1081 acaacttcac ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa
     1141 ttttcaatct ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc
     1201 ttggctaaaa gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt
     1261 tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca
     1321 aggccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta
     1381 agacacaatg tacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca
     1441 gattataaca gtaaactacg ttttgatttg ttgtatatca aaaaatattc attactatta
     1501 gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc atctcaagga
     1561 ctagatgaag atgaaaatat tagttcatta gcagaaatcg acttttcaca agagaaaatt
     1621 tatcgctaga ttggaaaaat tttgagcaaa gaggtaggag agaacattgg aaaataaaaa
     1681 tgtaagtatg ctgctttctt tatatgataa agaaaagcca gagtatttaa aggaagcatt
     1741 aactagtatt tttgagcaaa cagtaatgcc aaaagaaatt gtattagttt atgacggccc
     1801 tataaatgaa aaacttcaag tagttgtcac ttatttccaa caaaaagatc ctcacttgtt
     1861 tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa ttggattaga
     1921 acatacgaaa aatcgcttag tagccagaat ggatacagat gatataatgg aaccccaacg
     1981 attagaaaaa caattagaga tcttccaaaa agatccagaa atagcgattg ttgggagtaa
     2041 tattgaggaa tttactggca cgcttagcaa tgttatcgga aaaagaattg tacctgaata
     2101 taacaatgca attcgtgtat tttctaaaag aagaaatccc tttaatcata tgaccgttat
     2161 gtttgataaa aaagctattt tggaagtggg aaattaccaa ccattaagtg ggtttgaaga
     2221 ttattatctt tgggcaagat tattaaaggc aggttataaa ggatataata ttcaagaaac
     2281 attagtctat gcgcgaactg gcaaagatat gtatgcaaga agaggcggaa tgaaatatct
     2341 tggttctggt cttatgggtc gttatcgtat ctggaaagac ggtttagggt cagtaaaaga
     2401 ttttataatt gtttcttgcg gacatatttt tattagctta ctaccaaatc aattaagagg
     2461 aaaattttat caaacgcaat taagaaaata ggaggttttt ctttgtatag cactgatcca
     2521 aaaatgaaaa aaatacaaca gattacgttg aaaatgtctc aagagattgt tgatttttgt
     2581 ctaaaaaatc aacttctttg ttatttctgc ggtggtggtg ccatcggtac tgtgagagaa
     2641 caagggttta ttccatggga tgatgattta gatttcttta tgcctagaga ggattatgaa
     2701 aaattttatc aactatgggt taattcagaa aaaagtaaaa aatatcccat acagaaggct
     2761 tctaaaaatt ataatgatca taattctttt acaactatcc gcgatgcaga tactacattt
     2821 attaaaactt atcaaagaga tttagatata gtccatggaa ttactattga tattttcccg
     2881 ttagatgttg cccctgtttc gaaatttagc cgtaaaattc aaaaactatg ggctttagta
     2941 tatgctttat tttgttctca agtcgtccct gaaaaacatg gtggaataat ggctacaggt
     3001 agtaagatac tcttatctat tttccgctca cctcgtacga gatataagat atggtctttt
     3061 gctgaaaaac aaatgacaaa atataataat gaaccaacgg gctatgtgac tgaactttgt
     3121 gtcggaccta aatacatggg aaatgtttat cctgcaaaag actttgaatc tgctgtgtgg
     3181 gtgccattcg aagatacaga gatgccagta ccagttggtt atgataatta tttaagtcaa
     3241 gtttttggtg actatatgca gcttccgcct gaaaaagatc aggtttctca tcatgaagca
     3301 gtttatattg atccagaaca tccatacaca atgtacaaag gaaaatatta tttaactaaa
     3361 ggagcagaaa aaaaatgatt acagctatta ttattgctgg tggtgtgggt aaacgaatgg
     3421 gacaagatat cccaaaacag ttcattacaa ttgaaggaaa acctattatt atctatactt
     3481 tagaatcatt tcaacaccat cctcaagttg accggatttt agtcgtttgc aaatctggtt
     3541 gggaagaaac gatgtgggcg tatatcaaag aattcaatat tagcaaagtc aaatgggtca
     3601 ttacaggtgg atcaaaggga caagagtcga tcaataatgg tgttcagttt ttgaaagact
     3661 attctgaaga tgatgatacg atcatcatcc atgatggaat ccgtccacta gtagacgaac
     3721 tggtattgtc agatgtaatc gttaaatgta aagaatttgg taacggtgta acctctctac
     3781 cttacaacga acaaattttt atcaagaaaa cagaagaaac gacggaacag tatgtcgatc
     3841 gtaacacatt gaggcgtgtc tcaactccac aagcttatca atacggaaaa ctacgggcag
     3901 catatgaccg ggcagtgaaa gaagacatcg gtatgactga ttcttcttat acgaatacca
     3961 tgatggtaga tttgggagaa actttatatt ttgctgctgg ttctgataaa aatatcaaat
     4021 tgacaacaac agatgatcta gaattgttca aagcatattt aaaaatgaaa gactaggtga
     4081 aagatgatga cgttgattaa acaaccaatg taccaagaag atttggcaaa agtaatcaat
     4141 catacaccaa aaataaaaca gctacatgga aaatccgttc tgatgattgg tgcttctgga
     4201 atgatcggct cttttttgat tgatacgttg atggctgcta atgaacaact aggcatcaaa
     4261 atcaaagttt atgcaatggg cagaaaccgt cagaagcttg aaaaaaggtt tgccagttat
     4321 cttgaattag atactttcga aattgttgaa ggtgacgtca cagaaccctt accaaaagaa
     4381 attcaagctg actatttgat acacggtgca agtaacacac atcctaaggc ttacgcaact
     4441 gatccaatcg gtacgatcat gactaatctt gcaggtacag agcaagtatt gaaacatgca
     4501 gtagcaaccc aaccggttag agtattattt ttatcaacag tagaaatcta tggcgaaaat
     4561 cgaggcgata tagaaaaatt cacagaagat tactgtggat acatcgattg taatacactg
     4621 agagcaggct accctgaagg aaaaagagtc agtgaatcat tatgccaagc ctacatcgca
     4681 aaacacgacc tcgacatcgt gattccaagg ctttgccgta cgtttggtcc gacgatgctt
     4741 ttatctgata cgaaagcatc ctcacaattt attttaaatg cagtcgaaca aaaagatatt
     4801 gttttaaaaa gtgaaggaaa ccaatatttt tcgtatgtat atgttggaga tgctgtttct
     4861 gctattcttc atttactgct tgaaggagaa aaaggagagg cgtacaacgt ttcttcagaa
     4921 aactttgatt tacgactaaa agaattagcc caacagctag ctgatatcag cggcaaacaa
     4981 gtaatttttg aattgccgga tgagattgaa caaaaaggtt tctcaaaagc tagcacagca
     5041 gtacttgaaa ataaaaaaat taaagagaca ggttggaaac caatttttcc tttgaacgaa
     5101 tcattggttc atacagtaga attagttaaa ggtgagaaat ataatgtgtg agattagtat
     5161 tattgttcct gtgtataaag ttgaaaaata tttaagaaaa tgtgtagatt cgattttagc
     5221 ccaaacattt actgattttg aagttatatt agtagatgat ggatctcctg acaatagcgg
     5281 gaaaatctgt gatgagtatg ctgaaaaaga taatcgagta cgtgtgatcc ataaagaaaa
     5341 cggcggatta agcagtgcta gaaatgcggg gattgatgtt gcgagaggca aatacttagg
     5401 ctttgttgac agtgatgatt atattgatga agatatgtat gaaattttgt atgagaattt
     5461 gaaaattcat gatgccgaca ttagctctgt tgaattgatt cctttttatg gtgatagata
     5521 taagaaagct aataaagaaa agaaagtaat tatattaaat aaaaaggaag caataaaatc
     5581 tgttttagaa ggaacgcaat tttatgcata cgcatggaat aaactttatc gtaaggaact
     5641 ttttaaagat aatcgttact tggatggaaa aacatttgag gatgcatata taataataga
     5701 tctattattt caaacagaaa aaattgttgt ttccaatgaa gagaaatatt tctatcttca
     5761 aagaaatgat agtattatgg gaaaaagttt ttctatgaat aatttcgatg ttatagaagc
     5821 atggcaatat aacaaagaga agattttaga tgcttttcca gatttgcatg attcctatta
     5881 tcgccgacta tgttgggcat atttttacgt gttggataaa atggttttat caagtagtta
     5941 tcaacaaatt ccacgtaaaa aaacagtaat tgaattttta aagaagaaca gaaattttat
     6001 attaacttat aagggattca ctaaatcgcg caaaatagct attattgctc tgagctttag
     6061 agtagattgg tataggaaat tggttgaaca aaaaaataaa aaaaattaga atgtttaaat
     6121 tagtaaataa aagaaaagga attgaaaact aaaatgtgca gaataagtat aatagttcca
     6181 gtttataatg ttgaaaaata tttaaaaaag tgtgtcgatt caattctagg ccaaacattt
     6241 accgattttg aattattatt aattgatgat ggttctacag ataattcagg aagtatatgc
     6301 gatgaacttg cgaaaacaga caaccgaata aaagtgatac ataaggaaaa tgggggtcta
     6361 agtgatgcca gaaatattgg tattgaggta gcaaagggag attttattgg gtttatagat
     6421 agtgatgatt acattgatga agatatgtat gcatttttat ataacaatat attgaaatat
     6481 gatgcagagt tatcaatgtg tggtatttat gacgtccata agaataaaga aattaaaaag
     6541 ttaactcctt tttctcaatt ggttactaaa tctgaagcaa ttgaattagt attagatggt
     6601 aagctagttg ttgctaatgc agtaagcaag ctatataaaa aagaattatt tgagaatgtg
     6661 agatatccaa aaggaaaaat tgctgaagac gcagcagtaa tactgaaaat tatcaatcag
     6721 tgtaataaaa tccatgttga tacttctcaa aaatattatt attatcatcg tggagatagt
     6781 attactagta aaaaatttac aaaaaaagat tttgatataa ttgatgtatg gaatgagaat
     6841 gaaaa
//
LOCUS       DACEKH010000114         6771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEKH010000114 DACEKH010000000
VERSION     DACEKH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.118"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            68..151
                     /locus_tag="IW662_002408"
     CDS             68..151
                     /locus_tag="IW662_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ0867209.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            276..1013
                     /gene="erm(B)"
                     /locus_tag="IW662_002409"
     CDS             276..1013
                     /gene="erm(B)"
                     /locus_tag="IW662_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0867210.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1183..1443
                     /locus_tag="IW662_002410"
                     /pseudo
     CDS             1183..1443
                     /locus_tag="IW662_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1606..2271
                     /locus_tag="IW662_002411"
                     /pseudo
     CDS             1606..2271
                     /locus_tag="IW662_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2307..2552
                     /locus_tag="IW662_002412"
     CDS             2307..2552
                     /locus_tag="IW662_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0867211.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2655..3524
                     /locus_tag="IW662_002413"
     CDS             2655..3524
                     /locus_tag="IW662_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0867212.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3505..4239
                     /locus_tag="IW662_002414"
     CDS             3505..4239
                     /locus_tag="IW662_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0867213.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4272..5184
                     /gene="ant(6)"
                     /locus_tag="IW662_002415"
                     /pseudo
     CDS             4272..5184
                     /gene="ant(6)"
                     /locus_tag="IW662_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            complement(5293..6480)
                     /locus_tag="IW662_002416"
     CDS             complement(5293..6480)
                     /locus_tag="IW662_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0867214.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT         2352 a         1074 c         1326 g         2019 t
ORIGIN      
        1 tgtgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg
       61 attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca
      121 gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact
      181 aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat
      241 ttatttacag atgtaataac ttcaggagtg attacatgaa caaaaatata aaatattctc
      301 aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag
      361 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
      421 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
      481 aaaaattaaa actgaacatt cgtgtcactt taattcacca agatattcta cagtttcaat
      541 tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa
      601 ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag
      661 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
      721 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
      781 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
      841 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
      901 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg
      961 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
     1021 agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta
     1081 gatatactac tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg
     1141 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
     1201 aagtgtggca acaatattgt attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa
     1261 tgtaagttta ctttagctga acattttaga aagaaaaaac taaccaaaac gaatgtaaaa
     1321 gaattactgg agggaaaaga aaccctggta aaaggaatca aaaacaaaga gaaaaagccc
     1381 tacaatgccg ttgtaaaaat tggggaaaag ggatatattg attttatctc tttttcaaaa
     1441 taaacatggg gctgacccaa aagtagctcc tatatgaaaa atagaggtct ttccatttag
     1501 atagtagtgg aaagacctct atttatcttt tgaaagtgaa tacaaaaata aagcacactc
     1561 tgctataata taggtgatta atccatttga gagaggtgct ttatcatgta tcaaaattat
     1621 accataaacc aactttgttt gcctatagat cttgaaatta aattagaaga aaatgatttt
     1681 gctcatgcca ttgtgcagtt tgttgactct attcccgatg aagtctttct tccttattat
     1741 caatctatgg gtagacccca atatcatcct cgtatgctac tttccattat tctttgtgcc
     1801 tatattcaag gggtttattc gggccgccaa atacaaaata tgctgataga tagtattcgg
     1861 atgcgctact tatctcaaga gcaattccct aactttcgta ctattaatcg ttttcgcgtt
     1921 catccgatta tgaacgatat tctagatcat tcatttgttc aatttcgtga gctactggtt
     1981 caaagtggct taatcagtgg cagtgctcta tacattgatg gtacaaaaat tgaagcggat
     2041 gcgaataaat actctttcgt ttggaaaaaa agtattttaa agtataagga tagccttgat
     2101 caaaaagccc ttgaaaacta ccaacaaatg gtggaaacaa aaatcttacc tgagttaata
     2161 gatgatttaa aggatgaact ttctatagaa gagattgaaa ggattcgtca atctttggaa
     2221 gaaaaggaaa atcaattaat tgaagcgatt gaccaaaccg aaacagtaga agggggctga
     2281 cccattatta ataaggagtg gacaacatga ttgatgtaaa tgagctattg gaagaagcta
     2341 ttagagaaac tgaaaatcta aatgatggcg aagtttttct tgttaaagat ttattcaagg
     2401 gatatgtggg gaataggata cccagaaagg atcgacttct gcttggaacg ttatttttaa
     2461 accatgttag caaaatgaat ggtaatttaa aggctattga aaagacttca tctaatcagc
     2521 agaggtataa aaagacaatt gataagtttt aactatcttt tgtgaaatta aaaaaattat
     2581 aaatatgttg agtagaagca aaagatttgg cttggaattt ttacagcagt gaatcgataa
     2641 aaggagcatg cttgatggta gataatatta ttaaatcagt agcagagaaa ttatcctctc
     2701 tgtcttatat agaaggtatt gttttaggtg gttcacgtgc aaggggcacc catacagagg
     2761 attcggatat agatatcggc atctattaca attcagaatc atttgacata aatactatta
     2821 atcaattcgc tacaaagctg gatgatgagc atagaaataa ccttgttgta cctcccggag
     2881 catggggtga ttggattaat ggcggcggat ggttagtcat aaacgggtat catgtggatt
     2941 taattttacg tgatattaaa cgtgtggaac aaataatgaa agatacagag cacggaattg
     3001 ttactgccaa ttatcagact gggcatcccc atggttatat tagtgcaatg tatcgaggag
     3061 aattagcgat tagcaaaata ctatatgcta agaatgaaag cttatgcgaa ttaaaaaaac
     3121 aggcagaaac ttatcccaat gctttgcaga aaagtttagt taactttttt atgtttgaag
     3181 cagggttctc tttaatgttt gtaaaagcaa attcgggaac agacgataaa tattatattg
     3241 cgggtcatgt ttttcgtata gtttcatgtt taaatcaagt gttatttgca tgtaataatg
     3301 cttattgtat caacgaaaag aaagctataa aactgcttga aacttttgaa cataaacctg
     3361 aaaaatatac cgagaaggta aatcatattt ttgaagtact cggtatctca ctttttgaat
     3421 gctacgacat gaccgagaag ctttataatg aagtgaatga aattgtatcg gagataaata
     3481 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     3541 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     3601 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3661 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     3721 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     3781 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     3841 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     3901 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     3961 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     4021 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     4081 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     4141 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     4201 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     4261 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     4321 aggatgaacg aacgtattcg aattgtgacc cttgaggggt cacgcgcaaa tattaatata
     4381 cctaaagatg aatttcagga ttatgatatt acatattttg taagtgatat agaaccgttt
     4441 atatctaatg atgactggct taatcaattt gggaatataa taatgatgca aaagccggag
     4501 gatatggaat tattcccacc tgaagaaaag ggattttcct atcttatgct atttgatgat
     4561 tacaataaaa ttgatcttac cttattgccc ttggaagagt tagataatta cctaaagggc
     4621 gataaattaa taaaggttct aattgataaa gattgtagaa ttaaaaggga catagttccg
     4681 actgatatag attatcatgt aagaaagcca agcgcaaggg agtatgatga ttgctgcaat
     4741 gaattttgga atgtaacacc ttatgttatt aaaggattgt gccgtaagga aattttattt
     4801 gctattgatc attttaatca gattgttcgc catgagctgc tgagaatgat atcatggaag
     4861 gtcggcatcg aaacaggctt taaattaagt gtaggcaaga actataagtt tattgaaagg
     4921 tatatatccg aggatttgtg ggagaaactt ttgtccacct accggatgga ttcctatgaa
     4981 aacatatggg aagcattatt tctatgccat caattgttca gggcggtatc cggtgaggtg
     5041 gcggaaaggc ttcattatgc ctatccggag tatgatagga atataacaaa atataccagg
     5101 gacatgtata aaaaatacac tggtaaaacc ggctgcctgg atagcacata tgccgctgat
     5161 atagaagaga gactaactat atagagtcaa gtaaaaatta gccaaatcaa aaaatatttt
     5221 ataacagata attccgaaaa aagggtacaa caaataagct tcttaccatt agtttttttg
     5281 aatgttcaat agctaatcag aaacaattat ataaggctcg tttttagtca aaatagcatg
     5341 aatcacgtaa caaagcttac gagaaaccgc acctaaggca gttagatgat gttttccttc
     5401 ggaaactttt ttctggtaaa acgcactaaa aacaggatca cattgagaag ctactaaagc
     5461 cgccgaaaac aaggcttttc gcaaatatgg agaccctcgt ttactcataa cattatgcgt
     5521 tgcctcatat tgccctgatt gtgtcacgga agcgtctatc ccagcgtaag caacgagttt
     5581 tttcgggtta gaaaattttt gaatatctcc gatctcacca agaatagtcg caccgtttat
     5641 aggaccaatt ccaggaattg tgagaataac agaatttaaa gtgtccatta tgtgcttgat
     5701 ttctctttcc gtttccttta tttggctttc aatgaatttt aattgttcaa tcattgaacg
     5761 tagttggaag gtgaacgctt cttgagcaaa ttttactcca aaggagttag aagctgcttg
     5821 cttcaattgg tccgcttttt ctgccttgat tcggttcctg cttaactcag ctaagtattg
     5881 ggccaaagtt tctgaggaaa tgttttctaa gtcgatcggg gcactaaacg ccagcaataa
     5941 ctctttagaa gcttttccga aaacgccctg cttggtaaaa atcgtcgcgt attctggaaa
     6001 gacttgatcc aatacggcta taatttttct tttgaagtcg ctcgctgtac ctactaaata
     6061 agttcggtaa cgagaaagtt gtttcaaaga aaaaacattt tcatcggata agaccgtttc
     6121 gacaaatgag ccatagcgca ttaagtcagc aatcaaaaca gaatcaataa tatcgttttt
     6181 tcgtttccgt atttctgtac ctttacgcca tccgtctgtt tgaaggggat tcacaacgtg
     6241 aatcaaataa tctgattcgt gaaggtatga aaagatggac agccaatagt gccccgtggc
     6301 ttccatccct ataacaaagt cactgggtgc tccggaatag tcgaccattc gctcaattaa
     6361 tgactctgct ccttcagtgg tgttaggaaa tgaaaaacct tttagtaata cctttccttc
     6421 ctcgttcatg atagaagcaa cgtgagtgcg tttaccgata tcaatgccta aaaagtacat
     6481 gtatatctga cctttcaaaa aaagaatttt ggattgaaat ccactcaact gaaaagcgtc
     6541 actaccttgt acgagatacg gagtaaagta aggactttca acatctaact catacgcaat
     6601 ccacttatca gagagaggag ccagtctctc gagtgcgggt tcaatggccc aaggggggaa
     6661 cggcttagcc tctcaatcca ataaatatat tatctcaaaa gaaatgagtt aataccaaat
     6721 taaaataacg atggttcata gggttatcca atcaaaaccg gaaggttaaa a
//
LOCUS       DACEKH010000115         6701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEKH010000115 DACEKH010000000
VERSION     DACEKH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.119"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1023
                     /locus_tag="IW662_002417"
     CDS             <1..1023
                     /locus_tag="IW662_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0867215.1"
                     /translation="ELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQGTIV
                     DFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQDAW
                     LVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDP
                     FTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVS
                     AHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKD
                     VNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1034..3043
                     /gene="topB"
                     /locus_tag="IW662_002418"
     CDS             1034..3043
                     /gene="topB"
                     /locus_tag="IW662_002418"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0867216.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            3389..3985
                     /locus_tag="IW662_002419"
     CDS             3389..3985
                     /locus_tag="IW662_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0867217.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            3998..4897
                     /locus_tag="IW662_002420"
     CDS             3998..4897
                     /locus_tag="IW662_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0867218.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            4900..5031
                     /gene="ssb"
                     /locus_tag="IW662_002421"
     CDS             4900..5031
                     /gene="ssb"
                     /locus_tag="IW662_002421"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0867219.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            5053..5385
                     /gene="ssb"
                     /locus_tag="IW662_002422"
     CDS             5053..5385
                     /gene="ssb"
                     /locus_tag="IW662_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0867220.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            5430..5663
                     /locus_tag="IW662_002423"
     CDS             5430..5663
                     /locus_tag="IW662_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867221.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(5822..5944)
                     /locus_tag="IW662_002424"
     CDS             complement(5822..5944)
                     /locus_tag="IW662_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ0867222.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6134..6358
                     /locus_tag="IW662_002425"
     CDS             6134..6358
                     /locus_tag="IW662_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867223.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
BASE COUNT         2394 a         1187 c         1418 g         1702 t
ORIGIN      
        1 gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg tttagatcat
       61 gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc aagttatcaa
      121 ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca cattgacaag
      181 aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca tttaaaattc
      241 tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa actacaagat
      301 gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta cgttgaagag
      361 tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat atgtgtggac
      421 aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat cgttgatcct
      481 tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca agtacctaaa
      541 gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc tgaagcaatt
      601 atggccatac acaaaactga aactaattta cagctgacaa atcaattggt gtctgctcat
      661 gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga aacaattgat
      721 ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg cgcagatgga
      781 acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt taatgcagga
      841 atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca tgaacatgaa
      901 tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat ccagcaacaa
      961 gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
     1021 tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag cgaaggctta
     1081 tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc aagatcgatt
     1141 gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt tagattcgcc
     1201 cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta ttttccccaa
     1261 tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg tcaaacgaca
     1321 gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag gggagctgat
     1381 tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta aaagactttg
     1441 gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac gagacgcaga
     1501 agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt ggttaattgg
     1561 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagttt
     1621 ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc aggaaaagat
     1681 tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg ctcaaaatgg
     1741 cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag catttgaagc
     1801 ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc ttcaagtcga
     1861 aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc ttcagtctaa
     1921 aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta
     1981 tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg aaggggaata
     2041 tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg aagcgattcc
     2101 gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc aggaacatta
     2161 cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac taagtccact
     2221 gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg cggaaaagta
     2281 tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagttaa aggcaatcga
     2341 aaaggtgccg ctagatctag gttggaagaa gttatttgga aaagagagcg acggaaaaga
     2401 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtggatct
     2461 acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     2521 tgcaatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     2581 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     2641 ggaatatatc aaggtagaga aaaataagct cgtggtgacg aataaaggga ttttgctttg
     2701 ccaagccgtg gaaaaagagc cgctgcttac gagcgccgaa atgaccgcca aatgggaaag
     2761 ctacttgtta aagatcggtg aacgaaaagg aacacaaacc acttttctag ccaatatcca
     2821 aaaatttgtc tctcacttat tggaagtcgt accagggcaa atccaatcca ctgattttgg
     2881 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     2941 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     3001 gtctgaagcc tgtgatttta aaaggtcaga agggttcatt tgaagcaacg attgttttaa
     3061 aagaagactt cactacaggc tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa
     3121 gaacacgccg cactgcaaaa taatggaggg ttaatcttat gcgtaatacc acacacaaac
     3181 aacaagaaat cgaaaatcaa aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca
     3241 gcaaacgaag actgcagcaa ttgaagaacc agcagaggat tcaaaaagaa atcgaattag
     3301 atcgataaaa tcttcctaac tattgtatac gtataaactt atgttatact gtttttataa
     3361 gtttatacgt atacaaagga gggagaaaat gacaaaagca atggtataca agctgctaga
     3421 agaatttaat ggaactttgg ctttgaagga tgccaaaaaa gcgggcattt ctcctgtaac
     3481 gatcaaacgt atggtagatc gaggggagct ggatcgagag tatccaggtt ttttcacctt
     3541 acctggtcaa tttcctgatg aattgtttat ggcgcaaaaa aaatatgaac gcggcattat
     3601 ttcacatatt accgcgttag atttgtatga tttaacagat atgatcccta gacagatcga
     3661 tctgactgta ccttacggtt atcacgtttc tgaaaaagga ttgaaggaat tcgcagttga
     3721 gctacactat acaaaagcgg aatggtatga attaggtaag attgaaataa aaagtcgcta
     3781 tgggaatcct attatagctt atgatccaga aaaaaccttg tgtgacattt ggaatccctg
     3841 gtataacgtt gaggatgaaa tcaaagtaaa agcaatcaaa aactacatgg agtcggacag
     3901 aaaaaatctt agaaaattga atgagtatcg acgaattttg cctacagata aaacgatgcg
     3961 ctcttatatt atggctttaa attaaaggag aatacacatg acacctacgc aattaaaagc
     4021 aaaagtgaag aatatttcta gagaaaaaga agttgatccg cagcttgttt taagacattt
     4081 tatgatggaa aaattcttgg aaaaaatctc agagtctcct tatcgtgaaa attttgtgtt
     4141 aaaaggcggc ttcttaattg gctcaaagta tggcattgaa aatcgaacaa ctaaagacat
     4201 tgatacgact ttacgggaaa tgaaagtaac caaagaaaca ttgacaacgg ttctaaacga
     4261 tattttttca acgccgacga aagaaggcat tcaatttgag attcaaggaa tgaaagaaac
     4321 gagggaagct gattattatc cagggtttag cttacgtgta ctggcacatt tggaaaacat
     4381 gcggccagat tttaaggtag atgttactac aggtgactcg atctatcctg cgacgatcac
     4441 tcacagtcat aaattgatgt ttgaggatcg aacgattcct ttggaatcct atcctacaga
     4501 acaaattatt gctgaaaaat taagtgcaac ctttgatttt ttaacagata attcgagagg
     4561 aaaagatttt tatgatctct atactattcc taaaatggag aagatcgatg agaagacctt
     4621 gaaagatagt gtgagaaata catttacacg tcgaggaaaa actgatccgt taaagatata
     4681 ctatgaaaag gatatggatg tactgaaaaa tagtcacgag ctaaaagata cctggaagaa
     4741 atatcaaaaa agcaatcctt atgctgcgtc gatcacgtac gatgagacaa tggattcgat
     4801 tagtaatttg atggaaaaag tgattcacgt cgaagaaaaa gagcgaaaac tagcttatca
     4861 aagacaccaa cagatgaata gagaaatgga gagataaaga tgattaataa tgtgacatta
     4921 gtaggacgct taaccaaaga tcctgattta cgttataccg caagtggtac tgcagtagcg
     4981 acttttaccc tagccgtcaa ccgaaatttc acgaatcaaa acggcaattg agaagccgat
     5041 tttattaact gcgtgatttg gcgcaaacca gcagaaacaa tggcgacatt agctaaaaaa
     5101 ggtagcttga ttggtgtagt tgggcggatt caaacgcgga cttacgacaa ccagcaagga
     5161 caacgtgtat atgtgactga agttgtagca gataactttc aactattaga aagtaaagca
     5221 gcgacagaga gccgtgcgca cgctgatcaa tcgtccacca gtccaagcac cgccaccttt
     5281 gaacaacgga atacagcgac gcctaacaac aatggattga acgcttcaca gaatccattt
     5341 ggtggacaat caatcgatat tagcgatgat gatctaccgt tttagtttga gaaagaagga
     5401 gtaagcatgg caaaaaaatt agtctgacga tggatctatt gatacccgta attgcattta
     5461 tagacgggat ttctttgggc gtcactgaaa atcctttggt aaatagaatt gcgaaaggat
     5521 cactatttct tgtattcgtg tatcttgtct gttcttatct tttaaaaaga gcaggaaagg
     5581 ataattcctt ttggcgattg actaaagtca cagtagcagc gacagctgtt atacttacgg
     5641 ctattctacc gctttatatg tagagttaac gggaaatcgt tgaacttctt gcgaaatatt
     5701 gctataatga acatgaaaaa gaggagttgc gctaacaacc cctcattaac agcaccgatt
     5761 aaagaacggt ggcagaatat tagttatttg gaaataaccg ctgacctgct aaagtagacg
     5821 gttatttctt tttgtcattt ttaagcatat ttacaatcaa actaatcaaa gcaatggtaa
     5881 acataccaaa accaagaatg gtttgaattg tctcatatgc ggacaaaagg cttactcctt
     5941 tcatagattt tagcacttac attcataagc accaccacct ttccagaagt cgccaccgtc
     6001 ttcaaacttt actgtcttga ctattatagc atatctaaaa tcgaataaaa attctcaatt
     6061 ttagctgttt ttgctataat tttagcgagg tgatcgtgat ggaacaatat attggtttac
     6121 tcgataaatt agggtgatca agacacagcc attgcttgtg cgatttacat tacgcacgag
     6181 tcatgaacca atcaattgtg tagtggccaa tatagaaata gcggataaat tgctgatttt
     6241 ggaagaaggc aagtatacta tttcagtgac aggtcacttc aataaacgaa agcaactagt
     6301 cattgaatca atgcaaatta gaaaccctga tcattttacg cgatcgatga gcatataaaa
     6361 gaagagctgc agtcctaaat gaggattgta gctctttttg ttttacatag aaacagtaaa
     6421 aatgaaaagg cctgtttagg aaggcacaag cgccttctca attgaaagac ctttctctca
     6481 ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gctacgcgtt cactaagata
     6541 aagggtcttg catagcaaga caaaatagaa aaaatttcct ctcaccaaaa ggttcgctcc
     6601 tgttcgttat ctctttattg atttgtcaga ccggcaacaa gttgcctttt atcaccaaaa
     6661 agcgacaagg acaagggtaa atggcacgct gcgcgccctt g
//
LOCUS       DACEKH010000116         6671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEKH010000116 DACEKH010000000
VERSION     DACEKH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.120"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1014
                     /locus_tag="IW662_002426"
     CDS             <1..1014
                     /locus_tag="IW662_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0867224.1"
                     /translation="ELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQ
                     ERE"
     gene            1025..3229
                     /gene="topB"
                     /locus_tag="IW662_002427"
     CDS             1025..3229
                     /gene="topB"
                     /locus_tag="IW662_002427"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0867225.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            3470..4063
                     /locus_tag="IW662_002428"
     CDS             3470..4063
                     /locus_tag="IW662_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ0867226.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            4076..4975
                     /locus_tag="IW662_002429"
     CDS             4076..4975
                     /locus_tag="IW662_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0867227.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            4978..5451
                     /gene="ssb"
                     /locus_tag="IW662_002430"
     CDS             4978..5451
                     /gene="ssb"
                     /locus_tag="IW662_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0867228.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            5817..6077
                     /locus_tag="IW662_002431"
     CDS             5817..6077
                     /locus_tag="IW662_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867229.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            6522..>6671
                     /locus_tag="IW662_002432"
     CDS             6522..>6671
                     /locus_tag="IW662_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0867230.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKK"
BASE COUNT         2450 a         1163 c         1374 g         1684 t
ORIGIN      
        1 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
       61 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
      121 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
      181 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
      241 tttgagagta gtattgattt acttagctat gctgcactaa atcgtgatca gttaaacgat
      301 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
      361 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
      421 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
      481 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
      541 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
      601 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
      661 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
      721 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg cgtggatggt
      781 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgctca gattcttttc
      841 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
      901 gaagtaaaaa aggattggaa tgatcaattg aagcatgaaa tctaccaaca agagatcaga
      961 aagcaaaaac gtgccatgtt gtttcaacaa ggtagacagc aagaaaggga gtgaaatcgt
     1021 catgatgaaa accgttattt tagcagaaaa accgtcacaa gcgaaggctt atgccgatag
     1081 cttttctaaa gctacccgaa aagatgggta ttttgagatc caagatcgat tgttttcagg
     1141 agaaactgtg atcacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     1201 cgatgaaaat tggaaacaat ggtcattaga gcatttgcct attttcccta cacattatca
     1261 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     1321 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tagcttggac
     1381 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     1441 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     1501 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     1561 tagcccgctg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     1621 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     1681 ccaaaaagaa ccatattttg aaggaaaagc ccaagtaatc gctcaaaatg gcgcatttga
     1741 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     1801 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     1861 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
     1921 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaagggaa
     1981 atatctaagc tacccacgaa cagatacccc ttatattaca gaaggggagt atgcgtatct
     2041 gttggatcac ttagacgaat acaaacactt tttaaaggcc gaagccattc ctactcctat
     2101 tcatacaccg aatagtcgat atgtgaataa taagaaagtc caggaacatt acgcgatcat
     2161 tcctacaaag acggtcatga cggcagcggc ttttgaacaa ttaagtccac tgcagcaggc
     2221 tatttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga
     2281 agaaacaacg atcttgacgc aagttcagca gcttcagcta aagacagtcg gaaaagtacc
     2341 agtagatcta ggttggaaaa agctgtttgg caaagagagc gacagaaaag aaaaggaaga
     2401 agaaccgcta cttcctaaag tgacaaaagg agaagcggtg acggtagatc tacaagtcct
     2461 tgaaaaagaa acgaagccac cacaacctta taccgaaggt acgttgatca cagcgatgaa
     2521 aacagctgga aaaaccgtgg atagcgaaga agcacaatcc atattgaaag aagtagaagg
     2581 aatcggtaca gaagcaacac gagcaaatat tattgaaact ttgaaacaaa aggaatatat
     2641 caaggtagaa aaaaataagc ttgtggtgac aaataaaggg attttacttt gccaagccgt
     2701 ggaaaaagag ccgctgctaa caagtgccga aatgaccgcc aaatgggaaa gttacttatt
     2761 aaagattggt gagcagaaag gaacacaagc tactttctta gctaatatcc aaaaatttgt
     2821 ctctcacttg ttggaagtcg taccgggaca aatccagtca acagattttg gatcaacgtt
     2881 acaagaggtg aaagcagcca gtgaaaaaca ggaagcaacg cgtcatttag gtgtttgtcc
     2941 aaaatgtcgg gaacaagaag tcttgcttta tcaaaatgtg gcggcatgta cgtctgaagc
     3001 ctgtgatttt aaattatgga caacgattgc caaaaagaaa ctcacagcca cacaactgaa
     3061 agagatcatt caaaacggcc gaacaagcca atcagtcaga ggactaaaag gtcagaaagg
     3121 ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca acaggctttg aattttctga
     3181 aaagaaaaaa acgaactata agaaaagaac acgccgcact acaaaataat gaaggggtta
     3241 tcctatgcga aatacaacac acaaacaaca agaaatcgaa aatcaaaaag caaaaattcg
     3301 tttagaacaa gagaaattac gtgcaaataa aagaagactg caacaattaa agaaccagca
     3361 gcgcattcaa aaagaaatcg aattagatcg atagacgtta atcttgttac aaataaacta
     3421 ctatgatata tttgatgtag tgtatttgta acgaagggag gggaagtaaa tgcaagataa
     3481 actgaaagaa ttgttggcta ttcgcgatgg aaatttatct atgaaggaag ccaggaaatt
     3541 tggaatcgca gcaaccactg tacaaagatt ggtgaatcaa ggaaaattga tcaaagttga
     3601 tcgtggttat tatgtagagg atggtcatgg aatagatgat ttattttggc ttcagcaacg
     3661 attttccagg ggaatcttct ctcatgaaac gactttagat atttaccagc tttctacgaa
     3721 tatgccgaaa ttcattcact taacttttcc ccatggctat aacgttgatc gttcaattac
     3781 aaaagagcag cagttacaat ttcatttcgt gaagaaagaa gtttatgaac taggaaaagt
     3841 agagggaact tcctttcaag gaaatcctat tactatgtat gataaagagc gtacactttg
     3901 tgatatttgg aatccgagat atgaatttga atatgaaatg aaattagaag cattaaaaga
     3961 gtacatggaa gacccattga gagatccaag taaattaaga gattacatga caaaactaca
     4021 tgtagaaaaa gaaatgaaat accacatgca aagcttatat tgatggagga aaaagatgac
     4081 accgacacaa tttgcagcaa aaacaagaaa tttagcaaag agtacaggat taaatgcaca
     4141 gttagtgcaa cggcatttta tgatggatcg tctaattgaa caaatttctg aatcaaagta
     4201 caaaaatgac ttcattttga aaggcggctt tttattagga tcaaaatatg gaatcgatag
     4261 aaggacaaca attgatatcg atacgacatt tagaaatagc aaattaactg aagaaaaatt
     4321 gaagagcatt tttaatgatt taactagaaa acccacaaaa gaaggcattg ttttctctat
     4381 tcaaggaatg aaggaaacaa gagaagccga tttttatcct ggatttcaaa ttaaattggt
     4441 ggctaattta gagaaagtac gtgtaccgtt taaaatggac gtgacaacag gagattctat
     4501 ttatcctgaa gtagatacac actatcacca attaatgttt gaagataaaa aagtggagat
     4561 tccagcttat ccaacagaac aaattattgc agaaaagcta agtgctacgt tcagctttgg
     4621 cacagataat tcgagagcaa aagattacta cgacttattt accattccga aactagaaaa
     4681 tatcgatacg aaagagttat acaaaagtgt tcgaaataca tttacaaaac gtggggatac
     4741 aaaaccgttg tccttttatt atgaaagaga aatggataag atcagaggaa atgactattt
     4801 ggaaaagcag tgggaaaaat atcgctcagt taacacgttt gcgaattcca tttcatttac
     4861 agatacagtc aacgaaattg atcacctaat gaaaagtgtg atcaaaattg aaaatcaaga
     4921 aagaaaacgg cagtttaaaa gatcacaaca aatgaatcaa gaaatggaga gataaagatg
     4981 attaataatg tgacattagt aggacgctta accaaagatc ccgatttacg ttatactgca
     5041 agtggcacag cagtagcgac ttttacccta gccgtcaacc gaaatttcgc gaatcaaaac
     5101 ggcaatcgag aagccgattt tattaactgc gtgatttggc gcaaaccagc agaaacaatg
     5161 gcgacattag ctaaaaaagg cggcttgatc ggtgttgtcg gacggattca aacacgaact
     5221 tatgacaacc agcaaggaca acgtgtgtat gtgactgaag ttgtagcaga taactttcaa
     5281 ctattagaaa gtaaagcagc gacagagagc cgtgcgcacg ctgatcaatc aaccaccagt
     5341 ccaagcccca ctacctttga acaacgggat acaaacaatg gattggacgc ttcacaaaat
     5401 ccatttggtg gacaatcaat cgatattagc gatgatgatc taccattcta agtagcattt
     5461 tctattggtt gcatttgcta cggtttatgt tataatttag tcaacaaaaa gaattttcaa
     5521 tctataacca aaaaccacta gctggcgggc tagtggtttt cttttacgtt tctattataa
     5581 gaattaacgg gtaggcttac tgtctttctt ggcaaattta caatcaagcc atttgcagat
     5641 atagtgcgcg gctacttctg ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc
     5701 tataacctcc tccccttgcc agattaggcc ggggtagcag acagcaagac tattatagca
     5761 taattgaaat attcaaatga ttggattttt gctataattt tagcgaggtg attgtgatga
     5821 acaaatatgt tggtttgctt gataaaatcc gagtaataaa aacgcaaccg ttattggttc
     5881 gctttacttt acaaacgatt catgaatcga ttaattgtgt agttgcagat attgaaatta
     5941 tcgataaatt gctaattatg gatgatggaa aatacaatat tgccgtcaca ggtcacttca
     6001 ataaacgaaa ccagctagtt attgctagca tgtatgttag aaatcccgat cagtttacgc
     6061 gattgatggg catataaaaa aagaagagtt gcaatcctaa tgtaggactg cagctctttt
     6121 tgttttacat agaaacaaga aaaatgaaaa ggccttttta ggaaggcgcg agcgccttct
     6181 caattgaaaa acctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac
     6241 gttgcacgtt cactaagata aagggtcttg caaagcaaga caaaatagaa aaaacttcct
     6301 ctcaccaaaa ggttcgttcc tgttcgttat ctctttattg atttgccaga ccggcaacaa
     6361 gttgcctttt atcaccaaaa agcgacaagg acaagggtaa atggcacgct gcgcgccctt
     6421 gtccttgtcg cttttcgttg attcattctg gccaacaaat caataaagaa agaactaacg
     6481 aaaggaagta aaacctttcc taagaggagg cagaatcaat catgcgaaag aaatttgatt
     6541 attggggtgt tcccttcagc gaattattcc caaactatca tgcaccacat actgtcgaat
     6601 gcgattgtgg agaaagagcg aaatgtatca aaagctatcg tttgtatcaa tgtccaacat
     6661 gcgggaaaaa a
//
LOCUS       DACEKH010000117         6610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEKH010000117 DACEKH010000000
VERSION     DACEKH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.121"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            244..2475
                     /gene="pflB"
                     /locus_tag="IW662_002433"
     CDS             244..2475
                     /gene="pflB"
                     /locus_tag="IW662_002433"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ0867231.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            2719..3480
                     /gene="pflA"
                     /locus_tag="IW662_002434"
     CDS             2719..3480
                     /gene="pflA"
                     /locus_tag="IW662_002434"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ0867232.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            3565..4491
                     /locus_tag="IW662_002435"
     CDS             3565..4491
                     /locus_tag="IW662_002435"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ0867233.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            4673..5566
                     /locus_tag="IW662_002436"
     CDS             4673..5566
                     /locus_tag="IW662_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ0867234.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            5700..5825
                     /locus_tag="IW662_002437"
     CDS             5700..5825
                     /locus_tag="IW662_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0867235.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5959..6591)
                     /locus_tag="IW662_002438"
     CDS             complement(5959..6591)
                     /locus_tag="IW662_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867236.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         2395 a         1104 c         1301 g         1810 t
ORIGIN      
        1 gtattaatac aggtggcgtc tctttttcat tcatttttaa taaatcctta tacgtttgca
       61 aaaaaagatg aaagtgttca caatgcttaa atgttggtat gtcaacgttg atttaaatat
      121 atgtaaataa aacataaaaa ttgttaaata aaaaacaaga aaacccttgc aaagataggg
      181 tgctgtgtta tactaatcac gtaaaaactg ttacataaaa cagaaattta ggaggtccat
      241 acaatggagc aatggaatgg atttaaaggt accaaatgga aagaaggcgt agacactaga
      301 gactttattc aaaataacta cactgaatat cgcggtgatg acagcttttt ggaaggccca
      361 acagaagcaa cagaaaaatt atgggatgaa ttgcaaaagt taaatgatat ccaattcgag
      421 cgtaacggtg tctatgatat ggacacagaa atcgtatcaa ctattacttc acacgatgca
      481 ggctatttgg ataaagattt ggaaaaaatc gtcggtctac agacagacaa acctttgaaa
      541 caagcattca tgccgttcgg cggaatcaat atggctaaca atgcgctaaa aagcaatggc
      601 tatacaccaa acgatgaatt gacacatatt ttcactgact ggcgcaaaac acataatcaa
      661 ggtgtatttg acgcttatac tgctgaaatg cgtgctgctc gtaaaaacaa aatcattaca
      721 gggcttccag acgcttacgg tcgcggacgt atcatcggtg actatcgtcg tgtggcattg
      781 tatggaatcg actatttgat ggaacaaaag aaaaaagatc atgatatgac tggttatcat
      841 acaatgagtg aagaagtgat tcgtttacgt gaagaaatca ctgaacaata tcgtgcatta
      901 gcgcaaatga aagaaatggc tgcgaaatat ggctatgata tttctaaacc agctgcaaat
      961 gcaaaagaag ctgtccaatg gctatacttc ggatatttag cagcaatcaa atcacaaaat
     1021 ggtgctgcga tgtcaatcgg acgtatctca gcattccttg atatctatat ccaacgtgat
     1081 ttggataatg gagtaatcac agaaaaagaa gcacaagaat tgatcgacca cttagtaatg
     1141 aaattacgta tggtcaaatt cgcgcgtaca cctgaatata accaattatt ctcaggattc
     1201 cctatctggg ctactttatc cattgcaggt atgggattag atggtcgttc attagttaca
     1261 aagaatgatt tccgtctgct tcatacatta acaaatatgg gaccttcacc agaaccaaac
     1321 ttgactgttt tgtattctga acaccttcca gaaggattca gaacatatgc tagcagaatc
     1381 gctatcgaaa gttcttcgat tcaattcgaa aatgataatc agcttcgtga acaatggggt
     1441 tctgatgatt gtgcgattgc ttgctgtgta tcaggaacag ttgtcggaaa agacatgcaa
     1501 ttctttggcg ctcgtgctaa cttggctaaa acactattat atgccatcaa tggcggagtc
     1561 gatgaagtga ctggcgccca agttggacca gaatatcgtc caatcacatc agaaatacta
     1621 gactttgaag aagtgaaaca taacttcatg aatatgatgg attggttagc agaactatat
     1681 gtcaatacgt tgaacatcat tcattatatg catgataaat attcttatga agcagctcag
     1741 ctggctttga tggattctga cttgcaacgc acatttgcta caggtattgc tgggatctct
     1801 catgcggcag acagcttgtc agcgatcaaa tatgcaaaag taaaagcaat ccgtaatgaa
     1861 aaaggaatag cagttgattt tgaagtggat ggcgacttcc ctaaatacgg aaatgatgat
     1921 gaccgtgcag atgaattagt ttcatggatc ttggaatact tcatgggaca aatcaaacgt
     1981 cataaaactt accgcaactc aaaaccaaca acttcattat tgacgattac ttcaaatgtt
     2041 gtttacggta aaaatacagg aaatactcct gatggccgtg aagctggaaa accacttgcg
     2101 ccaggagcaa atccaagtta caatgcggaa caaagtgggt tattagctag cctgaactca
     2161 acagctaaaa tgaattacgg ctgtgcattg gatggaattt caaataccca aacaatcaat
     2221 ccttcaggtc ttggaaaaga acaagataca agaatcgaaa acttgcgtaa tgtcttagat
     2281 ggatacttct caaaaggagc ttatcatttg aatgtcaatg tattcagtaa tgatttattg
     2341 cgtgatgcaa tggaaaatcc aatgaaatat ccgaacttga cgatccgggt atctggttat
     2401 gcagttaaat tccgtgactt gacaagagaa caacaattag atgtcttgat gagaacttca
     2461 cacgacagaa tgtaaaaagt tcgtgaaaaa agtttttcga cttagcgtcg aaaggacgtt
     2521 gaatagccat cgcatcaaaa tcgaataatc agtatgctta tttgaaagaa acgatttaca
     2581 ggggcaggac ttgtatgttc cgtccctgta aattttacac ataacgaagc ggcaattgcg
     2641 aaaaattttg ttcagaaagc ttcgcttcga cagctaactt gtacttaaaa tgaaatcaaa
     2701 gaaaagatgt gaacttaaat ggaagaaaaa acaatcggct atgttcattc tatcgaaact
     2761 ttcggttcag tagacggacc tggactgcgc tttgtcgtgt ttatgcaagg atgccgcatg
     2821 cgttgccaat tttgtcacaa tccagatact tggaatattg gcggagggaa agaatacaca
     2881 gcagatgaac ttttagataa agcagaacgc ttcagacctt actggggaga taaaggtggt
     2941 attactgtta gtggtggaga acctctattg cagatcgatt ttttgatcga actatttaaa
     3001 aaagcaaaag agcggaagat gcatactaca ttggatacat gcggaaaacc ttttacgtat
     3061 gaagaacctt tcttttcacg ttttcaagaa ttgatgaagt atacagattt actgttattt
     3121 gacatcaaac acatcgataa tgaagagcat aaaaaactaa cgcattggga caatgaaaat
     3181 attttagaaa tggctcaata tttgtcaaaa atcaacaaac cggtttggat tcgccatgta
     3241 ctagttcctg agcgaagtga ttatgatgaa tatttgatcc gactagacaa ctttattcaa
     3301 accttgtcaa atgtagacag agtagagatt cttccttatc atacgatggg aaaatacaag
     3361 tgggaaacac ttggtttgaa atatccatta gaagggatcg aaccacctac aaaagaacgt
     3421 gtcgaaaatg ctaaacgatt gcttcataca gaagagtata caaaatattt aacgcgatag
     3481 gtcgcatcgg aaagaagata agtattgctt ctttccgttt ttttgctatt attaaatagg
     3541 agaaatcaca aaggagcgaa tgacatgtca aaaattttag tttttggtca ccaaaatcca
     3601 gatacagatg caatcggggc agccattgcg ttttcacact tgcaaaatga attaggtaaa
     3661 gaaacagaag cagttgctct aggtacacct aatgaagaaa cacaatacgc attagaccac
     3721 tttcagctat ctgcaccacg agtagtagag tcagctaaag cagaaaatgc agatgtcatg
     3781 cttgtggatc ataatgaatt ccaacaaagt atttcagata tcagcgaagt aaatatttta
     3841 gctgtagtag accaccatcg tattgcaaac tttgaaacag ctaatccatt gtactatcgt
     3901 gcagaaccag ttggatgtac aagtacgatt attttaaaac tatataaaga aaaccaagta
     3961 acaattccta aagaaatcgc aggaatcatg ctctcagcta tcatttcaga tacattgttg
     4021 ttcaaatcac caacatgtac gcctgaagat gaagcagctg caaaagaact tgcagcaatc
     4081 gctgaagtcg atctaaacac ttatggactt gaaatgttga aagcaggaac aaatctaagt
     4141 gataaatcag ctgcggttct tcttgatttg gatgcaaaaa gtttcccaat gggcagttcc
     4201 aacgttcgta tcgctcaagt caatacagtg gaccttaatg aagtgatgga tcgacaagca
     4261 gagctagaag cagcaatgga agatgaaatg aaagcaaatg gctacgatct gttcgtatta
     4321 gttgtcacaa acatcttaga cagtgattca gaattacttg ttgctggtaa aggaaaagaa
     4381 aaagtagaag aagctttcca aacaactttg accaacaacc gtgctttgct taaaggtgtc
     4441 gtttcacgta aaaaacaagt cgtacctcaa ttgacggctg tatttagcta atcaaatggt
     4501 agtatgaagc attcaaaatg aatggataag cgattgatat aaaaagaaac atagaacata
     4561 ctaaaggccg aagaaatgga ggagaataac agtcaattat gactcttgtt cttcctcctt
     4621 tttacgtaaa agccttttaa gtaaaataaa acggatagaa aagagaacat atatgaacta
     4681 ttattcaaaa aataaggaaa acttttatca aaaactgacc gatgatccgc ttttttcttc
     4741 attgaccgat tatctatatg aacatcgaga acaagaaacg attctaagag aattgaaaaa
     4801 agaattttcg caaaataaat tttcccattt tcttgattta ttgatcgatg caggattaat
     4861 caagagggaa gaacgaagat atcatttgaa ctttccaata tttgatccta aagattactt
     4921 gcaacaagcc acttcggctg ctgaaaccat agctgaacaa ttgaagagac tctctgttga
     4981 tgaacaaaaa ttagcgatgg gagaagttat ttgggcgtat tgtttcgaag atgaacggaa
     5041 agaagcgtat ttttatggag taagaaacag ccgagagaca gaattgctga ggacaactgc
     5101 aggaaatgag aagtaccgtt ttatcacact ttcctctata gaacacttcc cgcttacttt
     5161 ggctaattac ttttttgtac agaagaatca gcttcctgta acgaaggcat tcaaagaatt
     5221 agccgaactt attggagacg taaatgaatc gtactttttt gatcaaatag aagttatcgt
     5281 tgacaggata cgtaagaata aatataaaaa tcgccggcct agtattttcc atcagtctct
     5341 acttgtgacg aatactataa aggaagaaga atcattcaca ttagaattac ctattgtcga
     5401 aaaaaacaat tttgagattg aattaccaac attggatcct tcattgacaa tggaggaaat
     5461 ggcctttctt aaacgacaaa tcttttctga gctgagcaag aaatttatac cgcatgcatt
     5521 ttcttatatc aaagaatatg gaacagtttt agtgagtaaa acctaagaga gcagtaagca
     5581 aaaattatac tgactcttgc aggaggagcc tttctcttcc tttatggtta aaagaattat
     5641 ttgagtattt tgcctatcta ccacctatta gattcactac atggtctgga aacaaaggaa
     5701 tgcaaaaaaa tggtgagaaa tgtggaatga caaaggaagt agtgataaga aaagttagat
     5761 ttttaaataa tcaatattat gattcagtca agtatggcat tttatgggaa gaactagctg
     5821 attagattag gaagtccatt tgataatcca gaatcactaa gaaacgaatg gcgagtcatt
     5881 aataatttat ccaatcttgg gttattcttt tagattgaag tgtttaaaac taaaaaagca
     5941 tttatgaagt cgtaatagtc aggcttcaaa atgcttttta ttttgtttgt tttttctttg
     6001 ccacataaat acacctagac tgacgatgaa gagcatatcc aacagatttc ctgcgacttg
     6061 gatcgtcgaa gggttggtca gtggcattaa agcaaggaca aaaaaggaaa acaagctgta
     6121 aataaaacag gtgacaggaa ctaatcgaaa gaagagcgga catttcaatt ggctaaaaat
     6181 gagtgaaaaa ataaaataag taaaaatgga taaaatcacg actaaaccat tcatgataaa
     6241 gaagaaaagc gtattgtgat taaacgaata aaaaagaatt gctaaaaaag aaaacgtttt
     6301 aaatgcaatc aatacgcttg aagcttcgtt taacgtacca ggatgtcgat gaaaaaaata
     6361 ggcaccgaaa taaacagaaa acagtaagcc gcaaagagaa gacaaaatca aaaaaaacgg
     6421 attttcttcg gcttctgtga tatcagaatg atccaaaagc aaccagccta atgcactaga
     6481 aggcgcatgt gaggtcttaa ggtatgaata acatagaaaa aataagatcg tcaaagttgc
     6541 aaaaaaagag cctaaatgta aaaaattagt ggttctacgt tgaatttcca taatatctcc
     6601 aaattaaatt
//
LOCUS       DACEKH010000118         6467 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEKH010000118 DACEKH010000000
VERSION     DACEKH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.122"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(109..564)
                     /locus_tag="IW662_002439"
     CDS             complement(109..564)
                     /locus_tag="IW662_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867237.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IW662_002440"
     CDS             complement(718..1926)
                     /locus_tag="IW662_002440"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0867238.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IW662_002441"
     CDS             complement(2038..2370)
                     /locus_tag="IW662_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0867239.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IW662_002442"
     CDS             2518..3393
                     /locus_tag="IW662_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0867240.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IW662_002443"
     CDS             complement(3475..4056)
                     /locus_tag="IW662_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0867241.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IW662_002444"
     CDS             complement(4062..5321)
                     /locus_tag="IW662_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0867242.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IW662_002445"
     CDS             complement(5323..6369)
                     /locus_tag="IW662_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867243.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1804 a         1337 c         1102 g         2224 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaa
//
LOCUS       DACEKH010000119         6016 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEKH010000119 DACEKH010000000
VERSION     DACEKH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.123"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(229..714)
                     /locus_tag="IW662_002446"
     CDS             complement(229..714)
                     /locus_tag="IW662_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867244.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(853..1098)
                     /locus_tag="IW662_002447"
     CDS             complement(853..1098)
                     /locus_tag="IW662_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ0867245.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1291..2091
                     /locus_tag="IW662_002448"
     CDS             1291..2091
                     /locus_tag="IW662_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0867246.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2110..3978
                     /locus_tag="IW662_002449"
     CDS             2110..3978
                     /locus_tag="IW662_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0867247.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3971..4462
                     /locus_tag="IW662_002450"
     CDS             3971..4462
                     /locus_tag="IW662_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ0867248.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4450..5004
                     /gene="srlA"
                     /locus_tag="IW662_002451"
     CDS             4450..5004
                     /gene="srlA"
                     /locus_tag="IW662_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ0867249.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5022..>6016
                     /locus_tag="IW662_002452"
     CDS             5022..>6016
                     /locus_tag="IW662_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ0867250.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2112 a          962 c         1211 g         1731 t
ORIGIN      
        1 gaaaaccgcc cgacaataaa tttcattaac tcagttgacc gaaaatgaag tagataccaa
       61 aaatttatca agagagcaag tagagaagtg agtatattca taagagaaat agctgtaacg
      121 aattattagg ttaatgcaaa tgcttattta tactccgtat aactttctca gaaaaaatga
      181 taccaataat gacttgctag tactgtaaat ccgtataata tcatctaatc aaaatggtgt
      241 tccatttgca tctgtcatcg taatcgatcc ttcgggtgac actcgataaa aaccaacgct
      301 tccagatcct ccttgggaac ggatttgttt gctggaagct gtgaacaaat aatctcgatc
      361 aattttttga taaaagctaa taacaatatc gtcactttgt aattcggaac ggtgggcaat
      421 aagaaataga agtgctttgt cagagttttc taatggaaga gaaggtacag tgctttcttg
      481 catttcagat gttgaagaaa tgctaaacga ctgaatactt gattggctat gttgcgaatc
      541 tacattacca gtagaagtat tagtaatgga agtagtagaa gtacttttag ttgaaccaac
      601 ctcagttgtt gaaatggctg ctgaagattc tttaacagaa ttctttgttt tatatccaca
      661 ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa atccctttat gcatgatagc
      721 tcctcctaaa atagattatc tttattcagt atatcactta aacaaagaaa agcagatggt
      781 ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg aagataataa cttttttaat
      841 aattatagct ttctaatatc tttcatcaac ttttctagta atcattggga aaaatttcaa
      901 gcagtagatc gcacaaatac ctacgacgat ataaatgatc ttcgcaaaaa tagttgttga
      961 accaccagca atcatagcta ctaaatcaag ttcgaacaag cctaccaaca gccagttcaa
     1021 ccctcccaca atcaataaag ttaatgcaat agcatctaag ttttcattag aattctcctt
     1081 tcttaaatga taaaacaatt atatatcaaa aagagaaaca ttgcttatat aacgctcagg
     1141 aattttagca atctatgcaa aaagatgaaa attttttttg ctcttccatt agtacacatt
     1201 ttttagctga agctgtaaat ttcatgtctt aatttttttt agtgagcttt tatactggat
     1261 ttgtaagaaa aaagaaggag aggatcacat atgtcggatt ggttggatat tgcaggaaaa
     1321 gtagtaattg taactggagg ttcctcagga attggaagaa gtatcgtcga aaatttgttg
     1381 aagcagaatg cacaagtagc gaattttgat gtaactgaat gccgtataca gcatgaaaac
     1441 ctactatctt taaaagtcga tgtgtcttct aaaacggaca tagaagaagg gatatataaa
     1501 gtaatgaaac atttcaagac gatcgatggt ctagtcaata atgcaggaat aaatattcct
     1561 tctcttctaa tagatagaaa tcatccaaaa agcaagtatg aattatcaga acaagttttt
     1621 gataagatga tcgctgtcaa tcaaaaaagt gtatatttga tgagccaagc agttggaaga
     1681 atcttggtgc aaaagggatc aggagtgatc gtgaatcttt cttctgaatc aggattggaa
     1741 ggatcagaag gacagagctg ttatgctgct acaaaagcag cgatgaacag ttttactcgt
     1801 tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg taggtgttgc accagggata
     1861 ctggaagaaa ccggtttgag aacacaagaa tatgaagaag ctctttctta tacgagaggt
     1921 ataagcgtcg aacagctgag aaacggttac agtcgaacaa gtacgatccc tctaggaagg
     1981 agtggtaagc tgcaagaagt ggcggatcta gtctgctatt atctttcaga aagatcaagt
     2041 tatatcactg gagtcacgac taatatttca ggaggtaaaa caagaggata aaggataggg
     2101 gggacgcacg tgacgctagt aaacagatgg taccaattat tgaaattact agtcactcac
     2161 aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa ccagtaatca aaccataaaa
     2221 aaagatattg gggagttgaa tgaagaaatt gccggtattg caaaaatcat ccaaaaaaac
     2281 aaacattttg aacttgaaat ttatgatttt gatttttttg atgagatcat gcaaggaaaa
     2341 ttgaaaacag ccagtgattt caattcggca tccaaaagaa tcgcttattt actaaatagg
     2401 ctgatcgaaa cagaggattt cttacgtatg gatgatttgt ctgaagaagt cggtgtcagc
     2461 agaggaacag tggtcaagga tctaaagaca ttaaaagaga tgatcaatga ttttgatgta
     2521 aagattacag gcacaccaaa taaaggacta agaataatag gagatgagct agaattacga
     2581 ctacttttat tttattttgt ttctccctac ttttcagaga cgaaaacaga acagtttcta
     2641 gaagaaaaac aagcaatcaa acattttcag aagaagacga atgctagtaa acaagaaatc
     2701 acccttttaa cgaaagttat cggtatttct ttgaatagaa tctctagtaa ctatctgttg
     2761 aagaagccca tcaatcgtta cagtgggtgt tttgaatata tccaagaatt caatgaactg
     2821 atcaatcagt tggaagaaat ctatgaacta actttgagcc aatatgaaaa agattttatc
     2881 agttttcctt tgaacatcat caacacaagt ataacgatga taaggaaagc taactctgat
     2941 ttgcgcttat atttcgaccc catgatgatg cgtattcgac gattagatct cattgatctg
     3001 gatgagtcct tcttatatca agagatgaaa cagcacctct ctttattgat aaatcgggta
     3061 atttttcgtt atcgattgac tgatttattt tatggtgaga tagaaaaaaa gtatcctttt
     3121 tcttaccaaa tcgctagcga atgcatacaa gcattagaag ctatcttgca acataagatc
     3181 tctaaagtag agatcagcta cctaacgttg tatttggagt tagcatttcg atatcaaaaa
     3241 gagcattttt cctctaaaaa agtagccatt gttcatacga caggaaaagg aacagcatta
     3301 atgatccaaa gacaaatcaa aaggatattg gggagggata tacaaataac acatgtttca
     3361 gaagaacaat atgaaaaagt agatctaaac aaatatttcg ctgtttttac aacgattccg
     3421 ttaaaaaatg taaatgaaga gacaccggtg atccatttga ctaatctttt tgatgactct
     3481 tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat tagccgggtt tcattctact
     3541 gtttttttct atcaaatatg gaaagaaaaa aagacgtatc aagaatattt aaacaaatta
     3601 gtcgctcctt tgatcagaag ctattatgtg gatgaagaat ttgaaaaaag gatcttgaaa
     3661 agagaatatc agcaaagcac atttgttaac aaagaatttg cttttcctca cacaaccaac
     3721 cagctatctc aacacattgt tttatcagta gggctaataa ctgatcaagg tttagcagaa
     3781 acaggaggaa atggagttat ttttttattt gccatacctg aagaaatgac ggctgaagtt
     3841 gagaatgaat tatttgagct atacgatatc ctatttgaac tcataaaaat gggaatagat
     3901 gagtttattg aattagcacc gacacaagaa tcatttttaa aactaatcaa aagtaaagga
     3961 gagagacttc atgaatgata tgtatctatt catagttttc gcagcttcag cttatgtgct
     4021 gcaagcagtt atcagttgga agcaactaaa gcatttcaat caaacatata gcgaactgcg
     4081 aagaaaagga aaagtagcga ttggtaaacg acctgggaaa atccgttcag gcacgattgt
     4141 tatgctagct atagacgatc atgatcaagt tttagaagtc agaaaaatgc aaggggttac
     4201 atctttggca aaatttcatt cattacctgt ctatgcgggt gaagatatcc attacatcga
     4261 tcgttaccat ccctcagttc gagcggaaga tcgtttgaca caagcagctt tagaaaatgc
     4321 cagagaagta tacttacgaa tagaggcagg aaatgaaaaa agtgaacaac cttcattgcc
     4381 ttttattgct atgctcaata attttcggat atggaaaagt acgatcaaag aaaaaaggag
     4441 tgacaaacga tggatatcat aacaaaattt gctgagggat ttatgaggct gttccaaaca
     4501 ggtgcagaaa cattcataag ctggatgaca ggaatcgtac cagttgtctt gatgctgatg
     4561 gttgcaatga atacgctgat tgctttttta ggtgaagacc gagtaacgaa agtagccaaa
     4621 ttatcagcga aaaatccgct tactcgttac cttgtgttac cgttcttatc tgcttttatg
     4681 ttgggaaatc ctatgtcatt tacaatggct cgtttccttc cagaatacta caaaccaagc
     4741 tattatgctg ctcaagctca attttgtcat acgagcaatg gtgtattccc ccatattaat
     4801 cctggagaat tgtttgtatg gatggggatt gctcaaggga tcgaaacctt agggctaaat
     4861 tcgatggagc tggctatccg atacttgctt gtgggactgc tgatgaactt tattggtggt
     4921 tggataactg attttactac tggatttgta tgcagacagc aaggaatcgt tcttagcaaa
     4981 aaagtagagc tttcagttga ttagagaaag gagagtttta aatgtatggt gatattaaga
     5041 tcgtaagagg aaacggcggg tatggaggtc ctttgattat taggccgaca aaagataaac
     5101 acaagttcat ctatgtcaca ggtggaggtg agaggccaat gatcgtagat aaaatagaag
     5161 aactgacagg tatggagtct atcaatgggt tcaaaacatc aatacctgat agtgaaatcg
     5221 cgcttgcaat cgttgactgt ggagggactc ttagatgtgg aatttatcct aaaaaaggga
     5281 taccaaccat caatatcgtt cccacaggaa aaagtggccc attagcacaa tatatcactg
     5341 aagatattta tgtttcagca gtagggttag aacaaataca gccactaatg gaaactgatg
     5401 ataacaaaga aacagtaaat actgcatctc cagataaaaa gtacaaatat gatacttcta
     5461 aaaagataac cgagcaaaaa gcagagcagc aaaactggat agcaaaaatt ggaatgggcg
     5521 cgggaaaagt agttgctaca ttcaatcaat cagcaagaga agcagtagat acgatgatcc
     5581 ggacagtgat cccttttatg gctttcgttt cccttttgat tggtgtgata caaggttctg
     5641 ggatcggtaa tgtatttgct aacattatgg cacctttagc aggaaatgca gttggattaa
     5701 caatcattgg ctttatttgt tcactgccat ttttatctcc tttgcttggt ccaggggcag
     5761 ttattagcca agtgattgga acattgatcg gtgtagagat tgggaaagga aatattcctc
     5821 cccaattagc attaccggca ctatttgcta tcaatactca aaatgcatgt gattttattc
     5881 cagtcggtct aggtttggaa gaagcaaaag ctgagacagt agaagtaggt gtaccatcag
     5941 ttctttattc aagatttctt aacggcatcc cacgagttat cattgcttgg gtagcaagtt
     6001 ttggattata tagctg
//
LOCUS       DACEKH010000120         6004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEKH010000120 DACEKH010000000
VERSION     DACEKH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.124"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            771..1505
                     /locus_tag="IW662_002453"
     CDS             771..1505
                     /locus_tag="IW662_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0867251.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1521..2690
                     /locus_tag="IW662_002454"
     CDS             1521..2690
                     /locus_tag="IW662_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0867252.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2706..3701
                     /gene="lacD"
                     /locus_tag="IW662_002455"
     CDS             2706..3701
                     /gene="lacD"
                     /locus_tag="IW662_002455"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0867253.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3780..4706
                     /locus_tag="IW662_002456"
     CDS             3780..4706
                     /locus_tag="IW662_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ0867254.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4725..5975
                     /locus_tag="IW662_002457"
     CDS             4725..5975
                     /locus_tag="IW662_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0867255.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2009 a          984 c         1228 g         1783 t
ORIGIN      
        1 ctttaactgt tagctcctgc cgaagatcgt cacatccgag gaggctcatt gtcaaggaca
       61 atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa aaaactgttg
      121 ccattccaaa gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg
      181 actaaacgaa gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt
      241 cagataaact ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct
      301 taagagagaa cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt
      361 tattctcata gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat
      421 ttgaaaccaa agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac
      481 tggtaagtag tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa
      541 aaaaagatac cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa
      601 agttggaaaa ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata
      661 ttaacaagac taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa
      721 taccactact atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata
      781 cctttcaatc aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa
      841 acagtatggt cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg
      901 ttagtcgcac gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc
      961 gacaacatgg aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg
     1021 gcgcatatag ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat
     1081 tggccttcaa gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag
     1141 gtgagccttt atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg
     1201 agaaatccta tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga
     1261 aacctttata taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg
     1321 aattttatgc cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat
     1381 caccagtgct ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta
     1441 cattaagtgt agcgcgggct gataaatttc attataagat caggcatctg cgttccacta
     1501 actgaattgg aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag
     1561 cagaaaatac tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc
     1621 gttatcaaga aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg
     1681 gagagcgacc aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata
     1741 cactaattcc gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg
     1801 gttcgaccga tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct
     1861 tggtctcatt tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca
     1921 atcagctggt caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg
     1981 ctacacaaat gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg
     2041 acgctggttt tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt
     2101 ttgatcaagc aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag
     2161 aagaagtcat ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca
     2221 tcatttactt aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc
     2281 tcgagttaac tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg
     2341 gtcccaaatc ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat
     2401 atacgcagaa atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac
     2461 gcgttgttgg aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg
     2521 cagattcgtt actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga
     2581 tcgcattact ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa
     2641 cagtcaatcg tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt
     2701 gaagcatgtt agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag
     2761 aaggcattat taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg
     2821 ctttagggat ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag
     2881 aattgactcc ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac
     2941 agaagcgtgc agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa
     3001 ccgtacccgg ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt
     3061 taggtgcgga tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca
     3121 atcggcaaaa gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc
     3181 cattttattt agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct
     3241 atgccaaagc aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt
     3301 ataaagtaga tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg
     3361 caacagggga gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg
     3421 atgcaacaga tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg
     3481 agacattgcg ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag
     3541 caacttggaa agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat
     3601 ggctgacaga acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg
     3661 ccatcccatg gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta
     3721 gtgataagag atatgtgtga ttataattta tcattattga aactgggagg atattttata
     3781 tgaataaaag attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat
     3841 taaagcaggc aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta
     3901 gctgtcctta catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg
     3961 atttaatttt gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa
     4021 ctctaaaaga gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa
     4081 atttagaacc tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg
     4141 gtagaatcgt ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt
     4201 gtttaactgg aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat
     4261 tatccaaatc aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg
     4321 atgaaccaat tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt
     4381 tattagttcc tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg
     4441 ttgattatat aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa
     4501 cggctaatgg aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag
     4561 catcaaaagc atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc
     4621 ttccagaaaa tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta
     4681 tgttaggaaa atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc
     4741 aaaaaatagc tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg
     4801 ctgcagttat gccattaatc attattggtt ccttttttat gttattagca aatttcccga
     4861 tagaaggatt tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat
     4921 cagattcaac atttggtatt attggattag ctgttaccta ctctttggga cacaatctag
     4981 caaagactta tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt
     5041 tacttacacc tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta
     5101 cctcgggtct ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt
     5161 ttgtacttaa aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag
     5221 ctttcgttgc tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa
     5281 tttgttataa acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac
     5341 cactatcttt actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt
     5401 tcttttggat gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat
     5461 tattgcaaaa ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca
     5521 ttataactta tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg
     5581 cacttgccat tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt
     5641 tatcaatcgc tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta
     5701 tattaaatat aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta
     5761 cttatttttc aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga
     5821 caactccacc gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga
     5881 ttcaagtaat aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg
     5941 ataatgccaa gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta
     6001 gtta
//
LOCUS       DACEKH010000121         5954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEKH010000121 DACEKH010000000
VERSION     DACEKH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.125"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW662_002458"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW662_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0867256.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="IW662_002459"
     CDS             808..1242
                     /locus_tag="IW662_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0867257.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="IW662_002460"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="IW662_002460"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0867258.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="IW662_002461"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="IW662_002461"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ0867259.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
BASE COUNT         2116 a         1025 c         1333 g         1480 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt atta
//
LOCUS       DACEKH010000122         5925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEKH010000122 DACEKH010000000
VERSION     DACEKH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.126"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(76..1170)
                     /locus_tag="IW662_002462"
     CDS             complement(76..1170)
                     /locus_tag="IW662_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0867260.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="IW662_002463"
     CDS             1359..2120
                     /locus_tag="IW662_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0867261.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IW662_002464"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IW662_002464"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0867262.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="IW662_002465"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="IW662_002465"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ0867263.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="IW662_002466"
     CDS             complement(3760..4773)
                     /locus_tag="IW662_002466"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ0867264.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="IW662_002467"
     CDS             4938..5858
                     /locus_tag="IW662_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ0867265.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1181 c         1063 g         1875 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtc
//
LOCUS       DACEKH010000123         5906 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEKH010000123 DACEKH010000000
VERSION     DACEKH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.127"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(94..666)
                     /locus_tag="IW662_002468"
     CDS             complement(94..666)
                     /locus_tag="IW662_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0867266.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(673..1335)
                     /gene="rpe"
                     /locus_tag="IW662_002469"
     CDS             complement(673..1335)
                     /gene="rpe"
                     /locus_tag="IW662_002469"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0867267.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1351..1866)
                     /locus_tag="IW662_002470"
     CDS             complement(1351..1866)
                     /locus_tag="IW662_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ0867268.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1868..2152)
                     /locus_tag="IW662_002471"
     CDS             complement(1868..2152)
                     /locus_tag="IW662_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0867269.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2186..3514)
                     /locus_tag="IW662_002472"
     CDS             complement(2186..3514)
                     /locus_tag="IW662_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0867270.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3528..3983)
                     /locus_tag="IW662_002473"
     CDS             complement(3528..3983)
                     /locus_tag="IW662_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0867271.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3986..>5906)
                     /locus_tag="IW662_002474"
     CDS             complement(3986..>5906)
                     /locus_tag="IW662_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0867272.1"
                     /translation="KLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLEDYVVNYQVTI
                     ERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHL
                     SQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYAN
                     DMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLL
                     KGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVT
                     NNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQN
                     PLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNII
                     IICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIP
                     LVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTG
                     VQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIE
                     SNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQV
                     HDSEKLLRIFQQKGE"
BASE COUNT         1918 a         1147 c          836 g         2005 t
ORIGIN      
        1 attattaagt tatgtctaat caaataaaaa aaccattctc atatttgctg aataattagc
       61 atttagagaa tggtttttta tctttaaaat ctactactca aaatctgcat gtcgagcaaa
      121 catcgtttct gagctttcac ctgttttatc cattaaagat aacactaaac tatcaaaggt
      181 gagaaatgct aattgttcaa aacttgaacc cataggttgt gaaaaagtat cttcttctcg
      241 ttgattttca tctttggtaa tacctggtaa tacaagaaca gaatctgcta attgaccaat
      301 cgacgagtct tttttcatgg taactaaagc aatgcttaca ccactttgat ttgctttggc
      361 cgctaaattt tttaaactac ctgtttcacc tgagccagaa cagataatta ataaatctcc
      421 aggttgcgaa tgtggtgaac taatttcacc gacaaggctg actgaaaaac ctaaatgcat
      481 taagcgattc gtaaatgctt gcatggcaat tccagagcgt cctgcaccag ctagaaagat
      541 gtgtttagct tgaacaattt tctcactaaa ttgttctaac tctacagaat taatacgttt
      601 tgagttattt tgtaattcta agataatcgc ttctaaatag tttttatttt ggtctttatt
      661 ggtcatgatc ttttacttca cttcctctaa tgattgattt agtttatcaa tttgttcttg
      721 cggggtattt ttcccattga agatgaaacc gcctgataca aaaatatccg cccctgcttt
      781 agaacaaact ttaatcgttt gattatcaat cttgccatct acttcaatat catacgtata
      841 atcattttgt ttgcgtatta catctaactg ttcgatttta actactgtct cttgaataaa
      901 atgcttattc tctgttcctg gattgattgt cattactaat acttgatcga ctaaagataa
      961 tactggttca ataaaactga ctggtgttgc aggattaaaa gtaatcccta cttttgctcc
     1021 tgcttccttg actaactgaa tgaccttcat tgcgtgtttg gtagcttcaa tgtggaaggt
     1081 aacaatatct gctcctgctt ctacaaatct tgtgacaatt ttttctggtg tatcaatcat
     1141 caaatgaaca tcaaacacaa cgtctgttag tggacgaata tctttaataa cacttggtcc
     1201 aaaagccata tctggtacaa aacagccatc cattacatca atatgcaaat aatcaacatt
     1261 cttttctttt aagatttcaa ataactcttt aacttctgcg ataggtgcat ttaacaatga
     1321 tggagcaatt gtcattctct attcccccgt ttattctccc ataatcatga gattgattgt
     1381 tctagttcgc tctcttagtt gatcccaatc gacaaaatag acacggccca tttctcctaa
     1441 taaaagttta ccttcttgaa caattaaagt cattgagttg gttccataaa tagatgaacg
     1501 taaatgtgca tcggtattta gcatgaccca tttttctgca gggtaattgg gatcagataa
     1561 tcttgttcca aaatcaatgt gttttggccc cggactgttg tattgatttt cactcgtcat
     1621 ttttggaata atcgtttcca ttacattgtt gatgtctaca tgtaaataat catcaccaaa
     1681 aaagtttgtg tcatgcatcg tttcttcaaa aaagagtgag caagttgtat gcgggctcga
     1741 aattactaac attccattct caatacctgc tgtatcaact gtatctaaaa gttcttttcg
     1801 aatatcatga tacgaaggac gccctgaaac tgtttcaata gtcaaagtgt catgatgaaa
     1861 acgcatatta aacacctaat ctttctttta catctgcata aatggcatct gtaccaatac
     1921 ctgttaataa gcccaagcca ttcgttactt tagctttaat tacatcaggg acaattgttg
     1981 tacttactac agcatcatat tcatcaattt tatttagctc atcagcaatt ttagattgat
     2041 aaattttcac ttgatctgtt aatccttctt cttccaacca ttttgataat tttgctactg
     2101 caattgtaga agtagcatga cctgctccac aaataactaa taattttttc atattgatct
     2161 cttctttctt attttaattt tatgattaag ctttcttcac tttgttcatg tagatagaag
     2221 cacctactaa aacaactcct aatccaccta ttccaattac accaaagtaa gatgctaata
     2281 aaacaaacaa ccctactggc cataatccac ataacaacat agttactaat cctgctgtcc
     2341 ccacatcgaa acttgctaat gtaaacacat cagtgataat tggtgccaac catgttgcca
     2401 tatataatgt tgtaacaaca tacaacgaac atcctactaa tgatctcaca atatttcctt
     2461 taaaataagg aatcattaaa cagatggcga aagcaaagaa tgctaaatct ccaaaaggta
     2521 atactttatt tcctggaaga attagtgcta gcaataatga gattggtaca agcaatagag
     2581 aagttgacat aatagttgga tgacccacag ttaacgctgc atccattcca atgtttactt
     2641 ggcgaccatt taacttttta ttcgtaaatt ctttcgccgc ttctgcaatt ggcattaaac
     2701 cttccataaa cattgctacc attttaggca taatcttcat cactgccgca gtcataattc
     2761 ctaagtttcc aattcctgct acgtcatagc ctgccataat acccactaca ataccgataa
     2821 ttaggccaat aacaactgtg tcacctaaaa caccgaaacg ttttgttaat gtttctgcat
     2881 ctgcatctat tttattaaat ccaggaatac gatcaaaaac ccaatttact ggtttggcaa
     2941 atagtactcc tgataatccc atcatatgtg tgatagagat tcctggatag ccgaaaaatt
     3001 cgctaacagt tttttgtgta cgatctgcta agaaccattg taaaattact gcgacaatcg
     3061 cgccaattaa gccaaaccaa aagtttcccg taacagattg agtaatcata cctaggaaaa
     3121 ttgcgaacca aaagttccaa atatcaacat tcaatgtttt tgtccaaccc aaaaccacaa
     3181 atatgaagtt aagaattaat gaaattggaa tcattaaaat tcccaatgat gtagagtaag
     3241 ccaaaggtcc accaacacct acgccaatat ctaggatatt caggccacca ccgtattgtt
     3301 cccccatttg ttgaattgct gttcctaaac catctgataa taaacttaaa actaatccta
     3361 aaccaataga accgatacca acagtaacgc cggaaacaaa tgctttacca ggttttaatc
     3421 caaataatat cccgattaca aaaataatca gtggtaaaaa gacgtttgac cctaatccaa
     3481 taaaccattc aaaggcattt aatacaatat ccatctctct attcctccta ataaactcct
     3541 gcagaatgta ctaactcaat aaatgcttct ttcgtggtac atgcaagtaa ttcatttact
     3601 ttttcttcat tttgaaatag gctaactaag ttttgtagtg tatcaatttg ttcatgaggt
     3661 tgtgacattg ccaacataaa gactaaacgt acaggtattt catgagattt atctgtcata
     3721 tctgaaaata ccagtggttc ctttaatgag gcaaaacaga tttgagagct atttacatgt
     3781 gcggaatctg tatgtgggat agcaaaccca atttgattta cttctaatcc agttggaaat
     3841 tcttgttcac ggttggtgat accttctaag aaactatctt tcacgcaacc agcgtttttt
     3901 aattgttcag acattacttg aaacaattct ttttgatctt tcacttctaa ttgaaataaa
     3961 cataattctt tatctagata catttttatt ctcctttttg ttgaaaaatt cttagtaatt
     4021 tttcgctatc atgaacttgt aacaagcgat cgaccatttc tttttgccca ataaattggt
     4081 aaagttcttc atatactcct ctaatctcag atacatcttc ttcagctaaa ctaaccataa
     4141 tgactaattt gacttttcga tcgccccaat taatcgcatt ttttaatgct acaataaaaa
     4201 tgttggattc aatgaccatt gatggattcg catgcggtaa agcaacacca ctttctaaat
     4261 tagtattccc cattttctct ctagagaaga tagaagcacg aaactcttca gtaactaatg
     4321 aattggcttc tagttcggaa atcatcaaat ccaaacattc ttctttgctt tccacctgta
     4381 ctcctgtttt tactaaagat gattgaacaa atttagatat ggtgggaact ttaaccgctt
     4441 taggaatcag ttcattttga ataaatcgcg aaggttcttc tattaaatat ttagtataag
     4501 cttccataat attcatataa tctgttttac ttaccaatgg agataccctt actaagggta
     4561 taccacctgc atcaaaggaa agagaggaaa taatcaaatc aacttcccca atttcagcta
     4621 aatgaaactt atctaaagtt gatatttcta cattatcttt tgctggtaaa aaccgtcgaa
     4681 ctttattgta aattaattgc gcagaagaaa taccgtattg gcaaataatg atgatattat
     4741 ttgctttgga ctgatcttca atagcaatct gaaaatgaat caaaattaag gaaacttcat
     4801 cgtccgttaa agtcacatca aattcttctt caaggatgga catggcgtac catactatgc
     4861 taaagagttc tgaatattga gatttgatat tttctaataa cggattttga attttaatac
     4921 ctttttttaa acgaaggacc attgctggca catgatacaa taaggattga tataatcgat
     4981 cattttgagt caaatcaatt ttttcaatct cactcattct ttcaatcaat ttatgaacgc
     5041 tcatagcgta ctcttcattg ctggttttta aattatttgt cacacgatgt gcaaatagtt
     5101 gacgacatag atattcttta tctgcgtcat aaaatggaag attcaatttt gccttcaatt
     5161 cttcaattaa accatttgcc acaatgtacg tttccatata gcgaatgcta ttgaagagga
     5221 attcttcttc aacagttaaa tgatgacctt tcagtaaacg gttacattgg ataactaaag
     5281 tagaaatcaa tgaccgaaca tagtaatcag aaaccttttc agtcagttca ctgtaatcat
     5341 tgaatagcaa atcatgaatg acactaataa cttttgaatc aaacaacata tttaaaactt
     5401 gaaaaaatgt ttttgattcc atgtcattcg cataatagaa tacaagttgc ttgattgctc
     5461 gttgaattgc ttgttcatta ccagtaaaac taattccttc tgcagatgac tgcaacttaa
     5521 cttctccact gccaataatt ttattgataa ttctcaggtc attgtataac gatgttttac
     5581 taaccataaa actttgagac aaatgagtta aagaaacagg cttatcacca aacaaaactt
     5641 ctttaatgat ctcgacacgt cttttttctg gagaataaat atctttttca agataatttt
     5701 ctaccgatga aatcaaatgt tcaacgtttt tcctatcacc tatgagtttg atacccaaag
     5761 aaggtaatcg ctcaattgtt acttggtaat taactacata atcttccaac aattcaatat
     5821 cttgataaat ggtcttggtc gacactgcca atttatcact aaaaaaagaa gcgggtttaa
     5881 agtcttcttg tcgacttaat aatttt
//
LOCUS       DACEKH010000124         5774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEKH010000124 DACEKH010000000
VERSION     DACEKH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.128"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..193)
                     /locus_tag="IW662_002475"
     CDS             complement(<1..193)
                     /locus_tag="IW662_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867273.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFE"
     gene            complement(736..2886)
                     /locus_tag="IW662_002476"
                     /pseudo
     CDS             complement(736..2886)
                     /locus_tag="IW662_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3032..3607
                     /locus_tag="IW662_002477"
     CDS             3032..3607
                     /locus_tag="IW662_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0867274.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3821..4516
                     /gene="vanR"
                     /locus_tag="IW662_002478"
     CDS             3821..4516
                     /gene="vanR"
                     /locus_tag="IW662_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0867275.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4494..5648
                     /locus_tag="IW662_002479"
     CDS             4494..5648
                     /locus_tag="IW662_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0867276.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1802 a         1107 c         1097 g         1768 t
ORIGIN      
        1 gttcaaaatc taaaatcaac acatcatggg gagtgtgata ataaatattt tcccttaatg
       61 taccaacaat ggtaattcgg tcaattgacc attgcatttt taagggggtg cacatagttt
      121 gtgcatttaa ccgcctgtta gaaatacggt taagtttttc ccactcattt acgattatgc
      181 cttttttact caaaaatagc tcctcctcaa cgaaaaaaga gattcttgga aagaatttcc
      241 agagttcacg taactctaaa aatccacatc caaaaatctc tagcttgaag aaacctaaaa
      301 atattcgtat aatgaacttg agttacggaa ctaggtgaag tagtttccgt aaaggtcgag
      361 agtgcgtcaa cactgctcga ctattttttt actctttttt ctcattttct ttgattatag
      421 cactcctcta gaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc
      481 tcctcctgcc gaaatagaat ggcgctgcca gcatttattt cgttcagcta tcggctacac
      541 aatctaacac gaccgttccg ctaccagtcg ccttacaggc tccattttcg ctcctctaac
      601 gtgtttcacc gattgtgccg ataactaaac gaaataaacg ctaaaaccgc tcagaacgca
      661 aaatatgcca ttttagagct attgtagaaa atatagtttg aaatgcaatt ttcttctttc
      721 agctttttat ttcccacttg taaatcggta gatggtggct ccttttccag ttccataatg
      781 tggatttgca tctgcatgta gtgatgaaac acctagctgc attctcatac catctgacga
      841 agatgttgta ccgtcgcccc aatagaaagg caattgtaat ttatgatgaa agtttactaa
      901 tatggcttgg gctttattca tggcatcttc atacatgcgc cattgagata cattggctag
      961 ttgcttatat gtaagtccgg gtgtggcttc ggccatcttg ctcaagccaa tattcattcc
     1021 cattcctaaa agggcagcca tgataatgat tgtttcttcc ttatctggtt ttcgattatt
     1081 ggaagcatga gtgaattgct catgaaatcc tgttatatgg gccacatcca tgagtaaatc
     1141 agttaatttt attcttggta gcatctgata aaggcttgca ctaaattttt ttgcttcttc
     1201 tggaacatct ttttctaagc gtgcaagtga tagctttcct ttttcaagag aaaccccatc
     1261 taacttattg gaattggcag ctaaccactt taacctttca ttaaagctgc tggttctctc
     1321 cgttatataa tcttcgaatg ataaactaac tgataatctc gtattcccct tcgattgatt
     1381 ccatgtatct tccgaaaaca aatattcctc aaaatcccta tattgtctgc tgccaacaat
     1441 ggaaacatct cctgcccgaa catgctcccg aagttctgtt aaaacagcca tttcatagta
     1501 atgacgatta attgttgtac catcatcctc gtataaatgt cttttccatc gttttgaaat
     1561 aaaatccaca ggtgagtcat caggcacttt tcgctttcca gattcgttca ttcctcggat
     1621 aatctcaaca gcttgtaaaa gtggctcatt tgcctttgta gaatgaaatt ccaatactct
     1681 taatagcgtt ggcgtatatt ttcttagtga ataaaaccgt ttttgcagta agtctaaata
     1741 atcatagtcg gcaggacgtg caagttcctg agcctcttct actgaagaga caaaggtatt
     1801 ccattcaata accgattcta aaaccttaaa aacgtctaat ttttcctctc ttgctttaat
     1861 taatgcttgt ccgatgttcg taaagtgtat aactttctca tttagctttt taccgttttg
     1921 tttctggatt tcctcttgag ccttacgacc ttttgataac aaactaagta tttgcctatc
     1981 atgaatttca aacgctttat ccgttagctc ctgagtaagt tgtaataaat agatggttaa
     2041 tatcgaataa cgtttatttt cttgaaagtc acggaatgca tacggctcgt atcttgagcc
     2101 taagcgagac agctgcaaca ggcggttacg gtgcaaatga ctaatttgca ctgtttctaa
     2161 atccattcct cgtatgtatt cgagtcgttc tattattttt agaaaagttt cgggtgaagg
     2221 atgacccggt ggctctttta accaacccaa tatcgtttta ttggattcgg atggatgctg
     2281 cgaggtaata atcccttcaa gcttttcttt ttgctcattt gttagagatt tactaaccgt
     2341 attaaatagc ttcttttcag ccattgccct tgcttcccac accattcttt caagtgtagt
     2401 gatagcaggc agtataattt tgttttttct tagaaaatct atgcattcat gcagtagatg
     2461 aatggcatca ccattttcca aagctaattg atgaaggtac ttaaatgtca ttcgatattc
     2521 actcagggta aaagttacaa agtcgtattc acttcgaatt tctttcaaat gatcccaaag
     2581 tgtattttcc ctttgaggat aatgatcaag cgaggatgga ctaacaccaa tctgtttcga
     2641 tatatattgt atgaccgaat ctgggatgct tttgatatga gtgtatggcc aaccgggata
     2701 ccgaagaaca gctaattgaa cagcaaatcc taaacggttt tcttccctcc ttcgcttatt
     2761 aactatttct aaatcccgtt tggaaaaagt gaagtaggtc cccagtatcc attcatcttc
     2821 agggatttgc ataaaagcct gtctctgttc cggtgtaagc aattctctac ctctcgcaat
     2881 tttcattcag tatcattcca tttctgtatt ttcaatttat tagttcaatt atatatcaat
     2941 agagtgtact ctattgatac aaatgtagta gactgataaa atcatagtta agagcgtctc
     3001 ataagacttg tctcaaaaat gaggtgatat tttgcggaaa atcggttata ttcgtgtcag
     3061 ttcgactaac cagaatcctt caagacaatt tcagcagttg aacgagatcg gaatggatat
     3121 tatatatgaa gagaaagttt caggagcaac aaaggatcgc gagcaacttc aaaaagtgtt
     3181 agacgattta caggaagatg acatcattta tgttacagac ttaactcgaa tcactcgtag
     3241 tacacaagat ctatttgaat taatcgataa catacgagat aaaaaggcaa gtttaaaatc
     3301 actaaaagat acatggcttg atttatcaga agataatcca tacagccaat tcttaattac
     3361 tgtaatggct ggtgttaacc aattagagcg agatcttatt cggatgagac aacgtgaagg
     3421 gattgaattg gctaagaaag aaggaaagtt taaaggtcga ttaaagaagt atcataaaaa
     3481 tcacgcagga atgaattatg cggtaaagct atataaagaa ggaaatatga ctgtaaatca
     3541 aatttgtgaa attactaatg tatctagggc ttcattatac aggaaattat cagaagtgaa
     3601 taattagcca ttctgtattc cgctaatggg caatattttt aaagaagaaa aggaaactat
     3661 aaaatattaa cagcctccta gcgatgccga aaagcccttt gataaaaaaa gaatcatcat
     3721 cttaagaaat tcttagtcat ttattatgta aatgcttata aattcggccc tataatctga
     3781 taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata aaatacttat
     3841 tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa acgagaatta
     3901 tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca agtctgagat
     3961 tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta tctgtcaaaa
     4021 aataagggac aagcacacct atccgattat catgctgacc gggaaagata cagaggtaga
     4081 taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct ttcgcccact
     4141 ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca gtggagtaaa
     4201 ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta acacccatga
     4261 gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa tactgcgaat
     4321 cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg agatatgggg
     4381 cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc atttgcgcga
     4441 aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg gggttggtta
     4501 taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt tacatgtata
     4561 tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca atgatccgag
     4621 ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta aatcacctgg
     4681 acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt atttatgtgg
     4741 cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc gcaaaatact
     4801 ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa caaattgagc
     4861 tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg actctggaaa
     4921 agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt atgtacttgg
     4981 cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg cttgacgagg
     5041 ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg gacaaagcgt
     5101 atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac ctacaaacga
     5161 taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg accgatgaat
     5221 tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc gaggatctga
     5281 ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg aaaaacgccg
     5341 ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc ggggatgtgg
     5401 tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct gccatatttg
     5461 aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg ggacttggat
     5521 tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg gaaagcaatg
     5581 ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt gataaaagga
     5641 ggtcctaaga gatgtatata attttggctc tttgtcaaat gatgttggta aagagaaatc
     5701 ttagaataat tggagatcaa gtggatcagt tcctcttgat cttttttagt tgaaaatcaa
     5761 tgcgttgcca tgac
//
LOCUS       DACEKH010000125         5586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEKH010000125 DACEKH010000000
VERSION     DACEKH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.129"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            142..2202
                     /locus_tag="IW662_002480"
     CDS             142..2202
                     /locus_tag="IW662_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867277.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2285..2854
                     /locus_tag="IW662_002481"
     CDS             2285..2854
                     /locus_tag="IW662_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0867278.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3029..3835)
                     /locus_tag="IW662_002482"
     CDS             complement(3029..3835)
                     /locus_tag="IW662_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867279.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3973..5487
                     /locus_tag="IW662_002483"
     CDS             3973..5487
                     /locus_tag="IW662_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ0867280.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1988 a          967 c         1129 g         1502 t
ORIGIN      
        1 ccgattttct tgaaacaata aaaaatcaaa aaaaatactc tcctaatagt ccatcttctt
       61 ttgattttat ttatataatg aaatcatctt atgaaaatta ctacttcatg tttagacaag
      121 agtagggagg actaagaata aatgtgttca aaaagacgtt caaaacaact aaataaacac
      181 gcttgtcgtt tagataagga acggtcgtgt tctgaaaaac actacgttaa gtttatgatg
      241 cgcacgatgc tgatcagttt atctgtaggt gtctatatgc atgtacccgt tcaggcaacg
      301 acacaaacca tcaaggtcgt acgtacctct gtttctgaga ccactgattc ccctatcact
      361 attactcata ctttcactga tgcaaatgga aaacagctac ttcctgcatt gacttcctat
      421 aataaagtca gttatcctcc aacgataaac cactactggt taattcctaa caaaacatta
      481 tggacatcgg aaaccggaag cagtgtgccg ctttctatct tttccgatat gactgatcct
      541 acaaaaggtg atcagttaaa acagatcact gatattttga atcatgcttt gacagatata
      601 aaggacggtg gaagcgcaca agtttcttat gtatatgaaa aagattattc acagtttgaa
      661 agaaagaaaa tcgatgttct ggcagggaca aaagtttcga tatctgatct ggtagaaagg
      721 ctgaagaacg cagatggaac acaaggagat atgtccaaag taatttcaaa caatgggaat
      781 atcgtggata cgactaatac aggaacatct actatttcct tgtcctattt tgatccaatt
      841 gctttaaaaa caatgactgc atctgctgtg atccatgttc gtgctgatca aacagctatc
      901 aaaggaaaag atcttcactt aagaacagga gagagttacg cccctaaaga tcttgttgaa
      961 tatgtaacaa aaagcgatgg aacttcagga aataaagaag aaatcaaaat gaacgggaca
     1021 gtagatacta caactccggg agaatatccc gttgtattaa cttatataga ctcgaaaaca
     1081 tcaaatgtgg cagaaacaac agctgtagtt tatgtagaag catctgctgc ttcatcgact
     1141 gtcatcactc attttataga tagtcaaaca aataaagaac ttcaagcaga aacacatatg
     1201 agtagtgtaa atcgtattcc tgatttgcgc ctacctgctg aattttctga tcgagtgcca
     1261 aatatagatt tatcgactta tgaacaaaat gggaaagtca cttctatttc tgattggatg
     1321 caagcaaacg gattagacag aaaaagtcac tggtcggaga tccttgaagt acttcactca
     1381 gaactgatga ataataggaa gcaatcagga ggggaaatta cctttactta tgtttatata
     1441 ccagacaatc gttcattaga aggtcatgat cttattgttc atccaaacga aaaaataaac
     1501 aaagaagagt tgatccaatc agcaacaaat tccttaggta aacccgtacc gttaaaggat
     1561 gtatcggtta caatcaatga agtaccattg gaggatggtt atttgaccaa acaaacaggt
     1621 gattttacga ttatgtatag ttatcatgac ccgtattcac tccaagaact ccatgctcag
     1681 tcactattac atgttttacc attaaacaaa cagatagatc aaaaagaaca aaaaagacaa
     1741 gaagaaagct tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc aacagggaaa
     1801 gtaaaacaaa caagaagcct agagttagca agaatatcta gaaaaatctc agaatctaat
     1861 aaaacgataa ggcaagaagc tcatttggcc ccacaagttt ctggattgga aaatgaaatt
     1921 ttagagccta aaataaaaca acaatcagaa aaagaatttg ttgctaaaca gtcggaagat
     1981 cagaaggaat cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac tattgattcc
     2041 accgatgagc atctgcagcc gcaagtacca cttcctagtc cgggaacacc cgcacctggt
     2101 ggaggtattc cttcttctgc tttaggagat ttcatgcgag gtatggcagg aacatgggta
     2161 tacgctaatc gaaatgaatt tgacgcagat gactggttct gatttatatc tctatctgtt
     2221 atagtcaaat aagtagctga gtcatatgtt atttggcata atggagacga ggagggatag
     2281 aaaaatgtta gataaaacgt taccttacgc agaaatctgg atgacaagac cacttcaaca
     2341 aaatgttcca atcacgcctt tagcggaagg ttttgaattt cgattttatc aaaaagaaga
     2401 tgaatctgct tgggcggaga ttgagacttc cgttggggaa tttcaaacga cagatgaagc
     2461 acttgagtat tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa gaaggatgtt
     2521 ttttgtagag acaaaaaaag gagaaaaagt agcaacctgt accgcttggc gaaaaaaatc
     2581 aagagagcaa gtgtatccgg tttttcattg gttagcagtg aaaccagatt accaaggaaa
     2641 agggctcgca aaagcattga ctgccagagt cttgcaagaa tttcctaccc tccatacgga
     2701 cggaccaatc tatcttcaca cacagacatg gagccatcag gcgattgctc tttataaaaa
     2761 aatgggtttt acttttattg ctgaaaatct agacggcaca cctaatccag attatgaaaa
     2821 agttatgcag gttttaagta aattagataa ataacgaaat ggtgaaataa gtcctcagta
     2881 ttgggataaa aaacgaaata gtcattcctc atatcttact tttttgagtt aatactatcg
     2941 gataactaat ggcatgccat acattcaaaa aagaaggggc agtgacaaaa gtattgtcac
     3001 tgccccttct cttgtttttt agcatgtttt atcgtttgat ttttttagga ataaaattct
     3061 tgatgaacgt aaatttttgg taatcgtttc gactatagag gtaaccaaat acactgaaaa
     3121 caactgctcc aataaaatta acaaataaat ccttcatagt atccatgatt cccacgtcta
     3181 agtaaccatt ttggatgatt gttttatctg ttttaccatc tgcattttga ctttcaatga
     3241 ttgtccgagt gatatctgga acatgataga cagtgtttcc gtctttactt aactccacac
     3301 tattgatttg ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat ttatctgctg
     3361 aaaattccac aaactcccac ataacaccaa ctgtcatcga aaaacagaag gaaacaactg
     3421 ctagtaataa aggagtcaag ttcatggttt ttgtattttt attgagcaaa taaacaaggg
     3481 aaaagccgac acccccagct aaaaatccgc tgaatgcatg tagaaaagta tcccacattg
     3541 aaaaatagcc ataaaaatca cttaactctc ctaaaactgt tgaactaaaa ataaaagata
     3601 acaaaatgat ttctagcata tttggaatct gtatctttaa tatcttttcc agaaaataag
     3661 ggatttcaaa taaaattaat gtcagtaata aaagcattgt atttccccag tgttggttcc
     3721 agacctgatc cactagcata ataacgacga tgatacgtaa taaaatatag attccgtttg
     3781 tgattctacc tgcagttttt gtactgttcg taaaaaaggt tttcaaatga ttcataaaat
     3841 aactccttca tgtatatcct taattatact aagaagcgtg tgtttaccaa agctatatga
     3901 cttaaagaaa aacagagttg atattgtgaa aaaaatcact taatatagta taaggagaaa
     3961 agggggatac aaatgaaaaa attagttgct ggtttattaa cagccgttgc acttcttgct
     4021 atgagcgcat gcggcgcaga tcagaaaaat acagcgcaga caagttctag tacgaaggaa
     4081 tctgccgaag tatcatcagg cgcttctgaa caagtatata cagatccaaa agaaatgaaa
     4141 gaatcttatg atgtcgtgat tattggagct ggtggtgcag ggatggcagc agctatcgaa
     4201 gcaaaagatg ctgggttgaa tccagtaatt ttggaaaaga tgcctgttgc cggaggaaac
     4261 acatctaaat catcaagcgg tatgaatgct tcagaaacaa aatttcaaaa agaagaagga
     4321 atcaaagact cgaatgatgc cttctatgaa gaaacgctaa aaggtggtgg aggaacgaat
     4381 gataaagaaa tgcttcgata ctttgtcgat cactctgctg atgcggtcga ttggctagat
     4441 caaaacggta tcaaactgga caacttaacg atcactggag ggatgagtga aaagaggaca
     4501 catcgtccgt ctgatggctc ggctattggt ggatacttag tggaggggtt attgaataac
     4561 gtccatgagc gggaaatccc tatttttgtc aacgcagatg tgacagacat taagaaaaat
     4621 ggggaaacgg tcaatgaagt gactgtacat gttcaaggag aagaaccaaa agaggtcacc
     4681 ggaaaagcaa tcgtagttac cacaggcggt tttggtgcca gcaaagaatt tattgaaaaa
     4741 tatcgtcccg atttagctga ttatattacg acaaatcaag aaggatcaac tggagatggt
     4801 atcaccatga ttgaaaaatt aggtggacaa accgttgata tggataaaat acaaattcat
     4861 ccaactgtgc agcaggatga gggggtcttg attggtgagg ttgttcgcgg agaaggagca
     4921 atcttggtcg atcaagaggg gaaaagattt gtcaatgaaa tggatacaag ggataaagta
     4981 tctgcagcaa ttacagcatt gcctgaaaaa tcagcttatt tgatttttga ccaaggcgtg
     5041 cgtgatcgtg ccacagctat tgaattttat gaccaaaaag gatatgtcac aactggaaag
     5101 acaatggaag aattagcaga aaaaatcgat ttaccaaaag aaacactagc aaaaacagtt
     5161 gatggatgga atacggatgt tgcggagaaa aaagatgctc aattcaaccg tgccacagcc
     5221 atggaacatg acttatcgtc tccgaattat tatgcgatca aaatcgcacc aggaatccat
     5281 tacacgatgg gcggtgtgaa aatcaatacg aacactgaag tattagataa agaaaaccag
     5341 ccaattaggg ggttatatgc tgctggtgaa gtcacaggtg gcttacatgg aaacaaccgg
     5401 atcggtggaa attcagttgc ggagatcatt atttttgggc gacaagcggg catacaagca
     5461 gctcgatttg cttcggaaaa tgaatgaaat agaacgtaaa taaaaagagg ttgtgaaatc
     5521 agcgttttga gctaataccg agggtcagct gattgaacac tgtttagtga agaggttgtg
     5581 aaaaag
//
LOCUS       DACEKH010000126         5481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEKH010000126 DACEKH010000000
VERSION     DACEKH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.130"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="IW662_002484"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="IW662_002484"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="IW662_002485"
     CDS             complement(580..1353)
                     /locus_tag="IW662_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0867281.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="IW662_002486"
     CDS             1497..1847
                     /locus_tag="IW662_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0867282.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="IW662_002487"
     CDS             1828..2544
                     /locus_tag="IW662_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0867283.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="IW662_002488"
     CDS             2544..3992
                     /locus_tag="IW662_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0867284.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="IW662_002489"
     CDS             complement(4062..4571)
                     /locus_tag="IW662_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867285.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="IW662_002490"
     CDS             complement(4561..5286)
                     /locus_tag="IW662_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867286.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DACEKH010000127         5315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEKH010000127 DACEKH010000000
VERSION     DACEKH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.131"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            100..570
                     /locus_tag="IW662_002491"
     CDS             100..570
                     /locus_tag="IW662_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ0867287.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="IW662_002492"
     CDS             792..1895
                     /locus_tag="IW662_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0867288.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2116..3075
                     /locus_tag="IW662_002493"
     CDS             2116..3075
                     /locus_tag="IW662_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0867289.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(3631..3975)
                     /locus_tag="IW662_002494"
     CDS             complement(3631..3975)
                     /locus_tag="IW662_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867290.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(4072..4317)
                     /locus_tag="IW662_002495"
     CDS             complement(4072..4317)
                     /locus_tag="IW662_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0867291.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(4387..4641)
                     /locus_tag="IW662_002496"
     CDS             complement(4387..4641)
                     /locus_tag="IW662_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867292.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(4805..5128)
                     /locus_tag="IW662_002497"
     CDS             complement(4805..5128)
                     /locus_tag="IW662_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867293.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT         1917 a          888 c         1045 g         1465 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact tcgccgattg taaaattaag
     2041 ctagacaact aaaaaaagtc atttgtgcta cactcaaaat agttccgacg aaagaattat
     2101 aaggagtgag tacaaatgac ctataaccat cttacaccaa ctgaactcgt aatgatagaa
     2161 gcttatttca atcaatcaca acctgtttca aaagtagcca atctgttaca acgttctcga
     2221 caaactattt ataaggtcta tcgttttctt aaatctggtg gaagcgcaat gagctactat
     2281 aaacaatata agaaaaacaa acgtaactgc ggaagacatc cgattgtttt acctgatgag
     2341 caacaggaat acattcaaaa aaaggttgtt caagggtggg ctcctgatgt ccttattggt
     2401 cgagcagagt ttcctattgc ttgttcggta cgcacactgt atcgaatgtt taaagataaa
     2461 acctttgaca ctcacgattt accaatgaag ggaaaaagaa aaccaaacgg tcataaagaa
     2521 aaaagaggaa aacaagggtt tcgccggtca atccgagaca gaaagacaga ctatacccag
     2581 tttgatcatg aatttggtca cctggaaggt gacactatcg tgggtggaaa acataaaagc
     2641 gcggtcatca cattagttga acggctatct aaagtcatca ttaccttgaa acccgaaggc
     2701 agacaggcta aagacattga aaataggctg aatcaatggt ttcgttgtgt cccaaaaaac
     2761 ttatttaaat ccatcacatt tgattgtgga aaagaattct caaattggaa aaagattagt
     2821 aataccaacg atatttcaat ttattttgcg gatccaggaa caccttctca acgaggattg
     2881 aatgaacatt caaatggctt attacgtaaa gatggtttgt ataaagctat ggattttaat
     2941 ggtgtatctg aaacctttat ccaatcggtg gcatcgaaac gaaatcatat tcctagaaaa
     3001 tcattacact atcggacacc aatggaagta tttttgagtt acgtcacaaa agatgatttg
     3061 tctaacttaa tttgacaaac gaaaacaaaa aaagaaagcg ttattatttt agtctatttt
     3121 caggggggga accttatgat aaaaaaacta tttttactat caatattaac gctagcaatg
     3181 gcaggttgtt ctgactctgg cttagcaact gaactgatca caaaatcaac tgaagccacg
     3241 acagaggagt taaaatgtgg aaacgatcac agtgactaca gaggcttaaa tcgacgatgc
     3301 atgggaatat taggcagaag caattgtaag catcgatgta gtaatcgaag aaggtgtaga
     3361 agaagcaaca gaaaaatcaa aagaaagatc aaaagaagca atctgtgctg tgcatcgtat
     3421 acagacgtcg aagatgcggt acttttaaac cacgtcagga gatgttcatt tatgtctagg
     3481 gaagttggcg atgaaccaaa actagagtac gcttttagtt ttgcacgtga ggatgaagat
     3541 aaccctaaaa cttacgacat tttagcttga tctaagatgg ttaaagcttt ttaatgaagt
     3601 aagaggagat agaaggcttt tctagttata ttaggtaaga taaattcctt tttgctccaa
     3661 atttgctaaa cattctgcta gataaacttc atcgtgttcc ggataaatcg gcacgttgcg
     3721 ctctacagta ggtaaatcaa aaaatgcttc gcatgatttg ccacgataca taggattttt
     3781 ccactctggt gctgggctgt aaccacgttt ttccatttca tccatgacaa gctgatgata
     3841 ttggaataat ctataaggag aatagtgaaa aacataatcc acagtagcat gtttctttcc
     3901 ccagccattt cctcttaatg cacagcattc ccgatgttgt cctaaaagct gttgacgagg
     3961 aagctttgaa atcaattttt catgccataa tcacatagaa atgtctcctt tatatataga
     4021 ttgttaacct tagacgttaa atgaaaaaac cagcaccaag gctggaaaaa atcacttcag
     4081 tttgcttgct gtttctgagc gaccaattag gtaatcaacg gaaatatcgt aaaagtcagc
     4141 gatatagatt aattcaatga tgcttggaat ttcttctcct tcttcgtact tacgatattt
     4201 gaataaagga atacttaacg cgctagaaac agctacttga gataaagatc ggccagttct
     4261 taagaaattc agacgacaac gtaaagtatc cagtaaaata gcttcttcga tactcattta
     4321 atcacctttc attatagtag aatagttata atgactatag aaagctacac atgcaatcaa
     4381 tagtactcaa tgttttttat ttaaacgtat caaataaaga atgacgatca ataaagacaa
     4441 aaccagtcct gataattctt ctataaaaac actatcgggt tcaaagtagt tgtaattaaa
     4501 aactttccac ataaagtaga tagccggaag agtcaaaata gctaaataaa accgttcttt
     4561 gttccaattc ataagataaa caatacttgc gaccagtgcg accgcaggca cccaaaacaa
     4621 aactctcacc aacgtaatca tgtaacggac tcctcaacga ttcatataat aaaagataac
     4681 atgttattgc atacatcaca aatgaaatgc taggaaaata aaagaagaat agatcaatga
     4741 aaaatgaaaa agcagaacag ttttctgctg ataagcagga actgttccgc tgaatgtgtc
     4801 ttatctatcg agaaaaaatc aatggattga tatctaagta attcaataca tataaaaaga
     4861 ggacgatcgc attcactaaa aaggaaagaa tgatgatccc atagcgaatg gaaacatatt
     4921 taacaaatac ttttgtttga atacgggcat atctttttcc tttatagctg agcatcatga
     4981 caagaagcaa aagggtaatc acaccttgaa aaatcattcc aataaacaga attcctgagg
     5041 tttgataatt taattggaga aaataagctg ctgccgcaac taaatcgaat aaaatcgcaa
     5101 taaatggcaa aataatcact cctaacattg agaataaata aaagaataac attttttcta
     5161 aaataagcaa ataaaaatta aagtaacaaa atagttatga cacaagtaaa agaaataaga
     5221 ataacaatat tattttactt atattttgaa acttgtgaga gtttgtcatt cgtgctatag
     5281 ttgaaaagta acaaaaataa attttattta tgatg
//
LOCUS       DACEKH010000128         4907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEKH010000128 DACEKH010000000
VERSION     DACEKH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.134"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(93..1055)
                     /locus_tag="IW662_002498"
     CDS             complement(93..1055)
                     /locus_tag="IW662_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0867294.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1057..2496)
                     /locus_tag="IW662_002499"
     CDS             complement(1057..2496)
                     /locus_tag="IW662_002499"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ0867295.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2681..4627
                     /locus_tag="IW662_002500"
     CDS             2681..4627
                     /locus_tag="IW662_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ0867296.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1588 a          816 c          793 g         1710 t
ORIGIN      
        1 gttgacaact cctttttgtt tacaaatcaa ttggatatct acaaattgta ttttctaacg
       61 aattttatag acattaaaaa attaatttaa cgttaaatac ttcctccaga aagtattgag
      121 attggtaaaa tataatcctt atttgtcttt tcgcctttaa tttttttcag aagaatatca
      181 acaataagac ttgcaatttc atcaattggt tgtcttattg ttgtcaattg tgggaaaaat
      241 ttctcaataa aagaggtacc atcgtatcca ataattttta aatcttctgg gatattaagt
      301 ttaagttgat gagcaatttt catagtgaga atggctgtta aatcatcaga aacaaaaata
      361 ccatctggtt tgtgatttat aatagttgac tttatttcca tttcacgacg gatagtagac
      421 agattattag gaattttgaa aatttttcca tttggatttt gaaaagaaaa acctagtgca
      481 cgtaattctg tgggagaatc cgtgttatca tttcctgtaa tcataatggt attttgacaa
      541 ccattttttt gtaaagtctt agctgcaagt tttccccctt caaagttatc tgaagaaaca
      601 atggaaacat taggagctaa atttctatca aaagcaacaa taggtgctct cacacgctca
      661 taatcagcaa ttccaagatt atggcttgat gagattatgc catcaacttg gttggcttca
      721 agcatttcaa tgtattctct ctctttagca ggatcatttt gactattgca aataatcgtt
      781 ttatatccat gtttaaataa ctctatttcg agatactcaa tgagttcaga gtaaaagata
      841 ttactaatat taggaaaaat aagaccaata agctgaggtg actttccttg aagtccacgt
      901 gctaaattat ttggtttata accaagagtt ttcatagctt catgaacttt ttgttttgtt
      961 ttttctgata aatatccctt attattaatg acgcgtgaaa cggttgttgg gctaactcca
     1021 gctaattcag cgacatcagt taattttgct accattttaa tattttaatt caaagtattt
     1081 tccagtaggt tcgcctgatt tgatttgaat accattttgg tgattttcag ggaatacacg
     1141 actagtaaac actttttctc ctttattaat aaaaatctca atgattgagt tatctacaaa
     1201 aatgttagca gttgtttctt tagtatcaat tgagcattca cgaatcgtac caaagtcagt
     1261 ggcatattga atgcctgctt tgctgcgatc taaaataatt tttccttctt tggtatctac
     1321 aatcaagcta agtccatttc ctttactatc agagaaaaga agtaactccg atttctgatt
     1381 tggaggaaaa gttagttcaa gctcataagt attatttgtt tctgcttttg ctgtaaattg
     1441 ttcagaacta gcacgtaatg atgtaatagc ttctactgga tattggtaaa gtttaccatc
     1501 ttttattgaa agttctttta caagactcat tgctccctga taatcatact tatcagttgg
     1561 ataatcgata tcaggtagtc caatccaact gacaatcaga gcacgaccat caggagtatt
     1621 gaatccttga gtcgcatacg cttcaaatcc ataatcaaga ttttgaattt ctgaagcgcc
     1681 cacaagtttt actttttcta tgtcaagcga ttgacagatc ttaaaagtat tcggatagat
     1741 attcccataa tttagctcag atttatccaa cccttgagga caataaatga gaacaggttt
     1801 tttgtctaca aatactagat ttggacactc aatcatgtat tctgaaccag tgccgccaaa
     1861 atcaaggttg ccaacttcct cccatgtttc cacgttattg tcatccgctt tgtaaagttt
     1921 gataaagcct gatttttcta aattttgtgc tccaacaatc gcatacaatt gtccttcata
     1981 attaaacatt tgtggatcac gaaaatgatc agttacatca gcaggtttat gaataagaac
     2041 attctcacct ttcgtaatgt tgtagtcttt atccatccat gctccgattt gaagtgtatc
     2101 acgttcccaa ttttcatttc gaacgtttcc tgtatagagt aagaatagat tcccctctat
     2161 ttcatatgca gaaccggaat aagcgccgtg gctatctcgt tttgtgtctg gaagaagttc
     2221 tgctcctgtt acatgaaaat gaactaaatc atctgattca ttgtgtaccc attctttcaa
     2281 cccatgagca gctccaaatg gccaattttg ataaaaaagc gtatattttc cgttaaaaaa
     2341 agaaaaacca ttaggatcgt ttaacaatcc tgttttaggt tcgatatgat agctagcacg
     2401 ccaaggtgat cgttttacat tatccttaat tttatccatc tcatctttag tccaatcaga
     2461 ataagcacga tatcgtattt ctcgaggtaa gttcatattt tactccttca atttatcttt
     2521 ttacaactag tatggtaaac gttttccgtt gaaaaatcaa tcatttcatt taaaaaaata
     2581 aactatatga caaacgtttg acatattttt aagatatgct aaaataaatt tgcatttcag
     2641 tgaaccgttt tcacgtaaaa ataaaaaagg agtgattcga atgaattaca gtaaagtagc
     2701 ttccgacgta atacaagcgg tcggaaaaga taaccttatt gcagctgcac actgcgctac
     2761 acgtcttcgt ctcgtactaa aagatgacac gaaagtgaat caaaaagctt tagacgaaaa
     2821 tccagatgtg aaaagcacat ttaaaattaa tggacaatat caagtgatta tcggtgcagg
     2881 tgatgttaat ttcgtttatg acgaacttat caaaaaaact ggactttcgg aaatttcaac
     2941 agatgattta aaacaaattg ctagtaaaga taaaaaattc aaccccatta tggcattaat
     3001 taaattatta tcagatattt ttgttccaat tatacctgcc cttgttgcag gtggtttatt
     3061 aatggcatta caaaactttt taacagctgc tggacttttt ggaccaaaat caattgaaga
     3121 aatgtatcct gcgattgaag gtatttcctc aatgatccaa ctgatgtccg ctgcaccatt
     3181 catgttctta ccaattttag taggtatttc agctgctaaa cgttttggag caaaccaatt
     3241 tcttggtgct gcaattggta tgatcatgac aacaccagat cttggtggct caactgaata
     3301 ttggaatatt tttggttatc atgtttcaca aacaaactat gcttatcaag ttattccagt
     3361 tttagcagct gtttggatat tatctatatt agaaaaattc tttcataaaa aattgccatc
     3421 atctattgac tttacattca cgccattatt atcagtaatg attactggat ttttgacttt
     3481 cacagttatt ggccctgtaa tgttattgtt gtctaatgga attacagaca ttattgtttg
     3541 gctatataac actactggat ttattggaat gggtattttt ggaggaacat attcacttat
     3601 agtcatgact ggtttacatc aatcatttcc agctattgaa actcaattac tttcagcgtg
     3661 gacaaatggt atcggacatg gagactttat ctttgtagtt gcatctatgg caaacgttgc
     3721 tcaaggtgct gcaacatttg ccatttggtt cttaactaaa aattctaaga caaaaagtct
     3781 tgcttcctct gcgggtgtct cagcacttct tggaattaca gagcctgcct tgttcggagt
     3841 taacttaaaa taccgctttc cattcttttg tgctttaatt ggttcaggaa ttgctacagc
     3901 agtagcagga ttacttaaag tagttgctgt ttcacttggt tcagcaggtt tcttgggatt
     3961 tctttcaatt aatgcaacat ctattccatt ttacgtattg tgtgaattga ttagctttgt
     4021 agttgctttt gcaattactt acttctacgg taaaacaaaa gctgctggta tctttaaagc
     4081 agaagaaatt gctgaacaaa caccagttaa ttcttcaaaa gtagcaacaa cagatccaat
     4141 atctactgat gttgaatcaa cacaaacaaa agtttcatca gaaacaattg ttagtcccat
     4201 ttctggtaaa gtgattagtc tttcttcagt aaatgatcca gtcttttcat cagaagctat
     4261 gggaaaaggg attgccatta aaccaaatgg aaacacggtc cattctccag tagatggtac
     4321 tattcaactc acgtttgaaa ctggacatgc ttatggtatt aaatcaaata atggtgctga
     4381 agttctaatt catattggta ttgatactgt atcaatggga ggaaaaggat ttacacaaaa
     4441 agttactact aatcaaaaag ttagaaaagg cgatattctt ggagcatttg accgttcaat
     4501 gattgagaaa gaaggtcttg atgatacaac aatgattatt gtgaccaata cttcggatta
     4561 ttcagaagta aatatagtag ctgaaaatac agtcactgag ggtactgatt tacttgaaat
     4621 caaataaata tatttccctg agtaaagatg gaattttctt tccaaaatca aataatatag
     4681 attaatcaaa tatttgagaa taacatttta tttaatcttt tcatatatca attgtatgaa
     4741 aaattattag aattttaaaa aatgataaaa attcaaaaag aaaaaatagt agttatttac
     4801 ttgtcatgtt actaaataaa aaaataattt aaatatgata tatagtcatt tatttagtga
     4861 tttcattcta aaataaaggg agaaatgtct atgctatacg gtgcaat
//
LOCUS       DACEKH010000129         4893 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEKH010000129 DACEKH010000000
VERSION     DACEKH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.135"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..563)
                     /locus_tag="IW662_002501"
     CDS             complement(<1..563)
                     /locus_tag="IW662_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ0867297.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="IW662_002502"
     CDS             complement(750..1400)
                     /locus_tag="IW662_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867298.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IW662_002503"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IW662_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ0867299.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="IW662_002504"
     CDS             complement(2912..3910)
                     /locus_tag="IW662_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ0867300.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IW662_002505"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IW662_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ0867301.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
BASE COUNT         1486 a         1051 c          757 g         1599 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tat
//
LOCUS       DACEKH010000130         4784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.137, whole genome shotgun sequence.
ACCESSION   DACEKH010000130 DACEKH010000000
VERSION     DACEKH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.137"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            544..1107
                     /locus_tag="IW662_002506"
     CDS             544..1107
                     /locus_tag="IW662_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0867302.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IW662_002507"
     CDS             complement(1386..1673)
                     /locus_tag="IW662_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0867303.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IW662_002508"
     CDS             complement(1663..1992)
                     /locus_tag="IW662_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0867304.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IW662_002509"
     CDS             2297..2626
                     /locus_tag="IW662_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0867305.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IW662_002510"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IW662_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ0867306.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="IW662_002511"
     CDS             complement(3551..4462)
                     /locus_tag="IW662_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ0867307.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1564 a          824 c          722 g         1674 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tctt
//
LOCUS       DACEKH010000131         4778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEKH010000131 DACEKH010000000
VERSION     DACEKH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.138"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(58..1143)
                     /gene="xerS"
                     /locus_tag="IW662_002512"
     CDS             complement(58..1143)
                     /gene="xerS"
                     /locus_tag="IW662_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ0867308.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3219..3737)
                     /locus_tag="IW662_002513"
     CDS             complement(3219..3737)
                     /locus_tag="IW662_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ0867309.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1566 a          784 c          726 g         1702 t
ORIGIN      
        1 tgggctttta tctaatttta gagacaggct tcattattat actatttttt actacattta
       61 caaagcgttc aaagcatcaa ttgtagctgc attgtctata tgagtatata aagttgttgt
      121 atttgtactt gtatgtccca attgttgggc agttaatacc aatgaatttg tctgttgata
      181 gagtctagta gccaaagtat gacgtaattt atgtggactg acttggactc tgaatccttt
      241 tgaatatttt gctaccatcc gttcgattgc aataccggat atacgcttgg cttctccttg
      301 ataaattgct agaaataaag ctttttcgtt ttctgttact ttgtatcgtt ctttccgaat
      361 agctagataa ttagctaaat attccattgc aaatgctgct atatttactg cgtctctctt
      421 atttcctttt ctagttacta ccacattatt cttttctaaa gataggtcct ctaaattaat
      481 atttacaacc tcggagagtc ttaatccaga tcctaaaatc aatgcattga tcgccaggtc
      541 tctttctaca tctcgttgat aatagatcaa agctcgtttg ctcaaagttt ttttatattc
      601 attggcaata taatctagat aattttgtgt atcttcgtct agaaataatt ttttctcgat
      661 tgcgtttgca cgggcagcta acgtagtttt atctgtgata gttgctactt gatccatagg
      721 attattttct aagtatggtt ttcctaaagg attattggaa cttttcgaaa gatatttaaa
      781 tagaaccttt aatgctgtga tatttctatt gatagtagaa gtagatagac ctaagttatt
      841 attatctttg agcttagttt ttgttgtctc tttttttaca ttttcctttg tgcgacgttt
      901 caaatgagat ttgtacaagt ttagatcata ggcagttaac tgtcttatcg aggtgattga
      961 agtatcggga acgatagact cactcagaag ccattcataa aagagcttat aattttttag
     1021 atattctaaa gtagttcgag gtgattgatc tttatcctca cgatcttgaa caaaatcttt
     1081 tatataatcg ggcatatgaa gtatgattgt atctattttt tgatatattt ctgtattttt
     1141 catgttattc atcctttcga taagtagggg agaggagtat ctctataatt ggactatacc
     1201 aattatagag tgttttgtca attggtatac tctgaaattt attaatttat ttcaaaaaag
     1261 cagataaata ggtatagatt taataattta aaataaatgg tatacatgaa taggaaagga
     1321 aacagaagga ggcaatctta attttttaaa ttgtctaaat tatatagaaa gaacaatctt
     1381 tctatataat tagacataag agagactacg gttgatttga tattaacaag cgtatatgaa
     1441 tgtatataca aataaaaaag gggagggaga agttaaagaa aattcagaga agtggggcac
     1501 tcagtagtat taacaattta aaaggaattt cttaaagaaa atgggttgca tcctggagat
     1561 acaatttttt tagataaaga aaaaattcaa gcaacaattc aaaaaaatct taactgctga
     1621 gattgatgta gcaatatctc aatcatcaac aaagtacgat cctgcctttc aaaagcttat
     1681 tgataaatgt ttattcatta taaaaataaa agaaaaattt atgtcagctt ttttcatcaa
     1741 agtggtaatg ataattcgtc tattaattgt attttttagg aaatatggta taattgtttt
     1801 aacgaaaagg taattcatga tacaccaaaa accactagct ggcgggctag tggttttctt
     1861 ttacgttcta ttcagattaa cgggtaggct tactgtcttt tttggcaata attttgcaat
     1921 caagccattt acagatgtaa tgcgcggata cttctgctaa gattgcaaaa aacatatcga
     1981 aaaggtaatt cataatacaa cctcctcccc ttgccataat aggccggggt agcagacagc
     2041 ataaatatta taccataact gaaaatttca ttttagtaca tttcaatata ttgatgaacc
     2101 agcacaataa aacatgccat gactaacaga aaaaaactta ttaaaccaga tataacttgt
     2161 aaacatccaa ctatatagaa aacgtaaatt ttctatatag ttggattaag ataacgacta
     2221 tttattttat catattttta ctatacccta tacttttact tggtatattt atgaataatc
     2281 gcaaatttac ccctatacta ctagtttgta agttagatta ataaaaatac taaagttatt
     2341 tgttctttta ttaaatacac ctacaactta taaattgatg gtatttagat aacacgaaag
     2401 aataaaaata acagctgata gtaaagtttg ttgacgattg ctactcaatc gaaagtgagt
     2461 tagtgcgtat gaacttgctt ctgaaaactt atgaaatgaa agacctttta catcttgcag
     2521 aaatcggtac tgggattaaa ctaagcaata aaaactaatg ttcccctcat tctttgaact
     2581 tctactattt gatttattta ttctgataaa tgacgatttc accttcagga tcttcaaatc
     2641 ccgtatagaa tttatttaag atctcttcag tatcagtaat aggataggcg ataggccaca
     2701 taccctttga tggttagaat aaactgctca aaatctgtat cgtatgttgg ctttagcata
     2761 ttaacaaagg ctatctccaa attataaacc ataagcgttg aatcgttctt aacaatttca
     2821 attcttccta acccaaagtc agaagatata attaccggac ttgccttacc cttcatgtgg
     2881 gttgttgaga ttccatattt aaagggaatt gtcggtacta tcggcggtct gaaaaacatc
     2941 ccaaaaataa aaaaatcttt cccctgctag tctcctaagt ccctttgtag cggatatggc
     3001 accataatct cattttatcg aagtgagtca tggtttatct gttggccaac caaaattgat
     3061 acaatctaac gatgcaccct ctacggcatt ctaggggagt gcacttgcat tcacccaata
     3121 aaaaccggca gttagcaaag aacttatgta gtcgtatgtt cttactgtta acatacaaga
     3181 ggtctccttt ccacaattgc ttagcagtaa acgggaattt acaaagctaa tctcttccat
     3241 tttcttatct ttgttctaaa agtcccaaat ggagcaactg tatttacatg aataaattta
     3301 tatacttccc atacagccgt tttggttgcc tcgtctgccc attttctttt gtgtgcagtg
     3361 aatagatctt catcactcat tgaatcaatc atcatgttaa tatcatcaat attacgattg
     3421 agaatttcct ctaattcttt caatggtaga tgtgcatagg tatttgtaaa ccacttatat
     3481 aactctccta actgattcca tttaaattga tcagaaggcg ttttaacaga taatccattt
     3541 ctttcatcat tttcccattt tagaattaaa ttcgtccaac caacttgata tgctaaattt
     3601 tcagcaggtg ttctatccac ttcctcaact cttttatcct tcaattcttc aggaatagtt
     3661 tcaaattcta aaatatattt agtataagtc ctatttattt ctttttttaa ttcttcttta
     3721 tttttatatg ttctcattat attacctcca taaattccta attgtctcct ttaattcatc
     3781 tataatttca tccacgatat tttaatccgt tctacaatgc atatctgata tgcattgact
     3841 gtctaactca tatgtgaatg atttataaaa tatcccaacg tctcatctaa agaaatgttg
     3901 atcgtcagca aggagctaaa aaatacgaat atatttcttt tgtagtaaaa tcggactggt
     3961 ttgcatatca ttttcttcgt aaatatcaaa atcatgttaa ctactatcaa ttactaataa
     4021 aaactaccac tttaagcgtt aagaactgtt aagtttagtt aagtgaggat agttcacaaa
     4081 atccattgtt aaacttcacg aaagtggtaa aactcaaaat gctttatata ggattacagc
     4141 atcgctcttt cttcgattgc gcgttgggta aaaaatatta tgaagtaaaa attgacgatg
     4201 acacgttcct tacggctcat cagatgaaac agctacaaaa acgaaatgat caactcgaag
     4261 aaaatttaat tctaaaaaag cgattgccat attcacgcct cgctcgacga actattaaag
     4321 gtcaagattt acctgaagat catccttttt aggaaattga agctttttat gttgcacaat
     4381 gcatttataa tactacaatg atgttttcac cagatattct tctagggtgc ggtgtaatga
     4441 aacagataca cttgcaaaag aaaattcacc gcgaatttac taaaattatg aataattatg
     4501 ttaatcttcc gaaatttttt tccaaaatta agggatcaag ctggtgttat agattgttta
     4561 aggttggcaa aaaaataact tctttttaaa caattataga aaaccgatac tctgttaagt
     4621 tggttgacac ggtgtcgatt tttatgactt tcttattctg aaacttcact aacggacagt
     4681 acctaaaaag tctatagtat ggacgtttac gagtactatt ttatcaaaaa tgctgctcat
     4741 ttcaacttat atgttttagt gaactacccc cacttagc
//
LOCUS       DACEKH010000132         4726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEKH010000132 DACEKH010000000
VERSION     DACEKH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.139"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            130..1275
                     /gene="tgt"
                     /locus_tag="IW662_002514"
     CDS             130..1275
                     /gene="tgt"
                     /locus_tag="IW662_002514"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ0867310.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1372..1752
                     /gene="yajC"
                     /locus_tag="IW662_002515"
     CDS             1372..1752
                     /gene="yajC"
                     /locus_tag="IW662_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ0867311.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            2057..4654
                     /gene="adhE"
                     /locus_tag="IW662_002516"
                     /gene_synonym="adhC"
     CDS             2057..4654
                     /gene="adhE"
                     /locus_tag="IW662_002516"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ0867312.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         1655 a          834 c         1010 g         1227 t
ORIGIN      
        1 ataaaaaaag gataaaaaaa agaatattaa ataaaataga aggaagccga gggaacttgc
       61 aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa gatattttat atagaaagta
      121 gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa tcaagaaaga aaaacatact
      181 ggcgcgcgtt taggggaatt gatcacgcca cacggaactt tccccacacc aatgttcatg
      241 ccagtaggaa cattagctac agtaaaaaca atgtccccag aagagttgaa agaaatgggt
      301 gctggcgtta ttttaagtaa tacatatcat ttatggctac gccctggtga agacttagta
      361 gaagaagctg gcggactgca taaatttatg aactgggatc aaccgatttt aacagattca
      421 ggcggctttc aagttttttc attaagtgat atgcggaata tcgaagaaga aggcgtgcat
      481 ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac cagaaaaagc aatcaacatc
      541 caaaataaat tgggctctga tatcatgatg agttttgacg aatgtccacc attcgatgaa
      601 agttacgact acgtgaaaaa atcagtagaa cgtacgtctc gatgggcaga acgaggattg
      661 aaagctcatg ctaacccaga ccgtcaagga ttatttggaa tcatccaagg agcaggattt
      721 gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga tggatttccc tggttactca
      781 atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga atcgtgtatt agaattcact
      841 acaccgttga tccccgaaaa caaaccacgt tacttgatgg gtgtcggtgc tgctgattca
      901 ttgatcgatg gtgttatccg tggagtagac atgtttgact gtgtcttgcc tactcgaatc
      961 gctcgaaatg gcacatgtat gacttctcaa ggtcgtctag tagtgaaaaa tgcgaagtat
     1021 gctcatgatt tccgtccaat tgatgagaaa tgtgattgtt atacatgtaa aaactataca
     1081 cgtgcctata tccgccattt gatcaaatgt gatgaaacat ttgggattcg tttgacttct
     1141 taccacaatt tatacttctt gttgaatcta atgaaacaag tacgtcaagc aatcatggat
     1201 gataatttgc ttgaatttag agaagccttt tttgaagaat acgggttcaa taaagaaaac
     1261 gcaaaaagct tctaaaggaa aatcttttaa aaattaagta aaaaactagt gttagcagca
     1321 tttttctgct aaagtgtttt ttgtagatac agaattagtg aggtgaaaaa aatgggaaca
     1381 ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt ggttttttat gtcacgttcg
     1441 caaaagaaac aacagcaaga acgtcaaaac ttgttagata gcatgaaacc aggtgattca
     1501 gtcgtaacga tcggcggatt acatggagtt gtttcagaaa tcgacaatga caaacggaca
     1561 gttgttttag actgcgaagg gatcttcctt gagtacgatc gtgcagcaat caaaacggtc
     1621 aaaccaggga ctgtcgtaac aaatgatgca acaactactg ttgaaacaac agaagaacca
     1681 gctgagacag ttgaagtaaa aactgaaaca acagaaacgc tggaagagac agaagagaag
     1741 aaagaaaaat aagtatcaaa gcttatttaa agacgaggta gggcagaaag atgtcctacc
     1801 tcatttttta ttcaaaaaag aaagagataa gagtatagga tatattgctc aatattgaat
     1861 aatcgggtgt gtcatataaa taataataaa taactttata tatttttcta tacaaacgcc
     1921 tataaataaa gcaactttat atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt
     1981 acatagaaaa ataaatattg tatgatatac atgtgaatga tgtaacaaat aaccatgacc
     2041 cattaggagg ataatgatgg ctaaagtaat ggaaaaagaa aaaacgaaga caattgacgt
     2101 acaagcaatg attgatgatt tggcagaaaa agcaaatgca gcattgaaag aaatggaaag
     2161 ctttgatcaa gaaaaagtgg accatatcgt gcatgaaatg gcaatggcag ctttagatca
     2221 acatatgcca ttagcgaaga tggctgtaga ggaaacaggc cgcggtgttt acgaagataa
     2281 agcaatcaaa aatatgtatg cgtccgaata tatttggcat aatatcaaac atgataaaac
     2341 agttggtgta atcaatgaag atgcccaaaa aggattgatt gaaattgctg aaccagtcgg
     2401 tgtggtttgc ggagtaactc caacaacgaa tccgacttcc acaacgattt tcaaatcaat
     2461 gatcgcgttg aaaacaagaa atccaatcgt ttttgctttt catccaagtg cccaaaaatc
     2521 ttctgcagaa gcagcacgag tagtacgtga tgctgctatc gctgccggag ctcctgaaaa
     2581 ttgtatccaa tggatcgaac atccttcgat cgaagcgact tctatgctaa tgaaccatcc
     2641 aggtatcgcg atcgtcttag cgacaggtgg agctggaatg gtcaagtctg cttactctac
     2701 aggaaaacca gctttaggtg taggtccagg taacgtacca gcttatattg aaaaaacagc
     2761 aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa acatttgata atggaatgat
     2821 ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc tatgcagcag taaaagcaga
     2881 attccaagca caccaagttt atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc
     2941 agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc gttggacatt ctgcgatgga
     3001 aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt
     3061 ggaaggcgta ggcccagatt atccgctatc aagagaaaaa ctttctccag ttcttgccat
     3121 gatcaaagct aacaacacgg accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg
     3181 aggattagga catacagcag ttatccattc agaaaacgaa gagttgcatg tcaaattcgg
     3241 tcttcgaatg aaagcttgtc gtattttagt gaatacacca tctgcagaag gcggcatcgg
     3301 tgatatctat aacgaaatga ttccgtcatt gactttggga tgcggttctt atggtaagaa
     3361 ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg
     3421 gagaaataac atgcaatggt tcaaattacc gccaaaaatc ttctttgaaa agaactctct
     3481 attgtactta gagaaaatgg aaaacgttga acgtgtcatg atcgtttgtg accctggtat
     3541 ggtccaattc ggttactgcg atacagttcg tgaagtattg gcacgacgta aaaatgatgt
     3601 gaagatcgaa gtcttctcag atgttgaacc gaacccatcg acaaataccg tatacgcagg
     3661 aacgaaactg atggcagact tcaagccaga tacagtaatc gctcttggag gtggttctgc
     3721 aatggacgca gctaagggaa tgtggatgtt ctatgaacac ccagacacat ctttctttgg
     3781 tgcaaaacaa aaattcttgg atatccgtaa acggacatat aagatcgaca aaccagaaaa
     3841 aacacaattc gtatgtatcc cgacaacatc agggactggt tctgaagtaa cgccatttgc
     3901 ggtcatcact gacagtgaaa cacatgtgaa atatccgtta gcagattatg cattgacgcc
     3961 agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt ccagcatctg tcacagcaga
     4021 taccgggatg gatgtgttaa cacatgcaat cgaatcctat gtttcagtca tggcttcaga
     4081 ttatacacgt ggattgagcc tacaagcaat caagttagtc tttgactatt tagaaaaatc
     4141 agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac aacgcttcta caatggctgg
     4201 tatggcgttt gctaacgcat tcctaggtat ttgtcactca gtcgcacata aaatcggcgg
     4261 ggaatacggg atccctcatg gacggacaaa tgcgattctt ttaccgcata ttatccgcta
     4321 taatgcaaaa gatccacaaa aacatgcgat gttccctaaa tatgactatt tccgtgcaga
     4381 tacagattac gcagatattg caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt
     4441 agtcgaagct ttagcaacgg ctgtttatga attaggaaaa tctgtcggca tcaacatgag
     4501 tctaaaagct caaggagtca cacaagaaac actagataca accgtagacc gtatggctga
     4561 attagcttac gaagaccaat gtacgacagc taatccgaaa gaaccattga tctctgaatt
     4621 gaaacagatc atcattgatg cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata
     4681 agaaaataga tgaaaaagaa cttggaaaac gttgtttctc caagtt
//
LOCUS       DACEKH010000133         4714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEKH010000133 DACEKH010000000
VERSION     DACEKH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.140"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(213..443)
                     /locus_tag="IW662_002517"
     CDS             complement(213..443)
                     /locus_tag="IW662_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ0867313.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(673..1491)
                     /locus_tag="IW662_002518"
     CDS             complement(673..1491)
                     /locus_tag="IW662_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0867314.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1652..2341
                     /locus_tag="IW662_002519"
     CDS             1652..2341
                     /locus_tag="IW662_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0867315.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2355..3857
                     /locus_tag="IW662_002520"
     CDS             2355..3857
                     /locus_tag="IW662_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0867316.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3870..4346
                     /locus_tag="IW662_002521"
     CDS             3870..4346
                     /locus_tag="IW662_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0867317.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4443..4553)
                     /locus_tag="IW662_002522"
                     /pseudo
     CDS             complement(4443..4553)
                     /locus_tag="IW662_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1590 a          673 c          830 g         1621 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      901 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      961 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1021 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1081 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1141 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1201 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1261 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1321 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1381 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1441 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1501 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1561 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1621 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1681 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     1741 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1801 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1861 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1921 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1981 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2041 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2101 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2161 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2221 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2281 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2341 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2401 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2461 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2521 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2581 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2641 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2701 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2761 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2821 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2881 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2941 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3001 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3061 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3121 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3181 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3241 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3301 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3361 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3421 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3481 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3541 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3601 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3661 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     3721 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3781 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3841 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3901 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3961 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4021 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4081 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4141 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4201 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4261 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4321 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4381 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4441 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa
     4501 aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt caaatataaa
     4561 atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac
     4621 ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac
     4681 tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DACEKH010000134         4662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEKH010000134 DACEKH010000000
VERSION     DACEKH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.141"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            255..530
                     /locus_tag="IW662_002523"
     CDS             255..530
                     /locus_tag="IW662_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867318.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            543..2621
                     /locus_tag="IW662_002524"
     CDS             543..2621
                     /locus_tag="IW662_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0867319.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2781..>4662
                     /locus_tag="IW662_002525"
     CDS             2781..>4662
                     /locus_tag="IW662_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0867320.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVE"
BASE COUNT         1553 a          746 c         1006 g         1357 t
ORIGIN      
        1 tttgtcgcag ccccattttt ttgttgtgat aaaagaacaa gaaaggcagg aatgggaaag
       61 taaacatcct tttgtcagaa acttttatct ttcttaagtt ttacttgata tctgatacta
      121 tttggaatag aatgaggcgt tgaaaaattt atggaaattt ctaactagca ataaacgatg
      181 aaacaatgaa atatttttca tccaaaaaga actttgctat aatgatggaa gtttgtgtat
      241 aggagatctg gaaaatgaga gagagttttg aacaacaaaa aaaactgttg catgatcgct
      301 atggcgccct ttcgatggat gaccgccgtc aaatactttg taagttgcgt aaaagaaata
      361 tattgatgta tcgtcagttg gaacgtctga aacatgatct gcttcgtttg gagtcaaaac
      421 gtgtacagtg tgaattagag gggaaccaaa cacaggttga agtagtcgaa accaaaattt
      481 taaagaaaaa agaacaattt ttaaaaatgc ttacacaaaa taaaaaataa aggggatgga
      541 cgatggaatt tgctgagtta gttattgtat ttgcagtaac gatcacctta tcgaacatag
      601 ctagccgcat tttgccaatg attccagctc cactgatcca aattttcctt ggagtggtat
      661 taggtctgac agagtgggga caatcaatcg attttgaacc tgaattgttc ttagttatga
      721 ttattgcacc attgttattt agagaaggag aaaaagcaga tatttcttcg attctcaaaa
      781 atttcgggac aattttgttc ttggcatttg gaggagtcat tttgaccctt gtcggggtag
      841 gtgcgacgtt atcattcctg ttgccaagtg tcccacttgc tgcctgcttt gcttttggag
      901 cagcgttagg tccgacagat gcggtagcag tcagttctct ttctggacga gtgaatatcc
      961 ctaaaaaggc gatgcatatt ttggaaggag aaggtctttt gaatgatgca tctggtgtta
     1021 ctgccttcca gtttgctctt ggtgcgctga tcaccggatc tttttcagca gtaaacgcgg
     1081 ggatgagtct agtagtttct agtattgggg gagctttgat cggctttttg cttgtctggt
     1141 tcaaacaaaa aataattcat ttgatcgaaa aagcatcagc acaagatgtc acagggtatc
     1201 tgctgatcga acttttgctt ccttttctag cctatgtatt ggcagaggca tttgatgtat
     1261 ctggaatcat tgctgcagtg gcagcaggta ttctccaagc ttctggtttt aggaaaatct
     1321 ctgtgtttga tgcagaattg tcgagcttgt cccacagtac atggacaact attgctttta
     1381 cactgaatgc attagtattt atatttttag gaatcgaatt gacgcaagtg ttctcgccag
     1441 tatggggaga cggtttatat ccaaacggtc ttttattagc gattatcgta ttaatctctg
     1501 tcatgctttt tgtgatcaga ttcatttcta tcagtctttt ttatgtattc aaagatggca
     1561 gtaaaaagtt caaaaaacag ttgaacgaga tcttgatctt aacatttggc ggtgtaaagg
     1621 gaacagtcag tttggctacg atttttattt tgccattttc aatcaataat atgatgtttt
     1681 accagcggtc tttgcttttg tttctgacgg cgggtgtcat cttagtgact ttggtcatag
     1741 ggatcattgt cttgcctatg ctgacggaaa cagaggaagc gaaaagcacg gatttgaatg
     1801 ctttgatgat tttagaagag gtcgttgaag ttttgcgaaa agaaataaag gaaattgatc
     1861 gtaatacaaa agaattttta gccactgaag cagtgataga aaattatcaa gaacggatac
     1921 gtgatctgta cttggaagat ttaaccgatg atgaaaagca agaagtacaa gaaatacaag
     1981 cactgatttt gtcgatcgaa cgggatggat tggatgaaag ttatcgttcg ggcaagcttt
     2041 cttctaatgg ttatcgtttt tactcccgtt ttctttctcg attcgaacat tctatcacaa
     2101 gccagatttt gtcttttatc ggcttttggt tcattgttgt ccgtaggtta atgcgaatcg
     2161 ttctccatcc gaaaatgttt tggcaaagaa gatatgctga tcgtcagaca tttattagtg
     2221 atgaagatat ccaagaaatc agagagacat atcaaaaaaa tactcagttg attattgaaa
     2281 gtttagataa cttgactgat atttatgatg aaacgatggt tcaatttttt atccagcaac
     2341 gcaaacttga aggaataaaa atgaactcgg ggaatttgat ttcttcgtgg atgatccagc
     2401 aagatagtct gtttacgaaa aaaatgctaa gaggttatta tcttgaaaga aaaatcattg
     2461 atgaatacga agttgctgaa aaaatcacga cgttttctgc gaacagctat cgcaggaata
     2521 tcaatttgtt agaatcttat acgatgaaca aaccatctga taacttttct ttccgatttg
     2581 cgtttcgagc aaagtcaaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga ag
//
LOCUS       DACEKH010000135         4606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEKH010000135 DACEKH010000000
VERSION     DACEKH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.142"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(373..1326)
                     /locus_tag="IW662_002526"
     CDS             complement(373..1326)
                     /locus_tag="IW662_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0867321.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1349..2104)
                     /locus_tag="IW662_002527"
     CDS             complement(1349..2104)
                     /locus_tag="IW662_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0867322.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2101..3060)
                     /locus_tag="IW662_002528"
     CDS             complement(2101..3060)
                     /locus_tag="IW662_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0867323.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3053..4003)
                     /locus_tag="IW662_002529"
     CDS             complement(3053..4003)
                     /locus_tag="IW662_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0867324.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4153..4605
                     /locus_tag="IW662_002530"
     CDS             4153..4605
                     /locus_tag="IW662_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ0867325.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
BASE COUNT         1502 a          968 c          784 g         1352 t
ORIGIN      
        1 tattgccgag aagcagcaga tggaacatcg tttaaagaag agatcggtac aaaataaaaa
       61 tatctaaatt taagttttat ctaagtttcg ctttgtacct ttgattcaaa tcaaaggagg
      121 cgatcttgat gctgaaaaaa tggatcacac tattttttgc cactccccat tctagaatca
      181 acccgtatga tgtagagaat gaattcacgt tgaaaaaaga tttcccaaat aaataaaaac
      241 catccgtaaa atcatcaata cgattttacg gatggttttt gataacttct ttgaggatag
      301 gctcttgcta tttaagaatg taagaaacat tattgttaca tagtatgaga gtttaatcct
      361 ctgttattta tgctatttaa atgcttgatt cacatcttcg atcatcagtt tcattgattc
      421 taaaccgccc ccgcttaaat accagacatc tggttcaagg gagatgatct gctgattttt
      481 accagcattc gtttgtgcta ccagttcatt ggcagaaata tcatctttgc tgtcgtctcc
      541 accaatagct tttgttctgt ctactacaaa taagatatcc ggattttttt ccagcacata
      601 ttcataagaa acactttgac cgtgagtgga tgcttcgatt tgatcatctg cttgttcaaa
      661 gccaaataaa tcatggatga aaccgaatct agaaccagat ccgtaggctg ataattgccc
      721 ttcattcaca agtgtgacta gtgctttttt gtcagtagct tctgctttct ttttcgtctg
      781 atcgattgca ctagataatt cctctaattg agttttagct tcttcttgct taccaaagat
      841 ctcccctaaa gcagtgacat tttgctggaa agattcccat ggatttttcg catctaaaga
      901 aaggaaaatg gtcggagcaa tttgacttag atctttttga aaatctcttt gccggccaga
      961 aatgatgatc aggtctggct gaagctgatt gattttttct aagtctggtt ctttgatccc
     1021 acctgctgat tccacttttt tataggatga aagatactca ggaaggctgc ttgtagctgc
     1081 tccaactacc ttatctccaa caccaagagc atccatcgta tctaaagaac cattgtcgaa
     1141 tacgacgact ttttcaggat tctttggcac ctcgatttga tcgccattac tgtcttttat
     1201 cgttaatgcc gtttcagctt gcgtactgct tttctttgta tctgcctgac tggtgttgtc
     1261 actatttcca catgctgcta aaccaaatga tgctagaatc aagataccta ctactaattt
     1321 tttcatctct tctctcctaa tgttagtttt agaaataaag acagaatctg cgaccttcaa
     1381 tctcacaaat tcgtatgtcc atttcgtaca attcgtccag tacatctttt tttatcattt
     1441 cttgggtcgt tccttgcgtg aataactgcc catctttcat tgcaataatt tcgtctgcat
     1501 aacttgcagc aaagttgata tcatgaagaa caataacaat tgttttcccg atttcttgta
     1561 ctaatttttt taagatctgc atgatttgaa ccgcaaaatt catatctaga ttgttcaaag
     1621 gttcatccag taaaatataa tccgtgtctt gcgctagcac cattgcaata tacgcacgtt
     1681 ggagttgacc gcctgaaaga gtgtcaatcg attgatccgc tatttcttct aacccaaggt
     1741 gttccagtga ttcctgaacg atttcctcgt cttttgtcgt caattttcct ttcgtataag
     1801 gaaatcgtcc aaattctact aattctttca cggtgatttg gacatttatt tcattcgatt
     1861 gtcgtaggat cgataatttt ttggctaatt cattggattt ccaagccttt acttcgttat
     1921 ggtctaaata gatttcacct gtatcttttg gtatcaaacg actgatcatc gacaacaccg
     1981 ttgactttcc agcaccattt ggaccgataa aagcagtcaa cttcttttca ggaatcgtta
     2041 actgtacatc tttaaccact gattttccat ggtacagttt attgaccttt tgtaaatcaa
     2101 tcataggata ctcttccttt cgcgaatcaa ttttgtgaca aaataaattc ctccgacaaa
     2161 ttcaacgacg acactcactg ttgtattcca gccgaaaatc tgctctactg cgagctgtcc
     2221 tcctgtcaga aacagcatag ctaccagaaa gcttccgata aatagctgac catgacgata
     2281 agtagaaaag acacgataac tgatatttgc gacaataaac cctaaaaaag taactggacc
     2341 cacaagagcc gttgatgtcc ctgtcaaaag actgacacaa cataaaatca aaaattcaaa
     2401 acgatctgtg tctatcccga ggtttactgc ttgttcattt ccgagatgga aaacgtctag
     2461 cacatgtcga tatcgaaata acaggtacca gcagccgata atgatcaaag aagcaggcaa
     2521 aagatactga ctctggatat tgccaaagct tgcaaataat ctgccttgaa gcaaatcata
     2581 ttcattcgga tccatgatga cttgtaaaaa ggtactgaca ctaccaaaaa gcgtacctaa
     2641 aatcatccct gacatcagta ataaaaaaag attcgctctc atttttttca aaagaaagaa
     2701 aaataacaat agactcagcc cgaccatcag cccaacgttc aataagaaaa aaactgtacc
     2761 tgtctgctga agcatcgtca ctcctccgat aaagaagaaa aggaccgttt ggacaaggac
     2821 atataacgac tcaagtccca atactgaagg agtcaaaaac tggttatgcg taattgtctg
     2881 aaagctgatt gtggcactgc tgacagctag cgagacaagg ataaatgcaa aaattttctt
     2941 actcctcaat gcccatgcaa aatgccagtt tccatatgta tggatcgtca agtacattcc
     3001 tgtaacaaat aaaacaagaa caagcatacc aataaaaata gatggctttt ttttaagcat
     3061 atcgtcgccc ccttatcaga agataaagga agatagcccc accgatgatt ccaacgatca
     3121 cactgactgg tatttcataa ggaaaaatca acacgcgagc gacaagatcg caaaatacta
     3181 agaacaaact acctgctaaa gcagttacgc caattgtttt gtgcacttga tctccatata
     3241 ttctagagac aagattcgga atgatcactc cgataaaagg aatgcttcct accatgatca
     3301 acatgactgc actagccaat gatactaagc caatgcctaa aaactgcatc ttctggtaat
     3361 ctattcccaa atttactgca aacgattcgc ccagaccaat gatcgtaaat cggtaagcaa
     3421 atagccaaac caagagcaac aatggaacaa cagcatacaa taactcataa tttcctcgta
     3481 tcacagtcgc aaaatttcct tgaagccatg aagtcatatt ttgtaccaac tggaattgat
     3541 aagcaaaaaa agtagcaact gaaccaatga tattccctaa catgactcca atcaacggaa
     3601 gcatcaattg atttttctgg gggaacaatc gcgtaaggga taaaaaaagc aatgttcctg
     3661 caaaagcaaa gcaaaatgct gtcaaggatc ggacaatcaa ggatgcacca ggtaggaaaa
     3721 tcatagccac taaaatacct aaacgcgcac tatccatcgt gcccgcagtc gttggtgaaa
     3781 caaatttatt ttgggtcaaa tgttgcatga tcaacccaca aatactactg gtcgctccca
     3841 caaggatcaa acaaatcgtt cgtggcaaac gagtcactag caagatttgt aagctttgcc
     3901 catctaactg gagcagttct gacaattgta cattcgatac tccgatgaac aatgaaatac
     3961 tacttaaaat aactaataat gctactagtc gtccgtagcg catccaatcc acctttctaa
     4021 ttgagaatga ttttcattct tattttaagt gaatcgttga acaaaagcaa ccgctttttc
     4081 atatgtttta ggtattattg ggaatatgct ataatcgtaa gaaagacaaa gagaagaagg
     4141 agggtatttt ttatggcaca agatgatgta aaaagccatt tggataaggg actttatggt
     4201 acacctttag tgaaccctga agaacagcat aaatatttag ggacattcag ggaacgctgc
     4261 tatcttagta tgactgttgc tgaaatgatc caaccaaaaa acaaagaaaa ctttttaaaa
     4321 gaactcaaaa aacatccaga tgcttccata ctcttgaatg gggctctacc cctttctgtc
     4381 caaaaccaat atatccagct agcaacgaaa aacaatagcc gatttactgt agtcaacgat
     4441 tacgtcactg caaatcctga ggaattcgga ttgctcttaa cagcaaaaga agcagtaaat
     4501 gaacctgtga ttgatatcga aaaaaaatac ccagaagaaa actcttcgcc agttgaaaaa
     4561 gaaaaaccaa aaaagaaaaa aagcttctgg gatcgtttat tttaaa
//
LOCUS       DACEKH010000136         4464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEKH010000136 DACEKH010000000
VERSION     DACEKH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.143"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            272..973
                     /locus_tag="IW662_002531"
     CDS             272..973
                     /locus_tag="IW662_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0867326.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1582..2598)
                     /gene="tsaD"
                     /locus_tag="IW662_002532"
     CDS             complement(1582..2598)
                     /gene="tsaD"
                     /locus_tag="IW662_002532"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ0867327.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2595..3059)
                     /gene="rimI"
                     /locus_tag="IW662_002533"
     CDS             complement(2595..3059)
                     /gene="rimI"
                     /locus_tag="IW662_002533"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0867328.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(3066..3608)
                     /gene="rimI"
                     /locus_tag="IW662_002534"
     CDS             complement(3066..3608)
                     /gene="rimI"
                     /locus_tag="IW662_002534"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0867329.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3592..4416)
                     /gene="tsaB"
                     /locus_tag="IW662_002535"
     CDS             complement(3592..4416)
                     /gene="tsaB"
                     /locus_tag="IW662_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ0867330.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1353 a          922 c          750 g         1439 t
ORIGIN      
        1 tttattcatt gaaaaaattt ttttgaataa attaaaaaaa taaattatat tatcgttgtt
       61 agcgtttact atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa
      121 ttattcaata ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa
      181 acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa
      241 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
      301 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
      361 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
      421 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
      481 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
      541 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
      601 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
      661 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
      721 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
      781 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
      841 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
      901 cgggctgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
      961 tatgagagaa taacgcgaac aaaaataagc agtgaccctg ataaatatca gggtcactgc
     1021 ttattttcct ccctaaaact tctgaaagat ccggcagttt tagatcactg gacaagactc
     1081 aaagattttc tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgtta
     1141 gattaactag atgaagcgtc ttgtcctcat ccgatctaga ttattcgata ttcctcgaca
     1201 ataaacgatt actcagtgag ggcaatcaga ggaacggata atgataaata agctgtaaaa
     1261 aatagcaaaa taacaataat gattaaaagt atgtaaaaaa tagggctttg acaaaaacgt
     1321 gtcacagccc tatttcgatt aaacggtgtg tacctgtcaa ctcttcggtc acaagtcata
     1381 atgttcataa acatgaggag ctgttttttg aaattgttcc ttgtgaatca gagttaaacg
     1441 acaggcttac gaactcttga ttaaacggcg ttcaaaagct tgcatacgcg gtaataagaa
     1501 cagccaggtc aaaaacatga gaagccgttt ttcctgggct gttcttatta cttgatgcag
     1561 ggcagctttt tcacaacctc tttattcaga taaaaccaag cttggatcag cattcaactg
     1621 atagctagca aaatttcttt tttgcatttc cacatgtgct gctgctccaa tcattgcagc
     1681 attatctcca caaagtcgaa gagggggaat cactagttct acctcaggga gtttagttgc
     1741 caaagcatcc tgcaatcctt cacgcaaccc ttgattggcc gctacacctc cagctataat
     1801 cagttgcttg actggataat tttgacaagc tcgaagtgtt ttagtaatca atacatcgat
     1861 cacacttgct tgaaagctag ctgctaaatc atttttgtct agatcttctc cacgctgttg
     1921 tgcattatgc accaaattga taaatgcact ttttaatccg ctaaaactga aatcataatt
     1981 atcttcatgg atcatcgctc gcggaaaatg gtaattatct tttccctgat gagctagctg
     2041 atcgatttct tttccgcttg ggtaagaaag acctaatact cttccaactt tgtcataagc
     2101 ttctcctgct gcgtcatctc ttgtctcccc aatgatttca tatgatccat cttcttgcat
     2161 atagactaat tctgtatgtc ctccgctaac aagaagtgcc atcaatggaa actggaatgg
     2221 tttaaccaga cgggctgcat agatatgccc tgccatatgg tttacaggaa taagcggcaa
     2281 ttggtgtgcc caagcaaaag ccttagctgc agaaatacca atcagcaacg agccgacaag
     2341 tcctggtcca taagtgacag cgactgccga taaatcttct gccgacacac cagcttctac
     2401 cagtgcatct tccaagcata aggtgatttg ttctacgtga tggcggctag ctacttcagg
     2461 tactacgcca ccaaaacgtt tatgactgtt tatttgagaa gcaacgatgt tagataagat
     2521 ctctgttcca tttctaacga ccgctacact cgtttcatca caacttgatt caatcgccag
     2581 aatcaattct tcattcattt catcgtctcc gtttttaact ttctgctcat aatgatcgca
     2641 tcttccgtcg gattgcgata atatgatttc cgtttaccta atatacgaaa cttttctgat
     2701 tcataaagtt ttcttgctgc ttcattagat agacgtactt ctaaaaaaac atgcagcgaa
     2761 tcttttattt gttccgtaat cagtaattgt aacaaatgcc gtgcatgccc ttttccttgt
     2821 tctgaaactg caacagcaat atttgtgatc tcgacttcat ctattacttt cgagtatcca
     2881 ataaagccac agatctggtt atcttcagct aaaagcagat aatcagagtg aggctgttgg
     2941 atgtctgcca aaaactgcat tttcgtccaa ggtgaaccat gtggataagc attttcgcta
     3001 atcatccata cgcattctgc caagtacgct tcatcaaaat ctttttttct gcaaatcaac
     3061 taatctcact cattttctaa aagttttatc atatccaacg catcttcttt tgtttcagtg
     3121 taatatcttt ttttgatcgt tgttgcttga aaccccaatt tccgataaag tctttgtgca
     3181 tcttgattac tcatacgcac ttctaatgac atcgtctcac agcgattcat tatagcaaag
     3241 ttctcgattt cgtcaataag cagcgaaccg attcgtttac gctgatatgc cggtcgaacg
     3301 gcaatattcg tgatatggca atctaaaccg aacgctcttg aaccgataaa accaatcatt
     3361 cttccattat caaagatacc tagatataga tgaggaagcg gtgacttcaa ttctgatata
     3421 aaggcacttt ttgtccatgg caattcgcca ttatagactt ctcgttcgac atccagcagc
     3481 tctttgatat cttctgtagt caattctctt agtacgtaac gacgatcgat ttgttcgact
     3541 gttttttctg tataaatcat tttatcttga aaaaagcttc tggcatacgc tctaaatttt
     3601 ttcaacataa ctttcgtctc caggtgtgtg cgtctccaac catttttctt ctgcttctac
     3661 tcgttttaaa tagcgaggaa gaaaagcttg tttctctgca actggttcaa ctaaagcacc
     3721 taattgagcc acagttacac cacttggaat atcccatgct ggaactgtat taatcatcgc
     3781 actaggcaat atttccttta tttcagagaa gaatttgtga caatcactcc cgacgaaata
     3841 aatagcttca ttcagctttt gtagttgtgt gaacaaatca gaagcagaaa tatgtacatc
     3901 ttcaagtaca gtttctaatc tattttcttt ccagcggtat gccccagcgt atacattttt
     3961 tcttcgagca tcaaacatgg gcacaataat attatccaca ccgatgcaat tagccgcgat
     4021 ggttttcaga ctagatactc ctactagctc tttatctaat gtataagcaa tcgtttttgc
     4081 tgtcgttaca cctatacgaa gccctgtata agaaccggga ccatcagaaa ccgcgattcg
     4141 gtcaatgtct tttggactta tttgaagatc tgcaaacaat tggtcgattg caggcatcag
     4201 tgttacactg tggttgcgtt tgatatttgt ttgtatttgt ccaagaattt gttcgtcttc
     4261 taccacaccg atcgccaaag tttggtttgc agtatctatt cctaatgtaa tcatatattt
     4321 gatcctttct gctatgacat tctcgccttc cattgtagca tactttttta ttctgaggat
     4381 gaaaatacca aaggcaaaac tagtacaata atacatgatt atctgctggc gggcaaattt
     4441 tgccccttcc tgaaacacaa aaaa
//
LOCUS       DACEKH010000137         4374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEKH010000137 DACEKH010000000
VERSION     DACEKH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.144"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(83..1051)
                     /locus_tag="IW662_002536"
     CDS             complement(83..1051)
                     /locus_tag="IW662_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0867331.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="IW662_002537"
     CDS             complement(1498..2904)
                     /locus_tag="IW662_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ0867332.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="IW662_002538"
     CDS             complement(3126..3488)
                     /locus_tag="IW662_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867333.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3800)
                     /locus_tag="IW662_002539"
     CDS             complement(3492..3800)
                     /locus_tag="IW662_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867334.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3809..4228)
                     /locus_tag="IW662_002540"
     CDS             complement(3809..4228)
                     /locus_tag="IW662_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867335.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="IW662_002541"
     CDS             complement(4221..4361)
                     /locus_tag="IW662_002541"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867336.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1234 a          994 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 attgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACEKH010000138         4219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEKH010000138 DACEKH010000000
VERSION     DACEKH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.145"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            627..3266
                     /locus_tag="IW662_002542"
     CDS             627..3266
                     /locus_tag="IW662_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0867337.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3434..4132)
                     /locus_tag="IW662_002543"
     CDS             complement(3434..4132)
                     /locus_tag="IW662_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0867338.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1278 a          806 c          911 g         1224 t
ORIGIN      
        1 ttcttctttc tgttctgtcc aaattattct aagatttctc tttaccaaca tcatttgaca
       61 aagagccaga aaaagccgag ctctcgataa gctcggcttt cttgtacctc ggaaggattc
      121 aaataaacag aaatgattgt aactttccat catacatgca aagcacggtg ttgaatttct
      181 tcatgttaaa caaaaatttt gttttcgtta catcttcaac atttgaaaca tctcttgttc
      241 ctattcaatt ttcagacgtt tacgttattc aattgttacg tatcgcatta cttcttgttt
      301 cggatacttc gtgatccaaa catccatacg cattcactat ttataggttt gtactctaca
      361 cgcatgtctg ggtgaaacct gtacttctaa caactttatt tgcttacact gttctatcga
      421 gaaattcttc tgtctatttg atggtatcct ccttccttac atctatataa tagcatcctt
      481 tggtagcgtt tgcaaatata agcactttaa attttacgct ttcttaacaa ataatcgata
      541 agccctttat ttgaagtaaa taaaaaaagg atgttatagt aaaataagta cctttttttg
      601 tacgaagatt ttaaaggagg aataggatgg aagaatacaa gcagacaata gaagaagtaa
      661 agaaggtaac agaagcagat ccagatactg ggttgtcttc acaagaagtg gatgaacggc
      721 gaaaaaagca gggattgaat aaattcgatg aggcgccgaa agaatcgatg atcaaaaaat
      781 tctttcgaag cttatccgat tttacaacga tcatccttct tgtagcagcg gtgatttcct
      841 tttataccgc atttgctaca gaacatgggg atttatttga aggactgctg atcatcgcta
      901 tcgttgtgat caactcggta ttggcgattg tccaagaagg aaacgcagaa aaagcactgg
      961 aatccttgca agatatgaat aaacaaacgg caactgtcat acgagacggc aaagtgacca
     1021 ctgttgagtc agagcaactg gtagtgggtg atattcttgt tttagagtcc ggtgatgcta
     1081 tttctgccga tgcccgtttg atcgaagcat cacaattacg tgtagaagaa tcggcgttga
     1141 ctggagaaag tgaagcagtt gaaaaagatg cagcatttat tgctgaagaa gatgagtcat
     1201 taggtgacca acttaatatg gtatttaaag gatgtacggt tgctgcaggt cgtgggaaag
     1261 cgattgtcac tgccattggt atggctacag aaatgggtaa gatcgcggat cttttgaacg
     1321 aaaatactat gcaaaaaaca cctttacagg tccgattgaa tcagctaggc aaacgaatca
     1381 gtatgatcgc tttagcggct gcggctttag tatttgtgat tggtgaactg caaggagaac
     1441 cactccttga gatgttcatg acttcgattt ccttggctgt tgcagctgta ccggaaacct
     1501 tgactgttat tgttacgttg acacttgcat acggtgtgca gaagatggcg aggaaacatg
     1561 cgatcatccg acagttgcca gcagttgaaa cgctaggaac cgctaatgtt atctgctcag
     1621 acaaaacagg aacgctgaca caaaatgaaa tgcgcgtccg ccgggtatgg agcaaagaag
     1681 atgaagtaac gaatatcgaa gattcaatga cgaacagcgc gatggaaata ttgaaaatgg
     1741 ctgcactatg tacagatgtg acggtcgagc aagaagatga cgaccttgtt atcaaaggta
     1801 atccaacaga agtagccatc gtaagggctg ttgaagagaa ttatcataca aaagcagaat
     1861 tagaagaaaa atatccacga gtaaacgagc ttccttttga ttctgaacga aaaatgatga
     1921 caacagttca ccaaatgggc gaaaaataca tctcaatcac caaaggagca tttgatgttc
     1981 ttgctccgcg ctttagttca ggcgatgtag aacaagcggc gatcgttaat gatcgctttg
     2041 gtaaacgggc actgagagtg attgctgttg gttatgctac ttatgacgaa gaaccgcaag
     2101 atatttcttc tgaagcactg gaaaaagact tacgtttgat tggtctgatc ggaatgatcg
     2161 atccaccacg tccagaaagt aaaggagcaa tcaagcgagc gaaaaaggct ggcatcaaaa
     2221 cagtcatgat cactggagat catgtggtaa ctgccagtgc catagcgaaa gaattaggta
     2281 tcttgaatga tccgtcagaa gcactctcag gatcagaact gcatcaatta tcagacgaag
     2341 aattagatgc gcgtgtcaaa gctctctctg tctatgctcg ggtaacgcca gaagacaaga
     2401 tccggatcgt caaatcttgg caacgtacgg gtgcagtcgt agcgatgact ggggatggtg
     2461 tgaatgatgc accagcgttg aaagcaagta acgttgggtg tgccatgggg atcacaggga
     2521 cagatgtggc gcaaagtgcc gcagatatga ttttgacgga tgacaacttt gcgacgatcg
     2581 tagatgctgt tagtcaaggg cgttcagttt atcagaatat tcgtaaagca atcaactttt
     2641 tactaagctg taatatttcg gagatcttta ttgttttgat tgcgatgctg ttaggatggg
     2701 gagcaccatt tacagctgtg caactactgt ttgtcaatgt agttgctgat ggacttccag
     2761 gatttgcttt aggaagagag ccagcggaac acggcatcat ggatcagcca cccattccga
     2821 aaaatgaagg gatctttgct cgaggtttat tgcaaaaaat tgccataaat gcaggggtct
     2881 tcaccatcgt aaccttgttt ggttattatt taggtagtta tgtcgatact atcagcccat
     2941 gggtagacgc aagtcagcat gtcggtcaaa ctgttgcatt cttgatctta gcttattcat
     3001 cgattctgca tgtgttcaat gtacgcagca gccaatcgat cttcaaagtc aatttagcaa
     3061 ccaataaagc tttggtagaa atggcgttac tggcattagc tattacaacg gctgttgctt
     3121 tgctgccatt tacgcaagaa ctattcggcc tagtacacat cagtttgaat cactggttct
     3181 tggtagggat tttatctatc gtaccaatcg cggtgaatga actgatcaag ttccatcgat
     3241 taccggaagc agaagaagag gaatgattaa ctgtttctaa gttgattttc tttttactgg
     3301 aactatacaa aaaggaggtg tgacatgaat gttgtcacac ctcctttttg ctaatgggct
     3361 gaatgtttct tttactgcct ctcgataaac ggtgttctcc taccaacccc tcggtcacaa
     3421 gtcacactct cttttaaata tcttttggca attgttcata aaatgctgct tttgttgcgg
     3481 aaatgtatgg gctgagccat agataaccaa taccagcagt tgctaatgct aagatatgcc
     3541 agccgataaa tgaaagatct aaccagaaaa gttgtccttt ataaccatcc atcaattttc
     3601 gacttgcagt gatcgtatcc agtactttag gtgtttgtcc tgtttcttgg acgatatcgt
     3661 agtagataaa atagctttga gaataagaat agcttttaat gattccagga ataactaata
     3721 gcaaacccca taatgttgta aagatcgaga tcagcacagc taagccgatc actccgccga
     3781 taaacaaacc tttgaatcca cgaaatgcat ctttgaatgg ttcgatctga tctttttgtc
     3841 cacgtaaaat atcaagataa gtccaagaaa taccgctcat gaacaaagcg ctaatgatgc
     3901 tggaaataat tccgccaccg cctcctgaac taccgcctcc tgaagttgtc gcatctgaga
     3961 cgctcgctga tccatcatat atggtggtgg caaataagat aataggaatc ataataagaa
     4021 gaatagcagc aataccaatc aatgtaggga ttagatttag taaaacagct tgtccccaac
     4081 gtccttttag actctctttt gcttggcgtt ttaattctgc agaagattgc atgatttttc
     4141 cctccttttc tttaataaca ccaatatacc atgtatcaaa aaatatttct gtcataatgt
     4201 ttttctcttt tttgttatt
//
LOCUS       DACEKH010000139         4200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEKH010000139 DACEKH010000000
VERSION     DACEKH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.146"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..1223)
                     /locus_tag="IW662_002544"
     CDS             complement(3..1223)
                     /locus_tag="IW662_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0867339.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1243..1848)
                     /locus_tag="IW662_002545"
     CDS             complement(1243..1848)
                     /locus_tag="IW662_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0867340.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1897..2829)
                     /locus_tag="IW662_002546"
     CDS             complement(1897..2829)
                     /locus_tag="IW662_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0867341.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3254..4111)
                     /locus_tag="IW662_002547"
     CDS             complement(3254..4111)
                     /locus_tag="IW662_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0867342.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKNDTID"
BASE COUNT         1191 a          849 c          692 g         1468 t
ORIGIN      
        1 ttttatttta cagaagcact cgaaactgca tctactgcag tttcatcgtg tgtgctggcg
       61 ctggttactg catctgtttt tccaccatga acagatgcac ttgaaaccgc atcttcattt
      121 ccaacaagtt gttgacccgt ttgttgtaaa taccactcaa tgattggttt gaaatcaaga
      181 atgtattcat tgattccagc atactctcca ttttcatcat gcattgcttg gtaattatgc
      241 acaatatatt tttctggcac ttgttttggt acatgaacac gtaccgcatc tgttgctccg
      301 cttcgcagtt gttgaattac ccactctaca tttttatacg atttttctgg gtgacagttg
      361 gctaatggat tccctacctg accgggcaca cgagcagcta acatctcttt cgcttcttgg
      421 cgcaaattat aatataaaaa ttggttatta ttatctgcaa aagttaattc catcggcatc
      481 gaatctaaaa agtaattgat ttgatccact gtcaataacc cacgatttaa tttgacgtaa
      541 gtatttccag aaacggcacc gactgcttca gaagcttttt ccaaccaatg atcatctgac
      601 atatcaacat cgggtattgt cgtagcaatt gttcctgtcg catgcaaatc ttctggtgga
      661 agctttttag gccctgctaa ggtagacacg actttaatga attttacaaa acttgctaat
      721 gtactgccta aaaagtcaac agttcgtgga tcaatcaagc gatcttcttg gtcaaatgct
      781 tcttttgctt ttcccaataa aaattcattt ccgggcatca ctaatgcatt cacgcctggt
      841 gcgtccaaaa tttggcgcaa gtgtaattgc gcgcgtgaag ttccttgatc gtagtaagaa
      901 gcacccacaa ccattacagg tttattttca agtggatgga ttttaaatga tagccattca
      961 agtgcacttt tcaatcctgc tggaaccgta tgattgtgtt ctggcgtagc aataattacc
     1021 ccatcagcca ttaagatttt gcgatttagc tgttgaatag caggactatt tgtttgatca
     1081 tcatcttgat tgaacagagg taaatctttg atttcaatga tttctaaatt aaatagttca
     1141 tgatattgtt tttggataaa tgttaataat ttacggttat atgaaagttc agctgctgat
     1201 ccgacgatac ctattaattt catctttgtt tgctcccctc gattaaatcg tttcccatga
     1261 aaagctttcg gcttcacgtt tatttgcttg gtgcgcatgt tgcaattgac tagtgattgt
     1321 gacaaagagt aagaaatctt tgaatagttc acgtaatttt gcgacttgtt cttcatcaat
     1381 gagtcgtcct tgctcatcaa aggcttggag tgaatgaccc aataaaaatt ctgagcttgg
     1441 cataattcgt gcctttaact ctggtgaatc caatatttgg cgcaaatgtg cttgtgcgcg
     1501 tgaagaacct aacgtgccat aagacgctcc ggtaatcatc actggtttat ccacaaacgg
     1561 aaaaatacca tatgaaagcc aatttaatgc gttaatcaat gaagcaggaa ccgcatggtc
     1621 atactctggt gtgctgatga ttacgccatc tgcttgttca atttttccag caatttcttt
     1681 aacaacattt ggtagttctg tgttttcagg tttgttaaat agtggcagct ccttgatttc
     1741 gacaatttcg atttctgcct catttacaaa ataatttttc ataaactgta ataattcacg
     1801 gtttgttgac cgcgttgaat ttgttcctac taatccaata aatttcatct tcattctctt
     1861 tcctttctaa catcgatttc tgactgttcg cacgtactaa attttttcaa atccttttgt
     1921 atacaagaat tgattctctt ttgttacaac tgctccctcg atacccggct gttcttcaag
     1981 cgctttcaac acttctaaag aagatgctcc aaataaacgt gtagtccaga tttctccatc
     2041 tacagaagaa tcagacaaaa tcgtcaggct tgctacgtca tttttaactg gaaagccagt
     2101 tttcggatca atgatgtgat gatacactga cgaaccattt tgtagataac gttcgtagat
     2161 tccagaggta actaaagatt ggttgcttag tttaaccacc gcaagcgact cttcacgtga
     2221 acctttagga tttcgcaaac caatacgcca agcaccatcc gaacgtcttg gtgattcacc
     2281 aaaaacaacg atatttcctc ctaagttaac taaagcaaac gctgcatttc gctctcggca
     2341 atgtgtaatt aatttatctg caatgtaacc ttttgctaac gcccccaagt caatcatcat
     2401 tcctggtttg gttaaaaaaa cggttttcat tttttcatct aattgaattt ggtgtggatc
     2461 cgttagcaga agtaactcag ttatctctgc ttttgttgga accaatgcat cttcaaatcc
     2521 aatgtgccat ttttgtacaa ccggaccaat agcaatattc agataactat ttcttgcaca
     2581 actatgtgct tttcctaatt tgatgagctc aaaaagttca ggatgtacaa tcacagcttg
     2641 tcgtccagca gcttgattga tggccattaa ttcagaagtt gcatcatgtg cgctgaagcg
     2701 atgttcataa acgtgcaacc attcgcctaa ctctgctagt aatcgactag gctcttcatg
     2761 cccaacttgt aatgtaattg ttgtccccat catttgccag attttcgttt cttcgatcac
     2821 ctgattcatt ttgtaccttc tttacttgaa ctacggatta catgagtaat tagtttttat
     2881 ttttgttact gttttcacaa ccagatgata cgcctgctag atttatttgt caaccgattt
     2941 tatatatttt tttatataat aaggtaattt taatataaaa ttttatattg taaaaaaaca
     3001 aataagggaa acaaacaaca catttttgtg tgttgcttat ttccctctaa ctatcgtttt
     3061 ctatttgatg gttaacctac ttaaaataaa tactgcaacg acaaacggag atcaagcatg
     3121 ctttgtaacg tattttacga gaattacgac tttattttat tactctatct tgctgccata
     3181 cccgtctatc cctaatcatc tttggtgaat tttttcaaag ttagcataac aaaaataagt
     3241 attttttcct tcgttaatca atagtatcat tttttttcat atgatttaat tcatcaatta
     3301 attttttgtt tttattaatt ttagcaaaag ctagtgcatc ttgaatcaat tcgagcactt
     3361 ctgccttcga cttattttta gcctgtgcgt tcttcgctaa ttgggcagca gctcgtgcta
     3421 agtaataagt caaatggtta tccgaacaaa tatcgattgc gtaatttagt agtgcatcac
     3481 ttgtttccaa atcaccgtct tgcgcaaaaa aaacaccaca atgaaagacg atatttaaca
     3541 cccgccagat atcttctgtt gtacgaatcg gatacgtata aattttttca aacactcgat
     3601 tgaaataaac ttctgctttt tccaactctt tttctctagc atagaccatt ccaatacctg
     3661 tatatgccaa taaacgaaac aacgtgttgg ctgtttctgc catcaacaac tgatcaaaat
     3721 ggaacaacac atccgtaatt ggtgcattcg taaaaatcat ttcaaatcct tttagatagt
     3781 agtaacgtgc aatcagctca gactcgtcaa ttaccttggg atcaatggct gctaaaatct
     3841 gtgtcaattg ttcatattca ctcgtaatca gtgaaaactc tgctttttct agctgttcat
     3901 caagttgtgt attgatcacg ttcatcgaag aaaaaagttc aactaacgga agattgattc
     3961 gattacataa tttgattaaa atttttaacg aagggacttg accattgttc tcaaatttac
     4021 ttaatgttgc ttgtgtacaa attccttcag acacttcttt ttgagataag ttggcagcct
     4081 ttcttgccgt aataaatttt tcaatgttca ttattttctc tccaatcaga cctcacacga
     4141 aaatcatttc tgtctcgatt ttagcatgtt ttctttggga atgctttaat tggttaagat
//
LOCUS       DACEKH010000140         4107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEKH010000140 DACEKH010000000
VERSION     DACEKH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.147"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(217..1722)
                     /locus_tag="IW662_002548"
     CDS             complement(217..1722)
                     /locus_tag="IW662_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0867343.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1719..2405)
                     /locus_tag="IW662_002549"
     CDS             complement(1719..2405)
                     /locus_tag="IW662_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0867344.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2598..4019)
                     /gene="gndA"
                     /locus_tag="IW662_002550"
     CDS             complement(2598..4019)
                     /gene="gndA"
                     /locus_tag="IW662_002550"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0867345.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1132 a          864 c          695 g         1416 t
ORIGIN      
        1 aattccctat ttttatttat tatttttaaa cacgaatatt acagcatttt attgtttttt
       61 cgttcatcta aaagatactt acatttaatt aacggatgtt attctattta attgttcgct
      121 agttttttta taatgacatc cctgaatttc tcacttgctt gtattttgta ccttattctt
      181 tgttggatat ataaaaaaac aagtaaagca catgctttac ttgtcttctg cctttgcgat
      241 aggaataaag attctaaaaa ttgtcccttg ccctactaca ctttctgcta agatacgtcc
      301 tttatagttt tcaaccagtt gtttagcgat agacaacccc agaccatttc cacccttcgt
      361 tctagctctt gctttatcca ctcgataaaa acgattgaag atcttattaa ggtcttcttt
      421 gggtattcct tcgccaaagt cctgaatcgc gatctcaaat tcattcagag tggaagaaat
      481 agagatatgg acttcttttc gtgtagttga atactttact gcgttatcca atatgataat
      541 gattaattgt tcaaagtgat tacgataaat ttgcagggtc acttcttttg tcaaatcatc
      601 atctaaagtg atcacatagt ctggatataa tatcttgaaa ttattataaa cttgataggt
      661 gacttctttt cctttagttg tttcattcgc ataatgaaca tctacttgtt ctgctcttga
      721 taagtctagc atctcttgaa ctaagctttt catacggctg atttcttgca tgctggcgct
      781 taatgattct tctaatatct caggatcatc tttcccccaa cgatttagca gactaagatg
      841 cccttcaata attgcaacag gtgtccttaa ctcatgagat acgtcttcca cgaattgctc
      901 ttgttgttca atatacaaac gcatacggtc aagcatctca ttgaatattt cagctaaatc
      961 tgccaattca tcatttgtgt cgatttcagg catgtggata tctgattgcg gatctttacg
     1021 tatagtatcc attgtatctc tgagtacctt gagtggtttt aagaaataag aagataaaaa
     1081 gaaacctaaa atactactta agatcaaaga aacaacttct aaaacaataa gagtcaataa
     1141 tagcttattt cgaatatcat agaaagaaga taattcatag aagctttgcg catagccgat
     1201 cttttctctt gtacctttag aatatatagg ctggatagat aaaaagccag tttttccttc
     1261 tagtgtaacg atcgttggaa ctttacgtgt agtctgaatc aggtttcgac tgttttcatg
     1321 ggttttaaaa atcaagttct cgtccaaatc ataaacataa agatccattg atggctgccc
     1381 tagctcagct ataaaggaat cgatcttcat taagtttcct tcgaccagtg aagtatgatc
     1441 tacgacctca tctccgtcat ttgtcgcttt cgtcagatta cgatacgtat ttactaatgt
     1501 cagttcttca tccgagttag ccaaacgaga agctacttct gaaatcgtac gctctacatt
     1561 gtttctttct tttgccacaa tcagattgat agatgattta taggtaatga cagcgaaaat
     1621 tgtaaacaca acgaatatga agaaagaact cgcaaaggcc cacttgattg tcaaggaggg
     1681 ccctttcagt tctctttttt ccgatttttt cggctttatc acgagcgcat cacataacct
     1741 gttccgcgta cagtttggat atagctttcc tccccgggaa cgtcaatttt gttgcgaaga
     1801 taacgaatat atacgtccac tacgtttgtt tcaacttctg tttcgtatcc ccacacttta
     1861 tttagtaaaa cgtcacgtgc taataccacg ttcacgtttt ccattaaagt aagcagcaac
     1921 tcgtattctc tttttgttag ttcaattact tcatttccgc gacgtacgac acggttttct
     1981 ttttcgatag tcaaatcgcg ataagtaata gtagtttgtt tagccacatt tttatcgcct
     2041 tcaatatcga ttcgacgcaa caatgcacga agacgtgcta ataattcttc gatagcaaat
     2101 ggtttgacga tgtagtcatc tgctccgtgg tcaagtccag atacacgatc gatcacagaa
     2161 tctcttgcag tcatcataat gattggtgta tttttgactt ggcgtacacg tctgcagact
     2221 tctaaaccgt tcaattctgg gagcatcaga tccaacaata tggcatccca ctcattattt
     2281 aatgccgcat ctaaaccagt tcggccgttg tagtggactt ctgcgttata cccttcatgc
     2341 ttcaactcta gctcgacaaa tcgtgccaag ttcttttcat cttcaatgat tagaatattg
     2401 ctcatttatt attttatcct ttctattcta ctggcttttt aaaaactcta tcattatacc
     2461 gataatatgc ttaaaaatct atagtcattt tatcatggat agcaatttta aaaaagagct
     2521 gaagtctact cataacaatc gacttcagct ctttcgtcct ctttttattt tctcatttcc
     2581 taacagccga aagactatta ttcttcatgg taccatgagt aatggaagat tccttctttg
     2641 tcgacacgct cataggtatg tgcgccaaag taatcgcgtt gggcttggat tatatttgca
     2701 ggcagacgtt ctgctctgta tgaatcgaag taagcgatgg ctgatgagaa agtcggtaca
     2761 ggtacgccag cttggacagc caaggcaacg acgtctctga ctgattgctg atattttttc
     2821 gtgatttcaa cgaagtaatc atctagcaat aaattttcga tctcaggatt tttgtcatag
     2881 gcatccgtaa ttttttgcaa gaatcgagct cggatgatac atccagcgcg ccagattttt
     2941 gcgatttcac caaatggcaa gtcccaatca aattcttttg aagcgacacg taattgagca
     3001 aatccttggg cataactcat gattttactg aaatacaacg cttctcggat tttttcgacc
     3061 aattcttttt tgtctcccgt gaactcaaaa tcgttcgtac gagacaaaat cttgcttgct
     3121 tgtacgcgtt cttctttata agcagaaata tagcgggcaa atacagattc agtgatcaat
     3181 ggaagtggca cacctaagtc aagcgcgctt tggcttgtcc atttacctgt tcctttattc
     3241 ccagctgcat caaggatcac atctactact ggtttacctg ttccttcatc gtctttacgt
     3301 gtcagaatgt cagcagtgat ttctatcaaa tagctgtcta attcgccttg attccattct
     3361 ttaaagatat cagccatttc ttctactgaa aggtttaaaa tattcttcat taagtcataa
     3421 gattcagcaa tcagttgcat atctccatac tcaataccgt tgtgtaccat tttcacataa
     3481 tgtccggctc catttgggcc aatatatgtg acacatggtg ctccgtcttc tgcttttgca
     3541 gaaatttgct ctaagattgg tgcaactagc gcataggctt ctttttgtcc accaggcatg
     3601 attgaaggtc cttttaaagc tccttcttca ccaccggaaa cgcctgtacc gataaagttg
     3661 atgccagagt tggctaactc ttcattacga cgcatcgtat ctttaaagaa tgtatttcct
     3721 ccgtcaatca aaatatcgcc tttatcaagg tgaggaagta actcttggat tgttgcatct
     3781 gttgccggtc ctgctttgac cattagcaag atccgacgtg gtttctcgat agattctaca
     3841 aattcttcga ttgtatatgt tgctttgaaa tttttatctg ggtgttcttc tactacagcg
     3901 gttgttttag cccctgtacg attgaataaa gccactgaat agccgcggct ttctatgttc
     3961 aaggctagat ttttacccat aacggccata ccgacaacgc cgatttgttg ttttgacata
     4021 tgaactcctc ctacatacgg gatttctccc taatctctta cattatagct taacacggct
     4081 acattgcaaa ctaaagaaca acatatt
//
LOCUS       DACEKH010000141         4037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEKH010000141 DACEKH010000000
VERSION     DACEKH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.148"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            179..775
                     /locus_tag="IW662_002551"
     CDS             179..775
                     /locus_tag="IW662_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0867346.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IW662_002552"
     CDS             899..2698
                     /locus_tag="IW662_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0867347.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IW662_002553"
     CDS             complement(2976..3188)
                     /locus_tag="IW662_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867348.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1186 a          689 c          712 g         1450 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaa
//
LOCUS       DACEKH010000142         3967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEKH010000142 DACEKH010000000
VERSION     DACEKH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.149"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            352..1041
                     /locus_tag="IW662_002554"
     CDS             352..1041
                     /locus_tag="IW662_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0867349.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IW662_002555"
     CDS             1028..2236
                     /locus_tag="IW662_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0867350.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IW662_002556"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IW662_002556"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ0867351.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1304 a          685 c          798 g         1180 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccgttta tcgtaaaaag ggactgtgac aagacttttg tcacagtccc
     3961 ttactat
//
LOCUS       DACEKH010000143         3959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEKH010000143 DACEKH010000000
VERSION     DACEKH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.150"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(607..1164)
                     /locus_tag="IW662_002557"
     CDS             complement(607..1164)
                     /locus_tag="IW662_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867352.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1343..1954)
                     /locus_tag="IW662_002558"
     CDS             complement(1343..1954)
                     /locus_tag="IW662_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867353.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2067..2324)
                     /locus_tag="IW662_002559"
     CDS             complement(2067..2324)
                     /locus_tag="IW662_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867354.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2921..3199)
                     /locus_tag="IW662_002560"
     CDS             complement(2921..3199)
                     /locus_tag="IW662_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867355.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3233..3484)
                     /locus_tag="IW662_002561"
     CDS             complement(3233..3484)
                     /locus_tag="IW662_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867356.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1200 a          810 c          526 g         1423 t
ORIGIN      
        1 tttcttggag ttaaacgctt ctgtcccagc ctccttattc cgaataaatg gtgtccaaaa
       61 gcttgcatct gccagattat ctcatttctt atgacaagct aagcaaatta tctccaccaa
      121 ataatttatt catatttttg acttgcgcat actaactaat tcttaaattt tcatacaaaa
      181 tttctcaatt tcccttaacg agtaatattt atgtacggtg attctcacta gtatcccaat
      241 cactttttaa gttatcccga tttcaattct acttcaaaaa tagttgcttt tatgtatgct
      301 gttagttgat tagatggtca tttgttgagc taggtcgttt attttaaaca agttgtttct
      361 ttagcttata cctatctcgt ctaccaaacg caagatggca aacacaaatg catacgcatc
      421 tgtatttgcc actttgattc gtataaagct aacaactatt aactcctctc agctaatagt
      481 tgctctcttt tttattcaga ttcatttttg ctgatcacag cctagtattt caatggtttt
      541 gaacactaaa ccaaattgtt ccattacaac attcactggc tgatgaaatg gattagtaga
      601 tttcgattat tcaaagcgat tttctattgg tggatcgtgt tcccaactaa taagacggat
      661 tccaaaccta tcgtacagat cttctaaacc acttataaat tttgcaccta tttcaggtgt
      721 tttattgcgt ggatattcta ttgttacgcc attaaatgga taccaattag gattaggctc
      781 tctttttttc tgtacatctc ccatagaata gttagcactt tctgggacat aatatattct
      841 tcgaccatcc tctaattcat ctaataagat atttcgtgat cgtctatcac ttgctaaatg
      901 tagacgccac gtgcgatacc atgccatgct acctgcatct gcagaaaatc catcaaataa
      961 tgcaacaatt gtatcttcta tcacaatcct ttccccatct acttcatatt ctaatctaaa
     1021 atcaaactcc cctcgggtaa tttctggtct tggtggctta ggacccaata ttattctact
     1081 taaaaccgca ttcgttccca gatgaaacac agctataaca acaataatga ttaatatttt
     1141 gtttctattt gataaatttc tcattattcg cacatcacct ctacattctc acatattagt
     1201 tcgtaatact cctttaatat caaaatatct actggtcgta ataatattcg ctatgaattc
     1261 ctactactcg tataagaaag gccaaatcat cagcttttac ttgctgatga tttggcttgt
     1321 ttctatttat cgtaattgtt ttctaatcac caaaattgtt aacaatcggt tcagatattt
     1381 cccagttgat taaggtaatt ccgtattgtg tcaaaacttc ttgcgcttct ccgaaattaa
     1441 gtacagttct tatgtatcca ctttctccta atataaagtc tacctctctt ttaaaacctt
     1501 cctcgagatt tctaagggct atatgtggtt ttaatgacat tgattgtaca ttttccccca
     1561 tataatattg cgcacttcct cgactaaaat agattcctag atcgtcccca aaaggtagat
     1621 cttctctacc actagctaat ctacttctcc atcttcgttc tcttttcata gaacctgcgc
     1681 tgaactcaca cccgttaaac tcagctatga cagtatcttc aattatgtta atctgtccat
     1741 ctagttcgta ttcaatccta aatggaaatt ctccataggt gatttccggt tcgggtattt
     1801 taggtgagat taatgcaaag aaaggaaata ttaagcttgg caacatcaaa aaaactcctc
     1861 ctatgatcac aattcccgat gtaatccaaa atataatttg tacaattctc attgatccat
     1921 tgtattccat tttattcccc ctattactat tcatatccgc ctatctatat cgatcttcca
     1981 atgcagagat aattgcttcc ataccttgat tttttactga gttacctatg cgagatatca
     2041 ttctccacta aggtgcatca tattgtctat ctaacatgat taattctcca ataggtgtat
     2101 actcctccaa aaacaacatt taatgcatct ccctcgacaa cataagctac catgtttgct
     2161 gtatatgtgt ttgagtctaa ttcataagca gataaattta ctgtcgctgg attaaatata
     2221 atcgcattcg tatttatagc tactgaattt gctgcttcag caccaccttt tgaatgtccg
     2281 atcattgtga tttcaatagt aggatttacg tggacagccc tcaccgttta ccctgaccat
     2341 tatagaaatc gcttactttt ttgaactacg caacaaagga tacaatgctc ctaacttcac
     2401 tgtctccatt tttgtccact aaggaatata aaaaataggc attaattcat ttaattcggt
     2461 cacaacacgt taccttttaa atcattatac tgtatctcat acattattat caataaacta
     2521 ttataaaaat gcaaaaaatg ctttatcaat ctattttgta attggtcata atatcattga
     2581 aatcataccc tgtatcgatt tttcctatga atctcgtcta tttgtgattg acgaaaaaga
     2641 ggcaagacag acactggaat ggtaatttta cccactattt tgctccaact atttgtaatc
     2701 gtacccactt ttgaaatacc gagatgatca ttttaattga tatgctgatt cccaagcaaa
     2761 accccaagaa attaaatgct ctatcgcatc tagcttcttg ggttttgctt atataaattc
     2821 caacatttaa attttttatg ctgttcggtc tcagaaatgc cgtttttgct tatatcacaa
     2881 tatcaagatg tcaaattatt cgtgttgccg atgactttga ttaatttttt tcaaattgtt
     2941 tattaagact aatgctgtct ttttgcagat accaatgttc gaacttttta acattatcat
     3001 atgatcctgc aagttttaac ttcttaccat tagatgttat caaagttata attaaccttg
     3061 cctttttccc attaggatct tttattttat gaattttctg caggtaatct ctcctaatgg
     3121 cagctatttt tacctcatca gtgctgataa accattgatc atccacatac atactgcctg
     3181 ttatgttgtg tttttccatt tcttccgaga acgaaaagtt taaggctcgt tcttacttcc
     3241 aaagttgaat gagtccatgg attggactta gtgtgagaat tgagaacaac aataagaatc
     3301 catatcctcc cgcaaacatc cctaaggcta tactgtctct ccatccataa gtaggattag
     3361 aagagaaaag agtcaccgtt tgtaataata tgaaaaggaa taatccagca caaacaccaa
     3421 agataacaac tccaaacatt gaatttttat acaatgacca tttatttata ggcttttctt
     3481 tcattttact tccccctact gttctgttca cacggaatga attcaaagtt cataagccct
     3541 cctctttgaa catatgaata cgacttgcta actatacttt aaaaaagctt gttttaagcg
     3601 gttataggcg agttgtaatc tctattcatc tcattagcaa ataacttgcc gaaaagtcta
     3661 ctgaaattaa agcagatagt ggaagaatta tttagccagg atagacacta tttgtgaaac
     3721 acatgtcttt acttttgaaa ttctatgatg aaaagccttg gaatacagta gctcttaatt
     3781 ggtaatactt tttccattat aaataacctc ttttcatttt atatttatcc cctctaaaaa
     3841 taatatctaa tctaatattt taaacttata tttccctcgc tgtgctaata gagcattact
     3901 aaataaatag aaactagaaa aaagcattac gcctgccaga ataaaccaat tatttgtat
//
LOCUS       DACEKH010000144         3891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEKH010000144 DACEKH010000000
VERSION     DACEKH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.151"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            91..342
                     /locus_tag="IW662_002562"
     CDS             91..342
                     /locus_tag="IW662_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867357.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            324..596
                     /locus_tag="IW662_002563"
     CDS             324..596
                     /locus_tag="IW662_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867358.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            763..1164
                     /gene="tnpA"
                     /locus_tag="IW662_002564"
     CDS             763..1164
                     /gene="tnpA"
                     /locus_tag="IW662_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0867359.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            1176..2321
                     /gene="tnpB"
                     /locus_tag="IW662_002565"
     CDS             1176..2321
                     /gene="tnpB"
                     /locus_tag="IW662_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0867360.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            2559..2822
                     /locus_tag="IW662_002566"
     CDS             2559..2822
                     /locus_tag="IW662_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ0867361.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            2816..3166
                     /locus_tag="IW662_002567"
     CDS             2816..3166
                     /locus_tag="IW662_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0867362.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(3190..3804)
                     /locus_tag="IW662_002568"
     CDS             complement(3190..3804)
                     /locus_tag="IW662_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0867363.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         1407 a          602 c          712 g         1170 t
ORIGIN      
        1 ttcctataca atgagacacg tgttccaaaa aaacattata aggaaagatt gtcggtagct
       61 gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta ttatcgaaca
      121 ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact atgcttggaa
      181 gtaggcgtca aaccaagcac aatcaaacct tctgaatacc aagctttaga actaacgtat
      241 gcgaaattta tcgaaacgaa aaaagctaag actgtttcaa gtgaatggtt agattatttt
      301 gaaaatatca ataagtatgg cgcatgttac atcatgaagt aatcagacgg attcaaaaag
      361 actttagttg tatggaagaa atgaagtact ttgttcaaaa tgttgaagaa cagttatatt
      421 tggaaagcga aacgagtcaa acaaaggtca cagtcagcca taatcgttta gatgtgtata
      481 aacgtcgtcc tgaatcatta aaaaaaggag cggagcttct acgactagaa acagaaaaat
      541 acgtcatgaa tgtttaccta gaaaaaagtg agttggtcga aaattttatg gactagccga
      601 gaacacccgt gattttaatc atgggatgaa tcggattcag ttgtgaccta gctttagcta
      661 ggttaaacaa ctgacaaata taaactaaat aatagtgaaa tgtcttgcaa taattgattt
      721 tgtagaaata catatattaa aataattgcg aggtgttttt gtatgtcaga aattcattat
      781 ggacggggtt atgtctattc tattcaatat catcttgttt ggtgtgtaaa atatcgacat
      841 gatgttttgt atggtcaggt agatattgat gtcaaacagt ttttgaaaca aattgctgat
      901 gataataata taaaaattat agaaatagaa tcagataaag accatattca tttactcatt
      961 gaatgcaccc cacaacacta tattcctagt attgttaaag tatttaaagg cgcatccgct
     1021 agactgctgt ttaaaaaaca tcctgaactt aaacaactcc tttggggtgg acatctttgg
     1081 aatccaagct actttgtagc tactgttagc gaaaatacag aagaacaaat tagaaaatat
     1141 atacaaaatc agaaaaagaa atgaggtgaa tccgtatgct acaacatagg gcttataaat
     1201 ttagaatcta tcctaatcaa gaacaagaaa tattaatgac taaaactatt ggctgttcaa
     1261 gatttgtcta taattatttt ttgaatttgt ggaatcatga atatacgact actggaaaag
     1321 gattatctta taattcttgt tctgccatgc ttccacaaat gaaaagaaat gcagagacaa
     1381 tttggttaaa agaagttgac agtattgcga ttcaatcctc attgaaaaat ctttctgatg
     1441 ctttttctcg cttctttaaa aagcaaaata aacgaccaca atttaaaagt aaaaaacatc
     1501 ctatacaaag ttatacaact aaaaatgtga ataatagtat cgaaattaga aacaatttcg
     1561 taaaactacc taagttagga tttgttaaat ttgcgaaaag tcaagagccc aaagggcgaa
     1621 tcttaaatgc tacgattcga aaaaattcga gtggtaaatt ttttgtttcc atcctgtgtg
     1681 aagaagaaat agttgagttt cctaaatcta attccgctat tggaatcgac cttggtataa
     1741 ctgattttgc aatcctttct gatggtcaaa agattgataa caataaattc acgtcaaaaa
     1801 tggcaaaaaa actcaaacgt gaacaacgta agttgtcaaa acgtgcatta ctagctaaaa
     1861 agaaaggcgt caatctcttt gaagctaaaa attatcaaaa acaaaaacga aaagtagcac
     1921 gactacatga aaaagtaatg aatcaacgca atgacttttt aaacaagttg agcacagaga
     1981 ttatcaaaaa ccacgatatt atctgtatag aagacttaaa tacaaaaggt atgttgcgta
     2041 atcataaact agcaaaaagc atttctgatg tgtcttggtc tagttttgtg actaagttac
     2101 aatacaaagc taactggtac ggtcggaaaa tcatcaaagt agataaatgg tttccatcta
     2161 gtcaaatctg ttcaaattgt ggacacaaag acggcaagaa atctctagaa attcgagaat
     2221 ggacttgtcc tgtttgtcat gctcatcatg accgagatat caatgctagt atcaatattt
     2281 tgaccgaagg tctaagatta catgtcatgg gattagctta gatccaatca tcaaaaaccg
     2341 taggaactac ggggatagct tggtaaataa gagaaaccgc tgtgagtaaa gaaatacgct
     2401 tgcaagtatg ctctattccc aagaatctcg tgactttagt catgagaggt tcaaggattc
     2461 atggaatgtt aatctaattt aatagaaaag gataattaag tatatacgtt ttttcttatg
     2521 tatgttatac tatgtatata cagaaagagg tgtctactat ggaaatgtta cctataaaaa
     2581 aatggggaaa tagtaatggc cttagattgc caaagtatat tatggaatat cttggaattc
     2641 atacagaaga caaagtgaag ataatacaag aagaagtaaa cggtcagaag cggctaatta
     2701 tcgaagcggc taatatagaa aatgaattaa caattgaaca gttatttgaa aattacactg
     2761 atgaaagaaa tcatgtgacg attcaaaatt taggtgaagc agtaggtaat gaaaaatggt
     2821 gagaaaacct agacaaggcg atattctatt attgaataca gcacctcgat ctggacacga
     2881 acagacaggt aaaagacctt atattgtttt aagtcatgat attattgcgg attatagtaa
     2941 cgttgttaca gttgctccaa tttctagtac aactagaaac tatccgctat atgtaaatat
     3001 aaactcagaa tatgaaatga agacaacagg aaaagtatta ttagatcaac taacgacaat
     3061 tgattatgaa gccagagagt gtattttttt ggaaaaagcg catgatagtt tagttgagga
     3121 gttattgatg aaagttagaa cagtttttca aaaagtaagc aaataaaata gaaacagcag
     3181 catgatcaac tattcgtgat tgaccatgct gtcttttttt attctatata cggtttgtct
     3241 cgtaacattg tagtcttttg caatcttagc gatcgcaaca ccttttttta gatcctcaac
     3301 gatgttttta taaacaagac gacgttgggg atcttttgca ttggcactgt atagcttagg
     3361 acgtccttta taaacaccgt tcgcttttgc aatcttaatg ccttgtgcct gacgtcgttt
     3421 agattcggta cgttcttgtt cagcgatcat ggccaaaatt tgaatgatca aatctttaat
     3481 gaatttatcc aggagaggat tgccaattgc ttcggccatg atcggtagac ttgtgatgat
     3541 cagctgcaca tttttcttct tcaagtaatt gactgaagcg atgatctcgt cgtaatttct
     3601 tccaagtcga tcaatagctt ccacaataaa aatatcttga tctcggacga aatccagtgc
     3661 ttcttgaaaa acaggtcgat gagtaatcgt tgcgccagat tgtttctcaa tgaaaattct
     3721 ctctgcaccg aattttttca tttcttcaat ctgccgttgt tcattttgat cgatggatga
     3781 gacacgtatg tatgcaactc tcaaaatttt tacctacttt ctgcaactat ttaaaaaaca
     3841 ccctaatcat acacgagaaa accagatttt tcaatgtcgc ggtcgtgtct a
//
LOCUS       DACEKH010000145         3812 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEKH010000145 DACEKH010000000
VERSION     DACEKH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.152"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            98..2197
                     /locus_tag="IW662_002569"
     CDS             98..2197
                     /locus_tag="IW662_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0867364.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2393..3256
                     /locus_tag="IW662_002570"
     CDS             2393..3256
                     /locus_tag="IW662_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0867365.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3361..>3812
                     /locus_tag="IW662_002571"
     CDS             3361..>3812
                     /locus_tag="IW662_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0867366.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEK"
BASE COUNT         1523 a          636 c          772 g          881 t
ORIGIN      
        1 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
       61 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      121 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      181 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      241 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      301 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      361 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      421 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      481 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      541 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      601 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      661 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      721 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      781 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      841 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      901 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
      961 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1021 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1081 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1141 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1201 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1261 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1321 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1381 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1441 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1501 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1561 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1621 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1681 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1741 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1801 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1861 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1921 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     1981 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2041 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2101 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2161 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2221 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2281 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2341 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2401 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2461 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2521 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2581 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2641 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2701 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2761 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2821 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2881 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     2941 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3001 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3061 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3121 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3181 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3241 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3301 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3361 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3421 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3481 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3541 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3601 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3661 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3721 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3781 catggaatct gtcaggaatg ctatgaaaaa ga
//
LOCUS       DACEKH010000146         3792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEKH010000146 DACEKH010000000
VERSION     DACEKH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.153"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            261..1376
                     /locus_tag="IW662_002572"
     CDS             261..1376
                     /locus_tag="IW662_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0867367.1"
                     /translation="MGGASIVASKIAKGLIDSGHEVFFVSYLESEKPQVESKYYDLYE
                     KKIFLFKNFSRFHKILEYRLFGGFVPSRYIKKEKEQLKKVLKKEKPDVIIFNTFIPSV
                     LFSEFLKYEFPTVKLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCLSQDTKEK
                     LSLYNNHIKVIYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGW
                     AIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEG
                     LPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLM
                     EKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
     gene            1373..2788
                     /locus_tag="IW662_002573"
     CDS             1373..2788
                     /locus_tag="IW662_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ0867368.1"
                     /translation="MRTSIKNFNLWKEWYLYIFFIGMIIRAINFYSLLPSTFDSIFFK
                     LIGISGVLLLGIDFIIKFNKRKVTYNILLIAYMVILVITSFVHKEYGLGENLKTIMWA
                     ILQYFLIYQFAFENSDNKRFFKRISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHR
                     IRIGFLESRLFGTFSDINNAAIASLVVIVLILFYLYNKKVTGLNKIIFLINLPLQFIY
                     IVLSGSRSTYVVSIIIVAISSFIVVLKRNGTKKDLKNYILAIVSSIGSVLILFALFNA
                     TKILFQELLPHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAENTDISNA
                     RITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGI
                     LGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNELVYENTV
                     GTFVFWLFLGRVNSKNNSSYF"
     gene            2821..3768
                     /locus_tag="IW662_002574"
     CDS             2821..3768
                     /locus_tag="IW662_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0867369.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
BASE COUNT         1342 a          464 c          660 g         1326 t
ORIGIN      
        1 tttatagaat taataatttc ggcaatagtc gtttttctat taaatgaata tttagcaaat
       61 ggaggcagta cgttccttca atttataact aaaaccttaa taactatagt aacaacatcg
      121 atattatatt tgttattata ttttagaaga gaagaattta catttatcaa aaaaattctt
      181 tttaggtata ttttaaaaaa atagctttct ataaaggagt gttttatttg aaaatagcta
      241 tactatctcc agctttctca atgggaggag ctagtatcgt ggcctcaaaa attgcaaaag
      301 gattaataga tagtggccat gaagtttttt ttgtttctta cttagaaagt gaaaaaccac
      361 aagtagaatc taagtattat gatttatacg aaaaaaaaat ttttttattt aaaaattttt
      421 ctcgatttca taagatatta gagtatagat tgtttggagg ctttgttcct agtagatata
      481 taaaaaaaga aaaagaacaa ctaaaaaaag ttctaaagaa agaaaaacct gatgtcatca
      541 tattcaatac ttttatacct agtgtactat tctccgaatt cctaaagtat gaatttccaa
      601 cagtgaaatt aattacttgg atgcactctg atcctgatta cagcttaaat catattgcta
      661 aattttataa aaaagaatat agaaaaagtt ttgaacgcgt ggattccatt gtttgtcttt
      721 cgcaagatac aaaagaaaaa ctttcattat acaataacca tattaaagtt atttataacc
      781 cattgacatt aagcggctct agtttaataa gtaaccttga taaaaaaatt atttcattta
      841 ctgccagata cgatataaat attaaagggt ttgattattt atgtgaagta gctaaaaact
      901 tacctgttgg atgggctata aggattgctg gagatggtac agaagaagaa cgtaaagagc
      961 tgaaaagatt aattgagaaa aatggtgtgt tagataaaat tatttgcgca ggtgcattat
     1021 ttggagatga attaatcaat cattatatag aaagttctat tttcatatct acttctagaa
     1081 gtgagggact accattagtg ttgatagaag caatgactta tggattacct gtaataagtt
     1141 ttgatcatgg tggtgctaag gaaatattac aagatggaaa ttatggaatt ttaattaaga
     1201 attttgacgt acaagatatg aataatgaga tagaaaaatt gtctaataat cgtttactta
     1261 tggaaaaata tcaaaaattc tctttagaaa gagctaagga ttttaatgaa aaaaaaataa
     1321 ttggtgaatg gcaaacattg ttaaatgaat taggaaatga ggatgttaga aattgaggac
     1381 ttcgattaaa aattttaatt tatggaaaga atggtatcta tatatatttt ttatagggat
     1441 gattattcgt gcaataaatt tttatagttt attaccgtcc acttttgata gtattttttt
     1501 taaacttata ggaatttcag gagttttgct attagggata gatttcataa ttaaatttaa
     1561 taaaagaaaa gtaacttata acattttatt gatagcatat atggtgattt tagtaataac
     1621 gagttttgtt cataaagaat atggtctagg agaaaactta aagactataa tgtgggcaat
     1681 cttacagtat tttttgattt atcagtttgc ttttgagaat agcgataata aacgattttt
     1741 taaaaggata agttggggat ttatttatag ttggtttgtt ttagtatcaa tgtcaatggt
     1801 tcttttcttt ggtaaattcg gctatgagaa atattataat gctagacata ggatcagaat
     1861 tggtttttta gaatcaagac tgtttgggac gttttctgat attaacaatg ctgctatagc
     1921 atctttagtt gttattgtcc taatcttatt ttatctttat aataaaaaag tgacaggatt
     1981 aaataaaatc atttttttaa ttaatcttcc attacaattt atttatatag tattaagtgg
     2041 gtcacgatct acctatgtgg taagtataat aatagttgcc atatcatctt ttattgttgt
     2101 gttaaaacga aatggaacaa aaaaagattt aaaaaactat atccttgcta tagtatcttc
     2161 tattggttct gttctgattt tattcgcttt gtttaatgca actaagatac tttttcaaga
     2221 attattacct catttaaaag aaataaattt ttttaatttt acagagcgtc ataaagaatt
     2281 aatgaataat agtcaacata ttaataatac tcaaacacct gataatactc ttgttaggaa
     2341 agacgtagct gaaaatacag atatttctaa tgctagaatt actatatgga aaagtgcatg
     2401 ggaaatattt aaaacaagtt ggcttatcgg agtctctccg aaaaatgtag tatcttatgc
     2461 acagtcaatt attccagatg gatatattgc gaaagtagga attgccattc ataatgcata
     2521 cttgaatgta ttaacatcga caggtattct aggaataatt cctttcatgg cttttttaat
     2581 aaagagtttt gtgctgaatg tttatcgaat catctataat aaatgcattg gtaaagatta
     2641 tcattttgtt tactgtttag ttattttatc ttatgcacta tacgcagctc ttaacaatga
     2701 attagtctat gaaaacactg taggaacgtt tgttttttgg ctatttttag gaagagttaa
     2761 tagcaaaaat aattctagct atttttagta ataaatttta tcttgaaaag aggagaaaaa
     2821 atgttgaatt acttgattac cggtggcgcc ggcttcattg gctcgacgct cgcgaattat
     2881 ctaggaaaag aaaacaatgt agttgtgatc gatgatttat cgatggggaa aaaagagaat
     2941 ctggatgaat cgaagcacat cacgtttatt gaaggggatg tcgctgacaa aaatttgatg
     3001 gaaaaggtca tgaaagaata ccatttcgat tatattttcc atctagctgc tgtagctagt
     3061 gtggcagatt ctgtagaacg tcctctagaa actcatcgtg taaattttga cagtgcgctt
     3121 ttattgcttg aattggtacg aaaatatcaa tctggtttaa aacgattggt atttagttca
     3181 tcagctgcag tttatggcga tgaaccaacg ttgccaaaaa aagaagaatc ggtcatccgt
     3241 cctttaacac catatgccat cgataagttt gcagcagaac aatatgtttt ggattactgt
     3301 catttatatg atgttccgac tagtgctgtc cgatttttta acgtttatgg tccaaatcaa
     3361 aatcctaact ctccatatag cggagttatt tcgattctag tggaccgata caaaaaacaa
     3421 ttggcaggag aaaaaacaga atttacttta tttggtgatg gtagccaatc tagagacttc
     3481 gtttatatag aagatgttat acaagcactt ttacttgttg ctaaggaaga aaaagcttta
     3541 ggacagcaat tcaatgtcgg tacaggaaaa agcactacgc tacttgaatt gattcattca
     3601 attgatcaaa tcttaggaac agagctagcg cttaaatatg aagcggagcg ttcaggggat
     3661 attcgtgatt ctttagcgga tatttcaaag atccgttctt taggttatca acccaaattt
     3721 gatatattga acggaatgga acgttattta aagacagaaa tcgattgaaa caaaaaacca
     3781 gcatcctgct gg
//
LOCUS       DACEKH010000147         3735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEKH010000147 DACEKH010000000
VERSION     DACEKH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.154"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            119..2560
                     /locus_tag="IW662_002575"
     CDS             119..2560
                     /locus_tag="IW662_002575"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ0867370.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2890..3594)
                     /locus_tag="IW662_002576"
     CDS             complement(2890..3594)
                     /locus_tag="IW662_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867371.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1205 a          654 c          815 g         1061 t
ORIGIN      
        1 atataaaaaa gagaatattt aaggattgtt aaaatttaat cttagaaaaa aataactgga
       61 acaagagcta tttgttttta cagcaaagaa tggtatattt ttgtaggagg tggcttactt
      121 gaaagaagtc gttatgatag atgctgcaag aacgccgata ggaaagtatc gcggaagttt
      181 aagtccattt actgcagttg aattaggcac acttgtgact aaaggattat tagataaaac
      241 aaaactgaaa aaagataaaa tcgatcaagt aatatttgga aatgtcctgc aagctggtaa
      301 tggtcaaaat gttgccagac agattgcttt gaacagcggt ctccctgtag atgtcccagc
      361 aatgacgatc aatgaagttt gcggatctgg tatgaaagca gtgatcttag cacgccagct
      421 gatccaatta ggtgaagcag aacttgtgat agccggcgga acagagagta tgtctcaagc
      481 acctatgctg aaacagtatc agtcagaaac aaatgaatat ggtgaaccaa tttccagtat
      541 ggtcaacgac ggattgactg acgcattttc aaatgcacat atgggattaa ccgcagagaa
      601 tgttgcaaca caattttctg tgagcagaga agagcaggat cgctatgcct tgtcgtccca
      661 gttgaaagca gcacatgctg tcgaagccgg tgtattttct gaggagatca tcccagtcaa
      721 gatttctgat gaagacgtgt tatctgagga tgaagcagtt cgtggaaata gtacattgga
      781 aaaactgggc acgttacgta cagtattctc agaagaagga actgtaacag caggaaatgc
      841 ttccccgttg aatgacggtg cctctgtggt gatccttgca tccaaagaat acgcagaaaa
      901 taataatctg ccttatttag caaccatcaa agaagtagcg gaggtcggta ttgacccaag
      961 tatcatggga atcgctccaa tcaaagccat tcaaaaattg acagatagat caggaatgaa
     1021 tttatcaaca atcgatcttt ttgagatcaa tgaagcattt gcagcttctt ctatcgtagt
     1081 tagccaagaa ctgcagttag atgaagaaaa ggtcaatata tatggaggag caatcgcatt
     1141 aggccatcca attggtgcaa gcggtgcacg tatcttgact acattagctt acggattgct
     1201 tcgtgaacag aagagatacg gtattgcttc attatgtatt ggtggaggtt taggcttggc
     1261 agtcttgtta gaagcgaata tggagcaaac acataaggat gtgcaaaaaa aaaagtttta
     1321 tcagcttacg ccatctgaac ggcgcagtca attaatagaa aaaaatgtat tgacacaaga
     1381 aactgcgttg atttttcagg aacaaacact ttctgaagag ctctccgatc acatgatcga
     1441 aaaccaagtg agtgaagtgg aaatcccaat ggggatcgcg cagaattttc aaatcaatgg
     1501 caagaaaaaa tggattccga tggcaacaga ggaaccatct gtcattgcag ctgcaagtaa
     1561 tggtgcaaaa atctgtggaa atatctgcgc agaaacacct cagcggctga tgcgagggca
     1621 aatcgttcta tcaggaaaat cggagtatca agcagtgatc aatgcagtta atcatcgaaa
     1681 agaagagctg attctttgtg caaatgaaag ttatccttca atcgtgaaac gcgggggcgg
     1741 tgttcaagat atctctactc gggaattcat ggggagtttc catgcatact tatctattga
     1801 ttttttagtg gatgtaaaag atgcaatggg cgctaacatg atcaactcga ttcttgagag
     1861 tgtggcaaac aagttacggg agtggttccc tgaagaagag attctattca gtattttaag
     1921 taattttgct acagaatcat tggcttctgc ctgttgtgaa atcccattcg aacgcttagg
     1981 gagaaacaaa gaaatcggtg aacaaattgc caaaaaaatc caacaggcag gggaatatgc
     2041 gaagcttgac ccttaccgtg cagcaacgca caacaaaggg atcatgaatg gtatagaagc
     2101 tgtagtggca gctacaggaa atgatacacg tgcggttagc gcttcgatcc acgcctacgc
     2161 ggcaagaaat ggattatacc aaggattaac agactggcag atcaaagggg ataaactagt
     2221 tggaaaacta acggtgcctt tggctgttgc cacagtaggc ggagcttcca atatcctgcc
     2281 aaaagcaaaa gcatctttag ctatgttaga tattgattca gccaaggaac tagcacaagt
     2341 aattgctgca gtagggttag ctcaaaacct ggctgctttg cgtgcattag ttacagaagg
     2401 aatccaaaaa ggccacatgg gacttcaagc tcgttcatta gcgatctcaa ttggggctat
     2461 tggtgaggaa atcgagcaag ttgcgaaaaa attacgtgaa gcagaaaaga tgaaccagca
     2521 gacagctata caaatattgg aaaaaatccg ggaaaaataa aaaagcaata tctttagaag
     2581 aataaagcaa gatttacagt tattttattt actgcagatc ttgctttttt ctctatcaga
     2641 aaatatgttt attattagaa ttttttatag ttaaggttag gcatcttaag aaatcaggca
     2701 actccaagat atcttcaggt agaagagaag tcgcctaaca ttgtaggatc ataggtgatt
     2761 tgttggacag gtcaagctga tatttgagcc tgatgttaga aggaatttct ttttacttgc
     2821 tgtagaaatt attatatgac taaaaaggct gggattttaa atcccagcct tttatgacaa
     2881 atagaatttt tatcgatcag taatcgctgt cgctgcaaat tgttcgatca aaggaatcaa
     2941 agtcaatttg actttgtatt tccgttcgag tgctttttcg ataacgaact cattttcagt
     3001 agtcatagaa atagttgcta atttacgagg ttcatcagca tttttttcat taataactac
     3061 ttcatgttca ttttgttctg tgtaaaattt aggatactca gcaacggctt ttgaaaaatc
     3121 agcaaaactt aaagcggcat tcgtatcagt tgagcgtgta ttttcagttg tgtccgcttt
     3181 tggtttttcc gttttttctt ctggcacact gactgtttct tctgtaactg gtgtcgcaac
     3241 agcttgcgca gttggttcat cgtttttcaa tcgttgatca ttccagtaag acacgatttc
     3301 aataagaaga ttgtcacgga atcgttcgaa aatcccgttt tctcctgaac gaattctctt
     3361 taatacagta ttgaagttgt catcgattgg cacgttgatc cgtgtatctt catttgtttt
     3421 cccagctaat ccgatcgtta agatttgacg gtttttggga tcaagcatga cttgtaacgg
     3481 caaaccataa cgtcgcaagc tctcttcttc tagagaacgg aataattgtc ctcttacacc
     3541 atccaatgaa ggacggaatt gatttaataa tttttgtttc tgttttttct tcatatgttc
     3601 tctccttcgc tcataccctt tcgattataa gaaaaaaaca gccaaatgaa aaggagtaat
     3661 gcaaaaaaac ttgcgtatac gcaaattaca acagaaaaat ttgcgtattt tcccgttttt
     3721 tgaaagaaaa ataaa
//
LOCUS       DACEKH010000148         3706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEKH010000148 DACEKH010000000
VERSION     DACEKH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.155"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            72..1019
                     /locus_tag="IW662_002577"
     CDS             72..1019
                     /locus_tag="IW662_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAQ0867372.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            complement(1316..1864)
                     /locus_tag="IW662_002578"
     CDS             complement(1316..1864)
                     /locus_tag="IW662_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867373.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1865..2722)
                     /locus_tag="IW662_002579"
     CDS             complement(1865..2722)
                     /locus_tag="IW662_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0867374.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(3008..3295)
                     /locus_tag="IW662_002580"
     CDS             complement(3008..3295)
                     /locus_tag="IW662_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0867375.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3285..3614)
                     /locus_tag="IW662_002581"
     CDS             complement(3285..3614)
                     /locus_tag="IW662_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0867376.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT         1127 a          677 c          528 g         1374 t
ORIGIN      
        1 aaaaagacta aattcaattt ttttggtata atgagaaaaa atatctattt gaaaaagtta
       61 ggagctatac tatgcatctg gaattctctg actatgaatc actccttgta aaaatttgtg
      121 aagactatta tctcgaggat atcaatatta gtgaaatttc taaaaaatat gggatcacca
      181 gatataaagc tttaaaatat attgaagatg cacgtgaaaa acaattagtt agtatttcaa
      241 ttaattctcc gtatgctagg aattatgagc tagaagaaat cttcaaaaat tactttaaaa
      301 cgaatatcta tatattaaaa agtagtgaag acttatcaca acacgattta ttattttcaa
      361 aatttgctgc cgattacatt caagaattaa ttaagaaagc caatgtggtt gcactatctt
      421 ggggagatac cgtttacaaa gtgattgatc agtttaaaat gggtgctcgt gaagaattaa
      481 tttttactca attcattggc gaaatcggaa aatataattc cttagctggc tccacacgat
      541 tagttcaaaa agctgctgaa cgttatgagt cttcttattt gactctcagt attcctttat
      601 atgtcatcaa tgaaactgca cgtgaactaa tggctctaga accttcttta gctaatacct
      661 tagcaaccgc ttcgcatgca gatatgttaa tttctggaat tggcacctta tcctctattg
      721 attctgtcga accctggaac aagcatcgta aaatcctatt tccaagcatg acggatactg
      781 ttggatttct ttacggaaga cccttcaata tcaatggtga attcatcaaa gcatctgagg
      841 ataagacttt tggtctttct ttaaaagaaa ttttcgatat tccaaaacga attggtattt
      901 gtaaaagtaa gttcaaagcg gagagttgtt taggcgcctt gaatggtaat ttttttacag
      961 atttattttt ggatgaaaaa actgcttgga aaatactttc tttgataaaa aaagagtgag
     1021 tcatgtaaaa tcctccaaaa aatttagatt tttggtacta cttttttgga gggcagtaca
     1081 atgattcact ctttttattg gatatcaaaa tccctttttt tgagaagctt gtcttgatat
     1141 tttttgaact tttccaagcc atactccatt acttttcaag atttaatttc tttgtattca
     1201 ctgaataatt tccacgtttt ttcgttaatg ttacttctta ttaattttat tgtacttctc
     1261 atgcaatagg atcatatatc cttctaaatc ttttttatgc tataaaaagg gggttctatc
     1321 taactttatt attcattccc caccctttgc gcagtgagtc ttcggtatct tgttttatct
     1381 gactggtagt tcttattatt tctccacggt catctaattc tggaacaatt ggatattttt
     1441 ctctgaacaa gtctagttct ttcaccacgt cattaaacaa atcttgctta tcttcttgcg
     1501 taagattctg tttaatatac tcatctaaag cttttctaag caattcatct cttgtcatat
     1561 tagtctcata cttttctact aaaactctgg aagtcgcatt taacaaagtc gacagtttta
     1621 aaggatgctt ggtggaataa accttatcaa actttctgat tggtgcgcca actttttttg
     1681 ttcttcctct actctgtctt tcaacattgg aggaagtttt tatttcttga acttggtgca
     1741 cttctaattg cttattttca gatggtgtaa ttgcttgctg ctttttcttt gcttctgctt
     1801 ctttttttat tttggcgagt cctttgttgt tcccaaaaat atcaagtcct ttgatgtcag
     1861 ccaattactc cacctccgca taccaattct cgtgttcttc caattctttc aaaatactga
     1921 taaataattc gtggcttttg cgatcccatt gttttaagag acctttcttt gtcttctcca
     1981 tcgtaatacc tgatccgtcg tatcgtttta agcgcgcatt tttgagtaca acatttttaa
     2041 tcaaatgttc cccataaatc tcttgggctt gtttatacat ttcctgatct actgcgtcac
     2101 cgctttccaa catgaaagga ataacaccag caacatataa atttgtatca aattctttca
     2161 caaaaaagtc catatactca agatattgca tcgcatttct catcgatttt acttgggtct
     2221 gtaagacgac gattgcccag tccgcaatgt acatcgcgga tttactatat acagaaatag
     2281 aaggtgggac atctaaatag atgacgtcaa actcatcaaa taatggttct agcttttctt
     2341 tcatatattt gaattgggat aatgaatcat tatattggtc tttgatccaa tcatcataca
     2401 tttcaaagga catatctgct ggaagtatat agaggttatt gattacttta tagacagcct
     2461 ctttaggtgg aagattgttt atcaacacgt cagtcacagt attttcgtaa acaatcggtt
     2521 caccttctcc gcctccttct tcgttagtga agttcatatg cgttaagttc atcacatcag
     2581 aagtatttcc ttgcatatcc aagtcaatta tcaaggttct ttttccttca ttaactgctg
     2641 attcaaaccc taacatttgc gcgcacgtac ttttacctac gccaccttta aaattaccaa
     2701 ttacatattt cttcgctacc acgttcctac ctcttttctt ttagcaatat tcatttgata
     2761 ctattataac atatttattt ttagttacta ccaaaaaaaa gatatatatg tactacctta
     2821 taattaataa gtaaaaggta gtacataaat aaggaaaagt tgaaatatat tgatttatag
     2881 ctttcctcaa agtaatgacg ttatttaatt tatcgagaag cagagaatac tcgcgacttt
     2941 agtcatgtag acgaatcaga ttttcgataa aaaagaagcc gtccttctta agaacgacaa
     3001 caaatcacta gattaatttt ttcaactgtt cataaaagtt ttcatgcgtt ccacataaca
     3061 atactggaat aaccttgttt tctataatct cgtaagctac tctatatgtt gtgcccgcat
     3121 atttgaaccc actagaccaa atacctgata aatccccagt tttttgttca cccttatatg
     3181 gatcaatcgc aatttcatca taaattaaat tcaaaaattt ttctttcaat ggcttctctt
     3241 ttagcttttt taaatactta atgacatgtt ttgaaggaac gatttcataa gccaatttct
     3301 gttgccaact cctctttagt cattacttta gaattagtaa gtgtatcttc agcaatatct
     3361 ctaaaggaag aatgcatttt gttttttcta ttcttaaatt ctacaagaat ctcatcttta
     3421 tcgtagcctt ctgcaattat atcagacaaa atatcttcgc taaaatcata aaattctttt
     3481 tgtggttcaa caaattcata aagaatacca ttctctacca actttgcttc aacttcgact
     3541 ccattaggaa cattaaaatc tttaggtact gtcaacatga ttgaattgcc ttgttttctt
     3601 gtttttgtaa tcatttaaat ccctcatttc taacagtatg taattactta gtaaatacat
     3661 tataccacga gataatgtat ttacaagtaa ttactgtgac tcgtaa
//
LOCUS       DACEKH010000149         3665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEKH010000149 DACEKH010000000
VERSION     DACEKH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.156"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..340)
                     /locus_tag="IW662_002582"
     CDS             complement(<1..340)
                     /locus_tag="IW662_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0867377.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
     gene            617..1571
                     /locus_tag="IW662_002583"
                     /pseudo
     CDS             617..1571
                     /locus_tag="IW662_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 105 protein"
     gene            1588..2612
                     /locus_tag="IW662_002584"
                     /pseudo
     CDS             1588..2612
                     /locus_tag="IW662_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2618..3097
                     /locus_tag="IW662_002585"
     CDS             2618..3097
                     /locus_tag="IW662_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ0867378.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            3113..>3665
                     /locus_tag="IW662_002586"
     CDS             3113..>3665
                     /locus_tag="IW662_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ0867379.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
BASE COUNT         1123 a          621 c          836 g         1085 t
ORIGIN      
        1 ggcgaacata attctttcca aaagttgaga aatgggggat tttctcctcg aaggagtagc
       61 ctaaaaacca gcggtaagcg acattggtat cgacttcctt aatggtttgt cgcatggaac
      121 ggataccgaa taaatactga ataaacacca atttgatcag aatcactgga tcgatacttg
      181 ggcgacctaa atcggagtaa gtggcttcga cgattggata aataaattca aaatcaagcg
      241 cggcatcaat ctttcgaacg agatgatctt gaggaactag atcatcaatc gtaagcatcg
      301 caaattgatt tctaccattc gtattgtttt tagacatcat agctatcact ccttgcattc
      361 gttttcataa atctattata aaagaaaaga ctgtagacat catccataaa atggactttg
      421 tctacagtct ggaacttgga aaacgttgtt tctccaagtt ctttttttaa tttccaagta
      481 actgaaagtt acttttgtta tttaacattt aggtcaattt tagacagttt ttgttcaaaa
      541 agcgatattt atagtggacg gtattactga tatattaagg atataaaatg aaagcgttta
      601 aaaaggtggc gtaaagatga ttattaaaga agagattttt gtacctaaag aattagtgaa
      661 agaaaaaatt gacctgctcg ttgaaaacct gacaacgatc aaagacgata atggtgaatt
      721 tttgttgggc tttgatggac tgaaagtaga tgacaagagc tggacagtgt ggaattggcc
      781 acaaggtgtc ggtctatatg gtatttataa aaattaccag atgacaaaaa atcctcgcgc
      841 ttatcaagtg gtcaatgaat ggtttgagga tcgtatggaa gaaggcgctc cgcccaaaaa
      901 cgtcaataca atggcaccgc tattgactat ggcttacttg tatgaagaca cgaaagattc
      961 acgctatttg ccttatttag aacaatgggc agaatgggtc atgaatgaca tgcctcggac
     1021 aaatgaagac gggttacagc atgccacata tggaccggaa aataagaatc agttgtggga
     1081 cgatacattg atgatgacag tgcttccttt ggcaaaaatc gggaaactgc tggttgacga
     1141 ttgctattcc agagatcatt gaaatcttag aattatcgaa agaagatgca ttgcgcaaat
     1201 tattgatcga aacacttgaa gcacaagtgc gtgcattaaa aacttatcag tctgaaagcg
     1261 gtttatggca tacgttgttg gatgatcctt cttcttacgt agaatcttct gcaactgcag
     1321 gatttgctta cggtattttg aaagcggtcc ataaacgtta tttaccacaa gaatataaag
     1381 aagtcgctta tcgtgctatc caaggattgc ttgaacaaat cgatgaaaaa ggagaagttc
     1441 aaaacgtttc aatcgggaca ggaatggggg acagtttaga tttttatcgg aatatcggaa
     1501 tcacagcaat gccttatgga cagtcgttaa cggtgctttg tttgacagaa ttgctcgtct
     1561 cttattgtta aaagaaagga aacccatatg aaaaaaacat cactgctgtt tgcagcaagt
     1621 tcgcttagcc taggtctgct aggtctaagc ggctgcggaa ataattctag tgtggcaact
     1681 gtcgcagatg cagacgtaaa tgagatagtt accttgcgct ttgcttatgc aagcaacagc
     1741 cagtaattga tgcaatgaat gaatttgggc gtttagtaaa agaaaaaaca gatggttccg
     1801 tacagatcca gtatttccca gatggacaac tgggtggaga acgtgaactg atcgaattga
     1861 cacagtcagg agcagtagat ttcacaaaag taagtgcctc agcgttggaa agtttctcta
     1921 aagattattc gattttttcc gttccttatc tattcaatga tgaagaacat ttctttaagg
     1981 tgatggataa tgaagaaata atggagccaa tctaccaatc gaccaaaaat ttaggattta
     2041 ccggattgac ctattatgat tctggtcagc tcagctttta tatggtagac ggtccgatcc
     2101 atacgccgga agatttgaag ggaaagaaga tccgtgtgat gcaaagcgaa accgctatca
     2161 aaatggtaga gcttttaggc ggttcgcccg tacctatggg aagtgatgaa gtgtacacgt
     2221 ctcttcaatc caatttgatc aacggatcag aaaacaatga atttgtcttg tatacagctg
     2281 gacatggcgg ggtagctaaa tactattcct atgatgagca tacacgtgtg ccagatatca
     2341 tcatcatgaa tgatgccatc aaagaacagc taactgatga acaggaaaaa gccattgaag
     2401 aagcagcgaa agaatcaacg gtttttgaaa ttgaagcttt tgcaaaagca attgaagaag
     2461 aaaaagcttt agcacaagaa gaacatggag tccaattcaa tgatgtagat aatacaccgt
     2521 ttcgagaggc agttgcccca cttcatgata tattcaaaaa cgatcctacc tacaaagacc
     2581 tttatcaaaa gatccaagag caaggagcgt agctgtaatg aaaatgattt taaacaaggt
     2641 attagagatc ctgggagctg ttttgcttgt tgggatggtc gctgttgttt tatggcaagt
     2701 attttccaga acggttttag gaaatccaaa tacggtcacg gaagagcttg ttcgattcgg
     2761 tcttgtctgg tttgccatgt tgtcttccgc gtatgttgtg ggacaaaaag cacgtttagc
     2821 cgtaacggtt ttaagtgaaa aattaactgg caaaaaaagt aaaattttag aagttgttgt
     2881 tcaaagttta tttctggtat ttgctgcgat gatcatggtg tatggcggat ggaatgctgt
     2941 cacattgaca atggggcaga tctcgccttc tttaagtatt cctatgggat atgtttatct
     3001 gtcagtacct gtatcggggg tactcatcat tatctatagc ctgatcaatc taatcgatac
     3061 attaagcaac aagccagcaa ttcaagaaaa aatataaaaa tggaggaata aaatggcaat
     3121 agaagccgga ttagtgttat tttttgtatt tgttgtgttg cttgtattag ggatgccgat
     3181 tgctataagt attgcattct catcgatgat gactttattg ttagttatac cgtttgatgt
     3241 ttctgctttt agttcagctc aaaaaatggt gggaagtatc aatagttttt cgctgatcgc
     3301 tattccgttt ttcgttctct cgggcattat catgaataat ggagggatag ctaagaaact
     3361 agtggatttt gcgatgttgt tcgtcgggcg cgtgccgggg gctttggctc atacgaatgt
     3421 gttagggaat gctttatttg gttccatctc tagctcggcc attgccgctt ctaccgcgat
     3481 tggtggagtg ttgatccctc agcaagtgga agaaggatat gaccggaagt ttgcgacagc
     3541 ggtcaatatc gcgtcagctc cgacaggtat ggtcattcct ccaagtactg cttttatcat
     3601 gtattcactt gttgctggtg gtgcttcgat ctctgctttg tttatgggag gttatctcgt
     3661 aggcg
//
LOCUS       DACEKH010000150         3637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEKH010000150 DACEKH010000000
VERSION     DACEKH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.157"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            155..1384
                     /gene="arcA"
                     /locus_tag="IW662_002587"
     CDS             155..1384
                     /gene="arcA"
                     /locus_tag="IW662_002587"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ0867380.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            1475..2494
                     /gene="argF"
                     /locus_tag="IW662_002588"
     CDS             1475..2494
                     /gene="argF"
                     /locus_tag="IW662_002588"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ0867381.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            2609..3556
                     /gene="arcC"
                     /locus_tag="IW662_002589"
     CDS             2609..3556
                     /gene="arcC"
                     /locus_tag="IW662_002589"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ0867382.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
BASE COUNT         1257 a          642 c          770 g          968 t
ORIGIN      
        1 ataaattacg cttaaaaata gaaaaacgtt taattattta attatcgaat attaatgtga
       61 ggaatgtctc attttgaatg agacaaaatt atgaaagtga ttacaaaagg aggatatcct
      121 atacatgtaa tcaaaaagaa ggaggcaaca caacatggat aaacctattc acgttttctc
      181 tgaaataggg aaattaaaga cagttttact gaaacggcct ggacaagaag tggaaaattt
      241 gacaccagat attatggatc gtctgttgtt cgatgatatt ccatatcttc cgattgctca
      301 agaagaacat gacaactttg cgaagacatt gcaaaatgaa ggcgtcgaga ctctttactt
      361 ggaaaaatta gcggcagaag cgattgacgc aggcaatgtc aaagaacaat tcttgaataa
      421 aatgttggat gaatctcata ttgcatcgaa tgctgtaaga gagggtcttc atgagttcct
      481 tctatcaatg gaaacgcaag aaatggtaga taaaatcatg gctggggttc gtacaaaaga
      541 tatcgaagta cgttctagca gtctatatga tctgtcagca gacgatgact atccatttta
      601 catggaccca atgccaaact tgtactttac acgcgaccct tccgcatcaa tgggaaatgg
      661 gatgacagtc aataagatga cttttgaagc acgtcgtcgt gaatcgatgt ttacagaata
      721 tattttgaaa catcatccaa gatttgcaaa taaaggaatc gaagtatggt tggatcgtga
      781 aaatccagat catatcgaag gcggagacga attgatctta agcgataaag tagtagctgt
      841 tgggatttct caacgaacta atgcgaaagc tttagaaaaa ttagcacgcc acctattcgc
      901 aaaaaattca ggatttgaaa aagtgttggc aatcaaaatt ccgaacaacc gtgcaatgat
      961 gcatttggat accgtattta caatggtaga tcacgataaa ttcactatcc atccagcaat
     1021 tcaaagtaaa gacggtaaga tggatgtctt tacaatcgaa ccagatggtg atgatatcaa
     1081 gattactcat tcagataact tacacgaaac gatgaaagct gctcttggat tagatgatct
     1141 tgtactgatc ccaactggta atggagatga gatcgttgca cctcgagaac aatggaatga
     1201 tggatcaaac acattggcaa tcgctccagg tgttgtagtc acttataacc gaaattatgt
     1261 atcgaacgaa ttgcttcgta gctatggcat caaagtgtta gaaatcaatt caagtgaatt
     1321 gtcacgaggt cgtggtggtc cacgctgcat gagccaacca cttgtccgtg aagacttgaa
     1381 gtaatttgtt tcctgacggc agtcttactg tcagctgccg tcgggatttc ctctttaatc
     1441 ttaaataaaa acaatctaaa ggagaatgtt taatatgaaa gaatcagtat tccaaggaag
     1501 aagcttatta gcagaaaaag actttacaaa agaagaatta caatatttga tcgatttttc
     1561 agaacacttg aaagacttga aaaaacgtgg tatcccacat cactatctag aaggaaaaaa
     1621 tattgcgctt ttatttgaaa aaacatctac acgtactcgt tctgcattta ctactgcagc
     1681 gatcgatcta ggtgcacatc ctgaatatct aggtgcaaat gatatccaat taggtaaaaa
     1741 agaatccaca gaagatacag ctaaagtact aggacgtatg tttgacggaa tcgaattccg
     1801 tggattcagt caaaaaatgg tagaggaact agcagaattc tctggtgttc cagtttggaa
     1861 tggcttgact gatgaatggc atccaacaca aatgatcgct gactttttaa ctatccaaga
     1921 aaactttgga acagtagaag gtatcactgt tgcttactgc ggagatggtc gaaacaatat
     1981 ggctaactct ttattagtca caggtgcaat tttaggagcg aatatgcgta tcgttgcacc
     2041 aaaagaatta caacctgaag aagaaatcgt caaaatggca gaaggctttg ctgaaaaatc
     2101 cggtgcacaa ttgatgatta ccgacgatgt tgataaaggt gtagacggag cagacgtcct
     2161 ttattctgat gtatgggtat caatgggtga agaagataaa ttcgaagaac gtatcaaatt
     2221 gttgaaacct taccaaatca atatggaaat ggtagaaaaa acacacaaca cagatcgttt
     2281 gatcttcttg cactgcttgc cagctttcca tgatacaaac actgtttacg gtgaacaaat
     2341 gaaagaacgc tttggcatca ctgaaatgga agtaacagat gaagtcttcc gcagcaaata
     2401 tgctcgtcaa tttgaccaag cagaaaatcg tatgcactca atcaaagcga tcatggcagc
     2461 aacattaggt aacttgttta tccctcgcgt ttaagcataa ccacattcat ctcacgaaaa
     2521 aacgagtcag cattgaggga aaatcacaaa acgctggctc gttttgttat ctagaaaatt
     2581 cggtagaact gaagaaagga cgaatgaaat ggccaaacga aaagtagttg tcgctttagg
     2641 gggcaatgcc atcttgtcta cggatgcaag tgcaaaagcc caacaagaag cgttaatgga
     2701 aacagcaaag tatttagtga aatttatcga acaaggagat gaattgatca tctcccacgg
     2761 aaatggtccg caagtcggta acctattgat tcagcagcaa gctgctgatt ctgagaaaac
     2821 accggcaatg cctttagata catgtgttgc aatgacagaa ggatctatcg gttactggtt
     2881 acaaaacgcc atgggcgaag tactgaagga aaaagggatc gataaagatg tggtttcttt
     2941 agtcactcaa gttattgtgg atgaaaatga tccttcattc aaaaatccaa gtaaaccagt
     3001 cggtccattc tacacggaag aagaagccaa agaacaaatg aatgctgact ccactgtgac
     3061 attcaaagaa gatgctggac gtggatggag aaaagtagtc gcttccccta aacccatctc
     3121 gattaaagaa gcacgtgtca tcgaaacgct ggtcgatcaa ggagttatca cagtctctgt
     3181 tggtggcggt gggattcctg ttgtagaaac agctactgga ttagaaggac gagaagccgt
     3241 aatcgataaa gattttgctt cagaaaaatt agccgaaatc atcgatgcgg atttgctgat
     3301 cgtattgaca ggagtagaca atgtgtatgt caattaccaa aaaccagatc aaaagaaatt
     3361 agaaactgtt actgtttcag aaatgaaaca atacattgat gaaaagcaat tcgctccagg
     3421 aagcatgctt ccgaaagtag aagcagctat tgcatttgtt gaagcaaaac caaacgctaa
     3481 ggcaatcatc acttctttag aaaatatcga gaatctttta gcttctgaag aaggaacgat
     3541 tatcgtagct gattaatttg ttaagtcttt cgtatttacg aaatgattta tagctatcca
     3601 ggtggtattt cttctccttt aggccatctg gattttt
//
LOCUS       DACEKH010000151         3628 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEKH010000151 DACEKH010000000
VERSION     DACEKH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.158"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1157
                     /locus_tag="IW662_002590"
     CDS             <1..1157
                     /locus_tag="IW662_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ0867383.1"
                     /translation="RAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDR
                     KELASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGH
                     QEQTNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHS
                     LPNTDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEE
                     REVQYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAK
                     RGTMENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMT
                     IHSIRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(1606..2898)
                     /locus_tag="IW662_002591"
     CDS             complement(1606..2898)
                     /locus_tag="IW662_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0867384.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(3172..3432)
                     /locus_tag="IW662_002592"
     CDS             complement(3172..3432)
                     /locus_tag="IW662_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867385.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT         1192 a          689 c          607 g         1140 t
ORIGIN      
        1 aacgagcgta ttttactcat gacaacttat ccatacttga gcagttcatt atgcaattaa
       61 ttgctggtta ctcagctgat tctgcagcta atctcctgag acaagatcct gtgtttaaag
      121 ctgttttaga taggaaagaa ctcgcttctc aatcttcact ttcccgattt ttagatcgac
      181 tatctgagga gaatatccat gaacttcaag ctttgaacca agaacttatc gataaagcac
      241 gcctcatccg taatgatacg gaattaatca ttgatttaga ttcgacccat tcagatacat
      301 ttggtcacca agaacaaacg aattataata cacactacca aacctatggt taccatccat
      361 tggtagcttt tgacggattg actggtgact tcttaaaagc tgaactacgt tcaggaaatc
      421 aatatacatc aaaaggcgta aaggagtttc tcacaccttt attagagcac tataatcact
      481 ctctaccaaa cactgacatc ttggttcgtg gagacagcgg gttcgctaca cctggtgtgt
      541 atgattcgtg tgaatcaaaa aagagtcatt atgttattcg actgaagaat aatcgtagac
      601 taggtcaaat agctgagaaa tcagtacttt atggcgataa tcaaaagtgg gaagaacgag
      661 aagttcagta cttctcgacc acttatcaag cacaatcctg gtcacaaagt cgtcgcgtgt
      721 gtatacgctc aacacgtgaa gcgggcgaac tactctttcg acatgaattt atcgtgacga
      781 atctatcaga aaatgtttct cctgatacca tcttttctat ctatgccaaa cgtggcacaa
      841 tggagaactt cattaaagaa gcgaaagctg gcttttactt tgacaaaaca gacagtcctc
      901 gctttttgga gaatcatgtc cgaatgatgc tcagcttgat agcttacaac ttagtcaact
      961 tcttaagaac gattggcttt gaagaagtcc aaaagggaat gaccattcat tctattcgat
     1021 tgaagtttct gaaagttgct gggaaattag tccaaacggg tagacgagtc tatctcaaat
     1081 tatctagtta tcatgtgtat cagaatgaat tctacagggt ctttgctcgc ctgaggcgag
     1141 ccagtcaatg gatctaatcc aacaatctaa cgattttttt actgggaagt tatccaggga
     1201 gaagtatgct caaaatgcct tacacccaaa atttattcct caatattttt gaacagtgaa
     1261 tgtttgaagt aactttttag tcaaaatcag ttgataggga tattagtgat acatatttca
     1321 ataaaatgtg ccaaagaatt aaagctatga attattcagg atagaataag atttattcag
     1381 tcttagatgg atttatagca actttttcgt aacaacttta aaaaatacga aaagaaagag
     1441 ctgttttact ctgactacgt tgctaaattg tcgtttaatc attcatttaa accaaatctt
     1501 tttttataga attcgtttta atcctcctga atccaaaaac tatgaaaacc gtaattaaat
     1561 agttaagagt ttttaaccac cataattctc tcggctattt tgaaatcaga taaattttga
     1621 tcggacacat agttgaataa acctaccgtt gttacggaag gtaaatcgca tacttttcat
     1681 tctagaggag aaggtctcgt tgatcattaa ctgattaagc accatagccc agttttgaat
     1741 tcgaccacct gaccacttcg cgtgtaattc tttcgtgcgt aaataaagta gttttaggag
     1801 tgcattctca ttagagaacg ctcctttttt tgtgactttt ctgaagctgg agtggacact
     1861 ttcaaccgca ttggtagtgt acataatttt tcgaatggca ctaccataat caaatagttg
     1921 ttcaacatgt gcaaagttcc gtttccagac atctacagca ccagaataat gagaccaccg
     1981 attttgaaaa ctgtcaaaag cagcatgtgc agcgtttaga gaagaagcac cgtagaactt
     2041 tttcatatct cggcagactt ccttatagtc cttacttgga atatagcgca atgcatttcg
     2101 aacaagatga acaatacagc gctgaacaat taccgatgga aagatcgctt ttgcgccttc
     2161 ttcgaggcca gaaacaccgt ccatcgaaat gaaaaagaca tcttcgacac cacgtgcttt
     2221 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattta accataatcc
     2281 taaaatctcc ttatttcctt tgagatcata gccaagaatg gtgtatacag catattcttt
     2341 ggcttcataa ttttctcgta aagtaacata catacaatca acgaatagaa aggcataaca
     2401 ctttgctagg gggcgggctt gccattcttc caattcagga aggacagcgt cagtgatatc
     2461 tgaaatcatt tcatgggaaa tatcaaagcc ataaatagct tcgacggttg cggcaatatc
     2521 tcgttgactc attcctcgtg catacatgga aagaaccttc ccttcgatgt cggagacatc
     2581 tcgttttctc ttaggaatta actctggttc aaaggaagct tcccggtctc taggaacatc
     2641 aatagctact tcaccaaaac tggttttaag cgttttagtt ccatagccat ttcgacggtt
     2701 atcgtgttcc ttaggctctt tagaatgggc atcataacct aaatgattat tcaattctcc
     2761 ttgaagcatt ttttcaaaaa ggggcccaaa cacatctttc aaagcatctt gcatgtcatc
     2821 gacagattca ggttgatagg cattcagaat ggattcagct aacttttcgg catcaggatt
     2881 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attgtagaaa aagaaaaacc
     2941 gcgaagcaac cacctgccta ggattaatgg ttacttacac ggtttatatt acactctcat
     3001 cattaaaaaa gtatctttcg atattactaa acaatgaaat tatctatttg aaatttaaat
     3061 ctgataatat attgagtgtt ccaatgaggg ctttgatcct attatccata tctttttcag
     3121 ttttaatatt gatacacatt ataagaaaaa gaaacttttg gttaatttat ttcatcccag
     3181 tgcaaaatat aaaaaagtaa aatagaccat aggcaataga ttaacaatta atataataca
     3241 agcaaatatt ctattcattg tatttctgct ttcaaaaagt tctaatattg aaagtataat
     3301 tccacaaatg cctataataa gtatgggaat aagattagaa aataacaccc cgagccaatc
     3361 tgtgaaaatt actatattta ctaatatccc tattaacata cctatcatat ttattaaatc
     3421 aatttttttc attagatatt tctcctttat taaatctgaa gtacttatat taaaataatt
     3481 ctaaactatt tacctcaaaa agtgcaataa attatttctg aacaataaaa tgatagtcaa
     3541 aacaaaaaaa tattgttaat cgttcagagc catccgagag tgtaaaatat tttgtgtaaa
     3601 tgaaaaaatc catacaaaaa aggaaggc
//
LOCUS       DACEKH010000152         3530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEKH010000152 DACEKH010000000
VERSION     DACEKH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.159"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(27..530)
                     /locus_tag="IW662_002593"
     CDS             complement(27..530)
                     /locus_tag="IW662_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0867386.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(653..1417)
                     /locus_tag="IW662_002594"
     CDS             complement(653..1417)
                     /locus_tag="IW662_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ0867387.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSADQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1524..1799
                     /locus_tag="IW662_002595"
     CDS             1524..1799
                     /locus_tag="IW662_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ0867388.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            2515..3465
                     /gene="yidC"
                     /locus_tag="IW662_002596"
     CDS             2515..3465
                     /gene="yidC"
                     /locus_tag="IW662_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0867389.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         1071 a          722 c          668 g         1069 t
ORIGIN      
        1 atatggtcta ttattggttt tacctgttaa atcgaggatt ctttcccaaa gtaacgttgc
       61 catctcttac gcatacttgc agacatgggc tgagccgctt ctttgactgc gcagtatttg
      121 tccacgaatg tcacaatata tccttcttta tacttaggcg gtgcgatcgt tgcaccccac
      181 atgtgcttca aaatgatatc tcgctcaaga tccgacaaat cggtgatttt ttctgcattc
      241 tttacagcaa ttcttggatg gatatacgca tgtgtcccct cgtcaaattt cgtcgtacgc
      301 caatcatagt agaacagatc atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca
      361 ccccattttt ttgcgagcag ataactatag taagaaacac tgatggaatg ctccaaacga
      421 gtggaatgaa catgctgtgt gtaattcgct aattttttca ctgcttctgt ttctaaaaga
      481 tcttgtacat aagacatata ttcttcgtct tcacgccatt tttctgtcat aattatcgat
      541 ctgccacctt tttgaatttc attttcttct gaaattgatc taagtatacc accagtgttc
      601 ggattattct agtcgctcta gataaaatag ctaaagctta atagttcctt aatcataaag
      661 aaaaatgata aagaacgatc cctgctgcga ctccgacatt caatgattcc gcatttcctt
      721 tcattgggat atatacgttc tgatcagccg aagacaagat ttcctgactt actccctgac
      781 cttcgtttcc catgattata gcgaaatcct ctgttttttc tactttgtct aatgcatgtg
      841 cttcttcatt taattctgta ccgtacaccg taaacccttt gcctttcatt acagggatca
      901 cttctgccaa atctttttgt aacacaggca aatgataatt gcttccctgc atgcttcgca
      961 gcacctttgt actataaata tctgccgttc ctttacctaa taaaacacca gccataccaa
     1021 acgcatctgc cgtacggatc atcgttccca catttccagg atcctgtaca ttatctagta
     1081 aaagccagcc tcctgaaaaa tctggcgtat cttcagatgt taacgtcatc actgctaaga
     1141 tcccttgcgg tgtaggtaga tctgacatag cggtcatgac ctcatctgaa actagaaaag
     1201 tagaaatctc tttgtttaca gccagccagt ctccccattc tttcatacct ctctgtgtca
     1261 caaaaagttc ttttatttct gcatcagacg acgcagcttc ttcgatcaga tggaaccctt
     1321 ccaaaagata ttctgccttt tcttctctgt atttcttttt atgtagtttt tttaattctt
     1381 tgattcttgg attttttact gattggatct ctttcacatt cctcacctca actgattata
     1441 tcatggaata ggtgcttgag cttgtttcat cctaaaaaaa tcggtatcat tgatttataa
     1501 aacaggaaag aaaggtgacg aacatgcgca aagttaaaat gaatgtccaa ggacgtgtcc
     1561 agggcgtggg atttcgctat atgaccaaga tggttgctga tcagttaggc gtgaccggaa
     1621 cggtaaaaaa cgaagacgac ggctcagtca caatcgaagc aatcggaaat gacgacatca
     1681 tccaaaaatt tatcgaagaa gtcaagaagt ctccgagccc aagtggacga gtgcagtatg
     1741 tggatgtcca agaagatccg atgattgagg aacgaaagaa atttgatgtg gtgggataag
     1801 gttgtttttg aagcgctttg cctcgagtat taaggagaaa cattttccaa atagcttctc
     1861 atattttgaa atgtaatgac ttaataccga gaggcagctg ataaaacacc gtcaataagg
     1921 ttgtgaaaaa gctggtctgc atcaagcatt aaggactggt tgtgaaaaat agctctccat
     1981 atttttcaac aggctggact taatgccgta gatgcaagct tttgaacacc gtcgataagg
     2041 ttgcgaaaaa gctgatctgc atcaagcatt aaggactagt tgtgaaaaag tcgcttagtt
     2101 ttgagtatta aggaaaattt tgtgaaaatg gctttccaca tttttgcata aatttgactt
     2161 aataccaaga aactgacttt tgaacatcgt cgataaggtt gtttttgagg cactttgcct
     2221 caagtattaa agacaggtag taaaaaagct gatctataac ttgcaataaa tttgcttaac
     2281 ccatattttc cagacagaac aaaaaagttt ttagtttttt aaataagaaa gaaacacttg
     2341 gaatcactct tttctttcta agtatttctt tcttgatttt ttgtcttttt tcaaaatttt
     2401 tcgctacgaa aaagaacgaa tatttatatg aaatttctat gaagttgatt gaattccatg
     2461 cgctttttta gtatagttaa gtttgtgtaa aaattataga agaggaagta ttctatgaat
     2521 aaatggaaaa actggttatt gggatccgga cttgtcagta tcctgctctt tttatctggc
     2581 tgtgtgcgga tggattcaaa cggaaaccct gacacttcag gaatcgttta tcgagtactc
     2641 gtccatccta tgggccaagc gattacatat ctagttgaga atttcaattg gtcttacggc
     2701 tgggcagtta ttgcaatgac tgtgatcgtt cgtatcatca tcttgccact aggtatcagt
     2761 caatcgaaaa aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc acaagttgaa
     2821 gcagcacaac aaaagctaaa agctgcaaca acacgtgaag aacaaatggc agcacaagca
     2881 gaaatgcagc aagtatatcg cgaaaatgga ttgagtatga ccggcggaat cggctgtctg
     2941 cctctattga tccaaatgcc gatcttctct gccttgtatt ttacggctcg ctacacagaa
     3001 ggtatccgtg aatcatcttt ttacggcatc gatctaggct caccaagtat ggtgcttgtg
     3061 gccatcgcag gggtcgctta tctgcttcaa ggatatattt caacaatcgg tattcctgaa
     3121 gaacagaaaa agacgatgcg tacgatgctg atcgtctctc ctgctatgat cgtcttcatg
     3181 tcgatcagtg cgccagccgg tgttacactt tactgggtag tcggtggtgt gttcagctgt
     3241 ctgcaaacat tcatcacaaa cgttataatg aaaccgcgtt tgaaagcaca agtggcagaa
     3301 gagttgaaac aaaacccgcc aaaacaagtc gttactccaa gaaaagacgt gacagaatca
     3361 gcaaagccag ctgacaacaa acaagcaaca aaacaaatca aacaaccaaa aaacagtaca
     3421 aacaacaaag gccgcaacgc aggaaagcag cagaagcgga aataaaaata tatgacaaaa
     3481 aactgtagac aaataaaaaa atttctttgt tctacagttt ttttgttaca
//
LOCUS       DACEKH010000153         3462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEKH010000153 DACEKH010000000
VERSION     DACEKH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.160"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            62..340
                     /locus_tag="IW662_002597"
                     /pseudo
     CDS             62..340
                     /locus_tag="IW662_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
     gene            483..704
                     /locus_tag="IW662_002598"
     CDS             483..704
                     /locus_tag="IW662_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0867390.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            720..1562
                     /locus_tag="IW662_002599"
     CDS             720..1562
                     /locus_tag="IW662_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867391.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1625..3427
                     /locus_tag="IW662_002600"
     CDS             1625..3427
                     /locus_tag="IW662_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ0867392.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
BASE COUNT         1249 a          480 c          709 g         1024 t
ORIGIN      
        1 gctatctttt tctattattc taccttattt gatagtagat ttaaaacttt gcaacagaac
       61 caaagaactc atcaagctgg ctaataaggg tcaagattat gaacatctag cagatgagat
      121 tgatgagctg agagacaagc gacagctcct tttagtagaa gatgcctccc tcagtggcga
      181 gaacgagcga atcaatgagc tgattgaatt tatccgcaag aacaaattcc gtaccttaga
      241 gtacgatgat aagcttgtaa ggaagataat ccagagcgtt acagtctatg aagaacactt
      301 cgtcatatcc ttcaaatctg gcatcgaaat ggaaatatga aaaccagaag taaatagccc
      361 atgattctac agtagagtcg tgggttttct tttgcttgct gattataata atattttaat
      421 aactattgtt attataaacc aaatgattta taatattctt agcgtaaact tggaggtgct
      481 gtatgactac ggcagaaatg attaaagaac tgtgtgagca aatgaatata agtgtttccg
      541 aacttgctag acgtattgga cagactccac agaatttcaa taagaaatta caacgagaaa
      601 cggtaacctt agatgagttg aaagcgattg ctgatgtgct aggtgtcaag tttgtgcagg
      661 cttttatatt gcctgatggc aatgaaataa agataggtaa ttaggaggtg taatttgtat
      721 tgaaaatcat aacaataaat gatgttgagt atgctgtatt tgctgctaat gaaggtactt
      781 ccaaaccaca acctcatatc atagaaacga aatctggtac tattccagaa ggaaaacaac
      841 tgagtttatt aaaagaatat cttaagcaga atgatatttc cccaataaaa ggagcaacaa
      901 catattggtg cattgacaag gttcttaagc ttgattcaag taaagaaaaa actataagtg
      961 aaacaatcca taaacaaaaa tacttatctt taactgaaga aaatattgag aaacagcata
     1021 agtttgtagg tgcaagctct aattatggga aagaaggtct aataatacat gatgttttga
     1081 atgcatttcc tcttcataat gatttaaata ctattgcgat gaagattgcg gtaattgatg
     1141 taacaaattc aacacatctt tctcaatata agagcaggtt gtctctatat gatttggcta
     1201 aagtaattct ggagattcca aattttgatg atcggttggc aaaaggagat cctcagttga
     1261 taaatattat cgcaaggaac attggtgcag ttaatatgtt ctcatttgca tccaagtact
     1321 gtacatatca taatgtggaa gtgtgtggga gagatgatta ctccatcttt gatggtatcg
     1381 ttaaaaatac tcttccacac tatatccaag gactaactac gaataagatt gatacgtggc
     1441 gaagaagttt tgattacgag gcatttaacg aatgtgttgg aaaactactt gatgaaaata
     1501 atatacatat tccttttaga agaagaaagt tagatcactt cttgtggtat gcgaataggt
     1561 agaatataag aaaaaggcat cgtaaaagtg attctggata aaattaatgg gggaggaaat
     1621 aatagtggga ttatttgatt ttctaaagaa aaaacaagga aaggttagta aacacgttga
     1681 ttatcaactg gcttctagtc tatttcgtac tggtaaagtt tttgctgaaa aattggaagt
     1741 ggacatatca aatgtagatg agtttcgtaa aacttttatc gcttttgatg tggaaacaac
     1801 tggcctaaat tcttcttcag atagaattgt agagattggt gcggtgctat ttaaagaagg
     1861 agttccaatt caatcattcg gaactcttgt caatccaaaa gttagaattg cagcctcagc
     1921 ttcggcaata aataatataa ctaatgaaat gctaaactct gctccaaatg agcaggaagt
     1981 ttacccccag ttggtagaat ttttaggaga tgcactagat ggaaaaatca ctatgtgtgc
     2041 acacaatgca agtttcgatt ttggattttt aagtaacaca ttttctagac ttgggtatga
     2101 tgctaaaatt aagtatgttg atactctgag tctctctaga aaacatataa aaggcattgt
     2161 aaatcataag caagagactg tagcggaata tttcggatta ataaatgaca ctgcccatag
     2221 agcggaatca gatgcagata tatgcggaaa gattttttgg ggtgttttag atattattga
     2281 ggagtcgttg gaagaacaaa aaaagcaaat agaaaagatg actccagaca taaaagaact
     2341 tgaagcttgt gcttttattc aagatattat tgagaaaaaa ggatgcgaca ccagttggat
     2401 caggtatcga aaaaacagta ataattatgt cgatgtaact tgtctatata cattcttaaa
     2461 gtttaagttt gctaagaagg gaaattacat cattattaga aaagatgcta caaatggaat
     2521 tgatttacct atagaacctt gtactgtaag tgaaggcggt acaacaaatg tgcgagtcta
     2581 ttttcgtagc ccctttgact taacgcctct ttcaaattat attttcagtg cttatagcga
     2641 ttgttataat tctatgaatg aatatatgag tgttagtaat tacactaaaa aagaagcaga
     2701 gcaaagcctt agtatgctga aatctatttc aaaatcagcg atgaaagagt tattattcga
     2761 tgcaaaaaat cgtggatatg atgagactga agttaaccta aatattgaaa aagtgatatc
     2821 tagagaagat gtaattatta acgggttgca taatcgagtt tcattaaaca agatacgaaa
     2881 tatagggaat tgggagaaag gatttgatgc aggttatcca tattgggaac gtggagaaac
     2941 tgctaggaaa aatggcaaag tagatgaagc tattgcactt tttgataaag ctcgatataa
     3001 cggctatgat gctcctgcgc tatatgattc ttatgctaaa gcttatcgaa aaattaagga
     3061 ttacgataat gaaattctca ttcttgatga aggaattatg cgtaaaacac ggcacgatgt
     3121 tggtacttta gaggctcgta gggataaggc aattaagcta ttatttgcta aacaagaggc
     3181 agagcggata gctaaggaaa aatctgattt tctaaaagcg aacaaaaaag aggatgcgat
     3241 tagtgagcct agcaagaagc gtggaagaca gattattcaa atgactgatg atggaacaat
     3301 aataaaagaa ttcgatacta tagcttctgc tgtaaaagaa gttggcgtta actcaaaaag
     3361 tattagggat gctgcaaatg gagttcaaaa acatgctgga ggatactgct ggaaatacaa
     3421 agattagcag ataatctaag ataacctagg atcttcttaa ta
//
LOCUS       DACEKH010000154         3392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEKH010000154 DACEKH010000000
VERSION     DACEKH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.161"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            210..476
                     /locus_tag="IW662_002601"
     CDS             210..476
                     /locus_tag="IW662_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0867393.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            503..802
                     /locus_tag="IW662_002602"
     CDS             503..802
                     /locus_tag="IW662_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ0867394.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            976..1527
                     /locus_tag="IW662_002603"
     CDS             976..1527
                     /locus_tag="IW662_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ0867395.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1673..1966
                     /locus_tag="IW662_002604"
     CDS             1673..1966
                     /locus_tag="IW662_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867396.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1959..2255
                     /locus_tag="IW662_002605"
     CDS             1959..2255
                     /locus_tag="IW662_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0867397.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2330..3328
                     /locus_tag="IW662_002606"
     CDS             2330..3328
                     /locus_tag="IW662_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0867398.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1278 a          461 c          667 g          986 t
ORIGIN      
        1 atttagaaaa acttactaaa caaagagata tgaaaaactt taataattgt gtagttttga
       61 taattataac tgaattaact ttatttacaa atatatatta tatccatgaa gcccaatctt
      121 ctttaataga agattgggct atttttatgg caaaggaggg aaaagaaatg tttcaaaata
      181 aaggataacg ttatgtcaca aagggagtaa tggacagttt gccggtggaa ttacaagctt
      241 tatgttggaa tctaatcgat caaaacgttc aaaagcaatt tcctttagac tatcttcaaa
      301 tttttgaatt ttctacggaa aaaggaaatc aaaagttggt tcatcgtcaa gaagaaccgg
      361 aagaaagaaa agagtatctg atttcaccta agcttcgttt gaaatcagtg tctcaaaaaa
      421 tatgggccat tgattcgggt gagtatcaaa cgatgttatt accagaagaa tactagtatc
      481 taaaagaaag gaacgaaagg acttgttaga aacggctatc gaagtactgg aaaagtgtgc
      541 acagctggtc actgcttctg aagagtgggg ctatgaatcc gttacgatgg aaaaagagga
      601 aattgaaatg ggtatgcttc ctgaagatgt tcatctacca cgtttagtga tgacacatct
      661 ttatatttat tgcgcaccag aagatggaaa agactatgta gtttatttca ttacagatat
      721 taccagccag cgagaatttg ttcgtgggtt actggtagaa ggacgattgg tttggagtca
      781 gattggagga acggatgaat aagatcgatt atttgagaat gattgcgaaa tgtttagaag
      841 atatccttac cgcgattcat caaattctga agaaaaaagg agaaagaata tgaaatattt
      901 tcaactagta ttgacactta taacggcaat ggtaattgaa gtgcttgaac aaaaaatagc
      961 ataggagaaa aagagatgat tgaaaaagaa ttagtgacca cacaaacaga agtttggcga
     1021 gatcctcatc atacccatcg ctatgttttc aaacgacaat gggcgatcaa aggaaaagaa
     1081 gcggaagata aattagcagt agtcattact attcgtccaa cagatacaga accatttacg
     1141 aatgatttga gtatgttatt aatcgaaaag aatatccgac aattaggatt tactggtttt
     1201 attgctgtga atcttttttc gtacacaaaa gcgaaaagtc ctcgggtatt tcttcgagga
     1261 aatgatgaac aatctttgga aatattaacg actgttttta cagaaaagaa aatcaatacc
     1321 attatttttg cttgtggttc gattacagcg acgagtcaag tggcttatga acaagcaaag
     1381 actatttacg atcaattgag tgcgaaacaa aagaagatga ccaaagtatt gataaatgca
     1441 gaaggtaagt tatcccaccc actaagtatt catgttcgta atgaatggca attggcagat
     1501 agtcaattat tatttcaaga aaaataacat tttattttct catgttactt ataagtagtt
     1561 ttacatgctt tttgacttta aaaatacatg tgatgataca tgttatatga attatcaatg
     1621 tacccagtaa tatgctattt tcattatgta aaaaataaca gggggtgtgt agatggagat
     1681 tgatgaaact tgtttgagta actcttataa aagtatgaat gacttttttg aggaaatgga
     1741 actggattca gaattgctct atcaatttta tcttgctttt cgagggcaac agatttcttt
     1801 tccaatgaaa atgtacgatc gtgagttagt tagaaaaaga atcgaaaaca tgatagaaca
     1861 ggaaaaaact gtagatatcc gtcaattgac agaagtttat ggtttctctg caagatggat
     1921 tcaaaaagta attaaacaaa aagaaaggag acgagtacat ggctaagctg attgtattag
     1981 gtggtttaat ttacggtgga tattggttat ataaatatat gcttgcgcat ccgaatatag
     2041 ataaaggaaa agtaattaag acagttgggg taatcatagt tggactcata gctctaacta
     2101 agaaaaataa tactaatagc ccaaaaataa tgagaactta tcgctgtaga ggatgtggaa
     2161 gggaaatttc tactagtttt aatccatctg agagtcctca aggaagagac cgctgccata
     2221 cgggctatcg acattattgg gaaagaatcg attaaaagat taccatttag atcaattaat
     2281 tgtctgatag aataagtacg tgagggagaa attgaaaggg ggaaatataa tgaaaaaatg
     2341 gctgttatta ctaataggaa tgggaatttt attcactggt tgtacatctg agaatacgag
     2401 gactaaagcg actatagaaa ctgatacaac aattgagact aaaaaaagta aaacgcaatc
     2461 aactgaacaa actacaacag aaatgaagca atctgtggaa acaagtgcaa gttcaatcac
     2521 tgaatcttct tctgaagagg ttaagaatac tttgtgggat acgaataaag cttccaaatt
     2581 agaaactttt gtcacacaat ggggaaagac attaggtcaa gaatataaaa gctacaatct
     2641 tcagaataat gtaagtctgt atggcactcc tttaccacaa gccgtgatta atggcgattg
     2701 gaaaatggca atcaatgaag caccagttac cgtacaatgg tcagaagatg gaactggtca
     2761 tgcagatttc aatttagtag cagtttattc tgatgtagaa actggagaat atttagggca
     2821 gcatgtttac tttttcggtt ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa
     2881 tcaaggtaat gaaaataatt atctgtactt taacgaaact caaaatcaac agttaaaaca
     2941 aggtttcagt gatattgtga atggacaaac tcctcaaaca ccagtagtag aagaatctac
     3001 tcaacaagca acaactacaa cgacacaatc tacaagtagt tcaggaattt caagttggga
     3061 agctgctaaa gaatctgtgc taaataataa agctttatgg ttgtatgatt ctcctgataa
     3121 aaatgtatca aatactaatt taattagcac aaatgaatat atgaaagatc taatgcaaga
     3181 tgaaaatgga agctactatt caattaatat tgtaacggat gatgatggaa acacgaccat
     3241 ggctggtgga aaacaatttc gtgtttatac agatggaaga atttctcaac gtattggaat
     3301 gactaacaat tttcaaatga ttaaataatt gaaaatatta tttatcaaag aaaaactgat
     3361 tttgagaaaa attatttaat tttcttagaa tc
//
LOCUS       DACEKH010000155         3376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEKH010000155 DACEKH010000000
VERSION     DACEKH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.162"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            37..588
                     /locus_tag="IW662_002607"
     CDS             37..588
                     /locus_tag="IW662_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ0867399.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1264..1530
                     /locus_tag="IW662_002608"
     CDS             1264..1530
                     /locus_tag="IW662_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867400.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1588..2100
                     /locus_tag="IW662_002609"
     CDS             1588..2100
                     /locus_tag="IW662_002609"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0867401.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2097..3134
                     /locus_tag="IW662_002610"
     CDS             2097..3134
                     /locus_tag="IW662_002610"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0867402.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            3294..3376
                     /locus_tag="IW662_002611"
                     /pseudo
     CDS             3294..3376
                     /locus_tag="IW662_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         1242 a          576 c          669 g          889 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa attatctaaa gagaattaag ttttgattat gtaataagtt
     1021 ttggtactaa aaaagacttg atctaattag accaagtctt ttgttagtgt tatattaata
     1081 acaaaataaa aataagaagt cgctcactcc ccgaccaaag tttgtgagca aaataaacag
     1141 tcgatgtttg tttcttaatt gtatatcttg atattaaacg atttaagatt attcttcaag
     1201 atatatattc gcaaatgtaa ttggaaaagc gacttcttaa acaaaattaa ggagtcgctt
     1261 tttatggcaa aaataacgaa aaatgagcaa gaaaatttgg tggaaaatta cacaaaaaaa
     1321 ccggcgtact ctaatagccg gttagacgca catttcgtgt gcacttctaa tccgaaattg
     1381 agctttgacg ctatgacgat agtcggaaac cttaataaaa acagcgctaa aaagctatcc
     1441 gattttatga gtttagatcc acaaattaga ctttgggata tacttcaaac aaaatttaaa
     1501 gctaaagcac tacaagaccc cctatgttag gctagttgtc gtataggata ataagttata
     1561 ataaagacta tacggaggat gattaccatg acaaataaaa acaatgaacg ttactctacc
     1621 gaactgaaag cgacagccat caagaaaatg atgccacccg aaaatagggg gattcgccaa
     1681 ttaagtcaaa ctttgggcat tccagaagcg actctttgtt cttggcgtaa aaaagctaga
     1741 attcaaggga atactacacc cggaacgaaa aaaaatgtcg aaaaatggtc aagtgaagat
     1801 aaatttttaa ttgtgatgga atcattctca atgaaccaag tagaacttgc ggaatattgc
     1861 cgaaagaagg gcttatttaa agaacaaatt gaggcttgga agaaaacgtg cttatctgcg
     1921 aatgaacagg aagaaaatcg aacgagagaa cttgcaactg aactaaaaga agagaaaaaa
     1981 caagcgcgtc aattagagaa agaattacgt cataaggaaa aagcattagc ggaagccgct
     2041 gctctcttat tactaagaaa aaaagcacac gcgatttggg gggaccaaga ggaagaatga
     2101 tttcatcttc agatcgcaaa caagctgttg aacttattca agaagccaat cgcaatgggg
     2161 cccgtctcac ctatgcctgt aatgagctga atattagcgt tcgaacctac gaacgatgga
     2221 cgcgtgaagg tacgatagcg catgatcaac gccctttggc gaaacgtcct gtacccaaaa
     2281 ataaactgac ggatcaagaa cgcgaaaaaa taatagaaac cgtttcaaaa aaagaattta
     2341 tgaatttgcc acctagccaa attgtaccaa aacttgcgga ttgctcgatt tatattgcat
     2401 cagagtctag cttctatcgt gtattgcgag aaaaaaatat gcaacatcac cgtggccgaa
     2461 gtcaagtttc acaaaaacgc ataccgccaa gtcaccttgc gacgaagcca aatgaagttt
     2521 ggacttggga tattacttgg ctaaaaggtc caataaaagg actcttttac cggttatatc
     2581 tcatcattga tttatttagc cgtaaaatgg taggctggga gatttgggaa tcagaagaag
     2641 ctcgtcatgc cgaaaaactt attaaacaga cggtgcttaa ggaacgtatc caaggacagc
     2701 cgttggtttt acactccgat aatggcagcc ctatgaaagc cgcgactttt caagtcttac
     2761 tcgaaaaatt aggaattcaa agttcttatt caagaccacg agtgagtaat gacaatcctt
     2821 attcggaagc ggctttccga acgttaaaat atcgaccaga atatcccaat aacggcttta
     2881 aaacaataga tgatgctcga aaatggacga aagagtttgt cgattggtat aacgattctc
     2941 atttgcatag tgggattcaa tttgtgtctc ctgcagcgtg tcatgaagga cgacatctcg
     3001 ctattttaga gaaacgaaag caggtttacg aggacgctaa aaagcgccat cctgaacgct
     3061 ggtcaaaagg tgttcgaaat tggactccac atcaatcggt ggcgttgaat cctttaaaag
     3121 aaacagaaag ttaacaggag cataatcttt caagattatc aaataaccct cgtcacccaa
     3181 taggggaaaa agttattctt cccctattgg ataacgaggc caaccgacca tagggaggtt
     3241 ctgctttttg ataggtggaa aataaaaata cgacaactat attgacaaac accgaccatc
     3301 gtccagtaaa gagacgcaat aaattctatc gaagtttacg cactgcctct acaacgatta
     3361 aaggcatgga agccat
//
LOCUS       DACEKH010000156         3369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEKH010000156 DACEKH010000000
VERSION     DACEKH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.163"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW662_002612"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW662_002612"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ0867403.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IW662_002613"
     CDS             complement(2260..2511)
                     /locus_tag="IW662_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ0867404.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IW662_002614"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IW662_002614"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ0867405.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          920 a          739 c          626 g         1084 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaa
//
LOCUS       DACEKH010000157         3322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEKH010000157 DACEKH010000000
VERSION     DACEKH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.165"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            279..788
                     /locus_tag="IW662_002615"
     CDS             279..788
                     /locus_tag="IW662_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0867406.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            881..2043
                     /locus_tag="IW662_002616"
     CDS             join(881..1157,1157..2043)
                     /locus_tag="IW662_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0867407.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2323..3303
                     /locus_tag="IW662_002617"
     CDS             2323..3303
                     /locus_tag="IW662_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0867408.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
BASE COUNT         1188 a          532 c          656 g          946 t
ORIGIN      
        1 tttttttatt tgtctcgctt aattatacaa acggcgtgat aaaaaacgag aaatgaatta
       61 taggtttaga cctttatcat ttctcgtttt attttattgt atcaaggctc tatcataagt
      121 ggtaaaatct acttttaaat cacatgtaat cattgataag tccagtgttt tagcggataa
      181 tcagcttttt atttattttt tcttataaat ttgataaata gaaattagta tagccctaaa
      241 caaattattt gtgttttatt gtttagggct atactatatt ggtacaagga atagaaagag
      301 gtgtttatgt ggttgataaa attcagttta atcaagttat agaagaattc atcaaagtaa
      361 ataatttaat gacccatatt caaaaacgac caatcagaat aactaatgat ctaaaactca
      421 gtacgagtat gattcatttg attgatgtga ttggaaatta tcctgaaact agtattacgg
      481 aattagctga tcgattaggc gttaccaaag gagctgtatc ccaacagatt cccactttag
      541 agaaaatgaa tcttataagc atttctcaaa aaaaaggaaa taagaaaact aaacttgtat
      601 cgttaacaaa atatggtcga aaggtgtacg aatcacataa ttatttacat gaagagcttt
      661 atagctcaat aaatgaagcg ttagaatcat tctcaccaga ccaaatttca accattaaac
      721 agattctaga aaaagtatct tatacaatca atgaatatca agtaagtctg acaagaaagg
      781 aagattgaca tgaaaaaaat gatttttgaa tcggctacac tagactagac agaaaaaata
      841 aggtgtgtaa aataaatgaa aacacactgg aggaattttt atgtcaagac gtcaacgtag
      901 aacctattca aaagaattca aacaacaagt cgtcgatctt tatctcgcag gaaagccccg
      961 agctgaaatc attcgagatt atgagctgac tacctcggcc tttgataagt gggtcaagca
     1021 agcgcaaact actggctcgt ttcaggaaaa agataacttg acccctgaac aagtagaact
     1081 aattgcatta cgaaagaaaa ataaacagct tgaaatggag aatgacattt taaagcaagc
     1141 ggcgctgata ttgggacgaa aagacaagta attgatacca ataagcacaa atactcaata
     1201 tcagcgatgt gtaaagtgtt aaacatctct tgccaaacct attattatca agccaaacca
     1261 gttgaagatg aatcagaact ggaagaactc gttcaagaag aatttattcg caatcgaaaa
     1321 gcttatggta ccaggaagct gaagcagtgc ttggccaatc taggtcttca agtgagtcaa
     1381 cgcagaatcg gccgtatgat gaaacagcgt ggcttgcgtt caacatacac attggctcat
     1441 ttcaaattcc attcttctac ttgtaacgaa gccaagaccg ccaatattct agacggaacc
     1501 ttcctacagg aaaagtcatt agaagcgatt gtaaccgacc ttacctatgt tcgtgttggg
     1561 aagaaatggc agtatatctg cctgattctt gatttgttta atcgagaaat catcggctat
     1621 tcatgcgggg agaagaaaga tgcgacgtta gtcaaggaag cttttgcaaa gattccgtac
     1681 tcgttgaata atgtacaact ttttcacacg gatcgtggaa aagaattcga taaccagacg
     1741 attgacgaaa ttttatgggc ctttaatatc acgcgttcct taagcaaaaa aggctgcccc
     1801 tatgataacg ccgttgcaga atccacctat aaatctgtca aagtagaatt cgttcatcaa
     1861 taccgctttg agacattggc taatttgcga ttagagctat ttgattacgt ccattggtgg
     1921 aataccatcc ggctgcacgg cacgttaggc tatgagacac cgatcagttt tcgacagcag
     1981 agattggcgc agcgaatcct tgataatgag ctcggatgtg ataccggtgg cgaggcacct
     2041 taattatttg aatgagcttc tgccttagag aatcagattc gaggaggctc gttgtcaagg
     2101 gcagtcggaa caccagcagc aagcttacgc aataccttat aatttttgtc aaaaaaagtg
     2161 ttgccattcc acatatatct ttaaacagct attgccagat aaaaataaat gatattatat
     2221 atataaatac ttatccgaaa aggggaatga gagagtcaag gttcttggaa tgtcgaatag
     2281 tcgagtaggt agattttcta tctacaacct tgttgtttaa aaatgaaaaa aattggaatt
     2341 agagaagttg cgaaagaggc aaatgtttct cctacgactg tgtcgagggt attgaatgat
     2401 cgtggatatt tgagtgagga aacaaaaaga aaagtttttg atgccatgaa aaaattagaa
     2461 tactatccta atgaactggc gagagcactt tttaaaaacg aaacatatac cgtaggtttg
     2521 atttttccta caattactaa tccatttcac gctgagttaa ttcaagatat cgagttcgag
     2581 ctttccagca ggggatataa agtgttgctg tgcaatagct taaacaatcc aacaaaagaa
     2641 aaagagtact tgacgatgtt gagaaagaat caggttgatg gaattatagt aggaacgcac
     2701 aataaagata ttgcggatta tgatattcca gggctaccta ttgtagcgat tgatagaagt
     2761 cttggtgaaa atacagttac agtatcttgt gataattaca ggggtgggga attagctact
     2821 caactacttg taaatcatgg ctgtcagaat atactgtgta ttcggggaga tagtaagata
     2881 aagctacccg cgaatagtag gactttagca tataaagatg ttttgaataa attcatgctg
     2941 gaaccgttag tcctagaagt accatttgta aaagaaatca atgaaaaaaa tgaaataatt
     3001 aagacatatc tggaaactca tccaaatatt gacggggttt ttgcaggaga tgatttattg
     3061 gcttctttgg cgattaacaa cttaggaagt tcgggcaaaa gagttccaaa agaggttaaa
     3121 gtgattggat ttgatggagc tcaacaaaca ttgatttata atccagaatt atcaactatt
     3181 caacagccta tagatcaaat atctaaagta gcagtaaaaa aattattgaa tatgattaag
     3241 ggcgaaaagg agacggatga actttcactt ccagtaaagc tcataaaaaa aaatacagca
     3301 taagttaaat cggcgaaaaa tt
//
LOCUS       DACEKH010000158         3219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEKH010000158 DACEKH010000000
VERSION     DACEKH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.166"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            49..1437
                     /locus_tag="IW662_002618"
     CDS             49..1437
                     /locus_tag="IW662_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ0867409.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1775..>3219
                     /locus_tag="IW662_002619"
     CDS             1775..>3219
                     /locus_tag="IW662_002619"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0867410.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1184 a          505 c          583 g          947 t
ORIGIN      
        1 tcaaaaaaaa accaaccgaa agatataaaa gatattgtag atacacgaat gtataaagaa
       61 cgtgatgact taaactatac gcttattatc aataaagatg gaaactattc atggcgtcct
      121 ctcgttctac tacatcctat tctatatgta gatttggtta attgtctcac taaagaagac
      181 cattgggagg agttaaaaaa gaggtttagt gaatttaaaa aagataaaaa tattaactgt
      241 tattctatac ctgttgaagc ttccaaaggt tcaaaaagga cagatcttgg tgaaacaata
      301 ctaaactggt gggaatattt tgaacaaaaa agcttagctc agaatattaa ataccaatat
      361 tgcatgttta ctgacatcgc tgattgttat ccatcaattt atactcactc tataacatgg
      421 gccatgtatg gtaaagaaaa agttaaaaat gatgaaaata agtgttcggg ctcacttggc
      481 gataaaatag ataaaaaaat atctggaatg caacacaatc aaacaaatgg tatccctcag
      541 ggttccgttc ttatggattt tgtagcagag atcgttctag ggtatgttga tttgagatta
      601 tcgaaagaat taaaaaaatt agaaaattta aaagattaca aagtactcag atatagagat
      661 gattatagaa tattttcaaa taatcgtaat gatttggaac aaattatgaa agtgttatca
      721 gaagtcttat atgatttaaa ttttaagtta aattctagta aaacaaaatt atcagatgat
      781 attatcatag attctatcaa aaaagataag ctctattggg aagaaattaa ggcaagtata
      841 agaatgaatg ccacaaactt agatggtgat agagaaattc gttatagtat aaatttacaa
      901 aaacatttat tggaaataaa aaagttagca gataagcatc ctaattcagg ccaattgaaa
      961 aaagcacttt ctgagtttaa taaagaacgc attattaaaa tcacttcaaa gccgaacgat
     1021 cttttaccac ttattggtat cctggtagat atcatgataa ataacccggg ttctttgccg
     1081 cagtgtgtct taattatagg gcagttatta aatttcgaaa atgagagtaa ggctagagaa
     1141 atagttgaaa tgattataaa caaatatgct ttaaagccaa atactgaatt tatgatgata
     1201 tggtttcaaa gattgaccac catttttgga gaaatgtttg atagtgtttt atcatccgaa
     1261 tttgtatcta aaatcaaaga tcctgtctct aatcagatgt tttctgtaga ctggctaaat
     1321 gaagatgcta agaaatcttt ttatgaaggg accattaaag acgaagagaa atatattaaa
     1381 atgagtaaga aaatatatat tactgaattt gatgatttta atgattatcc tttttaagtg
     1441 aaaatttaga atgaatataa aaaacattac aatctaattt ttttaaaaaa atgttatatc
     1501 ggaagtaatc tcttgatttc tatactataa gataagaaaa ccactattta acaaggttat
     1561 tcgaacctta ttttaagtta cttattattt aatgtatcga aaaaggtgaa ataggtttgt
     1621 tataataaga attaagtaag acatgaattg tctaagcaaa tgtagcttat ccatttcatt
     1681 aaaatacata tggtgaaaaa acaagttcaa atagtaacta attttttaaa aaactgattt
     1741 ataaaaaata caacacgaat gaggaaaata accaatgaca gaacaaatcg aaaaaaattc
     1801 aaaaagctta tatcaggcac tgtggaattc cgcagatatc ctgcgttcaa aaatggacgc
     1861 taatgaatac aagagctatt tactaggctt ggtcttttat aaatacttat ccgacaacat
     1921 gctgcggtat gtctccgttc tattggagga agaaacggac gacctaaata aagcacaaga
     1981 cctttacgtt gaagcattta aagatgcaga tataaaggat gatctcttgt atgaactaaa
     2041 agatgagttt tcatatacca tcgcacccgc attgaccttt accgcgcaag tggcagccat
     2101 tcatgatgga tcttttcaat tagaagacct ggtacaaggt ttccgtgaca ttgaacaatc
     2161 cagcgaaaac tttgagaact tatttgaaga cattgatctg tattctaaaa aactggggtc
     2221 aaccccacaa aaacaaaata aaaccatcgc agatgttatg aaagaattag aagggttaaa
     2281 catggcgggg catgctggcg atgtcttagg cgatgcctat gagtacttaa ttggccaatt
     2341 cgcatctgaa tccggtaaaa aagccggtga attctatacc ccacaaccgg tcgcaaaatt
     2401 gatgacacaa attgttttac aaggaaaaga agataaaaaa ggcttttctg tttacgacgc
     2461 gaccatgggt tcgggctcgt tacttttgaa cgctaaaaaa tattcccatc aaccgggaac
     2521 catttcttat ttcggacaag agttgaatac gtctacctat aatttggcgc gtatgaacat
     2581 gattttgcat ggggttccca ttgccaacca acacttgcat aatgcggata ccttggatca
     2641 agactggcca acagaagagc caacgaactt cgatggggtg ctcatgaacc caccgtattc
     2701 tgcaaaatgg tcggctgata aaggcttctt agatgaccca cgcttctctg cctatggcgt
     2761 attagcccct aaatcaaaag cggactttgc tttcttatta catggctact atcatttgaa
     2821 agatacgggc gtcatggcca ttgttttgcc acatggggtg ctctttagag ggggcgcaga
     2881 agggaagata cgcaaagcct tgttagaaaa tggtgccatt gacaccgtca ttggcttacc
     2941 tgctaacatc ttctttaata cgtccattcc aacgaccgta attatcttga agaaagaccg
     3001 tgcgaaaaaa gatgtgctct ttattgatgc gtcacaagat tttgaaaaaa taaaaacgca
     3061 aaatacgcta cgagattacc atattgacgc tattttagag gcctataaaa cccgtacaat
     3121 taaagacaag tatgcttatg tcgcagaatt tgatgaaatt gtggagaatg attacaactt
     3181 aaacattccc cgttacgtgg atacctttga agaagaaga
//
LOCUS       DACEKH010000159         3209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEKH010000159 DACEKH010000000
VERSION     DACEKH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.167"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            116..976
                     /locus_tag="IW662_002620"
                     /pseudo
     CDS             116..976
                     /locus_tag="IW662_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
     gene            988..1815
                     /gene="panB"
                     /locus_tag="IW662_002621"
     CDS             988..1815
                     /gene="panB"
                     /locus_tag="IW662_002621"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAQ0867411.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1830..2678
                     /locus_tag="IW662_002622"
     CDS             1830..2678
                     /locus_tag="IW662_002622"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAQ0867412.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2688..3083
                     /locus_tag="IW662_002623"
     CDS             2688..3083
                     /locus_tag="IW662_002623"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAQ0867413.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
BASE COUNT         1246 a          326 c          642 g          995 t
ORIGIN      
        1 gatattatga taaattaact atggatgata tttgaaaaaa acatactgta tatttgattt
       61 aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact tctttatgga
      121 ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt cattcgggaa
      181 gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagcg aagactcgtc
      241 attagcagct tcgaatttta catctagtaa acaatattta aacttaagag aattagtaga
      301 tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg tatggcaaga
      361 aattaaaaat tattaaatca aaaacaaatt aatctgccat tgcagtggtg caatatcttc
      421 agatatcttt tcaaatatac aggactatgg tgcttatggc tattctgtcc atcctatgtt
      481 ttcaatatca gataaatata attcatacaa aaaattaaaa aaatctttta taacaattga
      541 aggcgatgaa aaatatgcaa agtttttatg tgatttcttc aaaaagttag gtaatagtac
      601 tgtaattgta tctaaggaga ataaatctct ttatcatgcc gcatcagtag tatctagtaa
      661 ccttgtgcta ggacttatta atacaagcgt tacatatcta attcaatgtg gatttactga
      721 aaaaatggca attgaagctt tatatccatt aattgaattt aatattagaa atataaaaga
      781 aaaaggaatt atagaaagtt tgacagggcc agttgaacgt tgtgatattt caacaattaa
      841 aggtcactgt gaagttttat cagatgaaga cagagcttta tatatgctat tatcaaaaaa
      901 tgtattagag atagctatgt taaaaaatat taatagagat tatagtgaat tagaaaagta
      961 tttaggagag agttaggaga gaaatagatg aaaaatacag cagttacatt taaagaatct
     1021 aaattaagaa atgaaaaatt aacaatgctt acagcttatg actattcaac agcaaaaata
     1081 attgatgaag ctgggataaa tggaatatta gtaggagatt ctcttggtat ggtatgtcta
     1141 ggacatgaag atacactttc agtaacaatg gaagacatga ttcatcatac tagagcagtt
     1201 acaagaggag ctaagaacac tttagttgtt gcagatatgc catttatgtc ttatcaaact
     1261 tcagtatatg attcagtagt taatgcagga agattaataa aagaaggacg agctcaggtt
     1321 gttaaattag aaggagggat agaagtatgc gataaaatag aggctatagt aaaggcttct
     1381 atacctgtta tggcgcatat aggtttaaca cctcaatctg ttaatgcatt tggaggattt
     1441 aaggtacaag gaaaggacaa ggaagctgct aaagaattaa taagagctgc taaagctgta
     1501 gaaaaagcag gagcattcgc tgtagtgtta gaatgtgttc caactaagct tgcagagtta
     1561 atttctaaag agatatcaat acctacaatt ggtattggag caggtgcagg gtgtgatgga
     1621 caaattcttg tatatcaaga tatgttgggg atgtatagtg attttacccc taaatttgtg
     1681 aagaaatatg ctaatttatc agaagagatg aataaagcat ttacaaaata tatagaggaa
     1741 gtaaaagatg gagtttttcc aggtccagaa catgggttta aaataagtga tgatgtttta
     1801 gaaaaattat attaaaaggg tcaagaaata tggttatatt taaaacagtg aatgatgtta
     1861 gaagtcaagt aaaagagtgg aaaaaacaag gcttaaaggt aggattagtt cctactatgg
     1921 gatatttaca tgaaggtcat gaaagtttaa ttagaaaagc atcaaaagaa aatgataaag
     1981 ttgttgtaag tatctttgta aatccaactc agtttgggaa aaatgaagat ttaggtagtt
     2041 atccaagaga cttagaaaga gatatagaag tatgcacaag aggtagagca acagcaatat
     2101 ttaatcctga agttgaagag atgtattgtg acaatgcaag tacttttgtt aatataactg
     2161 gattaacaga aggtttatgt ggagctagta ggccaataca ttttagaggg gtttgcactg
     2221 ttgtaagtaa attatttaac ataataccag cagatagagc ttattttgga gaaaaggatg
     2281 cacaacagtt agctgtaatt aaaagaatgg ttagggacct taatattgat atagacgttg
     2341 tagggtgtcc aattataagg gaagaggatg gactagctaa aagttctaga aacacatact
     2401 taagtttaga ggaaagatct tcagctacta ttttaaataa atctttaact ttagctaagg
     2461 aagctttaaa taatggagag agagacagct taaaaataat tgagataata agtaaaaata
     2521 taaatactta taatttggca aagattgatt atgtagaagt tgttgattct ttatcattac
     2581 aaagagtaaa ttatattgaa aaatcagttt tagttgcaat agcagtattt ataggaaaaa
     2641 caagattaat agataatttt acatttgaat tacaataggg ggctataatg aaagttacaa
     2701 tgttaaaagg aaaaatacat agagctacag ttgaacaagc tgagttagat tatgtaggaa
     2761 gtattactgt tgatatgaat ttattagaag cagcaggaat atatgaatat gaaaaagtac
     2821 aaatagttga tgttaataat ggaaatagat ttgaaactta tacaatagct ggagagagag
     2881 gaacagggat gatttgcttg aatggagctg cagctagatg tgtatcaact ggagataaaa
     2941 taattttaat ggcatattgt gaattaaatg aaaatgaggt taaggatcat agacctaagg
     3001 ttgtttttgt cgatgataat aataaagttg aaagagtaac atcatatgaa aaacatggaa
     3061 ggttatcaga tataattctt taggttttgt aatgttattt atatatagct agactattat
     3121 tgaaatattt attgattact aattggatag atgtcaataa agtagacaca aaaagagaag
     3181 atacagactg tagacaaagt ccattttat
//
LOCUS       DACEKH010000160         3168 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEKH010000160 DACEKH010000000
VERSION     DACEKH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.168"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(862..1203)
                     /locus_tag="IW662_002624"
     CDS             complement(862..1203)
                     /locus_tag="IW662_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0867414.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1203..1433)
                     /locus_tag="IW662_002625"
     CDS             complement(1203..1433)
                     /locus_tag="IW662_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0867415.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1720..2058
                     /locus_tag="IW662_002626"
     CDS             1720..2058
                     /locus_tag="IW662_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867416.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2263..2502)
                     /locus_tag="IW662_002627"
     CDS             complement(2263..2502)
                     /locus_tag="IW662_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ0867417.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2478..2825)
                     /locus_tag="IW662_002628"
     CDS             complement(2478..2825)
                     /locus_tag="IW662_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ0867418.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         1004 a          578 c          489 g         1097 t
ORIGIN      
        1 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
       61 aataatattg acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac
      121 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
      181 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      241 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      301 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      361 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      421 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      481 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      541 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      601 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      661 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      721 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      781 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      841 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      901 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      961 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
     1021 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
     1081 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1141 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1201 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1261 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     1321 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1381 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1441 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
     1501 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
     1561 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
     1621 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
     1681 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
     1741 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
     1801 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
     1861 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
     1921 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
     1981 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
     2041 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
     2101 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
     2161 tatatgcagc ttatgagtat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
     2221 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
     2281 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
     2341 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
     2401 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
     2461 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
     2521 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     2581 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     2641 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     2701 agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc
     2761 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     2821 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     2881 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     2941 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     3001 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     3061 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     3121 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgact
//
LOCUS       DACEKH010000161         3035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEKH010000161 DACEKH010000000
VERSION     DACEKH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.169"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(136..1284)
                     /gene="tnpB"
                     /locus_tag="IW662_002629"
     CDS             complement(136..1284)
                     /gene="tnpB"
                     /locus_tag="IW662_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0867419.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1301..1705)
                     /locus_tag="IW662_002630"
     CDS             complement(1301..1705)
                     /locus_tag="IW662_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ0867420.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            2064..2318
                     /locus_tag="IW662_002631"
     CDS             2064..2318
                     /locus_tag="IW662_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0867421.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            2319..2636
                     /locus_tag="IW662_002632"
     CDS             2319..2636
                     /locus_tag="IW662_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0867422.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          962 a          509 c          535 g         1029 t
ORIGIN      
        1 cctgacggtt cttaacgctt gaagtgggag cttcttggga atagagcata cttgatagcg
       61 tatttcttta ctaacagcgg tgtctcttat ttaccaagct atccccgtag ttcctacggt
      121 tcttgatgat tggatctaag ccaatcccat agaatgtagt cttagacctt cggtcagaat
      181 attgatacta gcattgatat cacgatcatg atgagtatga caaacaggac aagtccattc
      241 tcgaatttca agagatttct tgccatcttt atgtccgcac tctgaacaaa tctgactaga
      301 cggaaaccat ttatctactt tgatgatttt tcgtccatac cagtcagcct tgtattgtaa
      361 tttagtcaca aactttgacc aagaaacatc agaaatactt ttagctaatt tatgatttcg
      421 caacatacct tttgtgttta agtcttcaat acagataata tcgtgatttt tgatcatttc
      481 tgtactcaac ttattcagaa aatcagtacg ttgattcatt actttttcat gcaatctagc
      541 tactttgcgt ttttgttttt gataattttt agcttcaaat agattggtac ctttattttt
      601 agccaataaa gcacgcctag acaatttgcg ttgttcacgc tttagtttct tttccatttt
      661 ggacgtgaat ttattattat caattttttg tccatcagaa agaatcgcaa agtccataat
      721 acctaagtca ataccaattg tggaattagt tttaggtaat tcgataattt cttctttaca
      781 caacaaagaa atatagtatt taccgctaga atgacgtgat attgtagcag atttgataat
      841 cccttttggt tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat
      901 gaatttacta tcaatcaaag caattgtacc attttgatta ttcgttgtat aactctgaac
      961 aggatttttc ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt
     1021 gtatgcttta tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa
     1081 aaacggaaat tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc
     1141 atttttaact tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg
     1201 gacacaacca aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa
     1261 tttgtatgct tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt
     1321 gattttggat atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa
     1381 agcttttaga ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt
     1441 ttatcaatct tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag
     1501 gttgagcttt gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa
     1561 tgtgatacga ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat
     1621 caatcacttg tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg
     1681 aatgattatt tgtatctaat tccatgttat atcaactcat ttcttgtata gactgattgt
     1741 aacatgggac aaaataaaaa ggcaatacgc cttagttgtc ggtcttcgag ttaccaacgg
     1801 taaccctcat accgaacggc attcatcacc cacctataga ggatgggcga cttctgccta
     1861 attctgttaa atggatgtta acggaagtgt atggtgagta gttcactcgt attttgatat
     1921 cgtttgtata tgttttcagc gttctaagtg gcttattttg tccaataata atatggagtg
     1981 gaatccctta agaaacaccg ttggcaattg ttgttataac atgttataat ttattcatca
     2041 aaaactaaaa ggagtgtgat aacatgggaa aagaactaaa ggttagaaag atcggcaata
     2101 gcgtgggagt gatccttcca agctctcttg gattaaaaag cggagacatt atccaagcca
     2161 aacaagaaga caacttattc atattagata ctactcaaat agcaaaagaa cacgatcgca
     2221 aattgattga agaaagtttc caagactttg agaaaggttt aacagtttca gaaattgaaa
     2281 tggtaaaagc tttcggaaaa tacggctgga gtgaatagat gaaagagtac acgctagttt
     2341 attctaaaag ttttcagacc agccttgaaa cgataataga agaatgggaa aatgagcttg
     2401 ctctttctcc agccagcata aaaagatttg taggtgcgat ccagaaggct ttagaactaa
     2461 ccaaaaattt tccaactatg catgaggaag tttccgaaat ctatggaatg gatatcccaa
     2521 catatcggat cgtgatcggc aaaagctatg ccttatttta tcggatcgac catgaaaaaa
     2581 aagagatact tgtaggacgt attttccaat ctaaacaaat gaaactaggc ttttaacaat
     2641 aattaggaaa aagtcgctca tcctctacag gtgggtgatt aatgccgctc gatatggttg
     2701 ttaaccgtcg gttactcgaa gattgatata taagacttag taccttttta ttttgctcca
     2761 tgttatggcc gaaggtctaa agatacaatc attagtctag agaaaaatta agaaccgtag
     2821 gaactacggg gatcgcttgg tgaataagag acactgctat tagtgaaaaa ataagatatc
     2881 aagtacgctc tattcccaaa aatttcgtga ctttactcat gagaggttca agtagtatat
     2941 ttttaatcaa aattgacgta acttttaata aatactacct aaataaggta gtatttattt
     3001 ttgtgtttta aaagaggaag tccaatatat ctatg
//
LOCUS       DACEKH010000162         3013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEKH010000162 DACEKH010000000
VERSION     DACEKH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.170"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..288
                     /locus_tag="IW662_002633"
     CDS             <1..288
                     /locus_tag="IW662_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0867423.1"
                     /translation="KTLFRLLMQYRSTGYYVVRIEEFRSLMDIPEYYQMGNIDQKVLN
                     PALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKISLRNWLEE"
     gene            complement(425..841)
                     /locus_tag="IW662_002634"
     CDS             complement(425..841)
                     /locus_tag="IW662_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867424.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(844..1128)
                     /locus_tag="IW662_002635"
     CDS             complement(844..1128)
                     /locus_tag="IW662_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867425.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1829..1924)
                     /locus_tag="IW662_002636"
     CDS             complement(1829..1924)
                     /locus_tag="IW662_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0867426.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2577..>3013
                     /locus_tag="IW662_002637"
     CDS             2577..>3013
                     /locus_tag="IW662_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0867427.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKT"
BASE COUNT          962 a          479 c          565 g         1007 t
ORIGIN      
        1 aaaactctat tccgactgct tatgcagtac cgttctactg gttattatgt agttcgtatt
       61 gaagagttta ggagcttaat ggatatccca gaatactatc aaatgggtaa cattgatcaa
      121 aaagtattaa atcctgcatt acgagaacta aaaaaatact tcaatgatct taaagtcaca
      181 aaaataaaag caaaaaaggg caataaaatc gccaaattag aatttacttt ttcaagccta
      241 acgtcagatt tgccaaagat ttctctgcgt aactggttag aagaataaat aaaaaagaac
      301 tcgcccccct tacattaagc aagtagacaa gtcccttaca accgactaag attgctagta
      361 cattctagca tattgtatgg ggcttctaaa taaaaaacag ataggaataa ctcctatctg
      421 ttttttatcg ttttagaaaa tttgttgggt cgaaatttct agaagtgact gtttgactgg
      481 tttctttttg atgattcttt ggtcgatttc gatttgatgg atttagttta gcttcgattt
      541 tttctttcac ttcttgatat actttagaat ttgtgccaat aagaacgcct gtatctttta
      601 gatataaatc agcaaattct gcgatgattt cagtcatgat ggaaatttct tttcccatag
      661 catttagttt tgcctgattg ctggtagtgg tcgtgttggt atcgggttca taactcatac
      721 ataaaaagcg aatggcttcc gagtagttat tgatatctgg atttttttcg atgatggaat
      781 ctaaaacgtc aacatgacta gggtacaaag taacatgttt ttggacagaa gtttttctca
      841 tggttatatc attcctccga taagcatttc aatgatttga ttatttgtgt agacatactc
      901 gcttaatatt tgagtatttt ctgtttggat agcaataaat ttatcgagca ataaatcgaa
      961 ttggtctttt ttgtaagtcg taagttgacg gtcaaaatca ctttcgatta aaacttttag
     1021 atatttattt cgactccatt tttctcctgt tttattcgtg agttcttggc aacgtttgtc
     1081 tattgttgca acaaactttg gatcaatatt ccgaactaaa atgtccataa aaaatctcct
     1141 tttgcaatta tgtataattt atgtatacca tacaaatact atatttgtat agctgttttt
     1201 tgccaaaatg ctatacataa gctacccacg atggcgaaaa agtgatatca aatttttttc
     1261 tgagaaacgt gataggcaca gcctatctac ccactttttt tagaaagtgg gtacacgttt
     1321 ccttatgctc tttaaaattc gggggggagt gctgcacgtg taatcgataa ccaaatttaa
     1381 cagacgaaaa attgttggaa tataaaaata tgacacccga aagaaaacga tcgttggaaa
     1441 aattaatgca actagtgaac gaagacggcg gaatttcaga cgcagctttt gacgcatgga
     1501 gcgaatttca tggagaatat aattaagtgg gtttaaatgg ctctaaaatc gcttctaaga
     1561 gttttttagc ttggaccaat gaaaatacac caaaaactaa ataaaacgtc ttaaatcgaa
     1621 aaatacgagc tctcagagcg actttatttg taaatgttgc atataagaaa taattacgga
     1681 ttttagactt ttgtaggata tttttgaaat aaatatttat ttctgttata atttgattgc
     1741 aaataagtga acacgaaaca cacgaaatcc ccagagaggt cggattctct ggggcttttt
     1801 ttgtgggctg agtcgcctta attaagtttt acttcttgtt tcggttattc aaccagtaac
     1861 tgaacaaagc gcttaaaagc ccaacaagaa gggagcaaat aaagttagtg aacacgaaac
     1921 acacctcctt ccagtcgcaa gactgtcgga taggcgacaa tcaattatac aataaaaaag
     1981 caaaaataag cctaagtttt ttattgcctt ggggttgtta gggcttgccc tgaccgcctg
     2041 taaggcgctt tagaacgaag gacatagttt ttatcgaatc ttgtcttaga acggcttaga
     2101 cgagctctca ggacgccaca aaggagtccc taatgaatta tttttggcga aggagcctaa
     2161 aggcgactgc tgcaagtaaa atgtgagagc gtagcgatca ctccttctta cagcgaccat
     2221 agggagctag attggcagtt taatatcatg cgcgaacctg tttattttaa aaagtgggtg
     2281 attttttggc gggaaatttt gctcgcgcgc gcgcgtttat tttatttatt ttttaaagat
     2341 tttttagaac cctttttagg gggcagttga gccttactct cccaacggat acagaagggt
     2401 actaacaact ttttatctga gtactaacaa ctttttatct gagtactaac aactttttat
     2461 ctgagtacta acaacttttt atctgagtac taatatcctt ttgtataata acaaccaacc
     2521 tatttacgag ttggttgtta aaggttatta tttatttgta aggggggaag aaaggaatgg
     2581 atgaaaaaac ggaattaaca gtcaaatatc aaaatgaatt gaatttagta ccgctgaaaa
     2641 atttcaatgc taagcagatg gatttatttt ttgcattatg cgctcgtatg aaagatcgtg
     2701 gtttgaaaaa agttagattt tctttcgagg aattaaaaga gttaagtgat tataagatga
     2761 ctgctacaaa agcctttgtc ggtgatttag aaaagctcta taaagatatg cttaatctta
     2821 cttaccgtac tgaagatgag gattctattc gctactttgt tttgtttact ggttttgaaa
     2881 tcaataaaac aaaaaagttt gttgaagtca gtgttaatcc ggacctagaa tatattatta
     2941 atggtttaac agctgaattt agtagatttg aattatctgc tttcacgtcg ttgcgttcta
     3001 cgtatgcaaa aac
//
LOCUS       DACEKH010000163         3005 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEKH010000163 DACEKH010000000
VERSION     DACEKH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.171"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(106..1053)
                     /locus_tag="IW662_002638"
     CDS             complement(106..1053)
                     /locus_tag="IW662_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0867428.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IW662_002639"
     CDS             1299..1763
                     /locus_tag="IW662_002639"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ0867429.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IW662_002640"
     CDS             complement(1825..2868)
                     /locus_tag="IW662_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ0867430.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          993 a          596 c          575 g          841 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccag
//
LOCUS       DACEKH010000164         2958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEKH010000164 DACEKH010000000
VERSION     DACEKH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.173"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(24..959)
                     /gene="murB"
                     /locus_tag="IW662_002641"
     CDS             complement(24..959)
                     /gene="murB"
                     /locus_tag="IW662_002641"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ0867431.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1032..1952)
                     /locus_tag="IW662_002642"
     CDS             complement(1032..1952)
                     /locus_tag="IW662_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0867432.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2065..2817
                     /gene="xth"
                     /locus_tag="IW662_002643"
     CDS             2065..2817
                     /gene="xth"
                     /locus_tag="IW662_002643"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ0867433.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          830 a          596 c          513 g         1019 t
ORIGIN      
        1 cttattttaa gttccattac ttattaattg gctaaacttc tgcttgtttc tgatattgct
       61 tgtttttctc caatgatccg cacttctgtt tctaaagaca catcaaattt ttctttgatt
      121 acttcttgga tgtgcgcaat caattcaata taatctgtcg ctgtcgcatg atcgatattg
      181 acgatgaatc cagcatgttt ttcagagact tgtgcaccgc cccatttcag gccttgcaat
      241 cctgcttgtt ggatcagctg acctgtatag tgtcctactg ggcgcttaaa gacacttccg
      301 catgaagggt attctaatgg ctgttttgat tgacgcaatt cggtcaactc atccattctt
      361 gctttgattg cttcataatc ccctgtttgc aaactgaatc gagcttccaa tacgatagca
      421 cgtaattctt ggactttact gtgacgataa gaaaatacca tttcgtcttt tgtcaaagtt
      481 ttcaatgttc catcttcaag caaaagatcc acttcagcaa aaacatcttt gatctctccg
      541 ccatacgcac ctgcattcat ataaaccgct ccaccgacac ttcccgggat gccgcaagca
      601 aattcaaatc cagtcaatga ttctgccaaa gctgcataag tcgtatcaat cagtgaggca
      661 cctgcttccg caatgaccat cgttcctttg acatgaatct gtttcatttc agtcagcatg
      721 atgactacac cgcgaatccc gccatcttga acgatcagat tgcttgcatt tcctaatacc
      781 atccaaggta tatcatggat tcggcaataa tcgatgattt gctttacttc ttcttgtttt
      841 ttcgggaatg caaggacatc agctggtcca cctgttttcg taaacgtata gttcatcaat
      901 ggttcatcaa acaatatagt aatcgtggct aagctttcaa ctatttcttc tttattcact
      961 gaggattttc ctttcttttc tcaaacgtcc tcattattct atcacgctta atacaatctt
     1021 gccagtcttg ttcaaagttg atgacggtag tgatctttaa gcacttcctc acataattca
     1081 tgacttctta caacttcttt ttgtttaagc aattctgttt ccgatttacc taaacggatc
     1141 tgagaaaaga aggcctctac cattggctca aacccgcgtt tgactagcgt gtttgtccag
     1201 tcgccaaatt ctttcacttt ttttccatca gcatcttgaa tgacaagctg ggtcagatcg
     1261 tttacttcat aagtaccatt tttacttgtt acttgatatt tttctatatt agcgccactt
     1321 gctaaatcca tcgttaaaat agccgtttga ttttgtgttt ctaattgtaa aatggcatat
     1381 tccaaaaatt cctttgtttc tcttatctgg tattttgttc tcaaaatggg ttcatctaat
     1441 aaatacacag ctgtatcaat aagatgtaaa aacaaatcat aaaggacgaa ctctgtcgtt
     1501 tcttttgccg ctattcgatt tttttgcagg tggatcaacc gcttatctgg gatacgtttc
     1561 aattcttcta ccataggagc aaagcgccgg ttgaaaccga tcatcaaaat tatttggttc
     1621 ttatcagcta attcttgcaa ttcacgaact tcatttagat ggacactcag tggtttgtca
     1681 atataaacat ggataccatt ttctagacat tttttggcta attcataatg tacttttgtt
     1741 gctgcgtgga tcatacatgc ttcgattttt tcttctaaaa gctcatcgat cgtttcatat
     1801 agatgttgga acccgtactc tttttgaatt ctttcttttg tttctttatt tcttgttgca
     1861 aaataaaaat caagtgttcc ttgttttttc gcataagttg gtaaatatgc tttttctgcg
     1921 atattcccta caccgattac accaatcttc atctactttc ctcctggatc atttagttac
     1981 tttcatctta caccattttt tactaaaatg aggtacaata gtatcgcaaa ggtaaggaaa
     2041 ttaattcgaa ggagtgtgct tttattgaaa tttatctctt ggaacgtaaa cgggcttcgt
     2101 gccatcgtaa ataaaaattt tttagaagtt tttcatgagt tagacgccga ttttttctgt
     2161 ctgcaggaaa cgaaactcca agcaggacaa attgatttag atcttccagg ttattatcaa
     2221 tattggaatt acgcggaacg aaaaggctat tccggcacag cgatttttgc taaaaaacca
     2281 gcattgaatg ccacttacgg aatgggtatc gatatccatg atacagaagg ccgtctgatt
     2341 actttggaat attccgattt ctttcttgta acctgttaca cgccaaattc tcaaagcgaa
     2401 ttaaaacgtt tagattaccg attagaatgg gaagaagcct tttataatta tttggaaaat
     2461 ctaaaaaaac aaaaaccagt catcgtgtgt ggggatctaa atgtcgctca tcaaaagatt
     2521 gatttgaaaa actggaaaac gaatcaaaaa aatgctggat tcactccaga agaaagagcg
     2581 gctctttctc gactgttgga taacgggttt atcgatacat tccgttattt ttatccaacc
     2641 caggaaggtg tctactcttg gtggaactac cgattcaatt caaggaaaaa caatgctggt
     2701 tggcggatcg attacttttt gacgagcaaa gatttagagc caggactagc tgatgcaaag
     2761 atccatacgg acattatggg cagtgatcat tgtccagtag aattagattt gaaataaaga
     2821 ggttgtgaaa aagtcgttta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc
     2881 acattttgca taaatttggc ataataccga gaaactggct tttgaacact gtttatcgag
     2941 agattgtgaa aaagctgt
//
LOCUS       DACEKH010000165         2918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEKH010000165 DACEKH010000000
VERSION     DACEKH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.174"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            205..1173
                     /locus_tag="IW662_002644"
     CDS             205..1173
                     /locus_tag="IW662_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ0867434.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1166..2197
                     /gene="vanA"
                     /locus_tag="IW662_002645"
     CDS             1166..2197
                     /gene="vanA"
                     /locus_tag="IW662_002645"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ0867435.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2203..2811
                     /locus_tag="IW662_002646"
     CDS             2203..2811
                     /locus_tag="IW662_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0867436.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          863 a          559 c          708 g          788 t
ORIGIN      
        1 taatttttta ggaaaatctc aaggttatct ttactttttc ttaggaaatt aacaatttaa
       61 tattaagaaa cggctcgttc ttacacggta gacttaatac cgtaagaacg agccgttttc
      121 gttcttcaga gaaagatttg acaagattac cattggcatc cccgttttat ttggtgcctt
      181 tcacagaaag ggttggtctt aattatgaat aacatcggca ttactgttta tggatgtgag
      241 caggatgagg cagatgcatt ccatgctctt tcgcctcgct ttggcgttat ggcaacgata
      301 attaacgcca acgtgtcgga atccaacgcc aaatccgcgc ctttcaatca atgtatcagt
      361 gtgggacata aatcagagat ttccgcctct attcttcttg cgctgaagag agccggtgtg
      421 aaatatattt ctacccgaag catcggctgc aatcatatag atacaactgc tgctaagaga
      481 atgggcatca ctgtcgacaa tgtggcgtac tcgccggata gcgttgccga ttatactatg
      541 atgctaattc ttatggcagt acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat
      601 gatttcaggt tggacagcga ccgtggcaag gtactcagcg acatgacagt tggtgtggtg
      661 ggaacgggcc agataggcaa agcggttatt gagcggctgc gaggatttgg atgtaaagtg
      721 ttggcttata gtcgcagccg aagtatagag gtaaactatg taccgtttga tgagttgctg
      781 caaaatagcg atatcgttac gcttcatgtg ccgctcaata cggatacgca ctatattatc
      841 agccacgaac aaatacagag aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt
      901 ccacttgtag atacctatga gttggttaaa gcattagaaa acgggaaact gggcggtgcc
      961 gcattggatg tattggaagg agaggaagag tttttctact ctgattgcac ccaaaaacca
     1021 attgataatc aatttttact taaacttcaa agaatgccta acgtgataat cacaccgcat
     1081 acggcctatt ataccgagca agcgttgcgt gataccgttg aaaaaaccat taaaaactgt
     1141 ttggattttg aaaggagaca ggagcatgaa tagaataaaa gttgcaatac tgtttggggg
     1201 ttgctcagag gagcatgacg tatcggtaaa atctgcaata gagatagccg ctaacattaa
     1261 taaagaaaaa tacgagccgt tatacattgg aattacgaaa tctggtgtat ggaaaatgtg
     1321 cgaaaaacct tgcgcggaat gggaaaacga caattgctat tcagctgtac tctcgccgga
     1381 taaaaaaatg cacggattac ttgttaaaaa gaaccatgaa tatgaaatca accatgttga
     1441 tgtagcattt tcagctttgc atggcaagtc aggtgaagat ggatccatac aaggtctgtt
     1501 tgaattgtcc ggtatccctt ttgtaggctg cgatattcaa agctcagcaa tttgtatgga
     1561 caaatcgttg acatacatcg ttgcgaaaaa tgctgggata gctactcccg ccttttgggt
     1621 tattaataaa gatgataggc cggtggcagc tacgtttacc tatcctgttt ttgttaagcc
     1681 ggcgcgttca ggctcatcct tcggtgtgaa aaaagtcaat agcgcggacg aattggacta
     1741 cgcaattgaa tcggcaagac aatatgacag caaaatctta attgagcagg ctgtttcggg
     1801 ctgtgaggtc ggttgtgcgg tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga
     1861 ccaaatcagg ctgcagtacg gaatctttcg tattcatcag gaagtcgagc cggaaaaagg
     1921 ctctgaaaac gcagttataa ccgttcccgc agacctttca gcagaggagc gaggacggat
     1981 acaggaaacg gcaaaaaaaa tatataaagc gctcggctgt agaggtctag cccgtgtgga
     2041 tatgttttta caagataacg gccgcattgt actgaacgaa gtcaatactc tgcccggttt
     2101 cacgtcatac agtcgttatc cccgtatgat ggccgctgca ggtattgcac ttcccgaact
     2161 gattgaccgc ttgatcgtat tagcgttaaa ggggtgataa gcatggaaat aggatttact
     2221 tttttagatg aaatagtaca cggtgttcgt tgggacgcta aatatgccac ttgggataat
     2281 ttcaccggaa aaccggttga cggttatgaa gtaaatcgca ttgtagggac atacgagttg
     2341 gctgaatcgc ttttgaaggc aaaagaactg gctgctaccc aagggtacgg attgcttcta
     2401 tgggacggtt accgtcctaa tcgtgctgta aactgtttta tgcaatgggc tgcacagccg
     2461 gaaaataacc tgacaaagga aagttattat cccaatattg accgaactga gatgatttca
     2521 aaaggatacg tggcttcaaa atcaagccat agccgcggca gtgccattga tcttacgctt
     2581 tatcgattag acacgggtga gcttgtacca atggggagcc gatttgattt tatggatgaa
     2641 cgctctcatc atgcggcaaa tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg
     2701 cgctccatca tggaaaacag tgggtttgaa gcatatagcc tcgaatggtg gcactatgta
     2761 ttaagagacg aaccataccc caatagctat tttgatttcc ccgttaaata aacttttaac
     2821 cgttgcacgg acaaactata taagctaact ctttcggcag gaaacccgac gtatgtaact
     2881 ggttcttagg gaatttatat atagtagata gtattgaa
//
LOCUS       DACEKH010000166         2893 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEKH010000166 DACEKH010000000
VERSION     DACEKH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.175"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..950)
                     /locus_tag="IW662_002647"
     CDS             complement(<1..950)
                     /locus_tag="IW662_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0867437.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFV"
     gene            complement(1299..1625)
                     /locus_tag="IW662_002648"
     CDS             complement(1299..1625)
                     /locus_tag="IW662_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867438.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(1612..2400)
                     /locus_tag="IW662_002649"
     CDS             complement(1612..2400)
                     /locus_tag="IW662_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0867439.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          862 a          470 c          395 g         1166 t
ORIGIN      
        1 acaaaccaat tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt
       61 ttttgttttt gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca
      121 taagattcca gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt
      181 gcattatgaa tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt
      241 atcaagttta atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg
      301 acgaagttat ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt
      361 ctattagtgt ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt
      421 gagtccttga tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca
      481 ttgatttctc gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg
      541 tcttcctctt ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac
      601 aataaattag gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt
      661 ttcagaagac ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat
      721 tgtaacaatt tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct
      781 acccagttat ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt
      841 gcatcatttg atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa
      901 tatttttcac gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct
      961 ctttctgtag gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct
     1021 atccactata tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt
     1081 tttttatctt tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag
     1141 gtttaagctc atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa
     1201 tattacaata tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa
     1261 atatcacgat attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt
     1321 gctagtttat atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct
     1381 ataacgtctg gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac
     1441 attagaattg gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt
     1501 atgactttac ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa
     1561 ttctttttat ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct
     1621 gccatcaaaa tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc
     1681 agctgttgag ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac
     1741 ggttattgtc ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc
     1801 ggttgctgca gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag
     1861 aactcctcgt acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact
     1921 taactttatt agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc
     1981 agaaacagta aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat
     2041 ataatcatag ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac
     2101 ttgagtaaat tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc
     2161 tttatagtcc agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt
     2221 aggttggttt gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc
     2281 aatgtccact aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac
     2341 gctagtcgta gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat
     2401 taaaatatcc cccattcatc ttatattata atataattat atcataatat tgtgatattg
     2461 caatatcgta tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata
     2521 tcggttcgga tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt
     2581 actttaaaat tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt
     2641 taaacattac taaactgtct atattgttac taataattcc cttcttatag tgcattttct
     2701 tagaaaaaaa caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg
     2761 attgatatta tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt
     2821 ctttttctaa agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc
     2881 ttaaatcata aac
//
LOCUS       DACEKH010000167         2784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEKH010000167 DACEKH010000000
VERSION     DACEKH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.176"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1023
                     /locus_tag="IW662_002650"
     CDS             <1..1023
                     /locus_tag="IW662_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0867440.1"
                     /translation="NYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVT
                     VTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNK
                     KGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLP
                     DGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNG
                     SKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKN
                     RKKENE"
     gene            1157..2500
                     /locus_tag="IW662_002651"
     CDS             1157..2500
                     /locus_tag="IW662_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0867441.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2612..>2784
                     /locus_tag="IW662_002652"
     CDS             2612..>2784
                     /locus_tag="IW662_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0867442.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT         1111 a          361 c          516 g          796 t
ORIGIN      
        1 aattatgaag gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt
       61 ccagtagaag tgacagacaa tcgttctaat gcagataaat acaatcctga aggacaaaaa
      121 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
      181 ttaccaaaag gaactactta tgcttggaaa gagaaagtag atgttagcac agctggaaat
      241 aaaaaaggta ctattgtagt gacatatcca gatggatcat ctgatgaagt tgaagtagac
      301 gttacagtga cagatactcg ttccgatgca gataaatata atcctgaagg acaaaaagtg
      361 actacagact taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagatcta
      421 ccaaaaggaa ctacttatac ttggaaagaa aaagtagatg ttagtacagc tggaaacaaa
      481 aaaggtactg tagtagtaac atatccagat ggatctaaag aagaagtaga agttactatt
      541 tctgtagtag ataaaaaagc accgaataaa cctcaagttg atcctattac agagggtgac
      601 caaattgtta ctggtaaaac tgaaccaaat gcagaggtga cagttacatt accagacgga
      661 agtcaacacc atgggacagc tgataaaaat ggtaatttta cagttaaagt tcctaaatta
      721 gaggcaggga caaaagtgat agtaactgca actgatgaat ctggtaatac tagtgaacct
      781 actaatgtag ttgtttcttc caacaaaaaa gacagtggaa aaaacggtag taaaggtagt
      841 aagactgaca atcaaggtag caacagcaat caagacaaaa atagaggcaa aagccagtcc
      901 tcaaaagttt ttcctaagac gggagagagt gacagtaata tctttactat tagcggtggt
      961 ttaatactgt taggaacgtt aggattatta gggtataaaa atcgaaaaaa agagaatgaa
     1021 tagaaaatca atttcttaag tcttctctga gaagaggaag aatctttttc ttcctctttt
     1081 tgctttttta tgatgggaaa taagtgttag ttaaagatga atatactttt tatagaaagg
     1141 aaaagaatgt aattaagtgg aaaaaatagt aattattggt tcaaaccatt cagggctagc
     1201 cgcagctcga tttctaaact taagcgattc aaattatgaa gttattctaa tcgataaaaa
     1261 atcagaaatt agttacttgg aatgtgggac atcgttattt ataaaaggag aagtaagtaa
     1321 tttagaagat cttttttatc aaaatagaat gacactaaat tctgagataa aaaagttatt
     1381 tctagagaca gaagttgaca aaattgatat tcaaaataaa aagattagtt gtgtaaaaaa
     1441 agatggtaca agattctctc aagaatatga caaagttata ttagctacag gttcaaagca
     1501 aattatgcta gatataccgg ggaataattt agctaatatt ttttcgataa agaattttcc
     1561 aaatgcaaaa gagattttta aagctttgga tgatagtact attaaaaaag taggaattat
     1621 tggagcagga tatattggag ttgaaattgc tgaagccata agaaaaagag ggaaagaagt
     1681 atatcttttc gatgttgcag atagagtgtt gtcgacttat tacgaccgta gtttttcaga
     1741 taaagttgaa gaaatactat caaaaaatgg aatacatctt tgtctatcag aaagtcctaa
     1801 aaaatattta ggaagaaaaa agattgaaaa aatagtcact acaagtggta cccaatactc
     1861 tatggatatg gtcgtacaag ctataggatt tctatctaat agtccaattg gggaaaagga
     1921 actcttgaga gatgttagtg gagcatatct aactaatgaa taccagcaaa caaatataaa
     1981 agatatatat gctattgggg attgtgctac aacttttttt acgtccatta atagaagtag
     2041 tgttgatttt tctatttcta atgctctaag aacagcttat attgcaactc aacatattaa
     2101 caatcaagat tttacacctt ctggttccca atttacaaat ggcttttcta tatttaatta
     2161 caatttttac gcagcaggtt tgaatttaaa aacagcacga ttattaagtg agtctataga
     2221 ttatatagaa atagaagatt tcattagacc tgcttttttg agaaaaaata gtcgtgtaaa
     2281 attaagaatt agctttgaaa aaaatacaaa gagaatagta ggtgtacaac tttgtagtca
     2341 agaagattta tctggactat tatcaatgtt ttctcttgct atagaagaac aaattacatt
     2401 agacaaattg aaaatgttag attatttatt tcatcctagt tttagccaac cctataattt
     2461 tattttaaag gcagtaacag gattaaacat tgaaacataa tcgttactaa atgaaatcaa
     2521 gaaatatact gtattactca attcattgaa aatatttatc ataattttta atagaaaaca
     2581 ctattaaaga gaaaaaagag aggtaaatca aatgaaaaaa tctttaaaac ttgcatcagc
     2641 agttatatta atgtcccaac tccttatagc aacacctttc catgtgttgg caagcgaaaa
     2701 ttcaacatac aaaattgcaa aagaaagaac cgagacgact gaaatgagta attccgatgt
     2761 aacagcagag agtagtgaac tttt
//
LOCUS       DACEKH010000168         2778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEKH010000168 DACEKH010000000
VERSION     DACEKH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.177"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..197
                     /locus_tag="IW662_002653"
     CDS             <1..197
                     /locus_tag="IW662_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867443.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IW662_002654"
     CDS             314..811
                     /locus_tag="IW662_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0867444.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IW662_002655"
     CDS             898..1290
                     /locus_tag="IW662_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0867445.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="IW662_002656"
     CDS             1274..>2778
                     /locus_tag="IW662_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ0867446.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DACEKH010000169         2719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEKH010000169 DACEKH010000000
VERSION     DACEKH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.178"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IW662_002657"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IW662_002657"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0867447.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1065 a          420 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatca
//
LOCUS       DACEKH010000170         2684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEKH010000170 DACEKH010000000
VERSION     DACEKH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.179"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..320)
                     /locus_tag="IW662_002658"
     CDS             complement(<1..320)
                     /locus_tag="IW662_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867448.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            782..2164
                     /locus_tag="IW662_002659"
                     /pseudo
     CDS             782..2164
                     /locus_tag="IW662_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2400..>2684)
                     /locus_tag="IW662_002660"
     CDS             complement(2400..>2684)
                     /locus_tag="IW662_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0867449.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          953 a          479 c          459 g          793 t
ORIGIN      
        1 actaaaatag attttttgga aaactttgca acagaaccga aatgagtata gtatgaatta
       61 gtaatttggt tttcatctaa gtttagcttt gatttactaa aattgatatc tcgatacatg
      121 ttcaatacat ctaatacaaa tcgagaatct tctttcggaa atggctcaga aagatcactc
      181 caaatttcat catcataatg atactcgtaa ccttctctca atatttcgat ttttctttca
      241 taataagatc tgttttggtc ctcaaattta gccaataact caaattgatt tactagtgac
      301 aatctctgaa aactgtccat taaatgtttc ttcctatctt gttcattaac aaaaaattat
      361 aagcccttac aacgaatatt atatattaaa tttaattgta ggtgaacacg aattttataa
      421 atatttatgg tgttaatgac aaatgatatc cgactcctct tcatacgttc tggactattt
      481 gctaatagat taaaatttca gcatctattt ctgaccataa ataaatgagc tgccgaaaag
      541 ctgccaaatc agacttgaaa ataaaggatc aaaaactttt aagtttctga ccccgtaact
      601 cacacttcaa aatcttactt aaaaaggagt ctgaaagaaa aaaagagtat tcaaaatttt
      661 tttctaacta gctacatcac atctatgcat tgacactcaa agaaccagaa taagtcatca
      721 gaaagcaagg gggtgcggac ataatcctgg acgaaaaaat aaacttagga ggaatgtccg
      781 tgtgttttcc cacaaagagc gtgtaagggc aattcagtta tttttaaaat acgattgctc
      841 ttatgctgca accattcgag aattagggta tccgtcgatt ggtgcactac ggaaatggta
      901 taaagagtat ttggaatccg gagagctcca tcaggagcac cgaaaaaaat caaagtattc
      961 tgaagaacaa aaacgaatag cggtgaatca ttattttgaa tatggtcaat gttatgcacg
     1021 aaccattcgc atgcttggtt atcctaatag agagtccttg cggcagcggt gtgaagaact
     1081 tgcaccagga gctagaaaac tccgaaaaag tgcggtaaag ttaacacaag atcaaaaaga
     1141 agctgtgttg aaaaaattct ataaaccgca aactaatagg aaaaatttag ccgaatctgg
     1201 aggaattagt agagttactc tatatcaatg gaaaaacacg gttcttggaa aggattttcc
     1261 attgtgtatg actttaaaaa atcaggaaaa acaaaaagag ctacttttaa aggaagtaga
     1321 agacttacaa aaacaagtac accaattaca gctcgaaaaa gcattactcg aaggagcggc
     1381 tgaactgcta aaaaaagaaa aaggcgtcaa tcttctttgt ctatcaaatc aagaaaaaac
     1441 gatattgatt gatgccttac gaaatcagtt caccttgaaa gagcttcttc aacagctcca
     1501 gtttcctaaa agcagctatt tttatcaaaa acaagcactt gagaaaccag ataagtatta
     1561 caaagaacgt caaattaatt atcacgattt tcaatcataa cttctgtgcg tatggttacc
     1621 gtagaattca ccaagcttta aaaaatatgg gtaaaaagct ttctgaaaaa gttgtacgta
     1681 gattgatgac tgaggaaaat ctatttgtta aattttctag acgaaaaaag tattcttcgt
     1741 atgctgggga aatttctcct gcacacccta atttattaaa tcggaatttt aaagcaaaga
     1801 agccaaatga aaaatggtta acagatatta ctgaatttag aattctcgca gggaaaatct
     1861 acttatcacc acttgtcgat tgttacgatg gtgctattgt tagttggact attggaacaa
     1921 agccaacttg cttcactcta tgtctaaaaa gggttattca ccagataatt cagcgtgcga
     1981 aggtttcttt ggacgattaa aaaacgagat gttttatcaa cgtgactgga agaatacaac
     2041 aatcaatcag tttataaaca aattggatga ttatattcat tggtataata atcaacgaat
     2101 taagttatct ttaggagggc taagccctct tcaatatcgg aagaagcaaa gatatatttg
     2161 ttaaaaaggt ccaaaaaaaa tatccgcacc cccttttttt tgtaaaaaac tattgcacaa
     2221 tacaagtagc ctacggctat cgaaatttcc ataactttcg agcaagaact tatctcattc
     2281 aaggcttgat cttccaagta aaacaaaaac cagttaagca ctctgcctaa ctggttatga
     2341 ctaaattttc agttgtatct ttctctacca acactaaatg tcgaagagcc ttttttcgtt
     2401 tatgaatgta agaccgcttc tcgagccaat gccaaacaac cgagcgttcc agcgttgtct
     2461 tctaatgctg gggtcacgat atatttgctt aactcaggtg tttcgacata cccattgacc
     2521 aattttgcaa atgcgtcgtg gacgttttgc agcatatggc gttgtttcat cacaccaccg
     2581 ccaaaaatga tcacatctgg agaaaacatc aaggttgtat tgtaggcaca ttgagcaata
     2641 taggaggctt cgatttccca ataaggatca tcttctgcaa cctc
//
LOCUS       DACEKH010000171         2640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEKH010000171 DACEKH010000000
VERSION     DACEKH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.180"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IW662_002661"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IW662_002661"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0867450.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          948 a          461 c          584 g          647 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaacttgg agataacatc
//
LOCUS       DACEKH010000172         2623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEKH010000172 DACEKH010000000
VERSION     DACEKH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.181"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            36..197
                     /locus_tag="IW662_002662"
     CDS             36..197
                     /locus_tag="IW662_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867451.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="IW662_002663"
     CDS             210..713
                     /locus_tag="IW662_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0867452.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="IW662_002664"
     CDS             773..1162
                     /locus_tag="IW662_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0867453.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2623
                     /locus_tag="IW662_002665"
     CDS             1149..>2623
                     /locus_tag="IW662_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ0867454.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT          779 a          434 c          603 g          807 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tgg
//
LOCUS       DACEKH010000173         2539 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEKH010000173 DACEKH010000000
VERSION     DACEKH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.182"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(54..356)
                     /locus_tag="IW662_002666"
                     /pseudo
     CDS             complement(54..356)
                     /locus_tag="IW662_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1070..1360)
                     /locus_tag="IW662_002667"
     CDS             complement(1070..1360)
                     /locus_tag="IW662_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867455.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1568..2259)
                     /locus_tag="IW662_002668"
                     /pseudo
     CDS             complement(1568..2259)
                     /locus_tag="IW662_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          749 a          468 c          352 g          970 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcaacatac
       61 ttgcaaagta atactacaat tcctaacata atcgtagaat ttaataattt ccatagcata
      121 aagtgtttag aaaagaacat taagaatagt tctataataa ttcttgaact ccatgtctta
      181 taatgttcta tcgaagcacc caaatagcca agttcatcta atctacttgc ataaactacg
      241 tcatccgctc tcatagtcgg catatacata ttaaagagta aaaagatacc ccaaatccct
      301 attagtaaca agatcagttc tttgttattc gttatttttc tattatgttt gttcattttt
      361 atactccttc ttatatttcc taatagagta taacatagct ttaagcatta cttaaagata
      421 acttaaaagt tatcttttaa atacttaatt gttaaatacc ttatttgatg caaaagaaat
      481 ctacgatagt caacaaaaaa attttcatta tatccagaaa aatttttctt agttaaggac
      541 ctatggattg cagtaatgca gaattcgtcg aataaactgc caaagacata caatcatatt
      601 gcttttaaaa ttgacgaaca agatatagat tcgtttgtct caaaaatcca aatgttaggt
      661 ttaactgtcg aaccaggacg ttctcgtgtg aaaggcgagg cttcttcaat ttacttttac
      721 gactatgata atcacctctt cgagttacat accggtactc tccaggaacg tttaaaatcc
      781 tacaattcca ctacctgaga ctagtaatga ctagtctctt tttatggcct ctgatccccc
      841 cattgagcga atactaatag ggcttcgaat tgatctgttc aatctttaag caatatccct
      901 caaatgagtt tggaaagttt agtgattcat cacaaatact taaatacatg tagttatata
      961 tagattgagg ttcttgtgag cgtatgctcg tgtgggttca aatcccatcg tctgcatcca
     1021 tctttccaaa acaagcggct aggacatttt tgtctcagcc gcttgttttc tagtttattc
     1081 tttcatcttg tcggaccttt tttcttgggt atagactttt ctcatattct accacaattt
     1141 tgggtttccc taacacatga taggatctca caacaaattg ctttctatta ttataataac
     1201 ctgtaacgac taacttcatc ccttcgtcta cttcagctag gaagttcaaa ctatgggaag
     1261 caatcaaaca attgacccca tttaaactga aacggatcaa cggtctggca ctaacttgca
     1321 aagtacggat cttatctact gttccatatt ttttctccat aaccttcacc tcaataccat
     1381 tatacgaaca tacattccct ttgtaaagaa gaaactctgt tttatctttt tgactttttg
     1441 tctattacct ttcttaaaat catattggca cttcctgcga agatgaagga catgtactaa
     1501 aaaaaagagg cagcagtata gattactgtt gcttctttac taatttaggt ttacttgtta
     1561 agaaagatta tttacgcaaa tgttcttcaa caatttgctg agatttatca gctgtatctt
     1621 ccacttcatc ttttgctttt tctaactttt gaatatattg tttcaagctt gagttttcag
     1681 cagttgcttc gtctaatttc ttttgtgtat ctgttaactg atttctacat ttgttttttc
     1741 ttgtgtcagt gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag
     1801 ttttgcttgt gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc
     1861 atttttttgg ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg
     1921 tgcatccttg ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt
     1981 ttggttttct aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg
     2041 caatgtctgt tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc
     2101 tgtcaatgta tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc
     2161 accccacaaa ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa
     2221 tgcaacgaca atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa
     2281 tacttttaca tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca
     2341 tatgtaattt taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt
     2401 aaaaaatctt ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg
     2461 tgaacaaaaa caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact
     2521 gtatttatta caaaaaacg
//
LOCUS       DACEKH010000174         2494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEKH010000174 DACEKH010000000
VERSION     DACEKH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.183"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            282..464
                     /locus_tag="IW662_002669"
     CDS             282..464
                     /locus_tag="IW662_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867456.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            448..633
                     /locus_tag="IW662_002670"
     CDS             448..633
                     /locus_tag="IW662_002670"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ0867457.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            655..780
                     /locus_tag="IW662_002671"
     CDS             655..780
                     /locus_tag="IW662_002671"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0867458.1"
                     /translation="MYKRFESGYLQWPRSRKEVLLTGLSIEEKCTLKETKKGAIY"
     gene            876..2396
                     /locus_tag="IW662_002672"
     CDS             876..2396
                     /locus_tag="IW662_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0867459.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
BASE COUNT          861 a          420 c          498 g          715 t
ORIGIN      
        1 ttattgaaag attcgattaa attagttgag taaatgcttc gtacgaacat agttgattta
       61 ttcaccatac ttcccattac atcgtaaaac gctatattta agatttcaaa caactatatt
      121 aataggaaga aaacttcatt tttcctctta gttctataaa aagtgtggct aatttgaggc
      181 aaaatattaa attaaatgta agcgtgtcat aggatagtat ctcgtacgcc gattatcttt
      241 cttctataat taaactatca aatatagaag gagaaaaacg aatgaataaa aaagatattg
      301 taccagttca attttcaagt gcggctcaaa aatcgcaaat tgacaccaag ccaaaactag
      361 cttgcagaat caaatcggaa gataaggaaa tatttattta taatggaatg gataaatatc
      421 ttttgtatac cttgttaaaa gagttgaatg ccaatgctca ttgatgcaac tgcattgaaa
      481 catattttta tcgtttgtgg aaaaacagat cttcgaaaag gaattgatgg actagcagct
      541 ttcgttatag aagaatatga ccttgatgtt tacgatcaag cgcttttctt attttgcggt
      601 acgaagaaag atcgcttcaa ggctctttac taggatcaaa atggatttgt attaatgtac
      661 aaaagatttg aaagtgggta ccttcaatgg ccgcgttcca gaaaagaagt cttactgact
      721 ggcctttcaa tcgaagaaaa atgtacgcta aaagaaacga aaaaaggtgc gatatattag
      781 acgaaaacct tgttaaatca aggattttgt ctgtttttcg ttgtcataaa atggtataat
      841 agatgtaaga aaatcgctag aatggtggtg taaaaatgac aaaaatggaa tctgaacttc
      901 ttgtacaaat caagcaatta acacagttgc ttgaaatcca gcaacaggaa aatactcttc
      961 ttagagaaca aattgctgaa atgaatcgac gcttgtttgg acgaaaaaaa gaaacaccac
     1021 ccgtagatgg gcaaatcgat ttattagacg actcaacttt taatgaacca gagcacactg
     1081 gacaagaaag ccaggaaccc attacggtat cttcttttaa acgcagaaaa cgcaagggac
     1141 tcaaagcact ttcattagaa ggtctgcctg aagttcctat tcattatgaa ttaaaaggtg
     1201 ccgattgttt gtgtgaagac tgtggtcata ctttacatga tatcggtgcg acaaggctta
     1261 gagaagagcc attgtatgtt cctgcccata tagaaaaaca agtcatctac caacatgcct
     1321 atcattgcag agaatgtgaa gcattaggag aaacaaaaat caaaaaagcg cctgttccta
     1381 aaccgttgat caataatagt ctaggttcct cttcaattgt gacacagagt atttgggaaa
     1441 aatttgttaa aaaggtgccg gcatatcgac aagaaaaaga atggcatgca cttggtttcc
     1501 ctcttgatcg taaaaaaatc actaattggc atatcaaggt aagtgaaatg gttttagagc
     1561 caatctacaa ttgccttcat caagaattaa gcaatcagga ggtcttacac gcggatgaga
     1621 caagctatcg ggtattgaat actcataaag aaaagacctt ttactggtta tatgcttcag
     1681 gtaaaagcga agaaaaacaa gttgtgctct acgagcacgc aaattcgcgt tccgttgatg
     1741 agccaaaaag ttttttagaa tcatttagcg gtttcttgca tactgatggt tacgcggcct
     1801 atggaaagct gccggtaaac aatgtttatt gttgggcgca tgttcgcaga aagttttttg
     1861 aagcattggg taaacaaccg tccccaaaaa gtcatgccaa aataggatta gacttctgtg
     1921 acaagatgtt tcgaatcgaa cggaccttta ataagcttag cgcagaagaa aggtttgtag
     1981 cgaggaagga aacgttgcag cctgtgatga ctgaattttt tcattggtgt gagagttttg
     2041 cttatttgcc aaaatcgcag cttggaaagg cggttcaata cgcagtcaat cataaagacg
     2101 gacttcagat tgtgttgctt gatggacgct tggaactttc aaataaccga gcagagcgtg
     2161 ccatcaaaga gttagtgatc ggtcgaaaaa actggctatt ttctaaaagc ttaaaaggcg
     2221 ctagatcaaa tggaattata ttgagtatta tccagacagc tgttgcgaat gggttaaaca
     2281 tccgcaaata tttaaatcat ctttttacag agattcccaa tctgtcttcg atgacacctg
     2341 aagcgctacg cgcttatttg ccatggaatc agcagataca agaaatctgt aaataatata
     2401 agccacaaat aacctgattt gagtgtatca aatcgggcta tttgtgttta ggtaccattc
     2461 tatgacacgc ttacgctcaa aactactgaa ttat
//
LOCUS       DACEKH010000175         2419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEKH010000175 DACEKH010000000
VERSION     DACEKH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.184"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..1150
                     /locus_tag="IW662_002673"
     CDS             5..1150
                     /locus_tag="IW662_002673"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ0867460.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IW662_002674"
     CDS             1321..1779
                     /locus_tag="IW662_002674"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ0867461.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IW662_002675"
     CDS             1938..2408
                     /locus_tag="IW662_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ0867462.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          810 a          454 c          455 g          700 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaa
//
LOCUS       DACEKH010000176         2402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEKH010000176 DACEKH010000000
VERSION     DACEKH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.185"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            209..2071
                     /locus_tag="IW662_002676"
     CDS             209..2071
                     /locus_tag="IW662_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0867463.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2071..>2402
                     /locus_tag="IW662_002677"
     CDS             2071..>2402
                     /locus_tag="IW662_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0867464.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          867 a          353 c          466 g          716 t
ORIGIN      
        1 taaaaaattt gaaaaagcgt ttgattattt atattacgac ttagtgcaat aaaaaacgct
       61 ttttaacagt acatgtagtc gtctttataa tagataaact ctgtaatatt agttaaaaag
      121 ctataagttg cacttagtta agtgcaaaca tttcatattg tcgttagttt agaaaacgct
      181 tatattagca atgtaaggag agatgataat gcagcagcta acaaaaagag aaaggcagct
      241 tttgatttac ttggtggaaa tgtctgaacc ggtaacaatc aaaaagttgg ctgaaaaaga
      301 aaatgttagt gttcggacga taaaatacga tctagatgat ataagagagt ggctgaatga
      361 gcggaagcaa gtcctatata gcaaacgtag tcagggaatt tggatgaata ttagtagctc
      421 tgatcgaatc aaattaaaaa gcgaattgat ggatgttaat cgttttgaaa tatttgcaga
      481 tcaaagtctc cgaatcaaac gtttactaat tatgctttta gtaacaaaag aagctattac
      541 tggaatgaaa atggccaacg cattagatgt cagcaaagat acgatcatga atgatttaga
      601 tgtgctggaa aataattttc ttaaggaagg attgaccttg aatcgtcagg cgcgaaaggg
      661 tttttggatc ataggagaag aacgtctagt tcgattaacg attgaagaga ttcttcaaaa
      721 agagttcacg gattatgaca tttataaatt aatgtcattg ttattgaatg gcggtgagac
      781 tgaatacttt gaaatgtatt ctgctactgc tacacctatc caagaggtat ttaatcaagt
      841 aatcattcga atgcgacact tactggaatt tgaaaatcta gagaaactga attacgcgga
      901 attactaaat attctgattc gagtgacaat tgctaccgtt cgcttacgca aagaggctac
      961 gattggtcgt tatcaattgg ttgctgaaca agaattagac aaaaacgatc tttcttatcg
     1021 cgtcatgcag caggtctttg attattatca gttgccactg ttcgaggatg aatatcatta
     1081 tatctatagt gatacattcg acacgattca ccagcaagac gtcatgaaac tagttgagaa
     1141 tgtgatttat aaagtaagta aagcaatcaa ccattctttt tatcaagatc cacaattgtt
     1201 gacgaatctg tatgctcatt tatccatgcg attatcgaga aagaaaaaat ttgtcaatga
     1261 atataatcct tttaaagaag atatcaagca gaaatacccg gaattatttg atgcgatcaa
     1321 gcaagcagtt cgacaagaaa taagtgaaca aaatattctg atcaatgatt ctttcgtggc
     1381 atatattgcc ttacactttt tggtttccta cgaaagagaa gaagatctac gaagcgttag
     1441 agccgtctac atttgttcaa cagggttggg tgtgactagt ctgatcaagc aaaagatcaa
     1501 tgaagaatta aataatatca ctatcgtggc atttgcttca gtattgaatg cgcaagaaat
     1561 gattgctacc aaagatcctg atttggtgat tagtattttt ccaattaatg gcatcgatcg
     1621 tcctttcatc aaagtccatc cattacccac tgaacaggac ttgaaaaaaa ttcaacaaat
     1681 aataaaaaaa atacttgctt cttccaatca gcaggaaagg cataacttga atcttagaga
     1741 aacgtccatc aataaagata ctttagaaga ctttagcaag gaattgattt tgaaagctta
     1801 tacgatttat gaacgtttac ttgtcatttt ggaagcagaa ttgatccaag aatatcgaga
     1861 agcttttttg cttcatgtat tcttaatggt tcatcgtatc acatttgatc aacaatatat
     1921 ggcagaaggt cgtcaagtag accagattat tttagacaaa ggaacactgg taggaaaaat
     1981 cgaagcagtt tttgcagaaa ataatttagg aatcaatcag tcagaaatca gtgcattatt
     2041 ctcatatata aaagaaggag cgtcaagtta gtgaaaatgt cagacgaggc tttaaaaatt
     2101 atcgaggaaa gtcaaaatcg ggaaaagtta aaacaagtga ttgatctaat tgaagagcga
     2161 ttagtagaac atgctattgt accaaccgag ttgcagtgga ccattctaat taatcacttg
     2221 aatgagatgc tgaaacgttc tcaaagaaag gaaacgattc ccgtagtcga tcgggatttg
     2281 ttcaaagaag tatccaaaga agccataact atttctgatg aaatagttag aagtatcggt
     2341 aatttaaccg atgatgaaat atatgttttg tcgattcatt ttgaaacagc aaaaaataat
     2401 ta
//
LOCUS       DACEKH010000177         2330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEKH010000177 DACEKH010000000
VERSION     DACEKH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.186"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1356)
                     /locus_tag="IW662_002678"
     CDS             complement(join(<1..783,783..1356))
                     /locus_tag="IW662_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HAQ0867465.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NN"
     gene            1599..2054
                     /gene="tnpA"
                     /locus_tag="IW662_002679"
     CDS             1599..2054
                     /gene="tnpA"
                     /locus_tag="IW662_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0867466.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          694 a          442 c          446 g          748 t
ORIGIN      
        1 cattattgta gtaatagata tagctatcga tggctgattt taattcctcg aaacagttat
       61 aaactttccc gtgaaaaatc tcttgtttta ataagccaaa aaaattctcc ataagtgaat
      121 tatctaagca gttccctttc cgagacatgc tttgaaagat tttgtactcc ttcaatttgt
      181 ttctataagc tttcatttga tacgcccaac cttgatctga gtgaaacgta cgacgatagg
      241 gacaatcgtt agtggtctgg atagcttcct ccaaaccttc cattaccgct aatgcattgg
      301 gcttttcaga gatacgatag gataaaatct cactattgaa catatccata aaaggatcta
      361 gatacaattt cttttgacgg ataatgcctt tggcatccac ttcatggtat ttaaattcag
      421 ttgtatccgt cgtgattttc tgatggcaga tactggtgta gaaacgacga tggatccgat
      481 ttttggctac tgctccgact tgtcctcgat aagaattgta gcgccgtgat ttacgtgtgt
      541 atgtcgttac ttttattcct agcttcttga tcaggcgctg aactttcttt ttattgatag
      601 agaaaccgcg gtttcttagt tcattggtca tccgtaaaca gccataatcc ttgtgcttct
      661 cccgaacttt aagcatttcc gcttcaatct cttgatctgg atttttacgg tccaatcgtt
      721 tttgccagta cataaagatt gccttaggga actttaaagt ttctagaagc tcaactaatt
      781 taaacggtcc tcggaggctt gcgatgattc gtgcgattgc ttcattcgtc gttgttgggc
      841 ttcctgtttt cgcagcttcc tcaattcttt taaaaaggca ttttcaattt gaagggaacg
      901 gactcgtttc tccaactcct cgatgcgttc acgttcttta gacgttgctt tagttgtaga
      961 ttttttctta tttttgggtt catttttctt agacaagata ggtggacatc ctttcgtttt
     1021 tgagagtcca tcgatcccac cctcacggaa ttgacgcatc caactagcga tcaaattagg
     1081 gttgttcatg ttaagtgtgt tggcaacttc acgataggac agctcacttg ttagatataa
     1141 ctctatcgca tccagcttaa attgaacaga atattttttc ttttgtcgct ttcttactag
     1201 accctcttca ccaaattctt gataggcatt gatccagcgc ttcacttgtg atggagcctt
     1261 aatggaatat ttctttgcca gataatcact tccgccttta ccatccaagt aatcatgaac
     1321 gatctttaat ttaagttcaa aagtatactt agccataaaa aataccgacc tccaaaagtt
     1381 agattttttg gtctaacttt tgggggtcgg tacaaaactt aaaagtttta gaccctttta
     1441 ttttcttaat gacttcagtc agttttgtgc gtaaagcgtg cgaaacaaga aaaccgcgcg
     1501 tttctacgcg tggaagaagt atctttagcg ataaaaactc ctttcgatat actagtgagt
     1561 ggctaccaac cgctcatcag atcgaaagga gttttttcat gtcaaatgac gataaaagtt
     1621 tagcatatac tcgttggaat tgtaagtatc atattatttt tacaccaaaa tatcaacgaa
     1681 aagtaatcta tgggcaatta cgaaaagatc ttggaaaaat acttaggaag ttgtgcgaaa
     1741 tgaaagatgt ggaaatcata gaagcacatg caatgctaga tcatattcac atgcttgttc
     1801 gagtaccacc aaaaatatgt atatccagtt ttatggggta tctgaaaggt cgtagtgcgg
     1861 tactaatcca cgagcagcac gcaaatctca aatataaata cggaaacaaa tccttttggt
     1921 cgaagagata ctatgtgagt acagtcggat tgaatcaaaa gacaaatgaa aaatacatcc
     1981 gtgaacaaga agctgatgat cgcattcgat atattataag taaaagagag tacgtagaac
     2041 catttaaaaa ataaagagaa gtaagtgagt gaagatgagc agttggcggt cttttaaaaa
     2101 tcccctttta aggggcagcg agtgtcgacc ccttctaggg ggattaaaaa tacacccgtc
     2161 ctcacgggtg tatgggttac tgatagactt gattttaact ggtgtcagaa acttatgtcc
     2221 agaaagggtg atgaaatttt ggcaatttac ggctattcaa gaattagcac aagcaaccaa
     2281 gattacaaaa cacaaattca aacattagaa aatgctggcg cagaaataat
//
LOCUS       DACEKH010000178         2329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEKH010000178 DACEKH010000000
VERSION     DACEKH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.187"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            33..1844
                     /locus_tag="IW662_002680"
     CDS             33..1844
                     /locus_tag="IW662_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0867467.1"
                     /translation="MNENGGFEESKKYQATSEEEKAAVRELLEEAAPTDSLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVRETEATPEDKKR
                     IESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAENKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            1905..>2329
                     /locus_tag="IW662_002681"
     CDS             1905..>2329
                     /locus_tag="IW662_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867468.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYA"
BASE COUNT          906 a          371 c          505 g          547 t
ORIGIN      
        1 ttttgaaaga ctatcgaaaa gttgctttag aactgaatga aaacggggga tttgaggaat
       61 caaaaaaata ccaagccacg tcggaagagg aaaaagcagc tgttcgtgaa ttacttgaag
      121 aggctgcgcc aactgattct ttgaaggaga aagaaaacga tcatttctat cgagtagaat
      181 tcaacgaaaa tgatccagaa aaaggattga aaagttatgg tggggagatc gtaacattag
      241 aattgatcga tcaactcaaa acgttggata accaatacgc cgcagaagca ggttattata
      301 aattctattt tgaagaagtg aagaacggag aagtagtcga tcagatccga atggatctag
      361 gggatggcgt caacagtaat caacccatct atttagagtt agcaaaattg tgcgaacaag
      421 tacctgaaaa ggaacaaaaa caaacggtca aaaatgaacc tgtttcgaaa gaacaaatgc
      481 agtcgaaaga aacaaaaaca aaacaaaagg gctatcaaaa acgaacggca gaagagatta
      541 agcaagaagt caaatccctt tcgcaacagg cactggatgc ggtgaaaaag catacccatt
      601 ctcctgaaga cgtgaaagaa cttttgaatt ttatgtctcg ttttccagaa cgatccttta
      661 gaaatcaatt attgattgag gaacaatttc caggtgcaac ggcttgttta ggtcgtgctg
      721 cgttgaaaaa agaaggaatc tatatcaaaa aaggggaaca agggaacaag atctttgtca
      781 gaaaaacggt caaaggattt tatgagaaag gacgtggctt tgttcgagaa acagaagcta
      841 caccagaaga taaaaaacga atcgaaagtg gtcaaatcga agtgatcaaa aaaccttact
      901 acacgattga aaaagtgttt gatatcacac aaacacagtt aaaacctgaa gactatccga
      961 aaatctttcc gaaccgtgtc tttgattttc aattggataa aaaaggacag tcagaacttc
     1021 aagctgggat tcaagcggta gccaacaata ttggaataca aattcgagat atgacagaaa
     1081 gtgaagtcta tcaaagagaa ttaggacaag cacggggggc gtatgttcgt aacgaattta
     1141 ctggcgaaga agaaatcgtc atgaatactc gaaatactcc tacgcagcat ttagctacaa
     1201 gtattcatga attagcccat gcaagaatgc acaaattttc agaattagat accgcaacta
     1261 aagaattgca ggcagaaatg acttcttata ttgtgtgcaa acattttgga atggacactt
     1321 cggagaaagc tattccttat attgcagcat ggacgaagaa tggtcagacg ttggatgata
     1381 aggaagcggc ggaacgtggg aaaatcatga atgatgtcag ccgtgtcgca aatgaattca
     1441 ttcaaaccat ttctaatgaa atcaatcaat acagagaaag agaaccagag atccaaccag
     1501 cagaagtgaa gcaggagcat aaaaatactg tagaaacggc agagaataag aatattgtgc
     1561 ccgttggttc actttggatc gacaagaaag acgggaaagt aatggaagaa gatacgggtc
     1621 gaacgttaca tttgaaggat gcggcgttta ttgaagatcc gcagcataat caagtgattt
     1681 taaaaggaaa aagttatgga aaagagattc aaacggcgtt accttcagaa gaatttgcgc
     1741 taggaaatcc attccgattt tcacttgaaa cagtgtcaga agtggccaaa cgaaaaaatg
     1801 aaatctatta tgaacaacaa ttgtcacaaa aagaacgcgt ataaaacaag taaaaaagac
     1861 tatctgaaaa acggatagtc ttttttcttt tgggaggttt gaaaatgacc gaaaaaaaaa
     1921 tgagtcttat tgatcgatgt aaacagatcg atatagtgga ttttgctcga aacaatggaa
     1981 tggctgtagt caataaagga cgagactatc gcttagaaga ccatgattca tttgtttttg
     2041 atcgaagaaa acaacgtttt tattggaaca gtcaaaatat tagtggcgac attattgaat
     2101 tagctaaact ttttttcata gacaaagaga tccaagactc taagcaacaa tttaaagcag
     2161 ccacagattt tattctaaag aatgaagaca aaacagaacg agtagaaaat cttcattttg
     2221 agacagaaaa gtacaaagat caccccgttg attatcaacc actcacagaa aaaggaagga
     2281 attatctgaa ggaagaacga aaactaccag agtggctgat tgattatgc
//
LOCUS       DACEKH010000179         2258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEKH010000179 DACEKH010000000
VERSION     DACEKH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.189"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..914)
                     /locus_tag="IW662_002682"
     CDS             complement(<1..914)
                     /locus_tag="IW662_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ0867469.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IW662_002683"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IW662_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ0867470.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          761 a          390 c          427 g          680 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga accattaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttat
//
LOCUS       DACEKH010000180         2230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEKH010000180 DACEKH010000000
VERSION     DACEKH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.190"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(94..390)
                     /locus_tag="IW662_002684"
     CDS             complement(94..390)
                     /locus_tag="IW662_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ0867471.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            746..1102
                     /locus_tag="IW662_002685"
     CDS             746..1102
                     /locus_tag="IW662_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0867472.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1112..2011
                     /locus_tag="IW662_002686"
     CDS             1112..2011
                     /locus_tag="IW662_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0867473.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          728 a          380 c          424 g          698 t
ORIGIN      
        1 aaagcaaatc gtagttgtca caatctcttc taaacgatgt tccatcagct gaccttcggt
       61 attagctcaa agcactgatt tcacaacctc tatttaggca tgtgtctcag caagcggttg
      121 atagttgatg atctgcattt ttcgataaaa atcggcggga agcttactcc accgttttaa
      181 taattgatag aaataactct cttctgcttt gattttatac tcgcttaata tcaatccaaa
      241 actattcgtt ttttttgaag ctaatttttt ataaatatcc aatgctttgg ttttatcttc
      301 attgacaaag agtgttttta cttcaatata agaaccctcg atattttgtg tttcttcgat
      361 aaaatagatg ttttttatat attccttcat agtgattcct ccaaagaagt ctttttattt
      421 gataagttta ctctaccgaa gagttgttaa taatccgtga acaaaaagta attctttttc
      481 caatagttgt taaaggtttt ttatttttta tagtattttt tgttatttaa gaaaggaata
      541 aggttaaaca aataacaggt tgctttttgg gtctttttcc gttcaagaaa ctgaacgaag
      601 aaaagggaaa gttgtctctt ttgtgataag agaaatcata ataaacgaat gacttacttt
      661 ccaataaatt gagaacagtc agaaccagac aaattagttg catctgatga ataaagtgat
      721 tattctatga catgaggtga tacagatgaa attagaagtg acaaaagaag cacaagaagt
      781 attgaagaat aaattagaag cagacgatca acttttatta gatatcgaaa acggtgacgg
      841 tccatttgca gaaagccaag tcagttgcca attggacacg gcatttcgat tgatcattgt
      901 caaaaaagat acacaaacag atctaagtct ttattctgta gtggctgata caccagttgg
      961 accaatcaaa atcaaagaca gtgcaatact ttacatggat gatccatcca cacttgcttt
     1021 agaaccaact tataatagtt tacaattaaa aggaccaagc ggcttgagaa aaggcaactt
     1081 gcaagttgtt cgaaaagact agagggataa aatgggaaaa ttggcgatca tcagcgacct
     1141 acatgtggac atcaagaaat tcggcgaatc agagctgatg cagctttggc aagttttgca
     1201 aaagcacaag atcactcggc ttcatctagc cggagatacg gctaataaag tagaccgttg
     1261 cctagctgtt gtcgaatttt tcgtcaataa aggattatct acgacatttc atttcggcaa
     1321 tcacgagttg gcagatgtgc ccaatgaaca gatgatggac cattttccag acccacattt
     1381 tttaaatgaa cggtatattg ctttaaatga gcagaccgtt cttttaggga tgaatggctg
     1441 gtacgactat cagttttccg aattgaaaga ttacgaggca atcgtacgaa tgaagcgttt
     1501 gtattggtat gatcggttca tccagcgtgg gaaaacagat ctcgaggtca atgcagaaat
     1561 gcttgcagct gcaaaaaatt tgctagacca gctggaagca aaaaaacttg aggtgatttt
     1621 atccactcat ttcgttccta aacgggcatt tatcatgtat caatctgctc cttatgagcg
     1681 ctggaacaaa ttaaatgcat ttttaggatc agaatcattc ggagaattac ttgaccgtta
     1741 ttcaaatatc aaacaagtag tttttggcca tacgcatcgt cgctttgaag ataaatccat
     1801 cggtcacacg atttacagct gcagaccttt tggttattat tatgaatggc agctgacccg
     1861 agattttgtc ttgaaagaac agctgttaga tagctatgaa cccatgaaat tacgtgtcct
     1921 cttgcgtgcc catcatccgg cctttcaagc ctatcaaaca caacatttgc aagaggaatt
     1981 tgaacaggcg atgacaatcg ttccgtattg agacagcatt agtaatgtgt aaaagaaaat
     2041 accttgtaaa acataaggat aagtaagagc agtagatcca tttatcgtgg atatgctgct
     2101 ttttttgtgg aaaataatca gcgaatgttt atttgatcgg atgatttgta actcgcagag
     2161 tactagatgc aagcgtttat tctttttttg aacgataatc acaaaaaaaa atataatata
     2221 cattttttat
//
LOCUS       DACEKH010000181         2228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEKH010000181 DACEKH010000000
VERSION     DACEKH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.191"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            8..550
                     /gene="sat4"
                     /locus_tag="IW662_002687"
     CDS             8..550
                     /gene="sat4"
                     /locus_tag="IW662_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ0867474.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            643..1437
                     /locus_tag="IW662_002688"
                     /note="aph(3')-IIIa"
     CDS             643..1437
                     /locus_tag="IW662_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0867475.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(1545..1733)
                     /locus_tag="IW662_002689"
                     /pseudo
     CDS             complement(1545..1733)
                     /locus_tag="IW662_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1713..>2228
                     /locus_tag="IW662_002690"
     CDS             1713..>2228
                     /locus_tag="IW662_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0867476.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIE"
BASE COUNT          753 a          362 c          527 g          586 t
ORIGIN      
        1 gggaacagtg attacagaaa tgaaagcagg gcacctgaaa gatatcgata aacccagcga
       61 accatttgag gtgataggta agattatacc gaggtatgaa aacgagaatt ggacctttac
      121 agaattactc tatgaagcgc catatttaaa aagctaccaa gacgaagagg atgaagagga
      181 tgaggaggca gattgccttg aatatattga caatactgat aagataatat atctttacta
      241 ccaagacgat aaatgcgtcg gaaaagttaa actgcgaaaa aattggaacc ggtacgctta
      301 tatagaagat atcgccgtat gtaaggattt cagggggcaa ggcataggca gcgcgcttat
      361 caatatatct atagaatggg caaagcataa aaacttgcat ggactaatgc ttgaaaccca
      421 ggacaataac cttatagctt gtaaattcta tcataattgt ggtttcaaaa tcggctccgt
      481 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
      541 aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg
      601 gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata
      661 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
      721 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
      781 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
      841 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
      901 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
      961 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
     1021 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
     1081 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa
     1141 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
     1201 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
     1261 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
     1321 tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
     1381 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac
     1441 ctagatttag atgtctaaaa agctttaact acaagctttt tagacatcta atcttttctg
     1501 aagtacatcc gcaactgtcc atactctgat gttttatatc ttttctaaaa gttcgctaga
     1561 tagagttcta tattagagat gtaaagagtt ttggtgaaga ggtttgtgag tatggctttt
     1621 ataaccaggg tgcaatgaga agcactaaca gcagctagct taagaacgca taggatatac
     1681 tttttatgga agtcacagag gaggaataaa atttgaacag gattaacaga gtaacctcta
     1741 ttcttattca gctgcaatca aaaaaaataa ttcctgccaa agaaattgca cagcgattta
     1801 atataagctt aaggacagtt tacagagata tccggacact tgaagaagcc ggaataccaa
     1861 ttggatctga ggccggaaaa ggatattttc ttgtagaggg cttcctactt ccgccggtaa
     1921 tgtttacagc ggcggaagtg ggtgcattga ttacagcagg gaaattttta aattgccatg
     1981 gagatgaatc atttataaag gattttgatt cagctatgta taaaatcaaa tctattttga
     2041 agcatggtga aaaaaactat gcacaggagt tggagaacag tattaacgtg tacagcacat
     2101 ctggacagaa aaatacattg gcggataacg ttattgcagc aattcagact gcaatctgca
     2161 ataaaagggt aatatcaatt caatatcctg catcaggagg gcaagaaccg gaaagcagaa
     2221 tgatagag
//
LOCUS       DACEKH010000182         2212 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEKH010000182 DACEKH010000000
VERSION     DACEKH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.192"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            137..1177
                     /locus_tag="IW662_002691"
     CDS             137..1177
                     /locus_tag="IW662_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0867477.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2001..>2212
                     /locus_tag="IW662_002692"
     CDS             2001..>2212
                     /locus_tag="IW662_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0867478.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIP"
BASE COUNT          849 a          310 c          370 g          683 t
ORIGIN      
        1 ttttatttct gattttaggt agtatttaat taaaaaacag ttttaatgcg tttgcatttt
       61 ttatttatct ttgctatttt cgaaaagagt aggtaatttt acataaataa aacaacgtga
      121 aagtagagga tctacaatga ctgattttaa attttttaag gcggatagag tttacaacga
      181 attattttat caatttccaa aagtctttat tgtttctgac gaatacaaaa aaatgaaaga
      241 ttcaactaag attgcctata tgcttttaaa agcaagatta gagatcgcaa tcagcaaacg
      301 gcaaatcgat gaagaaggta atgtttattt tacttatacg acaaatgaac tatgtagagt
      361 attaaactgc caaaaacaaa aagcgatagc aatcaaaaaa gagttggaat cctttggttt
      421 attattacaa aagcagatgg gatttaacaa acagttaggg aaaaataatc ctaatagact
      481 atatctagca gaattaaaag tctcagaaaa tgatatctac ttactcgaaa aatttgatag
      541 agagaatagg gaaaacgttg ataaatcaga gggtatgaaa atcataccca ccctcgacga
      601 aaaatcagac gctgaatccc ttggggctca agagggtatg aaaatcatac cgtgccaaaa
      661 cgttgataaa tcagagggta tgaaaatcat accagaactt aataataata tattagacac
      721 taatagacac aatatagaca ctgaaaaaga ccgcctacaa gatcaattgt tgttagacaa
      781 ttttgagaca attatgacaa acgacagcat tgctacgttt gtccctgaac gagtattaaa
      841 tttgataaaa acattttctt caagttacag tgaagctcaa aaaaccgtcc agactattca
      901 taatgcaaag aaaaaagctg aaatagaaag tggtatttcg atagtttttg aagaactcga
      961 tagttattat gtcaatgcag aacaagaatt atacacgaca ctgttaaaag cctatcaaaa
     1021 attaaaaacc gaaaaagtcg aaaatatcca gaacctgatt tttgtctatg taaaaaattg
     1081 gtttatcgaa aaaccaatag ctgctaaagt atcaagtgaa aaacgtttga attatgaaag
     1141 ctccccaagc actattacga aagactggtt agagtgaaac tttactgaca aagtgattgt
     1201 tgcttcatgg agtaacatta aaattaaaaa ttttttatta ttctaattat attacgaata
     1261 ttacaaaata atagctattt taatagaatt tataagttaa aatattatga taaaaatcac
     1321 aaacgtagcc ttttacgtca taagtgttaa aagaaataat taatttcaaa tatagaaaag
     1381 ggaacgtatg tttgcatact cctttttttt gagttataat aatacgcgaa gagagataat
     1441 ttcattattt aaacaagcta aaaatcttga tattacttct tttcaggcaa tacgaatatt
     1501 attcgcttac attttttgta taaatttaat tgtctgctat gcttgtgatg aattgatttt
     1561 cgttctgcac ttttggatga taaatttgga caattgaaaa tacaaaaaaa agaaaggcgg
     1621 tatgaactcg gaaggctggt aaccttcgtc agcgttcata cacggcatta agatttggcc
     1681 cttaatgcgt taacctttca gtgttacggt attacccgca atggatttag tataacagat
     1741 attcaaaagt gataccactt tttttggctg tgttaaggtt aaaaatacgt gatattacga
     1801 tgaatctatg cctaagcata atgttataaa aatgtccgtg gggctctttg ccgtatgacg
     1861 aacgctgaaa ttagagggaa cgcatgtttg aatgcgttcc ctcttttttg tttgtcatga
     1921 acgaaaatca attctctatt tttgaaaaat aacaagaaaa acttgttaaa aatatttaaa
     1981 gaaattgggt ggtttatttt atgtcatata caggaatttt gagtctcaaa gatatttgtc
     2041 actacggtaa acggtgtacc gctacagaga aaatcacgaa gaaactatca actggccaaa
     2101 gtaaaaccgt tgtgcagtgt aagaaatata taatccagaa agacaaagtc tcagaggaaa
     2161 tgatttatta cattgggaaa caaaagcaaa taattttaaa agatcctatc cc
//
LOCUS       DACEKH010000183         2183 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEKH010000183 DACEKH010000000
VERSION     DACEKH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.193"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IW662_002693"
     CDS             complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IW662_002693"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ0867479.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          581 a          475 c          397 g          730 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac
      361 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatg tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat
      481 catgagggtg tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac
      541 gtaattgctg tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca
      601 tttggaaaat aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg
      661 gtactaattt atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt
      721 ccatcgcgcc taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt
      781 cgaattcacc tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc
      841 cgccggcagc taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa
      901 tcgctttgcc atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta
      961 cacctacacc ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta
     1021 cgacatctac acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg
     1081 caatcaatgt tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg
     1141 cactatgtcc atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat
     1201 tcgcacgctc aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat
     1261 gttcatcttt tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca
     1321 atccgcttaa acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca
     1381 aaatcttttc tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg
     1441 tcatcacatc agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa
     1501 ttatccctac caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat
     1561 atttgctcat caattcttct gcgtctgcta caagatgctg tggcgttaag aagaacggat
     1621 caatgattac gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga
     1681 tcgtcatgtt tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct
     1741 tgctgtcggt aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt
     1801 tagctaattg tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca
     1861 ataagacatc atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt
     1921 ttagtaccac tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc
     1981 aatgatctac ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat
     2041 ctgtgtatat taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca
     2101 atcaaaaaca gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt
     2161 ttgaacaccg tttagtggag agg
//
LOCUS       DACEKH010000184         2176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEKH010000184 DACEKH010000000
VERSION     DACEKH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.194"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..338
                     /locus_tag="IW662_002694"
     CDS             <1..338
                     /locus_tag="IW662_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0867480.1"
                     /translation="ADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPII
                     LADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKK
                     VNKMNLEVE"
     gene            338..1714
                     /locus_tag="IW662_002695"
     CDS             338..1714
                     /locus_tag="IW662_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0867481.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            1844..>2176
                     /locus_tag="IW662_002696"
     CDS             1844..>2176
                     /locus_tag="IW662_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0867482.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT          845 a          270 c          400 g          661 t
ORIGIN      
        1 cagcagatga aagtatcttg tttgaactag gtttagataa aaaacaaata aaaagaaatg
       61 ttatgaaatt atctggtgga cagcaacaaa gagtagctat tgctagagca ttggtatcag
      121 atgctccaat aatactagct gatgagccta ctggtaatct agacagtgtt actgctggag
      181 aaataattaa tatattaaag acattagcta aagatagaaa taaatgtgta atagttgtaa
      241 cacatagtaa ggaagtagca gattctgcgg atatcatttt agaactaagt ggtaaaaagt
      301 tgaaaaaagt aaataaaatg aatttggagg ttgaataatg ataaaaaatg catttgctta
      361 tgtaactaga aaaagtttaa aatcattaat tatcatatta gttattttat ctatgtcaac
      421 tttaagtctc attagtttat ctattaaaga tgctacggac agagcttcaa aagaaacatt
      481 tgctaatata acaaatagtt tttctatgga gataaataga caagtaaatc caggaacacc
      541 tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag atatctcaaa cagatagtat
      601 agactcctat gtaaaaagaa taaacagtgt tgcagattta gttgattatg atattattga
      661 aactcaagag actcttgcga atcaatcacc tgaaagagca aagaatttta aaagaacagt
      721 tatgttaact ggggttaacg actcaacaaa agaaacaaaa tttgtatcag aagcatataa
      781 attagtagaa ggaaagcatt tagaaaatga agataaaaat aaaatcttaa tgcataaaga
      841 tttagctgaa aaaaataacc ttaaagttgg agataagata aaaataaaat ctaatttatt
      901 tgatgctgat aatgagaaag gtgcagatga aacagtagaa gtagaaatta aaggtctatt
      961 cgatgggcat aatagcggtg tagtaagtgc agcacaagaa ctatacgaaa atacattaat
     1021 cactgatatt catacagctg ctaaagttta tggtaatacc gaagatacag ctgtatacca
     1081 agatgcaaca ttctttgtaa aaggtgataa gaatcttgat agtgtaataa aagatcttgg
     1141 gaaattagat ataaattgga gagaatataa tttgattaaa agttcatcaa attaccctgc
     1201 attacaacaa tctatatcag gtatttactc aatttcaaat aaattatttt ttggctcatt
     1261 aatatttgca ggagttgtag tttcattatt attattctta tggatgaatg ctagaaagaa
     1321 agaaatagca gtattactat cattaggtat atcaaaatta gaaatttttg gtcaattcct
     1381 aattgagatg gtatttatat caatccccgc attcgttggt tcctatttct tagctcaata
     1441 tacagctgac aagcttggga ataatatatt gaataaggtt actggtgata tagctaaaca
     1501 aatagctaaa caatctgcat ctagccaatt aggtggtggg gcagaagctg aaggatttaa
     1561 caagacatta tcaagtttag atatcaatgt attacctaaa ttcatgattt atgtagttat
     1621 atttatgagt ttagtacttc ttgtatcatt gattatttct tcattcaata tattaagaag
     1681 gaatccaaaa gaattattaa ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa
     1741 aatgagagtg taatataaac cgtgtaagta accatcaatc ctaggcgggt ggttgcttca
     1801 cggtttttat ttttctaaaa taagatcaag aggtgattac acgatggcta gaaagaaaag
     1861 aaatcctgat gctgagaagt tagctgaatc cattttgaat acttatcaac ccgaatctgt
     1921 agaggatatg caagatgttt taaaagatgt gtttggtccc ttatttgaaa aaatgcttca
     1981 aggagaattg aataaccatt tagggtatga cgcccattct aaagagccaa agaaacatga
     2041 caatcgacga aacggttatg gcaataagac gcttaaaacg agctttggta aagtaaacat
     2101 tgatgttccc agagaccggg aggcttcttt tgaacctgag ttgattccta atagaaaacg
     2161 agatgtctcc gacatc
//
LOCUS       DACEKH010000185         2165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEKH010000185 DACEKH010000000
VERSION     DACEKH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.195"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            622..801
                     /locus_tag="IW662_002697"
     CDS             622..801
                     /locus_tag="IW662_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867483.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            860..1216
                     /locus_tag="IW662_002698"
     CDS             860..1216
                     /locus_tag="IW662_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867484.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT          772 a          299 c          409 g          685 t
ORIGIN      
        1 gtcaaaccaa gcactattaa accttctgaa taccaagctt tagaactaac gtatgcgaaa
       61 tttatcgaaa cgaaaaaagc caagactgtt tcaagtgaat ggttagatta ttttgaaaat
      121 atcaataagt atggcgcata ttacaccatg aaaaaggaag acaacgaaaa tgaataaggt
      181 gatttatcaa ctaacaacgg attccatatc aacgatttga aagaaaaaaa ggtcaataat
      241 catttatacc gaaaagtgag aatgtgtgaa acaaggacaa aaaacgccta gtaactaagg
      301 aaatggttct tatttcgcac caatcgctcc aaccgccccc gccgcttagg tggtgcgaaa
      361 taaacgggca aagcccgata acttaagaga aagaaggtga tccatttgaa caagattatt
      421 cccttattgt tagtactgtt tgttggcgaa atacttatgc gagatagtgc agctgtacag
      481 ttaaacctcg ctgtattttt ggtaatactg gtgttttgtt tgatcttttg gcggtttgta
      541 ttagtcctcg tgagtctact attcaagatg attgcagcca tatttttcat agggttattt
      601 cgatgaaagg agtgagtacc aatgccaatt gcaataggaa ataaacggtt acccgtgaca
      661 ttagatgaaa agagacaaaa agaattgcag caactaaagc agaagtatgg caaaagtgaa
      721 tctaagatta tgtgtattgc gttagatcta ttgatcgcgc aagaaaaagc aggatttgat
      781 gtaccagcgc taaaaaagtg acgtcacttt ttaatttgat ctatataagt gatatcactt
      841 ttaccaagga gggaattaaa tgttaaaaac taaaaagaat tcgtacaaat taaagcttag
      901 tatactatct attatttgtc ttttaggcat gataattagt ggtcatttgt atagagtggc
      961 atatacgcag tttattgagt acaaggttca ctctttagga ttagctgggg atcgatacgt
     1021 tgaatatgtc cattattcat taaaatgttc ttcatatatt cttctttggc ttattttttc
     1081 attgtttgaa ttgctaaatt taatagcaat atcttcatat cacttagata agctaaatac
     1141 caaatctagc cgttataacg aagtgtttaa ggaaaatgtg aagttagaaa gtgacttaaa
     1201 caagctaaaa aaataaaaag aagaaaacat aagttaacta aaattatttt attaagaatg
     1261 atggaatata aggataaggt taccaacata acgatcatta cttctctgat atgctatgga
     1321 cttctatcaa tcagtatttt gtctatagat tatggagtaa ctatactgca agccttagtt
     1381 ttggctatgc ctatgtatct atctttgttt atcatgcatg aaatatataa gctatttgtt
     1441 ttatcaaaaa atgtagaaat acgggtaaat attttggtga atagttatca aaaagaactc
     1501 aaagaaagaa gacggtggcg gtatttattg aaaggatgaa aaagtaggat ttgacatact
     1561 ggtattcaga aagtgccgtc acttttaaaa caatggaaaa ggtggagata agatgaagaa
     1621 aaaacaaact aaactatcat tagcagatat ttaacacagt taaccgcaac tgaagatggt
     1681 gttgttgaat ttgaacaatc tgaaattgcg gtagtagatg atcgttacct taaaatgcct
     1741 tacttctttg accaagcaaa ggtcatttgc ctttgtcact acgatggggt acgtgatttt
     1801 aggcgttaaa aagtagtgat tatatcaact ttacgagtaa atataaattt agacatgaaa
     1861 ctgaattatc caatggaaaa tcatggttta taatctagaa aaattatagt aatgattgga
     1921 ttatatatat tctttcgtat tttcgtacaa ctgtagcttg tcaaaattga gaaatttaac
     1981 cgattaggta ctttacatcg ttatttgggt aatttctaaa acatgaagct taccaacgaa
     2041 taaactgaga ctatcttatt aattgatcaa tataagaaaa tatctttgag gagatgatgt
     2101 actatgtttt cgtctatcca cgattggtta ggtggtcttt tcggcggtgg aggtaaaggt
     2161 tttta
//
LOCUS       DACEKH010000186         2147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEKH010000186 DACEKH010000000
VERSION     DACEKH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.196"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            915..1388
                     /locus_tag="IW662_002699"
     CDS             915..1388
                     /locus_tag="IW662_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0867485.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1397..1624
                     /locus_tag="IW662_002700"
     CDS             1397..1624
                     /locus_tag="IW662_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0867486.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          690 a          342 c          318 g          797 t
ORIGIN      
        1 tagttacttt aatgcgttct tactttattt ttatattatc atttttctta ttcacttctt
       61 tggtttatac tattcttata aataaaaacg aggtatgtcc tatgatgaaa agattgtact
      121 attcattgat cataacgatt ggctatttga ttgtatcaaa tctaggaaat atggtatttg
      181 gtatcagtaa agaattcagt tggactacta cattatggga atctctattc tttttcatct
      241 tcgtattttt gttacaaaat tatcgaaaaa aataaacatg tgccatctat ctgttggcac
      301 atgttttagg atttatggta ctttttattc ggcaatccat tttatttttc tacttcttct
      361 taatacctct aacacttttg atttataaac taatccaatg catctacttg cgcttattaa
      421 gctatttcag ctagatagta ccaccccatg cgtcaatgct tgattttcct tttgtataac
      481 attgtataag caataaagga caaataagtg gtgattacac caaaccattg aataagaaaa
      541 attctgatac gtaaataaac atgaacttga aaaaattaat gaatcggtta gctactacgt
      601 agttgtcttt tttctcatac agttctgtta acttacactt ctcttcgcta gataataaaa
      661 aatcctccaa ctgtattgtc caatataatc ccaggcatct aaactagcat tatagaagag
      721 acacacatag aatctaaaat aactggtttt aaatgtttct tacaatcaaa aaaagctgtt
      781 aaatgcactc cacaccttca tactctatca ttttttataa acgatatatt tttattgttt
      841 ttcgataaaa aatagttgtt ttacaataat tattctgcta tattttagct atcaaagata
      901 aaagagggat aaaaatgaaa tttaaaacat tgctattaaa atttgtcgtt ttaggtataa
      961 gtgccgtatt tattttgttc ggcgtacttt tttatactca gatcgctaca agcgaaaaag
     1021 gattcaattt tgattggcag accacattat ttgtttctgt catttacttt gcccttctcc
     1081 tagggatcgt tattgcttat ttcttattga aactattgaa aaaaatcgaa gataaccaag
     1141 ctttttccac tcaaagctta aacatactaa aaaaaatcga acgaaccatt ttgtttattg
     1201 gtttcgcctt ttttggctta cttcctaaat ttatgaaagt agcggattta gacgatgctc
     1261 caggtttgat gttggtagga atcgctgtcg tattcattcc ttttgcagta tacacattaa
     1321 caagcgtgct ggaaaaactg cttgaacatg cgattttact taagacagat catgatttga
     1381 cagtgtgagg tgcgacatgg caattatcgt taatttagat gtagtattag ctcaaaaaaa
     1441 aatgagtgtg actgaattga gtgagaaagt gggcattacg atggcaaaca tctctattct
     1501 aaaaaatgga aaagcaaaag ccatccgttt ttccactctc gaaaaaatct gccaagtttt
     1561 ggagtgtcaa ccaggtgatt tgttgaccta taaaaatgac tcgaatacta taccagaaga
     1621 ataaaataaa gtagaacaaa cagtaaataa aaaataatct gaatgataga tgtgtgagac
     1681 ttttagaagt tatttacgtc tattattcag attttttatt tcgattgaat accgttttat
     1741 ctataaataa gatatgtact gactcataca aagggggcct tttcttctcc atgtactatc
     1801 acttttcctg atacaactgg tttcctgtta cgttgtagtt ggatctgctt ttagtataat
     1861 actttattca ttccaccaga ctgatagcag tttgagcagt ctaaataatc atgtagtatt
     1921 acaggccatt ctgtttcata ccttgaagtg cttaaaactt cagctaatgg cccattgtgt
     1981 taactcttac tccattcttt tttagtaagc tcccatatat actgtaaagc cttctatata
     2041 ttatgtcatg ttgcttttta acatttacta tgtagttttg ataaaatgat ttgaataaca
     2101 gtgatggaca attctttttt atttttaaca ttattttgat aataatg
//
LOCUS       DACEKH010000187         2079 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEKH010000187 DACEKH010000000
VERSION     DACEKH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.197"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..424)
                     /locus_tag="IW662_002701"
     CDS             complement(<1..424)
                     /locus_tag="IW662_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0867487.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="IW662_002702"
     CDS             complement(533..1564)
                     /locus_tag="IW662_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0867488.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          644 a          336 c          359 g          740 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcca tttattcaat aatggtttgt ctaacgatat tgttgggata
     1921 attcttaaag aagtattaga aatagttggt acttcattac tttacatgaa gatttattct
     1981 atggtaggag caattatatt gagaattaat ccttttttat ataagttgtt tgcttattga
     2041 tggattggat aaattaaacc atcacaacga gtggaaatt
//
LOCUS       DACEKH010000188         2069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEKH010000188 DACEKH010000000
VERSION     DACEKH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.198"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            88..702
                     /locus_tag="IW662_002703"
     CDS             88..702
                     /locus_tag="IW662_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0867489.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            852..>2069
                     /locus_tag="IW662_002704"
     CDS             852..>2069
                     /locus_tag="IW662_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0867490.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNA"
BASE COUNT          688 a          402 c          400 g          579 t
ORIGIN      
        1 tagacactgg tataacattg aaaaatatgg cttcctcgtg tatgattagg gtgtatttta
       61 aatagttgca gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc
      121 gatcaaaatg aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc
      181 atcgagaaac aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat
      241 tacgtccgag atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat
      301 gacgagatca tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact
      361 agtttaccga tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat
      421 ctgatcatcc aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc
      481 caggcacaag gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta
      541 tacagtgcag acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat
      601 cttaaaaatg gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc
      661 gtgtatagaa taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta
      721 ttatcggctc tatacttttt gatgttggtg gatgacacca gcatcttttt tattttccaa
      781 aacatataga aaagacacct tgaaatcctt tagaattaat ttggcgaacc aacccaaaag
      841 gaggaatcaa gatgtcttac actcacctta tcaaagaaac attagatatt ctagacttat
      901 cagttacttt taacgaaaat tgtctcacaa aagaaaaata caaagggcaa atctgtcata
      961 tttatcgtgg aaacttgatt tatacagccc aagaatgcat ccactgtaaa caccagattg
     1021 cttcagatat tgtgcgttgg ggtacaacaa ccgtccggtt gttaatgaac gatgtttctg
     1081 aataccgtac ctacctcgaa ttgaaaaagc aacgcttcaa atgcaaagct tgtcagcgga
     1141 cctttgtcgc agatacttct gttgcgaaaa aacattgttt catcagtgag aaagtccgct
     1201 ggtctgtcgt gactcgtctg aaaaaaaata cctcaatgac agagatcgcc gctcagaaaa
     1261 atctatcggt ctcttctgtt taccgtatta tgaagcgatt ttatcgtccg ttgaatcctt
     1321 ttcggacacc gttacctaag gtactttgtt ttgatgaatt caaatcagtt cggggtgttt
     1381 caggtgctat gagtttcatc atgatggatg gacaaacaca gcgcttattg gacattgtcg
     1441 agaaccgaca actgcctttt ttaaaacgtt attttagtca tttttcacga gaaattcgtg
     1501 aagcggtcga atggatcgtg attgatatgt acgctcctta tgtatcgctc gttaaaaagc
     1561 tgtttccaaa agcacagctg attattgacc ggtttcacat cgtccaacat attggtcgta
     1621 cctttcgcaa tcatcgaatc aaagaaacga accaactatt aaaaagcaaa gaacaaaaac
     1681 actatcaatt aggtaagcaa ttaaaaagat actggaaact tcttcaaaaa gatgaacgca
     1741 agcttgatta tactcgacgc ctttggcgac ctggcttcaa agctcattta acagagaccg
     1801 atattgtgga tcgcttactg aaagggagcc ctgctttgcg tgtaggctat cagttatatc
     1861 aagactttct ttacgctgta aaggaacgag actacgtatc gtttgaagaa ctacttacca
     1921 ataatatcat gcttcccgaa ggctatcaga cgaccttgag aacgttccag aaatttctgc
     1981 cacaaatcaa aaatgcttta caacaaagtt attcaaatgg cccacttgaa tgtctgaaca
     2041 accatatcaa agtgttaaaa cgaaatgca
//
LOCUS       DACEKH010000189         2034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEKH010000189 DACEKH010000000
VERSION     DACEKH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.199"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW662_002705"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW662_002705"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ0867491.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IW662_002706"
     CDS             complement(1431..1823)
                     /locus_tag="IW662_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0867492.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACEKH010000190         1990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEKH010000190 DACEKH010000000
VERSION     DACEKH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.200"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            612..1424
                     /locus_tag="IW662_002707"
     CDS             612..1424
                     /locus_tag="IW662_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867493.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          688 a          274 c          318 g          710 t
ORIGIN      
        1 atggcctttt tacttttaca caattataag gactttatcg aaaaaaagca gttatttgtt
       61 aaaatcattg atattattaa taataagtat tttttgaaag gggaaatata aaaggttttt
      121 gaaaataaat aaaaaatagt attacttgtt ctattgctag ttattgctag tctttattct
      181 tttttgtttt ttagggatgt acaaaagatc tcaaccacag gagacattgc ttttcacttt
      241 tctagaataa aatgcttgtc ctcagtattt tctggtccaa taaattttac cacatttaat
      301 agctatggcg gaggcgtgaa tttttttatc catatctaac atctttccag ccgtaatatt
      361 ttactggaca acaaacaatt tgattctaag ttatatactt actatacgtt gggatactga
      421 atatctgtac aattctagtg agctatcaat atggacaaaa gaatagatgc agccttctta
      481 ttcagctgtt tgtatagctt ttatggattg atatatatta gcgtactgca atttcagaat
      541 caattgcgtt gacgattgta ccagttgtct tgtattacac atatactctt ctttatgaaa
      601 gaaaaaactg cgtgattctt ttagctgtca gtatgagttt gctcatctat acccatattc
      661 tctccacctt tatgagtgtg attgttattg gcttcttgtt tatcggctct cttttctcta
      721 agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa tatgataaaa gctgttggtt
      781 ggaccattct tttaacagct tacgtttggt atccgatgtt acaacaagtc ctacatcaaa
      841 aaattaatcg tccttttcgc acaaaacttc aagaaagggc attaaacatt tctgatagtt
      901 taatcggtgc agtaaataat gatttagtta catttacaat gggaacaatt ggtgtggttt
      961 catttattct tccacttttc tttaaaaaaa aatttgccaa taaagaaaag attattaatt
     1021 tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt tccatgggaa tttttacaaa
     1081 aaacacctat tcaaatcatt cagtttcctt ggagaatctt gggttttcaa gtgctttttg
     1141 gttcattgat cttaattata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
     1201 gtagccttgt aggaatagtc cttcttattt ttacagtgac tgtagcaaca gaagcgaatt
     1261 attcgcagaa aattcaatcg tataaaggtc gtttaattat gaccaaaaaa gatgttacac
     1321 tttatacaac ctcgagaact ggaggtcttt atgactatgc accattagat gcattgaaat
     1381 ataaagatca tttaaagaaa catgaagtaa aagtctatga ttgatgggag aatgcatctc
     1441 ataaagcaca taattctaaa ataatctata cagtaggaga tgcgaatgag caaaatatca
     1501 cgttacctgt atttaattac tgggaacagt tatgaaagta aatgggaaaa agagcactat
     1561 agaaaatgac aatggtttag tgtctattaa aggtacggga aaaagaacta tagttgaggt
     1621 ttcatctacg tatccaacta cgatgattct atctttatta attagttcaa taacttttat
     1681 attcttattt acacgttatt taataagatg ctgcggttaa atgaaaatag attagataga
     1741 tattataaat atgttgattc tattttcata gcaatggaga aaatacatta gcaatatata
     1801 aaagaaggta tcaagagtca cggtgaattt attccgaaaa gtgaaaagaa caaatataaa
     1861 atttaataaa aatatagaac taaataggaa aacgtagacg taattttcta cgttttcctt
     1921 ttcatatata ttaaatatta cggaaattta tataaactaa tttagtgttt tgtttgtaat
     1981 tcctaatttt
//
LOCUS       DACEKH010000191         1965 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEKH010000191 DACEKH010000000
VERSION     DACEKH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.201"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            74..592
                     /locus_tag="IW662_002708"
     CDS             74..592
                     /locus_tag="IW662_002708"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0867494.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            720..1403
                     /locus_tag="IW662_002709"
     CDS             720..1403
                     /locus_tag="IW662_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ0867495.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1712..1785
                     /locus_tag="IW662_002710"
     tRNA            1712..1785
                     /locus_tag="IW662_002710"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1746..1748,aa:Arg,seq:tct)
BASE COUNT          671 a          305 c          395 g          594 t
ORIGIN      
        1 agctataatt tgaaaaaaat ttatcgttat tatatgatta agaaggattg aataaagaaa
       61 gaagttgaaa acgatacctg attacaaaaa aataactttt ataagttcta taacgttgat
      121 tatattttta attcttattc ttctcgtcgt cttggttccc cgaatttttg gtttgactcc
      181 ttatattatt gaggacaaag aaatgagtcc atattatgca caaggaaatc tgatatatgt
      241 acgaccgaaa aatacaaatg agattttagt cggagatatt attacatatt atgaaaattc
      301 aggaaaaaaa atctcgacgc gtcgcgtaat agccgttgaa aacaaccacg aagattttta
      361 tacaaaagct gatacaaaga cttatattga gcctggagta gtaaaaaaga gaaatattat
      421 cggtagtccc atctttcaga ttccatatat tggactagtc ctttcaaaaa cggcatttgc
      481 atggattcag gggttattct ttacgattgc tttttgccta acaggaatca cagcatggaa
      541 tacctttgta gagttgaaaa agaagcggag acgaacacag aaatttttgt aagaatgtta
      601 aaaacataaa tgtattccag agatccaacg actcttctga acatgaaaca cgagaaaaca
      661 ctcatgcagc ttgtagattt agtcagttgt tcgtataatg tgtgaagagg aggagtagta
      721 tgcgcaatga aatgatgcat cgtcctgtag tcaaagaaga actattggat tttatgcgta
      781 cccagcaaaa aaaattaccg ggtgagctag gcaaactgga agaagaagct catgtagcag
      841 aagtaccagt tattccccat gaaacagttg tatttttgaa atttttactt ggacagcttc
      901 agcctgaacg aatcttggag atcggagcag ctatcggttt ttcatcaagt gtcatggcca
      961 cgacaatcgg agaaaacgct catgttacga caattgatcg tttcgatgtc atgatcgaga
     1021 aagctaaaaa aaactatgaa cgtctaggac tgacagataa agtgactttg cttgaaggtc
     1081 aagcagcaga tatcttgccg gagttgtctg gtccgtatga ctttattttt atggatagtg
     1141 caaaatcaaa atatattgaa tttttacctg aatgcttacg tttgttaaga aaaggtggcg
     1201 ttttgatggt agatgacatc ttccaaggag gaacgatctt gctcccagat gaagagattc
     1261 cccgtggcaa acgagctatc catcgtaaat taaatgaatt tcttcgtgtt gtgatggatc
     1321 atcccgattt gacatcgaca atcttgccat taggtgatgg tgtgatctta atgacaaagg
     1381 aatcagaaac aatcgagttg tgatacatga ttatggcagc ctgtagataa tcgattgaat
     1441 cgagggctca attcgaactt gatgtaaaag tttctatatt tgtaagtcaa ttaaaggata
     1501 caggaaatct aaataaacag gagtagcatg aagtcaatgt agctcctgtt ttcaattgat
     1561 aaccttagaa cagctgaagg agaatgtctc gttttaaaag attgatttag tagttgggcc
     1621 aaaataattc attatttgct aattaaataa ctatttcgta ttgacagaag caatttaatt
     1681 atctataatt attgatgttc cgctgattct tgtcatagta gctcagctgg atagagcatc
     1741 cgccttctaa gcggacggtc gggggttcga atccctcctg tgacgctaaa aaaccgttag
     1801 aaactgaggt ttctaacggt ttttctttgt tttttgtgcc aatattgttt gttttcaaag
     1861 gcaaataaaa taaaaaaaca tccttatcga gtacaattat aaggagcaaa aaaataaaac
     1921 tcatcagaaa agaggaagaa gatatgacga tactatctag aattt
//
LOCUS       DACEKH010000192         1889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEKH010000192 DACEKH010000000
VERSION     DACEKH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.202"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            273..1751
                     /locus_tag="IW662_002711"
     CDS             273..1751
                     /locus_tag="IW662_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0867496.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          694 a          261 c          331 g          603 t
ORIGIN      
        1 aaagagcagg acggtggccg tcctgctctt ttttgtggtt gtttacgcaa atggcgcaaa
       61 tcttgtacat tttaacacaa taaaacaaaa aatatagaat atttcctcat aaatttttcc
      121 attttttgca atattctcaa aaaaaagata aaaaatgaga ataactgatg aaaaaacaag
      181 cataatccat cataaaatca gtataggtga aaagatgaac tttcatctgc atatcaagta
      241 attggtatga ggcatttttt gaggtgtagg atatgaagtt ggattacctt atagaagagg
      301 aagatagaga aaaactgctg ctagctagat atttagaatt tgagaaaaag aattatgtag
      361 tgctgcctaa gttatatgaa acattaggtt tgtctaaatt taaaacaaga aattatatca
      421 tagaattaaa taaagaattt actgaattca aaacaaagcc agagatccaa atttcagaag
      481 aagcagagat ccatgtcgaa cgtctgaatt tgacggatat caaaaattta agaagaatct
      541 actttcaacg atcaaaaatt gctcgactgt taacggagat cgtggattca aaagtaacga
      601 tcgaaaaatt tgcagagaat cattttttaa gcatatcaag agcctatgta aaaagaagag
      661 aattagtctc ttttcttgaa cagcatcaaa taaagttaaa gaaaaacata ttagttgggg
      721 aagaaataaa catacggaac gtattatttg gggtatattt cggggtcttt aatggttttt
      781 ctatgccatt tagagaaagc gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat
      841 atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa attcaatttg ctaaccacta
      901 ttagtttgtt gagggcctat gggaatcaac caatcggaaa acgtttcttt tcagatgaat
      961 actttaagag ctcagaagga atgaagatca tcaaggattt atcacatatt tcttttcttc
     1021 atatccctaa agagcagatc attgatgaga tttcttatat catgttgttt ttgatcacgg
     1081 aagaagaatt tgaattagat ggcgcatttg gacttgcgga agctcatttt catgatatcc
     1141 acatgattag tcaaaaaatg tcagtggaat tgattgatca attaccattt tcgattgaaa
     1201 tctctgaaga agaaagacta tatatacgaa aagaacttcg tatgggtatg atgcatgtca
     1261 atcgaaaaaa ttggatattc gactttgaga tgactagctt tacgtctaaa aagcagctac
     1321 agtttttctt cgagacgtat ccaatattca gtacagcaat ccaaaaggtc atacaagaga
     1381 ataaacgcta tttcttggga aaagatgaac gtttcatgat tcgcttattt tatgattatc
     1441 tctttttatt ggtcggtatt tgtccggtat catattttga agcaccgatt catgtctgta
     1501 ttgatttttc gcaaggatct gcttataccg attacattat ttctcaagtg aaaggattta
     1561 aaaattacaa tattgtgatt gagagtcgaa tcacttctcg tacgaatatt tatttatctg
     1621 attgtttact tgaaaaattt aataaagaac agataatttg gaaaaatcca ccgactccag
     1681 atgactggga atattttgga aataccatca tcagagaaaa aaatagacat ttaactgaaa
     1741 aaatcagcta gtatttatgg atggcagctt tgtctcacat atttattagc tgatttatgt
     1801 ttttttcaaa gaaaaggtaa ctagatataa ctagatataa ctagatatat tacttaacaa
     1861 aattaaaaag tacataagtt attattccg
//
LOCUS       DACEKH010000193         1889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEKH010000193 DACEKH010000000
VERSION     DACEKH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.203"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            243..809
                     /gene="amaP"
                     /locus_tag="IW662_002712"
     CDS             243..809
                     /gene="amaP"
                     /locus_tag="IW662_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0867497.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            822..1010
                     /locus_tag="IW662_002713"
     CDS             822..1010
                     /locus_tag="IW662_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0867498.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1023..1580
                     /locus_tag="IW662_002714"
     CDS             1023..1580
                     /locus_tag="IW662_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0867499.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1612..1854
                     /locus_tag="IW662_002715"
     CDS             1612..1854
                     /locus_tag="IW662_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0867500.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          691 a          293 c          391 g          514 t
ORIGIN      
        1 cataatcttt gcaattttta atcttgaata atatccttta agtatagcca gcaatcaaaa
       61 gtaaatagag aataagtaag caattgttat ttcagtatca gactggcaat aaaacgccgt
      121 tatgatccag caatgtttgg tagcgcttta tttactgatg ctatgctaac tgttcgctat
      181 ctatactgtg attgtcggta gagttattta cagggtaaaa ataagtttat aggaggtgga
      241 aaatgaataa ggggttaaaa ataatcggtg ttatcatgag tttactgcta ctttcagtat
      301 ttctagcgat tgcgttgatc aatgcgccgt atattatgcc tgaacaattg gaacgatttc
      361 gcttctttac tttaacgaat tactacatgc aacaatatat tttctgggca gctgtagtat
      421 ttgccgttct tgctattatc atcttgctag tcgttctttt ctatccaaag tcaagaggaa
      481 ctttcgttat gaaacgggaa gatggcaaac taacaatcga caaaaaagcg atcgaagggt
      541 tggtacgttc ccatttacac gaagaagagt tcattcattc acctaaagta cggatacgtt
      601 caacgaaaaa tcgaatccat atccatgtca acggagactt gaaacgaact tcttcattag
      661 tagggaaaac aggtgtattg atgcaagata tcgaagaaga agtaactaaa gtactcggaa
      721 caaaagaaac ggtcaaagtg gcagtcacat acagcggtta tgaagaacaa gaagatacaa
      781 gagactacaa gcactctcga gtggaatagg agggccaaat aatgaaagaa ctattttcag
      841 catacaagct ccctattatc tttgggggtc ttggactact gctagctgtt ttatggttaa
      901 cgattggatt tttcaagaca ttgttgatca taatcatgac agcaattggc gcaggaatcg
      961 gtttttattt gaaacagtca ggaatagttg atcaattttt taaatcatag gaggaaacaa
     1021 agatggaaaa caaaaacatg aatacaacaa atggatcagt tttggcaaat gaaaaaggag
     1081 aaaaccgcat gatgaatggt gaattgactt atgaagacaa agtcatccaa aaaatcatcg
     1141 gttatgcatt gaaagatgta gatggtctat tgacattaga tggcggattt ttctcaaata
     1201 tggctgaaaa attagtcaac acagataacg taacagcagg tatcaataca gaagtaggta
     1261 agaaacaagt agctgtggat ctagatgtga tcgtagaata cggtaaagac atcgaagata
     1321 tttacaacca aatcaaagaa ttgatcagta ctgaagtaaa caatatgaca catttagatg
     1381 ttatcgaagt gaatgtgaat gttgcagaca tcaaaacaca agaagaatac caaaaagatc
     1441 aagaaacagt acaagacaaa gtaacagaag cagcaaaatc cacaggacaa tttgcttcac
     1501 gccaaacaga taaagcaaaa tctgctgtag gcaaaggtgc acaaaaagtc aaagaaaata
     1561 acgaaccacg tgtacaataa gaagtaagaa gtcagttagg aggaatttaa aatgggattt
     1621 atttgggcat taatcgtcgg cggtatcatt ggagcaattg caggagcaat cacgaataaa
     1681 ggatcatcaa tggggatcat cgcaaacatc attgctggtt tagtaggttc agcaattgga
     1741 caagctttac taggaacttg gggaccaaca attgcaggaa tggctatcat tccatcattg
     1801 atcggtgcaa tcattttagt attagtcgtt tcattctttg cacgtaaatt ataagcaaat
     1861 tgaagaaaaa cttatgttga aatgaagaa
//
LOCUS       DACEKH010000194         1750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEKH010000194 DACEKH010000000
VERSION     DACEKH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.204"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(190..567)
                     /locus_tag="IW662_002716"
     CDS             complement(190..567)
                     /locus_tag="IW662_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867501.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(867..1748)
                     /gene="galU"
                     /locus_tag="IW662_002717"
     CDS             complement(867..1748)
                     /gene="galU"
                     /locus_tag="IW662_002717"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0867502.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          501 a          381 c          254 g          614 t
ORIGIN      
        1 acattggtat gatttatata tatataaata acataaaaaa aggaatggct tatcccgata
       61 caacaagcat tcctttaaaa aagattcttt tatataagtg aaaaaagctt ttttccacca
      121 attaaataaa tctctcatct caatagttta tccattattt tatctttttt tgcgtatacc
      181 tactaaatat taccgtcttc ttctcgttct tctactttgt tcgagagaat ctgtactaga
      241 aaaagtagtt gtatgtctac cttttctgcc tatccgagac tttcttttca tatatgggat
      301 ataaatgatt agatacagta cagcaaaaaa taaagcaaat acgcaagctt cccaactacc
      361 taacgccaat tcaaccatca aggtagcaac cccaacggat gctgcatacc agataaaaat
      421 cagaaacgtt tctttttttc tgaccatgcg aaacatgata aatagactaa taagcaatag
      481 tggcaacaca aatatccaat tgcttccttc catcagccac ttgagtatat caagcagcga
      541 ctgtgcaaca gcgactaatt ttcccatagt gaacacacac ctttttttat aattaaaaaa
      601 ttcgcctttt tattctacta tgaatcttct ggcttggctt tcatttctct tatgtaatca
      661 ttttctttta tacaacttaa aaaaaaatta aaccattagc ataatatttt ctgaatctat
      721 tcacgaaagt ctttttctat ctttcgtttt ccacgactca actgatctag agtcaaaaaa
      781 ctaaaacgac agttgcgcaa atgacgcaca agctgccgct ttaataacaa atagcctttc
      841 attctcagca ggccacttat ttccagttaa ctttctgttc ttagcttttt actcaactgg
      901 ataatatatg cacttaatga agtcccaatt tccgaatgtt gaagtccata ttcaatattt
      961 gtcttcatat atcctaactt atcgcctacg tcgtatcttt ttcctttaaa ctctctcgca
     1021 tagaccgcct ctgtctggtt aagagtctga agtgcatccg ttagctgtat ctcgtttcct
     1081 gctcctggtt cttgagtttc taatatctca aatattttag gagtcagtag ataacgtccg
     1141 ataatggcta aattacttgg tgcttttccc atttcaggtt tttctacaaa cccttttact
     1201 ctgcaacgac ccttttcgct atctgtttta ggatcaataa ttccgtattt agatgtttca
     1261 ttttctggta cacgcatgac ggcaacagcg gaagcttgtg tttcttcata atccatcatc
     1321 aattgttgag tcaatgggat atcatctttc atcaaatcat cccccagcat gacgacaaac
     1381 ggttcatttc ctataaatgc ttttgcttga agcacggcat cccccagtcc tcttggatga
     1441 gattgacgga tgaagtgaag attgacttga gtcgtttctt caactaaagc gagtaattct
     1501 gttttttcct tttcccgcaa attgttttcc aattcgatat ttgcatcaaa gtgatcttca
     1561 atgggtcttt tttcttttcc cgttacgatc aagatgtctt cgattcccga atccaatgct
     1621 tcttccacaa taaactggat cgttggttta tccacgattg gaagcatttc ttttgccatg
     1681 gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg caggaatgac tgcttttttg
     1741 accttcactt
//
LOCUS       DACEKH010000195         1749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEKH010000195 DACEKH010000000
VERSION     DACEKH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.205"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(14..589)
                     /locus_tag="IW662_002718"
     CDS             complement(14..589)
                     /locus_tag="IW662_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0867503.1"
                     /translation="MKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDDN
                     PTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIAN
                     YKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYV
                     MMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
     gene            670..>1749
                     /locus_tag="IW662_002719"
     CDS             join(670..946,946..>1749)
                     /locus_tag="IW662_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0867504.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT          595 a          317 c          327 g          510 t
ORIGIN      
        1 aggggggaat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaacgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgaag tagttggatt atcatccgta aaaccatgag
      481 ccaaatcata ggctagtttt tctttactta cacccatttc agaaagataa ccgcaaggat
      541 ctgtatgatc gccccaaata ttttgtgtta cccataaatg cgatttgatt cctgtggata
      601 ggctacaatt aactagacag aaaaattaag gtgtgtagac tagaagaaaa cataccagga
      661 ggaattttta tgtctaagag aacacgaaga actttttcac aagaattcaa gcaacaaatc
      721 gtcaatcttt acttagctgg aaagccacgt gtagaaatca ttcgagaata tgaactaacg
      781 gcttcagcat ttgacaaatg ggtaaagcaa tctaaaacga gtggttcatt caaagaaaaa
      841 gataatctta cgcctgaaca aaaagaattg ttagaactac gtaaaagaaa ccagcaatta
      901 gaaatggaaa atgatatttt aaagcaagca gcgctgatat tcggacgaag agacaagtaa
      961 tcgatgcgaa taagcatctt taccctatat cagcgatgtg cagaatatta ggtctatcac
     1021 gtcagtccta ttattatcaa tcaaaaccaa agaaagacga atcagaactt gaagaagtag
     1081 tcgctgaaga atttatccgc agccgaaagg cctacggctc aagaaaaata aaaaaagcct
     1141 tatcaaaacg aggcattcag atcagccgac gaaaaattag tagaatcatg aaaaatagag
     1201 gattaaaatc gagctatact gttgcttatt ttaaagtaca tcattctact tgcaatgaag
     1261 ccaaaacgac aaacgtattg aatcgtaaat tcttaagaga caacccatta gaagcgatcg
     1321 taacagactt gacttatgta cgagtcggga aaaaatggaa ttatgtctgt ttcattttgg
     1381 atctgttcaa tcgagaaatt ctcggctatt cttgtggaga acataaagat gccgttctag
     1441 taaaaaaagc atttagccgt atcaaacaac ctctgacaga ggttgagatt tttcatactg
     1501 atcgtggaaa agagtttgat aaccaagcta ttgatgaatt attaacaact tttgacatca
     1561 atcgatcatt gagtcataaa ggctgtcctt ttgataatgc cgtagctgaa tcaacttata
     1621 agtcgttgaa agtagaattt gtctatcaat acacatttga aaccttacaa caattggatt
     1681 tggagttatt tgactatgtc aattggtgga accaccttcg gttgcacggt acacttggct
     1741 acgagacac
//
LOCUS       DACEKH010000196         1745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEKH010000196 DACEKH010000000
VERSION     DACEKH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.206"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            131..505
                     /locus_tag="IW662_002720"
     CDS             131..505
                     /locus_tag="IW662_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0867505.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IW662_002721"
     CDS             complement(619..1248)
                     /locus_tag="IW662_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0867506.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IW662_002722"
     tRNA            complement(1382..1454)
                     /locus_tag="IW662_002722"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IW662_002723"
     tRNA            1668..1741
                     /locus_tag="IW662_002723"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
BASE COUNT          603 a          256 c          306 g          580 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaa
//
LOCUS       DACEKH010000197         1712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEKH010000197 DACEKH010000000
VERSION     DACEKH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.209"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(168..1460)
                     /locus_tag="IW662_002724"
     CDS             complement(168..1460)
                     /locus_tag="IW662_002724"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ0867507.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          490 a          362 c          295 g          565 t
ORIGIN      
        1 gtttttgtca cagtccctta ttctgaataa acagtgttca agagacagcc tatcggcaat
       61 aaggctaaac gtctctttca ctacctcttc aagttattat tatcgtatag ataaacactt
      121 cgactcgctc tggtaagaac gtcctgattc agtcgtcgtt tttatcctta gatttgcgcc
      181 cagacgcttt ctaagatgtt tgtttgtgtt ctatcaggtc cgacagaaaa tgttgagata
      241 cgtacaccaa caagctctga gatacgacgt acatagttac gtgcgttttc tggcagttct
      301 gcaagtgttt tgcatcctgt aatgtcttct gaccagcctg gcaattcttc atagattggt
      361 ttgcagcggt tcaactcttt caagcttgct ggatagtggt agattagttc gccatctaat
      421 tcataagccg tacaaatttt tactgtttcc aatccactta atacatcgat cgagtttaaa
      481 gataggttgg taatacctga aactcgttta gaatggcgca taactacaga atcaaaccag
      541 cctacacgac gcggacgacc agttgtcgta ccgtattctc gtcctacttc gcggatttgt
      601 tctcctgttt catcaaataa ttcagttgga aatggtccat caccgacacg tgacgtatag
      661 gctttgcaga cgccgacaac tttattgatt tttgaaggcc ctacaccgct tccgattgtt
      721 accccaccgg ctactgggtt agaagaagta acaaaaggat aagttccctg gtcgatatcc
      781 agcattacac cttgtgcgcc ttcaaacaac acgcgtttgc ctgcatccaa agcatcattc
      841 aagataacgg aagtatctgt gacgtattgt ttgatttgtt gtccgtattc ataatattct
      901 tcaaaaatat cgtcaaaatc aagtggttct gaatcaaaca tttttacaaa ttgacggttt
      961 ttgtcatcta gattcaaacg aagacgttct tcaaaaattt ctttatctaa taagtctgca
     1021 atacggatac ctacacgagc tgctttatcc atataggcag gtccgattcc tttaatcgtt
     1081 gtaccaatct tattttcgcc cttagcatct tcttgtaatt ggtccaattt gatatgataa
     1141 ggcaagatta cgtgcgcgcg gtcagaaata cgcaaattac ttgtttctac acctttttca
     1201 tgtaaataag ctaattcttt aaccaatgat ttaggattga ctacaacgcc gtttccgatc
     1261 acactgatct tgtctttata aaaaatccca gaaggaatca aatgcagttt ataagtcaca
     1321 ccgttaaact taattgtgtg ccctgcattg tcgccacctt ggtaccgtgc aatcacttca
     1381 gcattttcac tgagaaagtc tgtgattttt cctttccctt catcgcccca ttgcgtacct
     1441 actactacta cagatgacat gtgaacacct cgttctatat tatttaatcc tgaaccattg
     1501 tatcaataat agatacatat ttcaaataaa atcgaatgat ttttattttt attttataaa
     1561 aacgcttaaa aaacgaactt tatcttttta gaatggttat taaaaaagag gttgtaacag
     1621 aagtggtcag cttcaagaaa taagaaggaa tgtacgaaaa ttgcttctca aatttttgtg
     1681 aaatttcgac ttatttttcg aagaagctac tt
//
LOCUS       DACEKH010000198         1707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEKH010000198 DACEKH010000000
VERSION     DACEKH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.210"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..368
                     /locus_tag="IW662_002725"
     CDS             <1..368
                     /locus_tag="IW662_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0867508.1"
                     /translation="IDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAI
                     YFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYR
                     TPLEVFLSYVSIDDLSNLI"
     gene            501..707
                     /locus_tag="IW662_002726"
     CDS             501..707
                     /locus_tag="IW662_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0867509.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            718..>1707
                     /locus_tag="IW662_002727"
     CDS             718..>1707
                     /locus_tag="IW662_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0867510.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDK"
BASE COUNT          589 a          277 c          353 g          488 t
ORIGIN      
        1 cgattgatat tgaaaaaaaa ttaaatcaat ggtttgaatc tgtaccgaaa aacctattca
       61 aatccatcac ttttgattgt ggaaaggaat tttcaaattg gaaacagatc agtaatgtca
      121 atgatattgc catttatttc gctgatccag gaacgccgtc tcaaagaggc ctaaacgaga
      181 attctaacgg attgttacgt agagatggtt tattgaaatc tatggatttc aattcagtag
      241 atgaattttt tattcaatct gtcgcatcta aacgaaataa tattcctaga aaatcactgg
      301 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaact
      361 taatttgaca attaagaaag ataaaattga acaactggac gggcaaattg atgccttgct
      421 ttcagaaaga gaaatattga tagaagaaaa caagcgattg aaaaagaaag tagcccaacc
      481 aatgaaggga ggtggatagt atgaacaata gaacctttga tattttgaaa tggatcgcta
      541 tagtagttat ccccgcttta gcgacttttg tcggtcttgt cggaaaaggt gttaactggc
      601 ggtacacaga tataacggtg atgattatta caggttttgg agcatttcta gggagtattt
      661 taggtgtatc aaatcgaacg tataagaaaa tttcatcaga taaataggag gatagatatg
      721 aaaaagaaaa ttactattac tgcgatgagc ctgttaacgg ctcttttttt attgccaatt
      781 aacggatttg cctatactat caacaatgaa tttaatttgg gcgcaaatga aggtagctca
      841 caagtagcaa ataatcagta tattttactg catgaaacgg ctaatgaaac agcaacagga
      901 cgcaatgaag cgcagtatat gcaacgttca tggactagcg cttatactgc ttatattgtg
      961 ggagacggcg gaattgttta tcaagtcggt caacctggtt atgtacagta cggtgctggt
     1021 tcgtatgcta atgccaacag tcctgtgcag attgagttac aacacacaca tgataaagca
     1081 acgtttgaga aaaactacaa ggcatacgtt gaattggcta gagattcagc aatgaaatat
     1141 ggtattccat taacgttgga cactccttat aaccaaccgg gaatcaaatc gcatttatgg
     1201 gtaacacaaa acatctgggg cgatcataca gatccttacg gttatctttc tgaaatgggc
     1261 gtaagtaaag aaaaattagc atatgattta gctcatggat ttaccgatga aaatccaaca
     1321 acttctgaaa acaagcctgt cattgatcca acacgagctg gtgcagctaa tcctacactg
     1381 acagatggaa cgaattacgc ccacattgat cagtttggag aaatcgaaaa tgcaaatttg
     1441 catgtagctg gatggcacat tgctaactat aaatacgagt atatcttcat tatggattac
     1501 aatactggga aagaattagc tcgagtaaga gctgatggaa tttatagatc agatgtaaat
     1561 caagcttata atacttctgg aaatgttggc tatcatgtat cttttaacat gcgtaacttc
     1621 cctaataaga aagtctatgt catgatgcgg gcaacgaatg atccagaggg aaacactaaa
     1681 ggcggtgcgc aagatttcca tgacaaa
//
LOCUS       DACEKH010000199         1700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEKH010000199 DACEKH010000000
VERSION     DACEKH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.211"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            466..1065
                     /locus_tag="IW662_002728"
     CDS             466..1065
                     /locus_tag="IW662_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0867511.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1129..>1700
                     /locus_tag="IW662_002729"
     CDS             1129..>1700
                     /locus_tag="IW662_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867512.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT          612 a          257 c          321 g          510 t
ORIGIN      
        1 acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat aatgatgaaa tcatttagtt
       61 cttataaatt agaaaagaga aaataaatat tccatatcta ctttattgga tccgtaacat
      121 gactaaattc tgaatacttt ttaaagctta tcatttggta aaactgaata taagttaatt
      181 atatagactg aaaattttga attttcagaa tagaataact taaaagtact gacaaaattt
      241 tttaaagggg caatattaat gttctgagat aatcaaatcc taccgaaaat atcaaaaatc
      301 caatgtgaca caatcagatt gtgtctcatt cgagggaatc acacaatccc acgtggctct
      361 aggcagaatt gaatttttgc ttggggcttt ttccttatat tgattttgaa tgagacttat
      421 tatatgttat aatgcaatta agtatcattg gagagggcga agaaaatgaa gacggtagaa
      481 cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa tcttggcttc gggaaaatat
      541 ggccaacgaa atttggttct tttttcgatt gggatcaaca ccgcctatcg catttctgat
      601 ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac gcggtcgcgt ggtggtcaaa
      661 gatcgattag cgatgaagga acaaaaaaca gctaaacata attcggtcta tatttccaac
      721 aaattgcgaa aaacgatttt ggagtacgtt caacaggaat ttcctgatca actccttcag
      781 caagattttg acggttattt gttccctagt cgaaaaggga cgaatgaacc gctcaaccgc
      841 caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa tcggattgaa agacattggc
      901 ccacattcca tgcgaaaaac gttcggttac tttctctata aacaggggac caaaacagaa
      961 atcattcagt ccttgttgaa ccattcttct cagcgcgaaa cattgcggta tattggtatt
     1021 acacaagaag acaaagatac agctgtaaaa agcttagatt tataatataa aagttaagtt
     1081 taaacatgga tagttttaaa ttagtagatt gagtgttggg aggaaagaat gagtacatta
     1141 tatattatag gaaacggttt tgatttaagt catggtttaa aaactagata cactgatttt
     1201 tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata aaatagacac aatgataaat
     1261 gaaaataatg aggtgagaag tacagctgat gataagatag gttggtttaa ttttgaaagt
     1321 agtttgcatg agttaattca aagtccagtc tttgaagatt atgtagaaga gtctaaggat
     1381 tatctcatag gttatggaca tgaagattgg tcagattctg cccatcatga ttttcaaaat
     1441 atggtttatg attatgttgt ttctctagaa gaattacaac aacttttcaa ggaatggata
     1501 cgcaaagaaa tacagccgac aatagaacat aaaattccaa aataccaatt ttcgtccgat
     1561 gtactattct ttacattcaa ttatacagat gttttggaaa ttctatacaa gcaaccgaaa
     1621 gaaaacatat atcatattca tggcagttat gacgatataa taatcggcca ctccgagaat
     1681 tttgacaact ttataggaga
//
LOCUS       DACEKH010000200         1655 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEKH010000200 DACEKH010000000
VERSION     DACEKH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.212"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            200..742
                     /locus_tag="IW662_002730"
     CDS             200..742
                     /locus_tag="IW662_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867513.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="IW662_002731"
     CDS             898..1347
                     /locus_tag="IW662_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ0867514.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
     gene            complement(1377..>1655)
                     /locus_tag="IW662_002732"
     CDS             complement(1377..>1655)
                     /locus_tag="IW662_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0867515.1"
                     /translation="GITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLE
                     LFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          624 a          236 c          319 g          476 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tccgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attcc
//
LOCUS       DACEKH010000201         1578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEKH010000201 DACEKH010000000
VERSION     DACEKH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.213"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..1297
                     /locus_tag="IW662_002733"
     CDS             128..1297
                     /locus_tag="IW662_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0867516.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          566 a          254 c          258 g          500 t
ORIGIN      
        1 atatcccata ttttaaaata aaagatagcg taaaatattt tgtgtaaata gaaatttagg
       61 gtagaaaaaa aagtccattc tgtaaaatta aagttaccac acagaaattt tataggagga
      121 ctttctcatg actaaattta ctatagaaat tatgcaaaca ctactcaata aaggggattt
      181 agatgaatta tttcaggatc atttagagcg agcgattaat tcacttctac aagcagagct
      241 aactgctttt ttagattacg aaaaatatga tcgaaatgga ttcaattcag gaaattcccg
      301 taacgggaat tattcacgta catttaaaac agaatatggc gagttgaatt taactattcc
      361 tagagatcgg aatggcgaat tttctcaaca aacactacca gcatataagc gaaccaatga
      421 ttcattagaa acgaccatta tccaattatt tcaaaaaggc attactatgg ctgaaatctc
      481 caaattaatt gaaaaaatgt atggtcacca ctatacgcca caaacaatct ccaacatgag
      541 taaattggtg gctgaagatg ttttagcttt taaagaaaga acgttagaag ccaattattc
      601 cgttatattt atggatgcga cgcatatccc tgtgaagcga cagactgttt cgaaggaagc
      661 agtgtatatt acgattggta ttcgtttgga tggaaccaaa gaagttcttg ggtttactat
      721 tgccccaact gagtctgctt atatttggaa agaagttctt caagatctta gaaaacgtgg
      781 gttagaagaa gttttattag tagtgacaga cggattaagc tgtattgaag aaagtatcca
      841 tagtgtgtat ccgaatgccc aatttcaaca atgttgtgtg catgtatcta gaaatatcgc
      901 tcataaagtt cgtgttcgag gtcgaaaaga aatttgtgag gatttcaaat tggtttacca
      961 agcgaattca aaagaagagg cattggatca catcgacttt atgactagga aatggaaaaa
     1021 gcagtatcca agagtcgtca atttactctt gaatcctgcc ctattaacct tttataattt
     1081 ccctcacgcc atcagacgaa caatttattc gacgaacctg attgaaggct ttaataagca
     1141 gctaaaacga tatactcgaa gaaaagaaca attccctaat gaagaatctc tagagagatt
     1201 cttcatttct caatttaatc aatataacca aaaattttta ggtagaattc acaagggatt
     1261 taaagaaatt caggatacat tagagtcgat gatttaactg taattaacag aatggatttt
     1321 ccatttacat ataattcttg acgctaccat aatctaaata accatcatat tcatctacta
     1381 cttatataat tgagttttat taaacttttt aagtttctat tctatcaaat atttaaacaa
     1441 tctctgcatc cgaaaagact acctcgtata gttttactaa caatattttt attagcgtgt
     1501 tcaaatcctg atttcttgta tcaattattt tctgttcact ttcaataaga aaaaagtctt
     1561 tacttccatg gataataa
//
LOCUS       DACEKH010000202         1538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEKH010000202 DACEKH010000000
VERSION     DACEKH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.214"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(137..427)
                     /locus_tag="IW662_002734"
     CDS             complement(137..427)
                     /locus_tag="IW662_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867517.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="IW662_002735"
     CDS             complement(545..805)
                     /locus_tag="IW662_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867518.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="IW662_002736"
     CDS             complement(942..1355)
                     /locus_tag="IW662_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ0867519.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          572 a          247 c          228 g          491 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggt
//
LOCUS       DACEKH010000203         1527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEKH010000203 DACEKH010000000
VERSION     DACEKH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.215"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..220
                     /locus_tag="IW662_002737"
     CDS             <1..220
                     /locus_tag="IW662_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0867520.1"
                     /translation="FVVLDKLVLTEEKGLYQEKKQVREYLIDNFFFIIKNKRLTFQRK
                     ISMLLLTFGIAAYKIPVKYQYKKVKAVN"
     gene            291..>1527
                     /locus_tag="IW662_002738"
     CDS             291..>1527
                     /locus_tag="IW662_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAQ0867521.1"
                     /translation="MFIYILRIVIQFVGRSFFIYFLGAKYLGLNGLFTNILSFLSLAE
                     LGIGSSIIYSLYKPLAQKDESKVKALMNLYKKTYEFIGIFIGIVGVLIIPLLPLFIKN
                     STDIPHMYRYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAINDFAFLFFMNIVQVIA
                     LYFYSNFTLFLIVQILFTLFSNINISRTVDKRYSYLKEKDVVSLDKQTKDEVKKNVIG
                     NMSSKIGGVIVMGTDNILISTFVNLTAVGIYSNYSLIVTSVQNLCKQITNSITASVGN
                     FAVSNKKKDSFELFKKHFFINHSLIFFSSILLLTLMNPFISLWVGAKYMLPMSTTILI
                     VLNYSVQVYRNTSFVFIESFGLYWSQRKKPIIEAGVNLLLSLLLLIVFDMGINGVLIG
                     TIGSSLGFVIWYEAFIIYKT"
BASE COUNT          524 a          174 c          242 g          587 t
ORIGIN      
        1 ctttgttgtg ttagataaat tagttttaac tgaagaaaaa ggactgtatc aagaaaaaaa
       61 acaagtaaga gagtatttaa ttgataattt cttttttatt ataaaaaata aacgattaac
      121 ttttcaaaga aaaatatcta tgttattgct tacttttgga attgctgcgt ataaaatacc
      181 ggttaaatat cagtataaga aagtaaaggc tgttaattaa tgctttagtt aggatgaatt
      241 agatgaaaaa aagcaggttg gagtattctt taataaattc ttctatatca atgtttatat
      301 atattttaag gatagtaata caatttgtag gtagaagttt ttttatttat ttcttaggag
      361 cgaaatattt aggattaaat ggtttattta caaatatact tagtttcttg tcgttggctg
      421 aactaggcat aggctcttct attatttatt ccctatacaa accattagcc caaaaggatg
      481 aaagtaaagt aaaggcttta atgaatttgt ataagaagac atatgaattt attggtattt
      541 ttatcggcat agtaggagta ttgattattc cgctgttacc tctatttatt aaaaatagca
      601 cagacatacc acatatgtat cgatattatg tattattttt attaaattca gttcttagtt
      661 attttttcac gtataaaaga tcacttgcaa tagcagatca aaaaaattat ctggttgcga
      721 ttaatgattt tgccttttta ttctttatga acattgtaca agtaattgct ttgtactttt
      781 attctaattt tactctattt ctaatagtgc agattttgtt tactctattt tctaatataa
      841 atatttccag aacagttgac aagagatatt catatttaaa agagaaagat gtagtttctc
      901 tagataagca aacaaaagat gaagtaaaga aaaatgtcat aggtaatatg tcctcaaaaa
      961 taggcggggt tattgtaatg gggacagata atatattaat ttctacattt gttaatttaa
     1021 cagctgtagg aatttattct aactattcat tgattgtcac aagtgtgcaa aatttatgta
     1081 agcaaattac taattctatc acggcaagtg taggaaattt tgctgtatca aataaaaaaa
     1141 aggattcatt tgagctattt aaaaaacatt tttttatcaa ccactcacta atcttttttt
     1201 cttctatatt gttattgact ttaatgaatc ccttcattag cttgtgggta ggagccaaat
     1261 acatgctacc tatgtcaacg actattttaa ttgttttaaa ttatagtgta caggtttata
     1321 gaaatacatc cttcgttttt atagagtcat ttggtttata ttggtctcaa cggaaaaagc
     1381 ctataattga agcaggtgta aacttgttat tatctttgct tttgttaata gtttttgata
     1441 tgggaatcaa tggagtactt atagggacta tcggatcgtc tctaggattt gtgatttggt
     1501 atgaagcatt tatcatatat aaaacgg
//
LOCUS       DACEKH010000204         1515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEKH010000204 DACEKH010000000
VERSION     DACEKH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.216"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..980
                     /locus_tag="IW662_002739"
     CDS             6..980
                     /locus_tag="IW662_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0867522.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          527 a          193 c          248 g          547 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta
     1381 gagtaattaa ttagatgttt attttatagg actgatatta tagattacaa atagtatcgt
     1441 atttcgcaat cttaaaatta tcttcttact atgaataaga taataaaatt tgtatttgtc
     1501 ctttttaggt aactt
//
LOCUS       DACEKH010000205         1511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEKH010000205 DACEKH010000000
VERSION     DACEKH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.217"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(479..1093)
                     /locus_tag="IW662_002740"
     CDS             complement(479..1093)
                     /locus_tag="IW662_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0867523.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          494 a          287 c          244 g          486 t
ORIGIN      
        1 taataaacac ttataaatat ccattatcta tccttcaatt tttgtgtatt ttatttctat
       61 tagaaaaagg tatcctgttg gacaattttg ttatatcagg taaacttcat tgcaataatg
      121 ataacaaatg tactataatt actttgtaaa gagttttggg gaagactctt tcatcaacgt
      181 gtattaagag acaagaaaga atccatatgg attctttctt gtctcttttt ccactacttt
      241 ttatttttct gacactacat attcttataa ctcatttctc ataactgtga aacaacttat
      301 aaaaataaca aactttaaac aaaacaagct taaaatcaaa gatgatccat cttaaaataa
      361 ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata atcgttttct ttttttcaaa
      421 cgctccattt ttatgtggaa cattattttt ttaaggaact ttgtcaacat gtggataact
      481 attttccact tgtgtcctgg actagctgtt ttgctgtaaa atacaaaact tgctgcagca
      541 aaaagatcgt tgcaagagag gctgttccgc agatcgctgg atctagattt ggcaaagtca
      601 agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa atcagcaaat tcaaataatc
      661 gcaaaccact gctatgttgc gatttgatac ttcgtgacaa atcaaatcca gtaacaatga
      721 ttaatggatg accattttct ttgatccact gagctagttc gatgattgcc gggtttcttc
      781 cttcattcga aagaagaaga aaaatgtctt ctgatttgac agaaagctga tcaataattg
      841 cctgtcctgc accttctagc gtttcataga cacctccggc tggattttca atcaatatac
      901 ttggtacaag atccggggtt tgatcccaaa attcaaatgc tacaccactt agtgtgcggc
      961 tagcatatat ctgcaatcta ccaccttgat taatttgttt cgcgatccaa gtgccagctt
     1021 gaagcatcag cggctcttct atccttaatt tctcttccaa ttggtgataa tagtcaaaat
     1081 aacataaact catttatctc tcccgcctag ctatacttga catgaataga aacagctgcc
     1141 taatctaagg aagtacataa aaaaacagta gttagacagt aacacatgtg gtctttgctg
     1201 tttcaaacac aaaaaaggca ttgaacgaat acgcccaata ccttgacata gcaaataaaa
     1261 caacgtgcag accatggaaa atatctacat aggggtagaa ttttcaccct agattaggta
     1321 caataaatta ggtcgatgca ctgcattgac tatggcaaaa tgatcggtca gggtgtatgt
     1381 gtgtgactgg tcattccctg attcttagcg ccaactaagg gtcagggagc ctttttttat
     1441 tcagctgttt ctatctgaat ttattgtaag taatctctta tccaagtgca agcaagaaaa
     1501 tacaaaaaaa c
//
LOCUS       DACEKH010000206         1488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEKH010000206 DACEKH010000000
VERSION     DACEKH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.218"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            47..1414
                     /gene="nhaC"
                     /locus_tag="IW662_002741"
     CDS             47..1414
                     /gene="nhaC"
                     /locus_tag="IW662_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0867524.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT          389 a          283 c          328 g          488 t
ORIGIN      
        1 agttttttca tggcagggcc ttttcatttg aaataggaga tcgacgatga ataaaaatat
       61 ttctttaaaa gaaagtatcg ctgtattgat cgtgttgttg gcactcctag gagtactgat
      121 catcggattc cagctctcgc cacatatccc gatccttgca gctttcatgt gtttgttgtt
      181 ttatggcaga tttaaaaagt tcacatggga tgagatccat gacgggatca tcaaaggaat
      241 cactccaggg atcattccaa tcctgatttt tcttttgatt ggtgtcttag ttgcttcatg
      301 gattttatct ggaaccatcc ctacgattat ggtttatgga tttaaacttg tttctgtgcg
      361 tttcttttta ccgacagcat ttcttgtttg tgcaattgtc ggagtgactg tcggcagttc
      421 atttacaacg atttcgacga ttggaattgc ttttttgggg atcggtcatt tacttggatt
      481 caatgatgca atgactacag gcgcagtcgt ttccggtgcc tttttaggga ataactgctc
      541 gcctttatca gatacgacca acttggcagc gggtattgga ggagttaatc tattcgaaca
      601 tatcgctggt atgcgttata cggatttccc agcattcgtc ctttcgttga ttttttatac
      661 gatattagga cataacagtc attcagccga tctagctagt atcaatgaaa tgatccaaaa
      721 catgaaagcg agcttttgga tctctccttg gacattgatt ccactagtta ttttatttgg
      781 gggggcaatc aaaaaaattc cggctatccc tacattacta gcgggatcga cagctgctct
      841 cgttctttct tttatccatg agaaaagttt gacggtcgga aaagcagcag acatcttgat
      901 gaatggttat gtcgcaaata cacctgatcc aaagctagat gaactgcttt ctcgaggcgg
      961 gatcatgagt atgttaggtt ctgctagttt gattttactt gcattggcat taggtgggtt
     1021 actgatcaaa tatctgatcg ttgataccat cgttcaagaa ttaaaagaaa agatggaccg
     1081 accttctcgt ttagtcgggt ttactgcctt gagctgtatc ggaatcaatc tgatcgttgg
     1141 agaacagtat ctgtccatca ttcttccagg agaaacattc aagcgttctt ttgatgacgt
     1201 tggcttatca aaaacctatt tgacacgaac actagcggat tcaggtgcga ccgtcaattc
     1261 tctcgtaccg tggggagtca gcggcacatt cattatggga acgatgaaag ttagcgcgtt
     1321 gcattacttg ccattcgcct ttttctcgat tcttgcacct gtctttacaa tattaggcgg
     1381 ttttttattg aatcgtcgac gtgtaaaaaa ttgaaggaag gaaaaaagag gctgggacag
     1441 aagcgtttaa ctccaagaaa taagaagaaa ctcaagaaaa ttgctttt
//
LOCUS       DACEKH010000207         1445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEKH010000207 DACEKH010000000
VERSION     DACEKH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.219"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..226
                     /locus_tag="IW662_002742"
     CDS             <1..226
                     /locus_tag="IW662_002742"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ0867525.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="IW662_002743"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="IW662_002743"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="IW662_002744"
     CDS             533..1336
                     /locus_tag="IW662_002744"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ0867526.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
BASE COUNT          462 a          259 c          344 g          380 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggaca
//
LOCUS       DACEKH010000208         1409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.220, whole genome shotgun sequence.
ACCESSION   DACEKH010000208 DACEKH010000000
VERSION     DACEKH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.220"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1359
                     /locus_tag="IW662_002745"
     CDS             <1..1359
                     /locus_tag="IW662_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0867527.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          466 a          255 c          299 g          389 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgta
//
LOCUS       DACEKH010000209         1402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEKH010000209 DACEKH010000000
VERSION     DACEKH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.221"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(648..848)
                     /locus_tag="IW662_002746"
     CDS             complement(648..848)
                     /locus_tag="IW662_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0867528.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          470 a          241 c          192 g          499 t
ORIGIN      
        1 gttttcagac gaaaattaat aatttctaaa tattctttaa aaaagcttat aactattatg
       61 ccttttttaa tatataacac agttattttt aaaaataaga atgctaaatc actctacaat
      121 aacggcgaaa gcacaaaaaa taaaaaaatt attacgttat taaaaaaaca gatgaaagat
      181 caggttttat tagatctcat tctttcatca cttgaaggga tttaatgaac tactttttcc
      241 tctttgtcgt ttttatatat tcgtattcct tcaaacagta ggatagcctc tttcaatgct
      301 tgatatttga actgtccgtt acttaaaaat ttagatgtgc cgccctcttc ctagaacata
      361 ttctttttta ctttttcatt aattgacaga ccaatcgaac gaaactgtaa atttcgatag
      421 gataatgatt ggtcaaactc attcattttc aagacatctc gattcatatt caagatagga
      481 atctttttat caacatcact ttattcgtat tttcctctac cataaaagct tcacgctcca
      541 caaaattgtt ttatacacta ctattcagcc cattcaacga ccaatgaatt ttttcgtaca
      601 aaaaaaccgt cttttctttc gaaaagacgg ttaaaagtca atttttatta agctttaaca
      661 atgtttgttg cttgagggcc acgttgacca tcttcaacat cgtaagacac tgcttggcct
      721 tcttctaaag ttttgaagcc gtcaccttgg atagctgaaa aatgtgcaaa tacatcattt
      781 ccatcttcac cagtgataaa gccaaaacct ttgtctgcgt tgaaccattt tactgtaccg
      841 ttattcataa ataaattcct cctgatacgt atcaatgtac gttttttcgt tgcaataaat
      901 atttgatagg caaaaggagt tttatctatg tagatatatc gctcaaatta cgtttcaaaa
      961 tagattactc atttaatata tcatgctttt tctaataaca caaggataat gcaatgaaat
     1021 ataaaattca ttgtcttcta ttgtcttcga atcgaagaaa aagttgaata accgcgattg
     1081 attcactttt tgttttcttg tttatttgct tttatctgca acttttgact ttaaaatttc
     1141 ttaactatga aaacctttca attggtggta tacttataaa tggaatttcg ttatcaacgc
     1201 gttcgttgcc tttcagcaag tatttaaaga aaggagaatg gctatgacat tctataacaa
     1261 agatatacaa tatcgagtaa ccttggatac agatttgaat ctatttgtcg tttttgacaa
     1321 agaaaatacc aatcatgtag cgacaggtgt tacgatcgaa caagcagtgc aagaattaaa
     1381 aaaatctgca taaggagtgg aa
//
LOCUS       DACEKH010000210         1397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.222, whole genome shotgun sequence.
ACCESSION   DACEKH010000210 DACEKH010000000
VERSION     DACEKH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.222"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            280..834
                     /locus_tag="IW662_002747"
     CDS             280..834
                     /locus_tag="IW662_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0867529.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1088..>1397
                     /locus_tag="IW662_002748"
     CDS             1088..>1397
                     /locus_tag="IW662_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867530.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT          564 a          147 c          241 g          445 t
ORIGIN      
        1 atttcatata atttaattat gtaaattagc tattctaaga acttctacgg ataactgccc
       61 gtctaaaatt taattaagag cttagaatct tcatataagt aaggttaatt tttttgtaca
      121 aaatagtcgg aatattctga gttactgaaa tgatataaaa tcataaacta aaccccttga
      181 ttttcttcac ttttaacaga gtatatatat aagttcatga ttatggtaaa atatatatat
      241 aatcatgaac tcagaatatt tttaagaagg gaaacaatag tgagctataa tgtacaaccg
      301 ttaaggacaa atgaagagat acaagatttt cagttttggc taagaagaac aagtaatcca
      361 gaaagagata gttttttatt tttgtttgga atcaataatg ggttacgtat gtcagatatt
      421 ataggattaa aagttggaga catacgaggt aaaagtaagc ctattattgt tgaacataaa
      481 acaggtaagc gaaaaccgat ttttatagat aatttgagag aagagatttt attatatact
      541 gaaggtaaag aagaaaatga ttggttattt ccaagtcggc aacaaggtcg acatataaca
      601 agggatcgtg tctatcagat atatgcagat atagcagaaa aattaggaag agatgatata
      661 ggaacacata cattaagaaa aacttttggg tatcactact acaagaaaac tagagacatt
      721 gctaccttaa tgtttatttt taatcatagc tcgcaagcaa ttacaaaaag gtacattggt
      781 ataactgaag atgaaatagg ggcatcgtta cgtggtttta aattgggagg ctaattgaga
      841 tattaggata tattttttgt gtataatgaa aacttagaag gtgagattaa tgaagataac
      901 tgaattttcg gaagtattta aaacgtttag taacatttac aatagaatac cagagataga
      961 acaaaatgtt gtagttgata atgattatta tatagagaat gattttaata aaccaagaaa
     1021 atatgacaac ctatacaatt ttatacacaa tagaacaaca ataaataaca acagtaacac
     1081 tcgagttttg attaattcag catattttca tattaaatat ttaaagaaat atgagtatga
     1141 agaagttgta ttagattatg ggctaaatag acagtcattt ttaatttatg caataaataa
     1201 cggaaatttg ccaagcaata aaaagaacat aattattgaa acatatgcct atctggaggg
     1261 gggatctaaa aaactaatta agtcatgtaa tatagagtat caattatcag taggagaaat
     1321 taaatgtgtt ttcgaagaag atttagaact tgacttaaaa aaaatttttg ccagcattca
     1381 tgattatata ggtttga
//
LOCUS       DACEKH010000211         1395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEKH010000211 DACEKH010000000
VERSION     DACEKH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.223"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(201..1184)
                     /locus_tag="IW662_002749"
     CDS             complement(201..1184)
                     /locus_tag="IW662_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ0867531.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          391 a          300 c          265 g          439 t
ORIGIN      
        1 ttttttgaaa ttgctccttg tgactcagag ttaaacggca ggctcacaac ctctgatata
       61 aacggtgttc aatcaactga ccctcggtat tagctcaaaa aacgacaaaa tatgaagagc
      121 tatttttgtt tggctgttct tattacttga tacaggacag ctttttcacg acctctaatt
      181 cacggtgttc aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaag
      241 gtctctaaga tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag
      301 tcatttgctc ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg
      361 gaacgcggga tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg
      421 atcagttctt tttctgtatg ttccctcggg tctttttcaa atgcataagg attcttgaaa
      481 atccctcggc caatcatgat gccatcaacg ccatatttct cggccaattc ttctcctgtc
      541 tggcgatcgg ggatatctcc attgatcgtg attagtgttt gtggagcgat ttggtcacgt
      601 aaagcaatga ttttaggaat gacttcccaa tgtgcatcta ctttactcat ctctttacgc
      661 gtacgcaaat ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct
      721 tccatctcag acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca
      781 gctttagctg cttcaatcaa ttgtgccgct acttctggac gaaggatcaa gccactgcct
      841 tttccacgtt ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag
      901 cccatttctg ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc tccccaaata
      961 tgggcaacca tcggctgttc atcttctgta aatgttaatc ttcctcgtac actgtctttt
     1021 ccatctggat gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct
     1081 gctgcatgct ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa
     1141 acaaaaaaag gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc
     1201 attctatcaa gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac
     1261 aaaaaaagaa gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaaagactg
     1321 tgacaaaaat cttatcacag tccattttcc ggaataaatg gtgttcaaaa gcttgcattt
     1381 gcggtaataa gtcca
//
LOCUS       DACEKH010000212         1374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.224, whole genome shotgun sequence.
ACCESSION   DACEKH010000212 DACEKH010000000
VERSION     DACEKH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.224"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            33..1372
                     /locus_tag="IW662_002750"
     CDS             join(33..474,474..1372)
                     /locus_tag="IW662_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ0867532.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DACEKH010000213         1368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.225, whole genome shotgun sequence.
ACCESSION   DACEKH010000213 DACEKH010000000
VERSION     DACEKH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.225"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            33..1334
                     /locus_tag="IW662_002751"
     CDS             33..1334
                     /locus_tag="IW662_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0867533.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          445 a          275 c          252 g          396 t
ORIGIN      
        1 atcaccacaa cacaatcaaa ggagcgttct gtatgcctga tcttcatcat atcaaactac
       61 tacttggaat caaagataaa aacattttca tcacaaatgt ggaatcgaag agcattaaga
      121 atatcaagag tcttgttgtt tctgcgacgt taagtaaaga aattcgtcgc tgtcctttgt
      181 gtaaacagat gaatcatgaa ggcatgatcg ttaagaacgg aaagaaaaaa tcgctcattc
      241 aacttaataa atgtgcgaac caactgactt atcttgcttt agcgaaacaa cgctaccact
      301 gtcgtggctg ccacacctat tttacggcga atacctacat tgtcgataga aactgcttta
      361 tcgctaaaca agtgcgctat aaaattttag aagagctaac tgaaaaacaa gcaatgacga
      421 caattgctaa gcattgcggc gtttcttggt cgaccgtttc tcggacactc gcttctctga
      481 tccccatgac aaaagtaaaa agaaattggt taccgcgttg tttattaatg gatgaatttc
      541 gttcactgaa aaatcaagtc ggtccctact ccttttcttg tatggatggc gatactggaa
      601 aattactgga tattcttcct tcacggaaga aaaaggctct cgtttcttat tttatgcaat
      661 ttgaacgacg cgcacgattg aaggtaaagg tcatcgtgac ggacatgaat gtttcttatg
      721 cctctctgat aaaggaatgt tttccaaagg caaaactcgt tgtggatcgt tttcacattg
      781 tcaaacatct cattcgtaaa tttgaggata ttcgcgtacg aatcatgaaa agctttgatc
      841 gaaacgatcc aacacaagcc aaatattatc gacaattaaa ggcgttgtct cggcttctga
      901 tcaaacgaca ggacaagctt gtttatgaca aatggaccaa atggcgcaat tttggttggg
      961 cgtatctcac cgaaagtgaa gtggtggatc gtctgctttc gatctctgac gaattacgaa
     1021 tcgcctattt ttactatcaa gaaattcttc aagcgtttca cgacaaagag gcggacactt
     1081 tctttaaact tgtaagaacg atgcccaatt ccgttccaaa agaactgcat tctataaaaa
     1141 aagcttttat aaactatgaa tccggcattc gtttagccct cgaattgcct tattcaaatg
     1201 ccaaaataga aaatctgcat acccacatca aagcattgaa acgagtcgct tatggctttc
     1261 gaagctttcg gaaaatgaag accagaattt tcttactaaa taatctgatt acctatgaat
     1321 caaaaaacat ctaaagtaga aaaggaattc taccttagat gttcttat
//
LOCUS       DACEKH010000214         1366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.226, whole genome shotgun sequence.
ACCESSION   DACEKH010000214 DACEKH010000000
VERSION     DACEKH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.226"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..>1366
                     /locus_tag="IW662_002752"
     CDS             82..>1366
                     /locus_tag="IW662_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ0867534.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          466 a          263 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatca
//
LOCUS       DACEKH010000215         1316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.227, whole genome shotgun sequence.
ACCESSION   DACEKH010000215 DACEKH010000000
VERSION     DACEKH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.227"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            17..1312
                     /locus_tag="IW662_002753"
     CDS             17..1312
                     /locus_tag="IW662_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0867535.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          412 a          262 c          254 g          388 t
ORIGIN      
        1 cataggagga ttcaagatgt ctcatactca gcttatcaaa gataccctaa atattctaga
       61 cctaaatatc tatttcgagg aaaattgttt aactaaagaa aaatacaaag ggcaaatttg
      121 cttgatttac aaaggaactc ttctgtacaa gccagaagag tgttcccatt gtctttgtgt
      181 cgtcccctct aggatcattc gttgggggac gacaaccgtt cggttgttac tgaatgatgt
      241 gtctgagtat cgcacttatc tggagctgaa gaaacaacga tttaaatgta agtcttgcca
      301 aagaacattc gtagcggata cctctgttgc tgaaaaacac tgtttcatta gtcaaaaagt
      361 tcgttggtct gtcattgctc gcttaaaaga gaatacttcc atgacggaaa tcgctcgtca
      421 gaaaaatatt tccacttctt cggtctatcg tgtcatgaag cgattctacc gcccattgaa
      481 tcctttcaaa cagactttgc caaaggtctt atgctttgat gaattcaaat cggttcgaag
      541 tgttgccagt gcaatgagtt ttatcatgat ggacggacaa agccatgcat tattggatat
      601 tgtcgaaaat cgacgcttgc cttatttaga acgctatttt tctcggtttt cattagcgac
      661 tcgtgaagcc gttcaattga ttgttattga tatgtatgcg ccttatgttt cgttggtgaa
      721 aaagcttttc ccgaatgctc agttgatcat cgatcgtttc cacatcgtcc aacatattgg
      781 cagaactttc ttgaatcatc gagtgaaaga aaccacggta cgacttaaag gaaattccca
      841 ctctcaacga gggttaggga aaaaattgaa gcgctattgg aaactcctac aaaaagaaga
      901 agcaaagtta aactatgaaa aacgggtttg gcgtgccagt tttaaagatt atttgactga
      961 aagtgagatt gccgatcgac tgttagctgc ttgtcccaac ttacgccaag gctatcagtt
     1021 ataccaagac attctttatg cggtaaagaa aagagatcag tcactatttg aggattgttt
     1081 aactagagac attagcggtc tgccagaaac ctatacaacc actttacgaa cgtttaaaaa
     1141 gtttctgcca cagatccaaa atgcgcttca ctacagctat tcaaacggac cattggaatg
     1201 cttaaacaac cacatcaaag tattaaaacg caatgcgtac ggctttcgca acttttacaa
     1261 tttcaaacta cgaattatga ttcgtcatgg caaaacattt ttaaccaaat aaacag
//
LOCUS       DACEKH010000216         1300 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.228, whole genome shotgun sequence.
ACCESSION   DACEKH010000216 DACEKH010000000
VERSION     DACEKH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.228"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(62..280)
                     /locus_tag="IW662_002754"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IW662_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IW662_002755"
     CDS             complement(546..740)
                     /locus_tag="IW662_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867536.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          460 a          217 c          177 g          446 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt
//
LOCUS       DACEKH010000217         1285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.229, whole genome shotgun sequence.
ACCESSION   DACEKH010000217 DACEKH010000000
VERSION     DACEKH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.229"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..156
                     /locus_tag="IW662_002756"
     CDS             <1..156
                     /locus_tag="IW662_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAQ0867537.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>1285
                     /locus_tag="IW662_002757"
     CDS             175..>1285
                     /locus_tag="IW662_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0867538.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDS"
BASE COUNT          539 a          107 c          220 g          419 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaa
//
LOCUS       DACEKH010000218         1276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.230, whole genome shotgun sequence.
ACCESSION   DACEKH010000218 DACEKH010000000
VERSION     DACEKH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.230"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            35..532
                     /locus_tag="IW662_002758"
     CDS             35..532
                     /locus_tag="IW662_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0867539.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="IW662_002759"
     CDS             complement(569..1252)
                     /locus_tag="IW662_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867540.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DACEKH010000219         1260 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.231, whole genome shotgun sequence.
ACCESSION   DACEKH010000219 DACEKH010000000
VERSION     DACEKH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.231"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1260
                     /locus_tag="IW662_002760"
     rRNA            <1..>1260
                     /locus_tag="IW662_002760"
                     /product="23S ribosomal RNA"
BASE COUNT          334 a          284 c          380 g          262 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
//
LOCUS       DACEKH010000220         1229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.232, whole genome shotgun sequence.
ACCESSION   DACEKH010000220 DACEKH010000000
VERSION     DACEKH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.232"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(573..>1229)
                     /locus_tag="IW662_002761"
     CDS             complement(573..>1229)
                     /locus_tag="IW662_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0867541.1"
                     /translation="FKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAE
                     VIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQER
                     IDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISEL
                     EKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV
                     "
BASE COUNT          406 a          206 c          167 g          450 t
ORIGIN      
        1 atattaaatt tttatctttt attaaagtca gataaaaatt ctttggaaat gtaatggtag
       61 agatattttt acaaaaggac ttgcagcgct gtagttggtg ctgcaggagg acctatttcg
      121 gccgttacag gaggtattgg taatgtactt gtcggatgtg cattaaagcc tgttcctgca
      181 agttaaattt ataaaaacag gaaagtaatt atctcaaatc ctatcaagta caatcatagg
      241 attgcacttg acaggacaaa ttttttaact aaaaaatgaa aaacactttc taaaaattag
      301 tagaatttgc caaatctttc gcatcttcca ccatacattt aattttaatt cccctttatg
      361 gtacttaata aaaacaagaa aagaactatt catttggccc ttttttggtt ccagaccata
      421 gaaatattcc tccagtaact aatattgctg ttatgcacgt aactaataac agctataaaa
      481 attggtacaa ctttaatagg attcagaaaa atttgcacta gtgaaggaat ctattttatt
      541 agaaaaatgc aatctcccta aagaaaaaac cactaaacag tgctttcttt caacttctcc
      601 ttaatttttt gtgctttccc taaagtaaag tttctggcca actctgaatc aaaatatagt
      661 gaacgttttt taaaatctat ttctttaacg ttaaagatat ttactaaaca cgctcgacta
      721 attcttgtta acataggata tttcttttca agctcactaa ttcttccata aaattcgtac
      781 tgcccatctt tagtacacaa tgataatcta tgtggtaatt ttgaagcttc tacaaaataa
      841 atgtcattta tatcaaaagt aaatgtttga gaacctatgg aaaaaacaaa tgcttgattc
      901 gtaacctgtt ttgttgcatc tatcctttct tgcgctaata acaataactc aacaatctcg
      961 tttcgatact catctagtgt ttgatctttt gttacaaatc ctaacgtttc aactcttctt
     1021 tttattgtta cgggtaacat ctcatcatga gtagttgtaa aaatgatttt tgcttgaaca
     1081 tcatattttc tgattacttc tgctaattct ataccgttaa cttcatgatt taaatcaata
     1141 tctaagaaat atattccttg ctttggtcgg aattgtttga ggtatttttt aacttctaac
     1201 ggactttgtg ttttcaatac tatcttaaa
//
LOCUS       DACEKH010000221         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.233, whole genome shotgun sequence.
ACCESSION   DACEKH010000221 DACEKH010000000
VERSION     DACEKH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.233"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            414..947
                     /locus_tag="IW662_002762"
     CDS             414..947
                     /locus_tag="IW662_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ0867542.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACEKH010000222         1204 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.234, whole genome shotgun sequence.
ACCESSION   DACEKH010000222 DACEKH010000000
VERSION     DACEKH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.234"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(34..405)
                     /locus_tag="IW662_002763"
     CDS             complement(34..405)
                     /locus_tag="IW662_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867543.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(407..808)
                     /locus_tag="IW662_002764"
     CDS             complement(407..808)
                     /locus_tag="IW662_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867544.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(822..>1204)
                     /locus_tag="IW662_002765"
     CDS             complement(822..>1204)
                     /locus_tag="IW662_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867545.1"
                     /translation="SEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSEI
                     EDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAV
                     VKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          381 a          218 c          154 g          451 t
ORIGIN      
        1 aaaaaaataa caaacaaact cgcttgaaat tcattaggtg agtttgttta ttattttttc
       61 tgcttctcga ttcatcagcg ccatatctgt tttcgtaatg gtttcattag gaatatagtc
      121 agcacatttt cttttctcta acattctata cccactatta tgtattccta aggtattcaa
      181 ttccttaact gttgaaaaca aattccaaac ggtttggtgg cttccttttc cgctacttcc
      241 attataaata atatcataat tttctttcaa atactcatca gattttttaa aagcactgta
      301 gtatgctctt gaaacagcag ttcttctata agattcttta gaattatatt tatcatctga
      361 ttcaatttgt ttggaaatag ataaatattc attaaagtca aacattttat tcccccaaag
      421 ttgctactac agatttcccc atatatttat agtcagataa tacattatcg agagtatcca
      481 aaacatcgta atctgaggaa aacaattcag atgttgtttt gataacaaag aaatacaagt
      541 ttaaatcttc atcatatttt atattgattt cagcttttct ttttaaaacc gtatttatca
      601 catcaaaaat tttgccatca ttacttgaaa ttgtgttaat ctcatttttt gtagtaatta
      661 caagttgatt atcattttta gttttcggag tagaacttgc atccacaaca actggagatg
      721 ctagaattac cgatgcgcaa gcaaaaatcg cagcaaaatt tattaagcta ccactccgat
      781 gtgtatttga tggtactata tcaagcatcc gtatcgtctc cttaattttt ttccactggt
      841 ttgttgacct tgatatcaaa gatttgcctt ccaatctcag ttccattaaa tttaacaaca
      901 gcataatgct ctcctgcttc ttcaaccaag acatttctgg cgtttatatt aaagtttata
      961 tcgttaactt gaggaggcaa gcttaccgat acttcacccg tagaaaaaat ttgtttttca
     1021 ttcttgttta aaacttcaat ttctactatt tttatctttt ctgtatcctc tatctcagac
     1081 accacagctg ttacagaaaa tgataaagca gtaggaatta aaggcgcatt aatagaaaga
     1141 gctggattaa ttactttatt aataacatta gtgccaaatt gctgactttc tacatcttct
     1201 gaaa
//
LOCUS       DACEKH010000223         1198 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.235, whole genome shotgun sequence.
ACCESSION   DACEKH010000223 DACEKH010000000
VERSION     DACEKH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.235"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            94..504
                     /locus_tag="IW662_002766"
     CDS             94..504
                     /locus_tag="IW662_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0867546.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            518..874
                     /locus_tag="IW662_002767"
     CDS             518..874
                     /locus_tag="IW662_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ0867547.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          422 a          168 c          224 g          384 t
ORIGIN      
        1 tattttgatt ttttttatat tttattagct aataattggt attttaaaag aaattactgt
       61 agaattggat tataattaaa aaggagtgag aaaatgataa aagtatatac ttcgcctagc
      121 tgtacttcct gtagaaaagc aagagcatgg ttgcaagcga atcaacttga atttgaagag
      181 aagaatattt ttgccgaacc gttaacagaa agtgagatca aaaagatctt ggcagcgact
      241 gaaggaggag ttcaagatat tatttccact cggtcgaaaa tttacgaaaa gctgggcata
      301 gattttaacg aattaacttt aaagcaaatg atcgtcttat tcaaagaata tccttcatta
      361 ttgagacgac ctattttgat tgatgaaaaa agaatactaa tcggatttaa tgaagatgag
      421 atccggtcat ttgtccctcg tgaaattcga tgtgttgcca gaaatctgat ctatttacaa
      481 aacaataaca tcttaaattc atagggaggg agccttcgtg gaagtgaaag tctatggatc
      541 agcgcactgt gtaacaagtc tgaaggcgaa acaatggtta gaacaccatc aattgcctta
      601 tcaatttgtt gacttagaag aacgcgatct aagtacagaa gaagcagaag aattatgcag
      661 ttttgaagaa gtgcaagcag aacagctatt tgcgacttgg tctgaggctt ttcacaacat
      721 tgcgattgac ctttcctgtg taggaaaaaa acaactgatt tttttatgca cacaacatac
      781 caatctcctc agacgtcctt tgattattat cgatgaagtt ctatttattg gatacaatga
      841 gcagttgatg gaacagctta tcttaaataa atagacttta actgttttaa tcaagtcact
      901 gagcatttca tacagttgta acagtcgaac acttagcatc aagcgtgtta tgtgttcttt
      961 ttttgacttt atttttctac tatagagaca tgctcaattt atgtggtcaa aattcaaatc
     1021 taggtgggga tatagagtta aaaatatatt gacatgtgta aatttacatg atatattcat
     1081 ttctgttgtt ttaagtagca aagcaataaa atagtatttt aaaaaacata taaaatagtt
     1141 gttgacatgt tagaaatgac atgataagat aactgagttg ttaaaaaaga accacttg
//
LOCUS       DACEKH010000224         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.236, whole genome shotgun sequence.
ACCESSION   DACEKH010000224 DACEKH010000000
VERSION     DACEKH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.236"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1076
                     /locus_tag="IW662_002768"
     CDS             <1..1076
                     /locus_tag="IW662_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0867548.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACEKH010000225         1173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.237, whole genome shotgun sequence.
ACCESSION   DACEKH010000225 DACEKH010000000
VERSION     DACEKH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.237"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..757
                     /locus_tag="IW662_002769"
     CDS             <1..757
                     /locus_tag="IW662_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0867549.1"
                     /translation="IIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEE
                     KEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELY
                     ANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPM
                     DRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDE
                     IEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            829..>1173
                     /locus_tag="IW662_002770"
     CDS             829..>1173
                     /locus_tag="IW662_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867550.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          517 a          134 c          192 g          330 t
ORIGIN      
        1 aatcataaaa atatttgata aaattaataa tttattcaag aatgacaatg ctgaattaag
       61 gaagggtctt ttagagttta aacttcaaga actagaactt cattactctt atttagtgaa
      121 aattaccgaa gaaaaagaac aacaaaaagc aataaaagaa cagatgatag aagaggaaaa
      181 agttcgtaga gaaattgatc gtgaaaagaa acgtattgat aaagaacaac gtcaattcaa
      241 ttctgaaatt tccaaattaa tcgcatatat gcaaaaagct aatgcagatg tggaaaaaga
      301 attatatgca aataaaattc aagaactgga agaaaaatta aaagaattag aagttatacg
      361 tgaaaatgta ttacaaagag aattaaatac tagagccggt tatgtgtatg taattagtaa
      421 tattgggtct tttggtgaag acatatataa aattggtatg actagaagat tagaacctat
      481 ggatagaata aaagaattga gtagtgcatc ggttccattt gaatttgacg ttcatgcgat
      541 gattttttca gaggacgctc cgtctttgga aactaaattg cataatcatt ttagaaaaca
      601 agaagttaat aaaattaatc aaagaaaaga atttttcaaa gtttcactag acgaaataga
      661 gaaagttgtt ttagaaaatt ataatggaac agtaacattt acaaaattag ctaaagctga
      721 acaatataga cgatctttag aattgtcaaa agattaaata tataaaaagc cttcgggctt
      781 tttcttttaa caattaatag gaacgtatat tcgaaaggaa tagaaataat gtggttagaa
      841 aaaataaatg acaaaaaatt taagtacacc gaacgttata cggatccctt aactgaaaaa
      901 aagagaaaag tgtccgttac tttatctagc aattcacgcc aagcttggaa tcaagctaac
      961 ttacttttga atgaaaaaat cgcagaaaaa ataaaacgaa atgaagaatt accactaact
     1021 tttggagaat taaaaactaa atgggatgaa aaatacaagc caactgtaaa agaaagttcc
     1081 tatcgaacaa ctcaggttta tctatcatta atttcaaaat atataaaaga tgatgtactt
     1141 gtaaaaaacg ttaatagtaa tcttatacaa gat
//
LOCUS       DACEKH010000226         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.238, whole genome shotgun sequence.
ACCESSION   DACEKH010000226 DACEKH010000000
VERSION     DACEKH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.238"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>1035)
                     /locus_tag="IW662_002771"
     rRNA            complement(<1..>1035)
                     /locus_tag="IW662_002771"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACEKH010000227         1034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.239, whole genome shotgun sequence.
ACCESSION   DACEKH010000227 DACEKH010000000
VERSION     DACEKH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.239"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..132)
                     /locus_tag="IW662_002772"
     CDS             complement(<1..132)
                     /locus_tag="IW662_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0867551.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..>1034
                     /locus_tag="IW662_002773"
     CDS             542..>1034
                     /locus_tag="IW662_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HAQ0867552.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSG"
BASE COUNT          417 a          174 c          208 g          235 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaacgga
      541 catggcagaa gaaaaagaac cactagacaa tagtcgcttg ggaaaatcca aaagaaaatt
      601 agtacgattg caaaatgaat taaacgaaca aattgagaag atgtttgaac accagagaaa
      661 gaccaacggt cagccaatga acgataagag aaatgggcat tcttggttta ggcaacaaga
      721 acgtttagaa aacaaagtgc atagtctgag agaagagatc aagcaacaag aaaaacaggt
      781 agaaaaactt gaaagacaag aagagctaaa ggaaatgggc tataacaagt acggcggcct
      841 ggatatgaca atcgaaaata ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg
      901 tgaatcgact ttttcagccg ctacgatccg aaaataccaa cggaaattgg aaacattaga
      961 acagctgaaa gaaagatcag aaaaaggaaa agaaaatctt cttcctgaag tacaagcaat
     1021 tattgacagc ggac
//
LOCUS       DACEKH010000228          963 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.240, whole genome shotgun sequence.
ACCESSION   DACEKH010000228 DACEKH010000000
VERSION     DACEKH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.240"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(13..86)
                     /locus_tag="IW662_002774"
     tRNA            complement(13..86)
                     /locus_tag="IW662_002774"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Ile,seq:cat)
     gene            complement(641..955)
                     /locus_tag="IW662_002775"
     CDS             complement(641..955)
                     /locus_tag="IW662_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867553.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          354 a          150 c          152 g          307 t
ORIGIN      
        1 tggctacatt aatgtaccct gtaggactcg aacctacgac cggacggtta tgagccgtct
       61 gctctgacca actgagctaa gggtagtggt tgttgccaca taaagccata aacaatcaac
      121 cagtagaatg cgtggcaaca aacctgttat cgcatatctt ggagtgtgac tatttatgag
      181 tgatagtgaa gatatgcgtc tccatatatg tatggttgag acagataaac atgtatgaaa
      241 aacgttgtta aatcaacgtt tttttatttt ggtcaaggag agaaaagtag agaaatacaa
      301 aattttttgc attgattttg cattcaaaaa aacattcaaa catttagaaa tccccaatac
      361 gtattcagga aaatctattt taaggtttac aaaaagaact tttgttcgta cactcttttt
      421 gaggtgaact tttatgctga tggaaggaaa aacacaatta tggtttaagc atgttccttc
      481 aaataagttt tttaattata aactctagag aaattaattc atcctaacat aaaataattg
      541 ttcactcaaa agtttttcta gaacaattaa aacagcaggc ttcacattcg ctatacattt
      601 gtaagaagta ataattcctg tactaaaagg cataattgct ttaattattt tttgacttct
      661 cttcaaacac aagaagtagt tgtgtaataa ttgcagaaat aaggattcca ataatagata
      721 taaaatttga catggaaacg tctataacat taaaagaata aagtgttgaa gaaagtaaac
      781 tgattgaaat aacccctccg taaaaaatag tctgtgaata tacccacttt ttttcagaag
      841 acgtacttcg tttggttcta aaacctatcc ctgcatttct actcttatca aaaaaccaaa
      901 tatttagaag taataatatt gctattatta agataaaatt actgtaaaaa tccaaaagct
      961 atc
//
LOCUS       DACEKH010000229          956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.241, whole genome shotgun sequence.
ACCESSION   DACEKH010000229 DACEKH010000000
VERSION     DACEKH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.241"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(28..357)
                     /locus_tag="IW662_002776"
     CDS             complement(28..357)
                     /locus_tag="IW662_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867554.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
BASE COUNT          284 a          212 c          125 g          335 t
ORIGIN      
        1 gtatgtgtgc ccaatcgtat attcggatca taatttttca tttgttgtaa cctcctcata
       61 atcagttagt actataaagg tatctttgtt tttatccaaa aaatctaata aatcttttgt
      121 agaagatatc tcttcatcaa cttcaccata ataaatacct tcgataattg ttgctatatc
      181 tgaaaaacta atttttactg gacatttatc gtctaccgca ttcaataccg acaaaattgc
      241 ttctttttct gcaatctctc gtttgaattt ctttggagct aatcgcatga cactcaagtc
      301 attttcaagc ttttcaatcc atgcagtttt actcattttc gcaaattttt gaatcattcc
      361 tctaccacct ttgctatcta ctaaattagt tttacttcat aacgaatatc tttatcgttt
      421 ttcgttcact caaaaatata taaaataaat cgactcgttt atggcgtccg aaacgagaaa
      481 ccctagtaga cagcggtaaa tgctcgattg atccaatgat caacgtcctc gcaagtatct
      541 acatgtactg cagcacacga cacaatttct tgtgcgtcct ccccattacg agaagaccta
      601 ctccttattc catgagtttg cgtcaaagcc tagatccctt agtagctgct catgattaat
      661 ctaggcgcta ttctaacttg tctaacttct aaattccctc ttgtttactc tgtgcatatt
      721 atttacacaa attcatttca ccactatcca aacaagggca tttactcgat tgaccaattt
      781 tttacgttac caatcgtaaa aacctacccc gtcactcccc gaagggaatt gttgatagat
      841 caagtctact ccttattttt ctagtgattc taagttcctt tagaagtctc gacaatattt
      901 tcaagcaagc gaaaaacgat aaaatacaag ttatgagaat gatcaattca gctgat
//
LOCUS       DACEKH010000230          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.242, whole genome shotgun sequence.
ACCESSION   DACEKH010000230 DACEKH010000000
VERSION     DACEKH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.242"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..>943
                     /locus_tag="IW662_002777"
     rRNA            2..>943
                     /locus_tag="IW662_002777"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACEKH010000231          940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.243, whole genome shotgun sequence.
ACCESSION   DACEKH010000231 DACEKH010000000
VERSION     DACEKH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.243"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            94..>940
                     /locus_tag="IW662_002778"
     CDS             94..>940
                     /locus_tag="IW662_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0867555.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE
                     LARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGA
                     QDFHDK"
BASE COUNT          320 a          163 c          197 g          260 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac
//
LOCUS       DACEKH010000232          823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.246, whole genome shotgun sequence.
ACCESSION   DACEKH010000232 DACEKH010000000
VERSION     DACEKH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.246"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..>823
                     /locus_tag="IW662_002779"
     CDS             22..>823
                     /locus_tag="IW662_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0867556.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT          253 a          142 c          186 g          242 t
ORIGIN      
        1 cgaggagggt ggaaagaaac cttgccagaa attgcggatg aaatcagtat cctcaacttg
       61 acgagtaagt cagaaaatca aggtctgctt gatccttatg tgattatgaa gaaaaagaaa
      121 gactcggaaa gtttagcaat tgatattctg acttttttaa caggaatttc aagtcgagac
      181 ggggagaagt ttcctgtgtt acgtaaagcc attcgaacgg tgactcaaag tgatcaacgg
      241 gggttattac aagttattaa cgaattgcga aaagacgggt caccaatcgc ggaaaatatc
      301 gccgatcata tcgagtcctt taccgattat gattttgctc atttgctttt ctcagacggc
      361 tcgattaccc aatcgattag tttagataaa cagctaaata tcattcaagt ggcggatttg
      421 gttttaccgg atgcagaaac gacttttgaa gaatatacca ccatggaatt gcttagtgtt
      481 tcgatgctaa tcgtcatttc cacttttgcc ttggacttta tccatagtaa ccgagaaatc
      541 ttcaaaattg tcgatttgga tgaggcttgg agttttcttc aagtggctca aggaaaaacg
      601 ttatcgaata agcttgttcg tgctggtcgg gccatgaatg ctggggtcta tttcgtcact
      661 caaaatagcg acgatttatt agatgaacgt ttaaaaaata atatcgggtt gaagtttgct
      721 tttcgcagta ccgatattca tgaaatcaag aaaaccttgg aattctttgg tttggatcag
      781 gaggatcaaa gcaatcaaaa acgcctgcga gatttggaaa acg
//
LOCUS       DACEKH010000233          803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.248, whole genome shotgun sequence.
ACCESSION   DACEKH010000233 DACEKH010000000
VERSION     DACEKH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.248"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            380..778
                     /gene="spxA"
                     /locus_tag="IW662_002780"
     CDS             380..778
                     /gene="spxA"
                     /locus_tag="IW662_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ0867557.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          304 a          109 c          141 g          249 t
ORIGIN      
        1 aaataagcgt tataataaag aacagtatta tttaaaatga cagaaaacat tactattaaa
       61 gaaataaaaa acataaaaaa tcttaagaaa tcggtttcat ttagcttttg agataaaata
      121 atagtacagt tttatacgct gtttcatatg tttagaaaat ttgaattaga tttgaaaact
      181 ctaattttta aatgataaaa aaagcgagaa tgctggtttt actgtatatt aaccttagat
      241 aactttcatt tttttaaaaa aattatgaaa aaaaggtagc tatttaaaag ttgattatgt
      301 ataatagaaa cagatgggag attattatga tcttctgtta caaaaaagtg attcttatca
      361 cgaaaaggag tggtgcacgt tgttaacatt atatacttcc ccaagttgta cttcctgcag
      421 aaaggctcgt gcgtggttgc aagaacatca gattccattt gttgaacgta atattttttc
      481 agaaccattg aacagttctg aattaaaagc gattttacaa atgacagaag acggaacaga
      541 agaaatcatc tcgacacgtt ccaaagtatt ccaaaaactg aacatggatt tagatgatct
      601 gccgttacaa gaattattgg aacttgtgca aaacaatcct ggattgcttc gccgtccaat
      661 catgattgat gataaacgtc tgcaagttgg ttttaatgaa gatgaaattc gtcggttcct
      721 accgcgcgat gtacgacaat tagaattacg tcaagcacaa ttgatggctg gattatagaa
      781 ggataatacg aaacttggaa aag
//
LOCUS       DACEKH010000234          793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.250, whole genome shotgun sequence.
ACCESSION   DACEKH010000234 DACEKH010000000
VERSION     DACEKH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.250"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..782
                     /locus_tag="IW662_002781"
     CDS             <1..782
                     /locus_tag="IW662_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0867558.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          244 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc ttt
//
LOCUS       DACEKH010000235          790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.251, whole genome shotgun sequence.
ACCESSION   DACEKH010000235 DACEKH010000000
VERSION     DACEKH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.251"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            24..716
                     /locus_tag="IW662_002782"
     CDS             24..716
                     /locus_tag="IW662_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0867559.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          198 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt
//
LOCUS       DACEKH010000236          788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.252, whole genome shotgun sequence.
ACCESSION   DACEKH010000236 DACEKH010000000
VERSION     DACEKH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.252"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            553..>788
                     /locus_tag="IW662_002783"
     CDS             553..>788
                     /locus_tag="IW662_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0867560.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRIGTLEL"
BASE COUNT          285 a          145 c          124 g          234 t
ORIGIN      
        1 acactcctct taatatacag tcctattgta taaagtgtta ctatatacat agtaactgta
       61 tacataaaaa aagtcaagta ttagaaaaaa gttcaaaaga tcaagtgtgc gcttcgctcc
      121 cactcccctc gacgttttag tagcctttcc ctcacttcgt tcagtccaag ccaactaaaa
      181 agttttcggg atacattctc cttctcttcc taataatcaa ttaaaatttt aatctttata
      241 tttctgctaa tcactcagga aacagtacag aaaaacaaac acgtatcata gatataaatg
      301 taatggcata gtgcgggttt tattttcagc ctgtatcata gctaaacaaa tcgagttgtg
      361 tgtccgtttt tgggcgttct gctagcttgt ttaaagtctc ttgaatgaat gtatgttcta
      421 agtcaaaaaa ctctgacagc gcctttatat agataaagtc cgtataattg tgtaaaagta
      481 aaaaggccat ataacagtcc ttttacggta caatgttttt aacgacaaaa acatacccag
      541 gaggactttt acatgaccca agtacatttt acactgaaaa gcgaagagat tcaaagcatt
      601 attgaatatt ctgtaaagga tgacgtttct aaaaatattt taacaacggt atttaatcaa
      661 ctaatggaaa atcaacgaac agaatatatt caagcaaaag aatatgaacg aacagaaaac
      721 cgacaaagtc aacgaaatgg ctattatgag cgcagcttta cgacacgtat aggcacgcta
      781 gaattaaa
//
LOCUS       DACEKH010000237          751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.254, whole genome shotgun sequence.
ACCESSION   DACEKH010000237 DACEKH010000000
VERSION     DACEKH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.254"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            362..730
                     /locus_tag="IW662_002784"
     CDS             362..730
                     /locus_tag="IW662_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0867561.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT          243 a          117 c          157 g          234 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt caaaaaatag
       61 cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga gaacaccgtt
      121 taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt ccatataaat
      181 acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa cttatttatt
      241 attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta gattttccca
      301 ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta ggaggaaaca
      361 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
      421 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      481 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      541 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      601 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      661 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      721 aggtaaataa tagaaataaa gactttgttt t
//
LOCUS       DACEKH010000238          690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.256, whole genome shotgun sequence.
ACCESSION   DACEKH010000238 DACEKH010000000
VERSION     DACEKH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.256"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            312..608
                     /locus_tag="IW662_002785"
     CDS             312..608
                     /locus_tag="IW662_002785"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0867562.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLSTVCMKKLDVC"
     gene            complement(531..690)
                     /locus_tag="IW662_002786"
                     /pseudo
     CDS             complement(531..690)
                     /locus_tag="IW662_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          230 a          131 c          102 g          227 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttccacggt ctgtatgaaa aagcttgacg
      601 tctgttaaag aatacggtat ccgtccaaag gcttctttta ccaatgaggc atctttcttc
      661 tcaccacagg aataaccaat aatttctcga
//
LOCUS       DACEKH010000239          671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.258, whole genome shotgun sequence.
ACCESSION   DACEKH010000239 DACEKH010000000
VERSION     DACEKH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.258"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW662_002787"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW662_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ0867563.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..>671)
                     /locus_tag="IW662_002788"
     CDS             complement(481..>671)
                     /locus_tag="IW662_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867564.1"
                     /translation="KLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNG
                     INNYILQAILKELLSNAL"
BASE COUNT          202 a          145 c          111 g          213 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta t
//
LOCUS       DACEKH010000240          670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.259, whole genome shotgun sequence.
ACCESSION   DACEKH010000240 DACEKH010000000
VERSION     DACEKH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.259"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(188..490)
                     /locus_tag="IW662_002789"
     CDS             complement(188..490)
                     /locus_tag="IW662_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867565.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          238 a          107 c           91 g          234 t
ORIGIN      
        1 ataatgggaa aaagacttat aattggagtg gtagttaatt agtgacttat ttttgatttt
       61 atagcactga tactataaaa tatagatatc atcatattac acaatcttaa tactaactta
      121 aaaaatatcc cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta
      181 gcctacttta atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac
      241 gccgatgatc gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat
      301 ctttcttcat tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga
      361 gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat
      421 aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt
      481 ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta
      541 ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct
      601 attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac
      661 ccttataaaa
//
LOCUS       DACEKH010000241          620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.260, whole genome shotgun sequence.
ACCESSION   DACEKH010000241 DACEKH010000000
VERSION     DACEKH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.260"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            51..>620
                     /locus_tag="IW662_002790"
     CDS             51..>620
                     /locus_tag="IW662_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0867566.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIIT"
BASE COUNT          214 a          116 c          116 g          174 t
ORIGIN      
        1 tgctacactc aaaatagttc cgaccaaaga attataagga gtgagtacaa atgacctata
       61 cccatcttac aacggatgaa cttgtaataa tagagtctta tttcaaaatg aatcaatctg
      121 ttgctaaaac tgcccattgc ttgaatcgtt caagacaaac gatccataaa gtatatctgt
      181 tcttcaagca aggaaaatca gccttagaat attatcaaca gtataagaaa aacaaatcaa
      241 actgtggtag acgtccgctt gttttacccg aggaacaatc agaatatatt caaagaaagg
      301 ttgttcaagg atggacaccc gatgtgattg ttggtcgtgc agcgtttcct attagttgtt
      361 ctgctcgtac catttatcgt atgttcaaaa aggggctatt tgattcttct gacttaccga
      421 tgaaaggcaa gcgtaaaccg aatggacatc aagaaagacg tggaaaacaa actttccgcc
      481 gctctattca tgaacgtgaa aaggattata gccaattctc aaatgagttt ggtcaccttg
      541 aaggtgacac tatcgtaggt ctgaaacata aaagtgctgt aattacctta gttgaacgat
      601 tatcaaaagt tatcatcaca
//
LOCUS       DACEKH010000242          560 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.262, whole genome shotgun sequence.
ACCESSION   DACEKH010000242 DACEKH010000000
VERSION     DACEKH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.262"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(38..>560)
                     /locus_tag="IW662_002791"
     CDS             complement(38..>560)
                     /locus_tag="IW662_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0867567.1"
                     /translation="RRSKHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRL
                     LYYDDSLKRGYEVYQVFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEI
                     KRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLT
                     KARTTTRLDKIAV"
BASE COUNT          142 a          110 c          122 g          186 t
ORIGIN      
        1 gaagaaattc aatagaataa tcgagatact cacacggcta gacagctatc ttgtctagcc
       61 gtgttgttgt tctggcttta gtcagttggg tagtgttctt agttttcttg aaatattttc
      121 ctcgatataa aaagattcgt tctcggtagt tttgaaagtt tctaaagcca taggcaattc
      181 gttttagtac tttgatatgg ttgttcaaac attctatagg gccgttggaa tagggaactc
      241 gcaacgctcg tttgatttct gttcgatatt tcttgaaggt cgtgaacact ggttgatagt
      301 gctctggcaa ctccttgtag tcttctttta ggagcgcgac gaattcagga acatcttgtc
      361 ttctgatagc ggaaagaaaa acctggtaga cttcgtagcc tcgctttaag gagtcatcat
      421 aatataatag ccgatcgact atctccgctt ctgtcagatg atctcgaaat gacggacgcc
      481 agtagcgatg ctgaccattc aattcccacg cattcttttg aagtagtctc cagtatttct
      541 ttaagtgctt cgaccgacga
//
LOCUS       DACEKH010000243          555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.263, whole genome shotgun sequence.
ACCESSION   DACEKH010000243 DACEKH010000000
VERSION     DACEKH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.263"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(271..534)
                     /locus_tag="IW662_002792"
     CDS             complement(271..534)
                     /locus_tag="IW662_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAQ0867568.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          215 a           97 c           75 g          168 t
ORIGIN      
        1 actctcctga attttttcat gttgaaaaca tcactaatca aaggcaaatt ataatttaga
       61 aatagaatga aaacactaaa tgaaaataga acattagaaa aaatagaatc aaaaaaaccc
      121 aagactagtg ccgaaataag ggaaacaatc gaagctacaa aaaataatga tttcgttgaa
      181 caggaccaat ttatttgttt ccgatatttc tcaaaaagaa ctttctttgg gaaagttaaa
      241 ggcgactgta aagtcgcctt tagtcctttt ttaccaagct gctgcatatt tagctccata
      301 ttttttaatt aatgatttgg ctgtagcaac aataccagct gtaattaatc cgcctccaac
      361 aactgcagca acaatagaaa tgattgtagc aaccgtgcta ccagtattaa ttatgtcaat
      421 aactttttta gctgcataag aagaaattcc aatgttagca cttagcattg gtacaccaac
      481 aaagaatccc gcaaaaccta aaattggtaa aactaataat aaattctttt tcataataat
      541 tcctctattt tttta
//
LOCUS       DACEKH010000244          553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.264, whole genome shotgun sequence.
ACCESSION   DACEKH010000244 DACEKH010000000
VERSION     DACEKH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.264"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..523
                     /locus_tag="IW662_002793"
     CDS             <1..523
                     /locus_tag="IW662_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ0867569.1"
                     /translation="YGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADG
                     LTLDIWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTE
                     HRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFS
                     VCTEIKVLLGIPA"
BASE COUNT          199 a           98 c          106 g          150 t
ORIGIN      
        1 atatggcaaa ctactctatc aaatttggaa aaagaaaaat aaaaaatcct tttattcatg
       61 gaaaatggat gaaacgtaca tcaaaattaa aggaaaatgg cattatttgt atcgagccat
      121 cgatgcagat ggtttaacct tggatatttg gttacgtaaa aaacgggaca cacaagcagc
      181 ctatgctttt cttaagcggt tagtgaagca gtttgatgaa ccgaaggttg tagtcacaga
      241 taaagccccc tctattacaa gtgcctttaa gaaactaaaa gaatacggct tttatcaagg
      301 gacagaacat cgtaccatta aatacctgaa taatttgatt gaacaagacc atcgtccagt
      361 aaagagacgc aataaattct atcgaagttt acgcactgcc tctaccacga ttaaaggcat
      421 ggaagccatt cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc
      481 ggtctgtact gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag
      541 ggattttatt ctt
//
LOCUS       DACEKH010000245          544 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.265, whole genome shotgun sequence.
ACCESSION   DACEKH010000245 DACEKH010000000
VERSION     DACEKH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.265"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          178 a           75 c           89 g          202 t
ORIGIN      
        1 cactataaaa atgatcaatt ttaaattaga acttttttac aattttaatt ttattacaaa
       61 tgttacaaat aaatagttat tattccggag tttttagatt aaaagtttat gaaaacaatc
      121 acaaacgtag gttttaatgt cataatcgtt agatatttta gttatttttc attcaaaaaa
      181 acagggaact agtgtttgca tatgtatttt tttgagttat aataattcat gaagagaggt
      241 aattcactta tttacccaag ctgaaaagcc tgatattact tcttttcagg cagtgctgct
      301 aatattcaat tacacttttt tgtatatttt tagttgtttt aaatttaatt gtctgatatg
      361 cttgtgatga attgattttc gttctgcact tttggacgat aaattgtgat aattgaaaat
      421 acaaaaaaaa gaaaggcccc tgtaaggctc gggagactgg taatctccgt cagtgtccta
      481 caggaaagca ttaagatgac gccttaatgc gtgcccttca gtgttacggt attacccgca
      541 acgg
//
LOCUS       DACEKH010000246          533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.266, whole genome shotgun sequence.
ACCESSION   DACEKH010000246 DACEKH010000000
VERSION     DACEKH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.266"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>533
                     /locus_tag="IW662_002794"
     CDS             <1..>533
                     /locus_tag="IW662_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0867570.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGEREK
                     QFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEINCVVADAGYKTP
                     KFVHFLTHLNLRPCLPY"
BASE COUNT          179 a          100 c          105 g          149 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 agaaaaacaa tttgcttatt ctgcccatac ttcatgcgac gacaatggat ttattcttag
      361 cacaatcatc actcctggaa atattcatga cagtcaagtc gcctttcagc tcgtcaaaca
      421 atcgaagaga ctttttcctg aaatcaattg tgttgtcgct gatgctgggt ataagacacc
      481 taagtttgtt cactttttga ctcatttgaa ccttcgacct tgtttgcctt att
//
LOCUS       DACEKH010000247          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.denovo.267, whole genome shotgun sequence.
ACCESSION   DACEKH010000247 DACEKH010000000
VERSION     DACEKH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.denovo.267"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..90)
                     /locus_tag="IW662_002795"
     tRNA            complement(5..90)
                     /locus_tag="IW662_002795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IW662_002796"
     tRNA            complement(107..178)
                     /locus_tag="IW662_002796"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IW662_002797"
     tRNA            complement(190..262)
                     /locus_tag="IW662_002797"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IW662_002798"
     tRNA            complement(280..361)
                     /locus_tag="IW662_002798"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IW662_002799"
     tRNA            complement(369..441)
                     /locus_tag="IW662_002799"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IW662_002800"
     tRNA            complement(445..517)
                     /locus_tag="IW662_002800"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACEKH010000248        23334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.guided.035, whole genome shotgun sequence.
ACCESSION   DACEKH010000248 DACEKH010000000
VERSION     DACEKH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.guided.035"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..225
                     /locus_tag="IW662_002801"
     CDS             4..225
                     /locus_tag="IW662_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867571.1"
                     /translation="MPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGL
                     LWFIPVALCAAEMATVKGWEKGVSQTKRY"
     gene            620..1093
                     /gene="dfrF"
                     /locus_tag="IW662_002802"
     CDS             620..1093
                     /gene="dfrF"
                     /locus_tag="IW662_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0867572.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            1220..1876
                     /locus_tag="IW662_002803"
     CDS             1220..1876
                     /locus_tag="IW662_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0867573.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            2058..2588
                     /locus_tag="IW662_002804"
     CDS             2058..2588
                     /locus_tag="IW662_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0867574.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            3134..3376
                     /locus_tag="IW662_002805"
     CDS             3134..3376
                     /locus_tag="IW662_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ0867575.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            3373..3843
                     /locus_tag="IW662_002806"
     CDS             3373..3843
                     /locus_tag="IW662_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0867576.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            3971..5185
                     /locus_tag="IW662_002807"
     CDS             3971..5185
                     /locus_tag="IW662_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0867577.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(5895..6083)
                     /locus_tag="IW662_002808"
     CDS             complement(5895..6083)
                     /locus_tag="IW662_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867578.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            6174..6383
                     /locus_tag="IW662_002809"
     CDS             6174..6383
                     /locus_tag="IW662_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ0867579.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            6495..8108
                     /locus_tag="IW662_002810"
     CDS             6495..8108
                     /locus_tag="IW662_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ0867580.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            8143..9354
                     /locus_tag="IW662_002811"
                     /pseudo
     CDS             8143..9354
                     /locus_tag="IW662_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            9377..10303
                     /gene="glsA"
                     /locus_tag="IW662_002812"
     CDS             9377..10303
                     /gene="glsA"
                     /locus_tag="IW662_002812"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0867581.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            10511..11971
                     /locus_tag="IW662_002813"
     CDS             10511..11971
                     /locus_tag="IW662_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867582.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            12192..13955
                     /locus_tag="IW662_002814"
     CDS             12192..13955
                     /locus_tag="IW662_002814"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0867583.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            14039..14497
                     /locus_tag="IW662_002815"
     CDS             14039..14497
                     /locus_tag="IW662_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0867584.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            14606..15289
                     /locus_tag="IW662_002816"
     CDS             14606..15289
                     /locus_tag="IW662_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0867585.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            15436..16050
                     /locus_tag="IW662_002817"
     CDS             15436..16050
                     /locus_tag="IW662_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0867586.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            16066..16272
                     /locus_tag="IW662_002818"
     CDS             16066..16272
                     /locus_tag="IW662_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867587.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            16813..17943
                     /locus_tag="IW662_002819"
     CDS             16813..17943
                     /locus_tag="IW662_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0867588.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            18035..18790
                     /locus_tag="IW662_002820"
     CDS             18035..18790
                     /locus_tag="IW662_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0867589.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            18848..20887
                     /locus_tag="IW662_002821"
     CDS             18848..20887
                     /locus_tag="IW662_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0867590.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(20996..22147)
                     /locus_tag="IW662_002822"
     CDS             complement(20996..22147)
                     /locus_tag="IW662_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0867591.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(22301..22831)
                     /locus_tag="IW662_002823"
     CDS             complement(22301..22831)
                     /locus_tag="IW662_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0867592.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(22930..23280)
                     /locus_tag="IW662_002824"
     CDS             complement(22930..23280)
                     /locus_tag="IW662_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0867593.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         8123 a         3771 c         4751 g         6689 t
ORIGIN      
        1 aaaattcctg caaaaaaatt aacgttcttt ggctttttgg ctatgaccat ctcaatggtc
       61 gtttcattat atgaatatcc cacattcgcg acctcaggct tcagcttagt atttttcttg
      121 ttattaggcg gtttgctatg gtttatccct gtggcattgt gcgcagcaga gatggcaacg
      181 gtgaaaggat gggaaaaagg tgtttcccaa acaaaaagat attaagaaac agcccatgtt
      241 tacctgcttg caaacaaaag aatattgcta ctagactaag agatactaca taagtgtgtt
      301 ggtatctctt ttttgttgcc gtgaaacagc agactatttc atttctgctt gattacctta
      361 aaacttatta gtctatggtt tgtcaagggc ttgttgcgtg agaaatgctg aatatcaccc
      421 tttacaaaac ataggctgat aagtaacttt tcaaaagcag aaaagaaatg gcaatgctta
      481 taacaatatg atataatgca gatgaagaca aaaaaggaat ttgtcgagct ggttagttcg
      541 agataatttt gagtttgtta agatgtggtg cacttgatgg aatcacaggt acgacatcat
      601 attatggagg atgaaataga tgataggttt gattgttgcg aggtcaaaga ataatgttat
      661 aggcaagaat ggtaatatac catggaaaat aaagggagaa caaaagcaat ttagagagtt
      721 aacaacgggt aatgtggtta ttatggggcg aaagtcttat gaagaaatcg gtcatccgtt
      781 gcctaataga atgaatattg ttgtttccac cacaacagag tatcaaggag ataatttagt
      841 ttcagttaaa tcattagaag atgcattatt attggctaaa ggacgagatg tatacatatc
      901 tggtggatat ggactattta aggaagcttt gcaaatagta gataaaatgt atatcacaga
      961 agtagattta aatattgaag atggagatac attctttcca gaatttgata tcaatgattt
     1021 tgaagttttg ataggggaaa cacttggtga ggaagtgaaa tatacgagaa cattttatgt
     1081 aaggaaaaaa tgaattgagt aaattttgga tttagggtgt tttcataaat atattgcttt
     1141 ttatgcatat ataaattgtt gtaagacaaa ttccagtttg tatggggcga taaaatcaga
     1201 atttgagaga ggattgaata tgaataagga atggtctgaa cttaataaaa caatgcaggc
     1261 acaaataaaa aagaaagata cttataagag gggtattgat actttactta ctttacgaag
     1321 tcagttaata caaaccttag tatctttcaa agaagaatta tgcagagaag attttaactc
     1381 aatccccttc ataaacgctg atggttatca tagtaagacg attgcttatt ctatttggca
     1441 tattttccgc attgaagata tagttgtgca tacagtgata aatgaagatg aacaagtatt
     1501 ttttgcaggc aattatcaag agcgtatcaa ttcacctatc attacaacag gaaatgaact
     1561 catgaaacag cagattgcag acttttcaaa acagcttaat ctggaagaat tatatttata
     1621 tattttcgaa gtatgggaaa gcactgagaa aatgcttgaa cggttatctt atgatgaatt
     1681 gaaaagaaaa ataccgaaag aaagaaaagg atacttagaa tcgttgaatg tagtaaacga
     1741 caatgaaaaa gcaatttggc tgattgatta ttggtgtaat aaagatattt gtggactaat
     1801 tcaaatgccg ttttcaagac attggattat gcacacagaa gcctgtttgc gtataaagaa
     1861 caagatacat tcataggcaa aaatttcagt ttgtagaact aaggaaattg gaatttaagg
     1921 aggaataaag tatggttagt gattttataa gagcagcatt tccatggatt ttaatgggtt
     1981 tatttgtagc aatcagttgt tcccttatga gtaaaaaaga gaaataaatt cctgtttgta
     2041 tttataaaag agaaaaaatg agaaaagtag ttttatttat tgccatgagc cttgacggat
     2101 atattgcgga cggtaatggt ggagtagctt ggctaaatgg tcatggaaat gacaatgaaa
     2161 acatcgacac ttatacagaa tttaccaaag atatagatac tgtcttaatg gggtggaata
     2221 cttctcatca ggttgttact gaactttctc cgcaagaatg ggtatataat aaatttacaa
     2281 cttatgtatt aacacataaa gagtgcaatt cttcccaaca aattcatttt acaagtgaaa
     2341 atcctgtttt attattggaa cggctaaaac aagaagcagg aaaggatatt tggatttgtg
     2401 gcggagcaaa tcttgttcag cagttggtaa gcaaaaatat gattgataaa tattacattt
     2461 ctgttattcc tactctgtta ggaaatgggg ttcgtctttt gggcaacata gacagggaaa
     2521 taaagttaag gctttgtaaa acacagactt ataacggaat aaccgatttg atttatatgc
     2581 gtagataact agagagaaac agaaaagctg ttaaaccgac ggagcgatcc gaccacgttg
     2641 gcgggaaaat ccttaagatt tttccgccat gcttgcaagg gggcggttcg cttcggcggt
     2701 tcggcgagct tgcgagccgt tcaaagcccc cgttatctaa caaggggggc acaaaaaaca
     2761 agaaacacta tacactaaca agcggaaaac gctgtattta taaggcaaaa ccccattttt
     2821 acagtagtga tataaattgt acctatatca ctatgaaaat tgaatagtaa atatgagaaa
     2881 gacagttgat ttcaaaaaat aagaaattga ttgtcgtttt cttttgcaga aatttaagtg
     2941 caggcaatta tggtagttgt cgtaattgct gtcagctcag tgtttatcac tgatggtgtg
     3001 aaagatttac ggttggagat gttcaataag attattggtg cttaattatt tttattgtag
     3061 tgctgatatg agaatgttat aattggggta taaaacatac aaataaattt gctgaaaaat
     3121 gaggtatcaa aatatggaaa atgaattact ggaaaactta ggaagacttc acacaactga
     3181 acttggcgtt attagaatta aaaagaactt atctttgaat gtagaaaatg taattgaatg
     3241 gtgcaaagaa aaaattagcc tttctaatgc ggaaatcaca agaaaaggaa aaaactggta
     3301 tataacgatt gataattgta ttataacggt aaatgcgtac agttataccg ttatcactgc
     3361 tcacaaggta aaatgataaa tttttctata aaggaattaa acgcatttgc agttattggg
     3421 caggaagtag aactgactaa ttatcaaaaa aagaatattc agattagtac acagttttgg
     3481 agaaagttta acagtagttt gaaaaaagcg tatctttcac aatcaggaaa ttgggtaaaa
     3541 tatgctttta tggaaagaag aaatggaaaa ctttattatt tctgttctat tccccaaaga
     3601 accattaccc cagagggctt tctgtataaa gaaataccgt cttataaata tttggttgtg
     3661 gagcatattg gtgctatgga taaaatatat gaaacttatg gcaagattta tgaagaaatt
     3721 attcctagta catcgtatat tcctataaaa gatattatcc tacactttga aaaatacgat
     3781 tatcgttttc attggaatag tgataattca gttattgaaa tttggatacc tattcaagat
     3841 tagaagcaat tttatatcca tacacacaaa gcagttagtc ttaggattga ctgctttttc
     3901 tatacagatt tttaatgaca ggcaattatc aattatggta gttgcctttt ttgattggag
     3961 gaattgaaga atgaatgata aaaactttat tgaagaacta agacaaaagc gtgaggaata
     4021 cggagtaaca caaacaaggc ttgctgttgc ctgtggtatc agccgtgaat attacaaccg
     4081 cattgaaaaa ggaaaacagc cattgaatga cgaattaaga gaaatcatag aaaaacagat
     4141 tgagcgtttc aatccgcaag aaccactatt cttattgatt gattactttc gtgtccgctt
     4201 tcctactacg gacgcattgg cgattatccg tgatgtatta caactgaaat ctaattacat
     4261 gctttatgaa gattatggaa aatacggata tgaaagcaaa tatgtacttg gcgacatcaa
     4321 tatcatgtgt tccatgcagg agcatttagg tgttttattg gaactgaaag gcaaaggctg
     4381 tcggcaaatg gaatgttatc tgctggcaca ggaacgctca tggtatgact tcatgctgga
     4441 ttgtatgacg gctggtggtg tgatgaaacg gcttgatttg gctatcaatg ataaagcagg
     4501 aatattggat attccaaagt taaaggaaaa atacaaggct ggcgaatgta tgtcctactt
     4561 ccgtatgcag aaagattaca gcggtacaga aaaatgcggt agcgatttac caaagaatac
     4621 aggagaaacc ttatatctcg gttcaacaag tagcgaatta tatatgtgtg cttatcagaa
     4681 aaattatgag cagtatgtca agaatggcac agaaattgaa gatacggaga ttaagaaccg
     4741 ttttgaaata cgcatgaaga acgaacgagc ctattatgcg gttgtagatt tactgaccta
     4801 tcgggacgct gaacgcaccg ctttttctat catcaatcat tatgtccgct ttgttgacag
     4861 agaggacgac aagccaaaaa gtcaatggat aacaaatgat gattgggcat ggtttgtagg
     4921 ggaaaacaga gagccgatac gcttaaccac aaagccagag ccttacactt tacaaaaagc
     4981 gttgcattgg ctacaaagac aagtcgcacc aaccataaaa atggtacagg cattagacag
     5041 agaaaatcgt acaacgatac tgaaagatat gattgagcag gcagaactaa aagacaagca
     5101 taaacattta ttacaattag agaaatcaac catagaagaa cgtatagata ccgctgttcc
     5161 acaagagaat gacggtattt tttaatgtaa ttgaaaaatt tcagatttag tcagcaaaga
     5221 gctgttttca ttccctatgt gaagtgaaag aagaaaaata aattttttat taaatctgca
     5281 acaaaatggc tacttgcgta cagatatggt gcgagaaaag gaaatctaaa ctttttttga
     5341 aaataggtca ttaaatcggg gcttgctgtc cctatatgaa gtgaaagaag aaaaaagttt
     5401 tgaaaaaatg acggaaattc gtgattagtg gacagtcttc tgtgaaaaga ggaaaaacat
     5461 catcattttg tattcaaaca ggcattagca gttgagtgtt gatgtgaaaa gatgaaaata
     5521 ctttgttctc aacttaacaa tttaaggttt ccgttcccta tatggtgcaa agaaaatatt
     5581 tcattctatt gttggcagta acggtatggt ttgggtagtt agggtgtgaa aaggaaaata
     5641 ttcttttttc atcatcaact tagcaaaatg acattttcta tccctatatg gtgcatgaaa
     5701 agaagttaaa atttttccat ttaaacttag caaatggacg tttgctgtac agatagtagt
     5761 gaaaaaggag aaaatcaacc gttgattgcc aaaatgaacc gttccatttg tactaataat
     5821 ggaaaaagtt ttggattttg gttacgtttc tcctcttttc caacgcgata tataggaaag
     5881 acgatttttt tctttcaatc gttctttgac aactgaataa agctattcaa tacgtttgac
     5941 agacagctag ccgttgaaac aaatacgcca cgacctttcc cctgcaagag cgagcgaaaa
     6001 cctatttgtg ttcttgtaaa agatttgtcc tcttttatcg ccatgacctg tactgtcaga
     6061 taatgatact cccgtacagc cacagcttga gcattaagag cgtcgcacgc aatgggtacg
     6121 gctacataag aactatgcag aggtggaact cctgtgggct atgacaaaag ccgttgaatt
     6181 gcttagaaag attttacaga aagggggtat gtgttattga taatgctgta aaaacaattc
     6241 aaaagaaaaa tggcaagtgt ggcattggca acagaggaaa gacaaagatt gtagtaaaag
     6301 agcatttttc cgaaaagggc aaaacaatgg aagaacttct aactgatgtc atgctggaaa
     6361 aagcaaagca gacaatcgca taaaatcggt gcagttgtaa gaaatagatt gaatgacaaa
     6421 aagtacccgt gttatacttt acttgcaagc aggtattgta aacacgggtt agttataaag
     6481 gaggataact gacaatgaaa caacagattt acaatactgc actttatctt aggttaagcc
     6541 gagatgatga attacagggc gaaagttcca gcattaccac acagagaagt atgttgcgtt
     6601 tatatgcaaa agaacatcat ttgaatgtca ttgatgaata tattgatgac ggctggtcgg
     6661 gaacaaattt tgacagaccg agttttcaaa gaatgattga ggatatagag gcaggaaaaa
     6721 tcaactgtgt tgtaacgaaa gacctttccc gtcttggcag aaattatatt atgacaggac
     6781 aatatacaga attgtatttc cctagtcata atgtccgcta catagcgatt gatgacggtg
     6841 tagacagcga aaaaggcgaa agtgagattg caccatttaa gaacatcatc aatgaatggg
     6901 tggcaagaga tacaagccgt aaagtcaaat cagcatttaa gacaaagttt gcggaggggg
     6961 ctcattacgg tgcttatgct ccgttaggat ataagaaaca ccctgacatc aaaggaaaac
     7021 tgttggttga tgatgaaaca aaatggatta ttgaaaaaat cttttcccta gcctatcaag
     7081 gttacggaag tgcaaaaatc acaaagcagt taagagcaga aaaagttccg acagcgtcat
     7141 ggctaaattt tacaagatac ggtacttttg cacatatctt tgagggaaaa cccgaaagta
     7201 agcgttatga gtggacgatt gctcatgtca aggcgatatt gaaaagtgag gtttatatcg
     7261 gaaacagtgt tcacaatatg cagtctacgg tatcgttcaa gagcaaaaag aaagtgcgta
     7321 agcccgaaag tgaatggttt cgagtagaaa acactcacga gccgattatt gacaaggaag
     7381 tgttctatcg tgtgcaggag cagataaaat caagacgcag acagacaaag gaaaaggcaa
     7441 cgccgatttt tgcagggctt gtcaagtgtg cggattgtgg ttggtctatg agatttggaa
     7501 cgaataagac aaataaaacg ccttacagtt attatgcttg cagttactac gggcagtttg
     7561 gcaaaggtaa ttgttctatg cactacattc gttatgatgt gctgtatcaa gccgtattgg
     7621 aacgcttgca gtattgggct aaggcagtac agcaggacga agaaaaggta ttgaacaaga
     7681 tacagaaagc tggcaatgca gagagaatac gggaaaagaa gaaaaaggca agtacattga
     7741 agaaagccga gaacagacaa aatgagattg accgtttatt tgcgaaaatg tatgaagata
     7801 gagcctgtga gaagataaca gagcgaaatt ttgtgatgtt gtccagcaag taccaaaaag
     7861 aacagataga actggaacag cagataacaa gcctaagaga agaactaagt aaaatggaac
     7921 aggatatgat aggtgctgaa aagtggattg agttaatcaa ggaatattcc gtaccaaagg
     7981 aactgacagc accattatta aatgccatga tagaaaaaat cctcatacat gaagcaacaa
     8041 cgaatgagga taacgaaaga atacaggaaa ttgagatata ttaccgattt attggaagag
     8101 ttgagtaatc aacaagagta atatttttaa ctaagggaaa ccggcggtgt ctatacttgg
     8161 gtcagtcgca cattgggaaa acgattcggt tttgccgcaa tctttttcca atggtttgag
     8221 ataacagttg gctacttgac catgctgtat ttcttaactg gtgcattatc ctatgcaacg
     8281 ggtattggtg ctatacaaaa caataaatat ctgaaattgg caatcctatt gattattttc
     8341 tgggctattt tactctcaca gttaaaagga accaaataca cttcattgat tgctcggatc
     8401 ggttttgtag ctggtatctt gttgccagca ttagtcttgt ttggcttagg gatccattat
     8461 gtcgcttcag gcgctccact acaaacgata ctgtctatga aaacattgat tcctgatttt
     8521 tcgaaattgc ctacgctggt tgtttttgtt tcctttattt tagcatatat gggcgtagaa
     8581 acatctgcca gtcatgcaaa tgaaatggaa aaccctaaaa aggattatcc attagcaatg
     8641 tttgtattgg ttattctggc aatcatttta gatacttttg gcggattgac cgtcgctact
     8701 acagtacctc aacatggttt gtctcttaat acaggggtga ttcaatcgtt agaattccta
     8761 ttaagacaca ttgatccaaa tcttgcatgg attgcgaaaa ttcttgcgat tcttgtttgt
     8821 ttaggtgtca tcggagaaat tgcttcttgg gtaacctctc cttcaaaagc acttcacgta
     8881 gcagcagaag aaggattact tcctgaatat tttgcaaaag aaaacacaca cggtgttcct
     8941 gctcatttga tgatcgcaaa tgggatcgtt gcaacaatct gggcagccgt ctttacatta
     9001 agcggcgggg ggaataatct ttcatttctt gcagcgatgt cattgactgt tgtaatctac
     9061 ctgatgttgt actttatgtt tttcgcgggc tatttgaagc tggtttttaa agatgacagt
     9121 cttgaaagag cctatcaggt tccaggcgga aaagttgtaa aagtaatcgt tgcttgttta
     9181 ggtcttttga cttcactttt tgctttttgt atctcatttg ttccacctgc tagtatcgca
     9241 gctggacagc atgggattta tgaaacgatt ctgattgttt cttatcttat cgtcttagtt
     9301 attccattta cgatctacgc atgccgtcat aaattccagc atgcagaaaa ataaaaaatg
     9361 aaagctggtt attcaaatgg aagtaaaaga attagaacaa ttgattggaa aaaatttcca
     9421 ctattatcaa acaggacaag tggcggatta cattccagca ttagctgaag tggatcccaa
     9481 acaattaggt atggcgattt atgatttgga tacaaaacaa ctgattgagg gaggagacgc
     9541 cgatgtgcgc tttgccatcg aaagcatgtc aaaagttccc gtattgttat tagcaatcaa
     9601 agacaacgga atagaaaaag tctttcaaac aatcaacacg gaaccaaccg gttttgcctt
     9661 caattctatc atgaatatgg aaatcaatca tcgcaaacat ccaatgaatc cttttgttaa
     9721 tgcaggagcg attgcaacaa cctcgttgat tgccggtaaa gatgcagatg aaaaatttga
     9781 gcgaatcttg tcatttatga aagagatctg tgatgatcct aatatcacat tgaatgaaga
     9841 aatctatcat tctgaatcaa gaacaggtga tatcaatcgc tctcttgctt actatatgaa
     9901 aggtaatcaa atgatcgaag gagacgtacc ggagatactc gatgcttatt tcagacaatg
     9961 ctccctcaat gtcaccgcta caggcatagc aaaattagct gctgtgctgg caaataaagg
    10021 aattgctcct tggaatggca aacaaatcat tactgaagaa agtgctacaa tcgtcaaatc
    10081 aattatgaca actgctggtt tgtatgatga atcaggagag ttttccgtcc atgttggtgt
    10141 acctgcaaaa agcggtgtag gcggcgggtt gatggcagct gtgccaaatc gttacggcat
    10201 aggtgtcttc agcccggcac tagatccatt tggtaacagc gcagctggta tccaattgtt
    10261 gaaagatgta gtcaaagaac tagacgctga catctttgaa taaccattct tgaaagaggc
    10321 tggggtaaaa gtccaaacgg ctcaaagcta cctacaatga ggctgaaatt gtgagcttgt
    10381 ttgacttttg tcccagtttc tttttttatt ggtttttgaa agggcttttc taaataaaaa
    10441 ggaaacaaaa atacgattta cagatatgat taaataaaat cataagaaaa actggaggcg
    10501 tgtatcagga atgctggaaa attacatcga gcgaaatata tttagaaaag tgtatctgtg
    10561 tgaacaatta tttgaattcc aagaaataga catcgaacaa acagctattt ctctaagggt
    10621 gactacacca acgatccttc acgatctaga aagtctagcc gagtgtctag aatattgtat
    10681 aaaagaacaa gtaagagaga agcataagta taaactcgta ttcaaacatg gtattgctct
    10741 ttctgagctc acacagtttc tgtacggtca atcttatttt ttaaagtttt taagttatta
    10801 ttttaatggt atattcacga gtacagaact ggctgatctt gaatttattt ctttgagtaa
    10861 agtatataca atcaaaaaaa tcgtattgga tttttttaaa gaaaatgatt atttgaaaaa
    10921 taaagaaatc attatcccag aatttgactc gcggaacatc ttgctagctt tagtacgtta
    10981 tgtcaattgg gaaggctatg aaaataaaaa tccgtatgta gaagaagctt gtaacgagtt
    11041 gattgattat gtagagactc atttttttaa aagaagatat tcggaagatg aaaagtcctt
    11101 tatcatacga gaaatagaga tcgcaatcgg aagaaaggca tttccccttg gactgaaaga
    11161 agaagataag aaaagcgcag aaacgaagcc gttatttcaa ttagtctgtc aaggattgga
    11221 agagcaaaaa ggaattctag acttacaaga agaagatgtc tattatattt tttctttgtt
    11281 caacagtaga aattatacca atgagaacat ggagctgtta agaaaagatt tcgaagttgt
    11341 ttataaaaat tttgttctca atgacctatc actgaatgaa ttgattttac agattattag
    11401 tatcgctgaa atcgacgatg cagaaaattt tattttcaag caatcctttt tacaatttgt
    11461 ccgtacgttg tgggcagatg gacaagtatt tttaccggaa aggatctatc ttctatcaga
    11521 aaaagaacaa cttctgtacg gaaaaattgt agaaatattg gaagagtgga aaaagaaaaa
    11581 tcaaatccaa ctaagatgga atgaaaattt cattcgaaaa ttcgtcaaaa gtctttcttt
    11641 gatcaacagt gaacaagcgg ggagacagga aatagaaatt tttgttgttt cagaaagttc
    11701 agtcaaacaa tttttttatc gaacccaatt tcatttgtat gcggaagaag gcatcgctga
    11761 aataaatcct attttatttc gttcattaga agaattgcct gatgaatgtc tatgtgcgaa
    11821 aaaaagagtg gttttgtgcg atgctgctag ctatcaaaat ggattagaaa ctgaaaagac
    11881 aaagatctat ccaatctcat taaaaggtgc aaacatccat ttgtatcata ttgctcagga
    11941 acttcaccta ttaaaaaaac atatttcttg atgagacctt aattaggaga ccttctacaa
    12001 agctttttaa ttgtctgtag aaggttttta tttgagtcct gattcgtgcg aagatctact
    12061 tatataacaa gctttctctg aaattttttt aaaatgctat tgacttagag tcaactctaa
    12121 gtagtagaat agataaagtg taacaaatct attttcatta aaagaagata gtacggagga
    12181 gagaatgtat tatgagtaca gtattacatg tattggatgc aatcaatagc cccgcagatg
    12241 tcaaaaaatt atctgttgag gaaatgaaac agttggcaga agagatgcgc caaaaaatct
    12301 tgcaaaagga cagcgatgta ggtgggcacg ttggtccgaa tctaggtgta gtagaattaa
    12361 cgattgcgtt tcattatgta tttgattcac caaaggataa aatcgtttgg gatgtttccc
    12421 atcagtctta tggtcataag cttttaactg gtcgaaaaaa tggttttgaa aaaggacatt
    12481 accgtgaagt gagcggatat tccaaccaac atgaaagtaa acatgactat tttacagtgg
    12541 ggcacacatc tacatctatc agcttagcaa caggtttagc taaagcgaga gatctgaaga
    12601 aagaatcagg aaatgtggta gcatttatcg gtgacggctc cctaagtggt ggcttagctt
    12661 ttgaaggatt gaacaatggt ggtgttttac aatccaatct aatcgtatta gtaaacgata
    12721 atgagatgtc tattgatgag aatcatggca gtttatataa aaacttagct gagcttcgtc
    12781 aaaccaacgg taaaagcagc aataacctat ttaaagcaat gggatttgat tatcgttacg
    12841 tggataatgg aaacgatctg gaaacggtga tccgtttatt tgaagaagta aaagatattg
    12901 atcatccgat tgtacttcac gtccatacgg aaaaaggtcg cggttatcag ccggcaatcg
    12961 ataataaaat gaaatatcat tggcatgtac catttgatct gtccaccggg gaggcaaagc
    13021 aagtaacttc tggagaaaca tttaatatga tcatcatgga ttatttggat aagaaaatgc
    13081 aaacaagtac agctcctatt gccgcagtca atgcagcgat accaggcgcc tttggactga
    13141 aaacattcca agaaaaacat ccagatcatt attttgacgt ggggatcgct gaacagcact
    13201 ctatcacttt cgcttctgct ttggcttctc agggagtacg tccagtcgtc ttccaatcaa
    13261 gtactttctt gcaacgggca tatgatcaat tatcacatga tttagcgatc aacgagaatc
    13321 cagtgattat tttagtcggt ggcggaacga tctcagctag tgaccgaaca caccaaggaa
    13381 catttgacat tccttactta tcaagtatcc cgaacatcaa atatcttgct ccagcaacaa
    13441 aagaggatat gatctctatg ctagattggg cgttcgaaca aacagcgcag cctgtagcaa
    13501 tccgaattcc tacacatggt gtagaacatg gtacagctat cgcaaatgat tatagtattc
    13561 cagcctatca aacagtaaat aatggaggaa aagtagcaat tttagcttta ggcggttttc
    13621 ttgaattagg aaaagaaaca gtaaaagctt ttagaaaaaa aggaatcgaa gcaacgttga
    13681 tcaatccagg aacgatcaca gaaaatgacg tagaaacatt agataaatta aaagaaacac
    13741 acgaattagt catcacatta gaagatggta gtttgtcagg aggatttggc gaaaatatca
    13801 gtcgttatta tggtgattct tcaatgaacg tattaaattt tggtgcagag aaagaattca
    13861 cggatagcgt tcctgtaggg cagctttatg aacgttatca tttaacacca aaacaaatcg
    13921 ttgaagatag tctgcgtgtc ttgaatattc aataattcat tatgctcacc atccagataa
    13981 ttgtttatac tagtatctgg atggtttttt cattataaaa agcaaagggg tggatgttat
    14041 gtatcagcaa gtagaagaat atgtcaaaaa gttccaagaa aaatatccta gttatcaatc
    14101 agaaaaatta ggtttctttt catttgggga tacagaaaaa atggcagacg agttgagcga
    14161 actagttgta aaaaagataa agactggtac atcaagtggt tacgatctgt ataaacaaga
    14221 gaatgagcct ttaccaaaag ttggtcaatt agacgtggtc ctgaatggaa aaaatgaacc
    14281 agtttgtatc atcaaaaata taaatgtgac catcttgcct tttagtgaag tcacgaaaga
    14341 gcatgcttgg aaagaaggag aaggggatcg gacattagct tattggcgga aagtccatgc
    14401 tgagtttttt aaacatgctt ataaagaagc gttgaacata gaatttacag aagagaagct
    14461 ggtcgtatac gaagaatttg aagtaatttt cttttagtaa gaaaggtctg ctgaaagaaa
    14521 tagctcatta ttgtctcctg aatagaaaag ggttacattt ttatatgaag ctgtattttt
    14581 atataacatt actggaggag atattgtgga tacactggaa caaatcaaag gcggagtcgt
    14641 cgtttcttgt caagctttgg aaaacgagcc gctacacagt tcttttatta tggggagaat
    14701 ggctttggct gcaaaagaag gcggagcagt tggtattcga gcaaatacga cagaagatat
    14761 caatgaaatc aagcgaatcg ttgatttacc ggtaataggg attatcaaaa gagattacga
    14821 tgggtcagaa gtgtttatta ctgcgacaga aaaagaagtg gaagaacttt tggagacgca
    14881 agcagaaatc attgcactgg atgcaacttc tcgtaaacgt ccgacagaaa aaacaactgc
    14941 tgaacttgtc caaatgattc acgaacacgg tcggttagct atggcagata tttcgacttt
    15001 tgaggaagcg cttcaagcgc aagaagatgg ttttgatctt ttgtctacga ctttagcagg
    15061 ctatacagaa tattcacgaa agacgcagac acctgatttt gatttattga atcaaatcat
    15121 agaacaagtc gatatcccag taattatgga aggacatact gactctcctg aacaagtaga
    15181 gaaagcatat gaattaggtg ctttttcagt cgtagttgga tctattatca cccgtccgca
    15241 attgatcact aaaagatatg tcgaagccgc aagtaaagca aataactaaa aaacacgcac
    15301 ggcacagatt cttgacaaaa aagaaaaatt gatagatagt ttaaagcaga ttctaagaag
    15361 atgaaaaaag cattactttt cattttgtct tcaagatctg ctttttaacg aattttatag
    15421 aaagaaggcg aaaccatgtt tgaacggagt ccttcagaat tgacagaacg tgaaaattac
    15481 aagttgctga ttggttcagt catccctaga ccagtagcag tagtaacgac taaatcagca
    15541 aaagatatag tgaatatcgc acccttcagt tacttcaata tcgtcagttc gaatcgaccc
    15601 atcttatcct tggcgatcca gagaaaacaa gatgcagtaa aagacacagc aaagaatatt
    15661 cttgagacaa gagaagctgt tatccatatc cttgatgagg acaacgtaga agaagtaaat
    15721 aaaacagcag ccaatctgcc taatgaggaa agcgaactgt acctgactga cttgacttta
    15781 actgattcag taatcatttc agtaccagca ttgacagaag caaaagttcg ttttgaaaca
    15841 tccttatatg aacatgtcga gatacggaat aaaaataaag taacagctga tttatttttg
    15901 ctagaagtac agaaatatca tttaaatgaa acagtctatg atgataagac aggaaggatc
    15961 gatccaagaa aattgaatgc tgtaagccgt ttggcaggaa atgattatgc agcaatagga
    16021 gaaattttca cgattgcacg tcccgaataa aaaggagcac tagttatgtt aaaaataagc
    16081 tataaaccat cgacagattc aaaagaaatg aaaaaagaat atgaaacagt gaatgatttt
    16141 ctgcaagggc agtacttaga agttccgcca ttgcaagacc attttgtcgt tactactgtt
    16201 actttagatg gtaaggaaat agaaatgcca gatcaaacga ttagcggatt gtttaattac
    16261 tttaacaaat aaatatttat tccgaaaagt gaaaaaacat ataaaaaaac acataaaact
    16321 aaaaaattat caaagaatcc tggtgcaact gaaagaattt gcaacgggat tctttcaact
    16381 tatttgttat ttttttctaa aaaatattaa agattgttaa atttcactat ttcatttcaa
    16441 attccattgc ctaatcatta ggaaaagtct atcatgaact cgtaaataga atatagtaat
    16501 aaccttgtta ggtgaggctc ctatacaaac ataggctgtt gcccagaaac gtcgaaagtc
    16561 gccaatgggt aaaacagaaa ctgttggatt aaggcttttt ccaatagcta agaatgtttt
    16621 ctttacgttg tatagtgcta aaactcggac gatgagatga tgctaggtca gaattcttgt
    16681 tcgaaaaggt ttctgacctt ttctttatat ttttgtgact gacttatttg atgagacaaa
    16741 ctcgctcctc acctgacaag tggattgctg tgtgaggagg agatgcggtg aaaaaagatc
    16801 gattggtgga ttttgggtcc ttatttgact ttgtccatga tcggtctttt agaagtatac
    16861 agtgccagtt cttatcgttt attgcaagcc gatgaaaata caaaaagtct attattgcgt
    16921 caactaattt tcatattttt gagttggagc gtgatcttct tagctcgttc agtcaaacta
    16981 cactatttac ttcaccctaa aatagcagga tacggtttag ccttatcgat tttcttttta
    17041 gtattagtaa gaatagggat attcggtgtc actgtcaacg gcgcacaacg ttggatctct
    17101 ctgtttggca ttcaattcca gccttctgaa ctggcaaatc tttttttgat tttttattta
    17161 agctggtttt ttcgtgacgg aaatagtagc ccaaaagatc taaaaaaacc attcctgatt
    17221 acagtaggta taacttttct gattttattt cagccaaaga ttgctggagc attgatgatc
    17281 ctttcgattg cgtgggtcat attttgggca gcggcggttc catttaaaaa agggatctat
    17341 ctaatcgtta ctttttctgc attgctgatt ggagcagcag gcggggtatt atatttagga
    17401 aataaaggtt ggcttccaca gatgtttaat catgcttatg aaaggatcgc gactttaaga
    17461 gattctttta tcgatagtca tggtgcgggc tatcaaatga cccattcgtt ttatgcacta
    17521 tataatggtg gcatatttgg gcggggatta ggaaatagta tcacaaaaaa agggtatctt
    17581 ccagagacag aaacagattt tattttttcg attattacag aagagcttgg gctaatcggt
    17641 gcattgtgtg tgcttttctt attgttctca ttatgcatga gaatcttttg tttaagcagt
    17701 cgctgtaaaa atcagcaagc tggattgttt cttttaggat tcggaacttt gctattcgtt
    17761 cagacgataa tgaacgtggg aagtatcgcg gggctgatgc caatgactgg tgtgccattg
    17821 ccatttgtta gctacggagg aacaagttat ctgatccttt ctttaggaat cggaatcacg
    17881 ttgaatattt cttcaaagat acaggccgaa gagttggcgc tctatcgacc tgaaaaacaa
    17941 taaagagaaa ctgagaaact ctcagtttga attatcataa agtacaatct tttatttttc
    18001 aagcgagagg ttcttttaga aagaggaatt cgcaatgaaa gtatctcaaa aaatcatatt
    18061 atctattttg ttactgctta cattagtagt tggctatttt tcgattgagt ttatccatgg
    18121 attttcgtct gcaaaacaga gttcaactgt gaaaaaggta gagccgaaaa gtatccctac
    18181 cacactaaat gtggctttga ttggttcaga tgctcgttcg aaagacgaaa acggtcgctc
    18241 ggattcactt ctggttgcac aatacaacca gaaaacacaa caagcaaaac tgatctctat
    18301 catgagagat tcatatgtcg atattccagg ttacggaatg aataagataa atgcagctta
    18361 ctcttatggg ggagttgatt tattaaatca aacattaaag gaaaatttca aatttgaagc
    18421 tccctattat gcaagtatca catttcaaga ttttatcgat tgcgttaatg aactgtttcc
    18481 tggtggagtc aagatcgatg cagaaaaatc tctagattta gatggcgtat atataaacaa
    18541 aggagagcaa gtgatggatg gtaatacttt actccagtat gctcgatttc gggaagacaa
    18601 agaaggagac ttcgggagga tcagaaggca gcagcaagtg atcaaagcta tctctcagca
    18661 gctgaaagac gtaacttcaa tattcaagct acccaaagcg gttgggaaat tacttggtag
    18721 tatccaaacg aaccttccag aaagtgtgct tctggattgt ggtatggatt tcctcaaaga
    18781 catatgttag aataaacagg tataaatagt gaaataaagg aatgacaagc aagagaagga
    18841 ggaaaaaatg aaaagaagtg acaagcacgg caaaaatcga acaggcgctt atattgccgg
    18901 cgcagtgatc ttaatagcag ctgcgggtgg cggttatttt tactaccagc actatcaaga
    18961 aacccaagca gtagaagctg gagaaaagac agttgagcaa tttgtccaag ctttgaacaa
    19021 gggagattat aacaaagctg cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt
    19081 atctgaaaaa gagatcttag ataaatacca gaatatatac ggtgctgctg atgtcaaagg
    19141 acttcagata tcgaatctaa aagtagataa aaaagacgat tctacttaca gtttttcata
    19201 taaagcaaag atgaatacct cattaggtga attgaaagat ctttcttata aaggaacatt
    19261 agacagaaat gatggtcaaa ccacgatcaa ctggcaaccc aacttggttt ttccagaaat
    19321 ggaaggaaat gacaaagtaa gcctgaccac acaagaagca gcaagaggga atattataga
    19381 tcgaaatggt gaaccattag caacaacagg caaactaaaa caattaggag tcgttccaag
    19441 taaacttgga gatggaggcg aaaaaacagc caatatcaaa gcgattgctt cctccttcga
    19501 cttaacagaa gatgctatca atcaggcgat ttcacaaagc tgggtacaac ccgattactt
    19561 tgtcccattg aaaatcattg atggagcaac gccagaactt ccagctggag ctaccattca
    19621 agaagtagac ggcagatatt atcctttggg tgaagcagct gctcagctga ttggttacgt
    19681 gggagatatc acagcagaag atattgataa gaatccagaa ttaagcagta atggaaaaat
    19741 cggtcgctca ggtttggaga tggcttttga taaggatctt cgtgggacaa caggtgggaa
    19801 attaagcatt acagatgcag acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca
    19861 aaatggcaaa gatatcaaat tgacaatcga tgcgaaggca caaaaaacag cttttgacag
    19921 tctaggagga aaagctggat caactgttgc gacaacgcca aaaaccggtg atcttcttgc
    19981 gcttgctagc tctccaagct atgatccaaa caaaatgaca aacgggatct cacaagaaga
    20041 ttacaaagct tatgaagaaa atcctgaaca tccattcatc agccgatttg cgacaggtta
    20101 tgctcctgga tctacgttta aaatgattac agcagcaatc ggtctcgaca acggcactat
    20161 cgatccaaat gaagtgttga cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg
    20221 atcttatcaa gcaacgcgtg tcagtagtga tgtatcacaa gtagacttaa aaactgcttt
    20281 gatctattcc gataatatat atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt
    20341 tcgtacaggc ttagataaat tcatttttgg tgaagacctt gatttgccaa ttagtatgaa
    20401 tccagcacaa atttctaatg aagatagctt taactcagat atcttgctag ctgatactgg
    20461 atatggacag ggcgaacttc taattactcc tatccagcaa gcagcaatgt attctgtttt
    20521 taccaacaat ggcacacttg tctatcctaa attgattgca gataaagaga caaaagataa
    20581 gaagaatgtc atcagcgaaa cagcagtaca aacgatcgtg ccagatctga gagaagttgt
    20641 gcaagatgta aatggtacag cacattctct ttctgcttta gggattccgt tggcagcgaa
    20701 aactggtaca gcggaaatca aagaaaaaca ggatgtaaag gggcaagaga acagtttctt
    20761 gtttgctttc aatcttgata accaaggtta tatgatggtc agcatgttgg aaaataaaga
    20821 agatgatgat tcagcaacca aacgagcatc ggaactattg caatacctca accaaaatta
    20881 tcaataaaaa attgcttttt aagtacagta aaaggactgt ggcgttatcg tcacagtcct
    20941 tttttcaaat aaacggtttt cacgatgtct ctttcacaac ttctttttga tttactcaca
    21001 cttcataaac tttttttgta aaatatttga gaacaagtgg agaaataagt gtacttaata
    21061 aaatcacgat aactaaaggt gaatagtact gtggttcgat caattgactt tgctgtccaa
    21121 tttgtaaaat gatcaaagcc atttctccac gcgaaatcat accagcaccg accatcaatg
    21181 aactatgttg agaaaaaccg gcaatctgag caccaaaata acctccaagc aatttggtca
    21241 aaatcgctac aagcgtcaat atagaaataa agagtagttg ttcactaaag tcagaaaaat
    21301 caacttctaa tcctacactg acaaaaaata ctggtataaa tactgcatat cctaaagcct
    21361 ctacattgtg gtatacttct tctttaacct ttgtctgact gacagctatc ccagcaaaaa
    21421 aagcaccaat gacagagcta agcccaacaa gatccgctac gtaagacatt cctaaacaaa
    21481 taactagcga tacgatgata atagaagaat ttgcgtacac tttttctgca aatgaaagta
    21541 ggaaaggcgc aatccatttg actaataaga aaataaaaac aaaataaatt agctgttcaa
    21601 tcaaaacaag cggtaaagaa gcattgctag aagcttcccc cgttagaaaa gataggctga
    21661 aacttagaac taaaacgact aaaatgtcat ctaccacaga agcacccaaa attgttgatc
    21721 cttcttttgt attcactacg tttagttctt ttaatacttc tactgaaata ctgacagaag
    21781 ttgccgcaag aataatccca aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc
    21841 ctgtcaacca gccaaaaaca accgggaaga tgatcccaag caaagcaacg aacataccag
    21901 gtcggaaata ttttttcaaa agagataaat cactttccag accagctaaa aacattaata
    21961 agatcacacc aatctccgaa aagtcatgga ctagaatatc aggtttgacc caattcaatc
    22021 cagcttggcc tagcaaaaca ccaacaagta attgtccaat cactgctggg atacctaatc
    22081 tccgcgagat atgtgagcca atcgttgttg aaatcaatat caggcataaa ataccaataa
    22141 attccattct catcattcct ttgtataaaa tatagcccga acttagcata acatacatta
    22201 ttgaaaaaat aggaaaatat actcacaaaa aaaccaacaa tccaaaagat ttttttggat
    22261 tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc
    22321 ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata
    22381 tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca
    22441 acaacagaat actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca
    22501 actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga
    22561 ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca
    22621 gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca
    22681 acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag
    22741 ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt
    22801 tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa
    22861 cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct
    22921 ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat
    22981 cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac
    23041 accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa
    23101 aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc
    23161 cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt
    23221 tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat
    23281 aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa gaaa
//
LOCUS       DACEKH010000249        17124 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.guided.061, whole genome shotgun sequence.
ACCESSION   DACEKH010000249 DACEKH010000000
VERSION     DACEKH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.guided.061"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(134..577)
                     /locus_tag="IW662_002825"
     CDS             complement(134..577)
                     /locus_tag="IW662_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0867594.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IW662_002826"
     CDS             complement(795..2135)
                     /locus_tag="IW662_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0867595.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IW662_002827"
     CDS             2404..3300
                     /locus_tag="IW662_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0867596.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IW662_002828"
     CDS             complement(3450..4391)
                     /locus_tag="IW662_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0867597.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IW662_002829"
     CDS             4517..5638
                     /locus_tag="IW662_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0867598.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IW662_002830"
     CDS             5896..7398
                     /locus_tag="IW662_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0867599.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IW662_002831"
     CDS             7465..7992
                     /locus_tag="IW662_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0867600.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="IW662_002832"
     CDS             8203..9264
                     /locus_tag="IW662_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0867601.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="IW662_002833"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="IW662_002833"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0867602.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="IW662_002834"
     CDS             10177..12159
                     /locus_tag="IW662_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0867603.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IW662_002835"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IW662_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0867604.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="IW662_002836"
     CDS             12881..14305
                     /locus_tag="IW662_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0867605.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="IW662_002837"
     CDS             complement(14486..15259)
                     /locus_tag="IW662_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0867606.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="IW662_002838"
     CDS             complement(15256..16161)
                     /locus_tag="IW662_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0867607.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="IW662_002839"
     CDS             complement(16315..16635)
                     /locus_tag="IW662_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0867608.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         5547 a         3106 c         3499 g         4972 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaaaaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat ctta
//
LOCUS       DACEKH010000250        16995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.guided.062, whole genome shotgun sequence.
ACCESSION   DACEKH010000250 DACEKH010000000
VERSION     DACEKH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.guided.062"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            587..1450
                     /locus_tag="IW662_002840"
     CDS             587..1450
                     /locus_tag="IW662_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0867609.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            1550..2032
                     /locus_tag="IW662_002841"
     CDS             1550..2032
                     /locus_tag="IW662_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0867610.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2295..2762)
                     /locus_tag="IW662_002842"
     CDS             complement(2295..2762)
                     /locus_tag="IW662_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0867611.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            2885..3736
                     /locus_tag="IW662_002843"
     CDS             2885..3736
                     /locus_tag="IW662_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0867612.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(3987..5945)
                     /locus_tag="IW662_002844"
     CDS             complement(3987..5945)
                     /locus_tag="IW662_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0867613.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(5942..6814)
                     /locus_tag="IW662_002845"
     CDS             complement(5942..6814)
                     /locus_tag="IW662_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0867614.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(7156..8028)
                     /locus_tag="IW662_002846"
     CDS             complement(7156..8028)
                     /locus_tag="IW662_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0867615.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            8156..8725
                     /locus_tag="IW662_002847"
     CDS             8156..8725
                     /locus_tag="IW662_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0867616.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8792..9529
                     /locus_tag="IW662_002848"
     CDS             8792..9529
                     /locus_tag="IW662_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0867617.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            9760..11028
                     /locus_tag="IW662_002849"
     CDS             9760..11028
                     /locus_tag="IW662_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0867618.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            11515..11979
                     /locus_tag="IW662_002850"
     CDS             11515..11979
                     /locus_tag="IW662_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0867619.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            11963..12592
                     /locus_tag="IW662_002851"
     CDS             11963..12592
                     /locus_tag="IW662_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0867620.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            12946..14424
                     /gene="msr"
                     /locus_tag="IW662_002852"
     CDS             12946..14424
                     /gene="msr"
                     /locus_tag="IW662_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0867621.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            14471..14917
                     /locus_tag="IW662_002853"
     CDS             14471..14917
                     /locus_tag="IW662_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0867622.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15116..15631)
                     /locus_tag="IW662_002854"
     CDS             complement(15116..15631)
                     /locus_tag="IW662_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0867623.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            15748..16949
                     /locus_tag="IW662_002855"
                     /pseudo
     CDS             15748..16949
                     /locus_tag="IW662_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         5383 a         3007 c         3511 g         5094 t
ORIGIN      
        1 ttcatgaaac acttatttat atttagtata aagaaacgtt taagaaaaaa gtagctgatt
       61 ttacatattt gataatcact tttgctcgaa ttgtcaaaaa ttcattctcc ttttcttttc
      121 ttagagaatg tgagaaaaag taaaagaata gcgtgtttta aagatggaac tttttttgtt
      181 ttttatttat ctgacaaaaa tgaagtgatt ttgttagaaa taaataattg ttccaattga
      241 taaaagaggt atgatgtatc gcaaagaaaa atccaagcag cgttctgatc agcagtttga
      301 acaacaattt gctttgtaca gtaggtagta agcggattaa ctaagggaat aaaagatatt
      361 tttaccgctt tgtttactta gaatctgtct tccccaaaat ttatttttct aaaggtaaaa
      421 catagatagg cagataatac caattatccg ttacgaaacg ctgcttgaga attttcttta
      481 gatccataca acattttcta catctgacta aattggttcc tgattcatca agtaattgac
      541 tttctttgat aatatgataa aaaggcaatc aaggaggaag ataaatatgc ttaaagatct
      601 gaaccaattg atcgattaca tcgaagaaca tttgacagaa ggactttcct ataaaataat
      661 agccgaaaat acaggaatct ctgaatatca attaaaacgc atctttacgt ttattgcagg
      721 gatgtcgcta atggactata tcaaaaaccg tagattggct ttggctaatc gcgatcttct
      781 agatggatca agagtgacag aaacggcgtt tagatatgga tatgaaacag tcgaaggttt
      841 ttcaagagct tttcgtgatt ggtcaggata tctgccttca gaaatcagta gatacggcat
      901 acaaaaatct tttccacgct tgacattttt tattgatgtc agaggaggaa aatcgatgga
      961 atttcgtatc gaaaagaaag aacagtttta tgtggcaggt gtggcgaaaa aagttcctgt
     1021 caaatttgaa ggagtcaatc aagaaataca aaagttagcg cagacgatca cggagactca
     1081 aagggaagag atgcataagt taggagattt atatccaaat caagtaatca acgtttctta
     1141 tggatttgat ggagaccgct tggaagaaaa gggcagtttg atgcacatga ttggctttgc
     1201 tacgacaaaa gaaaatccgt acgctgattt agaaatgctt gctatagaag aacacacttg
     1261 ggccattttt cctaataaag ggccatttcc ccaaacttta caagatactt gggggaaaat
     1321 ctatgcagaa tggcttcctt cttcagatta tgggttagtc gaagctcccg agatctcttt
     1381 tacaaaatgg ggagaggatt tcagtaatgt ttacagtgag atttggattg cagtgaaagc
     1441 aaaaaaatag ttaaaaaata aaaatatccc tagaaaaaag agcgtaatcg tgtcatttcc
     1501 ctttaatttt atgtaagatg aacttaatat aatagaaagg gtgggagaaa tggcaaagta
     1561 taggatcgaa gaaaaaccag attttacggt gacgggtatt ggtgtggaat tgaagagtag
     1621 caatacggat tttgtcggtt tggctgcaga gaaacaagca tttgagcaaa aagttatcgg
     1681 taatggaaca atagatgcat tgaaacaagc tgctgaaaat cattatttct ttacagtcaa
     1741 cgaaccctat aacgggaaaa tgatgtacta tgtcggtgtt caaacgagtc aggatgttcc
     1801 aaacactacg cgaatcatcc cattcccaaa aggaacgtat cttgttgtaa gcgctttagc
     1861 agaatcgcct gaagaattaa gaaatgattt gacgaatacg gcctttgggc atgttttaag
     1921 agaagtagaa gattacgcct atgttggggg tccaaatgct gtagtcgaaa tgggagttac
     1981 caacgggaaa tatatgggag aaatctggat accggtagtc aataaaccat gataatatag
     2041 aggtcgtgaa agtagcgctt tgacctgagc attagagagg aaaaatggga aatagctttc
     2101 catattttgc atttttcaga actaatgccg agggtcagct acttgaacac cgtttatcaa
     2161 gacgtcatga cttcagcgct ttgacctgag cattagagag gaaaaagggg catgacaagg
     2221 cttttgtccc ccggagaata aaagaattta tgtagacgat tccgatcaga aaaatgcagg
     2281 cacagtagag agaattataa aaatctctct acgaggcctg tgttttctgg tcttttttct
     2341 ttttccaaaa cggctatttt atgtatcaga ctatcaagtg tgatcttagc catttcttct
     2401 tctgcctttt tctgcagccg agcgtagctt tcttctaaag cttcttggat attcgcacca
     2461 acgacgcaat ttggatttgt ttttggatcg acttggataa gctgtgtatt accttcgatg
     2521 ctgcgataga catctagtaa agaaatctga tctggacttt tggcaaggga aggctgcggt
     2581 tttccggttt ttgtaaagat caaaccggat tttttgagct gactcatgat tttacgtaca
     2641 tttgctgcat ttgtttcgat actactggca atcatctcac tagaaagtga tcgggtatct
     2701 tgaaacattt caatataggc aagtacatgg attgcatcac taaattgcag agaatacttc
     2761 atcagttgtc atcctttcat tttttttgct tgacttatct taccacactc tttatgataa
     2821 aggtgtaatt tataaatgta cagctgtaaa tcttaacaaa taaaaagaat agggagtggc
     2881 aaatatgcat tatttagtaa caggagcaac tggaggtttt ggtggttatg cgctggaatt
     2941 cttaaaaaag atggttccac aaaatgaaat ctatgtacta gtacgtagtg aagaaaaagg
     3001 agaagcactg aaagaagctg gatttaatat acgtattggt gattatgatg atttagactc
     3061 aatgaaagaa gcgttgaaag gaatagatcg attgcttttt gtctctggcg ttccaggaaa
     3121 tcgtcaagcc gaacataaga atgtagtgaa ggcagcacaa gcagctgggg tatcttatat
     3181 cgcttataca agttttgcag atgccgatca ctcgatcagt gtattagcgc cagatcacca
     3241 attcactgaa aaaatgatta aggattcagg tatttcccat acatttttac ggaataattg
     3301 gtatgtagag aatgaattac ctatcattgg gcaggcttta aaaactggtc aattcgttta
     3361 tgctgcagga aatggaaaaa caggttgggc attgaaacgt gaatatgcag aagctgcagc
     3421 aaaagtacta gtagaaagtg actctccaga aatcttagaa ttatccggca atctaactga
     3481 ctatgaagaa ttagccaagg cattagagcg tgcgacaggt aaagagttag aaattattga
     3541 agcaagtgat gcagcgtttg tcgaaaatct aaaagaagcc ggattccctc aagaagcagc
     3601 tgacatgttt ttagcgatcc aacatgatat taaaaaggat cagttagcta tttcctctga
     3661 tgatttagaa aaagtattgg gaaaaccatt aacttctctt gaagcagcat taaaagaaat
     3721 attggatcag gaataatgta taaagaggtt gtgaaaaagc cgattagttt tgagtatgaa
     3781 gaaaaatttt gtgaaaaagc tgttctgcat caagtaataa gaagagtcaa ataaaaaata
     3841 gctcttcata tgtttcgttt agctggactt attaccgcgg agttggcagg agaacgccgt
     3901 ttttattcgg aaaaaggagc tgagacaaga tttttgtctc agctcctgtt tgtttatcga
     3961 taaaaaagtc tggggaagac gatggctcag gctttgttgg aaacagagcc gattttatca
     4021 tttgctttta catcacgtgc gggttcgatt tctaagcttt ggactttgtc gccatttgtg
     4081 aatacaacga ccatatccgt gtttttgcct gcagattgaa tagcggcaag atcaactaaa
     4141 gcaatcaatt gacctcttgc gactttttga ccttcttcta catataaagt gaatggttcg
     4201 ccttttaagt caacagtgtt gatgcccata tgaagcaaga cttcgatacc agcatctgtt
     4261 tggattccga cagcatgttt cgttgggaaa atgtttgtga tcgtaccagc gattggagag
     4321 aagatttctc cattttctgg taatacagca tagccgtcac ccatcatttt ttccgcgaat
     4381 acatcatctg aaacttcagt gattggaatc agtttcccat ttgcgatagc gtaaatatct
     4441 tgttcgatcg tttcaccggc aaatgtttct gttttaacca cggtgtttgt ttgtgcaggt
     4501 cgtgctgcag caccaccatg tagttttgcc atctcatctg caacgaattg tacttctgta
     4561 cctacgatga cttgcgcatt ttttccgtcg atgatcttca taccaggtac gccagtggct
     4621 ttgattttat tttgatcgat caggcctgta tctttgactt gcaaacgaag gcgtgtagta
     4681 cagttatcga tcgaagttac attgtcggct ccgcctaatg cagcgtagat tttgcttgct
     4741 tgtgccgcat atttattgtc tgaattagtc gttgcagtat cgtcatctga aagatctgct
     4801 tcctcgcggc caggagtcat cagatggaat tttttgatag caaagttaaa gccaaagtag
     4861 taaatcactg ctgttactaa tccttggacg atcaacatca atggctgatt cgcaatcgga
     4921 tttttcaaag ataggaagta atcgacaaat ccggcactaa aggcaaatcc tgctgtccaa
     4981 tggaagaatg cactcactgc taaagaaatc ccagtgaaaa ttgcatgcaa gacatataat
     5041 ggccaagcaa cgaacataaa tgaaaattca agtggttctg tcacaccagt aaagaatgaa
     5101 gcaaaagctg ctgctagcat caaggaagct gtttttgctt tgttttctgg acgtgcattt
     5161 ctgtaaatag catatgctcc tgctggaagc ccgaacatca tgattgggaa aaatccagct
     5221 tgatacattc ctgttacgcc ttttgttcct tcagaagcca aaaatttacc aatatcattg
     5281 attcctgcta cgtcaaacca gaacacggag ttcattgctt gatgcaatcc tgtagggatc
     5341 agtaaacggt tgaagaagcc ataaagacct gcaccacccc agcctaaact caaaatgaat
     5401 ttagcaaaag aaactaaagc agagtaaacc ggaggccata cgagcagtag cactgcactg
     5461 atcagcaaca tgacaaaggc agccaagatt ggcactagac gcttgccgct aaagaaagat
     5521 aaagccatcg gtaattttgc ttcgtggaaa cgattataaa gagctgccgc aatcaatccg
     5581 gcaataatac caataaatac gttactgatc tgagcaaaag atggatcaac tgattctggt
     5641 ttgatattca ataaagtagc tactgaatca gtttttaaaa catttactgg aacttcaaat
     5701 gcgaccaaac ctgccaaggc agcagcacct gatttgtcca ctgacatccc tagtgctaac
     5761 ccaacggcaa acagcaaagg taattgacct aaaactgcgt taccgccagc ttgtaaaaaa
     5821 tgagcgacaa tgtcgttact ataagatgcc caccagtttc cgatccctac tagtaaagca
     5881 gcagcaggta agaccgcaac tggaagttga agggaacgtc ccattcgttg tagatatgct
     5941 ttcaattttt ttcctcctca tgattgttaa catttgtttg tacgattgcg cttcttaaac
     6001 gctcgatatg gatggccaag taacccagtt cttctttcgt gatcgcgagc tggtattctt
     6061 tggtcagcaa cacacggatt tttttagaaa tcttatagct ttctgggtat tttcgtttga
     6121 ccatttctac gatttccgtg tctaaaacag catattgttg cgcatgggtt cgttgtagaa
     6181 gcagtcgtaa atgattcacc agtcttgaat aattcagtga aaatggttct gaatggatat
     6241 ctgtttccaa ttctgattcc actaaatgga tgatgtcaga tacaatcgtc acttcacgga
     6301 tactgcgatg gttattgttc cgaccgcttc gtccgctgtg aatatggatt gcgatatatc
     6361 ctgcttcgtc ataagtatat ggaatcccaa gctcttttgt cagatactct actgcccatt
     6421 gagcaatact gaattcatca ctgtaaagca cttcgatttc tcttaaaagt ttgttgcgga
     6481 tgacgatacc gttattaatg ttctccgcag aaaaagcaat gtggtcggtc aaagcgatca
     6541 atacatgttc attcaacttt tccatcaaaa cagtttccgc atgatcaatg atcgtttctg
     6601 ctgcaaataa aaagcgttca tcaatctgat taagtaaatt ttgcaacttg atttgacctt
     6661 ctggttccat gacaaatatc cgttcgacat cacgatcaaa aatcaaatca tttctttttc
     6721 tgtcaaatcc aatccctttt ccaatagcta ctttttcctg tccaccgtca tctaccaata
     6781 ctgcattttg attaaagatc tttttgattt tcatgcgcgc accctttttg aagatttggt
     6841 tttcacgttt gctagaccag ttgattgcgg ccaaacaaaa atagacacga aaaacctcct
     6901 agggaaacca tgttcctcag agatttttca tgcctagtat gatctagtca catgaaacga
     6961 attattgtta acgtaatcaa ttttatatag acagttatgt tttgtcaatt ctttttttat
     7021 aattatagta atttactgga attatcagaa ataaaaggaa gaaataaatt aagaaactta
     7081 tgttatttaa tcgttggatc ttttgcttat cttattcgtt gttaaaaagc tgaaagcgaa
     7141 ggaaaaaaac tctacttaac tgtatttctc cgttaggaca tctaaaaatt tacgagcagc
     7201 aggagaaaga ggctggtctt ttttccaaat gagattcagt gaagcttgta atggcggatc
     7261 gagtgggatg aatatttttt ccgtcccata agtattgatg atcccatcta gagctaatgc
     7321 accaaaagtc ccgttatcga ccatcaaaga agcattgtac aataagttat atgttccgac
     7381 gatcgataac tgatctatgc tgaaacctag ccattcagct agttgactgt ccactgacgt
     7441 ttgccgggaa atgatcaaag gaacagtttt cagttcctgc ggatggatga cggtttgctt
     7501 tgctaaagga tgatttcgat gtatcaaaag tccccaacga tcttgggctg gcagtcgaaa
     7561 tgactggtat ttttgtttgt ttgtcggttc gattacgagt ccaaaatcaa gtaatccgct
     7621 atccagtttg tctatgatat catctgcatt cccgctatat aaatgaaact ggatatcagg
     7681 atgcgccttg atcaacgaat gaaggctatc agaaatgaaa gagattgctt tagtttcgcc
     7741 tccgccaatg tagatttctc cagagatgat tgcgtcattt gttaaattgt tcgccgtttt
     7801 ttctaccaac gacaagattt cttttccttt tgaataaaga tatcgaccag aatccgtcaa
     7861 ctgtatctct cgggagcccc gagtgaaaag tgtcacacca agttcttctt ctaaatcttt
     7921 cagttgtttt gagagagtag gttgcgtgat atgcagagat tttgctgctc ggctgatatt
     7981 tttttctcta gctactgcta aaaaatacgt cagtatacga atatccattt tttccctcct
     8041 gtttttaaaa aagcatacca ctaagtatag ttaaaaggaa tagttaagta tgttttataa
     8101 gtattagaca tgataagccg tccgttataa agtaaggata aaaggaggga gcaaaatgac
     8161 aagcctcatc gaaaaacaaa tcgtcaataa agagattcga gtaacggatc cgctctttga
     8221 agtgatccat cagatccaag ctgaaaacga agagccttta gcagcgctga atactgggta
     8281 ccatgaacca gcagatatcc gcaaacggct agaaaatatc attagtgaca aagtagatga
     8341 tacggtgact gttctgctgc ctttttatac agatttcggc aaacatatta gtatagggaa
     8401 aaatgtcttt atcaatcgtc aagtgatgtt cgttgactta ggtggcatct gtttagaaga
     8461 ttcagtattg attggtcctc gtgtcaacct catcacggtg aaccatctaa ccgatccaaa
     8521 agaacgaaga ggcttgtcag taaagccaat ccacatcaag aaaaatgctt ggatcggagc
     8581 aggagcaact attctgccag gcgtaacgat tggcgagaat gccattgttg cagcgaatgc
     8641 aactgttacg aaagatgtcc cagacaatac catcgttgca ggaatccctg ccaaaatcgt
     8701 caaacctgtc gaacggatta tttgaacagt aagatgaaat aagagatagt gagtataagc
     8761 aagagatttt tcaaaacagg aggagaatag aatgaatgat ttatcagaaa aagtgattgt
     8821 catcatgggc gcttcaagcg gaatcggtga agcaacaacg aagctattag cagaaaaagg
     8881 ggctaaacta gttatagctg cacgccgaga agatcgccta aaagcaataa aagaatcttt
     8941 accagaagct gagctatata ttcaaacagc agatgtaaga gattttgcac aagtccaagc
     9001 agttatcgat ttagcgatgg aaaagttcgg tcgaatcgat gtattgtaca ataatgcagg
     9061 aatcatgcca accgctccat tagttgaagg tcaccgtgat gaatggcaaa atatgcttga
     9121 tatcaacatc atgggtgtgt taaatggaat ttccgccgta cttccaatca tggaaaaaca
     9181 aaaatctggg catatcattt caactgattc tgtggcggga catgttgttt atcctgattc
     9241 cgccgtttat tgcggaacaa aatttgctgt ccgtgcaatc atggaaggat tacgtcaaga
     9301 gcaacggcaa aataacatca aatctacgat catctctcca ggagctgttc aaactgaatt
     9361 atatcaaacc atcagcgaca aaaaggctgc tcttgaactt catgaagcgc aaaaagaatg
     9421 gggattgacc tccgaagata tagcttctgc tgtagcattt gcgatagaaa cgccagaccg
     9481 gatgtctgtc agtaacatga ttatccgtcc gacgacacag gaagtataat ccataacgga
     9541 taatatccaa agagaattac ttgttaaata gttattcaaa cgaccatcaa tttaaggcgt
     9601 gaatggagaa gaatattctt catttacgtc tttttatgta gaaaaagcaa atttatcaaa
     9661 aagaaacaaa tgttatttaa atatggacat tgacttgctt ttactttttt agttacactg
     9721 ttatttagga aaagtttcat aattcaaagg agaaaaaaga tgaaagcaaa gaatttatta
     9781 tttagcgtta ttcttttagg aaatctagca gttgccaaca caacagtgct agctgaagaa
     9841 tcgacagaaa ctgtcgcaac tcagacatcg agttcttcag aaacaggaat tgaaaacgat
     9901 acagagagta ctttgcagtc agctactgtc tcagaaaata caatttcaac cgaagaacca
     9961 acaactgaaa caacagaaca aacacaaacc gaagacagta catcagcaga agctcagaac
    10021 aatccagaga atgcagaaga aatcacgaac caattgacag atgggcaaac attgacacat
    10081 acccatcaag cagatttgtc tgaattcgaa aactatactg gctatgcttt ttatgctcgc
    10141 tcatctgcta attctcaaca agcgttcatt gatagtattg cctcaactgc acaaagtttg
    10201 gcaagtgcca atgacttata tgcatcagtc atgattgctc aagccatcgt tgaaagtggc
    10261 tgggggaaca gtactttggc ttctgcccct aattacaatc tatttgggat taaaggaagc
    10321 tataatggcc aatctgttac gatgcctact tcggaatatg tgaatgggca atggattacg
    10381 gtgaatgccg cttttagaaa atatccttcg tacaaagaat cattgcaaga taacgtaacg
    10441 gtattgaaga cgacttcttt tcagccaggc gtttactact attccggtgc atggaaaagc
    10501 aatacgaact cttacaaaga tgctacggct tggctaacag gtcgttatgc cactgcgcct
    10561 aattatggtt ctacattgaa caatgtaatc gaaacctata atctgacaca gtatgataca
    10621 gcaccaactt caacttctcc gatgtatcgt ctgtataacc gtcatacagg agaacattta
    10681 tatacattga atgccggaga aaaaaattat ctgccaactg taggctggga atatgaaggg
    10741 attgcttggc aagcaccaaa cagcggacaa cctgtctacc gtttatacaa tccttacagt
    10801 ggagaccatc attacacaat ggcacaatct gagatcaact ctttaacaaa aatcggttgg
    10861 cgttatgaag gtctaagttt ttattctggt ggttccaagc caatttatcg actatttaat
    10921 ccaaatgaaa aaactggtac ccatcactat accttgagtg ctaaggaaag agattggcta
    10981 acaccgatgg gctggagata tgaaggaata ggattttatg gttattaaaa agaggtagtg
    11041 aaaaagctgc cctgcatcaa gtagtaagaa cagccaaaca aaaacagatt ttcatgtttt
    11101 ttgtttggtt gaacttatta ccgcagatgc aagtttttga acaccgttta ctcaaaaaag
    11161 gttgtgacaa catcttgtca taactttttt attactgttt aagcatactg atccaatttc
    11221 taatattcga cgggattctt gcaaaaggcg atttcggaaa tttttggcgg tgtaaaattt
    11281 tctactgcag aagagctatc ttctatagtt tgataagaat caataatctt ggttttagcg
    11341 ttgtgctttc tttatcattt tttccgttca ttctacaaaa aatttctttt ctctatactt
    11401 ttctattgag agcgtttttt ttattacaca gctgattctt tgactttttt ctttttttat
    11461 tctattattg acaagtcaat cgttgacgag tcaattatta gaaaggagga aattatgtct
    11521 ttagaagaga ttagtcacct tttatatcaa atcaaattag ccgatcaaaa aatcgtccag
    11581 atctttgaag aaaatatcgg tataagtctt actagatacg agatcatgat gagactgaaa
    11641 gaagaaaatc cattgctaca aagcagtttg caagaaaagt tgcagattga tcaaggagca
    11701 gttactcgtc atctgaaggt attggaagca aatggctatg tttcgcgtca gagaaatcct
    11761 cagaacaacc gggaagtgta tgtagcgcta acagaaaaag cccagaagaa aatcgagcat
    11821 tgtgaaatgg accataaaga tacaggttcc gtgctatccc cggctttcag cgatgtagaa
    11881 atgaaacaat ttctaacact tttagataaa ttgaataccc aactttgcaa tataacaaca
    11941 gaaacgaaag aggttaataa taatggaaca aaaaaatgat ttttcaacaa ttatgactgg
    12001 tagaagatcc gttcgcgtat atgatgaaac atacaaaatc ccacacgaag aaatgctgga
    12061 aatgatcaaa gaagcgacga ctgcaccttc ttctgtcaat atgcaaccat ggcgctttgt
    12121 ggtcgctgaa agcgaggaag caaaagaaat cttgcgtcct ttgatccgtt tcaatacgag
    12181 acaaaatgat acttcttctg caatggtcat gatttttggc gatatgcaat gttacgaata
    12241 tggcgaagaa atctataacc aagcttacga atcagggaag atgccaaaag aagtcagaga
    12301 tcaacaatta gctgcgatca ttccttatta caagagtttt tcaagagaag aaatgaacga
    12361 tgtagtcaaa gtcgattcta gcttagcggc tatgcaattc atgttagttg cgcgtgatca
    12421 tggctatgaa acaaatccaa ttggtggatt cgaagcagat cagttggcag aggcatttgg
    12481 tttggataaa gaccgttatg ttccagttat tattttatca ataggaaaag cggtagaaga
    12541 aggttacgag tctgttcgtt tagcaccaga aaagatcact acgttcgaat aaaaggtttt
    12601 cagttactat tcagtagcta ttgctttttt tgaatcgtgt gtggtaacct aataaaaaag
    12661 gaggtcggaa aaatgactgc atcgatgaaa ttacgtttcg aacttttgaa taacaactaa
    12721 ataaattcaa aagacatgta taaacaacaa tttcatggat ggagtatgtc cattttttcg
    12781 aacgacagta gaataggtga aagctatgac agatttggtg atttagtcat agttatttcg
    12841 ttcagagtag gaaaagatgt gatgcagttt ttttgcaaac agatttttct gctctttttt
    12901 agtatcgata attgttgttt tacatgaaat aggagtgtaa aacatatgga aaatttagca
    12961 gtaaatataa caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct
    13021 cttcgtgtct atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg
    13081 actttgctca aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag
    13141 atcaccttca actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg
    13201 gaattagcca gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga
    13261 ggggaagaac gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt
    13321 ctagacgagc caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt
    13381 cgttattatt atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta
    13441 gcatcgaaaa tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt
    13501 gcctatctca ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc
    13561 atgaaagaga aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag
    13621 ttggaaaaag tgtccagtaa aaagaaaaag caacaaatca gaccggatcg gttgtcttcc
    13681 tctaaacaaa aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga
    13741 agactccaaa aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca
    13801 gtaccaaaat ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg
    13861 gaaagaagtg ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa
    13921 atcgcgattg tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa
    13981 caaggagaag gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt
    14041 tatcaaatga catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca
    14101 gaatcgcttg tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa
    14161 ccgttatgta cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact
    14221 aagccaagta atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc
    14281 gaagctttag agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat
    14341 tcttactttg tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa
    14401 aaagtactta cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga
    14461 gtggtacaga ttgcctacaa ataaaaaaga aggaataatt tttacaacaa tgatgtgttt
    14521 tttgatggtc ttggggatga gcgcgtataa tttatggctg catcaagatc ttcatttatc
    14581 aagtttgttg attggtctag tgcctggatt cgtggtggct ttcattttgg atgtcttcat
    14641 catcggtgtg ctggcgaaaa aaattgcctt taaactacca gtaaatccag aaagcaaggt
    14701 ccaaatgatc ttaactattt catgcttgat ggttttagga atggtgacat gtatgtcttt
    14761 attcggtgtg atcatggaac aaggaatacc tgatcaatta ttctctgctt atatgaatgc
    14821 ttggaaaatg aatgtgattg ccgctttacc gttgcagttg atcatcgttg gcccgctttc
    14881 aagaagtgtg ctaaaagcaa ttcaaagtaa tgaatgatgg aacactgtta tttgaaaaaa
    14941 ggccttggca tgctttttgt catagtctct tgaatgcagt tctagaaaaa tagtgatccg
    15001 aacgataggt tttggcctgt cgttcggatc gctttttctt ttccaactgt attgctcttt
    15061 tattgaagat aatttgctct tcaatagata tttctagaaa ggttctgttt atcccttact
    15121 caaaaaactg ctgcatttgt tctcttactt gctgacacca ttggagttcc tgctgacaaa
    15181 gcataattct gcgctggatc acaacataac gtccaaattc ttgtttcttg tctagcgaga
    15241 ggtcattcaa ttttttttga tttttttgca tgattttgtt cagctgttgt tctctagtga
    15301 atagcagtcc agtagctgtt gttttatcga gcgtcgaaaa agcatagatt tttgctagaa
    15361 attcatcttt ttgcgtagga gcgggagttt cctctttgat ccattcctct acaagagcac
    15421 gtccttcatc agtcaagtgg tagatctttt tttgagagtg ctcgtctttg tccaaaacgg
    15481 tcacataacc ctgctggtct aacttggcaa gcgttgtata gatctgactg tgatgggctt
    15541 cccaaaaaat tgtgatcagc tgcttcaatt catacccaga acgtggtttg gataaaagca
    15601 cgcataataa tatataggct aaagtattca taaaaatcct acctctcgtc ttattttctt
    15661 tattatatca cagtaaagtt tccacgtttt acatgtgcat atttacatgt aaaataaatc
    15721 atgtatgatg ctaatagaga gaaagggatg gaaatattgg agaatcaaat agatatacaa
    15781 gaacaaacaa atgaagaaac aaaggggaaa agttcagcgc tagttgtagc agctacctgt
    15841 atgatcgctt ttatgggagt gggtctagta gacccaatct taaaaacgat tgctgcacag
    15901 ttaaacgcta cgccagctga aacgaccctg ttatttacta gttacatgtt agtcactggt
    15961 gtggtgatgc tatttacagg tttcttatct agtcggatcg gtttgaaaaa acattgatga
    16021 caggattgtt tatcatcgtt gtattcgctg gtcttggcgg attatctggg aatatcggaa
    16081 tgctgattgg gttacgtgcc ggctggggtg tcgggaatgc tttatttgtc tctactgctt
    16141 tagcaacgat cgtcagtatt ttagttggta acacagaaaa agcaatcatg atgtatgaag
    16201 cagcacttgg ctgcggaatg gctgttggcc ctctagtcgg tggggtcttg ggcagtggtt
    16261 catggcgtta tccatttttt ggggtagcat ttttgatgtt ttgcggtttt atagccctag
    16321 cggtactttt acctgaaact gagaaaccaa agagaaaagt aggcctttgg gaaggtgtca
    16381 aagcgttaag caatcataaa cttcgttcag ttggactaat cgcattgtta tataatttcg
    16441 gattctttac tttacttgcg tatgcgccat ttttattgat tggctattct gaattagaag
    16501 tcggttttgt attttttggc tggggtgtgc ttctggctat tgcttcgatt ttcgttgcac
    16561 cggctcttga gcggaagttt acgacttatc ggacaatttt agcagcattt gtcttattca
    16621 ttatttgttt gctgttatta ggagctggaa cttctatgcc agtccttgta ccgatcagca
    16681 ttgtgttagc aggatttttc caaggaatta tcaatacact gttgacgacg attgctatgg
    16741 aaataccagg tctgcaacga aacgttgctt cgtccagcta tagttttgtc cgctttttcg
    16801 gtggagcttt agcaccattt attgcaggtg tgattggtga acatattggt gaaagctatg
    16861 cgttttattt tgcaggtgcc attgttctta tcagtgtatg ttgtatcatt tatcaccgta
    16921 attattttgc tacaaatgaa gaagaataag aaatcagagg ttgtgaaaaa gctgtcctgc
    16981 atcaagtcat aagaa
//
LOCUS       DACEKH010000251        11422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.guided.089, whole genome shotgun sequence.
ACCESSION   DACEKH010000251 DACEKH010000000
VERSION     DACEKH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.guided.089"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            60..977
                     /locus_tag="IW662_002856"
     CDS             60..977
                     /locus_tag="IW662_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0867624.1"
                     /translation="MPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKNLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            980..3163
                     /locus_tag="IW662_002857"
     CDS             980..3163
                     /locus_tag="IW662_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0867625.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3160..4161
                     /locus_tag="IW662_002858"
     CDS             3160..4161
                     /locus_tag="IW662_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0867626.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4176..5090
                     /locus_tag="IW662_002859"
     CDS             4176..5090
                     /locus_tag="IW662_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0867627.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5335..5451
                     /locus_tag="IW662_002860"
     CDS             5335..5451
                     /locus_tag="IW662_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0867628.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5467..7386
                     /gene="tet(M)"
                     /locus_tag="IW662_002861"
     CDS             5467..7386
                     /gene="tet(M)"
                     /locus_tag="IW662_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0867629.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7484..8857
                     /gene="tet(L)"
                     /locus_tag="IW662_002862"
     CDS             7484..8857
                     /gene="tet(L)"
                     /locus_tag="IW662_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0867630.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9421..10683
                     /locus_tag="IW662_002863"
     CDS             9421..10683
                     /locus_tag="IW662_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ0867631.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10928..11410
                     /locus_tag="IW662_002864"
     CDS             10928..11410
                     /locus_tag="IW662_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0867632.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNGSVAKF
                     "
BASE COUNT         3772 a         1906 c         2388 g         3356 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcaatctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgta attatgaaaa ataccaaaga ttctgaatca ctggctattg
      181 atattttgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtgcgagg gttgatgaaa gtgattgaag
      301 aattacgggt tgagaatacg ccactaagta ccagcatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcccat ctgcttttta gtgatggtta tgtagagcag tctatcagcc
      421 ttgaaaaaca actgaacatt atacaggttg ccgacttggt acttcctgac aaggaaactt
      481 cctttgagga atataccaca atggagttac tatcggtagc aatgctgatt gtcattagta
      541 cctttgcgtt ggactttatc catacagacc gaagtatttt caagattgta gacttagacg
      601 aagcatggag cttcttacag gtggcacaag ggaaaacact atctatgaag ctggttcgtg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagat gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcctt tcgttccact gaccttaacg
      781 agattaaaaa aaaccttgcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggctac cgtggaactg gtcggacgga attggaaaac aagtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ctttatgttt ccaatgcgac
     1321 gggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggcaagaat atgcagaccc ttgcaggggt tacgcctagt ggactatcaa cagagggttt
     1441 ctatgttgga ttccttttac tcttgatttt ggttcttggg gtttatgttg cctatacagg
     1501 actgataaag agagaaacca caaaggcaat tcatgccctc atgaattttg tgctggtttt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagcaacg ctagtttgtc actgggtaca aaaattgtca tgccccattc
     1681 agatagtcaa ggcaaggaca gtgtggactt gatacgtgac agcctatttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatataa cagttcagac attgaaagta ttggtattga
     1801 ccgtgtggaa agcctgctct caaccagtcc agattccaac aatggggaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagatagaag caataccaat atgacgatta caaagacgat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttt aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtatga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gctccgtgac
     2401 tgctttaggg gctgggtctg ccattgccag tgctacgggt aaaaaaggac agtcgggttc
     2461 gggaagttct gcgagtacac aagcagatca ctctcgacca aacgggcagg aaaaatcaac
     2521 actaggaaag cgtatcggtc aaaccatcgg tacagtagct gataccaaag acagaatggt
     2581 agatactgct ggtaatttga aagaacaggt taaggatttg ccgaccaatg caagatatgc
     2641 agtatatcaa ggaaaatcta aagtgacgga taatgtccat gatttaacca gaagtatttc
     2701 tcaaaccaaa gcggataaag ccagtggacg caagaaacag caggaacaaa gacggaaaac
     2761 cattgcggag cgtcgctctg aaatggaaca ggtcaaacag aaaaaacagc cagcttcttc
     2821 cgttcatgaa agaccagcca ctaagcaaga acaatctcat gatggacaga cttcaaggca
     2881 aacaactgta caggcttcct atagggaatc tcaacaagcc aaacaagagc gtccaacagt
     2941 taagtccgat tcttcaagtt taaaaacgga acgtcaaagt aatacagttc aagaaagaac
     3001 ggttcaaaag ccagtaactt caactgcacc aacagataga gcttcacaac gttcaatcac
     3061 aaaagaacgt cagtctactg ttcaaagagt accactacaa aatacaaaaa gtagaccacc
     3121 aatcaaaacc gccaccatta agaaaggcag taagaaacca tgaagctgaa aactttagtg
     3181 attggtggtt cgggattatt cttgatggtc ttctcactgc ttctgtttgt cgccatttta
     3241 ttttcagatg aacaagatgg cggtttttcc aacattcact atggcggtgt ggatgtttcc
     3301 gcagaagtgc tggctcataa gcctatggta gaaaaagttg ccaaagaata tggaattgaa
     3361 gaatacgtca atatacttct tgcgattata caggtggaat cgggtggtac agcagaagat
     3421 gttatgcagt cctcggaatc cctcggtctt ccacctaatt cattgagtac agaagaatcc
     3481 attaagcaag gtgtgaagta tttcagtgag ttattagcca gtagcgaaag gcttggtgta
     3541 gatttagaat cggttatcca gtcctacaat tatggtggtg gtttcttagg atatgtggct
     3601 aatcgtggga ataaatatac ctttgaactg gctcaaagtt tctcaaaaga gtattcgggt
     3661 gacgaaaaag tgtcttaccc caatcccata gccataccta tcaatggggg ctggcgatac
     3721 aattacggga atatgtttta tgtgcaactg gtaacgcagt atcttgtcac aacagagttt
     3781 gatgatgata cagtacaagc catcatggac gaagctctga aatttgaagg ctggaagtat
     3841 gtctatggcg gagcttcccc gactacctct tttgattgta gcggactgac acaatggacg
     3901 tatggaaaag ctggaatcaa cttaccacga acagcacaac agcaatatga tgtaacccgg
     3961 catatcccat tattggaagc aaaagccggc gatttggttt tctttcattc tacctataac
     4021 gctggctctt atattactca tatcgggatt tatttaggcg ataaccgtat gtttcatgca
     4081 ggcgacccta tcggttatgc cgacttaaca agctcttact ggcaacagca tttagtggga
     4141 gcaggacgaa tcaaacaata agaaaggacg attcaatgat gaaattcgga aaaaatcaga
     4201 atacagaaaa acagacacca aaggaaaaga aacctcgtgt ctataaggtc aatcctcgta
     4261 aaaaggttgt gattgtccta tgggtacttt taggacttag tttcagcttt gcgatattca
     4321 agcactttac agccattgat acccatacca ttcatgaaac caccatcata gaaaaggaat
     4381 atgtcgatac tcatcatgtg gaaaattttg tagagaactt tgcgaaagtc tactattcat
     4441 gggaactaaa cgacaagtcc attgataatc gcatagaaaa cctgaaagca tatctgacag
     4501 aggaacttca agctctgaat gtcgatacag ttcgtaaaga tattcctgta tcgtcttctg
     4561 taaaaggatt tcagatatgg acggtagaag cagatggcga caatcaattt gatgtaacct
     4621 acagtgtaga ccaactcatt acagaggggg aaaatacaaa gaccgtccac tctgcttata
     4681 tagtgagtgt ctatgtagac agtactggaa atatggtcct cattaagaat ccgaccatta
     4741 ctagcatacc aaagaaatca gactataaac caaaagctat tgaaagtgag ggtacggttg
     4801 attccattac aaccaatgaa atcaatgagt ttttaacgac gttcttcaag ctctatccta
     4861 cagcgacagc cagtgaactt tcctactatg tgaatgacgg gatattaaaa ccaatcggaa
     4921 aagagtacat ctttcaagaa ctggtaaatc ctatttacaa tcgtaaggat aatcaagtca
     4981 cggtatcgct gacagtggag tatatcgacc agcagaccaa agcaacgcag gtatctcaat
     5041 ttgatttggt acttgaaaag aacgggagta attggaagat tatagaataa caaatattgg
     5101 tacattatta cagctatttt gtaatcacgt actctctttg ataaaaaatt ggagattcct
     5161 ttacaaatat gctcttacgt gctattattt aagtatctat ttaaaaggag ttaataaata
     5221 tgcggcaagg tattcttaaa taaactgtca atttgatagt gggaacaaat aattggatgt
     5281 ccttttttag gagggcttag ttttttgcac ccagtttaag aataccttta tcatgtgatt
     5341 ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca taaaaatccc
     5401 agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg aatggaggaa
     5461 aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcggg aaaaactacc
     5521 ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa
     5581 ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca
     5641 ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat
     5701 atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg
     5761 atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa
     5821 atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca
     5881 acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta
     5941 gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta
     6001 atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg
     6061 gaactcgaac aagaggaaag cataagattt cagaattgct ccttgtaccc tgtttatcat
     6121 ggaagcgcaa aaagcaacat agggattgag cagcttatag aagtgataac gaataaattt
     6181 tattcatcaa catacagaaa gaagtctgaa ctttgcggaa atgtcttcaa aattgaatat
     6241 tcggaagaaa gacaacgtct tgcatatgta cgcctttatg gcggaatcct gcatttgcgg
     6301 gattcggtta gaatatcgga aaaggaaaaa ataaaaatta cagaaatgta tacttcaata
     6361 aatggtgaat tatgtaaaat tgataaggct tattccgggg aaattgttat tttgcaaaat
     6421 gagtttttga agctaaatag tgttcttgga gatacaaagc tattgccaca gagagagaga
     6481 attgaaaatc cgctccctct gctgcaaaca actgttgaac cgagcaaacc tcaacaaagg
     6541 gaaatgttac ttgatgcact tttagaaatc tccgacagtg acccgcttct acaatattat
     6601 gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg
     6661 attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc
     6721 atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca
     6781 aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg
     6841 cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg
     6901 gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa
     6961 atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga ttttcggatg
     7021 cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt gttagagcca
     7081 tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcata caacgatgct
     7141 cctaaatatt gtgcgaacat cgtagacact caattgaaaa ataatgaggt cattcttagt
     7201 ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg atttaacttt ctttacaaat
     7261 ggacgtagtg tttgtttaac agagttaaaa gggtaccatg ttactaccgg tgaacctgtt
     7321 tgccagcccc gtcgtccaaa tagtcggata gataaagtac gatatatgtt caataaaata
     7381 acttagtgta ttttatgttg ttatataaat atggtttctt gttaaataag atgaaatatt
     7441 ttttaataaa gatttgaatt aaagtgtaaa ggaggaatta attatgaagt gtaatgaatg
     7501 taacagggtt aaatacaacc aaattttaat ttggctttgc gttttatctt tttttagcgt
     7561 attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt ttaataaacc
     7621 acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca ttggaacagc
     7681 tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat ttggaattat
     7741 aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt ccttacttat
     7801 tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg taatggttgt
     7861 agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta ttggatcgat
     7921 agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc attatattca
     7981 ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt ttcttatgaa
     8041 attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa ttatactaat
     8101 gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt cttttcttat
     8161 cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa cagatccttt
     8221 tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt gtgggggaat
     8281 tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga aagatgttca
     8341 ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa tgagtgtcat
     8401 tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat acgtgttaaa
     8461 catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt tagaaacaac
     8521 atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt tcaccaaaac
     8581 agttatatca acaattgttt caagtagctt gaaacagcag gaagctggtg ctggaatgag
     8641 tttgcttaac tttaccagct ttttatcaga gggaacaggt attgcaattg taggtggttt
     8701 attatccata cccttacttg atcaaaggtt gttacctatg gaagttgatc agtcaactta
     8761 tctgtatagt aatttgttat tacttttttc aggaatcatt gtcattagtt ggctggttac
     8821 cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat caactttggg
     8881 agagagttca aaattgatcc tttttttata acaggaattc aaatcttttt gttccattaa
     8941 agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt ttattttgag
     9001 tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc ttgctgagtc
     9061 tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt ttaaaggtgg
     9121 agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt tttaaacgag
     9181 cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt gtttcttttt
     9241 tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac tgccgacagc
     9301 ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta cttggaagtg
     9361 gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag cgatttacat
     9421 atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa aggcatgcaa
     9481 tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca tgagcgaaca
     9541 cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg tgtcaaagaa
     9601 attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt tcttgtaaat
     9661 gagttgctag taacatctga ccgagatttt tttgagcaac tggatccagg agaacaaaaa
     9721 cgattttttg aggaaagtta taaattattt tccgaacgat atggcaagca aaatattgct
     9781 tatgcaacag ttcataatga tgagcaaacc cctcacatgc atttaggtgt tgtgcctatg
     9841 cgtgatggaa aactgcaagg aaaaaatgtg tttaatcgtc aagaactgtt atggctacaa
     9901 gataaattcc ccgagcatat gaaaaaacag ggttttgagt tgaagcgtgg tgaacgtggc
     9961 tctgaccgta aacatattga gacagctaaa tttaaaaaac aaactttgga aaaagagatt
    10021 gattttctag aaaaaaattt agcagttaaa aaagatgaat ggactgctta tagcgataaa
    10081 gttaaatcag atttagaagt accagcgaaa cgacacatga aaagtgttga agtgccaacg
    10141 ggtgaaaagt ccatgtttgg tttgggaaaa gaaataatga aaacagaaaa gaaaccaacc
    10201 aaaaatgttg ttatatcgga gcgtgattat aaaaacttag tgactgctgc gagagataac
    10261 gataggttaa aacagcatgt tagaaatctc atgagtactg atatggcgag agaatataaa
    10321 aaattaagta aagaacatgg gcaagttaaa gaaaaatata gtggtcttgt agagcgattt
    10381 aatgaaaatg taaatgatta taatgagttg cttgaagaaa acaagtcttt aaagtctaaa
    10441 ataagcgatt taaagcgtga tgtgagttta atctatgaaa gcactaagga attccttaag
    10501 gaacgtacag acggcttaaa agcctttaaa aacgttttta aggggtttgt agacaaggta
    10561 aaggataaaa cagcacaatt ccaagaaaaa cacgatttag aacctaaaaa gaacgaattt
    10621 gaactaactc ataaccgaga ggtaaaaaaa gaacgaagtc gagatcaggg aatgagttta
    10681 taaaataaaa aaagcacctg aaaaggtgtc tttttttgat ggttttgaac ttgttctttc
    10741 ttatcttgat atatatagaa ataacgtcat ttttatttta gttgctgaaa ggtgcgttga
    10801 agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt gcacagaaaa
    10861 accccatctg ttaaagttat aagtgaccaa acaaataact aaatagatgg gggtttcttt
    10921 taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg ttttagtgga
    10981 caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta atgttgatta
    11041 ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag attgtgctga
    11101 aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc gagtgtggtt
    11161 ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac atggcattca
    11221 gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt ggttgtttct
    11281 cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt tgtcagatat
    11341 ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatg gttctgttgc
    11401 aaagttttaa ataaagaata aa
//
LOCUS       DACEKH010000252         5246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.guided.132, whole genome shotgun sequence.
ACCESSION   DACEKH010000252 DACEKH010000000
VERSION     DACEKH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.guided.132"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            109..192
                     /locus_tag="IW662_002865"
     CDS             109..192
                     /locus_tag="IW662_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ0867633.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="IW662_002866"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="IW662_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0867634.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="IW662_002867"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="IW662_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1647..2312
                     /locus_tag="IW662_002868"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="IW662_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="IW662_002869"
     CDS             2348..2593
                     /locus_tag="IW662_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0867635.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="IW662_002870"
     CDS             2696..3565
                     /locus_tag="IW662_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0867636.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..4280
                     /locus_tag="IW662_002871"
     CDS             3546..4280
                     /locus_tag="IW662_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0867637.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4313..5225
                     /gene="ant(6)"
                     /locus_tag="IW662_002872"
                     /pseudo
     CDS             4313..5225
                     /gene="ant(6)"
                     /locus_tag="IW662_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
BASE COUNT         1872 a          766 c         1037 g         1571 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     4141 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     4201 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     4261 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     4321 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gaacgtattc
     4381 gaattgtgac ccttgagggg tcacgcgcaa atattaatat acctaaagat gaatttcagg
     4441 attatgatat tacatatttt gtaagtgata tagaaccgtt tatatctaat gatgactggc
     4501 ttaatcaatt tgggaatata ataatgatgc aaaagccgga ggatatggaa ttattcccac
     4561 ctgaagaaaa gggattttcc tatcttatgc tatttgatga ttacaataaa attgatctta
     4621 ccttattgcc cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc
     4681 taattgataa agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg
     4741 taagaaagcc aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac
     4801 cttatgttat taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc
     4861 agattgttcg ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct
     4921 ttaaattaag tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt
     4981 gggagaaact tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat
     5041 ttctatgcca tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg
     5101 cctatccgga gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca
     5161 ctggtaaaac cggctgcctg gatagcacat atgccgctga tatagaagag agactaacta
     5221 tatagagtca agtaaaaatt agccaa
//
LOCUS       DACEKH010000253         5008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.guided.133, whole genome shotgun sequence.
ACCESSION   DACEKH010000253 DACEKH010000000
VERSION     DACEKH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.guided.133"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..2193)
                     /locus_tag="IW662_002873"
                     /pseudo
     CDS             complement(43..2193)
                     /locus_tag="IW662_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2339..2914
                     /locus_tag="IW662_002874"
     CDS             2339..2914
                     /locus_tag="IW662_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0867638.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3128..3823
                     /gene="vanR"
                     /locus_tag="IW662_002875"
     CDS             3128..3823
                     /gene="vanR"
                     /locus_tag="IW662_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0867639.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3801..4955
                     /locus_tag="IW662_002876"
     CDS             3801..4955
                     /locus_tag="IW662_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0867640.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1564 a          940 c          970 g         1534 t
ORIGIN      
        1 tagtttgaaa tgcaattttc ttctttcagc tttttatttc ccacttgtaa atcggtagat
       61 ggtggctcct tttccagttc cataatgtgg atttgcatct gcatgtagtg atgaaacacc
      121 tagctgcatt ctcataccat ctgacgaaga tgttgtaccg tcgccccaat agaaaggcaa
      181 ttgtaattta tgatgaaagt ttactaatat ggcttgggct ttattcatgg catcttcata
      241 catgcgccat tgagatacat tggctagttg cttatatgta agtccgggtg tggcttcggc
      301 catcttgctc aagccaatat tcattcccat tcctaaaagg gcagccatga taatgattgt
      361 ttcttcctta tctggttttc gattattgga agcatgagtg aattgctcat gaaatcctgt
      421 tatatgggcc acatccatga gtaaatcagt taattttatt cttggtagca tctgataaag
      481 gcttgcacta aatttttttg cttcttctgg aacatctttt tctaagcgtg caagtgatag
      541 ctttcctttt tcaagagaaa ccccatctaa cttattggaa ttggcagcta accactttaa
      601 cctttcatta aagctgctgg ttctctccgt tatataatct tcgaatgata aactaactga
      661 taatctcgta ttccccttcg attgattcca tgtatcttcc gaaaacaaat attcctcaaa
      721 atccctatat tgtctgctgc caacaatgga aacatctcct gcccgaacat gctcccgaag
      781 ttctgttaaa acagccattt catagtaatg acgattaatt gttgtaccat catcctcgta
      841 taaatgtctt ttccatcgtt ttgaaataaa atccacaggt gagtcatcag gcacttttcg
      901 ctttccagat tcgttcattc ctcggataat ctcaacagct tgtaaaagtg gctcatttgc
      961 ctttgtagaa tgaaattcca atactcttaa tagcgttggc gtatattttc ttagtgaata
     1021 aaaccgtttt tgcagtaagt ctaaataatc atagtcggca ggacgtgcaa gttcctgagc
     1081 ctcttctact gaagagacaa aggtattcca ttcaataacc gattctaaaa ccttaaaaac
     1141 gtctaatttt tcctctcttg ctttaattaa tgcttgtccg atgttcgtaa agtgtataac
     1201 tttctcattt agctttttac cgttttgttt ctggatttcc tcttgagcct tacgaccttt
     1261 tgataacaaa ctaagtattt gcctatcatg aatttcaaac gctttatccg ttagctcctg
     1321 agtaagttgt aataaataga tggttaatat cgaataacgt ttattttctt gaaagtcacg
     1381 gaatgcatac ggctcgtatc ttgagcctaa gcgagacagc tgcaacaggc ggttacggtg
     1441 caaatgacta atttgcactg tttctaaatc cattcctcgt atgtattcga gtcgttctat
     1501 tatttttaga aaagtttcgg gtgaaggatg acccggtggc tcttttaacc aacccaatat
     1561 cgttttattg gattcggatg gatgctgcga ggtaataatc ccttcaagct tttctttttg
     1621 ctcatttgtt agagatttac taaccgtatt aaatagcttc ttttcagcca ttgcccttgc
     1681 ttcccacacc attctttcaa gtgtagtgat agcaggcagt ataattttgt tttttcttag
     1741 aaaatctatg cattcatgca gtagatgaat ggcatcacca ttttccaaag ctaattgatg
     1801 aaggtactta aatgtcattc gatattcact cagggtaaaa gttacaaagt cgtattcact
     1861 tcgaatttct ttcaaatgat cccaaagtgt attttccctt tgaggataat gatcaagcga
     1921 ggatggacta acaccaatct gtttcgatat atattgtatg accgaatctg ggatgctttt
     1981 gatatgagtg tatggccaac cgggataccg aagaacagct aattgaacag caaatcctaa
     2041 acggttttct tccctccttc gcttattaac tatttctaaa tcccgtttgg aaaaagtgaa
     2101 gtaggtcccc agtatccatt catcttcagg gatttgcata aaagcctgtc tctgttccgg
     2161 tgtaagcaat tctctacctc tcgcaatttt cattcagtat cattccattt ctgtattttc
     2221 aatttattag ttcaattata tatcaataga gtgtactcta ttgatacaaa tgtagtagac
     2281 tgataaaatc atagttaaga gcgtctcata agacttgtct caaaaatgag gtgatatttt
     2341 gcggaaaatc ggttatattc gtgtcagttc gactaaccag aatccttcaa gacaatttca
     2401 gcagttgaac gagatcggaa tggatattat atatgaagag aaagtttcag gagcaacaaa
     2461 ggatcgcgag caacttcaaa aagtgttaga cgatttacag gaagatgaca tcatttatgt
     2521 tacagactta actcgaatca ctcgtagtac acaagatcta tttgaattaa tcgataacat
     2581 acgagataaa aaggcaagtt taaaatcact aaaagataca tggcttgatt tatcagaaga
     2641 taatccatac agccaattct taattactgt aatggctggt gttaaccaat tagagcgaga
     2701 tcttattcgg atgagacaac gtgaagggat tgaattggct aagaaagaag gaaagtttaa
     2761 aggtcgatta aagaagtatc ataaaaatca cgcaggaatg aattatgcgg taaagctata
     2821 taaagaagga aatatgactg taaatcaaat ttgtgaaatt actaatgtat ctagggcttc
     2881 attatacagg aaattatcag aagtgaataa ttagccattc tgtattccgc taatgggcaa
     2941 tatttttaaa gaagaaaagg aaactataaa atattaacag cctcctagcg atgccgaaaa
     3001 gccctttgat aaaaaaagaa tcatcatctt aagaaattct tagtcattta ttatgtaaat
     3061 gcttataaat tcggccctat aatctgataa attattaagg gcaaacttat gtgaaagggt
     3121 gataactatg agcgataaaa tacttattgt ggatgatgaa catgaaattg ccgatttggt
     3181 tgaattatac ttaaaaaacg agaattatac ggttttcaaa tactataccg ccaaagaagc
     3241 attggaatgt atagacaagt ctgagattga ccttgccata ttggacatca tgcttcccgg
     3301 cacaagcggc cttactatct gtcaaaaaat aagggacaag cacacctatc cgattatcat
     3361 gctgaccggg aaagatacag aggtagataa aattacaggg ttaacaatcg gcgcggatga
     3421 ttatataacg aagccctttc gcccactgga gttaattgct cgggtaaagg cccagttgcg
     3481 ccgatacaaa aaattcagtg gagtaaagga gcagaacgaa aatgttatcg tccactccgg
     3541 ccttgtcatt aatgttaaca cccatgagtg ttatctgaac gagaagcagt tatcccttac
     3601 tcccaccgag ttttcaatac tgcgaatcct ctgtgaaaac aaggggaatg tggttagctc
     3661 cgagctgcta tttcatgaga tatggggcga cgaatatttc agcaagagca acaacaccat
     3721 caccgtgcat atccggcatt tgcgcgaaaa aatgaacgac accattgata atccgaaata
     3781 tataaaaacg gtatgggggg ttggttataa aattgaaaaa taaaaaaaac gactattcca
     3841 aactagaacg aaaactttac atgtatatcg ttgcaattgt tgtggtagca attgtattcg
     3901 tgttgtatat tcgttcaatg atccgaggga aacttgggga ttggatctta agtattttgg
     3961 aaaacaaata tgacttaaat cacctggacg cgatgaaatt atatcaatat tccatacgga
     4021 acaatataga tatctttatt tatgtggcga ttgtcattag tattcttatt ctatgtcgcg
     4081 tcatgctttc aaaattcgca aaatactttg acgagataaa taccggcatt gatgtactta
     4141 ttcagaacga agataaacaa attgagcttt ctgcggaaat ggatgttatg gaacaaaagc
     4201 tcaacacatt aaaacggact ctggaaaagc gagagcagga tgcaaagctg gccgaacaaa
     4261 gaaaaaatga cgttgttatg tacttggcgc acgatattaa aacgcccctt acatccatta
     4321 tcggttattt gagcctgctt gacgaggctc cagacatgcc ggtagatcaa aaggcaaagt
     4381 atgtgcatat cacgttggac aaagcgtatc gactcgaaca gctaatcgac gagttttttg
     4441 agattacacg gtataaccta caaacgataa cgctaacaaa aacgcacata gacctatact
     4501 atatgctggt gcagatgacc gatgaatttt atcctcagct ttccgcacat ggaaaacagg
     4561 cggttattca cgcccccgag gatctgaccg tgtccggcga ccctgataaa ctcgcgagag
     4621 tctttaacaa cattttgaaa aacgccgctg catacagtga ggataacagc atcattgaca
     4681 ttaccgcggg cctctccggg gatgtggtgt caatcgaatt caagaacact ggaagcatcc
     4741 caaaagataa gctagctgcc atatttgaaa agttctatag gctggacaat gctcgttctt
     4801 ccgatacggg tggcgcggga cttggattgg cgattgcaaa agaaattatt gttcagcatg
     4861 gagggcagat ttacgcggaa agcaatgata actatacgac gtttagggta gagcttccag
     4921 cgatgccaga cttggttgat aaaaggaggt cctaagagat gtatataatt ttggctcttt
     4981 gtcaaatgat gttggtaaag agaaatct
//
LOCUS       DACEKH010000254         4864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.guided.136, whole genome shotgun sequence.
ACCESSION   DACEKH010000254 DACEKH010000000
VERSION     DACEKH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.guided.136"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            363..1274
                     /locus_tag="IW662_002877"
     CDS             363..1274
                     /locus_tag="IW662_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ0867641.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1427..1912
                     /gene="vanZ"
                     /locus_tag="IW662_002878"
     CDS             1427..1912
                     /gene="vanZ"
                     /locus_tag="IW662_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ0867642.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2199..2528)
                     /locus_tag="IW662_002879"
     CDS             complement(2199..2528)
                     /locus_tag="IW662_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0867643.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2833..3162
                     /locus_tag="IW662_002880"
     CDS             2833..3162
                     /locus_tag="IW662_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0867644.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            3152..3439
                     /locus_tag="IW662_002881"
     CDS             3152..3439
                     /locus_tag="IW662_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0867645.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3718..4281)
                     /locus_tag="IW662_002882"
     CDS             complement(3718..4281)
                     /locus_tag="IW662_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0867646.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1705 a          734 c          837 g         1588 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaaccattg aagatgtaag gcagagcgat
       61 attgcggtca ttatctgcgt gcgctgcggc aagatagcct gataataaga ctgatcgcat
      121 agaggggtgg tatttcacac cgcccattgt caacaggcag ttcagcctcg ttaaattcag
      181 catgggtatc acttatgaaa attcatctac attggtgata atagtaaatc cagtagggcg
      241 aaataattga ctgtaattta cggggcaaaa cggcacaatc tcaaacgaga ttgtgccgtt
      301 taaggggaag attctagaaa tatttcatac ttccaactat atagttaagg aggagactga
      361 aaatgaagaa gttgtttttt ttattgttat tgttattctt aatatactta ggttatgact
      421 acgttaatga agcactgttt tctcaggaaa aagtcgaatt tcaaaattat gatcaaaatc
      481 ccaaagaaca tttagaaaat agtgggactt ctgaaaatac ccaagagaaa acaattacag
      541 aagaacaggt ttatcaagga aatctgctat taatcaatag taaatatcct gttcgccaag
      601 aaagtgtgaa gtcagatatc gtgaatttat ctaaacatga cgaattaata aatggatacg
      661 ggttgcttga tagtaatatt tatatgtcaa aagaaatagc acaaaaattt tcagagatgg
      721 tcaatgatgc tgtaaagggt ggcgttagtc attttattat taatagtggc tatcgagact
      781 ttgatgagca aagtgtgctt taccaagaaa tgggggctga gtatgcctta ccagcaggtt
      841 atagtgagca taattcaggt ttatcactag atgtaggatc aagcttgacg aaaatggaac
      901 gagcccctga aggaaagtgg atagaagaaa atgcttggaa atacgggttc attttacgtt
      961 atccagagga caaaacagag ttaacaggaa ttcaatatga accatggcat attcgctatg
     1021 ttggtttacc acatagtgcg attatgaaag aaaagaattt cgttctcgag gaatatatgg
     1081 attacctaaa agaagaaaaa accatttctg ttagtgtaaa tggggaaaaa tatgagatct
     1141 tttattatcc tgttactaaa aataccacca ttcatgtgcc gactaatctt cgttatgaga
     1201 tatcaggaaa caatatagac ggtgtaattg tgacagtgtt tcccggatca acacatacta
     1261 attcaaggag gtaaggatgg cggaatgaaa ccaacgaaat taatgaacag cattattgta
     1321 ctagcacttt tggggtaacg ttagcttttt aatttaaaac ccacgttaac taggacattg
     1381 ctatactaat gatacaactt aaacaaaaga attagaggaa attatattgg gaaaaatatt
     1441 atctagagga ttgctagctt tatatttagt gacactaatc tggttagtgt tattcaaatt
     1501 acaatacaat attttatcag tatttaatta tcatcaaaga agtcttaact tgactccatt
     1561 tactgctact gggaatttca gagagatgat agataatgtt ataatcttta ttccatttgg
     1621 cttgcttttg aatgtcaatt ttaaagaaat cggattttta cctaagtttg cttttgtact
     1681 ggttttaagt cttacttttg aaataattca atttatcttc gctattggag cgacagacat
     1741 aacagatgta attacaaata ctgttggagg ctttcttgga ctgaaattat atggtttaag
     1801 caataagcat atgaatcaaa aaaaattaga cagagttatt atttttgtag gtatactttt
     1861 gctcgtatta ttgctcgttt accgtaccca tttaagaata aattacgtgt aagatgtcta
     1921 aatcaagcaa tctgatcttt catacacata aagatattga atgaattgga ttagatggaa
     1981 aacgggatgt ggggaaactc gcccgtaggt gtgaagtgag gggaaaaccg gtgataaagt
     2041 aaaaagctta cctaacacta tagtaacaaa gaaagcccaa ttatcaattt tagtgctgag
     2101 gaattggtct ctttaataaa tttccttaac gttgtaaatc cgcattttcc tgacggtacc
     2161 cccaaactaa gatttcatag atagattttc tgataacttt aaaaaacacg aatagcggaa
     2221 aggatgtctt cattcgctaa agagtaataa actacttttc catcttgacg tgattttgcc
     2281 attccatgtt tttttaaaaa acgtaaatgg tgtgacgttg tagcgacact agcagtaagt
     2341 atttctgcta aatcacagac gcacatttcc ttataggttt ctaaagcata gaaaatttta
     2401 attctagaaa aatcgctgaa acatttccct aacacaagta agtttgaaaa atctttttga
     2461 tccaatttat tttttattcc atttactttt tctttatgta cgacagtcac ttggcaaact
     2521 tttttcattc atctcacctc acattcaaac gattatttga atgaagtata cctatttgat
     2581 cactcttttg tcaatctacc ttaattccaa tacactatta taagcaattt catgtattga
     2641 agtaaactta cttgcaagtg cgtttttgat tccaagttaa aaacatatta ttattctaca
     2701 taatttccta atcaaataaa actcctttga aaattgttct aaaggagttt tattattttg
     2761 tatttctact aaaaatagcg tacaatgtaa ttactaagta attacgaaat gtaaaataat
     2821 ggaggaaata atatgattac aaaaacaaga aaacaaggaa attctattat gttaacggtc
     2881 cctaaagaat tcgatgtgcc caatggtgtt gaagtagaag ctaagcttgt agagaatggt
     2941 atcctttatg agtttgtaga gcctaaaaaa gaattttttg attttagtga agatattttg
     3001 gctgatattc tttcagaagg ttatagcaaa caagagatct taaaagaatt tagaaataga
     3061 aaaaatgcgt taacttctgc atttaaatct attgctgaag atactgttgc aaattctaaa
     3121 ccgatgacaa aagaggagtt ggcagcagaa attggcttat gagattctac cttctaaaca
     3181 cgtaataaag tatctaaaaa aattaaaaga gaaaacatta aaagaacaat ttttaacgat
     3241 aatttatgat gagatagcag ttcgtcctca ttcgggagaa caaaaaacgg gtgatttatc
     3301 aggaatttgg gctattggat tcaagtatgc aggtacgaca tatcgcgttg catatgaaat
     3361 taaagataat gcagtgatac ctatcttact atgtggaacg catgaaaatt tttatgaaca
     3421 attaaaaaag attaggtaag aacccaatgt ttcaacttga gaggatagac gacactttaa
     3481 aagataattt ttaacagctg agagttgtaa taccctataa ttttaaacca ctaacctata
     3541 tatttatgaa aaatagaaaa aattaaaacg cattttaagc ctctttcaaa ttaaaagctt
     3601 catccttatg cacaaagagg gcttaaaatg cgttatttta tattgttttt tagtaataat
     3661 aaaaaaatca ctctaattct cccataaata ttatagattc ctaaagatta aaatagatta
     3721 ttccctattg tcttttaagt agcggtagag tgttgccttg ctaataccag tcatttccgt
     3781 aatttctttt acgctataaa cctgactttt atacaatttt actgctcgat caacatcttt
     3841 ctggttcact cgtggacggc cacctttacg acctctcgca cgtgcacttt tcaaaccttc
     3901 tttggtacgt tcaacaatta aatcacgttc gaactgacta aatgcttgga agacagtcat
     3961 cattaagcgt ccgtgaggcg tgctggtatc gaagttttct ttcaagctca ccagcttaac
     4021 atggtgttct tcaaaatagg taacaaggtc aatcaaatcc tttgtactac gacccaatcg
     4081 agaaaaactt tctacaatta ccgtatctcc cggacgaatt ttatctttta aacgatttag
     4141 ttggggacga accgctttgg ttcccgtcat tttctcagtt aggatttcat tacaattttg
     4201 ttcattgagt aagtctaatt gacgatttag ttcttgtgaa cgtgtactca ctcgggcata
     4261 accataaatg tatccactca tgcttttctt cctttcgtac tttaattctg gcttgattgt
     4321 atcataaaag ggagttatag atacatagtt aatgcaacaa gttatgatac aaaatatgac
     4381 ctcttttatt taataaaaga ggtcatttca ttttgtctca taatcgattg tttttgatac
     4441 gagtgtgtat acgaaataaa caaaaaaatc ttaagtaaat ttctagatac ctaatttaat
     4501 cctaactagt gtatcctaaa gttaataacg agtttatcac aatgaaaatt ataaaatgct
     4561 cccatattag atataacatg caatatttga attagaacta caaaatttat tcgttttata
     4621 aagtaaataa tgttgagata ctaaggaata gaatattata aggaattgag ctgaatgtat
     4681 tggataaaaa aagaataact ttattagaga tggactataa aaatgctcgt aattttcttc
     4741 tgaagtctga aagctatttg aatgctcagc taccagaata tattagtttt gacgaaactt
     4801 taaaatctgc taaaaaagtt ttaacttcgc cgtttgtata attaagcgag acaaataaaa
     4861 aagc
//
LOCUS       DACEKH010000255         3360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.guided.164, whole genome shotgun sequence.
ACCESSION   DACEKH010000255 DACEKH010000000
VERSION     DACEKH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.guided.164"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            42..989
                     /locus_tag="IW662_002883"
     CDS             42..989
                     /locus_tag="IW662_002883"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0867647.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            1009..1536
                     /locus_tag="IW662_002884"
     CDS             1009..1536
                     /locus_tag="IW662_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0867648.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(1599..2252)
                     /locus_tag="IW662_002885"
     CDS             complement(1599..2252)
                     /locus_tag="IW662_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0867649.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            2384..3226
                     /locus_tag="IW662_002886"
     CDS             2384..3226
                     /locus_tag="IW662_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0867650.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
BASE COUNT         1186 a          600 c          687 g          887 t
ORIGIN      
        1 aaaactatta gacaaatgat attttacgag gaggaaaaag gatggaacaa gcgtatcttg
       61 acttaggaag aaaattgttg gaagaagggc atcgtaaaga agacagaaca ggcacaggca
      121 cattaagctt attcggatac caaatgcggt ttgatctgca aaaagggttt cctttgttaa
      181 caacgaaacg agtacctttt ggattgatca aaagcgaatt gctttggttt ttaaaaggag
      241 atacgaatat ccgctatcta ttacaaaata aaaaccatat ttgggatgaa tgggcatttg
      301 aacgctacat caaaagtcca gactataatg gaccagatat gactgatttt ggccatagat
      361 gtttgacaga tcctgaattc aatgaagaat ataaaaagca gagcaagctt ttttgcgaga
      421 agatcctaac cgatgattcc tttgctgaaa catacggcga tcttggacac atctacggct
      481 atcaatggcg tcactgggaa acaaaagacg gtggctttat cgatcaaatc aaacaagtga
      541 tcgaagcaat caaaaagact cctgattcta gaagattgat cgtttctgca tggaatccgg
      601 aagatgttcc tagcatggct ttgcctcctt gtcatacgat gtttcaattt tatgttcaag
      661 agggacggct aagctgccaa ttgtatcaac gcagtgggga tgtgtttcta ggcgttccgt
      721 tcaacattgc aagttatgcg ttgctgactc acctaattgc acatgaaaca ggtttagcgg
      781 ttggcgaatt tgttcacaca ttaggagatg ctcatcttta tatcaatcat atcgaacaaa
      841 tgaaagaaca attgagccga gaaatacgcg catttccgac tttagttttg aatcaggaaa
      901 agcagtcagt atttgatttt gagatggaag atatccaatt agaaggatat gacccacatc
      961 cggcgatcaa agctcctatc gctgtttaga atcagaaagg atgagaaaat gttagctgca
     1021 atttgggcac aggatgaaaa cggattgatt ggaaaagaag accgtcttcc ttggcgtttg
     1081 ccaaatgatc tgaaattctt caaacaaatg acagaagcaa atacactagt gatgggacga
     1141 aaaacattcg aagggatggg tagccgtccg ttgccgaatc gtcagacgat tgttttgacg
     1201 agagattctt cctataaagc agaaggcgtg catgtcatgc atagtgtgga agacgtgctt
     1261 gctctggaaa aagaaacaga cggtatcttt ttcatcgctg gagggtcagc tgtttatcaa
     1321 gaatttctgc cattttgtac gatattatac cgtacagtca tccatcatgt attcgatgga
     1381 gatgcttatt ttccacctgt tgactggtct gattggtcac tgatcaatac tagtcaagga
     1441 gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa cgtatcagcg taatgagaat
     1501 cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa gctatggata gactagctgt
     1561 tatttagagc tgtcccatcc atagctttat tttttttatt aagtaaagaa taaaacagag
     1621 aagtacataa aacctgcacc taacataaca aacaaatgcc aaacaacatg catatagcgg
     1681 atagaattca agctataaaa gaatgcgcct atcgtgtaag aaagcccgcc tgctaccatc
     1741 aaaataacac cagtcattcc aagagagtga taaagaggaa cagcagccac gacacagagc
     1801 caccccataa aaatatagat gatcgtagat acttttgata ctctctcccg tttatgcaac
     1861 gtcaacgctt tatatataat gccaagaatc gcaaacaacc agattaatac aaataaagtc
     1921 caacctagcc atcctcggat actcagcaaa caaaaaggag tataacttcc tgcaatcaaa
     1981 agaaaaatcg aactatgatc gaatacttgg aacacctttt ttgcttttgt aaaaatcaat
     2041 gaatgaaaca aagtagacgt taaaaataaa agaatcatca tcgatccata gatagcataa
     2101 gagacaatat ggagggagga atgcaagtgg gcacctttta ctaaaagaac cactagtccc
     2161 gcgatactca atccagtccc aattccatgg gtgactgcat taaacacttc atttaatatt
     2221 tcatacgttc gattaaattt cgttttttcc atgaaaacgg ctcctttact aggtgtaatt
     2281 aatgaaaaat cattatttgt ctgttatact aaaaatagaa attgtcttac atgtagttta
     2341 acatgaataa ggcatcttgt taaagaaaga gagagagaaa ataatgacaa atgtaaaaat
     2401 cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc gatgctttag gcattcgtgt
     2461 catgccgcta tccatcatga tcgacggaac agtatatccc gatgatgacc aactagacgg
     2521 cgaaagattt atggaaatga tggcgaaagc aaaaaatctg cccaaaacaa gccaaccacc
     2581 aattggagaa ttcatctcga tgtatgatga gctaggagaa gacggcagtg aagtgatttc
     2641 gatccatatg acaaaaggct tgagtggaac agtagaagca gcaagacaag ccagtaatct
     2701 aacaaaaacg aaagtaacgg ttatcgacag tgataccact gaccaaggat tatcttttca
     2761 ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca gtagagaaaa tccttgaaga
     2821 agtcaaaaaa gtacaagaca atacaaaatt atacatcgga atatccacac tagataactt
     2881 ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg tctagtgttt tgaacatacg
     2941 cgtggtgatg aatttcgccc attctgaatt aatccctgta actaaggggc gaggaaagaa
     3001 aacgttcgat aaatggttta ataatttgaa aaatgaactg aaagaattgc caaatgtgcg
     3061 acaaatcggt atttcacatg cggatgcaaa agaattggcg caagaattca aagagggttt
     3121 gcaagaatta ttcccggaca tgcatatccc tgtactgcat acgaatccgg ttatcgctac
     3181 gcatactggt aaagacgcgt ttgcaatcat gtattatgta gattagtcga aaagaggata
     3241 agccctcagc ctgaaaggct tgcagcttat tctctttttc tttataaaat taggaaaata
     3301 attgttttct tttttcataa agactacctt tttgataaaa tttttgataa cattagaacc
//
LOCUS       DACEKH010000256         3000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.guided.172, whole genome shotgun sequence.
ACCESSION   DACEKH010000256 DACEKH010000000
VERSION     DACEKH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.guided.172"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            246..1214
                     /locus_tag="IW662_002887"
     CDS             246..1214
                     /locus_tag="IW662_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ0867651.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1207..2238
                     /gene="vanA"
                     /locus_tag="IW662_002888"
     CDS             1207..2238
                     /gene="vanA"
                     /locus_tag="IW662_002888"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ0867652.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2244..2852
                     /locus_tag="IW662_002889"
     CDS             2244..2852
                     /locus_tag="IW662_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0867653.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          894 a          574 c          723 g          809 t
ORIGIN      
        1 gatgctggtg tcatccacca acatcaaaaa gtatagagcc ataatttttt aggaaaatct
       61 caaggttatc tttacttttt cttaggaaat taacaattta atattaagaa acggctcgtt
      121 cttacacggt agacttaata ccgtaagaac gagccgtttt cgttcttcag agaaagattt
      181 gacaagatta ccattggcat ccccgtttta tttggtgcct ttcacagaaa gggttggtct
      241 taattatgaa taacatcggc attactgttt atggatgtga gcaggatgag gcagatgcat
      301 tccatgctct ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc aacgtgtcgg
      361 aatccaacgc caaatccgcg cctttcaatc aatgtatcag tgtgggacat aaatcagaga
      421 tttccgcctc tattcttctt gcgctgaaga gagccggtgt gaaatatatt tctacccgaa
      481 gcatcggctg caatcatata gatacaactg ctgctaagag aatgggcatc actgtcgaca
      541 atgtggcgta ctcgccggat agcgttgccg attatactat gatgctaatt cttatggcag
      601 tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg ttggacagcg
      661 accgtggcaa ggtactcagc gacatgacag ttggtgtggt gggaacgggc cagataggca
      721 aagcggttat tgagcggctg cgaggatttg gatgtaaagt gttggcttat agtcgcagcc
      781 gaagtataga ggtaaactat gtaccgtttg atgagttgct gcaaaatagc gatatcgtta
      841 cgcttcatgt gccgctcaat acggatacgc actatattat cagccacgaa caaatacaga
      901 gaatgaagca aggagcattt cttatcaata ctgggcgcgg tccacttgta gatacctatg
      961 agttggttaa agcattagaa aacgggaaac tgggcggtgc cgcattggat gtattggaag
     1021 gagaggaaga gtttttctac tctgattgca cccaaaaacc aattgataat caatttttac
     1081 ttaaacttca aagaatgcct aacgtgataa tcacaccgca tacggcctat tataccgagc
     1141 aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg tttggatttt gaaaggagac
     1201 aggagcatga atagaataaa agttgcaata ctgtttgggg gttgctcaga ggagcatgac
     1261 gtatcggtaa aatctgcaat agagatagcc gctaacatta ataaagaaaa atacgagccg
     1321 ttatacattg gaattacgaa atctggtgta tggaaaatgt gcgaaaaacc ttgcgcggaa
     1381 tgggaaaacg acaattgcta ttcagctgta ctctcgccgg ataaaaaaat gcacggatta
     1441 cttgttaaaa agaaccatga atatgaaatc aaccatgttg atgtagcatt ttcagctttg
     1501 catggcaagt caggtgaaga tggatccata caaggtctgt ttgaattgtc cggtatccct
     1561 tttgtaggct gcgatattca aagctcagca atttgtatgg acaaatcgtt gacatacatc
     1621 gttgcgaaaa atgctgggat agctactccc gccttttggg ttattaataa agatgatagg
     1681 ccggtggcag ctacgtttac ctatcctgtt tttgttaagc cggcgcgttc aggctcatcc
     1741 ttcggtgtga aaaaagtcaa tagcgcggac gaattggact acgcaattga atcggcaaga
     1801 caatatgaca gcaaaatctt aattgagcag gctgtttcgg gctgtgaggt cggttgtgcg
     1861 gtattgggaa acagtgccgc gttagctgtt ggcgaggtgg accaaatcag gctgcagtac
     1921 ggaatctttc gtattcatca ggaagtcgag ccggaaaaag gctctgaaaa cgcagttata
     1981 accgttcccg cagacctttc agcagaggag cgaggacgga tacaggaaac ggcaaaaaaa
     2041 atatataaag cgctcggctg tagaggtcta gcccgtgtgg atatgttttt acaagataac
     2101 ggccgcattg tactgaacga agtcaatact ctgcccggtt tcacgtcata cagtcgttat
     2161 ccccgtatga tggccgctgc aggtattgca cttcccgaac tgattgaccg cttgatcgta
     2221 ttagcgttaa aggggtgata agcatggaaa taggatttac ttttttagat gaaatagtac
     2281 acggtgttcg ttgggacgct aaatatgcca cttgggataa tttcaccgga aaaccggttg
     2341 acggttatga agtaaatcgc attgtaggga catacgagtt ggctgaatcg cttttgaagg
     2401 caaaagaact ggctgctacc caagggtacg gattgcttct atgggacggt taccgtccta
     2461 atcgtgctgt aaactgtttt atgcaatggg ctgcacagcc ggaaaataac ctgacaaagg
     2521 aaagttatta tcccaatatt gaccgaactg agatgatttc aaaaggatac gtggcttcaa
     2581 aatcaagcca tagccgcggc agtgccattg atcttacgct ttatcgatta gacacgggtg
     2641 agcttgtacc aatggggagc cgatttgatt ttatggatga acgctctcat catgcggcaa
     2701 atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt gcgctccatc atggaaaaca
     2761 gtgggtttga agcatatagc ctcgaatggt ggcactatgt attaagagac gaaccatacc
     2821 ccaatagcta ttttgatttc cccgttaaat aaacttttaa ccgttgcacg gacaaactat
     2881 ataagctaac tctttcggca ggaaacccga cgtatgtaac tggttcttag ggaatttata
     2941 tatagtagat agtattgaag aggttctgtt gcaaagtttt aaataaagaa taaaatccct
//
LOCUS       DACEKH010000257         2309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.guided.188, whole genome shotgun sequence.
ACCESSION   DACEKH010000257 DACEKH010000000
VERSION     DACEKH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.guided.188"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            49..591
                     /gene="sat4"
                     /locus_tag="IW662_002890"
     CDS             49..591
                     /gene="sat4"
                     /locus_tag="IW662_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ0867654.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            684..1478
                     /locus_tag="IW662_002891"
                     /note="aph(3')-IIIa"
     CDS             684..1478
                     /locus_tag="IW662_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0867655.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(1586..1774)
                     /locus_tag="IW662_002892"
                     /pseudo
     CDS             complement(1586..1774)
                     /locus_tag="IW662_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1754..2296
                     /locus_tag="IW662_002893"
     CDS             1754..2296
                     /locus_tag="IW662_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0867656.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT          786 a          379 c          539 g          605 t
ORIGIN      
        1 caaaaccgga aggttaaaac cctaactcta atatacaagg cgggaacagt gattacagaa
       61 atgaaagcag ggcacctgaa agatatcgat aaacccagcg aaccatttga ggtgataggt
      121 aagattatac cgaggtatga aaacgagaat tggaccttta cagaattact ctatgaagcg
      181 ccatatttaa aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt
      241 gaatatattg acaatactga taagataata tatctttact accaagacga taaatgcgtc
      301 ggaaaagtta aactgcgaaa aaattggaac cggtacgctt atatagaaga tatcgccgta
      361 tgtaaggatt tcagggggca aggcataggc agcgcgctta tcaatatatc tatagaatgg
      421 gcaaagcata aaaacttgca tggactaatg cttgaaaccc aggacaataa ccttatagct
      481 tgtaaattct atcataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc
      541 aactttgaaa acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg
      601 aacagtgaat tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt
      661 agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac
      721 tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata
      781 agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga
      841 ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg
      901 ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg
      961 ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg
     1021 agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata
     1081 cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg
     1141 atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg
     1201 attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag
     1261 acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg
     1321 gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg
     1381 gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga
     1441 aaataaaata ttatatttta ctggatgaat tgttttagta cctagattta gatgtctaaa
     1501 aagctttaac tacaagcttt ttagacatct aatcttttct gaagtacatc cgcaactgtc
     1561 catactctga tgttttatat cttttctaaa agttcgctag atagagttct atattagaga
     1621 tgtaaagagt tttggtgaag aggtttgtga gtatggcttt tataaccagg gtgcaatgag
     1681 aagcactaac agcagctagc ttaagaacgc ataggatata ctttttatgg aagtcacaga
     1741 ggaggaataa aatttgaaca ggattaacag agtaacctct attcttattc agctgcaatc
     1801 aaaaaaaata attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt
     1861 ttacagagat atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa
     1921 aggatatttt cttgtagagg gcttcctact tccgccggta atgtttacag cggcggaagt
     1981 gggtgcattg attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa
     2041 ggattttgat tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta
     2101 tgcacaggag ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt
     2161 ggcggataac gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat
     2221 tcaatatcct gcatcaggag ggcaagaacc ggaaagcaga atgatagagc cggttctgtt
     2281 gcaaagtttt aaataaagaa taaaatccc
//
LOCUS       DACEKH010000258         1739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.guided.207, whole genome shotgun sequence.
ACCESSION   DACEKH010000258 DACEKH010000000
VERSION     DACEKH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.guided.207"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..977
                     /gene="vanA"
                     /locus_tag="IW662_002894"
     CDS             <1..977
                     /gene="vanA"
                     /locus_tag="IW662_002894"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase"
                     /protein_id="HAQ0867657.1"
                     /translation="SVKSAIEIAANIKKKKYEPLYIGITKSGVWKMCEKPCAEWENDN
                     CYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKSGEDGSIQGLFELSGIPFVG
                     CDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPVAATFTYPVFVKPARSGSSF
                     GVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCAVLGNSAALAVGEVDQIRLQ
                     YGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETAKKIYKALGCRGLARVDMFL
                     QDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELIDRLIVLALKG"
     gene            983..1591
                     /locus_tag="IW662_002895"
     CDS             983..1591
                     /locus_tag="IW662_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0867658.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          522 a          333 c          422 g          462 t
ORIGIN      
        1 tatcggtaaa atctgcaata gagatagccg ctaacattaa aaaaaaaaaa tacgagccgt
       61 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
      121 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
      181 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
      241 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
      301 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
      361 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
      421 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
      481 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
      541 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
      601 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
      661 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
      721 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
      781 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
      841 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
      901 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
      961 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     1021 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     1081 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     1141 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     1201 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     1261 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     1321 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     1381 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     1441 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     1501 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     1561 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     1621 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     1681 atagtagata gtattgaaga ggttctgttg caaagtttta aataaagaat aaaatccct
//
LOCUS       DACEKH010000259         1715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.guided.208, whole genome shotgun sequence.
ACCESSION   DACEKH010000259 DACEKH010000000
VERSION     DACEKH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.guided.208"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..953
                     /gene="vanA"
                     /locus_tag="IW662_002896"
     CDS             <1..953
                     /gene="vanA"
                     /locus_tag="IW662_002896"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase"
                     /protein_id="HAQ0867659.1"
                     /translation="AANIKKKKYEPLYIGITKSGVWKMCEKPCAEWENDNCYSAVLSP
                     DKKMHGLLVKKNHEYEINHVDVAFSALHGKSGEDGSIQGLFELSGIPFVGCDIQSSAI
                     CMDKSLTYIVAKNAGIATPAFWVINKDDRPVAATFTYPVFVKPARSGSSFGVKKVNSA
                     DELDYAIESARQYDSKILIEQAVSGCEVGCAVLGNSAALAVGEVDQIRLQYGIFRIHQ
                     EVEPEKGSENAVITVPADLSAEERGRIQETAKKIYKALGCRGLARVDMFLQDNGRIVL
                     NEVNTLPGFTSYSRYPRMMAAAGIALPELIDRLIVLALKG"
     gene            959..1567
                     /locus_tag="IW662_002897"
     CDS             959..1567
                     /locus_tag="IW662_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0867660.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          513 a          330 c          417 g          455 t
ORIGIN      
        1 tagccgctaa cataaaaaaa aaaaaatacg agccgttata cattggaatt acgaaatctg
       61 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
      121 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
      181 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
      241 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
      301 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
      361 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
      421 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
      481 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
      541 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
      601 ctgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
      661 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
      721 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
      781 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
      841 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
      901 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
      961 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     1021 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     1081 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     1141 gtacggattg cttctatggg acggttaccg tcctaatcgt gctgtaaact gttttatgca
     1201 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     1261 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcagtgc
     1321 cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg ggagccgatt
     1381 tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca atgaagcgca
     1441 aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat atagcctcga
     1501 atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg atttccccgt
     1561 taaataaact tttaaccgtt gcacggacaa actatataag ctaactcttt cggcaggaaa
     1621 cccgacgtat gtaactggtt cttagggaat ttatatatag tagatagtat tgaagaggtt
     1681 ctgttgcaaa gttttaaata aagaataaaa tccct
//
LOCUS       DACEKH010000260          936 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.guided.244, whole genome shotgun sequence.
ACCESSION   DACEKH010000260 DACEKH010000000
VERSION     DACEKH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.guided.244"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..634
                     /locus_tag="IW662_002898"
     CDS             <1..634
                     /locus_tag="IW662_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HAQ0867661.1"
                     /translation="SILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLD
                     YTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLC
                     HNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMY
                     GNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(764..>936)
                     /locus_tag="IW662_002899"
     CDS             complement(764..>936)
                     /locus_tag="IW662_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0867662.1"
                     /translation="KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEW
                     IYSSRKYINFDK"
BASE COUNT          373 a           85 c          160 g          318 t
ORIGIN      
        1 atctatacta ggttataaag aaattaaagg aactttttta acaccagaaa tttattctac
       61 tatgtcagaa gaagaacaaa atttgttaaa acgagatatt gccagttttt taagacaaat
      121 gcacggttta gattatacag atattagtga atgtactatt gataataaac aaaatgtatt
      181 agaagagtat atattgttgc gtgaaactat ttataatgat ttaactgata tagaaaaaga
      241 ttatatagaa agttttatgg aaagactaaa tgcaacaaca gtttttgagg gtaaaaagtg
      301 tttatgccat aatgatttta gttgtaatca tctattgtta gatggcaata atagattaac
      361 tggaataatt gattttggag attctggaat tatagatgaa tattgtgatt ttatatactt
      421 acttgaagat agtgaagaag aaataggaac aaattttgga gaagatatat taagaatgta
      481 tggaaatata gatattgaga aagcaaaaga atatcaagat atagttgaag aatattatcc
      541 tattgaaact attgtttatg gaattaaaaa tattaaacag gaatttatcg aaaatggtag
      601 aaaagaaatt tataaaagga cttataaaga ttgattatat aatatatgaa aagctattat
      661 aaaagacatt agtattaaat agtttaaaaa aatgaaaaat aataaaggaa gtgagtcaag
      721 tccagactcc tgtgtaaaat gctatacaat gtttttacca tttctactta tcaaaattga
      781 tgtattttct tgaagaataa atccattcat catgtaggtc cataagaacg gctccaatta
      841 agcgattggc tgatgtttga ttggggaaga tgcgaataat cttttctctt ctgcgtactt
      901 cttgattcag tcgttcaatt agattggtac tcttta
//
LOCUS       DACEKH010000261          804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.guided.247, whole genome shotgun sequence.
ACCESSION   DACEKH010000261 DACEKH010000000
VERSION     DACEKH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.guided.247"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            191..739
                     /gene="aac(6')"
                     /locus_tag="IW662_002900"
     CDS             191..739
                     /gene="aac(6')"
                     /locus_tag="IW662_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0867663.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          285 a          121 c          174 g          224 t
ORIGIN      
        1 tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt
       61 tttttatttt ataagtaaga taaataatga aagaatagag atactgattg gtagctaaag
      121 atttttgagc gagaacattt cgttttttca aaaggactgt tagaataagc aagaacaagg
      181 gggaagacgt atgataatca gtgaatttga ccgtaataat ccagtattga aagatcagct
      241 ttctgattta ctgagactga cttggccgga agaatatgga gacagctcgg cagaagaagt
      301 agaagaaatg atgaatccag aacgaatcgc ggtagcagcg gtagaccaag atgagttagt
      361 aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt tgggaattgc atccattagt
      421 tgtagaaagc tcccgacgaa agaaccaaat aggtactcga ttagtcaatt acttagaaaa
      481 agaagtagct tccagaggag gaatcacgat ttatttaggt acggatgatt tagaccatgg
      541 aacaacgtta agtcaaacgg acctgtatga gcatacattt gataaagtgg cttccatcca
      601 gaaccttcgt gaacatccgt atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt
      661 cttaccaaat gcaaatggct gggacaaacc ggatatttgg atggcaaaaa cgattattcc
      721 tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata
      781 agaaggaatt tacgaaaatt actt
//
LOCUS       DACEKH010000262          563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E293
            SAMN07141399-rid9869293.guided.261, whole genome shotgun sequence.
ACCESSION   DACEKH010000262 DACEKH010000000
VERSION     DACEKH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141399
            Sequence Read Archive: SRR5811538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740868.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740868.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 21:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,871
            Genes (coding)                    :: 2,805
            CDSs (with protein)               :: 2,805
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 30 of 66
            Pseudo Genes (incomplete)         :: 30 of 66
            Pseudo Genes (internal stop)      :: 15 of 66
            Pseudo Genes (multiple problems)  :: 8 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141399-rid9869293.guided.261"
                     /isolate="E293"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Apr-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>563
                     /locus_tag="IW662_002901"
     CDS             <1..>563
                     /locus_tag="IW662_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0867664.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYL"
BASE COUNT          224 a           53 c          103 g          183 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tag
//
